LOCUS       DACEDO010000001       103331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEDO010000001 DACEDO010000000
VERSION     DACEDO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 103331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 103331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.001"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1032)
                     /locus_tag="IWO52_000001"
     CDS             complement(<1..1032)
                     /locus_tag="IWO52_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0354303.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVL"
     gene            complement(1061..1378)
                     /locus_tag="IWO52_000002"
     CDS             complement(1061..1378)
                     /locus_tag="IWO52_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0354304.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(1404..1724)
                     /locus_tag="IWO52_000003"
     CDS             complement(1404..1724)
                     /locus_tag="IWO52_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0354305.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(2189..3604)
                     /locus_tag="IWO52_000004"
     CDS             complement(2189..3604)
                     /locus_tag="IWO52_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0354306.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(3621..4937)
                     /locus_tag="IWO52_000005"
     CDS             complement(3621..4937)
                     /locus_tag="IWO52_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0354307.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(5087..5785)
                     /locus_tag="IWO52_000006"
     CDS             complement(5087..5785)
                     /locus_tag="IWO52_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0354308.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(6074..7063)
                     /locus_tag="IWO52_000007"
     CDS             complement(6074..7063)
                     /locus_tag="IWO52_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0354309.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(7143..7976)
                     /gene="msrA"
                     /locus_tag="IWO52_000008"
     CDS             complement(7143..7976)
                     /gene="msrA"
                     /locus_tag="IWO52_000008"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0354310.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(8151..9785)
                     /locus_tag="IWO52_000009"
     CDS             complement(8151..9785)
                     /locus_tag="IWO52_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354311.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(9772..11619)
                     /locus_tag="IWO52_000010"
     CDS             complement(9772..11619)
                     /locus_tag="IWO52_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354312.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            11606..13243
                     /locus_tag="IWO52_000011"
     CDS             11606..13243
                     /locus_tag="IWO52_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0354313.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(13388..14128)
                     /gene="truA"
                     /locus_tag="IWO52_000012"
     CDS             complement(13388..14128)
                     /gene="truA"
                     /locus_tag="IWO52_000012"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0354314.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(14319..14954)
                     /locus_tag="IWO52_000013"
     CDS             complement(14319..14954)
                     /locus_tag="IWO52_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0354315.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(15101..16342)
                     /gene="sstT"
                     /locus_tag="IWO52_000014"
     CDS             complement(15101..16342)
                     /gene="sstT"
                     /locus_tag="IWO52_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0354316.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            16540..17538
                     /locus_tag="IWO52_000015"
     CDS             16540..17538
                     /locus_tag="IWO52_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354317.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            17551..19569
                     /locus_tag="IWO52_000016"
     CDS             17551..19569
                     /locus_tag="IWO52_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ0354318.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(19629..20345)
                     /locus_tag="IWO52_000017"
     CDS             complement(19629..20345)
                     /locus_tag="IWO52_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0354319.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(20572..21114)
                     /locus_tag="IWO52_000018"
     CDS             complement(20572..21114)
                     /locus_tag="IWO52_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0354320.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(21119..21346)
                     /locus_tag="IWO52_000019"
     CDS             complement(21119..21346)
                     /locus_tag="IWO52_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ0354321.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(21425..23305)
                     /locus_tag="IWO52_000020"
     CDS             complement(21425..23305)
                     /locus_tag="IWO52_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0354322.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTISVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(23494..24108)
                     /locus_tag="IWO52_000021"
     CDS             complement(23494..24108)
                     /locus_tag="IWO52_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0354323.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            24250..24810
                     /locus_tag="IWO52_000022"
     CDS             24250..24810
                     /locus_tag="IWO52_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0354324.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(25115..25429)
                     /locus_tag="IWO52_000023"
                     /pseudo
     CDS             complement(25115..25429)
                     /locus_tag="IWO52_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(25683..27318)
                     /locus_tag="IWO52_000024"
                     /pseudo
     CDS             complement(25683..27318)
                     /locus_tag="IWO52_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27641..28120)
                     /gene="rlmH"
                     /locus_tag="IWO52_000025"
     CDS             complement(27641..28120)
                     /gene="rlmH"
                     /locus_tag="IWO52_000025"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0354325.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28548..29837
                     /locus_tag="IWO52_000026"
     CDS             28548..29837
                     /locus_tag="IWO52_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0354326.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(29876..30481)
                     /locus_tag="IWO52_000027"
     CDS             complement(29876..30481)
                     /locus_tag="IWO52_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0354327.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(30686..31150)
                     /locus_tag="IWO52_000028"
     CDS             complement(30686..31150)
                     /locus_tag="IWO52_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0354328.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(31172..31492)
                     /locus_tag="IWO52_000029"
     CDS             complement(31172..31492)
                     /locus_tag="IWO52_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0354329.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(31636..32715)
                     /gene="pepA"
                     /locus_tag="IWO52_000030"
     CDS             complement(31636..32715)
                     /gene="pepA"
                     /locus_tag="IWO52_000030"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0354330.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            32915..33265
                     /locus_tag="IWO52_000031"
     CDS             32915..33265
                     /locus_tag="IWO52_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0354331.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            33488..34435
                     /locus_tag="IWO52_000032"
     CDS             33488..34435
                     /locus_tag="IWO52_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0354332.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(34492..35910)
                     /gene="pepV"
                     /locus_tag="IWO52_000033"
     CDS             complement(34492..35910)
                     /gene="pepV"
                     /locus_tag="IWO52_000033"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0354333.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            36059..36904
                     /locus_tag="IWO52_000034"
     CDS             36059..36904
                     /locus_tag="IWO52_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0354334.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            36908..37348
                     /locus_tag="IWO52_000035"
     CDS             36908..37348
                     /locus_tag="IWO52_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0354335.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            37398..37592
                     /locus_tag="IWO52_000036"
     CDS             37398..37592
                     /locus_tag="IWO52_000036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354336.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            37728..38414
                     /locus_tag="IWO52_000037"
     CDS             37728..38414
                     /locus_tag="IWO52_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0354337.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(38822..39511)
                     /locus_tag="IWO52_000038"
     CDS             complement(38822..39511)
                     /locus_tag="IWO52_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354338.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(39486..41615)
                     /locus_tag="IWO52_000039"
     CDS             complement(39486..41615)
                     /locus_tag="IWO52_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0354339.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(41663..43000)
                     /locus_tag="IWO52_000040"
     CDS             complement(41663..43000)
                     /locus_tag="IWO52_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0354340.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(43252..43611)
                     /gene="rplT"
                     /locus_tag="IWO52_000041"
     CDS             complement(43252..43611)
                     /gene="rplT"
                     /locus_tag="IWO52_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0354341.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(43664..43864)
                     /gene="rpmI"
                     /locus_tag="IWO52_000042"
     CDS             complement(43664..43864)
                     /gene="rpmI"
                     /locus_tag="IWO52_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0354342.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(43936..44460)
                     /gene="infC"
                     /locus_tag="IWO52_000043"
     CDS             complement(43936..44460)
                     /gene="infC"
                     /locus_tag="IWO52_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0354343.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            44780..45412
                     /locus_tag="IWO52_000044"
     CDS             44780..45412
                     /locus_tag="IWO52_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0354344.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(45414..46319)
                     /locus_tag="IWO52_000045"
     CDS             complement(45414..46319)
                     /locus_tag="IWO52_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0354345.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(46404..47057)
                     /locus_tag="IWO52_000046"
     CDS             complement(46404..47057)
                     /locus_tag="IWO52_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0354346.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            47379..48323
                     /gene="mvk"
                     /locus_tag="IWO52_000047"
     CDS             47379..48323
                     /gene="mvk"
                     /locus_tag="IWO52_000047"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0354347.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            48316..49293
                     /gene="mvaD"
                     /locus_tag="IWO52_000048"
     CDS             48316..49293
                     /gene="mvaD"
                     /locus_tag="IWO52_000048"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0354348.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            49306..50391
                     /locus_tag="IWO52_000049"
     CDS             49306..50391
                     /locus_tag="IWO52_000049"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0354349.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            50388..51452
                     /locus_tag="IWO52_000050"
     CDS             50388..51452
                     /locus_tag="IWO52_000050"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0354350.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(51701..52504)
                     /locus_tag="IWO52_000051"
     CDS             complement(51701..52504)
                     /locus_tag="IWO52_000051"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0354351.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(52524..53366)
                     /gene="kduI"
                     /locus_tag="IWO52_000052"
     CDS             complement(52524..53366)
                     /gene="kduI"
                     /locus_tag="IWO52_000052"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ0354352.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(53382..54389)
                     /locus_tag="IWO52_000053"
     CDS             complement(53382..54389)
                     /locus_tag="IWO52_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ0354353.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(54396..55040)
                     /locus_tag="IWO52_000054"
     CDS             complement(54396..55040)
                     /locus_tag="IWO52_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0354354.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(55140..55898)
                     /locus_tag="IWO52_000055"
     CDS             complement(55140..55898)
                     /locus_tag="IWO52_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ0354355.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(56025..57176)
                     /gene="fucO"
                     /locus_tag="IWO52_000056"
     CDS             complement(56025..57176)
                     /gene="fucO"
                     /locus_tag="IWO52_000056"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ0354356.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(57258..57569)
                     /gene="rhaM"
                     /locus_tag="IWO52_000057"
     CDS             complement(57258..57569)
                     /gene="rhaM"
                     /locus_tag="IWO52_000057"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ0354357.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(57571..58416)
                     /gene="rhaD"
                     /locus_tag="IWO52_000058"
     CDS             complement(57571..58416)
                     /gene="rhaD"
                     /locus_tag="IWO52_000058"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ0354358.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(58430..59686)
                     /gene="rhaA"
                     /locus_tag="IWO52_000059"
     CDS             complement(58430..59686)
                     /gene="rhaA"
                     /locus_tag="IWO52_000059"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ0354359.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(59683..61143)
                     /gene="rhaB"
                     /locus_tag="IWO52_000060"
     CDS             complement(59683..61143)
                     /gene="rhaB"
                     /locus_tag="IWO52_000060"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ0354360.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            61296..62165
                     /locus_tag="IWO52_000061"
     CDS             61296..62165
                     /locus_tag="IWO52_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0354361.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(62291..63190)
                     /locus_tag="IWO52_000062"
     CDS             complement(62291..63190)
                     /locus_tag="IWO52_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0354362.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(63355..64179)
                     /locus_tag="IWO52_000063"
     CDS             complement(63355..64179)
                     /locus_tag="IWO52_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0354363.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(64190..65485)
                     /locus_tag="IWO52_000064"
     CDS             complement(64190..65485)
                     /locus_tag="IWO52_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ0354364.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(65501..65980)
                     /locus_tag="IWO52_000065"
     CDS             complement(65501..65980)
                     /locus_tag="IWO52_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0354365.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(65986..67014)
                     /locus_tag="IWO52_000066"
     CDS             complement(65986..67014)
                     /locus_tag="IWO52_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ0354366.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(67031..68137)
                     /locus_tag="IWO52_000067"
     CDS             complement(67031..68137)
                     /locus_tag="IWO52_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0354367.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(68366..70963)
                     /gene="adhE"
                     /locus_tag="IWO52_000068"
                     /gene_synonym="adhC"
     CDS             complement(68366..70963)
                     /gene="adhE"
                     /locus_tag="IWO52_000068"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0354368.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(71268..71648)
                     /gene="yajC"
                     /locus_tag="IWO52_000069"
     CDS             complement(71268..71648)
                     /gene="yajC"
                     /locus_tag="IWO52_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0354369.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(71745..72890)
                     /gene="tgt"
                     /locus_tag="IWO52_000070"
     CDS             complement(71745..72890)
                     /gene="tgt"
                     /locus_tag="IWO52_000070"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0354370.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            73108..73806
                     /locus_tag="IWO52_000071"
     CDS             73108..73806
                     /locus_tag="IWO52_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0354371.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(73974..76613)
                     /locus_tag="IWO52_000072"
     CDS             complement(73974..76613)
                     /locus_tag="IWO52_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0354372.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(77232..78263)
                     /gene="queA"
                     /locus_tag="IWO52_000073"
     CDS             complement(77232..78263)
                     /gene="queA"
                     /locus_tag="IWO52_000073"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0354373.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            78456..79631
                     /locus_tag="IWO52_000074"
     CDS             78456..79631
                     /locus_tag="IWO52_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0354374.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            79635..80276
                     /locus_tag="IWO52_000075"
     CDS             79635..80276
                     /locus_tag="IWO52_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0354375.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            80273..81190
                     /locus_tag="IWO52_000076"
     CDS             80273..81190
                     /locus_tag="IWO52_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0354376.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            81187..81858
                     /locus_tag="IWO52_000077"
     CDS             81187..81858
                     /locus_tag="IWO52_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0354377.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(82399..82887)
                     /locus_tag="IWO52_000078"
     CDS             complement(82399..82887)
                     /locus_tag="IWO52_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0354378.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(83088..83354)
                     /locus_tag="IWO52_000079"
     CDS             complement(83088..83354)
                     /locus_tag="IWO52_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0354379.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(83354..83617)
                     /locus_tag="IWO52_000080"
     CDS             complement(83354..83617)
                     /locus_tag="IWO52_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0354380.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(83610..83882)
                     /locus_tag="IWO52_000081"
     CDS             complement(83610..83882)
                     /locus_tag="IWO52_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354381.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            84093..84278
                     /locus_tag="IWO52_000082"
     CDS             84093..84278
                     /locus_tag="IWO52_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0354382.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(84420..86243)
                     /locus_tag="IWO52_000083"
     CDS             complement(84420..86243)
                     /locus_tag="IWO52_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0354383.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(86841..89546)
                     /locus_tag="IWO52_000084"
     CDS             complement(86841..89546)
                     /locus_tag="IWO52_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0354384.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(89852..90799)
                     /locus_tag="IWO52_000085"
     CDS             complement(89852..90799)
                     /locus_tag="IWO52_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0354385.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLEASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            90989..91258
                     /gene="rpsN"
                     /locus_tag="IWO52_000086"
     CDS             90989..91258
                     /gene="rpsN"
                     /locus_tag="IWO52_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0354386.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(91434..91796)
                     /locus_tag="IWO52_000087"
     CDS             complement(91434..91796)
                     /locus_tag="IWO52_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0354387.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(91807..92928)
                     /locus_tag="IWO52_000088"
     CDS             complement(91807..92928)
                     /locus_tag="IWO52_000088"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0354388.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(92944..93294)
                     /locus_tag="IWO52_000089"
     CDS             complement(92944..93294)
                     /locus_tag="IWO52_000089"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0354389.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(93424..94935)
                     /locus_tag="IWO52_000090"
     CDS             complement(93424..94935)
                     /locus_tag="IWO52_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0354390.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(95257..96609)
                     /locus_tag="IWO52_000091"
     CDS             complement(95257..96609)
                     /locus_tag="IWO52_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0354391.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(96762..97838)
                     /locus_tag="IWO52_000092"
     CDS             complement(96762..97838)
                     /locus_tag="IWO52_000092"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0354392.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            98034..99779
                     /locus_tag="IWO52_000093"
     CDS             98034..99779
                     /locus_tag="IWO52_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0354393.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(100059..100496)
                     /locus_tag="IWO52_000094"
     CDS             complement(100059..100496)
                     /locus_tag="IWO52_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0354394.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(100480..101853)
                     /locus_tag="IWO52_000095"
     CDS             complement(100480..101853)
                     /locus_tag="IWO52_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354395.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(102175..102663)
                     /locus_tag="IWO52_000096"
     CDS             complement(102175..102663)
                     /locus_tag="IWO52_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0354396.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(102685..103287)
                     /locus_tag="IWO52_000097"
     CDS             complement(102685..103287)
                     /locus_tag="IWO52_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0354397.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        30374 a        21561 c        18223 g        33173 t
ORIGIN      
        1 taagaccatt ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca cgataggtaa
       61 cccaaacatc gttggttcat taatattgaa gacacctggc gcaatcgaca agtttgttac
      121 aaccatatat ggtttgtttt tacgtcctac taaataaata gctaacagta aacctaaagt
      181 tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca cgataaacgg
      241 tgcgctatgt ccagctgcga ttgcattaac gtttgcttca atggctggcg cattgattgt
      301 ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca aagaaccata agaatggtgt
      361 gataaatgca agcagtaagg cagatggata agagtttgca agtcccataa atggttcttg
      421 gaccactgta tagaaagaac ctacgatatc atcaacaccg aatgcagcaa aaattgcagc
      481 aactaaagca aacgcagaga tagtgatcat tgctggtagt aatgaagcaa atgcttttgc
      541 aactgccggt ggcacaccat caggcatttt gatgatcaat ttttcatttc cgcttaaacg
      601 tacaaagatt tctgttgaaa taatcccaac aataatagca ataaacaaac cgtttgagct
      661 catacctaat gctccgccta aagcgaagaa agagcctaaa gaaatcacac ctgcggaaac
      721 accgtctttt cggtagccgt tagctaaatg ataagcaagc aagaaagcaa tcaagacaga
      781 aagaatggca aatgtaccgt tccaagcagc agccccgaat cctttccatg attcaccacc
      841 aaaaattgag ttcatcaatt cttggaaccc gggaatcggt aagttattga tcattactgc
      901 taatgctcct aagatcatta acggcatact caccataaat gagtcccgaa tcgcaactaa
      961 atgccgttgt gcaccaattt ttgatgctac aggtataaaa tgtttctcca taaatgcaac
     1021 aaatttatcc ataaatcttc cctcttttct ttactgtaga ttacgcgttc aatcgacgat
     1081 acagatcaat gatctctttt gctaaatctg taaaagctat agatgtcatc agatgatctt
     1141 gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct tgtgtcagca
     1201 tctctgtttg agagtgatgt gcttggacta aagattcctc tgcatctttg attttttcat
     1261 ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc ttccattgca tcgctttttg
     1321 catttccgcc aaacatgatc agacccatga tggcttctag atttttttga tcttccattt
     1381 attgtatcct ccgataattt ttattatttg tttattagtt tttctgcttg gtcgagtact
     1441 ttttcaccat tcatcatgcc atagtcagcc atattgatta catctaaagg aatgccttta
     1501 ggtgctagct tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc taaaagtaaa
     1561 acgtctacat ttttagattc tagttgatta tccgcatctg aagcagatac agcaaagatg
     1621 tctgcttcca ttccttttgc ttctgctgcc ttttgcattt ttgtgactag gagacttgta
     1681 ctcattccag ctgaacatac aagcatgatt gttttctttt ccattttaaa cacgttcctt
     1741 tctatactta cacttgtata taagcaagta gcgtgccaac tttaaaagcg ctttaaaaag
     1801 ttgatatcat cacatttaag gtctatacca agttgcatac acactaaaaa aagaaagcgc
     1861 acactaaaca cacaaattaa tttttattac cttttttctt ttaacgtgca caaaaaaagt
     1921 tgtgacacaa aacttgtcac aacttttttc aaataaacgg tattcaaaag cttgcatctg
     1981 cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg ctattcttat
     2041 tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa tcagctgacc
     2101 ctgggtatta gctcaaaacg ctggattcac aacctctttt atttcaaata accagtaaat
     2161 acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc aaatccaatt
     2221 ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt ctgcttcgtt
     2281 catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc attggtgaca
     2341 cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct tctgttgctt
     2401 tgatcaagta atgcaaatgc tgtccgataa attcgatacg gtaatcatct tgcacgattc
     2461 ctttctcatc catatactgt tcttcatatt ctcttcccat accgttttct gttatcagcc
     2521 aagggatatt atgataattt tcttttagat acatggctat atcatacatg attttcggat
     2581 agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag acttcataat
     2641 atcgttcagg atggaaaggt gtctcttcat tccattgata acgaggggcg cgaacgcgtt
     2701 taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc gtttctaaat
     2761 cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg aactcttctg
     2821 tgtagtatcc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa tcatacatat
     2881 aagcagcttt ttggtcttct ggagaattgg atcgagcata gaccatttca ggatttaaaa
     2941 tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct ttagccgttg
     3001 ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca tgcggccagc
     3061 gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat gtaaaccagt
     3121 tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca taaaaatcaa
     3181 ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg acttcataat
     3241 gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc atttcactta
     3301 tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa aaacgagccc
     3361 aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc acatcctgct
     3421 ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc caaacaaaat
     3481 gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt cctttccaac
     3541 cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga atagtaattt
     3601 tcatgatttc ctcctgtttt ttagatccca tcattttcca cacgatttgc tacttttaca
     3661 aatggcagat agatcaaaat agaaacaata atacaaacaa gttgcgtaat aactgcaccg
     3721 atatttcctg ctgttgccag ataagcattg acgatcggcg gcattggcca agggaccatt
     3781 acaactgctt tcgccgcaaa tcctaaagcg gttgcaaaat aaccaatcgt tccagtgata
     3841 agaggcgtta agataaacgg aatagctaaa attggattca gcatgatcgg cataccaaaa
     3901 ataactggtt cattgatgtt gaagatccca ggagcaaggg ataacttggt gatttgtttc
     3961 atatcttccc gttttccaac taacaatact gcaatcagta aaccgatcgt gactccagac
     4021 ccgcccatac tcatatacat atcccaaaat ggcatagtaa taatgttcgg tacttctttt
     4081 ccagcttcaa aagcttccgt atttacagca attgccgcta aaagcaacgg ttcccgtatc
     4141 ggcttcacca tttgatttcc atggatacca atcacccaaa agacttgcgc aataaacatc
     4201 aatagcaaaa tacccggtaa cccttgtacg acaccttcta atgggcgttg tacgacattg
     4261 taaatgatat cataaaggta cattcctgta agttctttga tgataaatcc cgcagccgcg
     4321 atacccgata cagtcagaat cgtcgggaat aaagcagaaa aactagcaga aacattcgtt
     4381 ggcacggtat caggcattct cagctgtagt ttcttctgtt tcctcaacca gcaataaagt
     4441 tcaatagaaa ggatggagac caacattccc aaaaataacc ctttcgtatc tgtatattgt
     4501 ttagccaata cgttttctac tataacagtc tgattttcat tcactaattc ataaacagtt
     4561 ggattaacag ataaataaga catcactgcg atgataccag ggaatacacc atccacttta
     4621 ttcagcttgg ctaattcgat ccctatcaga aaaacagcat agatggtcaa gaaacttagt
     4681 gtgacatagc tgatggcaga tgcgataggc tttaattctt ctaaaaaacg caagaaactt
     4741 atctgtgcta agccattttc cgagtcaaag accattgctg aaaacaatgt gccaaaagca
     4801 cccgtaataa ttaccggaat caatgcagca aatgcgtttt ttatcaccat gatatacctt
     4861 aaattattga ttctcgttgc cacgttccct aaaacattgg ctaatttatc cataaaactc
     4921 attccagatc ccctcattcc tgcttcttct ccttgacgtc atctcttttt gtctattagt
     4981 cagcgcttac atttcgatga acagaaacta tttactcctc ttttttacca aatataaaac
     5041 aaaaattcat ctgatgtatt tatcataaaa tacatcagat gaactgtcaa ttagtttctt
     5101 tttctcctat tattttctgt aattctctcc gatttattgc catatggaca gtcattgcat
     5161 caaacgcagc aagtgcattc cgcctttcga tggcctgaac gatctctgta tgaatatcga
     5221 tcgtctcgat tttcagatgc tggatatcat aatttttatt aaacaaagca attgattcat
     5281 taatgattgg gataatatgc agcaaggcta cgtttccgct agcttctgca atcatcgtat
     5341 gaaaatcgat gtctaattga acatgttctt gtgtgccttc tcgaaaagaa gtttcaatct
     5401 tttgcgttaa ttttttcatt tggacgattt gattcttcgt tgcatattcc gctgccaaac
     5461 ctgccatctt aggctccaat aaatatctca gttcaaataa gtctcgtatc atccgatcct
     5521 gatcttcaat caaagaaaaa ccgagtgggt cattggcaat ccctcgctta tcactgacaa
     5581 atgtccccgc tccttgccga acttcaagga tattcctgga agctagagcc cgcacagctt
     5641 ctcgcaccgt gcttcgcccc acctctaaat aatcggacaa ttcatattca ttcggcagtt
     5701 tatctcctac ttgcaactgc atttccttta aatatctaag gatcttgtca gtagttactt
     5761 ctactagggt tttattttct tccaaaccac atacctcctt ttcatctgat gtattttttc
     5821 gtaattatta taacacgttt gaatcatctg atgatttttt gtatacaaaa ttgattaatc
     5881 cgttcataga catacaaacg aagaagatat ttaccataaa aaagactagg ataaaattca
     5941 ctcctagcct tcgatcattt ttattttgaa cgacaaaaaa tatgatgttc aataacagac
     6001 gatcttcatt cgattcttga tgtcatcttt tgtttttttt aatatatgga taaaaagttt
     6061 gattacatcg tttctattct agaatttggt cgattctcaa ttcttcttct ggtgagaaat
     6121 ctaaattatc catcattcga acactttctt gtaactgact taaccgactt gctccgacca
     6181 gtacacttgt aacagatttt tgtcgcaaat tccatgcaag ggccatttgc gctaatgatt
     6241 gtcctcgtga tacggcgatt gtttgcagtg ctttgatttt ttccagagtt gatccaactt
     6301 gttcttctga aaggaatggt atttccttac gatgtgctct agaatcttca ggaataccat
     6361 gtagataacg atttgtcagc aacccttgag caagcggact gaacgtgatt gcccctagcc
     6421 cttcttcttc taatactgga gatagatcgt cttctagtag tgcacgagaa aacatattgt
     6481 agcgcatctg atgaataatg aacggtgctt cctttctttt taaaatttca gtcatttttt
     6541 ttgtatcttc tccattatag ttggatatac caatatataa tgcttttcct tgtcttacaa
     6601 gttgcatcaa agcttctgct gtttcttcca aaggtgtatc agggtctggg cgatgatgat
     6661 agaagatgtc aacataatct agccccattc tttggagact ttggtcacaa cttgaaatga
     6721 tggatttacg agagccccat tccccatatg gcccttccca catatgataa cctgctttgc
     6781 tagaaataat cagttcatca cggtaggctt gaaaatcttc tctaaaaata cgcccaaagt
     6841 ttttctctgc agctccagca gggcctccat aattattcgc aaggtcgaaa tgggtaattc
     6901 ccatgtcgaa tgcgcctctt aaaatctctc tttgattatg gataggatca taatcaccaa
     6961 aattttgcca taatccgaga gataaaatcg gtaatttcaa acctgaatga ccggatttac
     7021 gatattccat tttttcataa cgtgcactat ctgcaatata caatttttct cctcctcatc
     7081 aaatcttttt aaaacgcttt cttttttcta tttttagttt actacatttt aaagaaaagg
     7141 agttaatcaa aatgattttt tcgtatattt ttttgccatt tgttttttac tcgttgatag
     7201 atcagaaatt gattacgtgc ttgctggatt tttttataac gtttcggttg ttttttgtaa
     7261 aattgctgat gatagttttc tgctggccaa aacgtaccgg ctggttcgat ttttgtgacg
     7321 attggttggt ggaaagttcc tgaactgtct agtttctgtt ttgattgttc cgctaattct
     7381 ttttgtcgtt cgacttgata aaaaatcact ggacgatatt gcttccctct atcttgaaat
     7441 tgtccaaatg tatctgtggg atcagtaatt tgccaataga ttgtcagcaa ctcgctataa
     7501 cttatttctc ttgtgtcata aatgatctcc acagcttcca catgacctgt gtaaccacca
     7561 cttacttgat cataatttgg tttttcaaca tgtccgcccg tgtatcctga caaaacagat
     7621 aggatccctt ttttcgattc aaacggctcg accatacacc agaaacatcc ccctgcaaat
     7681 actgcacgtt cttgatagtc agcatcttgc atggcatgct tggaaaagtc atatttttgc
     7741 tccttatcaa atccttccgt aatcaataaa taaaagtcca ttacatcagg cgtcagattc
     7801 attcggatag ctaatggacg tagtgttgct tctagttttg ataactgttc tttaacggat
     7861 atattttctc ttatgtcatt ctttgtctga atcaatactt ttcgttccca atctcgtgta
     7921 ttcggattca aaatcagatt ataaagtgtt tgaatgaata tttgttcctc atccatagat
     7981 tctcctcctt tccgaagaag acaagctagt gactttagct gtaatttatc ataaagtgag
     8041 gaaataagcg attacttcat ccccctacag atttttcgta taaaaaagag agaatcctta
     8101 tcgacagatt ctccttttta atcaatttat tttataaatg aaaagttaat ttacctttta
     8161 tggaaaacta ttttaaaaaa taaacataaa tatgcagtta atgtaacaag ataagaaata
     8221 atagtcgtta ctgtataact tgtgcttact ttaatatcag caactccttt gactccgctt
     8281 atttgtacca atccattagc attcacctgc tcctttacag gttgatgatt tatttctaca
     8341 ttcgttccta aataagcata tacaggaagt gtgattactt ggctcttctc attagttact
     8401 ctatatatga tagaactacc agttgtttta taaggaaggt ttacccattg actgccaatc
     8461 tttgcttcgt gttttattat atgctcctta ttctccaatg aagcgatagg agaataatca
     8521 tataaaaaat aatccattcc tttagtggta tacgcctcta ctgtttcttt gtttacaaca
     8581 atatgtcccg gctgagaaat tattttttga ttgaaatttt ttttactaga aacagttaaa
     8641 gtgattaata acaaaacagt cataaataaa taaaatagtt ctcttgtctt attgtatctt
     8701 ctatttttga aaaatatcat cgttaatatc agagaagaga atattatttg caagcttaag
     8761 atccgccaag gaaattgtaa caatttggct ggcgtatttt gcagaagata ccatggcacc
     8821 aaactagttg tagctagcca agtcaaacac gtaccataat aaatcttttt ttctatgttt
     8881 gttaattttt taaataaaat caggggaata cataaagata acagaccaac tagtcccatt
     8941 gagtaagtag ttaagtcgtt gttcatagct tcagtcagac tatcgaacac attagatgca
     9001 tgagcatata aatttgtcac gctaggtttg ttgatttttt ggtagagcat ttgttcaaac
     9061 atcggatacc aaaaagcact cgtcagaacc aatgtcattc ctgccgcact aaataatttt
     9121 ttaaatatag caataaaatc tgcattcttg aaacctttag aaaccaaacg aataaaaata
     9181 aaaatgataa ttaatgctgt actcattaat gttgatagta catggctgta cactagcaaa
     9241 ctcatactca atgctagctt tacagacgga tacttttctt cttttccaga gatgatttga
     9301 taagcataaa ataatacagg gataacaagt gttattgcga tagcttcggc aattgcggaa
     9361 cgatagtaga tgtctactgt ccgataagca gagaaaatat agagacaact aaataaaaat
     9421 gccgcttttt tttgttttaa aaaccgctcg ccataatgat aagctacgag tgttgtacac
     9481 aagttcaata accaaacgta aataatatag cttacaatca aattttttgt catccaatat
     9541 aacattacag ctggaaaaaa agtcagaaag ggataaaaat aatttacacc atatccagta
     9601 taattgaatg tctcataatt aatcggactg gtaaaaatag tcgataaccc cttgattcga
     9661 gataagtgaa aagaaagatc tccagtggta taaatactct gtgtattttt gaaaaagatg
     9721 aaagaatata tactactaac tataattaat ccactcaata ttactacttt tttatttatt
     9781 ttcattcttc ttgtttatct cctttttagt cacatatcga gaatctttca acttattagg
     9841 tgcaaatcgt ttaaaacttg aatacaccac taaacaataa gtcagaatac tgatccataa
     9901 agccatgaga gcagttggag tatattttgt tgtaatttct atcttatttt ctccagcatt
     9961 tacatccaca ttcactaagc catgtttttt ataagagtca tttacgactt taccgtttac
    10021 ttttacctgt gtacctaaat aagcatatac tggtactgtc actttttgct ttttgttgct
    10081 tgccactgtg taagtaatgt tattatctgc agctttatac ttcccttttt cccacgattc
    10141 tcctactttt acttcatgtt tttctaagtg aggcctttct tttatcgctt ctgttggggc
    10201 gtaatcgtat aatcctccca tatctgatgt tgtataacgt gggagtgttt cttcattgat
    10261 aacaatatga ccgtgttttg attggataac ttttgcgtaa ttttcttttg ctgctataga
    10321 cgtgacgagt agaaacacca cagtagcgcc aatgattaac catgaatatt ttttattcat
    10381 agtctttttc ataaaaacaa gcgttatgat cattgaagaa aaaaagattt ggatacctaa
    10441 gatacgccaa ggaaactgga gtaagcttat cggtgttttt tgaaacaaaa accatggaaa
    10501 taaattagtt acgcataccc atgtagaaac tacgccaaca tagatgattt tttctttttt
    10561 ctccatttct ttcagtatga ataaaggaaa aatcaacgca accaatccta ataaccccat
    10621 agtgtatgtt gtaatatcgt tatttaacgc ttgaatgata ctatctgaaa tagaaagtgc
    10681 ttctagctgc aaatctgttt tgaagggact gtgtatttct tgataattca tctgctgcaa
    10741 cataggatac caaaaatagc tagttaatgc acaagtcaaa aaaacagcct ttactaaatt
    10801 cacgaccaca aattttatat cgttttttat ttgcttgctt aaaaacaaac gaactataat
    10861 catacaagca atcaatgcac tacacaatag tgtagttaag acatgagtat acacaagtag
    10921 acttatactg acagataaag cgatgacttt tttttctcta ttgaatatca atccataagc
    10981 ataatataaa acagctggga taatcgtaag agcgattgct tctgcaattg cggatcgata
    11041 atagatatca actgttcgat aagttgcaaa agtatataaa caactaaata aaaaagcagc
    11101 atctatcttt ttaaaaaact tttctccata ataaaaacta atcagaatcg tacaaatatt
    11161 taataaccaa atatatagaa cataacttaa tattaagtta tttgaaattc cataaaaaac
    11221 gactgcagga attattgtaa gaaaaggata aaaatagttc agtccatctc cataattgtt
    11281 aaatgttgta tagttgattg gcccttcaaa aatagaagat aaccctttga ttcgtgaaag
    11341 atgaaaagat aaatcacctg ttgtataaat ttcttgagca tttctcataa aatttactga
    11401 atataaagta ctaagtaaga taagaactac taaaagaaat gcttttttac ttattttcat
    11461 agatttgttg ttctcctttt ttaattctat tttcagtata ctagaattgt ctttttttaa
    11521 taagttttgc gttaaatttt tcatagcact agtatgatat actagaaaag aatcaatttt
    11581 tattactttt aaataggaga aaaaaatgaa aaaaaacaaa aaccaaataa tagatatcat
    11641 ttttatgtta tttctaagtt ttgcttacgt catccctcta ttcatgtcta agggaatcta
    11701 tcatagtgtc aatcaggata cttactttca tttatctcga atcataggac tagataatgt
    11761 atggagttct ccagtaaact tcaataactt cgatcatcat ggaacaatga tgaatatttt
    11821 ttatccatgg cttactcttt atccagcttt tttattttat aaaatgatgg gaaatcttgt
    11881 tttaagttat aatatctact atttttttat cactttttta acaatggtcg tttcttattt
    11941 ttctatgaaa caaataaaaa acaatcgtta tatttcgctg ttattttcga ttatttatac
    12001 attttccgcc tatcgtgcca ttgacatatt ccatcgtgct tcattgggag aagctgtcgc
    12061 tttaacattt ttgcctttga tactaatggg gtgttacgaa atttatatta gagattacca
    12121 aaaatggtac tggttgtcta ttggcatgac gctagtagtg tatactcatt tactttctgt
    12181 cgctatggtt tctgtattta ttggaggaac gttgtttctt tctttttatt tttgggacca
    12241 aaaaatcgct agactcttaa gtcttttaaa agctaccgtc ctcacctttt ttttaagtgc
    12301 gggattctta attcctttta ttcaacaatc tagagcacaa gaattgaaag tcccactggg
    12361 aaaagaatta agtggtatgg ctcctagtga tatgctgaca catattttaa ataataacta
    12421 taacaactat actattggat tattcttatt tctcggattg attggtgctt ttatttttat
    12481 aaaaaaatta actgctgatg atttatttat tttctttctt ggtgtatttg ttcttttttg
    12541 ttcaaccaat ttatttcctt ggcaactttt taaccataca ccagttaaga gtctacaatt
    12601 tgtctggcgg ttaaatggtt tttcttcttt atttatagcc tatacgatga gtatagtgat
    12661 ctattatatt ttttctacta aaaataattc atggaaaata atggtcttta cgtttcttgc
    12721 actaatattg catctctcag gagtaagcaa ttctatccat gctaacaaag attcattaac
    12781 acttatagca cctgaagacg ctgttgctat agctgaaaat tacaatcata ctgattatgc
    12841 aaacaaagag tcaatctatc atcctgatat gattaataat gatcaatatt tactaggtaa
    12901 ccaggaaata aatccaacaa ctcattttat gagtaacaaa cttacaattg aattagataa
    12961 ttcgaataat aaaaatactg tcttgacgac acctatttat cgttataaag ggcaagtggc
    13021 ttctattaat ggcaaactag tacaaacaaa attatctaag tttggcacaa ctgaattaac
    13081 tattcctcca ggtataaata aagtcgttat tacttatcag tacacaaaac tagcaattgc
    13141 ttcaagatat ttatctatcg ttactttgat tttattttta ctttatcgat ttactttttc
    13201 taaacaaaaa caaccgagaa gagaagtaca acattctcac tagattttta tttaagaaag
    13261 actttttagc tgtacacata aacaggctaa aaagtctttt tttatatttc tttaatttgc
    13321 ttcatacatt atttaacatc gatatttata ctgtgaaaca tgcgtttcat ccattatcaa
    13381 agtattttta tcgataataa acttcatcca aaaacagacc ttgtgcaggg acggtttctc
    13441 ctgcatgttg ccgcactttg ctttcaaata tttcatcgat tgtttctatc ggcatggcac
    13501 ctgtgccgat ttctagtaat gtccccatga taatccgcac cattttatat aagaatccgt
    13561 ctccgacaaa agtaaaatga agcatttctc cctttcgttc gatagataat tcatcaatcg
    13621 tacgcactgt ggattttttt gattttttca atgcagaaaa accaatgaaa tcatgtgtac
    13681 ccactaattt ttcgcaagct gcattcagct tgtccatatc caactcttga gggaaataaa
    13741 agctgtggtt tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa tagctgtatt
    13801 ttttgcccac cgcattatat cttgaatgga agcgctcagg catttcttcg attcttttga
    13861 tgacaatatc ccgcggaagg taacgattca taaagtcgat catttctgcc agtccaattt
    13921 ctgtgtttgt tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca tctgttcttc
    13981 ctgatccaat aatttctatc tcttcatttg tcatctgatg caatactttt tcgatttttc
    14041 cttgaatggt tttatcgtca tctccaagtc tttgccatcc tgaatatctt tttccgtcat
    14101 attcgattgt caacttgatg tttctcatat tttagtggct cctctgtctt ccatttggtc
    14161 ttctctctac ctttttaaga ttgtagcaca aaatatagct ccttgcttct ctaagacaga
    14221 agctatacaa gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca gccaccggat
    14281 gaagtatccg ctgactgttc aatttgacgg ctcaagtttt actgtttgag atgcaagata
    14341 ctcaaccagg caatgggcac ataattaaag aaaaaactga ttctttggca aaaacctatt
    14401 ttattcagta atggccaaga atttatagaa ggaattcttg ccagtccata aatcgccgca
    14461 aaaagcaaac tgatcacaga cagtccaagg tataagtggc ttacgtccat ttgcccattt
    14521 tttcgataga tcaagaaaag aagaactgga aacaataaaa aaccactgta acctaaacct
    14581 gatccgatat tatggaccca tgtcgagaca ttccacgaag attcatgggt attgatactg
    14641 aacaaacctg taaagataca atcacctata ccatagaacg cgatggctgc tgtaactagg
    14701 gtagctaaca cacgagatgt actaacaaat gcagcataca atgcaggcaa tgacaggatg
    14761 aaaaagatcc ctgaaaccac agaccaaata agaaacgctt ttcttacagg actggaaacc
    14821 tctccaaata cactgatcac gctcttcatt tgatctaatt caggataata caatcctaat
    14881 acgtacggag tcaaaaaatc actcagcacg cctaataata aaaagaaaaa accaaattta
    14941 cgtaaaaaat ccatgatacg cctccaataa caattaccta tgcttttaat tgttattata
    15001 aacgacttta tcttgattta acagaaacct ctatttacac gaacatctat ataactcatt
    15061 aaaaactcga atcattttaa aaatactaaa tatctaacgt tcaacgttca cttaatccat
    15121 ctaaacaatc tttcaatttg attttttccc cacttctttt acgcgcgcct agctctccta
    15181 ttgcagtaaa caacagatca cttgacgaat ttaaggcagt ttccattgaa tcctggataa
    15241 caccgataat aaatccaata ccgacgattt gcatcgcgac atcattagag ataccaaaca
    15301 aactacatgc taatggaatc aataacagtg aaccgcccgc aacacctgaa gctccacaag
    15361 cagctaaagc agacaaaata cataaaagga ctttcaaaac aaaaggtgct tcaatcccta
    15421 aagaatgtac agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg cccatattga
    15481 tcgtagcacc taacgggatc gatatggaat aagactcttt tgtcaatttc aatctttcag
    15541 ccaacatcat gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa gcgggaagtg
    15601 cgctttcttt caaacaaaag aaaatcaatg gatagggatt ttctttaata aacaaaaacg
    15661 tgaggaatgg gtacacaact aatgcgacaa acagcatcgt accgatcaaa acaagtaaaa
    15721 gctgcagata ctctgccaat ccagcgattc cagtcgtagc aattgattga taaaccaatc
    15781 ctaaaatccc tatcggagca aatgcaataa tcacttgtac gacttgtgac aacgctatag
    15841 atatctgatc caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct agtcccaaca
    15901 atacagccca gaataagacg ccaaggtaat tcgcatcaat aagagattga acaggatttt
    15961 gtgtgacatt cgtcaacata gtagataaaa cactttccaa actttgcggt gctttttcag
    16021 atactgtttc ttgtaaagta ataggaacag taaaaattga acttacaata actgctacaa
    16081 gtgcagaaag caatgttccc actaaatata agatcaaaat aggcttcacg aatgtcttat
    16141 tcccaatttt atgcttggca attgacgcca tcgtcaagaa aaaaaccaga ataggtgcga
    16201 ttgccttcaa gcaactgaca aacaacgttc ccaatattga aataaatgtc catgtgggga
    16261 aaaagatacc aaacaatatt ccgactaaaa taccaacaac aatacgttga actaacgaca
    16321 tatccaaaaa cttctctaac aaatgaccac cctttcacaa ttttaacaat cattgtatca
    16381 ttttatgcaa agaaactaaa ggtataattt tctaaaaatt ctaacaatac aaacaaccat
    16441 ttcttgctaa ttttgtactt tttctgtcta cacgttgttt tttgagatat tctaagttag
    16501 gatgttatta atgggcaact tatcaggaaa cgttttctta tgtttttgtc caattttttt
    16561 gtgaggtgtt tattcttgaa aaaaataaag aaggagagtt atggcttcct gacaatcttt
    16621 gtcgcttgta taatgatggt tttacagcgt atattgattt cagcagtcac tattcctaca
    16681 tatgcaacaa accctggtcg attctacatc tatacatttt tacaaacttc cgttgttatt
    16741 ggtgcaaatt tgatcccact ttacatgggt taccaatatc aaaaaatcgc ccgtttaaaa
    16801 gttttacatt atttaagtcg ttttgccttt atattcctaa tagccacact agtttgcaat
    16861 atcttttttt acgtacaagc tggcagcttg aatattcgtg attattggct tatcctaaca
    16921 cctatcagtc agaattactt tacttatgct gtttcttgta ctttgctttt ttgtagtatt
    16981 ccttatacag ttggattttt aaataaatta tcgaaaacaa caattaaata tctactatta
    17041 tttttaacaa ttggattagt tggctgctct actctattta ataaagaccc gtttgcatta
    17101 cagaatggac agagtatttt atggattttt tatttatttt taatgggtta tggtttgaaa
    17161 gaatggaact ggaaagaaaa ggtccgctat aagcttcctc aagtatttat cagcttgatt
    17221 gttttattcg gtttgattat tttgatgaca caaatttcac tcatcatcag aggaaataca
    17281 gatacagcgc tgaggttttc taaaccatat tctctcttcg caatgtatta tactttctct
    17341 tcgtttttat ttttagaaat gctatctgat aaattaggta taaaaggaag aggaaatttt
    17401 cttgctgtat ttttgattgt cactcaagtt atatgtaatt tccctttagt ttcttatcgc
    17461 gtatctacat ttatgaaacg tgaatttcca aactcaggga aggcatggct gataaatatc
    17521 gggcagtttt ttatttgata tttattagca gtgtccctcc tagttatctt tttattttat
    17581 atacaaaaaa tccgtttcat tcatcgtttc atcgaatact tcgcttttga ttcgattgaa
    17641 caattaactc agcgttggaa aaaaagaatt cattggcttt ttgttcataa aagttatttt
    17701 ttagttgcag cattttttta ctgctttacc ttcgtgcaga ttttcgtttt agaaccaaaa
    17761 gaaggttgga aagaaactat ccaggttgcc ttaaaaatat ttactcagcg acaagcaccc
    17821 atgatcctga caatcatcat tattatggga tttttcttct tactattgtt gatttccaat
    17881 cgtttttggt acgccttgac tgctaccttg atcattaact tactgctcac tatttcgaca
    17941 gtaatcaaaa tggaaatgcg ggaagagcct gtctttcctt cagatttaaa aatgctgacc
    18001 ggattgtcag aactattatc gatggttagc ccagtcttat taatcgtggg cggattgatc
    18061 cttctcctat tattgattac tagtattatt gtacaacgta gactacaaaa acagtattcc
    18121 ttgaagattc attggaaacg tcgatttatc agtatcgctg tattattagg atgtttttcg
    18181 ggtgtctttt tcattaatca caaaaattcg ccttctttct tgctgtttaa tttatttaaa
    18241 gtaaacaaga ctttctttaa ccagcaaggc gccgtaaaag ataacggacc agtcattcaa
    18301 ttcctaaaca atatcgacat taaaatcatg gaagaacctg ctggctattc gaaagaaaaa
    18361 attaatggaa taatgaaaaa gtacaacaca aaggcagatg aaatcaacca aacaagaagt
    18421 gactggttaa acaatcagac gatcgttctc aatttaagtg aaagcttcag tgatcctgca
    18481 cgtgtcccta atctgacagt acctacagac cctattcctt acatcaaaag cattataaca
    18541 gaaggaacag gaggattgat gttatccgtc ggttatggtg gcggaacagc taacatggaa
    18601 tgggaaggac tgacaggctt gagtatcagt aacctgtctg cttcgttagt tacaccttat
    18661 acccagctag tggagcgtca gaaaataagt ccaaatatta ccgatctatt cgatgaaaaa
    18721 atagccatcc atccttttac agctgctctt tatcgcagaa aacaagtgtt tgataaattt
    18781 ggttttgaca ggttctatta tttagacagt ccggataaat taacttatac ggataaaata
    18841 gaacaaagcc cacgcatatc ggatgattca gcatacaaag aaaccttgaa agctttacaa
    18901 agcaatacgg aaacgtcaca attcatccaa ttatccacta tgcaaaacca tatgccttat
    18961 gatgattact atagtgaatt gaactacact gctgaaggaa ctgctgtcat tcctaataga
    19021 agaaaagaat tacaaactta tatgcaaggc cttcactata ccgatacagc agtaaaaaaa
    19081 ttcatccaag aaatcgacaa aatcgataaa ccgattacct ttgtttttta tggtgaccat
    19141 ttacccgcaa tctatagtgg gaatgattcg aaaaaatatg gattagaaca gcaccagaca
    19201 gattatttca tttataacaa caagtatagt cgtgaacaag cagctacgtt aaataaagaa
    19261 gttgttgctc ctaataactt tggtgctatg gctcttgagc atgcgaatat caaagtaact
    19321 ccttactatg cgttgttgac agaagtatct aatcatctac ctgcattaac aattgatcct
    19381 acagaaagct tgtctaatcg ttttaatgga aaacaggtct ttgttacaga ggatagtcaa
    19441 attatccaag aaaaaaattt aactgagaaa caaaaagaaa ttctagaaga ttatcgtctg
    19501 atacaatatg atcttgttgc aggtaaccag tacagtgcaa catgggcgac acaaaaaata
    19561 aaagactaac aaataaactg agcaaggggt tgtgataaaa gacatgtcac aaccccttgt
    19621 ttatctcatt aatttgcttg tccataaacg atttgattaa agccattttt caattcatta
    19681 ttcgctgtct cgttgaagta aagataatta tcgggattcc cctgattttg ttgtgtcacc
    19741 aatacttttg gctgattttg ctgaaacccg aatagataga cgtgcttttt cagatatggt
    19801 tctgtctctg catcggaata gacggctgtg atttgatagt ctgcttgacc tgtcccactt
    19861 tctgaccatt ctgcagaaac aggcactcca ccaatagcca tcttccactc cccgttcaaa
    19921 atagcttgag ggactttaag accatataag tccacactca tttgattcgt ataacttttg
    19981 tattgttgtc ccattgtttg gccccatttg gtcataaatg cctctaattg tgcggatttg
    20041 tctgcattcc acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc
    20101 tctacagaag aacttgaagt agatgtttga gttgtttcag tcgttttgga agactctgac
    20161 gaagtagagg tatctgctgt ttctgtactt gctgcagttg acttgctctc tgtgctagtt
    20221 gttgtagtag ttgcaactgt tgctttcgct gtttcttttg ttgattcttt tttcgattca
    20281 ttcccgttgg tacaaccagc taacaaaaaa gctagaccaa ttgttactaa aatccttttt
    20341 ttcaattaaa aaactccttt cgattactcc cacttcttta ttctatcaaa taagcaatgt
    20401 gattttattg atttttcgta ttttctcatc aatctttcaa atcttctaaa aactgagaag
    20461 cacccgctga tgttgtctgt aatacatcag cgggtgcttc ttttatttta atcttcgtga
    20521 ttttccaatt tacctcacgc tttatatttg tatttgaaga aacatagact tttagtcatc
    20581 atcgtcttcc tcatccaaat cttgtcggac agtttttcct tggagtcttt gtacttggta
    20641 gatgatttct ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc catatttttc
    20701 ttctttttca gctaatttga ttgcccgtgt gataaagaca ttttggaaat caaaatcttt
    20761 tgcaaagttt tccaacattg ttgaggctgg taaatatttg ttttgaccat gttcgtaaat
    20821 ttttccataa cgcatgactt cttctaaagt agacggaacg atttcatttt caatcactaa
    20881 ctcatgagag attttattga accatctcgc ttcttgatca gcatattctg aaaaaagggt
    20941 acttaattct tgcgaagatg tacccaaaac atacaattgt gcttggatca atttcatttc
    21001 ttctaattta aaatttgcta atacatgccc caccatggcg ccagcagttg gtttatgatg
    21061 gtcgatatct gcttgttttg tttcttgaac aaatttttct tccactatat tcattgattt
    21121 acgcctcttt tacttttgct gattggactt cataacctaa tttttccaca actgcagcta
    21181 attgttcagg gtggacttca tctggatcaa agtttgcttt gattttgctc gcattaaaca
    21241 aaactttcgt attgcttacc ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac
    21301 agcttggaca agctagtgta tctaattgca taataatttt tttcatcgta gttcttcctt
    21361 cctttttctt gatagtctta ttctagatta cagcttccat aaaaaaattg attcttatca
    21421 agttttacga tttttcttct ttgctttcgt gtccaaaata caataaacgc atcgcgttaa
    21481 agataacgat caagatacta gcttcgtgaa cgaacatccc actagccatg taaataaacc
    21541 cggcaaataa gccgttgagt aaaaataaga cgacaccaat ggcaatcact acattttcac
    21601 gggtatttaa aacagttttc ttagccaagc ggtaggcatg aaccaacgat tcaaaattag
    21661 attgcatcaa gacaacgtca ctcgtttcga tagcaacatc cgttcctgat cccatagcaa
    21721 tcccgatatc agccgcagcc aaagagggag aatcattgat tccatctcca ataaaagcaa
    21781 cgcgcaatcc ttgttcttgg tattttttca caaattgaac cttttcgtct ggtaataatt
    21841 cagcatggac ttcatcaatg cctaacatat cagctacata atcagcagtc atctgattat
    21901 cacctgtcag catcactaac tgttttgctc ctgcttgttt caacttagct aattgttcag
    21961 cgacttctgg tcgaatcaca tctgacacac ctatgatttg agtgattttt ttgtcgattg
    22021 ctacaataat gaccgagctg cctgtttgtt gtaattgatg taaatcttgt acttgctttg
    22081 gtgtgagaga aagtttgtga gcggcaatca atttggtgtt gcccgcacaa actgtatgac
    22141 catcaatctc agctgtgatt ccttgtccct tgattgtctg attatctttg actgctaatt
    22201 gttgaaaatc aatgtctttc tcctgagcat aatggatcac ggcgcgcccc agaggatgat
    22261 cggataacgt ttctaatcga gccactaaag ctaagctgtc cccttcatcc tctgtatagt
    22321 tcttgatagt ggatacagca gtagttcctt tggtcaatgt accagtttta tcaaagacaa
    22381 aagtatcgat tttggaaaaa gtatccattg cttcaccgcc tttgactaag atgccccgtt
    22441 tcgctccatt accgattcct gctacattcg aaacaggcgc accaatgact aaagcgccag
    22501 gacaaccaag aaccaaaaca gtgattgcta aacgaaaatc acgactaatc agaccaacaa
    22561 ccaacgcaat aagtaaaacg gttggagtat aatactgaga aaatcgatca atgaattttt
    22621 ccgcatggga tttggtatct tgtgcttctt ctactaattc gataattttg gcaaaggtcg
    22681 tatcatctcc gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa atcgttccag
    22741 agaagacttc atctccactg attttgacga cttgtcgtga ttcaccagtc actgcggctt
    22801 catccgcata ccctttcccg tcaatgactc gtccgtccac aggaatagct ccaccagttt
    22861 ttactaagac gatatcgcct tcttccacgt catcgatttc cacttcttca gtcgttccat
    22921 cttcttggac aacactagca gtagatgggg ccatctcggt caaagccttg attgactccc
    22981 gcgttttggt caatgttttt tgttccagat agctaccaaa caaaaacaag aatgtaacga
    23041 tagctgactc cttgtattct ccgatcataa aggcaccaag cacagaaata gtaacgagta
    23101 aatcaattga gataactttg actttcactg cctgatacgc atggatcata ataggtatga
    23161 ccgcaataat agaagctatg gctagagcaa tgttatagaa caagacaaaa ccagtaaatt
    23221 ttccgataaa accaaaaata atcaacacag ctgataaggc ggtgatatga tttcgatgct
    23281 cttggatcca cttttgtaat ttcatctttc tccctctttt cctttcattc ttacgagact
    23341 aagtatagat acttatccat cataaaaaat tgaccactat caagtttagt caaaaaggcg
    23401 gttttctttt ggtagaatac actacaaaaa atagttagca agaagactca ctaaaagtga
    23461 tcttgccaac tattgtcgtt tctatcgaaa ttttcattga atgattttcc tgtacagcat
    23521 acatcggaga tctatttctt tatctaagat ttttatcttg ctaatttttt cctttagtct
    23581 ttcaaatcca cgtcgttcat agaattgata cgtacagcca ctatctgtaa aaaggaatat
    23641 ttcttttcct ttctctcttt tttcaaattc tgttagcaac ttggtaccga tgccttttgt
    23701 tttcaaatca ggatttgcag ctaaaaaaat gatctctcca tctggcgaat gcttctttaa
    23761 atatgctgca agcatttgtt catttgctgt ctcgtaagct cctttacttt ctttagcaaa
    23821 tatattttgt aaaaaatcga atatcttaac atatatttgc tgtccaaaag aacgatgttt
    23881 ctttttttct ccttttatct ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa
    23941 agcaagaatt tgagttgctc gattcaactc catatacaaa aaatatcttc cgtaaagatt
    24001 cagcagaagt tcattttcca tatatctctc aaagttcatt ccttcaacag caaaatagat
    24061 agcttttttg taatctttct tttctaaggg tttaaaagtt agtttcatca tttcgcctcc
    24121 tcaacttcaa tgattgtgtt atacttacat tataatagac acagtgtcta ctatctataa
    24181 tcaatcatct ataagtttgt cgattaagcg acagctttgc tttgatatgt cggataagga
    24241 gataggtaaa tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa
    24301 acactatcca cattattagt aaataaaaag attcaagcaa tcactatacg cgaactaaca
    24361 gaaacggcag atgtacatcg ttcgactttt tatacacatt ataaggatat ctatgatcta
    24421 tatgatcaat tagaaagcaa cttttttcgg gatctaaatg aaattctctc ttatgatcca
    24481 gcccatagct atgaggaact atacactcgc ttgattgact atgtttacgc aaatactttt
    24541 ttatatcgct tatttatcct caaccatggt gatatttcgt ttcagaagaa agttaaacaa
    24601 gttattgaag aaaactatct gaacatatgg ctatatgaag acaagaaaac gattatcaca
    24661 gaagaaatga gattctttac tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg
    24721 tgggtagaaa gcaattacaa gtactccaaa aaagaattat tagaactctt acagaaactg
    24781 aatgatcaca tagagaaaat catgccttga ataaaaacat actaagctta gttaagaaga
    24841 accgtaacct tctcgtataa caactgaatc ttatttagcc aaattgttta ctgaagattt
    24901 tttgagtaaa atttctataa ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc
    24961 ctctctaagt gatatatctt gttgtaccaa tcaaattcac ctcatcatta ataaattact
    25021 tttaggctaa tttattacaa cttataggtt gaagctgtaa ccaatttata gtcatattat
    25081 atttttatca tccagtttat tgaattcttc tcgtttacct caattcattt tcaaggggaa
    25141 tcagctcatc atctaacatt ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg
    25201 gtttcgcttc tggatgtttg gctttatatc cttcgtaatc cttactgttg ataattgctt
    25261 tcatattagg aattggatct tcacctggca tatattgcag attagaagca tgtaattttt
    25321 tctcagaaac aaaccactca ttggaaaaga catgaatcaa atcatcactt acttgtgtaa
    25381 aatagcgacg tttaatgaca aagatatctt cattgctatc tagtgtacct tcatttcttt
    25441 gtgcttaaat tcaaagataa ttttctcaat aaagattttt gattccagta agattccttt
    25501 actcctttta caacatagcg ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca
    25561 ttaaaaatag cccaattctc tataaagaag attaagcttc attattataa tataaagttt
    25621 aaatttggtt tattacttac tttttcttat tctctagtaa gctaatattg aaaaaaatgt
    25681 aatcagatca ttatttttat atgttgtaac ttcctgataa gaaaactaat taaaagaaag
    25741 aggtaactac ataaactaat taaaaaagct aacttcatag ttggaggata aatggaagaa
    25801 acttcaatga ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc
    25861 tcttttttgt ccatttactt ttgcgactgt tccccaataa tcaaatacag gaagcgttat
    25921 tttttgtcct ttagattcat gtactgtata gactattttc gaatccatca cttgataagg
    25981 catagtatgc cattccttgc caattcttac ttcatgattc tgcaaatgtt ctttgtattg
    26041 taatgcttcc gtgggtgcat agtcataaag tcctccggga aggagtgtcg tgtaacgaat
    26101 aagattttct tgattcatta ccaagtgatc tctctgagtg acaactttat gttcatagtt
    26161 tgcttttgtt gcagcagata cagccaacag taataaaaca attgccgtta tattaactac
    26221 ttttctttgc ttagctgatg tgctcttcat gaaaacgaac gataaaatca acgagccaaa
    26281 cagtacttga aagcctaaga ttcgccaagg aaattgaatc atttgaatgg gtgtattttg
    26341 taataaagac catggaaaaa atttggtgga taagagccaa gagactaccg cactaaggta
    26401 aatcactttt tctttgcgat taaatccttt aaaaaagaag agtggacaga tcaaagaaat
    26461 aataccaatg attcctaccg taaaggttgt caggtcatta ttcatcgcac caatcaaact
    26521 atccgagaca tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat taatcgattg
    26581 aaccatgatt tgtcggaaca tcggatacca aaagtatgct gttaaaagaa tcgtcattcc
    26641 tactgccttt gacaactcca caatcaaccg gatgctttct ttttttcttg tttcacttac
    26701 tacaaagccg cctaagacta gtaagccaac ggctattaca ctcataaaag tggaaagaac
    26761 atgtgtataa atgagtaagc tcatactgac tgctaatgga attgcacatg gctttttctc
    26821 ataaatcagt gcgtaagcat aatacataac tatcggtata accgtaagtg caatcgcttc
    26881 agcaatagca gaacgatgat aaatatcaat cgtacggtac ccataaaatg tatataaaca
    26941 gctaaatata aaagccgcat cgattctttt taaaaatttt agtccataat gaaacatcag
    27001 catgatagta caaacattca atagccaaac atataagata taactaacga ttaaattatt
    27061 acttatccaa taaaaaatga ctgccggaaa aaaggtcaag tagggataaa aataattcac
    27121 tccgctccca taatgattaa acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa
    27181 ccctttgact ctagcaaaat ggaaggcaat atcccccgat gtagaaatag tatgaacatt
    27241 tctaaaataa ttgattgaat aaagtccagc aataattgct aggagaatgg acaatattat
    27301 ttttttcttt acttgcatta cttaattata tactcctttc atggattgct ttatctagta
    27361 taacttaaat tttaaataaa tcgttattta tttttactct cacttaaaaa agcactaaaa
    27421 ttataagaga gggaggtaat ggtctgaaca ccaagaattg ttccaacatc gaattgctta
    27481 actaatgagt tcaaagctta attatgtggc tcttatattt accgagcgtg gattgcgatc
    27541 tctttagcga aacaaacatc tttcataaaa aaaacagcca tatccgcttt cttggatatg
    27601 actatttact actactttac tatctttcat tcttcttgtc tcacttatga taaggcgccc
    27661 ccgcattgat ccggaaggca cggtagattt gctcgacaag gatcagacgc atcaattgat
    27721 gtggatacgt catttttcca aaagagatct gagcattgct tcttttcatg acggcttcgc
    27781 tgagtcctaa agagccgccg ataacaaaaa caaggtggct ttttccttgg atacctaatt
    27841 ggtcgatttg tttagcaaaa gattcacttg taggattttt cccttcaatt gctaaggcat
    27901 agacgtattc ttgttctttg attttcgcta agatccgttc cccttctttt tcttttactt
    27961 gtctcatttg cgcttcgctt aaattctccg gggctttttc gtcaggaact tcgatcaatt
    28021 cgatttttgc ataggcattc agccgtttga catactcatt gataccttgg atcaggtatt
    28081 tttcttttaa ttttcctact gagataattt tgatattcat gttttctctc ctttggtctt
    28141 catgctcact ttgtttatta taatcaattt tttcgtctat atgtttattt tggctatttt
    28201 tgttaaaaag atttccccaa cagttatgca caacatccac aggtttatcc acagaaaaca
    28261 gtgttatttt accttatttc atcacattca ttaggcaaac atacgtttta taagaattta
    28321 tccactttat acacaggctg tggatatgtt ttatccctta tttttccaat cctatccgcg
    28381 tagttttaca cagtatctgt ggaaaaagaa aatcaaatat tttcatctga aaacaatgat
    28441 ttttcctaaa gtttttcata aaaagttcac agtctcaaag tagactaaag tcaacaaata
    28501 agactatact aacagtagat aaatgaatgt aattggagga ggaacacatg gatagaaaaa
    28561 atgtgacacc aaagatgaaa aaaagtaaaa ataatagcct ctggcgtaag ttaggtctgg
    28621 ggcttgttgg agggatcgtt ggaggactac tgacagctgg cattttttat gcagcgatgg
    28681 gcactggcaa caatagttct actgctacaa gcggcaatca gaattctgcc ggtgaaacag
    28741 ttgtggaaaa tgtcaaagtc aatgtagatt ctgatatcac caaagcggta gataaagttc
    28801 aagacgctgt cgtttctgtt atcaacttac aaagtcaaaa ccaatcaagc ggttttggcg
    28861 gactattcgg gcagcaagaa gaaagtagca gctcagatga tagtaatttg gaagcttata
    28921 gtgaaggaag cggcgtgatc tacaaaaaag acgggaatac tgcttatgta gtcacgaata
    28981 atcacgtagt agatggccag caaggtcttg aagtcttgat gaaagacggt acgaaggtaa
    29041 aagctgaatt agttggaaca gatgcttatt cagatttagc ggtgttgaag atcaattcag
    29101 acaaagtcga aacagttgcc tcttttggtg actcaagtgc tctgaaggtt ggtgagcctg
    29161 cgattgcgat tggttcccca ctaggatctg aatatgcaaa ttctgtcact tcaggaatca
    29221 tttcttcctt gaaccgacaa gtgacaagta cgaatgaatc gaacgaaaca gtcaatatca
    29281 atgcgatcca aacagatgca gcaatcaacc ctggaaattc tggtggtcct ttagttaata
    29341 tcgaaggaca agttatcggt atcaactcaa gtaagatcgc aagtacttct gaatctactt
    29401 ctaatgtaag cgtagaagga atgggatttg ctattccaag taacgatgta gtgaatatca
    29461 tcaatcaact ggaaaaagac ggaaaagtga cacgtccagc tctaggtatc acaatggtcg
    29521 acttatctgc cgtttctact cagcaacaag aacaaatctt gaagattcct tcatctgtaa
    29581 ctaacggtgt catcgtgaca aatgtccaaa cagctacacc ggctgaaaaa gctggattga
    29641 aacaatacga cgtcattact aagatcgatg acaaagaagt ttcttctggt gtagaacttc
    29701 aatctgcttt gtataagaaa aaagtgggag attctgtcaa aatcacttac tatgaaggct
    29761 cgaaagaaaa aacaactact gttgaattga cgatcgatca atcggcatta aaacaaaatc
    29821 aaaatagtga aaattaataa aaaagaggtt gtgacaaaga cttgtcacaa cctctttatt
    29881 ttggaaatgc ttctccgact acggcatcct caggcaatac taagattcct ttttttgctg
    29941 gagcatttgg caaatgcaat tcacgagcgg aacagatcat tccatagcta tctacccctc
    30001 gcaaagttcc tggccagatc atcattccat ctggcatcat agctcctggt tttgcaacga
    30061 caactttttg tccagcttcg atattgggtg caccgcaaac gatttgcaat actgtaccat
    30121 tgtctacttc tgtctcagta atcgataaat ggtcagaatc aggatgtggg acgcatgttt
    30181 ttacgtaccc gacaacgatc ttcggttcat gatcagcttc aagctgttct tcgaatcctg
    30241 cttctttgat caatcgattc aatttcgcta attcctcatc tgttaagctt acttggccgt
    30301 tcccttcgat tgttagatga tcggagatat ggaaaaagtt ccaagcaaca acttgacctt
    30361 gttcatcttt gatccgagca acattccatt tgcgttcaac agtgttttct gttcctttat
    30421 cgtcggctat aataaccatc aaggtgtctc cgacgtgtgc tttgttataa gcaaaaatca
    30481 tgttctttcc tccattatcc tttgctgaga tttatcttaa caaaaaattg cattcactgc
    30541 atgaaatttt tttcatgctt tatttccgaa gaagcctctt ctggaacacc atttattcgt
    30601 aacaagggac tgtgacaaga cttttgtcac agtcccttta ctgttttagt taattgatta
    30661 aaaaggtcgt acaagttcca cttgcttaat tacttggctg ttcttcttcg tcagaaacga
    30721 tcaagacatc acaaggtgaa tgacgtatca cgtagctggc tacacttcct gtcataaagc
    30781 gttcgactgc atttagacct gactgcccaa ccatgattag atcgatctca tatttttttg
    30841 gcaatttgac tgccatggat tcacgaacag aaccatatgc aataattcct tctatatttg
    30901 tgtaatcaac agatttccca tattctttac attcttcgat catttccttg gctgtttcag
    30961 tttgctgatc gactagattt tgatcaatag gtgcatagcc cataaagtta taaaattgct
    31021 gatcgattac gtttgctaca tatactttcc cattattacg gcgagccact tctaccgctt
    31081 ttttgaaagc agcaagtgct tgttcactac cgtcgatacc tactaaaata ttcttatatt
    31141 cttgattcat cttgacccct cctaacagat gtcagccttt tgttacactt tctaggaacg
    31201 attcgatttc ctgttttgtt ttacgatctt tattcactag acgacctaat tcttttccgt
    31261 cttggatcac gacaaaactt gggataccaa aaatgttcca ttcagcggct acatctatgt
    31321 acttgtcacg gtctacttca ataaattgcc aatcagaaaa gtcattttca atttcaggca
    31381 tttgcggttt gataaaacgg cagtctccgc accagccggc agtaaagaaa aatacactct
    31441 ttccagtttc tacgtagcct gctaattctt ctaaactatt tgggataatc attctttgtc
    31501 ctctactttc tgtttatgta tttttcaatt aaattataca cctttctttc gaactaacaa
    31561 acattgatcc tctttttata aaaaaaatct ggcaaaaagc aatcttttgc cagattttat
    31621 agtttcacta tcgcattatt ttccgtatac gatcgtattt actgttgatt tatccaaacc
    31681 tttcagtgtt tgaatcaaca tttctcgtgc agcttcaaaa tcgcgaatac tgaacatcgt
    31741 ttgatgcgta tggatataac gtcccactac gccaatcact gtacttggga ttccttcgtt
    31801 ttgtgtatga gcagcacctg catctgtacc gccttttgaa acaaagtatt gataagggat
    31861 attgtttgtt tcagcgatat ccagcaagta ctcgcgcaaa cgaggaagca tgatcaaacc
    31921 tggatcttgg atacgcatca atgtaccttc tcctaaatgt ccgaatgtgc catttttcgt
    31981 cactgtatca tctgctgcag agcagtctac tgcaaagaac agatctggct tgaatttatt
    32041 gacagaagct ttagaaccac gcaagcctac ttcttcctgg acattcgcgc ctgcaatcaa
    32101 tgtatgacct aatgtttcat tttgcaacgc ttctaaagct tctaatacca ttgtacagcc
    32161 atagcggttg tcccatgatt tactgataat attctttccg ttggctgttt tgatcgtttc
    32221 agcgtaagga acgattgtgt ctcctggaag gacaccgtaa ctcattgcct cttctcttga
    32281 ttcaaaccca gcatcgaaca aaatatcggt tacttctact gatttttgtc cgcttgttcc
    32341 tcgcaataaa tgtgggggaa cggaagaaga aatacatgga taattcttac cagtagaggt
    32401 tttaagtgta aaacgctgag cagaaacaac gtaaggattc cagccgccta gaggtacgac
    32461 tttaaaaagc ccgttttctt ggatttgtgt cagcatgaat ccgacttcat ccatatgtgc
    32521 tgcgaccatt acacggggtg catccgcttc ttcatgatgg cgcagaccaa aaatgccgcc
    32581 caaaccatct aactgtaact catctaccag cggttccata tgactttcca tataagcgcg
    32641 aacatcttgt tcaaaaccgc ttgttccttg taattctgtt aactctttga ttcgttgaaa
    32701 tgttttttct tccatatgct attcgtctaa atttcagact aaactcctct cagacttata
    32761 ttgatctgcg gataaaatat gattttttct gcagaccgat cgtatttatt atagcttatt
    32821 ttttctggta gcggttattt atttcaaaga aaatatgaga gtgtggtaaa ataagcacaa
    32881 ggagtaacaa gtgagaagaa ggaggtatgt ccacatgtat gaagaaagcg aactaagcta
    32941 ttttaaaggc gggttagcag ttggaatcgg tctaggatta ttgagcgggg tagcttcaac
    33001 gatttggtat catcgcaaaa aagtattgtc agcagaccaa gtacttgaaa atatcaagtc
    33061 atcttttcta aaagaaggtg caatcgaagg ttcttggatc tcttttgaaa aggaacctat
    33121 taggaaattc gccgttcggt cgagtgctta tcggggaggt ctttctcgaa tagaagatgg
    33181 cgaaactgtt tattatgaat ttttagcaga tgctcataca ggaaccgtat tagaaatttc
    33241 aagaaaaaaa ggtgcaacag attaatacgt caaaaaagtg agagaacgaa tacgcaccca
    33301 ctattcaaaa gagaggtatc atgtccttct tttgtagtgg gtggtttttt agatagagct
    33361 ttccaaacat atattccagt attttctttt taaataaatg atatttagcg ggaaaaacta
    33421 gcatttcctt tgattatgag gtatttttaa tttagctttt gttttctata gttaaggaga
    33481 gtgaaaaatg gaacaacaag aaaaaattgc tgttctgatt ccttgttaca atgaggaagc
    33541 aacaatttca acagtcattg cagactttaa acgagaatta ccagaagcag atatttatgt
    33601 ctatgataat aattcgaccg atcgaacata cgaattggcc gttcaaggtg gagcaatcgt
    33661 aaaaaaagaa cctcgtcaag gaaaaggaaa tgtgatccgc caaatgttct ttgatatcga
    33721 tgccgactat tatttaatgg tagatggcga tgatacttat cctgcagaag ctgtacatgg
    33781 tttgctcgag aaattacgtt ctggtgaagc agatatggtc attggtgatc gcctatcaaa
    33841 tggaacctac tttgatgaga ataagagacc tttccacgat tttggaaata accttgttag
    33901 aaatacaatt actagaatgt ataaaacaaa aattcttgat gtcatgaccg gctatcgagg
    33961 attcaatcgt atctttgtca aaagttttcc tattatgagt tctggttttc aaatcgaaac
    34021 ggaattgacg atccatgctt tagataaaaa attcaagttc gttgaaatcc caatcgatta
    34081 tcgtgatcgt cctgaaggca gcgaatcaaa attgaataca ttctctgacg gattcaaagt
    34141 gatcatgatg attattaaaa tgtttaaaga ttataagcct ttgcttttct ttgggatttg
    34201 gaccattttc ttctttttat ttggattggt cacggggatt cctgtgattc gcgaatatat
    34261 gctgacaagc tttattacac gcatcccttc tgcgatttta agtactggat tgatgacttt
    34321 agcacttctt tcattagtaa cagggttgat tttggataca gtcgtcacga acgctaagaa
    34381 agaatatgaa ttgaatctat atcatgttta tcatgaatac cacagtagaa agtaaaaaaa
    34441 ggatcaagac tgcaaaaata atttgcaatt cttgatcctc tttttaatta attatttcgc
    34501 caaacgatag atggcatctg catagattgc tgttgctctg aacaagtcat caagactcat
    34561 aaattcgttt gcttgatgca ttgtatcagt atatcctggg aacatcgctc cataagcaac
    34621 gccacgtttc aacaaacgtc cgtaagttcc gccaccgatg atttgttcgt agcctttttc
    34681 tccagtgtgg tcttcataga cttgcaataa tgtagccacc aatggatcat ccattggtac
    34741 gtaatgtggt tccattacgc gagcacctcg agtaactgtt gcgccttctt gccccactgt
    34801 ttgttcgatg tcactttgca atccgtctac tgttacacct tttggaaaac ggaaattcat
    34861 attgatgaag ttcgcttcct ctgtaccatt ttcttcaaaa gcaaatagtc cagcattcat
    34921 tgtcaatttg cccatttttt catcttcata agcgactcct agtttttctc cataaaaatc
    34981 ttcatggaca taagcagctg ctacatggat aaattgttta gcagatccta ggaattcata
    35041 gttatctaag aacaatgcca agaaagaacc tgcattgata ccagattgtg ggcttgcacc
    35101 atgtgcgcct ttaccaacta attcgatttt tacatacgta ttgtccgctt caatcgttcc
    35161 gcttactgga tttgcttcga tgaattggta aaatgcttct tccatcgcga tagctgcatc
    35221 tgcacttggt acttgaagtg ctgcagttgc tgttcctggc accatatttt cgcgaagtcc
    35281 ggaaacaaaa gatttcaaga cataatctcc tgcattgtcg cctttgaagc gtaaagctaa
    35341 agaaacattt cctttttcac cattgatgat tgggaattca gcatctggtg agaaaccaaa
    35401 atctggttct tcttcatgtt cgaaataata agccatatcg ccccagccgc tttcttcatc
    35461 gctgccgact acgaagcgaa ctttttttga tagtttcaag tcaagttctt taatgatctt
    35521 catcgcatag taagctgcca tacttggacc tttgtcatcg cttgatccgc gtgcaaagat
    35581 ctttccatcc ttgattactg gttcataagg atctgtgtcc cagccatctc ctgcaggaac
    35641 gacatccatg tgtccaaata tccctagcgt ttcgtctcct tcgccaagat caatatgccc
    35701 tgcatagtta tcgacatttt ttactttaaa tccatcacgt tcgccataag ctaacatatg
    35761 tttcaatgcg tcgcgaggtc caggtccaaa tggtgcatct ggtgttactt ttgagtcatc
    35821 gcgctcgctg ttgacacgta aaagattttt caagtcttca agcaagtctt ctttacgtgc
    35881 ttctacttct ttttgccaat caattgtcat aaagttaatc ctccacttat ttaagtcttc
    35941 gtgttcatga taccacaaat tacagaaaaa aatagttcaa aaaatctgac aattcctttt
    36001 cttatctgtt tttctctaaa aaaaatatac gctataatga gtaaaaggag gtttttagat
    36061 gctgataaac gaacaactag tgaaaagagt catcacacct cgtccaaaaa tttcacataa
    36121 aggaacattt ggacgtatcg ttttgattgg cggcaatgaa caatatggcg gagcaatcat
    36181 tatgagtgct ttagctgccg tacacagcgg agcgggtctc acgactgtag taactgatga
    36241 aaaaaatcgg agtgctcttc acagtcatct gccagaagca atgattgtag tttggacgga
    36301 tactgaccag tttctttccg taataaagca agcagatgta ctcttgattg gtcccggatt
    36361 gggtacttct tctaagagtt tggaacgttt caaacaagta ctagaaaacc aatcaaaaga
    36421 acaatggctg gtgattgatg gctctgcttt aagtttactt gctgctaatc atcttttgct
    36481 accctatccc gaacaaactg tacttacacc tcatcaaatg gagtggcaac ggttgagttc
    36541 tctaccgata caagaacaac gggataaaga aaacaaaaag aagcaacagg aaatcggtgc
    36601 tgtgctcgtt ttgaaaagtc atgagacaaa aatttatggt caccagcact tctacaccaa
    36661 tcctttaggt tctgctgcta tggcaacggg cggcatgggt gacactttag ctgggatgat
    36721 cacaggattt ttagcccagt ttcctcaaaa tgaccaaacg attgcagcag ctatttatct
    36781 tcatagtttc attggagacc agctggcaaa agaacaatat attgttttgc cgacacaaat
    36841 cagcgaagca cttccgaaat ggatgaatta tttttgtaaa aaatcaatgg aaaacgaggc
    36901 atgagtcatg gagaaattta caggttatgt gcgagaacct ttttattatg aaacagatca
    36961 aatggggatt attcatcaca gcaattatat ccgctggttt gaagaggctc gggtagcctt
    37021 attggtacat ctagggtttc cctataaaaa aatcgaagaa tctgggatca tcatccccgt
    37081 tctggaagta aattgcagct ataaagaaat gatccattac ggagaaaaag tgagcatcca
    37141 tccaactatc caaaagtata cggggacacg gttggatttt agctacgaaa ttgtgggaac
    37201 tgaagacaaa aaattaaaaa ctactggcag tagtaaacac tgttttttac tttctgattc
    37261 gcaacgatta gtgaagctga gcaaagtcaa tccggagtta gatcagttat tccaagcgta
    37321 cgcttctttg gatgatgtaa attcttaata aagaccgtct aagcattttt cttgtattgt
    37381 gtcgagtagt tcgtataatg aaaagaaaaa agcaggtgat ccccatgtat gtcgttaaag
    37441 taatgcacgg ttacatcgat aaaactggct gtcgcactcg ggaaaaaaac ttagacaatc
    37501 tgctcatttt caaagacaaa aaagaatccg aagcttttgc taaaagaatc ggcggtcgtg
    37561 tcaaaccgat ccaagaggta cgaccagatt gattcaatat ttttcttttc gaggctctaa
    37621 taggcatgcc tatcagggct ttttttgcag actgtgtttt tatgcccaca cgatttgtaa
    37681 gcgatttatg ttataataga ttatatctgc atagaaagga aggttaaatg gttatcaata
    37741 atttgcaaca gcaagcttat gaatctatac gtaaacaaat catttattcc gagttagcac
    37801 ctggaagtaa aatctccgat aaagtgttag aagaacaact agctatcggg cgtacaccga
    37861 ttcgagaagc actgatccaa ctgcgcaatc aagagcttgt aaaaaccatt ccacagtccg
    37921 gcacttatgt ttcaaaaatc gatatccgat ctgcttcatt agcccgttat acgcgggaaa
    37981 agttggaaaa accgatcctg caagaatgca gtgcgaaaat gaccaagcaa gatcaagcta
    38041 aactagaaaa aatactagaa caaaccgatc aggcagtaat cgctcaaaat aaaaaagctt
    38101 tttttgattt ggatactgat tttcaccgaa cttgttatga aattgctgga aaacgagaaa
    38161 tttgggattg gctagaaagt tatagtacgc acttgaaccg tttcagatgg ttacgtttga
    38221 cgatttcaga attagactgg ggtcgagtat tagatgaaca tcagacaatg cttcaatcaa
    38281 tgattgatca taactttgat gaagtcggat ttctttgcac gatgcattta catatgatta
    38341 tcgaggagca agaatacgtg attcataatt accccgatta ttttgaagat attcaggaca
    38401 gacccatcaa ataagaggtt ttggaatcag cgttttgacc tgagtattag agaaagaaac
    38461 ggcaaaatag ttcctcatat tttgccgttt tttttgagct aataccgagg gtcagctgat
    38521 tgcacaccgt ttattgagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    38581 caagtaaaaa tagcttctta tgtttttaac ttggctggac ttattaccgc agatgcaagc
    38641 ttttgaacac cgttttccaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa
    38701 caatttcaaa aaacagcttc tcatgtttgt tgaatatttt gacttgtgac cgaggagttg
    38761 acaggagaac atcgtttatt cggaaaaaaa ggactgtgac acgacttttg tcacagtctc
    38821 tttatttgta atctaaatca tactccgtac atcttttttc tatttttttt atttcttcta
    38881 agatcccttg gctacgaatt tcattatatg gccaaagtaa gactgcctct aatacataag
    38941 gcgaaaaatc gctttgattc atctcttttg aaaaactcat cgctgctatc tcgctcaatg
    39001 cacgaatgtt tgattgatgt gtataaaaag gcaaccgcca cagcacaatt ttttcgcttt
    39061 gcgtataaat tcccttaaca gaagcttcga tgtggtgaaa tagttcatgt ccaataatca
    39121 aagcttgcgc tgtttcttta gagatcgaag ctagtcctga acattttaat ttttctaacg
    39181 gctccgtcat caatcgaatc ccttcatctt ctgcataagt tgcaaaagtg attcgttcgt
    39241 ttatttgctc tgattcacag gaaacgattg gaacagccat ttgttcacaa atttctccta
    39301 catcggcaaa agggtacttt tttttgatgc tacatgccgt ttcgatacca caatcgattg
    39361 attgttgtat aagaattttc tgcttggaca aaggaatctt tttccaaaat aaatctcgcc
    39421 gaaactggta gaatccccaa atttcttcgg ggatctgttg taattcgttt agtaactctt
    39481 ctaacttatg tttgtttgat atttccggca acgacgatca cttcttcctc cggttccata
    39541 gtagatactt ccagatccat caaaagctgt agttgttcca gatccgtcgc tgtaaaatca
    39601 gagactcttg ctcctaagca gagataaaat tctggtcggg caatcaattc agtttgataa
    39661 actgccatat cttcccaaag ttgttcgaca gctgtcatat aattggctgc agttgttttc
    39721 atacatgaag catgtccacg ttggaccttg ataaatcctt tttgatcgat gatccgaaga
    39781 cttccagcat tttttgaacg attctgttcg ccgcatacat aaaagaaagg tgtattttcc
    39841 aataagcaaa cttcattttt atttaatcgc atatcctctg cagcaagctc tagtgcttct
    39901 tctgttgtga tctccttggt tagatcggtt gctttcactt ccgtagaacc agtagcgata
    39961 gccgttactt ttgaagtctg tggatcgatt tctacgtgga cttcgatcga ttctggtgta
    40021 gcgcctgatt caatcgcttt attcatcgct tcatttttca acgatcgtat atcttcttta
    40081 cttggcgaag ggatgatacg ttctacaaca tctcggacca ttgcaagtgc tacaccaata
    40141 gaagaaatca cttcggcgtt ttcaggaatc gaatatttca cacccatttt attactaaag
    40201 taagtaatca atgaggcagc gccgccccct acacctacaa gagaaatctg atctttctcc
    40261 aagtgatact tgtctgccaa ttctaaaatc accggttcaa tcttagcata ggatttttcc
    40321 atgatctgtt ctgcgatgtc ttcaaccgtc gtatgacaat aatcagctaa tgcttggatg
    40381 gcttttcgtg cagatgggac atttccataa gaaaaatgtt cttcttgcac taatccaagg
    40441 acattggctg cacatgtatt tgtaatagtc acttcttctc cgtcttccat cactaccttg
    40501 acgtaatcag cagggtctcc ttctttagga gagaaaaatt caaccttagg acctttgatt
    40561 ttttctgtct ccgtaaatac tgcataatct agaccggcaa tatgtgcaga acgtggaccg
    40621 acatcgatga ttcctgactg attagcccgt accatcgaac cgcctgccac acctagaacc
    40681 cgaacatcta atgaagagat atatgttggg tggccaccta caattgaata atcgattgct
    40741 ggacgtccat ttttaatgac cccgatattg gttgttgttc cgcctacttc aaaataaaca
    40801 ccattagacg ctctcaagta catcaaagag cccatgactg atgctgctgg accggaaagc
    40861 atggttaaaa caggtcgttt tttcatttca ttgatttcca ttactccgcc gtctccgcgc
    40921 ataatcatca aagaaacatt gacccctgct tctcgtacgg aatcttctgt agaggttgcc
    40981 gtatctagca tttttggtaa aatagatgca ttgattgctg ctgtccgagt tcttcttgtc
    41041 aatccatata attttgtgat gtcagaagcc atcgttgtag ggatcgattt taccgatgct
    41101 gcttcataaa caactcgttc tggttcccca ttatctactc caaatgccat agaagaaacc
    41161 aagacctccg ctctttctct ttctaaagaa gaaatcgttt ctgaaacacg gtctacattt
    41221 aagtgtttga caggtaagaa tgcattgaca atctctattt ttttcttatt ccctaaatcg
    41281 atatcgttca atctagtttg tcgttttgct aaaaaacctt ctaatccacc ttttgccatc
    41341 ccgattacgc cgacttttgc tacgtctcct tcaatcagtg cattagtcgc ttgtgtggta
    41401 gaatgtgcaa caaacacaac atcttctgga ctgatattat tttccagcaa acaattttga
    41461 aaagactgaa cgacacctgc ggctactcca cggacatcat cgtgggttgt tttcactgaa
    41521 gattttccga tgatttcgtg cgtcgcatta tcgattgcga ctgctttcgt atgtgtaccg
    41581 ccaacatcaa ttcccattcg cacaaaacgt ttcattttct tttcctccat tcatctaatc
    41641 gtttttatcg acgttttgtt tactaggcgt tcccgtacaa taagaacgtg atcaggcata
    41701 agatcgctcc gatgatccaa ccagttggga tggatttctt catatattct cgagaagtta
    41761 cttttgtata acctaatccc caagcaaccc aagattgtgt aatatctaaa tgctgaggag
    41821 caatcgttgt gattgcaaat aacgggaaca aaaaggctgg cgacatcgtt ggattaacag
    41881 ctaatacgac agctaaaata gctgagcctg aaccgactag attcattgga ccgcggaaaa
    41941 atcctagtgg tgtcaatata gcaaatacga ttgccaagac taatggtgat tgcggtatcc
    42001 aatctccaaa aatagctgaa aaataaggag aagcatacgc agctgaatta ttgaacatcg
    42061 ctaaggtcat caagaaacca accattggcg caacatctac tgcgccatct gtaaataatt
    42121 tcgcaaactg acgacagatt tctttataac ctccgcgcaa ttttccagta aacagcaatg
    42181 cccagatacc tgctagaata aaacctaaga tgattggaag atctaaaaga acgaccccta
    42241 aaactggtaa taatacggct aaccatgaga tcattggtgc gtcgccacct tcagctggta
    42301 cgtttgctgc cattgcatag cgtttctttt tgttcattga tatattagcg accgttagta
    42361 ccacaactaa gcttacaatc atcccaatca taccaatttg gaaatagtca ttataagtat
    42421 agctttcagc cgctggattg aaaccagcat aaatcgtttg atattgtttg aaattgacga
    42481 tattagcaaa gattccagcc ataatcgagc ccataaatga gaacagtgca accggtactg
    42541 aaatacctaa tgacatcaag attggcaaca caataactgc aatagaaatc actggtccga
    42601 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
    42661 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
    42721 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
    42781 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
    42841 tcgccgggtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
    42901 tgatggcaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
    42961 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
    43021 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
    43081 ctttcattaa agggtgacca taaatgttca gttttcaatt aaaaaacctt aattatgcta
    43141 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
    43201 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
    43261 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
    43321 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
    43381 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
    43441 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
    43501 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
    43561 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
    43621 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
    43681 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
    43741 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
    43801 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
    43861 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
    43921 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
    43981 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
    44041 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
    44101 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
    44161 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
    44221 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
    44281 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
    44341 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
    44401 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
    44461 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
    44521 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
    44581 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
    44641 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
    44701 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
    44761 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
    44821 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
    44881 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
    44941 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
    45001 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
    45061 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
    45121 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
    45181 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
    45241 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
    45301 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
    45361 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
    45421 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
    45481 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
    45541 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
    45601 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
    45661 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
    45721 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
    45781 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
    45841 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
    45901 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
    45961 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
    46021 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
    46081 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
    46141 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
    46201 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
    46261 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
    46321 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
    46381 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
    46441 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
    46501 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
    46561 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
    46621 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
    46681 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
    46741 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
    46801 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
    46861 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
    46921 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
    46981 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
    47041 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
    47101 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
    47161 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
    47221 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
    47281 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
    47341 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
    47401 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
    47461 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
    47521 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
    47581 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
    47641 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
    47701 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
    47761 tattagaaaa tgtccagctt tcggaaaaaa tcgcccatgg taatcctagt ggaatcgatg
    47821 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
    47881 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
    47941 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
    48001 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
    48061 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
    48121 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
    48181 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
    48241 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
    48301 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
    48361 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
    48421 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
    48481 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
    48541 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
    48601 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
    48661 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
    48721 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
    48781 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
    48841 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
    48901 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
    48961 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
    49021 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
    49081 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
    49141 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
    49201 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
    49261 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
    49321 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
    49381 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
    49441 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
    49501 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
    49561 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
    49621 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
    49681 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
    49741 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
    49801 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
    49861 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
    49921 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
    49981 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
    50041 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
    50101 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
    50161 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
    50221 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
    50281 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
    50341 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
    50401 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
    50461 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
    50521 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
    50581 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
    50641 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
    50701 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
    50761 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
    50821 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
    50881 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
    50941 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
    51001 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
    51061 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
    51121 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
    51181 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
    51241 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
    51301 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
    51361 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
    51421 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
    51481 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
    51541 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
    51601 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
    51661 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
    51721 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
    51781 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
    51841 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
    51901 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
    51961 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
    52021 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
    52081 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
    52141 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
    52201 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
    52261 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
    52321 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
    52381 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
    52441 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
    52501 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
    52561 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
    52621 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
    52681 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
    52741 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
    52801 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
    52861 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
    52921 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
    52981 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
    53041 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
    53101 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    53161 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    53221 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    53281 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    53341 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    53401 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    53461 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    53521 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    53581 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    53641 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    53701 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    53761 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    53821 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    53881 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    53941 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    54001 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    54061 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    54121 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    54181 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    54241 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    54301 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    54361 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    54421 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    54481 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    54541 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    54601 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    54661 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    54721 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    54781 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    54841 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    54901 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    54961 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    55021 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    55081 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    55141 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    55201 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    55261 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    55321 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    55381 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    55441 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    55501 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    55561 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    55621 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    55681 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    55741 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    55801 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    55861 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    55921 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    55981 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    56041 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    56101 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    56161 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    56221 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    56281 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    56341 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    56401 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    56461 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    56521 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    56581 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    56641 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    56701 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    56761 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    56821 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    56881 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    56941 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    57001 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    57061 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    57121 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    57181 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    57241 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    57301 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    57361 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    57421 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    57481 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    57541 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    57601 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    57661 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    57721 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    57781 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    57841 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    57901 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    57961 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    58021 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    58081 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    58141 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    58201 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    58261 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    58321 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    58381 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    58441 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    58501 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    58561 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    58621 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    58681 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    58741 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    58801 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    58861 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    58921 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    58981 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    59041 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    59101 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    59161 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    59221 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    59281 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    59341 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    59401 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    59461 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    59521 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    59581 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    59641 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    59701 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    59761 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    59821 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    59881 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    59941 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    60001 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    60061 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    60121 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    60181 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    60241 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    60301 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    60361 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    60421 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    60481 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    60541 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    60601 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    60661 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    60721 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    60781 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    60841 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    60901 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    60961 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    61021 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    61081 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    61141 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    61201 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    61261 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    61321 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    61381 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    61441 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    61501 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    61561 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    61621 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    61681 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    61741 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    61801 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    61861 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    61921 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    61981 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    62041 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    62101 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    62161 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    62221 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    62281 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    62341 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    62401 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    62461 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    62521 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    62581 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    62641 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    62701 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    62761 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    62821 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    62881 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    62941 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    63001 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    63061 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    63121 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    63181 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    63241 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    63301 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    63361 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    63421 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    63481 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    63541 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    63601 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    63661 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    63721 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    63781 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    63841 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    63901 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    63961 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    64021 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    64081 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    64141 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    64201 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    64261 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    64321 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    64381 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    64441 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    64501 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    64561 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    64621 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    64681 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    64741 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    64801 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    64861 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    64921 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    64981 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    65041 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    65101 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    65161 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    65221 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    65281 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    65341 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    65401 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    65461 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    65521 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    65581 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    65641 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    65701 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    65761 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    65821 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    65881 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    65941 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    66001 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    66061 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    66121 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    66181 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    66241 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    66301 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    66361 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    66421 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    66481 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    66541 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    66601 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    66661 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    66721 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    66781 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    66841 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    66901 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    66961 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    67021 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    67081 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    67141 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    67201 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    67261 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    67321 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    67381 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    67441 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    67501 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    67561 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    67621 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    67681 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    67741 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    67801 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    67861 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    67921 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    67981 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    68041 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    68101 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    68161 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    68221 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    68281 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    68341 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    68401 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    68461 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    68521 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    68581 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    68641 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    68701 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    68761 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    68821 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    68881 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    68941 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    69001 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    69061 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    69121 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    69181 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    69241 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    69301 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    69361 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    69421 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    69481 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    69541 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    69601 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    69661 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    69721 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    69781 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    69841 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    69901 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    69961 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    70021 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    70081 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    70141 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    70201 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    70261 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    70321 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    70381 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    70441 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    70501 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    70561 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    70621 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    70681 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    70741 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    70801 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    70861 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    70921 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    70981 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    71041 aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag
    71101 gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta
    71161 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
    71221 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
    71281 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
    71341 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
    71401 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
    71461 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
    71521 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
    71581 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
    71641 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
    71701 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
    71761 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
    71821 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
    71881 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
    71941 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
    72001 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
    72061 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
    72121 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
    72181 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
    72241 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
    72301 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
    72361 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
    72421 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
    72481 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
    72541 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
    72601 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
    72661 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
    72721 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
    72781 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
    72841 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
    72901 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
    72961 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
    73021 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
    73081 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
    73141 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
    73201 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
    73261 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
    73321 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
    73381 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
    73441 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
    73501 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
    73561 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
    73621 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
    73681 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
    73741 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
    73801 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
    73861 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
    73921 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
    73981 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
    74041 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
    74101 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
    74161 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
    74221 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
    74281 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
    74341 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
    74401 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
    74461 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
    74521 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
    74581 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
    74641 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
    74701 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
    74761 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
    74821 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
    74881 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
    74941 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
    75001 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
    75061 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
    75121 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
    75181 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
    75241 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
    75301 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
    75361 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
    75421 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
    75481 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
    75541 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
    75601 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
    75661 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
    75721 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
    75781 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
    75841 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
    75901 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
    75961 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
    76021 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
    76081 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
    76141 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
    76201 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
    76261 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
    76321 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
    76381 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
    76441 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
    76501 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
    76561 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
    76621 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
    76681 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
    76741 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
    76801 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
    76861 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
    76921 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
    76981 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
    77041 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
    77101 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
    77161 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
    77221 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
    77281 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
    77341 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
    77401 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
    77461 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
    77521 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
    77581 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
    77641 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
    77701 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
    77761 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
    77821 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
    77881 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
    77941 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
    78001 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
    78061 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
    78121 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
    78181 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
    78241 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
    78301 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
    78361 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
    78421 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
    78481 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
    78541 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
    78601 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
    78661 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
    78721 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
    78781 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
    78841 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
    78901 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
    78961 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
    79021 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
    79081 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
    79141 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
    79201 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
    79261 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
    79321 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
    79381 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
    79441 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
    79501 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
    79561 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
    79621 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
    79681 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
    79741 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
    79801 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
    79861 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
    79921 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
    79981 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
    80041 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
    80101 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
    80161 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
    80221 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
    80281 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
    80341 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
    80401 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
    80461 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
    80521 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
    80581 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
    80641 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
    80701 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
    80761 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
    80821 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
    80881 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
    80941 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
    81001 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
    81061 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    81121 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    81181 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    81241 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    81301 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    81361 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    81421 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    81481 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    81541 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    81601 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    81661 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    81721 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    81781 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    81841 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    81901 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    81961 cttatgggca aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    82021 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    82081 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    82141 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    82201 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    82261 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    82321 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    82381 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    82441 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    82501 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    82561 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    82621 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    82681 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    82741 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    82801 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    82861 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    82921 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    82981 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    83041 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    83101 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    83161 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    83221 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    83281 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    83341 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    83401 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    83461 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    83521 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    83581 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    83641 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    83701 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    83761 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    83821 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    83881 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    83941 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    84001 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    84061 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    84121 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    84181 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    84241 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    84301 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    84361 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    84421 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    84481 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    84541 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    84601 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    84661 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    84721 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    84781 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    84841 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    84901 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    84961 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    85021 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    85081 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    85141 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    85201 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    85261 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    85321 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    85381 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    85441 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    85501 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    85561 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    85621 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    85681 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    85741 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    85801 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    85861 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    85921 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    85981 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    86041 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    86101 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    86161 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    86221 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    86281 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    86341 aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg
    86401 ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa
    86461 aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga
    86521 agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg
    86581 tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa
    86641 attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct
    86701 tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa
    86761 aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac
    86821 tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat
    86881 ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca
    86941 aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca
    87001 gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag
    87061 aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag
    87121 atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc
    87181 atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca
    87241 tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt
    87301 gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct
    87361 ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac
    87421 agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc
    87481 gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt
    87541 ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct
    87601 gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca
    87661 gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg
    87721 atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt
    87781 tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg
    87841 atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca
    87901 ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt
    87961 gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc
    88021 ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta
    88081 ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat
    88141 tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt
    88201 tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc
    88261 gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt
    88321 tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca
    88381 tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc
    88441 gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt
    88501 atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga
    88561 ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct
    88621 tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg
    88681 atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg
    88741 atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca
    88801 cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc
    88861 ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg
    88921 tccaaatttt catctgttga taaaatcata tctttaactt tattgatact tactaggttc
    88981 gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg
    89041 ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt
    89101 gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca
    89161 ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac
    89221 tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat
    89281 tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga
    89341 aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat
    89401 ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg
    89461 atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat
    89521 cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta
    89581 ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg
    89641 acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta
    89701 cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct
    89761 attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt
    89821 cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc
    89881 cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag
    89941 acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag
    90001 caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct
    90061 ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt
    90121 tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt
    90181 ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag
    90241 cttctaattt ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc
    90301 ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca
    90361 ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc
    90421 ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt
    90481 ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc
    90541 gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat
    90601 cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca
    90661 aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac
    90721 cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt
    90781 taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct
    90841 ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt
    90901 cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct
    90961 aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa
    91021 aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa
    91081 agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg
    91141 tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa
    91201 attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc
    91261 caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa
    91321 agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta
    91381 cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta
    91441 gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc
    91501 ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg
    91561 atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca
    91621 atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc
    91681 gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc
    91741 tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga
    91801 tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata
    91861 gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt
    91921 gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg
    91981 atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc
    92041 ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc
    92101 ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac
    92161 ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc
    92221 gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt
    92281 ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg
    92341 aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc
    92401 ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt
    92461 gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc
    92521 agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc
    92581 agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc
    92641 tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc
    92701 ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc
    92761 aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc
    92821 aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc
    92881 ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc
    92941 tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac
    93001 ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa
    93061 aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc
    93121 ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg
    93181 gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat
    93241 cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt
    93301 ttctcttttc aataaattta gaacactctc attctaccaa attataacca ttttgccaca
    93361 aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta
    93421 tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc
    93481 gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata
    93541 attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta
    93601 tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc
    93661 tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc
    93721 taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc
    93781 gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg
    93841 taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt
    93901 tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg
    93961 gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga
    94021 aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt
    94081 aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc
    94141 acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc
    94201 gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt
    94261 ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc
    94321 tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg
    94381 cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt
    94441 ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt
    94501 ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt
    94561 acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc
    94621 gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg
    94681 tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc
    94741 tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg
    94801 tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg
    94861 cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag
    94921 ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg
    94981 ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg
    95041 tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca
    95101 ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca
    95161 agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct
    95221 aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc
    95281 taccacttca aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc
    95341 aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat agaacacagg
    95401 tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag
    95461 gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa
    95521 agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg
    95581 cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc
    95641 gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga
    95701 aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt
    95761 atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc
    95821 tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc
    95881 gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag
    95941 caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc
    96001 cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat
    96061 cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc
    96121 gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt
    96181 gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc
    96241 agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc
    96301 aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa
    96361 aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca
    96421 aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt
    96481 accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt
    96541 ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt
    96601 tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg
    96661 ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga
    96721 acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa
    96781 aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca
    96841 ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct
    96901 ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac
    96961 cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt
    97021 tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat
    97081 tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga
    97141 acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg
    97201 attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt
    97261 tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa
    97321 agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta
    97381 agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg
    97441 attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat
    97501 gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa
    97561 acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc
    97621 tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct
    97681 gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg
    97741 taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc
    97801 tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta
    97861 aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat
    97921 ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga
    97981 ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc
    98041 aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag
    98101 tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag
    98161 gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg
    98221 ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg
    98281 tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt
    98341 caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg
    98401 gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc
    98461 ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg
    98521 atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa
    98581 aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca
    98641 gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg
    98701 gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa
    98761 ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac
    98821 aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg
    98881 tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag
    98941 agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata
    99001 ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac
    99061 tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc
    99121 ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa
    99181 gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca
    99241 cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt
    99301 tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc
    99361 tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag
    99421 atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc
    99481 ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg
    99541 aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt
    99601 tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg
    99661 tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg
    99721 tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga
    99781 aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt
    99841 ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga
    99901 gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac
    99961 atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa
   100021 taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac
   100081 gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg
   100141 ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct
   100201 tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa
   100261 gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta
   100321 taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct
   100381 ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct
   100441 aattttatga catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt
   100501 tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt
   100561 gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag
   100621 cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt
   100681 catcaataaa aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat
   100741 ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc
   100801 tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa
   100861 gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa
   100921 acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa
   100981 atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa
   101041 taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg
   101101 caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa
   101161 gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa
   101221 aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt
   101281 tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc
   101341 catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa
   101401 ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc
   101461 catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag
   101521 ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac
   101581 gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt
   101641 gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc
   101701 ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa
   101761 taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa
   101821 attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata
   101881 actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat
   101941 tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg
   102001 attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc
   102061 aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa
   102121 accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc
   102181 gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag
   102241 ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg
   102301 attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg
   102361 aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa
   102421 ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt
   102481 taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata
   102541 agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa cttccggcca
   102601 tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc
   102661 catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag
   102721 catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa tctgcataaa
   102781 ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa
   102841 gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa
   102901 ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata
   102961 gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa
   103021 aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa
   103081 aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc
   103141 aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc
   103201 ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt
   103261 ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc atttattaaa
   103321 actgcctcct t
//
LOCUS       DACEDO010000002        98004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEDO010000002 DACEDO010000000
VERSION     DACEDO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 98004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.002"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(178..2070)
                     /locus_tag="IWO52_000098"
     CDS             complement(178..2070)
                     /locus_tag="IWO52_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354398.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2067..3794)
                     /locus_tag="IWO52_000099"
     CDS             complement(2067..3794)
                     /locus_tag="IWO52_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354399.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3810..4460)
                     /locus_tag="IWO52_000100"
     CDS             complement(3810..4460)
                     /locus_tag="IWO52_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0354400.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4664..4960
                     /locus_tag="IWO52_000101"
     CDS             4664..4960
                     /locus_tag="IWO52_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0354401.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5132..5752
                     /locus_tag="IWO52_000102"
     CDS             5132..5752
                     /locus_tag="IWO52_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0354402.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5844..7010)
                     /locus_tag="IWO52_000103"
     CDS             complement(5844..7010)
                     /locus_tag="IWO52_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0354403.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7022..7375)
                     /locus_tag="IWO52_000104"
     CDS             complement(7022..7375)
                     /locus_tag="IWO52_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0354404.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7503..8525
                     /locus_tag="IWO52_000105"
     CDS             7503..8525
                     /locus_tag="IWO52_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0354405.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8964..9398)
                     /locus_tag="IWO52_000106"
     CDS             complement(8964..9398)
                     /locus_tag="IWO52_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354406.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9508..11367)
                     /locus_tag="IWO52_000107"
     CDS             complement(9508..11367)
                     /locus_tag="IWO52_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0354407.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11485..12645)
                     /locus_tag="IWO52_000108"
     CDS             complement(11485..12645)
                     /locus_tag="IWO52_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0354408.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12952..14376)
                     /gene="araA"
                     /locus_tag="IWO52_000109"
     CDS             complement(12952..14376)
                     /gene="araA"
                     /locus_tag="IWO52_000109"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0354409.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14392..15090)
                     /locus_tag="IWO52_000110"
     CDS             complement(14392..15090)
                     /locus_tag="IWO52_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0354410.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15047..16708)
                     /locus_tag="IWO52_000111"
     CDS             complement(15047..16708)
                     /locus_tag="IWO52_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0354411.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16926..18326
                     /locus_tag="IWO52_000112"
     CDS             16926..18326
                     /locus_tag="IWO52_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0354412.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18578..19657)
                     /locus_tag="IWO52_000113"
     CDS             complement(18578..19657)
                     /locus_tag="IWO52_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0354413.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19743..20702)
                     /locus_tag="IWO52_000114"
     CDS             complement(19743..20702)
                     /locus_tag="IWO52_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0354414.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20714..22237)
                     /locus_tag="IWO52_000115"
     CDS             complement(20714..22237)
                     /locus_tag="IWO52_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0354415.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22247..22810)
                     /locus_tag="IWO52_000116"
     CDS             complement(22247..22810)
                     /locus_tag="IWO52_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0354416.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22816..23679)
                     /locus_tag="IWO52_000117"
     CDS             complement(22816..23679)
                     /locus_tag="IWO52_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0354417.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23693..24589)
                     /locus_tag="IWO52_000118"
     CDS             complement(23693..24589)
                     /locus_tag="IWO52_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0354418.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24764..26035)
                     /locus_tag="IWO52_000119"
     CDS             complement(24764..26035)
                     /locus_tag="IWO52_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0354419.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26025..27542)
                     /locus_tag="IWO52_000120"
     CDS             complement(26025..27542)
                     /locus_tag="IWO52_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0354420.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27740..28630
                     /locus_tag="IWO52_000121"
     CDS             27740..28630
                     /locus_tag="IWO52_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0354421.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="IWO52_000122"
     CDS             complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="IWO52_000122"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0354422.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29539..31011)
                     /locus_tag="IWO52_000123"
     CDS             complement(29539..31011)
                     /locus_tag="IWO52_000123"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0354423.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31648..32010)
                     /locus_tag="IWO52_000124"
     CDS             complement(31648..32010)
                     /locus_tag="IWO52_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0354424.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32233..32985
                     /locus_tag="IWO52_000125"
     CDS             32233..32985
                     /locus_tag="IWO52_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0354425.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33125..34048)
                     /locus_tag="IWO52_000126"
     CDS             complement(33125..34048)
                     /locus_tag="IWO52_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0354426.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34063..34335)
                     /locus_tag="IWO52_000127"
     CDS             complement(34063..34335)
                     /locus_tag="IWO52_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354427.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(34805..36430)
                     /gene="groL"
                     /locus_tag="IWO52_000128"
     CDS             complement(34805..36430)
                     /gene="groL"
                     /locus_tag="IWO52_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0354428.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(36481..36765)
                     /locus_tag="IWO52_000129"
     CDS             complement(36481..36765)
                     /locus_tag="IWO52_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0354429.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            36990..37652
                     /locus_tag="IWO52_000130"
     CDS             36990..37652
                     /locus_tag="IWO52_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0354430.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(37728..39020)
                     /locus_tag="IWO52_000131"
     CDS             complement(37728..39020)
                     /locus_tag="IWO52_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0354431.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            39190..39819
                     /locus_tag="IWO52_000132"
     CDS             39190..39819
                     /locus_tag="IWO52_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ0354432.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(39922..40731)
                     /locus_tag="IWO52_000133"
     CDS             complement(39922..40731)
                     /locus_tag="IWO52_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0354433.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(40786..41655)
                     /locus_tag="IWO52_000134"
     CDS             complement(40786..41655)
                     /locus_tag="IWO52_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354434.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(41656..42972)
                     /locus_tag="IWO52_000135"
     CDS             complement(41656..42972)
                     /locus_tag="IWO52_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354435.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(42969..44804)
                     /gene="walK"
                     /locus_tag="IWO52_000136"
     CDS             complement(42969..44804)
                     /gene="walK"
                     /locus_tag="IWO52_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0354436.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(44809..45513)
                     /locus_tag="IWO52_000137"
     CDS             complement(44809..45513)
                     /locus_tag="IWO52_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0354437.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(45703..46563)
                     /locus_tag="IWO52_000138"
     CDS             complement(45703..46563)
                     /locus_tag="IWO52_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0354438.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(46550..47119)
                     /locus_tag="IWO52_000139"
     CDS             complement(46550..47119)
                     /locus_tag="IWO52_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0354439.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            47500..47572
                     /locus_tag="IWO52_000140"
     tRNA            47500..47572
                     /locus_tag="IWO52_000140"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47533..47535,aa:Lys,seq:ctt)
     gene            complement(48068..48454)
                     /locus_tag="IWO52_000141"
     CDS             complement(48068..48454)
                     /locus_tag="IWO52_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0354440.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(48451..48972)
                     /locus_tag="IWO52_000142"
     CDS             complement(48451..48972)
                     /locus_tag="IWO52_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0354441.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(48974..50008)
                     /gene="sppA"
                     /locus_tag="IWO52_000143"
     CDS             complement(48974..50008)
                     /gene="sppA"
                     /locus_tag="IWO52_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0354442.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(50041..51456)
                     /locus_tag="IWO52_000144"
     CDS             complement(50041..51456)
                     /locus_tag="IWO52_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ0354443.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(51425..53578)
                     /locus_tag="IWO52_000145"
     CDS             complement(51425..53578)
                     /locus_tag="IWO52_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ0354444.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            53836..54072
                     /locus_tag="IWO52_000146"
     CDS             53836..54072
                     /locus_tag="IWO52_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0354445.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            54091..54306
                     /locus_tag="IWO52_000147"
     CDS             54091..54306
                     /locus_tag="IWO52_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0354446.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(55009..55761)
                     /locus_tag="IWO52_000148"
     CDS             complement(55009..55761)
                     /locus_tag="IWO52_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0354447.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(55776..57023)
                     /locus_tag="IWO52_000149"
     CDS             complement(55776..57023)
                     /locus_tag="IWO52_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0354448.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(57099..57245)
                     /locus_tag="IWO52_000150"
     CDS             complement(57099..57245)
                     /locus_tag="IWO52_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0354449.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(57349..57660)
                     /locus_tag="IWO52_000151"
     CDS             complement(57349..57660)
                     /locus_tag="IWO52_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0354450.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(57662..57859)
                     /locus_tag="IWO52_000152"
     CDS             complement(57662..57859)
                     /locus_tag="IWO52_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0354451.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(58253..59980)
                     /locus_tag="IWO52_000153"
     CDS             complement(58253..59980)
                     /locus_tag="IWO52_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0354452.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(59973..61166)
                     /locus_tag="IWO52_000154"
     CDS             complement(59973..61166)
                     /locus_tag="IWO52_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0354453.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(61353..61997)
                     /locus_tag="IWO52_000155"
     CDS             complement(61353..61997)
                     /locus_tag="IWO52_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0354454.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(62091..62225)
                     /locus_tag="IWO52_000156"
     CDS             complement(62091..62225)
                     /locus_tag="IWO52_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0354455.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(62227..64359)
                     /gene="feoB"
                     /locus_tag="IWO52_000157"
     CDS             complement(62227..64359)
                     /gene="feoB"
                     /locus_tag="IWO52_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0354456.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(64356..64829)
                     /locus_tag="IWO52_000158"
     CDS             complement(64356..64829)
                     /locus_tag="IWO52_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0354457.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            65230..65454
                     /gene="nrdH"
                     /locus_tag="IWO52_000159"
     CDS             65230..65454
                     /gene="nrdH"
                     /locus_tag="IWO52_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0354458.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            65613..67772
                     /gene="nrdE"
                     /locus_tag="IWO52_000160"
     CDS             65613..67772
                     /gene="nrdE"
                     /locus_tag="IWO52_000160"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0354459.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            67822..68787
                     /gene="nrdF"
                     /locus_tag="IWO52_000161"
     CDS             67822..68787
                     /gene="nrdF"
                     /locus_tag="IWO52_000161"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0354460.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(68876..70015)
                     /locus_tag="IWO52_000162"
     CDS             complement(68876..70015)
                     /locus_tag="IWO52_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0354461.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(70324..71037)
                     /locus_tag="IWO52_000163"
     CDS             complement(70324..71037)
                     /locus_tag="IWO52_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0354462.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(71291..71992)
                     /gene="nagB"
                     /locus_tag="IWO52_000164"
     CDS             complement(71291..71992)
                     /gene="nagB"
                     /locus_tag="IWO52_000164"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0354463.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            72226..73758
                     /locus_tag="IWO52_000165"
     CDS             72226..73758
                     /locus_tag="IWO52_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0354464.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(73949..75151)
                     /locus_tag="IWO52_000166"
     CDS             complement(73949..75151)
                     /locus_tag="IWO52_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0354465.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(75233..75922)
                     /locus_tag="IWO52_000167"
     CDS             complement(75233..75922)
                     /locus_tag="IWO52_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0354466.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(76028..76396)
                     /gene="rplL"
                     /locus_tag="IWO52_000168"
     CDS             complement(76028..76396)
                     /gene="rplL"
                     /locus_tag="IWO52_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0354467.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(76449..76952)
                     /locus_tag="IWO52_000169"
     CDS             complement(76449..76952)
                     /locus_tag="IWO52_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0354468.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(77362..78051)
                     /gene="rplA"
                     /locus_tag="IWO52_000170"
     CDS             complement(77362..78051)
                     /gene="rplA"
                     /locus_tag="IWO52_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0354469.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(78155..78577)
                     /gene="rplK"
                     /locus_tag="IWO52_000171"
     CDS             complement(78155..78577)
                     /gene="rplK"
                     /locus_tag="IWO52_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0354470.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            78920..79588
                     /gene="sdaAB"
                     /locus_tag="IWO52_000172"
     CDS             78920..79588
                     /gene="sdaAB"
                     /locus_tag="IWO52_000172"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0354471.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            79601..80473
                     /gene="sdaAA"
                     /locus_tag="IWO52_000173"
     CDS             79601..80473
                     /gene="sdaAA"
                     /locus_tag="IWO52_000173"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0354472.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(80564..81583)
                     /locus_tag="IWO52_000174"
     CDS             complement(80564..81583)
                     /locus_tag="IWO52_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0354473.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(81756..82643)
                     /locus_tag="IWO52_000175"
     CDS             complement(81756..82643)
                     /locus_tag="IWO52_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0354474.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(82720..83265)
                     /gene="nusG"
                     /locus_tag="IWO52_000176"
     CDS             complement(82720..83265)
                     /gene="nusG"
                     /locus_tag="IWO52_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0354475.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(83366..83536)
                     /gene="secE"
                     /locus_tag="IWO52_000177"
     CDS             complement(83366..83536)
                     /gene="secE"
                     /locus_tag="IWO52_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0354476.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(83560..83712)
                     /gene="rpmG"
                     /locus_tag="IWO52_000178"
     CDS             complement(83560..83712)
                     /gene="rpmG"
                     /locus_tag="IWO52_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0354477.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            83874..84518
                     /locus_tag="IWO52_000179"
     CDS             83874..84518
                     /locus_tag="IWO52_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0354478.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(84574..85077)
                     /locus_tag="IWO52_000180"
     CDS             complement(84574..85077)
                     /locus_tag="IWO52_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0354479.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(85090..85136)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            85674..86639
                     /locus_tag="IWO52_000181"
     CDS             85674..86639
                     /locus_tag="IWO52_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0354480.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(86738..87910)
                     /locus_tag="IWO52_000182"
     CDS             complement(86738..87910)
                     /locus_tag="IWO52_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0354481.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(87897..89042)
                     /locus_tag="IWO52_000183"
     CDS             complement(87897..89042)
                     /locus_tag="IWO52_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0354482.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(89056..89980)
                     /locus_tag="IWO52_000184"
                     /pseudo
     CDS             complement(89056..89980)
                     /locus_tag="IWO52_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            90097..91176
                     /locus_tag="IWO52_000185"
     CDS             90097..91176
                     /locus_tag="IWO52_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0354483.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            91208..91801
                     /locus_tag="IWO52_000186"
     CDS             91208..91801
                     /locus_tag="IWO52_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0354484.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(91911..92138)
                     /locus_tag="IWO52_000187"
     CDS             complement(91911..92138)
                     /locus_tag="IWO52_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0354485.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(92241..94829)
                     /locus_tag="IWO52_000188"
     CDS             complement(92241..94829)
                     /locus_tag="IWO52_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0354486.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(94849..95508)
                     /locus_tag="IWO52_000189"
     CDS             complement(94849..95508)
                     /locus_tag="IWO52_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0354487.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(95557..96060)
                     /gene="trmL"
                     /locus_tag="IWO52_000190"
     CDS             complement(95557..96060)
                     /gene="trmL"
                     /locus_tag="IWO52_000190"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0354488.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(96281..97147)
                     /locus_tag="IWO52_000191"
     CDS             complement(96281..97147)
                     /locus_tag="IWO52_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0354489.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            97256..97897
                     /locus_tag="IWO52_000192"
     CDS             97256..97897
                     /locus_tag="IWO52_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0354490.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
BASE COUNT        29272 a        20174 c        16754 g        31804 t
ORIGIN      
        1 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
       61 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
      121 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
      181 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
      241 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
      301 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
      361 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
      421 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
      481 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
      541 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
      601 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
      661 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
      721 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
      781 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
      841 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
      901 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
      961 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
     1021 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
     1081 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
     1141 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
     1201 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
     1261 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
     1321 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
     1381 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
     1441 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
     1501 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
     1561 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
     1621 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
     1681 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
     1741 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
     1801 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
     1861 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
     1921 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
     1981 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
     2041 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
     2101 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
     2161 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
     2221 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
     2281 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
     2341 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
     2401 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
     2461 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
     2521 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
     2581 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
     2641 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
     2701 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
     2761 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
     2821 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
     2881 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
     2941 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
     3001 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
     3061 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
     3121 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
     3181 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
     3241 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
     3301 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
     3361 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
     3421 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
     3481 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
     3541 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
     3601 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
     3661 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
     3721 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
     3781 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
     3841 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
     3901 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
     3961 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
     4021 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
     4081 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
     4141 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
     4201 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
     4261 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
     4321 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
     4381 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
     4441 aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca ttgtcatatg
     4501 acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
     4561 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
     4621 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
     4681 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
     4741 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
     4801 gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa gctatacagg
     4861 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
     4921 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
     4981 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
     5041 tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca tagttataac
     5101 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5161 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5221 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5281 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5341 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5401 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5461 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5521 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5581 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5641 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5701 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5761 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg taccttggat
     5821 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     5881 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     5941 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6001 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6061 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6121 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6181 gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6241 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6301 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6361 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6421 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6481 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6541 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6601 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6661 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6721 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6781 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     6841 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     6901 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     6961 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7021 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7081 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7141 ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7201 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7261 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7321 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7381 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7441 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7501 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7561 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7621 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7681 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7741 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7801 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     7861 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     7921 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     7981 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8041 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8101 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8161 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8221 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8281 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8341 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8401 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8461 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8521 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8581 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8641 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8701 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8761 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     8821 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     8881 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     8941 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9001 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9061 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9121 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9181 gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9241 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9301 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9361 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9421 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9481 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9541 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9601 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9661 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9721 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9781 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
     9841 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
     9901 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
     9961 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10021 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10081 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10141 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10201 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10261 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10321 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10381 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10441 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10501 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10561 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10621 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10681 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10741 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10801 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    10861 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    10921 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    10981 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11041 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11101 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11161 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11221 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11281 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11341 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11401 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11461 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11521 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11581 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11641 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11701 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11761 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    11821 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    11881 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    11941 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12001 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12061 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12121 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12181 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12241 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12301 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12361 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12421 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12481 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12541 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12601 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12661 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12721 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12781 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    12841 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    12901 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    12961 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13021 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13081 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13141 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13201 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13261 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13321 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13381 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13441 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13501 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13561 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13621 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13681 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13741 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13801 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    13861 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    13921 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    13981 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    14041 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    14101 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    14161 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    14221 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    14281 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    14341 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    14401 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    14461 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    14521 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    14581 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    14641 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    14701 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    14761 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    14821 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    14881 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    14941 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    15001 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    15061 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    15121 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    15181 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    15241 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    15301 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    15361 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    15421 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    15481 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    15541 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    15601 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    15661 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    15721 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    15781 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    15841 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct acactgttcg
    15901 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    15961 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    16021 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    16081 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    16141 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    16201 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    16261 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    16321 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    16381 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    16441 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    16501 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    16561 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    16621 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    16681 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    16741 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    16801 tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    16861 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    16921 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    16981 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    17041 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    17101 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    17161 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    17221 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    17281 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    17341 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    17401 attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    17461 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    17521 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    17581 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    17641 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    17701 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    17761 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    17821 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    17881 gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta ccagctgttt
    17941 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    18001 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata ggcgaaatat
    18061 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    18121 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    18181 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    18241 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    18301 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    18361 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    18421 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    18481 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    18541 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    18601 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    18661 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    18721 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    18781 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    18841 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    18901 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    18961 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    19021 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    19081 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    19141 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    19201 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    19261 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    19321 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    19381 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    19441 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    19501 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    19561 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    19621 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    19681 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    19741 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    19801 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    19861 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    19921 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    19981 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    20041 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    20101 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    20161 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    20221 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    20281 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    20341 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    20401 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    20461 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    20521 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    20581 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    20641 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    20701 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    20761 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    20821 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    20881 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    20941 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    21001 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    21061 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    21121 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    21181 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    21241 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    21301 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    21361 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    21421 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    21481 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    21541 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    21601 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    21661 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    21721 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    21781 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    21841 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    21901 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    21961 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    22021 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    22081 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    22141 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    22201 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    22261 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    22321 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    22381 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    22441 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    22501 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    22561 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    22621 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    22681 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    22741 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat ttagtctcaa
    22801 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    22861 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    22921 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    22981 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    23041 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    23101 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    23161 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    23221 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    23281 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    23341 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    23401 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    23461 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    23521 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    23581 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    23641 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    23701 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    23761 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    23821 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    23881 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    23941 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    24001 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    24061 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    24121 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    24181 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    24241 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    24301 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    24361 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    24421 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    24481 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    24541 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    24601 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    24661 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    24721 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    24781 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    24841 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    24901 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    24961 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    25021 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    25081 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    25141 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    25201 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    25261 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    25321 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    25381 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    25441 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    25501 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    25561 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    25621 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    25681 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    25741 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    25801 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    25861 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    25921 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    25981 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    26041 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    26101 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    26161 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    26221 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    26281 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    26341 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    26401 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    26461 tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca agcaatcttt
    26521 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    26581 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    26641 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    26701 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    26761 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    26821 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    26881 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    26941 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    27001 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    27061 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    27121 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    27181 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    27241 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    27301 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    27361 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    27421 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    27481 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    27541 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    27601 atataaatgt taaacttact tattttttat cgattttaat taaccaaaat attaataatg
    27661 attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac
    27721 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
    27781 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
    27841 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
    27901 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
    27961 ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac
    28021 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
    28081 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
    28141 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
    28201 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
    28261 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
    28321 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
    28381 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
    28441 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
    28501 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
    28561 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
    28621 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
    28681 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
    28741 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
    28801 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
    28861 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
    28921 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
    28981 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
    29041 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
    29101 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
    29161 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
    29221 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
    29281 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
    29341 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
    29401 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
    29461 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
    29521 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
    29581 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
    29641 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
    29701 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
    29761 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
    29821 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
    29881 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
    29941 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
    30001 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
    30061 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
    30121 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
    30181 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
    30241 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
    30301 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
    30361 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
    30421 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
    30481 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
    30541 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
    30601 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
    30661 cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
    30721 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
    30781 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
    30841 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
    30901 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
    30961 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
    31021 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
    31081 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
    31141 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
    31201 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
    31261 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
    31321 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
    31381 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
    31441 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
    31501 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
    31561 ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt
    31621 taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat
    31681 cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt
    31741 cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata
    31801 gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc
    31861 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
    31921 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
    31981 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
    32041 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
    32101 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcatgaga
    32161 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
    32221 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
    32281 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
    32341 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
    32401 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
    32461 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
    32521 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
    32581 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
    32641 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
    32701 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
    32761 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
    32821 cataagaagt ggttatctta cagctggcct gaatatcagc caaaagcgca taaaggacta
    32881 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
    32941 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
    33001 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
    33061 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
    33121 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
    33181 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
    33241 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
    33301 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
    33361 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
    33421 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
    33481 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
    33541 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
    33601 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
    33661 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
    33721 cttcaagata ccttcgctct ttttctttct gatagcccgt cggtaaaagt gtcactttgt
    33781 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
    33841 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
    33901 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
    33961 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
    34021 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
    34081 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
    34141 cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt
    34201 cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac
    34261 ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc
    34321 tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg
    34381 tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca
    34441 aagtctctta ttttaaattg aatacattat aatgatactg taaatatatc tttattatta
    34501 gtgaatggat atttactttt aatagataaa atattataac tattaaaaat aactattttc
    34561 attataatta cacacaaaaa agagcgtgag acaaaagtaa agaacacttt tgtctcacgc
    34621 tctaaacacg tataaacagc ggaagcagaa gcaactcctt cggaaataag ctgaaattca
    34681 caaaaatttg aaaagcaatt tccgtgaatt tcttcttatt tcttggagtt aaacgcttct
    34741 gttccagcct ctttttatct gtctttcact aatgatcagt tgtagataag caagttgctt
    34801 tttcttacat cataccgccc atcattgatg gatccattgc aggagctgct ggcgctgctg
    34861 gttctggttt atctgcaacg actgcttctg tcgttaataa caaggcagaa acggaagcgg
    34921 cattttgcaa ggcagaacgt gttactttag ttgggtctac gatacctgct tcaaccatgt
    34981 ttacccattc tccagtagca gcattgaaac cagtgcctag ttccacgttc ttcaatttat
    35041 caacaatcac tgatccttca taaccagcgt tttcagcgat ttgacgaatt ggttcttcta
    35101 aagcacgaac aacgatcttg atacctgttg ccacgtctcc ttctgcttct acagcagaaa
    35161 ctttgctgat aacgttcact agagctgtac caccacctga gaccattcct tcttctactg
    35221 cggcacgtgt agcattcaat gcatcttcaa tgcgtaattt taattctttc aattctgttt
    35281 cagtagctgc accaacttta acaacagcta caccacctgc taatttagct aagcgttctt
    35341 gtaatttttc gcggtcaaaa tcagaagttg tttcagcaat ttggtttttg atcaattgca
    35401 cacgagcttc gattgcttct ttttctccag aaccttctac gatcgttgta ttgtctttat
    35461 ctacaacaac tttagaagca tttcctaagt tttcgattgt tgcatctttc aattctaaac
    35521 ctagatcgtc agtaattact gtaccacctg tcaaaatagc gatatcttca agcattgctt
    35581 tacgacgatc gccgaaacca ggagctttta cagctactac gttgaatgtt ccacggattt
    35641 tgttcaatac aagagttggc aatgcttcac catctacatc gtcagcaata atcaacaatg
    35701 gacgtgattg ttgcaagatt tgttctaaca atggcagaat atcttggatg ttagagattt
    35761 ttttgtctgt gatcaagata tatgggtttt ctagaacagc ttccattttg tcgttatctg
    35821 ttaccatata ttgtgataag taaccacgat caaactgcat accttctaca acatctagtt
    35881 ctgtttcgat cccttttgat tcttcgattg tgatgacacc gtcgtttcca actttttcca
    35941 ttgcatcagc aatcaaatgt ccaactttat cagaacccga agaaacagct gctacttgtg
    36001 caatggcttc ttttgaatca acaacagttg agatattatg caattcttcg actgctgctt
    36061 ttgttgccaa ttcgatcccg cgacgaatgc ctagaggatt agcacctgca gttacgtttt
    36121 ttaaaccttc gcgaacgatt gcttgtgtca gaacagttgc tgtcgttgta ccgtctcctg
    36181 caatatcatt tgtttttgat gctacttctg atacaagttt tgcacccatg ttttcaaaat
    36241 gatcttctaa ttcgatttct tttgcgatcg tcacaccatc atttgtgatc aatggagagc
    36301 cgtaagattt ttctagtacg acatttcggc ctttaggtcc taatgttact ttcactgtat
    36361 ctgctaattt atctaccccg cgtaacatag ctgcgcgtgc gtcttctgca aatttcaatt
    36421 cttttgccat gattgattca cctcattgat tatttttgat tattttttta tcttccaaat
    36481 ttactcaaca atcgccatga tatcttttcc tgcgacgatc agatattctt ttccttcgta
    36541 ttttacttct gtaccggcgt atttttcgaa cataacttgg tcgcctgctt ttacagcggc
    36601 aggaactttt tcaccgtttt ctaacaaacg gccttcaccg actgcaacaa ctgtcccagt
    36661 ttggggtttc tctttagcag cagaagctaa aacgattcct ccgacagttt tttcttcttc
    36721 ttttgcgact tcgataatga cgcgatcacc taatggtttt aacacgataa atccctccga
    36781 taactctttt tttaatctgt tagcactctt aaccttcgag tgctaaccta catttttaat
    36841 aatactcatt ctccctcttt tttgcaagct tttaaatcaa agaaaagaaa ttttattctg
    36901 acagctgctt attgaaatta tgatataatt gaaatcaatc gatataagct tgccttgcat
    36961 gcttgttatc ccttaaaagg aggaattaca tgtctttaaa aaaatatagc ttcctcagca
    37021 ttttttgcta tggtctaatt tttatctcac ccttgatcct agcttcgata ggacttgtaa
    37081 aatctacgtc tgaattgatc acagcaactg cagtcatgta tatactaggc gccgttgtcc
    37141 tagctatttt ttattttaag caacgtgaac ccttagcgat cgaatcagcg gtaaaaaaag
    37201 cttctgctcc aaagattgtt atttatggat tggttggaat ctttgtcgca ttgattctgc
    37261 aaagcatcgc tgtcatgtta gaatctgtat tgtttggcaa agcaacccct tccgagaata
    37321 cacaaaatat catccagatg atcatggaag cacccgcttt tattttggcg acaactgttg
    37381 ctggacccat catggaagag ttcgtattcc gccgcagtat actggggatc atcagtcgat
    37441 attcaaattt ttgggtcgga gcagtgatca gctctttgct gtttgcgttt gctcataatg
    37501 atggtcatct gctaatctac tttttcctag gtttcttctt cagcctagag tacaaagcaa
    37561 ctggtcggat ctggacatcg atgatcacac acgtaggaat gaacacatta gttgttcttg
    37621 ttcaattagc tgtccaaaaa gggctagtat aaacatgact ataaatactc ctgtcatcct
    37681 agattggtga ttttcccaat ctaaagcgac aggagtattt ttgtttttta gtcgttcatc
    37741 ttttctgctg ctccatcatt ttttgtctgt ctttcctttt tgcagtttgc agaatctatt
    37801 tttttcaaaa atgggaaata aatgaaaaaa ctaattgaca acacgatacc ttgtaaaatc
    37861 gctgcaggca ctccgcctac taagaaaccc gagaggatcg gcggtgttgt ccaagggact
    37921 tggactgctg taaataaagg aacgaatcca aaatataaag cggaataggt aatcaatcct
    37981 gaaacgaccg gtgcgaaaat aaaaggaaca gccatcatcg ggttcatcac gatcggtgtg
    38041 gaaaaaataa ttgattcatt gatgttgaaa aaggctggta aaagagcaag atttcctaat
    38101 tctctgtatt tcttcgattt cgcaaaaacc gtcataaaaa atacagcccc taacgtcaat
    38161 ccagcacccg taaccgtcat gaactgatcg agaaattgct gagtaacgat atgtccgcca
    38221 tttgccaccg tcaactcttg tcctgaggct aagatttctg catttgctaa tgaattcgct
    38281 tgcaagacgg gactaaccaa cccattgacc atcgcagatc catgaagacc aaataaccat
    38341 aaaaacggaa caaaaaaacc taataaaagc acaccaaaca acgtgtctat gaacgattga
    38401 atgggaagct ggacatatgt ccatactcct tgaacaacag tcattctgaa caaatgttga
    38461 aaaaccgctg aaccgataag cgtaaaagtg actataacaa tgattggaat caatgaagta
    38521 aaagactgcc aaacattttc aggtatcttc tctctttgct tccacccttt cgcaagtctt
    38581 actaaacgtg catagcccca tccgatgatg agcccagttg taactgctcc tagtatccct
    38641 tcagcacctg tccagacagt atctatctga gtatttagtg gctgaataag cagtagataa
    38701 gcagacaagg ctgttatgcc gactggtaag cgatcgatgt cttcttcttt tgcataagcg
    38761 tatgcaatgg caaaaacgat ccaaaaggct atcacatcaa atgtagcctg gtatacttgt
    38821 gtaaaataca tagaaaatcc tgttttgtct atccactgag cgatgtaatc cacaggaaaa
    38881 tgagcaaata agaaaaacag cgagccaaca ataattattg ggatcgtgta agtcattcca
    38941 ttacgcaaag cagttaaggg tctaagattc acaacagatg ttatcattgg taaaaaggca
    39001 cgatctaacc actctttcat ttcttatctc cttcaacttc tatttttatc aatcagtttt
    39061 cttaatttaa ctatacccaa tgatcacgaa ccatgaaagg aatgcgcact gagcttttca
    39121 aaaatcggat tctttgacga atattcagca actgcttaca atggacagga ataaaaaagg
    39181 aggtttttta tgaaaattgc actcattgga gctactggcc atgcaggttc atttatttta
    39241 gacgaagcat tgtccagaga tttagacgtg acggcaatcg tgcgtcatcc agaaaaattg
    39301 ccatcagatg ttccgtttat acaaaaggat ttatttgagt tgacaactac tgatcttgca
    39361 tcttttgatg ttgtcgttga tgctttcaac gctcccaaag gaaaagaaga aatgcatcaa
    39421 acttctttgt cccatttgat tgagatgctt gaaggaaccg atacacgctt aattgtcgtt
    39481 gggggtgcct cttcgttatt cttagataca gaaaagacga ctcgtatgat tgaccaagta
    39541 cctaaaaatg cacccttcta tccaacagct ttcaatatgt acgaaggatt gttaaaattg
    39601 aaacaatcta aaaatctaca ttggacttat atcagtccag cttctttatt tgatccacat
    39661 ggcaaacgta caggtactta tttgttaagt gatgattatt tacaaagaaa cgctgctagc
    39721 gacagtacga tcagcatggc agattacgcg attgcattag tagatgagat actggatgag
    39781 aaacatgaaa agcagcacat cagtgtagtc agtcgataat acggttattt ttttaatatg
    39841 ctaaaaaaat aaaaatagaa gataaaacca accctgattg attttatctt ctgcttttta
    39901 tatttttatt gattgaactg tttaaatcga aaatagctca gttgcttcat ctggatctgt
    39961 atcatagatt tcaaaatcat gtccaacgcc aaaatctttt tctttcaaaa cattttccat
    40021 tgttaaatga gcaagttctt tcatattgtt ttccttgcta agatggccaa gatagattcg
    40081 tttggtccga ttaccgatca catcagtcat gacaagtgca ccgtcttcgt tggaaagatg
    40141 tcctctatct cctaaaatac gctgttttaa attccacgga taaggtccca tgcgtaacat
    40201 ctcaagatca tgattacttt ccatcaaata accatcggca ttttcaatga cacctcgcat
    40261 ataatcactg caatatccgg tgtccgtcaa catgacgaat gaccgatttt ctttatggaa
    40321 acgataaaat tgcggagccg ctgcgtcatg ggagacgccg aaactctcaa tatcgatatc
    40381 gccgaatgtt ctgactttcc ccatctcaaa tagatgtttt tgttccactg gtacatttcc
    40441 aatcagtggt tccatcgctt cccaagtctg ctgattggca aaaacatcca aatgatactt
    40501 acgagccaag acacccacgc catgtatatg atctcgatgt tcatgcgtca caagtattcc
    40561 atctaattgg tccggtgtcc gatcaatttg agccatcaag gaagtgattt ttttaccgct
    40621 aagtcctgca tcgaccaaaa gccgtttttt ttcactttcg atataaagtg aattacccga
    40681 acttccactt gctaggatac tgattttaaa agctggtttt tcttcagcca tttgactcct
    40741 acttcccttc ttactttgta aacttttttc attatactcg cttttttatt gatcttccac
    40801 ttttggtaca gtattgtttg tcacgatcgt attgctgaaa gcgttgacgc tctcaatttg
    40861 gaccacttta tcgcttgttt cgatcgctac atgccaaaca ggaacataca catttttttc
    40921 ccggactttc agaatacagg aataagctag ctgacgccat aaaatttttg atcctcttgg
    40981 tattttgtta ttgatgtaca atgtattgat tgcttcctct tccgtataca gttccgtctt
    41041 ttcgcgcagc tgctcgatat cagataaatg cgtctgcatg tatttcgtga tgctataatc
    41101 attatcgttt ttttctaaag aaagaacgat ttttgctgta tcatcattga atgggatgcc
    41161 ttcatactct tggcttgctt ggatttctag tggttcttct tctaaatgag aaaagttttt
    41221 cgtgtacaca taatcattac cgaataaaat attgtctttg ttagataaaa aatcttccaa
    41281 ggaactacta attttatctg gatcgatcac gttcttttca gcatctgaac tggataaaga
    41341 tttcgtcaaa acatcaccgt ctaaaaacac accacttgcc agcaagccgc ttttaccagc
    41401 cttgcgataa ttcgctaatg aagtgctaag attggtctgc tccgcgctaa gataatagcc
    41461 ttgcttgttc tcagacgaaa tcttccccgt gtaagtgata tcgtcgctag ccagacgttg
    41521 ttggattgat tcagtctgat tagagcgaat gacattgttt tcttggtata ccgcgtcatg
    41581 ataactgctg aataaaaaca cattcaaccc taaaaaagcg acgaaaaata tccattcgat
    41641 ttttttgaaa tccaattaat tcgcctccaa tccaggtaat ttttccagaa ggtcgttagc
    41701 tgaataccag ttatctttat atttcacata ccattcaggt aagagatcaa cgacacgatt
    41761 cgtttctttg acattttgcc atgtatagcc aacgatcact gaaccaatct tgttgctatc
    41821 tgctccctta gcttttaact gctggatgat ttcattcgta cttgcaagtt ctacttcttc
    41881 gtctgaaggt atcggcactt gaatcgtatt caaactggtt tggatattca cttgcacaga
    41941 accattcgtt tcaccgccaa cttccacacc aatctgtcct ctgctgtctg tcccaaaaac
    42001 cgggaagcct tccacgaata tccgataatc gatcgtattg tcactgcggt caaaatatct
    42061 gaggttccct aaagaactgc ctaatttgct aatatagggg aaactttcag aaaatacact
    42121 agcattttct tttttctctg gcagctctcc acgaaaatca actacttgcg tttccgaatg
    42181 gatagacatt gtttcattcc caccgtaaaa aatcaattca ttgctttcat cattattttt
    42241 cacgctatct ggtttctgga aaaaagcatt tctaaaaagt gtatagggtt gctgtgacag
    42301 gatataacta tacttcttca gtttgatcga atgtttcgta gcatattgcg tgtcgatcgt
    42361 cgatggttct gttgtcattt ctgtccattt tgtgtctttt gctgttaatt ctttattgat
    42421 atctgtccaa ttcaacgaaa tatttgcttc atatacgaga tggttcgaat aattcaaaaa
    42481 tcggattttt ttatttcctt gatcgaattg tattctagta aaatacatat tttcgctttc
    42541 ttttgccaag ttcgacacat ctaaatctaa atcaaatgct tccttgtatt ctcttagcaa
    42601 aaaaggcgca ttataggcca actcaaaacc agagttcaga tttttcacct gttcaaactg
    42661 tgatttgtct cctgaaacta cttcagaaag ctttccaaat cttgctttat cgatgatcaa
    42721 ttgcaatcgg tacacgagat tttcactgtt ggtttcttgg atcattcccg aatggtgcca
    42781 tgtaacatga agcggaagaa agacttcgga agtttttcga ttgctttgag actcgatcat
    42841 ttgagtgttt gtctcatcta cgttgattga actttttcct gctgggctca gccagattgc
    42901 ataagagaag tatatgctta acgcaaccat caaaatgagt cctatacgaa cgactttttc
    42961 agagattctc attcccacca ttcctcctca taaggttcat atggcagcga gatataaaaa
    43021 gtcgaccctt ttccttcctt gctctctgcc caaatagcgc ctcgatgcgc cttaatcact
    43081 tctctagaaa tagctaatcc aagtcctgtc ccaccttgtg ctcttgcacg tgctttatct
    43141 actcgataga accgctcgaa cactcggttc agatcttttt taggaatccc caatccttga
    43201 tcggtgatac ttaaaatcac gttattatgc gtttctaata agcgacaagt aatcgttccc
    43261 ccatctggtg aatacttgat cgcgttattc atgatattat cgactacttg gatgatttta
    43321 tctgtatcta cttcgaccca aagatctctt tgcgtaaatt cacgacggat cttataattt
    43381 ttttcctgat ttcccaccat catatcaaac cggtcaagaa caaaattcac taactcgttg
    43441 aaattgacat attctaattg aagctgggta ttacctgtat ccatgcgaga tagatttaag
    43501 aggtcattga tcattcgaat cattcggtct gtttcatcta gcgttacctt caaaaaattt
    43561 ggtgctactt cctcgtcttt ccacgcacct tcgctcaatg cttcgatata actacgcata
    43621 cttgtcaacg gtgtacgaag ttcatgggaa acattagaga cgaattcacg acgttcccgt
    43681 tctgtttttt cttgttctgt cacatcatgc aagacagcta ctaatccgct gataaaacct
    43741 gattcgcgac ggatcataga aaagtctact cgcaagatca tctgatcact ttccaatgta
    43801 ctggttgaac ggtcaagtac catttcgttt ggatcttcta ataatttccg caacgtatat
    43861 tcttcttcga tatctaaaag tgtcaaaatc gattgtccga ttgcttcctc gtttttgata
    43921 tctagcaagg aaacagccat atcattgatc gtaatgactt tacccctgcg atctgtcgca
    43981 atgacgccgt ctgtcatatg ggatagcacg ctatccaaac gattgcgttc agcttccatt
    44041 gtatcttgag cttcttcaat acgttcagct aactgattga aggtatcagc cagctgtcca
    44101 agttcatctt gtccatgcac tttgactttc cgactgtagt ctcctctagc gattcgaatg
    44161 gcttgttctc tcatttcacc gatcggctgt gtaatcgaac gagcaactaa aacagatacg
    44221 atcattgaaa taaatgccgc aattagtgag gcagtaaaga aaatgactgc tgtgttattg
    44281 atttccgaat atttttgttc gatattactt ttgacgtaaa gagccccgat aacggcatcg
    44341 ccggtcgggg acaggatcgg ttgaacattg atattcaccc gtttatcgtc gttatctaaa
    44401 gcaacataac gtttagtcgt gaaatcattg atatcaacat aatcattttt ctttcccaca
    44461 gcgctttgtt cattgacatc ggttgttcct cggatgatcc ctttttcgtc aacgacccgt
    44521 atttcgatga tatcgctagt cgcactgttt tcaataaatc ttttgatatt cgcatttacc
    44581 tcttcatcat ctgcattatc ttttcgattc aattccgggc tgattgttgt tgctaatgat
    44641 tccaccgttt gattcatatc tttaataaat gtattgatcg tggaacgttc taaaccacgt
    44701 ataaagtagg caccaataat ttcgatcgag atcaataaaa tcagaataaa ggtaatagcg
    44761 atcttaaaat tgactgatcg aaaaaagcgg acttttcttt tcatccaatt actcctgttc
    44821 tgggttacgc aaatagtaac cgacaccacg acgtgtcact aaataatttg gatggcttgg
    44881 attatcttcg atcttttcac gcaaacgacg tacagttaca tccactgttc gtacatctcc
    44941 aaaataatca taaccccaaa ccgtttgtaa taaatgttct cttgtcatca cttgtccaag
    45001 gtgcttcgcc aagtaaaaga gtagttcgaa ttcacgatgt gttaactcga ttgtttctcc
    45061 tcgtttcgtc accatgtaag cttctggatg gatcgtcaaa tcaccaatcg tcaattccgc
    45121 aggtgctgcc tcttctggtt ccttcgcagc cgtggcaccg cgacgaagat ttgctttgac
    45181 acgtgcaact aattctcggt tggaaaatgg cttggtcacg taatcatcgg cgcctaactc
    45241 taagcctagg actttatcaa tttcagaatc tttggctgtt accataatga tcggcatatc
    45301 ataagttttg cgtacctcac gagcaacttc taagccgtcc attttaggta gcatcaaatc
    45361 aagtaaaatc aaatcaggtt ctacctcagc aactttttcc aaggcttctt ccccatcata
    45421 tgcagtataa acgtcatagc cttctttggc taaattgaat ttgacgatat ccgaaatcgg
    45481 cttctcatca tcgacaacta aaactttttt cataaagagc cacctcttat atattattgt
    45541 cataccttct cattataccg tatttctgct tttgtttcat ctttgcctat aaaacaaaag
    45601 agaaaattaa aatgaaagtg caaaatactt tattattttt tgttctaact tcaataaaaa
    45661 aggaaaacac tgattttcac ctcagtgttt cctttttttg atttatcctt ctaaaacttg
    45721 tacaccgaca cccaataatc ccggaccagt gtggactact aatgccggag aaatctggcc
    45781 aaagtagatt tggtttgctt gtgggaactc tcttttaagt ctcgcttcca tttcaattgc
    45841 ttcttctttc gcatccccat gggcaacagc taagttgaag acctttgcat taccgatttt
    45901 ttctttgacg taagcaaccg ttttatccaa acttttttta cgtcccctag cttttcctac
    45961 tgtgtaataa atcccttctt cattacatga gatgattggg ttaagcttta aagcggttcc
    46021 tacaatagaa gcaactaaac cgatccgtcc acctttttga agatattcca atgtcgcaac
    46081 attaaagaaa acttttgttc tttcgactaa ctcattcaga ttactgatca gttgatccca
    46141 ttctactcct tcttccagcc attttgctgc ttggatcgca gaaaaacctg caccaatacc
    46201 aatatttttc gtatctagta catatgcttc caattcttct tgctgttgtg caattaaacg
    46261 aaccacatta tatgtcccgc tcaatccact ggaaattgtt accgcaagta ccttttcata
    46321 accgtctttt ttgatttgct caaagatttc tgtgatcgac tcaccatccg gcaaagatgt
    46381 accagggatt tcctctggca aacgttgata gatctcttca ggggtaatgt caattttatc
    46441 tgtatatacc tgatctttat aaataatttt caatgggatc atatacatct cgtatttttt
    46501 tatcaattct tctggaacat ccgtacctga atctactaaa attgcgattt tattgttttt
    46561 catttgtttt ctcctctttt aaatattcgg tttctagaac aatcgttttt tccgcaagaa
    46621 gtttcattgc aaacgataaa gttgccgctc ttacagcaat atattctaca ggggttgcct
    46681 ggtcgaattt ttgcggatga gatttacctt gtgctaattg gctgaccatc cacacagctg
    46741 cttcttgttc atcgcaaaat agattatagg cttcacgaat tcctactgtt tttccttgga
    46801 acaatatccc ttctttgatt tcaggaatag tcaatacttg tttcaacgta gtgattgcaa
    46861 gtaaaaatgc tacgtgttct ttgttatatc tttttttaac tggtgctggt atcaaaccta
    46921 gtttaacgta attgtttacc atagaagagg taagcaatgg atgcttttct ccttgtatca
    46981 caggtgaaag ataacgatca actaacgttc tgacttggtc catataaagt tctaagtcag
    47041 ggagttcttc ccagtgaggc agacggaatg tttctagttc cttcccccac tccattaatt
    47101 gctgacgcat tttttccatc ataaccgctc cttttttcat ttcataaaca tgatatcaga
    47161 cttatgataa acattcaatc tagttgcttt aactaggtga tttgattagc gggattccat
    47221 gaaaacaaaa aacaagaagg ctaagccttc ttgtctttta tcgttaagaa caggagaccc
    47281 gccggggaga cggcgggaaa ggagtaaaaa atgaaaaaag tgttagggtt tgttctcttg
    47341 caagaacttt gtttgttggt atgcttatat aatagcgagc aaatgtgaat aatctatgaa
    47401 tgatttccta taaggaagta aagatttttc tgattttctt taaatgtagt cttgcatttt
    47461 aaaattctta gtgctactat tattaagtac tttttgtacg ggccgttagc tcagttggta
    47521 gagcagctga ctcttaatca gcgggtcgcg ggttcgagcc cctcacggcc cattgggtgc
    47581 caaacccacg gaacaggtaa atgttggtgc gaaagcattt cgaaaacatt tacgctgttc
    47641 tttttttatt tttcaaaata actagggctt tttattttta actcaaagtc cactgtagcc
    47701 ctttggactt tgagttaaaa aagaagcata attatgaaca gtaaagtttc taatataaca
    47761 ataattcgag aagctttgca aaatcatgct gccaatgaac tctctaaaca tataggatta
    47821 aatctaagta ctataaaagg ttaaaaagtg aagaatattt aatagaaaaa ctaaatttac
    47881 ttgctgcttt ccccttttcc attaatacaa caatcagaga gtgtcttttt ttcgaaaaaa
    47941 ttagaataaa taaaagacac gtagtttttt tatctacatg tcttccatcg actacaagtt
    48001 atcatttctc aaaacgtatt tagcttttaa ttgattaatc gtttcacgtt gtctacacat
    48061 tgcctaatta agattttctt attagaagca tcaatcccgc aataatacct aaaactccag
    48121 gaattaccca caataatgtt aaaagaaaaa atggaataat agataaaatg agtaaactaa
    48181 taccatagac cactgttttt tgattttttc ttactaaaaa acctattatg aaaatcatta
    48241 tcgaaaaaac tagggtaatt cctccaaata aaacaacatt cccgcttaga ttgactaaca
    48301 tatctatatc cttactggtt agttctgtag tatttaaagt agttgaatca atagaagata
    48361 gaaacatacc acccatcaat gctaaaagac tacataaaat ccctgttatt ccactaatta
    48421 aaatcaaaat aaactctgtt tttctcttat tcatttaatt ctctccagat taagtgtttt
    48481 agaattagaa attttttgac catagttaat agcttctaat gaacgaataa caaccactga
    48541 cgtgttagaa aaataatccc caatatgttg tttcttgggc gtgaaagcag ccaccaaata
    48601 cccaatttga aatacaaaat tttttaatat aaacctacat atacactcac gaataaagat
    48661 agtttgccaa cttagctctt tttcctcaaa gctcactact cttatcccaa aaatcatttt
    48721 cccaattgtt tgtcctgaat tcagcttagt caaaagtaca aaataggtta aataaatagc
    48781 taatgacgct ataccataaa aagtaataat ggatttgtca gattccagtc ccatgatttt
    48841 gtaaaagaga cctatagtag catgggtgat gatgctaata caaattaaat caataagaaa
    48901 agcaaaaaat cgtaaccaaa atcccgcaaa tacataatta ggcaaagttt caatgccact
    48961 cttcttattc attttatagc cctccatagt aatacattgg ttttggagta tcagaaaatc
    49021 caagtttttc aaaaactgaa attacacgat ctgatgtagt tgcctttagc ccttgccatt
    49081 cagcaatctt attacctaac caagaagaag caaagccagt tgtgtcattt ttatattcta
    49141 taaccgtcgc atcagttaat ttcttctctt tttttaggga atctaatgca tcttcagagt
    49201 aaccaatttg atccaccaac ccattttcta ctgcttggtt accatcataa attcttccat
    49261 ccgcaagttc tttcactgca ttagtatcca tatttcttcc ttttgctaca acattgacaa
    49321 aacgatcata agcactcatc acaaattcct gtataacttt attctcttcc tctgaaggtt
    49381 tgtgttgagg tgacatcata tcttttaatg caccactttt atacgtcgca tcagttacgc
    49441 caagtttttc taataagcca gaataattta aaccagaaat aatgacacca attgatccag
    49501 ttgttgtttc ttccgtagca aatattttat ctgaaccagc agaaatgtag taccctccac
    49561 ttgccgctgt atttttaaat gcagtataga taggaatatc tagcttttta atttttgcca
    49621 tttctttagc aatctcagca ctttcatata ttcctccccc aggtgaattt acttctaata
    49681 acactccctt aaccgctttg tcttcttgaa ttttctttag ctgtgttaaa aaattctgat
    49741 ggttatattg ttccctacta aaaagattgc tttcacctgt atctgcaata actccattga
    49801 cggatagttt gacaatcttt ttatttgatg cgccttcttc taacgtttct tctgtgattt
    49861 cgttagatcc gtaaaaaatc ttatttattc ctgttagggt tgtatttgat gcttcctctt
    49921 tatcttttcc tggaattagc gcgacgataa tggatagtaa caataatccg cacgctactc
    49981 ccacagcaat ccatcttctt ttattcatat aaactcctcc tatattctta gtaaaaataa
    50041 ttatttttta tttagaaaca catcgacaat ttgttgccaa tatgtttttt ctcctacaat
    50101 taaagacaac tctccagtta atccataacg aaacggaaac gattgagttg tttttaacgt
    50161 tcctttcact gcataaaaaa cacctttctc agtggcagta gaattttctg aaatttcatc
    50221 taaagttccg tcgatcgtaa tcggagcccc acctttttga tccaatttaa aatgaacctt
    50281 catcccttct ttaatatagg tactttcatt cgcaggtaaa agtgctgtaa atttcatttt
    50341 tgatttttcg tggatcggat aaatttctgc aagcactgtt cctttggcta gttctgcttg
    50401 tccctttgtt tcttcattta aatgaaccgt tccagataca ggagctttta gtattccttt
    50461 tgaaagttga tcgtccaaac ttttgataac tgactggttc ttctcttgtt tttcatttag
    50521 ctctgtaact gtttgtttta cagttgttat tgcttgttct agctcttgct ttcctttttc
    50581 cgtttgacta attctgtcat tctcagaagt agttggttta actaatttag ctttttctga
    50641 ctgatactgt tctacttctt tttttagttg ctcaatttgt tcatttatgc ttgctgaaat
    50701 tgtcagtttc acttgatttt tttgatcatc agaaacatta tttagttgtt cttgccaatt
    50761 ctcatagttt gccatcattt cttttgaaaa atcttttaat gattgattat tactccaagc
    50821 tacctgtact tgttgccatt cttgtaattt actatctgaa ctctcaattt gtttttctag
    50881 ttgtcttttt gtcttttcgt atacttctaa ttgcttttga tcatttaatt cactttgctt
    50941 caaagcgtag cggagtgatt ctttttctga caacatgctc tttaattgat tactgtagcc
    51001 aaaactgtca tccgttgaaa acaaattttt tcctttactt atactttcaa taaatagctg
    51061 agccatcttt atttgttgag taatacgttg gttttcttgt tctatctgag ccttttcatt
    51121 ctctattaaa gaacaatcca atctaaccaa cacatcgtcc ttatgaacaa attgattttc
    51181 ttttaggaag ttttccatta ttttagcttc taatggcact tgtaacttct ctattttttc
    51241 ggggttcaat tgagccgttg tccgaataac aacctctttt ttagcaaagg ttaaaaacaa
    51301 tccaataata cataaaaaaa gaattacagg gtataacacc caacggtaga agctatgatg
    51361 ttgtttagta tagatactag aatgttcgaa ccaattatta ttctgcataa attgtttccc
    51421 tctcctagat ttcccataat ttttgataga taccatcggc aaaacgaagt tcatcgtgcg
    51481 ttcctttttc taccaaatgc ccttcatgga gaactaaaat ttgatcacat gcttttgcta
    51541 ttggtaaatg atgcgcaata aaaataatcg ttttttcttt taaattgagg agataattca
    51601 taattgcatg ttctaacaag gtatctaatc cactagttgc ttcatctaaa attaatatgt
    51661 ccggattttt taataaagcc cgtgctaagg ctaatcgttg tcgttgccct cctgaaagat
    51721 tatttccacc ttcttccaac aatgtctcgt aacgtaatgg ctgttgatta ataaattcat
    51781 ttagttggac tatctcacaa acttctatta tttgttcgaa acttggtggg ctatctaaac
    51841 cgaatataag attctcaatg attgttccac taaagaaaaa tgactcttga ggcaaatatg
    51901 tcactcgatc gcgtaatatc tgaaaaggaa tgtctaagtg attaatgttt ccataatgga
    51961 tcgatccttc tgatgcttca taaaaacgaa caaataactt tgcaagagtt gattttccag
    52021 aaccactaac tccaactata gccgttttac tgtgcgaagg aattgtacat gaaatttcgt
    52081 tcaacgttgg ggccttcatg ctatacgaga atgaaacgtt ttttacagtg atatcttgtt
    52141 gaaataattg ttcagaaagc atatattcaa aagactggtt cgatttttct gaatcaatcg
    52201 cgaaaatttc atttaaacga ttatttgcaa cttgagccgt ttgcattttc acttgtaaat
    52261 taataatgtt ttgtaaagga tctgtcaaaa atactagtaa agcattgtat gtgataagtt
    52321 gacctagact aattctatta tccataacaa aataagaacc tagccataaa attaatgcac
    52381 tgctaattaa ttgaatcaaa tgtttaattc cttgttgaat attatcaagc gtaaccgtac
    52441 gaaatgattt tttcattaat ttaacaaatt catggtctat acggttatat actttctctt
    52501 caccgctata tgctttaatt gtttctattc ctttaagact ttctatgata ctagaattta
    52561 cagctgcacc ggctgccatt tcttccctgt tcgatttttc atagcttttc acgaaagcga
    52621 atattgcaac tacataaaaa ggcaaagagc atagagtgat taaaaacaac gtactattct
    52681 gaatagctaa cgtaacccca acaagcaaca ccataccaat atctaaaaat attgataacg
    52741 tagcacttgc taaagcatca ataatcttac tggcatcaag aaaacgagaa ataatttccc
    52801 ctgattttct cgtagcaaaa aaattcattg gtaaagttaa cacatgtttg aaatactgaa
    52861 gcataattgc catactcatt cgctgtccta aaataataag aagataacta cgactatatt
    52921 caaacaatac ccgaaataaa tagacagcga tcaagccaat ggaaattata tttaaagttg
    52981 aacgtgcttg attagggatg aaaaaatcaa gaataccttg aaaataatat gagcttccta
    53041 tgccaaaaaa cgtaatgaaa atcgatgcta aaacgatgtg gaatactaat cctttttgtt
    53101 tccaaattat aggcaaaaaa gaacttaatc cagctacttt ttctttaata ggaatataag
    53161 tttctctagg cataggtaat aaaagaatac ctgtccattc tttttgaaac gcttcaatcg
    53221 tttttctatt ttttccatta gcgggatctg caatccatag tacgtttccc ttcgttttat
    53281 aaattacaac gtagtgcata tagctgttat caaccaatat atgtgcaatt aatggtaaga
    53341 taagatctct ttctttccag atctcactat ctgcttgaat agccatacaa tcaaaagaca
    53401 atttttcaaa tgtctttttc aatccaaatg ctgaagtacc ctctaaatca gtcccagata
    53461 ggtcccgtaa tttatgaatc gggatatcag ttttaaaata ttttaaaatc atcgaaaggc
    53521 aagcaacacc acaatctttt tcatcttgtt gctgcacaaa aggaaatgat tttatcactt
    53581 atctaacaca actccttcta aaaaattttt atagaaaaat tctcaatata gctttaatac
    53641 agtgaaaata ttgtctcata attccacaaa aactttgaaa acagttcttg aacactaact
    53701 ttttcattcc tgaatcaaat ttattttgga aattttacta ttcaagaata aacttgataa
    53761 gtttcaggaa gatttttact gttaatcaaa tacgtatgtt acagtagcac cgtaaaaaat
    53821 attatttaaa gaagcgtgtt taaacatgca aaatgtaaag gaactaagta ttgtggaaat
    53881 gcacaaactg ttggtggagg caataatgct tggggaaaat tggggcaagt tgtaggtgga
    53941 cttaccacag gggcagtagg tggtgcaggt ttaggaacag caatttgcgg accagcttgc
    54001 ggtgttgttg gagggttata tggagctgtc gctggaggtg ctgcagctgg ctgggatgct
    54061 cgaaaaaaat aaaaaaggag aataagtagt atgcatatta aaaacacaaa aacaacattt
    54121 atattatctt cagaagagtt gaaaaatatt caaggtggga gtgccgtggg agttttaggc
    54181 actacttttt ctggggcaac agcaggagta aaactttgtt cagccggagg accttatgca
    54241 atagctgctt gtggtgtagg tggagcttta ttaggtgctg gattcagcat gtggaccggt
    54301 gcgtaaacaa ttaaaaaata tcaaacctca agattggttt gtgtcgatta cgaacatagg
    54361 tatgttcttt atcttttcaa aagtttggaa tggacagaac tttttactaa agcttcttgt
    54421 gctagttttt ataattatat taaattttta tcttttatta aagtcagata aaaattcttt
    54481 ggaaatgtaa tggtagagat atttttacaa aaggacttgc agcgctgtag ttggtgctgc
    54541 aggaggacct atttcggccg ttacaggagg tattggtaat gtacttgtcg gatgtgcatt
    54601 aaagcctgtt cctgcaagtt aaatttataa aaacaggaaa gtaattatct caaatcctat
    54661 caagtacaat cataggattg cacttgacag gacaaatttt ttaactaaaa aatgaaaaac
    54721 actttctaaa aattagtaga atttgccaaa tctttcgcat cttccaccat acatttaatt
    54781 ttaattcccc tttatggtac ttaataaaaa caagaaaaga actattcatt tggccctttt
    54841 ttggttccag accatagaaa tattcctcca gtaactaata ttgctgttat gcacgtaact
    54901 aataacagct ataaaaattg gtacaacttt aataggattc agaaaaattt gcactagtga
    54961 aggaatctat tttattagaa aaatgcaatc tccctaaaga aaaaaccact aaacagtgct
    55021 ttctttcaac ttctccttaa ttttttgtgc tttccctaaa gtaaagtttc tggccaactc
    55081 tgaatcaaaa tatagtgaac gttttttaaa atctatttct ttaacgttaa agatatttac
    55141 taaacacgct cgactaattc ttgttaacat aggatatttc ttttcaagct cactaattct
    55201 tccataaaat tcgtactgcc catctttagt acacaatgat aatctatgtg gtaattttga
    55261 agcttctaca aaataaatgt catttatatc aaaagtaaat gtttgagaac ctatggaaaa
    55321 aacaaatgct tgattcgtaa cctgttttgt tgcatctatc ctttcttgcg ctaataacaa
    55381 taactcaaca atctcgtttc gatactcatc tagtgtttga tcttttgtta caaatcctaa
    55441 cgtttcaact cttcttttta ttgttacggg taacatctca tcatgagtag ttgtaaaaat
    55501 gatttttgct tgaacatcat attttctgat tacttctgct aattctatac cgttaacttc
    55561 atgatttaaa tcaatatcta agaaatatat tccttgcttt ggtcggaatt gtttgaggta
    55621 ttttttaact tctaacggac tttgtgtttt caatactatc ttaaatactt tatcgtgaaa
    55681 aagaatgaaa ttatcaataa ttcgttctaa ctgatttaat tgtataattt gatcttcaca
    55741 taatattatt ggataactca taaatcctcc ttccctcaaa acaacaaaat aacttgagta
    55801 gtaaattgat acggatcgtt ttgatagttc acaaacatat tgggattctt tttattgatt
    55861 tcctctatac tagataaacc taacccttga tgtccctttt ttgttgttat tccttttttc
    55921 attagagtat ctatagagag tagtatctct ctacaactat tcgatatcga aatttctatc
    55981 tgtttatcat ctcggtaaat agctagagac agttctctat cttgactctc tatagatgct
    56041 tcaattgcat tatcgagaat aattccaagc accctaatac agtcaaatat tggaacagga
    56101 acttcaataa tagtacttgg acattcgaat ttacaacaaa tcttattgtt atttgcttgg
    56161 tatagcttcg ctatcaacaa acttttcaaa tattcatttt ttatattttt taaataacga
    56221 tattcaaact tattggttag ataagaatta gaatagatct ctaattcttt tatttctttt
    56281 tccaatatcg aatcatggtt ttgattagcg atttctttta aactcagcaa taaattttta
    56341 tagtcatgtc taaatctggc aaatttttct tgatcttttt ccagtgaatc agtatatttt
    56401 tttagataaa ccaattcttt tcttttcaat tgctgttcat attgttctcg ttgcctaata
    56461 gttattctaa taaataaaaa tagaacaaca aacatttgaa tgattaaaaa tattaagaca
    56521 ccaaacacaa aatgatcaaa tacttgatat tgatgagcaa cataagaaac aaataaacca
    56581 attgtaagta aataaatcat gaatatagca gtaagtttag aactggactg ttcaacaatc
    56641 ctatttatag ctaattttct atataaccaa ataaacatca gcaaaaacaa agaatcaagc
    56701 attatctgga atactacata accataccct tttatccctt caaatgaaaa catatacgtc
    56761 ataaccatgg aagtaataat agatctgaaa tcatcaataa gcatacataa tagtaattta
    56821 tttaaaagca ctatattagt tttcttcttt tcctttaata taaaataact tccgattata
    56881 ggtatagctg cggaaacatc agtaaataat tcaacaaaaa aagtgagtgg tatcaataaa
    56941 catacaaaaa gatattttct tttgttaaga tcagtattag tcatataaaa taagagaaaa
    57001 aagaaaaagt ttcccacgaa cattcttaat agataagctt ttgtatcgat agtaatcaag
    57061 agatcccctc ctgattttag gatatcataa aaaaaatttt agttacactt tttaaataat
    57121 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
    57181 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
    57241 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
    57301 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
    57361 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
    57421 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
    57481 aaaatataga taattcacca atctatttat taaaggaccg ggatctaatt catatttttg
    57541 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
    57601 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
    57661 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
    57721 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
    57781 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
    57841 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
    57901 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
    57961 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
    58021 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
    58081 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttggacgt ttcgaagatg
    58141 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
    58201 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
    58261 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
    58321 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
    58381 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
    58441 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
    58501 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
    58561 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
    58621 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
    58681 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
    58741 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
    58801 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
    58861 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
    58921 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
    58981 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
    59041 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
    59101 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
    59161 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
    59221 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
    59281 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
    59341 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
    59401 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
    59461 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
    59521 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
    59581 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
    59641 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
    59701 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
    59761 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
    59821 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
    59881 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
    59941 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
    60001 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
    60061 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
    60121 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
    60181 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
    60241 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
    60301 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
    60361 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
    60421 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
    60481 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
    60541 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
    60601 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
    60661 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
    60721 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
    60781 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
    60841 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
    60901 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
    60961 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
    61021 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
    61081 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
    61141 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
    61201 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
    61261 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
    61321 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
    61381 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
    61441 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
    61501 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
    61561 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
    61621 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
    61681 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
    61741 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
    61801 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
    61861 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
    61921 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
    61981 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
    62041 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
    62101 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
    62161 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
    62221 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
    62281 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
    62341 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
    62401 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
    62461 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
    62521 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
    62581 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
    62641 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
    62701 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
    62761 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
    62821 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
    62881 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
    62941 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
    63001 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
    63061 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
    63121 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
    63181 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
    63241 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
    63301 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
    63361 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
    63421 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
    63481 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
    63541 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
    63601 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
    63661 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
    63721 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
    63781 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
    63841 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
    63901 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
    63961 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
    64021 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
    64081 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
    64141 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
    64201 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
    64261 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
    64321 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
    64381 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
    64441 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
    64501 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
    64561 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
    64621 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
    64681 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
    64741 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
    64801 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
    64861 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
    64921 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
    64981 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
    65041 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
    65101 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
    65161 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
    65221 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
    65281 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
    65341 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
    65401 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
    65461 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
    65521 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
    65581 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
    65641 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
    65701 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
    65761 ttctatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
    65821 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
    65881 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
    65941 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
    66001 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
    66061 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
    66121 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
    66181 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
    66241 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
    66301 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
    66361 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
    66421 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
    66481 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
    66541 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
    66601 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
    66661 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
    66721 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
    66781 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacggat atctcatgta
    66841 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
    66901 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
    66961 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
    67021 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
    67081 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    67141 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    67201 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    67261 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    67321 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    67381 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    67441 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    67501 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aatggcacaa caacacgtcg
    67561 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    67621 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    67681 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    67741 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    67801 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    67861 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    67921 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    67981 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    68041 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    68101 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    68161 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    68221 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    68281 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    68341 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    68401 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    68461 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    68521 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    68581 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    68641 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    68701 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    68761 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    68821 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    68881 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    68941 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    69001 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    69061 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    69121 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    69181 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    69241 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    69301 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    69361 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    69421 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    69481 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    69541 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    69601 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    69661 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    69721 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    69781 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    69841 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaactgcc gaaagcattt
    69901 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    69961 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    70021 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    70081 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    70141 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    70201 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    70261 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    70321 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    70381 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    70441 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    70501 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    70561 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    70621 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    70681 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    70741 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    70801 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    70861 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    70921 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    70981 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    71041 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    71101 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    71161 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    71221 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    71281 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    71341 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    71401 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    71461 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    71521 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    71581 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    71641 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    71701 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    71761 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    71821 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    71881 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    71941 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    72001 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    72061 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    72121 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    72181 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    72241 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    72301 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    72361 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    72421 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    72481 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    72541 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    72601 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    72661 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    72721 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    72781 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    72841 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    72901 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    72961 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    73021 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    73081 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    73141 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    73201 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    73261 ggtgaaaata ttgccattcg aggcttcaac gggattggga aatctacttt attaaaaaca
    73321 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    73381 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    73441 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    73501 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    73561 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    73621 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    73681 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    73741 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    73801 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    73861 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    73921 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    73981 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    74041 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    74101 caataaagta cataaacata aatacagtat tatttcggtt gcttgctgct tcacctagcc
    74161 cttgtactcg tgtttgattg gcgacttgac caaattgatt cccgacgtct aataaaataa
    74221 tagctaaaag caaaatggcc aaatggcttc cgccaaccaa taaagcaata aagcttattg
    74281 tttgcataac aagtcccata tacacgattt ttctttcaga ataagaatct gccagtcgtc
    74341 ccacgatagg tgcagcaact gctccagaca aaccaaaaat cgctaatatc cctgcttcga
    74401 atgttcccca attgtaggcg ggactgctga tataaaaaat caacgtagac caaaagatcg
    74461 aaaatgttcc aaacatgaaa aatccactga ctgccgcttc tcttaataga cgttgagaag
    74521 caactaaatg cggcaaactt ttaagtgaat caaggtatga aacagctgta ctactttgct
    74581 ggacagaacg cggcattttc cagtaaagca gaagtgtaag aattccgact gcacataaag
    74641 caaataaata gatcatccgc caagaaaaaa gactggcaac aataccagat accgtccgag
    74701 aaagcaaaat ccccacgagt aaaccgctaa gcaatgtacc catgaccttc cctcttgctt
    74761 ttggtcccgc tagtaccgca ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc
    74821 ctattaaaaa agaagcgcat gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca
    74881 gagagacggc agataacgct gccattcgaa tgatcaattt tggacgatcc accacgtccc
    74941 ctaaaggaac taaaaacagt aaccctaacg catagcctaa ttgggtcaac atcgtcaata
    75001 tcccgactgc acttgtgcta acagataaac tagctgctac ttttgttcca atcggctgga
    75061 tgtaatacat attggcaaca acaacgccac aagtgattga taatagtaaa ataagcgaag
    75121 ttgttaaagg ttttttttca ttactcttca ttgatttccc ccatttccta tcttagttta
    75181 caacttatgg aaaagattgg aaatagaaaa atacaactat tcgccacact tattatttgt
    75241 gcttttgttt tccttgtttt ttcttgcgat ctttataaat gaagacaact aaccctacta
    75301 gcacagtaac acttgcaacc aacgtgactg cagttttgtt atatccgtga aacagagctt
    75361 ctccaccaca cgcatacaac acagacgaag ggatgactcc aatcacgatc gacaatatga
    75421 tttgctgtag aggcaatttc atcgcatctg cagcaaaatt gaccatagaa gatggaataa
    75481 ccggtatcat atagcctaac attacaccta tttttggatg tttcatttgt cggattgctt
    75541 gcacccaatg attcgtttcg gttttcttat ctaaaaattt taagcgatgc ataaggaaaa
    75601 ttgcagtgat gttaccaaga gcattaccca aaatgttgat gatcgcacct gtcagtggtc
    75661 caaagctcaa accagcaatg actccaacta ctgaagtagg aatacctgga acagcacaca
    75721 tcaccgctgt caatacgatc aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg
    75781 cgagtagctc tgccttactc gctctcggat ttaaaaataa ttggatgtct gtctgatatt
    75841 ctaaatacaa ccgatagccc actaccagta tcagaatgat tcccaacgag atcaatatca
    75901 atcggattat tttctttttc atatttcctc ctgaaaaaaa tctatattca ttaaataata
    75961 gtttaacata aaaacaaaaa agcaccgaaa tccaacatag gatctcagtg cttttaaata
    76021 gtaaaaatta tttaacagtt acagatgcgc caacttcttc aagtttagct ttcaattctt
    76081 cagcttcttc tttagatacg ccttctttaa ctggtgcagg agcgccatca actacagctt
    76141 tagcttcttt caagcctaaa ccagttgctt cacgaactgc tttgataact ttaacttttt
    76201 ggtctccagc tgcagttaat tctacagtga attctgtttg ttcttcagca gcagcagcgc
    76261 caccagcagc agctacagct acaggagcag cagcagatac gccaaattct tcttcaatag
    76321 ctttaactag gtcgttaagt tctaggatag ttgcttcttt taactcagca acgatgtttt
    76381 caatgttcaa tgccattata atttcctccg tttaattgtt cttttttatt taatgcttta
    76441 tgcggcaatt aagctgcatc tccatcttct ttgtctgcca ctgctttgac agcgtaagcc
    76501 acgttgcgga ctggcgcttg tagtacagat agcaacatag atagaagtcc ttcgcgattt
    76561 ggcaattttg ccaatgctgt gatttcttcc aatgaagaga ctttaccttc gatgataccg
    76621 cctttgattt ctaatgcttt tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg
    76681 actacatctt cgttactgaa tgcaacagct gtaggtccag tgaatacttc gtccatgcct
    76741 tcaaggccag cttttttagc tgcacgtgaa agaatagaat ttttgataac tttcatttct
    76801 acgttagcat cacgaagttg tttacgtaaa cgtgttactt catcaactgt taatccgcgg
    76861 tagtcaacga ctactactga tgcagctgct gtaaattttt cgcttacttc atcaacgatt
    76921 gctgcttttt ttgcgataat tgcttcactc atttttgagt ttcacctcct gaattttgat
    76981 ggaagagttt tttgcactga aaaggcaagt cacttttata gttactttgc tcaaccagta
    77041 ttattcatca cctattcact tcgttcatag tgattctact cgctttgctc gaaaggaaac
    77101 tcctttcgct ttgctcaacc agtattgttc atcatctctt cacttcgttc atagtgattc
    77161 acaataaaaa actctatgcc accgcagaca tagagggact attgaatata ttcaataaac
    77221 gtcctcggta ggaaattaag gcttgatgcc acctactgtc ttcggtacaa gttaattatt
    77281 aacctcaact agcttagcat gctaaactag ttgagtcaag aattattttt cagttggatc
    77341 aatcaacatt ctaatattta attagaaaga agcttggtca acatggatac caggtccaaa
    77401 tgttgttgtg atcgttacat ttttcatgta ttgaccttta gctgctgatg gtttagcttt
    77461 tagcaataca tcatggattg ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac
    77521 ttttccgatt ggtacatgga tatttccagc tttgtccaca cggtaagtta ctttaccagc
    77581 tttcacttcg tttactgctt ttgttacgtc cattgtaact gtaccagttt tagggtttgg
    77641 cattaagcct tttggtccta atactcgtcc tagacgacct acagtagcca tcatatcagg
    77701 tgttgcaaca actacatcaa agtcaaacca tccgttttgg attttttgaa ccatgtcgtc
    77761 gtcacctacg aaatctgcac cagctgcttc agcttctttt gctttgtctc ctttagcaaa
    77821 aactaaaaca gtttgtgttt taccagttcc gtttggtaat acaacagcac cacggatttg
    77881 ttggtctgct tttttagggt ctacgttcaa acggtatgca acttcaactg ttgcatcaaa
    77941 ttttgcaatg tttgtttctt ttgctaaagc tactgcttcg gcaacaggat aaactttact
    78001 tgaatcaact ttttttaatg catcttgcat ttttttgctc tttttagcca ttttggttcc
    78061 tccttgattg tggttataac ggttgtacct cccacgtgtt ctatatcttt caatatagag
    78121 ccaactgaga agctgctgtc ttagggtgta tagattattc tacagtgatc cccatgcttc
    78181 gtgcagttcc ttcaaccatg cgcattgctg cttcaacgtt agctgcgttt aggtcttcca
    78241 tttttaattc agcgatttct cttacttgat cgctagaaac tttcgcaact ttatttttgt
    78301 ttggttcgcc tgaacctttt tcgattttag ctgctttttt caatagaaca gcagctggcg
    78361 gtgtttttgt aacgaatgtg aatgaacggt cttcgtatac agaaataaca accggaatga
    78421 tcaaacctgc ttgatcagct gtacgagcgt tgaattcttt tgtgaatccc atgatattga
    78481 tacccgcttg acctagtgca ggacctactg gtggagctgg agttgcttta cctgcaggaa
    78541 tttgcaattt aacgattttt tctactttct ttgccacgag acatacctcc ttaagtccgt
    78601 gatgtggtta attggagatg catatttctc ctcccacgtt ttatctgatc tgaaaaaatc
    78661 cagacaaata atgtgcaaaa caatgcacac tggtacagta tagcaaattt atttgttttt
    78721 tcaagatttt tttctgctat ttttacattt tgcttgaata aacgctaaat ttattctaaa
    78781 aaaacaacaa tttttcgttc tcatttcttc ttttatgtta cacttgcgct agcatggttc
    78841 aacagatgaa agcaaacaat aacacggttt tttagaccct tgtacaaaga ttcctacata
    78901 acaaaatgag gtggagaaaa tgaaagattt aaggtataga agtgtctttg atattattgg
    78961 acctgtgatg attggtccaa gtagctctca cactgccgga gctgctagaa tcgggaaaat
    79021 catacgtagt atatttggcg aacagcctga ttctgtagat atttatctat atgaatcatt
    79081 tgcaaaaacc tatcgggggc acggaacaga tattgcatta gttggcggcc tgttagggat
    79141 ggaacccgat gatgaacgtt tagctgattc tttgaaattg gcatatgaag ccggtatgga
    79201 agtcttgttt gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa agatgcttct
    79261 aaaaaaagga acacgaaagc tttcagtcac tggcatttcg atcggcggtg gcaatatcca
    79321 gatttctgaa ttgaatggat tcaaaatttc tttaagcatg ggtactccta ccttgatcgt
    79381 tgtccaccaa gatgttccag gtatgattgc taaagtgact agtatacttt ctgagaccaa
    79441 tacgaatatc ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat
    79501 aattgaagta gatgatcctc aagtagaaga aacggtaaaa agactcaaac agctgccaaa
    79561 tatcgacagc gtaaattatt tcgattaagg agctaaaaaa atgtttttat cgattgaaga
    79621 attagtagat caagcaaaag cttatccaag tgtagcagaa ttgatgattg cagttgaaat
    79681 ggaaatgagt ggccgttccc gtagtcacat catcgagatc atggaaaaaa acctctcagt
    79741 aatggaacaa tcaatcaacg agggaaaaga aggtgttacc tccgtcacag gaattaccgg
    79801 tggtgatgca aaaaaattag atgattattt aaatgaggca gactttttaa gtggagaaac
    79861 aatcttaaaa gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt
    79921 gatttgtgct acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt
    79981 ccgagatcgg cttgatttga cacatgaaca gcaattggaa ttcttattta ccgctggcgc
    80041 tttcgggtta gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg gatgtcaagc
    80101 agaagttggt tctgcaagtg caatggcagc agcagctttg gtttgtgcaa aaaatgggac
    80161 tcctcatcaa gcagcacaag cagtagccat tacattgaaa aatatgatgg gactgatttg
    80221 cgatcccgtt gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc
    80281 tcaagctttt atctctgctg atatggcatt agcgggtatc gaaagtgtca ttccgccgga
    80341 tgaagtcgtt gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac
    80401 agcagaaggt ggcttagctg ttacgccaac tgctaaacga cttgcggaag agattttaaa
    80461 taagagggcg tgataaagct ccttaatttc gagtattaag agattagtta tcttcataac
    80521 aaaaaacgaa gagaggacta attgcccttc tcttcgtttt ttattaatcc atcaatttcg
    80581 tattttttac ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc gacaattctt
    80641 tgttcaggtg ttcaaaaaca gatgttactc cttctgcccc acctagattc aacccataaa
    80701 tcactggtcg gccaatcgca actaagtcag caccacttgc cagagctttg aagacatgct
    80761 ctcctcgacg gacaccagag tcgaaaatca ctggtacacg tttgttgact acttcggcaa
    80821 tttttggcaa tacttcaaag gacgcaggac ctccatctag ttgacgtcca ccatgattgg
    80881 atacccagat accatctgcc cctgcagaga tagcgattac tgcatcttct ggcgattgta
    80941 ttccttttac gataactgga agatgggtga tttctttgat tgttttgatg tcatctggtg
    81001 tcaagccttg ttttgcagct gcataaattt ctgcgattcc ttttccttcg ccattaccac
    81061 ttgcactttg ttcgctatac gcagctaagt taggcattgg taatggaaat tggaattgat
    81121 tgatgacgtc ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa atgatagctt
    81181 tagcccctgc ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg tctttactca
    81241 tatataattg gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg
    81301 tatttgcata agtgctgatg gaaaagattg atccagcagc agctactcct ttagctgtat
    81361 cagcttcccc tttttcatgt gccaatcctt gggccgctga tggcgcttgg ataatcggtg
    81421 tatctaattc aattccgaaa acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata
    81481 tccgtggcat gattttcttt gtattaaaag aagtcgtatt ttctttcatc gtccactcat
    81541 cttcagaacc accgcgaata tagccaaaag ctcctgcttc catgcgctct tttacacgac
    81601 cttctagtta ggcaatgttt acaatatcga ttggatgttc ttcattactt gtatgataag
    81661 tcatagtaga tccttctttc atcttcttga ttacatataa atcatactaa acttacaaaa
    81721 agtaagcaaa catttactct cagatacttt tatcgctatt ctttccaaat aaatcgactt
    81781 acaagctgat ctacttcttg gttttcatga agcatactat gagaggcatt ttcacctgtc
    81841 actacttttt cctctatttc atttccgcgt tgcttcaaca acgcatagac cgctaatgca
    81901 ctgtttaacg gtactgttcc atcactcaag tctgtttcat ctagttgacc agcaatcaag
    81961 aaaaatcgag tggttatagg aatattaccg atcatttgct ggtaatcttg atagcggtta
    82021 gaaacgacag caggcccctt tgctaactca tctgtcagag actgcgcact atcatcgaca
    82081 aaatcattaa aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc
    82141 tgtccatacg ttcctaaata gcgtagtgca ctaactcctc ccatggaatg ccccactatg
    82201 ttgactttat ctatgccgta gttttctttc agatacaaaa gacaagtttt gatccattct
    82261 gcttgattcc actcgttatt tttattatct tcaaacaata cttggaccat cggattgtct
    82321 gcttttccag acaaatctcc tgttgcttgt atttgtccat ctgttcctac tgttaaaacc
    82381 aattctttta ctgcttgctg atttttttcc aaacgccgca acatcccttt aaaggaattt
    82441 tctgtcccgc tgtagccgtg gaaaaaaagt gttggaatag tggttttttc tatcacttta
    82501 ctggattctg ccgttttttc tttatcagaa acttcagtag tatttatctt cgtcgtctgg
    82561 caaccactta acgcaaacaa actgataagg actaaagctg ctgcagttgc acgtttgcat
    82621 aaatttttga ttggttctgt catacacctc ttcctttcca tatagaaaaa agaaagactt
    82681 gaaaggccaa tcatatgatc gacatttcaa gtctctttat taatcgattt tatctacttg
    82741 atcgaaatca agttctgtac tggtttcacg tccaaacata tcgatgttga ctttcaattt
    82801 ttctttttct ccgtcaactt ctgttacttg tccttctaat ccagcaaatg cgccttcgat
    82861 gattttcacg acttcgccaa cttctacttc tagatcattt tgtcgcaggc tcataccgat
    82921 agaacgcaag atatgattga cttcttctgg aagtaatggt gctggtttgc ttccggctcc
    82981 gtgtgaacca acgaatcctg tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc
    83041 tgtcatgatc atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga tttctttttc
    83101 tttcccattt ttgacttcag tttctttttc ttcaggaaca acgacacgga aaataaagtc
    83161 ggccattttc atactttgcg cacgagattc gatatttgct tttactttgt tttcatatcc
    83221 tgaatatgta tgcaagacat accagttttt ttcaaaagat tccattgaag gctcctcatt
    83281 tcattgtaaa ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt cattatagca
    83341 ctaaaagtac gttgtggcta gcgagttatt ttaaaatcca gccaaataag ctttggatgg
    83401 ctgtatccat tacgtaaaac atcaatccga aaataataga agtttcgatg actactaacg
    83461 tatctttacg gagttgtttt ctgcttggcc aagttacttg tttgatttca tcttttacgc
    83521 tttttagaaa tttcatgaga caacctccct tgctactgtt tatttcgttt ctttatgtaa
    83581 tgtatattga ttacaatatt tacaaaactt attgatttcc aaacgttcgc cgctttttcc
    83641 ttcactgact gattttgtgt agttgcgaga cccacaaacg gaacaagcga gtgctgcttt
    83701 tttctttgcc atttacttct gcccccttgc tgagaatcag acttatactt cataacagta
    83761 tcacgtaact ttttacttgt caatttgtga aataaactat ctattgtttt ggataaaatt
    83821 atgatatgat taatttgaaa ttaagctaaa atgagacaat tggaggtata gcgatgctat
    83881 taaaaacact tgtatataag aaaaaagatc tgacaaccgt tacagagact agtacattag
    83941 aagaagcatt aaaaatttta gaaacttccg gataccgttg tgtgccgatc ttagattctt
    84001 caggtaatat tttccgtgga aacatttaca aaatgcatat ctatcgtcat aaagcaaatg
    84061 gcggtgatat gacgcttcct gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc
    84121 tggatacttc tttcttcaaa gtattcttta cgatcaaaga gcttccttat attgctgtcc
    84181 ttgacagtga aaatcatttt tatggtattt tgacacatag tacgttgctt aatatgctct
    84241 ctcaatcttg gagcgtagaa caaggaagct atgtactgac aattgcttcc acaggaaagc
    84301 aaggagattt ggctgctatt gcaaaaatca tttcaaaaca cagcagcatt gccagctgta
    84361 ttactcttga tatcgaaaaa gacgaataca ttcgacgtac attgatcacg ctaccggcta
    84421 acactgaagc gaaagtatgt gaaacgatcg tcaaccactt agagaaaaag aattataaag
    84481 tggtagaaat tgaaaatttg caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac
    84541 agctaatctg ctttcttttt tcgcttttta taattagaca cgcttttcaa aatcaagcgc
    84601 gcgatctaaa taatacatca atggtgctga aacagccatg acgataaact cacttaatgc
    84661 aagcgtgcca taggttgtcc aaaatgcttg gccacctgtt ggaactaaca tataagcaat
    84721 cagtcccatg ctgatggtaa agaagatcgt gttcaatact aaacgcatct ttacattttt
    84781 cactttgttt tgcaataaag ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa
    84841 aaccacatcc atgataccaa aaccaaagaa ggcattatag acgatgaccc cgcctaaaac
    84901 tccccaaagc aatttacggt taaagacaac taaatgattc aaactttctg aaatacgaaa
    84961 ctggatcggc cctgaggata caggcgcaac taaaatagtt aacgctagat aaagagccat
    85021 gatgatgcca tttacaacga ttacggataa ccgttgtctg cttttcgttt tttgcatttt
    85081 ctctcctctt ttctcctagt ttttttaacg cgggatggtt gatgaaccgc ggttatgtga
    85141 aacaaacaat aattataaca gacttttcat ttatttcaat ccgtcttatc tgcttttaaa
    85201 aaagcaaaag cttaaacaat gtaaacgttt gctaaaaatc gtgggatatg gtataaagta
    85261 agtgtaaggg gtcataagaa ttcccagtaa agcagacgaa gtaggatctg caagaacaga
    85321 attgttgtga aacttaaata aataaaaaag gatgtgggaa tttgagcaac ctcttactag
    85381 agaacggtct agcaagcagc tgaagatcta agtggacatg ccgaaccttg gaacttctcc
    85441 ttacgtaagt aatggatcat gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc
    85501 tttttcattt tcctagactt ctgctataat aaccaagaat taattttgta acagattttt
    85561 ttgaaaaaac aagtttcttt cattcaaaaa atagaaaaag cttgctaatt attggttaac
    85621 cagcaaacag gagtattaaa atatcttgtt ttattgaata gcaggaggaa atcatgaaat
    85681 tccattatca tatattgatc aatccatcgg caggcagcgg aaatggctat aaagtagcag
    85741 aaagaatttt accggtatta aaaaataagc atattgacta tacacttcat tatagtgaat
    85801 acaaaggaca cgatgctgag attgctgaaa ctttggcaaa agaaacattg attccttgga
    85861 tagaagaaga acagaaaaca atcgatactt tccccctttt gatcatcgta ggaggtgacg
    85921 gcaccttaca tcaggtactt gatactttct atcagatgga agtagaattc ccagttgctt
    85981 atattccagc aggttctgga gatgattttg ctcgaggggc aggccttcca aaaaatccca
    86041 aaaaaggact tcagctgatt ttagcagctc aatcaccaga aaaagtccac ataatggctt
    86101 atgaagaaaa aataagtgaa aaaaaaggaa tagctgttaa taatttcggt ataggattag
    86161 atgcagctat cgtccatgcg acaaatcatt cttccacaaa aaagcgattg aacaaataca
    86221 acttaggatc attttcgtat ttattctcta ttctccgtgc tttattcact caaaaaggat
    86281 ttccaattct ggtagatgca ggaggaaaac aattaagttt ttcaaacgca tttttatgta
    86341 cagcaacgaa gcatcctttt tttggcggag gaatcgcgat tgttccaact gctgatgcgt
    86401 ctaaaccagt cattgatttt gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc
    86461 tcattctttt acttatacag aaaaaacaat tgaaatcgaa atatttccat cactacacaa
    86521 caaacagatt acgtatcgtt tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa
    86581 tggaaaaacg accttttgat atcacaataa ccacaactaa acagttgttt tggtgttaat
    86641 aaaagaggtt ttgtcagaag ctacatctgg aacaccgttt attcggaaaa aaggactgtg
    86701 acacgacttt tgtcacagtc ctttttttac tatgcagtta attctttgcc caattgggtt
    86761 gccatcgata gtttctttct ttacccaaaa taaattggat cattgaaaac accgcagcaa
    86821 aagcaactaa gacacaacca ttgaaccaaa aagattctgc aaatgtttga ttgtaactga
    86881 gtgtttcact gatcaagtta ttattttgaa caaaccaata tgttggtgtg aatgccgcta
    86941 ttttgctaac catttctggc aagtattctg ctggtacaaa caccccaccg ataaagcaac
    87001 tgcctgtgat aaaaatatct ttgatcccac cgctggtttc tgagtttttt accaatgaag
    87061 tgatacaagc cgaaaagcta atgatcggca ataaaaacaa gaaagtattt aaaatatgat
    87121 accataccgt tatatcccac gaggtgtgga tcactaacag aaccattaga agtaagaagc
    87181 cactgaacaa cagcagcgag taactaatca aactaaaggt tactttgcga ttcaagtttc
    87241 gtttggtaat gggcgcactc tggtttcgtt tactgatctc tttgcgatta aatgctagat
    87301 tgatgacgct gaaaccgcta aaaatggata gaaacaaccc ataagacaag aaattaaagg
    87361 ttgtgcccgt caacagcttt ttcatccgct gactatatcc cgcacttagc tcaacttctc
    87421 cagtaaccga gagtgccatt tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt
    87481 cttgaaacag aatcagtgtt tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag
    87541 aaaaggcatc tgggcgactc tgtatcttga tcgtcggcat ctttcctact tcgacttgtt
    87601 cggtaaagtc tgcaggcaga tgtaagataa tgtttacacg attaaaataa agagcatcat
    87661 cgatttcttt ttgtgaggtt ccatccaatg taacgactgt ttccttgtca ttcaaatatt
    87721 tacgaaggcc atcgactaca ggcgactctt cttcactaat caaggcgatt ttcgcatctt
    87781 gcagagaggt attttcaaca tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga
    87841 taattacacc tatcagtaaa ttcgatttat ttgctttaag caatgtccaa atcgttttat
    87901 aaagcgtcat aacgcacccc tctttccatc caagcatttc ctaacgcaaa aaggaaaata
    87961 aatccgatca accatagaag attggtataa aaactagcca aatcagtgta gaaataaagt
    88021 ttatacaaac tttcgctgat caagttgacg agatttaatt gaccaacaat cggaatatga
    88081 agatcaatcc aatacttcac ttgttccgac cccatcatac cagcaaaaaa gctcatcgca
    88141 atcgtcactg aaatcaagat gctatccttt tgcaccagat tcatttttgg tatcaaatta
    88201 ccaaccaatg tacctaacaa aatcgcacac aacgacccca gagcggtaac aatcatcaac
    88261 cattgccaac gttcaccaaa gtctacttga tagataaaac gaaagacagc taacataata
    88321 taaatctctg tcaaaacaat cgtaaagctt gctgccaaat tagcgattga aactaacgat
    88381 tgctttctag gaatcaagga caagcgaata ccatttgacg attgatttgc ttgctgatcc
    88441 tgtacattgc ggacccccca catcgtcccg tagagaatcg ccattccaat cagagtgaag
    88501 aaataaaaac ttttgaaact catattacga cctgcatgaa cttcttgaaa agatacttct
    88561 tgttcaaaaa cagtcatgat gggcgcgaga tcaacgttct ccacttctgc cactttactc
    88621 gaaattatac caatacgctg taaaaattga tccatgacgt ttttcatcaa cgtctgactc
    88681 attcctgctt gacccacatg gagcgaaaca gatgaaccat caatgagata aaaaccagcc
    88741 aaatcatctg catttagctt ttcattcgct tctacttccg atagttcctg tacttcaaat
    88801 aatggtttat cattgacttc gacttgttct aagactgtgg taaaattttc cgaaagcgct
    88861 gtttgatccg ctttgattac aattccagta ttaatcgttt cgagattgcc accttgatca
    88921 atctcgccaa aggccgccat aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa
    88981 aacaacatgg atttatttcg cagcagtatt tttgtgcgat atttataaaa atgccaaaac
    89041 attatttccc ctcctctagt ctctcaaatc cttgccagta atctctaaaa agacatcatt
    89101 caaggacgct tctttggttg ctatttttcc gtaactgatc ttttcttctt gtaagattgt
    89161 aagaatcggc aacaatactg tatttagttc agtagttgtt acttggagct gacctttttt
    89221 ataagcgatt ccatcagaag ctattgttcg ctgacaacac gttttctggg agtaatggga
    89281 tgtcgatcgt caaacgatcc gccgtccgta ccatatcttt tagagattct ttggttcctt
    89341 tagcaatcac ctgtccttga tccatgatca cgatctggtc gcaaagaatt tctacttctt
    89401 ccatgtaatg tgttgtatag acaatggttg ctccttgtcg attcagctcc ttgatacttt
    89461 ctaaaatttt attccgactt tgggggtcta cagctactgt cggctcatct aaaaaaatta
    89521 accgcggctt gtgggcaatc ccacatgcga tgttcaaccg acgtttcaag ccaccactaa
    89581 gttctcctgg acgaaacgaa acatattttt ctaatcccac taattgaatc acctcttcaa
    89641 tcaaggcttt ccttttctct ttttcggaaa catataaacc gcaaaaataa tcgatatttt
    89701 cttgcacact tagttcgtca aagaccgcaa cgtcttgagg aacgacccca atcattgcct
    89761 tgacagcata attttcagga gtcattgttg tcccgaacaa tttaatggtt cctttgtcat
    89821 aatttaacaa agaaagcata cagttgatgg cagtcgattt cccactgccg tttggtccta
    89881 acagacctaa gacttctcct tcttgtactt gcagatcaaa atgattcaat gctacaaaat
    89941 ctccatagcg tttgactaat ttatcaattt cgataatcat catgaaaaac tccttccaaa
    90001 tttcttgttg atctgactat aataaagtgc aagaaaagaa actagtgacg attggctaaa
    90061 ccacacatga catttgtcac aatgaaggag tctgctatgc ataaatctcg gctactcaac
    90121 gaaactagct ttctactggt tatctcattg cttttgctgg taagtcaagg attcgttgct
    90181 atgcaagtta tctatttatt gatcgccgtt ttattcactg gtgtcagtta tttactgcct
    90241 acaaagccaa attggatttt ccaagttatt ctatttcttt tatgtgcctt tttacctgat
    90301 cttgtcctgt ttctgccagc gacgatgcga gtgatcgccc ctgaagaaag ccaccttgat
    90361 tacttgtttt tgttaggttt tttgttgatt tcgacggttg gtaatcatcc ccccatcaag
    90421 gtcgcctttg tcgtaatttt gctgtttttt tccttttact tacgaaaaaa agatcaacga
    90481 acgaccgatc ttgagaaaaa gtacattgtc tccaaagata ctcattggga aaaacaacaa
    90541 gagttatcgg aacaaaataa agcattggcc ctgtcacaag aaacacaatt agccttgcaa
    90601 attgtggaag aacggaaccg aattgcccga gacatccatg acaatgtcgg tcatctgctg
    90661 tcaagtgcga ttttacaaat tggtgcgctt gaaatgatca atcaagaacc aaaactaaaa
    90721 gaaccattag aaaatttaag tgccaccgtt cacactggga tggatcggat tcgtcaaagt
    90781 gtccatgacc tccatgagac atcgatctct tttcaacact ccttagagtt tctgatcgct
    90841 gattttcctt cttctgttac gattgaagga aacttgtttg aaggattgaa tgaaacgcag
    90901 caaaactgct tcttaatggt catcaaagaa gccttaacca acagtatcaa acatagtcat
    90961 gccaaaaccg ttgttttaca ttttcgaacg ttgccggcct tttatcgctt gcaaatcacg
    91021 aatgatggca ccagcccaca aaaaaattct gctcccggta ttggtttgaa aacgatgcgt
    91081 caacgtgttc aagaacaaaa gggacaagtc catattttcc aagaaaacca gcaatttcag
    91141 ttaacgatca ttttaccaag ggagggacac caatgataac agtagtcatt attgagatga
    91201 tccatttgtg acgaaatcgt tacaaaccat tttagaaagc acgaaagaga tccgtgtgtt
    91261 aggcattggt cattgtgcca aggatgcgct agtcttatac gaaacccatc gtcccgatgt
    91321 tttgctgacg gacatccgca tgcctaaaca aacgggcatc gatgctgcca aagaaatttt
    91381 ggctaaattt cccaaagcca tccttttgct tttgacgaca ttcaaagatg aagaatacat
    91441 ccgcgaagca ttttccatcg gtgtcaaagg gtatttaatc aaacagaact tgcaagcgat
    91501 tattccttct gtcaaatccg cttataatgg acaggccgta tttggtaatg aaatcatcga
    91561 gactttcact caaatgatga agaataaacc gacgattgat agtcatgctt atgcttcttt
    91621 tagtgaacgt gagcttgcaa tcatcaaaga agtagctgcc gggaaaaaca ataaagaaat
    91681 tgcagatgcc ttgtacttga gtgacggcac tgttcgcaat tacatcagcc aattactaga
    91741 aaagctagat ttacgggatc gcacgcaatt ggctatctat ttttatcagc atttgcaata
    91801 agtcttatct aacacaaaaa aacctgaatg atgctccttc gcatcattca ggtttttttc
    91861 acatcttcta ttcaattgtc ccgcaaacca tttcctcacg attgaaagaa tcaaggattc
    91921 gcagggctgc caggattcgt tggattgttc ggcgtgttag gcggaaaaat aggcgccttg
    91981 tctaacgttt tttctagttt tgattcttca cgatcgtctc gttctacatc aggactttca
    92041 ttcaatttga attctttttc catctcatct aactttttaa agtcattgcc agattctttt
    92101 gctaatttcg ctaatttatc ttcggtttct ttagccataa agtaacgccc cccttttgat
    92161 ttcagcttag catatcacac tgaaaaaaca aaagaaaacg ctaaccaacg atcattttta
    92221 gctatttctc atctttaaaa tcaagcattt tttgccggta taaaatcgta gggagaactg
    92281 ttttccatac caatcatcgg atctactaac tggttatcga tcacttctgc tgtaagaacg
    92341 aaatctttag aaacagcagg ttctacctct tctttctttt cttctccaac aaatgaagaa
    92401 aacagatctg gttccactgt ttccattact tggtttttcg tttcattcgt cccactgtca
    92461 tcttctacag gtagcttttc ttttacaacc cgtggctctt ttgtatagtt cccatcttca
    92521 aatggatcat ttccgaccaa agaatcttca taagctgcta gtttggtccg gagagtgata
    92581 ttcattttat cattatccat gatccttgat ttcaacgtag tcatccggct tgctgattca
    92641 ttcgctacac aagtgacata ggcttgctct tcccctaaca aaatcaacag ttccttactg
    92701 cgagtcactg ccgtgtagag caaattacgc tgaagcatcc gctgatattg atggaccatg
    92761 ggcaaaataa ccatcttgaa ttcactgcct tgtgatttat ggattgagca acaataagaa
    92821 agagtgattt tattccattc gtttctttta taagtgacct catttgcatc aaactggata
    92881 actaattcat ccactttatc ttcagaatct tttgccaaga tgatacccgt gatttctccc
    92941 atatctccat tgaatacatt caattcggga gtattgacta attggaggac tttgtctcct
    93001 atccgataaa cggtgtcgtt ccaatgtact tccttttttc caggtttccc tggattgaat
    93061 atctcctgca tcattttatt taacgcatcg atccctgcag gtccccgata cattggtgct
    93121 aatacttgga tatcttgtgc acggaatccc ttatttttcg ctttttccac aattttagag
    93181 atcaagggtt caatttgata agcatcactt cggaaatagg aacgatcttt ttgattcttt
    93241 gtgaaatctt ttggcagacg tccttctttg atctcatggg caagaggaat aatactagaa
    93301 ccatcacctt gccgataaat ctgattgagt tccattttgg gaatttgttc gatttccaaa
    93361 agatcatgga gtacttgacc aggaccgaca gagggaagct gatctttgtc tcctactaag
    93421 ataacttgca tattcgtcgg aatcgctttg aataatgtgt tagccagcca tgtatcgacc
    93481 atagacatct catcaacaat caaaagtccg ccttctaatt ctttcgtact gactgaaggt
    93541 gttttttccc gtccattcag ccccagaaga cgatggatcg tactggcagg cagacctgtc
    93601 gtttcgttca ttcgtttagc tgctcggcca gttggtgctg caagtagaat aggaaaaatt
    93661 tcttgggtat attttgaagg atccaattcc agtccgttca actctgcaaa aagattgacg
    93721 attccgttga tcacagtagt tttccccgta cctggtcctc ctgttaaaat aaatagaggg
    93781 gaacggattg cttcagcgat agcttgttct tgcgaaggtc catactgtat gccgaatatt
    93841 ttttcgatac gccgaatatt cttctgcact tctttttcag gataatggat ttctttttta
    93901 cgactgagaa ggcgctggat cgaattaccg atcccccatt cagaaaagta caaactgttt
    93961 tcgtaaagat tagtctcttc ttgttgtatc tttccttctt caacaagttg gataattaca
    94021 gtagcaacct gatcaggctg gatttcaatt gggcgacttg tttctaaaat acggatcgtt
    94081 tgctgaagca atgtctccgc cggtacgtat gtatctcctg tttgaactgc atgctgaaaa
    94141 atctgatgca agatagctgc tcgaattctt ctcggtgaat ctgctgcaat accgatttgt
    94201 tcggctagat tatctgcacg tttgaacccg attccttcta tgtcttcgac tagctggtac
    94261 ggattttctt cgatgatatc caaagactcg ttcttatagg cctggtagat actgaaagct
    94321 aactgactac cgaacccgta acgattcagt cccatgatca cttgatccat cccgtggttt
    94381 agccgaatcg tctcagcaag catttctctt tttggcttcg tcaaaccgga gatttgtttt
    94441 aataccgaag gatcagcaat gattttttcg atagcatctt ctcccagcaa ttctacaatt
    94501 ttttctgctg ttttcttccc gattcctggg aatttttcgc tggaaagata cgctacgatc
    94561 ccatttgcag ttgttggctt ttcttgctca tatctttcga gtttaagctg ccttccgtat
    94621 ttaggatgat ccactaattc tccataaaaa cgataagtct cattttcttg aatatcgccg
    94681 aaacttccgg tgactacaat ctctttttca cggtaatcag ctgtggtttc actgatgtgt
    94741 accaaaagca ctttgtaaaa attactagga ttttgaaaaa aaatcgcggc aactgtacca
    94801 acgaaataag gcatttcttc tggctgcatc ggaagccgcc tccttcttct atagtaaagg
    94861 ttctggtgct gtttcagttc ctaaaaaatc tgtttggttc catactttca gttcatatgg
    94921 caataatctg caaggctctt cagtctctaa aatcgtcaaa ctactattga acaatccacc
    94981 catttcacgc aactcactta gctcttttcc tgtcatccat tgaatggctg cagttaaaga
    95041 agctccatgt cctacgaata aaagtgggcc atctttatgt tgggcaaccg catctgctac
    95101 aacagtttcg atacgtgtga ttgccgattc aatcgtttct ccgtgaaaag gagaaggatc
    95161 atacagatct aaatgattac gcaaattcgg cagattatcc ggatatttct ggtacatttc
    95221 atcgatcgac tgaccttcta aagtacccag ccctagttct cttagcttat ctgtataaaa
    95281 aatctctacc ggacgagcaa gctctttgtt gataccttct gctgtatcac gtgctcgctt
    95341 tgctgtactg gaataaattg ctttaaaagg aacatatttt acctttttcc caaatgcttt
    95401 gatttcttcg taactttgtg gcaaaagcgg agaatcgcca ttgctccctt gaaaacgtct
    95461 ttcctgattc cattgtgtct tcccatgtcg ggtaaaatat agttccatac gcagcaaact
    95521 cctgtctttc ttttatttta ctctataata aactgtctaa tccagcttgt cattttcata
    95581 gtgatgcgtc aattctaaat tagggaatcc ttgctggcga agtgcttcat acactatcat
    95641 tgcagctgta ttcgataaat tcaatgagcg gacatgctca tcattcatag ggatgcgtaa
    95701 acacttttct tcgttttcac gcataaatgt ttctggcaaa ccggttgttt cttttccaaa
    95761 taaaaagaag tgctcttctc catcagcaaa attttcgtca ctatatactt tatgggcaaa
    95821 ttttgagatc aaatgtagtt tacgattacc taaaaatgtt aaaaaagctt ctaaattact
    95881 gtggtaagta atatttacat cattccaata atccaaccct gcccgtttta gatgtttgtc
    95941 atcagttgaa aaacctagtg gctcgattaa atgaagcgga gtattcgtgg ctgcacacgt
    96001 acgggcaata tttccagtgt ttgcagggat taatggttca aataaaacga tatgattcat
    96061 caggatcatt cctctcatat ttatgttcgt ttctattttc tgacaatttg atttttgcat
    96121 aaaaaaaaac gtatcaactc ggtgtcagtc actgcgagtc gttacgttag tgaagctctt
    96181 ctcactaact tttatcagaa caggaacctg ataaaaacca atgaaaaaac aacttgttgc
    96241 taatataaca ccctcaactt ttttttgcaa cctctactca ttaaaaactg ctagaaaaac
    96301 gttttctctt agttcctttt aataattcta tgtcaattgt aatcttcttc aaaggattct
    96361 tatataagtc tgctttgatt cgctcttcta cctgccattc taaaggtgac atggccaaaa
    96421 gatctttttg attttcctct ggtatctcaa attcataggt tacacgctga cgttcagtta
    96481 atttcattgc ttcagcaaac ttggctacta cacgttcatt ggaataactc tgtttttcag
    96541 gttgattcgg ataaaaagcc tgacgtagtt cctttaaata attttcttgt ggcaccacct
    96601 tcaacacttc tccatcttct gagagtacac gttggaattc ttggtaatga gaaggcgaaa
    96661 agatatttaa aaccgcgttg aatgtttgat cagcaaacgg caaattggtc aaatcagcta
    96721 cacaccaaaa tgcttcggca gattgttctg tagctgcgta gactccttct tttgaaatat
    96781 caaatccaac agcattttga atctttttca attttgaaag ttcattcaag aagctacctt
    96841 ctccgcagcc aacatctaat agcgagactt cttcaggtaa atgctgcgca atagtctcaa
    96901 taacgggctg atacatgcca gattggatca ttctacgcct tggaacgaac attccttgat
    96961 cgtattctgt tttaaccgca tgggataaaa aataaagtgt tccttttttg gaaaggtcaa
    97021 aacggtgatt atttttgcat atcaagccat tatcagactt ttttacttct tcttggcaaa
    97081 ccggacagcg gaacagctga tgattttctg ccaaaaactg gcgtcctcgg tcaatttttt
    97141 ttaacatagt tccccctcat ttctgtttta tcttaccgtt agatgctcac tttggcaact
    97201 gctaaaaata tgatacaatg cctagaagaa agtgaaagat aaaggagaag attagatgat
    97261 caaagaattt tgcgctgaaa actacacatc tattcctcta gcaattgcta gaggagccga
    97321 tcggatcgaa ttgtgtgaca acttagcggt cggtggcaca accccgagta ctggtgtgat
    97381 cgaagaaacg ttaagttatg ctggggaaaa agatatcccc gtaatgacga ttattcgtcc
    97441 tagagggggc gacttcgtct ataacgatat cgaattgaaa atcatgcata ctgatttgat
    97501 tgaagcaaag aaattaggga cggatggcgt agtatttggt tgtcttactc cttcaggctg
    97561 gctagacgaa gaggccttag aactattgat cgaaacagca gaagggttgc agatcacctt
    97621 ccatatggct tttgacagta ttcctgaaga tcggcaattc gaagcaatcg attggctggt
    97681 agaacatggt gttgaccgta tcttgactca tggcggtcct gctgacaaaa ctattgtaga
    97741 aaatttccca cggcttaaag aattgattgc ctatacagat ggccgcttaa tcattttgcc
    97801 tggtggtggt gtcactgcag agaatgcagc acatgtagct aaagaattga acgtaagcga
    97861 agtacacgga accaaaatcg tggatcttca gttataatct tataaacaaa aaaacatttg
    97921 caacaggcat aatgcttatt gcaaatgttt ttgttttttt acataaaata gcccataaat
    97981 aaagaagcaa tattgaaata aatc
//
LOCUS       DACEDO010000003        90181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEDO010000003 DACEDO010000000
VERSION     DACEDO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.003"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            315..3149
                     /locus_tag="IWO52_000193"
     CDS             315..3149
                     /locus_tag="IWO52_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0354491.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3341..3832
                     /locus_tag="IWO52_000194"
     CDS             3341..3832
                     /locus_tag="IWO52_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0354492.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3935..4924
                     /gene="manX"
                     /locus_tag="IWO52_000195"
     CDS             3935..4924
                     /gene="manX"
                     /locus_tag="IWO52_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0354493.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4957..5760
                     /locus_tag="IWO52_000196"
     CDS             4957..5760
                     /locus_tag="IWO52_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0354494.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5781..6695
                     /locus_tag="IWO52_000197"
     CDS             5781..6695
                     /locus_tag="IWO52_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0354495.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6778..7152
                     /locus_tag="IWO52_000198"
     CDS             6778..7152
                     /locus_tag="IWO52_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0354496.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7291..7536
                     /locus_tag="IWO52_000199"
     CDS             7291..7536
                     /locus_tag="IWO52_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0354497.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7708..8772
                     /locus_tag="IWO52_000200"
     CDS             7708..8772
                     /locus_tag="IWO52_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0354498.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9018..9443
                     /locus_tag="IWO52_000201"
     CDS             9018..9443
                     /locus_tag="IWO52_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0354499.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9436..9885
                     /gene="nusB"
                     /locus_tag="IWO52_000202"
     CDS             9436..9885
                     /gene="nusB"
                     /locus_tag="IWO52_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0354500.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9982..10836
                     /locus_tag="IWO52_000203"
     CDS             9982..10836
                     /locus_tag="IWO52_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0354501.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10837..12174
                     /locus_tag="IWO52_000204"
     CDS             10837..12174
                     /locus_tag="IWO52_000204"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0354502.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12179..12403
                     /locus_tag="IWO52_000205"
     CDS             12179..12403
                     /locus_tag="IWO52_000205"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0354503.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12405..13295
                     /locus_tag="IWO52_000206"
     CDS             12405..13295
                     /locus_tag="IWO52_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0354504.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13304..14122
                     /locus_tag="IWO52_000207"
     CDS             13304..14122
                     /locus_tag="IWO52_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0354505.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14229..14678
                     /locus_tag="IWO52_000208"
     CDS             14229..14678
                     /locus_tag="IWO52_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0354506.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14696..16378
                     /gene="recN"
                     /locus_tag="IWO52_000209"
     CDS             14696..16378
                     /gene="recN"
                     /locus_tag="IWO52_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0354507.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16433..16549)
                     /locus_tag="IWO52_000210"
     CDS             complement(16433..16549)
                     /locus_tag="IWO52_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0354508.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16622..17578)
                     /locus_tag="IWO52_000211"
     CDS             complement(16622..17578)
                     /locus_tag="IWO52_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0354509.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17749..18117
                     /locus_tag="IWO52_000212"
     CDS             17749..18117
                     /locus_tag="IWO52_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0354510.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18287..18718
                     /gene="mraZ"
                     /locus_tag="IWO52_000213"
     CDS             18287..18718
                     /gene="mraZ"
                     /locus_tag="IWO52_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0354511.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18739..19698
                     /gene="rsmH"
                     /locus_tag="IWO52_000214"
     CDS             18739..19698
                     /gene="rsmH"
                     /locus_tag="IWO52_000214"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0354512.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19703..20104
                     /gene="ftsL"
                     /locus_tag="IWO52_000215"
     CDS             19703..20104
                     /gene="ftsL"
                     /locus_tag="IWO52_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0354513.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20105..22291
                     /locus_tag="IWO52_000216"
     CDS             20105..22291
                     /locus_tag="IWO52_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0354514.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPSED
                     PDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNL
                     EADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGW
                     SKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22317..23279
                     /locus_tag="IWO52_000217"
     CDS             22317..23279
                     /locus_tag="IWO52_000217"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0354515.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23293..24654
                     /locus_tag="IWO52_000218"
     CDS             23293..24654
                     /locus_tag="IWO52_000218"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0354516.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVSECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24680..25768
                     /gene="murG"
                     /locus_tag="IWO52_000219"
     CDS             24680..25768
                     /gene="murG"
                     /locus_tag="IWO52_000219"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0354517.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25783..27003
                     /locus_tag="IWO52_000220"
     CDS             25783..27003
                     /locus_tag="IWO52_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0354518.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27159..28484
                     /gene="ftsA"
                     /locus_tag="IWO52_000221"
     CDS             27159..28484
                     /gene="ftsA"
                     /locus_tag="IWO52_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0354519.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28507..29748
                     /gene="ftsZ"
                     /locus_tag="IWO52_000222"
     CDS             28507..29748
                     /gene="ftsZ"
                     /locus_tag="IWO52_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0354520.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29765..30442
                     /locus_tag="IWO52_000223"
     CDS             29765..30442
                     /locus_tag="IWO52_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0354521.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30456..31055
                     /locus_tag="IWO52_000224"
     CDS             30456..31055
                     /locus_tag="IWO52_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0354522.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31070..31357
                     /locus_tag="IWO52_000225"
     CDS             31070..31357
                     /locus_tag="IWO52_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0354523.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31406..32188
                     /locus_tag="IWO52_000226"
     CDS             31406..32188
                     /locus_tag="IWO52_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0354524.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32209..32916
                     /locus_tag="IWO52_000227"
     CDS             32209..32916
                     /locus_tag="IWO52_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0354525.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33178..35976
                     /gene="ileS"
                     /locus_tag="IWO52_000228"
     CDS             33178..35976
                     /gene="ileS"
                     /locus_tag="IWO52_000228"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0354526.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36025..37551)
                     /locus_tag="IWO52_000229"
     CDS             complement(36025..37551)
                     /locus_tag="IWO52_000229"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0354527.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37566..38213)
                     /locus_tag="IWO52_000230"
     CDS             complement(37566..38213)
                     /locus_tag="IWO52_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0354528.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38397..38726)
                     /locus_tag="IWO52_000231"
     CDS             complement(38397..38726)
                     /locus_tag="IWO52_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0354529.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38903..39631
                     /gene="sfsA"
                     /locus_tag="IWO52_000232"
     CDS             38903..39631
                     /gene="sfsA"
                     /locus_tag="IWO52_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0354530.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39647..40660
                     /locus_tag="IWO52_000233"
     CDS             39647..40660
                     /locus_tag="IWO52_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0354531.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40660..41937
                     /locus_tag="IWO52_000234"
     CDS             40660..41937
                     /locus_tag="IWO52_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0354532.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42000..44702
                     /locus_tag="IWO52_000235"
     CDS             42000..44702
                     /locus_tag="IWO52_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0354533.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44854..45171
                     /locus_tag="IWO52_000236"
     CDS             44854..45171
                     /locus_tag="IWO52_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0354534.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45201..45521
                     /locus_tag="IWO52_000237"
     CDS             45201..45521
                     /locus_tag="IWO52_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0354535.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45629..47089
                     /locus_tag="IWO52_000238"
     CDS             45629..47089
                     /locus_tag="IWO52_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0354536.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47157..47378
                     /locus_tag="IWO52_000239"
     CDS             47157..47378
                     /locus_tag="IWO52_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354537.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47409..47591
                     /locus_tag="IWO52_000240"
     CDS             47409..47591
                     /locus_tag="IWO52_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0354538.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47591..48004
                     /locus_tag="IWO52_000241"
     CDS             47591..48004
                     /locus_tag="IWO52_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0354539.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48127..49308
                     /locus_tag="IWO52_000242"
     CDS             48127..49308
                     /locus_tag="IWO52_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0354540.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49697..49770
                     /locus_tag="IWO52_000243"
     tRNA            49697..49770
                     /locus_tag="IWO52_000243"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49731..49733,aa:Arg,seq:cct)
     gene            complement(49807..50007)
                     /locus_tag="IWO52_000244"
     CDS             complement(49807..50007)
                     /locus_tag="IWO52_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0354541.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(50312..51544)
                     /locus_tag="IWO52_000245"
     CDS             complement(50312..51544)
                     /locus_tag="IWO52_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0354542.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            51798..52367
                     /locus_tag="IWO52_000246"
     CDS             51798..52367
                     /locus_tag="IWO52_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0354543.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(52545..52985)
                     /locus_tag="IWO52_000247"
     CDS             complement(52545..52985)
                     /locus_tag="IWO52_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0354544.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            53143..53907
                     /gene="map"
                     /locus_tag="IWO52_000248"
     CDS             53143..53907
                     /gene="map"
                     /locus_tag="IWO52_000248"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0354545.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            53939..54862
                     /locus_tag="IWO52_000249"
     CDS             53939..54862
                     /locus_tag="IWO52_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0354546.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            54938..56077
                     /locus_tag="IWO52_000250"
     CDS             54938..56077
                     /locus_tag="IWO52_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0354547.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            56070..56870
                     /locus_tag="IWO52_000251"
     CDS             56070..56870
                     /locus_tag="IWO52_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0354548.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            56870..57697
                     /locus_tag="IWO52_000252"
     CDS             56870..57697
                     /locus_tag="IWO52_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0354549.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            57675..58409
                     /locus_tag="IWO52_000253"
     CDS             57675..58409
                     /locus_tag="IWO52_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0354550.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            58509..59375
                     /gene="rfbA"
                     /locus_tag="IWO52_000254"
     CDS             58509..59375
                     /gene="rfbA"
                     /locus_tag="IWO52_000254"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0354551.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            59389..59961
                     /gene="rfbC"
                     /locus_tag="IWO52_000255"
     CDS             59389..59961
                     /gene="rfbC"
                     /locus_tag="IWO52_000255"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0354552.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            59983..61011
                     /gene="rfbB"
                     /locus_tag="IWO52_000256"
     CDS             59983..61011
                     /gene="rfbB"
                     /locus_tag="IWO52_000256"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0354553.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            61109..61960
                     /gene="rfbD"
                     /locus_tag="IWO52_000257"
     CDS             61109..61960
                     /gene="rfbD"
                     /locus_tag="IWO52_000257"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0354554.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            61995..64028
                     /locus_tag="IWO52_000258"
     CDS             61995..64028
                     /locus_tag="IWO52_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354555.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            64072..65352
                     /locus_tag="IWO52_000259"
     CDS             64072..65352
                     /locus_tag="IWO52_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0354556.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCRSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            65562..66368
                     /locus_tag="IWO52_000260"
     CDS             65562..66368
                     /locus_tag="IWO52_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0354557.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            66380..67600
                     /locus_tag="IWO52_000261"
     CDS             66380..67600
                     /locus_tag="IWO52_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354558.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            67590..69176
                     /locus_tag="IWO52_000262"
     CDS             67590..69176
                     /locus_tag="IWO52_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0354559.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            69215..71353
                     /locus_tag="IWO52_000263"
     CDS             69215..71353
                     /locus_tag="IWO52_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0354560.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(71721..72704)
                     /locus_tag="IWO52_000264"
     CDS             complement(71721..72704)
                     /locus_tag="IWO52_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ0354561.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            73082..74473
                     /locus_tag="IWO52_000265"
     CDS             73082..74473
                     /locus_tag="IWO52_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0354562.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            74489..75529
                     /locus_tag="IWO52_000266"
     CDS             74489..75529
                     /locus_tag="IWO52_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0354563.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            75548..76633
                     /locus_tag="IWO52_000267"
     CDS             75548..76633
                     /locus_tag="IWO52_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0354564.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            76666..77628
                     /locus_tag="IWO52_000268"
     CDS             76666..77628
                     /locus_tag="IWO52_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0354565.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            77621..79048
                     /locus_tag="IWO52_000269"
     CDS             77621..79048
                     /locus_tag="IWO52_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0354566.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            79050..80120
                     /locus_tag="IWO52_000270"
     CDS             79050..80120
                     /locus_tag="IWO52_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354567.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            80107..81528
                     /locus_tag="IWO52_000271"
     CDS             80107..81528
                     /locus_tag="IWO52_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0354568.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            81541..82548
                     /locus_tag="IWO52_000272"
     CDS             81541..82548
                     /locus_tag="IWO52_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0354569.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            82560..83564
                     /gene="neuB"
                     /locus_tag="IWO52_000273"
     CDS             82560..83564
                     /gene="neuB"
                     /locus_tag="IWO52_000273"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0354570.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            83561..84709
                     /gene="neuC"
                     /locus_tag="IWO52_000274"
     CDS             83561..84709
                     /gene="neuC"
                     /locus_tag="IWO52_000274"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ0354571.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            84682..85359
                     /locus_tag="IWO52_000275"
     CDS             84682..85359
                     /locus_tag="IWO52_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0354572.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            85349..86491
                     /locus_tag="IWO52_000276"
     CDS             85349..86491
                     /locus_tag="IWO52_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ0354573.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            86504..87481
                     /locus_tag="IWO52_000277"
     CDS             86504..87481
                     /locus_tag="IWO52_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0354574.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            87481..88389
                     /locus_tag="IWO52_000278"
     CDS             87481..88389
                     /locus_tag="IWO52_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0354575.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            88390..89142
                     /locus_tag="IWO52_000279"
     CDS             88390..89142
                     /locus_tag="IWO52_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0354576.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            89147..90094
                     /locus_tag="IWO52_000280"
     CDS             89147..90094
                     /locus_tag="IWO52_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0354577.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        30049 a        14583 c        18620 g        26929 t
ORIGIN      
        1 tactttgtct ttttacactt aatatttttt tgccaatttg gaatcaggat gttttgataa
       61 ctatcgtttt caattgatgg aagctatatt gtttgctatc agtgaaataa ttatatggta
      121 aacagtgttc tttttcttgt ttccattata aaaatcgtat cgggattttt cgttctttta
      181 aattgaacat ttctatctat ccgcgtgtta tcaacaaaac aaaatcaggt aaatagttga
      241 cgaaacagaa ataaatacat agtattatgt tttgaagcct aagtacaata gtctatagaa
      301 aaggagcgat accattggca aaacgcattg accgaattta tatgtatgtc aaagaaaaaa
      361 ccgctcattt aacaaaggca gaatatgatc aaggagtaac aacacaggaa attgctgaag
      421 tgttagggat tcagcgaaca aatagcagta aagatctgaa tcagctagtg cgcgaaggaa
      481 aactgataaa gacagacgga cgaccagtac gttacatata tcaaacactc gttacgcacg
      541 taaagccagt tacaaaacat gtagtcagtt acaaagaaac gccggtctct gaagaaaaaa
      601 atacactcca aatagatact aaggatatct ttgcaaaaat cattggagct aacggcagca
      661 tgaaaaattc agtggaacag gcaaaagctg cgattttgta tccgccaaga ggattaaatt
      721 gtttgattac aggacctacc ggatcaggaa agacttattt tgcacacgcg atgtatcatt
      781 ttgcaaaagc gcatcatgta atagatgagg aaacggaact gattgttttc aattgtgctg
      841 attatgccaa taaccctgag cttttgatga gtcatttatt tgggtacgca aaaggtgctt
      901 ttacaggcgc agatgaagaa aaaatgggga ttatcgatca agcggatggc ggtatgctgt
      961 ttttggatga gattcaccgg ttgccgcctg aaggtcaaga aatgatcttt tactttatgg
     1021 atcatggaac ttacagtcgt cttggggaaa caacgaaatc ccatgaagca aatgtacgga
     1081 tcgtaggtgc gaccacagaa gacccaggtt cttctctatt agaaactttt gttcggcgta
     1141 tcccaatcaa tatcaaactt ccggcttttg aaaaacgtcc tgcaaatgag aaagtcgatt
     1201 tagtaaaaat catgattgcc cacgaagcaa accgcacgca gcgaaagatt tctttgacag
     1261 aagatgtagc aaaagcattg atcggcagtg tgacctatgg aaatatagga caattgaagt
     1321 caaatatcca gttagtatgt gcacgaggat ttctaaatca tatgcaatca cccgaaatct
     1381 cgatcacaat cgatgatttg acagaaggga ttcgtagcgg attgattcaa ttagcaagca
     1441 accgcagtgc aatggcagag ctttctaaaa tattggagcc aaagataact gtagcaccaa
     1501 atgatacgat catgaaaatc caatcggact cctatgagtt accttataat ctatatgata
     1561 tcatcggaga taaagcagct ttactgaagt cggatggttt ggatcaagaa gcaatcaatc
     1621 atttcatttc tacagatatc aacatccatc tgaagtcttt ctataaagac catggttttt
     1681 cttttaatgc agacagcaaa ttagcagaat ttgtagatcc gaaaatcata gaagtgacca
     1741 atcagattta tgtaatggtc aaaagatctt taccatatga gtttcagcaa aactttattt
     1801 atgctatgag cttgcatatc agttcttttt tgaaaaaaat caacttagga gaagaacggc
     1861 atacgaatga taacatccgt gagatggctt ttgattttcc agatgaatat gctgttgcga
     1921 aagaagtgcg tagttatatc gaaacgtatt ttcaagtgga gattccagaa agtgaagact
     1981 attacttgac cgttctactc gtctcgttac gggcaaatca agcaagcggc agaatcggtg
     2041 tagtcgttgc agctcacgga aacagcacag caagcagtat ggtccaagtt gtccaacagt
     2101 tgttagatgc agagaacgta cgagcagtag acatgccttt ggatatggat ccgaaaacag
     2161 cattggaccg aattgaacgc aatgtacaag aagtagatga aggaagcggg gtcatactct
     2221 tagtagatat ggggtccctt gcgtcgttta attcgcaaat tcaaagagac actggaattc
     2281 cagtacgtac tgttgatatg gtaacaactt ctctagtttt ggaaactgta cgaaaggtct
     2341 cagtattagg gacagatcta gatatgttgc atgaatcatt gaaaaatttt cgaggttacg
     2401 cagaaatatc ttctgtagaa tctgttcagg aagcaaaacc tattcataag aaaaaagctg
     2461 ttttggctat ctgcgcatct ggcgaaggta cagcacaaag aatcaaagaa ttgatcgaac
     2521 gagcggtaaa caagcgtcaa gaaacagaat tagaagttct agctttgtcg attgtggaag
     2581 tgaaagaaga actgccagtt atccaaaaag aatatcagat catagctact actgggatta
     2641 ctgatttgaa tatccaagcc ccatttattc ctttagaacg ttttattgat caaaatatcg
     2701 aagtcatttt ggatcagttg ctgatggaat cagaattgga ggaaactgaa ttcatctccc
     2761 tagatgaaga gtcagctaaa aatacgtgtg ttgagtttat ctctgataac ttcatcttta
     2821 ttaatggtag taagttaatc gatccaatgt ggcaattttc tacacaaatc tctcagacga
     2881 cgggaatcgg cgatgaagaa tatggattca aaatcaacct agtgatgcac acagcaggaa
     2941 tgatcgaacg tattattcgg aatgaaccat tgactgtgga agaaaacgaa ttgacaaata
     3001 caacgaatga tcctctttat agccaattgg cagcctcagt tgttctgttg gaagatcaga
     3061 taaaagtaaa agtgccgata gaagaaatgt attatctgct acgtctagtc cataatcagt
     3121 tagataaaaa agaatacact gtgccatgaa tacacaaaaa cgatacactt ttatagtgta
     3181 tcgtttttgt gtattggtta agaatccttt aaattcaatt agtgtatcgt aaaaaaagtt
     3241 ggcacgcttt ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac agaaatgata
     3301 ggggtttctg tgaagtagtg aggaagaagg agttttgcag atggatattc gattggcacg
     3361 tatagatgat cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc aaacaggtgt
     3421 tgaacgtatc attgtgatca gcgatgtagt cgctcaagat aaattgagaa agtttctact
     3481 aaaagaagct gcaccgccgg ggatcacagc aaatgtaata accgtgcaga aaatgattca
     3541 aatctatcat agtggattat tatcacgagc aaaagtgatg cttttattca ccaatcccca
     3601 agatgttgaa gcggttgtcc gctgcggtgt acagttgaag tcgttgaata tcggcggtat
     3661 gagttttact gaaggcaaat caatgattac caattttgtt tcagtaaatc aacaagatat
     3721 tgattccttt cgttacttga acagtcaaga agtggagtta gagattcgaa aagtacctgc
     3781 tgacaggaaa atccttttga tggaactact agcgaaaatc aaaaaggtgt agcaagaatg
     3841 gttgttggaa aaggtcaaat aattctgttg taatggttag caattattaa tatttttgaa
     3901 tttgttacaa caaaagtaaa taggaggtat aaatatggta gggattatcc tagcaagtca
     3961 tggacaattt gctgaaggaa tcttacaatc cggttcaatg atctttggcg aacaagaaaa
     4021 cgtcaaagcc gtgattttga aaccaagtga aggtccagat gacttgagag ccaaattgga
     4081 agaagcggtt gcttcttttg ataatcaaga cgaagtgtta ttcttagttg atttatgggg
     4141 cggaacaccg ttcaatcaat caaacacatt attcgaagag cacaaagata aatgggcaat
     4201 cgtcagcggt ttgaatttac caatgttgat tgaagcctac gcatcacgct tttcaatgga
     4261 atctgctcat gaaattgcag cacacatcat tgaaacagca aaagacggtg taaaagtaaa
     4321 accggaagaa ttagaaccgg cagaagcacc gaaagcagca gttgaagacg cacaacctaa
     4381 aggagcactt ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg tattagcacg
     4441 tgttgactca cgtctgttac atggacaagt tgcgacagct tggacaaaag cagtgcaacc
     4501 aaaccgtatt atcgttgttt cagatgcagt atcaaaggat gatctgcgta agagactaat
     4561 cgaacaagct gctcctccag gtgtaaaagc aaatgttatt cctattagca aaatgatcga
     4621 agtagcaaaa gaccctcgtt ttggaaatac caaagcattg cttttatttg aaaatcctga
     4681 agacgtattg acagctgttg aaggtggcgt agacatcaaa gaattaaatg ttgggtcaat
     4741 ggcgcattcg gtcggtaaag tcgttgtaag taaagtattg tctatgggac aagaagacgt
     4801 tgaagcgttt gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta aagtacctaa
     4861 tgattcacgc gataacatgg atgatatttt gaaaaaagca aaagctgaat tagcaaaagc
     4921 gtaataaata atattaaaat ttataggagg cttatcatgt ctattatttc aataatttta
     4981 gtcgtattag ttgcctttct agctggtatg gaaggtattc ttgatgaatt tcaatttcat
     5041 caaccactag tggcatgtac attgatcggt ttagttaccg gtaacctcga agctggtatc
     5101 gtacttggcg gtacgcttca gatgatcgct ttaggttggg caaatatcgg tgccgctgta
     5161 gcaccagatg ctgcattagc atcagtagca tcagcaatta ttttagtttt aggtggacaa
     5221 ggtgttaaag gtgttccttc tgcgatcgcg attgccgttc ctttggctgt tgcagggttg
     5281 ttcttaacaa tgatcgtacg tacaatcgct gttccaatcg ttcatttgat ggatgcggct
     5341 gctgaagaag gaaacatccg taaggttgaa atgtggcaca tcattgctgt atgtttacaa
     5401 ggggttcgta tcgcggtccc agcagcagcg ttgctattta tcccagcaca aactgtgcaa
     5461 tcattccttg aatcaatgcc agcatggtta acagatggta tggctatcgg tgggggaatg
     5521 gtcgtagctg taggttacgc tttggtaatc aacatgatgg ctacaaaaga agtatggcca
     5581 ttcttcgtta ttggtttcgt tgtagctgca atttctcaat taacattgat tgctctaggt
     5641 gctttaggtg ttgcattagc tcttatttac ttgaaccttt ctaaaatggg tggttcatca
     5701 aataatggag gcggaagcaa ctctggtgac cctttaggcg acattttgaa tgactattaa
     5761 tcttgaagga ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa aaagatcgtt
     5821 tagctgttgc gtggcgctct accttcattc aaggttcatg gaactacgaa cgtatgcaaa
     5881 acggtggctg ggcattttct atgattccag caatcaaaaa attatacaaa acaaaagaag
     5941 aacgttctgc agcattaaaa cgtcacttgg aattctttaa tacacaccca tacattgctt
     6001 ctccaatttt aggggttaca ttggctctgg aagaagaacg tgcgaatggc gcgcctgttg
     6061 atgatgtagc gattcaaggg gttaaagtag gtatgatggg tcctttagct ggtgttggtg
     6121 acccagtgtt ctggtttaca gtacgtccaa tgctaggtgc actaggtgct tcattagcta
     6181 tgggaggaaa tatccttgga ccaatcatct tcttcttagg ttggaacatt attcgttggg
     6241 cattcatgtg gtatacacaa gaatttggct acaaagctgg ttctaaaatt actgatgacc
     6301 tttctggcgg attgttacaa gacgtaacaa aaggtgcttc aattttaggg atgttcgtat
     6361 tagctgccct tgtacaaaga tgggtatcaa tcaaattctt accagttgta tcaactgtaa
     6421 aattagataa aggtgcttac atcgaatggg ataaattgcc ttcaggtggt gaaggaatca
     6481 agagtgcatt tgaacaagtt aataatggct tagccctttc tcctgaaaag gtaacaacat
     6541 tacaagacaa cttagaccaa ttgattcctg gtttagctgc attaggatta acattgttat
     6601 gtatgtggtt gcttaagaaa aaggtttctc caatcgttat catccttggt ttattcgtag
     6661 tcggtgttgt tggacacgtc atcggacttt tataatccat aaaaatatag ctcgggcgtt
     6721 gtcccgagct ttttttatat agtatactgt tcataaacaa atatctggag ggagaaaatg
     6781 gttcaatcaa tcaatacaaa agtagacttg gtaattgatg ctacttcgtt tacgggtctg
     6841 acagattacg ggcaaatcat gataggtgat aatggttttg agttttacca tgcgcgagat
     6901 agacgcaaat atatccaaat tccatgggaa gaagtggatt atgtactagc atcagtcatg
     6961 ctaaagggaa aatggattcc tcgatatgcc attcaaacga aaaaaaatgg tacatacaca
     7021 ttttcttcca aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt agatgaagat
     7081 agaatggtac aatcattgag tttctttgat gtagtgaaac gtggtattcg tagtatgttt
     7141 aaacgcaagt aaaaaataaa gaaaacttct aagaggaggt tttctttatt tttttacttt
     7201 gcagaagaaa atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc ccatatgcta
     7261 aaattgatta gcaaagaagg aggttatatt atgagttggt tatggacatt gattgttgga
     7321 gccgtgatcg gagcaattgc aggtgcgatc accagtaaag gaaattcgat gggctgtgta
     7381 acgaatatca ttgctggact agtcggttca agtattggac aagcgatttt cggcgaatgg
     7441 ggaccaaaag ctgcaggtat ggcacttatt ccatctattt taggcgcagt gattttggta
     7501 gcagttgtct catttttctt tggaaaacga tcataagttt ttacaaaata gtaaactaaa
     7561 aggagttgca gcgagactgt gactcctttt tggctttatt tatttaaaat aaaaagaagt
     7621 tttccggaaa tttttattta actgtcgtgt acgaagaaaa gtttgctata atggaaaaaa
     7681 gcgtgcaaag aaagagggag tttcattatg atgctacgtg tagaaaaatt gcgcaaaaaa
     7741 atgcaagaag aaaatttgga ttcatttttg gttaccagtc cgtacaatct tcgttactta
     7801 acgaacttta caggtacgac aggattagct gttattactt tggaaaaagc attttttatt
     7861 actgattttc gttataccga gcaggcggca gcgcaggctc aagggtttga aattattaaa
     7921 aatgtcggac ctatttttga tgaggtagca aacttagtac gcaaagaaga actgcactca
     7981 ttagcttttg aggaaacaac agtctcattt ttagaatatt cggttttaga agaaatcatc
     8041 gatgcggaat tagtacctgt ttcacagatg atcgaagaac tcagagaagt gaaagatgaa
     8101 gaagaaatcg ctattatcga aaaagcatgt catattgcgg atatggcata tgatcacatc
     8161 ctgaaaatga tccaaccggg catgactgag attgaagtcg caaatcaact agacttttat
     8221 atgcgttctc ttggcgcttc aggtgtttct tttgatacga tcgttgctag cggattgcgt
     8281 tcggctatgc cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga cttgatcaca
     8341 ttggactttg gttgctatta cgaagggtac gtttccgata tgacacgtac attcgctatt
     8401 ggtgatcctg gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc tcaattagca
     8461 gttattgatg ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt tgctagagat
     8521 tatatttcaa aacatggtta cggggaagca tttggacatt ctacaggaca tgggatcggt
     8581 ttagagatcc acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt tgtaccagga
     8641 aatgtgatca ctgatgaacc agggatctac ttaccaggaa tcggtggagt acgtattgag
     8701 gatgacttgt tgatcacaga agatggaaat cgtgtgttga ctcgttcacc aaaagaattg
     8761 atcatcttat agtttcatca atagaagaag taagcgtaaa aatcagtgtt cacagacttt
     8821 gttatagaaa atcactcgat ttaatgatac actatagaag aacttttaag ctgtaagaga
     8881 taataaagtt gggtgttttc tccaattcat agcagtttgc agctgatcgg cggcagtttt
     8941 cttaccacgt gatcactatg ccgcatcata gagaattcgg atgtatctcg tgcttaggca
     9001 aagggaggaa taaataaatg gcagatgaaa aaaatttgat tttaaacgca aaccaagatt
     9061 taggagagat cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt gcagcttcta
     9121 aagtagaagg cgtatacgga atgcgcggaa cttttgccag caatgtcact gaattattag
     9181 gacgtgcagc tcacggaaaa ggtgtttact tagcaaacga ggaagatggc ttgaaagtag
     9241 atctttactg ttatctagaa tatggtgttt cagttcctaa agtagcaatg gaaatgcagg
     9301 aacgggtcag ccagcaagtc cttttcatga cggatatcga tttatctgaa gtcaatatcc
     9361 atgtagtagc tgttgttcct gaaaaattag aacaaccaac aatcgaagaa ctttttgaag
     9421 acgaagagga agagaatgaa taaagaatta tcaagacacg agattcggga aatggcattg
     9481 caggctttat ttccattaga cttcaatgca gacctaacaa aagaagatgc gattttcaac
     9541 gcaatcgaat tagaccaccg cgacatgatc aacgaagatg aatcagagtt tgttcctgtt
     9601 tatcttgata cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga taaagtgatt
     9661 gaaaaacatt taaaaaacaa ttggcacatc aatcggatct caaaaatgga tttggtgatc
     9721 ttgcgtatcg cgatttttga aatgacttat gtgacagatg taccagcggc tgttgcttta
     9781 aatgaagcaa tcgagttggc aaaaacattt agtgatgacc gttcacgtaa attcgtcaac
     9841 ggtgttttat ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct agcttttttc
     9901 tatgaatcat cataaacctg ttataaatcg ggaaaattct atggtagaat gaaaacacaa
     9961 aaaatatctg ggagtggtta gatggcagaa ttgattaacg gaaaagaatt agctgaaaaa
    10021 atgcaggcag agattgctga aaaaatccaa aaattgaaag ataatggtat ccatcccgga
    10081 ttagtcgttt tattggtagg ggaaaatcca gccagccaaa tctatgtaag aaataaagaa
    10141 cgtgtagcaa aagaaatcgg aatccattca ttggtagagc gttatcctga gacgatttct
    10201 gaagacgaac tacttgcaga aattgaaaaa tacaatcaag atcctagttt tcatggtata
    10261 ttggtccaat taccattgcc aaagcatata gatgaagaaa agatcctgtt agctatccgc
    10321 cctgaaaaag acgtagacgg ttttcatccg atgaatttag gcagactcct tgcgggtaaa
    10381 ccggacaaga ttccttgtac tccatacggc atcatgaaaa tgtttgaagc ttattctatc
    10441 gatccggcgg gtaaacgagc agtagttatc ggacgaagca atatcgtcgg taaaccgatg
    10501 gctcagctac tcttgatggc agatgcaact gttactgttg ctcattctaa aaccgaaaat
    10561 ctggctgaac tggcacaaga agcagatatt ttagtcgtag caataggacg tggacatttt
    10621 gtcacaaaag aattcgtaaa accaggtgct gtagtgatag atgtagggat gaatcgtgat
    10681 gctaacggta agctgatcgg tgatgtgaaa tttgatgaag tggagcctat tgcaggctgg
    10741 atcactcctg tgccaaaggg tgtagggcca atgactatca cgatgctgat gtatcaaacc
    10801 gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg cacaagaata tttaactgtc
    10861 actacattaa cgaagtattt gaaaagaaaa tttgatgccg atccttattt agaacgcgta
    10921 tatttaacag gcgaaatatc gaatttccgt ttacgtgcca atgcccatca atatttcagc
    10981 ttaaaagatg accatgcaaa aatatctgct attatgttca aaggagcatt ccaaaaatta
    11041 cgattccagc caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc attatatgaa
    11101 gcgggaggtt cttatcaaat ctatgtagag cacatggagc ctgatggaat aggtgcttta
    11161 tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt tcaagggcct
    11221 aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc aagcggagcg
    11281 gttatccgcg atatcatcac gacggtgaaa agaagatatc caattgtcca attggttttg
    11341 ttcccaacag ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa tatccagaga
    11401 gtagaagaga tgggcaattt cgatacaatg atcatcggtc gtgggggtgg ttcgattgag
    11461 gatttatggc cgtttaatga agagcgggtt gctcgggcga tttatcaagc taatacgccg
    11521 gtgatttcat ctgtaggaca tgaaacagat accacgatcg ctgatttagt agcagatgtt
    11581 cgggctgcca ctccaacagc agctgcagaa ttagctgttc ctgttttgtc agaagagttg
    11641 atgcgcattc aagaacggca agctagattg cagcaggcgt ttgtacgcca gatccagcgc
    11701 aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc tgaaagattg
    11761 tatgaaggtc agaccatcaa attagaccag ttgaaacagc gattatatca agctacacag
    11821 caaatttatc atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt ggaacaagtg
    11881 gctccgcttt atcgagttaa agcagctcgc caagaaactg cttatctaga aaaacgctta
    11941 gaagagaaaa tccaacaata tatgaaacaa cagcagcagc gttttgctca agctgttcaa
    12001 tctcttgatt tgttgagtcc attgaaaatc atgggaagag gctacagcta tacgacgtta
    12061 aatgatcaag tagtaaaaag tgtcaccgag attcatcaag gggatttgct gacgatccat
    12121 tatcaagatg gaatcgttaa aggacaagta caagagatag acaaaggaga ataaaaatat
    12181 ggaaaatcca acttttgaag agtcactgca agaattagaa aaaattgtca tgcagttaga
    12241 acaaggagac gttccattag aatcagctct agattcgttt aaacgaggaa tggaattaag
    12301 caaacattgc caagatacat tagctaaagc agaagagacc ttgacgaaaa tgatgacaga
    12361 atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga ttaactgact
    12421 tttcagctat tcatctacct gctgtagaaa aagaaatctt gtctttttta gatgagcata
    12481 caacggatag aggactctat gatgcgatga gttattcagt gaaagcaggc gggaaaagaa
    12541 tacgtccctt attactttta acggccgttg cttcctttga tgagcctatt gatgttcccg
    12601 tctatcaagt agcagctgct ttagaaatgg tgcatacgta ttccttgatc cacgatgatt
    12661 tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc taccaatcat aaagtattcg
    12721 gcgaagcatt agctatattg gccggagatg gtttgcttac aggagcattc cagctaatca
    12781 gtatggcaca cttaggtaat tctcctaagc ttctgctgct tcagcaatta gctgtttgtg
    12841 cgggatcaca aggtatggtt gctgggcaag ctgcagatat cgagggcgaa tcgaaaaagc
    12901 tttctttaga agaattagca tttattcatg aaagaaagac agggcatttg attcgttatg
    12961 ccttgctggc tggtggcata ttagcgaagc aacctgaaga aatcttattg ctacttcaac
    13021 gattagccga acatttaggt ttagcttttc agataagaga tgatctactc gatgtgattg
    13081 gtacaacgaa aacattaggg aaaacagctg gtaaagatga acgtatggaa aaaaatactt
    13141 acccgcgttt attaggccta gaaaagacac gtgaggcgtt ggagattgag ctgttatccg
    13201 ccaacaagat catagacaaa ttagaagagg acgttttttc ttttgatggc gggctgatgc
    13261 gccagatgat caagcaattt gatgtggaga aatgagggaa agaatggaaa aagaacgcgt
    13321 agatgtatta gcggtcaaac aaggattatt tgaaacaaga gaacaagcca aacgttcagt
    13381 gatggctggt ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg gagaaaaaat
    13441 tccagtgagt agtgaattaa gagtcaaagg taaaaaactt ccttatgttt ctcgaggggg
    13501 attaaaatta gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa aaacattgtt
    13561 agatattggt gcatccactg gcggatttac tgatgcagct ttgcaaaatg gcgcaaaaat
    13621 gagttatgct ttagacgttg gctacaatca gctagcttgg aaaataagac aagatcctag
    13681 agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa cccgaagact ttaccttagg
    13741 aattccagaa gttgctgtta tcgatgtttc ttttatttca ctaaaactga tgctgcctcc
    13801 attacatgct attttaaaag aagagggaga agtgattgct ttaatcaaac cccaattcga
    13861 agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga cccatcaaaa
    13921 agttttagaa gacattttgt catttgcggc acaaggtgga tatgatgtgc tggaattaag
    13981 ttattctccg atcacggggg gagaaggaaa tatcgagttc ttggctcact tgaggaaagt
    14041 acccgaaagc ggaacaatca attcagctat taatatggca gaagtggttt caaatgcaca
    14101 cgaacagttt gaccataaat aaattgtaac tgtgaaaatc aagtgagaag tatgcggatt
    14161 cttctttatt ataccaagga aataagctat cattgattaa gaagaatatg ctaaggagcg
    14221 aagacgtaat gagaaaaaaa gatcggcatc gtttgattac tcgattgttg agtgagcaag
    14281 atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata tctgttactc
    14341 aagctacgat ttccagagat ataaaggaac tgaaactgat caaggtaccg gcttcagatg
    14401 gcggctaccg ttatagcata ccagctgaaa caaacgaaga tgtaaatact aaattggaaa
    14461 aactattaaa agacgctttc gtggcagttg accaaatgga aaaatttgtt attttgaaaa
    14521 cattgccggg aaatgcttcg gcagcagcaa atttgattga taaacgttac aagaaagaat
    14581 tattttcgat tatcaatgat gacgataatg ttttgatgat tacacgaact gaagaccagg
    14641 cagttttatt gaagaaagac ttcctgcatt acctttaagt tgaggagcga atgtgatgct
    14701 acaagaaatc agtattacta actttgcgat tattccggaa ttgcggcttt cttttcacga
    14761 aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa tccattatta tcgatgcttt
    14821 aggtctgtta gcaggaggaa gaggttcaag cgattatata cgtcaaggag cagaaaaatg
    14881 tgtgcttgaa gggttgtttg aactaccaaa acaagaagga ttctctgaac tgatggttga
    14941 attagggatc gaaacagatg aggacaatct gattgttcga cgggatatgt ccttaactgg
    15001 aaaaaatgtt tgtcgagtga atggtcatat tatcacatta gctaatctga gaagaatcgg
    15061 cagttattta gtagatatcc aaggacaaaa cgaacatcag gaactgctcc agcctgaatc
    15121 acatcttgct ttattggatc gttttggaga tgtagctttc caacagaaga aaaagagtta
    15181 tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag tgcagcaaaa
    15241 tgaaaaatcc tatgttcaga gaatagatat gctccatttc cagcaagaag aaatagctgc
    15301 tgctgatttg caagtcggag aagaagagaa attaaaagaa gaaagagaaa aacttagcaa
    15361 ttatcaaaaa attgctgatg gattagctgc aggttacgga gcactgacga ataatgaaca
    15421 aaacagtgta gatggtgttg gcttggccgt ttcagaactg caagggatcg cccatctaga
    15481 tgtggagtat gaagcaattt atgaaaatat ccaaagtgct tactatttgc tgcaagatgc
    15541 aatcggcgat atgagtcgtc agattgattt attggagtta gatgagagtc gtttagaaga
    15601 agttacgcaa cgtttagaat taatccgaca gttgaaacgt aaatacggcg aatccattga
    15661 gtcaatattg gcttactatg acgaaatcac cgaagagcta gcttcttctg atttttcaga
    15721 aggtcaattg gacaaaatga aagaggaact agagcaaaaa gagcttctac ttcagcaaca
    15781 agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa aatccattct
    15841 gcatgaattg aaaagtctgt atatggaaaa cactgagttt gaggtacgtt tcttaaaaga
    15901 agaaaataga caattgaatc atgatggatt tgaccaaatc gaattttaca ttacgaccaa
    15961 tccaggagag ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac tgtctcggat
    16021 gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta ttatttttga
    16081 tgaagtggat acaggtgtga gcggaagagt tgcacaggcc atcgcagata aaatttctca
    16141 aatatcgaaa tactctcaag ttttgtgcat cactcatctg cctcaagttg ctgcagtcgc
    16201 cgactatcaa tattatattg tgaaagcagt gattgatgga cgtacgcaaa cttctgtttc
    16261 agagttgaaa acaaaagaaa gagaagaaga aattgcacgt atgctggctg gaagtgagat
    16321 tacaaaactc acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa ggacctgagc
    16381 cacctcgcag tggttcaggt ccttttcagc agctgatagc tatattttat ttttacatga
    16441 cactcattac agcagtcaat acaacagatc caatcgttaa aatcagcatt gtccaaatga
    16501 caaattttgt tactttagca aaagtacttg tttgtttttt ttcacgcatt gttttcaacc
    16561 tcgtcattaa ttagttgatt caagtgtaca atcttttttc catttccaca agagggcttt
    16621 tttataaaaa cttccctttt tttaaataat aggtagttac gatacaaata aataatgtta
    16681 atagaactag ccatataaag gcatcatccc tttcagcaat aggtaactta atgttcattc
    16741 catagatact gccgacgatt gtcggtatag ttaaaataat cgttaaagaa gttaaaattt
    16801 tcataatatt attcagatta ttcgatacga ttgctgaaaa agtctgattc atttgttcta
    16861 ctagttttaa ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc aatacatcac
    16921 gtaaatgatt tttcaaagca tgattttctt ggaaaagttt attttgcgct agttctttca
    16981 atgcatctaa attagcagaa gtcgcttctc tgaaataaac gaggcttttt tggatatcca
    17041 tgatttgata aagttgtttg ttttcagtgg atacttgaag tttggcttcc aaagtatcgc
    17101 attgagattg aagcgtagct aaataactgt tgtaagagag tgtgacctgc caaatgaatt
    17161 gaaagaagat attgagatta ggcgaaatat cactttttgg aaaggtctgg ctaaagactt
    17221 tctttaaaaa cagtggttca tgattcgtaa ttgtgataag cttctttttg ggcgtcaaaa
    17281 taatagagag cgggtatgta tccacttgaa aataaccgct aggacttgtt ttagcaaaag
    17341 gatattgtaa caataataac gctggctttg tcaactttga ttgattgaat tcttctgtac
    17401 gggaattttc tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt tcaaattgat
    17461 gtgtcagctg atgaatctct tcttgattag gacgttctac acaataccag atcgtttggt
    17521 cagtttcctt tccggtatta cgcagtttct cattatctaa tgtaaaatat ttgatcatga
    17581 ttttcctcct gttgcctgtt tcttttatta taacggatag ggaattggaa tacatggaat
    17641 gtgtttgtaa gatatagaat ataactgcat aaatttaaat aaaatgaaac agttgtgtaa
    17701 agtatataca gtatacttga agcaacgata aaaaaggagt ttacgttcat gggcacattc
    17761 acaccactta tcttgttttg gtatatttac ccggtaatcg ttttgtttgg ttgccaattt
    17821 cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa aagcccctga tttggcgatt
    17881 ccttttttgt ggggcggtat gcatgcattg tctagaaata cagggacaat ttctatctta
    17941 ccgtttttct ttatttctgt tttgctaatg ggaatttgta ttgctgtttt ccaagcatat
    18001 tattatgaag aaattattta tccgcgttat ttcaagatga cttggcgttt ggtttttctg
    18061 ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca tactttctta cctatgaaaa
    18121 aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt tttaaaaaaa
    18181 tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa aaggtggtag aaagtggggg
    18241 attgtggtag actaatttta gttagtggag gaatgggggg ctttcaatgt tgatgggtga
    18301 attccaacat aatattgatg caaaaggacg tttaatcgtg ccctcaaaac ttcgtgaaga
    18361 acttggcgaa aagtttgttc tgacacgcgg attggatggc tgtcttttcg gctatccgat
    18421 gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg ccgttggcta aaaaagacgc
    18481 acgtactttt gtgcgattct tctattcggc tgccactgaa tgcgaacttg ataaacaagg
    18541 acgaatcaat atacccagca cgttaagaaa ttatgcagct ttgacaaaag gttgcgtagt
    18601 catcggtgta tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag atttttctgc
    18661 agcagcagaa gagaactttg acgaaattgc tgaaagtatg attgatttcg gactataaga
    18721 ataggaagag gctgactaat gactgaaaca tttcaacact atacagtcat gttgaaagaa
    18781 acggtagatg gattgaacat caaaccggat ggcgtttatg tagactgtac actcggcggt
    18841 gccggacata gtgaatattt attaagccaa ctaagttcta aagggcatct gtatgcgttt
    18901 gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta tgtcgaacaa
    18961 ggtatggtca catttatcaa agcgaacttc agagaattag aagaacgctt gagtgagcat
    19021 gtagataagg tagacggcat tttgtatgat ctaggggttt cctctcctca gctagatgaa
    19081 gcagaacgtg gatttagtta tcatcaagat gcaccgttgg atatgcgcat ggatcagtca
    19141 gctcctttat ccgcttatca cgtaattaat gaatattcct accatgaatt agtaaagatt
    19201 ttcttccgct atggcgagga gaaattttcc aaacaaattg ctcgtgaaat cgaacgagta
    19261 cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg aaatcattaa acaagtcatc
    19321 ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa gaatttttca agctgtgcgg
    19381 attgcagtaa atgacgaact cggtgccgaa gaggcctctt tagaacaggc aatccggtta
    19441 ttgaaagtca atggaagaat cagcgtaatc acattccatt cattggaaga cagaattgtc
    19501 aaaagtatgt ttaaagaata tagtacggta caagatctac caccgggact tcctgtcata
    19561 cctgacgaat tccaaccaga actgaaagtc attactcgta aaccaatcct tccttcagag
    19621 gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc agaaaaaata
    19681 agaatgaaag aggagtagaa tgatggcaga attaaagaaa atgcaagatt acgaatatga
    19741 tttacctgaa ttggtggatc agccggaaca acaaatgcct gattcttctt cgaatcaaga
    19801 aatcattctt ccacaatcac cggcaaaacg attaaagcag atttctgctt tggaaaaaat
    19861 tgctgtggca agcgtggcga ttgctattgt tgctttatgt gttttaacag ttatgctgcg
    19921 cacaaatatc agcggagctg aaaaagaaat cacgtcaata caaacagaaa tcaatgataa
    19981 aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta ctgaacggat
    20041 taaacaaatc gcagaagaaa aaggattgtc tattaatgac gacaatttaa ggaaagtgaa
    20101 gtaaatgagt cttaaaaata aaattcggcg gtttatggaa aagaaaaatc taaatccgat
    20161 gaacaatcgc aaaaaagtag gtatcatttt atttgctacg agtattggat tgttcttttt
    20221 atttgccgtc cgtttctcgt atatcgttat tggaggtcat gttgctggta cctcattagc
    20281 tgaaaaaaca aaacagttgt accaaggaag tgaagtagta aaagcaaaaa gaggaacgat
    20341 ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat
    20401 actctcaaaa acatatacct ctggggataa aaaactgtat gttgaagaaa agaattttga
    20461 taaaatagca gaaatcctgc ataagaatct ttctatagac aaaaaagatg cactgaatat
    20521 tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct atgggaaaaa
    20581 tatctcccaa gaaaccaagc aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg
    20641 tctttatttt gtcgaccatc aggctcggat gtatccaaat ggcgtctttt cttctcattt
    20701 tattggatac gcagttccag acaaagatga gaatggctta gttggtaaac taggtttgga
    20761 gtctgcttac aatgatatct tgagcggtaa agatggtaag attatttatc aaaaagataa
    20821 ttttcaaaat cctctgccag gtacagtggc agaagaagaa aaagctgtcg acggacaaga
    20881 tatctacacg actttagaca gcagactaca aagttattta gaaacactga tggatcaagt
    20941 aaatgaagaa tatcagccag aagaattgac agcagtcctg atgaaagcaa aaaccggaga
    21001 aatcttggca atgggacaac gacctacttt caatcctgaa acaatggaag gattgactgg
    21061 aaaagatgct atctggcgta atttcttggt acaagatagt tatgaaccag gatcaaccat
    21121 gaaagttttc actactgcag ctgctatcga agaaggagaa ttcaacgaga atgaaacctt
    21181 ccaatctgga aaaatccaag tagaagatgc tacgatcaat gaccatgact ttggtgaaaa
    21241 aggtgtgctg accatgcgtc aagctctttc atggtcaagt aacgtcggga tggtcatact
    21301 ggaacagcga ttaggcggac gatggtataa ctatttacaa aaactgggat ttggacaaag
    21361 tactcattcc ggattagatg atgaagtaaa cggtgcttta cctacattaa atattgtcga
    21421 ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc
    21481 tttcacttct attgcgaata acggtacaat gattcaacct cgatatatca gcaaagtggt
    21541 ggatccccaa actggagaag agcgaacaac tcaaacagaa gttctagggc aacctttttc
    21601 aaaagaaaca acagaaaaag tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta
    21661 cggtagtgct tatggtgttg tacctggata caatgtatcg gcaaaaaccg gaacagccca
    21721 aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt attcgattgt
    21781 ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga aacaccctaa
    21841 gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac gggcaatgga
    21901 ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag tatccgtagc
    21961 cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa gtggactgca
    22021 acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca acggtgatca
    22081 gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga tgcctgacgt
    22141 aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta ttgaagttag
    22201 ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa aaatcactga
    22261 agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa aagagcatgg
    22321 aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt gccgcaatgc
    22381 cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt gaggagggac
    22441 caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg tttctgatcg
    22501 gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact cctactcttt
    22561 tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat gattttataa
    22621 aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt ggacagattc
    22681 tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt gtattgaatt
    22741 tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc ttctggcttg
    22801 ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt gctggcttag
    22861 gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag tatgatgtat
    22921 taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac aacagaaaac
    22981 ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt ttagcggcaa
    23041 tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc tatgttatgg
    23101 aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa cgaattttta
    23161 aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg aagattgata
    23221 cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc gtttggtagg
    23281 agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct tgtcttagga
    23341 ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc acttgtcacc
    23401 gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct ttctttaggg
    23461 ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt ttcattgatg
    23521 gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca agaaatgggt
    23581 attccggtaa ttacggaagt tgagctagct tacgaagtat cggaatgccc aattattggg
    23641 attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact actgaatgca
    23701 ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc aagtggtgta
    23761 gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt ccaactcatg
    23821 ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga agcacatatt
    23881 gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca aaaaaacatg
    23941 acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga attagcgcaa
    24001 agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga cggtgtgtat
    24061 gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc tgaacttggc
    24121 gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc aaaattgtat
    24181 ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt accacatcgt
    24241 acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa agcaaccaat
    24301 attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt gttattggct
    24361 ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa aggaatcaaa
    24421 gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa aaaagcaggc
    24481 atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc attagaaaac
    24541 agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga tcaataccca
    24601 aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa atagaaaaag
    24661 tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag ggggacatat
    24721 ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg ctgaattcat
    24781 gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag gaatgccttt
    24841 ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca tcaagacggt
    24901 ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt ttcaaccaga
    24961 tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg cagccaaaat
    25021 gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca ataaattttt
    25081 aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct tttttcctga
    25141 aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata ctaaaaaatc
    25201 agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga tttttggcgg
    25261 tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg aatatattcc
    25321 ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga tcctaaataa
    25381 aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata tggcagaggt
    25441 catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg cagaattcac
    25501 agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg atcatcagac
    25561 taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag ataatgaact
    25621 aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag aattgcaaaa
    25681 gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac gtctttatga
    25741 cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa gaaaaaagaa
    25801 ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg gcaaaaggct
    25861 aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa agaagacata
    25921 acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga ggaacagcaa
    25981 gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca aaagaaaaac
    26041 ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag tcaacggcac
    26101 aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc gctgattttt
    26161 ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc aggcaataag
    26221 acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga aaatgtttgg
    26281 ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca gccaagaatc
    26341 gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt aacagaatat
    26401 aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga aaatggaaca
    26461 gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga agagttcaaa
    26521 gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc agaattacaa
    26581 aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct gatccaattg
    26641 aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa ccaaatgaaa
    26701 tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat ggaagttggt
    26761 atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca atcggaagaa
    26821 acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga ggcagaaacg
    26881 caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc aaatgattca
    26941 agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt aaatgaaaat
    27001 tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat ttatggtaga
    27061 attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc tagttgttcg
    27121 cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca ggaatgtatg
    27181 taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat atcgacagcc
    27241 aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga ggcattatcg
    27301 ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca gaagaaaaag
    27361 caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt gaagtagaaa
    27421 attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat gaagatgtca
    27481 gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa atcgtgtcaa
    27541 tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct cgtggaatga
    27601 tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg atcatccata
    27661 acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg attactcctt
    27721 tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg atcgtcattg
    27781 atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg aagtttacca
    27841 atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta ttaaatacgt
    27901 catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca gaacggacat
    27961 caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg aaagtcgatg
    28021 aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac aaagcaaaag
    28081 atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg acaggcggag
    28141 ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta aatgtaaaat
    28201 tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta ataagtatcg
    28261 tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct gttacgggtg
    28321 aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca tatgagagct
    28381 atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc ggagaaaatg
    28441 taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc ctgggaggaa
    28501 taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat caaagtcatc
    28561 ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa cgttaaaggt
    28621 gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa agctgaaaca
    28681 gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca gccagaagtt
    28741 ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga cggtgcagat
    28801 atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc accaatcgtt
    28861 gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg tccattcact
    28921 tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt gaaagaaaat
    28981 gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga taaaaagaca
    29041 ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt acaaggtatc
    29101 tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt gaaaacagtg
    29161 atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga agatcgcgta
    29221 gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat tgatggagct
    29281 gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga agcacaagat
    29341 gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct aggtacatct
    29401 atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg tatcgatgaa
    29461 tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca atcacctgct
    29521 caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga agaaaacagt
    29581 tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga tgattccaat
    29641 tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac aaaatctcat
    29701 ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag gagaggaaga
    29761 gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt cgtgtgctct
    29821 tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg ttgaatccgc
    29881 tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc gagtagataa
    29941 attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc atttaattgg
    30001 taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact ttcatgcgtt
    30061 ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat tatcctgttt
    30121 tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag atgagctttt
    30181 accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt tgatgactat
    30241 ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc tgaaagaatt
    30301 gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat taagtatggg
    30361 gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc gtgtaggaac
    30421 agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac agaggagcaa
    30481 ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat tatcctgaaa
    30541 caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa tcaactcatt
    30601 ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt cctgtaacgc
    30661 atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa tcacctacaa
    30721 aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa gcaaatcttg
    30781 ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg attgctaaac
    30841 gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa aaacaagcga
    30901 gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat atcaaacgtg
    30961 ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt tcaaccgctc
    31021 aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga tttatactat
    31081 tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta tcttgatggt
    31141 atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac gattattagc
    31201 taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga ttggaccctt
    31261 ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag gtttagcaca
    31321 gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa atctttattc
    31381 tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact ttcgggcaga
    31441 tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag gacaatatgc
    31501 accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa cactgattcg
    31561 acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag aaagaaaaag
    31621 atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa cagcattgta
    31681 tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc ttggaacttt
    31741 agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg gtgatcgctg
    31801 gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa caaagattgg
    31861 aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc cgaaagatag
    31921 ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga tctctgagat
    31981 ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag taaaagtgaa
    32041 ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta tttcagtcag
    32101 aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag aaaagtacag
    32161 ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat ggctttaact
    32221 ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa tcaagacgaa
    32281 gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca aagaaatcga
    32341 gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa tgaattaaaa
    32401 gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt aaaaacaagt
    32461 gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga tgagttagtt
    32521 gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa agcaagaaaa
    32581 atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga agatcttaag
    32641 aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca attagaacaa
    32701 gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt gactgacagc
    32761 catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga tactgatgta
    32821 aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca ggctattgat
    32881 atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact ttgttattct
    32941 taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa gatcctctac
    33001 ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt tattggtcac
    33061 agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca tgttcgtccc
    33121 ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta tggttgaatg
    33181 aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg taatttgcca
    33241 aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca aaaaagacaa
    33301 gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata tgccaatggt
    33361 aatatccatt taggacattc attgaataaa atcagtaaag acattatcat tcgttcaaaa
    33421 tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg attgccgatt
    33481 gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc agaatacttg
    33541 gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga tttcaaacgc
    33601 ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc ttatgaagct
    33661 gcacaagtac gcgtatttgg taaaatggct gaaaaaagct atatttacaa aggattgaaa
    33721 ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat tgaatataaa
    33781 gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa agatattctt
    33841 gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc agcaaaccaa
    33901 ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg ccgtaagttt
    33961 gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga aaatgtaaca
    34021 gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca tccattctat
    34081 gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg tactggatta
    34141 gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa atataaacta
    34201 tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc tggatttgaa
    34261 ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga aaaaggagct
    34321 ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac gaaaaagcct
    34381 gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg tcagaatatt
    34441 ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg tttgtataat
    34501 atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg agtaccactt
    34561 ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat cgaacatgtg
    34621 gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc gaaagaactt
    34681 cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa agaaaaagat
    34741 atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg tcagcgacca
    34801 gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg tggctggttc
    34861 aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa atcagttatt
    34921 tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt aggaaacatc
    34981 attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt atgggtatct
    35041 agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca agtggcagaa
    35101 gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga agatttcgat
    35161 cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata tatgaccgtt
    35221 cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata taacttcatg
    35281 catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt ctacctggat
    35341 tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg ttgcatgcaa
    35401 actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat cattccgcat
    35461 acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca gttaagcgag
    35521 tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg gactgctttc
    35581 atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag caaattgatt
    35641 ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg tgaaatgtta
    35701 aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta ctttgaagtt
    35761 atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt tgctattaca
    35821 gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa agatgtagga
    35881 gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga agacttctat
    35941 cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg tgtgacatgt
    36001 tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac gataccagtt
    36061 atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag ctgctttagg
    36121 tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc aagcttttcg
    36181 aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga agttcgttcc
    36241 atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat tttcaaccat
    36301 ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt gccctacttc
    36361 tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg ttagaacatt
    36421 aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata cattaacagg
    36481 aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt tgcctgtacg
    36541 tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc ctgcgacgaa
    36601 tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct tgaattgtac
    36661 accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca cttcttcttc
    36721 gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt ctttttcgga
    36781 aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat gattttgcac
    36841 catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta caccgaggtt
    36901 ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag cctcaaaaat
    36961 attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta aataatgatc
    37021 aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca attcaaaagc
    37081 agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc cattttttgt
    37141 cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag gtgccatagc
    37201 caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt cagacaattc
    37261 ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt aaaaatggct
    37321 tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt ctttgacaac
    37381 ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag caaaatgctc
    37441 gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac gctgggctag
    37501 gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca tgtggataca
    37561 ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac ggggattttc
    37621 tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac gatctcatat
    37681 tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt ttcatcaagc
    37741 actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg tcggcgttct
    37801 tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg ttcaggatga
    37861 ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc caatacttca
    37921 gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa aacttcccat
    37981 aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt caattgtaca
    38041 ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat catttcactg
    38101 acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac tttcgttgta
    38161 tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt catattcgat
    38221 catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg ttaattgact
    38281 atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa acgaaagatg
    38341 atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg agggatctat
    38401 ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt ttgttcggga
    38461 taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata ttcatctatt
    38521 tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc agatgtaatc
    38581 tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata ctttgagagt
    38641 ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga gagaacggta
    38701 atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct ttatgagaaa
    38761 ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa agctaaaatt
    38821 tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat aggatacata
    38881 gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt tttttattga
    38941 ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa ttattgcgca
    39001 tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag tggctattgt
    39061 atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa aaaagaaggg
    39121 tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg gtattttaga
    39181 cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc gtgaagtaac
    39241 ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa aagcatttgt
    39301 tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg atgcgcccac
    39361 actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg gttatgccgc
    39421 ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc atgaagaaat
    39481 gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc aaatcctcgc
    39541 ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc catttgatgt
    39601 agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga tcaacttagg
    39661 tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa gcacaaaaaa
    39721 atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat ttggttctca
    39781 ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata ttactcatat
    39841 ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta agcaagcaat
    39901 tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa aagaaatgga
    39961 tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag caagaaatat
    40021 ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg agataatcgg
    40081 tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag atggagaaga
    40141 accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag gtgtttacct
    40201 tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt tcccaagaaa
    40261 gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga attttgatgt
    40321 atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt attttgactc
    40381 aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc atgatcgcgc
    40441 gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg cagatgaagc
    40501 agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat tgttgtatga
    40561 agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa gaaaaaatgg
    40621 cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt tgtacaagta
    40681 tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc ttctcttatt
    40741 caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt ttctcctaca
    40801 ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga accaggtgaa
    40861 gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt agcagatgtt
    40921 gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg cggagccaac
    40981 gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg tacaccagga
    41041 agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa aacaatggta
    41101 tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga tcaaattctt
    41161 catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga tcgttccctt
    41221 gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac agcagaggac
    41281 gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa gaaagatgac
    41341 tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt taatcaactg
    41401 actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt aggaagtctg
    41461 gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa aaagaaaatg
    41521 gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac aggtcttcct
    41581 tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg ttcgggaaga
    41641 actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa taatctaaga
    41701 gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt tttatacgga
    41761 ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt aaataaaaag
    41821 tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga aaaaataaga
    41881 aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg caaataagcg
    41941 cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg aggggttatg
    42001 tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa ggattgacag
    42061 caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga tatttaagtg
    42121 aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt tactctttgc
    42181 caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa gtgacttttg
    42241 aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca aaagctgcga
    42301 ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt acagggaagt
    42361 cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt tctgcagatg
    42421 cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg ctctttggac
    42481 acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt cccggtctaa
    42541 tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta ccgccggaag
    42601 ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt ggagaaagtg
    42661 cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct tcagaaagtt
    42721 tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat cttgcttcac
    42781 gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa gcgaatcgtt
    42841 tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt tatgatgcgg
    42901 aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa tcttttttac
    42961 attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt tcccttcaag
    43021 tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga ttcatggaca
    43081 gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg tcacaagatc
    43141 cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct ctttccgaaa
    43201 ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat gcttatttcc
    43261 agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg ccagatgaaa
    43321 tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac cgtatttata
    43381 atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat cgtgtgagag
    43441 aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt aagagggaat
    43501 tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt gagattcctt
    43561 ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa agtgagcctg
    43621 ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact gcttcaagca
    43681 tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt aatatgccac
    43741 tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa aactatcaag
    43801 agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac accttcagtg
    43861 atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca agtaccatga
    43921 ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat atttatcaaa
    43981 atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa aagtctgaaa
    44041 ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga gtagctgcca
    44101 aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg atccagatgc
    44161 agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa gaatatgaga
    44221 tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt ttttcagcct
    44281 atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat gatgagatcc
    44341 cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt tctattgatg
    44401 aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg tcgattatcg
    44461 tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta gacggattga
    44521 tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa atttatcgtt
    44581 tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat caggtaaaaa
    44641 ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa ataaaaaaat
    44701 gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat aaaaaagagc
    44761 taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc ttgcgttata
    44821 taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat catgttagtt
    44881 tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc agcagaagaa
    44941 aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa taatttagaa
    45001 tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa agctcaattt
    45061 gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca agactatggc
    45121 atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata aatcaagtat
    45181 gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc atggggttaa
    45241 tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct gctaaaatcg
    45301 gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt atacaagcgc
    45361 atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg caagtgacat
    45421 tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact gatttagcaa
    45481 aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt cttttttgaa
    45541 cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa ctctctttat
    45601 ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga tggagaaata
    45661 catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt tgcgtgatgc
    45721 ttttatcggc acactgcccg caacaatggc gggatcagtc gctgtaatga tcaatgcaat
    45781 tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag cagcagacaa
    45841 aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct ggaacggtac
    45901 actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg cccgagctta
    45961 tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc aaggtatttc
    46021 ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg tcgcagatgc
    46081 aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag ttggattatg
    46141 gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta tgatcatggg
    46201 agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga tcaaaatgcc
    46261 tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag cgacgatcgc
    46321 tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg ggcaactgat
    46381 cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc aagggttggg
    46441 agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta ttcatggacc
    46501 aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa tcaatattga
    46561 tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag atgcaatcga
    46621 agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat ggtttggcgg
    46681 atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac gtgcagatta
    46741 tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg aaccagtgat
    46801 gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga ttgctccagt
    46861 agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc cggtatcgca
    46921 gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag gagcggattg
    46981 gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt gggcaccatt
    47041 tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag atgaacgtga
    47101 ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga actgaaatga
    47161 ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa ggatacaatg
    47221 aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc tttgaaacaa
    47281 gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa gcacagcctt
    47341 ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa aaagatagga
    47401 tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg atcgtgttgt
    47461 atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca ggtctttttc
    47521 taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa gcaaaggaga
    47581 aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt tttacgataa
    47641 agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag taactagaaa
    47701 acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg ctcgcataaa
    47761 aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa cgaagaatga
    47821 attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg ttcattacac
    47881 agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt taggaacaat
    47941 cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg aagcaacgta
    48001 ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc tagaaggaat
    48061 ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt aggaaggggc
    48121 agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga aatgtttgat
    48181 ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg ttttcgtaca
    48241 cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct aacaagtcaa
    48301 gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat ggccggcatt
    48361 ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca gatcatggaa
    48421 aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc gccattctcc
    48481 tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag ttatcaacta
    48541 aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg tatggatttt
    48601 gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa ccaacgagct
    48661 ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg caattaccag
    48721 tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta tcagcttgca
    48781 acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt ttgcagcctt
    48841 gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc atgcggctac
    48901 ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat gatcacacct
    48961 tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc atttaaaaag
    49021 agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg gaatgaagga
    49081 atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga acaaacaaaa
    49141 ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg gtatcgtaaa
    49201 gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga agaaacgtta
    49261 agctcaatct tgccacagca acagccaatt cttaatgact atttttgata aaaaacgggt
    49321 gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa ggaaaatgtt
    49381 aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt ttagggtttt
    49441 cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt ttagatgaat
    49501 ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag gacctcccta
    49561 cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa cgttttgcct
    49621 aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa caagctacaa
    49681 ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct cctaagcgat
    49741 aggtcgctgg ttcaattcca gtcaggaacg tacaaataac ccctgtatcc tttgcggtac
    49801 agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt
    49861 cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg tatgtcttag
    49921 tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag aaaaacttac
    49981 taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac gatttcgatt
    50041 ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc ggtataactt
    50101 gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta tttttagtaa
    50161 tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa aaaacggtgg
    50221 ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt ctatgcttta
    50281 tattattttt tgcatgtgtg cgaaagtata ctcaagccca atcgccattt cggaaaatcg
    50341 gtactgtaga gccatcttca cggattccgt cgatatccat cttgtcagat cctaccatga
    50401 aatctacgtg ggtttgactt ctattcaatc cagcttctgc taattcttca tcggacattt
    50461 ctgtaccgcc tttcacactg aaggcataag cagaacctag tgctaagtga ttcgatgcgt
    50521 tttcatcaaa taatgtattg aaaaagatga tgccagattg agaaataggt gaagggtcag
    50581 gtacaagtgc gacttctcct aaacgacgcg caccttcatc tgtatcaagc agttttgcta
    50641 agacatcttc tccttgttcg gcagaaaaat caacaacttt tccatcttta aaagtaaatt
    50701 tcatacccga gatgatggtg ccagcataac tcagtggctt tgtacttgaa atgtaaccat
    50761 ctactcgatg actgtcaggt gctgtgaaca cttcttccgt cggcatattt gccatgaatt
    50821 tttctccacg agcattatag cttccagcgc cttcccaaag atggttttta ggcaatccga
    50881 taatgatatc tgtaccggga gcagtatagt gaagggcaga aaattgttct cgattcaatt
    50941 cttctgcttt ttttgctaat ttttcatcat gcttcttcca tgctaagaca gggtcttctt
    51001 catagacacg agtcgttttg aaaatctgat cccataatgc ctcaacttgt ttttcttctg
    51061 gcagttctgg gaagactttt gctgcccatt gtttaccagc cgcagccacg acagtccaac
    51121 tgactttatt tgcttgagtt gccttgcgta aattcattag tgctttgcca ttcgctgatt
    51181 gataagacgc tactcgatgg ctatctacac cagcaaatgc atcgggatcg gcagaaacaa
    51241 cgctgatcct gcttgctcct ttttcaagcc attcatccgc ttgatcgatt ttagattgtg
    51301 gtacattctc aatacgatcc gttgcagcat gtaaaagaaa ttctctttgg atctggtcgt
    51361 cagtccattg aacgataacc tcagcggctc ctagtttata agcttcttgt gtgatcaatc
    51421 gagcaagggg tgcctgttcc acgctgatct gtaaaacaac tgtgtgtcct ttttctgtgg
    51481 ctacgccagt ttctgcaatt aagcgtgcat atttttttag aagttcatta aaatcagata
    51541 acataataaa aattcctcct caccattttt tctggtaact attagcatga taccatttaa
    51601 ttatgattca aacaatgagg ctttaaataa aaaaaggagg ttattttttt aaggttaagt
    51661 ttcatgatat atgttactac ttgctatttc agcttgtgaa gaaaatcaaa aactaatttt
    51721 gcatatcaaa aaaacatgtt acaatgaaat taatcaatgg tggaagcgtt ataaaaaaat
    51781 taaggagatt aaaaaatatg gcaagaaaaa agacgattac tagagaacag attttggcag
    51841 ctgcttatga agtagctgcc accgatggat ttacacgatt tactgcgaga aacatcgcga
    51901 acaaaatgaa ttgttccacg caaccgattt atcttgagtt taaaaatatg gatgacttga
    51961 gacaagctct ttttgagcaa atctatgatc atttacaata tgaagtgttc actgttgagc
    52021 atacagggaa tacgattatc gatcttgctt ttaattatat ccattttgca agaagagaga
    52081 agaagttgta tcgttcactg tacttagaag aatatggtgg aggaaaagag atgcagaatt
    52141 tctcttatac ttactttgtg aatacagtga aacgcgatcc agcttatgtg aatctttcag
    52201 aagagcagat cgaatctttg tataatggga catggatcat agctactggg atcgcagctt
    52261 tgatgtcttc aagcatcatc catccaactg atgaacagat tgaaaagatg atccaagaca
    52321 gtatagatgc tatcttgaaa ttcaaagaac caatcgaaat cgattgaata tactcaaaag
    52381 ttccacaaat cagtaggtaa caagagatgt gtcaaaataa ggaaaagttc gtttttctta
    52441 cagcgtatgc atttttaact cccgaagaac tcttccatga atgcaatgaa taaaagagcc
    52501 atacagattg aataaatctg gatggctctt ttattgcaag tatattaaat ttttgcgact
    52561 agttctttag cgaaagcttc taaattttcg atatcttctt cttcagcatt cagatccact
    52621 ttaacatttt cggctccttt tgatgcgcct gtttttgcaa aagcagcatc gaaatcatcc
    52681 accgatttgc agaattcatc gtaaaaagta tcaccagatc cgcagacacc gtaaattttt
    52741 cccaatagat ccaattcttg caagtcttca taaaagtcta cgatctcatc aggcagatcg
    52801 ccatcatcgt atgtataagt agctactacg caaatatctg cctcctcgaa ttcatctgca
    52861 tctacttggg tacattcgtt gatttcgact tcgatatcca gattttcaaa tgcttctgca
    52921 acaatatcgg cgatttcttc agtgtttcct gtcatgctag catatacgat ttttgctaaa
    52981 gccatgatgt gcctcctcgt ttataattat ttaacaatta tgaattatac caaaacatca
    53041 ttgaaaagaa aataaaacct aaagacacct ggggaatgtc atttggctaa atgtatgccg
    53101 ctatgttaaa atgagaggga tttgtaaaaa ggagacgaaa atatgattac tttaaaatca
    53161 gcacgtgaaa ttgaaatgat ggcagaatct ggagctttat tagcagatgt acataaaaat
    53221 ctgcgtgatt ttataaaacc aggaatcaca agttgggata ttgaggtatt tgtcagaaac
    53281 tatatcgaga gtcatggcgg aattgctgcg caaattggct ttgaagggta tgaatatgcg
    53341 acttgtatca gtataaatga tgaaatttgt catggattcc ctcgtaaaaa gcctttgaaa
    53401 aacggtgatt tggtaaaagt cgatatgtgt atcgatttga aaggcggcat ttctgattct
    53461 tgttgggcct atgtagttgg agaatctaca cctgagatcg accgattgat ggaagtaacg
    53521 aagaaagctt tgtacatcgg tattgagcaa gcacaagtag ggaatcggat tggtgacatc
    53581 ggtcatgcta tccaaactta tgtagaaggc gaaaatctag cgatcgttcg tgattttatt
    53641 ggtcatggcg ttggtcctac gattcatgaa gaaccagcag ttcctcatta cggagaagct
    53701 ggaaaagggt tgcggttgaa agaaggcatg gttatcacga tcgaacctat ggttaatacc
    53761 ggaacttgga aaatgaaaat ggatcctaac ggctggactg cgtatacaag agacggcgga
    53821 ctttcttgcc aatatgaaca tacattggct attactaaag atggtccaag aattttgaca
    53881 tcacaaggag aagaaggcac ttattgatct atccagtcaa taggtacggg agaggtacat
    53941 gaaagtttgg aacaaaataa aacaaaatga aaatctgatg cgctttattg agacgactca
    54001 aaagcgcatc actgattcag aaatgagtac gacctctgtc gtcgttgctt attatttact
    54061 cttgtcttta tttccattga ttattgcttt tggtaatatt ttaccgtttc tccacatcga
    54121 tcaagagact gtactgacct atttacgaga agtgattcct gagaccgttt accagtttat
    54181 cggtcctgca atcaaagacc ttttgacaca aagttcagga ggcttgttat ccgtttctgc
    54241 attagcggct ttatggtctg caagtcaaag tatcaatgct ttgcaaattg cgatgaataa
    54301 agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc cgtttcttct cactaattgt
    54361 cattgtcttg ttcatgattg ctattagtgg ggtaactatc gttcttgggt tgggacagct
    54421 gattttggat gctattcagc caatcgtaca aatacccgtg aactttatcg atcagttcca
    54481 aactttgaag tggccaatca cattggtagc tttatttgtc atcatgttct tgatttattt
    54541 aattgtccct aatgctcagt tgaagttgaa gacgatcatt ccgggcacga tcttcgctac
    54601 tgtcggctgg atgctgttat cacaagtgtt tagtatctat gctaggtatt ttgcaacgag
    54661 gatcagcggt tatcagatca taggaagttt tattgtactc atgctttggc ttaattttgc
    54721 tgctacgatc atcattttgg gcggaatcat caatgcagtt gttcaagaat acgtgacggg
    54781 tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg ttcaataaat tgaaaactaa
    54841 attttctcat aaaaaaaagt agaaacaaga gttcttattg taaatttata tcagtaattt
    54901 ataataatta tgactcttaa ttaaagtagg tgcccatatg cttgtttctt ttagtatggt
    54961 tataagattt tttctgacaa ttttattatc actgatatta acacctgtat tcaaaatcat
    55021 ttcagtacaa acaggtatgg tcgacaaacc aaatgaaaga agaataaata aagttccaat
    55081 gccttcagcg ggcggactgc ctatctttat ttcatttgtt atatcgactt tattcttatt
    55141 ccgtaaaatt atccctcaat cgtatattgt accgatcttg attgcttctt tagtaatcat
    55201 agtcacagga gtgctcgatg ataaatatga gctgacacct aaacaaaaaa gtatagggat
    55261 cttgattgct tctcttatta tttattttgt agcagatata cgtattgatt ccttcacgct
    55321 tccttttatt ggatatatcc aactaggctg gcttagtttt cccgtaacga ttttttggat
    55381 cttcggtatc acgaatgcgg tcaatctgat tgacggtttg gatgggctgg cggcagggat
    55441 ttcattgatc ggtttaatta cgataggaat cattggatat tttttcctcc atgcttctac
    55501 agtgtatatt ccagttgtta ttttctgtct tgtagcaagt attatcggtt tttttcctta
    55561 caatttttat ccggcaaaga tttatttagg cgatacagga gctctattcc tagggtttat
    55621 gatggccgtc ctttcactac aagggctgaa gaacgtgact tttgtttcat cgatttcttt
    55681 gctgatcgtc atgggagtgc cggtaacaga cacgtttttt gctatcatta ggagaaaagc
    55741 aaaccgtgtt tctttttcta cagcagataa aaaacatctt catcaccgac taatcagttt
    55801 aggctttact cataaaggtg ctgtcctgac gatttatgcg atggctttga tgttttcttt
    55861 cacagctatg gtcatgaatt atactggaag aatcggaacg atcatattga ttattgccat
    55921 gttgtttgct gcaattttat tatttgaatt gattggtctg atcaatgaga aacatcagtt
    55981 tattttaaaa acattgcgat ttttagggaa taaagagtac cgtacgcaag taaaacagaa
    56041 atatttgaag aaatggaacc gtcatataga tgaataacaa attaaaccag ccgatcctta
    56101 ttagtattgt gacggcaaac agtgataaaa tttttcggac actagataat tttatttcaa
    56161 ctgtaaaaga tgaaacagca gtaaaaatcc gaatttttga caacaattca cttcccgagt
    56221 acaaaaaaag actaacagag tataggaaat atccctacat cgatatctat tttcatgatg
    56281 aaaattccgg atttggttat ggacataatc acaatctgat agctagcgat tctcgctatg
    56341 gcattatttg taacccagat attttagtgg atgaagagac agttacagca ttggtcttgt
    56401 tattgaaaga acatccggaa tgtgcgatgt tagcacctaa gattcttaat gaagatggaa
    56461 caactcagca cctcatccgt gagcagctgg ctgtttttga ttacgttttg cgctttgtgc
    56521 ctttcaaatg ggtcaaaaaa aagtttgaca aacgactggc tgtgtttgaa tgccgaggat
    56581 tgcctactga tgctaatagt tatgtacgga tgatctcagg tagttttatg gtagctgata
    56641 ttgataaatt taaagagata aaggggtttg atgagcgttt cttcatgtac tttgaagaca
    56701 acgacttgtg tttgagtttt gaaaaagcag gttacaagtt attgtacaca cccttttatg
    56761 cagttatcca tttttacggg aagggtgcgc atcgaaatgc aaaactgttt cgcgtgtttt
    56821 tgacttctat gatgaaattt ttcaataagt gggggtggcg ttttttctga tgaaaccaga
    56881 aatattagta gtcgttgtgc tttatcagca actattcagt caatctccag cttatggacc
    56941 attaagcaaa gcacttagcg aaaagcaaat tcagcttatt atttacgata atagtcctca
    57001 aaggcagcaa aatgagctgt ttgaaaaaga aaatatcgtt tatcatcatg atccgcaaaa
    57061 tcctggttta gcggtggctt ataattttgc gttgtctcaa gcagatgaag gaacacgttg
    57121 tctcgtcact ctggatcagg atacgaggct agtcgacgat tattttgaac tattgcgtaa
    57181 agtaaccttt acagatgaat gtgttgctgc agttccgatg attttttctg ggagccgaca
    57241 agtttctccc ctatattcag atcgttatat caatcgtaca gctcaagcag tagaagttga
    57301 aggcccaaca gctgaacgaa tcatggcaat caattcaggt gttgcctggt ctgtcaaatt
    57361 tctaaaaaag attggcgggt ttaatcctgc tttttcttta gattttttgg atcattggct
    57421 gttttggaaa gtggatcagt tggaaaaaac tgtggagatc cttcctgtac gaatggaaca
    57481 tgatctttcg gttttagact atgaaaaagt ttcaagtaat cgataccgtt ctattttaca
    57541 agcagaaaat cgattttatc atgaatatga tcaaaggcat ttatccagtc accgacgaca
    57601 attattgtta agaacagcta aacagttctt gaccgtcaaa aaccggcata tctggcggat
    57661 gactttgcgc tcatatgtta agaattggaa ggtgtgaatt cgtgcgttcg gtttgtatag
    57721 ctacctttaa tggaggcaga tatttaagag agcagttaga tagtatttta tctcagcttt
    57781 cagcagaaga tgaagtagtc atttctgatg atgggtcagt agatgataca tgggatatat
    57841 taactgaata cgcagaaaaa gataagcgaa tccagctttt ttcggggcct cgtcaaggat
    57901 tgatcgctaa ttttggttat gcgatccagc agacgaaagg agatctaatc tttttggcag
    57961 accaagatga tgtttggttg gagacaaaag tgaaaaagat cactgactat tttgagcaga
    58021 atccggatca gctagtagta gtttctgatt taatcattgt ggacagcgag ctgcaagaga
    58081 tccatgattc gtatttttcg tttcgaaatt ccaaagaagg atggcttaat aatgtgataa
    58141 gaagtcacta tattggtgct ggtatggctt tccggtcttc tttaaaaccc gatattttgc
    58201 cgatcccgtc agaagtgccg atgcatgata tgtggattgg tttgctggga ggaaagaaga
    58261 ctgggttcct aagagaaccg ttaactctgt accgaagaca tgatttaaac gcgagtgaga
    58321 ttgaaacaca ttctaaatta cggcaaaaaa tttcttggcg tgttcatctg attacggcgt
    58381 tgatcaaacg aaaatggttt aaacgttgat aaaagatgtt taaatcctta atatttccat
    58441 gacaaatata acaattgatg agataataac aaagtgaaat ttgaaatcaa aaaggagagc
    58501 agatatccat gaagggaatc attcttgctg ggggcagcgg aacacgtctg taccctttaa
    58561 caaaggcaac atcgaaacaa ttgatgccga tttatgacaa accaatgatt tattatccaa
    58621 tgtctactct gatgttggcg ggaatcaaag aaattctgat tatttcaacg ccacaagata
    58681 caccacgttt caaggaatta tttggaaacg gaaatgattt aggactgcag atcgaatatg
    58741 cagtacaaga aagtccagat ggattggcac aagcgtttat cattggagaa gaatttattg
    58801 gagatgatag tgtctgcttg gtattagggg acaatattta ctatggtggc ggtctgtcta
    58861 aaatgttgca acgagcagct tcaaaagata caggcgcgac agtatttggc tatcatgtca
    58921 atgatccaga acgctttggt gtagtagaat ttgatgagaa tatgcaagct ttgtcgattg
    58981 aagaaaagcc tgctcagcca aaatcaaatt acgctgtaac tggcctttat ttttatgaca
    59041 atgaggtaat ttctatcgct aaaggtatca aaccttcaga acgtggagaa ctagagatta
    59101 cggatgtcaa taaagcatat ttggataaag gaaaactatc cgtagaactt atgggacgcg
    59161 gatttgcttg gcttgataca ggaacacatg aatcattgct tgaagcatct acctttatcg
    59221 aaacaattga aaaacgtcag aacttaaaag tggcttgttt ggaagaaatt gcttaccgta
    59281 tgggctacat cgataaggaa caattgctgg cccttgcaca gccattgaaa aaaaatcaat
    59341 acggtcaata tttattgaat ttggccgctg aataaggagt ccttacagat gaaagtaaca
    59401 gatacaaaat taacagatgt caaaatcgta gaaatggacg tctttggcga tcatcgcggt
    59461 ttcttcacag aaagttattc aaaagcaaaa tttgcagaac atggattgaa atatgacttt
    59521 gttcaagaca accattcact atcagctgaa gctggtgtcc ttcgggggct tcatttccaa
    59581 aaaggagaag ctgcacagac aaaattgatt cgggtagtca ctggagcagt tttagatgta
    59641 gtagtggata tccgcaaagg tagcccgact tatggtcaat gggacggatt tatcttatct
    59701 gaacataatc atcgacagtt gcttgtacct aaaggatatg cccatggatt cgttactttg
    59761 acaccaaatg tgaacttcct ttataagtgt gataattact ataatgcaga agcagatggg
    59821 ggcatcgcat ttgatgatcc agatttagcg attgattggc caattgatcc atcaagagcg
    59881 attatgtcgg aaaaagatca ccgtcatccg acattaaaag aatttgaagc agaaaatcca
    59941 tttgtttacg gagaaatcta agaattaagg agaaattttc ttatgaaaaa catcattgtt
    60001 accggaggtg ccggattcat cggctctaat ttcgttcatt acgtggtaaa caatcatcca
    60061 gaagtgcatg tcactgtttt ggataaactg acttatgcag gaaacaaaga gaacttggcc
    60121 ggacttccta gcgatcgcgt agaattggtc gtaggagatg ttgcagatgc agaattagtg
    60181 gaccgtctag taaaagaagc ggacgcagtt gttcattacg cggcagaatc ccataacgat
    60241 aattcattaa aagatccttt cccatttgta caaacgaata tcattggtac gtatacactt
    60301 ttagaagctt gccgcaagta tgatgtacgc tatcatcacg tatctactga cgaggtttac
    60361 ggcgatcttc cattacgtga agacctgcca ggacatgggg aaggcgaagg agaaaaattc
    60421 actgcggaaa caccatataa tccttcaagt ccatattctt ctacaaaagc tggttcagat
    60481 ctgttagtaa aagcatgggt gcgttctttt ggcttacgtg caaccatttc aaactgttca
    60541 aacaactatg gtccttatca gcatattgaa aaatttattc ctcgccaagt aacgagtatc
    60601 ttaagcggta ttcgtccaaa attatatggc gaaggaaaga atgttcgtga ctggatccat
    60661 acgaatgatc attcttcagc agtgtggctg attttgacga aaggcagaat tggcgaaacc
    60721 tatctgatcg gtgcagacgg tgaagaagat aataagaccg tgatggaatt gatcttagaa
    60781 atgatgggcc aaccaaaaga cgcatacgac catgtaaacg accgcgcagg gcacgatttg
    60841 cgttatgcaa tcgattcgac aaaattgcga gaagagctag gttggcagcc tgagtttacg
    60901 aacttccgtg atgggttggc tgacacgatc aaatggtacg aagaacatga agactggtgg
    60961 aaaaaagaaa aagaggccgt tgaagcagca tatgccaaaa atggacaata atacagagga
    61021 aatttcactg tattaatgga aaaacacatg tgaggcaggc gctcgcctgt ctctttttta
    61081 aaataaaaat caaggagcgg ataaccatat gtttttatta actggaggaa atggacaatt
    61141 aggtacagaa ttgcgccatt tattagatga aaaaaatctg ccatatgttt caacagatgc
    61201 aaaagacttg gatatcacag atgctgagaa aacaatggct tatattacag aattaaaacc
    61261 cgatgttatt ttccattgcg cagcttacac agcagtagat aaagcagaag aagaagcaaa
    61321 agaattagat gagaaaatca atgtcgatgg cacaagaaat gtagcacttg cagcaaaagc
    61381 tgtgaatgca caattcgtct atatcagtac agactatgtt ttcgatggaa aaaacaaaaa
    61441 tgtagaatac gaagtagacg accaaacgaa tccactaaat gaatatggtc gtacgaaatt
    61501 attaggcgaa aaagcagtag aagagatttt agaagactat tatatcatac gaacttcttg
    61561 ggttttcggg atctatggac acaatttcgt cttcacgatg caacgcctag cggaaacccg
    61621 cgacaaactg actgtagtaa atgaccagtt tgggcgacca acttggacac gtactttggc
    61681 agaatttatg gtatatgtca tcgaagaaaa agcaccattt ggtatttatc atttgtcgaa
    61741 cgaaaatagc tgttcttggt atgagttcgc aaaagaaata ttgaaagata cagatgtaga
    61801 agttgcacct gttacgtcag aagaatttcc gcaaaaagca acacgtccgc agtattcagt
    61861 catgagcctg aaaaagacag aagcgttagg ttttgtcata ccaacttggc aagaagcact
    61921 tgcgcaaatg ctggaagctg ccaaaaaaat gaataagtaa tttccttttt atcaaataga
    61981 atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa aagccaaaag
    62041 cgaatcaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc tatcttattg
    62101 gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca cttggtgttt
    62161 gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt tttggacaga
    62221 atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc tcacaagctg
    62281 aagaggactt ttcgttggtg aacgaggatt attcattaaa cggacaaaac atgatcattg
    62341 cgtataattc tcctgtaaag gatattacgg ttatcggaaa accattcaac tggggattct
    62401 ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta gtcgtcatgg
    62461 tcttgcttgg attcgaagta ctgatgatat taacgaagag gaaaaaagct ctatcgctaa
    62521 tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg caacatgtgg
    62581 gcgatttgat tttctttact ttgggactga tgattgcttt ttatcattat ttctacaatc
    62641 atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc ggtattgggt
    62701 ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg attttttatt
    62761 ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg gatgtagtgt
    62821 tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg ctaacgtcaa
    62881 aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta agtactggag
    62941 gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt ccttttgaag
    63001 atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt ccaactgtat
    63061 tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag ttactgggac
    63121 gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg ttgccaaaat
    63181 ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc cttactttta
    63241 gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga gaagatgtta
    63301 ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat ttctatgcga
    63361 ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca gttgctgtgt
    63421 tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt tacctcgctt
    63481 ttgtcgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa ggtacaggag
    63541 cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa gatcctggta
    63601 aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga gtgaaatcct
    63661 tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat ccgaaagaag
    63721 aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct aaggaagata
    63781 caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt gaagacgcag
    63841 aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac aaccaattat
    63901 atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg atttttaaag
    63961 tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat cagctagtcg
    64021 tacagtaata ggctagctga gaataatcct gaaatattag tagaaaggag agtgaagata
    64081 attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt gcttttgttg
    64141 ctgacgctag tcttttttgt ttggacagca ggggtaggtt ttccagcaac tttgtttata
    64201 tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat tttaaatgtt
    64261 tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct tttcattttc
    64321 ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact gtttcctttg
    64381 attattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga tcgcggtatt
    64441 tatctgattg tctttacggt cttcctcgga atcacgttat atcgtgtatt tacagagatc
    64501 caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga tatttggatc
    64561 aatacgaata caatcggaag ttctttgatg actcttgctt tattgatcag tggatttgcc
    64621 agttcttttg aacgatggta tgttcgaatc atcggtggga tagcagtcgt tgcagccttt
    64681 ttaggtattt gggtctgccg atctagaggt gcattagtcg cgttgattgt ttttgcgata
    64741 ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat tgcctacacg
    64801 gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga aaaagtcaat
    64861 ttgtttaccg gtcgagaaga tatttggcat aaattctatc agacgctagg agaaaaatct
    64921 gaacaagttt tactaggaat gaaaacattt atcttccagc gaggaaacca atttttagga
    64981 aaccacaata gttataattc tattttgaat atctatggat tgataggatt tgggattgct
    65041 gctttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt atccaacggg
    65101 cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat ggaggataca
    65161 ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc cgttcatcga
    65221 tatgatgaac cagaagttac ggaggaattc tatgttatgg aagaggcata tgacgatatg
    65281 acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc caggatccaa
    65341 cgctatcatt gataggcaca aaatacgaga gaggctgtga caaccgtcgc agcctttttt
    65401 ggtttattgc aattgtctag catcttctgt aaaatagaaa cgttagagaa aatcttgtat
    65461 catagaaaat taagaaagaa ataagaacgc agctcggtac caaaaatatg gtaaaatggc
    65521 acttgactgt aattcactga aaatcgcttg gaggaacaca aatgtttaaa gatttgtatt
    65581 tatttatgaa ggacatttat cagaacagaa aattactgct acagttttcg atcaatgact
    65641 tcaaatcaag atatgcagga tcgtttttag gaatcttatg ggcgtttatc aatccgctat
    65701 ttactgtatt gatttattgg ctggtatttg gtgttggact gaaagcacaa atgatcgatg
    65761 caaaataccc atttatcgta tatttagtta ccgggatgat tccctggttc ttcttctcag
    65821 atgcatttgc atcaactacg gttgtttttc gggaatatac ttatttagta aagaaagtcg
    65881 tattcaatat tcgtatcttg cccacgacga aaatcctatc caatctgtac acccatttat
    65941 tcttcattct gattggcata gctgtcgcgc ttataaatgg tatatatcct tcagcaatgt
    66001 ctttacagct gatttattat ttattttgca tgtgtgcttt cttgacagga ttgacttggc
    66061 tgacggcatc gatccaacca tttttacctg atatcatgca attaattacg atcgtgctgc
    66121 agttgatcat gtggacatta ccgatattat ggagccctaa tcagtttgat tcaggtcttg
    66181 taagcatatt gaaattaaat ccgctgtact atgttgttca aggttatcgt gagtcatttt
    66241 taagtgaagc ttggttctgg gagcatggaa catatacttt gtatttctgg gttttcacgt
    66301 tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat ttttcagatg
    66361 tgctgtaagg agaatagcta tgacagaata tgcaattaga ctaaaagata tcacaaaatc
    66421 ttataatatg tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc cttttaagaa
    66481 atcctatcat gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa aaaaaggtga
    66541 gatgatcggt ttcgtgggag aaaacggatc gggaaaatct acaatgttga aaatcattac
    66601 gggagtactg acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct cagctttgct
    66661 agaactagga tcaggattca atcctgagta ttctggctat gaaaatatct atttaaatgg
    66721 aatggttcta ggattttcaa gagaagaagt cgatgaaatg gtcgatgata tcattgaatt
    66781 tgctgacatc ggcgaccata tttatcaacc tgtaaaaacc tattcaagtg ggatgttcgt
    66841 tcgtctagct tttgctgtag cgatcaatgt gaatcctgat attttgattg tggatgaagc
    66901 attggcagta ggagatcttg aatttcaact aaaatgtatg gaaaaattta cagaaatcaa
    66961 gaactcggga aagacgattt tattcgtttc tcatgatatt agttcgattc gccgtttttg
    67021 tgatcgttcc tattggttga aaaatggaaa agttgtcgaa tatggtgata cgatggaagt
    67081 aacgacgaat tacgaaaact ttttgaagaa aaaatcagtt aaaacagtag atcgaaataa
    67141 aaaccaagta gaacgcttcg cagctccaga tatcgtagac gtgacgaaag ccgaattgtt
    67201 ggataaagat aaaaatccag tggatatgct ggaacaagga gaaaaactat acgttaaagt
    67261 aacctatgaa gtaaaagacg atacaatcaa aaatccagtt ttaggaattg ctctgcgtac
    67321 agtcgacaat ttttatgtgt gcggactcaa tactctacta gatggtatca agatcccttg
    67381 gaaaaaaggt gagaatatct tttatctgga gtatgaaaag ttaggtttgt tagctggtga
    67441 atattatttt gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg tatacaagac
    67501 aaaatacatg aacttgtttg tcagcggttc ttatatcgga gaaggcatcg tggtcttaga
    67561 ccataaatgg gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg gacctagatg
    67621 aacaaagcag tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa gttttagagt
    67681 ttggtcctgg aaacggccga ttaacgaaac atttgatcgg agcaaaacaa tgtgaagtga
    67741 gcattgtcga gctagataag gaattgttcg attttgtcag cgagtttgcc caagatggat
    67801 tttatggaga tatcgaaagt tttgaatggg caaactatta tgctggacaa acatttgatt
    67861 acgttttgtt tgccgatgtt ttagaacatt tggtagatcc tggaaaaacg ttgaagaaag
    67921 ttagagaatt tttgaatgaa gaaggagaaa ttctaattac attccctaac cttgcgcata
    67981 attctgtcat gattgatttg ttcaataatc aattgccatg ggcttcttat ggtttgctag
    68041 atgaaaccca taattctttt tatactcatg atggcttcca aaaagttttt gaaaaagcag
    68101 gattatacat caatattgaa gattatttgt acttagctgt aggtgataca gaattaaaat
    68161 caacttatga agaattgccg gaagctgtac gttatgattt caaaatgcga ccatttggcg
    68221 aagtctacca atacttcttc tcattgatga aacatccagt agcacaaagt tccattgcgc
    68281 agccgcaaaa ttctaattat gtgaaagtat tagaagtaat ccagcaaacg aaacaagatg
    68341 aaataattca acaaatacca ttcaataatt tcacagggga aaatgagaca ctaagtttcc
    68401 atgtatcaga attagcagaa cgaatggtgt ttcgcttcgc gcagcaacct agctttatcg
    68461 aattcagcgc agaagctgct ggagaaaaac tcacatttat cgattcaaat gctgtagtca
    68521 aaacagtaaa tgattgttat ttgtttgatg gtaaagattt accagagttc tttttaacgg
    68581 atattgcagg gaaagaagtg actatccatt gtcactatcg ttttatcgga gaattgacac
    68641 caacaatgaa agaattgctg gaaacaatcc gtccaatggc tgaagttaca aagcagcttt
    68701 ctgagaaaaa tgatgaatta gaaagagaaa atcattatct tattgagaat aatacaagat
    68761 tagatcagac gttgaaaaca accataaatc gctattgcac gttgttggaa agtgatgaat
    68821 ggacaatcaa acatagatta gggcgacgta agaaagaaac atcgaaaaaa atccaagaaa
    68881 aagagctttc tctatgtatt gacgaaaaaa tctgggatgc agaaacaaag atattgaaaa
    68941 tcatcgggtg gggaatcgct aattcggatc gccaaccatt gtcctataag ttatcagccg
    69001 accaatcacc attttttgaa gcaattcctg ttttacgaga agaagtcaat caagcaaaac
    69061 agctagctga aggagcaaaa gcaggatttg aactgcggat cctttgcgag aaggaacgtt
    69121 cattcttagt tgaagcagtg gctcaaaatg gtcaatcatg gatcattgaa atgtaaataa
    69181 tgattaactt ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa ttgccgtatt
    69241 tatcgatcaa atcactcgtg aacgggccac tggcgatttg cttattgtgg gctgggcaat
    69301 tgatgaagtg accaaagaaa tccctacgat aaaggtagaa aaagaaaatg tgattgctga
    69361 agcaacacat gtcgtaagat tggatatcaa tcatttatat aatcttgatg ttaagaccca
    69421 aagcggtttt aaggtacgtt tatcagggaa aatgagagga aaagcaatct tagacttcca
    69481 aacagcaaga catcaaaatg gcatagctgt gaaattaaac ggtaaatatc cttatgatga
    69541 cggcattgaa tcttcatggg aaagaaaaaa aagattactg aaaaaaggga taaattatgc
    69601 aaggactcat ggcgtaaaaa aagcaatccg ccgggtcaaa ctggaattga aaccaggatc
    69661 gatcgactat gcagagtgga tcagtcgtca tgagaaacct gatctgaaag agcaaaggaa
    69721 agaagtacaa ggatttgcct accgcccatt gatttccata cttatgcctg tctataacgt
    69781 tgaaatcaaa tggctcgaaa aatgcattga ttcggtactt gatcaaacgt atgatcactg
    69841 ggaactatgt atcagtgatg atgcttctac tgatccagct atccgaaagt gtctagaatc
    69901 ctatcaagca aaagatgata gaattaaagt cgttttccga caagaaaatg gacacatcag
    69961 cctggcaact aattctgcat tggaaatggc agaaggagag tttatagcgc tcttagataa
    70021 tgatgacgaa ttacctccat ttgccttata tgaagtggca aaagtgctaa acgtacatcc
    70081 agaactggat ttgatctaca gtgacgaaga taaaatcgat gcagatggga atcgctttga
    70141 cccgcacttc aaagcagact ggtctcccga tacattgatg ggaaataatt atatctctca
    70201 cttaggcgta tatcggacaa gcattgttaa ggaacttggc ggtttccgta aaggatatga
    70261 aggctctcaa gattatgatt tggttcttcg tgtgacagaa caaattcctg cagaacacat
    70321 ttaccacatt gaccgtgtct tgtatcattg gcggacgatc ccgggatcaa ctgcaagcaa
    70381 tggagaagct aaaagctaca tctatgattc tggagtgaaa gcattgacag atgctctatc
    70441 tagaagaaat atcaagggaa gcgtacatcc tggcagaatt tctggttttt atgaaatcac
    70501 ttatgatgtt ttgcaagaag acttggtcag tgtgatcatt ccaactaaga atggctatga
    70561 agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg tcttatccaa attatgaaat
    70621 cattattgca gataatggaa gtacagatcc taaaatgcaa gaattatttg cagaatacaa
    70681 gcatcagctg aaagaccgat ttatcgtcga attaattgac attccattca attattcacg
    70741 tatcaacaat ctggcagcag aaaaagcaaa cggcaaatac tttttattct tgaataacga
    70801 tacagaagtg attgaacctg actggatgac agcgatggtt tcttatgcac agtttgatcg
    70861 aatcggctgt gtaggagcaa aactgtttta tccagatgat acaacgcagc atgcaggagt
    70921 attattagga atcggtggtg ttgcagggca tgcattgaat aactatgatc gaacacattg
    70981 tggttatttc ggccgattgg tcattgacgt caattactta gcagtcactg cagcttgtat
    71041 gatggtcaag gcagctgact tcaatgctgt taacgggttt gatgaaacat tagaagtagc
    71101 attcaacgat gttgacttgt gcttgaaagt ttatgaactg ggtcgttaca atgtctatgc
    71161 acatcaagca gaattgtacc atttcgaatc taaatcaaga ggttatgaag atactccaga
    71221 aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa gataaatggc cagcatatat
    71281 tgcgcatgat ccattttaca atgataattt aacaaaagaa ggaatcggcg acttttcgtt
    71341 gagaccagat taaactataa taaaaagctg ttatgttgtg attcgctcgc acataacggc
    71401 tttttatagt tttttgtcaa aaaaaggcag aaggtgccga ctcaaaaaaa taagtcgagg
    71461 ttgggacaga agtgtttaac tccgagaaat aagaagaaat tcacgaaaat tgcttttcaa
    71521 atttttgtga atttcggctt atttaagaag aagttgcttc tgctcccacc gtttattcgt
    71581 ttttaaagaa tgagacaaaa gtgtttctta cttttgtccc acgctcttat tcatgctcag
    71641 agcgccaata gatacaaaat catagccaaa gtttcacgaa ccctagctgt caaagaatcg
    71701 atattttatt ttaatatagg ctattttaaa ataaatggtt taaggtattc ctctacaaaa
    71761 gccactctat caattatcat gggtttaaaa taagaagtga tggagataac cagattgttc
    71821 ttagcgtcaa tataaagaac atttccacta ttaccaattg ctgaatatat ttcgttctca
    71881 gaatctaata cccaccataa atagccatat ctcatatttt gtaagtcttc taaagcactg
    71941 gttctaggtt tgaacatctc gtttatccac atgggtggta cgatttgttt tcctttatac
    72001 atccctttat ttaggcataa tacaccaatt tttgccagat cacttgccga taaacacaaa
    72061 ccatatcctg ccgtcccgat atttagtgga tcacaaaacc agacattggt tttaggagac
    72121 ttatccattg taaactgctt atgttcttca gctgtttcag cataataatt gacatggttt
    72181 ggtatcccta taggatcaaa tagatactta ttggcaaaat caacagttgt tagcttactt
    72241 gtctgataca acaagccagt taatatgtgt aaacagacag tttgatagtt gaattcgccg
    72301 gttatacctt ttcttcctcc caataaatct agtgatgttt ttgtccaatc ttcacttgaa
    72361 cagacttttg tccatggatc gcctttacat ttgtagggtg ctttcattgt tagcaagtgc
    72421 ttgattgtaa tagcgtaaat agttttctcc ccgcgtttca ctttatactc tggaaaataa
    72481 tctaatactt tatcatcgat acttttgatt aatccttgct ctaaagcaat gccaatcagc
    72541 aaagatacaa tacttttcgt tgctgacatg atatgacaag tatcgtcttt tttataacca
    72601 ttccattcat ctgtataaac ttctacccca tccttatatg cgacaatttg acatatattt
    72661 ggttgtcgaa aaccaataaa atcatgcaac tcttgtttat tcataagata ttacctcact
    72721 ttcttctata agcataatgt cattaaaaat cgatttcaat tttttcgatt cttcacgtaa
    72781 tacaggtatt cttatggact agacagcgct ttgggaagat ggattctctg aaaaatataa
    72841 aggttcactt tatgagtgga taaaaaaagc agcaaatact tcgacttctt taatttacca
    72901 acattagtga aaatcgattc atctggagat ttgtaattta gagtccttgt cttgattaga
    72961 ggtgtataaa gctttttatt agacattttt aaattccatt acaatttgct tataaactag
    73021 gtttttaaag gtgactatgg taagataata gagatattat tttgtgatta ggagtgtata
    73081 tatgaataaa aatggagaat ggagtgctgc acgacggata ttcattatat taatggatgt
    73141 tattgtatac aatgcagcaa tatatagtag tttcttactg aagtttcgtg gagagatccc
    73201 attacgaaat ttagaaactt tccaacactc agcaattatt atttcagtga tttttattgc
    73261 gttgaacatt ttgttgggtg catatgtttt ttataatcga atgatcagtg acatcgtgtt
    73321 tgtcacagtc atcggtcagg tattgatgac actagggatc atggtagtga cttttgcggg
    73381 ccgctggttt gcttttccac gtcttgttat cttactttca tttgttctcg gtacgctttt
    73441 cttaagtatc tatcgtattc tcgtatataa actttattta cgcgtaagtc gagataaaaa
    73501 ggtcgtcatt attgggttag aaaaggatgt cgcaccagca atccaaaatt ttggttcaaa
    73561 gaaaaacaac aaacataaag tcgaagcagt cattcttgat aactattatg caaacatcaa
    73621 aaatatgatt aaagacatcg atattgtcta tcttgcttct catatcgaag aaactgaaaa
    73681 aatcaaaatc tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga atacaacatt
    73741 tgaaaacttg acattgatca atccaaatat tatgaatttc gaagatgaaa gtattattga
    73801 agcatctgga tttagaatcc aacctgaata tgaactattc aaacgtttgt ttgatatcat
    73861 cgtttcactt gttttattag ttattgcttc accatttatg ttattcacag caattttagt
    73921 caaagtgaca tcaccgggac caatcatcta taaacaagtg cggatcacaa aaaatcaaaa
    73981 ggaattttca atctataaat tccgctcgat gaccgccaca gccgaagcaa aatctggacc
    74041 agttttagct aagagcaatg atgcacgagt aacgcctgta ggtaaattta ttcgagctgt
    74101 ccgctttgat gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt cgatcgtagg
    74161 accacgtcct gaacgtcctt tcttcgtcga tcagttcaat gcagaaaacc cttattacta
    74221 cttacggcat aatgttcgtg ctggtatcac tgggtatgca caagtctatg gaaaatatgt
    74281 ctctgattac aacagtaaat tacgttttga tttactttat atcaaaaaat attcattgat
    74341 gcttgattta aagatcttgc tgcaaaccat caagatccta tttgataaaa tgtcctctca
    74401 aggacttgaa gaagacgaaa atgtgatctc attggcagaa attgcttttc cgaatgaaaa
    74461 aatctttcgc tagttgggag gcgtaaaaat gaacaatccg aaattcagca taatcgtacc
    74521 agtatacaat gcagaagcaa ctattggtcg aacgattgat attctaaaag aattggatta
    74581 tgaagagtac gaagtcgttt tagtaaatga tggatcaaca gatcaaacac aggaaatcat
    74641 tcaagaactg attgaaaatg atgaacgttt tgaattgatt actacgacca atcaagggcc
    74701 cggattagca agaaacgaag gaataagaca gtcaaaagga gaatatctct tattcttcga
    74761 tgcagatgac acgccagtaa aaacgatctt gtcagattat gatcatttgc tagaaaagag
    74821 tccaaaagcg gatcttgttg ttagttcgtt tgtttttcgt acaatggacg agggaagaat
    74881 cgtttccgaa aaagaaaact tagttacaga atgtcactat ctaactcacg aagagtttat
    74941 aaaagacatg tatcagttaa tgaatgaaca gttgatgtat gttgtatgga acaaatgcta
    75001 tcgtagcgat attctgaaaa ataaccaaat tctatttaaa acttataata gttgtgagga
    75061 tcgaatattt aacttacatt attatgaata ctgtcatcaa gtgatcatga acccaaaagt
    75121 agaatatatt tatgaatttg aaggtgggaa ggggatcact aatcaatacc gacctaacaa
    75181 gttcgaaaca tttaaagaat tctattcatt agcaaatact gtaacagacg atattaataa
    75241 agacggtatg gcggcgcttc ttttaaaagg gacaacttca gttatttttt ctatttatag
    75301 tacctctgca agaacttcaa aagagaagaa agacgaagca aagcagatat tgagtgatcc
    75361 agtgatccga gaagccaagc aaattgctaa gacggattct tcagcaaaaa aaataacaaa
    75421 gttgctttac aatatgccag ctccactatt tttatctgct gtaaaagttg gaagcttcgt
    75481 tgaagtgaaa atgccagggt taatggctgc tttgaagcga gtatattagt taatggagga
    75541 aaatataatg aaaagaattt tgcattttca gggacgaatg ggtttaggtg gagcagagtc
    75601 tttcatgatg aatttatatc gtaaaattga tcgtacaaac tatcaatttg attttttgat
    75661 ttatgaagat tatgcagatg ttcaagacta tcattcagag attgaaagat taggaggccg
    75721 tatttttgtt gtacctaatc ctaagaaaaa tatttttaaa tatttaatag aagtgaataa
    75781 gttactaaaa aaagaatctt tttctatagt tcacaatcag gtttattttg gcgggggaat
    75841 taatttatgg ttagccaaaa agaatggaat cagacagaga attgctcata gccatgcaac
    75901 tgaagatgga aaatctcaaa atattgtaat gaatgtattg cgtaaatttt taaccaaatt
    75961 gttgttacaa aatgcaacag actaccttgc cgttagtcaa gaagctggtg aaagtctgtt
    76021 tcaaaaccat ccctttgaga tagttcataa tggtattgat ttagagcttt atagtaaaaa
    76081 ttcagaagct aaggttaata agagaaaaga attggatatc tcaatgagta cttttgttgt
    76141 gggaaacatt ggacgattag agaaacaaaa aaatcaatct tatttgttag agattttttt
    76201 agaaatacta aaaatgaatt ctgattctta cttgatttta gtaggtgaag ggtcacttag
    76261 aaataaactg aaagccaaag cagtagaatt ggatatagag aaaagagtaa aatttttagg
    76321 agaaagaaac gatattcccg agttattatc tgtgatggat gtttttgtaa tgccttctct
    76381 ttatgaaggt ctgccaatgg ttggattaga agcacaagca gcaaaattaa aattagtctt
    76441 agcagatact atttccaaag atacaaagtt aacaaataat gtggattttg tatcattaga
    76501 tgaaacacca caagaatggg caaaaaaagt aataaaagag ccgtatgcta ataaaataac
    76561 acaagaacta aagagttatg atgtttcgta cactactgaa caaatggaga gtatatatag
    76621 tagagatcat taaaaatgtt caagatattt taggagtgat tatttttgaa aatatgttat
    76681 ttgatattgg catacaatga tattggcaac ttaaagagat tggtcaatcg attgaacgaa
    76741 aatgcagttt ttattattca tatagataaa aaaactgata tagaaccatt tattgaagca
    76801 tttaaatcat atgataatgt tgaattttta tgtaataaaa accgtgttaa aatttattgg
    76861 ggtggttttt ccattgtaca agcagaaatc aatttagtaa agagagcatt acaaaatgag
    76921 aaatatttaa aatatgtatt attatctggt gctgattatc caatcaaaga taatgagtat
    76981 atttataatt attttaaaaa gaatagttca gtggagttta ttcgtggaat agatctagat
    77041 caaattaaac ataaagaatt ttattataaa catatagatg tatatcaaaa acatgattat
    77101 cctagaatta ataggaataa cacaactgca tttaaaattt ttagagctat aattaatcgt
    77161 tgtctgagga tgattaaact tcccccaaaa attagacacc ataaatttga tttgtatcat
    77221 ggttctcaat ggtgggcttt gtcgaaagaa tgcctaacag aattaataca aatgtatgaa
    77281 caaaatcaac aagactacct gaattttaaa ataggtatgt ttgccccaga tgaaaaattt
    77341 tttcatacac ttttttttaa ttcctcgttc aaaaataaaa atgttattgg aggaccagat
    77401 ttaccacttg aattgaaaaa tattgaagaa actactttac aaacttcgaa attagcaaat
    77461 attcatatta ttgatccttc catgaacaaa tggtttgatg aaagggatat agatcaaata
    77521 aaagaaagtg aaaaattatt tgtaagaaaa gttaaatcgg attactctga taaactatta
    77581 aatagaattg ataaagaaat tttgaaagtt ggaaatatag atgaatagaa tagtacctgt
    77641 tttagagaag agtttattaa gtcgagatgc gtttcgagta ataaatttaa tcatttgtct
    77701 gctttatttt atgcctagta cttttaatgt aactaatatc ccgatgaaaa tagcttttgc
    77761 ttggggtgga attatagttt tgtatgaggt ttttataaag aaaaattttt ttaaaatgcg
    77821 tttttcttgg ctactgattg gagcaatgat tagttttatc ttaactataa tcattaacag
    77881 agaaaatttt ttaattccat ctatttataa ttttggatat ttgctgatta ctttgatagt
    77941 aatttttcct gcagactttt cacaaagtga agaggaaaaa aagaaaaaaa tggtgtattt
    78001 taataatgtt ttcattatca taattttttt agctgcgcta atatctatct atcaatttat
    78061 ttttttgatt tcttatcatg tcccaacagg aacgccagga ttacttgcaa gacaaggttt
    78121 tattgaacat cggttatttg gtgtctatac aagtccaaat gttggagcta tgtttggata
    78181 tacttctatt attttaagtt taattagtgt attaatttca aaaataacaa aaacgattcg
    78241 tgttttttat atgatgaatt ttatcgtaca aatattttat attacacttt cttcgtctag
    78301 aggtgcccag gtagtaattt gttctttttt agtattattt cttttgttgt ttgccaattc
    78361 aaaatttaga aagagcatta gagaagtact acctgtaaaa atcaaatgga tggtaagcat
    78421 attattagtt ctattggtat atttttcttt tggtactaat tatgctaagg attttttagc
    78481 aacaattcct gtatcgattg atagaaaagt agaagttata gagtcaaatg aatcagataa
    78541 aactccactt ccagaaaaag aagtaatatt acagcattct agtgataact ccgaaatttc
    78601 agctggtcgt tttacgattt ggaaagctgc gcttaaagtt ataagaccaa ctttgttatt
    78661 tggttatgga gagaccgatt tctatgggaa tagcgatgca aaatttttga aaaaagtaga
    78721 tcttgatact acagatgtga atgaactaaa aagagcacat ggaaacatgc ataatggatt
    78781 tcttcaagta cttgtatcaa gtggtataat tgcttttata tgtatctatg ctttctatgt
    78841 gttaaatatc tttagtttac tcaaaacatt ttttagaagt aaagtgaatt atgcgtttct
    78901 tggtattgtt ttaattttta tactatctat ttttatagat gaaatggtgg aggctcatgt
    78961 acttttcaat aagcgcgatg tcatttcaat tgttttctgg tattatttgg gaagcatttc
    79021 ttacttgtat ttaaaaaagg agaattaata tgaagacagc tttcattgct tggactcctt
    79081 tacatttgat taatatatta aatgtttatc aaaattttta ccgagaatca aaaggagatt
    79141 tatttattta ttctgaattt gaaaacgcta gaagatatag tgagcgtata aaagaattag
    79201 ggctatttaa tgatgttgtt tttgtgaatc accggaaaat cggttcttcc tttaaacgta
    79261 aaattaactt gttaactaac cataatcaat ttttctcgga tgatttatcc gtttatgatg
    79321 aaatttttat acagggggaa aattatttca caaaaatact tttctccaat gtttacaaaa
    79381 aaaatccaga tgtaaaacta aattatatag aagatggttt gggtacttat ttgaagaaga
    79441 gtatattttc aacaagtaaa aacgttcaaa gagtttttaa aatattaaat aaaaatagca
    79501 tcttcaaaaa aaaaattgac gattattttg tctattgtcc ggaattgtta ctttatgatg
    79561 taaaaaatat aaaaaaaatt cccaaaatag atgaaaacag taaattgatg aaaaacatac
    79621 aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac
    79681 ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta
    79741 tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt
    79801 atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg
    79861 acttctcttc aacacttttg attagtccta tatctacaat ttctttttca ccatctatga
    79921 tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat gaatatacag
    79981 cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt gaacattata
    80041 accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta agaaagttgg
    80101 tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc cagtttctgg
    80161 tatactatag ggaatttttt tattaaggga atttcattca taactattcc tatttttgta
    80221 aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt tgtatcaatt
    80281 ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag ttttgactat
    80341 aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact ttcgttaatt
    80401 gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt tgatatgaac
    80461 caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat tagttatatc
    80521 cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc ttttatatct
    80581 acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt tgatgataaa
    80641 tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt agtaatttat
    80701 ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag atacgcttta
    80761 aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc acaattcgat
    80821 agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc ctacattagt
    80881 aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg ggttccatgg
    80941 ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag taactattat
    81001 ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga tttagcaaaa
    81061 tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat aaccttaggc
    81121 tatttctttc agtttttata tagtttacca gttaatgctg aattttatga gaaaaaaaca
    81181 tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt aaacatgatt
    81241 ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac gtatttttta
    81301 ctttttattt ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca
    81361 aaaaaaatat gtatcataat aatttttgta tcaatttttt ctttgatact tcatatttta
    81421 atcaatcaaa tgttgattcg ttacgttttg acatcattat tgataattag tttagttata
    81481 tattttttaa agattaagaa tcgaattata gatgactata attcatagga ggagatataa
    81541 gtgtttaaag gaaaaacaat attagtaact ggcggaactg gttccattgg aagtgaaatt
    81601 gttcgtcagc ttttaaaata tgaaccaaaa gctatcagaa tttatagtag aggtgaagaa
    81661 aaacaatttt atatgcaaca agagctctca aatcacacta atttgagatt tttaattggt
    81721 gatatcagag actctcaaag gattgattat gcatgtcgtg atgtggatat tatttttcat
    81781 gctgccgcaa tgaaacacgt tccagcaagt gaatataatc ctatggaagc tgtaaaaaca
    81841 aatgtgatgg gaactcaaaa tgtaatagat gcagcgatca aaaataatgt aaaacatttt
    81901 gtaggtatca gtacagataa agtagtgagt cctactaaca ccatgggtgc gacaaagtta
    81961 ttgagtgaaa aattaataat agctgccaat cagtataaag gaaaaacaga aacaatattt
    82021 tcttgtgtaa ggtttggaaa tgtcatgggg tcaagtggat ctgttattcc tctttttagt
    82081 aaacaaataa aaaacaataa agatattact ttgacagatg ttgatatgac acgatttatg
    82141 atgtcaatac cggatgctgc aagattagtt ctgtctgctg ctatgaattc aaaatttgga
    82201 gaaacatatg tcttaaaaat gccggcaatc aatattttaa ctttagctca atcattaata
    82261 aaaatatata atgatacaca tgatgaaaaa tatagaggtg aaataaaagt tgtaggtatc
    82321 aggccaggag aaaaattata tgaagagcta atgactacag acgaaattga acgctcatat
    82381 gaaaatgata aaatgtatat cattccacca cactatcatg ctactgacga actgtacaaa
    82441 gattatgaaa aaattaaaat cactgcatac tcttcagatg caacagaatt aatgagtgga
    82501 caggaattta ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg agttttatga
    82561 tgaaatcaga agtatatgta attgcagaaa ttggtgtaaa ccataatgga agtctagatt
    82621 tagctttaga aagtattgat aaagctaaag aatgtggagc taacgcggtt aaatttcaaa
    82681 catttaaaac gaataagcta gtttccaaaa aggctccaat ggcaaaatat cagaaggata
    82741 atcttaaaga ggacagttct caatttgaaa tgctaaaaaa attagaatta tctcaatctg
    82801 attttattag aataaaagat tattgtatac aaaataaaat tgattttttg tctactcctt
    82861 ttgatgaaga aagtgcagac tttctaaaac aaattggtgt acaaggattt aaaattggct
    82921 caggagattt aacaaattta ccttttctta gaaaaataga tatgtatggg ttgccagttt
    82981 tattatctac aggtatggct gatattcatg aggtcagtga ggctgtagat tgtttcatag
    83041 atagtccagt tactatttta cattgtactt caaattatcc ggctagttct gaagatatta
    83101 atttattggc gattaacaca ttaaaagaaa tgttcgggat acccgttggg tactccgatc
    83161 attcattggg atatgatgtt ggtatttgtg ctgttgcgat gggtgctaag gtaattgaga
    83221 aacattttac cttggataag gaactacctg gtccagatca taaagcttct ttagatcctc
    83281 aggaatttgc agattttgta aagcatatta gaaacactga aatattttta ggtgatggtg
    83341 ttaagcgagc aatgccttct gaacttagta caaaagaagt atcaagaaaa agcctagtgg
    83401 tcactgagaa tttaaatgtg ggagatattc ttacagagaa atctcttaca ataaaacgtc
    83461 ctggaaatgg aatagctcct aagtttttat atcaatatat tggcaaaaaa gttaaaagac
    83521 caataagtgc agatagtgtt ctgcttgagg aagatgttga atgaaaaaaa tacttatagt
    83581 aagtggaaca cgagctgatt atggaatata taaacctgtt gctgagaatt tactacaaga
    83641 taattgggat gtatcatttg ttgtttctgg aatgcattta tcccataaat atggatttac
    83701 taaagaattg atcttagctg acggatttag aattagtgga gaagttcaaa gtttgttttt
    83761 agagaacaat aagggaaaca tggctcggtc tgtggcatta gagataaatg gtttcacaaa
    83821 tatatttgaa caaactagcc ctgattttgt attattacta ggggatagag gagaaatgtt
    83881 ggcagctgcg atagcttgtt tgtatcttgg aatcaaaaca gcacatattc acggaggcga
    83941 agtgtctggt actgtagatg aatcaataag acatgccata acgaaaatag catctgttca
    84001 ttttgctgct acggaaaaaa gcaaagagcg tttaataaaa atgggggaag attcttggag
    84061 agtttttccc gtcggagctc ctagaataga tactatttta aataatagat tacctagttt
    84121 tagagatata aaagaaaaat atcatctaaa atttgaaaag aaaaaatacg ttttgcaagt
    84181 atttcatcca gttgtaaccg aactagaaaa tatagaaaaa caaattttag ctttgatcaa
    84241 ttcaatggaa caacaatctc aaccaattat ttgcatttta cctaatagtg atgctggtag
    84301 cgaaattatc agaaaaatgt accagaaata tttaaaaacg gatattattt atattacgaa
    84361 tttagagccg gaggaatacc tgactatatt aaaagagtgc aaatttatga tcggaaattc
    84421 ttcttctggg ataattgaag ctgcatcatt caaaatacca gtaataaatt taggaaccag
    84481 acaaaatgga cgagaacaat ctaaaaatat tataaatgca aatctaaatg aaaatgaaat
    84541 tgaaactgct ttgaataaaa tacaagatcc tcagtttata aaaagcttga aaaatgttaa
    84601 gaacatctac ggtgacggca acagttctag aagaataaca aaaacattga atcaaatatt
    84661 gaatgatggg gaagatatta agtggattca aaaacaaata gcttattaaa atatactagt
    84721 gatccataca ttattattgg ggctggcgga cacgcaaaag tcattattga aaccttaaga
    84781 ttagaaaata agttaatata tggatttttt gatgataagc aaaataaaat gcaagatctg
    84841 aaatatcttg ggaaaataaa cgaattgaat agctgggcgg atagcaaaaa aaaaatctat
    84901 aaatttattg tagctattgg tgactgtgta gtaagaaaaa agattataaa aaaattgaat
    84961 atgccaagtg agcaatatgg aatagctgtt catccaagtg ctatagtggc aaaagaaatt
    85021 tcaattggtt atggaactgt tatttttgcg aatgctgtaa ttaacccaga tgctgtaatt
    85081 ggagagcatg ctataataaa cacaggtagc attgttgagc atgattgtag aattaataac
    85141 tatgttcatt tatctccagg tgtatgctta gcgggtggag tacatgtagg tgtaggaact
    85201 caaataggaa ttggttctca atgtatccag atgaagaaaa ttggatcttg gtgtatgata
    85261 ggcgcaggga gcactattgt taaggatatt ccctcacatt ccttagctta tggcaatcca
    85321 gctaaaatta agaaagaagg aattaatttt gaattctaga attttattat caagtcctca
    85381 tatgggagga acagaagaaa aatatgttgc taaggctttt aaagataatt ggatagcgcc
    85441 attaggtgaa aatgtggata aatttgaaga aagtattaca aaatatactg gttctaaggg
    85501 cggagcttct gcgttaagtt caggtacagc tgccatacat cttgccttaa gactgcttaa
    85561 tgttggaaaa aacgatattg tctttgctcc ttcatttact tttatagcta cagtaaatcc
    85621 tattttatat caaggggctg taccagtgtt cattgactca gaacctaata catggaatat
    85681 gagccctgtt gcactagaga gcgcattaga agaagctgaa agaaacaacc aattacctaa
    85741 ggcaatcatc gtagtgaatt tatatggtca aatggctgat tatgaacgaa ttttagctat
    85801 tgcaaaccaa tttaatgttc cagtgataga agatgcagcg gaatcgttag gttctactta
    85861 taagggaatt tcgtcaggct tatttgggac aattgggatc tattcattta acggtaataa
    85921 gatcatcaca acctcaggtg gaggagctct agtgtctagt gataaaaaat atgttgagca
    85981 ttcaagattt ttagcaacac aagcaagaga tcaagcaccg tattatcaac atagcgaaat
    86041 aggttataat taccgtctaa gcaatatttc tgccggtatc ggacgtggac aaatggaagt
    86101 attgaatgat agagtagcta aacgaagaga aattttcaat cgatataagg aagctttttc
    86161 tggaattgat agaattgaaa tgatgccaga agtggatggc atggtgccaa atcgttggtt
    86221 gtctacttta acgatcaatg attataattc tattgatcaa gtagttaacg taataaataa
    86281 actaaatgaa aaaaacattg aaacaagagc tttgtggaaa ccactacatt tgcaaccagt
    86341 atttcaaggg gtaaaaatgt attaccatga taataatctt agtgtgtcag aaaagttatt
    86401 taatagcggc ttatgcttac cttctggaag taatatgaca gatagtgaac aagaatttgt
    86461 tattgatgag tttaaaaaaa tattcaagta ggtgtgatat tttatgaaca taggaattat
    86521 tggtttaggt tccatgggga aaagaagact tagactcatt caagaacact ttaatgatat
    86581 tttactatgt ggtgttgata ctaatgaggc gagacgaaag gaagttgaaa aggaatttgg
    86641 aataccaact tataatagtc taacagaggc aaaaaatttg tttgaaataa attcagtttt
    86701 tgtttgtaca tcccctataa cccatgaatt tattataaaa gaggctttaa atttagattg
    86761 taatatcttt acggaaatca atttattaaa tgaatattat gatgaaataa tcgaagtagc
    86821 taaaaagaaa gatagattac tttatctatc ttctacattt ttgaagagaa aagaaattca
    86881 atatattaag tctaaagtga cgcataataa aaaaatttct tatcaatacc atgtaggaca
    86941 gtatttaccc gactggcatc cttgggaaga ttatcgcaat ttttttgttt caaacaaaaa
    87001 aactaatggg tgtagagaaa tattcgctat tgaattacct tggattatag atacatttgg
    87061 gaaagttaaa tcgtttactg taatgaccca gaaattatcc aatcttgata ttgattattc
    87121 ggacagtttc actcttatta ttgagcatga aactggtata gttggaactt taagtgtaga
    87181 tgttgtttca agagtagcta aaagaaattt aactataatt ggtgaaaaga ttttaataga
    87241 atgggaaggt aacccggatt ctctattttc atggaatgaa gaaacttcta gtatgacaga
    87301 tattcaattg tacgatgagg ttatgcatga taataattat tcacatacta ttattgaaga
    87361 tgcatattta gaggagatta aagaattttt cacgcttttg gaaaagaaaa tggaacccca
    87421 atatactttt gaaaaagata agtatattat taacttaatt aacgacattg aggtaaatta
    87481 atgatgaaag taattttttt tgggttaggg tcaattggaa agagacatct aaaaaatcta
    87541 ataagctatg gaaaaaagaa ttctattaat tttgatataa cagcttatag tagcagtgga
    87601 aaaaaagagt ttgaagaaat aacgtatatt caaaatagag acgatttgga tacacattat
    87661 gatatagctt ttattacaaa tccgactttt aagcatctgg aaacactaaa tgtaattgtt
    87721 gaaaaagttg attacatatt tttagaaaaa cctgcttttt ctgagagtat tgatcttact
    87781 caatataatt atgattacaa aaatatttat gttgctgctc cactgaggta taaaaatatc
    87841 atggaagttg ttcgtaatag tattcaaagt aaaaaagtat attctgtaag agctatttgt
    87901 tctacttatc ttcctgattg gagaaaaggt actgattata gaaataatta ttctgccttt
    87961 aaatctatgg ggggaggagt agaattagac tgtatacatg aattggatta tattataaac
    88021 ctatttggtt tccctaaatc agtaaaaaaa cagtttggta aatattcgaa tcttgaaatt
    88081 aacagtaatg atttagcaac atatatattg agatacgatg ataaaattat agaaattcat
    88141 ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt agatgaatgt
    88201 atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt taaagaaatg
    88261 cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga aaacgtagta
    88321 acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa aatagctgag
    88381 gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa ggggtttaaa
    88441 aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt agcaatgata
    88501 gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa tacggacagt
    88561 aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt agaacgtaaa
    88621 cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc attcttacaa
    88681 ctagaaagag aagataatat atttgattta attatagatt tagatttgac ttcaccttta
    88741 cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt agataaagac
    88801 gtctactttt cagttgtttc tgcaagacgt aatccctatt ttaatatggt aaaaagaatt
    88861 gaaaatgaag ttactatagt taataaatca agatacacat ctaggcaaca gacacctgaa
    88921 gtttttgaaa tgaatgcctc aatgtattct tttaaacctg aatttctaaa aaaaagtaat
    88981 tatatttttg atgggcaatg tgggataata aaaatgagag attatctaat tttagatatt
    89041 gatagtgaag aagattttaa atggttagaa tttttgtttc ctaaattttt agaagattct
    89101 tcggagcttc aagatatcta taattattta tatcggaggt aaaaggatga gctgttatct
    89161 tatcaccggt ggggctggct tcattggttc taccttagcc aatcatttag gagaagaaca
    89221 tgtggtaatc gtcgtcgatg acttatcaat gggaaaagta gaaaatcttg atatgacgaa
    89281 aaatatcact tttattgagg gagatgtcgc tgagaaatcc ttgatggaag agatcatgaa
    89341 agctaataaa tttgattata tctttcattt agcagcagtt gctagtgtgg cagattcagt
    89401 tgcccgacca gtagaaacac atcgtgtgaa ttttgaaagt gtattaatga ttttagaatt
    89461 gatccgcaag tatcaacctg atttaaaacg gatcgtgttt agttcttcag cggctgttta
    89521 tggggatgaa ccgactttac caaaaaaaga agagtccgtg attcgcccat tgacgcctta
    89581 tgcggttgat aaattcgctg cagaacaata tgtgctagat tattgtcatt tatatgatgt
    89641 acccggtagc gcagttcgtt ttttcaatgt ctatggtcca aatcaaaatc cgaattctcc
    89701 atacagtggt gtgatttcta ttctcgtaga tcgctataag aaacaaatcg taggagaagc
    89761 aacttcattt acattatttg gtgatggaag ccagtctaga gactttgttt atatagatga
    89821 tgtaatccaa gcattacttt tagttgcgaa tgaggaagct gcactgggga aacaattcaa
    89881 tgttggaacg ggtaaagcaa ccacactttt ggaattgatt catgcaatca atgaaattct
    89941 cggtgtagaa ttgacacttg aatacaaaga agagcgttcg ggtgatatcc atgactctct
    90001 agcagatatt actaagatcc agtcaattgg ctatcaaccg aaatatggta ttttaagtgg
    90061 aatgactaat tatttgaaaa ctgaaatcaa ttaaaacata ctaagataag attagagggt
    90121 aaattcaggc tctttgtcaa taaggactga tgagttttgt aaaatcaaat ctgggataga
    90181 a
//
LOCUS       DACEDO010000004        79340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEDO010000004 DACEDO010000000
VERSION     DACEDO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.004"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1087
                     /locus_tag="IWO52_000281"
     CDS             5..1087
                     /locus_tag="IWO52_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0354578.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IWO52_000282"
     CDS             complement(1305..2447)
                     /locus_tag="IWO52_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0354579.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IWO52_000283"
     CDS             complement(2452..2637)
                     /locus_tag="IWO52_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0354580.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IWO52_000284"
     CDS             complement(2991..3236)
                     /locus_tag="IWO52_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0354581.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="IWO52_000285"
     CDS             complement(3230..3631)
                     /locus_tag="IWO52_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0354582.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IWO52_000286"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IWO52_000286"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0354583.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="IWO52_000287"
     CDS             complement(5649..7415)
                     /locus_tag="IWO52_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0354584.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="IWO52_000288"
     CDS             complement(7428..8222)
                     /locus_tag="IWO52_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0354585.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="IWO52_000289"
     CDS             complement(8232..9608)
                     /locus_tag="IWO52_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0354586.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /gene="eda"
                     /locus_tag="IWO52_000290"
     CDS             complement(9728..10375)
                     /gene="eda"
                     /locus_tag="IWO52_000290"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0354587.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="IWO52_000291"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="IWO52_000291"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0354588.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="IWO52_000292"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="IWO52_000292"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0354589.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="IWO52_000293"
     CDS             complement(13042..14673)
                     /locus_tag="IWO52_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0354590.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="IWO52_000294"
     CDS             14977..15648
                     /locus_tag="IWO52_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0354591.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(16373..16708)
                     /locus_tag="IWO52_000295"
                     /pseudo
     CDS             complement(16373..16708)
                     /locus_tag="IWO52_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16805..17257)
                     /locus_tag="IWO52_000296"
     CDS             complement(16805..17257)
                     /locus_tag="IWO52_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354592.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17264..17812)
                     /locus_tag="IWO52_000297"
     CDS             complement(17264..17812)
                     /locus_tag="IWO52_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0354593.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17857..18189)
                     /locus_tag="IWO52_000298"
     CDS             complement(17857..18189)
                     /locus_tag="IWO52_000298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354594.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18203..18433)
                     /locus_tag="IWO52_000299"
     CDS             complement(18203..18433)
                     /locus_tag="IWO52_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354595.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18655..19197)
                     /locus_tag="IWO52_000300"
     CDS             complement(18655..19197)
                     /locus_tag="IWO52_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354596.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(19218..20309)
                     /locus_tag="IWO52_000301"
     CDS             complement(19218..20309)
                     /locus_tag="IWO52_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0354597.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20317..22527)
                     /locus_tag="IWO52_000302"
     CDS             complement(20317..22527)
                     /locus_tag="IWO52_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354598.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22543..25071)
                     /locus_tag="IWO52_000303"
     CDS             complement(22543..25071)
                     /locus_tag="IWO52_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0354599.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(25123..25533)
                     /locus_tag="IWO52_000304"
     CDS             complement(25123..25533)
                     /locus_tag="IWO52_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354600.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(25546..25764)
                     /locus_tag="IWO52_000305"
     CDS             complement(25546..25764)
                     /locus_tag="IWO52_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354601.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25761..26753)
                     /locus_tag="IWO52_000306"
     CDS             complement(25761..26753)
                     /locus_tag="IWO52_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0354602.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(26832..27743)
                     /locus_tag="IWO52_000307"
     CDS             complement(26832..27743)
                     /locus_tag="IWO52_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0354603.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27762..28106)
                     /locus_tag="IWO52_000308"
     CDS             complement(27762..28106)
                     /locus_tag="IWO52_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354604.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28126..28682)
                     /locus_tag="IWO52_000309"
                     /pseudo
     CDS             complement(28126..28682)
                     /locus_tag="IWO52_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28763..29263)
                     /locus_tag="IWO52_000310"
     CDS             complement(28763..29263)
                     /locus_tag="IWO52_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354605.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(29264..29809)
                     /locus_tag="IWO52_000311"
     CDS             complement(29264..29809)
                     /locus_tag="IWO52_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354606.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29806..31062)
                     /locus_tag="IWO52_000312"
     CDS             complement(29806..31062)
                     /locus_tag="IWO52_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0354607.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(31423..31734)
                     /locus_tag="IWO52_000313"
     CDS             complement(31423..31734)
                     /locus_tag="IWO52_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354608.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(31757..33067)
                     /locus_tag="IWO52_000314"
     CDS             complement(31757..33067)
                     /locus_tag="IWO52_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0354609.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(33681..34133)
                     /locus_tag="IWO52_000315"
     CDS             complement(33681..34133)
                     /locus_tag="IWO52_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354610.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(34135..34500)
                     /locus_tag="IWO52_000316"
     CDS             complement(34135..34500)
                     /locus_tag="IWO52_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354611.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(34599..38882)
                     /locus_tag="IWO52_000317"
     CDS             complement(34599..38882)
                     /locus_tag="IWO52_000317"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ0354612.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(39029..39268)
                     /locus_tag="IWO52_000318"
     CDS             complement(39029..39268)
                     /locus_tag="IWO52_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354613.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(39358..39630)
                     /locus_tag="IWO52_000319"
     CDS             complement(39358..39630)
                     /locus_tag="IWO52_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354614.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(39713..39964)
                     /locus_tag="IWO52_000320"
     CDS             complement(39713..39964)
                     /locus_tag="IWO52_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354615.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(40960..41277)
                     /locus_tag="IWO52_000321"
     CDS             complement(40960..41277)
                     /locus_tag="IWO52_000321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0354616.1"
                     /translation="MNIAYLGKVTVPYLMYGEDYKNILPKDERVSDFKKINSFSMGLR
                     MRKIRKDYYLEREEFGKLFSPSISKWSIDRYENGRDIPNTNRIIQYAYIGNLSLEFLI
                     YGI"
     gene            complement(41342..42955)
                     /locus_tag="IWO52_000322"
     CDS             complement(41342..42955)
                     /locus_tag="IWO52_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0354617.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAYYGA
                     YAPLGYKKHPDIKGKLLIDDETKWIIEKIFSLAYQGYGSAKITKILREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNRQSTISFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNKANKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LHKIQKVGNAERIREKKKKASALRKAENRQNEIDRLFAKMYEDRACEKITERNFIMLS
                     GKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEENERIQEIEIYYRFIGKVD"
     gene            complement(43067..43276)
                     /locus_tag="IWO52_000323"
     CDS             complement(43067..43276)
                     /locus_tag="IWO52_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0354618.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            43367..43555
                     /locus_tag="IWO52_000324"
     CDS             43367..43555
                     /locus_tag="IWO52_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354619.1"
                     /translation="MAVREYHYLTAQVIAIKEDKSFTRTQIGYRSLLQGKGHGVSVST
                     ARCLSNVLNCFIQLSKND"
     gene            complement(44147..45313)
                     /locus_tag="IWO52_000325"
     CDS             complement(44147..45313)
                     /locus_tag="IWO52_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0354620.1"
                     /translation="MNDIKFIETLKQKRNACDYSQSRLALELQISRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSKYFIHEDY
                     GMFGYEEQYIYGDISVNASKDSSMGVLLELRGMGCRNLEYVLQARGIDWYSFLSCCID
                     YQGVFKRIDLAINDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTNGDTAKT
                     FYIGSKSSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAEQTIEQLVFSRNPEQ
                     TIANLILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKTKRWLERQVMPSLLMI
                     KEIEKKTGAKYLEEIDRHTRLTEKQELKIKQMTTDIADMIEKDTAVPQRNDGIF"
     gene            complement(45676..46206)
                     /locus_tag="IWO52_000326"
     CDS             complement(45676..46206)
                     /locus_tag="IWO52_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0354621.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(46388..47044)
                     /locus_tag="IWO52_000327"
     CDS             complement(46388..47044)
                     /locus_tag="IWO52_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0354622.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(47171..47644)
                     /gene="dfrF"
                     /locus_tag="IWO52_000328"
     CDS             complement(47171..47644)
                     /gene="dfrF"
                     /locus_tag="IWO52_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0354623.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(48063..48629)
                     /locus_tag="IWO52_000329"
                     /pseudo
     CDS             complement(48063..48629)
                     /locus_tag="IWO52_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(48825..50660)
                     /gene="lepA"
                     /locus_tag="IWO52_000330"
     CDS             complement(48825..50660)
                     /gene="lepA"
                     /locus_tag="IWO52_000330"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0354624.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            50807..52027
                     /locus_tag="IWO52_000331"
     CDS             50807..52027
                     /locus_tag="IWO52_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0354625.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            52047..52541
                     /locus_tag="IWO52_000332"
     CDS             52047..52541
                     /locus_tag="IWO52_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0354626.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            52534..53205
                     /locus_tag="IWO52_000333"
     CDS             52534..53205
                     /locus_tag="IWO52_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354627.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(53356..55965)
                     /gene="clpB"
                     /locus_tag="IWO52_000334"
     CDS             complement(53356..55965)
                     /gene="clpB"
                     /locus_tag="IWO52_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0354628.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(56216..57262)
                     /locus_tag="IWO52_000335"
     CDS             complement(56216..57262)
                     /locus_tag="IWO52_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0354629.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(57273..57545)
                     /locus_tag="IWO52_000336"
     CDS             complement(57273..57545)
                     /locus_tag="IWO52_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0354630.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(57580..58488)
                     /locus_tag="IWO52_000337"
     CDS             complement(57580..58488)
                     /locus_tag="IWO52_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0354631.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(58620..59852)
                     /gene="pepT"
                     /locus_tag="IWO52_000338"
     CDS             complement(58620..59852)
                     /gene="pepT"
                     /locus_tag="IWO52_000338"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0354632.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(59867..60988)
                     /locus_tag="IWO52_000339"
     CDS             complement(59867..60988)
                     /locus_tag="IWO52_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0354633.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(60985..61686)
                     /locus_tag="IWO52_000340"
     CDS             complement(60985..61686)
                     /locus_tag="IWO52_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0354634.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(61830..62270)
                     /locus_tag="IWO52_000341"
     CDS             complement(61830..62270)
                     /locus_tag="IWO52_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0354635.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(62399..63325)
                     /gene="dnaI"
                     /locus_tag="IWO52_000342"
     CDS             complement(62399..63325)
                     /gene="dnaI"
                     /locus_tag="IWO52_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0354636.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(63325..64698)
                     /locus_tag="IWO52_000343"
     CDS             complement(63325..64698)
                     /locus_tag="IWO52_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0354637.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(64721..65212)
                     /gene="nrdR"
                     /locus_tag="IWO52_000344"
     CDS             complement(64721..65212)
                     /gene="nrdR"
                     /locus_tag="IWO52_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0354638.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(65524..65856)
                     /locus_tag="IWO52_000345"
     CDS             complement(65524..65856)
                     /locus_tag="IWO52_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0354639.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(65908..66537)
                     /locus_tag="IWO52_000346"
     CDS             complement(65908..66537)
                     /locus_tag="IWO52_000346"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0354640.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(66501..67337)
                     /gene="mutM"
                     /locus_tag="IWO52_000347"
     CDS             complement(66501..67337)
                     /gene="mutM"
                     /locus_tag="IWO52_000347"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0354641.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(67382..70027)
                     /gene="polA"
                     /locus_tag="IWO52_000348"
     CDS             complement(67382..70027)
                     /gene="polA"
                     /locus_tag="IWO52_000348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0354642.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(70153..70836)
                     /locus_tag="IWO52_000349"
     CDS             complement(70153..70836)
                     /locus_tag="IWO52_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0354643.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(70829..71314)
                     /locus_tag="IWO52_000350"
     CDS             complement(70829..71314)
                     /locus_tag="IWO52_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0354644.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(71621..72955)
                     /locus_tag="IWO52_000351"
     CDS             complement(71621..72955)
                     /locus_tag="IWO52_000351"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0354645.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(73086..73847)
                     /locus_tag="IWO52_000352"
     CDS             complement(73086..73847)
                     /locus_tag="IWO52_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0354646.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(73863..74345)
                     /locus_tag="IWO52_000353"
     CDS             complement(73863..74345)
                     /locus_tag="IWO52_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0354647.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(74365..76545)
                     /locus_tag="IWO52_000354"
     CDS             complement(74365..76545)
                     /locus_tag="IWO52_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0354648.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(76689..77420)
                     /locus_tag="IWO52_000355"
     CDS             complement(76689..77420)
                     /locus_tag="IWO52_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0354649.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(77489..78430)
                     /locus_tag="IWO52_000356"
     CDS             complement(77489..78430)
                     /locus_tag="IWO52_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0354650.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(78610..78978)
                     /locus_tag="IWO52_000357"
     CDS             complement(78610..78978)
                     /locus_tag="IWO52_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0354651.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT        23128 a        16675 c        12275 g        27262 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
    15721 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
    15781 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
    15841 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
    15901 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
    15961 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
    16021 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
    16081 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
    16141 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
    16201 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
    16261 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
    16321 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
    16381 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
    16441 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
    16501 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
    16561 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
    16621 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
    16681 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
    16741 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
    16801 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
    16861 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
    16921 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
    16981 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
    17041 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
    17101 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
    17161 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
    17221 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
    17281 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
    17341 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
    17401 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
    17461 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
    17521 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
    17581 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
    17641 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
    17701 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
    17761 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
    17821 ctccataaaa aataagttac tttttaaata tcttgtttat gaattcattc cccaaaggcc
    17881 ttttaagatt gatacagcag tccgttcact tcttttagac ttgtcataca agtcttttaa
    17941 atgatcccat atcagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg
    18001 gttcattttt tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc
    18061 atcttggatt tcaataatag tatcatcaat tagtttttga atttcttgcc tatcaacata
    18121 ttcaatctta gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc
    18181 ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg
    18241 aaccagtaat tacactatct acaacgccat tccaatttac tctacatttt ccatttttca
    18301 gatttgtcgg tccatacgga tagccttttt gacattcaat gttcggatta taccagatag
    18361 gattatacca ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa
    18421 atccaataca catcacacta tagccaatta gttttttcat ctcgtatcca tctcctaaaa
    18481 aatagaatag ttcttatagt tattcataag acatattttt tccgtttttg ttttatcctt
    18541 tctgtcctat aacaactttt ttcaagtaaa gaatgacata gtttttctta aaaaaacagt
    18601 ctgttttttt ctatttcttc ggtgtataat aaatatgagt gttcattttt tattttaata
    18661 cgcactccgt acataataaa tcttgatcgc atcaatcttt tgagcttctt gatctacttt
    18721 caattgaagt aatacaaatt gagtaacctt attcattgtt tcttctccaa ataacaaata
    18781 ttgatccgga ttctctatat cttgtgtcac ctggtaaagt ttcccagttg ttttcatttg
    18841 tacatgcggg tctacatcaa tactattgtc tttaatacaa cgatctgtca tatagtccct
    18901 cacagcttga ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt
    18961 taagaaattt tttgtcattt catctatttg catttgttgc gctgtctcgt cttctaatga
    19021 accaatttga ctgttacttt cagtactttg aagagttcca cttgtttctt ttactgcttc
    19081 gattgtttct tctgtcgttc tcaattgtgt tttttgtttt tcagaagaaa accaatgaca
    19141 accagtaaga cctatcaaac ataaccaact aaatactaaa attatccctt ttttcacgtt
    19201 tctagtcact gcctttctta tttcacctta cgaaccagac tacgaatttt tgtttgattg
    19261 tacgtgcgtt gataccgagc cataatccgt ccttgctcgg cattctgttc cacactagag
    19321 aaatgttgtc cgccatatga aacatctgtt ataattccgg tgtgaccata gattccagga
    19381 gacaatgcac cacctgcttg ccaattcacc acatctcctg cttgtaaatc acttggtttt
    19441 gggttcatga ttacttgcca accataggtt gtccagttat aatcttcccc tattaacatc
    19501 gcaaacatgt gtccagaacc cattaactgt gggccaccta attgagatac ataccatgcc
    19561 gtaccaccat aacattcccc attatttacg gatcgtccaa tgatccgatc caaaaccttc
    19621 gcattgcctt tactttttcc tgcaataagc gaagtttttt ttgttggtag gctattccaa
    19681 tgcttgatcg caaaagtatc atattccgtt aagttatagg acgttatgat actcattgct
    19741 cctcgataat acggactccc ttcggtaata cctgctgccg catagcctcc acgacctaga
    19801 atcaataaac aacttccagg atctttattg cccacacatt gattaaagtt gctttggctc
    19861 gccaccataa agccataata gtcataacaa gcttttaagt tcggaaactt catatagaaa
    19921 ccaccttcag aaccaccaat tccacgtgca gaaccttgtg ggtagggaca accaggaaac
    19981 caggtaatcc caaaaaagtt cgtatcgttt cttccagatt gtgactgtcc ataagctgat
    20041 tcatagccta attgagaaag aatcacactc ggtaataact gatactttac agctgcacca
    20101 actgctgctt gcgcatgttc atctgtgatt ggaccacgtc ctttaatctc tgaaaaaatc
    20161 ggtaatcctt cggtatattc cccattaaaa gcgacagcta gacctgtagc ttctcctcca
    20221 tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa aaaagggaag aataaagaaa
    20281 agaagacaac ctagacaccc tttcttcatt tcttcctcat cctttcttcg ataatggttt
    20341 ttggactgtt cgtccttttg gtaaagtaaa atcagttcct ggttttgcgt ccaaaatcgg
    20401 cagtgtttta ggatcctttt ctattttttc cgattgctct gttggcttat ctaaagaaga
    20461 aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt ttactttcag acgaacgttg
    20521 ccccggttgt tgcacagaac gatttcctga tagagtggct accgacgaat taggaacaga
    20581 gtattggttc aataactgct gcattgctgg ataactccca tttttcgcct gatttttcac
    20641 ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat tgtggcacgg gtacttttcc
    20701 agaaggcaac ggaatcgatt gaccaacaaa agcgatacct ttatttaaca atggttttct
    20761 ttgattattc ccttgttgct tactcaattg tgccaatgat tgtacctgtt gttttggtct
    20821 tactgaattc atggacgaat ttggaatcaa accatttgaa agaccagaat tccactctgt
    20881 tttcgatttg gtagaagaca tttcaggctc taccccatga taacgaacat gacgaccact
    20941 ataaggcaag gcttgatttg aacgcccacc tagttcaaag ggattaacca cttgcctccc
    21001 ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt tgaaatccat gtatcgctgc
    21061 agaagcgagt ggattcgttg ctcgatacac attccctgct aatcctgcac ctttgattgc
    21121 caaattttgt ccagcagttg atagttgacc aggcagttgt ttcatttgag acaaagaatg
    21181 acttgctatg tttttcgtgt tgtttatagt ttgatctgca gctcctaacc ctagtttctt
    21241 taataaatct ttccgtttta caatgagtaa aattgatagg agtgctttga caaacatatt
    21301 tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg tctactatgt tatagatcaa
    21361 aaatgccacc aaaataatca cactgtaaaa agatttttga aacatcaagc ccaaaaagga
    21421 ctttaaggta ttcatgagca tataatcaaa tactggaaca aaagaagcga gtaatgaaaa
    21481 aggaatcaca agtaataaac acaacaccaa catttgaaag ataaaacgaa tcatgccaat
    21541 cactacaagt ggtaatccaa tacttagatt aagcaatggc accatagaac caacgactgc
    21601 tttataaaca gctttagttg gttgaatata gacacgataa gccttctttt tccctctatc
    21661 ttctcctgca gcagtggtca cttttgtctc aatatcagat aattcttcac ttgacgcttc
    21721 cttggctaga aattcttgtg gttcaatctc tgcttgatac aattcttcta aatttgttgt
    21781 cccataattt aacaatatat acggatcaac aacagccgct ttataataaa gttggcgcac
    21841 tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca ccattgacat cttttgtcgc
    21901 caattcatct agactatttg tagcagaaaa aataaagcct tctacctcca cggacaaatc
    21961 atttaaccct ttggtaaaag cttctccttt actaggtacg ttactaccat agccaaacca
    22021 aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac accatttttg cgtaatgttt
    22081 ccctctaaat atacgaagag taaatagata taacacatac aagacgacaa tccccgcacc
    22141 aataggatcg tatatttttg aataaatagt atccgaaaaa gtgaaaaact ggtgaattaa
    22201 ctcgttcatt gcagctcctt catagagctt ctcaatgaca aaatcaaggc cttgccaaat
    22261 caatttactt aaactaaaga acatattcgt catcattttt agaaaatctg tgccatttgc
    22321 cccaaataac attttgccta atgaaaattc actatcagat aaataagaag aaaaggtatc
    22381 aatatgaaat ttcccaatct catagatcga ttgatccacc tgcttttctg ccgcatacac
    22441 caatccacca acaaaaagca gtccgaatcc aatcgttaaa aaaaacaaaa cgcaccattt
    22501 cttttcctta tgctttatat aagccattgt ttcacctcat ttctattgca tgattaacgt
    22561 atcctgttct tctacttgag agtgacttgt tttttccgtt gtttgtaatg cagcggtcat
    22621 ttcttcaaac aaactgtgta cattgagttt tcctacacgt ccatagggat ctaacatcca
    22681 acactgtcct ttaatcatgt ggctcatttc agctaagttc gcttcattta cttccaaacg
    22741 taaatgattc aaaatctttt ctcgctcact gtcttcatca aaagcaaaga cgacaccaaa
    22801 attccctttt tctttatctg aacctaagtc accaataaat tgtgtatcta acactaattg
    22861 attcatctgg gaacgcccca cacgtgccat tgctgcaata atactacgtc ctgcgttcga
    22921 attattaaat acccatgctt ctgtaaaata aatcgctgtt ttcttactac tatctcgttt
    22981 tccaaacatt tcacaaaaac gacctaaact aatcatcaaa cacaaagact ttcgatccgc
    23041 atctgtatat agttctggtc ggacattgtc atttggtaat ttcaaatctt ggatctctaa
    23101 aatcgttgtt ttggcattaa agtctaatcc cgggttttct ccataagaaa atcctaaacg
    23161 taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca tattctcgaa cctctttttg
    23221 ttccatttgt tctaaatagt cgaccacatg catcaagcca acttgttgtc cggcttcttt
    23281 ttgaactaaa acttcttgga ttccctttaa taccgcccct tttaaaaggt gattggtacc
    23341 taacggactt aactcatcaa tcatatcctg ggctacttgt ttcgcatcta ctcgggataa
    23401 acaaacaatt ggatctaata caccataatt acggggattt gtagcatcta acgtgacata
    23461 actcattttt tgcaaatgtt ctacaaacaa agggtaatgt ttctgataat acgggtcttg
    23521 aatgagtttc gtaaaccatt tgcgcttttc tcctttggga tccacaaata aactttgtac
    23581 attcataaac gaactataca aaaagagcaa gccaactaaa aaggtcttcc ctttaccggt
    23641 ttttccagtt actgctacat gaggcgcatc aaacaacgca cctttgattc cttccgcaat
    23701 ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa attaattctt ctaatggtgt
    23761 tttttctcct cgagaaccac catcccctaa tgtatcttgc cgaccaatat accagccgga
    23821 ttccatacct aattctgata ctgctgaaaa caaccattcg ccaactgctt ctacagttgt
    23881 tcggtgcttc caatgtaact gccgtcccaa tggttgccct aataaacatt tatagaacaa
    23941 ttctcgttga tcattttttg cacggtggat atggataatc gtatgaagtt gttgaataac
    24001 cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg tatacaataa aacatgcgat
    24061 ccaatcgacg acaggttgcc cattatctac tcgttgcttt aaatgtttta ctaatcctcg
    24121 aacagttaat aagcgttcac taggttcttc atcattttga accaattcct tatgctctgt
    24181 tttgaaccgt tccgatagcc aattcagttt taaaccaata cctccctgtc ctttcaagcg
    24241 tggatagtga attttcattc gcaattctgc tgggaaagaa agatcctgag caaattgaaa
    24301 tagatcacaa tttctcatat tttcaggtgt ttccccaata ggcaacaatg ctaaataact
    24361 actccctaac tcatcttcta attgaagaag gcctttatgt tccttcggta tacgtacata
    24421 cgttccttga ttgtctttca gtacttccca attattttgt tcttctactt tgtgagccat
    24481 tcctcgaata tattgcagac gattgagata caacagctct ttttttacta agggacgaat
    24541 agaaaagcta taaagtaatt gtgtcacttc ttttacataa ggatcacttt cagaaaacaa
    24601 agtttccaaa tcatctactt ctaatccaaa taagcgtgct aaacttcctg ctaaatctaa
    24661 ccagattaga tttattacat catgaaaact ctccaccgtt gctttttctt tgactttgat
    24721 tcctaaaata aatgaaggac gatagacttg accttctcgt tccaacaatg tcactgtttc
    24781 ttttgcataa tacgcaccaa ttgcttgact atcgggagaa aatccctttc ctaatgtgtc
    24841 aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc aactccacat cttcataatc
    24901 tgctaaaagc aacagtaaac gagccatcat ttcttttatt tcctgtgtct tttttggtgt
    24961 gttttttgaa attgttcgtg gaaaaacatg atagtaggcc cagatacaat cctctccatt
    25021 taacaaacaa tttttcttga atgcttttac tggttctttt acaagcacca atgaatttca
    25081 cctcccaata aaaaaggaac aaccagttga ttgtcccttt ttctaatcct ctaaataaac
    25141 atacttattc acaaaaagta attcttccaa tgcattgttt gataccttgt cccagttata
    25201 atacacacaa tcaccattca tcatacgaat catataacaa aaccaatccc ataaataaaa
    25261 atgcactttt tttccatcag gttttagttt tgccagtgct aacacacata aataggggag
    25321 tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa atgacccaac caataaatga
    25381 acgtaaaaat aaatattcaa agattacgac caacaaaaaa gttgctgcat tgactaatgc
    25441 aactggaaat ggaagacgcc aattgcttgt aatctgttgt acgcgataag gttccgcata
    25501 aacaatcgaa taatcataag gttcccaatc cacttcacta tcctcctaat ccataaataa
    25561 tttttcaatg attttaccaa acgcatcaaa tacacgtgtt ggatcattta agaagaaata
    25621 aactgcggca cctgctgcga tagatattaa tgcatttcct acttttgatt tcataaaatt
    25681 tcgcaatccc agataaacaa ccaccattat taaaaaatag ccaccttgcg ttaatataaa
    25741 atctacaatt cccttcagtg tcatttccgt tcttctcctt tcctatccaa ccaattatgt
    25801 gttaattttt caacataata ctgatttgtt tttttcgtaa tgacaatcgt catttgttct
    25861 ttttgtaaaa gtgacgtatc accatctcga aagacgaccg ttgcttttac aataaaaggt
    25921 tttttcgttt ctttataacg caatactttc atctctactt gatcaacttt agctgtttga
    25981 ttgagtcctt caggttcttt catcatgtat gacatatctt ttgcattgtt tttcgcatac
    26041 ttatctaaga aatcttccac aaatccttca acatctttcg cttccgacgc atcaaccgtt
    26101 tcgtataatg tgtcatcata agtaatcatt cgttgttttt ctgtattctt tggtagtgcc
    26161 gtaaaataag gataagcaat aatacgacag cccttgtttg aaatagaaaa gggaagattg
    26221 agtacttgtg tgattgtcac acttgtttct ttcttactct ctggattcat cgagcgaata
    26281 tcataagtta ctttataact agcagttgga atggtttcaa tttcttttaa tccaataaac
    26341 gaagcagcag ttaattttcg aacacctgaa acacttcctg gttcttgaat aaagttttgt
    26401 gcataaaact ctttttttaa tgtttcttga cgttgttgtt cttgttcatg attatcgaat
    26461 gtattcatat acaaagcaac aaaacgattc ataaaggatt caatttcagg tgtgtacgtt
    26521 aattctgacc gactttgtac ttctttcttt tgtaattgtt ggatttcatc tttcaaaaac
    26581 gacacttgaa ccttcgtttt catggattga aaaaatgcca agccccctga aaaacaaaga
    26641 aataaaacca aacaccaaat ccatttcact gctttttttg aatggatttt cttttgcttc
    26701 ggtactttct ctgtataaac tttttctttc cgccgtagtt gtcgtttttt cataagctac
    26761 tccttctagt atgtttttac tagtacgtac gattgacaac gataagcttg tttttctgaa
    26821 aaatagtcca ttcaattacc ctcattttct attccttttc tctttctttt atatccaaca
    26881 aatccagcaa tacttgtcaa cactcctcct agaacagaaa gtgcaacatg tatttcttca
    26941 ccagtctttg gtaagacttt tgtttttcca tctgctgttt tgatttcaat attgccactt
    27001 ggaagtttct ttacaggaat cgacaaatcc tttaccaaat tcccttcact atcttggatt
    27061 aatggaatac catcttttac accaacaatt gtttgatcac ctgctacttt taatggggtg
    27121 gttggtgttg gaatatgggt caattcctct acattactag tgacaatcgg tacactcatt
    27181 ccttgagaag tattcgtcgt aatttcaccg cttggggtta ccgatactgt gacttctttt
    27241 gctggaacaa taggtttatt ttcttttgga attgcttctg atttttcaac aggttgtttc
    27301 ggttcttctt tttgttcttc actggtagac gacatcgttg gctgttccgt acttgacgaa
    27361 tcagtattct tttcagaaga actggttgtt cctgattgtt ccggagttgt gactatactt
    27421 gaatccgttg ttgttgaact atgcacacct tcttcttcga tcgaatcagt cggtaaagtt
    27481 gattcaggaa actctggttc tggtgtcact ggctcttttt caggtttctc tgactgcaat
    27541 tgttcaggtt ctgctggttt ttccgttgtt cctccattta gctctgtctc aacatctggt
    27601 aatccttccc ctggcaatgt ttctacaatt tctgacttag gtgtttctac agtttcatct
    27661 gcataaacca atgaactccc taattgaaac gaaaataatg aaattcctag tgttgttacg
    27721 actgctaatg ttgttttttt catgattatt cctccaattt tttatgcaat tcgtgttttg
    27781 ttcatagacg ccgcactaat attatctgaa cgataaaaga actcccgtcc gttttcttct
    27841 aacccaacat atggattatt tttgaataat tgaatcaata gttcactggt gttgtatttt
    27901 ggatagtttg caaaaaaata atactgtgct tgtcgcccat ccgaaaattc aacaagtaat
    27961 tcataacgat accgcttcaa tccttttcgt tgttttaaag gatctctata ccaacttgtc
    28021 catcgttcca ttcgtggata gagatagaca cgatacacat agaacaatgc cataccacat
    28081 accaccaaaa ataataatcg ccacataaaa attccccctt ttcttctacc tttcttgaat
    28141 catcgaacat tcataccctc tatagagatt tgaaaaatcc atcatcaatt gttcatataa
    28201 acgttgttgc aaatagcgat tgtttcgaat acataattct tcatagccat caatcacttg
    28261 atcttctttt tcatagcatc ttgtagctct atgacgctca gcttgcatga cataaccaaa
    28321 ctctgcacca tatcgttcta ctgttaataa ctgttgacta cgcaataatg caatataaca
    28381 atcacaaaaa tgttcaaccg acatttgatt tttctcacgt ttgtgaaata ggtattcttt
    28441 agaaagcaca ttattcgtta aacaatctga taaatagcga taatctggcg gtcggttatt
    28501 ttcatctgtg aatttttccc ataataaact gatttttatt cgttcagcgg ctagatagtc
    28561 ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt ttatcacctg aataaacaaa
    28621 tccatataat gcgaaacgaa ctcttccttt tcaattggct ctcctaaaaa ttccagtgac
    28681 ataccatctt ctccttccta aaaaaaataa aaaagcagaa accgatttgg cttctacttt
    28741 tctttccttt tatagctctt ttttacggaa gaatccgcaa atgacatttt ttcaaaaaat
    28801 caatcggcac aagttcacga aaacttggag caattttgga tagaatcact tccccatctt
    28861 gtaaaataca atcataaaaa tcatcagtta cttgatactt acgcttctta caataattga
    28921 taaaatagta gcgatcattt tctatttcca ttcgttgttc tactaagcgc atacgaacat
    28981 catcatagaa ataagccaca aatcgccctt caagttcatg tttttcaatg ggatactctt
    29041 ctggtaacaa tctatttttt cgtcgtttat acattgctgg ataccttaga agaagttcat
    29101 ctaaacgtct gtcagtatat ggtaactgac gaatcaacga ttctaatcgt tctttataca
    29161 gatagaataa ttggcgcaag caatagtgta ccgcctctgg attttctaat actgttggat
    29221 cacgtgacca atacgcacca acataaatca taattttttt catttagaca atcacaccag
    29281 caatactaat gaccgatcca caataggtag aacaagtggc atagcgacca atttcctcta
    29341 attctatctc aatctcttct aaagaaagat atgtctgttc ttctgttggc tggcgactca
    29401 tttcccaagt gacagtcacc aatgaaattt cttccataag aggtttttcg ctgctaagtg
    29461 ctgccacaca gttttcatat atcttcattt tatcgcagcc ttttttaacc gctaaaatcg
    29521 caggtattgg caatgaaaat cgataaaagc gaatgaattc tagccttcct ttccttcggc
    29581 taaattccaa ctcttcgagt tcttgttcgt atcccttacg aacttctaat aagcgtgcaa
    29641 tttcttgcca gttctctttt tcctgataac gtccagcttt taccgcttgt tcaatataag
    29701 gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc atttaatgct tcgattgcag
    29761 cctttaaatc caacaggcgc agccattctg ttgtcgtata tgcattcatg caattttccc
    29821 tcctgacgtt cccatccatt ctagatagtt ttgttgctcg tcatttagtt cggcattatc
    29881 aatcccttct tgtacaaagt ctcttcctaa aatacgatct gcttcttgta acaactttaa
    29941 tgtcggagct acttgtttca aaaaccagcg ttctgaacga tctaatgaga atttttctgg
    30001 ctttactgta aaatccaatt cttccatcga acgaaataat tttgcccatc gtttatcaac
    30061 aatccgtgca tcactttcca gtctatccca aacaataagt tttgcattaa ttaaaccaac
    30121 gccaatctct cctaaacgtt tcggtgaatc acctaaaata aatgcttcta ctgcttgtgt
    30181 tgctttctca ttggctaacc gtaactcata tcgattataa ataccatatt tcgttaatgc
    30241 ttctaattct gaaatttttt ctcggttcgc aatttcaaaa cgtttctcat aaaaattaaa
    30301 aaagagagga ctctttcgcg atccaaaata caaagataat ccttcacttc gaaaatagcc
    30361 atcatctaaa aaacaaccgc caatattctg gaatctctta aagtccattt ctaataaacc
    30421 tgctttttgt ttttctagta attcaaagag atcgaaatgt tcttccgttt ccaatcccca
    30481 aagttcatct aacgcaatat cgaatcggct acattgcaca cgtgtataac cagcttccaa
    30541 ctttccttga tttttccaaa tggaatatag ccatgttcgc caatctgttt gttgttcacg
    30601 catgacttcc tcatattccc ggcaaccttg tcctgaaaat tgcacaagta cattttctgt
    30661 tacagttcgt tttgaatgcc agaacaccca gatattccca cgaacagaaa gacagtcaaa
    30721 aagatacaag cccgttggac gtgtttcaaa ttctgtcaga tccattcgta atacacgttg
    30781 aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc aaataatcaa tcattgctcg
    30841 aagatctatt ccgcttttca tatacaatcg aaagcgttcc ttgattcgtt gttcaaaatc
    30901 ctctgggatt tttcttttcc cttgttccat ctttctaatg taatcttttg agcagcccat
    30961 caattgcgca aatcttgctt gtgatagcgg ctttttatct ccacttatat tatgaattgc
    31021 acttaacttc tttctgtagc tttttagctc aatcggtgtc attttctcgc tccttttata
    31081 aaataacaaa atcgattttt tttaaccctt tagaaacacc gatatatcaa ctagtccccc
    31141 tcgttttttg ggaaaagtag tacaactgct gaccttagat aatcacttga tacaaaaggg
    31201 tttataaaac ttttttctat gtgttatgtt atttctgccc cccatattat atatcggggg
    31261 gctttacaca aagaggccgt gcgcaagcgc acgacctctt tgtgagctgc acacgctgct
    31321 agcgagtgcg cattataggc ccatgcttcg cagccttaat gcgcaccgcc aggcagagtg
    31381 cggctaccag actacgtcta gagccctccc gatagataat aactacaaaa ttaaatgatc
    31441 taattttgtt atttcactat atttttcagg agaaaaaacg cgataaattg acaaagttaa
    31501 atcctcttct aaatctactc gataatgata caatcgagtc aatcgttcta tatctttttt
    31561 cgttagcaat acttgattaa taacttttcc tgtaaaaaca cgttctttgc gcaaaaagcg
    31621 gtaatcagtt acttgttcaa tacgattcaa ccaatcataa aacgttctgg aagaaagaga
    31681 aggattaatc attcgtattg ctgtagctac tctataataa caactctctg ccatttaact
    31741 tcacctcctt agttgattac aaaatatgcg catcagaaaa acttgttcct tttggatctt
    31801 ttgctaaata catttggcgc tctagttgtt cttgtaacaa ctgattatca tctcccatcg
    31861 tttgatcttg actatcctct tttactgtaa aatcatagac aatcatttca gtaaatttct
    31921 caaattcttt gataaagtga taatctttgt aaacaaatgg cgcaaaaaac tctacaggta
    31981 catcaaatcc tggaaaagca tatccacgac caggttgatc tttgttgtaa aatggcttat
    32041 ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg agacaatcga ccaacagaaa
    32101 tacggaatgt taaattatcc cgaacaccac ttggaaaata ttctgcatct ggtcgttgca
    32161 ttgctaaaat aatgtacatc cctgcttgac gaccctttaa tactaatggc tgaatcaatt
    32221 tatcgactct atttctcgct tccatatcca acgaaccata aaaagctgcc cattcatcaa
    32281 aaaagataaa atgcggtggt aacccataat aagcataatt tttccctggt tcatatttag
    32341 gatctttcat ggttttcata taggcatacc gttgattcat ttcttttaat gcatttcgta
    32401 aacaaataat catttcttca tttattttgt aaaacacatg tcctttgaat acatcgacgt
    32461 cacctaatgc tgctaaatct gccgcttttc catcgcaaat atctactgtc ccaacactta
    32521 aacaagcttg aattaaagaa tacaataaat aggttttccc accaccgatt gcaccagcaa
    32581 tcaacatatt tggtgattga tcatataccc aatccaaagt tttcattaaa tgaatacgtc
    32641 cattttctgc tatacattga tcaataggaa tccgaccaga atctgaaagg aatacataat
    32701 tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg caaaaatact tcagataaca
    32761 aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc atctaaagga aaagaaacca
    32821 ctaattcttg tttcttttga taaaaataaa cttttggtaa tctcattttt tcccttgttt
    32881 gtttctcact aacttgtttc tttgtataaa caaaccgatt gtcaattaac attcgaacaa
    32941 gtaactgacg ccaataaaca gaggcaaaat atccttcttt tagtcgcgac caatgcatca
    33001 accctaaaat taacccacta accagcaaca aatacaacac aatacttatt ccaggaataa
    33061 gatacatttg ccaattcgaa aacgtactta aacgtggata aaatactttc caactaaacc
    33121 aacctaacag aatcaaaaat ggaaacaaaa agaaaccata atagcaaaac cataaataac
    33181 gattgaaata gcgaattcgc cgaccacgat acttgaaaaa atgagctttc aattgtcaca
    33241 ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt ttgacaaaaa gcaactaata
    33301 caggaatatc acatttagta aaaaaacata atcgaaaaag atttaatcga tttttataag
    33361 ggtgtgcaca catattacat attcgtacta tacgaatcca aatcacttcc tgatgatctt
    33421 gtttccactg gtacggtaat gaaaaacgaa caagatcacg taaaaacata cactgcccaa
    33481 taaccgcaca aatatatgca tttgtttgta caaaaatcgt tggattgtct ccaaaatact
    33541 caatcaattg ttggatattt tgataacaag cacaatctct caaagtattt gattggtttt
    33601 atctgaattc aaaatctccc cttccttcta tcttagataa aaagaatagg aagactcctt
    33661 cctattcttt caaaaaaacc ttattttttc tctttttcct tagaatcaac acttggttgt
    33721 ttttgatcta aacctttttc ttgtgattgt ttcatattag gattatttgt tggagcttct
    33781 actttctcta atcctttcgc ataaatcact aaatctgaat attcacgatt gccatcttgt
    33841 gctggaatat ataccacatg atattctgga tcaattaatt tcacaggatc acgtcgttta
    33901 aaaccagtca aatctacatc tgctggaaat ttaactcgtc gtgcaatctt tggttcatct
    33961 ggatttccaa aaactaattg tgctcgctgt gatcgaattt ctcccgtata atcgcctgtt
    34021 tcattatcaa atacacgatc agactcttct aatgcgccaa agcgtacggc tcctaaacaa
    34081 gtttcatcta atgcttctgt aatgtcataa ttatttggaa attttaatcc catcttacaa
    34141 aacctcccca atttttacac cagttaaaaa gacaccaaca tcttgatagc cagttgttcg
    34201 acgctctgtt tgtcccatat catttgtttc acgactattc actcgttctt tacgaaaaac
    34261 aaaataagct tcttcaaatg aaatacgcac ctctttttct ggtaatcgta atcttttatc
    34321 ctcttcagct gtgagataaa caaaaaaagc aggctgttct tctcgcgaac actctgcttg
    34381 taattgaaca tatcgcccag acacaccatt aatttccttt ggtggattag gttcccgtga
    34441 acccaataaa aatacttgac ctaaaaactc tttcttcata cgcaatgctt tttctgccat
    34501 aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa tatttctaca ttacaatcaa
    34561 acaagcggtt aaaaatccta accgcttgtt cctcatgact aatctttggc tttacgtact
    34621 ttataaagtg ttatacctaa agcaatgact aatactacta atccaagaac cataagtata
    34681 ctacttgttt gttcacctgt ttttggcaac attggtacaa cagtagctgg tttaacccgt
    34741 tgttctggtt tcactggttg cggtggtttt ggcgcttcca caatttgaac cgtttgatct
    34801 ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa acgtttcttt ctcatcttcc
    34861 gttttcgctt ggagccattc cgcaaaactc aagccttttc ctgcaagatt gcgaccgtcc
    34921 actaagaatt caaataagaa ttccatcatt tctttatcgg ccgcaaagac tttttccgca
    34981 tgagtgcctt caaatggtgt gccgtctggt gttaagtagc cttgaacaat taagcgatag
    35041 atttgaccaa tcgcaagacc gtctactttg acccattcaa gcacagtttg atttggcaag
    35101 gccaagatct cttttccgcc atttttattc gtcgctttcg tcgtaatggt tggattactg
    35161 attttgatgg tttggtcatt gtcggtaata tctgcgtgag aagccaataa tacgccttct
    35221 ctaaacagat cttcaaagac cacgacttct ttgcctcgca acgcacgtgc gtctagaata
    35281 aagtctagat tgactgtacc atccgttgtt tctgccacaa acgttaaggt acttgtgact
    35341 tcttttccat ctactagcac tggtttttcc gttactttgt ccattagaac accgttgact
    35401 gtgtatgttt ttccaacgac caaatcgtgg tatttcacag cgtctgtaat ggtgactttc
    35461 gttaatggat taacctcttt caagccagtg ggtttatgtg tagccgttgt acgtacactt
    35521 gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt ctttatggtt tgctagttct
    35581 tcatcatttc taaaaagact ttcaaacacg acgatttcac gaccatttat acctgtcaaa
    35641 tcaaaggtaa atggtacgat caccgtttca ttttctgctt tggcaataaa gccaacactc
    35701 gtttctaccg tatttccatg cacaagcaag ggttcaccgg ttgctttatc cattaagatc
    35761 cctttgacca agtatgcttt tcctggaaga atctcggtaa tttcaacggt atcgtttaag
    35821 gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt caaagacagc cgtggtatga
    35881 acggttggat tattcacttt cacactttgg gcttcatcgt tgatatccgc atgtgctgca
    35941 acttcaatgc catcacggaa aagtttttca aaaacaacga tcgtttgacc tttcagaaca
    36001 ctagcattaa ccacaaattc aaggtctttt gtgccattga ctttgtctgc aataaaggcc
    36061 aaggtacttg tgatttcttt gccatcttgt aaaattggcg cattcgttgc cttgttcatc
    36121 aatactcctg aaatggtgta ttcttttccg acaaacaagt tggtataaga aacactgtct
    36181 ttaatgacga tcgtttctaa tggatcaacc actttttcac catcggcttt atttgacgct
    36241 tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag aaaccactaa cgtttcccca
    36301 tcatctggcg cattttgttt gaatgcaacc gtgaatgctt gtttatctgt atttggttgg
    36361 aaactttcta atggggcatt ttccaagaag taatagtcat cggcaactag acctttcact
    36421 aaaatttccc cgttttgatc agtggtatat tcaccaattg cttgattttt accgttatac
    36481 aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt tccattcttc atacttgaat
    36541 aattttacac ttttccgtgc ttgacggttc gcaaaatgga tcactttgat cggttctgta
    36601 tcattggtaa tcgagaacac taatgggttt tgattcaaaa cgtaatttac gggggctttg
    36661 atttcttcca aacgatagcg atcttttcca taagtaagtg taccgttagt taggaaaaag
    36721 cctttttcat tggttttaaa gatatccgtt gtttctgtat catttggaat ctccattgtg
    36781 acataacgat tggctaaagt atcaaaaatc ttgaattctg tattagcaag tggaatcact
    36841 tttcctgttt cttgatccac tttcaccact ttaagacgtt gttcaattac tttatcttct
    36901 aaaacataat ggaaagtttg cccattttca gaaattgtga cttcaagtgg ttcaaatagt
    36961 ttataccctt cttttccttt ggtttcagaa agaatgtacg tatcaaagac aacggtattt
    37021 ttaccaaaag tagcataacc atcattgtct gtcacaacgg ttttcaccac tttgccagtt
    37081 gtttttgacg tcaaagtgat ttcaatgcca gctaattttg gtttgttatc tggattcgtt
    37141 gaatcgacta aatctttatt agcaactttc actaaatcaa tggttccttt gatcacttga
    37201 tcttttgcgg tcgcattgac agtttttcca ggttcaacaa caatttcaat tgcttcatca
    37261 gaaagcacat aaccttttgg cgctttgatt tcttttaaaa catatttgcc aggatcaaga
    37321 tctttaattt ctgcctgtac tttgccgtta atttttttca aggtaatttc tttaatcttt
    37381 ttcccatctg cttgatacaa accatagacc gcattttcta aagaagctgc accttgtgct
    37441 ttcacaccag tttccgcatc ttctttaaac acacgaatgt ttccttgttt tagaatttgt
    37501 aaattaataa cagacgcatt aggttctgtt ggatgtaaca caccaattgt ttgagctcct
    37561 ggacgacgat aggctaatgg cgcaccttga actttaattt tattcaattg aatttttgct
    37621 ttatccggtg cctcttttgt gactgtaatc catacttcat tgccgtgaat agatacattg
    37681 acaccttttg gtttttgaat gtctttcaca tatttcaaaa aatcattttt attctttact
    37741 ttcaacgttt cgccaatttt taccgtatgt gtttgattat taaaagttgc tttttcacca
    37801 aagttattaa tcaaacgttg aatttctgct tttcgacgat cataatcaac acggatattt
    37861 ttgattggga tttttgtgcc caaagcttcc caaatcataa attgtgccgc aatgtattgt
    37921 tcatcggatt tgtctttatt tgtgacatat cctaaataag agatcaagct tgtttttttc
    37981 cgtacatttg aatttttcaa atagtttttc aaagcagtag ctgtataaac ttgaccttct
    38041 gcggctgaga caccaggctc aatacaaaac gccacttgtc cattaatctc aaaacgaacc
    38101 atataattta aagtgaagtt tagattgttc ggactagaag taacaccaat attttcgccc
    38161 caatcaattt tccgaccacc taaaacagct gcattggttg cacggcgaac acgaaccgaa
    38221 cgattggttg gtactgttac taacagtgta ggtgtacctg tttttggaat gttctgctct
    38281 tttttctttt gattttcaat actagccgta gattctttgt ttggtttcgt tggttgattt
    38341 tgactagctg cggttgaaga gattgttttt tcaacgtttt cctcattttt tccttctttg
    38401 tcagcagtag ccgctggttg ttccactgtt tcttctattc gtttcttttc ttccgcttgt
    38461 ttctcagaag actctggctg cacagaaggc gttgcttcgg cagctttttc tccttctaga
    38521 gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt cttcaactgg ttgtgaagta
    38581 ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct gatcgagagt cgttttggaa
    38641 ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag tggcttcacc agctgggacc
    38701 ccggcttctg ctggttggac ttctgtcact tcagaagcga cttgggcaat ggcttccatc
    38761 gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag cagttcccac caagaaacag
    38821 aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat tcattttcgt ttttgcatac
    38881 acaagaattc ctccttttcc tgagaatttt atataataaa acaaaacgcc gttaccaaaa
    38941 gtgataacga cgtgaactgt ctcttctatt tttatctggc gctcttatct gaacacattt
    39001 catcaatgat tttaccctcc cactataatt acagaacaaa aaaagaacct gcttcgtgga
    39061 aatcaactgt ccatttccca attggaatac cagctttttt ggcaacttca ccatcaccaa
    39121 atctcatgcc ataccaatta ctaccttcat ccttaatttc tttccaacca tatgacatag
    39181 cttcatctaa tgaatcaaaa atcatgacac tattgccaat tgttacagga tctggcttaa
    39241 gatcttctgg aagagaaata tcaatcatac cataatgatc cgattcctca gtattattgt
    39301 cattactagc tactggtttg gttgttctac tggcttattt tgatacaaca agtactctta
    39361 ttcttctttt acttctccgt tttcaatttc ccattcaaac cactccgcaa agtcttgctt
    39421 aaaaccatct actcgatacc tattttgtaa ataggcatct tctgacgtcg taggatctaa
    39481 ataggtaggt ttattctctg gacgagcata gacacctacc catgtttcgt aatctccttc
    39541 aaaataacta tctaccaaat agcctttttt tctaagaatc tcccgaactt gatttttaat
    39601 tattgatact tgttcaaaac catcactcat tctatcacct caaataactc tagattctca
    39661 taaatattcc cgacaatttc aatcctatca atgatctcta acaaatcgct acttatgctt
    39721 ccccattcgt ataagaattt cccttccgca aaataaattt tccataaact tcttcatgat
    39781 cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt cttatctttt agctcagtaa
    39841 atgccataag ttcaacatgt tttccaacct cgaaacaatc acaccaccca tcaaccgtta
    39901 cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt attaaacact aaaactttcc
    39961 gcattttttg ctcattaatt aatcacgcgc gaaatctagg aatcacaact tattcgcttc
    40021 ttctttataa ataaaaaaac tccaataatc ggaaattact atcttttcct cattcctttt
    40081 ttaaatcttc atacttcttc ttatctttgt cctaacctaa taacacaaat tttgaagaaa
    40141 aactcagtaa aacctgattc tttttttatc ttcacaacaa ccaacttttt cttcagatcc
    40201 ttttactaga acttgtatac aaaaattgtt gaatgctgat ataatttgcg gctacttgaa
    40261 aaaacgatag atgtaaaaag ataaatagaa tcatttttta ttctatatca gtctatgatt
    40321 cattgcttat caatcaccca acccattcga tgccgttttt tttagagaaa tttaaaaaag
    40381 accgtacgaa tctcgcatta tccgattact tttccagaaa agatccccaa ttctgtataa
    40441 ataaccctat cttcttgttg gtttcatttt ctcatataag aattcagtca atctacccac
    40501 tatgaccatt actacgtcca acacaactga catattaacc acgtaagtat tccctcacag
    40561 atctccgaat ctccatggta tggattccca tcctgacgct gcagaggaca acatatttca
    40621 tgtggctctg tacccttttg atttggaaac actacaaatc aatactcatc tataacttat
    40681 caagcgaact ttcgctaggc ttctagtgat tactaaattg ttggataaca tacatagccc
    40741 attttatcta gctgttattc ataacagtta gttcttgtat tacttcttcc gcttgtgttg
    40801 ccattgagct tgtcaaaaag ttacttagaa aaaagctgcc agtaaagcaa ctttcaacaa
    40861 accctttttg tttcttatca tatcaagaaa cctcctttta taaatttttt ctataataaa
    40921 aaacgtcgat aacttaataa gtatcgacgt aaactatttt tatatcccgt aaatcaaaaa
    40981 ttctaaagaa agatttccta tataagcata ttggataatt cgattagtat tgggtatatc
    41041 tctaccattc tcatacctat ctatggacca ttttgaaatt gaaggagaaa aaagctttcc
    41101 aaactcctcc ctttctagat aataatcttt cctaattttc ctcatcctta atcccatact
    41161 aaaagaattt atctttttaa aatcgctaac tctttcatct tttggaagta tattcttata
    41221 atcttctccg tacataaggt aaggaacagt gaccttacca agataggcaa tattcattag
    41281 tttttcaata tctggaatat taataccgct ttcccttagt taaagatatg aaccctcttt
    41341 atcaatctac ttttccaata aatcggtagt atatctctat ttcctgtatt ctttcgttct
    41401 cctcattcgt tgttgcctca tgaataagga ttttttctat cattgcattt aataatggtg
    41461 ctgtcagttc ctttggtacg gaatactcct tgattaactc aatccacttt tcagcaccta
    41521 tcatatcctg ttccatttta cttaattctt ctcttaggtt ggttatctgc tgttccagtt
    41581 ctatctgctc tttttgatat ttccccgaca gcatgatgaa gtttcgttcc gttatcttct
    41641 cacaggctct atcttcatac attttcgcaa ataaacggtc aatctcattt tgtcggttct
    41701 cggctttcct caaggcactt gcctttttct tcttttcccg tattcgctct gcattgccga
    41761 ctttctgtat cttatgcaat accttttctt cgtcctgctg tactgcctta gcccaatact
    41821 gcaatcgttc caatacagct tgatacagca cgtcataacg aatgtagtgc atagaacaat
    41881 tacctttacc aaactgcccg tagtaactgc aagcataata actatacggt gttttatttg
    41941 ccttatttgt cgcaaacctc atagaccaac cacaatccgc acacttgaca agccctgcaa
    42001 atatcggcgt tgccttttcc tttgtctgtc tacgccttga ttttatctgc tcctgtacac
    42061 gatagaacac ttccttgtca ataatcggtt catgcgtgtt ttctactcga aaccattcgc
    42121 tttcgggttt tcgtactttc ttcttgcttt tgaaagaaat ggttgactgt cgattatgaa
    42181 cgctgttccc gatatagact tcacttttca atatcgcctt tacatgagca atcgtccact
    42241 cataacgctt gctttcgggc tttccctcaa agatatgagc aaaagtacca taccttgtaa
    42301 aattcagcca agacgctgtc gggacttttt cttctcgcag tatttttgtg atttttgcac
    42361 ttccgtaacc ttgataagct agggaaaaga ttttttcaat aatccatttt gtttcatcat
    42421 caatcaacag tttccctttg atgtcggggt gtttcttata tcctaacgga gcataagccc
    42481 cataatacgc accctctgca aattttgtct taaacgctga tttcacttta cggctcgtat
    42541 ctcttgctac ccattcattg atgatgttct taaatggtgc aatctcactt tcgccttttt
    42601 cgctgtctac accgtcgtca atcgctatgt aacggacatt atggctggga aaatacaatt
    42661 cagtatattg ccctgtcata atataatttc tgccaagtcg tgataagtct tttgttacaa
    42721 cacagttgat ttttcctgcc tctatatcct caatcattct ttgaaagctc ggtctttcaa
    42781 aatttgttcc cgaccagccg tcatcaatat attcatcaat cacattcaaa tgatgttctt
    42841 ttgcatatag acgcaacata cttctttgtg tggtaatgct ggaactttcg ccctgtaatt
    42901 catcatctcg gcttaaccta agataaagtg cagtattgta aatctgttgt ttcattgtca
    42961 gttatcctcc tttataacta acccgtgttt acaatacctg cttgcaagta aagtataaca
    43021 cgggtacttt ttgtcattca atctatttct tacaactgca ccgattttat gcgattgtct
    43081 gctttgcttt ttccaacatg acatccgtta gaagttcttc cattgtcttt cccttttcgg
    43141 aaaaatgctc ttttacaaca atctttgtct ttcctctgtt gccaatgcca cacttgccat
    43201 ttttcttttg aattgttttt acagcattat caataacaca tacccccttt ctgtaaaatc
    43261 tttctaagca attcaacggc ttttgtcata gcccacagga gttccacctc tgcatagttc
    43321 ttatgtagcc gtacccattg cgtgcgacgc tctgaacgct caagctgtgg ctgtacggga
    43381 gtatcattat ctgacagcac aggtcatagc gataaaagag gacaagtctt ttacaagaac
    43441 acagataggt tatcgctcgc ttttgcaggg gaaaggtcat ggcgtatctg tttcaacggc
    43501 tcgctgtctg tcaaacgtat tgaattgctt tattcagttg tcaaagaacg attgaaagaa
    43561 gaaaatcatc tttcctatat accgcgttgg aaaagaggag aaatgtaact aaaattcaaa
    43621 actttttccc tttttcacta ctatctgtac agcaaacgtc catttgctaa gttgaaatag
    43681 aaaaatttta acttcttttc atgcaccata tagggatagc aagtatcggt ttgctaagtt
    43741 gatgatgaaa aaagattgtt tttctttcca cgccctaact ccccatacac taccgttcct
    43801 gccaaaaatg gaatgaaaga ttttctttgc tccatatagg gaacggaaac tacggattgt
    43861 taagttaaaa atgaaaatag tttcttcttt cacaccatat aaggacagca accaccgatt
    43921 tgatgacctg ttttcaaaaa aagtttcgat tttctttcct cgcaccatat aaggaataga
    43981 agtcttattt tgttgcaggt ttttcaaaaa aatttcaatt tcttcttaca ttccgtatct
    44041 gtacaccaaa ggaagatttt atgaccttat ttaaaaagtt agatttcctt tcctcgcacc
    44101 atatctgtac acaagtagcc gttttgttgc agattttttg aagaaattaa aaaataccgt
    44161 cattcctttg tggaacagcg gtatctttct caatcatatc tgctatatct gttgtcattt
    44221 gtttgatttt taattcctgc ttttctgtta gtcttgtatg cctgtcgatt tcttccagat
    44281 actttgctcc tgttttcttt tcaatctctt ttatcatcaa taaagagggc atgacctgtc
    44341 tttctaacca gcgttttgtt ttatccatat tgacagcaac aggtgtcata ataaatggaa
    44401 gtgaagtcgt tacattatct aaaaatatat cccataaaat ataatcggga aaatctattt
    44461 gtgtgaggat aagatttgct atggtctgtt cgggatttct tgaaaaaaca agttgttcta
    44521 tcgtttgttc tgcttttcca tttttcaaac gaatttcaaa gcggttctta atgtctgttt
    44581 ttatgccctt gcttttttgt tccttttctt tttcatacaa gcaaaagtaa atagaactac
    44641 tctttgaacc aatataaaaa gtttttgcag tatctccatt tgtacccgat aattttccgc
    44701 tgtctactgc ttcatgggtt cttgaacgtt tccatacctt gttggcataa tagcgttccc
    44761 ttagaatttc tatatccagc aatccgccca tatcattgat tgctaaatct atacgtttaa
    44821 aaacgccttg ataatctata caacagctta aaaaggaata ccagtctatc cctcttgcct
    44881 gtaaaacata ttccaaattc cgacacccca tacctcttaa ttctaataaa acacccatag
    44941 aactatcttt agaagcattt acactaatat ccccatagat atactgttct tcataaccaa
    45001 acattccata atcttcatgt ataaaatatt tgcttttcat tgccagtaca ttttttataa
    45061 tctccaatgc gtctgtggta ggaaaacgaa cacgcagata gtcaatcgtt aaagtgaaag
    45121 gctgtgtaca attacaataa tcaagataat gtagaagtat atgtttcatt tctttactgg
    45181 cttttgtttt tccgttttca atttcattta agttctgcct gctgatttgt agttccagtg
    45241 ccagtcgtga ttgcgaataa tcacaggcat ttctcttttg ttttaatgtt tcaataaatt
    45301 ttatatcatt catgctttat tcctccaatc aaaaaaggca tatactcata ggagcataca
    45361 ccttgtatct tatatttata ttctattgat atgctgtttt agacctgtcg ggtaaaaaga
    45421 atagggtgtg aagtgaaata agttcttata actttataaa tcgttgcata tcagcagttt
    45481 tgtatttgtt tttgctcttg tcttttgtaa tagtaccccc ctgttagata acgggggctt
    45541 tgaacggctc gcaagctcgc cgaaccgccg aagcgaaccg cccccttgca agcatggcgg
    45601 aaaaatctta aggattttcc cgccaacgtg gtcggatcgc tccgtcggtt taacagcttt
    45661 tctgtttctc tctagttatc tacgcatata aatcaaatcg gttattccgt tataagtctg
    45721 tgttttacaa agccttaact ttatttccct gtctatgttg cccaaaagac gaaccccatt
    45781 tcctaacaga gtaggaataa cagaaatgta atatttatca atcatatttt tgcttaccaa
    45841 ctgctgaaca agatttgctc cgccacaaat ccaaatatcc tttcctgctt cttgttttag
    45901 ccgttccaat aataaaacag gattttcact tgtaaaatga atttgttggg aagaattgca
    45961 ctctttatgt gttaatacat aagttgtaaa tttattatat acccattctt gcggagaaag
    46021 ttcagtaaca acctgatgag aagtattcca ccccattaag acagtatcta tatctttggt
    46081 aaattctgta taagtgtcga tgttttcatt gtcatttcca tgaccattta gccaagctac
    46141 tccaccatta ccgtccgcaa tatatccgtc aaggctcatg gcaataaata aaactacttt
    46201 tctcattttt tctcttttat aaatacaaac aggaatttat ttctcttttt tactcataag
    46261 ggaacaactg attgctacaa ataaacccat taaaatccat ggaaatgctg ctcttataaa
    46321 atcactaacc atactttatt cctccttaaa ttccaatttc cttagttcta caaactgaaa
    46381 tttttgccta tgaatgtatc ttgttcttta tacgcaaaca ggcttctgtg tgcataatcc
    46441 aatgtcttga aaacggcatt tgaattagtc cacaaatatc tttattacac caataatcaa
    46501 tcagccaaat tgctttttca ttgtcgttta ctacattcaa cgattctaag tatccttttc
    46561 tttctttcgg tatttttctt ttcaattcat cataagataa ccgttcaagc attttctcag
    46621 tgctttccca tacttcgaaa atatataaat ataattcttc cagattaagc tgttttgaaa
    46681 agtctgcaat ctgctgtttc atgagttcat ttcctgttgt aatgataggt gaattgatac
    46741 gctcttgata attgcctgca aaaaatactt gttcatcttc atttatcact gtatgcacaa
    46801 ctatatcttc aatgcggaaa atatgccaaa tagaataagc aatcgtctta ctatgataac
    46861 catcagcgtt tatgaagggg attgagttaa aatcttctct gcataattct tctttgaaag
    46921 atactaaggt ttgtattaac tgacttcgta aagtaagtaa agtatcaata cccctcttat
    46981 aagtatcttt cttttttatt tgtgcctgca ttgttttatt aagttcagac cattccttat
    47041 tcatattcaa tcctctctca aattctgatt ttatcgcccc atacaaactg gaatttgtct
    47101 tacaacaatt tatatatgca taaaaagcaa tatatttatg aaaacaccct aaatccaaaa
    47161 tttactcaat tcattttttc cttacataaa atgttctcgt atatttcact tcctcaccaa
    47221 gtgtttcccc tatcaaaact tcaaaatcat tgatatcaaa ttctggaaag aatgtatctc
    47281 catcttcaat atttaaatct acttctgtga tatacatttt atctactatt tgcaaagctt
    47341 ccttaaatag tccatatcca ccagatatgt atacatctcg tcctttagcc aataataatg
    47401 catcttctaa tgatttaact gaaactaaat tatctccttg atactctgtt gtggtggaaa
    47461 caacaatatt cattctatta ggcaacggat gaccgatttc ttcataagac tttcgcccca
    47521 taataaccac attacccgtt gttaactctc taaattgctt ttgttctccc tttattttcc
    47581 atggtatatt accattcttg cctataacat tattctttga cctcgcaaca atcaaaccta
    47641 tcatctattt catcctccat aatatgatgt cgtacctgtg attccatcaa gtgcaccaca
    47701 tcttaacaaa ctcaaaatta tctcgaacta accagctcga caaattcctt ttttgtcttc
    47761 atctgcatta tatcatattg ttataagcat tgccatttct tttctgcttt tgaaaagtta
    47821 cttatcagcc tatgttttgt aaagggtgat attcagcatt tctcacgcaa caagcccttg
    47881 acaaaccata gactaataag ttttaaggta atcaagcaga aatgaaatag tctgctgttt
    47941 cacggcaaca aaaaagagat accaacacac ttatgtagta tctcttagtc tagtagcaat
    48001 attcttttgt ttgcaagcag gtaaacatgg gctgtttctt aatatctttt tgtttgggaa
    48061 acaccttttt cccatctact tataacattt ttgcctgtat agatggtaaa gtttttacca
    48121 aatttttcaa gcgtctcttc ccggttcttt cggacttctc gtattctttt tcccataaca
    48181 attttatcgt cttttgataa actaaatatc tcactatcac gattaatctc cttcaaaatt
    48241 tctttttcat tacttaatag aaaaatatcg attttttttt gcatttcttt tgacggattt
    48301 ctctttctat ttaacacata actgagtaat acttcactaa ttcctatatt atcagctaac
    48361 tctttgttaa gtaacttatt catatctctg tatgcaatta gtttaccgat gagaatctca
    48421 atcttatctt cctcatcttt tattagagtt tctatattca gaatcttgct tagagtatta
    48481 tctacgtctt tagggatttt tcttgaccct ttttcatatt taactaaagt actttgtgaa
    48541 atacctgcta aatcagctaa ctttttttga gatagttttt tctcttttct tagagattta
    48601 agaacccttc cacttgaatt atcaaccatg aaaaaagcac ctcctatgag atgctttcat
    48661 catatactga cagctaaaaa tcttcaagaa aaatattcca attttgttaa ttttagtatc
    48721 gccgtgataa ccaaatgaca gtttaaaatc ttttatacag ataatcctca gtgttgcatc
    48781 tactcaaaaa ggtttattta ataggttttt cgattgatta taagttattt cacactatct
    48841 tgctcatcca ttttcagaac ggccatgaag gcttcttgcg gaacttcgac ggagccgatt
    48901 tgtttcatcc gcttcttccc ttctttttgt ttctctaaga gtttacgttt acgagaaaca
    48961 tctccaccat aacatttagc caagacgttt ttacgcaagg ctttgatgtc tgaacgggca
    49021 acgatttttt gtccaatagc tgcttggatt ggcacctcaa attgttggcg tgggattagt
    49081 tttttcagtt tttcaacgat tgcttttcca cgttcatagg caaagtctcg gtgaacgata
    49141 aagcttaatg catcgacttt ttctccattc agcaagatgt ccatcttcac taacttgctt
    49201 ttttgataac cagacatttc ataatccaat gaggcatagc ctttcgtgct tgatttcaat
    49261 ttgtcgaaaa agtcaaagac aatttcagaa agcggaatat tatagacgac atttactcgg
    49321 taatcatcta aataatccat cgtaatgaat tctccccgtt ttcgttgaga aagttccatt
    49381 actgcaccga caaaatcatt tgggaccatg atctgtgctt tgacgaatgg ttcttctaca
    49441 tcctgaatcg ttacaggttc aggaaaatct gccgggttat ctactgtagc agtcgtacca
    49501 tccgttttat taacatggta aataacggat ggtgcagtcg tgatcaattc tagattgaat
    49561 tcccgttcca agcgttcttg gacgacatcc atatgaagaa gtccgaggaa tccacagcgg
    49621 aacccaaaac ctaatgcttg agaggtttct ggttcgaatt gtaaagcagc atcattcaat
    49681 tgcagttttt ctaaagcttc ccgcaaatcg ttgtaacgag aagtatcaat tggataaaga
    49741 ccacaataaa ccatcggatt catttttcgg taacctggta aagcttcagc ggcaggattg
    49801 tctgctaacg ttactgtatc ccctactcgt gtatcttgga cagttttgat acttgctgtg
    49861 atataaccga catcaccgac catcaaaaag tctctagcaa ctgcttttgg cgagaagacc
    49921 ccgacctctg taacatcaaa tgttttgcca ttactcatca gtttgatttt gtctccgggt
    49981 ttgaccatac cgtccgtgat ccggacattt agaacaacac cgcggtaact atcataaata
    50041 gagtcaaaga tcaaagcttt tagcggtgca tctaagtctc cacttggtgc aggaacatat
    50101 tccacgatct gctcgagaat atcttcaatc ccaatccctg atttggcact ggctaataca
    50161 gcttcactag catcgattcc gattacgtct tcgatttctt gacggacacg ttcgggatct
    50221 gctgcgggta ggtcgatttt attgatgact ggcaggatct ccaaatcatt gtctaacgct
    50281 aaataaacat tggccaatgt ttgtgcttcg attccttgtg ctgcatcgac cactaaaacg
    50341 gcaccctcac aggcagcaag gcttcgagag acttcatagg tgaagtcgac gtgccctggg
    50401 gtgtcaatca agtgaaagat atatgtttct ccatttttcg ctgtgtaatt gagttctaca
    50461 gcatttaatt taatcgtgat tccacgttca cgttccaaat ccatcgagtc taacagctga
    50521 tcttgcattt cgcgagacgt cacagtgtgc gtcatttcta aaatacgatc ggccaatgta
    50581 gatttcccat ggtcgatatg agcgatgatc gaaaaattac gaatctgctc ttggcgttct
    50641 ttcattttct ttatatccat agcgtctcct cttttcatat aatcagcact ttccattata
    50701 ccaacgaaaa gcagtaattt aaagagtttt taattagtca gctatagaag ttttttttgt
    50761 agaattttgc tatactgtta tcaagctaat aaatggagat gaaaaaatgg atcaaaaaga
    50821 ttttagagaa aatcttgaat taaaaccggt cactcaagaa catatcgatc aattcaatga
    50881 attgctttcc tatgtgttcc aagtgactga agcagacatc gaggaaagcg ggtttgagaa
    50941 taaacgagct tttatccgtt ccaaaaaacc gattttggaa gtatcgaaag tgttcggatg
    51001 gttccacgaa aataaactga tctctcaaat cgctatctat ccttgtgaag tcaatattca
    51061 cggtgcacgt tataaaatgg gtggtgtgac tggagttgga acttatccag aatatgctaa
    51121 ccatggattg atgcaagatc taattatcgt tgcattggat aatatgcgca agaataagca
    51181 atggatctct tatttgtatc cttacagtat cccctactat cggcgtaaag gatgggaaat
    51241 catgtctgat aaactttcct ttaaaatcag agatacacag ctacctaaac aaatcccagt
    51301 aagtggtatg gttgagcgat taccagttga tcatccagac gtattttccg tttatgacaa
    51361 atttgcccaa caaaaccatg gtgctttatt ccgcagtgat tttcattggg aagagtattg
    51421 gcgtttcgaa aatgaagatg agcgcacagc cgctgtctac tacgatagca accatgaacc
    51481 aagaggtgtt ctgttctact gggtagctga agaaatattt cacgtaaaag aaatgttcta
    51541 tcttgatcaa gaagctcgaa atggcttatg gaactttatt tccgcacatt tttccatgat
    51601 ctactgggta catggagata tttataaaaa tgagccttta gcatttttga ttgaagatag
    51661 tcagatcaaa gagcagatcg aaccatattt tatggctcgg atcgttgatg taaaagaatt
    51721 ccttcaacgg tttccgtttg ttggaacagc tgatgctttt catttcatca ttgaagatcc
    51781 tgtcgctcct tggaataatg gtgtcttcgc tttaacatgg gatgaacagg gacaagtccg
    51841 tgtcttaaat gaacctatag gaaaatccgt tcgactggat atccaaacat tgacttgctt
    51901 gatgatgaat tatcgccgtg cttcttattt ggcaagaatc gaacggttag aaacagatga
    51961 agaaacactg aagtcactag agcgtatcat tccaaacatg gaagcttact ttagcgatta
    52021 tttttgataa aaaggatgat gtttagatga aaatttattt tgccgcacca atgtttgcta
    52081 aaagtgattt gttatataac caacagttag ttaaagaaat cagagagctc tcttctgagc
    52141 tttcgatcta tcttcctcaa gaaaatgaag caatcaatga caaaactgct tatgcagaca
    52201 gtcgaatgat tgctcttgct gacacagaaa aagtgttggc tagtgattta atgattgctt
    52261 tactagatgg gctgacgatc gatgccgggg tagcatcgga aatcggtgtg gcttatgcga
    52321 aaggcatccc agtgatcggc ttatatactg atactcgaca gcaaggcgga acccacccga
    52381 aaaaaatcgc tgcactacaa gaaacagctg aaaatcaatt tcactacctg aacttatata
    52441 caataggttt gatcaaattg aacggaaaag tggtttcttc agaaattgag ttgctttcag
    52501 aagtaaaaag atttttagat ggagggactt tcagtgatta aagaaatcaa aaaggtgcaa
    52561 atagctttac ttgcttttgg tgtattcgtt gtctgctata atttttatga atttatcaca
    52621 caaaaatatt cgacttctca aggaatcaca tttatcgtgg aatctttatt agggattgca
    52681 ttgattttca tgccacaagt cattttgaaa gttttcaaac ttaaaatacc agcagcaatc
    52741 gttttgtttt actggttttt cttgttcatc tctgtctttt taggcacagg gatgcatttg
    52801 atcagtatca tctctttttg ggacaaaatc ttacatgcgg tgagtccaat ggtcctgaca
    52861 gctcttggct atggattgat tggttacttg atgaaagatg ctgagatatc aaaaaccagt
    52921 ccttggttgt tcctcttatt cggctttgcg tttgctggac tttgcggcgt gttttgggag
    52981 ttctgggaat tcttatgcga ccagttctta ggcatgaatc ttcaacgttt tgctgcttct
    53041 gatggcacac tgtttgtcgg acgagcagca ttgatggata cgatgggcga cttgctgaca
    53101 aatacgatag gtgctgcctt aatgggattg tttgcatggt ctcaaagtaa aaaagatgag
    53161 cgttactttg aaagttataa attagaaaaa gtgaaaaata cctaataaaa gaggttgtga
    53221 cagaagcggg cagcttcaag aaatttcggc ttatttttcg aagaaactac ttctggaaca
    53281 ctgtttattt gaaaaaagga ggtctgacac tagttgtgtc agaccccttt tttcttttat
    53341 agtctcaatg gaagattaga cgatttcttc tagctcttct gttttgaaat gaagttgtcc
    53401 atcaagtaaa gtaatcgtta cttttgattt aggcataaca tgtcctgcaa cgatttcttt
    53461 agctagtggt gtttctactt ccttagtgat aaatcgtttc aatggtcttg caccatatgc
    53521 tggttcataa gcattttcag caatccacgt tttggcttca tcactgattg tcagcaggat
    53581 ttcttgatgt tccagacgtt gtgccaattg cgccaccatt ttgtcaacga ttcctttcac
    53641 gttgtctagg ctcaatggtg taaataaaat cgtgtcgtcg attcggttca agaattctgg
    53701 tttgaagttt ccacgtaata gcgtgttgac ttgttcagcc actgcttctg gaatcgttcc
    53761 atcagcagtc acgccttcca gcagtaactg tgaaccgata ttgctggtca tgatcagaac
    53821 cgtatttttg aaatctacta ctcgcccttt tgaatcagtc aagcgtccgt catctaggac
    53881 ttgtaacaag atattgaaga catcgggatg cgctttttcg atttcatcca acaagacgat
    53941 tgtataagga tttcgtctga cagcttccgt taattgacca ccttcttcgt aacctacata
    54001 gcctggagga gctccgacta gacgagacac agcatgtttt tccatatatt cactcatatc
    54061 gatccgtacc atatgatctt ctgagtcaaa caagttctca gctaacgctt tcgctaattc
    54121 ggtttttccg acaccagttg gtcctaggaa aaggaaagaa cctagaggac gatttgggtc
    54181 ttgcaatcca gcccgtgaac gaattaccgc gtcactgacc gcatcaactg cttcatcttg
    54241 accaatcact cgtttatgaa gtgtttcatt caatttaatt agtttttctc gttcaccttc
    54301 tacaagtttc gttacgggga tacccgttaa tctgccaacg acttgagcaa tctcattttc
    54361 ggtaacggac tcttgaacca tcttaatctc gctgtctttt gctttagctt ctaattcctt
    54421 caattctttt tctaattgag gtatcgtacc atgacgcaat acagcagcgc gttctaaatc
    54481 atagttattt tctgcatctt ctaattcgtg ttttgcttta tcgatttcag cacgttttgc
    54541 tgaaacagaa ttgacttctt ctttttctgt ttcccactgc atcttcatcg catttgcttc
    54601 ttcacgaagt tcagctaatt cttcttgcaa atttttcaag cgttttttac ttgcatcatc
    54661 tgattctttt ttcagtgcag cttcttcgat ttccaactgc atcaaacgtc tagtaacttg
    54721 atctaattcc gttggcatag agttcatttc tacccgaatc gttgcactgg cttcatcgat
    54781 caaatcgatt gctttgtccg gtaagaaccg atcagtaata taacggtcag ataaagtagc
    54841 tgctgctact aatgcgttgt catggatgtt aacaccatgg tggatctcaa aacgttcttt
    54901 taatccacgc agaatactga tcgtatcttc cactgttggt tctttgacta ataccttttg
    54961 aaagcgacgt tccaacgctt tgtctttttc catatattga cggtactcat ctaaagttgt
    55021 ggcaccgatc aaatgtaatt caccgcgagc aagcattggt ttcaacagat ttccggcatc
    55081 catacttcct tctgtttttc ctgcccctac gatattgtgg atttcatcga tgaacaagag
    55141 gatacgtcca tcactttttt tgacttcttt caagactgct tttagtcttt cttcaaattc
    55201 accacggaat tttgctccag cgatcaatgc acccatatct aatgaaaaaa tcgttttatc
    55261 ttttaaattt tcaggcacat ctttgcgtac gatacgttga gcaagccctt caacgattgc
    55321 cgttttccct acaccgggtt ctcctatcag aacaggatta ttttttgttt ttcgtgaaag
    55381 aatacggatg acatcacgga tttcttcatc acgaccgatg attggatcca tttttccgct
    55441 tttgacttgc tgtactaaat caacgccgta tttttctaat gctttgtatt gttcttcttg
    55501 gttttgtgag gtcactcttt ctcctcctct catctcttcg atgtttttac gcaattcttt
    55561 ttcagataat ccgtttttat tcaaataaac agttaacgga taatttttta atttcatcaa
    55621 cgctaaaata acgacttccg ttgaaagaaa ctcgtcgccg aagctttcac gtatttggtc
    55681 tgcttcattc aataatcgaa agaaattctg gctgaggttt tgtccatatt ggacgtttcc
    55741 accagaaaca actggatact catccagtag acgatcaatt tcatgttcaa aagattcgac
    55801 atccaatccg gcatccgtat agaaatttcg accaaaatga ttagattgta aaaagatttt
    55861 ccatacatgg gcaatgtcga tatcttgatg ttttcgagtg accgcaatct gttgagcttc
    55921 agcaatcgct tcttgaagcg tcgtagtcat tttctcaata ttcatacagg tataccctcc
    55981 taattaaagg taagatcact tatttctttg atctttacaa aaactattat atcgctttgg
    56041 tcaattttgg tcaaaatata cacatagttt tttgactttt tttctcgact tatataaatc
    56101 ttaaaaaaac aaaattgatt gtatgtatat aaaaaaagag ccaaacaaac gtaaaattat
    56161 cttatccata acgtttttct ttgatttgat aaaaagggta gatcttataa aagacctacc
    56221 cttgattttc tttattctct ttttttactt ctattatttc ttctttgatc actcttcgcc
    56281 gttcagcaat cacatacaca aatccataga gaaatggcaa gataaaggaa ctgaaacgat
    56341 acagtaaaag cgaagaaact gcgtcaactg ttccaacaac tggtctgaat aataacaaat
    56401 agacaaattc aaaggaacca ataccagcag gcgtaggaat aacccctgaa agcactactg
    56461 caaagctgat aaacgaaatg gtcagtagaa aatctatttt aggatggttt tcaatcagaa
    56521 tcaaataagg aagtacatac caaaatacaa gttttaacat attccataga taaatccgca
    56581 atagcgcagt cctgtcttgg ataatggttt ggaccgtttc ccttaaagag tagatctgct
    56641 gattgcatgt atccacccat ttccgcaaac gctcactttt aaataactta ttcgataagc
    56701 tgacaaaaaa tatttgaaca ttcaagctga cggacatcgc caataaaaag gcaacaatca
    56761 cgatcgtcaa aaggatacca gcgatgataa accagatcat tgatggacca ttagaataaa
    56821 aaagagagaa ttggatgacc aagccagcta atgcataagt gatcactgcc agtttgtaca
    56881 taatcatatg gagtgctgtc acccctgctc cctgggatag cttcattcct tttttgttat
    56941 aaaaataaat ctccgagatc agcgttcctg taccaaacga aacgattcgg taaaacgcaa
    57001 tatagctaga ggtcaagaac ccatctttcg ttgtaaaatt cggcgcgaaa ggttcagcaa
    57061 tttctttgat tgagcgccct tcagcgattt ggtaaactgt ccctaatata acagccgctg
    57121 ccaaaacaac aacactggta gacagcaatt cggcaaagat atctgcaaat gattgattga
    57181 ttaaataata aataatggca aaaatcacaa tcagcaaaag cagattcaga atgattttag
    57241 ttttcgttcc tttagacgac atctcctgct ccttaatgga aaaaataaat gagaatcaaa
    57301 tcaacaagta cacctgcagc gaatcctata gtaaacttct ttttccgagt tttatgatgg
    57361 aaaacttttc gagagatcaa tcctcccaaa ccaccgccta acaggctgac aaatatcaaa
    57421 ttattctcag gaacacgcca gccgctagtt ctagcctgat gtttatcata ccccatcaga
    57481 caaaataagt aaaaattcac aatgaaaagg taaaaccaca aaggattgtg taacaataca
    57541 tccattactc ttcttccaac tttctgttaa ctactatttc taagcttttt cttctttctt
    57601 tggtgaataa actgttaatc cttttgatac aacagataat tcaactggaa gatcatctgt
    57661 cgtgtctcca tccgttcgag tgcccacaga tttttcctgc gcttggattt tcatgcgttt
    57721 ggctgtgaaa taagtaatgc cggggatcgt atagaatttg cctctcatga ttctaggcaa
    57781 ataaaaaata aatcggaaaa tatctaagga aggtacgatc gttacatgaa attttccatc
    57841 atctagggtc gctgagtcgt caaaatttgt aaaaccgccc acggagttag acatcgtgac
    57901 tgtcaacagt tgggattttc ctttccaatg ctcctcgtct gtttctaaat ccaaatgata
    57961 ttttttcttc ttcagcaata acttaaaaaa cttcttgata aagatccacg agccgtaacg
    58021 ctgtttttct tcttgactga tccaaactgc tgtatctgct aagattccga ttgtcagtgt
    58081 agagataatg acttcatcat tgacttgacc atagtctatt ttccgtacat tgccttccag
    58141 gatcacgtca gcagcttctt cttttttgag gggaatctca agaacacgtg caaggttgtt
    58201 gacagttcca cctggaagta atccgacatg gtattgagat aatgtttttt ggaacgcttg
    58261 gacgatatga tgaatcgtgc cgtctccacc tataattact aaagtatcag cttctatggc
    58321 ttctgctttc ttgactaatt ccgcatcatc aatcgatggg ccagtttcag ctagttctac
    58381 atataattct ggctgctttt cttttacata atcttgaaac cagcgtgcta atttctcacc
    58441 ctcatctttc cctgaacttt tgttataaaa aactagcagc ttttccattc tttacctcct
    58501 atttactctt caaaaaagag ggcgggacaa ccaattccat tgtcctgccc tttagcctta
    58561 gacagatgac attccatcat tgggacgaac gccttgtcat aagctgttct ttttcgattt
    58621 taactctttg tgttcaattc tgcaattttt acaatcacgt ctgttgcttt ttccattact
    58681 tgtaaagaaa caaattcgaa tcgtccgtgc atattttctc ctccggcaaa tagatttggc
    58741 gtcggaatac ccatgtaaga gatttttgaa ccatctgttc ctccacgaac tggttcgatc
    58801 actggagaga taccaagctc gatcattgct tgtttcgcta attccacgat gctcatatct
    58861 ttttcaatga tttctttcat gttgtaatat tgatcataca tatctacttt gattcgttct
    58921 tgatcaaatc gctgattcaa ctcttcttgg atatgtgtga tttgtgcttt gcgtgcttcg
    58981 aatttttcac gatcatgatc gcgaatgata taactcatag aagcttcttc aggattccca
    59041 tccaatgcca tcaaatggaa gaaaccttcg tatccttccg ttttttctgg aacttcatcg
    59101 gctggaagct gattatgaaa atcgattgct aactgtagag cattgatcat tgtatctttt
    59161 gccgtccctg gatgtacatt tttcccttgg atcgtaatat ttgcttgagc agcactaaac
    59221 gtttcatact gcaactcacc taaaggacca ccatcgactg tataagcaaa atctacatcg
    59281 aattgctcaa catcgaattt atccgctccg acaccgattt cttcatcagg accaaaagcc
    59341 actttgatgg taccgtgagg gatagaagga ttattgatca agatctccat tgcagtcatg
    59401 atttccgcaa tccctgattt gtcatctgct cccagtaatg tactgccatc tgttgtgatc
    59461 aatgtgtggc cttcataatt tttcaaattt ggaaaatctt ttgtattcaa tgtatagcgt
    59521 tcttccgcat ctagtacgat cgtagactcg ccatcatagt tttcaatgat ttgtggattg
    59581 actcctactg cattaaaatc tgctgtatcc atgtgggaaa taaatccaat ggaacgcact
    59641 tcatgatcaa cattgcttgg taatgttccc ataacgaatc cgttatcttc attatagaaa
    59701 acatcagcta agcctaactc ttctaattct tttttcaatt cttgcgcaaa agcaacttgt
    59761 gtctgagtcg atggagtcgt tttgcttgtt gcatcggagc gtgtttctgt tttgacatat
    59821 cgtaaaaaac gtggcaaaag attttcgtac atagtattct ctccttttaa ttaaattgaa
    59881 atgggtttgt atttgtttca gaaacgaaaa agttaatatc ccaattttct tcttgtttcc
    59941 aagattcaaa tttctcgata aatcgttgct tgcataacga ttcaatataa tgaccgggat
    60001 caattgcaat caacccagcg ctttgcatat catgagccgt atgataatag atatctcccg
    60061 tgatatagac atctgctctt tgggcgattg cttgcggata aaatttttca ccgcttccac
    60121 cgcaaatcgc aatgcgcttc acagaggatt ttgcattttt tggctggact attctcagtc
    60181 catctagttg gaacgcttct tttacttgtt ctacaaatgc ctctatagaa atttcttgcg
    60241 gcaattcacc tactcgtcct aacccaaaca tttcgactgg ttcatctata gcgaaaagat
    60301 cataagcagg ttcctcataa ggatgagctg accgcatggc ttgaatcact tgtttttcga
    60361 tagtttccgg caaaataacc tcaacttttg cctcttgaac ttgttctgtc ttccctactt
    60421 tcccaatcgc tggttgtgca ccagcttccg gagtaaatcg accgtgtcca atcgaggtaa
    60481 aacttgtacc ggtataatca ccttgtgtac cagcaccagc tgcagctaat acttcacgca
    60541 ttttctgtgc atgatccaca ggtacataaa cggccagttt tttataatga atctcatgag
    60601 tctttactaa ataactctcc acttcaatac cgagaagttc acaaaaccag tcattcagtc
    60661 catcccaaat aatatccatg ttcgtgtgtg ctgcatagac ggcaatatca tgtttcaaca
    60721 aatctgcata cattttttct tgtggctgat cagtaaccag ccgttttaca ggacgaaaga
    60781 ttggaggatg tttagcaatc agcaaatcga tttttttctt gattgcttct tcgaccactt
    60841 ctggacgaac gtctaatgtc atcattactc gttggattgg cttatccaat gttccgatat
    60901 gcagtccgac tgggtctcct tcttcagcta gccactgagg acaatagctt tcaaactttt
    60961 ggatgaaagt gcgtccgctg attgtcatgg ctttaaaacc tcctcaatca tttggatttc
    61021 ctgctcgatt tgtttttgac gtgcctcttg attagctgct tgtttgagct gttcacggat
    61081 agcttttctt tgttgaagct ctcttgtcca ttttgatttg aagatctcac ttttctcttc
    61141 aagcagaaat ggcccgaaca tcagctccat ttcattgtaa gtaactggtt tttgttcttt
    61201 ttcggctaca atgatttcat atattttttt attttcttca agtatttttt ctgattggat
    61261 tttatactga ttttcttgca gccaagatcg taatgtacgc tctccgatgt ttggctgaag
    61321 gatcaaacgt tcttgtccgc ttaagcgttg attcaacttg ccagcttcta ggatatctcg
    61381 gatcagtgaa cctcccatac ccgcaatcgt gacagctgta ataccatctt gtggctggat
    61441 agcatctaga ccatttgcaa ggcgtacggt gatacggtct tctaatccat cttttttgac
    61501 ttgcttacgt gctgattcat aaggaccttg aacgacttca ccagcaactg catattcgat
    61561 ttttcctctt aatatcaatg caacgggtaa gtaagcgtga tctgatccaa tgtctgctaa
    61621 acgagcggat tcagggacaa agtcacccac tgcggctaag cgctgagata aatctaaatg
    61681 gttcatagct tcactccttc tagataatta tagcattttc caaataaaat aaggagacta
    61741 ggaataaaaa aattcccata tctccttaaa aaatatacct tttcgttcta taaaacaaca
    61801 ttttcttatt tttgaaaaaa tgttattggt tatttgttca atgaaacatc aataatcgaa
    61861 tctccaggaa cttgcggtgg gatagacatc gtcatgatcg gcatctcaac taatgtaaat
    61921 tggactttgt caccgatttt caattcattt atgtccacgc ttattttttc agcagtagca
    61981 tttaagatca ctccgtcatt gctgaatgac gttcccatat tcgctggatc ttctttggcc
    62041 atgacatcga cgagtttcaa ctgaagcgtt tcttcttctg cttctacttg agtgatactt
    62101 tcaatcgttc cgacaaatat gctttgtctc tccgttttcg tactgcttgt ttttactgtt
    62161 tgacccgcat caatcgaatc aacagaacct gtgttgctat ctgcgctacc gcagcctgct
    62221 aaaagaaaaa atagaccaac tacagatata ctagaaaaaa tagccctcat aaaaaatcct
    62281 ccgtttaagt ttctgcatta cttgttgcgg tgattgtctc cggtcatttc tgtccatata
    62341 atgattcgct gacctgcttt tactactagg tcaaatgtca gttggacttg tcaactgttc
    62401 aaccattgcg tcggttgcga ccagtcattt cgatttcttt tgtcaaataa cgtatgcgtt
    62461 ccattatccg tttcgctttt agcggctctt catctccacg ttgagtgacg gacaaatgct
    62521 gttccaattc atccattgta aaattcgatg agaagaaggt tggtaactgt tcctgcatcc
    62581 gatattgaag gataacaccg aatacttcgt ctcgtatcca actactcatc gcatctgcac
    62641 caatgtcatc gatcatcaaa ataggtgaac gtttcactgc atctaacttt tctgccactt
    62701 gatcttttcc gattgcctgt ttcatctcaa cggcaaatgt cggaaaatgg actagcgtcg
    62761 tggtgaatcc agcaatcgct aattctctcg ctgttgctcc taataaatat gtttttccaa
    62821 taccaaaact tcctactaga tacagtcctt tatgataccc ttttggatca gcagtatatt
    62881 tctctacaaa atctagggct tctaagctcg catatcctcg tccatctgta cgttcaaagt
    62941 tttcaaaaga agccgattga atatcttttg gcatatccat tgcttttact cggcttctga
    63001 tttcttcttg tctttgccgt gctaacaatg cttccgttgg tacataagtg acatccacat
    63061 aatgaaaatt caacgtcagc ttcggctcat agccgggagc gatcatcgtt ggatcgtttt
    63121 tttgatattt acgcttttct tgcacgaatt catacaattt agcgtaactt ttttcaatat
    63181 ccgcttgtgt caattcttct tggtgtgcgc ttagaaaagc ttgaacatcc gcatcattta
    63241 agacttcagc gatcatttca gcatactttt cttgataatt gcgtttgctg atgatcctct
    63301 tcaattcttt gcctacgtct tccactagat atcaccctcc ttttttgcca ggtattcttt
    63361 aaaacggcgg tcaatctctg cttttttctc gctgctgatc tgtttttcat cttttggctg
    63421 gttgacccaa tcaggaagct tttcccgtcg aacatttcgc ttgccagttt gataatttgt
    63481 ttgcttcttt tgttttgatt gttttgcttg attacttatt tgccgaatgt gctggatcgc
    63541 ttcttctgct gtggcgattt tcttttgtgc ccattcattt gccactgtat ttacataact
    63601 ggcatttaag ctagcattat tcttgatcac tagtacataa ttcagcaaaa cattgatcac
    63661 gctactggaa agacctgatt gagaaacaag atcttgcagt aaccagtttt cttgtttaga
    63721 aacatatccg cctttttctg acttgattgc ttccagatat ttcatcggtg gaatgctctg
    63781 tgcttctttt atcagctgaa gcgctgcgtt tgtcaaatgt tcgtttgacg agggttcatt
    63841 actgttttca gaaaccgcaa ctgtttcttt tgtttgcttc accggacgtc tctgcattgc
    63901 caaaatggct ttttgcagtt ctttttcaga tactttttgt tcactaaagt cataagattg
    63961 actgatcagt tcgccaagtg tcaattcatc gaaaccgtaa agctgatgat acatgtcgac
    64021 ttgtttgatg attggatcat caagacttgc aatatgtttt tttgccagcc aatcattcaa
    64081 aaactgccaa tcgaatgtcg aatctgcttc aagtaatgct actgattcag attcttcaaa
    64141 agatttattc agcgattcga tgttttcttg attagcagcg tacactgctt cattgaagcg
    64201 ataaacatct aaaaattttt ttgtgatttt ttgatatccc tctgctgtat gccttttagg
    64261 ttcaaagaat tgggtaagct tctggaatcg tcgttcacca actttttcca gaagaagaaa
    64321 agccagaaca gagtctttga aaaatgcttt aggaatgagt ggttctaaca gctcataaat
    64381 aaaacgaggt cccagatcac tatcttcttt aaaataaaca tccagtaagc cgattccttc
    64441 tagctttttt cttgcatcat aaaattgcgg gataccgcag cctagactac taagcagatc
    64501 tgcatgcatc ccttcaggac ctttgccaga ttcttgagag atttctgata acagggtgaa
    64561 atagatactc aaggcttctc cgccgattag tggctgataa agatacagca aggcgttctt
    64621 gctgtcttct ctcagcggag ccgcttttct tccttggtaa atattttttg gttgaagctc
    64681 tttccaagca ttttccaagg aaatgcctcc tttttgtttc ttaattgttt tctttattat
    64741 tctctttatt attttctttt gctttatcca cgatatcttg caactctttt aagaaaacag
    64801 acatatcttt gaactggcga taaacacttg caaaacgaat ataagcgatt tcgtctaaat
    64861 caactaaatc ttccatcaca tattcaccaa ttagattcgt cgatacttcg ttttctccga
    64921 gactgcgtac gcggttttcg acatggttga cgatttgttc catttgttcc atagctacag
    64981 gtcttttttc tgctgaacgg atgagtcctc ttaaaatttt atcgcgattg aactcttcgc
    65041 gttccccgtt tttcttgatt actaataacg gagcagcttc aattcgttca aatgtagtga
    65101 atcggaacga acaattctca cattcgcgac ggcgacggat cgcacgtcca tcatctgctt
    65161 gacggctgtc gattactcgt gaattattat gttgacatct gggacatctc attttgtctc
    65221 acctcttgca tcgcatttta tataaaggat gtgtctttct ttacaactag ttgtattata
    65281 acacgaaaca aatcacaaac gcgactgtcc tacccaaaaa acacggatat aagaacaatt
    65341 ttttctactt tcatgcgtaa ttgtgtcccc tgtccttact aaatttcaat tttccaatac
    65401 tgatcacctt gattactgag aaataactta cacaaaagct gatttagtca tctagcacaa
    65461 aaaacttgaa gcagatgaac accttctcat ctgcttcaag tcacatctcg attttttact
    65521 attttataac ttctcttcga ttcctgtata gtgtttcatt cgctgttcct cgattttttg
    65581 ctcatcggga atgtggtcac tttctgggag cggtgtttta ctacgatcag gtgtcggatt
    65641 ttttcgttct tgagtcaaat cttgccgctt cgcttcttct tctgcctgct ggtcttctat
    65701 ttcatcgatt cttgcttggt actgcttttc tttttcttct gctgtttctg attgcgtacg
    65761 accttcatcg attagatttt ctttattgag catctcgccg attttttctt ttgcgttacc
    65821 tacgacttta tccttttgat tttttaatga tcccatcgct atccctcact ttctatatac
    65881 tcaggatagc aaaattgaca cagatactca aagaaagcga ttagcaaaaa gccaattttc
    65941 gatttgtccc acaagttttt cttttgttcc attgttgtcg aacaaaatat ccgcacgctg
    66001 ctttttttct tcgattggaa gttgactact aattctctgc tgcgcttgtt ctagcgtcag
    66061 ttgattacgt ttcatcaaac gctgaagctg agtttctggc gtagtataaa cgacagctac
    66121 tgcatccata tagtggtcat aatgaccttc gtagagcaat gggatatcta caattactaa
    66181 aggatgattg gaacttagag aggcgatttg atcttttatc gcttttcgga taaacggatc
    66241 taatagctga tcaagttttt gccgtttttc ctttgatgga aaaacaatca tcccaagttt
    66301 ccctcgatct agttgcccat caggctgcaa tacctcttgg ccaaacactg atacaatttt
    66361 cctcagagca agcgttccag gctcaacagt ttctctggca atcacatcgc catcgatcaa
    66421 aggaaatcca tgagactgaa agactttggc tgccgtactt ttaccggtag ctattccacc
    66481 cgtcaaacct aaaacaaatc ctactttgcg atttttagcc tttgacaatt cggacaatag
    66541 tgcgtccccc tttgtgccac ttttgttttc acgatcggcg ttccacaacg tatacatggt
    66601 ttgccagttt gctgatagac atgcagtgct acttgaaatc cgcctgattc tcctaatgca
    66661 ttcaaataac tgcgaatcgt ggtacctccg gcttctattg cacgatccaa aacatcaatg
    66721 atcgatagac gtaattgttc tacctctttg gggcgcagtg tgttcgccgg ttgttctgga
    66781 tggatcttcg cttcccataa tgcttcatct acataaatat tacctagacc agtcactaga
    66841 cgttggtcta gcagcaatgg cttgattgct ttgtgggact ttttcagtcc atttgcgaaa
    66901 tctgctaata agaagctatc tggcaaaggc tcaggtccca atttcatgat tcctcggtaa
    66961 gttgcacttg ctcctttttc gacgattgtc atacgaccaa attttcgtac atcgttgtag
    67021 cgaagttggc ttccatcctc aaaaaagaaa agtacatgcg tatgtttatc gattggtgta
    67081 ttttctttcg taaattgata ttttccttcc attcgcaagt gtgagatcag ttcacaatga
    67141 tcgagatgaa aaattaaaaa cttccctcta cgtccaatgg attggatggt ttcgccaaca
    67201 agagaagcct caaagatggg tgtctctggt tgctcgatga tcttgggcca caagacctga
    67261 actttttgaa tcgtttttcc tactactaac cgttccaatc ctttacgaac ggtttctact
    67321 tctggtaatt ctggcatttt ttcttttcac cttcattata ttgctctgtc ttaaacaatg
    67381 tttattttgc ttcgtaccac gtttttcccc aactgctgtc agtgatcagt ggtacatgaa
    67441 gagaaacagc ttgttccata acttccttca ccaatttatc taatgattca agttcttttt
    67501 ccggtacctc gaagacaagt tcatcatgaa cttgtaacag catcgtagct tgaagacctg
    67561 tttcttttaa tcgtttatcc aattcgatca tcgcgatttt caaaatatca gcagcacttc
    67621 cttggattgg tgtattgata gctgttcgtt ccgcaaaaga acggatattg taatttcttg
    67681 aattgatgtc tggcagatat cgacggcggt gatacagcgt ttcgacgtag ccttggtctt
    67741 ttgcttcgcg tacgattctt tccatatatt ctttgacacc tgggtatttt tcaaagtacg
    67801 tatcgatgta ttgctgtgct gcttttcggc tgattcctag attttgtgaa agtccgtaat
    67861 cagagatgcc atacacgata ccaaaattca ctgcttttgc ctgcctacgc atatttggtg
    67921 tgacatcctc tgccttttcg attccaaata cacgcatcgc tgtgctggca tggatatctt
    67981 gaccttctag gaaggcagct ttcaaatgtt catcatttga gatatgcgca agtacgcgta
    68041 actcgatttg tgaatagtca gaagaataaa tcagccagtc tttttctcta ggaacaaatg
    68101 cctgacgtat ctttctccct tcatctaatc gaatcgggat gttctgcaaa ttaggatcaa
    68161 cagagcttaa tcgtcctgtt tgggtcaatg tctggacata acgggtatgc acttttccat
    68221 ctgattggat cactttcagc agtccctcga cataggtaga ttgaattttt gcaatttgac
    68281 gataggtgag gatatcctct acgattggtg cttgttcacg aagctgttcc agtacatcta
    68341 cggctgtgga atatccagtt tttgtttttt tgataacggg taatcccatt ttttcaaata
    68401 agatcactcc taattgcttt ggtgaattca aattgaattc ttcgccggct tcttcgtaga
    68461 ttttttgttc gatctctctt aagcgagcag agaactcgcc cttcatttct tgtaagcgag
    68521 tcgcatctac tttgatcccg cttatttcca tctctgccaa aatgatagac aatggcagtt
    68581 ccattttccg gaaaagatct tcttggtttt tttctgccag ttcttggctc aattttggtg
    68641 tcaacgcatt gattgcagct acttttcgcg ctaaatgagc aaaaaagagt tcctcttctt
    68701 ctggtaaacc ttttttcgcc cctttgccgt atacagcttc atctgattgg atatcgttgt
    68761 atccgtaatg agcagctatt ccttcgatat ctgtgctttt ttcattcgta tcgagaaggt
    68821 atgcacctag caagacatca aaatcgattc ctttcgtttt tccagtatag cggtttaaag
    68881 caacataagt tcgtttcgca tcatatactt ttttcagccg agtcggatca gtgatccaag
    68941 aatggaacgc tcggttttcg aatacagata gatcgttcgt gacataaatt ttcttttctg
    69001 tcccccacgc tactccgacg atttcctctg tatgataatt gtcgcccatc atttccacgt
    69061 acaacgccat atccgtagta aacatttcct ctttgaattc atgaacgact tcaaacaaaa
    69121 tatcatccat ttttacttct tcttctacga tattcaattt agaaaggaac tgattgaaat
    69181 ccatttcttt ataaaacgga acgagtttat cgagattttt cccttcatac ttcaatgagt
    69241 cgatagagac atccacaggc gctgaagtat tgatggtcgc caattttttc gataagaaag
    69301 cctgctcttt gtcatttatc agattttctt tcattttgct ttgcttcatt tcatcgatat
    69361 gttcataaat tccttcgact gagccatatt gtttcaatag cttgatagct gttttttcac
    69421 cgattttagt cacaccgggg atattgtcag aggcatctcc ggctagtccc ttcatgtcaa
    69481 taatctgttt tggcgtcaac ccatcatatt tttccgctac atgttctggt gtatagcttt
    69541 cgatatcgct gacacctttt acagtgatat ccacttttac ttctttacta gctaattgag
    69601 tcaaatcccg atcacctgaa agcacgacga catcaaattg atctttgtct actttttctg
    69661 ccaaagtacc gataatatca tctgcttcat aattgggcaa ttcgtaatac tgtaccccta
    69721 gtcctgtcaa caaatcacgt atatatggca tttgttcttt gaattcgcca ggtgttttgg
    69781 atcttccacc tttgtactcc gcatacattt ctgtccgaaa agtcgttttt cctgcatcga
    69841 acgcaaccag aacatgagtc ggttgctctt tttgcataac attttcaaac atcgtattga
    69901 atgcatagat tgcatttgta tgaagaccgt ttctattttt gaaacgttca agagaattgt
    69961 gaagcgcaaa gaatgcgcga aaagccacac tgttcccatc aacgagtaat aatttatttt
    70021 ttgtcatctt cttaactcct tactgtaaag ttcttattca ttctaacaaa gaatcctgcg
    70081 gaatgcgaaa agaaaaagcg ggaccatacc aaataattcg gtatagcccc aactgatgaa
    70141 tttttttctt ttttagttgt tccgtccaaa gattctcaga aacgccaaga acaagttgat
    70201 aaagtccaaa tacagcttca atgccatgaa tacagcaatt ccactatgtg ctgttccgtt
    70261 tgtctggaca tagatattac ggatgctttg gttatcataa gctgtaatcc ctgcaaagat
    70321 gacgactaac agtagggaga tgaacagctc aactacgcta cttctcaaaa tgaagatgtt
    70381 caagactagt gcaatgatca ctccgattaa agctgcctgt cctgctcttc caaccccgct
    70441 aagatctctt tttgttgtta ctccgacaac agacatcccc atgaacatca aagtagctgt
    70501 caagaaagct tgcgttactg ttccaatatc gtagtatgca agagtaaccg ctaatgttat
    70561 gccattcaaa agagagtagg caataaatcc accgattgct aagctcggat tcttttgtgc
    70621 ttttacgcct agaaccatta ctaaaatcag ttcaacgatc cacagaccca caaagcctag
    70681 tggaaaatta ttgataaaag ttgctaattg caaaggaaat acccaaagag ccaagtaaga
    70741 agtgactgca ctaagggcaa gtcccatacc taaaaatcca taaacttttg cataaaaacg
    70801 attgatacct gacattgatg tacttggatt attcattcaa ttcaccttca ctttcgattg
    70861 ctatttccaa ttcaggaact tgtggcggtt ggaccataac gacagcatcc aacacacgat
    70921 cttcgttcgt attctccgct tctacagaaa tcgtcaccac atccttcatc ttatctactt
    70981 ttatgacctc aaactgaaaa gtcagtgttt cataatggaa aaccggttca ataaaattga
    71041 cagaaaaatt gacaacatgt gatcctggtc caggaagatg ctttgaaatt gcacttgtga
    71101 tgatccccat cagcatcact gatggaacga ttggctgttg atattctgtt ttttgtgcaa
    71161 agtcatgttg gatatacaaa ggatttgcat cattcgtcaa tcccaaatac agcaaaagct
    71221 ggttatcttc aatagattct gttaaggata acgagtcgcc ttcctcgatt tcatttattg
    71281 tttttcctag ttttctcggc ttacctatgt tcaagataga aaccctccag tttattttta
    71341 atcatacatt attgtaccaa aagtagcgta aaaggcaagt tctgctccct tgatttaaca
    71401 gattcctata gtttacagag gtcgtgaaaa agctgtcctg catcaaataa taagaacagc
    71461 caagaaaaaa cagcttctca tgtttttgat ttgattgggc ttattaccgc agatgcaagc
    71521 ttttgaacac cgtttatcat aagttgcaaa aaagggggct tggcaagatt tttgccactc
    71581 cccctctttc tatttttata aggacactgc agtaattgta ttacagtaca tttttcgtcg
    71641 tgctgcttaa aagtttttca tacgcttgtt caaatttttg aacatctcca gcacccatga
    71701 agattactac tgcatcatga taatctaaca atggagatac attattttct ttgatgactt
    71761 ctccaccttt tttgattttc gctccaaggt cttcaatctt aacgtttcct tgttcttccc
    71821 gagccgaacc aaaaatatcg cataagtaaa ctttatcagc taaatcaagt gcttctgcaa
    71881 attcatccat caatgcaatc gtacgcgtaa acgtatgggg ttggaaaact gcaattattt
    71941 ctttatctgg atatttttgg cgtgcgccat caatcgttgc tttgatctct gcagggtgat
    72001 gcgcataatc atcgactacg gtcatgtctg ctacgatctt ttcactgaat cgacgtttta
    72061 ctcctgggaa agtcagcatt tcttctgcaa cttctttcag atccagtttt tcgaaataag
    72121 ctactgcaat aacacctaaa gcatttaaga tattgtgttt accgaatgct ggtacagtga
    72181 agtgcccaac aaattcatcc ccatgataga catcaaaagc agaaccagaa gttgtgcgtt
    72241 cgatgtttcg tgcttggata tcatcgtttt ctgtgacacc ataataataa atcggcacat
    72301 ttgcttttaa tttgcgtaaa taagcatcgt caccatacgc aaaaatagct tttttcactt
    72361 gtccagccat tgtttggaaa gctgtaaata catcgtcgat acttgtataa taatccggat
    72421 gatcaaaatc gatattggtc atgattgcat agtctggtga ataggcaagg aaatgacggc
    72481 gatattcaca ggcctcaaat gaaaagaatt ccgcttgcgg atcaccatgt cctgttccat
    72541 ctccaattag ataacttgtt ggacggacac cactcaatac atgagagagc agtccagtcg
    72601 tacttgtttt tccgtgagag cctgttacag cgatactcgt ataattttgg ataaaatgac
    72661 caataaagtc gtgatagcga atcacttcca aacctaattc ttttgctcgc tggatttctt
    72721 catgtgaatc aggaaatgca tttcctgcaa tgatcgtcat ccctggtttt acattatctg
    72781 cgttaaatgg cagaatcgtg atatttgctt tttctaaatc tctttgtgta aagaaatatt
    72841 tttcaatgtc tgatccttgg acatttagac cttgttcatg taaaacaaga gccaaggagc
    72901 tcattcctga acctttgata ccaacaaaat gatacaattt attttgattt tccatttgaa
    72961 tcctcttctc ttatctagcg tccattcttt tagctattac attcaacacg ctcctcatta
    73021 tcttataaac gattgaaaat ttcaaccaaa tttatttgga aaggaggatt cgcttgctaa
    73081 aatggtcaag tacgagaaaa atccgttttt attgactctt taattctggt gtaagaagac
    73141 caaacttcct ctgatatatc aatgtgagga ataaaatctt ttcctttcgg accatagccg
    73201 ttctcatcgt catggagacg ttccatttct cctaatacta gcgcatggaa atattcctta
    73261 ggccattgat gattcagttc ttttgctgta aagatgatct cttcagaaaa gtgaaggatt
    73321 tccggcacaa atggcacagc attcgcgaaa atagtttgtt cattttgcca tactttctca
    73381 aaagttgctc gagtcctgcg ttgcagtgtc ggatcattca tcaataagat tttttctggt
    73441 acagctccta aggaatgaag aatctccagt gaaaaagtgg cgttctctcc agaatttgta
    73501 gacttcgatt ctatcagaaa agccttatcg ggcaaatcat agacttcttt tagatactga
    73561 cgacagattt cactttcact catgccatct tcaaaatgaa atccttgctt ttcaaaattc
    73621 tcataaagga ttctagtcgc atgccccacc cctccgacca aaaacaattg atctacttgg
    73681 ccatttaaat acatttgtgc tgctttatca gctaagatag gaagacagtt tccagctaaa
    73741 ataagtgtag gtgccgtcag ttcctcaaaa tttccctctt caggtgcggc taaaaaattt
    73801 aatagctgat tccagtcttt tagattcctc tgctcacttt tttccaaagg ccttctatcc
    73861 tttcatctga ttttttgtgc ttcattaaag tcttctcttt ttgtctcaat ccatttgatt
    73921 tctccatgac atcttccaca acgatatttg cgtacatcga ttcttctctt gcggcagatc
    73981 gtggcagaac aatcttggca tcgataatac tccatcttta tctgtttctt cgtcaaggct
    74041 ggtgcatagc gcaaccctcc tgtttttttc aaaagatact tgaaatccat atctttatgc
    74101 tgataccctc ttccagctag atgaagatga taatggcaaa gttcatgttt gatgattcct
    74161 gcaatcgtct cttcgtctga ctcttcaaat agcttcgcat taaaatctaa atgatgatcg
    74221 tttaaatgat agcgcccgcc agttgtacgt aagcgtggat tgaacattgc ttggtgcttg
    74281 aaaggaagct ggaagctttt taatgatatc tcttctacta actgctgcag ttcttcttgt
    74341 gtcaattttt gttactcctc attgttactc ttcataaggg gaaagcatcg tcaaactgat
    74401 tctgcctttt ttcgtatcga cttgttctat ccaaactgtt acgatatctc ctactgacac
    74461 gacatctgta ggatgcttga cgaatttttt gctgagtttc gatatatgga caagtccgtc
    74521 ttgcttcaca ccgatatcaa caaatgcgcc gaagtcaatg acattacgga ccgttccagt
    74581 caattccatc cctggtttca agtcttccat actcaataca tctgtacgta ataagggcgc
    74641 tggcatctca tcacgcatat ctcgtccagg ttgtgtcagc ccagctaaaa tatcggctaa
    74701 cgtttcttct ccgactgata ggctttcagc cagtttctct acggataatc gtgtcaattt
    74761 ttctactgct tcttctgtac caagctcttt ttctgaaagt tggactgaag ttaatatttc
    74821 ttttgcaata gaataacttt cgggatggat acctgtgttg tccaaaatgt ttttcccacc
    74881 agggactcgc aagaatccga ttgcttgttc atatgctttt ggtcctaagc gtggcacttt
    74941 tttcaattga gttcgtgcgg tgaattctcc gttttcttca cgataaatga caatgttttg
    75001 ggcagttgtt ttattcaatc ctgatatatg ctgcaagagc tgcgggcttg ctgtattcac
    75061 atccacacct acttggttaa ccgctgtttc cacaacaaaa tccaattgtt cagccaaacg
    75121 tttttgagaa acgtcatgtt ggtactggcc tactccgaca gctttaggat cgattttgac
    75181 taattcagct aaaggatctt gcaggcgacg ggcaatagaa acagcacttc tttcttcaac
    75241 ttgtagatgc ggaaattctt ttcgtgcaat ttcgctagcg gagtaaacag aagcgcctgc
    75301 ttcgttgacg atagcgtaat aggttttatg gtcggcggat ttcaactgct cagccacaaa
    75361 aagttcagat tcccgactgg ctgtaccatt tccgatagct accatatcta cttgatattt
    75421 attaattaat tggataaaag ctggtccagc agcttctctt tttgcttgcg ctgcaggttt
    75481 atgaggataa ataacttcga ttgctaaaac tttccctgtc gcatctacga ctgctagttt
    75541 acatccagta cgataagccg gatcaaatcc caggacaact ttacctttta gtggcggttg
    75601 caacaaaaga ttacggaggt tttcgccgaa aatagcgatt gcttgctcat ctgccttttc
    75661 agtcaattca ttgcgcagtt ctcgctcgat tgctggctga ataaagcgtt tataactatc
    75721 ctgataggct tctctgacga atgaagaaga aggagaggca ggatttttga ctatttggcg
    75781 atccaagtaa gctaaaattg ctgcttcatc tacttgaaag aatactttca gaatatcttc
    75841 tttttctcca cgattagtag ctaatattcg atgagaaacc attttatgga cgggttctgc
    75901 aaagtcataa tacatttcat atacgccttt ttcatctgct tgctcatcct tgacattgct
    75961 gacatacata cctttgttat acgtataaga ccgaatccaa gtccgaaatt ttgcattatc
    76021 gctgacttgt tcagcaataa tctcatgagc accgtgcaac gcttcttcgg cagaagagac
    76081 ttctttttct ttgtcaatat atttttctgc ttcagattgc acctctccat ctgtaagctg
    76141 catcagccac ttcgcgagtg gttcaagtcc tttttcttta gcaatcgttg cttttgtccg
    76201 tcgtttttgt ttgtatggac gatacaaatc ttccacttgt tgcattttca cggcttgggt
    76261 gatttctgct tctaactcgg gagtcagttt cccttgttca tcgattagcc gaatcacttc
    76321 agttttacgt ttttctaagt tttcaagata ggcataccgc tcttcgattt cacgaatctg
    76381 gacttcatca agacttccag tcatttcttt tcggtaacgc gcgataaaag gaacagtgtt
    76441 tccttcattt aacaaattaa gtacagtagt caactgctta ggacgatagt ctgaaagttc
    76501 tttttgtaca agctgaatga tcgtctggtt caaattttcc gtcattaaaa tgcctctttt
    76561 ctgctaaaaa ttgcttcttt attctatcat actttttatc ctgggttaaa ctactgaaag
    76621 aaacaatatt ctctataaaa ataaaaagag ccgcaacaaa gtcacagctc cttcattctt
    76681 tccattagtc agattccatg ccaaggtcct tcgttgttct gatattcaat atcttgaaga
    76741 ataccttcct tgtcaaaaac aaccccatgc aatgaccctc cgaatacagc cccaccgtcc
    76801 attccgattt tatgatcctg aatccagagg tctgttgtct gcatatctcc atggagcatt
    76861 ggtgtaatcg tatgtccaaa tacaattgtc ttacctgtat tgtttttccc ttcatgaaat
    76921 ggttccctta tccaaataaa gtctcttaga cttgtcttac gccagtcttt ctttgtcaaa
    76981 tccacaccgg catggacaaa aagataattt tcccattcat aatacaaggg gcgctggact
    77041 aaaaatttaa tcagttctga atatctcgat tggatcatca tcgagatttc agttggggag
    77101 tattcctccg atgcccctgg gtgaatcagg ctttcaaacg tttcttttcc accatttctc
    77161 atataaggcg cataccagtc ctccggtgat tgcagaaatt gcaaaaaata ttcttcatgg
    77221 tttccccgga ggtaaatggc tcctgtttct tcgactaatt gctttcctaa taaaaaacat
    77281 tcttttgtac gaggacctcg atcattgaga tcccctaata aaacaagctg atggatcttt
    77341 ggatcataat agtgaataag tttttcaaaa agctcatgtt tgccatggac atccccaata
    77401 gcataaatca atgatttcat gtggatcacc atcctcaacg ctagtatagc gcatattgga
    77461 aatggtcgac aatcaaaact attgttttct atttaattaa agctgtaaac cgcagttctg
    77521 cttcttctgg agagattcca tggtcttgaa catagcgatt gcgcggatgt ttcgcacact
    77581 ctatgctaca tgctcctaaa tgtttttctt cattttcaat ggaagcaaga atctgacgat
    77641 tgcactcagg attgctacaa ttgatatatc gttcacatgg tgttccgtca aaccagtcgc
    77701 gaccgacgat ttgtttatct acttggttga tatcgacagc gatccgttca tcaaacacat
    77761 acatcttgcc gtcccataat tctccttgga cttcaggatc cttaccataa gtagcaatac
    77821 cgccatgcag ctgtccgaca tctttgaatc cttcacggac aagccaacca gaaaacttct
    77881 cgcaacgaat gccaccagta cagtaggtca ctacccgttt ttccataaat tcttctttgt
    77941 gctcgcggat ccagtctgga agctctcgaa aactgcgtat atctgggcgt acagcccctc
    78001 gaaaatgtcc caagtcatat tcataatcat tccgtgcatc aatcaccact gtctgttcat
    78061 ctaaaatagc ttctttaaat tcttgcggtg aaagataggc acctgtcact tggtttggat
    78121 caatgtcctc ttctagcttt aaacttacaa gctctggacg gtatcttaca aacatcttct
    78181 taaaggcatg tccttcgctt ggatcgatct tgaacatcgt atcagcaaat cgaggatcag
    78241 ctttcattgc tttcatatag gcagtcgtgt tttcaacggt tccagacaca gtaccattta
    78301 ttccttcttc agctactaga atacgacctt taagatccaa agatttgcaa aaggctaaat
    78361 gatttttggc aaattcctct ggttcttcga tttttgtata attgtaataa agcagtactt
    78421 gataatccat gtatttcttc cttccttctt ttcgttccta aacagtataa agaaggcgtt
    78481 cgcatttgac gatttatttg cttgtgtata attacgcatg gaaagttttt tgtttacata
    78541 atattattat ttgaattaaa aagacttcgc tttcctgttt cgcacagaaa aacaaagtct
    78601 ttatttctat tatttacctg taaccgcaaa gtagttgcca tcgggatcag caaagttaaa
    78661 gacatattct tctccaagct gtaccatatc acctaatgtg actgattgcg tctgcatttt
    78721 tttatatagt ccaaagatat ctttactgta aaacatcaaa gatggggcac tagtagctgt
    78781 gccaggtgaa tgggactcaa caaatgctcg atcatataac agaaatgagg tttgactgtc
    78841 ctcaactcct acttcaatta taagcgtccc atccatttct tgacgatctt tttccgcaaa
    78901 gccaactgct ctccaaaacg ttgcattttt ttctacatcg tccacataaa gcatgatttt
    78961 tatttcattc gtaaacatct gtttcctcct aaacgatctg ttctaacatt ctcaactttg
    79021 tcagtttacc ataggaaaat gggaaaatct acgaaagctc tcaacagaat cgtcctactt
    79081 aaatacgctt ttctttaata ataaataagt tggttgtttt tgttatttgc gctttcactt
    79141 ttcggaataa ataatgtgta tttatatgga ataaataaaa aaagaggtcg tgactaaaaa
    79201 caagtcacaa cctctggtaa acggtgttct cctgacaact cctcggtcac aagacacaat
    79261 attcaacaaa catgagaagc tattttttga aattgctctt tgtgactcag agtgaaacgg
    79321 caggctcacg acctctttct
//
LOCUS       DACEDO010000005        72660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEDO010000005 DACEDO010000000
VERSION     DACEDO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.005"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            100..570
                     /locus_tag="IWO52_000358"
     CDS             100..570
                     /locus_tag="IWO52_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0354652.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IWO52_000359"
     CDS             792..1895
                     /locus_tag="IWO52_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0354653.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2116..3075
                     /locus_tag="IWO52_000360"
     CDS             2116..3075
                     /locus_tag="IWO52_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0354654.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3245..3925
                     /locus_tag="IWO52_000361"
     CDS             3245..3925
                     /locus_tag="IWO52_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0354655.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            4015..5049
                     /locus_tag="IWO52_000362"
     CDS             4015..5049
                     /locus_tag="IWO52_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0354656.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            5063..5398
                     /locus_tag="IWO52_000363"
     CDS             5063..5398
                     /locus_tag="IWO52_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354657.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            5382..7862
                     /locus_tag="IWO52_000364"
     CDS             5382..7862
                     /locus_tag="IWO52_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354658.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7918..9477
                     /locus_tag="IWO52_000365"
     CDS             7918..9477
                     /locus_tag="IWO52_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0354659.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            9474..10838
                     /locus_tag="IWO52_000366"
     CDS             9474..10838
                     /locus_tag="IWO52_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0354660.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            11009..12079
                     /locus_tag="IWO52_000367"
     CDS             11009..12079
                     /locus_tag="IWO52_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0354661.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            12094..12762
                     /locus_tag="IWO52_000368"
     CDS             12094..12762
                     /locus_tag="IWO52_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354662.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12782..13360
                     /locus_tag="IWO52_000369"
     CDS             12782..13360
                     /locus_tag="IWO52_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0354663.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13761..14672
                     /locus_tag="IWO52_000370"
     CDS             13761..14672
                     /locus_tag="IWO52_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0354664.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(15050..15967)
                     /locus_tag="IWO52_000371"
     CDS             complement(15050..15967)
                     /locus_tag="IWO52_000371"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0354665.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            16169..16606
                     /gene="zapA"
                     /locus_tag="IWO52_000372"
     CDS             16169..16606
                     /gene="zapA"
                     /locus_tag="IWO52_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0354666.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            16664..17212
                     /locus_tag="IWO52_000373"
     CDS             16664..17212
                     /locus_tag="IWO52_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0354667.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            17327..19687
                     /locus_tag="IWO52_000374"
     CDS             17327..19687
                     /locus_tag="IWO52_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0354668.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            19774..20088
                     /gene="trxA"
                     /locus_tag="IWO52_000375"
     CDS             19774..20088
                     /gene="trxA"
                     /locus_tag="IWO52_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0354669.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            20270..22048
                     /gene="uvrC"
                     /locus_tag="IWO52_000376"
     CDS             20270..22048
                     /gene="uvrC"
                     /locus_tag="IWO52_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0354670.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            22193..23092
                     /locus_tag="IWO52_000377"
     CDS             22193..23092
                     /locus_tag="IWO52_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354671.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            23085..24347
                     /locus_tag="IWO52_000378"
     CDS             23085..24347
                     /locus_tag="IWO52_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0354672.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            24508..26406
                     /locus_tag="IWO52_000379"
     CDS             24508..26406
                     /locus_tag="IWO52_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0354673.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(26736..27455)
                     /locus_tag="IWO52_000380"
     CDS             complement(26736..27455)
                     /locus_tag="IWO52_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0354674.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            27792..28124
                     /locus_tag="IWO52_000381"
     CDS             27792..28124
                     /locus_tag="IWO52_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354675.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            28111..30114
                     /locus_tag="IWO52_000382"
     CDS             28111..30114
                     /locus_tag="IWO52_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0354676.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            30133..30603
                     /locus_tag="IWO52_000383"
     CDS             30133..30603
                     /locus_tag="IWO52_000383"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0354677.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            30624..31208
                     /locus_tag="IWO52_000384"
     CDS             30624..31208
                     /locus_tag="IWO52_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0354678.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            31223..32209
                     /locus_tag="IWO52_000385"
     CDS             31223..32209
                     /locus_tag="IWO52_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0354679.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            32199..32510
                     /locus_tag="IWO52_000386"
     CDS             32199..32510
                     /locus_tag="IWO52_000386"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0354680.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            32532..34313
                     /locus_tag="IWO52_000387"
     CDS             32532..34313
                     /locus_tag="IWO52_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0354681.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            34306..35682
                     /locus_tag="IWO52_000388"
     CDS             34306..35682
                     /locus_tag="IWO52_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0354682.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            35689..36324
                     /locus_tag="IWO52_000389"
     CDS             35689..36324
                     /locus_tag="IWO52_000389"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0354683.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            36354..37709
                     /locus_tag="IWO52_000390"
     CDS             36354..37709
                     /locus_tag="IWO52_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0354684.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(37901..39295)
                     /locus_tag="IWO52_000391"
     CDS             complement(37901..39295)
                     /locus_tag="IWO52_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0354685.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            39387..40358
                     /locus_tag="IWO52_000392"
     CDS             39387..40358
                     /locus_tag="IWO52_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0354686.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            40355..41137
                     /locus_tag="IWO52_000393"
     CDS             40355..41137
                     /locus_tag="IWO52_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0354687.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(41306..41677)
                     /locus_tag="IWO52_000394"
     CDS             complement(41306..41677)
                     /locus_tag="IWO52_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0354688.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            41811..42254
                     /locus_tag="IWO52_000395"
     CDS             41811..42254
                     /locus_tag="IWO52_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0354689.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(42297..43106)
                     /gene="yidA"
                     /locus_tag="IWO52_000396"
     CDS             complement(42297..43106)
                     /gene="yidA"
                     /locus_tag="IWO52_000396"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0354690.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(43125..44492)
                     /locus_tag="IWO52_000397"
     CDS             complement(43125..44492)
                     /locus_tag="IWO52_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0354691.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(44476..45306)
                     /locus_tag="IWO52_000398"
     CDS             complement(44476..45306)
                     /locus_tag="IWO52_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0354692.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            45469..45906
                     /locus_tag="IWO52_000399"
     CDS             45469..45906
                     /locus_tag="IWO52_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0354693.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            45982..46602
                     /locus_tag="IWO52_000400"
     CDS             45982..46602
                     /locus_tag="IWO52_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0354694.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            46791..47297
                     /locus_tag="IWO52_000401"
     CDS             46791..47297
                     /locus_tag="IWO52_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0354695.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            47314..48213
                     /locus_tag="IWO52_000402"
     CDS             47314..48213
                     /locus_tag="IWO52_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0354696.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            48300..49184
                     /locus_tag="IWO52_000403"
     CDS             48300..49184
                     /locus_tag="IWO52_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0354697.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(49376..49447)
                     /locus_tag="IWO52_000404"
     tRNA            complement(49376..49447)
                     /locus_tag="IWO52_000404"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49412..49414),aa:Arg,seq:ccg)
     gene            49507..50841
                     /gene="rpoN"
                     /locus_tag="IWO52_000405"
     CDS             49507..50841
                     /gene="rpoN"
                     /locus_tag="IWO52_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0354698.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            51044..52081
                     /locus_tag="IWO52_000406"
     CDS             51044..52081
                     /locus_tag="IWO52_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0354699.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            52122..53123
                     /gene="gap"
                     /locus_tag="IWO52_000407"
     CDS             52122..53123
                     /gene="gap"
                     /locus_tag="IWO52_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0354700.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            53233..54423
                     /locus_tag="IWO52_000408"
     CDS             53233..54423
                     /locus_tag="IWO52_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0354701.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            54527..55282
                     /locus_tag="IWO52_000409"
     CDS             54527..55282
                     /locus_tag="IWO52_000409"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0354702.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            55398..56696
                     /gene="eno"
                     /locus_tag="IWO52_000410"
     CDS             55398..56696
                     /gene="eno"
                     /locus_tag="IWO52_000410"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0354703.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            57096..58235
                     /locus_tag="IWO52_000411"
     CDS             57096..58235
                     /locus_tag="IWO52_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0354704.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(58302..58877)
                     /locus_tag="IWO52_000412"
     CDS             complement(58302..58877)
                     /locus_tag="IWO52_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0354705.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(58882..59856)
                     /locus_tag="IWO52_000413"
     CDS             complement(58882..59856)
                     /locus_tag="IWO52_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0354706.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(59880..59924)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            60139..61107
                     /locus_tag="IWO52_000414"
     CDS             60139..61107
                     /locus_tag="IWO52_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0354707.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            61220..61498
                     /locus_tag="IWO52_000415"
     CDS             61220..61498
                     /locus_tag="IWO52_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0354708.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            61616..61852
                     /gene="secG"
                     /locus_tag="IWO52_000416"
     CDS             61616..61852
                     /gene="secG"
                     /locus_tag="IWO52_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0354709.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            61964..62710
                     /locus_tag="IWO52_000417"
     CDS             61964..62710
                     /locus_tag="IWO52_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0354710.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            62713..65070
                     /gene="rnr"
                     /locus_tag="IWO52_000418"
     CDS             62713..65070
                     /gene="rnr"
                     /locus_tag="IWO52_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0354711.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            65092..65556
                     /gene="smpB"
                     /locus_tag="IWO52_000419"
     CDS             65092..65556
                     /gene="smpB"
                     /locus_tag="IWO52_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0354712.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            65858..66577
                     /gene="atpB"
                     /locus_tag="IWO52_000420"
     CDS             65858..66577
                     /gene="atpB"
                     /locus_tag="IWO52_000420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0354713.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            66630..66845
                     /gene="atpE"
                     /locus_tag="IWO52_000421"
     CDS             66630..66845
                     /gene="atpE"
                     /locus_tag="IWO52_000421"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0354714.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            66936..67460
                     /gene="atpF"
                     /locus_tag="IWO52_000422"
     CDS             66936..67460
                     /gene="atpF"
                     /locus_tag="IWO52_000422"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0354715.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            67447..67989
                     /locus_tag="IWO52_000423"
     CDS             67447..67989
                     /locus_tag="IWO52_000423"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0354716.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            68012..69568
                     /locus_tag="IWO52_000424"
     CDS             68012..69568
                     /locus_tag="IWO52_000424"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0354717.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            69582..70484
                     /locus_tag="IWO52_000425"
     CDS             69582..70484
                     /locus_tag="IWO52_000425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0354718.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            70507..71913
                     /gene="atpD"
                     /locus_tag="IWO52_000426"
     CDS             70507..71913
                     /gene="atpD"
                     /locus_tag="IWO52_000426"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0354719.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            71927..72349
                     /locus_tag="IWO52_000427"
     CDS             71927..72349
                     /locus_tag="IWO52_000427"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0354720.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        24161 a        12722 c        15430 g        20347 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact tcgccgattg taaaattaag
     2041 ctagacaact aaaaaaagtc atttgtgcta cactcaaaat agttccgacg aaagaattat
     2101 aaggagtgag tacaaatgac ctataaccat cttacaccaa ctgaactcgt aatgatagaa
     2161 gcttatttca atcaatcaca acctgtttca aaagtagcca atctgttaca acgttctcga
     2221 caaactattt ataaggtcta tcgttttctt aaatctggtg gaagcgcaat gagctactat
     2281 aaacaatata agaaaaacaa acgtaactgc ggaagacatc cgattgtttt acctgatgag
     2341 caacaggaat acattcaaaa aaaggttgtt caagggtggg ctcctgatgt ccttattggt
     2401 cgagcagagt ttcctattgc ttgttcggta cgcacactgt atcgaatgtt taaagataaa
     2461 acctttgaca ctcacgattt accaatgaag ggaaaaagaa aaccaaacgg tcataaagaa
     2521 aaaagaggaa aacaagggtt tcgccggtca atccgagaca gaaagacaga ctatacccag
     2581 tttgatcatg aatttggtca cctggaaggt gacactatcg tgggtggaaa acataaaagc
     2641 gcggtcatca cattagttga acggctatct aaagtcatca ttaccttgaa acccgaaggc
     2701 agacaggcta aagacattga aaataggctg aatcaatggt ttcgttgtgt cccaaaaaac
     2761 ttatttaaat ccatcacatt tgattgtgga aaagaattct caaattggaa aaagattagt
     2821 aataccaacg atatttcaat ttattttgcg gatccaggaa caccttctca acgaggattg
     2881 aatgaacatt caaatggctt attacgtaaa gatggtttgt ataaagctat ggattttaat
     2941 ggtgtatctg aaacctttat ccaatcggtg gcatcgaaac gaaatcatat tcctagaaaa
     3001 tcattacact atcggacacc aatggaagta tttttgagtt acgtcacaaa agatgatttg
     3061 tctaacttaa tttgacaaac gaaacttaat aaaaaaggaa gaatattcat gtaaacgatt
     3121 ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc
     3181 atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt
     3241 tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc taggcggctt
     3301 ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga
     3361 accaacaaat accgtggagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc
     3421 agatccggaa aatccagcaa ctggccagac aggtccttta acactggatg tagttcccga
     3481 attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc
     3541 aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca
     3601 agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt
     3661 gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc
     3721 cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat
     3781 atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa
     3841 tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa
     3901 cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat
     3961 actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg gacaatgaat
     4021 caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg
     4081 tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag
     4141 aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag
     4201 acactagaag taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc
     4261 aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta
     4321 gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat
     4381 gtatcagcac atgcagaaac aactgtacct atagaagtga aaatcccagc aaaaagcttt
     4441 aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat
     4501 gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa
     4561 gaaagtcaag agacgataga accggatctg aaacttttat caggagattt ggacgaggtc
     4621 aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat
     4681 ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat
     4741 gcatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa
     4801 gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg
     4861 cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc
     4921 gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt
     4981 ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa
     5041 acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag
     5101 tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat
     5161 tacattttat gatctaagtc caattccgca tccaagtggt tcagtcaatg gcgcatcaaa
     5221 acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac
     5281 gaatgaacaa aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc
     5341 gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat
     5401 tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt
     5461 caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt
     5521 atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc
     5581 aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa
     5641 atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa
     5701 atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt
     5761 tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact
     5821 tgtatattgc cggtgccccc atcaaggtta ctagcggaag tttattatca gcaggaagcc
     5881 ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattgaca gatattattg
     5941 ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag
     6001 ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt
     6061 tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt
     6121 atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag
     6181 gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg
     6241 caagtgcaga gataaatacg tggaatagtt acatctcacc aagtgattca acaggaaaat
     6301 ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct
     6361 ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat
     6421 ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa
     6481 tactgactca tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca
     6541 gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt
     6601 acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc
     6661 tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg
     6721 gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag
     6781 atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac
     6841 tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta
     6901 ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc
     6961 ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc
     7021 ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag
     7081 aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga
     7141 tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta
     7201 tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag
     7261 gagataccat tcatcttact tacaactatc aattaaatac tgtagatact tctttacaat
     7321 ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg
     7381 ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag
     7441 aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt
     7501 tgaagacaag ttattatgaa tcaaaaggcg atttttcttt tgatgtgcgg gatactcggc
     7561 ttccaagcac gtctccttgg caactgacag gtacattgac ttctttattt aagaatgatc
     7621 aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca aagcaattga
     7681 ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc
     7741 tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc
     7801 aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg ccaaccagct
     7861 aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg
     7921 agaaggaaac ttctgctgtt aaaatcagaa atgcttgaag gagtggactt ggaacttttc
     7981 agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa
     8041 aaaggagagg tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg
     8101 aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt
     8161 atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat
     8221 tatcattgtt acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat
     8281 ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct
     8341 cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg
     8401 cggctgaccg gtccagaaag tcaaattcgt tatttttatt tccaattatt ttggtttgga
     8461 cgtccctatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat
     8521 ctgctgcaaa aagagtttag ttttgctatt acagaagaag gacgattgaa atttgaaata
     8581 tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc
     8641 aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg
     8701 acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat
     8761 cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg
     8821 ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg
     8881 atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg
     8941 acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc
     9001 caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt
     9061 gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat
     9121 gcccgctttg aattatttgc tcgctatgca tctattttac ggacgctgat caagcagtta
     9181 gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg
     9241 aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag
     9301 aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag
     9361 tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac
     9421 caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa
     9481 tcgtgatcat tggtgcttcc catgaaggca tcacagctgc cctaaatcta agaaaacttc
     9541 aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt
     9601 caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata
     9661 acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac
     9721 ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa
     9781 cctatgataa attaatcgta gcgacaggtt cctctcctgt caccttgtat aaacaaattg
     9841 aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt
     9901 taaaagaagc aaaagaagta gtgatcttcg gtgctggtta cattggattg gaattagcag
     9961 atgctttgag aaataaagga tacattattc atctagttga ttatatgccg aatgttttat
    10021 caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa
    10081 taaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat
    10141 ccgtacaact tctgtctcaa acaataaagg cagatatggt cattttttct gcccaaactc
    10201 gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag
    10261 tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag
    10321 tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata
    10381 tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa
    10441 agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg
    10501 aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac
    10561 aatacgacga agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag
    10621 aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg
    10681 cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc
    10741 tgtctgaata cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta
    10801 agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg
    10861 tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg
    10921 gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa ttgttgtata
    10981 aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa ttatgcattc
    11041 aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc tggtattttt
    11101 tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg acaagtggga
    11161 ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt ccatgattac
    11221 taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc aaacacctgg
    11281 agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct tcttcggtat
    11341 cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg ataacgatga
    11401 tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccatag gagatacagt
    11461 taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc atgccaaatt
    11521 caatgtctca ccagttctct atacaaccat caatgcttac caagaaatcc gcttcgaaaa
    11581 agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta aaatcagtaa
    11641 tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat tacctggata
    11701 taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta ttgcggctat
    11761 tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct ttggtgtaat
    11821 gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac aaacattcat
    11881 tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat cccttgttct
    11941 acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta gcttgttgat
    12001 gttagtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa aaatcgatcc
    12061 gttgaaggca attggataag aaggaggaag aacatgaaag caatagaatt taaagaagta
    12121 gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac aaccatgagt
    12181 atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa aagtactttt
    12241 ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat caatgatcaa
    12301 gaattcagta atcaaaaaga aagcgaacgc gttaaactac gttttaaaga aattggcttt
    12361 attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt attattagta
    12421 gataagatta aacatgaaca tcgtcaaaaa gaagcaaacg aattatttga gcagcttggc
    12481 gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg gcaaagggtc
    12541 gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga accaactgct
    12601 agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga atcaaaagac
    12661 aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa atgcgataga
    12721 atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag caggtggaaa
    12781 aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag cgactgactt
    12841 gtttatgtcc aagggatatc tcggcacttc aacaagagaa atcgcacaaa aagttgggat
    12901 cacccagccc aatctctatc attatttcgg tgataaagaa aaactatata cggcagtttt
    12961 agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa caagcgaaac
    13021 gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac atctggtcga
    13081 tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa gagatcatct
    13141 tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt cagcaaacca
    13201 atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt tcttagtgtt
    13261 agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg tggcgcaatt
    13321 gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata tagggagaaa
    13381 atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgcttc tttatatagg
    13441 tagtttttca taaatacaaa tttgttattc tctcatgatt atcagggtat atttttattt
    13501 attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa ttgtttgtag
    13561 aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa ggagatttca
    13621 ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta cggttatatc
    13681 ttttacagcc ccttttttgc ttacttaagc tgattttaag ttttggccct tagtctggat
    13741 ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt gcaatcatta
    13801 tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg
    13861 tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat acagaacaag
    13921 agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac tcgctaacta
    13981 ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa acaaaaaatg
    14041 cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa gatggaacca
    14101 aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg attgtattag
    14161 atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa acagaagaaa
    14221 aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat gctggagcaa
    14281 cggtttattt gacccatact acagatgaat tagtacagct tggagatata tgcgactaca
    14341 gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg gaatatgcga
    14401 atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca ttagcacaga
    14461 caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc actgggaact
    14521 atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt tatatgaata
    14581 atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt gctgcaagtt
    14641 tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa caaaaatagc
    14701 tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt aaacaccgtt
    14761 taaggaagag gtcgtgagcc tgccgttcaa ctctgagtca caaggagcaa tttcaaaaaa
    14821 cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca tgagaacacc
    14881 gtttaagaaa aaggttgtga aatcaacgtt ttgttctgag tattagagaa acaaacggaa
    14941 aaatagttcc tcatattttg ccgtttgttg agctaatacc gaggatcaga tgattgaaca
    15001 ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat tattttttta
    15061 gtagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt tcaagcatag
    15121 gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt ccagctcctg
    15181 acggaagggt gattccagct tcagctgatg ctttgtctaa ctcttccaaa aagctggcac
    15241 gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta gtaacgaagt
    15301 acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa tttgcttccg
    15361 gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt tttagcagca
    15421 aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat tttggttgga
    15481 tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt ttttttaaga
    15541 tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta acaccaagtt
    15601 tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt agcggaccga
    15661 aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg atattttttg
    15721 gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc ccccatttat
    15781 cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta taagcggtaa
    15841 tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta tttaacaaat
    15901 aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg ataatgacgt
    15961 tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca cgacttttcg
    16021 ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa tagttaagta
    16081 tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac taaataaaac
    16141 tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct acaaagcggt
    16201 gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata tggatatcgt
    16261 aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac aaatggataa
    16321 tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga agaaacaaga
    16381 aagaatattg gagctggaag aagaaacagc agaattaaag aaaaaaatga tcaaatttac
    16441 agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc gggaagtatt
    16501 gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac aacagatttt
    16561 aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa
    16621 aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
    16681 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
    16741 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
    16801 agcaagtcat ttggaattat acattccata cccagctgtc accccaacat caaagttagc
    16861 attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag gaactgcttt
    16921 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
    16981 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
    17041 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
    17101 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc
    17161 atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata
    17221 gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc tgcgtagcgt
    17281 atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat
    17341 ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc
    17401 acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa cgatcacaaa
    17461 ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat
    17521 acctaaaatt gaaaatatcc gtccccatat gaaacggatt gagatcggag cagacttgaa
    17581 tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt
    17641 cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca
    17701 gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg
    17761 cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga
    17821 acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag
    17881 tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg
    17941 cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggtcagactt tatttattga
    18001 accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa
    18061 tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat
    18121 catccacaat acttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg
    18181 taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca
    18241 agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa
    18301 agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa
    18361 gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga
    18421 aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga
    18481 gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt
    18541 caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg
    18601 tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc
    18661 aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata cgattaatgc
    18721 cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga ttggcgttcc
    18781 tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga
    18841 tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt
    18901 agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga
    18961 ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga
    19021 aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg cggaagaaaa
    19081 agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt
    19141 gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac
    19201 tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga
    19261 cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca
    19321 gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt
    19381 tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag
    19441 tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt
    19501 agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg
    19561 aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt
    19621 caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt
    19681 taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac
    19741 tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca
    19801 tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt
    19861 ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa
    19921 ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc
    19981 atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt
    20041 gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca
    20101 aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt
    20161 aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt
    20221 tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg
    20281 aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa
    20341 aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata
    20401 ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga
    20461 atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa
    20521 aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat
    20581 aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag acaaagcact
    20641 gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg
    20701 attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca
    20761 tttgggacaa tgcctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat
    20821 cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt cagagatcca
    20881 tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga
    20941 ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact
    21001 ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc aagtgttttt
    21061 cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc
    21121 agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc
    21181 taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac
    21241 gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgagtaagaa
    21301 tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat
    21361 cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc
    21421 ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga
    21481 tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga
    21541 tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga ttcgcgaagg
    21601 gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa
    21661 agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg taaaaaacga
    21721 taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac cgcttgagcg
    21781 aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat
    21841 cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga
    21901 agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat
    21961 cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa
    22021 cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt
    22081 cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc
    22141 ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga
    22201 agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca
    22261 aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac
    22321 ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca
    22381 tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta
    22441 tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag
    22501 aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa aagggaaaaa
    22561 gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac
    22621 gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt
    22681 aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat
    22741 tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg
    22801 aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac
    22861 gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa
    22921 aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg
    22981 gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc
    23041 gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga
    23101 ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat
    23161 tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata
    23221 gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc
    23281 aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag
    23341 agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa
    23401 atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac
    23461 aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca ccagaagaag
    23521 tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag
    23581 gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt tatgtagcaa
    23641 cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt
    23701 ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt
    23761 tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta
    23821 ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag
    23881 acggtatctc attggataac ttgattggac cattcttgtg gtattctctt ctattcatga
    23941 tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag
    24001 cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc ggatacatgc
    24061 ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata
    24121 ttccattctt atcttcatat attatgccga ttcgcttagc aaatgagaca gtcggtatgg
    24181 gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt
    24241 ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta tggagttcat
    24301 tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac aatcctaaaa
    24361 tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac ctattttatt
    24421 tattttacta tattggatat atattcattg aaaatagtgt ataacaactt tataatattg
    24481 agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact ttcagaaaaa
    24541 ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    24601 ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc ttttgatcat
    24661 attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt ttctgaacaa
    24721 atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga atatgcatta
    24781 acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt gatcgatctt
    24841 ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt gccgaaagaa
    24901 tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg aaaaaaagac
    24961 tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg aagaagaaaa atttttaacg
    25021 aaacttgcac agacgatcca aaaatctgtc atcgatcatt tgcatatcgt tggagatatt
    25081 tttgaccgag gaactcaagc agccaaagtg atggatcgaa tcatggattt ttcttctgta
    25141 gatatccaat ggggaaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    25201 tgtttactga cacttttacg gattgcagca cgctataatt atttatttga attagagaaa
    25261 gaatatgggt tgaatttacg tccattgttt tcttttgctc atcaaactta tcagaagaat
    25321 ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa gagagcaaga agaattagaa
    25381 aaaatccatc aggcactttc tatccttcaa ttcaaatcag aacatcagtt attggatcgt
    25441 cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa aaatcgacta tgaagcatca
    25501 acgattgatt tagaaggtca gctctatgag ttgaaaggaa cttgttttca aacgatccag
    25561 ccagatgatc caaaaaaatt cttggaagag gaacaaaaag tgattgatag tttgatgtat
    25621 tcattccagc actctttgag aatgcaaaaa catatgacgt ttttgatcga aaaaggggga
    25681 atgtatctca cgaacaatca gcatgtgctg tttcatggct gtatacctgt agacgagaag
    25741 ggacagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggcttt
    25801 tttgaaaaac agataactga aagcgcaaaa gatctgactg caaaagaaga tttagcaacc
    25861 gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt tattcggcaa gagtaaaatg
    25921 gcgacttttg aacgttattt tctcacagag cctcatacgc acgtcgagaa aagcaatcct
    25981 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttagcaga attcgacaca
    26041 aaagaagaag ggtcagcaat cattaatggg cacacgccag taaaagtaaa aaaaggggag
    26101 tcaccgatca aagcaaatgg aaaagtattt gtcattgacg gaggtttatc caaagcttat
    26161 caaaaaacga caggaattgc cggttattca ctattatgca attcttatgg tttccaaatc
    26221 gtgacacatt atccgttttt gcctattgaa caacaatttg caaaaaaatc cgaccaaaca
    26281 aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac tcaatcgtga tacaacaaaa
    26341 ggtatggaac tgcagcagca gattacccag ctcaaaaggc tgttggacta tcgaaaggac
    26401 gattgagaag aattccagtg aacattgttc catagactgg agactgagac aaaagtcgtg
    26461 tcgcagtctc tttttccgga taaacggtgt tcttctgcca actcctcggt cacaagtcac
    26521 actattcaac aaacatgaaa agctgttttt tgaaattgct ccttgtgact cagagttgaa
    26581 cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gccagtttct cggtattaag
    26641 ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa tttttcttaa tactcgaaac
    26701 taaacagctt tttcacaacc ccctcagaaa gtctattaaa attcattcac aaagaaatcg
    26761 aataagcgct gttcagatgg atgtacacgg tgggtaagtt ccggatgcca ttgaacaccg
    26821 aggattttat gcatatcatc tttgctttga accgcttcaa cgagtccatc gttagaaaaa
    26881 gcgatggctt ttaataaagg tgaaagatct ttgactgcct gatgatggta agaattcaca
    26941 aaatatggat cggggagaag gttgcttaac aaactgtctg aaacaatctt gacttcgtga
    27001 gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta ctgaccactt tggatataat
    27061 gaaagatctt gataaagtgt accttccaaa acgacattca atagttggag tccccggcag
    27121 actgcaaaaa ttggttttct ttgtttcaat gcttccaaga tcagtgcctg ttcaaattgg
    27181 tctcgttgaa tatttgtttc cgttagcttt ggatgaggat cttccatgta aagttgtgga
    27241 ctgacatctt gaccacctgc aagaataagt ttgtcgattt gtccaatcag ttgaggagct
    27301 gaatctggag acatgactgg cagaataaga ggcaatcctt gagcatctaa aactgcatcc
    27361 acgaatcctt gcggtgtata gctcacttga tttccttgga aagtatctgt tgctcgaatc
    27421 agttgatttc cggcaatgcc gatgattggt ttcatttgta ttccccctaa agtagactta
    27481 ttgtaacaag gatttaaaaa aaattaaact atgtaagact tgcccctata cttatctgtt
    27541 cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa attcatgacg atagttgaaa
    27601 atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg gcgtaagctt
    27661 caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc tgcctatact
    27721 aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc tcttttttaa
    27781 atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa gcagaagaag
    27841 aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa gatctatgca acagaaaaag
    27901 agcaggaatt ggaaacgatc caaacaaact atcgacaaca actaaaacag ttggtggaag
    27961 aaaaagaaca atttgaaata caagaattag aaaaagaaaa aatgaaattg aaagcagaag
    28021 cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa gcatcagagt caagcgatcg
    28081 gtgcgatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga aaaagtgacc
    28141 ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg aatccaaaac
    28201 gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtgga gaagtatttt
    28261 ccagatacca cgattattga taaagaagca aaattaagcg ctttaggtaa tcggttgaaa
    28321 gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca aaagaaactc
    28381 catttgaaac gtcaagaact ttccttacaa gatttggaag ccaattattc cgaagaaaat
    28441 ttcataaata aattgaaaga agtccttgat ttaaaagaaa agtgggaaca actaactgaa
    28501 gaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca gctggatatt
    28561 gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt gagtgcagca
    28621 aattttgaaa gtttcaagga agaactgagt aagcttgatg acgtttatat ggaagaaata
    28681 gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc agttgctgtt
    28741 atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta tgaacaaaat
    28801 cctaaagaaa aactgcaaga gaccaagcag caattatcca ttgttcacga gcagcaaaag
    28861 aagataacga cagcgttagg tcagtgcagt ggctatatcc aagaatttga atgggtagaa
    28921 gaagtcacac ttgccgttgc agaacgagaa aaaataaaag aacggtttat ccatgcgact
    28981 tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt gctgcatgtg
    29041 ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt tcgaaacacc aaactcagaa
    29101 gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttgtcga accttttgaa
    29161 ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac accttggatg
    29221 acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta tggtttgctg
    29281 atgctcattg ggacaatact agctcaaaaa ctacttgttc taccacgcgg gatgaaacgt
    29341 ttcgtaaaat tctttgagat tctctcgata ccatcgatca tctggggatt gatctacagt
    29401 tcatttttcg gacagtcatt accaaaagaa ctgtttggca tccgcttgcc tttcccaatc
    29461 ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt cggtctgata
    29521 cagattttgg ttggattatt tgtggcagca aaggaaaata tcaggagaaa agattacttg
    29581 agtgctgtca gtgatggatt tgcctggcaa gggatcctaa ttgggatcgt tattgcgttg
    29641 gttggtgcca tgttgataaa aaatacagca tttgtttacc ttggcgctgg gctatcgatc
    29701 attgctgcct tatgtatctt gatcgttccg attatccaaa cgccgtctaa agtcaaaggt
    29761 gctgccaaag gtgcgtataa tctttacgga ctgaccggct atatcggtga tttagtcagt
    29821 tatacacgtt tgatggcatt gggtatttca ggcggaagta ttggagcggc attcaatatg
    29881 ctagttgctt tcatgccgcc agctgctcga ttcagtgtag ggatcctttt gatcatcgct
    29941 ttacacgcac tgaatatgtt tttgacactg ctaagtgcct atgtccatgg cgcacgattg
    30001 cagtatgtcg aattttttgg gaagttttat acaggaggcg gacgtgcctt tgatccgctg
    30061 aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa aaaacaataa ataattggag
    30121 gatttttata taatgatgga ttacttaatt agtcaaaatg gtggaatggt atttgcggta
    30181 ttagcgatgg caacagcgac gattttttca gggatcggtt cagctaaagg tgttggaatg
    30241 acaggtgagg cagcagcagc attgacaaca agtcaacccg aaaaattcgg gcaggctctg
    30301 attctgcaat tgcttccagg cacgcaaggt ttgtatggat tcgttattgc ctttttgatt
    30361 tttatcaact taggaaatga catgtcagtt gtacaaggac tgaactttct aggtgcttct
    30421 ctgccgattg cttttacagg cttgttttca ggtattgccc aaggaaaagt cgcagcggca
    30481 ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat ttttgctgcg
    30541 atggttgaaa catacgcgat tttaggtttc gttatttcct tcctattagt gttgaatgcc
    30601 taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct cagatgaacg
    30661 aagcagcaga gcaggaaaga gcggcgttag aacaagaaga gcgaatgaag atcgatcaaa
    30721 actttgaaca aaaacgtacg caagttgaaa cagaacatca aaaacaaaaa gaaaaacaaa
    30781 tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg ccagcaagta gaagtacgac
    30841 aagaaaattt gaatgcgaag caggaatttt taagacgatt gtttgcagat gcggtaactg
    30901 aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat caaaaatgca ttgtattcat
    30961 tacctttgac aggaaaagta gcattcattg ctggagaaaa atcagcagcc tatctttctc
    31021 agacgttgtt ggatgaatgg aacaatgaac ttccctttat gatggtttta agtgatgaaa
    31081 ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat aattttcttt
    31141 tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc aatcaattat
    31201 tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga ttcgcggaag
    31261 agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa attctgtcga
    31321 agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg acggatatga
    31381 atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct ggttaaagga
    31441 atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgttatactt tccataatct
    31501 gaaggttttg acaaaagctg agatgacaaa tcaaaatctg gaccatttgt atatcgacga
    31561 tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt caaatgaact
    31621 tcctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag agtctaccat
    31681 tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc gacggcttgc
    31741 cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg acttgaccaa
    31801 tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca tgacgacggt
    31861 actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag ataaagatat
    31921 ggaagattac acgaattttc tattagagac agattatgct gattttctgc gccctgtcat
    31981 ccgtgaggga aatattgatc tcgtaaagct tgaacagttg aaagatgatt atctaagttc
    32041 attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag cctttttgaa
    32101 cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa gaagccattt
    32161 cgaatcagaa acaatcagaa agcgggtgag acaagtctat gacgcataag atcggtgtag
    32221 ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt cgttatggta
    32281 caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag tatggcgtga
    32341 tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt tacaaggaac
    32401 aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg atagggctgg
    32461 aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg
    32521 agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt gtaatggctg
    32581 agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta ggagttatcg
    32641 gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa gagacttctg
    32701 gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt gaattaggac
    32761 cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc tttatggaaa
    32821 tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac catgagaaaa
    32881 aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga gatgttatcg
    32941 ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat gggataaaag
    33001 gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta tgtgtcattg
    33061 aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta agacgtagtc
    33121 ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag cgagtgattg
    33181 atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct tttggtgcag
    33241 ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg gttgtctatg
    33301 tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc cctgaattga
    33361 ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct aatacatcaa
    33421 acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt gcggaatatt
    33481 tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga tgggcagaag
    33541 cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt tatccagctt
    33601 atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt gcattaggat
    33661 cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct ggaggggata
    33721 tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg ggtcttgatt
    33781 ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc tattctcttt
    33841 attcaacaga agtagggaag tatatggatc atatccttca agctgactgg gcatccatgg
    33901 tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg
    33961 tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca aaatccattc
    34021 gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact tcaagagaga
    34081 aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc cgtgcgttgt
    34141 cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt gaaaggatcg
    34201 gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt attggtgaaa
    34261 tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat taaagaatat
    34321 cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc tggtgtcaaa
    34381 tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg
    34441 gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg tatcaattta
    34501 cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc ggaagacatg
    34561 atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga gatcctgcct
    34621 gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga ttatccagat
    34681 gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt tcgtgggcaa
    34741 aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc tcagatcgcg
    34801 cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc tgctattgga
    34861 atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg cgcaatcgac
    34921 cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat cgctactcct
    34981 cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca tgttttggtt
    35041 atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc agcacggcga
    35101 gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc tactttattc
    35161 gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc gattctaaca
    35221 atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat tacagaagga
    35281 cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat tgatgtactc
    35341 ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag agaagaccat
    35401 gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta
    35461 gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc gaagtttgct
    35521 gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc gattactgaa
    35581 acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt aaaacgaatc
    35641 aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat ggcacgattg
    35701 aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac aactgccaca
    35761 agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt catcctgttg
    35821 attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga agcaatgtct
    35881 gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt atttgttttg
    35941 ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt aaaagtacct
    36001 gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata cgggtatttg
    36061 aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct tccaaaactt
    36121 ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca
    36181 cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga aacgatctat
    36241 tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt gatcaaagta
    36301 aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa acgatgatta
    36361 aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca ttaggatttt
    36421 ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt aatagtgggc
    36481 atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat
    36541 tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg atcatgcttt
    36601 tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt gcattgataa
    36661 agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat ctagaagaag
    36721 tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtg atccaattga
    36781 ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg aaaggtatct
    36841 ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat ttgttaggag
    36901 acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct gctttgatca
    36961 tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat cagaaaaaga
    37021 agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta ttaggcggat
    37081 tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg aatatgtttc
    37141 aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga tattattcag
    37201 ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg atgtacatcg
    37261 gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt gtgttgctga
    37321 tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc cgtaccatcc
    37381 gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg ttgtgtgtag
    37441 tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc atcgaatata
    37501 ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga ttgacgccag
    37561 acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga cgagttggtg
    37621 tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca aaatacaaat
    37681 atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc tagtgattta
    37741 gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt gcaaaatgtt
    37801 cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct tttttattga
    37861 aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca tattttttta
    37921 ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta gaggataggg
    37981 aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa gcgtctggaa tgataaagtc
    38041 ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca ggatcaactg
    38101 gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa agagaatcaa
    38161 aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta tttgcttcat
    38221 ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc acatttatat
    38281 taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga taagcaactc
    38341 gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg ttaggtaata
    38401 aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat gaatggatat
    38461 catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc tcccgtcttt
    38521 tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag acaccatgaa
    38581 tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat
    38641 caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta gcgtatgtat
    38701 atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg atagccaatc
    38761 cattttgaac accggaaaca atagatagag attctaagga cggaatgact ccttgagttt
    38821 ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct tggtaattaa
    38881 gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca acgaatttat
    38941 tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt acttcattgg
    39001 tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt ccaatattcg
    39061 tatacagcaa acctttctca attccatatt tataagcctt gccaacggta gtaaaattta
    39121 aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc tgtaatttat
    39181 tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga cgcttcaatt
    39241 tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata ggcattttct
    39301 ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat tttagaagtt
    39361 aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa gtagtagctt
    39421 cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg
    39481 gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa ggcaaaaata
    39541 tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca aaagtcatta
    39601 ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag attttcttgt
    39661 ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc acctggattc
    39721 gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat gcagaaacac
    39781 ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt tctaccattc
    39841 gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat acttttatcg
    39901 ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca gtagaagatg
    39961 cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca tttcttcaaa
    40021 aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact cctgatacaa
    40081 gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat tactcagctg
    40141 ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta gaggaatctt
    40201 gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat aaaggagaat
    40261 cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt tgtgcagaat
    40321 atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt acgtagttga
    40381 tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg
    40441 gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg aaacagcatt
    40501 tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc gagaaatcga
    40561 tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt acaaaatacc
    40621 tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa gaattaaaga
    40681 ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat tgtctgaata
    40741 tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt tatttttcgt
    40801 attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta tcaaaaattt
    40861 tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag
    40921 aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa
    40981 tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc
    41041 atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa
    41101 agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat
    41161 aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag acgcaaactt
    41221 tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa
    41281 tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag
    41341 ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag tcagtaatct
    41401 caagaccagc agctttatgt tgctcgtgta atttttctag atcatcagct gctatagcga
    41461 tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta
    41521 attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat
    41581 cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa
    41641 gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc cttttccttt
    41701 tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt catgagaaat
    41761 ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc atggataaac
    41821 caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca gcatatattg
    41881 tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct ttttttcttc
    41941 ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag
    42001 aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt
    42061 actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt
    42121 ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag
    42181 aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa
    42241 aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac
    42301 agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca
    42361 tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat
    42421 tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata
    42481 ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact
    42541 tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa
    42601 cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc
    42661 atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct
    42721 tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc
    42781 gaatggagat gactgccgac acggcgtgcc attacttcga tctctaggaa atcttcatgt
    42841 gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta
    42901 taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca
    42961 cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca
    43021 atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg
    43081 tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc
    43141 aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa
    43201 tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt
    43261 tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg
    43321 tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca
    43381 taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata tccgaccttt
    43441 tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca
    43501 gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca
    43561 gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct
    43621 tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg
    43681 ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc
    43741 ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata
    43801 taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga
    43861 atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca
    43921 tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact
    43981 tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct
    44041 actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg
    44101 gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca
    44161 tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg
    44221 cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca
    44281 taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa
    44341 gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat caaatctaag
    44401 attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata
    44461 ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg
    44521 ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc
    44581 ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc
    44641 tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc
    44701 aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa
    44761 tcgcttcatg acttgcccac gtttttcctg attaccgttg acacttaagt acatcataac
    44821 agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct
    44881 taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt
    44941 tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt
    45001 ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat ctgatacgat
    45061 ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatcttcca gtgtttggat
    45121 cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata
    45181 ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt
    45241 taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg
    45301 gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat actctaaaat
    45361 caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca
    45421 aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca
    45481 caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa
    45541 gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt
    45601 tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca
    45661 gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat
    45721 aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt
    45781 acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt tcgaatcgat
    45841 tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca
    45901 gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga
    45961 ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa
    46021 ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt
    46081 tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga aattcgtgat
    46141 tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc attaggggat
    46201 aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat
    46261 ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc
    46321 tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat
    46381 ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc
    46441 gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa
    46501 gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa
    46561 gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag
    46621 aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga tttttgtcac
    46681 accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa aaattgaaac
    46741 ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga atgattttta
    46801 ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat ggactcatcg
    46861 aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat aaagaagagg
    46921 ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa aaagaaaaag
    46981 agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca ttagatagtt
    47041 ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc ccccaacagt
    47101 tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata gacagtccgg
    47161 aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa attgcgacta
    47221 tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg aatgctttac
    47281 gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga aaatttaaaa
    47341 aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga ggagatccgc
    47401 cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa gagacgtctc
    47461 caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct tctttttaat
    47521 agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat tgaattgatc
    47581 ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat
    47641 acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata ctttcgcgaa
    47701 ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac ggatcattac
    47761 caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt gattgaaagg
    47821 cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac aatcgacaag
    47881 aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta tctatctgtt
    47941 ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc atctaagtac
    48001 tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact tgttacgcct
    48061 acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc tcaatacgaa
    48121 attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg
    48181 attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga ctagcagttt
    48241 caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga agagatatag
    48301 tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg tttttcgaaa
    48361 ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct tcggagcatt
    48421 cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca actgaaacag
    48481 ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag tcatcacaat
    48541 caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc gcaatcagct
    48601 ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta aaagaaaaaa
    48661 acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct gaaatcgtga
    48721 agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat cagaatctgg
    48781 ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact gagatcaaaa
    48841 aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc acgaatgtag
    48901 gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa gattggaaga
    48961 caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat ggggcaatta
    49021 ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg atcgattatc
    49081 tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat ccaacggctg
    49141 tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga caagtatctt
    49201 taataaataa cacattttaa taagtagttg aacacataac taccttactt gcaaaggctt
    49261 aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg cggacagttt
    49321 tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag tagaacgcgt
    49381 cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc cactacacta
    49441 aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag gggattggac
    49501 aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca gaaactagcc
    49561 atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga ggagctgttg
    49621 gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga gccggaatgg
    49681 cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac aaattaccta
    49741 aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca agtccatttg
    49801 aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata catcgatttg
    49861 aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc tacgcctatc
    49921 caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt aggtgcacgt
    49981 agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc ggaattagct
    50041 tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa agaaatcgca
    50101 caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt acaaacgttg
    50161 agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat tcctgatgtc
    50221 cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa aaatcagcca
    50281 aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga taaagaaact
    50341 gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac gatcctacaa
    50401 cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa agctttcttt
    50461 atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac tgagatagat
    50521 gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac agacttcggt
    50581 gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac agaacagaca
    50641 gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga tcaagaagac
    50701 aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga tgagatagcg
    50761 atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag ttcatcaaaa
    50821 agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca ctttttctag
    50881 tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag gttgagattt
    50941 ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata tgggtcgttt
    51001 taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg atgaaataaa
    51061 aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct ataaaatttt
    51121 acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa gtatggggat
    51181 cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt tgatcgacac
    51241 ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa gccttgaaaa
    51301 cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag aatattttgg
    51361 taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag tggctgatgc
    51421 attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg agaatatcat
    51481 cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta atctggaaaa
    51541 taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag cgataactgt
    51601 acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc accgtgcact
    51661 ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg agccatctat
    51721 ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta ttgggcgtgc
    51781 attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc tgaaacaagc
    51841 aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag tcgtttataa
    51901 ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca tcatggcaat
    51961 cgcgggcgga aaatcaaaag ccaaagcaat tcgagcttat atgaaaaatg caccaaaaca
    52021 aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg taacccttta
    52081 aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt aaagtaggta
    52141 ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat gtagatggaa
    52201 tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac ttgttaaaat
    52261 acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt tcattcaacg
    52321 taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta ccatggggcg
    52381 aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa tcagcagctg
    52441 aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc ggaaacgatg
    52501 ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa acagttatct
    52561 caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg aacgacaaat
    52621 ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac caaatgactc
    52681 tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct gcaaacatcg
    52741 ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa ttgaacggta
    52801 aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca gaattagtaa
    52861 cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa gaagcttcaa
    52921 acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt ggtatgactt
    52981 acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac aaacaattag
    53041 ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta gtacgtactt
    53101 tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta tagacatgta
    53161 catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata agtttattca
    53221 ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga caaaaaagtt
    53281 cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga cgacactcgt
    53341 atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa agcaatcctt
    53401 ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc tttagctcca
    53461 gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc tgaaactcgc
    53521 ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt cgtattcgaa
    53581 aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac agaactaggt
    53641 aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac agctcaccgt
    53701 gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc tggattcttg
    53761 atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg accaatgatt
    53821 gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa cttgattcct
    53881 aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga agcaaaagga
    53941 atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa agaattaatc
    54001 gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc agaattttca
    54061 aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat ggctttagac
    54121 atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa aacagttgtt
    54181 tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac aatcggtgtt
    54241 tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg agactcagcc
    54301 gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac aggtggcggc
    54361 gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat caacgataaa
    54421 taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa gggagttttc
    54481 atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc gtaaacctat
    54541 tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat ttgctgaagc
    54601 agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat ctcctgcatt
    54661 attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct cagcacaaaa
    54721 ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag ctcttgctga
    54781 tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt tccatgaaac
    54841 agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga caccaatcct
    54901 atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat ggatcgaagg
    54961 acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca tggttattgc
    55021 ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa tcgctgacga
    55081 aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag tatcagaagc
    55141 agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat acatggcaaa
    55201 agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt cattcttgtc
    55261 attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga aatgtttgca
    55321 ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa aaaacaaact
    55381 caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag atcttagact
    55441 cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct tttggccgcg
    55501 gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta cgtgacggag
    55561 acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta aacaacatca
    55621 ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac aaagctatga
    55681 tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt ttaggtgttt
    55741 ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac cactacctag
    55801 gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac ggcggatcac
    55861 atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc gctccaacat
    55921 tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca atcttgaaag
    55981 ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac cttggttcaa
    56041 acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat gtacctggta
    56101 aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa gaaaaaggtg
    56161 tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg atcaaattct
    56221 acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta gacgaaaacg
    56281 actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa ttagttggtg
    56341 acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa ggaattgcta
    56401 actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc gaagctatcg
    56461 aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt gaaacagaag
    56521 attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa actggttctc
    56581 tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa gaccaacttg
    56641 gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac aaataattct
    56701 tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc acttagttaa
    56761 gtaatcgaac tctctacttg ctgaaatatc agcaggtaga gagttttttt tgcaactagc
    56821 ttctgtatat gatgtttaca aagaatggta caagaaaaga tcatccttga aaaaggaaag
    56881 ctaacaataa ctagcatcat ataagagctt ttaaggatag aacagtagaa taggaatcta
    56941 tgagcaagtc ctctcgtata aataaaatag gagggaagta gcagcatatt tttcataaag
    57001 tggctctctt tcttcatttt tactttggtt gctcctattt gaaaattcga gcacactcaa
    57061 atacaataaa aagaaatggc aagggagagg attagatgaa aaaagaagaa aaaatcgcga
    57121 ttttacaaga gatcatccga attaaatcag tgaatggaaa cgaaggtgaa gtagcagcct
    57181 atttgaataa gcttttggca aagcatgaca taacaggaga gattgtttct tacagagatg
    57241 gtagagataa tttgattgct cgttaccaaa agggccaatc aggaaaagtg ttaggcctat
    57301 ctgggcatat ggatgtcgtg gcggctggtg atgaatcgtc ttggacctat gcaccctttg
    57361 cagcagaaat ccatgggaac cgtttgtacg gacgtggtgc tacagatatg aagagtggct
    57421 tggcagcaat ggtgattgcc atgatcgaac tgaaagagtc aggaaaacca tttaacggaa
    57481 cagtcaaatt attagccact gtcggagaag aagtgggtga actaggtggg gaacagctca
    57541 cgaaagcagg ctatgtagat gacttagatg ccttgatcat cggagagcca actaattaca
    57601 gtttgatgta tacgcacatg ggatcaatca actatacagt cacctcgcac ggaaaagaag
    57661 cgcatagctc tatgccagac caaggttata atgcaatcaa tcacctaaat gaattcatta
    57721 cgaaagcaaa tgctgaaatg aatcacctcg ctgaaacaat cgagaatcct gtcctaggaa
    57781 aaacaatcca taatgttaca ttgatcagtg gcgggaatca agtcaacagc atcccaagcc
    57841 atgcgcagct ccaagggaac attcgatcga ttccagaata cccaaatgat aaaatcattg
    57901 cattgctgca atcaatcgtc aatgaattga atcaagagac agactatcat ttggaattga
    57961 tgatcgatta caacaaaata cctgtcaaag ctgatccaga ttctcctttg attcattgca
    58021 ttcagcaaca attcagccag ccattacctt tagttggagc ggcagcaaca acggatgcag
    58081 ccgaatttac gaaagctaac cattccttcg actttgtcgt ctttgggcct ggtgtcgtta
    58141 ctttgcctca tcaagtagat gagtatgttg aaatcgataa ttatttggat atgattgaaa
    58201 aatatcaagg aattatttta tcctacttag cgtaagattt ttttaataaa tgaaaaaaca
    58261 gagccagatt agcattattt tgctaatcca gctctgttta ttcagcctat ttttcttttt
    58321 gaatatatca agcgttgtgg caacatagtg atgactaatg aaacaacgac gatagacaac
    58381 aaacgatcaa ggtagtctgt acctgcctgt accaagatca cacttgttgt ctgacttaaa
    58441 cctaacccat gtaatagctg aacgatcata ctggatcctg aggaggtgat cccgccaaat
    58501 aactgaacgg taatgatcga agaaacgatc gttccaggta ccgacaaaat caaagcgagt
    58561 aataacagtt tcaagctgtt tttatctttg ccgagatatc ttggcaaaat caatccagca
    58621 agcaagcctg tgagaatttg gattggcgaa taatacagag aaaaaatatc tgtagtcact
    58681 ccacttaaaa taccgctgga caatcctgtc agcattccag gtacaggccc aagcaatgca
    58741 ccagcaaaaa tcgttccgat agaatcgaga tagattggca aacgaagcca taaagcaatc
    58801 gttcctccaa tataattgat cccaatacac aaagccatca ctgtaagtac gcgagttgcc
    58861 attttgtttt tattcatttt gttagcccat ctttaacctt tccaaatctt tcataatcgt
    58921 ttcttcttgt gcatttaaaa gaacggttaa aaactttcgg aaaaaaagac gagtatctac
    58981 ttttgtcata attttactgt tagcaggttt ctgccaaaat tcataacgat caaccaatgt
    59041 ttgtccacga cttattcctt gagtttccac agcagtgaag cttttgaaac cttcacagat
    59101 ggccccctcg ataaaataag caacagctaa cggatcattg atgacacatc ctaaaattct
    59161 ttcttgtttc cagtggaagt caaaataaaa acgagtgatc gcttttagat actctccctc
    59221 ttcaggattc atttgacagc agtattcgag gatagtcggt gtcaagacga tttctcttgt
    59281 cacatctaac ccaaccattt ctatcgtttg tttgagattc tcgaatacat aagaagctgc
    59341 ttcaggatca caccagtaat tgtattcagc aactggcgaa cagttaccat ggcttttgta
    59401 agttccaccc atcgagacaa aacgatccat atgtttccca atatttgggt tgactttcaa
    59461 agcaaaagcg atattggtca aaggacccaa agcaatgact gatgtatttg tcttttcttt
    59521 gaaataatca gccaaaaaat caacggcatg gagaggttct gcctgttttc tcaagatcat
    59581 cgggaaattt gtttccccta aaccatccat accatgagtg tcttgtgcac tgatgaaagg
    59641 ttgcttcaaa ggtttttcca ttccttgata aacaggaatg tctaaacgat cacagcgttc
    59701 caggcatttc agcacattct gcgtacctat atctgcaggg acattcccac aaactacggt
    59761 aatagcaact acttcgatat caggggattt gacagctaat gataaagcta aagtgtcgtc
    59821 gatccctgga tcgcaatcaa taattacttt tcgcattatt tccaatctcc tttctttaga
    59881 gatacttagt tttttattct gggaagtttc cgaaccagtc caagacgagc gtcttgatta
    59941 taatgcacga aattagtata ctggctttta ttgcgaatgt aaatgctctc ttacaagtaa
    60001 atctattgag gagaacttta agtaaatgat acaatgaaaa agaaatggaa tttacgtgtg
    60061 ccgaaaataa agaatcctat ggatcggtac attggataat agttagatta tagtttggca
    60121 gaaagcggcg ggtgtacaat ggttattttt tatttcattc ttattctgat aattataggg
    60181 atcagttacc attttctttc ttatttttat gcaaagaaat tagtaagagc gggagtacag
    60241 agaggatatg actggtatga agacactggt catcgtttga tggaccctgc tgcagtcacg
    60301 tcagtagaaa aaaaacgaca aaaattagaa gaagagtgtg aaagcttttg ggagcaggga
    60361 acacccaaaa caatcaaaag ttatgacggg ttgaaactag ctggtcagat gttcatccat
    60421 ccaaataagc agaataaatg ggtcatttgc gtacatgatt accgaagcac agggaaaaga
    60481 gacatgtctc atataggtaa aagatatgca gagaaaggat tcaacgttct tgtacctgat
    60541 ttacgtgcgc acggagaaag tgaaggagaa atcatcggaa tgggatggct agatcggcta
    60601 gatttaattg cctggatcca actcatctta gatgaacagc cgaatgcgtc cattatttta
    60661 cacggcggat caatgggagc ttctacgatc atgatggcaa gtggtgaaaa acttccatca
    60721 gcggtcaaag gatttatttt agacagcgga tatgtttctg tttatgcaga attccgatat
    60781 atgttaagta aaatcactgt ctttcctaaa aaaatggtca tgagatatgc gaatcattat
    60841 gcccagaaat acgccggtta ctccttaaag caagcatcag ctacgagaca gcttggcagc
    60901 aaccatctgc ctttattggt cattcatggt gaaagagatc attttgtccc tacagaagca
    60961 gcgtacacga ttcaaaatgc tacggcaggg gataaagcac tgctgcttgt tccagaagca
    61021 gaacacttgg aagcatcgat gaaagatccg aaaacctatt ggacagtgat tttttcattt
    61081 attgaacaaa gaataacgat gaattaattt tctcttcttt tttgtaagta attcgaggat
    61141 aaataattgt ttatagatca ggagactgct aaaataaggt aggaataaaa aaatatcagt
    61201 tatttgagga gtgcggcata tggaaatcaa agaagaaaaa aatcgtcttg ctttattcaa
    61261 tgatgaacaa caagagatcg gagaaatgac atggtcagat gcgggcccag acatcatgat
    61321 cattgaccat acatttgtag atccagcata tcgtggtcaa aagttagctg aaaagctagt
    61381 gtatactgga gtagaattag caagacgaga cgggaaaaag atcattcctt tatgccctta
    61441 tgcaaaaaaa gagtttgaaa agaaaccaga atatcaagac gtcttacgtc agtcataaga
    61501 ataacagctg aaagttttga actttcagct gttattccat ttatatagag ttgtcacgga
    61561 cttttatcta tgataaagtt aaagagacga tatcttttgg gaaggaggct gcgatatgta
    61621 taatttaatt ttgggaatcg tgatcgtttt atcgattgtt atggtcattg cgattatgat
    61681 gcagccaagc aagcaaaata gtgcagcgag tgcatttacc ggcggtgcgg atcaactatt
    61741 cggcaaacaa aaagctcgcg gctttgaagc tgtcatgcag cgatcaactg cagttatggg
    61801 agctatctgg atgatcttat tatttgtcct agcatattta tcttcgaagt agttgaataa
    61861 gcctctcgca aaacggcggg cggctttttt tattgcaaag agaacgctta agaagaagtt
    61921 atgaaagaaa ctatggtaaa atgaggcgaa agaggtgaga cgagtgagac aattaccaaa
    61981 acccctttat gcaaaacatg gaaagcgagc ggttttgctg cttcacgctt actcaggaag
    62041 tccaaacgat gttcgaatgc tcgcacgatt tttggaaaaa gcagattata cagtttatgc
    62101 gccgcttttt aaaggacacg gaacaatgga gccatatgat attttgcaag aaaaagcaga
    62161 aagctggtgg gcagacacta aaaaagccat ccatttttta cagtctgagc agttttctga
    62221 tattgccgta ttaggattat caatgggcgg catctttgct gtacgagcac tagaagaaga
    62281 atcagtgatt ggcggcggtt ttttctgttc gcctttgtct cctgtaaaaa caaacgtgcc
    62341 tgaaaacttt gaaaaatacg tacgtcaagt gctgaagaca gcaggtaaat cagagaaaga
    62401 aatcaatgaa aaagcagttg cttaccgctc gttagctgaa caacaattaa tggatataca
    62461 agatcaggca gcaatcgtag aaagtaggtt gtcagatatc caacagccga tatttttagc
    62521 tcaagctggg aaagatgaaa tgatcgaccc taatggcgta tttgaaactg cacgcaagtt
    62581 gagccgtcag cgagtgacac ttcgatggta tcctgaaagt ggtcatgtga ttacagttgg
    62641 tacagcaaga cgcgaattag aaaaagacgt cttggaattt ttagaaaaat taccttggaa
    62701 tgaggaataa atatgacaaa agaaacgata aaagagaaaa ttctcttttt catggaaagc
    62761 agtcgcaaac gcagtttttc tatggaagaa atcgctgaag gtttagggtt ccaaaaaagc
    62821 gatgatttta aattactagt acaaacgatt gctcaaatgg aaagagaaca gagtatagtt
    62881 ttcaataaaa aaggaaaagt gaaacttcct ttaaaaccaa tcttgatcga aggaacattt
    62941 cgagcaaatg aacgaggatt tggatttgta acgattgatc ccgaagaaga tgacatctat
    63001 atcccaaaag aagcgactgg ttttgcaatg gatggagaca ccgtagcaat cgatatcatg
    63061 aagcgagcaa atacagatat ggatcgcgga gcagagggga aagtagtcga gattcgagac
    63121 cgggcgacta ctcagttagc aggggaattc gttgcttatt cagaagaaga aatacaagaa
    63181 acggatcttt acggcgttgt tatcccgaaa gatagaaagc tcaaccaatt taaagtatat
    63241 gcagcagcag agggagttcg tccagttgac ggaagtattg tgctgatcga actgacccat
    63301 taccctgaaa aagggtatgc gactagttta gaagggataa tcaagcaagt gattggtcat
    63361 aagaatgacc ctggcatgga tatcctctca attgtcgtgg ctaatggtat ccctacgaaa
    63421 tttcctgatg atgtattgtc tgaagcagat gaagttccag atcaaatcag cgaaaaggat
    63481 ataactggac gtagagattt aagaaatcag ctcattgtta cgattgatgg agaagatgca
    63541 aaagatctag atgatgcagt tacggttaaa aaattagata acggtaatta ttttcttgga
    63601 gtacacattg cagatgtttc ttactatgtg acacaaggca gtcaattgga cagagaagca
    63661 tatgaaagag ggacaagtgt atatttgact gatcgagtga ttccgatgat cccgcaacgt
    63721 ttatcgaatg gtatctgttc actgaatccc catgttccaa ggctgaccat gagttgcgaa
    63781 atggagattg atccaaatgg acaagtagtc tctcatgata ttttcccaag tgtgatccaa
    63841 acaacagaac ggatgaccta cacagctgtc aatcaaatct tagaagatca agacgaagaa
    63901 acgatgcaac gctatgctca gctcgtacct atgttccaag aaatgggtga attgcatcga
    63961 atccttgaaa cgatgcgaat caaacgtggc gcgatttctt ttgaggatcg cgaagcaaaa
    64021 atccttgttg atgctgaagg acacccaaat gatatccagc taagaagcag aggaattggt
    64081 gaacgcttga tcgaatcttt catgcttgcg gccaatgaaa cggtagctga acattataat
    64141 aaattaaatc tgccgtttat ttaccgaatc catgaacagc caaaagaaga aaaaatgcag
    64201 cgcttctttg attttgcttc agctttgggc attctggtcc gaggaacgaa aggaacgatc
    64261 acaccaaaag atttacagca tgtgatcgaa ggagtagaag acaaaccaga agctgcagtt
    64321 atcaatacta tgttgctaag aagcatgcaa caagcacgct attcagaaga caattttggg
    64381 cattatggct tagcggcaga ttactacaca cactttacat ctcctattcg acgctatccc
    64441 gatctaatcg tccatcgtct gatccgttct tacagtgaag ataaaagtga aagcaatcaa
    64501 agttcgtggg ctacggaact tcctgaaatt gcagatcata gttctaagat ggaacgtcgt
    64561 gcagtagaaa cagaacgtga agtagattcc atgaagaaag cagaattcat gatggacaaa
    64621 gtgggggaag aattcgatgg gatcatcagc tctgttgtga aattcggcct gtttatcgaa
    64681 ctgccgaata cagtagaggg attgatccat atcaatgaat tgaaacagga ctacttccat
    64741 tttgttgaaa atcatttagc tcttgtaggt gaaagaactg gtctgacttt taaaatcgga
    64801 cagaaagtgc gagtaaaagt agtcaaagct gatccagatg aacgagcagt agattttgaa
    64861 cttgtttccg cagaagaggt tacagcgttg gaacggccaa aaacacgaaa tggaaaatca
    64921 ggaaaacgcg aaggaaaaca agcaggaaaa cgcccagaaa gaaatggcaa gaagaacaaa
    64981 cgatatagcg atagtaagca aacgttcggt aagaaaaaag ggaagaagcc attttacaaa
    65041 ggtgttaaaa agaagaaaaa gaaaaaataa aatgagttgg aggaaacagc tatgccaaaa
    65101 ggcgagggaa aattaattgc acaaaacaag aaagctcgcc atgattattc gatcatcgac
    65161 acgatggaag cagggatggt cttgcaagga accgagatca agtcgatacg aaacagccgg
    65221 atcaatctaa aagatggatt tattcgcgtc cgcaacggag aagctttctt gcataatgtt
    65281 catatcagtc cttatgaaca aggaaatatt tttaatcatg atccgttgcg cacgagaaag
    65341 ttattattac acaaaaaaca aatcatccgg cttgaaaatg aattgaaaaa tactggaatc
    65401 actgttgttc ctttaaaagt ctatattcgt aacggctatg ccaaggtatt gattggtctg
    65461 gcgaaaggga aaaaatctta tgataaacgg gaagatttga aacgaaaaga tatcgatcga
    65521 caaattgatc gaacattaaa aaatttctct agataatttc attatccttt ttcgcatatt
    65581 cggtgctttt gcttttactt aatgataaaa tttgttagca tttaatctga taaaacatga
    65641 ttatcatagg ttttttctta gatcaggagc accgtgtata aagaaaaatt cgcttcgttt
    65701 ttgactgaaa taaaaaagtg tctaattttc atgaaaatgt tttgaatttt ctgtttcatg
    65761 gtggtatgat accgtgtggt tagaaaccga ttggaaaaaa ccagatacgc acatgaattg
    65821 attttatgtg aatttcgggg aaaagaggtg aaatggattg gatgaacgct cgctaacgtt
    65881 ccacattgga ccaatttggt ttgatggtac agtttgtata atggtgttac tgacctgttt
    65941 gattgttttc ttcttagctt acttttttac aagaaatctt aagttgaaac caacaggcaa
    66001 acaaaatgcg atagaatggg tcattgattt tagtcgagga atcgtaacag ataatctgcc
    66061 tagaaaagaa ttgagcaatt tccatttgtt ggcatttaca ttgtttttat ttgtttttgt
    66121 tgcaaacaac atcggtttag taacaaaaat cgttttacct aacgaaacaa cactttggaa
    66181 aagcccgaca gcagacccgt ttgttacttt aactttggca tttataatga tcttacttac
    66241 tcacttattt ggggtgaaaa agttaggctt caaaggatat ttcgtaaatt cgttcttgaa
    66301 accatatagt tttatgtttc cgatgaaact gattgaagaa tttacaaact tattaacact
    66361 tgctttacgt ctttatggga atatttatgc gggggaagtt ttgctaacct tgattgcaaa
    66421 aatggtaata agcttaggct ggttctcatt gccgttagca attccattag agatggtctg
    66481 gatcgcattc tccttgttta tcggaagcat ccaagcattt gtcttcgtta ctttatctat
    66541 ggtttatatg agccataaga ttgaagtgga agaataatat tcgttttaaa aacaaactac
    66601 tatttatcta atttaggagg aaaacaatta tgaattatat cgcagcagca atcgcaatca
    66661 tgggagcagc aatcggagcc ggttatggta acggtcaagt tatctctaaa acaatcgaat
    66721 caatggctcg ccaacctgaa atgtctggtc aattccgtac aacaatgttt atcggggtag
    66781 ctctagtcga agctgttcct atcttagggg tagttgtcgc cttgattcta gtttttggtg
    66841 tttaattgaa tcagtattaa ggcagaaaca atttggtttg ttactgcttt tttacatgaa
    66901 aactagaagt acttcaaaga aaggaaggct cgcctatgct gaatcaattg gcaattgcag
    66961 aagttggcaa tgcgatgtta ggaaatgtca tcgttgtcag cggctcattt ttgatactac
    67021 tggctctctt gaagcatttt gcttggggac caatcagcga tattttgaaa aaacgtgaag
    67081 acaagatcgc caatgatttg gattctgcag aacaatctcg catcaactca gcgaaaatgg
    67141 aacaagaacg cgaacaacaa ttgttagcct ctcgttctga tgcagctgat atcatcaaaa
    67201 atgcgaaaga aagtggagaa ttaagccgcc aaaatatttt gaaggatgct caagaagaag
    67261 cagctcgtct aaaaagtaaa gcccaagctg atatcactgt agaacgtgat tcagcgctga
    67321 actctgtaaa agacgatgtt gcagaactat ctcttcaaat cgcggaaaaa atcttgaaca
    67381 aagaattatc tccagaaatg cacgaatcat taatcaatca atatatcgaa ggtctaggtt
    67441 cttcaaatga aactagataa atatacagta ggaagacgtt acgggaaagc tttatttgaa
    67501 ctggcgatcg attcaaataa agcagaagaa atttatcaag atctcgtaag cttacgtcaa
    67561 atctatgaac aagtacctgg attaggtaat atgttaagtg atgttcgtct agaacctcat
    67621 gaaaaacgag aaatcatgga caagcttgtt agtggctatg atggaatcgt taaaaacttt
    67681 ttagaagttg tttatgcgta taaccgaatg gatgatcttt tgttcatgat cgaagaatat
    67741 gaacgtcgat atgatgaaca tcaaggtttg ctgttgggaa gtgtcacaac agcagttcca
    67801 ctttcagacg aacaacgaat acaattagaa aagaatgttg caaaaacaat gaactatcaa
    67861 tcagtcgaat taaaacaaat cgttgattct tctattattg gtggcgcgat tgttgaagct
    67921 aaccatcgag taatcgacgg aagcattcgc acgcaattag aaaatatgcg taatcaatta
    67981 aatagatagt acaaagaggt aggtgaatcg aatggccatc aaagcagaag aaattagtgc
    68041 cttgattaaa gaacaaattg aaaattatca aaacgtgctt tcagtcgaag aaatcggtac
    68101 cgtaacttat gtaggtgacg ggatcgctcg tgcacatggt ttagaaaacg cgatgagtgg
    68161 tgagttgctt gaattttcaa atggctcata cggaatggcg caaaacttag aatcaaatga
    68221 tgtaggtatc attatccttg gcgatttcga gtccattcgt gaaggagata aagtaaaacg
    68281 aacaggaaag atcatggaag ttccagttgg agaggccttg attggacggg tagttaatcc
    68341 gctaggtcag ccaattgacg gactaggtga aatcgttact gacaaagctc gtccagtaga
    68401 agcaatggca ccaggcgtta tgcaacgtaa atctgttaac gaaccaatgc aaacaggtct
    68461 aaaagcaatc gacgctcttg taccaatcgg acgcggccaa cgtgaattag ttatcggtga
    68521 ccgtaaaaca gggaaaactt ccattgcgat cgatacgatc atcaaccaaa aaggtcaaga
    68581 tatgatctgt atctatgtag caatcggaca aaaagattct acagttcgta cacaagttga
    68641 aacattgaaa aaatatggcg caatggatta cacaatcgtt gtgaatgccg gtgcgtctca
    68701 accagcgccg ttgctttata tcgcaccata tgctggtact gcaatgggtg aagagttcat
    68761 gtacaacggg aaacatgtct tgatcatttt tgatgatctt tcaaaacaag ctgttgctta
    68821 tcgtgaactt tccttgctgc ttcgccgtcc accaggtcgt gaagcttatc caggggatgt
    68881 tttctactta cactcacgct tattggaacg tgcagcaaaa ttaagtgatg atttaggtgg
    68941 cggttcaatg actgccttac catttgttga aacacaagct ggagatatct ctgcttatat
    69001 cccaacaaac gttatttcga tcactgatgg acaaatcttc ttagaaagcg acttgttcta
    69061 cgcaggtact cgtccagcgg ttgatgccgg actatctgta tctcgtgtag ggggttctgc
    69121 gcaaatcaaa gcaatgaaaa aagttgctgg gactttacgt ctagacttag caagttaccg
    69181 tgaattagaa gcatttacac aattcggttc tgacttggat gcagcaacac aagcaaaact
    69241 aaatcgtgga cgccgtacag tagaaatctt gaaacaaaaa ttgcatgcac cgctagctgt
    69301 tgaaaaacaa gtagtgattt tgtatgcttt gacacatgga ttcttagata gtatccctgt
    69361 agacagtatt ttagacttcg aacatgaatt attcgaatat ctggatacca atcattcaga
    69421 tatctttgaa acaatccgta caacaaaaga tcttccagaa gaagaagcgt tgaacagtgc
    69481 gattcaggaa tataaagaca tgttcttagc tacaaaagga aataattcct ctacagaaga
    69541 taaattgaaa tccattcaaa atgcatagtg aggtgagatg aatgggtgct tcattaaatg
    69601 aaatcaagac acgtatcgct tcaacgaaaa aaacgagcca aatcacgcgg gcaatgcaga
    69661 tggtgtctgc ttctaagttg acaaaatctg aagcttcttc acagaagttt caggtttatg
    69721 caacaaaagt aagagaaatc gttacgcatt tgactgctac tcagttaaat gatattgcct
    69781 cagaaaatcc tagaggggat atcaattaca acagcatgtt gctcagccgt cctgttaaga
    69841 aaacgggtta tatcgtcatc actgcagatg gcggtttagt cggtggatat aacagctcga
    69901 tattgaaaca aacgatgtct attttagaag aagaccacca atcacccgat gattacgtga
    69961 tgatcgcaat tggcggaact ggtgcagact tctttaaagc acgcggtatc aatctagcat
    70021 atgagctgcg caatctatct gatcagccaa gctttgatga agtccgcaaa atcgtaaata
    70081 tggcaacaac tatgtaccaa aacgaagtat tcgatgaatt atacgtatgt tataaccatc
    70141 atatcaattc gctaactagt cagtttcgtg tagaaaaaat gctgccaatc tcagacttag
    70201 atccagaaga agcaactact ttcgaacaag aatatatttt tgaaccttca aaagaagaaa
    70261 tcttgtctca gttattgcct cagtatgctg aaagtttgat ctacggtgcg atcgttgacg
    70321 ccaaaacagc agaacatgct gctggtatga cagcaatgaa aacagcaact gataatgctg
    70381 ctaatatcat tgatgagctg acggtttctt acaaccgtgc aagacaaggg gctattaccc
    70441 aagaaattac cgaaattgtt gccggagctt cggcactaga atagtattga gattggagga
    70501 aaaaagatga gttcaggcaa gattgttcaa gtaatcggtc ccgttgtcga cgtggaattt
    70561 tctttagacc aatccttacc agatatcaac aacgcattag ttgtttataa aaatgacgaa
    70621 aataaatcaa aagttgttct tgaagcagcg ttagaattag gggacggagt gatccggacc
    70681 attgcgatgg aatcaacaga tggtttacaa agaggaatgg aagtcattga tacaggcaaa
    70741 gcaatctctg ttcctgtagg taaagaaaca ttaggtcgcg tattcaacgt actaggagat
    70801 acgatcgatt tagaaacacc tttcccagaa gatgcggaaa gaagcgaaat tcataaaaaa
    70861 gcaccagcct ttgacgaatt aagtacaagt acagaaattt tggaaacagg gatcaaagtt
    70921 atcgatttgc ttgccccata tttaaaaggt gggaaagtcg gactattcgg tggtgccggt
    70981 gttggtaaaa ccgtactgat ccaagaactg atccataata tcgcccaaga acatggtggt
    71041 atttctgtat ttaccggtgt aggtgaacgt actcgtgaag gtaatgactt gtattatgaa
    71101 atgaaagatt caggagttat cgaaaaaaca gccatggtgt tcggacaaat gaacgaacca
    71161 ccaggtgcac gtatgcgtgt tgctttgact ggtttgacga ttgcggaata tttccgtgat
    71221 gtagaaggtc aagatgtact gttgtttatc gacaacattt tccgtttcac tcaagctgga
    71281 tctgaagtat cagccttgtt aggacggatg ccttctgcgg ttggttatca accaacattg
    71341 gcaacagaaa tgggtcaatt gcaagaacgt atcacatcta cgaaaaaagg ttcgatcaca
    71401 tcgatccaag caatctatgt gccagccgat gactacactg acccggcgcc ggcaacagca
    71461 ttcgcccatt tggatgcgac aaccaacttg gaacgtaaat taacagaaca agggatttat
    71521 ccagcggtag atccattggc ttcttcttca agtgctttag cacctgaaat cgtgggagaa
    71581 gaacattata aagtagcaac agaagtccaa cacgtattgc aacgatatcg tgaattacaa
    71641 gatatcattg ctatcttagg tatggatgaa ttatccgatc aagaaaaagt gttagtttct
    71701 cgtgcccgca gagtccaatt cttcttgtca caaaacttta acgtggctga acaatttact
    71761 ggtcttccag gttcatatgt ccctgtagaa gaaacagtga aaggattccg cgaaatcttg
    71821 gaaggcaaat acgatgatct tcctgaagaa gctttccgaa gtgttggccg aatcgaagac
    71881 gtcgtagaaa aagcgaaaac actaggctac tagaaagggg tgccccatgg atcaatattt
    71941 aacagtaaat gtggtaactc ctgccggttt ggtctatgat caccatgcag ctatcgtcgt
    72001 tgcacgtacg acagctggtg agcttgggat tttgcctaaa catgcaccaa tcatcgttcc
    72061 attgacgatt gatgaagttc gtgtaaaacg aacagattca gatacccacg tcgattggat
    72121 cgctgttaat ggcggaatca tggaagttcg tgataatgtt gtttcaatcg ttgctgatag
    72181 tgcggaacgt gaacgtgata tcgatgtatc tcgtgcggaa cgtgcaaaac aacgcgcaga
    72241 acgccaaatc gaagaagcaa aagcaaaaca agatacgaac gaattgaaac gggcaactgt
    72301 tgcgttgcac cgagcaatca atcgtatcaa agtatctaag cactcataaa atgagatgcg
    72361 gatgtttatc cgtgtttaag aacagtaaaa agaggttatg acagaagtgg tcagcttcaa
    72421 gacataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaatct cagcttattt
    72481 ttcgaagaag ctacttctgg aacaccgtat atcggaggtt gtgaaatcag cgttttgtcc
    72541 tgagtattag agaaacaaat ggaaaatagt tcctcatatt ttgccgtttt ttgagctaat
    72601 accgagggtc agatgattga acaccgttta tttggaataa gggactgcga caagactttt
//
LOCUS       DACEDO010000006        66842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEDO010000006 DACEDO010000000
VERSION     DACEDO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.006"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..428
                     /locus_tag="IWO52_000428"
     CDS             <1..428
                     /locus_tag="IWO52_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0354721.1"
                     /translation="IQPETVLPRFAKRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKG
                     KFVALITLRNIGFWLAKESQKGPVDLTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEA
                     MFREQGTLEAVLITKDGNPDGNLLGIVRPRDIYKQIEKD"
     gene            433..897
                     /locus_tag="IWO52_000429"
     CDS             433..897
                     /locus_tag="IWO52_000429"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0354722.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            894..1805
                     /locus_tag="IWO52_000430"
     CDS             894..1805
                     /locus_tag="IWO52_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0354723.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            2040..2579
                     /gene="pyrR"
                     /locus_tag="IWO52_000431"
     CDS             2040..2579
                     /gene="pyrR"
                     /locus_tag="IWO52_000431"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0354724.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            2593..3876
                     /locus_tag="IWO52_000432"
     CDS             2593..3876
                     /locus_tag="IWO52_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0354725.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            3889..4815
                     /locus_tag="IWO52_000433"
     CDS             3889..4815
                     /locus_tag="IWO52_000433"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0354726.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            4834..6114
                     /locus_tag="IWO52_000434"
     CDS             4834..6114
                     /locus_tag="IWO52_000434"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0354727.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            6119..7195
                     /gene="carA"
                     /locus_tag="IWO52_000435"
     CDS             6119..7195
                     /gene="carA"
                     /locus_tag="IWO52_000435"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0354728.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            7200..10385
                     /gene="carB"
                     /locus_tag="IWO52_000436"
     CDS             7200..10385
                     /gene="carB"
                     /locus_tag="IWO52_000436"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0354729.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            10439..11215
                     /locus_tag="IWO52_000437"
     CDS             10439..11215
                     /locus_tag="IWO52_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0354730.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            11212..12144
                     /locus_tag="IWO52_000438"
     CDS             11212..12144
                     /locus_tag="IWO52_000438"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0354731.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            12141..12857
                     /gene="pyrF"
                     /locus_tag="IWO52_000439"
     CDS             12141..12857
                     /gene="pyrF"
                     /locus_tag="IWO52_000439"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0354732.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            12864..13493
                     /locus_tag="IWO52_000440"
     CDS             12864..13493
                     /locus_tag="IWO52_000440"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0354733.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            13481..13795
                     /locus_tag="IWO52_000441"
     CDS             13481..13795
                     /locus_tag="IWO52_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354734.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            13833..14474
                     /locus_tag="IWO52_000442"
     CDS             13833..14474
                     /locus_tag="IWO52_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0354735.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            14505..15209
                     /locus_tag="IWO52_000443"
     CDS             14505..15209
                     /locus_tag="IWO52_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0354736.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            15206..16108
                     /locus_tag="IWO52_000444"
     CDS             15206..16108
                     /locus_tag="IWO52_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0354737.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(16266..17972)
                     /locus_tag="IWO52_000445"
     CDS             complement(16266..17972)
                     /locus_tag="IWO52_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0354738.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            18162..18785
                     /locus_tag="IWO52_000446"
     CDS             18162..18785
                     /locus_tag="IWO52_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354739.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            19235..20284
                     /locus_tag="IWO52_000447"
     CDS             19235..20284
                     /locus_tag="IWO52_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0354740.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            20281..21162
                     /locus_tag="IWO52_000448"
     CDS             20281..21162
                     /locus_tag="IWO52_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0354741.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            21165..21935
                     /locus_tag="IWO52_000449"
     CDS             21165..21935
                     /locus_tag="IWO52_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0354742.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            22214..23068
                     /gene="aroE"
                     /locus_tag="IWO52_000450"
     CDS             22214..23068
                     /gene="aroE"
                     /locus_tag="IWO52_000450"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0354743.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            23246..24409
                     /locus_tag="IWO52_000451"
     CDS             23246..24409
                     /locus_tag="IWO52_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0354744.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            24526..24936
                     /locus_tag="IWO52_000452"
     CDS             24526..24936
                     /locus_tag="IWO52_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0354745.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            25110..27338
                     /gene="topB"
                     /locus_tag="IWO52_000453"
     CDS             25110..27338
                     /gene="topB"
                     /locus_tag="IWO52_000453"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0354746.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            27476..27793
                     /locus_tag="IWO52_000454"
     CDS             27476..27793
                     /locus_tag="IWO52_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354747.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            27953..28189
                     /locus_tag="IWO52_000455"
     CDS             27953..28189
                     /locus_tag="IWO52_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0354748.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            28186..28585
                     /locus_tag="IWO52_000456"
                     /pseudo
     CDS             28186..28585
                     /locus_tag="IWO52_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(29252..30196)
                     /locus_tag="IWO52_000457"
     CDS             complement(29252..30196)
                     /locus_tag="IWO52_000457"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0354749.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(30492..31436)
                     /locus_tag="IWO52_000458"
     CDS             complement(30492..31436)
                     /locus_tag="IWO52_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0354750.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            31657..32871
                     /locus_tag="IWO52_000459"
     CDS             31657..32871
                     /locus_tag="IWO52_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0354751.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            32893..34908
                     /locus_tag="IWO52_000460"
     CDS             32893..34908
                     /locus_tag="IWO52_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0354752.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(34991..36364)
                     /locus_tag="IWO52_000461"
     CDS             complement(34991..36364)
                     /locus_tag="IWO52_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0354753.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(36390..36674)
                     /locus_tag="IWO52_000462"
     CDS             complement(36390..36674)
                     /locus_tag="IWO52_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0354754.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(36676..38691)
                     /locus_tag="IWO52_000463"
     CDS             complement(36676..38691)
                     /locus_tag="IWO52_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0354755.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(38870..39175)
                     /locus_tag="IWO52_000464"
     CDS             complement(38870..39175)
                     /locus_tag="IWO52_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0354756.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(39264..40295)
                     /locus_tag="IWO52_000465"
     CDS             complement(39264..40295)
                     /locus_tag="IWO52_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0354757.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            40502..41377
                     /locus_tag="IWO52_000466"
     CDS             40502..41377
                     /locus_tag="IWO52_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0354758.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(41529..42371)
                     /locus_tag="IWO52_000467"
     CDS             complement(41529..42371)
                     /locus_tag="IWO52_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0354759.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(42463..44340)
                     /locus_tag="IWO52_000468"
     CDS             complement(42463..44340)
                     /locus_tag="IWO52_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0354760.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(44337..45758)
                     /locus_tag="IWO52_000469"
     CDS             complement(44337..45758)
                     /locus_tag="IWO52_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0354761.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(45761..49150)
                     /locus_tag="IWO52_000470"
     CDS             complement(45761..49150)
                     /locus_tag="IWO52_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0354762.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            49407..50798
                     /locus_tag="IWO52_000471"
     CDS             49407..50798
                     /locus_tag="IWO52_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0354763.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(50871..51518)
                     /locus_tag="IWO52_000472"
     CDS             complement(50871..51518)
                     /locus_tag="IWO52_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0354764.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(51526..52212)
                     /locus_tag="IWO52_000473"
     CDS             complement(51526..52212)
                     /locus_tag="IWO52_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354765.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(52218..53291)
                     /locus_tag="IWO52_000474"
     CDS             complement(52218..53291)
                     /locus_tag="IWO52_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0354766.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            53513..54187
                     /locus_tag="IWO52_000475"
     CDS             53513..54187
                     /locus_tag="IWO52_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0354767.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            54192..55532
                     /locus_tag="IWO52_000476"
     CDS             54192..55532
                     /locus_tag="IWO52_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0354768.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(55681..56667)
                     /locus_tag="IWO52_000477"
     CDS             complement(55681..56667)
                     /locus_tag="IWO52_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0354769.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            57391..58422
                     /locus_tag="IWO52_000478"
     CDS             57391..58422
                     /locus_tag="IWO52_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0354770.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            58531..60063
                     /locus_tag="IWO52_000479"
     CDS             58531..60063
                     /locus_tag="IWO52_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0354771.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            60202..61014
                     /locus_tag="IWO52_000480"
     CDS             60202..61014
                     /locus_tag="IWO52_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0354772.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            61004..61489
                     /locus_tag="IWO52_000481"
     CDS             61004..61489
                     /locus_tag="IWO52_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0354773.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            61512..62057
                     /locus_tag="IWO52_000482"
     CDS             61512..62057
                     /locus_tag="IWO52_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0354774.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(62130..63773)
                     /locus_tag="IWO52_000483"
     CDS             complement(62130..63773)
                     /locus_tag="IWO52_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0354775.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(63902..66130)
                     /locus_tag="IWO52_000484"
     CDS             complement(63902..66130)
                     /locus_tag="IWO52_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0354776.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(66163..66642)
                     /locus_tag="IWO52_000485"
     CDS             complement(66163..66642)
                     /locus_tag="IWO52_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354777.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        20593 a        12198 c        13271 g        20780 t
ORIGIN      
        1 tgattcagcc tgaaaccgtg ctgcctcgat ttgcaaaacg tgtaacaggt tttgaacaag
       61 atcttccgtt acttgaactg ttaaagatag tagcagaaaa aaggtattca caatttccgt
      121 tatacaataa aggaaaattt gtagcattga ttactttacg taatatcggg ttttggctag
      181 caaaagaaag ccaaaaaggt ccggttgatt taacaaataa acgagcgttg gatttgatca
      241 tccaaaacgg aaagtacaca aattatcatt ttgttccagc taaaacacat atttacgagg
      301 ttgaagcgat gtttcgtgag caagggactt tggaagccgt acttatcact aaagatggga
      361 atcccgatgg taatctgctc gggatcgtac gacctcgtga tatttataaa caaatagaaa
      421 aggattgatt ttttgttaat tatttattgg atattcagcg ctttgctagt aggagtggat
      481 cagttggtca aatggtggat cactgataat ctagcgcttg gagagacgaa aaatcttata
      541 ccaggtattt tgtcactgaa tcatattcga aacactggag ccgcatggag tatgctagaa
      601 gggaagatgt ggttctttac tatagtgaca ctcattgcag tcgttgtgat tctcacattg
      661 atgattaaaa atcgtgaaaa aggtaatcgc tggttcatga ttggattgag tttgattcta
      721 gctggtgcga ttggcaattt tatcgaccgt attcgtcttg gctatgtagt agatatgttc
      781 cagacagatt ttatgagctt tccgattttt aacgtagcag atgtgacact tgtatgtgga
      841 gtgatttgta tgttaattta tattatttta gatgaaaagg atcaaagaaa aaaatgacag
      901 aacttaaaac gacgattcac catgaatctg gtaggatcga taaagtattg actaatttat
      961 tcaccgatta cagtcgttcg caaattcagc tgtggctgaa aaatggagcg gtttctgtca
     1021 atggacagcc agtcaaagca aattacaaag taaaacaagg agacgagatt actgtcttga
     1081 tccccgaacc agaaacatta gatgtggtcc cagaaaatat cccgttgacg atcgtttacg
     1141 aagatgaaga tgtagcggtg ataaacaaac ctcaaggaat ggtcgttcat ccttcagcag
     1201 gtcatacgca tggcacaatg gtcaatgcgc tgatgtacca tatcaaggat ctttctacga
     1261 tcaacggcgt tattcgtcca ggtattgttc accgaatcga caaagatact tccggactgc
     1321 taatggttgc caaaaatgac caggcacacg aatcattagc tcagcaatta aaagataaga
     1381 cctctttgcg caaatatgtt gctttagtcc atggagagat tcctcacgaa aaaggaacca
     1441 tcaatgcgcc aatcggccgt tcgaaggaaa accggaaaat gcaggcgata agagaagatg
     1501 ggaagtcggc tgtcacacat tttactgtat tggaacgttt cgaaggattt actctggttg
     1561 aattaaaact agaaaccgga agaacacacc agatccgtgt gcatatgaag tatatcggtt
     1621 atccgttagc tggagatcca gtatacggac cgaagaaaac tttgaaaggc aatggtcagt
     1681 ttctccatgc gcaaatgtta ggttttacgc acccgactac aaacgaaaag atgatctttg
     1741 aagcacctct tccagaagtt tttgaaaaaa ctttagaaaa attaagaaaa aacattgcat
     1801 tttaatttag ataacggtat agtaattaac aacgaaagca taaaacttaa tagaattcct
     1861 ttaagtttag tcccgtgagg ctaagaaggg tgggagcaag gatacgtatg gtgcgccata
     1921 agtagattgc tcattgacat agtatagcta tcagtgtatc agtatacgct cagtctaaac
     1981 attcctcctg tcctcttagg gcaggagttt ttttatgcaa aaaagtaggg ggaatgaaaa
     2041 tgcaagcaaa agaagtagta gatcaggtaa ccatgaagcg agcattgact cgaattactt
     2101 atgagattat cgagagaaac catagtatcc aagatatcgt attggtaggt atcaaaacac
     2161 gaggaatcta cattgcatca agaattgccg aacgcctgaa gcagctagaa gatatcgata
     2221 ttcctgtagg agaactggat atcactttat accgagatga caaaaaagaa aatccagaag
     2281 aaccggaact tcattcttct gacatcccag tttccttaga aggaaaagaa gttattttga
     2341 ttgatgatgt tttgtataca ggacgaacga ttcgagcagc aatggacgca gtaatggatt
     2401 ttggcagacc aagaaaaatt tcattagcag tcctggtcga tcgtgggcat cgagaattgc
     2461 caatccgtgc agattatgtt gggaagaaca ttccaacatc tcgggcggaa gagattttag
     2521 tagagatgca agagcttgat ggacaagatc gcatcatgat tttgaaagag gaagattaga
     2581 ggagagagaa acatgtcgga gaaaaaagga tttcataatg atgacgtggt gttagatatc
     2641 cacgatcgcc caaccccagg acattgggta ggattaagtt tacaacactt gttcacgatg
     2701 ttcggagcaa ccgtgttagt ccccatctta gtaggtatcg atccaggtat tgcgctagtc
     2761 agttcaggac taggtacatt ggtgtactta tttactacta aaggaaagat acctgcgtac
     2821 ttaggaagca gcttcgcttt cattgcagcg atgcagcttt tgatgaaaac agatggttat
     2881 ccagcgattg cacaaggagc agtcacaact ggattagtct atttgattgt tgcgctgatc
     2941 atcaaaaaga tcggttcagc atggctggat aaaatcttgc ctccaatcgt cgtcggtcca
     3001 gtcatcatgg ttatcgggtt gggacttgcg gcaaatgcag caacaaatgc tatgtataat
     3061 cagggcacgt atgatttcaa gtatattgct gtagcgctga tcacattagg attaacaatt
     3121 ttcttcaaca tgtggctaag aggttttctc ggattgattc cgattttact aggtattatt
     3181 agcggataca tcgtagcctt gctgtttgga atcgtggata ctaagccaat cgtagacgct
     3241 gcctggtttg ctgttcctaa ctttgagatt ccctttgtac aatatgaacc aaagctatat
     3301 cttggagcaa tcacaacaat ggcacccatc gcttttgtga cgatgacaga gcatatcggc
     3361 catcttatgg tattgaataa actaactaaa cgtaatttct ttgaagaacc gggtcttcat
     3421 aagacgttga caggtgatgg attagcacaa atcgtggcag cctttgtcgg aggtcctcct
     3481 gtaacaagtt acggtgaaaa cattggtgtt ctggctatca cacgtgtcca tagcgtgttt
     3541 gtgatcggtg gagctgcagt atttgcagtc attttaggat tcgtaggaaa attaagcgct
     3601 ttgattttaa gtatccctgg accagtcatc tcaggaatca gttttatctt atttggagta
     3661 attgccgcaa gtggaatgaa gattttaatc gagaacaaaa tcgattttga taaaaagaaa
     3721 aatctgttga ttgcttcagt tatcttagta gtaggaattg gcggactcgt ctttgaagta
     3781 ggcacattta cattgtcggc aatggcatta gcaactgtac ttggcatcat tttaaatctt
     3841 atcttaccag aaacttcacg aagcgaagaa aactaaggag gaaacattat gatcatcaaa
     3901 tcagaacgta tcagcttaaa gcatttattg accgttgagg cacttactga tcaagaagtt
     3961 atgggcttga tccgcagagg gcaggaattc aagcaaggtg ctaattggac tccgcaaaag
     4021 cgccagtact ttgctacaaa cctctttttc gaaaatagta ctcgcacaca taaaagcttc
     4081 gatgtagcgg agaaaaaact agggctagaa gtgattgaat ttgaagcaag cacaagctct
     4141 gtccaaaaag gggaaacact atacgataca gtactgacga tgtcagcatt aggcgtagac
     4201 gtagcggtta tccgtcatgg tgatgagaac tattatgacg aactgataca aagtaaaaca
     4261 atccagtgtt cgattatcaa tggaggagac ggaagcggac agcatccaac ccaatgcttg
     4321 cttgatttga tgaccattta tgaagaattt ggtcattttg aaggtctgaa agttgccatc
     4381 gtaggtgata tcactcactc tcgcgtagct aaatccaata tgcagatgtt aaaacgtcta
     4441 ggcgcacaag tattcttctc aggtcctaga gaatggtacg acgatgaatt cgaagtgtac
     4501 ggacattata tgccattgga tgaactacta gatcaagttg atgtcatgat gatgcttcgg
     4561 gtgcaacacg aacgccatga tggaaaagaa agtttctcaa aagaaggcta ccatcaggaa
     4621 catgggttaa cagttgagcg tgcgaagaag ttgcaaaaac atgcaatcat tatgcaccca
     4681 gcaccagtta accgtgatgt agaattagct gattcattag tggaaggatt acagtcgcgg
     4741 atcgttcagc aaatgagcaa tggagtattc gtcagaatgg caatcttaga agcagtgcta
     4801 aatggaaaag cgtaattata ggaggatgaa aaaatgaaaa cactcattaa aaacggtcag
     4861 atcaatacac gaaaaaatat gacgacacca gcagaaattt ggatagaaga tgggcgaatc
     4921 aaagcaatcg gtacaggatt ctcagaagcc gaatttgatg aagtgtttga tgcaaaggga
     4981 caattgatca cacctggttt agtggacgtc catgttcatt tgagagagcc aggatttaca
     5041 tacaaggaga cgatcgaagc tgggactcgc tcagctgctc gtggcggttt cactactgta
     5101 tgcgcgatgc cgaatttaaa tcctgtacct gatacggcag aaaagctgag acaagtatat
     5161 gacatcatta gaaaagatgc agttgtgaag gtattgcagt atgcgccaat cacagaaaat
     5221 ctgcgaagtg agaaattagt tgatcaagaa gcactgattg aagaaggtgc ttttgctttc
     5281 acaaatgacg gtgtaggtgt ccaaacagct gggacgatgt atctggctat gaaagaagct
     5341 gcaaaaaaca ataaagccct agtagcccat acagaagatg aatctttatt atttggcggc
     5401 gttatgcacg ctggcaagaa ggcagaagaa cttggtcttc ccgggatctt aagcgtaacc
     5461 gagtcttctc aaattgctag agatctgttg ctagctgaag caactggtgt tcattaccat
     5521 gtttgccatg tttctactaa agaaagtgtc cgtgtaatca gagacgcaaa aaaagcagga
     5581 atccacgtaa cagcagaagt ttcaccccat catcttattt tgatcgacga agatattccc
     5641 gaagatttcg gcttttggaa aatgaacccg ccgctaagag gacgggagga cagagaagct
     5701 ttgatcgaag gcttgcttga cggtacgatc gattgcatcg ctacagacca tgccccccat
     5761 ggattagaag aaaaaagcca aagtttcatg aaatcaccat ttggtatcgt aggaagcgaa
     5821 acagcgtttc aactgatcta tactcatttt gtagaaactg gtcgattcac gttggaacaa
     5881 gtgatcaatt ggttggcagt taaaccggct gagatttttg gtttgaatgc aggaacgcta
     5941 acggttggtg cccccgctga tgtagcagtt tttgatatta cacaaacttg cacgatcgat
     6001 aaagaagact ttttatcaaa aggggagaat acacctttta tcggctggaa agtaaaaggc
     6061 gaaacacaaa tgacttttgt caacggaaaa cttgtgtggc agaagggaga ataaaagtat
     6121 ggaacgtttg cttatattag aagatggaac agttttcgaa ggaaaggctt ttggtgcaga
     6181 tgctagtgtc ttcggagaaa tcgtttttac aacaagtatg actggttatc aagaaactat
     6241 cactgatcaa agttttaacg gtcaaatcat tacatttacc tatcctatgg tgggtaatta
     6301 tggggtaaat agagatgatt atgaatcgat ttctccaaca tgcaaaggag tagtagtaaa
     6361 agaacatgca cgtgtagctt caaactggcg aaaccaaatg acgctagatt catttttgaa
     6421 acaaaaagga attcctggaa tatcaggtat cgatacgcgc gcccttactc gcaaaatccg
     6481 tgaagtcgga acgatgaaag ctagtatagt tgatgctgga gattccttcg aacatgcatt
     6541 tgatcagctg aaagcaaccg ttctagcaac caatcaagtg cagcaagtct caactaacaa
     6601 accttatcca agtccaggaa caggtcggaa agtcattgtc attgacttcg gcttgaaaca
     6661 tagtatttta cgagaattga gtaaaagaaa ctgtaacctc acagtacttc cctataacac
     6721 agatgccgag acgattttag cgctatcacc agacggagtc atgctgacga atggacctgg
     6781 tgatccaaaa gatgtgccag aagcaatcga aatgatcaaa aaaatccaag gaaaagtacc
     6841 gatttttggt atttgtctcg gtcaccagtt atttgcttta gccaacggtg cagataccta
     6901 taaaatgaaa ttcggacatc gaggattaaa tcacccggta cgagaagtag cgaccggaag
     6961 aattgatttt acttctcaaa atcatggcta tgcagtcgat gaagccacga ttgatccaga
     7021 aaaactcatc gtaactcatg tagaagtaaa tgacggaaca gtagaaggag tcaaacacag
     7081 agattatccc gctttcagtg tgcagtttca tccagatgct gcccctggtc cgcatgatgc
     7141 actacattta tttgatgaat ttatggaaat gatggacgca ggaaaggagc agtaaacaga
     7201 tgccgaaacg tacagatatc aagaaaatta tggtgattgg atctggtccg atcatcattg
     7261 gtcaagccgc agaatttgac tacgcaggaa cacaagcctg ccttgcctta aaagaagaag
     7321 gatatgaagt ggttttagta aattctaatc ctgcgacgat catgacggat aaagaagtgg
     7381 cagatcaagt atatatcgaa ccgatcacac ttgaatttgt atcaaggatt cttcgaaaag
     7441 agcgtccaga tgctctcctt ccaactttag gcggacagac aggcctcaat atggcaatgg
     7501 agctggcagc atcaggtatt ttagaagaat tgaacataga acttctagga acaaagcttt
     7561 ctgcgatcga tcaagcagaa gatcgtgatc tattcaaaca gttgatgaaa gaattgaatc
     7621 agccaatccc cgagtctgaa atcgtcaata cagtagaaga agctgtagat tttgcaaata
     7681 aaatcggcta tcctgtcatc gttcgtccag cctttacttt aggcggaact ggtggaggta
     7741 tgtgtgaaaa tgaagaagaa ttacgtatcg tatcagaaaa tggattaagt ctttcccccg
     7801 ctacccaatg tttgattgaa cgaagtattg caggttttaa agaaattgaa tatgaagtga
     7861 tgagagattc ggctgataat gcaatcgttg tctgcaatat ggaaaatttt gacccggtgg
     7921 gtatccatac aggtgattct atcgtatttg cccctagtca aacattgtct gaccatgaat
     7981 atcaaatgct tcgtgatgct tctctttcga ttattcgagc acttaagatc gagggaggct
     8041 gtaatgtcca actcgctctt gatccacata gttttaatta ttatgtgatt gaagtcaatc
     8101 cgcgtgtttc tcgttcatct gcgttagcaa gtaaggcgac tggctatccg atcgctaagc
     8161 tagctgctaa aatagcgctt ggattaacat tagacgaaat gaaaaatcct gttacaggaa
     8221 cgacttttgc agaatttgaa cctgcattag attatgttgt agctaaaatt cctcgctggc
     8281 cgtttgataa gtttgaaaac ggagaacggc ttttaggcac gcagatgaaa gcaactggtg
     8341 aagtgatggc tatcggtcgg aatatcgaag agtctttatt gaaagctgtg cgttctttag
     8401 aaatcggtac acaccatatc gaacttcctg agttggcaga catcacggac gatgacctaa
     8461 tccaaaaagt ggttcgagca caagatgatc gcctgtttta tgtagctgaa gctattagaa
     8521 gagggtacac catagaagaa ttagcggatt tgacaaaaat cgatttattc tttttagata
     8581 aactgttgca tatcgtagaa atcgaacagc agttgaaaca gcagccgttg gatgacaaga
     8641 tattgaaaac agcgaaacaa aacggtttta cagacaaaaa agtggcggag ctgtgggaaa
     8701 taaacgaaga agccgttcgc cagatgagaa aagaaaatga tttgattcct gtatataaga
     8761 tggttgacac ttgtgcagcg gaattcgaat caaaaacacc ttatttttat agttcctatg
     8821 aatatgaaaa tgaaagttca cgatcagaaa agccctcaat tctagtactg ggatctggtc
     8881 ctatccgtat cggacaaggg gttgaatttg actatgcgac tgtgcattct gtcaaagcaa
     8941 tccagcaggc aggatacgaa gcaatcatca tgaacagcaa tcctgaaaca gtttcgactg
     9001 acttttctat ttcggataaa ttatattttg agccattgac cttcgaagat gtgatgaatg
     9061 tgatcgagct agaacagccg ataggagtaa tcgttcaatt tggtggtcag acagcaatca
     9121 acttagccga acctttgact gctgctggtg taaaaatctt agggacaacg atcgaagact
     9181 tggatcgtgc ggaaaatcgt gatttattcg agcaagcgct gaaagagttg gatattcctc
     9241 aacctttagg ggatactgct acaagtaaag aagaagccgt ggttatcgct tcgagaattg
     9301 gttatccagt tcttgttcgt ccaagctatg tattaggcgg acgtgcgatg gaaatcgttg
     9361 aaaaccagcg agatttagaa gattacatgg aacatgctgt gaaagcatct ccagaacatc
     9421 cagtactagt ggaccgttac ttgcttggtg gtgaatgcga agtggatgcg atttgtgacg
     9481 gtgaaacagt actcatccca gggatcatgg agcatattga gcgtgctggt gtccattcag
     9541 gggattcaat ggctgtgtat ccacctcaaa cattatctga atcgatcaag aaaacgatcg
     9601 aagattacac gatccgctta gcattaggtt tgaattgtgt cgggatgatg aatatccagt
     9661 ttgtcatcca tgaaaatgag gtttatgtga tcgaagtcaa tcctcgagca agtcggactg
     9721 ttccattctt aagtaaggtg acaggaattc caatggcaca aattgcgacg aaagctattt
     9781 tgggagagaa actaaagaca cttggttatg aaaacggact ttatccagaa acaaaagaag
     9841 ttcatgttaa agcacctgtc ttttctttta cgaaattgca gaaagttgat acttacttag
     9901 ggcctgaaat gaaatcgact ggagaagtaa tgggatctga cagacaatta gacaaagctt
     9961 tgtacaaggc attcgaagct tctggattac acttgcctga atatggagcc gtattattta
    10021 cgattgcgga cgaaacaaaa gaagaagcac tagaattagc taaacgtttt acagaaattg
    10081 gctatagtct gataggaacg aaacatacag ctgcttattt cgaaaaaaat ggtctggtcg
    10141 ttgaaacagt agcgaaaatt tctgaggaag cagcagaaaa aaatgtagtc gacttaataa
    10201 gagatggtaa aacacaagtg gttgtcaata cgattgataa agatcgggga aatgcttcta
    10261 aagacggttt catcatccga agagaagcag ttgaacatgg tattccattg tttacttctt
    10321 tagacacagc agatgcgatc gttcgagtga tggaatcccg tgctttttca actaacccta
    10381 tttaacggcg agcacatgtt aagacaaaaa gacgaaaaac cgttgcagga ggggacagat
    10441 gaaacaagaa atgatggaaa tcgttcagca aaaacagctg gcaccaaaga tttttgaaat
    10501 gacattgaaa ggtgatctag taaaagaaat gcagcaacca ggacagttct tgcatattct
    10561 cgtgccgcgt gcagacttgc tattacgccg accgattagc ttgaatcata tcgaccacga
    10621 aacaaatact tgccgtatca tttatcgggt ggaaggtgag gggactagag tcttctctac
    10681 tctttctgcc ggagaccatt tagatgtcat gggtccttta ggcaatggtt ttgatttgtc
    10741 caatcttcat acaggagacg aagtatttat aatcggtgga ggaatcggtg tccctcctct
    10801 atatgagtta tcaaaacagt tagtcgaaaa aggaattaag ccaacgcatt ttttaggttt
    10861 tgccacacat gaagtcgttt attatgaaaa agaatttaat cgtctaggag aaacaagaat
    10921 cgctactgac gatggaacat acggtatgca cggaaatgtc ggtaatttat tgttaggaac
    10981 gaagacggaa cctacagctg tttttgcatg tggttcaaac gggttattga agacagtgga
    11041 acagctgttt tcttcgcatc aaaatgtcca gttatcatta gaatcccgca tggcatgtgg
    11101 gataggggct tgctatgctt gcgtttgtca tacgccggaa gatgaaacag gaacaaaaag
    11161 tgtgaaagtt tgtgacgaag gaccgatttt taaaatagga gaggtaatta tatgaatgag
    11221 aatcctttag cggtcaaact tcctggccta gaattaaaaa atcctatcat gccggcaagc
    11281 ggctgtttcg gttttggcaa agaatacgga aaatactatg atttgaatca gcttggatcg
    11341 atcatggtca aggcaacgac tccaaatgcc cgctttggca atcccacgcc aagagtggcg
    11401 gaaacaccat ctggcatgtt gaatgcaatt ggtttgcaaa atcctggtct agacgttgtt
    11461 atgcataaac ttcttccaga attagaggag tacaatgagt tacccatcat cgcaaatgtt
    11521 gctggtgctt gtgaagaaga ctatgtagaa gtttgtagca aaattggcga agcaccaaat
    11581 gtgaaagcaa tcgagctgaa catttcttgc ccaaatgtca aacatggcgg aattgctttt
    11641 ggaacagatt ctgatgttgc ttttcaatta acacaagcag taaagaaagt ttcttctgtt
    11701 cctgtctatg tcaaattgtc cccaaatgta acagacatcg ttccaatcgc tcaggcaatc
    11761 gaagctggag gggcagatgg gtttacgatg atcaatactc ttcttggtat gcggattgac
    11821 cttaagacaa gaaaaccgat tttagccaat caaacaggtg gactctccgg accagcaatc
    11881 aagcctgtcg ctattcgttt gattcaccaa gtagcaagta tatccagcct tccaatcatt
    11941 gggatgggcg gcgtgcagac agtggatgat gttcttgaga tgtttatggc tggagcaagt
    12001 gctgtagcta tagggaccgc gaatttcacc gatccgtata tctgtcctaa actaattaaa
    12061 gagctccctg tgcgaatgag cgaactaggc atcgaatcgc tggaaagatt aatcaaagaa
    12121 gtgagagagg aacgtgaacg atgaatcaac tagaccaacg accaattatt gcattagatt
    12181 tttcaactag gcaagaggta gaagactttc ttcgattttt cccgaaagaa gaaaaattgt
    12241 ttgtgaaaat cggaatggaa ttgttctacc aagaaggacc tgaaatcgta cgttatctaa
    12301 aagaggctgg tcataatgta tttttagatc tgaaacttca cgatattccg aatacggtcg
    12361 aaaaagcaat gcggggattg gcaaagcttg gtgttgatct cacctgtgtt catgccgctg
    12421 gcggtattcg tatgatggag gcggcaatgc gaggattaga agaggggact ccagaaggtg
    12481 gaaaacgtcc gctacttcta gctatcactc agcttacttc tacaagtgaa gaagaaatgc
    12541 atgccgatca actaatcgaa gtttctttag aaaaaagtgt gatccattat gctagctgtg
    12601 ccaaaaaagc aggattagat ggtgtggttt cttctgcttg ggaagtagaa gcgatcaaag
    12661 aaatggcagg agacgaattt gtttgcttaa ctccgggtat aagaccagaa ggaacagtcg
    12721 caggagatca gacaagagta gtcacacctt ctcaggcaaa aaagatcggc tcaactttta
    12781 tcgttatagg tcgaccaatc actcagtcaa ctaatcctta taaagcatat caaacgattc
    12841 aaacagaatg gagctaacct aaaatgaatg ttgaacaaag tatcgcaaaa gacttattag
    12901 aaattgaagc agtattttta aatccaagta atccttttac atgggcaagt ggaatcaaaa
    12961 gcccaattta ctgtgataac cgaatcacga tgagctatcc aaaagtccga aaagagatcg
    13021 caaaaggatt agcaagcaaa atcaaagaag cttaccctga agtacaagtg attgctggaa
    13081 ctgcaacagc aggaatccct catgccgcat gggtagcgga aatactggat ttgccaatgg
    13141 tctatattcg aagtaaggcg aaagatcatg ggaaaggtaa ccaaatcgaa ggacgtatat
    13201 ttgaaggtca gaagatggtt gtgatcgagg atttgatttc tacaggcgga agtgtcttgg
    13261 aagcagctga agcagcaaaa agagaaggtg cggatatcct tggtgttgcg gctatcttta
    13321 cgtatgagtt gccaaaagga aaagcaaatt tcgaaaaagc cgaaatccct cttatgacgt
    13381 tgaccaatta ttctgtattg attgaagcag ctttagaaga tcgttatatc gatgagcaag
    13441 aattaactct gctaaaagaa tggaaaaaag atcctgaaaa ttggcagatt taagcgcgct
    13501 tgatgaagat agagaaatcc gatttgtgtc tccagaatca tcaattggaa tagcaaaaat
    13561 ccaaaaactg attgcgagaa gcatcttttc atcaggtatg aaaagaaact tctatcgtga
    13621 tcagtttttg atttttttaa aattcatttt tctaagactg gttagcagaa agaagagaat
    13681 aatctcttct cttttttcta ttatatatag attttctcta ttttttggta agggagctgt
    13741 ttcaaaaatg gtaaagagcg gaaatgttgc gaatatcagt tatttaatga tatgatattc
    13801 ggaaaaagaa atctcaaagg gaggtaactt ttatggggtt gaatgaattt attttaggat
    13861 tgaacaattt agagacgatc acaagggcac ctggattttt taaatataca gagcatacag
    13921 ttgcagcaca ttcgtatcgt gtagcatcta ttgcgcaagt tctaggagac attgaagaag
    13981 cgaatggaca accgatcgat tggaaactgc tctatgaaaa atcattgaat catgactaca
    14041 cagagcgttt tattggtgat atcaaaacgc cggtgaaata cgcaagcaaa gagttaagag
    14101 ggatgctgca aacagttgaa gaaaagatga cggacgaatt catcaaccaa gagattccag
    14161 ctgagttcca agaagtttat cgccgtcggt tgttcgaagg aaaagatgaa accgtagaag
    14221 gagaactatt agccatttca gataaggttg accttcttta tgaatctttt gatgaaatca
    14281 tcaaaaataa cccggaaaaa gtttataagg agatgttcat tgaggcagtg actacgatta
    14341 aaagctttga acatcggcct tctgttcagt atttctttaa agagatattt ccggaactat
    14401 tgaaccagga attttatgga aaagaggcgt ttttagaaac cgtctcaacg attgtaaaga
    14461 aagaagaaaa ataaaaatga tatgagatgc agggggaagt atagttgaaa aagaatatgg
    14521 atgtcgaatg gggctattct ggagacaaag gtccggaaaa ttgggcgagt ttatgtgatt
    14581 ggtttgcgag aggtgcggaa tttccattgc agtcgcctat tcatttgacc aagggggaag
    14641 aaaccaaaat agaaggagac acgctttcct ttcattatca aaagcaacgg tttactgaca
    14701 aggaattcaa aaacacgatt catttagtac cttttgacca actcagttat gtagagttca
    14761 agaaagaacg ctattatttg acagatattc atttccactt gccttctgaa catatcatca
    14821 acggaaaaca ggaagatatt gaatttcatt ttgttcatat gaacagcaaa aaagagaatc
    14881 tagttgtggg tgtcatgtac cagctaatgg tacaggaagg ttggctgtat aatcaagaaa
    14941 atggggaaag ttggaactta gaaaaacatg agcattgggt aaatccggat gtgttctttc
    15001 cggagcaaaa aagccatttc cattatattg gctcattgac aactccgcct accagcgggc
    15061 caatccaatg gtttgtaatg gatcatgtag gaaaactgaa ccaagagttt cttctcccct
    15121 tcgatggtca gtatgctcga ccgaacaacc gaccaatcca gcccttgaat ggaagagaaa
    15181 tttattattt cgaggagttt gagcaatgaa tattcagcag atgaaatacg tggtcgctgt
    15241 agcaaacaat ggtagtttca gagaagcggc aaagaaaatg tttgtctccc agccaagtct
    15301 ttcagctggt atcaaagaat tagaaaccga gttagggatc acgctgttta ctcgaacgaa
    15361 tcgaggggca tatttaacag aagaagggca agacttctta aatcgtgccg agaaaatatt
    15421 ggtccagctt gatacattag aaactcacta tctaacgagt gaaaagagag acaatttttc
    15481 gatcgctgcc cagcattatg atttcttagg tcctttgacc gcacagctac tcgaggaatt
    15541 caaagacgag atcaaacaat ttcggatcat ggagacgaca acagcgagag tgatcgaaga
    15601 agtcaaagag cagcacagtg agatcggcat tatttatatg aatgagcata atcaagctgg
    15661 tatcagaaga tatttacaac aaggagagct aagtggccgc agcattggaa aattcaaaac
    15721 gcatattttt ttaaggcaag gacatccatt agctgaacac gcagagatca ccaaagaaga
    15781 attgttatct tatcctcaag ttcgttttac acaggatgga aataatttcc cgtattttta
    15841 tgaagacctt atcgagatcc ctgatcaaga aacagtgatt tatacgagtg atcgaggtac
    15901 tttgatgaat atcgttcttg aaactgatgc ttacgcttca ggatctggta ttgtgattgg
    15961 agggattcgc gagcacttgc ggttaatccc tttggcagaa ggcgaactaa atgaatttta
    16021 tatcatttat tcggcgaaac gtccgctcag tgaaatcgct caaagattta tcaaagagct
    16081 tactcggtta ctggaccagc agagctaaga aaagcagatg agaacgctgt ttattgaaca
    16141 ccgttgatca aaaaagaggg gttgtgacag aattttgtca caactcctct ttttgtagca
    16201 aacaatttat aactaattgc tgttgtggga aagtacgcaa atatgattct agaaacactc
    16261 attgattacc tgttttgttt catagctgta attttttctt cttctggtgt cacgataatc
    16321 gtcttttggt tttcataaat aacataacca ggcttggcgc catttggttt tcggatatgc
    16381 tttacctgta caagatcaac cggcacttgg gcagagtatc ggtattttga aaaataggca
    16441 gctaactcgg cagcttctgt aattgtttca tctgagggct tgtcactttt aatgatgacg
    16501 tgcgaaccgg gtatattttt tgcatgaagc caatagtcgg tttttttagc tgtcttcatt
    16561 gtcagctggt cattttgaag attgttttta ccaacaagaa tgagcgttcc gtcagaagaa
    16621 aagtattgat caggctgaga tggtttctta cgtttttgtt tttttaagtt cttctttttc
    16681 aggtatcctt ctgaagtcaa ttcttcttta attgcttcga tatccattgg gccggcaatt
    16741 tccagttgag ataagacaga ttcaagatat tggatttcat ctttggcttc ttggatttgt
    16801 tcgccaatca gcttaacagc gttcttcagc ttttggtatc gatggaaata tttctgtgca
    16861 ttttgatttg gtgtcaatgc cggatctagt gctattttga ttggccgatc ttcttcataa
    16921 taattaggta aaacaacttc ttttgcgccg cgtggtactt gtgtcagaaa agtagtaagt
    16981 agttcgccat ttctacggta attctcagcg ttttcggatt ctttgagtgt ttgttcacgt
    17041 tttttcaatt tatttttatt tcgttttagt tcattttcga tttttcgaat cagttctccg
    17101 ccttgttgtt ttgcccgatc tttctctgct ttttcgtgat aatagctatc taataattga
    17161 ctgagtgttg gataagctgt tacagcagat gcctgttcgg acaatacctg ataagcaatc
    17221 ggtgtgaaaa aatctttatt ttccgtttcg taaaaagtag gaataggctg atggctgatg
    17281 gctgagaaaa attcgtccca tacaaccatc ttttcattag gacgttccag taatcttttg
    17341 gtcagctctt gagcgatatc gaaaccgatt ccctgaaact gacgttggat tgcttttgga
    17401 tctagttcag tttgggaaag ccgctgaaag attttttctt tctcggagct aaatgggttc
    17461 aagacctctt gttttggcgg agcaacatat tctacaccag gaagcaaaga acgataagtg
    17521 ttttgagagc ttccaatgtg tttgatcgca tccagtattt ttccagtttc tcgattaact
    17581 aagacgatcg tactatgacg tcccattaat tcaacgatca atatgatatt ttgtagatca
    17641 cccaactcgt ttcgtttagc aaagtggaaa tggatcactc gatcattttc gatttgttcg
    17701 atgtcttcta aaatagcgcc atccaaatat tttcgaagca tcatcacgaa gttaggcgga
    17761 ttatcaggat tctgataatc gatttgggta atctgtacac gagcataact tgggtgcgca
    17821 gataataata atcgatggtt tttgccgcgt gaacgaatca ccagcacgat ttcattttca
    17881 taaggctgat ggattttcga gatccggcca gatagtaacg tttcacgcaa ttcgttgacc
    17941 atcgcgtggg taaatacacc atcaaaggac atggttcatt cctcatttct tttcataagt
    18001 atattcatta taacgaaaaa aagaagaaaa accaatttgg ccgaatagta aagcaaaagt
    18061 ctagaaaaat cttggactaa ttacataaaa aacagtgttt ctatcgttga aatccaaatt
    18121 tttaggtata ataaaaaata ggagaaaaag tagaaaagaa ggtgtttgtt atttgggaag
    18181 tagttaaatc gagagtacaa gaaatgaatg aacatttctc agtgtcattc gcagaaaatc
    18241 caagtaagta ttttcgacgt cttgtttttc tgctcatctt atcattgctg ttaacagttg
    18301 caatgttttt attgccggaa aaaatccgag aagagcaagt aaatagtctc gtttattcgt
    18361 ccataccaac cagcaaattg aaaccttttg attacagcaa agctgatcag aaaatcaaac
    18421 aaagccgggc gatatcggtg atgttcagca aaccgaacgg gaaaacctat caggacatgc
    18481 ttgatgtttt taatgatcct gagcttatgg aagaattgaa tcgaccgatc ttttattatc
    18541 cgatcgttta tgatgtacat gaactggaac aaaaatataa catacgaacg gatgaagtga
    18601 catttatttt ctttgatgga ggaaaagaag caaatcggat aactgcgggt aagggaggaa
    18661 taactgattt tgataaggag ttgatccctg aactgaatcg acttccgtta gccaatatca
    18721 aacaattaga agaagaattg gatagaacgg gagtaacttc ccaaaattcc gagattgcta
    18781 attgagacat tggcgcgatg aaacggtggg tctatatagc gctattttta attatcagaa
    18841 caagtgatgt ttgatcttca aaataacaaa taaaaaatta aaaaatatag ttgacgaaat
    18901 tcaaaaattc gattagactt aaatcaattc ttaaggaaag ggtgacctgt aatgaactta
    18961 tcaaaacgta tttcagcaga aaacaaaaaa aattggcgca gctggcgtag ataagttgta
    19021 ttaatggtga gactttccca tagatacaac tttttagagt gcatttctaa agcttgtatc
    19081 tatgctagac atgaacattg actgccgcat agattagaat ctatgcggtt ttttgtgtaa
    19141 agacaatgac cgctttggcc attgtctttt tttgtagact ggatacttgc ctgattataa
    19201 taaataaaaa gattgacttt gcaggaggaa aaaaatgaaa aacaaacgat taataacagt
    19261 tgtagtaatt attttagttt atttagcagg tgcatttatt tatggaaaag cctcaggcga
    19321 aaaagaaagt acttcgacga accagacaaa aaaaacaaca gttgttgggg tattgcaata
    19381 cgtgagccat ccggcattag atgagattta tcgaggaatc aaagatggtc tgaaagaaga
    19441 aggattaaaa gaaggagaaa atcttgagat caaatttcaa aatggacaag ccgatcagag
    19501 caagctagcc acaatgagtc aacagctagt acaatctgat ccagatgcct tgattggaat
    19561 cgcaacacct gccgctcaat cattagcaaa cacgacttca tcgatcccag ttgtgttagg
    19621 tgctgtaacg gatcctgtgg gggctggatt agtcgcttct ttagagaaac ctggtggtaa
    19681 tgtaacaggt gtttctgatc agccgcctgt tacttcacaa atcaagcttg cttctgattt
    19741 gttgccaaat gcaaaaaaag tcggaatctt gtatgcttct tcagaggata attcaaaata
    19801 ccaagtagca caagcagaag aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc
    19861 ggtgccttcc actaatgaga ttgcacaaac ggttcaagtg atgagccgac aagtcgattt
    19921 tatctatgtt ccattagata ataccattgc caatgcgatg caaacagtgg taaaagaagc
    19981 aaacaaagca aatatccccg tcattccatc tgtggataca atggtggaac aaggtggact
    20041 agcaacaatc gggatcaatc aatatcaatt agggcttcaa agcggtaaga tggcggcaga
    20101 acttgcgagt ggaaaagaaa aaccggaaac gacgcctgtt tatacatttg atcaagggga
    20161 tacagtgatc aatcaatctc aagctgatcg cttaggaatc attgttcccc aatcgatgaa
    20221 ggaaaaagcc aaaatcatta cagatgactc gcagcaagaa acatctaagg aggatgacaa
    20281 atgatcgtat cagctatttc acaaggcatg ctttgggcaa tattaggatt agggattttc
    20341 atgacttatc ggatcttaga ctttcctgat atgacgacag aagggtcctt cccattaggt
    20401 gctgctgtct gtgtgacagc tattactcat ggggtagcgc cgattgtagc tacattatta
    20461 ggcgtaggtg ctggcatgat agcaggactc gttacggggt tactttatac aaaaggaaag
    20521 ataccggtta ttttagcagg gattttagtg atgtcaggat tgaactcagt tattttgttt
    20581 gtgatgcgaa caccaaatct ttctttattg aatcatccga ttctacaaga cagcttccat
    20641 cgctttgggt tacctgatta ctttgacaca gtccttcttg gcttcatctc tttgggcatc
    20701 ttgattgctg tactgctatt cttcttcaat acagatttgg gtcaaggcta tattgcgaca
    20761 ggagacaacg aaacgatggc tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta
    20821 ggtttaactt tgtcgaacgg tgtcattgca ttatccggtg cgctgattgc ccagaacgac
    20881 ggatatgcag atgtaaataa agggattggc gtaatcgtca tcggtcttgc ttccatcatc
    20941 atcggtgaag tgatcttcca agaattgacg ctagcagaaa gattgattgc tattgttgtc
    21001 ggcagtatca tttatcagct attgatttta gtagtaatca aactagggtt tgacacgact
    21061 tatctgaagt tgttctccgc tatcatctta gctatttgtc tgatgatccc gcaattgaag
    21121 aaagcactga acttaaaaac gggttttgag aaggaggcat agtaatgact gatatattga
    21181 caatcaaaaa tggcagaaaa gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg
    21241 atgaaattaa ccttacaatc agagaagggg acttcatcac tgtccttggc gggaatggtg
    21301 ctggaaaaag cacactgttc aatacgatcg ctggtacgct tcaactcagt caaggatcga
    21361 tctcttttaa agatcagctg atcacaaaag attcagaaga aaaaagagca cgattccttt
    21421 ctcgagtttt ccaagacccc aagatgggaa cggctcctcg gatgacagta gcagaaaatt
    21481 tgctacttgc acaaaaaaga ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc
    21541 aaaaagaaga ttttttcaga ctatgtcaag aagtcggtaa tggcctagag caacacttgg
    21601 atactccagc gggtgaatta tctggtggtc aacgccaagc attaagcttg ctgatggcga
    21661 cgatcacagc tcctgagtta ctattattag atgaacatac tgcagcttta gatcctaaaa
    21721 cagctaaagc attaatgcga ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc
    21781 tcatgatcac gcatcgaatg gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc
    21841 aaaaagggaa aatcgttcaa gatatttctg aaaaagaaaa gcaacatttg acaatgggcg
    21901 accttttgca gttctttgaa gaagtagtgg attgatttcg acagaaaaat tcctttcagt
    21961 agaaggactt gaaaactcat tgacatcttc ctttgattca tcctatactt aaaacaataa
    22021 acaattaaac atatttgggt gttttgtagg atcacgtagt caatgatttt aacaattaaa
    22081 tagaatattc tttgctctga caagtaaacg atgagaaaag aatttgctac tttgccacct
    22141 agacgtttcg tactttttcg tatcaaacgc taagtggctt tttttattga agtaagaaca
    22201 aaggaggaat aagatgattt ctggacaaac caaattagct gggctctttg ctgatccggc
    22261 ttctcacagt ttatccccat tgatgcacaa tactgctttt gaagcaaaca acatcgatgc
    22321 ggtgtattta gctttccaag taaatccaat gaatttgaag caagcagtcg aaagtattcg
    22381 aacttttgat atgttaggtg taaatctttc gatgccgaat aaaacagcgg ttattcctta
    22441 tctggatgag gtaagccagg aagcgcaatt gatcggagca gtgaatacga tcgtgcacga
    22501 aaagggtcgt ttgattggct ataatactga tggtgcgggc ttcatgcaat ccgtgcaaga
    22561 agctgggatt tcgatcaaaa agaaaaaaat gactctactg ggagctggag gtgcagcaaa
    22621 ggcagtcact gttcaagcag cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa
    22681 caaccaaaca ttcgctcaag taaaagaaac attcgaacaa ctcagctatc gaacttcttg
    22741 taagataaaa gttcgtgact ttgccgatac gcatcaatta agaaaagact tgaaagaaag
    22801 ctgtcttcta gtcaatgcaa cagatatcgg gatgggaaag aaaatcaatc agtcaccgct
    22861 cttagatata agcatgatgc atgagaatct agcggttttt gatttgattt atgcacctag
    22921 agaaacacgt ttattaaaag aagcaaaaaa agtaggagca aaaggataca atggacttgg
    22981 catgttgatc taccaaggag caatcgcgtt tgaattatgg acaaaggaaa agatgccgat
    23041 ccatgtgatt gaaaagctgt ttgattaaaa aaggataata gataaagatc ttttattctc
    23101 gcataaacgg tctcaagaaa agtaatcctt tatgtatcga agcatttatt tcgttatttt
    23161 ttattaaatt atcaaaaaat gataggcttt tgtcgattgg ttatcgtatc ctttatttat
    23221 cagtaagaaa cgaggaggaa ccacaatgag tcggatggat cgctataagg acatccataa
    23281 gaaggcgaaa cctctgaagc gggatacggg gagtattttt gctagaagag aacgaaaacg
    23341 aacgcgtatg gaaaatgagg aagatgctac ccgtatttat gaaagagagc agcattcatt
    23401 tgaacatttg aaccaatacg agcaacagat atacagagat gcaccaagag cagaagaaca
    23461 acaaaaaaat aaaaaatttt tcaatcggca aaagaagaac aaaccgttaa aacctcgaag
    23521 aaagcgatcg tggaaaaaaa tcattctatc gattttgctt gttttgatcc tttattcagg
    23581 tatctcattt tttattggta aatatgtagc tgaacatgac acaagcttag aggcagcgac
    23641 ttcagaaacc tttcatgggc aaaaagcgac gaatggtgcg cataatattt tgatccttgg
    23701 aagtgataca cgcggagaag atgccgggcg agccgatacg atcatggtct tacagttgga
    23761 tggtccttcc cataagccga aactgatttc ttttatgcgt gacagcttcg ttacgatccc
    23821 aggggtagga caaaataaga tcaattcagc ttatgcatac ggaggagccg atctagtgcg
    23881 tcaaacttta gtagaaaatt ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa
    23941 atcgtttgaa aaagtgatcg atgccttatt catgaacgga gtgaagatcg atgcagaaaa
    24001 agatctgaat ctggatggtg tagatattaa aaaagggggc cagaagatgg atggacatgt
    24061 tcttttgcag tacgcacgtt tccgaatgga tgaacaagga gactttggcc gtgtcagacg
    24121 ccaacagcaa gtaatgaacg ctattttcag ccaattgaag aatccattga atctgatccg
    24181 tgcaccttat gcagctggta aagctttagg atatacatcc acagatgtca ctgcttggtt
    24241 cttgacgaaa aacctctttt cgattgcacg aggagctggt ggcattgatc gaataagtgt
    24301 gccagtagac ggttcgtgga attatgggca aagtgattat gctggcagta ttcttgaaat
    24361 tgataatcaa atgaatcaat ctgctattac agattttttg aataaatgat cataaaaaag
    24421 atcaagaaga agctgcgcaa ggcttttgtc atgaatcaat caaacgattc actcgtaaaa
    24481 aacgctttct tatggtagat ttaaccaaga gaggagctgt ttacattgga tatctatctg
    24541 aacttcaaaa atgagtcgcg tgaagccatc gctttttatg aaaaagtttt tcagactgaa
    24601 gcatcaggta tcaccacata cggtgaaatg cctggaatag aacaaatgaa cttgccagaa
    24661 gaagcaaaaa atctaatcac gaatgcacgg ctgaacattt atgatacatt agttatgttt
    24721 gcagatacgc cagaaggaat tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc
    24781 atccagacaa gaaatgaaga agaaacgaag cgcctatttg aaagcttagc tgagggtggt
    24841 aagattgaga tgccactgga tgctgttccg tgggctcagt tgtatgggat ggtaactgat
    24901 cgatttggca tcaactggca gattaaccaa gagtgacagc taaagatgaa tattttctgg
    24961 aaacatcaag cagcaaacgg atcatcgttt gtttgcttga tgtttttgtg tctatttcga
    25021 ctatacggta tctttttttt attgccagat gcgaaaatat gttccataat agagaagtcc
    25081 gcaaaaaatt aagtaacgga ggtcagaaga tgaagacggt gatactagct gaaaaacctt
    25141 cacaagcaaa agcctatgcc gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg
    25201 aaatcagcga tccattgttt gctggtgaag taaccatcac ttatggtttt ggacatttgg
    25261 tagatatggt tgctccagga gcttatgaag agcgttgggc aaaatggtct ttagaaaatt
    25321 tgcctatttt cccagaaacc ttccgttacg aagttccaat ggacaaacgt gcacaatttt
    25381 cgatcgtaaa acgagaattg cagtcagccg ataccatcat tattgcaaca gatggtgatc
    25441 gagaaggtga agccatcgcg tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa
    25501 agaactattt acgtttatgg atcaattctc ttgaaaaaga agcaatctat gaaggtttta
    25561 agaatctcca acctggaaag aactattttc caaaatataa agaagcgcaa gcaaggcaga
    25621 acgctgattg gctgattgga atgaatggaa gtccattata tagcttgcta cttcaacgaa
    25681 aagggattga tggcagtttt tctcttggtc gtgtacaaac accgacactc tatatgattt
    25741 atcagctgca agaagaaatc aaacacttca aaaaagaaac gtactttgaa ggagaaggtt
    25801 taattacgac aaacaaaggg cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa
    25861 cccaagaaga gttaacgaag gaaatagaaa aacttggagc gcatttaggt aagcaaaacg
    25921 gacggattct tgaagttacg aaaaaggaaa agaggttgca tagtccacgt ttattttcgc
    25981 tatctagttt gcagacaaag atgaatcagc taatgaaagc aagtgccaaa gctacacttg
    26041 atgctgctca aggattgtac gaaagcaagt ttctaagcta tccaagaacg gattcctttt
    26101 acattacaga aaatgagcat cagtatctgg ttaaacatct gcaaaattat aaaaaatttt
    26161 taggaataga agaagtacag acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga
    26221 aaaaagttca agaacatcat gcaattattc cgacaaagac gattccgact cctgcacgct
    26281 ttgcgaagct ttccaaatta cagcaagcaa tctatttgca agttttgcgc actactgtgg
    26341 ctatgtttgc tgcagattat atctacgaag agacgaccgt aatgacgggc gtacagcaat
    26401 taaagctaaa aagcatcgga aagatcccat tacagcaagg ttggcaggaa atcttacgca
    26461 tcaagtcaaa aaagaaagaa gctcagactc ttcctcctat cgtagaaaat gaagaagcag
    26521 aagtagaact gaaaagtatg gagaaagaaa cacaacctcc aaaaccctat accgaaggaa
    26581 cgttgatcac agcaatgaaa actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa
    26641 ttctcaaaga agtagaagga atagggacag aagctacacg agccaatatc attgaaaatc
    26701 ttaagcagaa gcattacatc gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa
    26761 tcactctttg taaggcagta gccaatgaac cattactgac gagcgcagaa atgactgcgc
    26821 gctgggaagg gtacttgaga aaaattggta aacaagaggg gacaccatct gtttttttag
    26881 agaatatcaa aaaattcatc ctacatttat tggcagaagt tcctaatcaa atcgacaagg
    26941 tagacttttc agatgagatt agcgggacac gtcagataaa agcgatggag aagaaaaacg
    27001 atcaattagg aagatgtcca aaatgtaaaa agggaatagt catgctttat cctaaaatcg
    27061 ctacatgctt gaatccggaa tgcgacttca aattgtggcc aacagtagct aaaaagaagc
    27121 tgactaaaac gaatctcaga gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag
    27181 gttttactgg taaaaaaggg aaattcgatg cagttttagt attaaaagaa gatatgacaa
    27241 tcggtttttc ttttccatgg atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag
    27301 aaacatcaag aaaaaataat gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga
    27361 atatttattc cgaaaagtga gaatataaat accaataaaa ctaaaaaata tcagtgaatc
    27421 aagcaaaaac gttggggtag gctgatttta tatagatagc aaggagtttt tttatttgaa
    27481 aaaagtgcat tcatttttaa attatcttat gggagcaatc acgattgtgg ctgtgtacca
    27541 agcatttcta aagtatcctg attacgggac gaagttgttg tcattgacgc acaatcaaac
    27601 agcatattca ccaacgttga ttttatcgac tttgactgca ggagctaaaa gcgctactta
    27661 tttttttcat aaaacgacaa gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt
    27721 ggtggtagcg gctatgatca ttatttattt tctaccagtt attacacaag tatttcatca
    27781 atattttggt taagaatacg agagtttgtg acaaaagtca tgtcacaaac ttttttcttt
    27841 gtcatttttt cgtaagaaaa agcaaaataa aagtatatac attgattaaa agtatatact
    27901 tggatataat gtacttacta tagaatgaga atggtataga gggtggcgaa atatgaaaaa
    27961 aatgaaaaag tataaaatta gaaaatctgg caatagcgat gtaacaacta tcccaccaga
    28021 agtaaaagaa ttcatgggtg tgcagacagg tgacgcaatc agttatgttt ttcaatctga
    28081 tggttctgta cgaatgatca aagcacaaga agaacctgat attgattcat tagttgattc
    28141 gatcatgaat caatatgaag acgcgttaaa agatttggtt gatttatgag gtatctttcg
    28201 actaaagaaa ttatcaaaat caacgcgaaa gttattttac cgtcactcgc caggcgaaat
    28261 gatcggtgta aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt
    28321 attcaatcaa gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt
    28381 agctaaaaaa catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt
    28441 gtttcttcaa ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat
    28501 cataatgatt accactagtt cattggattt cgattcacta aagtccaaga taaccagtta
    28561 tttacgacaa accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc
    28621 atgtcattaa ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata
    28681 aaaatatgga aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt
    28741 gaatagtagg aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac
    28801 ataatatata ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt
    28861 atcaaaaatc gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc
    28921 agtttttctt actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt
    28981 taatcaaata tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt
    29041 taattaacca cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt
    29101 catatgttca tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt
    29161 tgcttaccta ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt
    29221 tttaggattt ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta
    29281 aagcacttgt tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa
    29341 cttcacgaac accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt
    29401 cgtattcacc agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag
    29461 cttttacgat acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa
    29521 tgatctcata agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa
    29581 tacgatggtc tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc
    29641 atgcagcaac ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt
    29701 caacttttaa tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag
    29761 taccgataac acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat
    29821 ctactgggtt tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta
    29881 cgatttggcg catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt
    29941 tttggttgat accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac
    30001 ctgaccatac agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt
    30061 ccccttctgc tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt
    30121 tgatcattgc ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac
    30181 gacttgtttt tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa
    30241 agtatagtct tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt
    30301 tttgatggaa acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat
    30361 atcccctgat aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc
    30421 aacgatataa taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa
    30481 agattttctt cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg
    30541 aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac
    30601 gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt
    30661 tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac cggattgtag
    30721 ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct
    30781 aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct
    30841 aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta
    30901 tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt
    30961 gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt
    31021 atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat
    31081 aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt
    31141 ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct
    31201 ccattaataa tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt
    31261 gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga
    31321 aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca
    31381 ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa
    31441 aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct
    31501 tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt
    31561 ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact
    31621 atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg
    31681 aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca
    31741 attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg
    31801 actgctccct cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa
    31861 atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc
    31921 cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc
    31981 tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa
    32041 cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca
    32101 tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc
    32161 agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt
    32221 caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt
    32281 tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc
    32341 acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc
    32401 atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat
    32461 cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact
    32521 gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta
    32581 ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc
    32641 aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg
    32701 ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt
    32761 atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag
    32821 acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg
    32881 aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac
    32941 tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg
    33001 ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt
    33061 cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat
    33121 ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt
    33181 atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa
    33241 tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg
    33301 ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat
    33361 ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat
    33421 ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga
    33481 gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca
    33541 agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact
    33601 ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt
    33661 tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta
    33721 tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat
    33781 gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga
    33841 acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac
    33901 tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa
    33961 tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg
    34021 aatgaattag ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa
    34081 atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc
    34141 atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact
    34201 catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat
    34261 gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat
    34321 gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta
    34381 aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt caaattctat
    34441 gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat
    34501 tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact
    34561 caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat
    34621 gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca
    34681 aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat
    34741 tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg
    34801 attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta
    34861 gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg
    34921 cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt
    34981 tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa
    35041 agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac
    35101 cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg
    35161 tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa
    35221 tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc
    35281 cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg
    35341 tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa
    35401 agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt
    35461 tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca
    35521 ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca
    35581 gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag
    35641 ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat
    35701 ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc
    35761 caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg
    35821 caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag
    35881 ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc
    35941 ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa
    36001 gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta
    36061 gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat
    36121 tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta
    36181 gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga
    36241 tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa
    36301 tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat
    36361 ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt
    36421 tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata
    36481 gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat
    36541 acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca
    36601 agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact
    36661 gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt
    36721 tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc
    36781 caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg
    36841 atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac
    36901 ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat
    36961 aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt
    37021 attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg
    37081 atccgataat tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc
    37141 cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc
    37201 tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg
    37261 cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc
    37321 caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag
    37381 aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc
    37441 tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat
    37501 caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc
    37561 ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc
    37621 ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc
    37681 gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga
    37741 ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg
    37801 aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca
    37861 ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca
    37921 aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac
    37981 ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc
    38041 catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat
    38101 tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac
    38161 tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc
    38221 aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga
    38281 ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa
    38341 ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc
    38401 ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc
    38461 tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc
    38521 ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa
    38581 aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc
    38641 tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt
    38701 cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac
    38761 ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa
    38821 aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat
    38881 catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt
    38941 cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg
    39001 aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt
    39061 cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt
    39121 taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg
    39181 ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa
    39241 caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt
    39301 ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg
    39361 ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa
    39421 attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt
    39481 tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct
    39541 tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt
    39601 ggacgttctt tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa
    39661 aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag
    39721 ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt
    39781 ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg
    39841 aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact
    39901 ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga
    39961 cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt
    40021 ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat
    40081 tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc
    40141 tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt
    40201 tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta
    40261 ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt
    40321 tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt
    40381 tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa
    40441 atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat
    40501 catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat
    40561 tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc
    40621 aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag
    40681 cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt
    40741 caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc
    40801 aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca
    40861 tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc
    40921 aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa
    40981 gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag
    41041 cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat
    41101 gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga
    41161 aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa
    41221 agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt
    41281 gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat
    41341 gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata
    41401 agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc
    41461 tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg
    41521 aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg
    41581 aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat
    41641 actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca
    41701 agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc
    41761 cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact
    41821 tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc
    41881 ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt
    41941 gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc
    42001 agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa
    42061 ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta
    42121 gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc
    42181 atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa
    42241 tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct
    42301 agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc
    42361 tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat
    42421 ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt
    42481 gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac
    42541 atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc
    42601 aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg
    42661 gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata
    42721 ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc
    42781 tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt
    42841 gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac
    42901 gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt
    42961 ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc
    43021 tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga
    43081 aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata
    43141 atttactggg tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga
    43201 atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt
    43261 gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg
    43321 aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt
    43381 agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa
    43441 aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa
    43501 attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc
    43561 ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt
    43621 ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct
    43681 tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc
    43741 agtaccgatt tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag
    43801 atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat
    43861 ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc
    43921 ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac
    43981 atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac
    44041 tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt
    44101 gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat
    44161 ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag
    44221 gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag
    44281 taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat
    44341 tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata
    44401 ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta
    44461 ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa
    44521 gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc
    44581 tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg
    44641 gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc
    44701 tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct
    44761 ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt
    44821 gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt
    44881 gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc
    44941 aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct
    45001 tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct
    45061 tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc
    45121 gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat
    45181 accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata
    45241 gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc
    45301 ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt
    45361 tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta
    45421 gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt
    45481 gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa
    45541 acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg
    45601 tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc
    45661 tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac
    45721 aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt
    45781 ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact agatagattc
    45841 ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta
    45901 aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca
    45961 cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc
    46021 cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa
    46081 aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc
    46141 tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa
    46201 acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt
    46261 ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag
    46321 tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat
    46381 caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat
    46441 ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc
    46501 cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca
    46561 actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag
    46621 aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg
    46681 tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg
    46741 ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag
    46801 cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg
    46861 tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta
    46921 caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta
    46981 gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac
    47041 gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc
    47101 aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat
    47161 cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag
    47221 ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag
    47281 ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga
    47341 ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct
    47401 tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac
    47461 tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt
    47521 gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat
    47581 ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt
    47641 gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt
    47701 ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg
    47761 ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga
    47821 ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt
    47881 aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac
    47941 catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg
    48001 catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt
    48061 ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat
    48121 atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg
    48181 ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt
    48241 tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta
    48301 catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat
    48361 actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg
    48421 tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt
    48481 tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat
    48541 aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca
    48601 cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg
    48661 atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt
    48721 ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt
    48781 ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga
    48841 tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt
    48901 ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg
    48961 tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt
    49021 cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg
    49081 gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg
    49141 tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt
    49201 attaaaataa acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa
    49261 gggcgctaaa gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt
    49321 ccttcgatta atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca
    49381 ggttgttgga atgaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg
    49441 acaagtggat gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg
    49501 taaggtatat cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac
    49561 aaaaattggg gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg
    49621 aaatgtcgca aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta
    49681 aaacgattga gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt
    49741 tgaaacaatg caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt
    49801 ttcagttaac tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt
    49861 taagttacta cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa
    49921 ttatggtcaa gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat
    49981 ctttctttat tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg
    50041 aatggaaagc tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa
    50101 tcttgaaaaa tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt
    50161 ttgtcaatac gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga
    50221 tacgaaaaaa tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag
    50281 aatatgttgt tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata
    50341 cgactcattt gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac
    50401 gtctatatgc tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc
    50461 tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc
    50521 attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat
    50581 atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg
    50641 ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga
    50701 atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg
    50761 caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta
    50821 taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt
    50881 ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa
    50941 ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa
    51001 ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat
    51061 ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt
    51121 cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac
    51181 cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt
    51241 aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc
    51301 aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct
    51361 gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt
    51421 ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct
    51481 ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt
    51541 ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat
    51601 acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag
    51661 taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa
    51721 gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc
    51781 agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga
    51841 atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc
    51901 ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa
    51961 gcgtaaatct gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat
    52021 ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga
    52081 accagaagga ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt
    52141 caaagctgag acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa
    52201 tatttttttc atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta
    52261 cgtatcgaaa acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac
    52321 cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa
    52381 gcaaacaaaa gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa
    52441 gatttagcga tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc
    52501 ttctgaagtg tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa
    52561 aagtatatca tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg
    52621 aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct
    52681 gaatccttta ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt
    52741 ccatacttca aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa
    52801 taagtttcag gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc
    52861 gtatctccaa ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt
    52921 gttttaaatt catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg
    52981 aaaactgtaa cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt
    53041 gctttctctc ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat
    53101 acttgatttg catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt
    53161 ttaaactctt ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc
    53221 aagaacataa taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata
    53281 gctagaaaca tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt
    53341 gaatagtgta actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa
    53401 ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct
    53461 tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa
    53521 actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc
    53581 aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg
    53641 agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt
    53701 agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag
    53761 tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt
    53821 ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt
    53881 tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt
    53941 gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt
    54001 acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga
    54061 ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg
    54121 aagtactgga ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag
    54181 ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa
    54241 atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa
    54301 accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa
    54361 cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa
    54421 cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta
    54481 acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg
    54541 ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa
    54601 atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa
    54661 aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa
    54721 tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga
    54781 ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt
    54841 cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa
    54901 cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag
    54961 aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg
    55021 atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca
    55081 atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg
    55141 gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa
    55201 tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata
    55261 agatcatttt ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg
    55321 gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc
    55381 aatctcgggc gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa
    55441 tcgaacagca tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta
    55501 aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca
    55561 aaaatcttgt cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc
    55621 ggaaataagc cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc
    55681 ttacgatttt ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc
    55741 ttctcggcta acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc
    55801 aacttcttca cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat
    55861 agtaatcgtg tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg
    55921 tttgcgttcg atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt
    55981 gaccacatgt ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa
    56041 tttttcttct tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc
    56101 agtcttgctt gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg
    56161 tgtcatattc aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg
    56221 atctgttcca gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc
    56281 ttgtgtatcc attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac
    56341 aaattttcca tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc
    56401 cgtctcagaa tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg
    56461 gtcttcttca ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc
    56521 aaatcctagt ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata
    56581 cccttcgtta cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc
    56641 gatgaccgtt aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg
    56701 gtatattgtt acttataaat ttattctata ggagacgatt gttttctaca aatatttaga
    56761 ttggaatttg attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca
    56821 tattctaata aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt
    56881 ccactcgttg tgatggttta atttatccaa tccatcaata agcaaacaac ttatataaaa
    56941 aaggattaat tctcaatata attgctccta ccatagaata aatcttcatg taaagtaatg
    57001 aagtaccaac tatttctaat acttctttaa gaattatccc aacaatatcg ttagacaaac
    57061 cattattgaa taaatggaga gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa
    57121 ataaaagaaa gagcctcctg ccattagaac gaaccaaatc catttcattg ctcatgaagt
    57181 aaccacatag ctaaaaagag gataaatcaa ttaaaagatc aaaagaatga ctgcactaat
    57241 aaaataacta aaaatgtatc ttcttacttc atatctttta gctagtactt agttaaaaat
    57301 ttgcttccta agatcaagac aaactgtcta tcttcatttg atgaaatgcg gtaaaatata
    57361 gaaaatgaac actattggag ggaatttgta atggacagca aaactagaaa acaactgcaa
    57421 gaaaagttaa agcaacgtac acacttgctc ccttccgtct ttcaacagtt tttagaagaa
    57481 aatcgttctt ccctttcatt aggcagtcgc tatgaatacg caaaagattt tcatctgttt
    57541 actgattatt tgagaaaagt attttctgaa gaagtggatg atcagctgat tatccattta
    57601 gaaaaacaag attatcagaa tttcttagct aaaatctatc gacatactcg ttcgttccaa
    57661 actacagcga accaagaaac ggagcaattg tttgaaaata gctattacgg gatttcaagg
    57721 aagcaatatt cattaaatca ttttttacga tttttgtatc aaaaaggact gattgaagaa
    57781 gagatttctc ttttgggtac aagtttaccg gaaacaacca aagcagctcc gcgatatatc
    57841 gatgcagaac ttttcaaaga ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca
    57901 agcagagaag ctagctttga agaaaagaat agaccgagga accttgcaat tactgccatt
    57961 cttctgtatt gcggattaaa gatccatgaa gtagtagaac ttaaacgtaa agatgtatct
    58021 ttgaccaagc agattcttaa tctgaataga aaagaaaaca gactgaataa agtaccaata
    58081 ccaaaagaag cgatgccatt tctggagaac tattttaagt taacagcaca aaatacggac
    58141 gaaaattctt ttgtttttcg ttcctcacat gatcagcaaa tttctcctcg gactattcgc
    58201 caaattttga gcaaaattac agtatataaa aacgtatcgc cagaactatg cagaaaaaca
    58261 tatcgttatt atgcagaact atatttagat gatgctgacg cggtaaatta tttatgtggt
    58321 aatcgatacc tctcatccca ttcttttcaa gatatctggg aaaagatggt tgatttttct
    58381 tatcacgaat tagcggcatt cgttcgggtt tcttccatat aaaacaaatg tatgatcttt
    58441 ttttcacaat aaactatgtt attatataaa ggattatgtt acgattatct tgaatttgta
    58501 tattacttaa caaaagataa tggagtgtga atgatggaga ccataacgta ttcccctttg
    58561 aacttattta cgaattttca agaagctgca gctaactttt ccgagacccc gattatcttt
    58621 gatgagccac tttcagcttt cccagaatta ggacttgaaa caacttacaa aaaatgctgc
    58681 gaaagtgtgt tgaaacgggc ttatcaattg gctcatcttg gagtgaaatc cggagacaaa
    58741 gttattattt ataagagttc cgcttttgac acataccttc tagctgtttc tgtgtcttac
    58801 cttggtgcag tgcctgtcat gacttcttat catctaccaa caacaacaat ggaagtattt
    58861 atcgatcgtt tagaagaccc tttcatttta tttgatgatg agacaaaaaa gcgagtgaga
    58921 gaaatttcaa atggaacaaa atcaaaacag attcctattc tttatttaat ggaacaacct
    58981 gctttgtctg tttctcaatc atttttagac aaaaatgaaa tttcttatat gacccatact
    59041 tctggtacaa ctggtatccc taaacttatt tgtcattctg ctcattctat gggatggcgg
    59101 acgaaatggc aaaaaacagt tttcacaaaa atttcggaaa agaaattaat cgcttttcac
    59161 atctctccag tgcattccag attcaatatt ggtatctctt ctttgatgtc catgggattt
    59221 cctatgatgc ctttagcaaa cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac
    59281 cggccaattg cactagagac ccatccgaat aactttgttc agtggcgttt tactgctaag
    59341 gagcatccag aagctttttc gggtatccga tactatcact ccacttttga tgctataaac
    59401 aaccagacaa tgaagacatt tttgcgaacg tcgatggcac aggatgcggt atttcttcaa
    59461 gtatatggcc aaagtgaatg cggaccaatg attcttaagg ctcatacact agagagtttg
    59521 aaaacaagtg atgcaagaga tatgggcgtt ggactggaag acatgacaag cgctcggatt
    59581 acagattcag ttgggaatgt cctacctgaa aacacagatg gtcatataca attactgtct
    59641 aaaggacggg ctttgactta ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc
    59701 gattggtggg atagcggaga ttacggtttt atggataagc atggccacct atttttgaaa
    59761 gatcggcaag tggatttgat cgaaacgatc aatagtacat tagctattga agacttttta
    59821 ttagattcgt tagatttttt ggcagaagta gtgattgtaa gggataaaaa tcattctcca
    59881 caaccgatta ttgctttagc tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa
    59941 gtccatgaac ttcctcgctt gaacgtccct atcatccgtg attttgatgc aatcccgcga
    60001 acagctacaa tgaaagtgca acggcttcaa atcgagaaag aattaaaatc acaaaagtcc
    60061 taactattta aggaaagaaa gatccatttt ggtctttctt ttctttttgt ttttttaaat
    60121 tggatgagat aaaaatatgc caattggctg tttttctttt ttgaaaattt cgttatactc
    60181 ttattcatgg aggaaaatgt tatgaaaaga atcttgacaa ttggcggttc tgatccattt
    60241 gctggtggag gaatccaaac agatttaaaa acttttgaga attttggtct attcggacta
    60301 agcgccttga cttctatagg ggcactagat attaatgagc gatttatgct ggaatctata
    60361 tctttggaat tgcttaaacg acagttagtt tcgattgata aaatgacttc attagatggt
    60421 atcaaaattg ggttattgaa ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa
    60481 acaaaacaaa atatcccaat cattttagac cctgttttgg cattcaaaga aactgaagct
    60541 gtgatcaatc aattgtatat ttcgcagctc atcgaagaac tttttccact tgttacgatg
    60601 attacgccta atttggcaga agctgcttta ctttctggtc aagctcttcc ctcttcgtta
    60661 acagaaatga tccatctcgc taaagaactc actgcttcag gaacaaaaaa cattgtgatc
    60721 aaaggcggca caaggatggc tggagatgaa gctgtggatc ttctctattc aattgatcat
    60781 tatgaggtat tttctcataa aaaaatcgac actacaacag taaacggggc cggttgtgcc
    60841 ttttcttctg caataacagc gaatcttgtg ttaggggcag attttaccaa agcagtagca
    60901 gaaagtaaaa aatttgttgc ccattgtatt tctaatggcg tgctgatgaa cgatcatacc
    60961 ggaagtgtct ggtctggcgg tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt
    61021 aacgatctat gctatgctga ttgcgttgac agttgccctt tcattaacaa ttttgatccc
    61081 tgtaccggct acgaatggat ttgtgacatt atgcgaggcc gggatctata ccgctgcttt
    61141 tctttatggt ccaacgggtg gattagttgt tggtgcttcc agtggtctat tgatcgattt
    61201 gatttcagga tatccgcagt gggcagtttt ttcattagtg atccacgggt tacaaggata
    61261 tgttgctggt tctttcagca aatcttctac tcgtatgtgg ggattagggc ttttattagg
    61321 aagcttagtt atgatcatcg gctatggtat cgccggttgg attttatatg attggccggc
    61381 tggagtggct tctataccag gaaacatctt ccaaaatctg attggaatgg gacttgcctt
    61441 tcctataact gcaagtcttc aacgtattca agcacggaag caatattaat tgaaaaagaa
    61501 ggttttagaa aatgaagttg tcagaaaaag aattaaaaga ccaattgact gaaatagtta
    61561 atgacatatt ggctgaggca cacttgaaaa aaggtgatct ttttgtttta ggatgcagca
    61621 caagcgaagt cgttggagga catattggaa aaaattccag tgcggaagtt ggtcaatgga
    61681 tcatccgtac gttaaaagag ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt
    61741 gtgaacattt gaaccgagca ttagtagtag aacgaacagt ggcagaagca aaaaatttcg
    61801 aaattgtctc tgttgttcct gcactacatg ctggaggtgc ttgttctgtt gctgcttttg
    61861 accagttcaa tgatcctgtc gaagtggaac acgtagttgg acaagccggt atcgatatcg
    61921 gtgacacatc aatcggtatg catatcaagc atgtccaagt acctgtcaga cctcgtttga
    61981 aaacccttgg acaagctcac gtgacagccc ttcgctctcg tcctaaatat atcggtggtc
    62041 ctagagcgaa ttattgaaat gagtaattga cagagcaatc tgaaaaactt cctcgcaaag
    62101 tccaactaat gaactttaac gaggaagttt tacttgtttg ttttatataa ccgagcttca
    62161 taaggtgcta gtatgccttt agcttcaaat tctccgctag aggaatttga caagagtaat
    62221 gtccaggact gatctgcttc ttttggaagt tcatagtcag caagtgattc tgttaagttt
    62281 accataataa gatattgttc atcttctaaa tatctttcat agacataaag ttgtggatgt
    62341 tcatgaagat attctttata agaaccgtat atcagacctt tattggtttg tctaatgcgg
    62401 atcatgttct tatagaattg caggaccgac tctggttcat attcttgttg ttctacattg
    62461 atcgttttgg tatttggatt agtgatcagc caaggttcat gtgtagaaaa accagcatat
    62521 tgttccgaag tccattgcat gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata
    62581 tccaatgctt cttctcttgt cttaccttcc gcaagcagcc ttttataaag tcttttcgaa
    62641 tctactgcat caacttgatc gatcgattca aagggcatat tcgtcattcc gatttcctgt
    62701 ccttgataaa taaatggcgt accttgcaaa aagaaataca tcatagcgat tgcttttgca
    62761 gacatagccc aaaaatcggg acgagtatct ccaaacactg aaacggcacg aggaacatca
    62821 tggttttcca tatataaagc gttccatccg ataccatcca atgaagtttg ccaagcgctt
    62881 aatgcatgtt tcaatttaag gacatctaat tgcccttctt tttcttgctg ccagatatgg
    62941 atatgatcaa attcgaatat catatcgaaa taaccgtctt tccctaccca ttgtggtcct
    63001 tcttctgcag tgacaccact tgcttctccg acagtcatga tatcgtactc ttcaaaaaca
    63061 tgtttcaaat cggtcaaatg ttcttcgatt cctgagacat tttgaaaggg tgagaaaggg
    63121 ttctctgtgg cttttactga atattcatct ttttttacat gagaaatcgc atctaaacga
    63181 aatccatcta tccctttatc caaccaccat cggatcatat tgaatagttc ttctttcaat
    63241 ttctcgcttt cccaatttaa gtctggttgt tcttttgcaa aaacatgcag ataatactgt
    63301 ccagtttcat gagaatactc ccaagcagaa ccgccgaaaa tcgatgtcca attattcggt
    63361 tctttgccat ctaccccgtc gacccaaatg taataatcgc gatacgggct ttctttagat
    63421 tttttcgatt cttcaaacca ttgatgctga tccgaactat ggttgatgac aagatccata
    63481 atcactttga tccctaattc atgtgcttct tttagcaata aatcaaaatc ttccatcgtt
    63541 ccgaatctct cttctatatc ttgatagtca cttacgtcat aaccattatc gacaaatgga
    63601 gattgataga tcggattgat ccagataaag ccgataccaa gatctttcaa ataattaagt
    63661 ttttcacgga tcccattgat gtctccttga ccatcgccat tgctatcttt gaaactagca
    63721 ggatagattt gatagcctac tgcttctttc caccattttt gttcttgtat catatcgttt
    63781 cactccttta acggtccctc ttgattataa ccgattctat tttagaagta tccttgttac
    63841 cggtaactag aaacgaaaag ctcaacgaat ctgctaagtg cgttcgctga gctttatttt
    63901 atcatagata gcgggcaatc agttgaatca tttgcttggg aaattcattc aagtcagtaa
    63961 tatctacaaa acgactttca ccgtagatcg acttgatttc ttctttgtct tggccaatag
    64021 ctgcggagac aaataggatt ccttgacgtt catattcttt caaaacagac tggatatctt
    64081 cttttgcttt tttgcctgta taatccggca acgcttttgg ctgaccgtca ctaatattaa
    64141 gcaacagttt ggttgttgcg gattgtctgt ttaatttttc tgctgctaaa cgaagtgcag
    64201 caccatcacg attgttttgc cttggtttca tagtgactaa tttttcattt aaccattgaa
    64261 atccgtcttc aaattcttta taagaaaaca aagaagtctt ttctcttgac gaacggtctg
    64321 ctgtatctcc gtaaatcaac aatgggatat cgactttctt agaaaattcg gcaatggcaa
    64381 tcgctgcttt ttttgccgct tcgatccgat catcccgtat cattgaccca gattcatcga
    64441 tccgtacagc taccgctaaa gaaggttgct catgtggcgg gttctttttg tcaaaatttc
    64501 tcaaatcacc atatgctact ttgcttgcat tgaaccgctg accttcgtat tttccttttt
    64561 ggtacgtctc tgtttgttca ttatccagta actctaaaat ttgccggcta agtgattcta
    64621 ctattggcat tacttgcatg tgtagctcac gtgcttccgg cgtttttcct tggaaagctg
    64681 gtcggtgaac gatgagacct tctttttcat gaatcgtttc cttcatgatt tgtcgcgctt
    64741 ctttattcag ttgcttcttg atttccttat catgtgcttc catgtcagaa gcagacattt
    64801 cttgagaatc atctcctgtt tgttgttctt tttgattttt tccttcgata tcgctactgc
    64861 gttctttttg catttgagga ggaatctcgt ttttttctat tgtttcttct tgtttctctt
    64921 ctccctcatt tggttcttca gcagacaacg atttcttttc atctaagcgc agaatttctt
    64981 cagcattttc agtgatgttt cgttcatttg cttcttcggc gctaatggtt ctttcttttg
    65041 tatgctcaga ttgaggatga ccccattttt cttttctcaa tatgtcctga agttccagta
    65101 gcaatccact gtcagacaaa gcttgttcaa gaattgccaa ttcatctgaa tcagtggata
    65161 atttatacaa aacttttgga taaatcgact ggagtaaatc ttgtccttgc ttataggtat
    65221 ttgcccaata aaaataagaa cgcattcctg ctacaccttt gatggcgtta gcttttgcag
    65281 tttcatccaa caaacggata atctctgcca tctttaagag aagttcttgt ttttggatca
    65341 tagttttcgc catcgcacgt tctgccatga cttctaaggc aggaagatcc attttttccg
    65401 catgttgcat ccgatctctt aaagattcat ttaatgggcg atttccttgg taatttcgat
    65461 ttgtagtgat cacaatgacg caatctggat ggcgacgaat gattcctgtc ggtaaattca
    65521 acatcccatt tggttcgagc gctgagttaa gtgccacaag aacagaggca tctcggatga
    65581 cagtcggctc ttgaatctct aataaatagc ctttttcaat cgctcgaacg atttcagaag
    65641 ggtaatagct gtaatggatt gcatctggat aatcagcttc gatcctttga actagctctg
    65701 ctaatttttc tttagcagaa aaaggatcaa tgccgtaatg gttctggatc agtgtcagta
    65761 cagcttcaag tgtatctgtt tggtaaatag cttctagtaa ttcttggtct tgttccttat
    65821 tagaatctac tacaggtaat aaggctccga aaacatctga tttgtccata tctgcaaaac
    65881 aggtcacttt tgtataggga agttgcagat cagcagatag tgctttggct aattgtgtct
    65941 tccccgaccc ggcatctcct tccaaaagta cgttactgat tttcatttca ggatcttgcc
    66001 aattcccttt gatttcggag gcgatccgcc attcttctgc acttgtccga tggcttttcg
    66061 gtttcttcca gatcatttgc ttttctagcc gtgaaaaggt tcggttttct gttagtgaga
    66121 aattctccat gcttctcttc cccttttgat catctctgtc tattaatatc ctagctcatc
    66181 taaaagacgt tttaaaatac ggaatcgttc cccaatcagt tctccgtctt tttgtagtgt
    66241 atcgtaatac aaaatgatgt cttcatcgat ctttggattt ttggtatcta cttctaccgt
    66301 cattcggttc ccttgtaatc caatgatgtc tacgactgga ttttctggat cataaaattt
    66361 ttcataacga taagcatctt ggaagtataa acttccttgc gcagctaaga tcgctaaatc
    66421 taaaatacgt gtgattggta attcttccga ctgtcgtgac catttttctc cagtatgtcg
    66481 ccataccttg cctgagatat cgacttttcc acgatcattc cactgagcaa ggcccagtga
    66541 aagccctttt gcatccatat ttaatgcatt tcttccatca actttatcat agtcttctac
    66601 aacgattact ggtttatgtt ttaaatttgt tggtatttcc attattttaa ctcctcatcc
    66661 ttttattttt agtaaataag taaattagta gtttactcat ttactaaact atatgcgaca
    66721 ttatagagga ttgaagtgag gattgcaaag aaaaagagct tcattgatag aaataaaaac
    66781 accatctcgt tcttttacac gctcattagt aacatgtaga aagtccaaga tgatgttgga
    66841 at
//
LOCUS       DACEDO010000007        57556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEDO010000007 DACEDO010000000
VERSION     DACEDO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.007"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..816
                     /locus_tag="IWO52_000486"
     CDS             4..816
                     /locus_tag="IWO52_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0354778.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IWO52_000487"
     CDS             813..1613
                     /locus_tag="IWO52_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0354779.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IWO52_000488"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IWO52_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0354780.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IWO52_000489"
     CDS             3110..4819
                     /locus_tag="IWO52_000489"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0354781.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IWO52_000490"
     CDS             5031..9383
                     /locus_tag="IWO52_000490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0354782.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IWO52_000491"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IWO52_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0354783.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IWO52_000492"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IWO52_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0354784.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IWO52_000493"
     CDS             11221..11514
                     /locus_tag="IWO52_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0354785.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IWO52_000494"
     CDS             11511..11822
                     /locus_tag="IWO52_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0354786.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IWO52_000495"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IWO52_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0354787.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IWO52_000496"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IWO52_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0354788.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IWO52_000497"
     CDS             complement(14623..14844)
                     /locus_tag="IWO52_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0354789.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="IWO52_000498"
     CDS             15292..17655
                     /locus_tag="IWO52_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ0354790.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAKKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="IWO52_000499"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="IWO52_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0354791.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="IWO52_000500"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="IWO52_000500"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0354792.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20243..21028
                     /locus_tag="IWO52_000501"
     CDS             20243..21028
                     /locus_tag="IWO52_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0354793.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21041..21892
                     /locus_tag="IWO52_000502"
     CDS             21041..21892
                     /locus_tag="IWO52_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0354794.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22016..22468)
                     /locus_tag="IWO52_000503"
     CDS             complement(22016..22468)
                     /locus_tag="IWO52_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0354795.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22933..23301)
                     /locus_tag="IWO52_000504"
     CDS             complement(22933..23301)
                     /locus_tag="IWO52_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0354796.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23451..23933
                     /locus_tag="IWO52_000505"
     CDS             23451..23933
                     /locus_tag="IWO52_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0354797.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23970..24980
                     /locus_tag="IWO52_000506"
     CDS             23970..24980
                     /locus_tag="IWO52_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0354798.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25095..25808)
                     /locus_tag="IWO52_000507"
     CDS             complement(25095..25808)
                     /locus_tag="IWO52_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354799.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25998..26588)
                     /locus_tag="IWO52_000508"
     CDS             complement(25998..26588)
                     /locus_tag="IWO52_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0354800.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27072..27299
                     /locus_tag="IWO52_000509"
     CDS             27072..27299
                     /locus_tag="IWO52_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0354801.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27315..28481
                     /locus_tag="IWO52_000510"
     CDS             27315..28481
                     /locus_tag="IWO52_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0354802.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28715..29758
                     /gene="hrcA"
                     /locus_tag="IWO52_000511"
     CDS             28715..29758
                     /gene="hrcA"
                     /locus_tag="IWO52_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0354803.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29852..30415
                     /gene="grpE"
                     /locus_tag="IWO52_000512"
     CDS             29852..30415
                     /gene="grpE"
                     /locus_tag="IWO52_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0354804.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30466..32295
                     /gene="dnaK"
                     /locus_tag="IWO52_000513"
     CDS             30466..32295
                     /gene="dnaK"
                     /locus_tag="IWO52_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0354805.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32446..33612
                     /gene="dnaJ"
                     /locus_tag="IWO52_000514"
     CDS             32446..33612
                     /gene="dnaJ"
                     /locus_tag="IWO52_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0354806.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33694..34359)
                     /locus_tag="IWO52_000515"
     CDS             complement(33694..34359)
                     /locus_tag="IWO52_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0354807.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34335..34508
                     /locus_tag="IWO52_000516"
     CDS             34335..34508
                     /locus_tag="IWO52_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0354808.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34736..34942)
                     /locus_tag="IWO52_000517"
     CDS             complement(34736..34942)
                     /locus_tag="IWO52_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0354809.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(34968..35387)
                     /locus_tag="IWO52_000518"
     CDS             complement(34968..35387)
                     /locus_tag="IWO52_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0354810.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            35891..36244
                     /locus_tag="IWO52_000519"
     CDS             35891..36244
                     /locus_tag="IWO52_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0354811.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(36304..36471)
                     /locus_tag="IWO52_000520"
     CDS             complement(36304..36471)
                     /locus_tag="IWO52_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0354812.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(36590..38509)
                     /gene="tet(M)"
                     /locus_tag="IWO52_000521"
     CDS             complement(36590..38509)
                     /gene="tet(M)"
                     /locus_tag="IWO52_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0354813.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(38525..38641)
                     /locus_tag="IWO52_000522"
     CDS             complement(38525..38641)
                     /locus_tag="IWO52_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0354814.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(38886..38963)
                     /locus_tag="IWO52_000523"
                     /pseudo
     CDS             complement(38886..38963)
                     /locus_tag="IWO52_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(39117..39752)
                     /locus_tag="IWO52_000524"
     CDS             complement(39117..39752)
                     /locus_tag="IWO52_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0354815.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            40264..41406
                     /locus_tag="IWO52_000525"
     CDS             40264..41406
                     /locus_tag="IWO52_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0354816.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            41399..41605
                     /locus_tag="IWO52_000526"
     CDS             41399..41605
                     /locus_tag="IWO52_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0354817.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            41684..42877
                     /locus_tag="IWO52_000527"
     CDS             41684..42877
                     /locus_tag="IWO52_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0354818.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(42884..43042)
                     /locus_tag="IWO52_000528"
                     /pseudo
     CDS             complement(42884..43042)
                     /locus_tag="IWO52_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(43048..43785)
                     /locus_tag="IWO52_000529"
     CDS             complement(43048..43785)
                     /locus_tag="IWO52_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354819.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(43787..45289)
                     /locus_tag="IWO52_000530"
     CDS             complement(43787..45289)
                     /locus_tag="IWO52_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0354820.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            45548..47542
                     /gene="uvrB"
                     /locus_tag="IWO52_000531"
     CDS             45548..47542
                     /gene="uvrB"
                     /locus_tag="IWO52_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0354821.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            47552..50368
                     /gene="uvrA"
                     /locus_tag="IWO52_000532"
     CDS             47552..50368
                     /gene="uvrA"
                     /locus_tag="IWO52_000532"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0354822.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            50631..51896
                     /locus_tag="IWO52_000533"
     CDS             50631..51896
                     /locus_tag="IWO52_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0354823.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            51900..52631
                     /locus_tag="IWO52_000534"
     CDS             51900..52631
                     /locus_tag="IWO52_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0354824.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            52766..53650
                     /gene="rapZ"
                     /locus_tag="IWO52_000535"
     CDS             52766..53650
                     /gene="rapZ"
                     /locus_tag="IWO52_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0354825.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            53647..54645
                     /locus_tag="IWO52_000536"
     CDS             53647..54645
                     /locus_tag="IWO52_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0354826.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            54679..55614
                     /gene="whiA"
                     /locus_tag="IWO52_000537"
     CDS             54679..55614
                     /gene="whiA"
                     /locus_tag="IWO52_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0354827.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            56157..56807
                     /locus_tag="IWO52_000538"
     CDS             56157..56807
                     /locus_tag="IWO52_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354828.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            56994..>57556
                     /locus_tag="IWO52_000539"
     CDS             56994..>57556
                     /locus_tag="IWO52_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0354829.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        19160 a        10055 c        12049 g        16292 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac
    18181 aaaagttatc gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct
    19021 gggatttttc gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa
    19621 agaaggtgtt tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa
    20041 ataagccgaa atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata
    20101 aaaggacact agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata
    20161 tatcttggtg tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag
    20221 gtatgaaaaa ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac
    20281 tggtggctca aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa
    20341 tgtagttgtc aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga
    20401 agaggcaggc ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca
    20461 gcgtttactt gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc
    20521 tgggatggaa aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt
    20581 aaaagtcaat ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga
    20641 gaataataaa aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc
    20701 aacgtttgct cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc
    20761 aatggagtat gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac
    20821 acctattaat gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat
    20881 cccactagga cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc
    20941 agacgaatcc agttatgtta caggtattac actatttgca gatggtggga tgactttata
    21001 tccttctttc caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt
    21061 gatcccggca gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc
    21121 aacaaatgag atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt
    21181 attcgcttct ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc
    21241 attttgggtt attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa
    21301 aacgatgcca atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc
    21361 ttttggagaa tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct
    21421 tgtatttggt tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac
    21481 aaaaggaatt agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc
    21541 taaactagtt gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt
    21601 aggggcagtg atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg
    21661 gaaagcaacg atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc
    21721 aaatgaaaat ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac
    21781 attaggcggt atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat
    21841 tatcggatta ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag
    21901 atggtaaaat ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa
    21961 aaaaatagcg ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag
    22021 atacaaggga aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta
    22081 cgacataatt gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt
    22141 attgtaaaag acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt
    22201 ctcggaattg attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg
    22261 gtgcatttag catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata
    22321 ggaactcaaa aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta
    22381 gttttccttc atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta
    22441 attcatttac tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga
    22501 cttagttgat atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg
    22561 taaaaatcaa gtcagaaaat tctaactttt atttctctaa agaaattcgt tctctcctaa
    22621 agtgatatta tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat
    22681 attttctcgt attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt
    22741 ttactaagta gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact
    22801 ccttataaaa atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga
    22861 taggattgaa caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat
    22921 tcgtataaag ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga
    22981 aatcaattgt tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt
    23041 atccgtatat acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt
    23101 ttccggtgcg atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg
    23161 gtcagtaaac ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt
    23221 tatcttagca aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat
    23281 gatcggctga aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc
    23341 agaaaaacaa tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat
    23401 atttatatga taagatgtct caaataataa aatagaaaag agacataaaa atgattgact
    23461 tggcaaagaa attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt
    23521 atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca
    23581 aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga
    23641 aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat
    23701 cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg
    23761 aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg
    23821 caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc
    23881 agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta
    23941 aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg
    24001 caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg
    24061 aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt
    24121 ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa
    24181 tgatgcaaga tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg
    24241 agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag
    24301 atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa
    24361 ctaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg
    24421 tactgaaaac aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag
    24481 tgtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc
    24541 aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata
    24601 cgatcaggca tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata
    24661 atgatggaaa gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa
    24721 gaagatcact tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt
    24781 tcggtagaag tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc
    24841 tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac
    24901 aactgacatt gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg
    24961 ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa
    25021 atctaaatag aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata
    25081 acatattaga aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg
    25141 ggatttcctg taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct
    25201 ctaatgaacg ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga
    25261 taactagtga ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat
    25321 ttcccagtga gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg
    25381 ataggtgttc ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta
    25441 aaagtattac ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat
    25501 cctgctaacg gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa
    25561 gaaatatttg tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta
    25621 ctgcttgttg aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca
    25681 gctgaactgg tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct
    25741 ttactaggag acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac
    25801 tttttcatat aaaatacctc attatttttt taataccagt atagcataat cctgaatttt
    25861 tgaatccgta aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca
    25921 gaaaaagtgt gacaaataat catcagtaaa attacttgtc acactattga taaggagtat
    25981 ttaattgaag agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat
    26041 ccaagtttgt atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct
    26101 tgaagaatca agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga
    26161 tctttcacag cttgttgttc tataaaatct tctttattct gataattttc cgattctaac
    26221 caaggaaatt gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa
    26281 taatcaactg ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt
    26341 atttgatata aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg
    26401 tcaacttttt caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg
    26461 gaagtgacct ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt
    26521 gtgctagtaa tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca
    26581 gttttcattt tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag
    26641 acattcatta aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt
    26701 atacagatag tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg
    26761 tttaccagca ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct
    26821 tgagtacaat attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat
    26881 catatcgggc actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta
    26941 atgaaagaaa aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca
    27001 aatacgttta gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta
    27061 aggaggaatc tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg
    27121 tcagctttgc gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca
    27181 ataagcaagc atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg
    27241 agttggtgct tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag
    27301 gagaaagaat ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca
    27361 ttcatatccc attttgcgag catatctgct attactgtga ttttaataaa gtttttttag
    27421 aagggcagcc agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag
    27481 aaagtcatcc gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt
    27541 tatctgctaa acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa
    27601 atggtaatga gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag
    27661 taatgaaaaa ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct
    27721 tattgaaaaa aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt
    27781 tagaagcaga aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa
    27841 cactagaagg atatctggat actttggaaa aagctttggc tttggatctg cctcactatt
    27901 ctttgtactc attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa
    27961 gactcgaact gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa
    28021 tggaaaagca tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat
    28081 ctcaacataa cctcatgtac tggaacaatg accactattt tgggtttgga gctggtgcta
    28141 gcggttatct tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc
    28201 cattacgagc aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa
    28261 tagaagaaga aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct
    28321 atgatcgcta tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc
    28381 aagaagggtt acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt
    28441 tggggaacaa tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt
    28501 ttatctgaat ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat
    28561 ataactggat ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa
    28621 tggagtcttt ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact
    28681 tagaatatat gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat
    28741 tttacgtctg atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt
    28801 gatgtctgag ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga
    28861 agatgaagga ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg
    28921 ctatcgttat tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct
    28981 gctgattcgt cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc
    29041 tgctgagata ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa
    29101 agaccgccga ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat
    29161 cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg
    29221 aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct
    29281 gatgacggtg taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca
    29341 aacgacagaa gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa
    29401 ggtttatgtc agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca
    29461 gttcaaatcg atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc
    29521 gatggatagc aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa
    29581 tatgagtatg attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt
    29641 gttagggcct actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag
    29701 agaactagca gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg
    29761 tgaataacga agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca
    29821 gctgactgaa taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa
    29881 agaaatgaca gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg
    29941 tatttcagaa gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga
    30001 aatggaagat aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa
    30061 aaatgagcgt gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc
    30121 atcaattgac aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg
    30181 tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg
    30241 aattgaaaaa atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca
    30301 aactgttcca gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg
    30361 ctacaaatta catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg
    30421 cggatataaa acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc
    30481 ggtattgact taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa
    30541 atcatcgcta acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga
    30601 gaaatccaag taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca
    30661 tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat
    30721 acaccacaag aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat
    30781 ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa
    30841 cgtcaagcaa caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat
    30901 gaaccaactg cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc
    30961 ttagtattcg accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt
    31021 gtctttgatg tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac
    31081 aaaatcatcg actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa
    31141 gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct
    31201 ggcgtatcaa gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg
    31261 catttggaaa tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa
    31321 cgtacaaaag taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc
    31381 gacgaagtta ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt
    31441 aaagaaacag gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt
    31501 gctgctatcc aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt
    31561 acaccattgt cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc
    31621 aatacaacga ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca
    31681 gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta
    31741 ggaagattcc aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta
    31801 acattcgata ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa
    31861 aaagaacaaa aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt
    31921 atggtaaaag atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac
    31981 ctacgtaatg atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa
    32041 ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc
    32101 gctgttgaag caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc
    32161 gttcaaaact tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac
    32221 ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat
    32281 ggcgacgata aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc
    32341 tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga
    32401 taacaagcga cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt
    32461 gattattatg aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct
    32521 tatcgtaagc tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa
    32581 aaattcaaag aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca
    32641 tatgaccaat acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc
    32701 ggatttggtg gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc
    32761 gactctttct ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct
    32821 gatttgcaat atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa
    32881 atcaagtata atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc
    32941 acacaaccaa ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa
    33001 acaccacttg gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa
    33061 gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca
    33121 gttaaagtga atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa
    33181 ggagaagctg gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa
    33241 gaaagtgata tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt
    33301 gtacaagcag ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg
    33361 aagatccctg ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg
    33421 aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa
    33481 aatctaaatg aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa
    33541 gttgaagaac agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa
    33601 aagcggaagt agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa
    33661 caatcatatg ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag
    33721 ctttagaata tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt
    33781 gttcaatcga tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat
    33841 gcttccgtta tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc
    33901 tgtcaggctt ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg
    33961 taaaacgatc ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa
    34021 agctatccat tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt
    34081 ataacaaagc gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt
    34141 ctggatttta tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat
    34201 gatttgttgt ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag
    34261 caactccaat aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc
    34321 cacaactaca taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact
    34381 atgtacttga tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc
    34441 gattgcttgt ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa
    34501 gaagataaaa atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaattt
    34561 tatatctaat tttcaattat ccgcataaac actatacatt caagcatttt gaagtataga
    34621 aaaacactca tcttaccacg aatttaccac gattgaatga gccttgatat gaccgtaaaa
    34681 ctactaaggg agataacacg aaactgtgta tctcccgatt tttttataca gttgattact
    34741 tcactccgat ttcaaatttc aatattgctt tcatcatgtc tgtatgtatt tctcctttta
    34801 gttcttcatc aacctgcata atgattttac cactttcaga gtggtaaaaa ggacgcaagc
    34861 ataatttaga aatataaccg tcataaaact tcaaaatctc tttaactgcc atttcatctc
    34921 cgcttgacgc tttgcaaatc aattcaaaag agggatagcc ataatcttta cttcctttcg
    34981 ctcaaaagca tttttaactt ccttagtcct ctagttcttc tactgcaaat agcagaacgt
    35041 gatacatggt acagttctga aatttctctg tcgttcattc cttgaaaata acgtaataaa
    35101 atcgcactac gctgtttttc tgatagtcta tgtaaaactg taccaagtgt ttcatcagat
    35161 acataaattg taaagtgcaa gactttaaat gatacacaat cataaaaaca ggtatcaatc
    35221 gttccacgtt caacttgttt cgtttcggct atttcacaaa acaatacttc acgtttagaa
    35281 tgtcttgcaa gctgtctgtt tcgacttttc actttgttct taatgacaat cagcattaaa
    35341 gaattgaact gtaatctgac gatatgctcg aaagaagatg tcctcataat ctcaccccct
    35401 ttctgtctag gaaaggaagt gagccttgct cgtttatctc ctttcactct tagtcccaat
    35461 gtgaaagggg gatttgttgc attactgata aataaacttt gtaaaaaagt tctgaatagc
    35521 caaaaaagca tataaacaga tttatttctc tgtttacatg cttctgttat tctatctata
    35581 tgatttataa aaccacattg gtggacgtac ttatctattg cagatagacg actttttttg
    35641 acaagaaccc aatgtaagga aatttattgt atatgatgta cttcatggcg acgttgacct
    35701 ccaacaaacc gccatttgga agtaatatac aatattttaa cagcgtaaat agcactacca
    35761 tataacggtt ttttttattg gcgtttagta gtgcttttta ttaaatataa acctataaac
    35821 catataacac gtttttctat acctgttttt aattcagtag gaacaataaa atgtatagag
    35881 gtggtctact atgcgtaaaa aagaagataa atatgatttt agagcctttg gtttagccat
    35941 taaagaagct cgattgaaac gaggtttaac tcgtgaacaa gtgggagcat tgattgaaat
    36001 tgacccacgg tacttaacta atattgaaaa taaagggcaa caccccagca tacaagttct
    36061 ttatgacctt gtatcgttac ttcatgtttc cgttgatgaa tttttcttac ctgctaataa
    36121 cttggtaaaa agcacccgac gattacagat agagaaatac atggatagct ttacagacaa
    36181 agaactatcc ttaatggaat ctttagccag cggtatcaac gaagcaagaa acatcgaaga
    36241 ctaattaaaa gaatccatac ataacggaaa gagccgataa aatgagattg tattaatctc
    36301 attttatcgg ctctgcgtct ttgcgtctgg ctctgtaatc acagttactt tgaactgctt
    36361 tatttcaatt aaattttctt gtctgcattt cggacaatag agggggaatt tttttaattc
    36421 agtatcttcc cttatcttta atcgtgtttt atttccacat acaggacaca atatccactt
    36481 gtagtttata ataactatct cctcctttac actttaattc aaatctttat taaaaaatat
    36541 ttcatcttat ttaacaagaa accatattta tataacaaca taaaatacac taagttattt
    36601 tattgaacat atatcgtact ttatctatcc gactatttgg acgacggggc tggcaaacag
    36661 gttcaccggt agtaacatgg taccctttta actctgttaa acaaacacta cgtccatttg
    36721 taaagaaagt taaatcacta cgatattctt gaatacaccg agcagggatt tctccactaa
    36781 gaatgacctc attatttttc aattgagtgt ctacgatgtt cgcacaatat ttaggagcat
    36841 cgttgtatgc tcgtgaaaga tattcctgtg gcgcataaat tttaaaacta agatatggct
    36901 ctaacaattc tgttccagct ttttttaaga cttgttccaa tacaatagga gcaagcatcc
    36961 gaaaatctgc tggggtacta acagggctat agtataagcc atacttaaaa cagattttac
    37021 agtccgtcac attccaacca tacaatcctt gttcacagcc atagcgtatc ccctccataa
    37081 ctgcattttg aaacgattga tttaagtatc caagagaaac cgagctctca tactgcattc
    37141 cacttcccaa cggaagcggt gatacagata aaccaatgga agcccagaaa ggatttggcg
    37201 gcacttcgat gtgaatggta tattctgcat tttttaacgg tctctccata taaatgactg
    37261 taggctcttt tagttctatc tccacatgat acttttcttg caacagtgca ctaatcactt
    37321 ccatttgtac tttccctaag aaagaaagta taatttcatg tgtcgtagaa tccacgtaat
    37381 atcgtagaag cggatcacta tctgagattt ccaaaagggc atcaagcaac atttctctct
    37441 gttcaggttt actcggttca acagttgttt gtagtagagg gtgcggattt tcaatctttt
    37501 ttctctgtgg caatagtttt gtatctccaa gaacactatt taacttcaaa aactcatttt
    37561 gcaaaataac aatttctcca gaataagctc tatcaatctt acataattca ccatttattg
    37621 aagtatacat ttctgtaact tttatttttt ctttttctga tactctaacc gaatctcgta
    37681 aatgtagtac tccactataa aggcgtatat atgcaagacg ttgtcttttt tttgtatatt
    37741 caattttgaa aacatttccg caaagttcag acggacctcg atgtgttgat gaataaaatt
    37801 tattagtaat aacttctata aggttatcaa tccctatatt actttttgca cttccatgat
    37861 aaagagggaa cagagaacaa ttctgaaatc ttatgctttc ctcttgttcg agttccaatg
    37921 cttctaatga tttaccggac atatatttct ctaaaaggtc atcgtttccc tctattaccg
    37981 tatcccattg ttcagattcg gtaaagttcg tcacacacat attaggatac agttctacct
    38041 tctgtttgat tacaatttcg gcagaaagtt tctctttaat atcctgataa accgttgata
    38101 aatcaattcc attttggtca atcttattga taaaaaagat tgtgggaatc cccattttcc
    38161 taagtgcatg aaataatata cgagtttgtg cttgtacgcc atcttttgca gaaatcagta
    38221 gaattgcccc atctaaaact gataatgaac gatatacttc tgctaagaaa tccatatgtc
    38281 ctggcgtgtc tatgatgttc accttcgtat tttcccactg aaaagaggtt attcctgtct
    38341 gaattgtaat tcctctctga cgttctaaaa gcgtattatc cgtcctcgtt gtacctttgt
    38401 ccacgcttcc taattctgta atcgctccac tgttatataa taagctttct gttaaggtag
    38461 tttttcctgc atcaacatga gctaaaactc caatattaat aattttcatg tgattttcct
    38521 ccattcaaaa acccaaaagg gcataaaaat cccagtgata aatactttta tcactgggat
    38581 ttttatgcat aaccataggc atacaaagca tacagatatt ctccggatac tttagaatca
    38641 catgataaag gtattcttaa actgggtaca aaaaactaag ccctcctaaa aaaggacatc
    38701 caattatttg ttcccactat caaattgaca gtttatttaa gaataccttg ccgcatattt
    38761 attaactcct tttaaataga tacttaaata atagcacgta agagcatatt tgtaaaggaa
    38821 tctccaattt tttatcaaag agagtacgtg attacaaaat agctgtaata atgtaccaat
    38881 atttgttatt ctataatctt ccaattactc ccgttctttt caagtaccaa atcaaattga
    38941 gatacctgcg ttgcctttgc ctgatttacc acaaatcaga ttttatccct ttcttcctct
    39001 tgattgccca caacagaaaa gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc
    39061 tttgcactct tgatcgggct ttttgtttgc tgtatcttct cgcaagagga aaaaagttac
    39121 tcgtcctcat catcaaaatt gttattcctt aatacttctc gtaacagttc catatcttct
    39181 tttgagttcg ggtttatcat tttcacgact tgataaggtg tcttatactt atgtccctct
    39241 atgctttccc caaacatatc catactgtat aaatcatcat agcggtctaa taatttttga
    39301 aacttcaaat gctggataaa ttctttgatt gggtaaaggt cggacgcttc aatgcttttc
    39361 ccgttgatat agtctgtaat atattcccgt aacttttcta actcatattc cagccattct
    39421 tcctcgctgt caaagaagtt atcatagtgt ccatgtttca gttctgcatt tagacgttct
    39481 tcggctctta aaaactgaca gacttccttt tcagattgat tgacatactt ccattctccc
    39541 gacaacaatt cattgggcgt aacgtccagc acttccatta tcttttccag cgtttcatag
    39601 gcaggatagt tcaaccctct ttcaatcttg gaaaggctct gcatattgat accgattttg
    39661 tccgcaagtt cctgttgttt cattccccta aattttctta tggtctgtat attatttcct
    39721 aaatggctta ttttcattgc ataaccctcc atatctcgtt atatcttcgc tattataagt
    39781 ctatcatgct taatatgatt tgtaaaggca aaaaaagata ttgacaaata agcatataag
    39841 aattactatg ttcttgtaat cgaataagca caatagaatg aaatataaaa acattcaaaa
    39901 agaaatacct atctgattaa atccgtagat atgaatatcg gttgaaatgg aacttgttag
    39961 aaaaggcaaa acagaacttg ctgacaaagt atttctgaca cggtggcgga agcctctcag
    40021 gcttaccgcc atgacacctt gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg
    40081 cgagccttgt ctgtgccccc gttatctaac aggggggcac tatttcacaa aagacaagtc
    40141 aaaaaccata caatcccaaa gttgtgagta gtaatgagaa gttttaagaa gatagtgcat
    40201 atcacacttt ttgaatagag gtttacaggt atttcaaggc tattgtggga ttggaggaat
    40261 acaatgaaca atttagaatt tgcacttgaa atgaagaaca aacgtcttgc aagcggtctt
    40321 tcacaaggag aacttgcaag tcttacccat gtatcaagat atagtatcaa ccgctttgag
    40381 aacggaaaag ccaacgccag caaggaaaca caaaatatta tcctgcgttg tctgaattat
    40441 tatatctgcg ataagccttt ttatctgctc gttgattatc tgtctgtccg ttatccaacg
    40501 actgacgcat tggaaattat ccgaaaggtg cttggcatga aagcagatta ttttatccat
    40561 tatgactatg ggtattatgg ctataaagag cattacgcct acggagaaat caaggtcatg
    40621 gcttctgatg acgaacacat gggcgtattc ttggaattaa aaggggcagg ctcacgcaac
    40681 atggaatatg tcttgcaggc acagaacaga gattggtatt catttttaaa ccgctgtctt
    40741 gactgtgacg gtgttatccg acgttttgac ttggcaatca atgatatgtg tggtctgttg
    40801 gatattcccg ttttgtctga aaaatacaga aatggcggtg cggattgtcg ctgtaaaaat
    40861 tatgaaaatg tgcagggtgg aaaactaagc ggaaaaaacc gtaatttagc aagcaccctt
    40921 tatatcggtt caaaggcaag cacaaaatac ttctgcctgt atgaaaaaca aaaggaacag
    40981 gcaaccaaaa agaaacatac ggatattatc aatcgttttg aaattcgtct gcgtggtaca
    41041 aaagcagtac aggcagttga ggaattgtta ttgacatata accctcatgg gctggtgttt
    41101 taccttatta ctgattttgt gcaatttccc gactatccgc tgtgggagat attcatttcc
    41161 catgacagtt taccttttga aatgaacccc gtacctgtca atatggaacg cactctgcaa
    41221 tggctggaaa gacaggtcat gccgtcaatc gtgatgatag aggaaatcga caggctgaca
    41281 ggctcaaact acatgaaaat gattgatgaa tgtacccacc tctctgaaaa gcaggaaatg
    41341 cttgtggaac agatgtgtac ggacatagca gacgtaatcg aaagtgaggg ggtgttttat
    41401 gagtaatgca caggatattc ccgtatggga aaaatacacc cttaccattg aagaagcgtc
    41461 aaagtatttc cgtatcggag aaaacaagtt aagacgattg gcagaggaaa acaaggacgc
    41521 tggctggctc attatgaatg gcaaccgcat acagattaaa cgccgacagt ttgaacaggt
    41581 cattgataaa ttggacgcta tctaatgcaa atgagccttg tatgtgttat gatgaacaca
    41641 agtcatatca aggctctttc caacaaggaa aggagcagac accatgaaag aaaaaagacg
    41701 ggataacaaa ggtcgtatcc tgcatactgg agagagccaa cgaacagaca gaaaatactt
    41761 atataaatat gtagacgcat ttggaaacac aaaatatgtg tatgcttgga gattgacacc
    41821 cacagacccg acaccaaagg gaaaacggga aaaaccctca cttcgagaac tggaacagca
    41881 gataagacgg gatattgagg acggtatcga cagcactggc aagaaaatga cgctttgcca
    41941 actttatgcc aaacagaacg cacagagggc aaatgtgaag aaaagcacac agaaacaacg
    42001 ggaacaactc atgcggttat tgaaagagga caagttaggt gctaggagca ttgatacaat
    42061 caaaccctct gacgctaagg aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa
    42121 taccattaac aaccataaac gctcgttaaa agcgtcattc tacatagcca ttcaagatga
    42181 ttatgtgagg aaaaatccct ttgatttcaa gttgagtgaa gtcctagaaa atgataccaa
    42241 aaagaaagtc gcattgacag aggaacagga acaagcctta ctctcattca tcaagacgga
    42301 caatgtgtat cacaagcatt atgatgatgt gctgatactg ttaaagacag gacttcgtat
    42361 ttcggaactg tgcggactga cagtagccga cattgatttc aagaatgaag ttgtgattat
    42421 cgaccaccag ttactaaaga gcaaggaaca gggctattat attgaaacgc ctaaaacaaa
    42481 aagcggaata aggcaagtac cattaagcaa agaaacgata caggcatttc aacaggttat
    42541 gaaaaaacgt ccaaaggcag aaccatttgt gatagacgga cggggcaact tcctatttgt
    42601 caatcataag ggcaagccca aagttgcgat tgattataac gccttattta tccgtatggt
    42661 aaagaaatac aacaagcacc acaaggacaa tccattgcca catatcacac cgcatacgct
    42721 acgccataca ttctgcacaa gactggcaag caagaacatg aacccaaaag atttacagta
    42781 tatcatgggg cattcaaata tcagtatcac aatgaactgg tatgctcatg cgtccataga
    42841 taccgcaaaa tcagaggttc agcgtctaat tgcatagaag tatttaccac gattttaacc
    42901 acgcttgata gtgaaaatat aagaagatag acctagatat gtgaggttta ccacaaaagc
    42961 aaaatgcccg tagagcctat aaaataaggc tttgcggaca tttaagaaga tataaaaaga
    43021 tagtcaaaaa gacatatata atttatttta aatatttaat actttattca agaaatcttt
    43081 tgtccgttca ttttgcggat tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt
    43141 tccgtcgtca atgaacatga cgcgattggc cacttcttta gcgaacccca tttcgtgcgt
    43201 aacgataacc atcgtcatac cttgttctgc taactttttc atgacgttca agacatcacc
    43261 gaccatttca ggatcgagtg cagaagtagg ctcatcaaaa agcatgatgt cagggttcat
    43321 cgctagagca cgagcaatag ccacacgctg tttttgtcca ccagaaagca tttcaggata
    43381 catatctttt ttatcttcta gaccaactgt ctgcagtaat ttgattgctt ttttctctgc
    43441 ttcttctcta gattctttct tcaggtccat cggtgctaaa atgatgtttt ccaaaataga
    43501 aagatgaggg aataggttaa agtgttggaa taccattccg atatgttgtc tcaccaagtt
    43561 gatatctgtg cttttatcca tcaagtgggc gccgtcaata ataatttcac cgtcagaagg
    43621 ttcttctaaa cgattcaagc aacgcaagaa tgtacttttt cctgaacctg aagggccaat
    43681 gatacagacg acatctcctt cgttgatgga tacattgatg tcgtttaata ctgtgttatc
    43741 gccgtatttt ttgactaaat gctcaactaa gattttttct gccatcttac ttcaccttct
    43801 tttctaaaac ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa
    43861 tgatcaagta aacatacgta ctttgtgttg tacgtgcaac aatgatctta ccagtttgca
    43921 gcaattcgac tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag ataacgaact
    43981 gattgacgaa tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg
    44041 tccgattata agaaagacct aaacttctgg aagcttccat ttgtccgaca ggaacggcgt
    44101 tgatcccgcc tcgaacaatc tctgcgatat aagcacttgc gttaagagtc aaggtgataa
    44161 caccagcaag gaagtcagga atcgtaaatc caacgatacc aggcaaccca aagaaaataa
    44221 agaacgcaag aaccatcatt ggaattcctc ggatgatatc gacataaata ctagcaattg
    44281 tgcgaagagc gcgtattggt gctacactga ataatccaaa aatcacaccg attactatag
    44341 ccaaagcaaa ggagatcaaa gttaggagaa ttgttctcca aagtcctttc aacaatgctt
    44401 gatagttgtt ttggataagt ccaagaaatg ttgattcatc agcagtggaa gactcactgg
    44461 aagtttctga accatccgct acatattctt taataatctt atcgtattcg cctgttcttt
    44521 ttaattcttt caatccttca ttgaacatat taagaagctc aggattttgt ccttttttaa
    44581 cggcaaatgc atattttccg ccagattctt ttttgatcgg tgtttccaat tcttgtcctt
    44641 gtttgatcgc ataaccgatt accggataat catccatcat tgcatcgatt gcgccaactt
    44701 ttaacccgtc gtacaattga tcagcagcat caaaatattt gatagaatag ccatattcag
    44761 attcatggtc gcttaaaaat tcagcacttt cggtaccaac ttttgctccg actgtttttc
    44821 ctttcaaatc ttcataagac ttgatcgaag tatctccttt tttgacagct aattggatcc
    44881 cgctatcaaa gtaagaatca gaaaaatcat agcttttctt tcgttcatct gttacagtca
    44941 ttccggcaat cattccgtct gcttgtcctg attccaaagc ttgcaaagca gcagagaaac
    45001 cacggtagtc cattcggatg ttaaatcctt gcagtttggc gatgttttcc atcaatttta
    45061 catcgatgcc ttcgtactcg ccctttgcgt tttgaaattc aaatggagca aaagcagagt
    45121 cactggcaat tacgtatacg tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc
    45181 ttgactctgc cgttgtagtg ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa
    45241 gtcctgtcat taagaaaaca aatgagtgaa ataaatgctt tcttttcata atcaacctcc
    45301 taaatttcca atattttatt attataggcg aaaagtgctg aatcagacaa gactgtaagc
    45361 tcgaaaaaat gaaaaaaatg cgagtttcta acattttatg ttataaaaca taacatgtat
    45421 aataagataa aaaagtggcg aaagtatgtt cggtgtggta taatttttta gtaaggaatg
    45481 cctttgcttt ccttttcttt ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg
    45541 acgaagaatg attgagagag atacctcccg acactttgag cttgtctcga aataccaacc
    45601 ggctggtgat cagcctgaag cgattaacca attagtggat ggcgtagtcg gcgggaaaaa
    45661 agcacaaatc ttattaggtg cgactggaac agggaaaaca tatacgattt ccaatcttat
    45721 tgagaaagtg aataaaccaa cactgattat tgcccataac aaaacattgg ctggtcagct
    45781 gtacggggaa ttcaaggaat ttttccctaa caatgctgtt gaatattttg tttcttatta
    45841 tgattattat cagcctgaag cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc
    45901 aagtgtaaat gatgagatcg acaaattacg acattcggct actagttcat tattagaaag
    45961 aaatgatgtg atcgtcattg catccgtttc atgtattttt ggattaggct cgccttttga
    46021 gtaccaaaaa caagtagttt cgattcgaca gggagcagaa ctagatcgca atcagttgat
    46081 tcgtgactta gtcagtatcc aattcgaaag aaatgatata gattttcagc gcggtcgttt
    46141 tcgagtgcgc ggtgatgtcg tggagatttt tccggcttca agagatgaac gtgccttacg
    46201 tgtggagttt tttggagatg aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga
    46261 agttttagga gaaacggaac acgtagcaat cttcccggcg actcactttg tgacgaatga
    46321 tgaacatatg gagcatgccg tagcgaatat caaagaggaa ttagaacagc gtttaactgt
    46381 cttgcgaaat gaaaacaaac ttctggaagc tcaacgttta gaacagcgaa ccaattacga
    46441 tattgaaatg atgttagaaa tgggctacac gtcgggaatt gaaaattatt ctcggcatat
    46501 ggacggacgt aaagaaggtg aaccgcctta tacgctattg gatttctttc ctgaagattt
    46561 tttgatcgtg gcggacgaat cgcacgtaac catgcctcaa atcagaggaa tgtataatgg
    46621 tgaccgagcg agaaaacaga tgctagtaga ttacggtttc cgtttgccat ctgctttaga
    46681 caatcggcca cttcggttag aagagtttga aaaacatgtc aatcagatca tttatgtctc
    46741 tgcgacgcca ggaccatatg aacatgaaca aacagatacc gttatccaac aaatcatccg
    46801 tccgacaggg ctgcttgacc cagtgatcga agttcgtcca atcatgggac aaatcgatga
    46861 tctagtaggc gaaataaatg aacgagtcga aaaagaccag cgggtgtttg taacgacatt
    46921 gacgaaaaaa atggcagaag acctcacaga ttatttcaaa gaattaggga tcaaagtcaa
    46981 atatttgcat agtgatatca aaacgctgga acgaacagaa atcattcgag atcttcgatt
    47041 aggtgaattt gatgtgttag ttggaatcaa tttgcttcgt gaaggattag acgtacctga
    47101 agtttccctt gttgctatct tggatgcgga taaagaaggc tttttacgta gtgaacgttc
    47161 cttagtccaa acgattggac gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc
    47221 agacaaagta acagattcta tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat
    47281 tcagcagaag tacaatgaag aacatgggat cgttccaaaa acgatcatta aagaaatccg
    47341 agatttgatt tctattacga aagaatcgga agatgataca aaagaagcgg tacaagtctc
    47401 gtacgaagaa atgacgaagg aagaaaaaga cacgttattg atgaaattag aaaaagaaat
    47461 gaaagatgcg gcaaaagctt tggattttga aacagccgcg aatgtgcgag atatgatttt
    47521 agaattaaaa gcttcaaagt aggaggacaa catggctaac gataaaatta cgattcatgg
    47581 tgcacgtgcc cataacttga aaaatataga tgtaacgatt ccacgagata aattcgtggt
    47641 cgttacaggt ttgtccggtt ctggaaaaag ttccctagca tttgatacgc tgtacgctga
    47701 agggcagcgg agatacgtag agagcctttc tgcttatgct cgtcagtttt taggacaaat
    47761 ggataagccg gacgtagata gcatcgacgg attgagtccg gcgatttcta ttgatcagaa
    47821 aacaacaagt aaaaatcctc ggtcgacagt aggtactgtg acggaaatca atgactattt
    47881 acgcttgttg tttgcacgtg taggacatcc gatttgtccc aacgatcata tcgagattac
    47941 cagtcaatca gtagatcaaa tggcagatca agtaatggct ttgcctgagc gcacaaagat
    48001 ccaagtgttg gcaccgatcg tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg
    48061 aatccaaaaa gagggatacg tccgcgttcg agtcgatggt gagatctatg atttaagtga
    48121 agtgcctgag ctggagaaaa acaaaaaaca tgatatcgcg atcgtcatcg accggatcat
    48181 tgtgaaagaa gggattcgtt ctcgcctatt tgattcttta gaggcggccc tgcgattagc
    48241 agacggctat gctctggttg acgtgatcgg tgaagaagaa atgttgttta gcgaacacta
    48301 tgcctgtcct tattgcggat ttactgttgg cgaactagaa ccaagactgt tctcattcaa
    48361 tgcgcctttt ggagcttgtc cagactgtga tggtttaggt gtcaaattag aagtcgatca
    48421 agatttagtg attccagata gaacgaagac acttagtcaa ggtgctatcg ttccatggaa
    48481 tccaatcagc tctcagtatt atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat
    48541 cgatttagat acaccattcg aagaattacc ggaagagcat caggagatca tcttgaatgg
    48601 gacagatgat cttttccatt tccattatga aaatgatttt ggtggcgttc gcgatgtaga
    48661 aacgactttc gaaggggtat tgaagaatat cgagcgtcgt tatcatgata cgaacagtga
    48721 tttcacgcgt gagcaaatgc gtctttatat gacagaatta acttgtcaga catgtaaagg
    48781 ttatcgattg aatccccaag ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac
    48841 aaatgagttg tctattcaaa aagcagtaga tttcttctca aacttaacgt tatctgaaca
    48901 agagaaagta attgccaaac cgattttaaa agaagtaaat gacagacttt cattcttgga
    48961 aaatgtggga ctagactatc taactttgag tcgcgcttcc ggaacattat ctgggggaga
    49021 agcgcagcga atcagattgg ctacacaaat tggatcgaat ctttctggag ttttgtatat
    49081 tttagatgaa ccatcgatcg gattgcatca acgcgacaac gatcgactga ttggctcatt
    49141 aaagaaaatg cgtgatcttg gcaacaccct cgttgtagtc gaacatgatg aagatacgat
    49201 gtgggcagca gattatttga ttgacgtcgg acctggggcc ggagaacaag gcggacaaat
    49261 cgtggctgca ggcactcctg aagaagtagc taatgatgaa aattctttga cagggaaata
    49321 tctttcaggg aaaaaatcaa tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc
    49381 aatcaagatc actggtgctt cagaaaacaa tctgaaaaat attagtgttg aatttccatt
    49441 aggagaattt gtagcagtca caggtgtgtc tggttctgga aagagtacat tggtcaatag
    49501 tatcttaaag aaatcgttag cgcaaaaatt aaataagaat tctgctaagc caggtaaatt
    49561 caagacaatt tccggctacg aaagtatcga aaagatcatc gatatcgatc aaagcccaat
    49621 cggccggacg ccgagaagta atccagcgac ttatacaagt gtatttgatg atatccgtgg
    49681 gttatttgct caaacgaacg aggcaaaaat gcggggttat aagaaagggc gttttagttt
    49741 caacgtaaaa ggcggtcgtt gtgaagcttg tcgcggggat ggaattatta agatcgaaat
    49801 gcactttttg cctgatgtct atgttccttg tgaagtatgt catggtaaac gatataattc
    49861 tgaaacatta gaagtgcatt acaaaggaaa aagcattgct gatattttgg aaatgacagt
    49921 agaggatgct gtagaattct tcaagcacat tccaaagatt catcgcaaac tgcaaacgat
    49981 tgttgatgtt ggcttaggtt atgtgacgat ggggcaacca gcaacgacat tgtccggtgg
    50041 tgaggcacaa cggatgaaac ttgccagtga attgcacaaa atctctaatg gaaagaattt
    50101 ctatatacta gatgaaccaa cgacaggact tcatagcgat gacatcgccc gcttgttgca
    50161 tgtattacaa agattagtag atgctggtaa cacagtttta gtgattgaac acaatctaga
    50221 tgtaatcaaa acagcagatt atatcattga tttaggacca gaaggtggag aaggtggagg
    50281 aacgatcctt gcgactggaa caccagaaga aatcattaac gtaaaagaaa gttatacagg
    50341 tcactatttg aaaaaaataa tggtataaag aggatagata gctgagatag ttaaaaaaac
    50401 ataaaaaaat gaatttttgc ttgttttatt aatatcagtg ttctagaatc attacatggt
    50461 agttctttct acactgattg gtatagataa aaacttccta taagttcaat ccgttcaaaa
    50521 aagaattgag caatcggagg actttccttt gattgctttt ttctttatgg tatgatattc
    50581 cttgtgtagt tggaaaagaa aaacaagaga ttatgagtaa gtgagagata atgatgaaca
    50641 gtattgaaaa tgaagaattt atccctatat tattaggaag cgacatgaac gtttatggta
    50701 tggcacgttc ttttaatgaa gcatacggca aaatctgcca agcatatgct tcggatcagt
    50761 tagcccctac ccgctacagt aaaattgtca atgtagaagt tattccagga tttgataaag
    50821 accctgtatt tatcgagacg atgcttcgtt tggcaaaaga aagatattct gataagagta
    50881 aaaaatatct tttgattgct tgtggtgatg gatatgcaga attgatctcc caacataaac
    50941 aagagctgtc agaatatttt atctgtccat atattgatta ttctttattt gaaaggctga
    51001 tcaataaagt aagcttttat gaagtttgtg aagaatatga tcttccgtat cctaaaacat
    51061 tgatcgtcag agaagaaatg ttagtaaatg gacatttgga acaggaattg ccttttgaat
    51121 tcccagttgc tttgaaacct gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa
    51181 gaaaaaaagc ttttatttta gagactagag aagagttcga tttaatttta ggccggatat
    51241 acgaagccgg ttataaaagt gaaatgattg tacaagattt tatcccgggt gatgatagta
    51301 atatgcgtgt gctaaatgcg tatgtagatg aagatcatca agttcggatg atgtgccttg
    51361 gtcatccact attagaagat cctacaccag catcgattgg taattacgtt gtgatcatgc
    51421 cagattataa cgaaaaaatc tatcaaacca ttaaggcatt tttggaaaaa atcgaatata
    51481 caggctttgc caatttcgat atgaagtatg atcctagaga tggagaatat aaattatttg
    51541 aaatcaattt gagacaaggt cgtagcagtt tctttgtgac attgaacggc ttgaatctgg
    51601 ctcgttttgt cacagaagat cgagtgttca acaaaccctt tgttgaaacg acctatggaa
    51661 cgaatcaatc agataaagca agattatgga tgggtgtgcc caaaaaaatc tttttagaat
    51721 atgctcggga aaatgaagac aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg
    51781 gtactactgt attttatgaa aaagaccgat caatcaagcg ctggttgttg atgaaatata
    51841 tgttccataa ctatattcca agattcaaaa agtattttca tgtgaaagaa ggctagttca
    51901 tggagaattt tttcagtatt ttaggcggaa tgggcacgat ggcgacagaa agttttgttc
    51961 gcttgatcaa ccaccgaacg aaagctacaa aagatcaaga atatttgaat tatgtcttat
    52021 tcaatcatgc aacagttcct gatcgtacag cttatatttt agatcgatca gaagaaaatc
    52081 caatgccgtt tctgctggat gatattgaga aacaaaattt attgcgacca aacttcattg
    52141 tgttgacttg taacaccgca cactactttt ttgaagagtt acaagcggca acggatattc
    52201 ctatcttgca tatgccaaga gaagcagcaa atgaacttgt ccgtcagcat acgacaggga
    52261 gagtagcgat tctaggaaca gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca
    52321 aaaatcttgg tttcgaaaca gtgattcctg atactgcgct tcaagaaaaa atcaattacc
    52381 tgatctatca tgagatcaag gaatcggatt atttgaatca agagctctat tacgaaatct
    52441 tagaggaagc ggtagagcgc ctaaactgtg aaaaagtcat cctaggttgc acagaattat
    52501 ctttgatgca tgaatttgct gaagataatc attacccagt gatcgatgct caatctattt
    52561 tagcggatcg aacgatcgaa cgtgcattag ctgaaagaag cgaggcattg gatacagcat
    52621 cggaaaagta aaaaaattgg atttggggca aatatcctaa aagatatttg gtccaaatct
    52681 ttttgtaaaa gaaaaagaaa actggtaatg atacgcccgg gatgatataa ttttattatg
    52741 cacaatgtga gaggggttcg taaatatgac agataatttg caattagtca tcataacagg
    52801 tatgagtggt gcagggaaaa cagtagctat ccaaagtttt gaagatatgg gctatttttg
    52861 tatcgataat atgccgcctc gattgattcc aaaattctgg gaattgatca aagaatcagg
    52921 taaagtcaca aaaatggctt taggagtgga tttgcgttct cgttccttct ttgaagaaat
    52981 ccaaaacatg ctgatcgaaa tcgaaaatac gaacttcatc gacacttcta tcttattttt
    53041 agatgcttca gacgaagaac tagtggctcg ctacaaagaa acaagacgag cacatccaat
    53101 ggcgatggac ggtttgatca ctgaagggat acgtaaagaa cgggcgatac tagacgactt
    53161 gaaagcccaa gcttccgtga tcattgatac gacaacattg acaccacgac aattacgtga
    53221 aaaaatcaat gagtcattca aatcaaaggg agaccaagga tttcggatcg aatttgtatc
    53281 atttggtttt aaatacgggt tgccgatcga ttcggatatt gtgatggatg tgcgtttttt
    53341 gcccaaccct cattatatcc cggaactaag accattgacc ggaaaagatc agccagtcta
    53401 tgattatgtg atgtcctttg atgaaacgca atcattctac cataaatttt tggatcttct
    53461 ggaaactgta ttgcctggat acattaaaga aggaaaaagc agcttgacag ttgcaatcgg
    53521 ctgtacaggc ggtcaacacc gttctgtggc attgactgaa cgtttggcaa atgctttagg
    53581 gaaaaactat aaagtcaatg tgacacatcg ggacaaggat aaaaggaaag agacggtgaa
    53641 ccggtcgtga aaatgaaaac ctaccggata agaaaaccaa agatcgtagt ggtcggcggt
    53701 ggcactggtc tgccagttat cctgaaaagt ttacgaaatc aaggagcaga tatcacagca
    53761 gtagtgactg ttgcagatga tggtggaagc agcggtgcaa tcagagaatc gatcgctatg
    53821 gctcctcctg gtgacttgag aaatgtctta gtggcgttat cggatatgcc acaattctat
    53881 gaagatattt tccaataccg tttcaaaaag gaagatcagt ttttagccaa tcatacgatc
    53941 ggtaatctga tcattgccgc agtttccgag atgcggggaa gcacttatga ggcgattcaa
    54001 ttattagcaa aaatgatgca tgtagatgga catgtttatc cttcttcaga aactcctttg
    54061 accttgcatg ctgtttttaa agacggaacg actgcagtag gagaatctaa aatcgcgatc
    54121 aaccgtaaaa ccattgaccg tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa
    54181 gcggctcgaa aagtcgtttc agcaattatg gacgccgaca tggtcgtttt gggacccgga
    54241 agtttattca ccagtatcct tccaaattta gtgataccag aaatcggcga agcaatgctc
    54301 aagacaactg ctgaaaccgt ctatatctgt aatatcatga cccaaaaagg ggaaacggaa
    54361 catttcacag atgccgatca tatacgtgtt ttacacaagc atttgaatca gccttttatc
    54421 gatacagtat tggtcaatac ggaaaaagtg cctgaagatt atatggaccc agagatctat
    54481 gatgagtatc tagtacaagt cgcacacgac ttcaacggat tgagaaatga aggatgtcga
    54541 gtgatttcca ccgacttttt agaattgaaa gatggtggtg tcttccacga tggagacaaa
    54601 gtagtagaag aattgttccg tttagttttc ggttcaaaat attaagcaat ttattgttgg
    54661 agaaaggagg agaataggat gtcatttgct gcagatgtga agaaagaact aactggtttg
    54721 gaagttcatc gagaacatgc gaaagcggaa ttagctgctt tgctgcgaat gaacggctct
    54781 ttaggtattg tgaaccagca gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc
    54841 agacgtatct attctctttt gaaagatcat tatcaggtac gaagtgagct actcgttcgt
    54901 aaaaaaatga aattaaaaaa gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa
    54961 gatgtgttga tggatcttga tatcatggat ggtatgatgt tcaatggaca cgtttcggac
    55021 gagattatgg gaaattcgca aaaaatgcgt tcttacttac gtggtgcttt tatggcctca
    55081 ggttcagtca ataatccgga aacgagtcgt tatcatttgg aaatattttc tatttatgaa
    55141 cagcataacc aagacatttg cgatatgtta aattattacg gtctacatgc tcgaacgctt
    55201 gaacggcgca atggctatat ttcgtattta aaaggagcag aaaaaattgc tgatttctta
    55261 acattgattg gtgcaaccaa ctcaatgctc cgatttgaag atgttcgaat cgtacgggat
    55321 atgagaaatt cggtcaatcg tctagtcaat tgtgagacag caaatctgaa caagacgatt
    55381 gatgcggctt ctaaacaaat cgaaaacatc gaattgatcg aagcaaaggt cggtctacat
    55441 gcccttcctg aaaaacttca ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc
    55501 ttgaaagaat taggggaaat gattccctca ggtgcaatat caaaatcggg aattaatcac
    55561 cgaattcgca aaataaatga atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa
    55621 gaaaaaactg atacatgaag caggagatgc gaatgtatca gtttttttta aaagctatac
    55681 ttgcttttct tccgtcaaac tataattcca actttactgg agtaacataa tgttgaaagg
    55741 cttctattgt tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt
    55801 agttttatac ataaactttt gcttaatata gaagtcctga tatagatagg atcaagtatt
    55861 tatctataac tggagattaa ttaaaaactg gaaggaactg ctcgatcaac cttgagaact
    55921 gtttttctta cttctggtca agattctcgt ggttggtgga taaggatggt tgataagttc
    55981 attggattag ctagtcgaaa acaaccaggt gtaattaatg tagaagtttt aacaagggga
    56041 tggagtgatt tattcagtga taatcataag ttcataaata gtaattggga tgtatggttg
    56101 gtatacgtta tacctgaact tgaactatca agatttggtt tagaatttga gaatcgatgt
    56161 ttataaaggg agggcaaaaa atgtgctttt ttacaacttt tactatcatt atgattgcag
    56221 tactttttat ttttctagat atagcaaagc gaaatacagc atttttatta tatcgggttt
    56281 tacttcgagc aggtctcata acatttatat cgatcgttgg attttttcta ttcacagtta
    56341 ttgtatttat ttggagaact acagcaccac cattgccaga gattacatat ggagaatttc
    56401 cgtttagtct tgaatatgaa atagatgagg aaagaatttt gattgaagat acggtgattg
    56461 cagaatttat aggaagtttt agagggaatg tgacaactca acctagacga agttggagta
    56521 taaaactttt aaataatagt ggagagttga aagcatttca ttatgaattc ttaataatag
    56581 aaagtgaaga tgtaacaatc actttaagta gtggctctgc tggatatttt atgaatgatg
    56641 gtggaagcaa gccacagctc acagtccgtg atcgtattgc aggttttatg catttttctc
    56701 actatgactt agaagaagct cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt
    56761 gggaatatgc gccaccgata agaaattttt tcaatgatta ttattagtct tataaaaaac
    56821 agaatgtaaa atttatgtgc gcgagtattt agttttccat tttttgtttg atagatatat
    56881 agcattgttg aagcatggga tagtggtgcc aattttatca atgaggttgg tcaggcagtt
    56941 aacataattg gtgatgctat aaatccattt aattggaatt ggaggaatta ggtgtggaaa
    57001 cagtgagatt atggatattc atgaaaaatt ttcgttcggc tttgagatat gttattctgg
    57061 aatcaaaatc aggttattat ctgattgaca cagatcacta tactttttgg ggaggctacc
    57121 tttttccgat ttttaattgg ataatccccc ataaggttat gaaaattaac ttgcctgcag
    57181 aagaaataaa tagattaata ctcaacaaag atttacaaag gaaaagagag aaagaacatt
    57241 ctaatgttgc ttggataggt ccagcatttg cagtagtgac ttcttcaatt gtcggtccta
    57301 tgctagagag tatttatagt gatttttcta ttggaatacg ttatttattt gttactttaa
    57361 gtatagctgt tcttttgtgc ataaagggtt atgttagcaa gaagaataaa gatattttaa
    57421 cactaatcgg tgtaaacaat ttatctagta ctaaaattat actgtttcct gaatctataa
    57481 aagaaggatc aaggttgttg ataattttta taatgcttat actgttttct gttttatttg
    57541 gggtgatgtt tataga
//
LOCUS       DACEDO010000008        56753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEDO010000008 DACEDO010000000
VERSION     DACEDO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.008"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWO52_000540"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWO52_000540"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0354830.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IWO52_000541"
     CDS             complement(937..1518)
                     /locus_tag="IWO52_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0354831.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWO52_000542"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWO52_000542"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0354832.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IWO52_000543"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IWO52_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0354833.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IWO52_000544"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IWO52_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0354834.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IWO52_000545"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IWO52_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0354835.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IWO52_000546"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IWO52_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0354836.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWO52_000547"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWO52_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0354837.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IWO52_000548"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IWO52_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0354838.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IWO52_000549"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IWO52_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0354839.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IWO52_000550"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IWO52_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0354840.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IWO52_000551"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IWO52_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0354841.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNRAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IWO52_000552"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IWO52_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0354842.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IWO52_000553"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IWO52_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0354843.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IWO52_000554"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IWO52_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0354844.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IWO52_000555"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IWO52_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0354845.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IWO52_000556"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IWO52_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0354846.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWO52_000557"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWO52_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0354847.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IWO52_000558"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IWO52_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0354848.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IWO52_000559"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IWO52_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0354849.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IWO52_000560"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IWO52_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0354850.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IWO52_000561"
     CDS             13972..14157
                     /locus_tag="IWO52_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0354851.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IWO52_000562"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IWO52_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0354852.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IWO52_000563"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IWO52_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0354853.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IWO52_000564"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IWO52_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0354854.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IWO52_000565"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IWO52_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0354855.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IWO52_000566"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IWO52_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0354856.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IWO52_000567"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IWO52_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0354857.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IWO52_000568"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IWO52_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0354858.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IWO52_000569"
     CDS             18232..18879
                     /locus_tag="IWO52_000569"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0354859.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IWO52_000570"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IWO52_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0354860.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWO52_000571"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWO52_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0354861.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IWO52_000572"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IWO52_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0354862.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IWO52_000573"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IWO52_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0354863.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IWO52_000574"
     CDS             20317..21255
                     /locus_tag="IWO52_000574"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0354864.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IWO52_000575"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IWO52_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0354865.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IWO52_000576"
     CDS             21935..22774
                     /locus_tag="IWO52_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0354866.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IWO52_000577"
     CDS             22750..23619
                     /locus_tag="IWO52_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0354867.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IWO52_000578"
     CDS             23609..24406
                     /locus_tag="IWO52_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0354868.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IWO52_000579"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IWO52_000579"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0354869.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25810..28479)
                     /locus_tag="IWO52_000580"
     CDS             complement(25810..28479)
                     /locus_tag="IWO52_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0354870.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28717..28905)
                     /locus_tag="IWO52_000581"
     CDS             complement(28717..28905)
                     /locus_tag="IWO52_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0354871.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29175..29537
                     /locus_tag="IWO52_000582"
     CDS             29175..29537
                     /locus_tag="IWO52_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0354872.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29589..31271
                     /locus_tag="IWO52_000583"
     CDS             29589..31271
                     /locus_tag="IWO52_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0354873.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31388..33424
                     /gene="recG"
                     /locus_tag="IWO52_000584"
     CDS             31388..33424
                     /gene="recG"
                     /locus_tag="IWO52_000584"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0354874.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33473..34474
                     /gene="plsX"
                     /locus_tag="IWO52_000585"
     CDS             33473..34474
                     /gene="plsX"
                     /locus_tag="IWO52_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0354875.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34596..34838
                     /gene="acpP"
                     /locus_tag="IWO52_000586"
     CDS             34596..34838
                     /gene="acpP"
                     /locus_tag="IWO52_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0354876.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            35106..36110
                     /locus_tag="IWO52_000587"
     CDS             35106..36110
                     /locus_tag="IWO52_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354877.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            36111..37055
                     /locus_tag="IWO52_000588"
     CDS             36111..37055
                     /locus_tag="IWO52_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354878.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            37055..38017
                     /locus_tag="IWO52_000589"
     CDS             37055..38017
                     /locus_tag="IWO52_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0354879.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            38044..38958
                     /locus_tag="IWO52_000590"
     CDS             38044..38958
                     /locus_tag="IWO52_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0354880.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38984..40765
                     /locus_tag="IWO52_000591"
     CDS             38984..40765
                     /locus_tag="IWO52_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0354881.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            41113..41799
                     /locus_tag="IWO52_000592"
     CDS             41113..41799
                     /locus_tag="IWO52_000592"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0354882.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41819..45400
                     /gene="smc"
                     /locus_tag="IWO52_000593"
     CDS             41819..45400
                     /gene="smc"
                     /locus_tag="IWO52_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0354883.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45409..46218
                     /locus_tag="IWO52_000594"
     CDS             45409..46218
                     /locus_tag="IWO52_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0354884.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46229..47227
                     /gene="ftsY"
                     /locus_tag="IWO52_000595"
     CDS             46229..47227
                     /gene="ftsY"
                     /locus_tag="IWO52_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0354885.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47414..49681
                     /gene="gshAB"
                     /locus_tag="IWO52_000596"
     CDS             47414..49681
                     /gene="gshAB"
                     /locus_tag="IWO52_000596"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0354886.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49770..50222)
                     /locus_tag="IWO52_000597"
     CDS             complement(49770..50222)
                     /locus_tag="IWO52_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0354887.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50372..51322
                     /locus_tag="IWO52_000598"
     CDS             50372..51322
                     /locus_tag="IWO52_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0354888.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51315..52274
                     /locus_tag="IWO52_000599"
     CDS             51315..52274
                     /locus_tag="IWO52_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0354889.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52271..53026
                     /locus_tag="IWO52_000600"
     CDS             52271..53026
                     /locus_tag="IWO52_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0354890.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            53049..54002
                     /locus_tag="IWO52_000601"
     CDS             53049..54002
                     /locus_tag="IWO52_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0354891.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54525..54821)
                     /locus_tag="IWO52_000602"
     CDS             complement(54525..54821)
                     /locus_tag="IWO52_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0354892.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            55176..55532
                     /locus_tag="IWO52_000603"
     CDS             55176..55532
                     /locus_tag="IWO52_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0354893.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55542..56441
                     /locus_tag="IWO52_000604"
     CDS             55542..56441
                     /locus_tag="IWO52_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0354894.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        18625 a        10248 c        12168 g        15712 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccgtgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtttttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaaggaag aggtcgtgaa atcagcgttt
    25501 tgacctgagt attagagaaa caaacgggaa aatagctcct catattttgc cgtttgttga
    25561 gctaataccg aaggtcagct gattgaacac cgtttaagaa agaggtcgtg agcctgtcat
    25621 ttaactctga gtcacaaaga gcaatttcaa aaaacagctt ctcatctttg atgaatatcg
    25681 tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt ttttctgtaa
    25741 ccaaaaaacg atggaacgtt gttttgattg cccttggtgc ggacagtctg aacaggttcc
    25801 atcgtttctt tattttctac gtcttcttct gtgtgtgttt cgtggttgtt cttctattgc
    25861 tggatcaatc gtgccataaa aggggcgatt gcggtcaagg taccagacat aaagaataaa
    25921 caatgcaata cataggacaa aaaggattgc acctatcgta aatccttcag gcaagtaact
    25981 gaaagtgaca gtatgtttac ctgctggaac ttttatcatc aagaatgcgt ctttgaaatt
    26041 gctgaccttg actggtttcc catcaatttt agctttccat cctttgtcat aaggaatagt
    26101 tgtcaccaac atggaatctt cagcggtatt tacagtccca gaagcttttc tgccagtggc
    26161 ttccatttcg acaccttttt ctttgatctg attcattgcg gcttgatagg ctacaacatc
    26221 tagaccaacg actttaggct ccatgaaact tatttctttc gtcccataaa aacttgcagt
    26281 aaatgtcact gttgtctctt gaggataata gccaagatca tagtattgac cagaaatgtt
    26341 gatttgcgtt tttcgcgatg taccattgac agtgatcgtc gcactagaac tttctaattg
    26401 cgcaaaattg gttgggaaca aacttaagta agcttgtgta tttgcaggca catggacttc
    26461 ccaagtgagc tcttttggct ggttatctgc tgtttctgag taagtgacgc cagtcgttgt
    26521 ctttgtgatc gtcgtattca actgattctt caaagtaggt gtatagaatg taaaatactg
    26581 ttcatttaga ccagacaatg tattgatcaa gtttgtctga ctggtcaaat tatctgtcac
    26641 tggctgctca acatcattaa cggacaacgg tgcagtaaat gctagtggca atgcattgac
    26701 attctgatac agtgtatagt cgccgctttg accagcttgg cggaagccat atttactgaa
    26761 cgtgctttcg tttttagaaa tgttatattt gattcctgta aacccatcca tcagcaaagt
    26821 attattgtta taacggatat tcaaatttgt ccctcttgaa cggaaaccta atttatccag
    26881 gtaagaggaa gaattacgat ttcttataga agaaaacaaa ctgataccac tgtaaccata
    26941 gttgatcgaa tcgtttgcag atactggatc cagattttcc aagcggtaaa acgagtcgga
    27001 atcttttttt gtttcattta caagagcttt caagtcagga taaggatcag tatataaact
    27061 gcgggaagca taattccaat catcaagaat gccatggatc atactgttcg tattgaccag
    27121 tgcttcagca gacatgatca ataataacaa tatactcaga tgattgaatg aaagtttttt
    27181 atgctgataa aatcccacag caaaagcata aatccctaag aaaaccactg tcaatacaaa
    27241 ggaagtcatc gtcacatagg tgtagcttcc ttcaggtttg atgccccatg ccaccgcaaa
    27301 gagagcagct acgaccaaaa tgattcctaa caacagccca cgatcgtctt gttctatctt
    27361 ttcccatcct tttcctgcaa gcaggagcac taagaaagag aacagatagg cataacggaa
    27421 taaaaacata ttcggtgcat gcataccatg ccagaacaaa ttcaatggaa caagataaaa
    27481 acttgcgata agtatggcga aaagactgcc gtacaaaaat ttatttttca acggtacttt
    27541 ccgactggca aaataaaaca gacagaggac taatggtagc agacctgcgt aaacgaacgg
    27601 aatcgatccg tattttgtcg tgtcataaac accaatcata tttttcatga ttagatccag
    27661 ataagaagtc gcttctgttt tcaacgtagt gatctgactc agtgcctcgc catttgaccg
    27721 aagatccagc actgctggca atacaatgat cattgaagcg ccgcctgcca gtaaagaagt
    27781 gataccataa ggaacaatcg tttttttata ttctgaccag tttgtcaata gacgggcgat
    27841 aaaatataag aaagaaaata cgccaatcat aaaacccata taaaaactgg ataaaaacaa
    27901 gagtaaataa ctgataaata aaagtttcgg ccgtttaaga tccataacac gatgaatccc
    27961 caaaatcact aatggaagat aaaaaaatgc atccagccac atgatgatct ccgaatgtgc
    28021 agtgataaat gagatcaatg cgtaactgac gctaagtgca acatgccccc agcgaggaat
    28081 cttgaacgta cgtgcaagaa accaaaaagc caatccggca cagccgacct taagcaaagt
    28141 cagaacatac agtgcatctg gcatcatctg gtttggaaaa aagaacacca gtggcgtgaa
    28201 gatcccgcct agatagtaag aaattaaaga taaataattc aatcctagag aagcattcca
    28261 tgtataaaaa atactttgct ttccaagaag aacatttcgg aagctggcat gaaaattaga
    28321 aaactgtgaa aaggcatcac ttgccaaaat acttctgctg cttccaggat agattccgat
    28381 acttagataa acaagtgcta aaatcaaaaa cggaatcagg aaactagctg ctgcgtagcg
    28441 tccgttttct ctaaagaatg actttaaatt ttttttcata tttattttcc accaaatttc
    28501 tatgaagttg tattcagttt atcatatcct tgtttttctc tgcttaaaaa gagtctagtt
    28561 ttcacaaaaa aataaaattt agaataaata acttcataat ttgttcgtat aacaaaaata
    28621 aacgcaaaaa aagggtagga atcaaaagtc ccacccttaa aaatttttat taaataaaaa
    28681 accgttcact atctgaacgg tccaagtatc attcaattaa acgcgttcaa cttttcctga
    28741 tttcaaagca cgagttgaca cccaaacttt tttaggtttt ccgtcgacca taacgcgaac
    28801 tttttgtaag ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg aacggttgtt
    28861 tccacttttt gtcttgcgac ctgtaaagta acaaactttt gccatggtaa tttcctcctt
    28921 tgccttgatt tggagcaata gaataattca gctcatacca gactaacata tcatatagaa
    28981 tgggaatttg caagtacatt ttcatagata caattgacaa ttgagaagaa taattgtaaa
    29041 ttaaacaagg ttatacctga aattttgcaa ggtttttgag tactttgggt aactgccttt
    29101 tatttcaagt tgcgctatga taaaatatga tagaagaaaa atgggagttt tttacccgtt
    29161 aaggaggctc attcatggct gtaaaaattc aaacgtcagc tggaaccatt gaaattacca
    29221 atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt gttgtaggaa
    29281 tggccagcaa aaatcaaatt aaagataata ttactgagat tttgcgtaaa gaaaattact
    29341 caagaggagt agttgtccgc caagaagata atggtattgc tgtagatgtt tatacgatta
    29401 tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa gtgaaataca
    29461 atctagaggc attattaggc gtaactgcca attcagttaa tgtctttgta caaggtgttc
    29521 gtgtactgcc agactaggca ggagactggc tggtaactca gcagtcgatc tgaaggagga
    29581 ttatctaggt gagtgtaaca gaaataagtg caagccagtt ccaggaaatg gtccaggcag
    29641 gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc attaaatgta ttccctgtgc
    29701 cagatggcga tacgggaaca aatatgaatc tatctatgac tagtggagct aaggcagtag
    29761 tagattcaac ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa ggattgctga
    29821 tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga ttttctaagc
    29881 aaattccaga tgttgtctca ttaaatgctt ctgatcttgc tgcagccttc actcatggag
    29941 ttgaaactgc gtataaagca gtaatgaaac cagtagaagg aacgattttg acagtagcta
    30001 gagaagcagc gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc gttgaagtaa
    30061 tgacagctgt tgtaaaagga gcaaagcgtg ctttagctaa aactccagac ttgcttccag
    30121 tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg actgctgttt gtttatgagg
    30181 gctttttaag tgcattgaat ggagaattcc aagcggatga aacatatgaa ccttctccag
    30241 cagaaatgga tgatatggtc aatgcagaac atcatcgcag tgtccaaggt caaatggcga
    30301 cagaagatat ccattttggt tactgtacag aaatcatggt aaaaatcgga gaaggaccta
    30361 cagtcgatag tacattcgat tacgaagaat tccgtaacta tttagatggt ataggagatt
    30421 ctttacttgt ggtcaatgat gacgagatca tcaaagtcca cgtgcataca gaaaacccag
    30481 gagaagtctt gaattatgga caaaaattcg gttcattagt caaaatcaaa gtcgacaaca
    30541 tgcgcttgca gcatgagacg attttggaac acgatgaaga agtgaacgag tttgcgaaaa
    30601 aagaaatacc acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa ggcgtccaag
    30661 agctgtttga aagtttaggc gctaattatg ttatcagtgg tggacaaaca atgaatccaa
    30721 gtacggaaga cattgttaaa gcgattcaag aagtaaacgc agataaagtg attgtcttgc
    30781 caaacaataa gaacatcttc atggcagctg atcaagcagc agaagtagca gatatcccag
    30841 tagcagttgt tccttcaaga acgatttccc aaggattgac agctatgctt tcattcaatg
    30901 atcaagcttc tttagaagaa aacaaagaaa cgatgacaga aatgttgtct agcgttgtga
    30961 gcggtcaagt cactcacgcg atcagagata cctcaattga cggtgtagag atcaaagaag
    31021 gagacttttt gggcatgatc gatggaaaaa tcctgatctc taacccagat atcacagcaa
    31081 cagcgttaag tacactagaa caaatgatca atgaggacac agagatcgta acgatcttgg
    31141 caggtgatga aggaacagtt gaacaagcaa atgaatttgc tgagaaacta acagaaaaac
    31201 atcctgattt ggaaatcgag atccaccaag gagatcagcc tgtctatcct tatctatttt
    31261 ctgctgaata atttctgtgt cagaatatct ttctaacaca gtattcattc ataaaaggcc
    31321 ttcggttagt gatcaggtat gatcgctgac caagtccttt ttttgttaaa ggagggagga
    31381 aaaatttatg cgttctttag atgatccaat cactatgctg gcaggagtcg gagaaaaaag
    31441 agcagctagt cttgccagct tgggcatcca aacgattgaa gacttactga cttattatcc
    31501 ttttcgctac gaagatattc aggaaagaaa tctaaacgag atccaggatc aagaaaaggt
    31561 tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt ataaaaaaag
    31621 ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt tttttaatca
    31681 accttatcta aaagaaaaag tcattttatc ggaggaaatc gccgtatacg gtaaatggga
    31741 tgcaaagcgt aaagccttga acgggatgaa gattttaggt tctcaaacaa tggatgactt
    31801 ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg acattagttg atctaatcaa
    31861 aaaagggttt caagaatacg gcacattgat tgaagaaaat ctgccaaaca accttgtgga
    31921 gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt atgcattttc caaaagacca
    31981 cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa gagttctttt tatttcaaat
    32041 gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg agatccttta
    32101 tgatgtccaa aaattgaaac aatttactca aaagctacct tttgaactga caaatgcaca
    32161 aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata tgcagcgact
    32221 tttgcaggga gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt tatacgctac
    32281 cgttacagca ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg cacaacaaca
    32341 tatggaaagc ttgcaacagc tgtttgatcc caatgaagta accatcgcat tgattacggg
    32401 gtcaacgaaa gcaaaagaac gacgtgagct ccttgaaaaa ctagagcaag gggaaattaa
    32461 cgtcgtgatc ggcacacatg cattgatcca agatggagtc gaatttcaga atttaggtct
    32521 agttattacg gatgagcagc accgttttgg cgtgaaccag cggaaagtac ttcgtgaaaa
    32581 aggctggcga ccagatgtct tatttatgac agcgactccg attcctagaa cattagctat
    32641 tacagcatat ggtgagatgg atgtatctat cattgatgaa cttccagctg gacgtatccc
    32701 gatcgaaaca cgctgggtaa gaacacctca attggacagt gtactggatt ggacgaaaaa
    32761 agaactggca agaggacatc aaatgtatgt catctgtccg ctgattgaag aatcagaagc
    32821 attagatgta aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat ttgcaccaaa
    32881 atatgaagtt ggcttgttac atggtaaaat gaagaatcag gaaaaagaag taatcatgga
    32941 agccttcaaa gacaaccaga tgcagatatt agtttcgact actgtaattg aagtaggggt
    33001 aaacgtgccg aacgcgaccg tcatgctgat catggatgcg gatcgatttg gattggcaca
    33061 gctgcaccaa cttcgtggac gtgttggacg cggaagtgat gcttcctact gtatcttagt
    33121 tgctaatcct aaaaatgaac taggtgtgga acggatgaaa atcatgacag agaccaccaa
    33181 tggttttgta ctaagcgaaa aagatctgga attacgtggt cctggtgaag tatttggctt
    33241 ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg gtgacggatg gaaatgtttt
    33301 ggaggtagcc agagaagagg ctgcagccct ttggcaaata aaggattggc agatgcttcc
    33361 tgagtatcag cctttagcgc aaagcctcca aatagaaaat caggataatc gttatttcga
    33421 ttagcagact gtgctatact ttatactgat gacggaaaaa ggagtggaaa caatgaaaat
    33481 tgccgtagat gcaatgggcg gagatcacgc acctcaagca attgtagaag gcgtggcttt
    33541 agcgcagcaa gattttccag aagtagaatt cttactttac ggaaaagaag cagaaatcaa
    33601 gaaatactta acaaatgaaa aaaacatcac gattatccat acagacgaaa aaatcaatag
    33661 tgatgatgag ccagtaaaag caattcgacg aaaaaagact gcatcaatgg tccttgctgc
    33721 acaagcagtg aaaaacggtg aagcagatgc tgtattttca gcagggaata caggcgcgct
    33781 actagcagca gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc ctggcttgat
    33841 gtctaccttg ccagtagtag ggaaagaagg tgccggtttc gatatgctgg atcttggcgc
    33901 taacgcagaa aacaaaccag aacatttgtt gcaatacggt attttaggtt ctttttatgc
    33961 acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg gaacagaagc
    34021 aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg aaacaagctt
    34081 gaattttatt ggaaacgtag aagctcgtga gctactcaat ggtgtggctg atgtagtggt
    34141 cacagatgga tttacaggca acgctgtttt gaaatctatt gaaggaacag caatgaatat
    34201 gatgaatcta ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa tgggcgctat
    34261 gcttttaaaa gacggcttac gttctttgaa agcagaaatg gattattcca aacatggcgg
    34321 tgccgttttg tttggcttga aagcaccagt cattaaaact catggagcaa caggccccga
    34381 tgctgttcgc tatacgatcc gccaaatcca cacaatgctt gagacagatg tagttggaca
    34441 attagtcgaa caatttgaaa aaagcgaaga atagtatttg gatatctgaa gcgatccgtt
    34501 ccattgaaaa aaaggtggac attcctgaaa aatgccagta aaattagcca tggtgtaggt
    34561 aattgacaag tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa gtatttaaca
    34621 aagtagcgaa aattatttca aatcattttg agatagatac cgataaagtg acggatgaat
    34681 tgaacatcaa agatgattta aaagctgatt caattagtat tatggaattc gttttagagc
    34741 ttgaagatga attcggtacg gaaatttcag atgaagacgc tgaacaaatt gaaacagtcg
    34801 gtggcgcagt agattatatc agcagccatc ttaaataatt tcaaataaaa gcaaagaagg
    34861 ataaaaagct gcatgcagct ttttatcctt ctttttttaa tctttttgac acgtaaaagt
    34921 catttttctg ataattctgg cgataaacag aaattatgtt gcaaagtaaa gcggttttct
    34981 atataattat taataattca ttacacaaag ataagaataa cctttgttat aaaacgattt
    35041 attctgtgta atgtcctttt ttattaagaa aaacttccat ttaggaagca gagaggggta
    35101 ttcgattgtc cgataaccaa ttattagaag ttcagaacct acatacagga ttccgactca
    35161 aagatgacta ttacgatgca gtagatgacg tttcatttac tttagacaaa aacgagattt
    35221 tagcaatcgt tggggaatct gggtgcggga aaagtacctt ggctactacg attatgggat
    35281 tacttgatcc taataacaca aaaattacag gtgagatttt atataacgat ctgaatttaa
    35341 tcgacctgaa cgaaacgctc tataataaaa tccgcggaaa tgatatcgga atgattttcc
    35401 aagatccgtt gtctgcatta aatcctctca tgagaatcga agaccagatc aaagaaggac
    35461 tttcctatca cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta gaattgctag
    35521 aacaagtagg gattcctaat cctgcgagag ttggacgcca atatccccat gaactatcag
    35581 gcggtatgag acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct ccaatcgtca
    35641 ttgcagatga accgacgaca gccctagatg taaccatcca agcacagatt cttgatttac
    35701 tgaaagacct tcaggaagaa acaaaaacag ggatcatctt gatcactcat gacttaggag
    35761 tcgtagcaga aatggctgat cgagtggcag tgatgtatgg cggacagttc gttgaagtag
    35821 caggtgtaaa agaactgttt gatcatccta agcatcctta tacacgctct ttactgaact
    35881 ctataccaca agaaggaaat catgatgcag aactccatgt gatcgaaggt gtcgttcctt
    35941 ctctgaaaaa catgcctcgt aaaggttgtc ggtttgcacc acgtattcca tggataccag
    36001 caagcgatca tgaagaagag cctatccttc atgaagtcgc accgaaccac ttggtacgtt
    36061 gctcctgcta taagcatttc cactttagag acgaaaaagg agaggtgtag atgacagaat
    36121 taatcacaat caaagacctg aaagttcatt accctattcg cagtggattc ttcaatcgag
    36181 taacggatca tgtatatgct gtagatggtg tggattttat tatcgaaaaa ggaaagacat
    36241 atggtttagt gggagagtct ggctctggaa aatctactac gggaaaagcc gttgtgggat
    36301 tagaaaaggt aacagccgga caaatcatgt atgaaggaaa agatgtaacg aagcgaagca
    36361 accgcaaaaa aatgggttat aacaaggatg tgcagatgat cttccaagat tcaatgtcca
    36421 gtttgaatcc taaaaagcga gtgttggata tcattgctga acccatccga aattttgaac
    36481 ggttaagcga tcaagaagaa aagaaaaagg taaaaggact gctagatatc gttgggatgc
    36541 cggaagatgc gctatataaa tatcctcatg aattttcagg cggacaacgt caacgtctag
    36601 gagtagcccg tgctgttgct accaatccaa aactgatcgt agccgatgaa cctgtatctg
    36661 ctctggatct atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc cagcaagaat
    36721 acggcctcag ctatttattc atttcacatg atttaggtgt agtaaaacac atgtgcgaca
    36781 atatcgcaat tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa gacatttata
    36841 atgacccgcg acatatctat acgaaacgtc tgctttcagc tattccgcaa atcgatgtag
    36901 aaaataggga aagccacaaa gaaaatcgac gaagagtaga acaagagtat atccaaaacc
    36961 aaaaagaata ctatgatgcg actggtcgag tctacgattt gcgtacgatc acaactacac
    37021 acaaagtggc gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc tgcgacgtgt
    37081 tttattgatg atcccgcaag ttattatttt aagtgttctg atcttcttat tagcccaagc
    37141 gatgccgggg gatccattta ccggtttgat caatccaaat caggatccgg cagtgatcga
    37201 acagatgcga caagcagctg gcttgaatga cccatggtat gtccagtact tccgttggat
    37261 cggaaatgct ttgcaaggcg attttggtca aagtttctta tacaaacgct ctgttgctga
    37321 cttgatcggc gaacggatca tcaacacttt atatctatcg atcttgacag ttgtccttac
    37381 ttatttgatt gctgtgcctc tggggatgct tgctggccgg tatcaaaatt caatcttgga
    37441 taagattgtc gtaatctaca acttctttag ttttgctgtt cctttattta tcttcggctt
    37501 gatcatgcta tttgtttttg gttatcgttt aggctggttc ccaacaagtg gatcacaaac
    37561 actaggtttg caaggtggat ttatcactca atggatagac cgtttgaaat atattctgtt
    37621 accatcaatt acccaagcat ttttggcgac tgccgtcaca atccaatatc tgcgaagtga
    37681 agtaatcgat tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag gggtaccgac
    37741 gaataaagta ttcagtcgac atattttccg aaatgcagcg ttgccgatgg cttctcaaat
    37801 ggggtatgaa atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga tttttggtta
    37861 tccgggagtc ggaaaactat ttattgattc catcggccaa cgggactata ccgtgatcac
    37921 atccttagta ttgattttag gaatcgcgac gctagtgggg acattacttt cagatatcat
    37981 catgagtatc gttgacccgc gtattcggat tcaataaaac caatcagaaa ggagaatgga
    38041 tcgatgagtg aaaaaaacaa tacagcagtt caagaaagta ttcctcctat ggggatacga
    38101 atgattgctc gagaatttaa aaaagataaa attgctattt tttcattagg tttgctagtg
    38161 gttttattat tagtgatctt tatcggtgcg atgctgaccg atcaagacaa agtgatgtat
    38221 gttagtatct tcgacaaata cgctgcacca ggaacagttt cgacacaagg acaaaagttc
    38281 attctgggag cagatgaagg cggacgtgat gtattaggac aattgatcat aggtgctaga
    38341 aactctgtta cgatcggatt tgcgatcacg atccttactt cgatcatcgg ggttggatta
    38401 gggatcatct ctggctacta cggagggatc attgacaaca tcttgatgcg gatcgttgat
    38461 tttatcatga ttctaccgat catgctgatc attatcgtct tcgtatcgat tatttccaga
    38521 tacaatgtct ggtcctttat attcatcatg agtgcatttt attgggtagc taaagcccga
    38581 ttattccgaa gcaagacatt atctgaagca agaagagatt atgtcagtgc ctcaaaaaca
    38641 atgggaacca atgattttaa aatcatgttc cgcgaaatca tgccgaatct aagttcatta
    38701 atcattacta atttgacgat gaactttgcg gcaaacatcg gtatcgagac aactttgact
    38761 ttcttaggtt tcggcttgcc ggcaaatgta cctagtttag gtacattgat cggttatgca
    38821 agtaacggag aagtcttagt gaatcggcaa tggatctggt tacccgcatc aatccttatt
    38881 ttagttttga tgttgagtat caattatgtt ggacaagcat tcaagcgttc tgcagatgcg
    38941 cgccaacggt taggctagta tagaagcgaa gggggaaata ggaatgaaga aaacgatttt
    39001 tggatttgca gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg gaggtacaga
    39061 tacctctgga aacaactctg gaggcaacgg caataacaaa gaggaattaa cttttccaat
    39121 tgcaacaaaa aataatgatg atgcaatcga agacggcgaa ttagaagctg cggtcgcaac
    39181 ggacagtcag tttaaaggat tgttccagtg ggagttttac caagattcat atgatgcagc
    39241 ttttatggct ccttctcatg aaccgctatt tgcaaacgat gacgatttca gaatcacaga
    39301 cgacggagct gcaacgcttg atctagatga agatacgaaa aaagcgacaa tcaaagtaaa
    39361 agacaacgtc aaatggtcag atggtgaaga tgtaacggcg gacgatgtga ttttccctta
    39421 cgaaatcatt gggaataaag attacactgg tatccgttat gacgacactt tccgtaacat
    39481 cgtaggtatg gaagaatatc atgatggaag tgccgacaca atctccggaa tcaaaaaaat
    39541 agacgacaaa acagttgaaa tcgaatataa agaagtaaac ccaagcatgc ttcaatcggg
    39601 tggtggtatc tggtcatatg cagcaccaaa acatacgtta gaaggtgtag caatcaaaga
    39661 tatggaatca agcgatcaag tacgtaaaaa tccagtgact tttggccctt attacatgag
    39721 taatatcgtt gcaggagaat cagtagaata cttgccaaac gagcactact ggaatggtac
    39781 gcctaaattg aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa ctgaagcatt
    39841 gaactctaaa ttatatgata tgattttcca aatgccaaca gatacttacg atacctataa
    39901 agaaattgct ggctatacaa acttaggccg tgaacaaact tcttatactt atctaggatt
    39961 caagttaggt aaatggaatg cagatacaaa gagtgtggaa tacaatccag attcaaaaat
    40021 ggctgataaa tcattacgtc aagcaatggg ctatgcatta gataataatg cagtaggtga
    40081 acgtttctat gcaggtttga gaagcaatgc gacttcattg attcctccag tattccagtc
    40141 ataccatgat gatgagatga aaggattcac acaagatcta gacaaagcga aaaaactatt
    40201 agatgatgct ggctacaaag atgtggatga cgatggttta agagaagaca aagatggaaa
    40261 taaactaaca atcaactttg cttcgatgtc cggtggggaa acagcgcaac cattggcaga
    40321 ttactatatc caacaatgga aagaaattgg tttggatgtc caatacacaa caggtcgtct
    40381 gatcgaattc aattctttct atgataaatt agaaaacgat gatcctgaaa ttgatgttta
    40441 ccaagctgct tggaatacag gaactgatcc aaatccaagt ggactatggg gaccaaatgc
    40501 tgcgttcaac tacactcgtt atgaatcaga agaaaataca aaattgatca acgatatcag
    40561 ttcaagcaaa gcatttgatg aagagtacca aaaagaagca tatcaaaaat ggcaacaata
    40621 tgcatttgaa gaagcatttg cgattccaac gttgttccgt aatgaagtat tgccagtcaa
    40681 cgaccgtgtc aaagactggg attggtctta tgatgtacca aacccatggg caaatgtttc
    40741 tgttacagca gattcacgtt cataatcact aaaaatgacc gaaaaagggc gatgtccttt
    40801 ttcggttttt tgatattttt ctgtttattt ctctaatatt caggtcaagg cgctgatttc
    40861 atgacttctc caataaacgg tgttcaaagt ccagtttctc ggtattaagt caaatttatg
    40921 caaaaatatg gaaagccatt ttcacaaaat tttttcttca tactcgaaac taaacgaatt
    40981 tttcacaact tctcgtcaat cactaggatt attaaagctt tacttacctt ttgaccgaac
    41041 catttcaaat ttgacaaaaa accagtataa taaggaagta tcgaaaaaat atccataaaa
    41101 ggaatgtgga aaatggacaa ccagctgaca aaagaactaa aagaaaacta taacatcgtt
    41161 tttcacgatc taaatttgct tgaacaagct tttacccatt catcctatgt gaatgagcat
    41221 cgcaatctgc aattatcgga taatgagcga cttgaatttt taggagatgc cgttttagaa
    41281 ctgatggttt ctcagtattt attccgttta tatcctcatt tgccagaagg aaaattgaca
    41341 aaaacacgag ctgcgatcgt tcgcgaagac agcttgtcga aatttgccaa agaatgtcac
    41401 tttgatcaat atgttttgtt gggcaaggga gaagagaatt caggaggacg tacgcgacca
    41461 gcattgcttt gcgacctatt cgaagctttt ttaggcgcgt tatatcttga ccagggattc
    41521 gacacgaccc atgcattcat cgaaaaagtg atttttccta aagtcaaagc aggtgctttt
    41581 tcacatgaga tggatcataa aacaaaacta caagaagtct tgcaaaaatc aggtgatgta
    41641 tcaatcgaat accgattgat caatgaagaa ggtccagctc atgaacgagt attttggatc
    41701 gaagtttatg tagatgatca attgattggc actggacaag ggaaatcgaa aaaattagca
    41761 gaacaagctg cggcggaaaa tgcattggca gcactgtaaa agtaagtgag ggaaagcttt
    41821 gtatttgaaa cgaattgaaa tagccggttt taaatcattt gcagacaaga cagtcattga
    41881 ttttgaaaat agcgtcacag cagtggttgg accaaatggc agcggaaaaa gcaatattac
    41941 agaggctatc cgctgggtat taggagaaca atctgctaaa agtctgcgag gcggaaaaat
    42001 gccggatatc atctttgccg gttccgatac ccgtaagcag ctgaatattg cagaagtcac
    42061 agtgatttta gacaatagtg atcattatct gccattggag tacaatgaga tcagtgtaac
    42121 ccgccgttat cgacggactg gggaaagcga gtttttcatc aataaacaaa gctgcagatt
    42181 aaaagatatc caagaattgt tcttagattc tggattagga aaagaatcgt tctcaatcat
    42241 ttctcaagga aaagtagaag cgattttcag tagtaaaccg gaagaccgtc gtgggatctt
    42301 tgaagaagca gctggtgtct tgaaatacaa gcagcggaaa aagaaagcag aacagaagct
    42361 ttttgaaaca gaagacaacc tcagccgcgt ccaagacatc atccacgaat tggaagaaca
    42421 gttaactcct ttggccgccc agagtgaggc agcaaaggag tttttacggc taaaagaaac
    42481 actgactcaa accgatgtct ctttgatggt tgctgaaatc aagacagcaa aaaaagattg
    42541 ggacaacaaa caagcacagc ttgcgaaatt caatctagaa ttaggcaaac tatctgagtc
    42601 gatccaagaa caagaatcca ttttggcgaa gcagcgtaaa gaaaatgcgc aagccgatcg
    42661 tctgatcgaa aaaaatcagc aagtattatt agatctttcg gaaaaactga agcagacaga
    42721 aggacaaaaa gatgttttgc aggagcggac aaagcataca caaaaaagct cgcaagaata
    42781 tcaaacttca ttagcagaag cacaaaaaaa ggttaaacat tttgaaaagc ttcaagaaag
    42841 cttgatgaaa gcagcagcag aaaaagaaac agagatccaa aaagctgaag caaatctcat
    42901 aaaaacacag caagaattag aaaaatacca aaaatcaacg aaagaattat tggcagaatt
    42961 aagagatcaa tatgttgatt tgatgcaaga acaagcagct gtaggaaacg aactgaagta
    43021 tctagaacgt caatatatac aagaaacagc aaaaagcaag cagacgttag ccaagcaatc
    43081 ggaagtagaa gcatcagtag accgattgat cttacaaaaa gaagaattga cacaaaagca
    43141 ggcgcagcta aaatcatctt taactgaaac aaaagagaag ttagaaatga tccaacaaaa
    43201 cgggaaaaag ttccaagaag cattagcaaa agaacagcct aaaatgtatc aattaatgaa
    43261 tcaagtccag cagctacgtg ctcgtaagaa aagccttcaa gaaatccaag aaaactattt
    43321 tggtttttac caaggggttc gattggtctt gcagcataaa cagcagcttt cgggtattgt
    43381 cggagcagtg gctgaactga tcgatgttcc tgcagatttc acgttagcta tcgaagcagc
    43441 tttaggtggg gcagcccagc atgtcatcgt tgaaaatgaa aaagatgctc ggcaagccat
    43501 cacgtatttg aaacagcaaa gaggaggaag agcaacattc cttccactga cgacaatcaa
    43561 accaagacaa ttaccggctc atatattgac acaagcagca gctgtagaag ggtttatagg
    43621 gattgccagt gaacaagtca gttatcccga tcaaatccaa actgtggttc aaaacctgct
    43681 aggaacgatc cttttagcca aagacttaac tagtgccaat gcaatcgcgc aaacgatccg
    43741 ctatcaatat cgagtggttt ccttagaagg tgatgtgatg aatgccggag gatccatgac
    43801 aggtggagcg aataaacgag gaaaccaagg cagtttgttt gtacaaaatc aagaattgaa
    43861 acagttgact agtgaatttg aagaagcaga taaacaactc caagcacaag aaaaaaaagt
    43921 tcaggagctg caacaagaaa cagctcgttt agccgaagaa caagaagttt tgcgtacacg
    43981 aggcgaacaa ttacgttttg aagaacaaga agcgacaaat cagctacaaa acatcataaa
    44041 tgaacttgaa cgttttgaaa aagaaaagca aatctcaact tttgagacac gtgaactgca
    44101 gcagtttatc gaagattacg aaaagcaaac gaacgaattg aaagacaaac aaacagatct
    44161 tgaatcgcaa cgacaaaaaa tcgacgaaga aatcaaatcg ttaagtcaag aaagcgacca
    44221 gatggaagcc agaagagcac aagtacaaag tcaaaaagca caagaacaag cagatcttgc
    44281 tgtcttgaaa gagcagttta atcatctgca gatccagctc cgcggtgccc gagtccaaaa
    44341 agcagaggca accgaacgtc aagaagcgat agaaaagcag ttggcgacat tgacagcaga
    44401 cttttctgat catgaagtca cagaagaaag tttggaaaaa caaattaacg aattatctgc
    44461 tcagcgagaa acattaaaag ccgagttggc aaaagccaaa gagcagcgag atagaaggca
    44521 aaaggagatc gataagcttg aagcagtctt ggcagagcgt aatcgtgagc aaaaagcacg
    44581 actgtcggaa caatccaaat tagaagtcca aaaagaccga gctgaaatga ttttagataa
    44641 tcatttgacc tatctgcagt cagaatatca gctgacattt gagaaagctt cccaagacta
    44701 tcaagaaaca accgatatcg aagacagtag aacaaaggtt tcatccttaa aagaacagat
    44761 tgaaaaactg ggaccagtaa acttaaattc aatcgaacaa tacgaacaag tcagtgaaag
    44821 acatacattc ttagcaacac agcgagatga tctgttagca gcgaaaaacc aattgtttga
    44881 aacgatggat gagatggatg atgaagtgcg gacaagattc aaagaagtat ttgaagctat
    44941 ccgtcaagaa ttcaaaattg tctttccaaa tatgtttggc ggcggtaaag ctgaattagt
    45001 actgacggaa ccaactgatt tgctgaaaac aggtattgag attgaagtac aacctcctgg
    45061 caaaaaactg caaagtctaa gtttgctttc tggaggagaa cgagcactta cagccatcgc
    45121 gttgctcttt tccattatcc gtgtctgtcc tgttccgttt tgtatcttag atgaggtaga
    45181 agcagcgtta gatgaagcca acgtgaaacg ttttggtcgt tacttgagcg atttccaaga
    45241 tgacacacaa tttatcgtag tcacacaccg caaaggaacg atggtcgcag cagatgtact
    45301 gtacggagtg acgatgcagg aatccggtgt atcaaaaatc gtttctgtac ggatggaaga
    45361 tatcaatgaa gaggcgaaaa tcaacgaaac gaaagtgtag gaacgaaaat gatcaaatta
    45421 gtagcaatcg atttggacgg tacattatta aatgaacaaa agctgatttc agatgagaat
    45481 aaacaagcat tagcacaagc aaaacagcaa ggggtcaaga tcgttctatg tacaggacgc
    45541 cccttagctg cgatggcgca ttatctgcaa gaattaggac tggtagatga aggtgactac
    45601 agcatcactt tcaacggcgg tttggtacaa aaaaatgata ctggtgaaat catggaaaag
    45661 aaagtgatgg aagtatcaga tatccaacgc ttatacaaac tggctcagca attggatctg
    45721 ccactagatg ttttgtctga caatgtggtt ttacagctgc ctagtgcccc aaggaagcaa
    45781 tccttgtaca atgtgttgaa taagctgctg caattccaac cagcggatat aagcgatatt
    45841 acagatgaat ttgtgcttaa taaggcagta atcgcttatg accaagaaga attagatcct
    45901 aaaatcaaag aaataccagc agaattccat gaaatgtatg agatcatcaa aacaagaagt
    45961 atgcttttgg aatttatgcc aaaaggtgtt acaaaagctt atggtatttc tttattggca
    46021 aaagatcttg gtttgaaaca agaagaaatc atggcgatcg gtgatgagga aaatgattta
    46081 ccgatgatcc aatatgctgg actaggcgta gcgatggaga atgctgtgcc tttcgtcaaa
    46141 gaagcagcag atttcgtgac agccagcaat gaagaaaatg gcgtagccaa agtgatccaa
    46201 aaatttgtat tagagtaggg ggagtcaaat gggatttttt gataaaatca agcgtgcttt
    46261 tactggcgaa gagaaaacag aagaagttga agtacaggag aagtacgaca agggtttaga
    46321 aaagacgcgt aaaacatttg gtgaacggat gaatgagttg tttgccaatt ttcgaacagt
    46381 cgatgaagac ttttttgaag aattagaaga aacacttatc ggtgcagatg taggttttga
    46441 aacagcgata cgaatcaccg aagcattacg tcaagaagtg aaactcagaa atgcgaaaaa
    46501 atcggcagaa gtccaaaatg cgatcatcga gaaacttgtg gatctctatg aagcggaagg
    46561 aatcaatgaa gtaaatgaac taaatatcca agaagacgga ctaacagtta ttttgtttgt
    46621 cggtgttaat ggtgtaggga aaacaacaag tattggtaaa cttgcccatc agttcaaaaa
    46681 cgaaggtaaa aaagtattga tggcagcagc agataccttc cgcgctggag caatcgacca
    46741 gttagttgtc tggggcgaac gcgctggtgt tgaagttgtt cgtggcaatg caggaggcga
    46801 tccagcagct gtcgtattcg atgctttgga aagagcaaag acagagcagg cagatgtttt
    46861 actagttgat actgcaggcc gactgcaaaa taaagtgaac ttgatgaacg aattagaaaa
    46921 aataaaacga gtaatccagc gggaattacc agaagcaccc caagaaacac ttcttgtatt
    46981 agatgcaacc actggtcaaa acgcgatggt tcaagcaaaa caattcaaag aaacaacaga
    47041 tgtgaccggt cttgttttga caaaattaga tggtacagca aagggcggta tcgtacttgc
    47101 tatccgtaat gaattgcatt taccagtgaa attggtcggt cttggtgaag gaatcgatga
    47161 cttggagccg tttgatccaa atgattttgt cgttggattg ttcaaaggat tactaaaaga
    47221 agaataaccc atgtctaaaa agcaggagct gtgacaaaag ttgcagctcc ttgtttgaat
    47281 gattgttttt aacatagctg tgtttttgat gccttcattt ttatacttat ttgtaagcgg
    47341 ttctcaaaca atttcgttct ttttcttaga ctttttgatt ataattagat tcatatgaat
    47401 tggaggtcac ttaatgatga attttaagca attattattg catgtcaatg cgcgcccttt
    47461 tatcgaccaa gctcgatttg gtatagaacg ggaaggacag cgggttgatc ttgcaggaaa
    47521 tctagcaaaa accgatcatc cagcaatctt tggcgatcga tcctatcacc cctatatcca
    47581 aacagatttt agcgaaacac aaacagagat gatcacccct gttaccgatt ctattcccga
    47641 attatttcag tatctcgctg ctgtttatga tgtgactgct cgttctatac cgaaagaaga
    47701 gatgatctgg ccattaagta tgccacctgc cttaccggaa aaagacgaag agatcattat
    47761 tgcaaaatta aaaaatttcg aagatgtctt gtatcgacgt tatttagcaa aagaatacgg
    47821 gaagcgaaaa cagatggtaa gcggtattca tttcaatttt gaatttggcg atgagttgct
    47881 aagaacattg ttcagccatc aggaagaatt tcaagatttt tccgaattca aaacagaact
    47941 ttatttgaaa acagcgagaa actttatgcg ctatcgctgg atgatcactt atttattcgg
    48001 tgcttcacca atgagtgaga agaactactt tttagatgaa tcacatccgc aagaacctgt
    48061 tcgcagtatt cgcaacagtg cgctgggcta tacgaatcat ccaaatgtga aagtttccta
    48121 tgcttctatg aaacagtatt tagcagatat cgagcgaatg atcgaagaag ggaaactttc
    48181 ggaggagaaa gaattttata caccgcttcg tttccgaggc ggaaagaaag tcgcagattt
    48241 agcaacaaca ggtgttcgtt atattgaatt gcggaatatc gatttgaatc cttatgcaag
    48301 attgggtatc aatccagagc aagttcggtt cttacaatta ttcctgatgt acatgttatg
    48361 gacagaagaa aaagaagact gtgaccaatg ggtagcagaa ggtacgactc gaaataacaa
    48421 agtagcacta gaacaaccat cagatcaaac agaatttcat caagaaggta gagaaattct
    48481 tgaaggaatg aagcaaatgc tggttgaatt ggattggttg gattctcttt atttagtaga
    48541 ggaagcattg actcaaatgg atcatcctga acaaacgtta gcagcgaaac tctatcaaga
    48601 agcacagcta tcgagtcagc aagaagttgc tgtggcattg ggacatcaat attataaaga
    48661 aagccatgaa cgtccctatc agttggccgg ttttcgcgaa atggaacttt ctactcagat
    48721 atttatgttc gatgccatcc aaaaaggtgt ccaagtcaaa gtattagatg agtcggatca
    48781 atttttgcgt ctgcaattcc aagaccatgt agaatatgtg aaaaatgcaa acatgacaag
    48841 taaggacagc tatatcgtcc cacttatcat ggaaaacaaa acagtaacga aaaaagtctt
    48901 aaaagaagct gggttccggg taccaggagg cgcagaattt tcatccatgg aagaagcggt
    48961 aaaagcttat ccgagatttg ccgagcaggc atttgtgatc aaacctaaat caaccaatta
    49021 cgggttaggt atcacgattt tcaaggaagg agctagtctg gaagattatc aagcaggttt
    49081 agccattgct tttcgtgaag acagttcggt tttagtagaa gaatttatgc cgggaacgga
    49141 atatcgtttc tttgtgatcg acggagaagt ccaagccatc atgttgcgag tccctgctaa
    49201 tgttattggt gacagtatcc gaacagtgaa agaacttgtt gaagaaaaaa atagtgaccc
    49261 tttgcgtgga acgaatcatc gtgcaccttt agaattgatc caattaggtg aattagaaca
    49321 attgatgttg aaagaacaag ggttgacgat cgaatcagtt ccccaagcca atcaaattgt
    49381 ttatctaagg gagaactcaa atatcagtac aggtggtgat tcgatcgata tgactgatga
    49441 attttctgaa gcttacaaaa aaatcgccgt ttctgcggta gaagcactag gagctaagat
    49501 tagtggtatc gatttgatta taccagataa agaaatcgat cctacaactg ataaaaaagc
    49561 atatgggatc atcgaagcaa atttcaatcc agccatgcat atgcatgttt atccttttgc
    49621 aggaaaaggc agaagattga cgatgaatgt attgaaattg ttatatcctg aagtatttta
    49681 aaagaaaaag cttttgacag attgtcctat ttgatgtcaa tctgatcaaa agcttttttt
    49741 gcatgtaaaa atttatgtgt tttttcgttt taaaataaac gatcccagaa gctttttttc
    49801 ttttttggtt tttctttttc agctggcgaa gagttttctt ctgggtattt tttttcgata
    49861 tcaatcacag gttcatttac tgcttctttt gctgttaaga gcaatccgaa ttcctcagga
    49921 tttgcagtga cgtaatcgtt gactacagtg aaacggctat tgtttttcgt tgctagctgg
    49981 atatattggt tttggacaga aaggggtaga gccccattca agagtatgga agcatctgga
    50041 tgttttttga gttcttttaa aaagttttct ttgttttttg gttggatcat ttcagcaaca
    50101 gtcatactaa gatagcagcg ttccctgaat gtccctaaat atttatgctg ttcttcaggg
    50161 ttcactaaag gtgtaccata aagtccctta tccaaatggc tttttacatc atcttgtgcc
    50221 ataaaaaata ccctccttct tctctttgtc tttcttacga ttatagcata ttcccaataa
    50281 tacctaaaac atatgaaaaa gcggttgctt ttgttcaacg attcacttaa aataagaatg
    50341 aaaatcattc tcaattagaa aggtggattg gatgcgctac ggacgactag tagcattatt
    50401 agttatttta agtagtattt cattgttcat cggagtatcg aatgtacaat tgtcagaact
    50461 gctccagtta gatgggcaaa gcttacaaat cttgctagtg actcgtttgc cacgaacgat
    50521 ttgtttgatc cttgtgggag cgaccagtag tatttgtggg ttgatcatgc aacatttgac
    50581 ccaaaataaa tttgtttcac caacgactgc gggcacgatg gatagtgcgc gtttaggtat
    50641 tttagtggct atgattttcc tacctggtgc atccttgatt gtccgatcct tgacagcatt
    50701 ttgctttgct tttgcaggaa cattgctttt tttatccctt acgcgattgt tcctccagaa
    50761 aaatcaattg atgcttccgt tgattggagt catgttaggg aatatcattg gttcagttgc
    50821 tacttttttt gcttatcaat tccagttggt acaaaatatg acttcatggc ttcaagggaa
    50881 ttttgcgact gtgatacgag gaaattatga gttattgtat gctgttgttc cattgttgct
    50941 cttggtttgg ctatttgctt accgatttac gatcattggt ctgggcgaat cgtttgcagt
    51001 aaatctggga atagattacc agaagatgca gtttttaggc attggcttag tatcattggc
    51061 tagtgcagtc atgttgatca tggtaggaag cattcctttt atcggagtga tcattccgaa
    51121 tcttgtctct agaatatatg gagatcaagt gcacaaaaca attggcgtaa ctgctttagc
    51181 aggtagtttg ttcttagtat tttgcgatct tgtcgctcgc gtgttgattt ttccttatga
    51241 aataccagtc agtgtgatcg ttggaatcat cggtggggct atcttccttt atcttctgat
    51301 aaggggacga cgatatgctt aaaaaaaagc catctatttt tattggtatg cttgttcttg
    51361 ttttatttgt tacaggaatg tacttgacga tccatacata tggaaactgg cattttgcat
    51421 gtgcattgag gagtaagaaa atttttgcat ttatccttgt ctcgctagct gtcagcagtg
    51481 ccacaatcag ctttcagaca attacgcata accagttttt gactccttca gtattgggac
    51541 ttgagtcgtt atatgtcctt gtccaaacgg tccttttctt ctttatcgga ggagtgacga
    51601 tgcttcagca gacaggtaca gtttttttct tattgaacgt tgggctgatg gtcgggctga
    51661 gtctattgtt atttttcttt cttttgaaaa aaatgagagc gaatcttttt ttattactga
    51721 tgtcagggat gattttaggt acgctttttg gtagtgtcag taccttttta caagtcatca
    51781 tggatccgaa tgaatatgat ttgcttcaag gcagattatt tgcaagcttt ggcaatatcc
    51841 agagtcagta tcttttgcct gcttctttga tcattatcgg ctgctggtac ctgttatttc
    51901 gatatcgaca tgtgctagac gttttccatc tcggaaatga acaagcagta aacctcggga
    51961 tagacacaga tcgttttgaa tttttgattt tatgttgtgt cagtcttttg acagggacat
    52021 caacggctct tgtgggtcca gttacttttt tagggtttat tgtcgcaaat atcagttatc
    52081 gtgtcttttc tacttatcgt catggtcagc tatttatcgg aagctttctg gtagctatgc
    52141 tgtttctgac aggaggacag ctcgcagtag agcagatttt cggctggaat acaacagtga
    52201 gtgtcgtcgt tgaatttgtc ggaggaattt attttgtcac aaaattgatt cgcgaaagga
    52261 agagtatcct atgattgatt tacaaaaggt caataaactg taccatggaa aatcagtggt
    52321 taaagatgta cagttaacga ttcctgaaaa gaagttgact gcttttatcg gtccaaatgg
    52381 tgctggaaag tcaacggtgt tgtcgatgat cagtcgtttg ataccaaaag atacaggtga
    52441 aatctattta gaccataacg aagtaaaggc ttggaaatcc aatgaattag ccaaaaaatt
    52501 atcgatccta cgacaatcga atgaaataaa tgtccaaatc accgtgaaag aattagtaga
    52561 atttggacga tttccttata cgaaaggaaa attgacgaca aaagacgagg aaatcgttca
    52621 ggaatcactg gaacacattg ggttagaaga aatagcggat caatcgattg acactctttc
    52681 aggcggtcaa ctccaacgtg cgtatattgc aatggtgcta gcgcaagaca cggattatat
    52741 tttactggat gaacctttga acaatctaga tatgaatttt gcggttcaaa tcatgcagat
    52801 tttaaaaaat ttagtacaag aaatcgggaa aacaattgtt attgttcttc atgatatcaa
    52861 ctttgctgca agttatgcag acgaaattgt tgcaatgaaa gatggacagt tatttacgca
    52921 aggaacgacc caagaaatga taaaaaaaga tgtattggat gaattatacg aaatggacat
    52981 acggatttgt gagattgaag gccgcagatt ctgtctttat ttctaaaact aacattagga
    53041 gagaagagat gaaaaaatta gtagtaggta tcttgattct agcatcattt ggtttagcag
    53101 catgtggaaa tagcaacaac accagtcagg cagacacaaa aaaaagcagc acacaaactg
    53161 aaactacatt gacgataaca gatagtaatg gagaccaaat cgaggtgcca aataatccag
    53221 aaaaagtcgt cgtattcgac aatggttctt tagatacgat ggatgctctt ggtgttggag
    53281 ataaggtagt tggagcagct acaagcagcc ttcctgagta tctttcatcc tataaaaaag
    53341 tagaatcagc aggtggaata aaagaaccag acttagaaaa aattaatcag cttcagccag
    53401 atctaatcat catttctggc cgacaaagag attttcaaaa agatctaagt caaattgctc
    53461 cgaccatttt cctttcttta gatgcgaaaa atccatggga atctttccag caaaatgtca
    53521 ctgctttagg ggagatcttt ggtaagcaag aagaagctaa aactcaatta gaggaattat
    53581 ctagtgcaat cgatcagacg aaaaagaaag cagaagctac tgacaaaaaa gcactagtca
    53641 cacttgtgaa tgaagggcaa ttatcagcct acggatctgg ttctagattc ggtttcatcc
    53701 atgatttatt tggctttgaa caagcagatg atcaaatcga agcatccact cacggtcaaa
    53761 gtgtttctta tgaatatgtg ctggaaaaaa atccggatat cttatttgta gtagacagaa
    53821 caaaagctat tggtggagac gacagcaaag atgatatttc tgccaatgaa ctggtagcac
    53881 aaacgaatgc tggtaaaaat cagcagatca tctcccttga accagatgtc tggtatttaa
    53941 gcgggggcgg tttagaatca atgaaactga tgatcgaaga tgtgaatcaa gcatttaaat
    54001 agcataaata acagaggatt aaactctcat actatgtaac aataatgttt cttacattct
    54061 taaatagcaa gagcctatcc tcaaagaagt tatcaaaaac catccgtaaa atcgtattga
    54121 tgattttacg gatggttttt atttatttga gaaatctttt ttcaacgtga attcattatc
    54181 tacatcatac gggttgattc tagaatgggg agtggcaaaa aatagtgtga tccatttttt
    54241 cagcatcaag atcgcctcct ttgatttgaa tcaaaggtac aaagcgaaac ttagataaaa
    54301 cttaaattta gatattttta ttttgtaccg atctcttctt taaacgatgt tccatctgct
    54361 gcttctcggc aataagtcca ctagactaga aaatatgaga agctattttc tgtccagcgt
    54421 tcttattact caaagcaaat cgcagttgtc acaatctctt ctaaacaatg ttcaatcagc
    54481 tgaccttcgg tattagctca aagcactgat ttcacaacct ctatttaggc atgtgtctca
    54541 gcaagcggtt gatagttgat gatctgcatt tttcgataaa aatcggcggg aagcttactc
    54601 caccgtttta gtaattgata gaaataactc tcttctgctt tgattttata ctcgcttaat
    54661 atcaatccaa aactattcgt tttttttgaa gctaattttt tataaatatc caatgctttg
    54721 gttttatctt cattgacaaa gagtgttttt acttcaatat aagaaccctc gatattttgt
    54781 gtttcttcga taaaatagat gttttttata tattccttca tagtgattcc tccaaagaag
    54841 tcttttattt gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta
    54901 attctttttc caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa
    54961 gaaaggaata aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa
    55021 ctgaacgaag aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat
    55081 gacttacttt ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga
    55141 ataaagtgat tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag
    55201 cacaagaagt attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa
    55261 acggtgacgg tccatttgca gaaagccaag tcagttgcca attggatacg gcatttcgat
    55321 tgatcattgt caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata
    55381 caccagttgg accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca
    55441 cacttgcttt agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa
    55501 aaggcaactt gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca
    55561 tcagcgacct acatgtggac atcaataaat tcggcgaatc agagctgatg cagctttggc
    55621 aagttttgca aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag
    55681 tagaccgttg cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc
    55741 atttcggcaa tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag
    55801 acccacattt tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga
    55861 tgaatggctg gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa
    55921 tgaagcgttt gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca
    55981 atgcagaaat gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg
    56041 aggtgatttt atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc
    56101 cttatgagcg ttggaacaaa ttaaatgcat ttttaggatc agaatcattc ggagaattac
    56161 ttgaccgtta ttcaaatatc aaacaagtag tttttggcca tacgcatcgt cgctttgaag
    56221 ataaatccat cggtcacacg atttacagct gcagaccttt tggttattat tatgaatggc
    56281 agctgacccg agattttgtc ttgaaagaac agttgctaga cagctatgat cctatgaaat
    56341 tacgtgtcct cttgcgtgcc catcacccag cttttcaggc ctatcaaaca caacatttgc
    56401 aagaggaatt tgaacaagcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt
    56461 aaaagaaaat accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg
    56521 atatgctgct ttttttgcgg aaaataatca gcgaatgttt atttgatcgg atgatttgta
    56581 actcgcagag tactagatgc aagcgtgtat tctttttttg aacgataatc acaaaaaaaa
    56641 atataatata cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag
    56701 tcgagagtgt aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aag
//
LOCUS       DACEDO010000009        55201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEDO010000009 DACEDO010000000
VERSION     DACEDO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.009"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..931)
                     /locus_tag="IWO52_000605"
     CDS             complement(<1..931)
                     /locus_tag="IWO52_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354895.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTE"
     gene            complement(1186..1695)
                     /gene="mreD"
                     /locus_tag="IWO52_000606"
     CDS             complement(1186..1695)
                     /gene="mreD"
                     /locus_tag="IWO52_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0354896.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1699..2553)
                     /gene="mreC"
                     /locus_tag="IWO52_000607"
     CDS             complement(1699..2553)
                     /gene="mreC"
                     /locus_tag="IWO52_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0354897.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2708..3346)
                     /locus_tag="IWO52_000608"
     CDS             complement(2708..3346)
                     /locus_tag="IWO52_000608"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0354898.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3347..3997)
                     /locus_tag="IWO52_000609"
     CDS             complement(3347..3997)
                     /locus_tag="IWO52_000609"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0354899.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4010..4909)
                     /gene="rsgA"
                     /locus_tag="IWO52_000610"
     CDS             complement(4010..4909)
                     /gene="rsgA"
                     /locus_tag="IWO52_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0354900.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5074..7143)
                     /gene="pknB"
                     /locus_tag="IWO52_000611"
     CDS             complement(5074..7143)
                     /gene="pknB"
                     /locus_tag="IWO52_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0354901.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7140..7880)
                     /locus_tag="IWO52_000612"
     CDS             complement(7140..7880)
                     /locus_tag="IWO52_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0354902.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7893..9251)
                     /gene="rsmB"
                     /locus_tag="IWO52_000613"
     CDS             complement(7893..9251)
                     /gene="rsmB"
                     /locus_tag="IWO52_000613"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0354903.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9251..10189)
                     /locus_tag="IWO52_000614"
     CDS             complement(9251..10189)
                     /locus_tag="IWO52_000614"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0354904.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10182..10673)
                     /gene="def"
                     /locus_tag="IWO52_000615"
     CDS             complement(10182..10673)
                     /gene="def"
                     /locus_tag="IWO52_000615"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0354905.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10688..13096)
                     /gene="priA"
                     /locus_tag="IWO52_000616"
     CDS             complement(10688..13096)
                     /gene="priA"
                     /locus_tag="IWO52_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0354906.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13349..13654)
                     /locus_tag="IWO52_000617"
     CDS             complement(13349..13654)
                     /locus_tag="IWO52_000617"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0354907.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13659..14273)
                     /gene="gmk"
                     /locus_tag="IWO52_000618"
     CDS             complement(13659..14273)
                     /gene="gmk"
                     /locus_tag="IWO52_000618"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0354908.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14760..15044)
                     /locus_tag="IWO52_000619"
     CDS             complement(14760..15044)
                     /locus_tag="IWO52_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354909.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15062..15952)
                     /locus_tag="IWO52_000620"
     CDS             complement(15062..15952)
                     /locus_tag="IWO52_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0354910.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(15972..16190)
                     /locus_tag="IWO52_000621"
     CDS             complement(15972..16190)
                     /locus_tag="IWO52_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354911.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16160..17860)
                     /locus_tag="IWO52_000622"
     CDS             complement(16160..17860)
                     /locus_tag="IWO52_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0354912.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18127..18900
                     /locus_tag="IWO52_000623"
     CDS             18127..18900
                     /locus_tag="IWO52_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354913.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(18981..19550)
                     /gene="thiT"
                     /locus_tag="IWO52_000624"
     CDS             complement(18981..19550)
                     /gene="thiT"
                     /locus_tag="IWO52_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0354914.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19616..19718)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19724..20695)
                     /locus_tag="IWO52_000625"
     CDS             complement(19724..20695)
                     /locus_tag="IWO52_000625"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0354915.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20789..22162)
                     /gene="glmU"
                     /locus_tag="IWO52_000626"
     CDS             complement(20789..22162)
                     /gene="glmU"
                     /locus_tag="IWO52_000626"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0354916.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22665..23483)
                     /gene="purR"
                     /locus_tag="IWO52_000627"
     CDS             complement(22665..23483)
                     /gene="purR"
                     /locus_tag="IWO52_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0354917.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23650..24474)
                     /locus_tag="IWO52_000628"
     CDS             complement(23650..24474)
                     /locus_tag="IWO52_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0354918.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24447..25127)
                     /locus_tag="IWO52_000629"
     CDS             complement(24447..25127)
                     /locus_tag="IWO52_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354919.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25150..26088)
                     /locus_tag="IWO52_000630"
     CDS             complement(25150..26088)
                     /locus_tag="IWO52_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0354920.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26333..27184)
                     /locus_tag="IWO52_000631"
     CDS             complement(26333..27184)
                     /locus_tag="IWO52_000631"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0354921.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27338..29911)
                     /gene="mprF"
                     /locus_tag="IWO52_000632"
     CDS             complement(27338..29911)
                     /gene="mprF"
                     /locus_tag="IWO52_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0354922.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30052..30858
                     /gene="proC"
                     /locus_tag="IWO52_000633"
     CDS             30052..30858
                     /gene="proC"
                     /locus_tag="IWO52_000633"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0354923.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30937..32493)
                     /gene="rny"
                     /locus_tag="IWO52_000634"
     CDS             complement(30937..32493)
                     /gene="rny"
                     /locus_tag="IWO52_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0354924.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32904..33953)
                     /gene="recA"
                     /locus_tag="IWO52_000635"
     CDS             complement(32904..33953)
                     /gene="recA"
                     /locus_tag="IWO52_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0354925.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34062..35303)
                     /locus_tag="IWO52_000636"
     CDS             complement(34062..35303)
                     /locus_tag="IWO52_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0354926.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35543..36121)
                     /gene="pgsA"
                     /locus_tag="IWO52_000637"
     CDS             complement(35543..36121)
                     /gene="pgsA"
                     /locus_tag="IWO52_000637"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0354927.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36330..37214)
                     /locus_tag="IWO52_000638"
     CDS             complement(36330..37214)
                     /locus_tag="IWO52_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0354928.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(37992..39278)
                     /locus_tag="IWO52_000639"
     CDS             complement(37992..39278)
                     /locus_tag="IWO52_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0354929.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39271..40539)
                     /locus_tag="IWO52_000640"
     CDS             complement(39271..40539)
                     /locus_tag="IWO52_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0354930.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40791..41252
                     /gene="mscL"
                     /locus_tag="IWO52_000641"
     CDS             40791..41252
                     /gene="mscL"
                     /locus_tag="IWO52_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0354931.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41311..41757)
                     /locus_tag="IWO52_000642"
     CDS             complement(41311..41757)
                     /locus_tag="IWO52_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0354932.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41850..42095)
                     /locus_tag="IWO52_000643"
     CDS             complement(41850..42095)
                     /locus_tag="IWO52_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354933.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42210..42788)
                     /locus_tag="IWO52_000644"
     CDS             complement(42210..42788)
                     /locus_tag="IWO52_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0354934.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42865..43395)
                     /locus_tag="IWO52_000645"
     CDS             complement(42865..43395)
                     /locus_tag="IWO52_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0354935.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43399..44259)
                     /gene="rlmB"
                     /locus_tag="IWO52_000646"
     CDS             complement(43399..44259)
                     /gene="rlmB"
                     /locus_tag="IWO52_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0354936.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44252..44647)
                     /locus_tag="IWO52_000647"
     CDS             complement(44252..44647)
                     /locus_tag="IWO52_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0354937.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44644..46056)
                     /locus_tag="IWO52_000648"
     CDS             complement(44644..46056)
                     /locus_tag="IWO52_000648"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0354938.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46053..46589)
                     /gene="cysE"
                     /locus_tag="IWO52_000649"
     CDS             complement(46053..46589)
                     /gene="cysE"
                     /locus_tag="IWO52_000649"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0354939.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(46911..48371)
                     /locus_tag="IWO52_000650"
     CDS             complement(46911..48371)
                     /locus_tag="IWO52_000650"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0354940.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPKMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48541..49698)
                     /locus_tag="IWO52_000651"
     CDS             complement(48541..49698)
                     /locus_tag="IWO52_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0354941.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49732..51102)
                     /gene="radA"
                     /locus_tag="IWO52_000652"
     CDS             complement(49732..51102)
                     /gene="radA"
                     /locus_tag="IWO52_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0354942.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51165..51692)
                     /locus_tag="IWO52_000653"
     CDS             complement(51165..51692)
                     /locus_tag="IWO52_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0354943.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51922..52728
                     /gene="proB"
                     /locus_tag="IWO52_000654"
     CDS             51922..52728
                     /gene="proB"
                     /locus_tag="IWO52_000654"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0354944.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52725..53972
                     /locus_tag="IWO52_000655"
     CDS             52725..53972
                     /locus_tag="IWO52_000655"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0354945.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54048..54392
                     /locus_tag="IWO52_000656"
     CDS             54048..54392
                     /locus_tag="IWO52_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354946.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54509..54835)
                     /locus_tag="IWO52_000657"
     CDS             complement(54509..54835)
                     /locus_tag="IWO52_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354947.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT        15152 a        11956 c         9898 g        18195 t
ORIGIN      
        1 cttctgttga agattcaggt gttgttgatt cttctgttgc tgaagattgt gtagctgatg
       61 atgattcttc agtcattgaa gattgtgtag ctgatgattc ttctgttgct gagctttgtg
      121 cagtagatga agcttgagta gcttgtgctt cagcctctgc ttgttcgcgt gcttcttttt
      181 cagctttttc ttgtgcagct tgttgacgag cttgttctgc tagacgttgt tgttcacgaa
      241 tacgagcttg ctctgcttca gcttctgctt tttgacggtt caagtcagct ttcttgtctt
      301 cagcagttgc ttgttcagca gctaatgaag ttttcagtac gttcaaatca gcttgtttag
      361 ataaaagatc gcctttttga gattccaaag cagcttggtt ttcagctaat tcttttagtt
      421 tagcatcgtt ttcagctttt ttgtcttcta cagctttttt atcttgtttt tgttgttcca
      481 tcaagtcgtt gtttgctttt accatcgttg tcattgcttg aacgcgtcca atagcatctg
      541 ctaatgaatc agcattcaat acagcatcga tgtagtttga gcttgtgttg cttacttgtg
      601 cttcgcgtgc ttgtttttgg attgtatctt cacgtttttc aatacgttct tgtaaatctg
      661 caatgtcttt tactaattgt gcagattctt gacgcaatgt atcttgtttt gctaataggt
      721 cttgtgcttg tgtgttgatt tcagaaactt ggctttctaa ggcatcgatt tgtgattgtg
      781 catctgcttg ttgatttttc aagtctgcaa tcttttgatc ttgctgttgt atctgagaat
      841 caaaatcgtc cgccgcggca gcgatcggtg atgctacggc agtcaaggtc attgaacata
      901 ccattactgc tgatattaaa ctctttttca ctcttcattc ctccgactgg cttaatttaa
      961 taaatatcta aatatattta agtctttctt aacgatataa aaattgtacc atagcagtgt
     1021 gtcaacgata taatagttat gttacaaaaa tgtttcaaaa caacttttaa aaatgacaaa
     1081 actgttcgtt tcagaaaatg atcatgaaaa agccgcgtta agtctagcat ttcttaagat
     1141 aacaacgatt ctttttcgct ttcgacctaa aaataaaaaa agaaattatt catcactgaa
     1201 taatttctta aagggaaaaa tgaataatac aaatataatc atgttcaaca gcagtgtagg
     1261 tcctaaaaac cttgtaataa aatacgtgtt atttactact gctaatttaa aaatcaactg
     1321 gaccaccatc gtaaacaatt cataacccgt gacaaagatg atcatactga aaaattcagt
     1381 aaagatgttg acatggataa cgtctttcat tacatacatc aaccatacaa ttaaagggag
     1441 agctactgca tagataccga taacgccaat atagtaagcg tcatagatag ccccgagaac
     1501 aatcgtggta atcagtaaat aacgtttttc aaacgccata ctgcaacata ataatgccaa
     1561 tatcagaaag tgggcatttg acatataggt gcctttcgac cactccccta gcattcttgt
     1621 taaatgacca tctatcaaca tcaaaaagaa caaaacgatt ggcagatagt acttcattgt
     1681 ttctttcttc aacatggact aatcctccgc tagccgttgg acaatcgtca caactgacac
     1741 gtcatacatt tctgcataag gtttcacata aacttcccga tccagcccat aactatctgg
     1801 tttcgtttta ataaccgtac cgatcggcaa gttagctgga gaattccctc caagaccgga
     1861 tgtctggaca acatcccctt ctttgatatc cgtatcacca gttaattggg tcaccactaa
     1921 ggcatggagc ttttcatcat agtttttaag caaaccaaac gcttcgccat tagccgaaga
     1981 tacccgtact ggaaaatgat tggagctttc attagaggat gacagtaatt cgattttaga
     2041 cgaagccgta ttgacctcga tcactcggcc aatcaatcct ttttgcgaca tgactgccat
     2101 atttgcttcg ataccgtctt tagaaccctt gtctacgatc agcatgtcct gccaactgtc
     2161 aggcgaacga gtaatgacat ttgccgttac tttttcgtaa ctagtcagcg tctgattcaa
     2221 ctcaagttct tttttcaacg cttcgatttc tctctcgtaa ttcttgtttt gctgtgaaac
     2281 gtcatcatag ctatcgattt tttcttttag ccgctcattt tcggcataag ttgaaaaaag
     2341 attatggaca gaatctactc catttcgtac ccaccgtact ggggcagaga ttgttttatc
     2401 aaccattgca acactgtcat taacgatgga ttggaagaaa ttgggctttc cttcattcgc
     2461 tcgctgcgca gcggtcacac ttagtatcgt caccacgatg ataacaataa ccaaggtaat
     2521 aataatattt ttattaggat tgaacttctt cacaaagaca cctcgaatta attagactct
     2581 tgtgttcaat tttatcacta aatgccgtga aacaaaaggt aagttgctcc tcttaagctt
     2641 ttttttagca aataatagat tctgtcattt ttagcctgaa agacaaattt ttttatttgt
     2701 ttatggatca atctttgctt tgaataactg caatcatacc atctgaaaaa gaaaattctg
     2761 cggtatctga aagaaattcg ttacttgcat aagatgtcgg atattctact tctttatttt
     2821 ccaatacata cttgctgcct tttaatgtaa gattcgagac gggagtcaag cagcaataag
     2881 ctaaatactt catatctggt atctgtctta ctatatgctc tccaggcaaa taataagaca
     2941 aataattttg acaatctctg ataataagct gatgggcaaa gggtttgaag cgaggttcca
     3001 tacctaacca gagattggct aaaagatgat ccaatcttcc gcccgtagct ccaatcaaag
     3061 tgacttccgc ttgcgggtaa cgttcaaatg tttttaagat acctaactgt gtatctgtat
     3121 cgtctttttc tgcaggggaa cgggtatact ctttggcttt tgcaaatact tccttctgct
     3181 cttcgttcga caatgaatca aaatcgccca ctgccagatc tactgggtag cctgcttgaa
     3241 gcaggtacag cgcaccacgg tctatcccga cataggcatc gtatttggac agcagagact
     3301 gcggccagtt ttgcggatta cctccagcag ctaataaaat tttcatttag tctaatgcct
     3361 cttttaatcg ttcgatttgt ccgactggat tttcggctcc atagatatat gatcctgcaa
     3421 cgaagacatc cgcccctgca tctttacaga tttttgctgt ttctggaacg attccgccat
     3481 ctacttcgat tgcataagtc caattgttct ctctacgtag atcagcgagt tccgtaattt
     3541 tttctactgt ttcttcaata aagctttgtc cgccgaaccc aggattgaca gtcatgacta
     3601 gtacttgatc agccaaaggc aaaacatgtt tgatcatgga gactggtgtt cctggattga
     3661 taactactcc agctttaact cctaagcttt ttgtcatttg aagtgcccga tgaatatgag
     3721 gagttgcttc gacgtggaca gtaatgatat ctgctccagc ttttgcaaat gctggaatgt
     3781 agttttctgg atcaacgatc atcagatgga cgtctaacgg taatttagta acaggacgga
     3841 ttgcctgaac aatatttggc cctaatgtga tatttgggac aaattggcca tccattacat
     3901 ctacgtggat gtaatctgcg cctccttttt ccactaactc aatgtctctt tgtaaattgg
     3961 caaaatctgc actcaaaatg gatggtgcta tcttcattct tgtttcctcc tattttcgtt
     4021 taccttcttt ttttgcatac attggttttc ttttttcgat ttcttgtaag aattgcaaat
     4081 aattgtcgta acgtgtctgt gcgataccac ctgtttctac tctatgtttt acttcgcagc
     4141 caggttcttt agcatgcatg cactctctaa atttgcaatg gacagaagct tctacaaatt
     4201 caggaaattg tctcggcagt tctcctgctt caattgtcaa aaagtcaatc gagctaaatc
     4261 ctggtgtatc tgctactaac ccgccataga taggaatcag ttccacatga cgtgttgtat
     4321 gccgtcctct tccaagtgca tcagaaatct ctcctgttgc taaattcagt tccggtgata
     4381 tttgattcag tagcgtcgat tttcctgctc cagactgacc cataaagacc gatagccgtt
     4441 ctgggaagta acggatcagt tctgtcgtat cacccgtatg attgggtaca atcaccggat
     4501 agcctatttt agtataggtt tgcttgatct cttcgatttt ttctgggtcg tctgacaagt
     4561 cggtctttgt caaaaaaata atcggttcga tcgactcgta ttccaaggta actagaaagc
     4621 ggtcaagtag attataagaa aatgcaggct ctacgatact tgtgacaatg attccaagat
     4681 caacatttgt gactggtggg cgaactaatt gattttttct cggcaagatt tccagaagat
     4741 agccgtctgt ttgattttca ctctcaaaga tcacctgatc tccgacaagc ggcgtgattt
     4801 tccgattccg gaaatttcct cttcctcttg tttgataggt ctctccgttt gcgtatacat
     4861 agtaaaaacc gctgattgct tttctgattt gtcctttgat aagtgccacc tccgtttttg
     4921 tttattttac catacgaaga ggaaagtagg tgttattcca tgcaaacttc tcaaagtata
     4981 tggcttgaaa attgatcaaa agagatagaa acacaaaaaa gagaatgcaa caaatcaatg
     5041 tcgcattccc ttctgatata ttttattcag cacttatttt gaactttctg aggtgctagc
     5101 tgatgaactt gtggagctgg aagattcttc ttttgaagag ttgcttgtgc tactggagct
     5161 acttgagctg ctagaactgt ctgaagagtc tttttcagaa ctagaagacg ctggatctgg
     5221 tcctttagag taaattacgt tcacaaccat tcctttggta attgtcgttc ctgcccctgg
     5281 tactgtacga atcactttat ctttatccac cgtgtttgaa tattcggaag tctcatgccc
     5341 gatatattct gcaccgacac tagctaaata actttgtgct tcgctttttg tatagccgga
     5401 gatatcattc aatttaggct ctgttccttg gcttacgaca aaattgatgt aagtttcttc
     5461 tgctacaact tcacccgagg ctggatcctg actgatgatc gaacctgatg gtacagtgtc
     5521 gctatagtca taagtagcac taatattatt gatattgaat ccttgtgtaa caagccaatt
     5581 acgaacagtt tcgtagtttt ctcctacatg actactcaaa gtcactgttt ctggtccttt
     5641 gctgactgtg aaagtaatcg tgtctgtttt aggatcgact tctgtatctt ctgatggaga
     5701 ttgcgaaatg atcattccgc tttctacctc tgaactgaat tcctcttctt tttgaatcaa
     5761 gtcagcagaa aaccctaatt ctttcaaact tgcttcggca tcttcataac ttttaccact
     5821 gtaatcatct aatttgattt ttttcgtgcc gctactaata tacagagtga cttcgcgttt
     5881 ttgcttgact gtcgttcccg ctgccgggtc tgtcttcact acattacctt cttcgatttt
     5941 gtcatctgca atttcttctg tttttgttgc tacttctaat ttcgcttttg cgagtgtttc
     6001 tctagcagaa gcttcagaaa gatcagaaac atcaggaaca acaacttctc ctcgcccgcc
     6061 agaagcaaag aatacagctg ttccgattcc taaaacagca agaatagcta ataggatcag
     6121 ccaccatttt ttctgtcctt tctttttagg atcaggcaga acttcttcct cctctggtaa
     6181 ggtttcgttt tctggcttgt cttttggcag aggcatagat ttgaatgaat caggcattgg
     6241 tgcatcttcc gtaaggggtg tgataacttt tgtttcatta tccatcacat gaggctgcca
     6301 tttttcttcg tttgcacgag ctggtgataa tactgtagaa agatcatcag acatttcctc
     6361 ggctgactta taacggtctg caggttcttt agccgttgcc cgtaaaatca cgttttccag
     6421 tgcttgtggc acatttggat caagtgcttt tatagatgga agatcatctt gaaaatgttt
     6481 taaagcaatt gttacagcag attcaccatc aaacggtacg ctacccgtca acatctcata
     6541 aaggatgatc cctaaagcat aaatatctga ctgcttcgtt gccatacttc ctctggcctg
     6601 ctcaggggac agataatgaa cagatcctag catcgtattc gtctgcgtga tcgatgtttc
     6661 agataaagcg atagcaatcc cgaaatccgt gatttttaca acaccttcat tatcgatcag
     6721 tacgttttgg ggtttcaaat ctcggtggat gatttgatgg ttatgagcta aagaaatagc
     6781 agaaaggatc tgctgcataa tatttactgc tgtttcataa ggaacaggat aatgtgtttg
     6841 gatatatcgt ttcaagtcca ttcctttgac gtattccatc accagatact gcatgttgtc
     6901 ttcttcacca acatcgtaaa cactaacaat gtttggatga acgagttctg ttgctgctaa
     6961 agcttctctc tgaaatcgtc gaatcgcagt ctggtcattt tgaaagtcaa atcgtaagac
     7021 tttaactgct acatcacgat ccaagattaa atcgtgcgcc aaaaatacat ttgccatacc
     7081 accgctaccg atatttccag tgatttgata acggccattt aattttttgc ccaactcgat
     7141 catacactgt cctccttata ttcgataaga agaacggtga tattatccgc tccgcctgct
     7201 tcattggcac gatcaatcag ctcagttact ttatgagaca atgggccttc ttgattgatg
     7261 atcgttccta tttcttcttc agacagcatg ttcgtcaagc catctgaaca taacagcagt
     7321 cgatctttta gttgccaaag atgagtagaa acatctactt cgactgttcc aggcatgccg
     7381 acagaacgtg ttaaaatatt tttccttgga tggttcactg ccatttcttc gctgatttct
     7441 cctgatttga ctagttcatt gactaaagaa tgatcctccg tcaattgcat gattttatct
     7501 tcacgaatca aataagctct gctatcccca acattggcaa tcgtgaacca gtcatctgac
     7561 agagcggccg ctacgattgt tgttcccata ccgttatatt ccggctgttc ttgtccacgc
     7621 tgataaatca gcatattttc ttcttgaatc gattgaatga accattgggc gattttttca
     7681 tcgtcaacag cttcttgttt ttcccaagct tcgcctagat tcgttaccgc catctgactg
     7741 gcaatgtctc cggcacgatg accacccatc ccgtctgcta atacagcaag cttgagacct
     7801 gaacggctgg taaatacact ggcatagtct tgatttgtgt tacgtcgtct tcctacatct
     7861 gattgatatt caatctgcat tttttcacct cattactttt tgcgcaaaca acaaatgaaa
     7921 aatccatctg tgtaaaactg atgcggatac aaagtcaaca ttttctcttc gattgatgat
     7981 tgtagcatag gattggtcaa aacatcaatc ctctcaaaat cagggtgtct ttccaagaaa
     8041 gcagaaacaa cgtcttggtt ttcttctttt aagatcgtac aagtgctata agtcagaata
     8101 ccggaagact ttaaagttgg tgcgcagctt tctaaaattt ccaattggat ttttggtaaa
     8161 ttcgctaatt catccggctg ttttttataa cgaatatctg gttttcgccg aagcagccca
     8221 agacctgaac aaggcgcgtc tactaaaatc cgatcaaact gctctgcttc gaactgttta
     8281 ctgacttctc tagcatccat cttttgagcg tagacagcag aagagacacc tagacgtgct
     8341 gcattatctt caatgagttt gactttgtgt tcgtgtacat ctaaagcgac cacttctcca
     8401 ccgcatgacg gatctaaaaa agaagcaata tgcgtcgtct tacctccggg tgctgcacag
     8461 gcatctaaga catgatgttc aggctcgatt tgcatagacg gcgctacaag catcgaactt
     8521 tcatcttgga tcgtcatgac gccatcgtgg aacaatgagc tagaagctaa atgccctttt
     8581 ttcgctacta taccgtatgg actgacttgg cttttttcag ctgtgatctc ttcttcttgc
     8641 aaaacagtga tggcctgatc tctggaaagt cggcgtgtat ctactcgggc actagcatgg
     8701 ctaggctgat atagcgataa accgagcttt tcagtttctt cgtatccgat ttgatccacc
     8761 agtcgtttcg tcatccatag aggcaggctg attttgacag aaagtctttc gataggatca
     8821 gcaatgcctt gcagatctgg tgctccttga cgctgataat tcctcaagac accatttaca
     8881 aatttccctg tcccaggatt tccttttact tttgcgattt cgaccgcctc gtttaaaata
     8941 gcatgtgcag ggactttatc taaaaattct aattggtata cagaaagata aagaagcatc
     9001 ttgacccagt catccacttt ttttgcttta gcgatcaatg gtgcaacgta aaattccaat
     9061 agtcgctgat ggctaatcgt accatagatc aattctgtaa ataaacccac atctttttga
     9121 tttagctcgc tttgtttgat catctcattc aacaaaagat tcgagtaggc gccacccttg
     9181 ttcactcgct ctaatgtcag taatgccata tatcgtactg attttttcat ttttttaggg
     9241 atttttttca ttatttcacc tgctgaccaa tctgaacttg ctgaccgctt ccgttaagga
     9301 aatcatgtac ggcctgtttt cctttaccag ctggctgtaa ttcattgatg gctaagacgg
     9361 tctgtttacc gcaagcaatc catagatttt ttttgtcttt ccggatgatc gtaccaggtg
     9421 ctttgtctgt agtttctgcc aatacttcaa ccgataataa tttccagcgt atgccctcat
     9481 aggtcgtgaa tgcaattggc caaggacgca ttccgcgtac ttggttgtca atttcttctg
     9541 cagttttatg ccaatcgatt gcttcttgtt cccgtgtgat gtttggcgaa aaagttgctt
     9601 ctgactcgtc ttgaggtact ggtttttgtc catccaaaat attgggcaat gtttctagca
     9661 atagctttct tcctaaaagg ctaagctttt caaacatcgt ccctacatca tcttgtttag
     9721 tgattggtat gctttcttgc gcatagattc cgcctgcatc catttttttg atcatttcca
     9781 tgatcgttac accagtttct ttctccccat tcatgatggc ataatgaaca ggcgcacctc
     9841 cccgatattt aggaagtaaa gaagcatgga cattgattgc ccctaatttt ggtacttgca
     9901 ataacttttc aggcaagaat tggccaaatg ccgcagtgac gatcacatca ggcaccaatt
     9961 caatgatttc ttccatttct ggcgaaccgg agattttctc tggctgcagc acacggatac
    10021 cgtgcttcag agccgctttt tttaccggtg tcggcgtaat tactcgtttc cgcccaactg
    10081 gacggtcggg ctgtgttacc actgcctgga tctcgtaacc tgattctatc agcccttcaa
    10141 gaatcggcac agaaaaagca ggtgtgccca taaatactag cttagtcatc aagatgttcc
    10201 tccatatatt cttcaagttc ttcttccggg atctgctgta tcattttctc aatgaacaag
    10261 ataccgtcta agtgatcgat ttcatgctgg aaggcacggg ctaaatagcc aaaagccgtc
    10321 acttctatct cttctccgtc tcgatcataa taacgcacgg tcacttcatc tgctcgcttg
    10381 accgttccat agacatgcgg aatactgaga caaccttcta cgtcaaggct ttcacctttt
    10441 gcttcgatga tttctgggtt aatcagttca aatttctcgc cttcatctac ttcgatcaca
    10501 gcaattctct tgttttgtcc tacttgagga gcagcgatcc ctattccgtc attcgcaatc
    10561 attgtctcat ataaattgtc taataaagcg attgtctcgt ccgtgattac atcgattggc
    10621 tgagcagttc tttttaattt atcattaggg tggatcaata taggatagcg cataatttgg
    10681 ccctttctta aataaagttt aatggttcgt tgtcaattgc cacaaaaaga ccttgtcgct
    10741 gttctttctg gcttccattc aaaatttcat ccaaaacatg aggaagattc ggttcatgtt
    10801 tatatttgat gattagttga taataatatc ggtttttcac tctcaaaatc atgctgggcg
    10861 ttggccctaa tagcaagctt tgagggctta acacttcttt tagctgattg gcaatctgaa
    10921 agattttctt tgccgctact tgttcttcag gatggctggc ggttattttc acagtaaagt
    10981 aataaggagg atagcctcca cgatgccgta catgcatttc ctgctgataa aactgctcat
    11041 aattttgttt tttagccaat tctatggcgt aatgttgagg attgaatgtt tggatcacga
    11101 cttcgcctgc tttttccgct cgccctgctc gtcctgaaac ttgggtaagc aattggaatg
    11161 tatgctcact agaacgaaaa tccggtaaat tcagggaggt atcagcattc aacaccccaa
    11221 ctaatgtcac ttctggaaag tccaaacctt tagcaatcat ttgtgtccct aataatatat
    11281 ctgcttcttt tgcaccgaat ttttgtaaaa tctgttcgtg tgcccctttt cttcgagtgg
    11341 tatctacatc catacgcaaa atcctagctt gcggataaag ttctcggagt tcctcttcta
    11401 ctttttgagt acctgttcca tagtagcgga ttttattgcc gccacaatta ggacagcgat
    11461 tgggaatagg ttcttcatgt ccgcaataat ggcagcgcat tgaacgtgta tccatatgca
    11521 gtgtaagaga gatatcgcaa ttaggacatg gaagaacaaa tccacagtcc ctacacatca
    11581 caaaagacga atatcctcga cgattgagca gtaggacagt ttgttctttt ttgtctaatc
    11641 gattttgtat tttttcctgc aaattagcag aaaaagtaga ggtccgatga ttctgaaact
    11701 cttcacgcat atctacgatc tcaatagctg gaagctgtgc agctgctttg gctcggtggt
    11761 ttaactgcaa taactgataa acgccttttt gagctcgtgc tcttgattct aaagagggcg
    11821 tcgcgcttcc tagaactatt gggcaatgat gatatttact gcgccaaatt gctagatctc
    11881 tggcatgata acgcggtgtc tcatcttgtt tgtaacttga ttcatgttct tcatcgataa
    11941 tgatcagccc gatatttttc agcggggcaa aaatagcaga tcgtgcgcct actactactt
    12001 gcgcttcttc ccgctcaatc ttgcgccatt catcatattt ttcaccttgg gaaagtccgc
    12061 tatgtaagac cgcaactgat tctccgaagc gtcctttgaa tcgctcgacc atttgcggag
    12121 tcaatgcgat ttcaggcact aacataatag ccgttttatt ttctgacaaa acatcagcga
    12181 ttgcctgaag atatacttct gttttcccgc tgccagtgat tccctccagc aaaaagacct
    12241 gatctttttg ctgctgacct gctgtgatga tttgttcgac agcatttttt tgttctgcat
    12301 tcagttccaa tgccgtcgtt tggtcaaata ctcggtcttt ataagggtcg cgataacgtt
    12361 ctgattctat aaactccagc cagccctttt cttttccttg attcaagaca gcagtactga
    12421 ttcctttatt tttgaaataa gcaattggtt cttcttggct cagttcagat aaacacaaaa
    12481 gaagttcttt ttgtttcttg gcattttgcc gtaattttgc ccattcttct tcgatttgct
    12541 tttcagttaa caaagaacgg accaaacgaa tcgtttttac tttatttcgt gtatggactt
    12601 catatttgat atctactaat tgctcttggc gccagcgaat caattgagaa aggctgcctt
    12661 cttcttctgc ttccttccag gaaatcactt ctcgctgatc aaacaatacc tgaacttctg
    12721 gtgtgtctgc caaaggatag atcaatttat catattctgc tttcatcaca ctcggaagca
    12781 tcgtttgtag acaagtgatt ttgaatgcgt aagtaatttt tttcatataa tcagctaatg
    12841 aaagtaattc ttcattgacc accggagcaa ggtctagcaa acgaataact gccttcaaag
    12901 atggattagc tgattcttct gatttttgga tagccataac aaatccttgg acatgacgat
    12961 tgccattacc aaaagggact tctactcgca tcccgacttg caaagctgtt tccatttcag
    13021 agggaaccaa ataagtgaat ggctgatcgg tctgcatagt aggtacgtcc acaatgactt
    13081 cagctgttaa tgtcatggtt cgtttcctcc tttttttgtc ttttcttatc aagcgacaaa
    13141 aagttccttg cctattttac tagagatcga tggatttgtc ttatcttctg ccaaatccta
    13201 acaaaaaatt aacagcagat aagaaaaaaa agaactgtgt caaaaagttc agacacagcc
    13261 ttttctacca gacaataagt ggcgaatgtt tgaaaaggaa acagctgggt ttgcttttct
    13321 ttttagatgt tcgctctatt tttctttatt acattttttg ctcttcacgg atacgtgatt
    13381 ccaattcgtg ttgttcacgc tctttttgtg cttgacgttc ctcttcttcc atcttcaaac
    13441 gttcacgttt taattcagga tgtggatcat tgactacatt tcctgcatcg atttcttcta
    13501 aagcttgacc gacgcttttt actgaatcaa aagaatctag agtaggctgt gcacttgcat
    13561 ccaattcatg cgcgcgttta cttgctaaaa tcactagaga atattttgaa tttacacgat
    13621 ctaatagtga gtcaattgaa ggttttaaca tcataagact acatctcctt taacatttta
    13681 atatatttac cgattacgcg atctactcga aaatgttcac tggcaataat gtttttgatt
    13741 cgttcaactg ctagcggcac ttcatcattt acaacagcat agtcgtaaag agccatcatt
    13801 tcgatttctt cttttgcaac tttcattcgt ttttcgatta cttcatctgc atctgttcct
    13861 cggcctacga tccgtgattt taattccgct aaatctggcg gagtcaaaaa gatgaataca
    13921 ccatcgggca ctttttcttt gacttgtttt gctccttgga cttcaatctc caagaaaaca
    13981 tcttttcctt catccaatgt cttgttgaca tatgacaaag gagttccata ataattcccg
    14041 acatattctg cgtattccag catctctcca gcttcgatca ttgattcaaa ctcttctttg
    14101 cttcggaaga aataatcaac gccttcgacc tccccttcac gcattttacg ggtagtcatt
    14161 gagatcgaat actgaaaatc attatcctca ctctcaaaaa ttgcttttcg tactgtgcct
    14221 tttcctacac cggatggacc agacaacacg attaataatc cacgctctga catgacgacg
    14281 tcctttcaac tagtcttttt gtacttatcg tttattttgc actttttttc tgcaactttc
    14341 aaggttatct ttaattatag ctagaaattg gtaaaaaagc taccctgtac caagaggtcg
    14401 taaaaaagct gtcctgcatc aagtaataag aacagccaaa cataaacagc ttctcatgtt
    14461 tttcgtttgg ttggggttat tatcgcagat gcaagctttt gaacgccgtg tataagaggt
    14521 cgtgaagaag ccgattagtt tcgagtatga agaaaaattt tgtgaaaata gtttctcaca
    14581 tttttgcata aatttggctt aataccgaga aaatggtttt tgaacatcac ttattcaaaa
    14641 taaaaaaaga ttgtgacacg aattttgtca caacctttaa atgtattcct agagcaaaga
    14701 agctaagcga agaaatccaa ttctgtttat ttattgtatt tctttgttca gcatccttgc
    14761 taaaagccca tattggacaa agtattcact acacccatga tctcatcttt tcgagaacgt
    14821 gctttttctg tcggatcttc tacctcttct gtgtcaattc gaacaataaa tttccgattc
    14881 atcgtcacgt aagcaccgtc gttgatcaaa ataaacagtt cgttctcatt atagtctgca
    14941 caggcatctt tccatacgtc aaagccgttt ttttcagaag ggatatcccg tttaaccaca
    15001 gtttttccac ttaattcgtg gaacgttacg tcataatatt tcattatctc tacctctttc
    15061 gtcattcaat attttgtact tgttctctga ttttttctaa gatcgtctta gactggacga
    15121 cttgattttt aatttcgatt gcatttgatt tggaaccgat cgtgttgatt tcccgattca
    15181 tttcttggat caggaaatct aattcccgac ctacaggaca ttgtgcttcg ataagttcat
    15241 ctagtttttg tacatggatc gccagacgat ccagttcctc gtgaatatct ccgcgttcca
    15301 acaagatggc tagctccgtc aacaaccgtt gctcatcgac tttcgctcca agaaattcct
    15361 ccagcttttt ttgatacctt atttgaaatt ccttttcata gatttctaca aaactatcga
    15421 gtttttttag tacttctttg aattcttcag tattcttttt caacactaaa gctaaagctt
    15481 gaccttcttt ttctcgactc gcctcaatag aatcaagtgc gcttttcaat gtatctagag
    15541 ctaactgtgc caattcttcc atgctctctt ccggtttttc ttctaccgtt acgaagtttt
    15601 cgtccagcac gaagcgttcc gccatccgac taattgacac ttgattttga ctaccgtatc
    15661 gttgttgaat tccgtctgtc aattgagtga taagtgaatc aatcaattcc cagttgatga
    15721 cgatctgttt ttcgtttttg ccaatataag ctagattcac aaatacttcg attcgaccgc
    15781 ggcttaactg ctgtttaata agctgacgaa aatcgttttc aacaaagttc aggattttag
    15841 gacttcgtat ttgaacatct agaaaacgct ggttgacact ttttatttca atatctaact
    15901 gatacgcttt tgtttcgcgg atggctttgc caaaaccagt catacttttc attcgacagg
    15961 gtccttcctc attacatatt ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc
    16021 aatgtcaggc ctttccccat tttttcatgg tctggcgccc aatctcgaag atcgaatttt
    16081 ggcgggcgac cattccagct gactaggttc agttctttac gccacccctt gttgttttct
    16141 gacaatatag cgatttcttc tacgatttca tatgaaaatt cttgtgccat ggttgttcct
    16201 ccttgagata atataaatat aggatcagta atgtgtcaac tggttgctta agcaattcat
    16261 gctgcaaacg atccttgcgt tttccttgta gcagataatc aaaataaaca gttttcgtgc
    16321 gctcattttg tattttttgc tggtattttc gataccttcg ccattcttca gtacttaatt
    16381 gaccaatttg tgccaataaa tcatccggca tactttttat atcttgttgg cagatcattt
    16441 gaatcatcat gtctacttga tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa
    16501 gttctagcat cgccaatccg catgtctctg gtacgaccct gttcttatat aaattacgca
    16561 gtttttgcca aaattttatg cttttgaaaa aagataattc taactgaatt tcttttgtta
    16621 agtaagacaa aaatcctcgc cagtatgttc ttgcaggttt aacagaatga aatgtatacc
    16681 agttcccata gtaatagcct aatcgttttt tcatttccag atagcctaat tgtaaattta
    16741 attggttcct ttggctatca aaaagcagaa agctgcctag tgtccacaat gtttgacatt
    16801 tccaatggat aaaaatgtct ggcatacgaa tttttttcgc tgggcctggc ccttgcacat
    16861 ctacgacaaa tgcctctgtt gctcctttgt taatcgcaat atcaataggt attttgttgc
    16921 gatacccacc gtctgcatat ttattttttc cgatctttcg ataagccata gcgggataaa
    16981 atgaagcaga ggcgagtatc caatctggta tttcttctgt ttttgtctgt tggatatcag
    17041 tcactacttc cgctagttct ggcagtttag tagacaccgt atataaacgt attttgggat
    17101 tatgtttgag taaggacaaa tccaaattgt ctttgaccat cttttccaat ggagcagaag
    17161 aaaccccgcc ttcaattagt gctgttttcg tcatttggcg tctttctcga tagaagcgtt
    17221 cacgaaggtc ttcaactgca gccatttctg gtaacgcaag cacttgtctt gtcgacagct
    17281 ttttccataa attcacagct tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg
    17341 cacctaccga ggtacctgtg ataatctcaa agttgatatc atgttccttc agcgcctgcc
    17401 acacaccaat ctgataagca ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat
    17461 agaccagctt tttatttaat tgtgtggttc cttgatagcc gtttttatga aaaatacgca
    17521 accattcttc tgatagagga gattgaaata aaaaacaaca gttttcttta ataaaaaaac
    17581 gagcgcaagc ttccatatac cgaaaaaatt ttttccattc aaaagtttca ccttcaatca
    17641 acagattatt aaaaataaca tctcctctac ttttagcaat agtaaaatag acggtatatt
    17701 ttttcgatac tgctttaaaa gcacattgct tttttgcaga acgccaaaaa gcgtctctac
    17761 tttcttgatg actttgaaaa aatggaaggg gttgttttgc ttgctggata ggcgaataca
    17821 gcgacatttt ctgtgaaaag taaacttttt ttatctccat cgcgctctcc tttttcttca
    17881 agtataccag tacgtcataa aaaggtcatg agtctcgtat tcgactttgt agattccata
    17941 agaaaacaat tcataaaacc tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt
    18001 gaaaatgaga gtgttttcaa tgaaaacgtg attttgtaat ggaattttaa aataaaagaa
    18061 acaagctttt ttaatgcaaa tgttgtaaaa taaaagagat taagaggaaa taagaggagg
    18121 aacatgatga ctgagtcagt ttatgtacat attgatacca caagtaatgc tgttttaaca
    18181 aaagggctga ctgctactga ctttgcaaac agtatcgtgc attttcctca aaacttgctc
    18241 ttgcttgatc catctgcttc tgctggagaa tatgaatccc atacagggct gaaagtaatc
    18301 cgtggtacag aaaatattca gcgtttcttt tcctcttctc gtaacaggcg tggattcgat
    18361 gaattgaagt ggatcgactt tactgattta acgatgctga aagaattgac acctttagaa
    18421 atttcagaat tattatattt tggtcatatg aaaacacatc ttcattcgcc gtttttttat
    18481 aaactacaga ataattttgt atacttcgat cttaatgatc agctgaatcg gatttattat
    18541 cgctatttag atgaatttta taggattttt gctagtaaat taagttcgat cttgtcagat
    18601 aagatcaatg taaaaaaatc ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg
    18661 gaattattga aagaaatgcg taatttgatg caggaaggga tcattttttc ttttgatcaa
    18721 gttggtttga tcgatggtaa atatcgtatc ccaatctatg tgattgaaga ttatatttgg
    18781 aaattaaaag gcacccggta tcgagaagag gaaatcatcg ctactttaat ctactctgtc
    18841 accgatcgac actggcaatt ggtagaagat gccgatcttt cttatctgcc attgaagtag
    18901 atctatttct tatcaaaaaa taaaaaggaa acaagaaact aggacatgat tctgtctcaa
    18961 gtttcttgct tcttttttat ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga
    19021 gtcgtttgtt ggatccctaa taaaacgatg atcgttacaa tagctgtact caacccgctg
    19081 gcaccattca ttacgaaaga aaagagccaa ggattcatcc cccatagtgc atacgctccc
    19141 cagaaaacca ctcctgcaat aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca
    19201 ccaaacacga tgctttttct aatttgtttc gtattatcgt ctacatagcc tttttggaca
    19261 tttgaagcca tgagccctgc tgcgcctgca aaagtatagg caatcgggta ctcaatcaac
    19321 acttgaacga cacttaaata ataaacttgt cccaacggaa aatgtaggat gccccaaatg
    19381 aaacctgcca gcatccctgc ctttgtccct cttcttaacg caaaaagagt caatggaatc
    19441 tgccctaatg aaatagaaaa actgctgccg atctggatcg gaatcaacga caacaccatc
    19501 gctaacgctg caacaatcgt tccttctacc caaactctat tttttcccat aaaaaaactt
    19561 cctcctttga ataaaaaagt cacaaaggag gaagcagctt tcgaaaatag tcaaacagct
    19621 gtttattcac aattcctacg ctcgcataac cgatacaggt acgaagggtt tagaaataga
    19681 ttcaatctca gcccaattgg actccccttt gtgagtttat ttattattta ttttttgttt
    19741 caaacaatgg actaactggt ttgttttcat ggatacgttt gattgcatct cccattagtc
    19801 cgccaacact tacttcatcg attttgtcga ttttacgatc ttctggaaga tagatcgagt
    19861 ctgtaacaac tagacgctcg atagctgaat cttctattct ttgtaaagca gggccggata
    19921 atactggatg tgtacatgaa gcatacacac tagttgcacc agcttctttc aaagcattgg
    19981 cagccaatgt gatcgtacct gctgtatcga tcatatcatc gatcagtaca caagttttgc
    20041 ctttgacttg tccaatgata ttcattactt ctgcaacatt tgcttttgga cggcgtttgt
    20101 ctatgatcgc aatcggagat ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac
    20161 catggtctgg tgaaacaacg acaacatcat cgcctttgat tcctttttct aagaaataat
    20221 tggcaataag tggcgcaccc atcaaatgat cgactggaat atcaaagaaa ccttggattt
    20281 ggactgcatg aaggtccaaa gtcagcattc ttgttgcacc agctttttga atcatgtttg
    20341 ctactaattt cgctgtgatt ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc
    20401 cataataagg catgactacg ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca
    20461 tgatcagcaa ctccatcaag ttatcattga ctgggctgct cgttgattga ataacataga
    20521 catgcgatcc acgaatactt tcttcaatgt ttacttgaat ttctccatca ctaaattgag
    20581 taacagatga ttttcctaac tcgacgccga ctgctgctgc aattttctct gctaatgggc
    20641 gattggaatt taacgcaaaa atttttaatc ttgggtcaaa gtaatgtttt gacatgggga
    20701 cctccacttt ctttttcaca actctagaat tctacctaat aaatagtagt catatttttt
    20761 aaataaatca agtgattttg caaaagtctt agtttagata agggagtttt tttgcatatc
    20821 cttctttatt gacttgtctt gctctcgcaa ttgccagcgc atattctgga atattgtctg
    20881 taattgttga cccggcagca atcgaactgt ttttagctac ctcgacaggt ccgatgatat
    20941 tcgtgctgga accaataaag ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat
    21001 catagttgac gaagacaaca ccacatccga cattgatttc ttctcccagc gtagcatcac
    21061 caacataagt caagtgaccg actttagttc ctttgccgat ttgagcattt ttcacttcca
    21121 caaagtttcc gatatggacg ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag
    21181 gtcccacatc tgcatgtttc ttgacatggc tgctttcaat gactgagttt tcaatgacga
    21241 cgtgatcctc aatcctactg tctacgattt ttgaatgtga cccgatgaca caatcagaac
    21301 caatgactgt atttccttgg atttgtacac ctgcttcgat caatgtgtct ggaccgattt
    21361 ctacgcctgc atcgatgtat gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat
    21421 gcatctggtt aattcgcttt ctcatgatct catttgcttt agctaacgcg atccgatcat
    21481 tgactcccat tgattcttca aaatcatccg tttgataagc agcaactgta tggccttctt
    21541 cttttaagat ttcgatgata tctgttagat agtattcacc ttgagcattg tttgttccaa
    21601 ctttgcttaa cgcatcgaac aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt
    21661 cttgtacaag agcttcttct ggtgttgcat ctttttgttc cacgattttt tctacgatcc
    21721 caatatggtc acggatgatt cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga
    21781 tcgttgcact tgcatttttt ccttggtgat attcaaaaag attgttcagc gtttcagtag
    21841 tcaataatgg tgtatcgcca ctgataacta acgttgttcc tttttttcct tccaagaaag
    21901 aagcagcttg caaaacagca tgtcctgttc ccaattgttc tgcttgtaac gcatatttac
    21961 tgcgttctcc tagttgtgct ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt
    22021 catctggttt cgtttcactg acccgattaa tgatatgttc taccataggt ttaccagaaa
    22081 cgggatgtaa aactttatat aattttgact tcatgcgggt acctttccct gccgccaaaa
    22141 taattgcata acgtgcgtcc aactgcgtca ctcctaaata tagtttgctg atttcagttt
    22201 atcccagtat acccattttt tcaacaaaac aagtgatttt cttttatccc agcgttttct
    22261 ggataaaaaa atagatagac cataacgggc ctatctattt ggtaaccggt gctctcctgc
    22321 caactcctcg gtcacaagtc acactactca taaaacatga aaagctgttt tttgaaatcg
    22381 tttcttgtga cttagagtta aacggtaggc ttacaacctc ttcattaaac gtttttcaat
    22441 cagctgacct tcggtattag ctcaataaac ggcaaaatat gaggagcaat ttttccgttt
    22501 attgagctaa tactcaggac aggacgctga tttcacaacc tctagataaa cggtgttcaa
    22561 aagccagttt ctcggtatta agccaaattt atgaaaaaat gtggaaagcc attttcacaa
    22621 aatttttctt actactcgaa actaagcggc tttttcacaa cctcttattc tgaaaaataa
    22681 tttcctggca caaccgtaat atttttcgtt tgagtatcta catctttgac ataaagcagt
    22741 gacgtataat catcgatggc gcgatgaccg ttgaattttg attcggcaaa tacagtgatg
    22801 ccaacaagtt ctgattcgaa ttcttcaatc aagctcttca ttccgttgac tgttccgccg
    22861 cctttcatga aatcatccac tacaaggaca cgtgaaccac gtttcaaact tctcttagac
    22921 agttccatct tttcaattct ttcagaagat ccagacacat agttgacact tactgttgat
    22981 ccttctgtta tttttgagtc ccggcgtaca ataacgaatg gcgcattcag ataataggac
    23041 acagcttggg caataggtac gccttttgtg gctacagtca ttacagcatc gattttttca
    23101 ccaaggtatt tagaagcaat gattcttcca acttgacgca gcagttcagg ttctcctaac
    23161 aaatcagaga gataaacata tcctcctgga agcagacggt cttgttctga tagacgttct
    23221 gctagagatt caatatattt tttcgcctct tcatatgaaa tttcagggat gaacaataca
    23281 ccacctgcag cacccgggat tgtttccaag gttccatacc ctcgttcttt aaatgttttc
    23341 ttgatgattc ccaaatcttc actgatagaa gatttagcag attcataacg ttcggcaaag
    23401 cttgttaagg aaatcaattc atgcgggtga ttcaataaat actgtgtcat gtcaactaga
    23461 cgttcactgc gacgtacttt cacctattcc aactcctttt aagaaatttt atcgttttac
    23521 attatataag tttttcttca aaaattcgag cattttatga aaaaataaga aaatagttcg
    23581 gtatttatta aaataaaaag aagcctataa aaaatttttc gtttttataa ggcttctttt
    23641 ctttataatt tagttatttt ttcttctgaa ggcaaccaat attcgtttga tgatatttac
    23701 aatcaggaac aaagcaataa agactaatgt gattgttgct cctggaggtg tatctagata
    23761 aaacgagcta gttagtcctg acagcatccc tagaaatcct acacaaacac tgatagcaat
    23821 cacgacattg aagcttttgc ctaatcgcat accgattgct gcgggaagaa ccatgattgc
    23881 ggatatcaaa agtgcaccgg caatcgggat catcacagca atagccacac cagtaatcac
    23941 attgaacatc atcgacatca aatgaacggg tagcccatcc acgtgggcgg tatcttcatc
    24001 aaaagtcaga acatacatcg gccgtttgaa taaaaagaac aatataccga tggcaacgaa
    24061 taagattcct aaaaagatga cttgctccca tgtaatggtc acgatcgaac caaacagata
    24121 ggattggata cttgttgctg aattccctcc gctcaaattc atcaatacta acgctagcgc
    24181 caaaccacca gccattaaaa tggcaatgga gatttctgaa taggttcggt agatcgttcg
    24241 cagatactcc aatatcacag ctgctaatac aacgactagc atggtcgtaa gtgtaggatt
    24301 aaggttcaaa aagaatccta acgctacccc tgctaaagag acatgagaaa gcgtatcagc
    24361 catcagcgat tgtcttctga tcactaaaaa cacacctagc atcggggcga ttccggcaat
    24421 gaagatagcg gcaagaaacg ctcttctcat gaattcatat gaaagcaacg ccattgagaa
    24481 tcctcctttc tcaccagtcg gatctgtctg tccgcatact gtttgatatc ttcatgatca
    24541 tgggtgatca ttagaactga tttcccatgg acatgcgcac tatgtcggag aagacgataa
    24601 aattcatttc tggattcttc atccatacct gttgttggtt catcaagaac aaataagtca
    24661 gggtcagtag caaaaatacg cgctaaactg attcgctgct tttgtccgcc agacaattca
    24721 cctattcgcc gatttcgcat atcccacatc cctacagctt ctaaagcacg ctgtacatgt
    24781 tgatgatctt tttcagagag acgcttgaac cagcggcctc ttggatatct tcctgaacgg
    24841 actaattcga tcaccgtact aggaaaacct gcattgaaag atgcaacttg ttgaggaata
    24901 taaccgatac ttaatttttt tccttcttga tttttctcag caatctttat cattcctttt
    24961 gaaggtttca gtaatcctaa cgatgctttc accaaggttg acttggctgc tccattttct
    25021 ccagttaaaa tgacaaattc acccgcatct acatgataag agacatcttc tagtacaggt
    25081 tcatcatcat agtaaaaggt caaatcagat atttcgatat aacgcacctt acaacctcca
    25141 ttctaaaact taattgatac tttcttttaa agctttcaga tttttttcca tgacagaaat
    25201 ataattttcg cctgctttga tttgttcatt agtcaagctt tctaaaggat tcaacacttc
    25261 tagcttcact cctgtttcat tcgacaatgt ctcagctacc ttcgaagagg cattttcttc
    25321 aaaataaatc actttgatgt tgtttttctt cacaaattct tttagctcag ctaagcgact
    25381 tggtgtgggt tcctgatctg gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg
    25441 attcgctaaa taagcaaaag cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt
    25501 tagctcatcg gagtattttt gatccagtgc ttcaagcttt ttgatataag catcggcatt
    25561 cttcgtaaaa gtttcctttt tatctggata tttttcgcct aattcttcag caatcgttct
    25621 gacttctttt atagctaata ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg
    25681 accatgccct tcttctgatt cttcatgttc gtctgcttct tgcgattcta aacggtcgat
    25741 tcctttagct gcttcgatca tcaaggtctg tttgctatcc actgaatcct ttaacgaatc
    25801 cacaaaaaat tccatgtctg aactattata cacgaaaaca tctgcatcgg aaatttctgc
    25861 tctttctttt gcacttggtt cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt
    25921 cacgtttcct tcatccccta cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt
    25981 aacgatcgtc aaatcctctt ttttgtctgc ttccttgttc gtatttccac atgctgcaaa
    26041 cagtaaagca cctatcatta ccgtagctgt caaaagatat tttttcattt atatgctcct
    26101 ttcaatgaac aaatcggaat tttacgattt attaacgtga actaatgtat caaaataaaa
    26161 aaacacagtc aatagaaatc catcaatttc tgatttaagc atgtatattc ttgattaaat
    26221 cgtaaaatgg atgagttgct gctcatatct agccatttca ctacaataaa aaactgaaga
    26281 ataccatttt aggattcctc agtttttcat cacctatttt acttcttata ttttattgca
    26341 gggttcgtac taaataaact tcctcacaaa aaccttttaa cccattatat actctctgcg
    26401 ctcttgaata atgttgacac aaggcaaaaa cggttggtcc gcttccactc atcaatgcag
    26461 catctgctcc gtacttgatc atacgctctt tgatttgatg gatgactgga tgccgctttc
    26521 ccgttatatc ttctaaactg tttcccattt ttttgatcat ctgctgataa tcttgctctc
    26581 ggatggcttc agcaagtccg tggatgtctt gatgttggat ggcttgcgta tctacttctc
    26641 taaacactgt acgtgtagag acactcattc ttggtttgac caacacaacc cagcactgtg
    26701 gcatagaagg taatacttgg atttcttccc ctttgccacc gacaaatgcc gtacttccgt
    26761 agacacagta aggaacatct gtaccgactt ccaaaccgat ttctgccagt tcttctaacg
    26821 aacagcccag attccataat tggttgatcc ctcttagagc agctgcacag tctgtacttc
    26881 ctccgcctaa tccagctgct actggaatat tcttcttaac aaagatcttg acaccggttt
    26941 tgatattgta tttatttttt agcaataaag cagcttgata aacattgttc cgttcatcta
    27001 ccggtaaaaa tgctttattt gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa
    27061 atagatgatc tgccaagtct acgctcgaca tcaccatttc caactcatga tacccatctt
    27121 gccgtctata taatacatcc agtcctaaat ttatcttggc tggcgctttt tcaatcaatt
    27181 ccatcctctc acccattcga aaagtctaag tcgcaagtat tctatcatag ggaaaaataa
    27241 aagtatagct tatgtctgcc tagtggttga ttttgtcatc gtttcaacag ctttcacaga
    27301 attttctctt cataatccaa acaagttcta gtgattctta taattctgtg agcacttctt
    27361 ctgccaaact cggaacattt cctttttctt ttcctatcag actgagtagc tgcatggtag
    27421 caaatccgaa attacttcct ttcctataag caaaataacg tccttcccaa tgatcgacat
    27481 attttaattt cttttcataa gcagactgga aggaatagaa aaaattacca taattatata
    27541 cgatatgcat gactttttct tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg
    27601 gagctaagcc tagattggct tccgagtagc ccattttctc gtatacaata aacagattgg
    27661 ctaacagata gtcagctaat tcctcagatt ctttaggtaa gacccgcaac aaatcatatc
    27721 ccgcctgctt cttcgtcatc ggctgagcgg tgatgaagcc aacgactttt tgatctttac
    27781 ggacaatacc gacaccgcta gataatacat atgatttatc aaatcgtccc acagagaagt
    27841 atttttctcg tgcaccttga agccattttt gtgaaacttc atttacagct tgatgtaatt
    27901 cttccggcag ttgatcgtac caagtaaaat ggtatccttc tttcatcaaa cgttgatatt
    27961 caagttgctg actcagccga gacaagtctt ctgttgctag atcaacaatc cctgcttcac
    28021 ctactttcgt gaagtgaaaa cccaattcat gaagcatcac tacgtagctt tctgatatct
    28081 tataaaacgc aagctgatag cctaaacaat ctgcttgttt cataaactga ttcgttgctg
    28141 cccgcatcat tttaggattg ccaactggat ctcctaaaac aaagcatttg tttcctttga
    28201 tctgataacc aaaaacgact tgatcttggt cgtcttcttg gtaataaaaa taactgtaac
    28261 ctgacaagcg aagctgatgg cttgtagctg tgcctccgta tgtttccaat aaacggtcaa
    28321 aacggtcttt ttcgtactca ctgccaagct tcgtagtatc ttcagctaaa tattgataaa
    28381 gcgccactaa agctagcaat gagatgccca aaccaataag ccctgagaac cacacatcat
    28441 ctgaaggaaa caaaataaaa tgatttcctg ttattcgacc gactaaaaag cgtccgctgt
    28501 gatagcctgc aattgcgtaa acgataaaca aaaagccgaa caagctagca tcaaagatta
    28561 tagctcccca agaataaacg aattttgttc gataaaattc ttttctggat aaccacacag
    28621 ctaacagaat caagacatat accaatatca actgccaaga taacgtacgt gtgattgtat
    28681 ttaaaatacc aaaagatagt aaagcaatcg taggcagaaa tgcctttttg acttttactg
    28741 aaagtgctct agcaaggccg agtaataaaa agccaaccaa tagattcact gtctgatcta
    28801 aaaaattgaa tgagaacggc aataatactt gaaatagccg actgattgca gacagattag
    28861 tgattgttga tagaagtacc atcatgatcc ccgcaaaata gacagcactt acgagcacga
    28921 tatgcgcaac cttttggaaa aacagtcgcg gaatgttatc taaaaatcga ttgattttga
    28981 ctcctgtatg tgtgatgaac aatagaagtc ccgtcataaa aggtagaaca taataaaaca
    29041 accgataata taatagccaa actactgcta catcttgccg aacagacaat tgactcattc
    29101 ctaagatcat caaaacgtca aaagtcccca ttccacctgg gaccatcgtc agcattccta
    29161 tcaatgtagc aatcatgaac ataggataga tcgaaagcat cgaaatatct attctcatca
    29221 acttgccgat gatcaaaaat accgcaaggg ctcccgtcca ttgtccaaag gatgccagta
    29281 gcagaccaag caagcctttt ggataaaact cgaagaacaa tgtctttctc tttagataag
    29341 caaaaaagaa aatgactggt gctaaaaaac taccgccgaa aagccagatc caatagcttc
    29401 gaaaaatact ttctgactga ataaaaaaga catcaataaa tgtaagaaac gaccatacag
    29461 acaaaccagt cagcataaat agtgcgactt ttgatacagt ggctaaaacc atttttctat
    29521 ttgtctgctt tccataaaaa ctagcccgca gacttgctcc taccacacca ccaaaaccgg
    29581 cgagattgtt gatcgtgttt gttacccacg cagcaagaag aaactctttt ctttccattt
    29641 ttggttttcc ttgtttttct aagactctga tcgtcaccca atcatacaac aacatcggag
    29701 tgacacccag cagtccagcc agaatcatga gcaggatcgt catgcggctt tgttgtccca
    29761 tcctttgcca aatatcttgc caactcatgc cattagcaat atttgcgact tgatttgcca
    29821 caaagactaa cacggagccg aaaaaaatca gttttaaaat cagactatgt tcctttatcc
    29881 attgcagcat caagtatcgt ttatttttca cattttccca ttcctaactt gtatactttt
    29941 gtgtcatacc ccaatgttat cttatttatt ttaatttttc cactttagac ctgttctttg
    30001 cttgcgatga tagaaaaaga aagatatcct aatgctaaag gagaggataa catgaaagta
    30061 ggatttatcg gcacagggaa tatggcacaa gctattattg atggtttatt aaaaaaagga
    30121 acagtctcga aagaagaggt gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt
    30181 gctgctgaaa ctggcgtcca tgcatgcaaa agtaatcagg aagttgcatc acaatcagat
    30241 atcgttcttt tagccgtgaa accaaaaatc attgaaaaag tattggaaga actaagagaa
    30301 tatagccaga aagtcttatt catttctatt gcttcaggaa aatcattaat agatttagaa
    30361 acagcgcttg aagatcctca ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc
    30421 gtaggagcag gcgtttctgc gatttgcaaa aatgaagctg ccacggagga acagcttcag
    30481 caagctacga tgatttttga agcaatcggc agcgtctatc ctttagcaga atcagatttc
    30541 agtacattca ttggccttgc tggcagctca cccgcattcg tttacatgct gatcgatgcg
    30601 atgggacgga ctggtgtact gaatggtttg tccaaacaga cagccactga gattgctgca
    30661 aaagcaatta tgggttcttg tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt
    30721 gtagaccaag tttcttctcc tggcggtaca actgttgcag gtgtagttgc cttagaagaa
    30781 gcaggattca tcccagcaat catcaaagga atcaatacga cgatcgagaa agatcttgag
    30841 atgttgaata aaaaataatg atagcaatgt tttttcgtgc cttacaaaaa aagaagagct
    30901 gtggcttttg ccgcggctct tcttttgtat tttttattat ttagcataat cagttgcacg
    30961 agtttcccga ataacgatta ctttgatgtg tccaggataa tccagttctt cttcgattcg
    31021 tttacgaata tcacgtacta aacgaacagc gtctaaatcc gagatttctt ctggcttgac
    31081 cattacccga acttcgcgtc cagcttgaac tgcgtaactt gattctacac cggcgaagct
    31141 gttcgaaata ttttccagac ttcttaaacg acggatgtag ttttctaatg attcacttcg
    31201 cgcaccaggt cttgctgctg agagcgcatc tgctgcagct acaaggacag agataacaga
    31261 agtagcttcc acatctccat gatgagaagc gatcgcgttg attactactg ggttttcttt
    31321 gtacttggca gctaattccg caccgatctc aacgtgagat ccttcaatct catggtcaag
    31381 tgctttccca atatcatgga gcaacccggc acgttttgct aattggatat cttcgccaag
    31441 ctctgctgct aagataccag ctaattttgc aacctcgacc gaatgcttca agacgttttg
    31501 tccataacta gttctgaatc gcagacgtcc gatgatcttg attagatctg gatgaagtgt
    31561 atgagcaccg acttcaaacg ctgcctcttc cccgtattcg cgaatacgtt catccatttc
    31621 tttacgagat ttctcgacca tttcttcgat acgcgctgga tggattcgtc catcttgtat
    31681 cagtttttcc aaggtcatcc gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac
    31741 aactgcttcc ggtgtatcat cgatgattaa atcgatacca gtcagcgtct caagcgttcg
    31801 aatattacga ccttctcgtc caatgatacg acctttcatt tcatcattcg gaagggtaac
    31861 gactgaaaca gtcgtttctg aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa
    31921 taagtttttc gctttgcgat ctgcttcttc tttggcacgc tgttcagatt cttttaccat
    31981 tagagttaac tcatgattca gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc
    32041 ctcttttgat aaaccagcaa ttttttctag ttctttttgt tgatcttcaa tcattttttc
    32101 aacgtctttt tctcgctctt caattaattg ctgttttgaa ctaagtgctt cttctttact
    32161 ttcaagtgag ttctcgcgtt tttctaacga gtcatcttta cgattcagcg tttgttcgcg
    32221 ttgaatcaaa cgattctctt gagattttaa ttctaactta ctttctctca gctcactttc
    32281 aacttcagaa cggtatttct gattttcttc cttagcttct aacaatgctt ctttttttag
    32341 agtttctgct tctttttttg cactatcgat gattccagca gctgatgatt gtgcaccagc
    32401 tatttctttc tcgtgacgtg actttgcgac cataaaccct aaaccaagac cgacaattaa
    32461 accgatgata gcgaggagaa tgttgaatac cataaaatcc acctccatac tatctttttc
    32521 atttttatca tctatacagc ttctatgata aattattcta aactcgtcaa tatgcatctt
    32581 cgcatatatg tgtacaaaaa acatgcacaa ctttattcta atgtttgtac cccgtagtgt
    32641 caactttaaa taaacaaaga aacggctttc tacttattca ttaaacgatg gtttttaaaa
    32701 taaatgacac tagtttcata taggtaaacc tttaaattga gaaacttttc tattaaatta
    32761 ttctttgtag atgaacagat atttttattt tatgtaccca ctcgtataat taaataactc
    32821 acgatttctt tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca
    32881 gccgtttttg tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt
    32941 tcatcttctg ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa
    33001 acttctgcca tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga
    33061 ccaatccgat cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct
    33121 actgccatgt ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact
    33181 tcagcgattt tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta
    33241 ccaacaatat ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt
    33301 gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct
    33361 actttttcac ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca
    33421 gagagtttac gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc
    33481 atttcgccgt cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata
    33541 tctacagccc cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca
    33601 ggctgtgaaa gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct
    33661 aacgcatgct cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata
    33721 gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt
    33781 ccacgggggt aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt
    33841 gtagagattt gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc
    33901 ttttcaattt ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata
    33961 atcctcctta tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag
    34021 tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt
    34081 tttctcaaaa gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg
    34141 gcaaagtgat atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt
    34201 cctgcagatt gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa
    34261 tctgttcttg ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact
    34321 gttccatatt ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc
    34381 gagtaggtaa cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg
    34441 gcttggaagg cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc
    34501 agtagttcca ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc
    34561 cctactcgtt cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca
    34621 tcatttgttt tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc
    34681 gttggattga tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca
    34741 atcccataaa agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct
    34801 agtaaaggac gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga
    34861 aggatatacg catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt
    34921 ggtagaggaa ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt
    34981 cgatgggttg tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga
    35041 gacttaccga ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat
    35101 ccgccacata agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa
    35161 cgctgctgat tatctcctac aactgtttga taataaactt cgatacctag atcagctagc
    35221 tgttctgaca agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg
    35281 acagctatta tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt
    35341 tatatttttt tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct
    35401 tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa
    35461 aacatgaaaa gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca
    35521 caacctctcc actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga
    35581 agtaatccac acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta
    35641 ctggaatacc gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca
    35701 tttgtgtagc cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga
    35761 caagtagtaa acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc
    35821 aaccaggggc ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat
    35881 ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt
    35941 ttccatctaa ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg
    36001 tgacagccag tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa
    36061 ccaaaatgaa aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca
    36121 cactatcgac tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt
    36181 atttactttt ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga
    36241 attaacgaag aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag
    36301 taaacagatg cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca
    36361 ttttttggac tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg
    36421 atcgttgcat tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac
    36481 acggtactct gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact
    36541 agcacaccta accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg
    36601 tctgtgatat ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc
    36661 ttttcttcac ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt
    36721 tgctctgagg aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct
    36781 ccgattattg gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc
    36841 gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc
    36901 gattcttcct gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca
    36961 gggaccgatc ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct
    37021 tctcctacag cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat
    37081 tggtcaaaat catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc
    37141 tgtaattcat ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg
    37201 gtttcaaaag ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt
    37261 tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt
    37321 ccgaataaac attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata
    37381 taagaagctg ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac
    37441 ctctttctta aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa
    37501 tataaggaga tatttttccg tatgtttctc taatactcag gtcaaaacgc tgatttcaca
    37561 acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta
    37621 aaaacattaa gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt
    37681 tcacaacctc ttggtacacg gtgttccaga agtagcttct tcggcaataa gccgaaatat
    37741 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    37801 ctgtcacaac ctctttgatt acttcgtttg attttatttt aatataagtt gattaaaaga
    37861 gagcatgatt tgttttattt tctcactaat ttttccaaca caactaagaa taccataaaa
    37921 gctgattttg gaggaggttc ctactacaag tctaaaacga aatttaagac ttttcctcat
    37981 aaaatactgg tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat
    38041 gcttccccag ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca
    38101 aataaagttc gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa
    38161 tttaatgatt ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct
    38221 gacacttccg gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc
    38281 tctggttcgt ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat
    38341 tcaaaaccaa agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa
    38401 gtattgccta aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct
    38461 gttccttctt gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta
    38521 atggctgctt ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt
    38581 acaatttctt gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc
    38641 aatttctcag gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac
    38701 gtccgatagc acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc
    38761 gcaatatcga tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc
    38821 cgccagttag ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg
    38881 tttacagttt cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc
    38941 ttttctacct gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca
    39001 ttggcggaag ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac
    39061 aacttatgtt ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca
    39121 tagggaataa attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg
    39181 tgatagtcat ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa
    39241 agtgtttcat tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa
    39301 gaagattgcc tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct
    39361 ctccatacgt ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa
    39421 aaaagaagct tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag
    39481 cattgctttt gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa
    39541 ttgctcgtga atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac
    39601 actcatgaat cctcggaacg tatccacgct actagaggca taataagcta gactttcttt
    39661 ctctctgaca ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac
    39721 cattagcgca aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag
    39781 ctttgcttga acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg
    39841 ggtataaaac agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc
    39901 ttcagctgct ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc
    39961 catcatttct tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac
    40021 tacgcccaac cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc
    40081 ataggaagca aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt
    40141 cttttccaag tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa
    40201 aacctctttt aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc
    40261 attcacaaaa ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa
    40321 tccaaagctt gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccgaata
    40381 attcaaacta tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg
    40441 ttgcagacgc gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa
    40501 gacatgaaga ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca
    40561 gttgattgcc cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct
    40621 tgaaatttat aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta
    40681 tagcatagat gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat
    40741 tttttatttt tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag
    40801 aattcaaaga gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg
    40861 gtagtgcgtt tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag
    40921 gttggtttgt cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg
    40981 actggtcgcc gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta
    41041 catttctgat cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga
    41101 atttacgctc caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc
    41161 ttagtgacat acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag
    41221 agcaaacaaa tctttccgat acttctaaat aataaagaat ctaaaaaaaa gaaagccgat
    41281 tttcagtgtc ggctttcttt ttttagattc ttaacttgct acttgttcct ttttcatgtc
    41341 gatatgtgga atatcatctt ccaaatacac atctgagatt gccttgaatc caaacgactc
    41401 ataaaaagct tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt
    41461 ctggatcatg ttaatcgttt cttgaacaat ctttcttcct aaaccatctt tacggttgtc
    41521 ctgatgaaca aggacacgtc caaaatggat actctccgct tcttcatata ctcgcgtgta
    41581 ggcaaggatc ttcccatcag tatcttgaaa aaacgtatgc caagcttctc catcaatgtc
    41641 atcaacttct tgataaggac actgttgttc cacaacaaaa actgcgattc gttctttgac
    41701 gatcgcgtaa aactctgctg ttgacaattc ttcaaatttc tttacctgat attccactaa
    41761 gcaaacgccc cctgcttttt tattcttagc ataccgattc gcacacaaaa aagcaaacga
    41821 accttacgaa tctcaggctc gtttgctttt taaactgctt cagttaaaaa ttcgatctca
    41881 actgtccgag taagtacatc tgaatagctg taagatactc gttcaaaaga attttcttca
    41941 ggatccaaat caacaacgaa tactgaagga taggtttctg ttaaaacgcc gcggcgttct
    42001 gtttgacgct ttcttcctgt ctgagcaacc aacatgattg ggctgccgat acgaccttct
    42061 aaatctttct tgattgaagc taaagttgtt ggcatacagt tcacctctac tatcaactat
    42121 accacatatt tttaaaattt tcaagtttac cataaaaaaa aaagaaatgc aataaaaata
    42181 aataagaagc tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt
    42241 tttattttga gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa
    42301 cattcagcca tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct
    42361 atttttgaaa actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt
    42421 gctaaaggtt cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt
    42481 tggtcgattg agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac
    42541 agagaataga tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg
    42601 cttttttcgt aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc
    42661 tcagaaacat aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat
    42721 ttttgataca gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc
    42781 tcagacataa aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt
    42841 catcgaactg attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg
    42901 ggacaatttt tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg
    42961 catatcttta ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc
    43021 ataagcagaa gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct
    43081 tgtcgccact gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata
    43141 actgtcagcc gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt
    43201 ttgttggatg cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata
    43261 tttcgcataa tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg
    43321 ctttttaagc tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa
    43381 cagctgtttt ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa
    43441 accagcagca acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa
    43501 ttcatccatt tcttttttca atcctggact cattcctttg ccttcattac caatgactaa
    43561 agcaattgat cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa
    43621 tacccaaaat cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc
    43681 aacagggaca tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac
    43741 tgctcggtgt tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat
    43801 cgacccaaaa ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt
    43861 tttctctttt gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc
    43921 aagaacgact ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac
    43981 ccatttgact ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc
    44041 tcgtgaatct tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc
    44101 ggcatggtgc ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga
    44161 ctgatttcgt ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc
    44221 acgtttcgat tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa
    44281 tacaccagcg gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca
    44341 tgagcgcttc gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg
    44401 tatggctttt agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat
    44461 gttcttccag catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg
    44521 ccgcacgatg cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat
    44581 agatttcgta aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat
    44641 ctctcatgct tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt
    44701 ttctttcaac aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg
    44761 atttcgttct tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa
    44821 aataccgaag atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc
    44881 agaaactttc ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt
    44941 gatcccattt gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt
    45001 ttctaacaaa gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg
    45061 gaatttcaaa ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat
    45121 cgtcgtttca ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa
    45181 gacttgtgga tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt
    45241 actcattttt tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc
    45301 gaattttttc cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc
    45361 taaatcttgc ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac
    45421 ggaacattca atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc
    45481 gccaggtttt gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc
    45541 ttcccctgta cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt
    45601 cccataattt tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata
    45661 gcctttttca accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct
    45721 tggatgcagt gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat
    45781 gaaacgatca gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt
    45841 atcgtctaca tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata
    45901 gcgtcgaatt gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata
    45961 cacagtcggt ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc
    46021 tttttttctg gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct
    46081 ttgattcgaa cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc
    46141 aacacaacgg cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc
    46201 tgcgcattcg cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct
    46261 tttcctgtgc cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg
    46321 gtttcgccaa tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct
    46381 cctggatgga tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat
    46441 aaaaagagcc ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa
    46501 ccagggtatg tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct
    46561 gcatcaatcg ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg
    46621 taggaaatca tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa
    46681 gacgctttcc gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg
    46741 cctgttatac gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt
    46801 ttcgccaaga ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc
    46861 ggtatctctc ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt
    46921 acttgattta aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat
    46981 tctggaccat gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct
    47041 tttacgccag tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact
    47101 acttccattt gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca
    47161 gcaagtactt cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac
    47221 agatcaacga tttctgcagc atagctcatt ttaggttgat acaggctaac gatcttcttc
    47281 acccattcga ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa
    47341 ggcagacaca tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc
    47401 cattgcagtt ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac
    47461 attttgatca attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat
    47521 aatgcaataa agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata
    47581 aattgcaaga ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc
    47641 aatgtcatat gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt
    47701 tttggtgtat tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga
    47761 tcatcgaccg caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag
    47821 tcgccaccga tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg
    47881 tattctgtat tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt
    47941 tctgcttgtt cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct
    48001 cttgcgcgtt gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat
    48061 gctcgattgc tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat
    48121 tgacggtaag ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac
    48181 caagcaaggt tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta
    48241 tcctcaatac gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat
    48301 gcagttcttg catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt
    48361 actttcgtca ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg
    48421 tcagttacgg cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt
    48481 ctaaggtaaa tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt
    48541 ttattttgat tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc
    48601 atgtgcaggt ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac
    48661 gacttcgata cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc
    48721 atcaaggtag gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat
    48781 cgtttcacca gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac
    48841 aggtacattt tggaattcag agactttatt caagttatag tcattggtta caatgacacc
    48901 atctaaaagc ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc
    48961 tccatcgtac atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa
    49021 tccgcgacga ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc
    49081 gtaaagaaca aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc
    49141 ataaatccga ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc
    49201 tcctgatttt cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt
    49261 tttgcttcgt gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa
    49321 tcccagatag cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat
    49381 ttccatattg taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc
    49441 gatcgcaccg aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt
    49501 gataatagct gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc
    49561 taacaggctg ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg
    49621 ccaagctgag ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt
    49681 aatgacacgt ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa
    49741 cacacgcttc aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc
    49801 ttcccagtta cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac
    49861 ttctttgact cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc
    49921 accgatgaaa cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac
    49981 agcgatagct aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta
    50041 agcatcttga ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga
    50101 tacccgattg aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt
    50161 gactaatgct tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc
    50221 agagccagtt gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc
    50281 ttccaatcca tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt
    50341 ttttactgct cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt
    50401 atccaccata tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc
    50461 gacgataaaa atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc
    50521 tcttacttgg ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat
    50581 ggaatcaatg ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat
    50641 atctgtttca gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa
    50701 ttttatctgc tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc
    50761 taattgctga gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat
    50821 caagaccata gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt
    50881 tgttttgatc cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt
    50941 ttctcctgtc aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac
    51001 catcgtattc cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc
    51061 gcaattttgg caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat
    51121 cctaatcatg agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa
    51181 ccaaatccgc cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa
    51241 aagatccctt ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg
    51301 gtgaattgga acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc
    51361 acacctactc cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca
    51421 agctgcaaaa attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt
    51481 ttcaattgtt tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac
    51541 ccctttttcc agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa
    51601 tcatagccag cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat
    51661 ttagaaataa cttcaaatcc tctttttctc accggttttc ctccctaaat aaactactcc
    51721 tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa gaaattctgc
    51781 tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt gtacaaaaaa
    51841 actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg
    51901 gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc
    51961 aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc ggcaatcgat
    52021 cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat cttagtctca
    52081 tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc gactttgatt
    52141 cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat ctataatcaa
    52201 agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga cgtggtagat
    52261 tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat tatggacacg
    52321 atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca tctgacaaaa
    52381 tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc agaccttttg
    52441 atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa tcccgaggct
    52501 cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc aggtggtgct
    52561 ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc taggatattt
    52621 gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat ttttgatatt
    52681 cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact gacttgatgc
    52741 aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct acaaaccgca
    52801 aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg
    52861 aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag
    52921 accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc gaaaaaattg
    52981 cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc gacggattga
    53041 gaatcggcaa aaagcgcgtg ccactaggcg tgatcggcat catttacgaa tctcgtccaa
    53101 atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt atccttcgcg
    53161 gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag caagtactgg
    53221 tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct catgaaactg
    53281 caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga ggcggagcaa
    53341 aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca ggtacaggaa
    53401 ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac atcatcgtca
    53461 atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg atccattctg
    53521 agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac ggggtctctt
    53581 tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca gaagacacag
    53641 attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat tctttggatg
    53701 aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc gtgacagata
    53761 gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg
    53821 cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt ggtatctcta
    53881 ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc acaaaatata
    53941 tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt attcccgcct
    54001 tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg tttgaaattt
    54061 taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg ggagtcggta
    54121 ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt
    54181 cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac attagttaca
    54241 tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat cattcaatcg
    54301 cttatacgat tacgctactg attgctggga tttttcactt atcttataaa tatcagctga
    54361 ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa gtagagtgcc
    54421 ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat tttgtcacag
    54481 cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct gtttcctctt
    54541 cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc agctcatcca
    54601 tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt agctgttccc
    54661 gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt tcaccttccg
    54721 cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca tctgaaaatt
    54781 caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc agcatctatt
    54841 atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc
    54901 attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat
    54961 aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa
    55021 actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg
    55081 gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta aaatatgaga
    55141 agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt tacaacctct
    55201 g
//
LOCUS       DACEDO010000010        54449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEDO010000010 DACEDO010000000
VERSION     DACEDO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.010"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..590
                     /locus_tag="IWO52_000658"
     CDS             <1..590
                     /locus_tag="IWO52_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354948.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(830..913)
                     /locus_tag="IWO52_000659"
     tRNA            complement(830..913)
                     /locus_tag="IWO52_000659"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Leu,seq:gag)
     gene            1296..2513
                     /locus_tag="IWO52_000660"
     CDS             1296..2513
                     /locus_tag="IWO52_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0354949.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2697..3533
                     /locus_tag="IWO52_000661"
     CDS             2697..3533
                     /locus_tag="IWO52_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0354950.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3609..4757
                     /gene="nagA"
                     /locus_tag="IWO52_000662"
     CDS             3609..4757
                     /gene="nagA"
                     /locus_tag="IWO52_000662"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0354951.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4978..6036
                     /locus_tag="IWO52_000663"
     CDS             4978..6036
                     /locus_tag="IWO52_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0354952.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6055..7074
                     /locus_tag="IWO52_000664"
     CDS             6055..7074
                     /locus_tag="IWO52_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0354953.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7176..7892)
                     /locus_tag="IWO52_000665"
     CDS             complement(7176..7892)
                     /locus_tag="IWO52_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0354954.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7861..8646)
                     /locus_tag="IWO52_000666"
     CDS             complement(7861..8646)
                     /locus_tag="IWO52_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0354955.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8637..9386)
                     /locus_tag="IWO52_000667"
     CDS             complement(8637..9386)
                     /locus_tag="IWO52_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0354956.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9684..10208
                     /locus_tag="IWO52_000668"
     CDS             9684..10208
                     /locus_tag="IWO52_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354957.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10224..11723
                     /locus_tag="IWO52_000669"
     CDS             10224..11723
                     /locus_tag="IWO52_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0354958.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11811..12413)
                     /locus_tag="IWO52_000670"
     CDS             complement(11811..12413)
                     /locus_tag="IWO52_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0354959.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12517..13473
                     /locus_tag="IWO52_000671"
     CDS             12517..13473
                     /locus_tag="IWO52_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0354960.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13778..14734)
                     /locus_tag="IWO52_000672"
     CDS             complement(13778..14734)
                     /locus_tag="IWO52_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0354961.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14908..15468
                     /locus_tag="IWO52_000673"
     CDS             14908..15468
                     /locus_tag="IWO52_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354962.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15461..16286
                     /locus_tag="IWO52_000674"
                     /pseudo
     CDS             15461..16286
                     /locus_tag="IWO52_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            16296..16913
                     /locus_tag="IWO52_000675"
     CDS             16296..16913
                     /locus_tag="IWO52_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0354963.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16931..17614
                     /locus_tag="IWO52_000676"
     CDS             16931..17614
                     /locus_tag="IWO52_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0354964.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18297..19580
                     /locus_tag="IWO52_000677"
     CDS             18297..19580
                     /locus_tag="IWO52_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0354965.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19596..20483
                     /locus_tag="IWO52_000678"
     CDS             19596..20483
                     /locus_tag="IWO52_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0354966.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20500..21336
                     /locus_tag="IWO52_000679"
     CDS             20500..21336
                     /locus_tag="IWO52_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0354967.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21339..21962
                     /locus_tag="IWO52_000680"
     CDS             21339..21962
                     /locus_tag="IWO52_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354968.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21959..23251
                     /locus_tag="IWO52_000681"
     CDS             21959..23251
                     /locus_tag="IWO52_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0354969.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23610..23707
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23823..24404
                     /locus_tag="IWO52_000682"
     CDS             23823..24404
                     /locus_tag="IWO52_000682"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0354970.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24407..25714
                     /locus_tag="IWO52_000683"
     CDS             24407..25714
                     /locus_tag="IWO52_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0354971.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26130..26618
                     /gene="purE"
                     /locus_tag="IWO52_000684"
     CDS             26130..26618
                     /gene="purE"
                     /locus_tag="IWO52_000684"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0354972.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26602..27735
                     /gene="purK"
                     /locus_tag="IWO52_000685"
     CDS             26602..27735
                     /gene="purK"
                     /locus_tag="IWO52_000685"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0354973.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27748..29043
                     /gene="purB"
                     /locus_tag="IWO52_000686"
     CDS             27748..29043
                     /gene="purB"
                     /locus_tag="IWO52_000686"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0354974.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29223..29600)
                     /locus_tag="IWO52_000687"
     CDS             complement(29223..29600)
                     /locus_tag="IWO52_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354975.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29856..30584
                     /locus_tag="IWO52_000688"
     CDS             29856..30584
                     /locus_tag="IWO52_000688"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0354976.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30584..30838
                     /gene="purS"
                     /locus_tag="IWO52_000689"
     CDS             30584..30838
                     /gene="purS"
                     /locus_tag="IWO52_000689"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0354977.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30840..31511
                     /gene="purQ"
                     /locus_tag="IWO52_000690"
     CDS             30840..31511
                     /gene="purQ"
                     /locus_tag="IWO52_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0354978.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31512..33734
                     /gene="purL"
                     /locus_tag="IWO52_000691"
     CDS             31512..33734
                     /gene="purL"
                     /locus_tag="IWO52_000691"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0354979.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33719..35158
                     /locus_tag="IWO52_000692"
     CDS             33719..35158
                     /locus_tag="IWO52_000692"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0354980.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35190..36233
                     /locus_tag="IWO52_000693"
     CDS             35190..36233
                     /locus_tag="IWO52_000693"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0354981.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36230..36808
                     /gene="purN"
                     /locus_tag="IWO52_000694"
     CDS             36230..36808
                     /gene="purN"
                     /locus_tag="IWO52_000694"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0354982.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36805..38340
                     /gene="purH"
                     /locus_tag="IWO52_000695"
     CDS             36805..38340
                     /gene="purH"
                     /locus_tag="IWO52_000695"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0354983.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38356..39630
                     /gene="purD"
                     /locus_tag="IWO52_000696"
     CDS             38356..39630
                     /gene="purD"
                     /locus_tag="IWO52_000696"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0354984.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39752..40666
                     /locus_tag="IWO52_000697"
     CDS             39752..40666
                     /locus_tag="IWO52_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0354985.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40799..42364)
                     /locus_tag="IWO52_000698"
     CDS             complement(40799..42364)
                     /locus_tag="IWO52_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0354986.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42357..43808)
                     /locus_tag="IWO52_000699"
     CDS             complement(42357..43808)
                     /locus_tag="IWO52_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0354987.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43822..44838)
                     /locus_tag="IWO52_000700"
     CDS             complement(43822..44838)
                     /locus_tag="IWO52_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0354988.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45058..46146
                     /locus_tag="IWO52_000701"
     CDS             45058..46146
                     /locus_tag="IWO52_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0354989.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46200..47726
                     /locus_tag="IWO52_000702"
     CDS             46200..47726
                     /locus_tag="IWO52_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0354990.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47728..48933
                     /locus_tag="IWO52_000703"
     CDS             47728..48933
                     /locus_tag="IWO52_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0354991.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49190..50167
                     /gene="guaC"
                     /locus_tag="IWO52_000704"
     CDS             49190..50167
                     /gene="guaC"
                     /locus_tag="IWO52_000704"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0354992.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50431..51855
                     /locus_tag="IWO52_000705"
     CDS             50431..51855
                     /locus_tag="IWO52_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0354993.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52316..53425
                     /gene="pdhA"
                     /locus_tag="IWO52_000706"
     CDS             52316..53425
                     /gene="pdhA"
                     /locus_tag="IWO52_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0354994.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53428..54405
                     /locus_tag="IWO52_000707"
     CDS             53428..54405
                     /locus_tag="IWO52_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0354995.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17400 a         9316 c        11922 g        15811 t
ORIGIN      
        1 tttttttaga catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat
       61 ataggaaatg tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc
      121 tgattattta ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa
      181 gggcaatgat ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac
      241 tgtcactttt ttatccaggt acttggatag ctactttagt agtcttggca ttattactat
      301 gtttaattgt tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta
      361 ttttgatgat aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg
      421 cttccggaat gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg
      481 cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat
      541 taaccgtgct tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa
      601 atccctcgct ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca
      661 cttgcaagtt tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta
      721 catgattgta cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa
      781 cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg
      841 ggactcgaac ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt
      901 ccaacacatc tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag
      961 ctcaaaaatg ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg
     1021 aatgatctta ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct
     1081 aggaatcatt tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga
     1141 caattaagtt ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt
     1201 ggcaaaattt tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa
     1261 gttctaactt tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca
     1321 aattagaagg tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc
     1381 aagaagaggg cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg
     1441 aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact
     1501 cagattccaa ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac
     1561 attttccgaa tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca
     1621 cgatgtgtat gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact
     1681 atccgttgtg gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg
     1741 atgaagaggc agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga
     1801 caaaagctat tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat
     1861 tattagaaga aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa
     1921 tctatgatcg cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg
     1981 atttgttcgt tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag
     2041 taggctggat ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga
     2101 atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg
     2161 cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac
     2221 aaagagaata tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa
     2281 aagctgcttt ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg
     2341 aaaaattcgt tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat
     2401 ttaattggaa ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga
     2461 aacaaacagc taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa
     2521 aaccaaattt ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga
     2581 gttttcttaa ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac
     2641 ttcctaaagt aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg
     2701 aaacgttaat cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga
     2761 cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata
     2821 atgaggaact gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga
     2881 ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac
     2941 tgttggatag ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt
     3001 ctgatatttc tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg
     3061 gtgtttgcga attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag
     3121 cattaagcta ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga
     3181 ttagttgtat cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt
     3241 tgagtcgaag catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt
     3301 cgcaaatttt ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt
     3361 tagaacaggt gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca
     3421 atgatttatt tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag
     3481 cattctatga aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt
     3541 agaccattct tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag
     3601 agaatagaat gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac
     3661 atgcaggcta tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg
     3721 agggagaagt caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata
     3781 cacatattca cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag
     3841 tgatgtctga aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt
     3901 caagcaaaga acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac
     3961 caggagctaa aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag
     4021 gggcacaaaa cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag
     4081 aagcttcagg tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag
     4141 agtttgtcaa acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta
     4201 cactagaaga agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca
     4261 atggtatgcg tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag
     4321 aacatgtctt ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa
     4381 tcctaatgga aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg
     4441 gtgggatgcc tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa
     4501 cagcacgtct agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta
     4561 aaaatgttgt agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga
     4621 ttccagcaat cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg
     4681 cagactttat cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg
     4741 aacgtttcca cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt
     4801 tttaaggttt tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt
     4861 aatttcctat tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat
     4921 tagcaaaata aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg
     4981 aacttatttg acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact
     5041 tcaacagttt ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa
     5101 tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt
     5161 cgagctttca tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt
     5221 tggggattag ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat
     5281 aaaggggata agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc
     5341 ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc
     5401 aatattggct accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag
     5461 atggaagcta aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc
     5521 ggccttttca tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa
     5581 ggagagataa gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag
     5641 ccgatggatt atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca
     5701 ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta
     5761 gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg
     5821 aatattctac cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca
     5881 gctgcaacag tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc
     5941 aatgcatcct tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta
     6001 ttacaaatga ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga
     6061 atcgcatgtt acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac
     6121 cattatgatc tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag
     6181 gaccatgatg ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa
     6241 atgcaggaat atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat
     6301 ctacaagcgg cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat
     6361 gcgatagcag agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg
     6421 acgatccgaa catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc
     6481 agaaattgca cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg
     6541 tttaagggac taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa
     6601 aaagtattga cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt
     6661 catgtacctc atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa
     6721 atgaaaaaag gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct
     6781 attttaagag ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat
     6841 gaaacagagg tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc
     6901 cagcaattag tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca
     6961 gatgaggcat tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa
     7021 actggaaaaa cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac
     7081 atggatattc gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta
     7141 tacttggact attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc
     7201 cgcccattct ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt
     7261 ctttcgttca atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt
     7321 gacagtgagc ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata
     7381 aatgcactta ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat
     7441 ggtatttcgt gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct
     7501 ttttcagtcg tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa
     7561 cgttgcaaag tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt
     7621 atggtttcga tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt
     7681 ctaagccaag ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc
     7741 agatctgcgt ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg
     7801 attacgttca tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga
     7861 tcatcttcga ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag
     7921 atagacgatt gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt
     7981 gacgattgaa tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc
     8041 tagcgtaaat attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt
     8101 cacgatgaat acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct
     8161 ttcatgtttt tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa
     8221 aagtgaatgg atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag
     8281 catttcaaca gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc
     8341 aggtactttg atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg
     8401 ctggataaaa tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg
     8461 aagtttattc atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc
     8521 cccaagctcc tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc
     8581 taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa
     8641 atacatggat tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc
     8701 atatagatta tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag
     8761 aaaatgtttt ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag
     8821 attccttcat ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct
     8881 tgtggttcaa gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg
     8941 atgcctacgc taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact
     9001 tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca
     9061 taataaatag ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa
     9121 aattcttgtc ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg
     9181 taacttgggt ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct
     9241 aaattcgatg caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca
     9301 gtctgcagct ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt
     9361 ttatctgaca cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa
     9421 acaatcaaag aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt
     9481 aatggttttt tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat
     9541 tttaacatga aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa
     9601 aacaccactg agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg
     9661 tagaaatgga aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt
     9721 atacattgct gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg
     9781 attttgatgg cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat
     9841 ttggttttct aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg
     9901 gaggcatatc gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac
     9961 atgccgaatc aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga
    10021 tcacaccgat ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca
    10081 ttagtcgtcc agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag
    10141 tattgatcat tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa
    10201 aagcttaaag gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg
    10261 cagctttgat tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac
    10321 cggatgaagc gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg
    10381 aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc
    10441 aacgttcgaa tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt
    10501 atacggaaca aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat
    10561 ctgtagaaga gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg
    10621 aaaatgaagc aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg
    10681 tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg
    10741 tagaccttgc aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca
    10801 aaaacggtta tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag
    10861 atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag
    10921 aatcacaagc agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag
    10981 aactgaaatt agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag
    11041 cctacaattt agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta
    11101 aaatcgtaga acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa
    11161 agcaatatga ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag
    11221 aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg
    11281 aagcactggc agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag
    11341 cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca
    11401 aggaatatgg cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc
    11461 gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca
    11521 gttcaggaga aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt
    11581 ctactcaaga aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt
    11641 tgtcgacacg tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac
    11701 aaaactcgat gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa
    11761 gactttttaa agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg
    11821 ctatttttct tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat
    11881 cgttctttct tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca
    11941 ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt
    12001 acacgttgga tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac
    12061 aacgtagcta ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc
    12121 acaataataa ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag
    12181 cttttgacaa aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta
    12241 gactttccaa aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat
    12301 gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca
    12361 gctaaggcac ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt
    12421 ctcctttttg gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt
    12481 attcaagtat aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc
    12541 aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat
    12601 tggccagctg cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg
    12661 cgtacgggca tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt
    12721 ttttttgctg ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg
    12781 cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat
    12841 tatttagtgg aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg
    12901 attggattag tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg
    12961 aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa
    13021 tttcttttat cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc
    13081 attcgcgaaa gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg
    13141 ctatactacg aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg
    13201 ctggaaaatc tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta
    13261 ttgatttata ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt
    13321 attgaagaag tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc
    13381 gaggatcgag cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag
    13441 gcagaattct cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg
    13501 tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc
    13561 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
    13621 ggagctgaac ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc
    13681 ggtaataagt ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
    13741 acttgatgca gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt
    13801 gcatttgatc caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt
    13861 caccttcaat catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc
    13921 cgctttcgat gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg
    13981 ggtaatcagc aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt
    14041 ttgcctgaaa agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga
    14101 cggattgttc gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt
    14161 gagagctcaa aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc
    14221 ctccagctaa ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga
    14281 aaggtgcttg gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact
    14341 gctgataaag tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt
    14401 ctgctaagcg catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata
    14461 gaacgtgttt gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta
    14521 cagcaatgta cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag
    14581 cttttggaat attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa
    14641 ctgctgttag ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac
    14701 caagaccaac gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat
    14761 tgagtttctt gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact
    14821 tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag
    14881 tagaatagtt aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt
    14941 gtattattag aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt
    15001 ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag
    15061 aaagtaaaaa atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa
    15121 acgattattt ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca
    15181 cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa
    15241 aataatcagc aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg
    15301 ggctatgatg atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga
    15361 gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag
    15421 gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt
    15481 tggagcagtt gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc
    15541 gcatgttcaa gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa
    15601 agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac
    15661 gtccaaacaa ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga
    15721 tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata
    15781 cttgatcgac aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc
    15841 tggatttcca ttagtgatgg tctcagctta ccggtctgtt atcatcccaa caacaagttt
    15901 tcgaacaaaa tgtccaagat gtgatgagtc gtcaaggtgt atcagaagag gaggcgacaa
    15961 agattactaa agaaacgatt acggagccag gatacagcga acaccacaca ggtctggcag
    16021 tcgatgtcgt cgatcaaaac tggtacaaca gttatccttc tactgtattg gatgcaagtt
    16081 acggagatca gcctggagct aaatggatcg cagataatgc tgcgcagtat ggatttatcg
    16141 ttcgttatcc aaaaggacac gaagatataa ctaagatcac atatgaacct tggcacttac
    16201 gttatgtcgg taaagaaaac gctgagtata tcaccaaaca ccaattgacg ctagaagaat
    16261 atctgaatga attaaatgaa aagtaggaat tgatcatggc gaaaaaaaag tggaaaaagc
    16321 aaaagcgcaa gaatatccgt tggccagcag tggttgccgg tgtagcagtg atactcttag
    16381 cctttgtttt ttccttgaag aatctgtctt ctccttttac tgataatcag caagaaaatg
    16441 aagcgcaatc ccatcagcaa tttattgaac ggcttgttcc gcatgcccaa gaactacaag
    16501 atggttatgg agtgcttcct agtatcattt taggacaagc tatattagaa tcaaattggg
    16561 gaaaaagtac cttggctagc aaatacaata atcttttcgg gatcaaggct tatggggatc
    16621 agaagaaagt aagcctggaa acaaaggaat ttgttaacga agaatggata actatccaag
    16681 gagacttcaa agtgtatgat tcatgggaac agtccatgga tgaccataca caactgttcg
    16741 tccaaggagt agattggaat cctgcgctgt atgaaaaagt gattacggcc accaactatc
    16801 aggaagccgc acaagcattg caagatgcag gttatgcgac agaccccggt tatgcgcaaa
    16861 aaatcatcca ggtcatcgag acgtatcaat tgaatcagta tgaccggaga tagcaaagga
    16921 gaagtggctg atggcagaac aatttacgcc agaaatcatg acggaattga gaaaagatat
    16981 cgtaagagta ccggaaatca ttttagaagc aagcgggatc aagatatttg gaaaattgat
    17041 ccgttcgatc atctttacta cggatattgc gatcatacgt aatacaaatg cgaatgcagt
    17101 aattgctgtg tatcctttta cacctcatcc agcgattact aaaagcatta ttgaggcagc
    17161 tgacattcca gttttctccg gtgtaggtgg tggtttgaca caaggtgctc gttcttccta
    17221 catgagcatg ttcgcagaag cgcaaggttc gttaggggtc gttttgaacg gtcctacgcc
    17281 tttagagaca gtaaaagaag tttgtcgagt agttgatatt ccagttatca gtacggtaac
    17341 gtcgaaatat acagaaatag atgaaaagtt agcagctggt atttcgatta tcaatatcag
    17401 tgcaggtaaa gaaactgcag aagcagtacg ccattttcgt aaaaaatatc ctgaacttcc
    17461 tatcattgcg acaggtggtc caacggaaga aagtattgct gagaccattc aagcaggagc
    17521 aaatgccatc acatatacgc caccaagtag cggagagctt tttagtcgta aaatggaaaa
    17581 atatcgggaa actgagaaaa agaatcatga ataacaggtg agaatcaaaa gacgaggaag
    17641 agcagatagt ggctcttcct cgtcttttgt actccagtac tatttgttca gaattcaaaa
    17701 aaaagagaaa tttttttgtt tttttacctg taatgtacat aagaagagtt aaaacctttt
    17761 gttattatta gcattctaac aaaaaaatgt cttgatttat tgaagcgctt tctatagaat
    17821 gaaaaacgaa taaaaactaa aaaaggcacc cgacaattta gtagcagcta aattgtcggg
    17881 gcttgaaaag tcagcgggaa ctgaccaatc aagttgccat agtcaatgct tgtgcatcga
    17941 cgtgtatatt gtacctaatt tcatacagaa ttactagtta tttttctgtt ttttagggaa
    18001 gagcgcacac gaagaagcat gcttatttag ctatgtcaag gatattgggc agttgtctga
    18061 tatccttttt tctttgcgta attatcctcc catcattgaa gcaagaagtg tttttagcga
    18121 tgaaagaaga atcagcacta tctaattttt cttgaaagta ttgagttgtt catcctaggc
    18181 agaacgataa ggcaagtctt ggtttttatt ccccttgaaa aagatcgttt aataaatatg
    18241 ttctgtataa aaatacagta tattgaaaaa taacaatgga agtagaggtg cagagaatga
    18301 agaaaagaat gagaatcatc ttagctttaa gtgttatgtt attcttcttg acggcttgtg
    18361 ggcaagaaga tgacaaaaaa atcacgctga acatggcttg gtggggttcg caagtccgac
    18421 acgatgcaac agtaaaagta atcgagcttt atgaagaaaa aaatccccat gtgacgatcg
    18481 attatgaatt ttatgattat gaaggatatt acacaaaatt aggtacacta gcagcatcag
    18541 atgatatctg ggacattttc caactgaaca atcaatttcc tcaatacatc aatcagattg
    18601 aaccattaga tgaatatgtg gagcaaggga taattgatat ttctaatgca gatgaatcct
    18661 atttagcaac gacgacttac gacgggcaat tagttggact gtctaacggg gtgaattcgt
    18721 ttgcgattgc ttacaataaa aatatatttg aacaattaaa tatccctgaa ccagcttcaa
    18781 attggacatg ggatgaattt gaacaaatca gctatcaaat cactgaagaa ttaggaatct
    18841 atgctatttc aagattcgaa gattttatag ctggctgtat tattcaaata ccacaagttg
    18901 agaaaggatt gaatttttat aatcgggaag atctatctaa cagcttggga tttgaaaatc
    18961 cagactatct aacagatttt ttccagctgc gtaaaaatct ggtagacaaa ggggcccatc
    19021 cagaaccagg tggtggagct gcaagtacga cggatctgtc ttttcaggcg gttagggata
    19081 gagaagcggc aatggtgttt ctatcaagta accagttgac agaaatattg tcaggggctc
    19141 ctgaagatat ggagattcag ttgattaatc cacctcgcag aaaagcagat ggagaatctg
    19201 gtatcccttt acgttcttcg caaatgctga gtatggcaaa aagctcagcc cataaagaag
    19261 aagctgctaa gtttattgat ttctttcaaa acagtgaaga agccaatgaa attttaaaag
    19321 gagaacgtgg tgtaccaatc atgtcgaata ttcgagaaca gctcgctcgt gcagatgatg
    19381 caactttgca agcgacattt gattatttag aaatgatttc cgatatggac aacggcgaag
    19441 tgaatgtctt tgatagtgct ttgaatccgg aaattcaaga ccaatacaat cttttagttg
    19501 aaaaagtcat ttatgaagaa gtaacagcag aagaagcagc agagagtctt tatgagtttg
    19561 cgtcaaacat tctaggatag cagcaggagg aaggcatgaa aaaagaaaaa ctaaaacggt
    19621 tcttggatca agacaatgtg gtcggttacg tatttgcttc tccattcatc ctaggatttc
    19681 tattgataac agtgataccg atgatcttgt cactttatta ttcgttcacc gattatcgtt
    19741 tgggaaatcc agtctcatgg atcggcttgg ataattatat aaagatgctg agtgatgccc
    19801 gttttttgaa ctccatccga gtcacttttc tctatgtatt tgcatctgta cctgtgaaac
    19861 tgatctttgg tctattgatt gcttatcttt taacgaggaa tatcaaagga acgactttct
    19921 ttcgttcttt gtattatgtg cctagtttga tcggtggcag tatttctatc gctctagttt
    19981 ggcgggagtt gttttctaaa aacgggttag tgaatatttt cctggaacga ttaggcgcac
    20041 ctgcgctttc ctggtttagc gatcaaaaac tggtcatgat ccctcttgtt ttgatgtctg
    20101 cttggcaatt cggttcggca atgatcatct ttgctgcagg tatcaaagag atcccaggct
    20161 cgtattatga agcggcagag atcgatggag cgaacaaatg gcagacgttt tacaagatca
    20221 cgttacctct tctttcgccg atcattttgt ataacttggt tatgcagaca atcgcggcgt
    20281 ttatgacttt tacgcaggca tttatcatta cccaaggtgg tccgaatgat gccacgaact
    20341 tttatgctct ttatgtgtat aaccaagctt tttcttttaa ttctatgggg tatgctagtg
    20401 ctatttcgtg gatcatgctg ttgattatgg gagctgtcac ctttctgatt ttcaaatcat
    20461 caaaaaaatg ggtcgttgtg tgaatgagag gaggaagaaa tgaataatac agtaacgaaa
    20521 tggtttcaat acggttttat tttgctatta ggtctagtca tgatttatcc ggtcttatgg
    20581 atgatcgcag gttcttttaa aacggatcaa gagattatga gtggttcgct gaatttgatt
    20641 ccagagactt ttcaatggga aaattacgca aaaggatggg cggggtttgc tggaatttca
    20701 ttttttactt tctttaggaa ttcgtttgtc atctctgcag tttcgacgat agggacagtt
    20761 ctgagctcga cttgtattgc ttacgcatta tcgagaatcg atttcaaagg aaaaagattc
    20821 tggttcgtcg tcatgttagt aacgatgatg attcctgcac aagtgatttt gattccgcag
    20881 tttattattt acaatcggct gaatttggtt gggacgtatg tgccgctgat tttaccacat
    20941 ttttttggac aagcattttt catttaccag atcatgcaat tcatggtcag catccccaaa
    21001 gagttagatg aatcagcgat tattgacgga tgcagcaagt atagtgtctt taccaaaatt
    21061 atttttcctt tgctgaaacc ttcgatcatc acgacgatca tcatccagtt ttattggaaa
    21121 tgggacgact tcatgggacc attgatttat ttaaacaagc cgagatctta cacggtatcg
    21181 attgccatca aattatttgc agacgctgga tctagtacga cttacgcgag tatgtttgcg
    21241 atgtctacgt tgtcgctgct accggttttt ctgattttct tattcttcaa taaatatctt
    21301 gtccagggga tcagtacaag cggtctgaag ggctgaatat gaaaaagcca atagaagaga
    21361 tgtttcttta caagctcaac tactatgtct gtcggatttt tttaaccagt cttatcagta
    21421 atgctctttt cgctttagtt ctaggatgtt tgttcacttt aggattagtg ctgtctgctc
    21481 gtgttgtcta tctgatttat tttttgatga tgtatccttc ttattatcta tctgtccatt
    21541 acttggcaga aaaaaaggta tgttggagga aaggaaaaat caaacggatg tgtttcatca
    21601 ttacgttgtt tcagctgttt gtcattattt ttgcagttga ttggtattta ttagtgggaa
    21661 agattggagc ttggtatctg attccttttc tattgtgtct gttgcttttt gtatcccata
    21721 gtttttttag tacgctaatt gatatctgca aagaagataa agcagatagt cttgtttatt
    21781 tgatcaaaag gaatatctgg ctgttgtcaa aaaacgtcaa ggggtttctg tggtatttat
    21841 gccttttgta tggactgttc tggtgtatac agcatgattt tttactcact gcattactgt
    21901 atccagggct gccatttgtc acagtatttt gcttaaaaga aaaggagaat gactttaaat
    21961 gaagaaaaaa tatgtacaag tcggacttgg tgggagagcg cgtttttttt atgaggcaat
    22021 tgcctctacg tatcatcaaa cttgtgaact agtcggtttt tgtgatgtat ctcaaacaag
    22081 gatggattat gcaaatcgga tacttgtgaa caagtatgga cagaaaaaag taaagacatg
    22141 tatgccatca caatttgagc aactactgga tgacgtacgg ccggctgaag tcattattac
    22201 gtcagtagat cgaacgcatc atgattatat cattcgtgca atgaaaaaag gatatgatgt
    22261 tatttgcgaa aaacctctga ctatcgatga ggaaaaagct caagaaataa taacggtcca
    22321 aaaacagacg ggaagaagcc tgcgagtcac ttttaattat cgttatgcac cgtatcacac
    22381 gaagataaag gaactgatag aaagtgatgt gattggtgat gtgttttcta tccacttcga
    22441 atggctgtta aatacgcaac acggggcaga ttatttcaga agatggcatc gaaataagca
    22501 caatagcggc gggcttcttg ttcataaatc tacccatcat tttgatctag tgaatttttg
    22561 gttgaacagc cgtccccaaa aggtatttgc attcggtgga ttaaactttt atgggatgca
    22621 taatgcggag aaacgcggag tagaaatatt ttacagtcga tgctatggaa gcgaactagc
    22681 taaagctgat ccatttgcta tagacctgtc agccaatgag gaattgcgag aaatgtattt
    22741 agaagcagaa aaagacagtg gatatattcg tgaccgcagt gtattttccg atgatatctc
    22801 tattgaagat acactaggcg taacggtcaa atatgaaact ggtgcgattt tgacgtattc
    22861 gctaaacgcg tatatgccgt gggaaggatt caatgtttct ttcaatggca gcaaaggacg
    22921 gatcgaaatg aaagtgatcg aaaagtctta catcaatgca ggtggagata aaaaggatga
    22981 aggagcagcc gttcttcagt ccatccatgt ctatccgatg ttcggtgagc catatgaggt
    23041 aatcgtcgaa caagaagcgg gcggacatgg cggaggagat ccgcgtttat tagatgatct
    23101 atttggtgaa gaaaaagaag aagatccgtg gaatcgggca gcaactcacg tagatggtat
    23161 tttgtcgatt ttgacaggaa tcgcagcaaa tcattctatt gcttcaggaa aagcagtagt
    23221 gattgacgag cttgtcagct taaaagaata agaatagatt tacggaaatt acttcccgta
    23281 gtttataaaa tcttgcctta ttgccgatag gctgtctttt gaacactatt tattcggaaa
    23341 aaggggctat gacatgcttt ttgtcatgcc ttctttttat gtgggctgct tttttagata
    23401 ggagaaaata aaaataacta tttaaacccg aacaaaaagg aataatgcat taaaaatcat
    23461 tagattttac aaaaaaataa gtaaaccacg ttgtcagttt cagaaagtag tgttagactg
    23521 ttttcaatat ttgaaacaac attttttatt caaatatggc aaactgcctt cacgtgcaaa
    23581 acagttgcaa gaaaatgaat ttacactatt attagtttta catacctact tatatatcgt
    23641 cataatatgg atgacagttt ctagccagta ccgtaaatgc tggactataa gtaaaagatt
    23701 ggctatttca cccttctttt cttatgataa agtggagggt gtttttcttt aacagaaaca
    23761 tagtaggttt ttgcacaaaa gcggttattt cggtattaat aaatgataaa ggagcaaata
    23821 aagtgaaaga attagttgaa cgtattcaaa aagacggacg tatattaggc gagggcgtat
    23881 taaaagtcga cagcttcgtc actcaccaag tagaccctgt attgatggaa caaatgggaa
    23941 aacggtttgc agaagtattt aaagaacaag gaatcacgaa agttgtgacc attgaagctt
    24001 ctgggatcgc accagcttta tttgctgcaa gagaattaga tgttccaatg atcttcgcta
    24061 gaaaagcaaa aagtttgacg atggatgaag aattattgac agcttctgtt tattctttta
    24121 cgaaacaagt gacaagtacg atttctattt ctcgtaaatt tttatcttca gaagacaaag
    24181 tattgattat cgacgatttc ttggcaaacg gacaagcggc aaaaggattg atcgaattat
    24241 gccagcaagc tggagcgcaa gtagaaggaa tcgggatcgt catcgaaaag tctttccaag
    24301 atggacgtca gttacttgaa gaaatgggat tgagagtcgt ttctcttgcg cgtattgctt
    24361 ccttgacgaa tggacaagtt gagtttcttg aggaggacgc ataatcgtgg aaaaagaaac
    24421 acaaaatgga aaagctgcgg ttttagggct gcagcatctg ctagcgatgt atgcaggagc
    24481 agtagcggtg cctcttttga tcggtacagg actaggcttt aatcaagaac agatgactta
    24541 tttgatttca attgatatct ttatgtgtgg attagcgaca ttattgcaat tgacagttaa
    24601 ccgatttttc ggaattggct tgcctgtcgt tttaggatgt gcgatccaag cagtagctcc
    24661 actgatcttg atcggaacca atgaaggagt aggagctatc tacggatcta ttatcgcttc
    24721 tgggatcttt gttgtgttag tttctggtgt cttttctaaa attaaaaaac tattccctcc
    24781 tatagtaaca ggaacggtta tcaccgttat tggtttgact ttgattcctg tagcaatcga
    24841 gaaaatgggt ggaggtaatg caacagcagc tgattttggt gacaaaacaa atctgctact
    24901 tgcatttgtg acgattctcc taattgtagg cgtgcaaatg ctggcaaaag ggttcattcg
    24961 ttcaatcgcg gtattgattg gtttagtcgg aggaagcatc ttagctgctt tcatgggaat
    25021 gattgatctg agcgcgattg gagatgcgcc gttgttccat gttccacaac ccttttattt
    25081 tggtaaacca actttcgacg tatggtcgat tttactaatg atcatcattt ccatggtcag
    25141 catggtagaa tcaacaggtg tatattttgc acttggtgac attactggga aaaatgttgg
    25201 agaagaagaa ttgaaaaagg gatatcgtgc agaaggactt gccgttatct taggcggtat
    25261 tttcaataca ttcccttata ctggcttttc tcaaaatgtc ggattggttc aactttcggg
    25321 aatcaaaaca agaaaaccca tctatttctc tgcattcttc ttgattgttt taggattatt
    25381 ccctaaaatc ggtgcgttag cacaaatcat ccctgagccg gtacttggtg gaggaatgtt
    25441 agtcatgttc gggatggtgg cagtccaagg aatccgtatg ctgttggaag ttgatttcag
    25501 caatgataaa aatctattga ttgttgctgt ttctgttgga tttgggctgg gtttcaatgt
    25561 tacgccgaca ttattcagtc aaatgccgca aacattgcaa atgttcactg gaaatggcat
    25621 cgtaatgagt agcattacgg ctatcatatt gaatttgatc tttaatggat tgaaaaaaga
    25681 tgaagaaata ttggaagaag ccttcaagaa gtaatagagc aaaagaaagg ttgtgaaatc
    25741 agcgttttga cctgattgtt agagaaacaa acggaaaaat agttcctcgt attttgccgt
    25801 ttgtttgagc taataccgag ggtcagctga ttgaacaccg ttgaaggaag aggtcgtgag
    25861 cctgccgttc atctccgagt cacaaggaac aatttcaaaa aaaagcttct catgtttgtt
    25921 gaatattgtt ccttgtgacc gaggagttgg caggagaaca ccgtttatcc ataaaaagga
    25981 atgtgaccta acttttgtca cattcctttt ttttatcttt aataaacacg aacaataaaa
    26041 tgtttttctt tgttaacatt cgaaatttct ttgacatcaa aaaaagtgat tgttaaaata
    26101 tgaatgtaga aaaactggga ggaaaaacaa tgacagcaaa aatctctgtg attatgggga
    26161 gtacttcaga ttgggaaaca atgaaagaag catgtcaaat attggatgaa ttcggtgtgt
    26221 cttatgaaaa aaaggtcgtt tctgctcatc gaaccccaga tttgatgttt gaatatgcac
    26281 aaaacgctcg aaaaaaagga atcaaaatca ttattgcagg agcaggggga gccgcgcatc
    26341 tgccgggtat ggtcgcatca aaaacaactt tgccggttat cggtgtaccg gtccaatcac
    26401 gaacattaaa tggtttggat tcgcttcttt cgattgtcca aatgcctgga ggagtacctg
    26461 tagctactac agcaatcgga aaagcgggag caattaatgc tggactactt gcgatacaga
    26521 tgctttccat gtatgattta gaattggagg ctaaattggc tgaaagacgt tcaatgttgg
    26581 ctaaaactgt tatagaaagt agtgatcaac ttggttaaac ctttgttgcc tggttcaacg
    26641 atcgggatcg tcggtggcgg tcaattaggt cgtatgatgg cgattagtgc aaaagaaatg
    26701 ggctttaaag taggtgtgct ggatcctgtg agcgattgtc cagccgcaca agttgcagat
    26761 tggcatattg aaagtgtcta tgatgacacc tttgcgctga aagagcttgc cagaagaaca
    26821 gatgtcatta cctatgaatt cgaaaacgtc agtgtagagg cgttaaatgc gattttaccc
    26881 atgtcattta ttcctcaagg gacagacttg cttgcgatta cgcaagaccg cctgttagaa
    26941 aaatcttttt tggaaacgaa taacatcgtg attgctcctt atgcaacgat cgtcagtccg
    27001 acggatatcc aagatgcgat tgacgggatc ggctatcctt gtgttttgaa aaccactcga
    27061 ggcggctatg atggcaaggg gcagtatgtt ttgaaaagtc gggcggatct agctcctgcc
    27121 atggaccttt taagagaagg aacctgtgtt ttagaagcat ggatcccgtt tgaaaaagag
    27181 atttcgatca tggtggcagg aaatggtcaa ggagatttca cgacttttcc ggtcgtggaa
    27241 aacatccacc acaacaatat tcttcatgaa acgattgctc cagccgcgat tgatcaggat
    27301 gtgatcgagg aggctgagcg tattgcccgt gtgattgcag aagcagtgag cctttcaggt
    27361 gttttagggg tagaaatgtt tttaacaaaa acaggcgggc tatatgtgaa tgaattagct
    27421 cctcgtcctc acaattctgg acattattct attgaagctt gttctatgag tcagttcgat
    27481 gcccatattc gaggtatttg tggttggcca ttaggagaaa aagtacgttt gataagcaat
    27541 gcagtaatgg tcaatgtttt gggagaacaa ctgctggaaa gctatcgctt gattgagaat
    27601 aagccagact ggcaattcca tttctatgga aaaaaacaag caaaaaaagg tcgcaagatg
    27661 ggacatatca ctatcttgac agaagacatc cagcaaacgc tggaagaaat caaacaaacg
    27721 aatatttggg attagaggag aaaaaacatg ttagaacgtt atacacgccc tgaaatgggg
    27781 aatatctgga cggaagagaa caaataccgt gcgtggctcg aagtcgagat tttagcagat
    27841 gaagcttggg cagaacttgg tgaaatcccg caagaagatg tgaaaaaaat tcgtgagcat
    27901 gcatcatttg atatcgatcg gatcttggaa atcgaaaaat taacacgcca tgatgtcgtt
    27961 gcgtttacac gagcggtttc tgaaacattg ggagaagaaa gaaaatgggt ccattatggt
    28021 ctaacaagta cggatgtcgt agatactgct tatggctatc tcatcaagca agcaaatgat
    28081 attttacggg aagacttgaa acgttttacg gaaatcattg gcgaaaaagc aaaagaacac
    28141 aaatatacag tgatgatggg tcggacacac ggtgtccatg ctgaaccaac tacttttgga
    28201 ttgaaacttg ccacatggta ttcagaaatg aaacggaaca tcgaacgatt tgaacatgcg
    28261 gcaaaaggag tagaagcagg aaaaatctca ggagcagtgg gaacgttcgc aaatatcccg
    28321 ccatttattg aagagtatgt atgtaaacaa ttggggatcc gtccacaaga aatttctact
    28381 caagttctcc ctcgggatct acatgctgaa tatttctcag cgatggcgct tattgctaca
    28441 agtattgaac gttttgcaac agagatacgc ggattgcaaa aatccgaaac acgtgaagta
    28501 gaagaatttt ttgcgaaagg tcaaaaaggt tcttctgcta tgccgcacaa aaggaatccg
    28561 atcggttcag aaaatatggc tggtctagcc agagtggtgc gcggacacat ggtcactgcc
    28621 tatgaaaatg tcacattatg gcatgaaaga gatatttctc attcttcagc agaacgaatc
    28681 atcttgcctg ataccacgat tttattgaat tacatgttaa atcgttttgg caatatcgtt
    28741 aaaaatctaa cggtctttcc tgaaaacatg aagcggaata tgtctgcaac atttggtttg
    28801 atttatagcc agcgcgttat gctgaaattg atcgataagg gaatgacaag agaagaagct
    28861 tatgatcttg ttcagccaaa aactgcgtac gcttgggaca accaagtgca gttccgcccg
    28921 ctgttagaag aagatccaac catcacatcg gtattatcta aagaagaaat agacgatgcc
    28981 tttgattatc atcatcattt gaaaaacgtt gacgtgatat tcaaacgagt aggtttggaa
    29041 taaagaagag tgagttgcaa aattcaatta aaatagaata gcaaaaataa ccaaaaaaga
    29101 agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat caataatagg
    29161 aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa aaaatagagg
    29221 aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag atgttgcagc
    29281 aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg gatacgataa
    29341 tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc tttgtgttct
    29401 gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc tcggcggatt
    29461 tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt gatcgacagc
    29521 aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa aagctgacct
    29581 tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca aatttttagt
    29641 taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt ataagatttt
    29701 cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac atatttttgt
    29761 tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa aaacgatgta
    29821 tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa cttttgtatg
    29881 aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg atagaatatt
    29941 tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga aaaggagaat
    30001 tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga ataaaaaatc
    30061 attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa attatccctc
    30121 tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta ggtgttcccg
    30181 aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac cagttggatg
    30241 atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg gaagaaatca
    30301 gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta tttgctaagg
    30361 tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga caaattttgc
    30421 ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg aatgaacata
    30481 tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat caagaagtat
    30541 ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt tcgtaaaagt
    30601 atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa aaaaagcagt
    30661 ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact ttgagataca
    30721 acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg acaagctgct
    30781 tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg aagtataaca
    30841 tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg ttatgggcag
    30901 ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc tctttagaag
    30961 gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg agatgcggtg
    31021 cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa gaagggaaac
    31081 cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt ttgccaggtg
    31141 tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg aaagtcaacc
    31201 ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt ccagttgctc
    31261 atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa aaaaataatc
    31321 agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt gcagggatta
    31381 cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca gtagaagact
    31441 tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac ttcggaaagg
    31501 tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag aaaaaaagaa
    31561 tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa gatatcttag
    31621 gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc gaacattgtt
    31681 cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct catgtgttgc
    31741 aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca gttgtcttca
    31801 aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct gcgacaggag
    31861 ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg ttgctagatt
    31921 cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa gaagtagttg
    31981 caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc gaaattgcat
    32041 ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt ttgattgaac
    32101 acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg tatgtaggtg
    32161 caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa tttaatcaag
    32221 aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa aagctgctgc
    32281 ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt atacaggata
    32341 tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct ggctcaggac
    32401 ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct tatgaaatga
    32461 tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag gaagaaaaaa
    32521 tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa gtaacagacg
    32581 acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca gtcgatgcat
    32641 tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga atcgtagaat
    32701 ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta ttgcttgatc
    32761 tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat gattcacaag
    32821 tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg gttagaggga
    32881 cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat ttggatccaa
    32941 aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct agcggtggta
    33001 agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca gaagtttttt
    33061 gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta ggaactcctg
    33121 ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc tatccaacac
    33181 caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact caggaattca
    33241 aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt gatggcagtg
    33301 aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt gatctatcca
    33361 ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg attgaaagtg
    33421 ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct ttcaaacatc
    33481 agctagggat atctgtacag tttgaattgt cctctgctca attatttgca gaaactcaat
    33541 caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa atgatgggag
    33601 atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt tcagcaactg
    33661 acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat gcaatcgtat
    33721 gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt ggggtcatcc
    33781 agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag gacaagaagg
    33841 tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt tggggctgct
    33901 ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag ctgcaatcgg
    33961 acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc cttttctatt
    34021 caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca atgctcgttc
    34081 ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg atacggagat
    34141 attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat taaaaagtgc
    34201 tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg caatgattgc
    34261 cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa acggagcata
    34321 cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta taagagatgt
    34381 ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt gctttacgaa
    34441 ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac cagattcaaa
    34501 catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt tagcgaaaga
    34561 atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt cagccgcaag
    34621 cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa atcaatacat
    34681 tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc gaatgaaatt
    34741 atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg attcaattgt
    34801 tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg caaaagaagt
    34861 ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga ttgatattca
    34921 aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag aatgtatcgg
    34981 tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg gtctggaaaa
    35041 agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc cgacgccgtt
    35101 atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt tcttttaaaa
    35161 gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa gctggcgtag
    35221 atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa aaaacggaac
    35281 gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta tcggtctttc
    35341 aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa ttagcagtgg
    35401 caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg tgtgtcaatg
    35461 atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca acggggaaaa
    35521 atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt gttcaaggaa
    35581 atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca ggtaatgatt
    35641 atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg acaggagaaa
    35701 cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat tcaaatgggt
    35761 actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca tcaaaactgc
    35821 cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc tatgtttctt
    35881 ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc actggtggag
    35941 ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa atcaaacttg
    36001 gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa attcctgaga
    36061 tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc gaagaatcga
    36121 aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat gaaatcggga
    36181 aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga tgagaatcgc
    36241 tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc tatcgaaaaa
    36301 aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat atgttttgaa
    36361 acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat ttgattcaaa
    36421 aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg atttgatcgt
    36481 attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg ataaaagaat
    36541 catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca tacgtgatgc
    36601 ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc aaggtgtaga
    36661 tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca cgttcgattc
    36721 tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga ttagtgaaat
    36781 tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc agataaaact
    36841 gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat ttcaacagga
    36901 ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga agaagtgaca
    36961 gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat ccatggtggt
    37021 cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga aaatatccaa
    37081 ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat gaaaccagat
    37141 gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat gctccgtagt
    37201 gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta tgaagaagta
    37261 ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa actagcggca
    37321 aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt aacggattgg
    37381 gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca aacgcttcgt
    37441 tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct ggaaaaatat
    37501 tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa tatccgagat
    37561 gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt cgccttgaaa
    37621 cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg ggaagcagcg
    37681 tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg agaagtagat
    37741 ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc cccttcttat
    37801 acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact gacagtagac
    37861 tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg tggacttctt
    37921 gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt gacaaaacgt
    37981 aagccgactc cagcagaagc aaaagcatta gtgtttgctt ggagagctgt caaacatgtg
    38041 aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg agccgggcag
    38101 atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa aatggaaggt
    38161 gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata tgcagccaaa
    38221 catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga ttctattgat
    38281 atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt tagacattag
    38341 agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga acatgcaata
    38401 agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc aggaaatccg
    38461 gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca ttcagcgtta
    38521 atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga agttcctttg
    38581 ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg tccgacaaaa
    38641 gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga tacttatcaa
    38701 atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc gtatatcaaa
    38761 gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa aggtgtagtc
    38821 gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa ccagcatcga
    38881 tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga agaattctcc
    38941 cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca agatcataag
    39001 cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta ttcaccagta
    39061 ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa accagcggct
    39121 ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg tttgattgct
    39181 acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc tgaaacgcaa
    39241 gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt attagagaat
    39301 cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt ggttgcagct
    39361 gccggttacc ccgaagaata tgagacagga atggttctac cagattttct ggaagaagag
    39421 ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg agcaggagga
    39481 cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa aaaaatttac
    39541 accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat tggagaaaaa
    39601 gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata aaaaatacaa
    39661 caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg tggtacactt
    39721 ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag gtacttctga
    39781 tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg aagatccagt
    39841 aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg cagatattta
    39901 tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat caatcaaacg
    39961 agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt ttgatttttc
    40021 taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa tggattatgt
    40081 ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag tagctgaagc
    40141 gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca atcaaaaacc
    40201 aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta atcagctgca
    40261 atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata tgacagatga
    40321 agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg atatgacgat
    40381 ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg ggaacgaaaa
    40441 attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa cgacaccaac
    40501 tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga tcgccggtac
    40561 gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata tgtctcggga
    40621 agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat tgtcataagc
    40681 aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt tcagcttcga
    40741 gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat ttcgtttatt
    40801 agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa atcccttgtt
    40861 ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca aaagcaatat
    40921 ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt ctttgttctg
    40981 tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg aaatgagagg
    41041 gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg gtaaaatttt
    41101 ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt gaaggctgtg
    41161 agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa agcgagtcgt
    41221 cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct tgcgggattt
    41281 tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa cgatataatc
    41341 gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta gatacttcgt
    41401 ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa tcaactggaa
    41461 gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg gaactggcgg
    41521 tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt ctttctgcga
    41581 caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca tggaccaacg
    41641 tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca actattaaaa
    41701 atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc ctaaataaaa
    41761 acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact tggtttgctg
    41821 ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca gcaagtttca
    41881 atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga ttttttttca
    41941 attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct aaagcagtta
    42001 ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc agataatcat
    42061 ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca ctaataaata
    42121 tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac ccgtccatga
    42181 aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt gaagacagtg
    42241 ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa ccaaggacag
    42301 ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata aaaactttat
    42361 acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt gacagttgtt
    42421 ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta ataaagaagg
    42481 agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc gatccctttg
    42541 gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc gttgtctata
    42601 actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa tcctttgtga
    42661 cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa gatcattttt
    42721 aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag catatggccg
    42781 tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt ttcaagagga
    42841 atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc atgggtcata
    42901 gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc caaactgtta
    42961 taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg tgctaaatgg
    43021 tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat tttattcatg
    43081 ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa gccaaggttc
    43141 cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg gacctttctc
    43201 tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac tccaagttcc
    43261 actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct tgctaagtcg
    43321 atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac agaatctacc
    43381 tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt attgatcagc
    43441 tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa cgtactaatt
    43501 tctttaagtg tatttgtatt atttttgctc tctcgaatat cattcctaag ttcttctagg
    43561 atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc ccacatactt
    43621 tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat atttgcgatc
    43681 gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag caaagggata
    43741 acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat gcttaaattt
    43801 tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta tattctgctg
    43861 ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg tttgaatatt
    43921 tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt tacactcgac
    43981 tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc catcaatagt
    44041 tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag ctaaagcacc
    44101 gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat catttgtttc
    44161 taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga caattcggtg
    44221 aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg ttggtttacg
    44281 attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt tctctgaacc
    44341 agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat tgctgacaaa
    44401 taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga cgaaaagctc
    44461 gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg ctcgctttgc
    44521 ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga tcatatccac
    44581 tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca tgtagccatt
    44641 tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt cagtatgctt
    44701 tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg ggtcaggtgc
    44761 ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa gggctaaaaa
    44821 aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact atcttatcag
    44881 ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga aacttaaaaa
    44941 aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa aaaaatcaat
    45001 gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg ataaaaaatg
    45061 aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag cattctatta
    45121 agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg aatcgctatg
    45181 ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc ggaattagaa
    45241 aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa tcaagtagca
    45301 caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc tatcgatggt
    45361 tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt gatcgcctat
    45421 gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga taattttgga
    45481 gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga aggaaaagga
    45541 ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact aatcaattac
    45601 aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt cgtgccatct
    45661 ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac ggcgcaagca
    45721 aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc tgttttgtcg
    45781 ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg gtatgggagt
    45841 gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg cgttaaatcg
    45901 atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt agcaaaaaat
    45961 acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa tgatactgaa
    46021 acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt atctgtcgat
    46081 aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc cgatctgaaa
    46141 aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg ataagaatta
    46201 tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga atccgagcgt
    46261 tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc ggagaaaacg
    46321 gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat ggctcctatg
    46381 acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa gatagtgaag
    46441 aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt tcaataaaag
    46501 agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg gaactgacag
    46561 aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg aatcttctag
    46621 tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca ttatcaaaat
    46681 ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa gaaagcgcga
    46741 atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc attatttcac
    46801 ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg gatggacaga
    46861 cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc ggaatggtgg
    46921 gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa gtttatttta
    46981 aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata aacgaccaca
    47041 tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg ggagcgggca
    47101 gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata tccggacgta
    47161 tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt gcaaacggac
    47221 tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg gatattcggg
    47281 aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta gataaagaaa
    47341 aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca aactccatcc
    47401 atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta tccaagtggc
    47461 tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata gatgttgggg
    47521 caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa gctgtctgtc
    47581 ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat acgatgaacg
    47641 aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc ttgatgaaat
    47701 taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac ggataaaaaa
    47761 gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta cagcatggtg
    47821 atcattttag cgggactact tattgcgttt caattgatga cggatggtat tttctggcgt
    47881 ccattgaata taactaacct tgttctgcaa aatagccata tattagtact agctgcaggg
    47941 atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt gatggcgttt
    48001 gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg gatgacagtt
    48061 atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg ggtagcttat
    48121 gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg tggactgaca
    48181 caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca aaaaatgtct
    48241 acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac attgatttta
    48301 ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa tcgtcggaaa
    48361 aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt atttacagca
    48421 gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc gattattttg
    48481 atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac agtagcaggt
    48541 cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg gatcaaaaca
    48601 aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt agctggatta
    48661 gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat ggaactggat
    48721 gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg aacaatcatg
    48781 ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc gattttaggc
    48841 gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc cgttgtgcta
    48901 gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc agaaaaaaga
    48961 ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc attgtttcgt
    49021 ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct ctttttgctc
    49081 ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc cttgattaaa
    49141 ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa tgaaagtatt
    49201 tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc gttctgaatg
    49261 tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc ctgccaacat
    49321 gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt atttctatat
    49381 catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga aaaagcgtgg
    49441 attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta tagagaagtt
    49501 ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg gacatgctaa
    49561 ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt ttgtgattgc
    49621 cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg cagatgcaac
    49681 aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag gctttgggac
    49741 tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca agccgatcat
    49801 cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt ttggtgcgac
    49861 aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg aaacaaaagt
    49921 cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc aaaaaggcga
    49981 aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt tgaaagatac
    50041 actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag gaagagatct
    50101 ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta atggagacac
    50161 aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc attaagtaat
    50221 aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat tattatcgca
    50281 gatacaagct tttgaacatc attattcaga ataagggact gtgataagat ttttgtcaca
    50341 gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt aaaatcaaaa
    50401 gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa aaaacaagga
    50461 ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg aaggatcaat
    50521 cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt gaatcgttcc
    50581 accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag caaacgtggc
    50641 agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag aatcgattgg
    50701 cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa aataagagaa
    50761 gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat gccagatgag
    50821 cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc ggaagaaaaa
    50881 gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta tttggaaagt
    50941 aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg tcagcaagca
    51001 atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt agagtcacct
    51061 tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt tgggataccg
    51121 atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa aaataaattg
    51181 aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat gagtcaaaaa
    51241 cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat tgaagacgat
    51301 gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg aacgattgat
    51361 ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac aacgaaaatc
    51421 ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca agaaatgatg
    51481 gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa tgaagcaatt
    51541 gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact gataagcgct
    51601 tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta ctatgcctgg
    51661 attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt agatatgggt
    51721 cttggaattg caccaagtct actttttggg caagatataa atggaatcag aatcaattac
    51781 agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat tctagaaaag
    51841 tggttaacag aataatttca caaagtaaat agattaaaga atataacagc ttggatttaa
    51901 atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac taagaaaaaa
    51961 ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca acaaaactgt
    52021 ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga aaatagaaaa
    52081 aatgcagatg aaatataaaa cgctttcctt attgtgattt tattcactaa tgctgttaat
    52141 atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct tgtatttctc
    52201 ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt ttattcactg
    52261 tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt gacaaatggc
    52321 aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg cagatttccc
    52381 agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag tacctgattt
    52441 atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg tattagacca
    52501 acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa ctgctggaca
    52561 agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc ttcttccagg
    52621 ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag cttttttatg
    52681 gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc taccaccaca
    52741 aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt tgaagaaacg
    52801 aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac aaggtgattt
    52861 ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt atatccaaaa
    52921 caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa cacttgcaca
    52981 aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc cgcttgcagt
    53041 atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac cagtcctaat
    53101 cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc caacccgcta
    53161 ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc gcttccgtaa
    53221 atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca tcgaagcaac
    53281 taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac aaaaggtttc
    53341 agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac aaattgcaat
    53401 ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca agcaatcaca
    53461 gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg tgaagatgtt
    53521 gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt cggagaagac
    53581 cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt cggtcttgcg
    53641 ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt cgaagcaatg
    53701 gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac tagaaatcta
    53761 cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt gcattcggat
    53821 aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc ttccaaccct
    53881 tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt tgttttccta
    53941 gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc ttacgaagtt
    54001 cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat cacatacgga
    54061 gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa tatatctgtt
    54121 gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat tcaatccgtg
    54181 gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg cgttgccgcg
    54241 caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc aatcggacga
    54301 gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg gttgccaaat
    54361 gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa gaagggacga
    54421 aacaaagtca tggcttatca atttaaatt
//
LOCUS       DACEDO010000011        49253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEDO010000011 DACEDO010000000
VERSION     DACEDO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.011"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            257..532
                     /locus_tag="IWO52_000708"
     CDS             257..532
                     /locus_tag="IWO52_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0354996.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="IWO52_000709"
     CDS             545..2623
                     /locus_tag="IWO52_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0354997.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="IWO52_000710"
     CDS             2783..4669
                     /locus_tag="IWO52_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0354998.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="IWO52_000711"
     CDS             4681..5628
                     /locus_tag="IWO52_000711"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0354999.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="IWO52_000712"
     CDS             5648..6175
                     /locus_tag="IWO52_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0355000.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="IWO52_000713"
     CDS             complement(6238..6891)
                     /locus_tag="IWO52_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0355001.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="IWO52_000714"
     CDS             7023..7865
                     /locus_tag="IWO52_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0355002.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="IWO52_000715"
     CDS             8023..8919
                     /locus_tag="IWO52_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0355003.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="IWO52_000716"
     CDS             8922..9635
                     /locus_tag="IWO52_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0355004.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="IWO52_000717"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="IWO52_000717"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0355005.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="IWO52_000718"
     CDS             10170..10397
                     /locus_tag="IWO52_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0355006.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="IWO52_000719"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="IWO52_000719"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0355007.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11725)
                     /locus_tag="IWO52_000720"
     CDS             complement(11183..11725)
                     /locus_tag="IWO52_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0355008.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="IWO52_000721"
     CDS             complement(11962..12360)
                     /locus_tag="IWO52_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0355009.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="IWO52_000722"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="IWO52_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0355010.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="IWO52_000723"
     CDS             13473..14240
                     /locus_tag="IWO52_000723"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0355011.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="IWO52_000724"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="IWO52_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0355012.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="IWO52_000725"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="IWO52_000725"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0355013.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="IWO52_000726"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="IWO52_000726"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0355014.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="IWO52_000727"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="IWO52_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0355015.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="IWO52_000728"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="IWO52_000728"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0355016.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="IWO52_000729"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="IWO52_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0355017.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="IWO52_000730"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="IWO52_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0355018.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="IWO52_000731"
     CDS             22691..23566
                     /locus_tag="IWO52_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0355019.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23698..24336)
                     /gene="plsY"
                     /locus_tag="IWO52_000732"
     CDS             complement(23698..24336)
                     /gene="plsY"
                     /locus_tag="IWO52_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0355020.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24463..24897
                     /locus_tag="IWO52_000733"
     CDS             24463..24897
                     /locus_tag="IWO52_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0355021.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25063..27108
                     /gene="parE"
                     /locus_tag="IWO52_000734"
     CDS             25063..27108
                     /gene="parE"
                     /locus_tag="IWO52_000734"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0355022.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27120..29570
                     /gene="parC"
                     /locus_tag="IWO52_000735"
     CDS             27120..29570
                     /gene="parC"
                     /locus_tag="IWO52_000735"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0355023.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29847..32078
                     /gene="pflB"
                     /locus_tag="IWO52_000736"
     CDS             29847..32078
                     /gene="pflB"
                     /locus_tag="IWO52_000736"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0355024.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32322..33083
                     /gene="pflA"
                     /locus_tag="IWO52_000737"
     CDS             32322..33083
                     /gene="pflA"
                     /locus_tag="IWO52_000737"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0355025.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33168..34094
                     /locus_tag="IWO52_000738"
     CDS             33168..34094
                     /locus_tag="IWO52_000738"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0355026.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34276..35169
                     /locus_tag="IWO52_000739"
     CDS             34276..35169
                     /locus_tag="IWO52_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0355027.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35303..35428
                     /locus_tag="IWO52_000740"
     CDS             35303..35428
                     /locus_tag="IWO52_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0355028.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(35562..36194)
                     /locus_tag="IWO52_000741"
     CDS             complement(35562..36194)
                     /locus_tag="IWO52_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355029.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(36342..36884)
                     /locus_tag="IWO52_000742"
     CDS             complement(36342..36884)
                     /locus_tag="IWO52_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0355030.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            37019..37408
                     /locus_tag="IWO52_000743"
     CDS             37019..37408
                     /locus_tag="IWO52_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355031.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            37412..38176
                     /locus_tag="IWO52_000744"
     CDS             37412..38176
                     /locus_tag="IWO52_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0355032.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            38305..39252
                     /locus_tag="IWO52_000745"
     CDS             38305..39252
                     /locus_tag="IWO52_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0355033.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            39382..40122
                     /locus_tag="IWO52_000746"
     CDS             39382..40122
                     /locus_tag="IWO52_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355034.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(40413..41081)
                     /locus_tag="IWO52_000747"
     CDS             complement(40413..41081)
                     /locus_tag="IWO52_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0355035.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            41215..41502
                     /locus_tag="IWO52_000748"
     CDS             41215..41502
                     /locus_tag="IWO52_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355036.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            41918..43456
                     /locus_tag="IWO52_000749"
     CDS             41918..43456
                     /locus_tag="IWO52_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0355037.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            43453..44418
                     /locus_tag="IWO52_000750"
     CDS             43453..44418
                     /locus_tag="IWO52_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355038.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            44405..45727
                     /locus_tag="IWO52_000751"
     CDS             44405..45727
                     /locus_tag="IWO52_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0355039.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      45790..45894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            45968..46711
                     /locus_tag="IWO52_000752"
     CDS             45968..46711
                     /locus_tag="IWO52_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355040.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            46726..47718
                     /locus_tag="IWO52_000753"
     CDS             46726..47718
                     /locus_tag="IWO52_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0355041.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            47730..48467
                     /locus_tag="IWO52_000754"
     CDS             47730..48467
                     /locus_tag="IWO52_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355042.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            48457..49179
                     /locus_tag="IWO52_000755"
     CDS             48457..49179
                     /locus_tag="IWO52_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0355043.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT        17056 a         8418 c        10306 g        13473 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagactatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcgatttg tcacactccc gttttatctc tttggaatga aaaagtatca
    23701 ggattttttc ttgctgctat tcaaaccaaa cgggattcga ctttcagtat ggtttctgat
    23761 acgcttgata ttgtctttat gccgataaaa gataaaggaa gaaactaaaa aggcaatgat
    23821 cgtcagtaac caattgggct tagccaaaat cacaggagct acataaggaa ggacgatcgt
    23881 agataaagtg atcaatactg cactgatcat actgctcaaa ctcaccatac ttgtacaaaa
    23941 taaactaacg ataaagatga tacaagaata aacaaaaaat acaggattaa atcctaaaag
    24001 cattcctgca cttgtcgcaa cagcttttcc tcctttgaac ccagcaaaaa tcggaaatgt
    24061 atggccaaga atggcgcata ccccaaacca aagagggtta atcccagaaa ggtggaagat
    24121 catcggcaaa caagtagcca atgtaccttt taaaatatcc ataagcagga caacaatccc
    24181 ggctgttttg cctaatacac gaaatgtatt ggtcgttcca gtattaccgc tcccgtgttg
    24241 tcggatatct tttttgaaaa acaatttgcc aatccagaca ccggaaggaa tagaacctaa
    24301 aagatatgct aaaataagta ataagatcaa tttcattttt taccctcttt caagttgaaa
    24361 actatccttt attttagcat gaataggtaa caggaacaat tgaaaaaatg aaagatacaa
    24421 gtataataga tggaataaaa agaaaagcaa ggggtggcag aaatgtctgt aaaaaatcca
    24481 acacaagaaa cgattttcaa ctatttggaa gaagcaaaaa atattgccgt cgtaggcttg
    24541 agcaataaac cagaacgaac aagttacatg gttgcacagg tattgcaaga caatggatat
    24601 aaaatcatac cagtaaatcc attattggct ggtgaagaaa ttctaggcga gaaagtctat
    24661 gcgaatttga cagaaatcga tgatccaatc gatatcgtag atgtatttag acggagtgaa
    24721 tttttacctg aaatagcagg tgattttttg aaaactgatg ctaaagtgtt ctgggcacaa
    24781 ttagggattg aaaacaaaga agcagctgag atgctaagaa aagcaggacg tacagatatc
    24841 gtaatggatc gttgtattaa gattgaactt ggtaagttga aagaaaacca aaaataaaaa
    24901 tgaccacaaa gaagagatga tcgatagagg ggaatgtcga aaaagcggtt aggagataat
    24961 taaaattgat ttctcgtcta ccctcttttt tttcacgacc ttttctagta tgataatcgt
    25021 ggtgcggttt tgccgcaaag tattattgaa ggagcgtttg ccttggcaaa aaaagtaaac
    25081 aacgaataca atgactcgtc catccaagta ttggaaggat tagaggcagt tagaaaaaga
    25141 ccagggatgt acattggatc cactgacagc cgaggcttgc atcatttagt ctatgaaatc
    25201 gtcgataatg cagttgacga agcattatca ggttatggca gtgagattga tgtgaccatc
    25261 catgaagaca atagtataac tgttgcagat agcggacgtg ggatgcctgt aggaatgcat
    25321 gcttccggaa taccaacagt tgaagtgatt tttactgtgc tgcatgctgg cggaaagttt
    25381 ggtcaaggag ggtacaaaac ttctggtgga ctgcacggtg taggagccag tgtggtaaat
    25441 gccttatcga aatggctaat tgtaacgatc gttagagatg gcgtagagta tcagcagaaa
    25501 tttaaaaacg gcggtaaacc tgacggtact ttgaaaaaga taggaaaaac aaaaaaagca
    25561 aacgggacga ctgtccattt tttaccagat gatacaatat tcagtacaac gaaattttca
    25621 tatgaaattt tagccgaacg gttaagagaa tctgcctttc ttctaaaagg cgttaaaatc
    25681 tctctttctg atcttcgtgg agaagaacca gtcaaggaaa tttttcatta cgaagaagga
    25741 atcaaagaat ttgttgatta tctgaatgaa gaaaaagata cattgacacc agttgtttat
    25801 ttttcaggag aaaaagaagg tatcgaagta gaggtcgctt atcaatacaa tgacggttat
    25861 tcagaaaatg ttttatcttt tgtcaataac gttcggacaa aagatggggg aacccatgaa
    25921 gctggtatga aagcggcgat gacgaaatct tataatgaat atgcgagaaa agttggtttg
    25981 ctgaaagaac gggacaagaa tctggaaggt agtgatttca gagaagggtt agctgctgta
    26041 ctttctatcc gtgtacctga aaatcttctg caatttgaag ggcaaacaaa agaaaaactt
    26101 ggtacgccag ttgctcgtac cgtagtagat aacgtcatca gtgaacaaat gggcttttat
    26161 ttgcaggaaa atagcgaaat gagccagatg ttggtccgta aagcaatcaa agccagagaa
    26221 gcgagagaag cagcacgtaa agccagagaa gaaagccgaa atgggaagaa gcgtaaaaaa
    26281 ggcgaatctc ttctttcagg aaaattgaca cccgcacaat cccgaaaccc taagaaaaat
    26341 gaattgtatt tagtcgaagg ggattctgct ggcggatctg ctaaacaagg aagagacaga
    26401 aagttccaag caattctacc tttacgagga aaagtcatca ataccgaaaa agctaaaatg
    26461 caagatatct tgaaaaatga agaaatcaat acgatgatct atacgattgg tgcaggagtc
    26521 ggtcctgagt tttctatcga agactgtaat tacgacaaag taatcatcat gaccgatgcg
    26581 gatacagacg gcgcccatat tcaagtatta ctgcttactt tcttttatcg ttatatgaaa
    26641 ccgctgattg aagctgggaa agtatatatc gcgttaccgc ctttatataa agtatccaaa
    26701 ggacaaggaa agaagcaagt cattgaatat gcgtggacag atgacgaatt ggcagcaatg
    26761 attaaaaaag tcggcaaagg atatatgctg cagcgatata aaggacttgg agagatgaat
    26821 gcggaacaat tgtgggaaac aacgatggat ccgacttccc gtacgttgat ccgcgtccgt
    26881 atcgatgatg ctgcgcaagc tgaacgtcga gtaaccacac tgatgggtga caaagtagaa
    26941 cctcgaagaa aatggatcga aaatcacgtg caatttacgc tcgaagaaga tggcagtatc
    27001 ttagataaaa aagaggatac tgaaatctct ccttcagttt ctaatgattt attagatgaa
    27061 gaacgagcag ataaaaatga aaacaatcag ttatttgaag tagaatagga ggtcacctga
    27121 tggaaaatcg acaagaagta caagaattaa ctttagaaga agtgatgggt gaccgcttcg
    27181 gacgttattc caaatatatt atccaagaac gtgcactgcc tgatatccgt gatggattga
    27241 agccagtgca acgccggata ttatttgcca tgaacaaaga tggcaatacg tatgacaaag
    27301 gattccgtaa atcagcaaaa tctgtcggaa atatcatggg gaattatcac cctcatggtg
    27361 accgcagtat ttatgaagcg atggtccgca tgagccaaga ctggaagttg cgggaagtac
    27421 tgatcgaaat gcacgggaat aacgggagta tggacggtga tccacctgct gcgatgcgtt
    27481 atactgaagc cagattatcc aagttaagtg gagagatgct agcagatatc gagaaagaaa
    27541 cagtcgatct cgtgtggaat tttgatgata cagaaaaaga accaacagtc cttccagctc
    27601 gatacccaaa ccttctcgtc aatggatcga caggtatatc tgctggatat gcaacagaaa
    27661 tcccgactca taacttagct gaagtgatcg atggcacaat ctatatgatc gatcatccac
    27721 aagcgagcct tgaaaaattg atggaatata ttcctggacc tgactttcca acaggaggga
    27781 tcttgcaagg aaaagatgaa atcaaaaaag cttatgaaac aggacgagga aaagttattc
    27841 ttcgttcgaa aacaaagata gaggcaatga agggtaataa acagcaaatc gtgatcacag
    27901 aaatcccata tgaagtaaat aaagcgacct tggtgaaaaa aatggatgag atccgtttga
    27961 ataagaaaat cgatggaata gctgaagtac gagatgaaag tgatagaaca ggtctgcaaa
    28021 ttgtcgtcga attgaaaaaa gacgccaatg ctcaaggaat tttgaattat ttgttcaaaa
    28081 acactgagct acagatcaat tacaatttca atatggtcgc tatcgaccat atgactccgc
    28141 atcaagttgg actgaaagac attttaagaa gttatatcga gcatcgaaag caagtcataa
    28201 caaaacgtag tcaattcgat ttagccaaag ctcaaaaaag acagcatatc gtagaaggat
    28261 tgatgaaagc tttatcgatt ctggatgaag taatcgcaac gattcgtgaa agtaaagaca
    28321 aaaaagacgc caaaaagaat ttagtcgacg tctttcaatt tacagaagaa caagcagaag
    28381 caattgtcac attgcaatta taccgtttga cgaacacgga tatcacagag cttcaaaagg
    28441 aatccgaaag cttgattgcg caaatcacag agttgaataa aatcctttca aatgacaaag
    28501 agcttttctc tgttatgaaa aaagaattgc gagaagtgaa aaaaaattat tcttccgctc
    28561 gtctaacgac aatcgaagac gaaatcgaag aaatcaaaat tgatacgcaa gtcttagtgg
    28621 cacaagaaga cgtaattgtc agtgtgacgc gagaaggcta tgtgaagcga acaagccttc
    28681 gttcttatag tgcttccaaa ccagaagaaa tcgggatgcg agaaggcgac tacttgctat
    28741 actcaggtga attgagtaca ttggaccatg ttcttctgat cacaaataaa gcaaatgtga
    28801 tctaccgccc tgtccatgaa ctacccgatt tgaaatggaa agatgctggt gagcacattt
    28861 ctcaaacaat ctccaatctg tctgtagacg aatcgatttt agctgttttc ccatatcaaa
    28921 aaattcaagc agagaaaaca tttgtattca tcagcaagaa tggattgatc aagcaaacac
    28981 gaatgacaga gttcgaacca tggcgcacct ataagagccg tccgctcagc tgtatgaagc
    29041 taaaggcgga agaagatgaa ctgattgctg tgtatctgaa agaagatcag ccaggcttag
    29101 atgttttctt agtaacgcat caaggaatgg gtttgcgcta tcctttaaca gatgttcccg
    29161 ttgttggagc aaaggcagct ggagtgaagt ctatgaattt gaaagatgga gataggatta
    29221 tcaacgggct gcttgtgtta gcagaaggtg atacgccgat cgtaatcgtt actcagcgag
    29281 gagctgttaa acgcatgctt gctcaagaaa tcagccaaac ttcacgtgcg aagcggggag
    29341 tgacggtcat gcgtgagctg aaaaaaaatc cgcaccgtat tatctatatg tcggaaggaa
    29401 gatcaaaaga aataaccttg atgaatcaaa aagggcagca gttaacgatt gacccaactg
    29461 atttccctat aggtgatcga acatcgaatg gatcatttgc gatggatgag aaaaaaggtg
    29521 gagaagtcat cgaagttatc gatgctcctg agattttcat cgagtcgtaa ctgttgtagt
    29581 attttaatga agagacgcca tctgtattaa tacaggtggc gtctcttttt cattcatttt
    29641 taataaatcc ttatacgttt gcaaaaaaag atgaaagtgt tcacaatgct taaatgttgg
    29701 tatgtcaacg ttgatttaaa tatatgtaaa taaaacataa aaattgttaa ataaaaaaca
    29761 agaaaaccct tgcaaagata gggtgctgtg ttatactaat cacgtaaaaa ctgttacata
    29821 aaacagaaat ttaggaggtc catacaatgg agcaatggaa tggatttaaa ggtaccaaat
    29881 ggaaagaagg cgtagacact agagacttta ttcaaaataa ctacactgaa tatcgcggtg
    29941 atgacagctt tttggaaggc ccaacagaag caacagaaaa attatgggat gaattgcaaa
    30001 agttaaatga tatccaattc gagcgtaacg gtgtctatga tatggacaca gaaatcgtat
    30061 caactattac ttcacacgat gcaggctatt tggataaaga tttggaaaaa atcgtcggtc
    30121 tacagacaga caaacctttg aaacaagcat tcatgccgtt cggcggaatc aatatggcta
    30181 acaatgcgct aaaaagcaat ggctatacac caaacgatga attgacacat attttcactg
    30241 actggcgcaa aacacataat caaggtgtat ttgacgctta tactgctgaa atgcgtgctg
    30301 ctcgtaaaaa caaaatcatt acagggcttc cagacgctta cggtcgcgga cgtatcatcg
    30361 gtgactatcg tcgtgtggca ttgtatggaa tcgactattt gatggaacaa aagaaaaaag
    30421 atcatgatat gactggttat catacaatga gtgaagaagt gattcgttta cgtgaagaaa
    30481 tcactgaaca atatcgtgca ttagcgcaaa tgaaagaaat ggctgcgaaa tatggctatg
    30541 atatttctaa accagctgca aatgcaaaag aagctgtcca atggctatac ttcggatatt
    30601 tagcagcaat caaatcacaa aatggtgctg cgatgtcaat cggacgtatc tcagcattcc
    30661 ttgatatcta tatccaacgt gatttggata atggagtaat cacagaaaaa gaagcacaag
    30721 aattgatcga ccacttagta atgaaattac gtatggtcaa attcgcgcgt acacctgaat
    30781 ataaccaatt attctcagga ttccctatct gggctacttt atccattgca ggtatgggat
    30841 tagatggtcg ttcattagtt acaaagaatg atttccgtct gcttcataca ttaacaaata
    30901 tgggaccttc accagaacca aacttgactg ttttgtattc tgaacacctt ccagaaggat
    30961 tcagaacata tgctagcaga atcgctatcg aaagttcttc gattcaattc gaaaatgata
    31021 atcagcttcg tgaacaatgg ggttctgatg attgtgcgat tgcttgctgt gtatcaggaa
    31081 cagttgtcgg aaaagacatg caattctttg gcgctcgtgc taacttggct aaaacactat
    31141 tatatgccat caatggcgga gtcgatgaag tgactggcgc ccaagttgga ccagaatatc
    31201 gtccaatcac atcagaaata ctagactttg aagaagtgaa acataacttc atgaatatga
    31261 tggattggtt agcagaacta tatgtcaata cgttgaacat cattcattat atgcatgata
    31321 aatattctta tgaagcagct cagctggctt tgatggattc tgacttgcaa cgcacatttg
    31381 ctacaggtat tgctgggatc tctcatgcgg cagacagctt gtcagcgatc aaatatgcaa
    31441 aagtaaaagc aatccgtaat gaaaaaggaa tagcagttga ttttgaagtg gatggcgact
    31501 tccctaaata cggaaatgat gatgaccgtg cagatgaatt agtttcatgg atcttggaat
    31561 acttcatggg acaaatcaaa cgtcataaaa cttaccgcaa ctcaaaacca acaacttcat
    31621 tattgacgat tacttcaaat gttgtttacg gtaaaaatac aggaaatact cctgatggcc
    31681 gtgaagctgg aaaaccactt gcgccaggag caaatccaag ttacaatgcg gaacaaagtg
    31741 ggttattagc tagcctgaac tcaacagcta aaatgaatta cggctgtgca ttggatggaa
    31801 tttcaaatac ccaaacaatc aatccttcag gtcttggaaa agaacaagat acaagaatcg
    31861 aaaacttgcg taatgtctta gatggatact tctcaaaagg agcttatcat ttgaatgtca
    31921 atgtattcag taatgattta ttgcgtgatg caatggaaaa tccaatgaaa tatccgaact
    31981 tgacgatccg ggtatctggt tatgcagtta aattccgtga cttgacaaga gaacaacaat
    32041 tagatgtctt gatgagaact tcacacgaca gaatgtaaaa agttcgtgaa aaaagttttt
    32101 cgacttagcg tcgaaaggac gttgaatagc catcgcatca aaatcgaata atcagtatgc
    32161 ttatttgaaa gaaacgattt acaggggcag gacttgtatg ttccgtccct gtaaatttta
    32221 cacataacga agcggcaatt gcgaaaaatt ttgttcagaa agcttcgctt cgacagctaa
    32281 cttgtactta aaatgaaatc aaagaaaaga tgtgaactta aatggaagaa aaaacaatcg
    32341 gctatgttca ttctatcgaa actttcggtt cagtagacgg acctggactg cgctttgtcg
    32401 tgtttatgca aggatgccgc atgcgttgcc aattttgtca caatccagat acttggaata
    32461 ttggcggagg gaaagaatac acagcagatg aacttttaga taaagcagaa cgcttcagac
    32521 cttactgggg agataaaggt ggtattactg ttagtggtgg agaacctcta ttgcagatcg
    32581 attttttgat cgaactattt aaaaaagcaa aagagcggaa gatgcatact acattggata
    32641 catgcggaaa accttttacg tatgaagaac ctttcttttc acgttttcaa gaattgatga
    32701 agtatacaga tttactgtta tttgacatca aacacatcga taatgaagag cataaaaaac
    32761 taacgcattg ggacaatgaa aatattttag aaatggctca atatttgtca aaaatcaaca
    32821 aaccggtttg gattcgccat gtactagttc ctgagcgaag tgattatgat gaatatttga
    32881 tccgactaga caactttatt caaaccttgt caaatgtaga cagagtagag attcttcctt
    32941 atcatacgat gggaaaatac aagtgggaaa cacttggttt gaaatatcca ttagaaggga
    33001 tcgaaccacc tacaaaagaa cgtgtcgaaa atgctaaacg attgcttcat acagaagagt
    33061 atacaaaata tttaacgcga taggtcgcat cggaaagaag ataagtattg cttctttccg
    33121 tttttttgct attattaaat aggagaaatc acaaaggagc gaatgacatg tcaaaaattt
    33181 tagtttttgg tcaccaaaat ccagatacag atgcaatcgg ggcagccatt gcgttttcac
    33241 acttgcaaaa tgaattaggt aaagaaacag aagcagttgc tctaggtaca cctaatgaag
    33301 aaacacaata cgcattagac cactttcagc tatctgcacc acgagtagta gagtcagcta
    33361 aagcagaaaa tgcagatgtc atgcttgtgg atcataatga attccaacaa agtatttcag
    33421 atatcagcga agtaaatatt ttagctgtag tagaccacca tcgtattgca aactttgaaa
    33481 cagctaatcc attgtactat cgtgcagaac cagttggatg tacaagtacg attattttaa
    33541 aactatataa agaaaaccaa gtaacaattc ctaaagaaat cgcaggaatc atgctctcag
    33601 ctatcatttc agatacattg ttgttcaaat caccaacatg tacgcctgaa gatgaagcag
    33661 ctgcaaaaga acttgcagca atcgctgaag tcgatctaaa cacttatgga cttgaaatgt
    33721 tgaaagcagg aacaaatcta agtgataaat cagctgcggt tcttcttgat ttggatgcaa
    33781 aaagtttccc aatgggcagt tccaacgttc gtatcgctca agtcaataca gtggacctta
    33841 atgaagtgat ggatcgacaa gcagagctag aagcagcaat ggaagatgaa atgaaagcaa
    33901 atggctacga tctgttcgta ttagttgtca caaacatctt agacagtgat tcagaattac
    33961 ttgttgctgg taaaggaaaa gaaaaagtag aagaagcttt ccaaacaact ttgaccaaca
    34021 accgtgcttt gcttaaaggt gtcgtttcac gtaaaaaaca agtcgtacct caattgacgg
    34081 ctgtatttag ctaatcaaat ggtagtatga agcattcaaa atgaatggat aagcgattga
    34141 tataaaaaga aacatagaac atactaaagg ccgaagaaat ggaggagaat aacagtcaat
    34201 tatgactctt gttcttcctc ctttttacgt aaaagccttt taagtaaaat aaaacggata
    34261 gaaaagagaa catatatgaa ctattattca aaaaataagg aaaactttta tcaaaaactg
    34321 accgatgatc cgcttttttc ttcattgacc gattatctat atgaacatcg agaacaagaa
    34381 acgattctaa gagaattgaa aaaagaattt tcgcaaaata aattttccca ttttcttgat
    34441 ttattgatcg atgcaggatt aatcaagagg gaagaacgaa gatatcattt gaactttcca
    34501 atatttgatc ctaaagatta cttgcaacaa gccacttcgg ctgctgaaac catagctgaa
    34561 caattgaaga gactctctgt tgatgaacaa aaattagcga tgggagaagt tatttgggcg
    34621 tattgtttcg aagatgaacg gaaagaagcg tatttttatg gagtaagaaa cagccgagag
    34681 acagaattgc tgaggacaac tgcaggaaat gagaagtacc gttttatcac actttcctct
    34741 atagaacact tcccgcttac tttggctaat tacttttttg tacagaagaa tcagcttcct
    34801 gtaacgaagg cattcaaaga attagccgaa cttattggag acgtaaatga atcgtacttt
    34861 tttgatcaaa tagaagttat cgttgacagg atacgtaaga ataaatataa aaatcgccgg
    34921 cctagtattt tccatcagtc tctacttgtg acgaatacta taaaggaaga agaatcattc
    34981 acattagaat tacctattgt cgaaaaaaac aattttgaga ttgaattacc aacattggat
    35041 ccttcattga caatggagga aatggccttt cttaaacgac aaatcttttc tgagctgagc
    35101 aagaaattta taccgcatgc attttcttat atcaaagaat atggaacagt tttagtgagt
    35161 aaaacctaag agagcagtaa gcaaaaatta tactgactct tgcaggagga gcctttctct
    35221 tcctttatgg ttaaaagaat tatttgagta ttttgcctat ctaccaccta ttagattcac
    35281 tacatggtct ggaaacaaag gaatgcaaaa aaatggtgag aaatgtggaa tgacaaagga
    35341 agtagtgata agaaaagtta gatttttaaa taatcaatat tatgattcag tcaagtatgg
    35401 cattttatgg gaagaactag ctgattagat taggaagtcc atttgataat ccagaatcac
    35461 taagaaacga atggcgagtc attaataatt tatccaatct tgggttattc ttttagattg
    35521 aagtgtttaa aactaaaaaa gcatttatga agtcgtaata gtcaggcttc aaaatgcttt
    35581 ttattttgtt tgttttttct ttgccacata aatacaccta gactgacgat gaagagcata
    35641 tccaacagat ttcctgcgac ttggatcgtc gaagggttgg tcagtggcat taaagcaagg
    35701 acaaaaaagg aaaacaagct gtaaataaaa caggtgacag gaactaatcg aaagaagagc
    35761 ggacatttca attggctaaa aatgagtgaa aaaataaaat aagtaaaaat ggataaaatc
    35821 acgactaaac cattcatgat aaagaagaaa agcgtattgt gattaaacga ataaaaaaga
    35881 attgctaaaa aagaaaacgt tttaaatgca atcaatacgc ttgaagcttc gtttaacgta
    35941 ccaggatgtc gatgaaaaaa ataggcaccg aaataaacag aaaacagtaa gccgcaaaga
    36001 gaagacaaaa tcaaaaaaaa cggattttct tcggcttctg tgatatcaga atgatccaaa
    36061 agcaaccagc ctaatgcact agaaggcgca tgtgaggtct taaggtatga ataacataga
    36121 aaaaataaga tcgtcaaagt tgcaaaaaaa gagcctaaat gtaaaaaatt agtggttcta
    36181 cgttgaattt ccataatatc tccaaattaa attgataagt atataaagta taataacaaa
    36241 ttgatgagta tatgtcgatg ttttttaatc aaaataataa ataaaaactg tctaacattt
    36301 aaaatttaat ttagatatta gacagttttt aagtttgtga gttattttcc ttgtcgtttt
    36361 ctcacttttt tagtccagaa accacctgaa atatattttg gctcataaga aatgataaaa
    36421 gcacgctctt cgatttcttt gatcaggcga tacagttcac gttcgctttt tctaggagaa
    36481 agtatttcga gaactagccg ctcaccttcc agccccattg cagaagtctg tgtcacacca
    36541 taaccgtgtt ctctcagtat acccggcaaa cttttatctt ctgcgctatt tgctggcaaa
    36601 atcgttgtga ccatgatgta tcctaatgcc aatttgtctt caatcaaaat tccgacactg
    36661 atacctactg cgtaacctaa cgcatagaca aataaattca gtggattatc taaacgatct
    36721 aaaacaaggg ataagccgac gatataaatc gtgatctcga ccatactgac aagtggagca
    36781 atcagtcgat agcctttcat tgtcaacatg aaacgtaacg tgttcagagt gatataagca
    36841 aaattaatga ggaagatcat ccccaacatt tttggatcaa tcatttgtaa aactcctttc
    36901 atttatataa caacaatatc aaaaaaaatc gtaagtgaag aaaaattgaa actttataca
    36961 tacaaaaaaa tgataaaatg attataatag aattataaag ataggttagg tgattaaaat
    37021 gaaaacggtc aatcgtgtgt tttgtgccag ccacgtgcta agtctccttc tcagtatata
    37081 cttaggtgtc tattcgattc aacagccaat tgaggtagag aacattgttt ttcagatgtc
    37141 gctgacagat ggtttaattt taagcgtttt tttctttttg atactgttta ttggtaatat
    37201 tttcggtagc gttgccagtt ttttgaattt ttctatttat ccactccttt cgctagcatt
    37261 aggagtggtg ggactttgtt ctctgtgctt atttcccgag ccattcagtc ctgcatttat
    37321 attatttggt acactgaacc tgttccagac aactgttgga gcatggttgc tatggcgatc
    37381 aggaaattta atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt
    37441 ccatcatatt ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt
    37501 tgtcatctat tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa
    37561 aattgcgcag cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa
    37621 acagccggtt tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt
    37681 tgaatttgac acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc
    37741 agttggcggg gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca
    37801 tatagtagcg ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat
    37861 cgtaaggcgc gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc
    37921 atgggttaag agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt
    37981 cttttggcat ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga
    38041 aaaaaatcgg cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg
    38101 acatatggta caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat
    38161 aaaaaagtat aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt
    38221 ttattttggg ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga
    38281 caacatgtta gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat
    38341 gtttcaaaaa tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg
    38401 aaagagttag ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa
    38461 gcacttgtca cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca
    38521 actgagccct attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat
    38581 tattctctcc aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc
    38641 atcacaggtg tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc
    38701 ttgtttgggg aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca
    38761 gcaaaagcaa cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc
    38821 gaagtggacg agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa
    38881 gttgatcttc ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat
    38941 atgacagaaa actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg
    39001 ctagcactag gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt
    39061 ggtgtgattg gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg
    39121 atcaagcaag atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata
    39181 gaagaaaaag gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag
    39241 acaacgaatt aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa
    39301 aataatcagc gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca
    39361 tttttgagaa ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg
    39421 tacgatgatg agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga
    39481 caagaagatc ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat
    39541 ttcacgaaca cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag
    39601 tcagctccgc gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc
    39661 cattaccaat cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt
    39721 tctgtcagac aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt
    39781 gacacgaagg tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc
    39841 ccagacccta gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg
    39901 gatgactctt atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt
    39961 gagccgtctg tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa
    40021 gaaaaaaaat cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat
    40081 gatcaaggaa actctttggg agaaaatagc tattttaaat aagtgatata caaatttata
    40141 ttcggctaca gttggaaagg aaacgctgcg gcgtttcctt attttatact ctatccgaaa
    40201 gtttatatcg tatcctcaaa tttttttgag tttaagcgaa ttttttctcc caataagtaa
    40261 gaaaataatg gcaaaataaa aaaacaaact attttcgcag atcgtttgca aaaatagttt
    40321 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
    40381 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
    40441 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
    40501 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
    40561 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
    40621 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
    40681 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
    40741 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
    40801 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
    40861 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
    40921 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
    40981 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
    41041 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
    41101 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
    41161 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
    41221 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
    41281 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
    41341 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
    41401 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
    41461 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
    41521 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
    41581 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
    41641 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
    41701 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
    41761 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
    41821 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
    41881 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
    41941 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
    42001 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
    42061 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
    42121 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
    42181 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
    42241 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
    42301 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
    42361 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
    42421 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
    42481 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
    42541 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
    42601 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
    42661 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
    42721 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
    42781 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
    42841 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
    42901 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
    42961 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
    43021 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
    43081 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
    43141 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
    43201 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
    43261 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
    43321 taaattaagc ggaaaaataa cagaggatca aatgattgat atgaattacc aagtcaatgt
    43381 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
    43441 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
    43501 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
    43561 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
    43621 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
    43681 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
    43741 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
    43801 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
    43861 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
    43921 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
    43981 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
    44041 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
    44101 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
    44161 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
    44221 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
    44281 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
    44341 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
    44401 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
    44461 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
    44521 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
    44581 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
    44641 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
    44701 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
    44761 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
    44821 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
    44881 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
    44941 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
    45001 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
    45061 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
    45121 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
    45181 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
    45241 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
    45301 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
    45361 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
    45421 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
    45481 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
    45541 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
    45601 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
    45661 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
    45721 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
    45781 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
    45841 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
    45901 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
    45961 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
    46021 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
    46081 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
    46141 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
    46201 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
    46261 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
    46321 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
    46381 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
    46441 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
    46501 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
    46561 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
    46621 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
    46681 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
    46741 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
    46801 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
    46861 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
    46921 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
    46981 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
    47041 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
    47101 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
    47161 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
    47221 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
    47281 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
    47341 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
    47401 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
    47461 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
    47521 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
    47581 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
    47641 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
    47701 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
    47761 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
    47821 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
    47881 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
    47941 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
    48001 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
    48061 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
    48121 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
    48181 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
    48241 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
    48301 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
    48361 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
    48421 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
    48481 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
    48541 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
    48601 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
    48661 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
    48721 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
    48781 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
    48841 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
    48901 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
    48961 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
    49021 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
    49081 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
    49141 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
    49201 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aat
//
LOCUS       DACEDO010000012        48363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEDO010000012 DACEDO010000000
VERSION     DACEDO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.012"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(34..2106)
                     /locus_tag="IWO52_000756"
     CDS             complement(34..2106)
                     /locus_tag="IWO52_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0355044.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2039..2416)
                     /locus_tag="IWO52_000757"
     CDS             complement(2039..2416)
                     /locus_tag="IWO52_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0355045.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2545..3105)
                     /locus_tag="IWO52_000758"
     CDS             complement(2545..3105)
                     /locus_tag="IWO52_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0355046.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3255..4547
                     /locus_tag="IWO52_000759"
     CDS             3255..4547
                     /locus_tag="IWO52_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0355047.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4607..5725)
                     /locus_tag="IWO52_000760"
     CDS             complement(4607..5725)
                     /locus_tag="IWO52_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0355048.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5739..7160)
                     /gene="arcD"
                     /locus_tag="IWO52_000761"
     CDS             complement(5739..7160)
                     /gene="arcD"
                     /locus_tag="IWO52_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0355049.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7406..8527)
                     /gene="mnmA"
                     /locus_tag="IWO52_000762"
     CDS             complement(7406..8527)
                     /gene="mnmA"
                     /locus_tag="IWO52_000762"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0355050.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8931..9530
                     /locus_tag="IWO52_000763"
     CDS             8931..9530
                     /locus_tag="IWO52_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0355051.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9668..10954)
                     /gene="efmA"
                     /locus_tag="IWO52_000764"
     CDS             complement(9668..10954)
                     /gene="efmA"
                     /locus_tag="IWO52_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0355052.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11225..11569)
                     /locus_tag="IWO52_000765"
     CDS             complement(11225..11569)
                     /locus_tag="IWO52_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0355053.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11706..12842)
                     /locus_tag="IWO52_000766"
     CDS             complement(11706..12842)
                     /locus_tag="IWO52_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0355054.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13031..14005)
                     /locus_tag="IWO52_000767"
     CDS             complement(13031..14005)
                     /locus_tag="IWO52_000767"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0355055.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14085..14960)
                     /locus_tag="IWO52_000768"
     CDS             complement(14085..14960)
                     /locus_tag="IWO52_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0355056.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15090..16997
                     /locus_tag="IWO52_000769"
     CDS             15090..16997
                     /locus_tag="IWO52_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0355057.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17538..20816)
                     /locus_tag="IWO52_000770"
     CDS             complement(17538..20816)
                     /locus_tag="IWO52_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0355058.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21087..21632)
                     /locus_tag="IWO52_000771"
     CDS             complement(21087..21632)
                     /locus_tag="IWO52_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0355059.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21644..22336)
                     /locus_tag="IWO52_000772"
     CDS             complement(21644..22336)
                     /locus_tag="IWO52_000772"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0355060.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22355..22639)
                     /locus_tag="IWO52_000773"
     CDS             complement(22355..22639)
                     /locus_tag="IWO52_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355061.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22652..23215)
                     /locus_tag="IWO52_000774"
     CDS             complement(22652..23215)
                     /locus_tag="IWO52_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0355062.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23443..24105
                     /locus_tag="IWO52_000775"
     CDS             23443..24105
                     /locus_tag="IWO52_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0355063.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24229..25425
                     /locus_tag="IWO52_000776"
     CDS             24229..25425
                     /locus_tag="IWO52_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0355064.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25526..26902)
                     /locus_tag="IWO52_000777"
     CDS             complement(25526..26902)
                     /locus_tag="IWO52_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0355065.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26939..27805)
                     /locus_tag="IWO52_000778"
     CDS             complement(26939..27805)
                     /locus_tag="IWO52_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0355066.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27894..28919)
                     /locus_tag="IWO52_000779"
     CDS             complement(27894..28919)
                     /locus_tag="IWO52_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0355067.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28937..29611)
                     /locus_tag="IWO52_000780"
     CDS             complement(28937..29611)
                     /locus_tag="IWO52_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0355068.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29795..30688)
                     /locus_tag="IWO52_000781"
     CDS             complement(29795..30688)
                     /locus_tag="IWO52_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0355069.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30762..32459)
                     /locus_tag="IWO52_000782"
     CDS             complement(30762..32459)
                     /locus_tag="IWO52_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0355070.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32452..33765)
                     /locus_tag="IWO52_000783"
     CDS             complement(32452..33765)
                     /locus_tag="IWO52_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0355071.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33765..37283)
                     /locus_tag="IWO52_000784"
     CDS             complement(33765..37283)
                     /locus_tag="IWO52_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0355072.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37576..38034)
                     /locus_tag="IWO52_000785"
     CDS             complement(37576..38034)
                     /locus_tag="IWO52_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0355073.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38331..38849)
                     /locus_tag="IWO52_000786"
     CDS             complement(38331..38849)
                     /locus_tag="IWO52_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0355074.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(38851..39468)
                     /locus_tag="IWO52_000787"
     CDS             complement(38851..39468)
                     /locus_tag="IWO52_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0355075.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39465..40319)
                     /locus_tag="IWO52_000788"
     CDS             complement(39465..40319)
                     /locus_tag="IWO52_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0355076.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40316..41227)
                     /locus_tag="IWO52_000789"
     CDS             complement(40316..41227)
                     /locus_tag="IWO52_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0355077.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41268..42602)
                     /locus_tag="IWO52_000790"
     CDS             complement(41268..42602)
                     /locus_tag="IWO52_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0355078.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            42810..43640
                     /locus_tag="IWO52_000791"
     CDS             42810..43640
                     /locus_tag="IWO52_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0355079.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(43884..44133)
                     /locus_tag="IWO52_000792"
                     /pseudo
     CDS             complement(43884..44133)
                     /locus_tag="IWO52_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44324..44518)
                     /locus_tag="IWO52_000793"
     CDS             complement(44324..44518)
                     /locus_tag="IWO52_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355080.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(45023..45208)
                     /locus_tag="IWO52_000794"
     CDS             complement(45023..45208)
                     /locus_tag="IWO52_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355081.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(46058..46399)
                     /locus_tag="IWO52_000795"
     CDS             complement(46058..46399)
                     /locus_tag="IWO52_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0355082.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(46399..46629)
                     /locus_tag="IWO52_000796"
     CDS             complement(46399..46629)
                     /locus_tag="IWO52_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0355083.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            46916..47254
                     /locus_tag="IWO52_000797"
     CDS             46916..47254
                     /locus_tag="IWO52_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355084.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(47459..47698)
                     /locus_tag="IWO52_000798"
     CDS             complement(47459..47698)
                     /locus_tag="IWO52_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0355085.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(47674..48021)
                     /locus_tag="IWO52_000799"
     CDS             complement(47674..48021)
                     /locus_tag="IWO52_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0355086.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT        14076 a        10307 c         8460 g        15520 t
ORIGIN      
        1 gaccacttct atcacaacct ctttcatatc ttattacttc ttcattgctg cttttagtgc
       61 tgctgctaat gggctttctt ctggttcttc tgcttgtgca tattttttca gcaagcgtcg
      121 ttcttcatgt ttggtcattt tcttcttctg ctctttttta tcaagcaatt tttctgtcgt
      181 tccatcgtat ttacaacgga aataagcacc gtttttacct tcgatgattt ccattttccg
      241 gtgacattgc gggcatcgat gattagagac ttttggatct tttcttctgc ggtagttgca
      301 atctgaattg gtgcagacat agattttgcc gtcacgtgtg tttttctcgc gcagattggc
      361 cccacattct gggcatttct tttgggtaat cgagaaatct tgatattctt gtttgcttga
      421 tttgatctca ttcaccaagc gttttgtttc tttttcgatt ccattcaaga aggttcctgc
      481 ttgtttcttt cctgcagcaa tagcttcaag gtctttttcc cagctttctg tcaaatctgg
      541 tgttactaaa gagggattta ccaatactaa taattgctta cctttaggag atacttgcag
      601 tccttgaggg gtacgttcca tcagttcaga tttgattagt ttctcgataa tctctgcacg
      661 cgtagctggg gttcccagac tgtatttttc catttgacca agcaagcttg cttctgacaa
      721 tggtttaggc ggtgcagata gttctttttt gatggcgaaa tcgggattga ttctcattcc
      781 tttttcccat ttgactgttt ccgtctcagt ttcagtttcg cctttccatc ctgcttggac
      841 gacacgattt tgccgaaaaa tgaatcgttc tttaccaaat tcaaccgtaa cttttgtttg
      901 gctgacttta tggggttgag caaacagtcc taaaaaacga gagacaatca tttgatagat
      961 ttttcgctct tcattactta atttttcata acgtgctctg ttttcagttg gcaaaagtgc
     1021 atggtgatcg gtcactttac tgtcttggaa tacttttgtc tgttggacga tggctccgtt
     1081 tttcaaatac tgcttgacct caggagcaaa gtcgctgact gcttgcaatc gttctttcat
     1141 cgtacttttc atgtcatttg tcagatattt gctgtctgta cgcggataag taacgatctt
     1201 atgggtctca tacaaacttt gtacaagact taatgttttc tttgcagaat agccatagcg
     1261 ttgattggct tctctttgga tctctgttaa atcataaggc aatggcgcag attccgtttt
     1321 taatttttct tggacttctg tgacagttcc tttgcttgta ctgatcaatt gaacaagttt
     1381 ttgtgcttct tcttgtgttt ttaaagcaaa ttgatttttt tgaagcattt tggcttgttc
     1441 ttttccagct tgcaacgtga tcgtgaaata cgtttgtgga cggaactgct cgattttctt
     1501 ttcttggtct ctcactaatg ctagtgtcgg tgtctgcaca cggcctgctg ataaattatc
     1561 cgcgtacttc acagttaagg ctctggtcac attcaatcct accagccagt ccgcttttgc
     1621 ccgggcaaga gcagattcat acaatgcatc atattctttc gcaggtctta gttgcttgaa
     1681 tccagttttg attgctttat ctgtttgaga agagatccat aaccgcttca ctggtttatt
     1741 gaaatgcaca tactctaaga tccagcgagc aacaagttcc ccttctcttc cggcatctgt
     1801 cgcaatgact gcttctgaga catcttttcg ctgagccagc tgcttgattg cttttaattg
     1861 atgtcctgtt ttaggaagtg gtttgattcc tagtttttta gggatcattg gcaacgtgtc
     1921 catttgccag ctctgccatt ccttattcag atcttccggc attttcaatc ctaataaatg
     1981 accaagggcc caagtaacga tcaccttggg tccttcataa taatttttgt gcttttgatt
     2041 agcaccaagc accttgctta ggtctttcgc aacgcttggt ttttccgcta gaattagttg
     2101 tttcataaaa ttcctttctg ctttctactg tgatgggctg atgatttttc aacaacgcaa
     2161 gtcccttgta ttgctgtaaa tcatcgtcac actcttcaca ccagcgctca tctccgtcat
     2221 tccaaaaggc gctgagaaag ttactttttg cattgatata ttcataattt tgatggattt
     2281 cgttccattg tttttcgtac gcttgtcgtg cttcttcgaa agactcgaat gtttcttctt
     2341 cttcaatatc ttcttcccag tcttcaaaaa accaccaagg ctcattgtcc ccgaacatgg
     2401 taattacttg atacatactc gtgctccttc tttcagcttt tctagactca tcttagtcac
     2461 ttttcaagaa agttgcaaga attttctctt ataaaagggt catttaatga acatcttaaa
     2521 attgttacaa atgaatctga ttgtttaagg caaagtagcg ataaagttat tgatcatcca
     2581 aaaatagctg tcttctggat cgattgttgg ttcttttgta aagtatccta aaaaacgtaa
     2641 ggaaagataa ccgtggatca tactacgaag ttccctgctt cgatgaactt tttcttcttt
     2701 cgttaattga aaagcctcaa atatctgcaa aatgatctga ttcgtttcat ggattccatc
     2761 taataaaaca gcttcattgg tatgcggtac actgatcagt aactcataca ctgcttgatt
     2821 tgaaaaagca aaggatcgat aagtttctgc atagatctgc agtgcttcct gaccactttt
     2881 tcccacaaga gcccgtcgca gctggtcatt aagttcctgc agaagtttcg ctgttagcgc
     2941 tgttttgaca tcacgaatat ttttgaaatg gttatacagc gagggagcct tgatatccaa
     3001 gcgtttggct aaatgctgaa aggttatttg ctgaacatcc atctcttctg ctagttctcg
     3061 aaagcagtcg atgattttct cttgagacag atttcttttt ttcatttcca tgtttcctct
     3121 cataaaataa atccttcact ctttcagttt accacgaaaa agaaagagaa aaaagagact
     3181 ttcattgata aactacatat tgtagtttat aataaagagc tgtagatgaa aattttcaga
     3241 aagaaggagt ctaattggct gcaagaataa aggaaaaatt atttaggaaa agagaaactt
     3301 gggaacaaaa cttgtttgtc ttatggtttg gaacatttat ggctggtatc ggatttagtt
     3361 tagtcatgcc ttttatgtca ctttacatag atacgttagg taattatagt acgtcacaat
     3421 tgaacttttg gagcggattg actttttcat ctactttctt ggtgactacg cttatctctc
     3481 cttggtgggg aaaattagcc gaccaaaaag gaagaaaatt gatgctgttg cgtgcttcat
     3541 taggaatggc aatcgtcatt tctttgatgg gttgcgtgac tagtgtgtat caattagtcg
     3601 cattgcgtct gcttcaaggg attttttctg gttatatcag taatgccact gcccttgtcg
     3661 caactgggac gccaaaagaa aaaagtggtc aagttcttgg aaccttagca actggttctg
     3721 ttacaggaac attacttgga ccgcttcttg gcgggatctc tgcttcagcg tttggctatc
     3781 gccctacctt tttcattaca gggatcatct tatttttcgt cttcttgttg agtctgtttt
     3841 tcgtccatga aaaatttgtc ccagtgaaaa aagaaaacat ggtttctgcc aaacagattt
     3901 tcaaagactt gaaatatcca cacgtcgtca tcggtatgtt cgtcacaacg atgatcatcc
     3961 aagcatccaa caattccatt agtccaatca tcagtctgta cattcgccaa ttgctgcatg
     4021 gacatgggaa tgttacgttg atcagcggaa tcattgcttc gatacctggg attgccactt
     4081 tgatagccgc tccaagattt ggtcgcttag gtgacaagat tgggagtgaa agaatacttg
     4141 cgattggttt gggttttgcg attctggttt atatcccaat ggcattcgtc acgaatgttt
     4201 ggcaattagc tgccttacgt tttttgattg gtatctctga tgcttgtctg ctccctgctg
     4261 tccaagcatt gatcacaaaa tattcgcctc atcaagctgc tggacggatc ttcagttata
     4321 accaatcttt ccaagcaact ggtaacgtag tagggcctat gatcggctcc agtgtttcca
     4381 gtgcatttgg ttatcgcggc gtctttcttt cgacttctct tcttgttttg gtcaacttct
     4441 tgcttgtacg acacaataca caagagatcc atcagcaaga aactgcagaa gaaaaagctt
     4501 caggagtctc catagcttca gggcactctc cttctcatag acaataagcg taaatgaaaa
     4561 acactgcttg aacagttgtc acagactgtc cttgcagtgt ttttgtttag gcctgttttt
     4621 tcaagaactc agataagagg aataatccct tttccaaggt ttctttttta caacagtaac
     4681 ctagtcgggc atgcttcggt aaatcaaagg cacttcctgg tactaatagt actcctgttt
     4741 cttctaataa attgatacaa aatgtctggt catcttcttc gatggctaat ttaatgaaag
     4801 aagtagagac gtaattggga gcgacaagtt caactttcgg ttcattggct acccattgtt
     4861 ttaaaatcac aaggttctct gtaatgatct ttttgtttcg cgccaatatt ttttctttat
     4921 tcttcaatgc atgtacagca agatcatctg acaggactcc gccgcaaatc atcgtgtagt
     4981 ctcgatactt tcgaaaaact tctgccaact caggtccagt agctgtccag ccaatacgaa
     5041 tacctggaat ggagtaggtt ttggataggg aattggttgc tatacctttt tcatagacat
     5101 ctacgatcga caagaattca cctttatccg ttaaaggcgc atatacttca tctatcaata
     5161 catatgcatc tactgtacga gcaatttctg cgattttttc taaagttttc cgatctagca
     5221 atgtgcctgt aggattgttt gcactattca aacagatgat ttttgtttca ggtttgacta
     5281 aggcttctag ctgttctaaa tcaaactgcc aatcttcttc ttccttcaaa tgaacaaagt
     5341 caacagcggc acctaaagat ttggggatgt catataattg ctgataagta ggaagcatcg
     5401 aaacaacatg gtctcctgct tccactaacg cgtatagagc taaaagatta gcaccagtcg
     5461 ctccattcgt ttgtaagatg ttttccggat cgacggtttg ataaagagaa gcgacttctt
     5521 gtttgaaaac atctgatcct tcgatccagc catagtccaa tggttcgttt ttgcgttttt
     5581 cgaaaaattt acttacgcta gtcccatcta agccaacgag ttcgtctaat gtcaatgctt
     5641 cgatcgagct ttgtgcaata tcatataccg ctttcttttc ccatttattt agccattctt
     5701 ctacaccgaa atttgcgatt ttcatgtttc tttccccctt atatcttgat ccagcctaca
     5761 gcaagcaacc cgattgaaag cagtgcggcc acacaaatca gcccaatcat tactttttct
     5821 tttcccgtaa tttgatgttt ggtttcacga caagcgatcc agtagaagca aaaaccagga
     5881 agataggcaa tactacataa aaggacataa gaaaatcctg ctaaaatgat gcccatcaat
     5941 tcaaaaacta cagcaatccc accgatcact acttgttttt tgattccttc ttttaaagaa
     6001 aactggactt gatagatccc gacaagtaga taacaaatca aaattgccgc tgtacataaa
     6061 gaataggcaa attgataggc ataatcagta actaagaaag tcaataagaa tagattagtc
     6121 aagattgcag taacaactaa cgaataagtt ggggcatttt ttttattcgt ttttccccaa
     6181 cctttcggca acagaccgtc tcgagccatc aacatcgttg tttctgctgg gagcatcgtc
     6241 caagacaacc aagaaccaat gatcgaaata atcaagccga tattgatcag cgctgctccc
     6301 caatttccca cgattccttt taaaatattt gccatagcag gttgtgaaat agttgctaac
     6361 tcttcttgag ataagacgcc gtatggcaag atcgaagcaa ggatatagat aaacaataaa
     6421 caaagcagac caatgatcga tgcttgctgg gcttctgaac gtttcttcgc gcggttggct
     6481 aaaacacttg cgccttcgat ccctacaaag acccacatca taaccatcaa acaaccttta
     6541 atctgcaacc agatcgttcc ttgctgccct gttccattca ctagattatc agatacgtta
     6601 ccccaaaaat cagcagtgaa tatattcact ttaaatgcga taaatacaaa tattaaaaat
     6661 aggaataatg gcactaattt acagatcgtc acgattgtat tgatgacgct cgctccttca
     6721 actccattgt ttactaataa agtcagaccc caaataaaaa tacttgcaaa taaaatagaa
     6781 ggaacatttt gtccaccttt gaatatcggg aaaaattctc ccatggcgct catcagcatc
     6841 gttgcaaaag caacatttcc cagccacgca gacaaccagt aaccccaacc actgatgaag
     6901 ccacctaatt taccaaatga agcttccgcg taaccgaaaa taccaccatc tagatctgga
     6961 cgcttttctc ctaaatgatt cagcgataat accaaagcta aaataccaat cccaacgatt
     7021 atccatgaaa ggatcgctgg ccctggagct gcagaactag ccagatcact tgtgatccca
     7081 aacacaccgc ttccgattga cgaactgaca accagagcaa ccagtcccca tttgctaata
     7141 cccgtcgatt tcttttccat ttccatcacc ctcacttata aaattgaaca aatcacttgc
     7201 aaaagattcc cttctatttt gcaactgctc ttgcttaaca aaaagcaact ttattcatat
     7261 tattggatat tatgaacttt ttacacaagt atttcaagta taaaatgaat aaaaattaat
     7321 tttattgcaa aaaaacagct atgctatcca aaagaaaagg tgtttctttt gttagccata
     7381 gctgttttga ttcatttaat atcgcttaaa cgtattgtaa aacttttgtt tcttgatatg
     7441 cgtggtcgat caaaccacca cccagacatt ccattccatc ataaaataca actgcttgtc
     7501 caggagtgat tgcacgtaca ggttcgtcga atacgacttc tgcacgatta tcatccaata
     7561 agcgaactgt cacaggaacg tcttgctgac gataacggaa tttcgctgta cacttgaatt
     7621 cttttggcat tggttcattc gtcgtaaagt ggatttcact tgcatccaag cttgtggcat
     7681 acaatgcagg atgatggaac ccttgcccca catataatgt gtttgtcgct agatcttttc
     7741 ctacaacaaa ccagggttct tgcgaatctc ctccgccacc gatccctagt ccttgacgtt
     7801 gaccaatcgt ataatacatc aagccagcat gttgtccttt gacttcacca tcaagagtga
     7861 ccatatttcc tttttttgct ggcagatagt tgctcaaaaa ttgtttgaag ttcttttcac
     7921 caataaagca gattcccgtt gaatcttttt tcttagcagt cgcaaggcct gcgcgttcag
     7981 caattgcccg tacttctgat ttttccatgc cgcctagagg gaacatcgtt tttgaaagct
     8041 gttcttgtga taattggcta aggaagtaag tttgatcttt attattgtct actccacgaa
     8101 gcatgtggac cacgccgttt tcatcgcgag tgacttgcgc ataatgtcca gtagctacat
     8161 aatcggcgcc aagatccatt gcataatcca agaatgcttt gaatttgatt tctttgttgc
     8221 acatcacgtc tgggttaggt gtacgtcctg cgcggtattc ggctaggaaa tattcaaata
     8281 cacgatccca atattctttt tcaaaattca cagagtaata aggaatcccg atttgagcag
     8341 ctactttcgc tacatcctta taatcttccg tggccgtgca tacaccgttt tcgtccgtgt
     8401 catcccaatt tttcatgaat attccgatca cgtcgtatcc ttgttccttt aacaaaagtg
     8461 ctgttacaga tgagtcgaca ccgccgctca tccctacgac aacgcgtgtt ttgctgttgt
     8521 ctgtcatgat atcaccatca tttcaattag attctgaaac atctacgagt tgaaggtcgc
     8581 aacttttcag tattttacat tatacgtcat atttctgaaa atttcatccc ttaattatca
     8641 agtcgtaaaa atactgttct actggaccta ttcagtacac aaacatgata gtcaaccaaa
     8701 aaatcactca tcttttgagc attcgatata aaaaattggc atactcataa aaagggccag
     8761 cctttcggca ataaggaata attttataaa ttgtgaaaaa ctatttttca aaattgcttc
     8821 ttatcgctta aagctgcacg tctcatttac aaccttttct ttttagatgt tatgagttgc
     8881 tcagataagt gattttgttt actctaagaa aaaagggagg caaaagatac atgaattact
     8941 ttatcagtga tatgcacttt ttccatgaac gcttattagg aaaaaacgac tttgctccgc
     9001 ggctctttgc aaatgtggat gaaatgaacc aacagttgat cactagctgg aatgcagtaa
     9061 taaaagaaac cgaccatgtt tatcatctag gcgaccttgc tatgcatccg caatatgaaa
     9121 aagcaaatgc agaaattttg gaactggtca ctcaactgca aggaacaatc cacttgatca
     9181 aagggaacca tgacagtcgc gcttttttcc gttatttaga aaaaaatgat cctaggctta
     9241 ctgacggaag tcccaaattt tatttttatg atgtaggtgc catcgttaaa ttcgaccatc
     9301 aacaatatta cttgactcat tatccgatgt tgctcgggcc taacgacact atccgcaatc
     9361 ttcatggaca tatccatcat tacagcgtac ctatcgagaa cgatgtcaat gtaggcgtag
     9421 acgcaccgga aagagaactc ttaaaggaaa aactaccatt tggcactccg ttatctgagc
     9481 aacaaatcaa agaaatctat gaagcaaaga ggaagtacga taggtcgtga aaaaactgcc
     9541 ctatatcaag tattaaacaa ataaagataa ttactcatat ttttcatttg agtaggctta
     9601 ttgccacagg aacattagga tcagaaatgg agttgtaaca agacttctgt cacaactcca
     9661 ttccatacta ttcgtctttt tgctcatggt gttcttttac cagttttttc tgtaattctt
     9721 tatcataatt tcgggcagct gacgttaaaa ataaaacgat tccgcataat aaaatcccga
     9781 ttccggaaaa caagaacatt ttttctaccc ctaaacgatc agctaatggt cctgcaaata
     9841 ataatccaat tggacctgcc aggttcatca aagagttgaa gttccctaag acacgaccta
     9901 aaacattcga ttcataactc tgctggatca ttgccatcag cagtgtattg aaatatggcg
     9961 ttgcacaacc tgctcctgca ttcaagataa gaaagtacaa gaacccttga ctatcgtttg
    10021 gaaccaatcc gcttgcaccg atcgtcaggc ccataatgat gtaggctata ataataggtt
    10081 tcattcggtc tttccatgtt cctataaaac tgatcagtgc accgcctaag agcattccga
    10141 ctgcgtagac tacttccact gctcccgcct gtccaacgga accaccaaag taatctagcg
    10201 tcatcaatgg atacagactg attgctggca tgaacaacag catcacaaat gcaccattga
    10261 tcgttatgta ccataaccct ttattatcca tcaattgctg cagcccaaat tttgcatctt
    10321 taaaaatagt aagcggaact tccaatattt caggagaaac ttttgggatc ttcacaaaaa
    10381 tcaataagcc gacaccaaaa actgccccca atacatctaa taaaataagg taattgacag
    10441 gaacgactga aaacaaagcc gcacctagag ctggcgcaat gatgaaattc gctgattgga
    10501 ccattcctaa ctgtccattc gtccttgtta tgtgagaaga tggaacgatt gtcggcataa
    10561 tcgactggat cgttggcatt tggaatgttt gtgcgaccga acgcataaac aaagacacaa
    10621 aaactagcca aatcgggaag gattctgaaa tcgttccgac tactgctaaa ataagcgcga
    10681 aaatcgctac aataatatcc gtgacaatca aaagagcttt tttatcccag cgatccacta
    10741 gcggtcctac aaatgggctt agtaaaacca tgggaatcat tcctagtaaa gtagcaaaac
    10801 tcaaaattgt cgccgaaccg gtttctcttg tcaaatacca gatgatcgca tattggacga
    10861 ccatactagt gattcctgac aaaaactgtc ccgataaaaa gaacaaataa tttctcttcc
    10921 aattcgttaa aacgaccgac tgttcatttt ccataaacat ccctcttttc tttttcattt
    10981 tgtatagcgt ttgcacaaca atcattatac gggattcttt tttcaaaaaa tacctttttc
    11041 cctaaataac aatcgtttca atttaaattt ctttacttag aaacaataaa tcagcaaaat
    11101 attaaaaata acaacatgtg ttaaatttaa aaataaaaaa gacaactaat ttgcgtcgtc
    11161 cttacaaagt tagtcatcta acaataggcc gtttcaaaac tagttgtctt tttatatcca
    11221 cagatcattc gactttttcg aataacccac ggctaatcat cccatccatc agaaaataaa
    11281 atttatcttg gctcattttc tgttttggat ctttgaaaat attgactgct ttcaatccat
    11341 ttgctgtagt gatcgacatt ttcaatgttt gaatatcttt tgatacagtg atttttaatt
    11401 tgaatccttc tttgctttgc ggcgtggcat ctaatggtcg gatcaattga taattgcctc
    11461 cgtttgtttc tgtaaatcca ttatcgcgca atgtatatct ttttgcatca ggatgcaaac
    11521 gatagctaat tggtgcgccc agtaccgttg ctgttgtttc aaatgccatg attttcacct
    11581 cgataaaatt agtttctctt tattataaaa gtttatcctg atttttacta ggtttttcag
    11641 ttaagttccc taaaaaatga aggcgttctc tcagacatct gttcatataa gaatgacttc
    11701 actagttatt ttttgacagc acgaactaat tgatcaagga aatactggat gtcgtcttcg
    11761 gtatttccca aaccaaagct gatacggatg gattctttta gtatcggtgt atcttctcca
    11821 tacattgctt ctaatacgtg tgaaggctcg atatttcctg ctgtacaagc tgaaccagta
    11881 gaaatagcga ccccattaag atcaagcttc atcagtagca aatcatttgc ttcgccaatt
    11941 aagcgaagat tcaacacatg agggagttta ttcgttaaat caccatttac ttgataaggg
    12001 atttcgtttg cttctaatgt atcaagaatc aattgctgga atcctttata tttgttgtta
    12061 cgtgcttcac gttcttcagg tgacagcagt tcaactgctt ttgccatacc ccagatccct
    12121 gctaaatttt ctgtacctgc acgccgcttt tcttcctgct ccccgcctaa aagcaatgca
    12181 gggatattta ctccgtctcg tttatataag aaaccgacgc cttttggccc attgatttta
    12241 tgagcagaga tacttaataa atcgattcct aattcttctg gatggatcgt ttggtttccg
    12301 tacgcttgta ccgcatctgt atggaaataa gctggatgat ccgctaaaag ttcaccgatt
    12361 tctttgattg gcatcaaatt tccgatttca ttgttcccgt acatcacaga gaccaagatc
    12421 gtatcttctc gtaacgcttt tttgacctct tcgatcgaca gattcccttt ttcatctaca
    12481 ggcagatatg taacgtcaaa cccctttgtc tctaaatatt tcatggtatt aagaacagca
    12541 ggatgttcga tagctgtagt gattagatgt ctgccttctt tttgacgcga gaacgctgtt
    12601 tctaagatag ctgtattatc cccttctgtt cccccactgt taaagatgat ctcgtgaggt
    12661 tttgcttgca gactgtctgc aatgatttgt cttgctgttt ccaatttttc atgcgcgtgg
    12721 cgaccaaatc catgtatgct tgaaggattt ccaaacgtgt cttgcatgat tgccgtcatc
    12781 gcttcaatta cttgtggatg catgggggta gttgccgcat gatccagata aatagttttc
    12841 acttcattca tcccttccaa attattgcta ggcacattct agcataaaat agcgaacaaa
    12901 ccaaagaaga gctcattttt gtctagagga cgtttcatgg gcatagaaaa aaggagctgt
    12961 gacaaaagtc ttgtcgcagc tccttgaagc tgctcacttc tgtcacaact tctttccctc
    13021 ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc atcggtgtat
    13081 tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg attgttaagt
    13141 tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata tctttgattg
    13201 gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac gcacaggcat
    13261 atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc gttgctcctg
    13321 tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg atcacataac
    13381 caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg ttcaaatatt
    13441 ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact acgatgtcat
    13501 ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta aataaattat
    13561 ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa gtcagtactc
    13621 gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc ggttcatgag
    13681 gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca ttgatcgttt
    13741 tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag taatcattga
    13801 caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt tcttcgatat
    13861 tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat tctgttccga
    13921 acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa atcttcaacg
    13981 tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat tcttcgatag
    14041 tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg aagtgaaact
    14101 gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt gtcgtgtact
    14161 ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac ccaatgattg
    14221 ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc ttcacgtata
    14281 cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc atgattgcca
    14341 ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt aggaagtcct
    14401 agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc cgtatttttc
    14461 ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata ggcaaggaga
    14521 actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt cttatacctc
    14581 aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc tgaccttttt
    14641 tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa ctcgattggc
    14701 tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt atattttgct
    14761 ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg atcttcaaaa
    14821 accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt gctggctaat
    14881 tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg attcagttgg
    14941 gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca gtttgattgt
    15001 tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa gataatgggc
    15061 acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa caagatttct
    15121 atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat aaaccagcta
    15181 caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga gaaacagacg
    15241 atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc cttgcatatt
    15301 atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg cttcaaccta
    15361 taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa tttccagaat
    15421 ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat atgaagaatg
    15481 cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat aagacgtatt
    15541 atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat atggctgttc
    15601 agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg gaacaagcag
    15661 tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac gcagattaca
    15721 gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat aaactggact
    15781 tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc gtaactgatc
    15841 cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg ttattcaaaa
    15901 gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa gaattccgcc
    15961 aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg cctcagaaaa
    16021 aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat tactacggaa
    16081 aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa aaaatgatcg
    16141 ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca aaagaaaaag
    16201 ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag atcccagcat
    16261 tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt tcaaatactc
    16321 ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa ccagtggatc
    16381 gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca tttcggaatg
    16441 tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa caatcaagct
    16501 ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat gcatttgata
    16561 ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca gaagaagatc
    16621 ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt ctgccttttg
    16681 ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat gcaggcggtc
    16741 taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag gaattctttg
    16801 tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa cttttactgc
    16861 aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa aaccttgacg
    16921 aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg ccagaaaaaa
    16981 gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa taagaaggaa
    17041 tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg aagaagttac
    17101 ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag tagtaagaaa
    17161 aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac cgagaaactg
    17221 gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg agtattagag
    17281 aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata ccgagggtca
    17341 gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat caagtaataa
    17401 gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga aggaagtgag
    17461 acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt tttacatcat
    17521 aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac tacaccgatt
    17581 aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt ttttgggagt
    17641 ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga aggttttcca
    17701 tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt gtcatttccc
    17761 gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac agattctgta
    17821 tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc tagcgtatat
    17881 tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc acttgactgt
    17941 gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac aggcgttcct
    18001 tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat gatatttcct
    18061 tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt ttgttcattt
    18121 ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga agacggtgag
    18181 gtttctccgt caaaaatagc acctacagat aatggaagac catccactct ttccttttct
    18241 gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact tccattttca
    18301 tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc gatccaatct
    18361 tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt ccaatagtag
    18421 ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg ctctctcatc
    18481 tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt gtttcctgct
    18541 gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact gctattgacg
    18601 ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa agacaaacta
    18661 ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc attaccgtta
    18721 ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt tttgatgatt
    18781 acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc atctgcatta
    18841 tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt ggccgcaaat
    18901 ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt cttgactaag
    18961 ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg gattcctgta
    19021 tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga aatctgtaat
    19081 cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca gtagtgactg
    19141 aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt gttttctgga
    19201 tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc ttttggaatc
    19261 gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg cccagcatat
    19321 gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt tcctgcatct
    19381 gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc cactgtatga
    19441 agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg atagttttta
    19501 tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt ctgataatca
    19561 aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc tcctgttgct
    19621 ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt atgatcttgg
    19681 tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg agtaggtctt
    19741 tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc ttctaccttg
    19801 atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc tgaagcgtat
    19861 aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc aggatcgtaa
    19921 taatatgcct ctaaaccagt attcgttttt ttcatctcta cgtccagttt agtaatcgag
    19981 ttcgtggccg gaaacagatt cccatgaccg tttccgaata tattataaga acctgtttct
    20041 ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac actcccagga
    20101 gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt ccccaatcca
    20161 agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc cgagtcccag
    20221 acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc ctgcccaaac
    20281 aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg ggcattcgta
    20341 taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc tttggggatt
    20401 gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc gccgttattc
    20461 gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact agcacttgtg
    20521 ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg cttttttgtc
    20581 ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc catatcttca
    20641 ctagttcttg gtatttcctc cattgttgac atcccttcaa tgttcacgtg tgcattttct
    20701 tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa aaccggggta
    20761 ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt taacaagtgt
    20821 attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt tatttttact
    20881 gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat ataacttaag
    20941 ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa aataacgtat
    21001 aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat tttgtgcttt
    21061 ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt ggactcaaaa
    21121 tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc tttgaaaata
    21181 tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca gatatctgta
    21241 catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat gtctagatct
    21301 gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc ctcttgctca
    21361 tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt tcctatcaca
    21421 ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc atgcgcatca
    21481 atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc agtcagcttt
    21541 ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc aacaaaatca
    21601 tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg agatgtttga
    21661 caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat tcgtcaaagc
    21721 tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa aatgggatat
    21781 tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct aatgcttcag
    21841 ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta tcacctgtaa
    21901 cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc atttcactgc
    21961 gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct gggttgtagc
    22021 caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg atgtcaccta
    22081 cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg ttcacgccgt
    22141 attgttggat caacagactt gtggtaatag atgctaagac ttttcctata ccggaacgta
    22201 cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct gcgcgttccc
    22261 atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc attgcgccga
    22321 taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt gctaaaaaaa
    22381 ccacacataa cattaggata acaataataa gagatttcat caaaaaagag ttccatttgg
    22441 tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt ttgctttctt
    22501 tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct gcttttcgct
    22561 gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg cttcgcgtaa
    22621 taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat agttcccagt
    22681 attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc gcttgttgcg
    22741 cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt gctaaaggat
    22801 tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt gcaaaacctg
    22861 gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca gtttcttctt
    22921 ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg atttttccag
    22981 ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca agaagcagac
    23041 gatcttgttc gtcaaaagca atgatcccga caccgccagg atgaaatacc agttctctct
    23101 tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata atttttccgc
    23161 tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc agcatatctt
    23221 ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt tctcaaaaac
    23281 tccttattag tttaccatag ataaacctta gattttctct aaatatagaa aatccttctt
    23341 tagtccgata ctctttatag tagtccgtat gctaaaataa gtatgtaatc gaataacgaa
    23401 aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa acgaaattgg
    23461 ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag tttttctttt
    23521 gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt tggttcgcgc
    23581 aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga aactcactat
    23641 gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat gaatcaaaca
    23701 aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact taaagcaatc
    23761 gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt ggtgaaagaa
    23821 ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa tctagttgat
    23881 ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca gacctatgaa
    23941 aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc acaagattat
    24001 caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt tgcgaaacag
    24061 agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt ttgttcataa
    24121 aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt ctaaagtaga
    24181 gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat ggatcataaa
    24241 ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt attgaacaac
    24301 ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat tgatcagctc
    24361 caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg ccaagaacaa
    24421 gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat cagctacgga
    24481 tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca tgtgcaagca
    24541 caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct ccaagaagcc
    24601 aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt cgggaaggtc
    24661 aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca aattgacaag
    24721 atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct gatgttagaa
    24781 cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat cgctgctgga
    24841 gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa aagagcagaa
    24901 gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt cctagatcgt
    24961 ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca gcaagcacca
    25021 caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca agcatctatc
    25081 gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt gcttcgtcaa
    25141 aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt attgaagaaa
    25201 aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga acgtggaatc
    25261 gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat ccaagagaca
    25321 ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga gcttggacac
    25381 atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac ctttcgcttg
    25441 ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt ttttcttatc
    25501 tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt ccatagccat
    25561 agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc catgtcacag
    25621 tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc aatagcggaa
    25681 cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc aacttcagta
    25741 agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt tccgctaata
    25801 cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca taagtcgctt
    25861 ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt aagccatcct
    25921 tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt accgtcagta
    25981 gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct acatacctca
    26041 ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt cctgtcaaga
    26101 tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg attcctcttg
    26161 gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca tcaaatccca
    26221 agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca aaaggcatga
    26281 atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac aacgcgatga
    26341 taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg gcttcaagcg
    26401 acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg tatgtaccat
    26461 tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa cttttcaaaa
    26521 aaccttgcat atagccagac catccggaag caacagagga aaccgctagt aaaaattccc
    26581 cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca tacagatagg
    26641 catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt agtccagaca
    26701 aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca gcattttgat
    26761 tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca attccaagca
    26821 tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg agattgatct
    26881 ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa aacagcgatt
    26941 aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg caaccaacaa
    27001 taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc ccacctaaag
    27061 tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca agtgatttga
    27121 ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc atgtttttcc
    27181 acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct aagatcattc
    27241 cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg acgatgatgg
    27301 tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta ccaaaatcaa
    27361 gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc aaatacgcac
    27421 cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt tgtgtccatt
    27481 catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca gtagacaatg
    27541 gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt tgtccaacac
    27601 tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt acaggactaa
    27661 caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct agtgtttgtt
    27721 gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat gccaacatag
    27781 ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg ataagaaact
    27841 tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat tttctaacgt
    27901 ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc ttttccttca
    27961 gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc taaataaagt
    28021 cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa tcccttatca
    28081 aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac tgaaaggctg
    28141 actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg cgtatatttg
    28201 atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc ggtcaaaaca
    28261 gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata atttgcctga
    28321 ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag gtagtcccgc
    28381 gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc gatttttgat
    28441 aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat cgaacgaact
    28501 agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc aatataatct
    28561 ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc acggatcagc
    28621 tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact caaataattt
    28681 tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct cttgctcaag
    28741 taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa ataagcaatt
    28801 gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt caatgcaata
    28861 aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa aaaattcatc
    28921 ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt tcgatatatc
    28981 cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact gtcaatgtat
    29041 tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc cgacttaaga
    29101 tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg ctcaaatcaa
    29161 tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg ccattgtggc
    29221 tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat gcattgattt
    29281 ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc cccatgttca
    29341 tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc ggtactttcg
    29401 atacttcacg tattttttga caccaataat agccatccaa aacgggaaga ttaatatcta
    29461 gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg aaatcggtcg
    29521 ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt cgaatgatct
    29581 cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt cttcttgcta
    29641 ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat gtcattttag
    29701 caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt ccaagcagct
    29761 taaatttatt atgttattct taataattgg tctattatct gttatttttc tttcgttgtt
    29821 tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact agcaatatac
    29881 ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct atctttgaag
    29941 agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga gtgttcacgg
    30001 tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt gctaatgtat
    30061 ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc atccgaccat
    30121 acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta aagagttttt
    30181 tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct ccagttggaa
    30241 aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc gtttcatcaa
    30301 ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata tgttgttcgt
    30361 aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga tctttgactt
    30421 gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt tcatgcactg
    30481 ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga taattctcga
    30541 tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat gtaaaaactg
    30601 cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg atttgggttt
    30661 gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa aaaactgcag
    30721 caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc agacttcatt
    30781 ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat tagtaagaat
    30841 gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt cggtactctt
    30901 tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct tccataagag
    30961 atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc tggcgccatg
    31021 gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta tccatttcca
    31081 tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc tgctcctgat
    31141 tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga acggtcttca
    31201 ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc atatttttca
    31261 aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc ataatcaaat
    31321 tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc cgcttctagt
    31381 tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc atctgctaat
    31441 ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt cacgacaaac
    31501 ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt ctcgccggct
    31561 cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc tgtgattttg
    31621 taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata aggaataggt
    31681 gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga aatcgtttct
    31741 tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc tacattcatc
    31801 tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt attgtatgtg
    31861 tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat cactggcatc
    31921 ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt ttctaagaaa
    31981 agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt agagaatacc
    32041 gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata ttttaaaaac
    32101 caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt aagaccgtct
    32161 tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc cgttacgtca
    32221 aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga aaccaatccg
    32281 ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt aatcgtcact
    32341 tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc gaaaatccct
    32401 attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt tttattcatt
    32461 tttttctccc cgttcttttt ttattaataa aacagcacct aacaataaaa gaattcctag
    32521 taaagaaatc aacgttcttg cttcattcgt ttgcgggaat acggaccatt ttcctgattg
    32581 acctggattg cttggaccac tcggctcttt cggtttgtcc gttgtttctt ttacggtctc
    32641 gttataaaca cgtggtgttg cggattcata ggcagagact ggaactttcc cggattctaa
    32701 ttcagccatc tcctgtccgt taactactac atattgtggt tggccttttg catccaccca
    32761 aaattcaggg atgatgacct cgattctctt acttgcttca tccatctcgt aaccttccac
    32821 accttttaat tcttcaaaat aataggtgcc agaaggcaag aaacgttgtc ctgtcgtaac
    32881 aaggccctct ttatccgaaa tgaatttgct tacatttttg tcattcaaag gatcattgtc
    32941 tgctggtttg atccattgat ttttcaagtc attagcagga gccatatcta aataatattt
    33001 ccccccttct atcatctgat atagagcaaa aacagcacct tctaatggtt ttcctttttc
    33061 agttgttcct ctttgacgtc catacttgaa gaagtatgga tctcttagat aaccgacatt
    33121 tttaggatag atatgaatct cttgcagttc ctcggttgat tctgtcgggt gataaattgg
    33181 caaaatagca gcaattggga tagctgtctg atcgatatca acatccaatc caacttctgg
    33241 atcaatcgat tcttcgaaaa tcaaataaac tgcatcccga tcttggtttt tcttaggtgc
    33301 tttgatacgt gcaatcccgt ctcctaatcc gccaaccgta tctttcccag ttgttaccga
    33361 agtccctatt tggacaagcc tttcttctgt gattagctga cggatattgt ttctgttcgt
    33421 ttcagcgatt tgctggacga attctttagg agtcatgttt tcagcagctt ttttttcatg
    33481 ataataatcg gtcatgtcgt aaatggcaaa tgtcgctcca tttagtggca ccgtctgtga
    33541 aatgatatct gctgtttccc ctgcttcctc agagatagcc aagccatcat tttcatggta
    33601 ttcaaattga tcgttataat cgatatctcg aatcatccgt ttatgcaaca cgaattccac
    33661 gacatcttcc cctgattctt ctgccgacat cgcatgtgga agaaggactg ctgcaagcgg
    33721 tagtaaaaca agtaattgaa gcatcaaaca caacagcttt ttcattacct tgctccctcc
    33781 tttttagatt gtctgacaaa caaaccatag gctgctcctg caagcgccat gaagaatgca
    33841 gcaagcagga tatagaataa tcgtccggat cctcctgtag caggcaacgg attttcagga
    33901 tcgttcccga cactaagctg taatctgatt cgatttgtct ccaaaagttc atattctaac
    33961 tcttgctgta gatcaggcgt ctctgcttct tcgtttggat aatacgaaat ctcaactttt
    34021 agtgtacccg cttcttttcc tggaactacc cgatcatttt ctatgtcagg ttcttctcga
    34081 taactaattt ctaaaataaa gtaacctgga agttttctat agctgtcagg tgacttcact
    34141 tccttgatcc gatagacccc ttccaacaag tctgaagcat tgaataggga agaatcgttt
    34201 ggatctggtg ccaagcctcc ctcgatgatt tcatactctc ctgcactatt ttgtttctca
    34261 attgaaaatt ctgcgccttc aagacgtcgg ctattcatac tgtcaatttt caaaagttcc
    34321 aaatcgattg gcgctagttc atcttctaag gttacttgta gtacaccatc gatgatttca
    34381 tagccttccg gcaagggagc agtttcgtct tcgatgaccg tatcttcaat gataaactta
    34441 ccttcttcat tcagtgcaaa ctggaacact tgatcagata gccgataccc atttggtgcg
    34501 acagattccg agacctcata ttcaccaata gacagatgca gaggttggtc gtaatttaaa
    34561 tcgacatact tttctactcc tacggcacga gaatagaagt atccgatccc aggcaaacgt
    34621 ttcgattctt ctggttctaa cggattacca gtgtatgctc gatacagcgg gaaggaagaa
    34681 tcccccactt tcctgatatc gaaacctgca ccatctaatg tatggccttc atggtctaat
    34741 tttctaactc ggatctcccg ataatcaaac gtattgtaag cagtgaatgc tacccataaa
    34801 cgatcctctt catctgttgt atacgtgaga tattgccaat tttcttcacc tgtttgcttg
    34861 agttggatag tccctatagc attatcgtaa agtcgcaaga tccatgtgtc attttcatca
    34921 ggttctttga aaccttctgg gttctggctc tcaatgattt tgtattctgc atagttggta
    34981 gtttcttcac tatccttata aacaaatgaa tctggctctc cgattggatg aataactttc
    35041 tcaccatcca cgattacata tttcccgtct tcatctactg aatagaacgt gagtctccct
    35101 ttctcttcct ctacttcgtt cacaccagaa agccctttcg ctactggata tccgatttcc
    35161 agtccgttac catctgtttc ataaagttca aactctgcgc cattgattgg catattactg
    35221 ccttcaatta gtttattgat attcatgtgg attggcttga attcatttcg catttccagt
    35281 ttgacataca actcatcata ctctgtgcct tctatgttag gctcttgtac aacagaaagc
    35341 ttgtgatatt ctccttcata ataggaattc ggatctttac cttccggtcc gccgactctc
    35401 gaaccagatg tatattcatc tacaagctcg tagtgaccta acatctcatc ttcgttggct
    35461 gcatattttt tgatttggaa ttgatacgtc tcatctacta actgataacg tggtggtcca
    35521 gcgatctctc tgatccaaaa tatgtcgctg actgctccag tttcatctcc taaaatcggc
    35581 atcttcgttt caaagatcgc tattccgtcc gtggtagttt tacttgtttc gattggttcg
    35641 gcttccccac tctctggatt ttggtcatat aatgcaaatt cgactccagc caacggctcg
    35701 ccattttgac ccttcttgct gacttggaaa ttacggcctt tatgttcgta acgaatggaa
    35761 gaagatggga aatacatccg tccagtagtg atatcattct cataatcctc caggttctga
    35821 atataggcca tattgttcac tgtgcaaaaa tttccgtctt gagctcccgc atcaagtttt
    35881 acttggtact tgatcctgac catttcccct tcacctaaag aaatcggaga aaatttataa
    35941 gcttggtttt cccgtccgac atacgttgtc ggagtcgcca ctacttctgg tggggcttgt
    36001 ccatgacgtt cttcatccca tatttctggc tcttggtact tcatgccgtt ttctcctgga
    36061 tctagatggt aaaactgaat atctgccgat ctgccagctc caaatggtga gacattaaaa
    36121 ccagtttcat cataaagtgt ggcttgctgg atcgttctct tgaaggtaga agagatatcc
    36181 tccaaggctg taacaatgtc attcttgttg ttcgcattgt aatatcgcgg tgttacgcca
    36241 tcttcttcta aatcagcaat atttttcaat acatttcgtc caatagcatc tagtcgttgt
    36301 ctcccgctaa cgtttccatc gattcctaaa ccaatggaga agatacttgg cgggttctct
    36361 tgatacttgt ttttcagcca atgatgataa gcaactgtgt tgatcgcaga tgacttttct
    36421 tgcgaagtag gactgccatt cgtattgtta ttagaagaac catcaccaac ccagtgagtc
    36481 gctttattat ctccttcttt tacctctcca taacgatagt gtacatttac ttcatcttct
    36541 ggacggttaa agtcagatgc ataggtaact ggatgagtaa accctgcatt agaagtattc
    36601 ccagaaaact cctcaaaatt tctgaacaaa acgtcattat cttcagcaat cttatcttcc
    36661 atcacagtcc agttgctcca agctccatta gtgctcgtac gataacttga ctgaatcggt
    36721 gcataactga aggtcggtgt cccatctccc acaacgatta ggattttttc tggatttcta
    36781 ttttctccgc cattgtcttt atacaatgtt tcatagccat ttttaagccc tagtgtaagc
    36841 ggagttcctc cgattggtgt acgatttaat gctgtattct cacttcccgt caaacgattg
    36901 atatcatttg tcaatggaaa gatgtccgaa ctgatttgct catggttatt gatagctgta
    36961 ctattatggt aaaaattcac cataccgatt cgaatatcca gactcgggtc agagagcaga
    37021 tctttactca tttcgtttac tgcctcaatc aacgcagcat ttttagtttg actattggtc
    37081 ccagcggtta attcactcat actcgctgac ttatctagaa caaaaacgat atcgattgga
    37141 ctgatttcct ttttctcgcc cccgatgaca tctagataga cattgtaaag ggttgttgca
    37201 tcttcaggat gtagatcaga tgctgtgctg cttggcagaa caggttctac ccattttttg
    37261 atgtacgccc cgttataatc caagtaactg ttttcaaaat tccgttcatt tccccaaagc
    37321 tgaacggttt gagctggaac gtttctatct tcttcgcctg cagttgatcc attattagta
    37381 gcatagtcgt aatttcgcca gttcgctccc ccgtttgtat aatcactatc atcgatcaag
    37441 ttctctccgt catatggcga gcctttgtct ccttgatagc gttcattgct ttgtgtccaa
    37501 tttcctaaca cttttgtata gtcattcgtt gtaaacttca tctattcttt catcaaccac
    37561 cacttgaaga ggtagttacc cttcgttcac atcggggatc ttttctgttt caaaaatcaa
    37621 tgtgccggtt tctgcttctt ttgaataaac ttttccgaca taccattctt gaccatcgat
    37681 cgtctcgact tttaggtctt ctccatcttc atcataggag gatagacctg ttccacgaac
    37741 gttttttacg gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta ttctaccttc
    37801 attggcactt tccttttttt caaaagccag atgccattca agggtttctt ctttttcaat
    37861 atacttccct tcaatttttg catagtcctc atctaagaat acctcatcct cctttgtttc
    37921 tgttgactca gcagctacat ccagggtcgt catatgatag actgactgga aatatggtcc
    37981 aaatagcaaa ctgacagtta ctaactgcaa aagcttcaat tttaaccctt tcacatcctt
    38041 atactccttt caaaaatatt tgtcttcttt cacaacaatc aacaaaacaa tgcccatcta
    38101 attataattt aatttaattt taattttaaa tcaaatgata tcctttttag ttaaaaacac
    38161 caattataca acatttatta ttaaaattaa attataaaat tttataacgt tctcatttaa
    38221 caaaacttaa gttattaaga caaatgaata aacaactacg ctttcctcga aacaaaaaaa
    38281 taaagcacaa atgcgcattt acgcactcat gctttattcg tacaattaat tcagataacg
    38341 gcagcgcata ggatgccaat aacacttcct aaaatagctc ctcctaaaat gtcagtagga
    38401 taatgtaaaa gtaaaaaaat cctcgaataa gcgatcaaac aagcaaataa aaaggctaat
    38461 attcctgcaa ctggacttaa ttcgaacaac gcagctactg ataaaaaggc tagcgctgta
    38521 tgtcctgaag gaaaagaaga agtcttcggt ttacttgtgt aaaggacaag ccgacgattt
    38581 ttttcataag gtcttttccg tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa
    38641 tagatcaaac tgccgataag aatggctaga ccatattctc tagtatcagg tatcaagact
    38701 aaaacagcag caaggagcaa ccaaataaaa ccaccattag agatattact gataaatttc
    38761 aaaaaattga tttttcgact atccgttttc cgttgcttgg cgaacttgtt gataatacta
    38821 tcatcgattt tagcaatcaa acgcaacatg ttattcctcc tttttaagaa ccttttcttg
    38881 cgtacgatgg ataaaaagca ggacttgaaa ataaaacaag aaactgaaac ctaataaagc
    38941 aaaatagatt ggtcctgcaa accagaatgc gacaagaagg atcaacgtgc ctgccaaata
    39001 gaaaagcgcg cttttgaaat cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc
    39061 tttttgttcc agttgaagca agattttgta actaaagaga agcgcgctta atagaagcaa
    39121 aaacaacgta gtatacatca atgtatagcc aattccccca ccccaatagc ggaaaatctg
    39181 ataatcgatc aatagaagaa gaaaataaac acttgctaaa ctatcttgct ttccataaag
    39241 agagaagttc tcacgaaact ttttccagaa tagtgaaccg agctttttct cttcattttt
    39301 tttgtaatga taaatcatat acagaactgt tcgacttgca gttcctaatg tgaaaacagg
    39361 caagacgcac aggaaccata gtagctgtaa atggataaga cgtggaatcc attcaatgat
    39421 ttctaagaaa taatcattgg cttttttacc aatcgatcga ttcatcatcc ttttgaacct
    39481 cctgtcaaat ccatcccttc aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc
    39541 gggatcaaga caagtgttgc tcctgccatc agataattcc attgtgtcgt tgcttggttc
    39601 tgaaaaactt gcaagccaat ctgtaacgtg tatttttctt gatcttggat atataacaga
    39661 ggacctaaga aatcattcca agctgcgttg aaggaattga tcgcaatggt aatcaaggca
    39721 ggtttcgtca atggaagcat caaatgactc caaatataca aatgattcgc tccgtcgatt
    39781 tcagcagctt cgatcaattc attattgatc gataagtaaa attgacgcat cagaaatata
    39841 ttgaacgcat tgccgaagaa cgatggaacg atcaggggca aataagtacc tacccagccg
    39901 atcttggaga ataatacata ttgagggatc atcgttacaa accccgggat catcatcgta
    39961 gaaagaacca aagcaaacat cagctttttc ccaggaaaat caagcttcgc aaatccataa
    40021 gcaataaaag aatttgaaag gacattaccg atcactacta gcacagtgat gaataatgta
    40081 tttcttgcat agcgccaaaa aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg
    40141 attttttctg ggaaaaaggt cggaggataa cgaacgattt cttccattgg tttcaatgaa
    40201 gtaaacacca tccaaagcaa tggaatgatc aaaatgattc ctattactgt catgaggaca
    40261 taacttagta ttttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt
    40321 tgccgtcacc tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca
    40381 gaaccaacac gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag
    40441 ctacgaacgc cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc
    40501 ctgttccatt tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag
    40561 aggtgatcac atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact
    40621 ttccccaaac gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt
    40681 gtaatgtagc taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca
    40741 acgcaggttt cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc
    40801 ccagaaatgt gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga
    40861 ctgctactcc tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt
    40921 tgattttttg attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat
    40981 tgaagatgac gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga
    41041 acattctttt gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg
    41101 tgaaactacc aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa
    41161 taaaaggcga gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac
    41221 gcttcaaatt ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc
    41281 ttgactaagt tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt
    41341 cctaaaagaa tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa
    41401 ggaactggtg tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt
    41461 cctttatccg ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg
    41521 atatcaaaac ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg
    41581 aattcataag aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca
    41641 ccccacgacc aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca
    41701 aaatcatccg gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg
    41761 gctactaatc cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta
    41821 ttgatcgtat ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact
    41881 ttatttttag tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct
    41941 gcgtttagcg cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta
    42001 aattcttttc cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt
    42061 gtcggtagat cttgttcact gattccagct tctttaaata actttttatt ataaaaaatg
    42121 acttgtgtat ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc
    42181 caaagctgcg gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt
    42241 gcttgctgtg cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt
    42301 gcatttccag ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac
    42361 ttataattta catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata
    42421 gcttcttttc gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca
    42481 cttgtttttt cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg
    42541 catcctccta ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc
    42601 atattttacc tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt
    42661 gttctgaaaa taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac
    42721 tttatataat tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat
    42781 gtattaaaaa tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg
    42841 aagcaaaacc agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg
    42901 gccaatatgg cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag
    42961 gagaagaaaa atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg
    43021 aaatgttggc gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag
    43081 cagcgatgca aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg
    43141 cgatcgtagc tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg
    43201 cctatatcat tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat
    43261 cacaaggaag acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag
    43321 atatgctgtc tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag
    43381 tgaaagaatt agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca
    43441 aagaacgaaa agacattgct tatgacttct ataacttctc agagggagac caaagtagtg
    43501 aaggttcttg ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag
    43561 aacctaagat cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga
    43621 cacctccatt caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc
    43681 tttgtgagca aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc
    43741 tctagagcta taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta
    43801 tgttcaagcc atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact
    43861 ccttagccct taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca
    43921 tgtttttctt gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc
    43981 acatagtatc cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa
    44041 tctcttgatg atctcctgaa tattggctcg atattgatta taaattattt ttcttctata
    44101 ctttggtgtg aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc
    44161 ctttattgct ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat
    44221 taggtctaaa gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt
    44281 cagaaaaaaa tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt
    44341 tataaatgga aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta
    44401 tgaaatcttt atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat
    44461 ttatcagaag tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag
    44521 actctccttt cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa
    44581 aggggcgcag aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt
    44641 ttaataacta taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc
    44701 tccaattttt aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact
    44761 tatatatcga acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac
    44821 ttgcatctaa ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt
    44881 aatttttaaa ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc
    44941 tgtctcatcc agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg
    45001 aatagttgag ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata
    45061 caatgaaaaa caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact
    45121 cattactgta tgattttagc agcccaacca acatataccc tataaaagct aataagatgg
    45181 ttaggaatcc tttagaattt ttactcattc atagacactt ccttaaataa cttttattat
    45241 agaaggtatt gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaacttaca
    45301 ttatactgga atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg
    45361 taatcggtaa agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat
    45421 cataaaaggt acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc
    45481 tgacccaatt gcacattttc gatcattaca agtattggtg tcgttagaac aactaaataa
    45541 aaactagcgg tagatttttc agaatctatg ctaatttctg tatggatgtt ccagtgatgg
    45601 caaatttctc ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt
    45661 aaactataga aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc
    45721 gatatagttt aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac
    45781 tgtcagttgt tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta
    45841 agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca
    45901 cctcaatcat tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatgcc
    45961 atcgaccaca ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt
    46021 tagcatataa aaatattata gagtttactt acaatgctta aaacatatac tgtataatct
    46081 gatcaatttt agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct
    46141 taaaatcaag agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag
    46201 gtaatgagta acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa
    46261 acattgtttt acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa
    46321 tttcttttcc tacggaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat
    46381 aaattctttc ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc
    46441 gtctttctca taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac
    46501 taacgtaatt gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt
    46561 ctttccgtta tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt
    46621 aacaagcatt atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa
    46681 attccccttt gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata
    46741 aatagaaata aattgtagcc agttcatttt ttctttaact aaaaaaataa tccaagaaac
    46801 attgaaccta tggatccaaa agctacccaa atactatagc taaccccccg ctaccttcaa
    46861 ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca acaggccatc ctatcatgcc
    46921 cgattataaa caaaaagctg gctattcctt ttgctctgac aaatttcaat tgggaaagtt
    46981 cacgaaacta gcaggtgaag aagtttcaac tgttcggatc aaagaatgcc cgcttcaaat
    47041 agaaacattg gttcaacgaa tttcaactag acaagacttt gctattgtag aacgtgagat
    47101 cgtagctatt tttgtagaag aagggatttt acatgatcca actcatatca atgtggataa
    47161 atggcaacct cttatctata aattcagaga atatacaact gccaaccagt cgttaacttt
    47221 gaattttaga ttccaagaat tcaaagagtc ataatgataa acatcgttag gtataaataa
    47281 aaaagactcg cagcacgagt aaaaaacaaa tagaagctat acattagaaa ggtcgactgt
    47341 gaccctatag atgcagtata tgcagcttat gagcatttct tctgttctct ttgtttttgg
    47401 gatctgtgct gaagtctttt tcgttttttg ccgctctttc tttgaaaaat cgattatgtt
    47461 ataccttctc cattactaat tcaggcatct gctgagtaat gatttttcgc gcaaaagaaa
    47521 tatctgttac ccaaggttcg atttgttctt cagtcaagac aagcggcatt cggtcatgga
    47581 ttggtgcgac tgtgcggtta ggttcagtag tcattatgat tgattctgcc attttttcat
    47641 cctgatggct acgataaaaa ccgccaatat acactatttt ttccttattt gaaaacataa
    47701 atttgtcttt ttcttcattc cactcattgg gaaaacacaa cgattttgca aaaatggttg
    47761 atgaaaggtc tttttttctt ctactgtctc tgcacgggca ttaatcatca attgtccttt
    47821 tttaaatccg gtaaatcccc acttcatgat ttccggagtg aggatgtttt cttcctcttt
    47881 tcttaacgtc acaacaagat ttgacggaaa aacttctcca gctgctaatt ctgctgcctg
    47941 cttcggttgt ttcgtctggg cttctcgata ataattctga aggagctctg actctagatc
    48001 aaaaaaataa cgaccacaca tcctaatccc tcccaatctt tttttctatc atacactgca
    48061 acaagaagtt cgcaccaaca aaacgctttc aatttttgta gaactaaaaa agttgtgaca
    48121 agtgacaaac tctccagtga atggtattct cctgccaact cctcggtcac aagtcacaat
    48181 attcaacaaa catgagaagc tgctttttga aattgttcct tgtgactcgg agtgaaacgg
    48241 caggcttacg acctctgggt aaacggtgtt caaaagccag tttctcggta ttaagccaaa
    48301 tttatgcaaa aatgtagaaa gctattttca caaaattttt cttaatactc gaaactaagc
    48361 gac
//
LOCUS       DACEDO010000013        45667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEDO010000013 DACEDO010000000
VERSION     DACEDO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.013"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..243
                     /locus_tag="IWO52_000800"
     CDS             4..243
                     /locus_tag="IWO52_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355087.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            310..519
                     /locus_tag="IWO52_000801"
     CDS             310..519
                     /locus_tag="IWO52_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355088.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            579..1250
                     /locus_tag="IWO52_000802"
     CDS             579..1250
                     /locus_tag="IWO52_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0355089.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1303..3279
                     /locus_tag="IWO52_000803"
     CDS             1303..3279
                     /locus_tag="IWO52_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0355090.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3292..4047
                     /locus_tag="IWO52_000804"
     CDS             3292..4047
                     /locus_tag="IWO52_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0355091.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4044..4868
                     /locus_tag="IWO52_000805"
     CDS             4044..4868
                     /locus_tag="IWO52_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355092.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5157..7268
                     /locus_tag="IWO52_000806"
     CDS             5157..7268
                     /locus_tag="IWO52_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0355093.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(7306..9363)
                     /locus_tag="IWO52_000807"
     CDS             complement(7306..9363)
                     /locus_tag="IWO52_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0355094.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9477..9689)
                     /locus_tag="IWO52_000808"
     CDS             complement(9477..9689)
                     /locus_tag="IWO52_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355095.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9876..10343
                     /locus_tag="IWO52_000809"
     CDS             9876..10343
                     /locus_tag="IWO52_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355096.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10340..10582
                     /locus_tag="IWO52_000810"
     CDS             10340..10582
                     /locus_tag="IWO52_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355097.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10600..10902
                     /locus_tag="IWO52_000811"
     CDS             10600..10902
                     /locus_tag="IWO52_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355098.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10974..13028
                     /locus_tag="IWO52_000812"
     CDS             10974..13028
                     /locus_tag="IWO52_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355099.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13028..15640
                     /locus_tag="IWO52_000813"
     CDS             13028..15640
                     /locus_tag="IWO52_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0355100.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15637..18168
                     /locus_tag="IWO52_000814"
     CDS             15637..18168
                     /locus_tag="IWO52_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355101.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18220..18552
                     /locus_tag="IWO52_000815"
     CDS             18220..18552
                     /locus_tag="IWO52_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355102.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18553..19146
                     /locus_tag="IWO52_000816"
     CDS             18553..19146
                     /locus_tag="IWO52_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355103.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19309..21003
                     /locus_tag="IWO52_000817"
     CDS             19309..21003
                     /locus_tag="IWO52_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0355104.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21691..23568
                     /gene="ltrA"
                     /locus_tag="IWO52_000818"
     CDS             21691..23568
                     /gene="ltrA"
                     /locus_tag="IWO52_000818"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0355105.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23699..24076
                     /locus_tag="IWO52_000819"
                     /pseudo
     CDS             23699..24076
                     /locus_tag="IWO52_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24076..25281
                     /locus_tag="IWO52_000820"
     CDS             24076..25281
                     /locus_tag="IWO52_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0355106.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25297..25941
                     /locus_tag="IWO52_000821"
     CDS             25297..25941
                     /locus_tag="IWO52_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355107.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25952..26758
                     /locus_tag="IWO52_000822"
     CDS             25952..26758
                     /locus_tag="IWO52_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355108.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26780..27010
                     /locus_tag="IWO52_000823"
     CDS             26780..27010
                     /locus_tag="IWO52_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355109.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27556..27831
                     /locus_tag="IWO52_000824"
     CDS             27556..27831
                     /locus_tag="IWO52_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355110.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27873..28295
                     /locus_tag="IWO52_000825"
     CDS             27873..28295
                     /locus_tag="IWO52_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355111.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28314..29906
                     /locus_tag="IWO52_000826"
     CDS             28314..29906
                     /locus_tag="IWO52_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355112.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29918..30253
                     /locus_tag="IWO52_000827"
     CDS             29918..30253
                     /locus_tag="IWO52_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355113.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30400..30594
                     /locus_tag="IWO52_000828"
     CDS             30400..30594
                     /locus_tag="IWO52_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355114.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30626..32998
                     /locus_tag="IWO52_000829"
     CDS             30626..32998
                     /locus_tag="IWO52_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0355115.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33059..34519
                     /locus_tag="IWO52_000830"
     CDS             33059..34519
                     /locus_tag="IWO52_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0355116.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34530..36734
                     /gene="topB"
                     /locus_tag="IWO52_000831"
     CDS             34530..36734
                     /gene="topB"
                     /locus_tag="IWO52_000831"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0355117.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36973..37566
                     /locus_tag="IWO52_000832"
     CDS             36973..37566
                     /locus_tag="IWO52_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0355118.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37582..38481
                     /locus_tag="IWO52_000833"
     CDS             37582..38481
                     /locus_tag="IWO52_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0355119.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38484..38969
                     /gene="ssb"
                     /locus_tag="IWO52_000834"
     CDS             38484..38969
                     /gene="ssb"
                     /locus_tag="IWO52_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0355120.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39335..39595
                     /locus_tag="IWO52_000835"
     CDS             39335..39595
                     /locus_tag="IWO52_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355121.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40036..40242
                     /locus_tag="IWO52_000836"
     CDS             40036..40242
                     /locus_tag="IWO52_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0355122.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40242..40493
                     /locus_tag="IWO52_000837"
     CDS             40242..40493
                     /locus_tag="IWO52_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355123.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40508..40945
                     /locus_tag="IWO52_000838"
     CDS             40508..40945
                     /locus_tag="IWO52_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355124.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40938..41645
                     /locus_tag="IWO52_000839"
     CDS             40938..41645
                     /locus_tag="IWO52_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355125.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42026..42205
                     /locus_tag="IWO52_000840"
     CDS             42026..42205
                     /locus_tag="IWO52_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355126.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42546..42809
                     /locus_tag="IWO52_000841"
     CDS             42546..42809
                     /locus_tag="IWO52_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ0355127.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42803..43153
                     /locus_tag="IWO52_000842"
     CDS             42803..43153
                     /locus_tag="IWO52_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0355128.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43177..43791)
                     /locus_tag="IWO52_000843"
     CDS             complement(43177..43791)
                     /locus_tag="IWO52_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0355129.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44105..44527
                     /locus_tag="IWO52_000844"
     CDS             44105..44527
                     /locus_tag="IWO52_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355130.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44537..44740
                     /locus_tag="IWO52_000845"
     CDS             44537..44740
                     /locus_tag="IWO52_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355131.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44951..45535
                     /locus_tag="IWO52_000846"
     CDS             44951..45535
                     /locus_tag="IWO52_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0355132.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16748 a         7210 c         9265 g        12444 t
ORIGIN      
        1 tagatggcgc gaccagtttt agaggcgatg gatatggata ctttgacaaa aggaaggtat
       61 gtacagctgt tagctcgtgg ttattcacct aaagaagtat tcaaagcctt atctaaagga
      121 acagatatgg aaaaagaaag attgcgaaaa gaattcgatt attggagaac ccataatgga
      181 atcaaggatt tgaaaccagc acggccagta attaaacgaa agaaaaagat aacccaagac
      241 tgaagtgtag agcgtatgtg atcataggct tttttatttt tgcaaaaaac aaggaaggta
      301 ggaaaactca tgaattatga agaaatcgaa aacagaaaaa aagtgtcgaa agaaatggaa
      361 gaaaagttac taaaaacgat gaagcaaaaa caccttaaaa gattatctgt cgcgcaatat
      421 atcaatgata tgcagttaac aggaaaagag aaagcttgtt tgttaggttc aatgaaaaac
      481 tttgaacagt tgagacgaac ttatagaatt catttttaaa actttttcta tggaaatgag
      541 aaaaagtttt tttgttttgt ctgaaaggag gcagatggat gaaaaaatgg ttatttggtt
      601 ttttaggagt cactttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac
      661 atgaaggaga ggtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg
      721 cacatgcaaa gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc
      781 aaatgttgtc catacaaaaa gaagcaatcg aaatgggcgt caataaacaa gtatttgcac
      841 aaattcagat tcctgcactt ggtttagcat tgccaatttt taaaggggca aatcaataca
      901 ctttgagtct tggagcggct acttattttt atgaagatgc cgaaatgggc aaaggaaact
      961 atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat attcaaaagt
     1021 tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa
     1081 cgcgtaaatt tatcgtacct gggtacttta aattaatcga tggcgttcct gaagagaact
     1141 ctttcttgtc tttgccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt
     1201 atacgtcaca aggaaaagaa agatacgtgg tacagggaga gctacaataa caaggtttta
     1261 atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggtat
     1321 gtaaaaaaag ctggaatgct tgttggaatg ttaggaatca ttgcaggctg ctttcagggg
     1381 tatcaagtac atgcagaaga tgtcacgcaa aaaacaccac cagagaaggt caatatcacg
     1441 gttcataaat taatgtacga tcaaggaacg caattgaatg ttgatattga cgggatcaaa
     1501 aacgatggtt atacgcacga tcaatatcct acaggtgtaa cgaaatacaa caaggcagat
     1561 tatggtgatg ttgagtttac gttaggaaat attacggatc aagtattacc gagagaagat
     1621 tctgatctaa ccaacgcgaa agtggacgag attgtacaag atgtagaaga caagggatct
     1681 aacagtgagt acgtcaagaa tgcaaccaac aaaattactt cagctgtaga tgaaaatggt
     1741 gagatcactt ttactgatca accagcctat gtcaattcca aagggaatgt atatgtggtt
     1801 tatgaaagta aatccgctgc tggattagtc acacaaaaag caaaaccaat ggttgtgatt
     1861 gcgccaatga cagataaaac aggatcagga tttttaaaaa atattcattt gtatcctaag
     1921 aacattgttt ctaagttgag ttttgaatta acgaaatttg gtgacgatgg cgcagaccaa
     1981 agcaaacaaa cccttttaaa aggggcaaaa tttgagttat acaagggcga acctggaaaa
     2041 ggaacaaaat tgggtgatct tgtatcggat gatcaaggaa agctgactgc gactgatttg
     2101 acgttaggaa aatactattt tgtggaagtt ccttcggaag ttgtagtggg atcagatcaa
     2161 gaaccaacag cagatcaata tttattaggc gcagacgctc gcaacgatgc gcacaataaa
     2221 ttaacctttg aaattacgaa tgatggcgtg acaagtgact taaaagcttc atacgtaaac
     2281 tataaagcac cagtgatcga taaaacagta acaaatggaa caggacaaga acattctttc
     2341 caaattggtg acgcagtaaa ttatcaagga acgattcata ttccaacaga cattgcaggt
     2401 ggcgcagacg gtatcacagt aaacggtgtg aaaagcgaaa cttctcctta cagtgtattt
     2461 aaatggggag atacagcagg taaaggactt tcttatgttg ctgaaaaagc agatattaaa
     2521 gtaacaaata atgatggttc agtcgtatta aaagaaaata ctgactataa aatcgaaaat
     2581 tctgatagtg gttttgtgat cgattttatc gtaaataatg gacaagtaag tgatacagtt
     2641 gcgaatttac atggtcaaga tctaaaaatg acttataaca tgtttgtgaa tgatagtgct
     2701 gaaatcgcaa atccattaac aaatagcgtg gatttcgttt ataacaataa tccatttaat
     2761 caggaagaac accacgaaaa aacgaaagct gacgttgtga cttatggcgc aaaattctta
     2821 aaagtcgata gcggcctatt tggaacaggt atcaaagcaa caccgcttga aggcgcagaa
     2881 tttgcagcta agaatgcaga aggtaagtac tacggtggac ttactgatac agataaagat
     2941 ggtgtcaaag aagcagtttg ggtagacgat gtagcaaatg cagcaatcct aaaatcagat
     3001 aaagagggtc attttgaaat cactggttta actgaaggag attactcact ggaagaaaca
     3061 aaagctcctg aaaactatca aaaattaaca aaagagatct ctttcaaagt agacaaagat
     3121 tcatacaaag aagaaaaccg aatcacgatc aaaaataatc aaaaagcttc cgtaccaatg
     3181 actggtagca acggtttcca atcttacgta ttgatctctt gcttgttgct aggagcaggg
     3241 ggactttctg cagttgttta ctttaagaag aaagcctaga aggatcagct aatgattatt
     3301 cgtaagcccc caaaaagaag agtcatgcac aaactgatga tatttttttg gttgctgtcg
     3361 gcagtagcag tctatttaat tccagttgtg gaaacaaatg tagcgaaatt tcaacaacag
     3421 caaagaattt tagccattga aaatcttgaa aaaacagatt cttctttgga tgttgcgaaa
     3481 atagagttag gcgatcctgt tggtgttctt acaattccaa gtatttcttt aaaactacca
     3541 atttatgacg gaacttccga caaaatttta gagaatggtg tagggattac ggaaggaact
     3601 ggcgatatta caggaggaaa cggtaaaaat cctttaatcg cggggcacag tggactttat
     3661 aaagataatc ttttcgatga tttgccttca gtaaaaaaag ggcaaaaatt ctttattaag
     3721 gtaaatgaag aacaacatgc ctatcaaatt tatcgaattg aagaagttaa agcagaagaa
     3781 ttacaaaaaa attatttaga ttatcttatg cctgaaaaag accaagatag gatcacgtta
     3841 atgacatgca caccaaaagg tgtcaactca caccgctttt tagtgtatgg tgaacgagtt
     3901 tccttcagta aagaagattt aaaactacag aaaaaagcaa aaaataggaa atcaatttca
     3961 ttggattggc gattagcact gctattcgct tttttttgcg ttcctattat ttttatttat
     4021 aaaaaaataa ggaggagatc ggaatgaata agagaaaatg gctgtttgtt tgtgtagcag
     4081 ctctcttttc tactgtagct tattcgcaac tgtctttcgc tgacagcagt gatagtagta
     4141 ccactcaaac aatagataat tcaattactg caccaagcga aacggacagt tcggagaata
     4201 ctacagaaga aacggcagat tcaaatataa gctttactga atcaacgaat ccaacgtcag
     4261 aaagttcttc agagacttca gaaaagccgc aagacgtaga agatacgcaa gtgatcaagc
     4321 aagccattca tattcaaaaa attatttctt cgaatcaaat caacaatcaa ggcagtcaag
     4381 tagcccagca agaaggtatc aatggcgcga agtttattgt ctacgatatc acgaatatct
     4441 tagaagaaat gtcacaagat gataatgaga aaaatcaatc tgtagaaata attgagtcaa
     4501 aattgaaaga tcgagcaaaa aaactttcat acgaccagtt gaaaaaagtg gctgaaggag
     4561 aaaccaaaac gattgatgat caggaaggcg tgattgaatt ttccattgaa gtacctgcaa
     4621 acaaaaaaca agcatactat atcgttaatg aatcaagccc tgaaaacatt tcaaacagtg
     4681 aagatattgt tttattgaca cctgtttcaa atgaaaaagg tgaatttttg acggatgttt
     4741 ggttataccc taaatcagag aaatcagagc cgaaagaaga aattaaaaaa gttgtgtcaa
     4801 ctggtgtaaa gaagaacttt tttgaaaatt gttgggaatt ttttgctcaa ttattttttg
     4861 ataattagga tgaaatgatg aaaataataa gaatattagt ttttaatcta tcctttttag
     4921 ccgcaacaat attgtgttgc ggtttaattt tttctcaatt atcaatttat ccaagatatc
     4981 aacgattgtt cattcatttt ctaaacattc aatgtgcttc catgccatta gaggtgatta
     5041 aaaagctctt gatcaatcca atttttatgg ttagtttggt tcttcttaca ggattggtaa
     5101 cgtgggtatt aggaaaaaaa gaatgaaaag cttacgatcg tttaaaaaaa tattagatgg
     5161 agggcattat ggaaaataaa attcattgca ctacgggaag agtaattact ttagtgttgt
     5221 ttttgttcct tcttcttttt gcgaggggaa ctgcagtcca ggccttagaa aaagttgatc
     5281 cgagtcaaat caaagaaaat aataatctgg tgctttatca tgatagtgat tcattgagaa
     5341 atatcgaagt aactgggatc aatggattta agatactaga tgagtattca actaaaaaac
     5401 aaaaagtact tgcttggcca aaagtcaaag atttgaaaag tacgtcaaaa atcgttgccc
     5461 attatcaaaa agtaggtact tatctaggaa aagaaattga ttgtgacgtg actttttcta
     5521 attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt aatggcaaaa
     5581 agtattataa ttatttgctc tttgataaaa atatttatcg aggatttttc atgcagtcaa
     5641 tcaatgtcat gaatgcaaaa ctagaattta agtatgcagg aacaaaaaca aaagtagctt
     5701 tgagtgaacc acagccagaa attcaatcaa aaatgagatc gatgagagca gctggtattg
     5761 atttaaacga tacatttcta tccatttaca gtttaaatgg acagtatcag caacgtgtgt
     5821 cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg agagaatttg
     5881 ttgggtatga aaaaatgggt agcttacctt attttgtcac aacagataca gcatgtgcag
     5941 aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg ggaggacgtg
     6001 cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct gatacttatc
     6061 acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg attggcgcaa
     6121 atacgaatag tatgatgttt agctttgata ctgcattgct atggacacca caaccatcga
     6181 agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac aatattgaca
     6241 aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag tttgatgcat
     6301 taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc tttgacagca
     6361 aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg tatgttgaag
     6421 cgaatggtac ttggaaaaaa gctgatacag gaggaaagtt aactgtatca ggtaatacag
     6481 tcacttatac tgcttctgac agctttttag ctaacgctgc aaattacggt agggattata
     6541 ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca aatgtggcta
     6601 ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataca gtagaaaact
     6661 atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt gatggtcgaa
     6721 atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa gtacaatatc
     6781 tagtcacaca aaaatggcat acaaaaggtg tagacattgc ctcagatcat tacaagcaat
     6841 ttagtattca agatccagtg gaagatcgtt tgacgtataa agaaggttct gcgcgagtga
     6901 tagatatgag tacaggaaaa gatattactt cagaaggtac actaacctat gatagcaatt
     6961 caagaacgct gaagtgggaa gcttccgctg atttcttaag taaaaatctt ttagatggac
     7021 gagaaattca gctgatattt acagctaaaa ctcctttgaa tgaagaattg gatatcgaca
     7081 accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta acgattgggg
     7141 taaaacctaa cttacctcag atcattgtgc caaaaactgg atcgaatcat ttagtgctaa
     7201 ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta gttttaaaac
     7261 tcaaataaat ataaaaacaa ggctgagaat cagccttgtt ttcttttatt tgaagattgg
     7321 attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa ctacaaaatt
     7381 atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat gggcagggct
     7441 atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt catcaatata
     7501 cagcccagtt tgacttatgt tttctccctt agcaccgtag cagcctaagc tttccaataa
     7561 atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta attctttagc
     7621 tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa caccattttg
     7681 ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac gtaattcgtc
     7741 taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt tattaatgga
     7801 tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca agaaataatt
     7861 ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata ctgtttttga
     7921 ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca cattaaattt
     7981 acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg ttactgaatt
     8041 tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag ttaaagtgac
     8101 actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc gcccctcatt
     8161 tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat tgaatgtgat
     8221 tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt tgtagctaaa
     8281 attatcttta gcaccttcgc tatatttaaa tgcttttgcc ttagcagtgt aagtagaacc
     8341 agaagtcact tttgcttgat ctgtagcaat cacttgacct gtttgattgt caatatggtt
     8401 aatggtaacc atatattctt ttggcgtttc aggtttgtgt tgcaaccctt taaatgcagc
     8461 atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat cgtctagtac
     8521 ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg taaaatctcc
     8581 tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat tcacagtagg
     8641 aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg cactatccac
     8701 ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta aagcggtttg
     8761 gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa cgctcgtata
     8821 aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc cataccaacc
     8881 gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt tccaacttgc
     8941 agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga tcggtttatt
     9001 atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat ttttagtcaa
     9061 ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt tattgtacaa
     9121 acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt cataatttcc
     9181 ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa aatgttcatt
     9241 attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa cagatccagc
     9301 aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat taaataattt
     9361 cattttttca actcctaaat ttaattaaaa atgtttacat gattattata tatcggctaa
     9421 ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt cttattttat
     9481 ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa tttcatagca
     9541 tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact tactgtagtt
     9601 aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc aaacagcaac
     9661 cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt ttctttatta
     9721 tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag aaaggtggat
     9781 ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt tcgttatttg
     9841 atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa ctatggtttg
     9901 tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat aagaaatcgg
     9961 aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa gaagtaggtg
    10021 gtcttattca taatttaaaa attatagatg atactacctg ggaatattct gaaagtgtat
    10081 ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa ggtttagaaa
    10141 gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct gattcattat
    10201 ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt ggatctagca
    10261 aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcagagtta aataaataca
    10321 aattacaaaa gactagtgag tgattgctag tctttttatt tatcctgaag gaggaatcac
    10381 agtggcaaaa gttaaaacac cattcgaaaa agccgcgtct aaaatcttag aacgccgtgg
    10441 tgtttctgaa atacagtgga aaaaagaagt tcttgaaaaa gcacaatttg acctattacg
    10501 aggaaaagat aaggacttag aagaatatat ctatcaacgt gaacgtgaaa aacttgtatt
    10561 cgatgaaatt attaaacatt aaaaggagcg aataatctta tgtttgaatc aattacaatg
    10621 aattttttaa ctcaaccgaa agtagtagct gcagttgata tttttagagc attacaaact
    10681 gccgcaacta gtttgaaaaa taatctgatg atttttgctg ctgttgcggc agtggcttcc
    10741 attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa cgggaagaca
    10801 cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc tattgtagtt
    10861 tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaaaca agtaggagga
    10921 gtaaaaagta cgtggctact ttttactcct ttttttatga aaggagaata ttcatgtttt
    10981 ttacgatttc aggaaatgca aaattggata aagagttaat aagaaaagca acgaagaaag
    11041 tgggaaagcg tacttccttt ttaccgaaaa caattaaata ttcggtcatt gaaacggaag
    11101 aacagctaca tgtgttagga caagtgttag aacaagagaa tcaatcttct gttaaattga
    11161 ctctcacgtt acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa tacgtttttg
    11221 gcccactgga ttatcaaatt tcagaaggtt tttatcctac attgatagat ttaatccgcc
    11281 ttgatgtgtt taaagatacg gatgaaacta tgagtgtgga agagtcttat cgaaacgaaa
    11341 aacttttaca agttttagaa gaagagttag agagcgacca aagaaaagtg aatcaagtga
    11401 gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc aatatttcca aagaaggagt
    11461 caaaagaaaa agagacagtc tatgttgacg aacaagctga tattccgact cagaaatcga
    11521 tatttgagga agagaacgat gtgatgggtc aattggaggt agatcccttt gaagaaacaa
    11581 tggaggatga agagacggac gaagtagaag ttgatccagt tgaagctaat gaagagtcaa
    11641 catggactga aggggatttt caggaatata ttgaaccact tatcagtgaa gaagtagaaa
    11701 aaaacgaaac agagatacaa aaggcaactg tatttgatta tattttggat caatatccgc
    11761 cagagcatga atggatgaaa gaaaaaaaca aattattttt aaatgagctt tattcgaaat
    11821 atcaattacc ccaaacctat caagcatttt tagagagtaa agaagaatta atctcacagg
    11881 gtaaaggcac acttttagaa cagttagagt ctgtccaatc aacaaattgg catgagcttg
    11941 gacaagaaga tttattagcc acctttgaag aacgtcaaca agaaacagat caagaaatta
    12001 atgagtatgc gacggaacag tcgtctaatt gggagaaagt aaaagaggaa ctaaataaag
    12061 aagaacagcg agtgattgaa gaagaagtac aaaaagtcaa agagcggtac gaaaagaaac
    12121 gcgacaatgc ttattccttg tgtgtagaaa agatcaacaa ttttactaag acgaatcaag
    12181 aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg tattaaagaa cgaaaagaag
    12241 agttctaccg cggtttacgt acagataagc taaagattag caaagaatta aatgaacaac
    12301 tgaataagct atatcaaaca acgactgaag ttcttatgga aaaacacgaa cagatccagc
    12361 aagaattaca agtacaaatt ccaaaatgga aaaaagagta tgaaattcaa caacaagaat
    12421 tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga gcgtgaacaa ttagaaataa
    12481 gaaaacggga acaggatctt gcggaacaaa aaatggcgat agaaaaacaa cggttagagc
    12541 aagaaaagga aaaagaagca gctgctcgta aggaacgtca agaacaaatg gatacattgc
    12601 gtcaagcaca gttgaattat ctagcacaac ctcaaccagt agctgctgtt caaacgccag
    12661 tgaacaacca agcaaattta actccttcac cccagcgttc ctttaacgga tggatggtgg
    12721 gatgtattgc aagtttaagc ttgcttctag gtggaggaac agtatttgct tttaatcata
    12781 tcaatcaatc ttcacaggct gattctgtag ccacaacggc gtcaatcaag gaagaagccc
    12841 aggaacaagc aagaaaagag tatgaacaac aattagaaga attattgaat cgtaccaatc
    12901 agtcaacaac agagtcaagc atggaaaata gcaaagagag tgattctagt aaggctaaag
    12961 aaggatcaag cgattcaact gtacaatcaa acacttccga aagttcagaa tcaaatgagg
    13021 tgaaataatg cgtgttagag aaacaacgaa gcgaaaagac aaacgattca atttagaaaa
    13081 gaataggtat ctttctcatt tctatcgcta tactccctca ggagaagagc taccagaaaa
    13141 acatgacaaa cgcatttcga aaaagatttt ttatagtgga cttgtatacc tccctttaga
    13201 gtatttagta acaagagtac cagaagtttt caaccgattg gaaattgtta agggaagctt
    13261 acaaggaata aatctgtttc aattacagga aatgccacag gtctttcatt ttgattcagc
    13321 tggtttttgg aatccattta ctcctacaac tagtttgggt acacgtttag caatttcgac
    13381 agtttgtgtg gttccagctg tacttatggg attgaaaatg gacatgtggt ttcgtcctct
    13441 agcagatgga caaaaagggg ataatcgatt aatgacctta aaagaaatcc aacagattta
    13501 tccgcaaata cctgaaacaa aaactacttt tccaggttat ggaggtattc caatcacaca
    13561 ttacaaaaaa tattggtaca ttcaaacaga tactgttaat acgtgtattg ttggtacctc
    13621 tcgatcaggg aaaggtcaga ttgaagtgtt agccaccatt gacaatttat ctcgtgcaga
    13681 aaaacaaagt tcaatggtcg ttaatgatcc aaaaacagaa ttatatgttg gtagtaaaga
    13741 tgaactagaa gctagagggt atgatgtata tgcctttaat atcaccgatc cactgcaatc
    13801 aatgagtgat aatccattgg cactcatcgt gaaatactgg aaacgaggag acatagatac
    13861 tgcaacacag ttaaccaata ctttcacaaa tacgatttat tatgatgcca acgcttcaga
    13921 taacaaatac tttaacgaaa acgcgcagaa agctgtcaat gcgcttattt ttgcgttgtt
    13981 agaacaagct gatcaaacag gagattactc caaattgaca ccgaataatc tcgttgagct
    14041 tctttctgag attggcggtt ttaattatta tccagatcca gacaatgaat tcaaacagtt
    14101 gaatgcctta gatgaattta tgaatcagtt gccacctgga aacattgcaa aaaaacaata
    14161 tggggcaacg aaaattggat ctgataaagc gaagggaaat attttatcaa cggcaatcac
    14221 gggattgaat ccatttactc taacgaaaat tgcaaagatg acttctcaat caacgattga
    14281 ttataaatcg gttgggtttc caaaatactt agatttgaaa ttacatgaaa gtcttttaaa
    14341 tcaacgagtc gtagtagaat tttatcataa gggaaagaaa tttcatgaag aacgtgtcaa
    14401 agtaggctat aaaggattta gtgaaatcaa ttttgattgt catttagttg caggtgatca
    14461 agtttgttta aaatacgatc atcaaggaaa agaatatgtg gcttggtatg aattttctca
    14521 aagagtattg aaagacgaag agggtaatat cgtttatcgg aaaaaacgag gagaaaccca
    14581 tttgccagaa ttagaaaaag aagccgttct aacattagat gaggaaaaaa gtaacttatt
    14641 agtagagtct attcggatgc actataccga taaaccaatg gctattttta tgcttacacc
    14701 tgattatgat ccatctaacc atatcattgt tagtattttt cttagtcagt tatataaaga
    14761 attatctacg caatgtgtta agactaaagg agataaatgt caccgcagga ttcattttat
    14821 tttagatgaa tttgggaata tgccaccaat ggataatatg gaaggaatca tgacggtcac
    14881 agctggccga aacatgttgt gggatctgtt tattcaatct tatcagcaac tttatgcgaa
    14941 gtatggagaa caaaacgcag cgattatcaa agaaaattgt cagacacata tttatattat
    15001 gtcgacagat gataatacca tcgaagaatt ttctaaaaaa gtggggaata agacggttga
    15061 acaagagaac agtaccatca attcattagg tatgaatact catatcaatc gcaatgttga
    15121 ttcagatcgt atcttaactc ctgaaagaat ttctactttt ttagaaggag aaacgattgt
    15181 actcgctcct ttaaaacgac aagatttgaa aggtcataaa atacggccgt atcctatttt
    15241 taatcatggg gaaacgaatc ttccttatgc ttatcaatat attgaagatt tcaatccatc
    15301 gtgggatatc aatgatatag acatacattg tacacatgcg gacttggatc ttaaggcttt
    15361 acaaattgat tatgattatt tcctaccaga tgaatatgct agaaatctct atcatggact
    15421 gcatgatcaa ttagaagaaa aaaacgaaga aaatatgttt tataaaatcg ttcaatctct
    15481 tgagaatgag acggctaaaa aattgttaat tcaattttac gaagaacaag cagcagatcg
    15541 acttcaagaa tatgttgaaa agttgatcaa aaataaagaa atttctgaaa tgattgggaa
    15601 acggttgtta ttggaatctg aaaaggtggt gaaagaatga tcattggagg aattttttct
    15661 ggcatagccg actggtggtc aggcttattc ggagaagggg atgcaaaccc caaaaccgta
    15721 acgaaagtct tacaagatta tgggaattat ctgcagtatc aagatgtatt tagttggatt
    15781 tggaacactt ttctgtgggg attgattaaa ggattatata atctcaatac tttactagaa
    15841 aaaaccattt atcaaagctt tgatttaaaa gacttattga acgccgctgg ggtaaatgca
    15901 ctatttcaaa gtttgattag taaggtattg gctctttttt gtgtcgtcac attaatttat
    15961 ttaggcttga aattttttat gagtaaacac ccaccaaaag tcaaaaatat cttggttaat
    16021 ttatttatgg ccatgatctt gatttttggt ggtagttctt taatcgatga agggttaaat
    16081 atcagtaaaa gcttttacgg agacgtcact gcagcaaata aagcagataa aacgggatca
    16141 cctgcttttc aaattattca aaataatgtg atcgatgtca gtacgttact agcaactgac
    16201 ccgaataaag tagaatccct accaacagat aaaagaaatg ctttaacagc gaaaaatttt
    16261 aaaacagcga atatcaatgg gattattact cctgaacaag ctaaaggtat ggcaggtaat
    16321 gataaaagtg ggttatcaga tatacaaaag gatcggttaa aggcattaca ataccgattg
    16381 gaactagatg ataatgggaa agaaattcct gttcaaatta gtgacgaagg cttagcaaaa
    16441 tatgtctata cttcagggta tcgtcgatac agtagtaaac caggtattat tttagctggt
    16501 gaatcttctt tggcagtagc ctatttattt atcctattta cgatcgttac ttgcattatt
    16561 gagctggtat ttaaaaaatt ctatttagtg attgccgcgt caacggactt tgagacaggt
    16621 caacggatga aaagtgcgat agaagatatt tctcaatcct tcttgttatt ggcgtttacg
    16681 attttagaat tacgaatcta taccttaatg ctttcaggta tcggtgattt gcatgcggca
    16741 ggaaagatca atggattctt gtatgtcgtt gctttgattg tactaaccat tgcgctattc
    16801 aaaggctctc aatcagttac gaaaattttc ggcgttgata caagcctgaa aaacggcagt
    16861 aattctcttc ttagcatgtt tgccttagga aatattgcga aaaatgtggg tagcggagct
    16921 aaaaaccttg caagtgctgg taagagtggt ttaagccatt taaatgatat tcgtaaaaat
    16981 ggatttggcc atcgaaaaga tgttacccct gataacgggg aagaacaatc agacgataaa
    17041 gctacaactg tacctaatag taagaaaaca gtttccgaaa tgttcgggaa agcgaaaaat
    17101 ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt aaaaggaaca
    17161 gctgaagatc ttacagaaaa agccaaagac accttagaag ataaattaga cggcacgaga
    17221 gctaaagaat tggctgaagc tgtgaagaat ccatccgaag cactagaaaa acaaaaagaa
    17281 aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca agtcaatgca
    17341 gcagtgaaag ctaatacaac aaccgaaaaa gaaaatcaag aaggtatagt gattccttca
    17401 gtcaaagatc accaagaagc gttgcactct aatgaaaatt cgattgaaga gaaaaattcc
    17461 atagaagaaa gaaattcaga agaaggaaaa aatggacagt taccaaaaac tgatccagaa
    17521 caatatcgct ataaacctaa cagtgacctt gaaaatgaga agcgaatttc agatttatca
    17581 aaagtaaatc aagaagacaa agcagaaaat gagggacagt taccaaaaac tgatccagaa
    17641 caatatcggt atagatctgt taactccgaa aatgaaaaac gcatttcaga tttaccaaaa
    17701 cgaaatcaag aagtacaaac ggatgaggat tccatcgttc tgccaaatga taatggaatc
    17761 gaagttggaa ctgaaaaatc aacgaaaatt ggtttaccta aggcacaaaa tttaccaatg
    17821 aaagatagtc gtttgaatca attcaaggat aatgatcaaa gttttgttca tcaatcacct
    17881 gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa atggaaatct gacaactgtt
    17941 tcttacaatt tatctgaaag tggtgagaca gggaatgcgt ccgttcaaaa tgttgtgaca
    18001 agagaagctg caccaaaacc tattcaaaac gttcatacag gttacaagcc agcaaatgtg
    18061 gcatttactg caccaacaaa aataaatgtg acaagagaaa caaaaaacag gaacaatgca
    18121 ttgaaagaac aactattgag ggatatcaaa caggatatca aaccataaaa ggcctacaag
    18181 ttaggccttt tttctatgta tgaaaaggag aaatcattta tggataaata tagtaacatg
    18241 aaaaaccctc caagatttag tggagaatgg aagtttttcg cttttttctt tttagatcta
    18301 ttaacgcttg gtggctgctt tttttgggcg aaaatggtca ataatcttgt ccacttacca
    18361 ctcttttttg cactcttgaa ttatggctta tggattgcga tagcgttagt gattgtttgg
    18421 cgaccctata ataacccagg tcaacgacaa atttttgcga ttatcgagtc tatttttttt
    18481 gcggacaaga accattatca tgcgttagat cctaaccgaa attatcatgg tacgtataag
    18541 gaggatgaat aaatgcgagt caaaggacaa aattcagttg aaaggaaatc cattttgcag
    18601 tttggaaaac cagaagagcg ctctatcttg gacttatttc catataaatc ctgtacagaa
    18661 gagggagtct taattactag agaagatcat tttcaacgtt attatcgtgt agtttctaca
    18721 gacgtggaag gattaaatga acaagagaaa ctagaacgaa tgaatcagtt gaccaccgtc
    18781 atgcgtacgt atgtgccaac aattaagtta gttagtttga ccacagagac ggatctaacg
    18841 gaacaaatcg tgcaaaagcg gcaattatta gataaaaatc gtatggaaca gtccttagga
    18901 agaaatctac aacgattaaa aaaatatgaa aagaaattag tcgaagaaat ccaggaattg
    18961 aaacgagcag aacaagaacg tcctgactta agcttctttt tcgtcattga aggaaagaca
    19021 atcaaggatt tacaaaccag aaaccgtcag ttaatgcgat ctagtggcat tttagggtta
    19081 aaaccactct acaaaaaaga attgatcaag attctgtatc gaatgaacaa tatgaatgat
    19141 gagtaggaat ggagaagaga gaatgaaata tccgttgata tggttagaaa aagatgttcc
    19201 gaaggaagag accactagaa aatttcctca cactttaagc ctgggtaagt tgggaaaagg
    19261 gcaatctttt aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagca
    19321 attaaaacag ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta
    19381 ggaaatcggg aaatagaaat tgtaaaatcc aaagggtatg atttagattt tttaaaacaa
    19441 gtgatgccac aaggtgggtt aaattttgat gaagaatatg tgacgtatgg taatggttat
    19501 tcacgatgtg tgacagttta tcactacaca aaaaatccta cgcttttttg gttggccaat
    19561 ctcatgaaca atcctcatac agtggccaca ctcgatgtta gcacagatga aaagcaaaaa
    19621 gtgattgatc aaatcaatcg tgccttagat gaattaaatg atcgatcaga taatgaacga
    19681 aaagcgactg accgaaataa aagcgcggaa gaatacctgg aactagcgga atatgctaga
    19741 aaattgactt ctaatgggga aatttctaag caagtcaaag tgagagtaca tgtttacgga
    19801 aacacgattc aagaagtaga tgaacgagcg caggaactga tcgaaaatct caaagggatg
    19861 gattatcgtg cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc
    19921 atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact accatcgtta
    19981 aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg agggacacaa
    20041 tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa aaatagtgta
    20101 cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt
    20161 aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga
    20221 aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct
    20281 gacggaatga tcaatccttt agagatattt gccacacgga cgtatgatga agcatccagt
    20341 ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaaca
    20401 cacttagaca aagtggtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg
    20461 aaagcagaat ttaagaccta cttaaatcgt ttttatgaag agtatggaac agtaccacgc
    20521 ggctttactt cctcaaattc gcgttcaaat actgatacgc aagtcacagg gaaagatcca
    20581 gaagcttatc cgacgttcaa agaattttta gactttttag gacaactgga attaccaggt
    20641 gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt
    20701 gagacgtatg gcatgatttt tgatggtcat agtacgattc gacaccttga ccaacaacag
    20761 ttggtctttt ttgacattga ctctattaca ggcttagatc gaggggtaca acaatgtcaa
    20821 atgtacatgg ctatgacgtt gatttggaat caggcattga ttcaaggaag aagacaacga
    20881 cgtttaatta aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt
    20941 gatgagtgcc ataatggtgc gacctgttct gcagtgaaaa gctgtgggtt gcaagtagtc
    21001 taaaaactat tgtaataact cttataacga gaagtcaaat gagtgggtaa cgccacaaag
    21061 ggcttccact gatactccga catgcaacaa gtagtttatg cagaccaacg ttgtgtgaag
    21121 ctcggtgaag tcggctgaag gataccgtaa ggtaaaagaa atagtgaata ccacgaaagt
    21181 tctctagagg taaagggtgt atccgataag tcgggtgtcc aaaaaaactt aatatggtga
    21241 gtatgtacaa acgtactgac gaactagcga atgtactgtc cgaaaggcat agctcctaga
    21301 aatgggagag acagtgagca aaaaatcact cattgtgcta acttgtggtt gagtaaaagt
    21361 cgttgttatg aaagaccatg ctgcgttaca ggcgtaggag aagttagggg gcaaaagcta
    21421 gcatctaagt taaggcatgt ggatagatat aagggaacag gggaagccat caacatggag
    21481 agtgcatact cgatgaagtg gtagtaagaa atatgtgaat gacaaataac ttactttagt
    21541 gatggtgaaa gtagagtcac gactgatgaa ggttctgtaa tggaactgga ggaacaggct
    21601 cgattcgtct gtaaaagtca tgaaacccgt tcaaccatgg gattctcgta tgactaaaaa
    21661 ggtcacgagc caattggagc gtgataaacc atgacaaaag aaacaagtcc aaaagatagg
    21721 aagttgcggc atgcagaata ttatggcatg acggaaatct tcgataagat atactccaaa
    21781 agtttgaagg gagataattt caagaatcta atgcagatta tcatttctga taacaacatt
    21841 gtcctagcat atcgtaatat caagagaaat cacggtagtg tgacaagagg agaagatgga
    21901 gtaacgatta aagatattga aaatctcaac cgtaatgaat tcgtagaaat tgttaggaag
    21961 cgattcgcac attataaccc tcggaaagta aggcgtgtag aaattcctaa atataatggt
    22021 aaattaagac cgttgggtat tccatcaata tgggatagaa tcgcgcagca atgcatctta
    22081 caagtattag aaccaatatg tgaagcaaaa ttcaataatc acagctatgg tttccgccca
    22141 aatagatcag cagaacacgc aatagcagat tgtatggccc gaatgaatca aagtcacatg
    22201 gaatatgtgg tagatgtaga tatcaaaggt ttcttcgacg aagtcaatca tactaaactc
    22261 atgagacaaa tctggtctat gggtatacga gacaaacagc ttctcttaat cattaggaaa
    22321 atgttaagag cacccgtagt acttccaaat gggaagttga tatacccaac aaaaggaaca
    22381 ccacaaggcg gaatacttag cccattactt gcgaacatca atctcaacga atttgactgg
    22441 tggatagcaa atcagtggga ggaaaaagat gccaaagagc taaaacatca ttttaggaaa
    22501 aatggagctt gggataaagg taatatctat cacaagctca gaaagagtac aactctaaaa
    22561 gaaatatacc tagtgagata cgcagatgat tttaaaattt tctgtagaaa ttataatgaa
    22621 gcgaaaaaag ttttctatgc aactaaaatg tggttacagg agcgtctaaa gttaccaata
    22681 tctgaagaaa agtcaaaaat cacaaatctt tcaaaagaag aaagtgaatt tttaggcttt
    22741 acgatgaaat tagtgaccaa aggtaacaaa aaggttgtta atacgcatat aggtacaaaa
    22801 gcacaaagca tgattaaagt gaaactaaaa aaacaaatca aacgaattca aaaaagtcct
    22861 aatagcaaag atacctttat ctatattgga gtctataaca gtatggttat tgggattcat
    22921 aattacttca aaattgcgac tcattgtagt gtagcaatgc gaaaaattgc aagagaactc
    22981 agaccaagtt ttgaaatacg actacgagaa caaggaatct caaaagaagg acaatacgga
    23041 aagtacgaag gatacaagcc ttacctacgg tcaggacaaa tacgatatat aagagggttt
    23101 ccaatattac cagtagggta tattcaacac agacatccga tgtgtaaaaa aagaaatatt
    23161 aataaataca ccgaagaagg tagggaagcc attcataaga agcaacaaac cgtagaagaa
    23221 tggaagatat cgtggttgtg tagcaatcca gtgcttagta agagagcaac gattgaattt
    23281 gcggacaatc gaatttcaaa attcatcgct caaaagggaa gatgtgcagt aacaggtgag
    23341 gagcttatct tatctgaaat gcattgtcat catattatcc cttatcatga aagcaaaagt
    23401 gatagctatg aaaacctagt gattgttact gaggaggttc atcgggtaat acacgctaca
    23461 caaagtgaaa cgattgagga acttctaaaa tatctaaaat taaatccaaa acaaaaagaa
    23521 aaactcaatg agttgagatt aaaagttggt aacgaagaaa tatcttaata aatatttgta
    23581 ggacgggaaa acagaatgtc ttttatagac gagggaacgc cgtatgcggt gaaagtcgca
    23641 cgtacggtgt ggactggggg aaaagacgga gataatatca aactcttacc tatcagtatc
    23701 gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg
    23761 taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa
    23821 tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat
    23881 ctttatgcgt atggatgaat ctattttacg tcatatagaa ggattagtgg gtaaatcaat
    23941 gacgaactca gaattagaat ccattcctca acaaaatcaa ggagacgctg tgatcaattt
    24001 tggctcaaaa gaaaccattc gtgttcattt tagtccgaac aaacgtcaat taaaattgtt
    24061 tgatggagga aaataatgga aaaccaaaat gaaagtttaa tcaagcagta tgttaagcgt
    24121 cgtgcaaagc ggcgcttatt tttgtggttg tttggtactt ctgcaggttt gattactatt
    24181 ttgatcactg tgttcgtgac cctatttcta atacttgcgg cgggctcgat tgataattcg
    24241 gattcagatt caagcagtgg tggcgaagct tttacaggag aatattcgga aggcttaccg
    24301 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgccgta
    24361 ggtgcagcag tgaaatataa attattacca agtgtgatcc tttcgcaata cggttacgaa
    24421 tcagcgtttg gtacatcggc gtccgcaaga aatgatctca actattttgg gattacttgg
    24481 tttgatggtt gtctgtttcc taaaggaact gcacgaggaa ttggtggcat tgaaggtggc
    24541 tggtacatga agtttcccaa ctcaaaagct gcattttcct attacggctt tatggtagcc
    24601 acacaatcca attttaatgc ttgtgtagga aataaaagcc ctggcgcaag cttattgatc
    24661 ttaggacgag gcggttatgc cgctgcagga attacggaag atagtccgta ttatacaggt
    24721 tgtatgtcaa ttatcacttc taataaatta accgaatatg atgaattcgc catcaaacat
    24781 tggggtgaag gtggcaataa caacggcacg attactggtg aatggacgaa tccattccct
    24841 ggcagcagtt tagataaaag ttccttcagc ggtggtcaat tatttggaac gaatccaggc
    24901 ggtgaattcc gtccgaacgg cttccatgat ggattagatt ttggttcagt tgatcatcca
    24961 ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt acgttggcaa tcctggtatt
    25021 tcaggtttgg gcgcgtgtgt gatcgtgatt aattacgatg gcttgaatat ggtgtatcaa
    25081 gaatttgcaa atagtacagg aaattctcga gtgaaagtag gcgatcaagt gaaagttggt
    25141 caagtaattg ggatacgaga tacagcacac ttgcatttag gatttactcg aatggattgg
    25201 cgtcaagcac aaggacatgc attcattgat gatggtacat ggatcgatcc gttaccattt
    25261 ttgaatagca gtaaaaaata gatcaggaga aaaaatatgg aaaataaaac gttaaaaatc
    25321 gtcttaacag ctgcaattag cgttagttta atcggcaata ttgcgttgtt ttggcagctg
    25381 aaagaggaac aagcagcaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgag
    25441 aagctcaaaa aaaattcctc tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc
    25501 aatgagaaaa accaagaaat cgatcagctg aagcaacaag atggcacaaa agcaaaagag
    25561 gaagtaactg aagcacaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
    25621 tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
    25681 gacaaattaa gtcctacaga cgatgatcac caacatgatg attacggaag ttactccttt
    25741 acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga acaaaatttt
    25801 gtcgtatttg ttgattatac aatcggcaat ccgcaattca aagatgtcaa accacaaaaa
    25861 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcatagac
    25921 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag aaagacaacg
    25981 tttcaaaaaa cgaaaaagtt atcttagatg gctgggaatc tttatgattc tcggcttttt
    26041 gattcttgct agtccgattt atacgaatca gaaggtagat ataccagatg aggtaagcac
    26101 gaacgccttt ttaaacacga aagagctgac aatggtttca aaagaaaaag gagcgaacca
    26161 gcaattagtt tttgaatttg cgatcgatga tgaagatcaa agcttgttaa aagaactggc
    26221 taatcttaat tatcgcgtag aagtaaagac agctaaaggg gattatcaag caatacgagc
    26281 caaagtgatt aaggtgtctg atgattattt tgcagtgaag ctaacgcatg taccagaaga
    26341 atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt gatccaaaag tggatatgca
    26401 agaaccacaa gatctgattt attacgtgca cgaagataag gttaaagatc aagtgaaaga
    26461 tgaagattat gaacaacacg cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa
    26521 agcagctcaa aaacaaatgg aatctttcca agcaaccatt gatttgaatg aagaattagt
    26581 gaagaagcta aaagatcaat tgccttatca agtcgcagat gatcaggaaa atactgaaaa
    26641 caagattaat agctaccagc aagaaattga aacatcgaaa acgcaactga aagatcaaga
    26701 agagatcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa agatttagaa
    26761 ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct gacaaagata ctttcgctga
    26821 gcagaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa tatgtcgaga
    26881 agtgaattta tctaacacaa caatatctga gttgaagaat ggtcgaaaga atttagatac
    26941 tttaaaattc agatcatttg aagctctata tgtagctgct aaaaaatatt ttacagctga
    27001 taacaaataa aaaacaggag tattttatgt gaaaaaagta tataaaaaat gcttatgaat
    27061 cagctatatc accttaacca tttgttaatg agatgggaga aaacattaac cacgattcta
    27121 acgcaaacat caagcacata aaacatttaa cactcctcct ttctggctga agatttcaac
    27181 caaacaggaa gacagcgtat caattatagc atacactttt tcttaaattc atttttcata
    27241 tgacaaaata tttccttcta atcgacaaag ttagaaggat tttttattgt ctaaatacac
    27301 aaataacctt aatttttaaa gagcataagg aaacgtgata tcactttctc acgaaaggtg
    27361 ttaggtaggc tgtgcctacc acgtttctaa gaaagataaa tgatatcaaa attttggatt
    27421 tgtgtgtaga tttgtgggta ggatgaaagc gaatgtgggc agtaaaataa gcgtttgtgt
    27481 gtagcaaaat caaaaaatgt gtagggatat gtgtagctac acatatccct acacataata
    27541 aatttggagg aaaatatgga cttattaatt agaaatattg aagaaaagta tattaataaa
    27601 attgacaaac gttgtgaaga acttacggtc aagactggca aaaaatggag tcgaaatcaa
    27661 tacctaaaag ttttgatcga aaatgatttt gatcacgctc ttctgaatta taaaaaagac
    27721 caatttgatc ggttgttaga aaaatttgtg gatattcaaa cttataatac gaaaacttta
    27781 gaagaatata ttgcaacaaa taatcaatta attggtttgt taattgaata ataaaaatag
    27841 tattgtgaat ttataaaagg aggaaatcaa ttttggcaaa agcaacaaga aaacatgtat
    27901 atctttacga tatgcatatc gaagtacttg atgaaataat ttctactcat gaagatatat
    27961 ctaattatag tgaagcagtc agacatttgt gtatgatgta tgatccaaaa atcgaagaaa
    28021 aaataaatcc aattcaaatt aaattgaatg caatgggaaa agaaatttct attttaaccg
    28081 aaatggtttc agagtttgcg gatcttcatt tgaaagatac aggaatacta attggtacaa
    28141 attcaactgt ttatcaagaa gcagaagaac gtgtggaagg aaaaattaaa tccaatcaaa
    28201 ccaaaaaata ttcttctaag ttaaagaaac cagtgaatga atctgttcat accagagtag
    28261 atccatttga tccaacgaag attctaaaaa gataaaagta gaaggagaac gtgatgaata
    28321 aatcgcctgc agttattctt acttgtcaat ttaccttgcc caataaaaaa tctttttcaa
    28381 catacattga ttatatgaca agagaaaaag cactggaaga aatggaacga cgtacgcccg
    28441 aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat attcctgaag
    28501 gagaaacttt ttcggaaagt aagacggata aagaattcag tgaaacgtcg aatgaagctc
    28561 aatcgttgat ggaacatgga atcaattttg acaagttaga ggaacaagat tttaccaaat
    28621 atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg acgcaaaggg
    28681 aacaaaaaga ataccaacgg ttgaaccaag caatgaaaaa atatcaaaca gagccacata
    28741 aaaaagcttc aggaaatctt cctggagttt tttctatggc ttcagacgaa gtgaaaggaa
    28801 gtgatctgaa ggagatcaaa caaatcttcc aaagaggaca acaaaaaggc tcaatcattt
    28861 atcaagatgt tatctctcac gacaattcgt atcttgaaaa aatgggcttg tacgatccca
    28921 aaacagatac cttagatgaa gaaggtttga aagcagctgg aagaaaaatg atggagacat
    28981 tattcgagaa agaacaattg aatgaaacag gttactggat ggcaacgatt caccgtaaca
    29041 ctaagcatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt aaaccgtata
    29101 ctgagattga aaatggtgtg agttatatcg taccgaaggg gaagagaaga caagcaacat
    29161 tagacgcaat gaaaaccaat tatgtacatg agttagaacg tttctcttat gaaaaggatc
    29221 gaatgatcgg tatggagaaa cgaaatttat tgacacgaaa aagtgatttg cgtaacacct
    29281 taacaaaagt agtaaaagaa ccagcacgtt acgatcgaca cgccatgaac ttattaaatg
    29341 aagtatataa aaccctacct gaaaaacgtt cggaatggaa ttatggagat gaaaatcgaa
    29401 ccaagctttc accatttaca aggggaaaac ttgatgagtt aaccaaatat gttttaacaa
    29461 aagaacctgg atatcaagaa tatatacaga tttcaaaagc attgaaagaa gaagccaaaa
    29521 gcctttatgg ggaaagcaaa agagaaacaa agaatgcaga aaaaaacgcc ctctttgatt
    29581 taaaaaagcg cacaggaaat gcgattttaa cagaactgaa gagacaggat agtcaaataa
    29641 aaccattaat agaaagtgtc aatcagatgc aatctattta caatgaacga tcctttcgaa
    29701 cactcaaaca gttgccaaca aatgaataca agtatagtta tcaagaattc gtaaagcgta
    29761 ggaaacaatt ccaacaaaga gttgtcacgc agcgaagctt gcgtcaatta aatcgaacaa
    29821 ttaatgcaca tgaagaaaaa taccacgcaa gtaaagcata tgaagagcaa cagcgaagaa
    29881 tccaaatgga acaagaaaga caataggagg aagagaaatg aaacaatatt tagatcaatg
    29941 gaaagtcata gaaggatcac taaaagaaga acgaatcgaa caacttcctg attgtttaga
    30001 aaaagagcat ttgtttcaga taagcgaaat gttgagaaac gaacagtttg atccaaatca
    30061 atttttagtt gttgagtacc cagctactgg tgtgtattgt tgtaatcatg tgaagggtga
    30121 aaaatatttt attattcaag aatacgaagg taagttagcg ccttattata ctacttggga
    30181 aataaatgaa gagggaatca ataatttccc atgtgaaagc attgaagagt ctatttctct
    30241 cacggaatgt taaataaata gagacgtaaa agagtcctga acgtgattgt ccaggactct
    30301 ttttcaatga attgttttcc gccaattcac tgagtggtgc gattcaattt tattatatca
    30361 aaagaaagtg agattccaaa gtgaagggag gaagaagaaa tgactcagac aacgattggt
    30421 gttgtggttg aaaaaagcag ggataatctc gtttttgtga cagaaattca aacaggtaga
    30481 gcttttgtag tcacagacaa agctgcaaaa gcgtatcaaa atggcaatat tttaacgtta
    30541 aatctgacta cgaagatgtt cgttgatgca gtagaaaatt atccatttgt ttagacaaac
    30601 gatattttac aaggaggaaa cggacatggc agaagaaaaa gaaccactag ataatagtcg
    30661 gttgggaaag tccaaaagaa aattagtacg gttgcaaaac gaattaaata atcaatttca
    30721 aaagctattt gatcaccaaa aattgacaaa tgggcaacca atgaatgaca aacgaaatgg
    30781 tcgttcatgg tttaaccagc aagaaaaaat agaaaataaa atacaaagtt tgagagaaga
    30841 gatcaagcaa caagaaaaac aggtagaaaa acttgaaaga caagaagagc taaaggaaat
    30901 gggctataac aagtacggcg gactggatat gacgatcgat aatattccaa gaatcaaaga
    30961 agaaatcgaa cgctttgaaa aaggtgaatc gactttttca gccgccacga tccgaaaata
    31021 tcaacggaag ttggaaacat tagaacagct gaaagaaaaa tcagaaaaag gaaaagaaaa
    31081 tatcctccct gaagtacaag cgatcattga cagcggacga gtgacgcaat ggaaaaaaaa
    31141 tccaaccatt tattttttga aaggctatcg aaaagttgct ttagaactga atgaaaacgg
    31201 gggatttgag gaatcaaaaa aataccaagc cacgtcggaa gaggaaaaag cagctgttcg
    31261 tgaattactt gaagaggctg caccaactga ttctctgaag gagaaagaaa aagatcattt
    31321 ctatcgagta gaattcaacg aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga
    31381 gatcgtaaca ttagaattga tcgatcaact taaaacgttg gataaccaat acgctgcaga
    31441 agcaggttat tataaattct attttgaaga agtgaagaac ggagaagtag tagatcagat
    31501 ccgaatggat ctaggggatg gcgtcaacag taatcacccc atctatttag agttagcaaa
    31561 attgtgcgaa caagtacctg agaaggaaca aaaacaatcg gtcaaaaatg aacctgtttc
    31621 gaaagaacaa atgcagtcga aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac
    31681 ggcagaagag atcaaacaag aagttaaatc cctttcgcaa caggcactgg atgcggtgaa
    31741 aaagcatacc cattcttctg aagacgtcaa agaacttttg aactttatgt ctcgttttcc
    31801 agaacgatcc tttagaaatc aattattgat tgaggaacaa tttccaggtg caacggcttg
    31861 tttaggtcgt gccgcgttga aaaaagaagg aatctatatc aaaaaagggg aacaagggaa
    31921 caagatcttt gtcagaaaaa cggtcaaagg attttatgag aaaggacgtg gctttgttcg
    31981 agaaacagaa gctacaccag aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat
    32041 caaaaaacct tactacacga ttgaaaaagt gtttgatatc acacaaacac agttaaaacc
    32101 tgaagactat ccgaaaatct ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg
    32161 acagtcagaa cttcaagctg ggattcaagc ggtagccaac aatattggaa tacaaattcg
    32221 agatatgaca gaaagtgaag tctatcaaag agaattagga caagcacgtg gggcgtatgt
    32281 tcgtaacgaa tttactggcg aagaagaaat cgtcatgaat actcgaaata ctcctacgca
    32341 gcatttagct acaagtattc atgaattagc ccatgcaaga atgcacaaat tttcagaatt
    32401 agataccgca actaaagaat tgcaggcaga aatgacttct tatattgtgt gcaaacattt
    32461 tggaatggac acgtcggaga aagctattcc ttatattgca gcatggacga agaatggtca
    32521 gacgttggat gataaggaag cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt
    32581 cgcaaatgaa ttcattcaaa ccatttctaa tgaaatcaat caatacagag aaagagaacc
    32641 agagatccaa ccagcagaag tgaagcagga gcataaaaat actgtagaaa cggcagagaa
    32701 taagaatatt gtgcccgttg gttcactttg gatcgacaag aaagacggga aagtaatgga
    32761 agaagatacg ggtcgaacgt tacatttgaa ggatgcggcg tttattgaag atccgcagca
    32821 taatcaagtg attttaaaag gaaaaagtta tggaaaagag attcaaacgg cgttaccttc
    32881 agaagaattt gcgctaggaa atccattccg attttcactt gaaacagtgt cagaagtggc
    32941 caaacgaaaa aatgaaatct attatgaaca acaattgtca caaaaagaac gcgtataaaa
    33001 caagtaaaaa agactatctg aaaaacggat agtctttttt cttttgggag gtttgaaaat
    33061 gaccgaaaaa aaaatgagtc ttattgatcg atgtaaacag atcgatatag tggattttgc
    33121 tcgaaacaat ggaatggctg tagtcaataa aggacgagac tatcgcttag aagaccatga
    33181 ttcatttgtt tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg
    33241 cgacattatt gaattagcta aacttttttt catagacaaa gagatccaag actctaagca
    33301 acaatttaaa gcagccactg attttattct aaagaatgaa gacaaaacag aacgagtgga
    33361 aaatcttcat tttgaaacag aaaagtacaa agatcacccc gttgattatc aaccactcac
    33421 agaaaaagga aggaattatc tgaaggaaga acgaaaacta ccagagtggc tgattgatta
    33481 tgcagaaaaa gaagggttga ttgccgaact aaagccaaaa tatgaacggc aaaatttctt
    33541 agttcgagac gatcgtttag atcatgcggt cgccttttta tggaaagatc cacagacaaa
    33601 agagacagta ggggcgagtt atcaaggaac gttcattgat tatgaacgtt ttggtgagag
    33661 aggcacatat aagcatatag ataaaaattc gacagcgaat catggcttta acctaaagat
    33721 cggtgaccct aagcagttaa aattttttga gagtagtatt gatttactta gctatgctgc
    33781 actgaatcgt gatcagttaa acgatacgtg gttagtctct atggaagggt taaaacacca
    33841 tgtgatcagt cattattttg gtgaggcagt atctgaattg agaaagaaac aagcctttcc
    33901 tcaatccatc gagatttgtg tggataatga ccgtgcagga catattttct acgaaaaaga
    33961 acaattaatg ggcgcagtcg atccatttac gaatcagaaa gtacgttgtg aacgaggaat
    34021 cgctaatgat tggcaagtgc caaaagaata caaaatcatt tatgaagaag tcgcaaaaga
    34081 agaaaaggtc acaccagaag ctattatggc catacacaag acagaaaaca atctgcaatt
    34141 aacgaaccaa ctggtttccg ctcacaaagt aaatgcgttt tttggccagc agctttctgt
    34201 taacgattca atagaagcca ttaatttgaa agatatttgt agagaagtcg caaaagaatt
    34261 aaaagtttgt gaacgcgtgg atggtacgta cgattttgat cgtttttatc aagaaaaagg
    34321 ggatattaat gcgcagattc ttttctctta taaagcagaa caatactaca aaggctataa
    34381 aaatcatgaa catgaattta tacctgaagt aaaaaaggac tggaacgatc aattgaagca
    34441 tgagatccag caacaagaaa tcagaaagca aaaacgtgcc atgttgtttc aacaaggcag
    34501 acaacaagaa agggagtgaa atcgtcatga tgaaaaccgt tattttagca gaaaagccgt
    34561 cacaagcgaa agcttatgcc gatagcttct ctaaagccac tcgaaaagac gggtattttg
    34621 aaatccaaga tcgattgttt cctggtgaaa ctgtgatcac gtatggtttt ggtcatttag
    34681 tcgaattaga ttcgcccgat atgtacgatg aaaattggaa acaatggtca ttagaacatt
    34741 tgcctatttt ccctacacat tatcactatc atgttcctaa agataagaaa aagcaattca
    34801 aggtggtgaa acagcaactt caatcggccg acaccattat tattgcgaca gatagcgatc
    34861 gtgaagggga actgatcgct tggacgatca ttcaacaagt aggcgccgat caaggaaaaa
    34921 cctttaaaag actttggatc aactccttag aaaaagaagc catttatcag ggattccagc
    34981 agttacgaga cgcaaaagag acctatccaa aatttgaaga agcacaagca cgtcagatcg
    35041 ccgattggtt aattgggatg aacggtagcc cactgtatag tttactcttg cagcaaaaag
    35101 gcataccagg aagcttttcc ttaggcagag tccaaacccc aacgctttac atgatttatc
    35161 aattgcagga aaagattaga aatttcaaga aagaacctta ttttgaagga aaatccgaag
    35221 taatcgctca aaatggcgca tttgatgcga agcttgatcc gaatgaaacc caagcgacgc
    35281 aagaagcatt tgaagattat ttaaaagaaa agggggttca attgggaaaa cagccaggca
    35341 tgattcgcca agttgaaacc gagaaaaaaa gcgcggcaag tccacgtctt ttttctctat
    35401 ccagccttca gtctaaaatg aatcagctca tgaaagctag tgccaaagac actttggaag
    35461 ccatgcaagg cttgtatgaa gggaaatatc taagctatcc acgaacagac accccttata
    35521 ttacggaagg ggagtatgcg tatttgttgg atcatttaga tgaatacaag cattttttaa
    35581 aggccgaatc aatcccaacc cctattcata cgccgaatag tcggtatgtg aataacaaga
    35641 aagtccagga acattacgcg atcattccta caaaaacggt tatgacagca gcggcttttg
    35701 aacaactaag tccactgcag caggccattt atgaacaagt gttaaaaaca acggtggcca
    35761 tgtttgcgga aaaatatacc tatgaagaaa caacgatctt gacgcaagtc cagcagcttc
    35821 agttaaaggc aatcggaaaa gtaccgatag atctaggttg gaagaagtta tttggcaaag
    35881 agaacgacgg aaaagagaag gaagaagagc cgctacttcc taaagtgaca aaaggagaaa
    35941 tggtgacggt agatctacaa gtccttgaaa aagaaaccaa gccaccacag ccttataccg
    36001 aaggtacgtt gatcacggcg atgaaaacag ctgggaaaac cgttgatagt gaagaagcgc
    36061 aatccatatt gaaagaagtg gaaggaatcg gtacagaagc gacacgggcg aatattattg
    36121 aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa taagctcgtg gtgacgaata
    36181 aagggatttt gctttgccaa gccgtggaaa aagagccgct gcttacgagt gccgaaatga
    36241 ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg aaaaggaaca caaaccactt
    36301 ttctagccaa tatccaaaaa tttgtctctc acttattgga agtcgtacca gggcaaatcc
    36361 aatccactga ttttggatcg acgttacaag aagtgaaagc agccagtgaa aagcaagaag
    36421 ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca agaagtctta ctctatcaaa
    36481 aggtggcagc atgtacgtct gaagcctgtg atttcaaact atggacaacg attgccaaaa
    36541 agaaacttac agcgacacag ctgaaagaga tcattcaaaa tagccgaaca agccaaccag
    36601 tgaaagggtt aaaaggtcag aagggttcgt ttgaagcaac gattgtttta aaagaagact
    36661 tcactacagg ctttgaattt tctgaaaaga aaaaaacgaa cttcaagaaa agaacacgac
    36721 gcactacaaa ataatggagg gttaatctta tgcgtaatac aacacacaaa caacaagaaa
    36781 tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa aatgcgtgcc agcaaacgaa
    36841 gactgcagca attgaagaac cagcagcgga ttcaaaaaga aatcgaatta gatcgataaa
    36901 ttcctttctt gttaatataa aactaacatg ttatagttgt gttagtttta tattaacttt
    36961 aggggaagag taatgaaaga caaaatccaa tgtatttttg ataaacataa tggcaaccta
    37021 tccttgaagg atgctagaag tgaaggatta tcgcctatga cgttagatcg attagccgaa
    37081 aaggataaaa tagaaaaggt agcaccaggt ttctatacct taaaggatgc gttcgtggat
    37141 gaattgtatc tcgctcagag tatctattcc agagggattt tttctcatga gactgcgctc
    37201 gatctttttc aagtaggaac atatatccct aaaaagatca atatgatgtt tcctaaagga
    37261 tacaatgtat caaaggagaa attagatcag tatgccattc aaccgcatta cactaataaa
    37321 gaaaattttg acctgggagt cacagaaaca gaaagctttt acggcaataa aattttagtc
    37381 tatgatttag agcgaacgtt atgtgacatg tggaatccaa gatacaaagc aagcgtagag
    37441 gtaaaacaag aagcattaaa agaatatatg acaagtacca gtagaaatac aaataagcta
    37501 agaagataca tggatatttt aaaatcacca aaagaaatga ctttgtatat gttgccactt
    37561 tattgagagg agtaaagatg aatgacaccg acacaattta aagagaaaac aagaaatttc
    37621 gcagcagaga aacatttaaa cccacaaatt gtccaaagat attatatgat ggaaaaatta
    37681 ttagaatgta ttgttgaatc gccttatcat gatgattttg tattaaaagg gggatttctg
    37741 attggatcaa aatacgggtt agaaaaccgt tcaaccgttg atattgatac gacttatcga
    37801 aattcgaaac taacaaaaga aaagttagaa actgtttttt ctgaattaac agctaatcct
    37861 acaaaagatg gaattcgttt tgaattaaag ggattaacag agacgaggga agccgattat
    37921 tatccaggtt ttcaagcaaa gtttattgct tatcttgaaa atgcacgtgt accttttaaa
    37981 ttagatatta cttcaggtga ttctattatt ccagcagcat tgatgtatga acacaaatta
    38041 atgtttgaag ataaaacgat tcatattcca gcttatccta cggaacaaat tctagctgaa
    38101 aaattacatg ccacgtttag ttttggaaaa gataattcgc gcatgaaaga tttttatgac
    38161 ctgtatatga ttccgaaatt agaacgtatc gatgagaaaa atttgtataa gagtgttcaa
    38221 ggaacattca aggaaagaaa taatcaatcg gtgtttaagc atttgtatga tcaggaaatg
    38281 cctcaattgg tgaaagatga gggcttgaga gaaaagtgga aaagatacca acaagaaaac
    38341 tattttgcca aagacatttc tttcgaatct acactaactt ccgttgattc cctgatgcaa
    38401 tcaattgtag agcaagagaa ggcagaacga aaacaaaatt ttaagagatc acaacaaatg
    38461 aatcaagaaa tggagagata aagatgatca ataatgtaac tttagtaggg cgattaacta
    38521 aagatccaga tttacgttat accgcaagtg gcacagcagt ggcgactttt tccctagccg
    38581 ttaatcgtaa tttcacgaat caaaacggca atcgagaagc tgattttatc aattgcgtga
    38641 tttggcgcaa accagcagaa acgatggcga cattagctaa aaaaggcagc ttgatcggcg
    38701 ttgtcggacg aattcaaaca cggacttatg acaaccagca aggacaacgt gtgtatgtga
    38761 cagaagttgt ggccgataac ttccaactat tagaaagtaa agcagcgaca gaaagccgta
    38821 tgcacgctga tcaatcgtcc agcagtccaa gccctaccac ctttgaacaa cgggatacag
    38881 cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga caatcaatcg
    38941 atattagcga tgatgatcta ccgttctaag tagcattttc tattggttgc atttgctacg
    39001 gtttatgtta taatttagac aacaaaaaga attttcaatc tataaccaaa aaccactagc
    39061 tggcgggcta gtggctttct tttacgtttc tattataaga attaacgggt aggcttactg
    39121 tctttcttgg caaatttaca atcaagccat ttgcagatat agtgcgcggc tacttctgcc
    39181 atgattgcaa aaacaaaatc aaaaagaatt ttcaaaccta taacctcctc cccttgccag
    39241 attaggccgg ggtagcagac agcaagacta ttatagcata attgaaatat ccaaatgatt
    39301 ggatttttgc tataatttta gcgaggtgat tgtgatgaac aaatatgttg gtttgcttga
    39361 taaaatccga gtaataaaaa cgcaaccgtt attggttcgc tttactttac aaacgattca
    39421 tgaatcgatt aattgtgtag ttgcagatat tgaaattatc gataaattgc taattatgga
    39481 tgatggaaaa tacaatattg ccgtcacagg tcacttcaat aaacgaaatc agctagttat
    39541 tgctagcatg tatgttagaa atcccgatca ttttacgcga tcgatgggca tataaataaa
    39601 agaagagttg tagtcctaaa tgaggattgc agctcttttt gttttacata gaaacaggaa
    39661 aagacctttt taggaagaca caagtgcctt ctcaattgaa aaacctttct ctcactccaa
    39721 ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca cgttcactaa gatatggtgt
    39781 cttgcaaagc aagacaaaat agacaaaact tcctctcacc aaaaggttcg ttcctgttcg
    39841 ttatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc aaaaagcgac
    39901 aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc gttgatttat
    39961 tctggccaac aaatcaataa agaaagaact aacgaaagga agtaaaacct ttcctaagag
    40021 gaggcagaat caaacatgcg aaagaaattt gattattggg gtgttccctt cagcgaatta
    40081 ttcccaaact atcatgcacc acatactgtt gaatgcgatt gtggagaaag agcgaaatgt
    40141 atcaaaagct atcgtttgta tcaatgtcca acatgcggga aaaaatatac cttgtcttat
    40201 ggcgattaca ttttgattga tgagaaagga aagaaacgct gatgaaaaca caacacaaat
    40261 tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt tctgaaaaag
    40321 tagcagaagc tttacttatt ccgcggtact ggacgataga aaccccttta gtagaggatg
    40381 caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg cattatcaag
    40441 aaaacatgga aacttatgaa atcgaatatt ggttaaccaa attagagaac taaaaaggag
    40501 aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc
    40561 acaaaaaatg gaggatatga cgttcctata ccatgaaaca cgtgtaccca aaaaacatta
    40621 caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa tcaggtgtcg aaatgaatct
    40681 cattgcgacg tactatcgaa cactggaaga actgaaaaaa caaaatggaa aatggttttt
    40741 ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actatcaaac cttccgaata
    40801 tcaagcctta gaactgactt atgcaaaatt cgcacagatt aagaaagcta aagcaatggc
    40861 tagcgactgg ctagatactt ttgaaagtat cgaaaaaaat ggtgcatttt atacgattga
    40921 atcggaggag aaagataatg aataaggtgg tttatcagct aacaacagaa gatgtttcgc
    40981 aatttttaac aagcaaagaa cacttgaatg aagtaatcag acggattcaa aaagacttta
    41041 gttgtatgga agaaatgaag tactttattc aaagagtgga agaacagttg tatatggaaa
    41101 gcgaaacaag tcaaacgaag gtcacagtca gccataatca tttagacgtg tataaacgtc
    41161 gtcctgaaac ggtaaaaaaa ggaacagagc ttctacgact agaaacagaa aaatacatca
    41221 tgaatgtgta cctagaaagg agtgagctgg tcgaaaactt tgtcagctac acgatttgta
    41281 ggaaacacta ttactcagaa tcagaatatt attcaatttt cgagagcatg gaattaatgg
    41341 aagaagaaat ggaagagcta aacagcaaaa atgcgacggt agacgattat taccttgtat
    41401 ttactaggtt tttgacgtgt gggacggaat ctatcgtgtt aatgggaatg gattatgaac
    41461 tcgctgtacc cgaagcattt ttgagggaat atgtagaaga agtagataac ccaggcgacg
    41521 actggtacac gactttaact gattttttag acacctacac ttacgacgat acagaagaaa
    41581 tccaaaatga acttgaccgt aaacgaatgc catatcaacg atttgaaagg aaaaaaggta
    41641 aataaccatt tataccgaaa agtgaggatg tgtgaaataa gaacaaaaaa ccttgaaatt
    41701 aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc ccccgccgct taggtggtgc
    41761 gaaataagcg ggcaaagccc gataacctaa gagaaagaag gagatccata tgaataaaat
    41821 cattcctcta ttgttcgtac tgtttgttgg cgagatactt atgcgtcatg ctgcagctgt
    41881 acatttaaac ttcgtagtat tcttaggaat actggtattt tgtttgatct tttggcgatt
    41941 tgtattagcc cttgtgagtc tactattcaa gatgattgca gccatatttt tcatagggtt
    42001 atttcgatga aaggagagag tactaatgcc aattgcaata ggaaataaac ggttacccgt
    42061 gacattagat gaaaagagac aaaaagaatt gcagcaacta aagcagaagt acggaaaaag
    42121 tgaatctaag attatgtgta ttgcgttaga tctattgatc gcgcaagaaa aagcaggatt
    42181 tgatatacca gcactaaaaa agtgacgtca cttttcattt gaagaaacaa aagtgatagc
    42241 acttaattgt tcctactatt tagaatattt tctatgtcct ttttattcca ttttgtatca
    42301 ataactatga aatctttaaa gtatccagaa gagattattt ttagatcaat ttttttctta
    42361 cctgattcta aagattttgt tttcttaaac atattatgat ttcctttcga ctattttcat
    42421 ataaatgtta acatagttta tggattcatg gaatgttaat ctaatttaat agaaaaggat
    42481 aattaagtat atacgttttt tcttatgtat gttatactat gtatatacag aaagaggtgt
    42541 ctactatgga aatgttacct ataaaaaaat ggggaaatag taatggcctt agattgccaa
    42601 agtatattat ggaatatctt ggaattcata cagaagacaa agtgaagata atacaagaag
    42661 aagtaaacgg tcagaagcgg ctaattatcg aagcggctaa tatagaaaat gaattaacaa
    42721 ttgaacagtt atttgaaaat tacactgatg aaagaaatca tgtgacgatt caaaatttag
    42781 gtgaagcagt aggtaatgaa aaatggtgag aaaacctaga caaggcgata ttctattatt
    42841 gaatacagca cctcgatctg gacacgaaca gacaggtaaa agaccttata ttgttttaag
    42901 tcatgatatt attgcggatt atagtaacgt tgttacagtt gctccaattt ctagtacaac
    42961 tagaaactat ccgctatatg taaatataaa ctcagaatat gaaatgaaga caacaggaaa
    43021 agtattatta gatcaactaa cgacaattga ttatgaagcc agagagtgta tttttttgga
    43081 aaaagcgcat gatagtttag ttgaggagtt attgatgaaa gttagaacag tttttcaaaa
    43141 agtaagcaaa taaaatagaa acagcagcat gatcaactat tcgtgattga ccatgctgtc
    43201 tttttttatt ctatatacgg tttgtctcgt aacattgtag tcttttgcaa tcttagcgat
    43261 cgcaacacct ttttttagat cctcaacgat gtttttataa acaagacgac gttggggatc
    43321 ttttgcattg gcactgtata gcttaggacg tcctttataa acaccgttcg cttttgcaat
    43381 cttaatgcct tgtgcctgac gtcgtttaga ttcggtacgt tcttgttcag cgatcatggc
    43441 caaaatttga atgatcaaat ctttaatgaa tttatccagg agaggattgc caattgcttc
    43501 ggccatgatc ggtagacttg tgatgatcag ctgcacattt ttcttcttca agtaattgac
    43561 tgaagcgatg atctcgtcgt aatttcttcc aagtcgatca atagcttcca caataaaaat
    43621 atcttgatct cggacgaaat ccagtgcttc ttgaaaaaca agtcgatgag taatcgttgc
    43681 gccagattgt ttctcaatga aaattctctc tgcaccgaat tttttcattt cttcaatctg
    43741 ccgttgttca ttttgatcga tggatgagac acgtatgtat gcaactctca aaatttttac
    43801 ctactttctg caactattta aaaaacaccc taatcataca cgagaaaacc agatttttca
    43861 atgtcgcggt cgtgtctagt tagggtatac cttatataga caaaaactaa tgatggagga
    43921 aatagtatgt gtcacttttc tttttaaatg gcaatatttt ttgtaaataa atgtcaatca
    43981 agaaaattta aacgattatg acattaaaaa tatattttat gaattttcta gtatataact
    44041 tgatttatct gttaagattt attaagaaaa cgttttctta tttttgattt cgaggtgata
    44101 gacaatgaaa aataagttta ttttatcttt aggtatttta actattggaa ttagtgtaac
    44161 tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg tagtagataa
    44221 taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc caactaacga
    44281 cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa
    44341 aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc
    44401 tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt
    44461 acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag
    44521 aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga
    44581 tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt
    44641 acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag
    44701 aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt
    44761 ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac
    44821 tttctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac
    44881 aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg
    44941 agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt
    45001 acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg
    45061 ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac
    45121 acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt
    45181 caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga
    45241 caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga
    45301 gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg
    45361 gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt
    45421 ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat
    45481 ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt
    45541 gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt
    45601 ttttgtaagt gtctactgat aacttattct caaatgttgt aaggatttta ttcatttgtt
    45661 aatatta
//
LOCUS       DACEDO010000014        43403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEDO010000014 DACEDO010000000
VERSION     DACEDO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.014"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..224
                     /locus_tag="IWO52_000847"
     CDS             <1..224
                     /locus_tag="IWO52_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355133.1"
                     /translation="KNKNRPPKKRWDDKKIDKAVLEMWKQVVTNLYQTIRKEKGVPLI
                     NGKEIWLEFIEEQGLIEFFNDSMAELEFE"
     gene            236..1384
                     /locus_tag="IWO52_000848"
     CDS             236..1384
                     /locus_tag="IWO52_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0355134.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(1813..3063)
                     /locus_tag="IWO52_000849"
     CDS             complement(1813..3063)
                     /locus_tag="IWO52_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0355135.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
     gene            3472..3948
                     /locus_tag="IWO52_000850"
                     /pseudo
     CDS             3472..3948
                     /locus_tag="IWO52_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
     gene            4058..5245
                     /locus_tag="IWO52_000851"
     CDS             4058..5245
                     /locus_tag="IWO52_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0355136.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            5414..5740
                     /locus_tag="IWO52_000852"
                     /pseudo
     CDS             5414..5740
                     /locus_tag="IWO52_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
     gene            5834..6136
                     /locus_tag="IWO52_000853"
     CDS             5834..6136
                     /locus_tag="IWO52_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355137.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(6172..6543)
                     /locus_tag="IWO52_000854"
     CDS             complement(6172..6543)
                     /locus_tag="IWO52_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355138.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(6545..6946)
                     /locus_tag="IWO52_000855"
     CDS             complement(6545..6946)
                     /locus_tag="IWO52_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355139.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKIK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(6960..7367)
                     /locus_tag="IWO52_000856"
     CDS             complement(6960..7367)
                     /locus_tag="IWO52_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355140.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            7711..8160
                     /locus_tag="IWO52_000857"
                     /pseudo
     CDS             7711..8160
                     /locus_tag="IWO52_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8290..9452)
                     /locus_tag="IWO52_000858"
     CDS             complement(join(8290..9176,9176..9452))
                     /locus_tag="IWO52_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0355141.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
     gene            complement(10129..10202)
                     /locus_tag="IWO52_000859"
     tRNA            complement(10129..10202)
                     /locus_tag="IWO52_000859"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10166..10168),aa:Ile,seq:cat)
     gene            complement(10392..11402)
                     /locus_tag="IWO52_000860"
     CDS             complement(10392..11402)
                     /locus_tag="IWO52_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0355142.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            complement(11399..11788)
                     /locus_tag="IWO52_000861"
     CDS             complement(11399..11788)
                     /locus_tag="IWO52_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0355143.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(11888..12028)
                     /locus_tag="IWO52_000862"
     CDS             complement(11888..12028)
                     /locus_tag="IWO52_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0355144.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(12021..12368)
                     /locus_tag="IWO52_000863"
     CDS             complement(12021..12368)
                     /locus_tag="IWO52_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355145.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            complement(12382..12876)
                     /locus_tag="IWO52_000864"
     CDS             complement(12382..12876)
                     /locus_tag="IWO52_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355146.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(12876..13325)
                     /locus_tag="IWO52_000865"
     CDS             complement(12876..13325)
                     /locus_tag="IWO52_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355147.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(13329..13949)
                     /locus_tag="IWO52_000866"
     CDS             complement(13329..13949)
                     /locus_tag="IWO52_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355148.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(13949..14857)
                     /locus_tag="IWO52_000867"
     CDS             complement(13949..14857)
                     /locus_tag="IWO52_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0355149.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(14870..17644)
                     /locus_tag="IWO52_000868"
     CDS             complement(14870..17644)
                     /locus_tag="IWO52_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0355150.1"
                     /translation="MDKDVYFFDDSQKLIKIVGEDKLFSVVQEKEITPSKDELINDKL
                     AVSMEFDDEIKESAYMAVRESESSFSMYKIIGIADPGSLLIFTGINFGPDELDAYIIN
                     DIRPANEFFQKTIQRVIDFTLGEWRVGHLDSTLPAVSMTFYYCSIREALKNLQTLGCE
                     IVFRCNLSGEGITDKWIEVYKQIGEYSNERYEYGDKALTIEKEVDRSNIYTSLIGRGR
                     GEEVGDGYGRRIEFDQVYWSKSKGDPLNKPTGQIYLEIPEMTEKYGIPTKNGKRRKRE
                     KVIIFEDCEDPVELIQLTYQELVNCSRPLVQFKATIFGADSLGNIIRIHRDDRGYHYE
                     TRIFSVKIDRLTGKVETGLGDNLNTSSTRQASNTQTAIQTLDEKKMTFYESTEVSKWQ
                     SDIIRGAKGGSIIMMNPWDTGKGESRQPYQMVWMNGDSIDTSNHFLVANSEGIGFIDG
                     KFNESNFKTAWTIDGNFNANYIQSGRIRADIFETSFNAVGDQLKLVKGALQIVNSNKK
                     IMELTKKGMEFWNTKESIGTIGTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILI
                     SPKVGKGLVLLGNGKAIYFGDLDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEK
                     RAWKIWTMLKARGYSEYAAAGILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPL
                     VGSPTWNGREYVQRLMNTAGIQEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKS
                     INDAKSAAYAFEMNFERPASAHPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTV
                     TSECGWRTSPINGGQEFHNGIDLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIK
                     HNNGKWTGYAHLSRIDVSVGQKVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVD
                     FENPRNYIQF"
     gene            complement(17644..18426)
                     /locus_tag="IWO52_000869"
     CDS             complement(17644..18426)
                     /locus_tag="IWO52_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0355151.1"
                     /translation="MEWHNPMYEFRDTVKNDSQKTWIPTSALNYDGKFIENYIEGYQT
                     LYVEGREMVSLEIESEAVSIGVRISSQRLPERILTIHFKLEEKNPIEFQRSFNKLMRL
                     LYRDKDVEIHFNDELDMYYYGRYQTCDNIPGNVHSVISSFSIICSDPRKYTRIFETNG
                     IVSEYLPYEVAPISISLKANNDGSLRITNGRQNISVTNSMIKKGDFIEMDIAEGKVFV
                     NGVNKTRILDLTSSFKNFMVRTGDLVKCDNGTPLIRYRGVWL"
     gene            complement(18444..21266)
                     /locus_tag="IWO52_000870"
     CDS             complement(18444..21266)
                     /locus_tag="IWO52_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0355152.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEEKSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEEQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQRLVVA
                     IGAIAIAIGPVLYALGMLVKAFQTMKVGLGVLGNGISLFKKLGSAIGFLTSPVGLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTIQEAPGKAADWIKNKWTETKEFFSSIWDGIKEAASSAWEGIVNILTP
                     YVIAIKNVFQPMIDFFTNLWSQIGSIAGSAWEIIKTAVMGPILLLIDLITGNFNQLKE
                     DASMLWTTLTTNIQNIITTFVDIVVGYYTALKDTVINIWNVLTSTIKDVWNSFTTWIK
                     ETTNNIVNSIKQGWNNLKQGTIDLFNNMIQGAKDLWNSFKAWFINLVIGTKDNIIQGW
                     ENLKQGTIDTFNNLVNGAQEVWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDS
                     FLEGLQNAWKSVQDFVGGIGDWIREHKGPIRYDRKLLIPAGQAIMNGLNAGLTNGFAS
                     VQSNVGNMANMIADSFTRTPDIDLSANLKNANRNFTTQIEHSVNYGKNKRPAVFNIRL
                     GNQVFEAFVEDISNIQGKEADINLLF"
     gene            complement(21283..21570)
                     /locus_tag="IWO52_000871"
     CDS             complement(21283..21570)
                     /locus_tag="IWO52_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355153.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(21624..21992)
                     /locus_tag="IWO52_000872"
     CDS             complement(21624..21992)
                     /locus_tag="IWO52_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355154.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFIDGNMEFGMGLSAKVL
                     PELRSYNANTLSRVLEIANRTEEETITLDEMDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQAE"
     gene            complement(22038..22550)
                     /locus_tag="IWO52_000873"
     CDS             complement(22038..22550)
                     /locus_tag="IWO52_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAQ0355155.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDD
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(22561..22950)
                     /locus_tag="IWO52_000874"
     CDS             complement(22561..22950)
                     /locus_tag="IWO52_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAQ0355156.1"
                     /translation="MIKTRDQSLFDEMFARSLALGYQTYDYKPLDDVGYPFVELESSQ
                     TVHNPNKTDIKGTVNLTISVWGLQKKRKQVSDMASALFNQALQIQATEGYYWDLNYQA
                     SGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(22947..23318)
                     /locus_tag="IWO52_000875"
     CDS             complement(22947..23318)
                     /locus_tag="IWO52_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0355157.1"
                     /translation="MKVSLGYKGVDELLKHLEEAVTLRDVQMVVKTNGAELTKRMQEK
                     ARFNGHWEGDVFVHPTGFTRRSIRMWLLEGGFVAQVGPQSDYSPYLEYGTRFMSAQPF
                     VGPAFNVQKAIFMKDMQRLFK"
     gene            complement(23315..23629)
                     /locus_tag="IWO52_000876"
     CDS             complement(23315..23629)
                     /locus_tag="IWO52_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355158.1"
                     /translation="MRFLDEVIFEKDGEGNRYDPELGEWIEEAPITVTTNANVTDLGT
                     DRSVAIFGDIRQGAKVIRTMPLFVVPKYDRIIFNGKTYKDVAQRSLLSTNSIIVQEVI
                     AK"
     gene            complement(23626..23955)
                     /locus_tag="IWO52_000877"
     CDS             complement(23626..23955)
                     /locus_tag="IWO52_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAQ0355159.1"
                     /translation="MVIKDDVKKLLSGSTDDKLEVIERRTRERLASLLGVSVTPESLE
                     YIVFDVTNKRFNRIGQEGMTSYAQEGLSMAFPDSDFSEYASEIDDFKRKDDEELYKPR
                     KGSVRFL"
     gene            24005..24292
                     /locus_tag="IWO52_000878"
     CDS             24005..24292
                     /locus_tag="IWO52_000878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355160.1"
                     /translation="MCDPSSVFIVVVYLPAGTVSVQVILPLEETVNPLVTGAIEYVTV
                     LFVAFCGATVAVNCLLPAVPATALEVLGVTVTPITGIVTPPPVQFYRTQHL"
     gene            complement(24249..25136)
                     /locus_tag="IWO52_000879"
     CDS             complement(24249..25136)
                     /locus_tag="IWO52_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0355161.1"
                     /translation="MAQTWNPDNVTVYETKEGKIPDKYNTLIINDIMENSKVMQLAKY
                     EEMTDKEKKFEYFAEGPGAYWVGEGEKIQTSKPKWLQATMVAKKLGVIIPVSREYLHY
                     KMSDFFTVMQPKIAEAFYKKFDAAALLNTDNPFPQSLEESVVAAANVIKGPLTYDNIL
                     ALEDALGKNEFEPNAFISNRKNRTELRSAAQTVGTNVEFIYDRSANTIDGLPVVDLKA
                     LGKGELYAGDFNYMFYGIPFNISFKISEDAQLSTLKNEDGTPVNLFEQELVALRATMD
                     VGFMIVKDAAFGKIEPAGE"
     gene            complement(25151..25753)
                     /locus_tag="IWO52_000880"
     CDS             complement(25151..25753)
                     /locus_tag="IWO52_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0355162.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPTEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELEAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            complement(26056..26283)
                     /locus_tag="IWO52_000881"
     CDS             complement(26056..26283)
                     /locus_tag="IWO52_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355163.1"
                     /translation="MHLYQNDAQFKEDYDAVKQHLIDVLEPIVKEVYETARKVIEPVV
                     QSITELVQSNPEYFKALQNNMNQSVFQSASM"
     gene            complement(26361..27317)
                     /locus_tag="IWO52_000882"
     CDS             complement(26361..27317)
                     /locus_tag="IWO52_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0355164.1"
                     /translation="MKSQDYFIKREKAWQEQQIKDDKKRMNEIKKRLQYAQDAIQKEI
                     DAQWNSFSNGQKITRSEAMKRANEMDVKAFARKAKKYVKEKDFSPTANKELKLYNLTM
                     RVNRLELLKANIGLELIATFNELDKYFSNELTKAGLDELKRQAGILEMAIAKSGYAKL
                     VEQVINSSFRADGFATFSERLWMYQAELKADLDKLLVRSVTMGRNPKQLAPELSKFLT
                     EEGRENTKFNTQRLMVTETTRVQSGIQRESYLDANIEKYGWVTEPNACRLCKSIAADG
                     PYLVSEMEVGKNMMPVHPFDRCSTYPIVDRDAFEKSLKERRL"
     gene            complement(27322..27585)
                     /locus_tag="IWO52_000883"
     CDS             complement(27322..27585)
                     /locus_tag="IWO52_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355165.1"
                     /translation="MKARKKPVTVEIVQLKMLSARSYRKCKEFVGEAWIDHNNMPNGL
                     PGIETLEGTMEISDGDYIIKGVHGEFYPCKPDIFIETYEIIEE"
     gene            complement(27572..29068)
                     /locus_tag="IWO52_000884"
     CDS             complement(27572..29068)
                     /locus_tag="IWO52_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0355166.1"
                     /translation="MDKVNEFEYGTDIHYSNDVNTNYVKFSVESNLHYRFSSAEDLLN
                     DSNTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKDRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNAETSADRGVFDSDKETDTEVEEDEST"
     gene            complement(29081..30325)
                     /locus_tag="IWO52_000885"
     CDS             complement(29081..30325)
                     /locus_tag="IWO52_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAQ0355167.1"
                     /translation="MGLVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDIAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRVKRMF"
     gene            complement(30312..30845)
                     /locus_tag="IWO52_000886"
     CDS             complement(30312..30845)
                     /locus_tag="IWO52_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAQ0355168.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            complement(30872..31096)
                     /locus_tag="IWO52_000887"
     CDS             complement(30872..31096)
                     /locus_tag="IWO52_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355169.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            complement(31103..31315)
                     /locus_tag="IWO52_000888"
     CDS             complement(31103..31315)
                     /locus_tag="IWO52_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355170.1"
                     /translation="MNENQLRELFKTNEANQTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNEGQAMEFMMRTFSANNQQRE"
     gene            31798..31977
                     /locus_tag="IWO52_000889"
     CDS             31798..31977
                     /locus_tag="IWO52_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAQ0355171.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            complement(31970..32383)
                     /locus_tag="IWO52_000890"
     CDS             complement(31970..32383)
                     /locus_tag="IWO52_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355172.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(32644..33060)
                     /locus_tag="IWO52_000891"
     CDS             complement(32644..33060)
                     /locus_tag="IWO52_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0355173.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHVNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            complement(33136..33432)
                     /locus_tag="IWO52_000892"
     CDS             complement(33136..33432)
                     /locus_tag="IWO52_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355174.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDIKLINDK"
     gene            complement(33456..33761)
                     /locus_tag="IWO52_000893"
     CDS             complement(33456..33761)
                     /locus_tag="IWO52_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355175.1"
                     /translation="MNVQVYLKRDLARFPRSWDEYSCPFEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(33777..33974)
                     /locus_tag="IWO52_000894"
     CDS             complement(33777..33974)
                     /locus_tag="IWO52_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355176.1"
                     /translation="MNKRQKNKQFKKQLIKNGYPDKKVKLYCSECGNRLSFKNEYHKR
                     HLLCNEFCFMHSVGLGWNDFL"
     gene            complement(33971..34270)
                     /locus_tag="IWO52_000895"
     CDS             complement(33971..34270)
                     /locus_tag="IWO52_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355177.1"
                     /translation="MSDYLTAEQYEYARKTGDWESGKVLTHGQALNDNQKIVLEWLEE
                     YYAKSQWPFPFYVLKHAANDMPTNIFGFYDDFTMKEEFEVLAAFAQWGLKQEEAE"
     gene            complement(34267..34851)
                     /locus_tag="IWO52_000896"
     CDS             complement(34267..34851)
                     /locus_tag="IWO52_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0355178.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDNLTKAEKIAYLIKSKDGDSYYFCDWFVRDGILTQEQG
                     EELLAWATRQSYETLLSLYNGYEVEKEPLYEVIIGDLYLIKKFNNRNDFCFDISRSLC
                     TWEKSAYQLTEAEIKEIDERYWPFAVPVEEVVER"
     gene            complement(34848..35261)
                     /locus_tag="IWO52_000897"
     CDS             complement(34848..35261)
                     /locus_tag="IWO52_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355179.1"
                     /translation="MIPKFRAWVKPDILSNHLNGVIAEALPNFIYGNCLVLREDLEED
                     KQFEEIIDFLDIELMQSTGMKDKNGVDIFEGDIVKTLDADLKVTFSTIKFEEGAFCVD
                     YKDLGTEFDFLYFVDSPLEVIGNIYENSELLEEQR"
     gene            complement(35258..35503)
                     /locus_tag="IWO52_000898"
     CDS             complement(35258..35503)
                     /locus_tag="IWO52_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355180.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(35463..35819)
                     /locus_tag="IWO52_000899"
     CDS             complement(35463..35819)
                     /locus_tag="IWO52_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0355181.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(35819..36124)
                     /locus_tag="IWO52_000900"
     CDS             complement(35819..36124)
                     /locus_tag="IWO52_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355182.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(36121..36282)
                     /locus_tag="IWO52_000901"
     CDS             complement(36121..36282)
                     /locus_tag="IWO52_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0355183.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(36297..36656)
                     /locus_tag="IWO52_000902"
     CDS             complement(36297..36656)
                     /locus_tag="IWO52_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0355184.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(36653..37504)
                     /locus_tag="IWO52_000903"
     CDS             complement(36653..37504)
                     /locus_tag="IWO52_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0355185.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(37521..38351)
                     /locus_tag="IWO52_000904"
     CDS             complement(37521..38351)
                     /locus_tag="IWO52_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0355186.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(38354..39040)
                     /locus_tag="IWO52_000905"
     CDS             complement(38354..39040)
                     /locus_tag="IWO52_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355187.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(39046..39717)
                     /locus_tag="IWO52_000906"
     CDS             complement(39046..39717)
                     /locus_tag="IWO52_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0355188.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGDFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(39710..40051)
                     /locus_tag="IWO52_000907"
     CDS             complement(39710..40051)
                     /locus_tag="IWO52_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355189.1"
                     /translation="MENELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(40377..40565)
                     /locus_tag="IWO52_000908"
     CDS             complement(40377..40565)
                     /locus_tag="IWO52_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355190.1"
                     /translation="MSYTLQQEHQILRLIKQRRKQLQDDREALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(40586..40870)
                     /locus_tag="IWO52_000909"
     CDS             complement(40586..40870)
                     /locus_tag="IWO52_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355191.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVRKVLGIGDDKLTHWINLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(40848..41060)
                     /locus_tag="IWO52_000910"
     CDS             complement(40848..41060)
                     /locus_tag="IWO52_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0355192.1"
                     /translation="MSQDFILKVRVALATHNKSQAWLAEKLDISTAYMSDIMNGRRKP
                     DKQIKPIEAVLAELEKEKKHANNNSR"
     gene            complement(41063..41839)
                     /locus_tag="IWO52_000911"
     CDS             complement(41063..41839)
                     /locus_tag="IWO52_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0355193.1"
                     /translation="MNTPQIFNFGQQEVRTVLLNDEPYFVGKDVAEILGYERADNAVR
                     NHVDEEDRLMHRISASGQNRNMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEER"
     gene            complement(41965..42186)
                     /locus_tag="IWO52_000912"
     CDS             complement(41965..42186)
                     /locus_tag="IWO52_000912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355194.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPREHQKNSFATG
                     EKKTQKRSEKVLRIIQFLLEKIRHFGNLF"
     gene            42484..42831
                     /locus_tag="IWO52_000913"
     CDS             42484..42831
                     /locus_tag="IWO52_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0355195.1"
                     /translation="MTTFERVKMLADKRKISIVELEEKLNFSKNSLYAWKKSKPSIDK
                     LNAVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREIL
                     SAMIEAYLEKKDN"
     gene            42867..43262
                     /locus_tag="IWO52_000914"
     CDS             42867..43262
                     /locus_tag="IWO52_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0355196.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
BASE COUNT        12558 a         8866 c         6964 g        15015 t
ORIGIN      
        1 aaaaaaacaa aaatagaccc ccaaaaaaac gctgggatga taagaaaata gataaagctg
       61 tattagaaat gtggaaacaa gtcgtgacta acttgtatca aacaattcgt aaagaaaaag
      121 gagttccatt aattaacggg aaggaaatat ggcttgaatt tatagaggaa caaggactga
      181 tcgaattttt caatgatagc atggcagaat tagaatttga ataggggtaa tattgatggc
      241 aatgataaaa caatataaaa agaaaaatgg cgaaaaagca tggtacttta aaacttatct
      301 cggtattgat ccgctaactg gaaagaaaaa atatactact aaacgaggat ttagaacaca
      361 aaaagaagca aaaacagcac tttctaggtt agaactagaa ttacaaaaaa caggaatgcc
      421 cacaagtaca aatactactt tcaaagaagc agcagaatta tggctagaaa gctacaaaaa
      481 aactgtaaaa gaaagttcat attcaaggac taaaataatc tttaataaac atatatatcc
      541 caaatttgga aatattaagc tttctaaaat taatacggca tattgtcaaa aggtagtaaa
      601 tgattggagt gaaaaaggaa cttcaaagca gtaccctctt ttcataaact atatgaataa
      661 agtttttaag tatgctataa atattggttt aacatctgat aatccaacat taaatttact
      721 tattccaaag ccacaaatta aaacagaaaa gaaattaaaa ttatatacaa aagaacagtt
      781 ggaattattt ctaaatgaag tatctcaaga acagaatcca tattttaaaa acagagacta
      841 tacgctcttt agactattag cattcagcgg atgtagaatc ggcgaaatat tagcactcac
      901 ttgggacaat attaatttta aaacaaatga aatggccatt aaaaaaactg tagctcgttc
      961 agataaatat tatatatctg aaactcctaa aacaaaaaaa tcaaatcgaa taatttattt
     1021 agatgaaaaa actataaagc aactaaaatt ttggaagctc gaacaaagaa agtacttatt
     1081 tcaattagga tttactaaag ctaattattt gtttaccaat gacgaaaata atttcacaat
     1141 taatcagtca gtggcagaaa gatacaatat atatcgtgag cgtgccggct taccttatat
     1201 cggtctgcat ggttttagac atacacatgc atcaatgcta tatgaggcag gcgcagatca
     1261 caaagaagtc caagaaagaa tgggccacgc aaatataaaa actactatgg acacatatac
     1321 acacattact aacagcaaaa aagaagaaac aacacaaaaa ctaacaaatt atattaactt
     1381 ctaagaaagt atggtcaaaa gtatggtcaa aaaaatccct tacttagcaa aaaaagctcc
     1441 atcccttgcc acacaagaga tagagcagtt tatttattaa gcttcttgag ctactggata
     1501 aacagacact tcaattatac tgtttcctga taagctctca atgcttctga acccatgata
     1561 ttagtgcttt tcttctatta ttaaatatct tgtaattttc tattctttct aaaaagtatg
     1621 gtcaaagtat ggtcactatt gatagaatat taatagtagt agaaagctga tatctagtct
     1681 aatggtgata agtcaagatg aaatttaaga ttttctttat tgagtaatat gttttgaagc
     1741 gcacgaaact agacccgatc aaaatcaaat ttttttcggg ttgattcaag gatttgcgtc
     1801 aataacggat tgttatttct ctatcacact ccttggtggc gtatagagtt ggtgtttacg
     1861 tagtagcgta tccaccagac gcgtaaattt tcttgcggtt aggacgagtg cacgtttgtg
     1921 ttgatgcttt ggaacctcct tatatttact ttgataaaaa gctttgtatt caggaagata
     1981 attttttaca gagttggcag cttcaactaa gtagtatcta aaatatcgat ttccttgttt
     2041 tgtcagaggt gtattttgcg actgatagtt tccagattgg ttcactttcc aacttaatcc
     2101 agcgtatttt gccaattttg tctgatcttc aaatctttcg atttgaccaa tttcagcgat
     2161 taaaccggca gcatagactt taccaacgcc tggaatactg gttaagcatt gatattctgg
     2221 aatgacaaca attacttgtt cgatggcttt atctaattct ttgatatttt tttcaagtgc
     2281 tcgtatttct cgtacgagaa cacttaaaac aacattaatt gattcttgtg caagagcacc
     2341 taggcggtaa ctcatggtaa ttgcacgttg aattgcttta gcgatttttt ctggttgctt
     2401 gaaacggcct tttccttttt cttgaagtaa atcacagaaa gcttctaaag gaagctcagc
     2461 aagttcatcc aatgtgaaat cttccgtcat gagtgaaatg agggttgcac taaaaagact
     2521 cgtcgattcg tctcgcattt ctcgagcaag cgtattacat ttgtacgtta gattttcaag
     2581 aaaatgttgc tttgtacgaa tcaattgctt aataagttga tagcgagtac gtgttaaacg
     2641 ctgtaaggcc atgtatttct cttctttgat aggggaagtt gtgaatcgtt gaatacgcaa
     2701 gaaatcagcg attcttaatg catcgtttcg atcagtttta ttctcatcaa acatgcgact
     2761 aaattgtttc acccgaaaag gattttcaat cgttacaagt gtgttgagct tctttagttt
     2821 ctcatcttca tgaaaaaaca tggaaggatg gaagctgtac atggacgttg actccatgcc
     2881 aatcacaatt tgatcaaagt ggtagctgtc attgaattca agaattgttt ctcgtatgaa
     2941 tgaagcacct tcgatgtcat tggcaacaga gatttcagat aagatagaca attgattgtc
     3001 gtctgttaaa aaacaaacat ctagtttttc agagctaacg tcaataccga caaataattt
     3061 catggaagaa ggacctcctt tcgatataaa ttttggaaaa tgcttcgata ctgtgggatt
     3121 tcccagaaac tcactgtata gccacaacct cgcctaatag aatacggtga taaaagagct
     3181 agaagctgcc ttccaaacta ctatttgaaa gtctagtcca ttatcgaaac agctaatgag
     3241 ttggtagtga taacagtggt tcgggtaaca gactaaacag ggtagacaaa cgctacagga
     3301 ggaaatagca aattcccgtt ggatcctttc catacatcca ttgtactctg agaaatcaca
     3361 caatatcgaa acaaattccg ttaggaatgt gttattcaat aaattttata atctagaaac
     3421 attttgaata aaaactatta gacaaatgat attttacgag gaggaactaa tatgagtaaa
     3481 caatgtgcaa agtgcggagg taaaattggc ttaacttcat ataaaatcaa agataaacaa
     3541 attgtttgcg gcaattgtat gaaaaaagct ggttacggta tgactactcc ttataaaata
     3601 ataagatctt tgaatttaga agatttagat aagggtccat cagaaaaaag aaaattaaac
     3661 atatctgatt atactacaca aaacaatcat gatataggta atgatagaat gagaactact
     3721 gaagagatgg tagaattttg tcttaaatac ggctatggta aaggaatgac taaaaaatgg
     3781 acaactcatc attttaactt aatatcagat caacttaaca gtgacgaata cgtaatcttc
     3841 tgctttgtag gattgcataa ctatatctcg tcaactaagc atgacaataa ttatgcatac
     3901 gcattaacta ataaacgttt aattgtagct caacaaaaaa tgataggcag attacgtcca
     3961 tataattgtg taaaagtgaa gcatcaaaaa agccattgat ctcagttagg agtacaatgt
     4021 tttttgcaga aaacatactc gaataggact gatatcaatg actcaagtac attttacttt
     4081 tgaaagcgaa gaaattcaag caattataaa cgaaagcggt gcgaatgata ccgcaaaaac
     4141 acttatgact atcatgttta atcagctgat ggaagaacaa cgaaatcaat atattcaggc
     4201 aaatgcctat gaacgttcag aagaacggca atcacaaaga aatggttact acgatcgttc
     4261 gtttacaact cgaattggta cgttagaact tcatgttcca cgaaccagag acggtaagtt
     4321 tagtcctact atctttgaaa gatatcagcg aagcgaaaaa gctttgatcg ctgccatgat
     4381 tgaaatggtc atttctggtg tctccactcg caaagtaact aaaacagttg aactgctaac
     4441 ggatggtgcg actgtttcta aatcattcgt ttcaaatctg atgaaacagt tggatccatt
     4501 tgtttttgag tggagaaacc ggagcctaga aggctcggaa tatccatttt tcatgtgtga
     4561 tgccctgtat atgaaagtaa gggaaaatca tcgtattgtc tcaaaaggtg tatatattgg
     4621 tatcggcatt gattctgatg gacgacgtac gattcttggt tttgatgttc aagacggcga
     4681 atcggaagat aattgggata ccgtttttca atcgttcgtt caacgaggtt tgttcggtgt
     4741 aaaactggtc atttctgatg cacacaaagg gttagttaag gctgttcgca agaacttttt
     4801 aggtgcgagc tggcaaagat gccaagccca ctttttgaga aatatttttg ataagctgcc
     4861 aaagaaagtt tcttctgatg taaaagacga gttgaagagt atctttaaag cctctgaact
     4921 tgaattgaca cgtgaaagaa aagagcactt cttggaaaaa tatggttgtg attcaaaatt
     4981 aagtgctgct tgcgacattt tagaaaacgg ctttgaagat gctattcaaa ttttgtcttt
     5041 tcctgaaaat atccgccgaa ggattcgtac aaccaatgtg ttggaacggt tgaacgaaga
     5101 gattcgccgg agagaacgag tgattcggat ttttccaaat atcaattcca tcactcggat
     5161 aattggaaca cttttgatgg aaaaagacac tgagtggctg gcttctccac gaaaatattt
     5221 agaatttaat tcgaataaca tttagcacca tgaacctgtg cgagtctctt ctttctgagg
     5281 agagacatca agagtcgctg cgctactgct cctgacctct ctcctcagaa agaaaggcat
     5341 ggcagttagg ttcacaacgc aaaacattgt gcttttgatg aggttgaact tttacacaag
     5401 attgtggact tgactgatag gcaataatgt acaaagtatt atattagaca atttaaataa
     5461 tattagtaaa aaaagagaaa tactgttggg tacattaact attgatactc ttggtaaagt
     5521 attgaatgtt gcagttgata aagatactgt tgataggata agtgattctt taaacgaaat
     5581 tatttataat ttaaaaagaa gaaatatttc tgctataaat tccaatacat caacaatttc
     5641 ctcagcttca gaaataagaa aatataaaga actattggat gacggaatca ttacagaaga
     5701 agaatttaat aaaaagaaac aagaattatt agatttataa tatctatcaa aaaagatgag
     5761 catttaattt agctcatctt tttctattta caatacaaac atttgttcgt atactctttt
     5821 tgaggtgaac attatgttga tggaaggaaa aacacaatta tggtttaaat ttgatccttc
     5881 gaatagattt atcaaagatt tttataaagt atggaattca gaagttttct ttttagcaat
     5941 cgaagatagc ttattaatca atctctacta ttctaataag aactatttca aaattcctgc
     6001 tgcgaaaact agaatgaaga agggtgtata ctttttgttt gatatcgtga ctgacgtgcc
     6061 ggacgcacgt agcgatcatc ggcgttatga ctatataaag tatactttcg ttgatccaga
     6121 aagatacaaa gattaaataa aaaaataaca aacaaactcg cttgaaattc attaggtgag
     6181 tttgtttatt attttttctg cttctcgatt catcagcgcc atatctgttt tcgtaatggt
     6241 ttcattagga atatagtcag cacattttct tttctctaac attctatacc cactattatg
     6301 tattcctaag gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg tttggtggct
     6361 tccttttccg ctacttccat tataaataat atcataattt tctttcaaat actcatcaga
     6421 ttttttaaaa gcactgtagt atgctcttga aacagcagtt cttctataag attctttaga
     6481 attatattta tcatctgatt caatttgttt ggaaatagat aaatattcat taaagtcaaa
     6541 cattttattc ccccaaagtt gctactacag atttccccat atatttatag tcagataata
     6601 cattatcgag agtatccaaa acatcgtaat ctgaggaaaa caattcagat gttgttttga
     6661 taacaaagaa atacaagttt aaatcttcat catattttat attgatttca gcttttcttt
     6721 ttaaaaccgt atttatcaca tcaaaaattt tgccatcatt acttgaaatt gtgttaatct
     6781 cattttttgt agtaattaca agttgattat catttttaat tttcggagta gaacttgcat
     6841 ccacaacaac tggagatgct agaattaccg atgcgcaagc aaaaatcgca gcaaaattta
     6901 ttaagctacc actccgatgt gtatttgatg gtactatatc aagcatccgt atcgtctcct
     6961 taattttttt ccactggttt gttgaccttg atatcaaaga tttgccttcc aatctcagtt
     7021 ccattaaatt taacaacagc ataatgctct cctgcttctt caaccaagac atttctggcg
     7081 tttatattaa agtttatatc gttaacttga ggaggcaagc ttaccgatac ttcacccgta
     7141 gaaaaaattt gtttttcatt cttgtttaaa acttcaattt ctactatttt tatcttttct
     7201 gtatcctcta tctcagacac cacagctgtt acagaaaatg ataaagcagt aggaattaaa
     7261 ggcgcattaa tagaaagagc tggattaatt actttattaa taacattagt gccaaattgc
     7321 tgactttcta catcttctga aattattaat tgtactctat aagacattga tttctcctaa
     7381 tttttacatt tatttaataa atttatgttt cacttttgct agtcaattta gtttaacaat
     7441 agtaacaaag ctttgtcaac ggaaatatta gctaattttg taagcatatt tataagtata
     7501 tttgtaagca tatctgacat ttaatcagta gtaaaagtgc ctctatataa ggaacaaact
     7561 gtttttttac tatctaaatt cgtatacagt tttactaaca aattagctaa caatattagc
     7621 aactatatca acaactatat gtacttcccc tcaaaaatga tgtgcacccc aaaagttaga
     7681 ctagaaatct aattaaaagg ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa
     7741 aaagtagttt cagactattt aaataacgaa ggcggctaca aatatcttgc acataaatat
     7801 caaataaatc gtacactggt tagacattgg gtaaggattt ataactatca tggttgggaa
     7861 ggcttggttg gaggtggcaa aagctacact acaaaattta aacttgatgt tatagaatat
     7921 atggaaacaa atggtctttc tatccaagaa actgctaaaa aatttaatat cggttcaaat
     7981 agaactctaa gtaaatggat agagcaatat gaagaaggcg gtgcttcttc acttgagagc
     8041 caaaaaaggg gcagaaaaat tagtatgaat tccaagctaa acattcctaa aaaacttaaa
     8101 gatgagtctc ttgaagaaga agttattcgt ttaagagcag agaacgcata tttaaaaaag
     8161 tggaatggca acactttttt tgacaaaaat tataaggtgt tgtgaatgct gcggtgttat
     8221 ggctacgatt gcccttgaca atgagcatcc tcggatctga ttttctccgg cagaagcaca
     8281 atcattcaat tacgctgcct ctccagaggt atcagatccg cgctcattat caaggattcg
     8341 cttcgccaat ctctgttgtc gaatttggat cggtgtttca tacgccaacg tgccatgtaa
     8401 gcgtagatag ttccaccaat gcacataatc aaacaattct agacgtagct gtgccagtgt
     8461 ctcaaattgg tattgatgca cgaattctac tttgacagat ttataggttg attccacaac
     8521 ggcattatca tacggacaac ccttcctact caatgaacga gtaattccaa aaccattcag
     8581 aatctcatga atggtttggt tatcaaattc ctttccccgg tctgtatgaa aaagcttgac
     8641 gtctgttaaa gaatacggta tccgtccaaa ggcttctttt accaatgagg catctttctt
     8701 ctcaccacag gaataaccaa taatttctcg attaaacaaa tcaagtatta agcagatata
     8761 atgccacttt ttccccacgc gaacataagt aagatccgta acgatggctt ccaatggctg
     8821 ttcttgtgta aaggtccgat ctaatacatt cgctgttttc gcttcattac aagctgttcg
     8881 ttgcccttta aaatgagcga tcgtataggt agatgtcaat ccgcggcgtt tcatgattcg
     8941 accgattcgg cgccgactga gttgaagccc acgctttgct aaacacttct tcaattttcg
     9001 ggtaccgtaa gcctttcggt ttcgaataaa ctcttcctga acgatctctt ctaagtcgga
     9061 ctcattttcg atcggtttcg cttgataata gtaggtttga cgagaaatat ttagaatttt
     9121 gcacatcgct gatatggaat atttatgctt gttggcatca attacttgtc ttttcgtccg
     9181 aatatcagcg ccgcttgctt taaaatatca ttctccattt cgagttgctt atttttcttt
     9241 cttagtgcga tcaattctgc ttgttctggt gttaagttgt ctctttcttt gaatgagccc
     9301 gttgattgtg cttgcttcat ccatttatcg aaagaagaag gcgtaagctc atactctcga
     9361 ataatttctg cgcgaggctt accagcgaga tagagatcga cgatttgttg tttgaattct
     9421 tttgaatagg ttcttcgttg acgtcttgac atgaaaaatc ctccagtgtg ttttttatta
     9481 ttctacacac cttatttttt ctgtctagtc tagtgtagcc gattcatgac tgacgtgccg
     9541 gacgcacgta gcgatcatcg gcgttatgac tatataaagt atactttcgt tgatccagaa
     9601 aggtataaag attaaagtag gctacctaaa aaggtagccc ggaacgaatt ttatcaccat
     9661 acttgtgaaa ggagatattt tttaagttag tattaagatt gtgtaatatg atgatatcta
     9721 tattttatag tatcagtgct ataaaatcaa aaataagtca ctaattaact accactccaa
     9781 ttgtaagcct tttttctcac tttttttcaa aaatatggta tgctttttaa tggcttcaaa
     9841 tataaaagag tttaaagcgt aacacactta tggggaagtg gtttggggtg cgctttaaac
     9901 tcttctttat tattatctca caatttaacc caaatgtctt tctatttaaa aatcaaagta
     9961 aaacttttca aatatacaga agtataacta tgtgaaacat cctttcatta atccataaaa
    10021 ggatacataa aaaagccact catttgagtg gcaatgaaga aaagctttag cttgtataat
    10081 actcttcaaa aaaattctaa cacagaacga ttcaaatggc tacgttaatg taccctgtag
    10141 gactcgaacc tacgaccgga cggttatgag ccgtctgctc tgaccaactg agctaagggt
    10201 actggttgtt gccacataaa gccataaaca atcaaccagt agaatgtgtg gcaacaaacc
    10261 tgttatcgca tagcttggag tgtgactatt tatgggtgat agtgaagata tgcgataaca
    10321 tcactatttt atcgaataat ttttatagtt gtcaatatag ttatgtactg ctcctcaacg
    10381 aggagctatt tttatcgttt aggaatattt aaataccaac gtttgtcatg gaaatcttgc
    10441 gcaccgcctt tagtgtttcc ctctggatca ttcgttgccc gcatcattac atagactttc
    10501 ttactaggaa aattacgcat attgaaagat acatgataac caacatttcc agaagtatta
    10561 taagcttgat ttacatccgg tctataaatt ccatcagctc ttactcgagc taattctttc
    10621 ccagtattgt agtccataat gaaaatatac tcgtatttat agttagcgat gtgccatcca
    10681 gcgacgtgta agttcgcatt ttcgatttct ccaaactgat caatgtgaga atggttcgtt
    10741 ccatctgata aagttggatt tgctgcacct gctcgggttg gatcaatgac aggcttgttc
    10801 tcagaagtcg ttgggttttc gtctgtaaat ccatgagcta aatcgtaagc cagtttttcc
    10861 ttacttacac ccatttctga taagtatcca tacggatcag tgtgatcgcc ccaaatgttt
    10921 tgagttaccc ataaatgtga tttgattcct ggttgattat acggtgtgtc taatgtaagt
    10981 ggaataccgt atttattagc ggaatctctc gctaattcaa catatgcctt gtagttcttt
    11041 tcaaacgtta ctttatcgtg cgtatgttgc agttcgattt gcacaggact atttgcatta
    11101 gcatatgaac cagcgccata ttgtacataa ccaggttgtc caacttggta aacgatccca
    11161 ccatcgccaa caatatacgc tgtataggca ttgtaccaag aacgtttcat gtactgcgct
    11221 tcatttcgtc ctgtcgcagt ttcgtttgct gtttcatgca ataggatgta ttggttattt
    11281 gctacttgag aactaccttc gtttgcgccc aaattgaatt cattgttgat cgagtaagca
    11341 aaggcgttta ttggcaataa aaaaagagct ataatagctc ctacaaagat tttctttttc
    11401 atttggttcc tcctcggtct ttattttcag tatcatttcg tatttgttgg aatacctctt
    11461 ttaacttatc tggcaaagga aggaataccg aaatgttttc taaaaaacta atcccttcat
    11521 ttgcaacata gaacataata actatttctc ttagaggtat agaattccca acgattttct
    11581 gtacgtcaac cgcaacggca atcacaacga agattaagac tttcttcaac aatccttcga
    11641 aacctatctc actacttatt tctttcaatt tccatgcctt agcaagtcca gttaagaagt
    11701 ccacacccac aagaaaaatt aaaacatgta gtatattgtc catccctccc aataaaccaa
    11761 caattgtccc gcctatcacc cctgtcatta ctgagagtgt atttaaatat ttttccaata
    11821 tctatttctc ctttatgcaa agaagcgcac tcgtttgagt acgcttttta tttaactttg
    11881 tgcttaatta aatttcattt ccagtaatct gcttatattg gtcttttgtt aaacagttca
    11941 tgtttacata gtcaagtaaa tcttggtccg tataacatcc ccaatcataa aattgtttta
    12001 tattttcaaa gcccggaaaa ctattcatta cttttccctc cattcttaat acttgcgact
    12061 tccatcgtca attgagctaa catttgttgc attttttctt gttcctgtac cgatatcgcc
    12121 aattgttttg tcagtagagc atttaattgc ttcaactgat cgacatcttc aatatcgtca
    12181 accgctgtgc cactcttctc ttcttcagta gctaactcaa cccactgttt tttatcaaaa
    12241 tcaaatcttg gtctccagtt aggaactggt ggtttaatct ctgtacagtt ttcagggata
    12301 ttttcttgat tgttcaaaat gatttgctcg aatccgtaag gcttgattga tttgtaaact
    12361 actttcatta gatttcctcc cttaaattga ataagtgata acaaatgaat aagctgatcc
    12421 ataacttgaa tttcttctcc atttaatggc tccgtctgcg ccaatagata gttgagcact
    12481 gttcaaagta gaacggtcta tcgagccaac cagttgctca aaactaattg gtggccgata
    12541 gccttctgga attgttagta tcgttgaatc atttccacca ctgctttttc cgtttaaagc
    12601 cacaaaatat atagaaactg tttttccttc acgataaagc tttgctgtcc ctgtattacc
    12661 gttagtgaca accagattta ccacttcata tctgttatca tcagtcgtca aaacacgttt
    12721 attattgatt gttacagaac ctgaaaaatt cttatgtcct gcaatggctt ggtctccatc
    12781 tgtctgcacc aatgcttctt taccatcaat agcttttaca tgcgttttta agtatttagc
    12841 aactccgtct tcttttaatt gaacaatatc agccattaaa ccgtccccac tttctcaaat
    12901 gtaatattgg ctaatccatc aagtttcact ttatcggttg ctgacattaa acctgctgtc
    12961 gtagttgtag cattacctgg atttttctgt gctccagctg caattccatc caacttagtt
    13021 ttatctgtgg acgacatcaa cccatttgct gtagttgtag ctacagctgt agttgtggca
    13081 tttattccag gatcaccttt atctcccttt ggtaaaacaa aattaaatct agctgcagat
    13141 gatgttccta cattcgtaac agaagcggtt gaaccactag aaacggttcc tatggtaatt
    13201 gttgctgctt ggccaggatc gcctttatct cccttcaccg ttgttggttt gccttctata
    13261 gcattccaat gagtttgtgg ataaacctgt acattgcttt gttttatttt tacgatatct
    13321 gtcattctct atacctcccc aattttttca aaagtaaaat ttggaattct ttcgtttgtg
    13381 taattttctg cttgatttac agcttcttgg aatttttgat ctacatatga ctgattgaca
    13441 ccaccagtcc cactaccacc tgtagaacta attgttccat cttcatcaat agtgatgttt
    13501 tctccagcag tataattttt cagtgaatcg agtttttctt ttaactccag cgaaaaattg
    13561 acatcagatt gtctgactgc agagactttc cctgtctcat caatcgtcaa taattcacca
    13621 acttttatgc cacccaaacg gtcgatagtg gcaactggca acacgtagtc accttctcca
    13681 ctgtttaaaa ttttttgata catttctgcc gtgataatgc cgtcatcttc ttcacttgca
    13741 tagggtaatt ctgtgagaac attctctaat cccaaatctt ctttagtaat gacgacggct
    13801 ccaatttttc cattaacaga taaaactgtt gcttctcctg ctattatttc ttctaaccca
    13861 agaactgcgg aagcatgtgt gattggaaag aattggcgtt gtacacccgt tttttcatcc
    13921 gtttccatca ttcgtttagt tttagccatt agatcacccc aactttctca agagtgaaca
    13981 cattttgctt tggatcatca acggttgcga tgatcaaggc tccttcttca atcgggaatg
    14041 ctacagttcc aactttttcg acttcatgat tatctgaaaa gagatcatcc tgcaaaatat
    14101 cagtcacttc aatctccccg tattcaatcg tgaaaagcgt tgctcgcaat ttctgatata
    14161 aatagtccat atctgccagc aaacgctctg aaatagacgc atgacgaact ccttgaatat
    14221 ctactcgcgc atccgttagt tcggctaaca tgactccacc tggatcaatc gtctttaaaa
    14281 tatccttgat tgattcaaac catgccaaat agtctgattc ttgtccttct ctccaatctt
    14341 ggaaactgtg ttcctgttct tgtctccaac gttcaaattc ttctttcctt tcattcatcc
    14401 agtccgtaaa atcgccttta ttttcattaa taaagtccgt catatccgca attaaatctt
    14461 cgattgattg ccaataagaa cccatttctc cttctgtttt tgaaacagca ttaatgacaa
    14521 aataagagaa gtcttgagtt gttccaatca agtcttctcc tttaaatata atgaaattgg
    14581 ctgtttgtcg atgtaaacac tgcatggaat atttatcaaa aatatagtta atttttcctt
    14641 ttttagcatc cacaattttt gtttctaatt ggactggata tttcccacct acaactgatt
    14701 caaaatatac cttacattct gataaatcgt agggaagacc attttcaact atagtcgctt
    14761 ccataacctc tgtattctta ttgccttgtc gaacttgaat catccccaca taattatatg
    14821 gttctgttgt acttaaaacg acattccatt tactcataga atcaccacct taaaattgga
    14881 tataatttct tggattctca aaatcaacaa ctggctgtgg ccaataattt ttcataattt
    14941 gaaaatgtag atgttctcct gtagatggac cagttgttcc catcaagcct atttgagcac
    15001 ctttctggac tttttgtcca acagaaacat caatacgaga caaatgagca taacctgtcc
    15061 acttcccatt attatgttta ataaccacgt aattgccata ccagtcataa taattagcac
    15121 cagcctgaac aacttcccca tctaatgctg caaaaacagg tgtattagga tttccattta
    15181 caagatcaat tccattatga aattcttggc cgccatttat tggactagtt ctccatccac
    15241 attctgaagt aactgtcact ggtttttgaa ttggacaaat gaaattccct ccaggttctg
    15301 gactagttaa tccatgtaat ttgttatacc aagattgggc ataattttgg cgttctggat
    15361 gtgcagaagc tgggcgttcg aagttcattt caaaagcata cgctgcactt ttggcatcat
    15421 tgatcgattt aaatcctgat actgtggttg gatttacttt tccgagccat tgaccgttga
    15481 acatacacca atctaataat tttacttggg cttcaatact tcgataatct tcttgaatcc
    15541 ctgcggtgtt catcaaacgc tgaacatact ctcgtccgtt ccaagttggt gatccgacta
    15601 acggataagc agaaccatcc cattgaacaa tcccataagc tggaccacca agttgttctg
    15661 tatcagggtt catacttgct ccaacctccc cctgaatatt tcctagaata cctgcagctg
    15721 catattcaga atagccacga gctttcagca ttgtccaaat tttccatgct cttttttcag
    15781 cttccgtcgt caattgagga gggatagttc caggatcact tcctccactg ttgttccctg
    15841 tgatttcttt tccgttaact gtgagtttgc cttgtacatc taagtctcca aaataaattg
    15901 ctttaccatt tcctaataaa actaatcctt taccaacttt aggagaaatc aaaatatatt
    15961 tgccgtctcc atttgtacga ataactaaag aattatcttc aataggtgtg ggagtagaag
    16021 ctccaggaaa aggattacca gcagaatcag tagtaccaat tgtgccaatg gattctttgg
    16081 tattccagaa ctccatccct tttttggtta attccatgat ttttttattg ctgtttacaa
    16141 tttgcaaagc tccttttact aacttgagct gatcaccaac agcgttaaat gaagtttcaa
    16201 aaatatctgc tctaatacgt ccagattgga tataattggc attgaagttt ccatcaatcg
    16261 tccatgccgt tttgaaattt gactcattga atttcccatc aataaaacca atcccttccg
    16321 aattagctac aagaaaatgg ttagaagtat caatactatc cccattcatc caaaccatct
    16381 gatatggttg acgactttcg cctttgccag tatcccaagg attcatcatg ataattgatc
    16441 caccctttgc tccgcgaatg atatccgact gccatttaga aacttctgtg gactcataaa
    16501 aggtcatctt cttctcatca agggtctgta tggcagtttg agtatttgaa gcttgacgtg
    16561 ttgatgaagt atttaaatta tcacctaagc cagtttcgac ttttcctgtt aatcgatcaa
    16621 tcttcacact gaaaattcta gtctcgtaat gatagccgcg gtcatcacga tgaatacgta
    16681 taatattacc taaactatct gctccaaaaa tagttgcttt aaattgaact agcggacgtg
    16741 aacagttaac caattcttga taggtaagct gaattagttc aacaggatct tcgcagtctt
    16801 cgaatataat taccttctca cgcttacgac gttttccgtt tttagtagga ataccatatt
    16861 tttcagtcat ctcagggatt tccaaatata tctggccagt aggcttgttt aacggatccc
    16921 cttttgattt ggaccagtat acttgatcaa actcaattcg acgaccataa ccatctccaa
    16981 cctcttcacc acggccgcgg ccgattagag aagtatagat gttacttcga tctacttctt
    17041 tttcaattgt caaggcttta tcgccgtact catatcgctc attactgtat tcgccaatct
    17101 gcttgtaaac ttctatccat ttatccgtga ttccttctcc acttagattg cacctaaaaa
    17161 caatctcgca tcctaacgtt tgtagatttt ttaaggcttc acggatacta cagtagtaaa
    17221 aagtcataga aaccgctggc agagttgaat ctaaatgacc aactcgccac tcacctaatg
    17281 taaaatctat gactctctgg atggttttct gaaaaaattc gttagccgga cgaatatcat
    17341 taatgatata agcatccaat tcatcaggcc caaaattaat cccagtgaat atcaacaatg
    17401 aacccggatc agcaattccg ataattttat acatagaaaa agacgactcg ctttcacgaa
    17461 ccgccatata agctgactct ttaatttcgt catcaaactc catgctaact gctaacttat
    17521 cattaattaa ctcatcttta cttggtgtga tttctttttc ttgaactacc gaaaaaagtt
    17581 tatcttcccc tactatttta ataagctttt ggctatcgtc gaaaaaataa acatctttgt
    17641 ccattacagc cacactcctc tatatcgtat taagggagtg ccattatcac acttaactag
    17701 atcaccagtt ctaaccataa agtttttaaa tgagctggta agatcaagaa ttctcgtttt
    17761 attcactcca ttcacaaaaa cttttccttc agctatgtcc atctcgataa agtcaccttt
    17821 ttttatcatt gagttagtca cactaatatt ttgacggcca tttgtaattc ttaagctccc
    17881 atcattatta gcctttaaac taattgaaat cggagctact tcatacggaa gatattctga
    17941 gactatgccg tttgtttcaa atatacgtgt atattttctg ggatcagaac agattataga
    18001 gaaactagat attacactat gaacgtttcc tggaatatta tcacaggtct gataccttcc
    18061 gtaataatac atatccaact catcgttaaa gtgaatttct acatctttat ccctgtaaag
    18121 cagcctcatc aatttattaa agcttcgttg aaattcaata ggattctttt cttctaattt
    18181 aaaatgtata gttaaaatcc tctctggaag tctttgagaa ctaatacgaa cacctatgct
    18241 caccgcttca ctctcaatct ctaaagaaac catttctcta ccttcaacat acaaagtctg
    18301 gtacccttct atataattct cgataaactt cccatcataa ttcaatgcag acgtcggtat
    18361 ccatgttttc tgactgtcgt ttttaaccgt gtctctgaat tcatacattg gattatgcca
    18421 ctccatcata tttccctcac tttctagaac aataaattaa tatccgcctc tttaccttga
    18481 atgtttgaaa tatcctcaac aaacgcttca aacacttgat ttcctaagcg aatattgaaa
    18541 actgcaggac gtttattttt gccgtagtta acactatgct ctatttgtgt tgtgaaattt
    18601 ctatttgcat tttttaaatt cgctgaaaga tcaatatcag gtgtacgagt aaaactatct
    18661 gcaatcatat tagccatgtt tcctacattg ctttggacac tggcaaagcc attagttaaa
    18721 cctgcgttta gtccattcat gattgcttgc ccagctggga ttaacaattt tctatcgtac
    18781 cgaataggtc ctttgtgttc cctaatccag tcaccaatac ctccaacaaa atcttgcaca
    18841 gatttccatg cattttgtaa cccttctaga aaactatcca tgatagcttt tccggctgct
    18901 agtaaatcga tatttttcaa gttatcaaac cagccagtta ctctatcaac cgtatcacta
    18961 acagcattta ctaaattatc ccatacctct tgagcaccat ttactaaatt gttgaaagta
    19021 tctatagtac cttgttttag gttttcccat ccctgaatga tgttatcctt agttccaata
    19081 actagattaa taaaccaagc tttgaaagaa ttccataaat ctttcgctcc ttgaatcata
    19141 ttattaaaca gatcgattgt cccttgtttt aggttattcc acccctgttt aatactattt
    19201 acaatattgt tagttgtctc tttgatccat gtagtaaaag aattccacac atctttgatg
    19261 gtagaagtca acacattcca gatatttatc acagtatcct ttaaggctgt gtaataacca
    19321 acaactatat ctacaaacgt cgtgataatg ttttggatat ttgtagttaa tgtagtccac
    19381 agcatcgaag catcttcttt taactgatta aaattgcctg ttatcaaatc aatcaaaagt
    19441 agaattggac ccattacagc agtttttata atttcccatg cagaacctgc gattgatcca
    19501 atttgagacc ataggttcgt aaagaaatca atcattggct gaaaaacatt ttttatggca
    19561 ataacatacg gtgttagaat gtttacaatt ccttcccaag cggaactggc agcttctttg
    19621 atgccatccc atatactcga aaagaactct tttgtttcag tccatttatt cttgatccaa
    19681 tctgccgctt tcccaggggc ttcttgaatt gtagtccaaa cattgtctgc gccttcttta
    19741 atggacttcc ataaattgtt aaaccattct cctgtagatt tccatgcatt ctgaatccat
    19801 tctactgccg agcttacagc agacttgatt ccctcccata agccaatcca aaagtttcta
    19861 aaatcttcac tcgtattcca aagatagatg aaacctacaa caagtagtgc taccgcagct
    19921 ataaccaatc cgactggact ggtaagaaaa cctatggcgg aacctaattt cttgaacaaa
    19981 gagattccgt tacctaatac acctaacccc actttcatgg tttgaaacgc tttaaccagc
    20041 attcctaatg catacaatac tggcccaatt gcaatagcga ttgctcctat ggccactact
    20101 aatctttgag ttgattctgg agcacttaca aatttttcta ctaagccgga tatggcatct
    20161 gctacttttt tgatggatgg tgctagaatc ttttgaatta caatagctgc tgactcaaaa
    20221 gctccaaaca tttgctcgat ggaagaattc atattatctt gcatggtccg agccatatcg
    20281 tcagctgcac catcagaatc tttcagagat tttgttaatt tgcccaatga atcaggtcct
    20341 ttatcaatca aagccatcat ccctgataat gattcttgcc catatagtgt tactaaagca
    20401 ttttgttgtt gttcaggcgt caggccttca aaagcttttt taagtaattc tacttgagtt
    20461 tttaaaggtt tcattttacc gtcagcatca taaaacgaaa cacctaaatt atccattgta
    20521 tcttgcatag cctttgttgg ccttgctaac ctagacaatg ctcctcgcaa cgttgtacct
    20581 gcttgagaac ccttaatacc tgcgtcactc ataataccaa tagctgctgc agtttcttcc
    20641 aaagaaatac ccattgaatt ggctacagga gcaacatact tcaatgcctc tcccatgtct
    20701 ccaacttcag cattggtatc cgcagcagca cgagcaaata catcagcgac atgtcctgct
    20761 tcacttgctt ctaaaccaaa tcctctcaaa gcagtagcag tattttcaga agctagagcc
    20821 acatcccctc cagatacagc tgctaagtct aaaagacccg gcattgcttt catgatttct
    20881 tgtgcgctaa atccagcaga agcaagattt tccatgccgg cagccgattc ttttgcgcta
    20941 aaagcagttt ttgctcctag atcaatcgct tgctgtttca tctgttcaaa tgtgtcgcct
    21001 gttgctcctg atatagcttt tacacgactc atttgttctt cgaagtcgcc accaacttta
    21061 gcagctgcta cgcctactcc tataagagga gtggtaatat actttgtcat tgcggcacca
    21121 gtaccttgca tcactttacc tacagcggtt gtcatactat ttgatttctc ttcaaaagtc
    21181 ttaacagcat cttgcgcatc tttaaaagtc tttacaaatc cactatctgt ggcttttaat
    21241 aaggcttcaa cagaaaattg ttccatgatt ttcctccttt cctcaagagt tagctttagt
    21301 tagtaagctt tggaattttt tatcttgttt tgaaagttcg gaaactccca tgattgaatc
    21361 ttcgattttt tgataattga agaattcttc aaaggatcga tatacaggaa ctgtcttttt
    21421 gcctactttt ttctccgctt ggacttgctg atttgcccac gctaattcgt gaatcaactt
    21481 ttctttgtca agccaagata actgggctgc agtcatacga atgttgtatt catataacgt
    21541 cattctttcg atatctgaga tattggtcat ttccaaatat cgaaaagaat tgataagaat
    21601 ttgttcgtat gccagtgcag aatctattcc gcttgttgtt tttccgcttc tttcaatttc
    21661 tgattcaggt ttcggaccgc taactttccc gcgttcgact ccgccaattc ttttaggact
    21721 tcatcaaaca atttttcgat gtctttaact tcatcgatgt aatcatccat ttcatccaac
    21781 gtaatagttt cttcttctgt tctatttgct atttctaaga ctcgtgacaa tgtgttggca
    21841 ttataagaac gtaattccgg taagactttt gctgagagtc ccattccgaa ttccatattt
    21901 ccatcgatga aaggcatcac tttgtctaat tcacgtacaa atttagtgcc aaatttaaac
    21961 gaatattctt tacctttaat ttttaatttc aatgtttttc atcctcctaa aataaaaaag
    22021 agagcatcta agccctctta tgctcctgtc gaagttgctt tcacggtatc tttgaatgca
    22081 tattgaacaa catcggcttg atcttctgtc aaggttgcat aaccatcttg accaacacca
    22141 tttactgcaa atgataaact taattcaacg ttatcctctg cagcagctga tggagtaaat
    22201 tcagacacat atgcttggta ataagtagct ttgtacttat ttgcatcatc atctgtgccc
    22261 tgttctgctt tgttgatttc ccaaatttca atgatatcgc catttaataa ggcttgtttc
    22321 atttcatcta catgagaatc gcctttggca actattgaag tagccgaaaa atcatattca
    22381 actgggctta aactttgaac gtttccgtct tttgtcactg tagagtctga atctcttgat
    22441 aaaccatttt cgtgttctgt ttgaaatgcc attttccaag cagcttcctg agtttctttt
    22501 ttcaataagc gataaagcaa aatgacatca atacctttta atgcttccat gttcttcctc
    22561 ctatctaatt ctaaattcaa gtgtgacaac cgctcgtttt aggggcgtat tggttgttgt
    22621 gtcgtccatc acttgaattc cacttgcttg ataatttaaa tcccaataat agccttctgt
    22681 ggcttgtatc tgcaacgctt gattaaatag tgccgaagcc atatcagaca cttgtttgcg
    22741 ttttttctgc aatccccaaa cggaaattgt caaattgact gttcctttga tgtcggtttt
    22801 attcggattg tggactgttt gggagctttc caactcaaca aacggataac ctacatcgtc
    22861 taatggtttg tagtcgtaag tttggtatcc aagagctagg ctcctagcaa acatttcatc
    22921 aaataatgat tgatctcttg ttttaatcat ttgaataacc tctgcatgtc tttcataaag
    22981 attgctttct gcacattaaa tgcgggacca acgaaaggtt gagcgctcat gaatcgagtt
    23041 ccatattcta agtacggact gtaatcactc tgcggtccaa cttgtgcaac aaaaccgcct
    23101 tccaacaacc acattcgaat tgatcgtcta gtgaatcctg tcgggtgcac aaacacatcc
    23161 ccttcccaat gtccattaaa gcgggctttt tcttgcatgc gcttagtaag ctcggctcca
    23221 tttgttttaa ctaccatttg cacatctcta agcgtgacag cttcttccaa gtgtttaagc
    23281 agctcatcca cacctttgta acccaatgaa actttcactt tgcaatcacc tcctggacga
    23341 taatgctatt cgttgataac aggctgcgtt gagcgacatc tttgtatgtc tttccattaa
    23401 aaataatgcg gtcatatttt ggaacaacaa aaagaggcat ggttctgata accttcgccc
    23461 cttgtcgtat atccccaaaa atcgccacac ttctatctgt tcctaagtcc gttacatttg
    23521 cgtttgttgt taccgtaatc ggtgcttctt ctatccactc gcctaattct ggatcgtaac
    23581 gatttccttc tccatctttt tcaaagataa cctcatctaa aaatctcata agaaccgcac
    23641 gctccctttt ctaggtttgt acaattcttc gtcatctttc cgtttgaaat catcaatttc
    23701 cgatgcgtat tctgaaaaat cggaatcagg gaaagccatc gataaaccct cttgagcgta
    23761 tgaagtcatg ccctcttgcc caattcggtt aaaacgtttg ttcgttacgt caaaaacgat
    23821 gtattctagt gattctggtg taacagaaac gcctagcaat gacgctaagc gctctctagt
    23881 tcgtctctcg ataacttcta gcttatcatc tgtagaaccg cttagaagct tcttaacgtc
    23941 atctttaatt accattcgta cctatccttc ctacggctca gtcagtgtca aaacgtgagt
    24001 atctgtgtgt gatccatctt ccgtttttat tgttgtcgtg tatttaccgg ctggtactgt
    24061 gtcagtccaa gtgatattac cgctagaaga aaccgtcaat ccgcttgtta ctggcgcaat
    24121 tgaatacgtc actgttttat ttgttgcgtt ttgtggtgca acggtagctg ttaattgtct
    24181 attgccagca gtccccgcaa ctgcgcttga ggttttaggc gttacagtaa cgcctataac
    24241 ggggattgtt actcccccgc cggttcaatt ttaccgaacg cagcatcttt gacgatcata
    24301 aagccgacat ccattgttgc acgcaacgca accagctctt gttcaaacaa gtttacagga
    24361 gtgccatctt cgtttttaag cgtagataat tgcgcatctt ctgaaatctt gaacgaaatg
    24421 ttgaatggga taccatagaa catatagtta aagtcaccag cgtacaattc acctttaccc
    24481 aatgctttca agtcaaccac aggaagacca tcaatcgtat tcgccgagcg gtcatagata
    24541 aattcaacgt ttgtaccgac tgtttgagcg gcagaacgca attctgtgcg gtttttacga
    24601 tttgagatga acgcatttgg ttcaaattca tttttgccta aagcatcttc taatgccaaa
    24661 atgttgtcat aagtcaaagg acctttgata acattagccg ctgcaacgac agattcttct
    24721 aaagactgtg gaaacggatt gtcagtgttt agtaatgcag ccgcatcaaa ctttttgtag
    24781 aaagcttcag caattttagg ttgcataaca gtgaagaaat ctgacatttt gtagtgcaag
    24841 tattcacgag acacaggaat aatcacgcct agctttttag ctaccattgt cgcttgcaac
    24901 catttaggtt tagatgtttg aatcttctca ccttcaccaa cccagtatgc gccaggacct
    24961 tcagcaaagt attcaaattt cttttcctta tctgtcattt cttcgtattt cgccaattgc
    25021 attactttcg agttttccat aatgtcgttg ataatcaaag tattatactt gtcagggatt
    25081 ttaccctctt tagtttcata aactgttacg ttatctggat tccatgtttg tgccatattg
    25141 tttattcctc ctatttgata attcgatttt tagcagctag tttagccact gtttctttag
    25201 tatttttcga tgctgtaaat tgtccacctt catttggtgg tgtttgtctt gcgttttctc
    25261 tcttaatcaa agaagcaaag ttagtgatga ctgctacagc ttgttttgtg gcatctgcat
    25321 catcagaaac aatcagacca agtaaatcat catcgtgtgg taaatttgca tctgtcagca
    25381 ttttagaagc ttctttcgtc atttcagata gtgcctgtcc acgctttaat tcagcgattt
    25441 cagcttcttt ttgttccaac tcatgctgta gtttttcttc cgcattcatt tttgccagct
    25501 ttttagcttc ttcttttttt gcttctagtt ctttttccca cgctgctttt gctttattcg
    25561 tctcagcagc gatcattttc gctacttcat cacgagaaaa tgttttgcca gtattgtttt
    25621 cttcttttgc ttcggtcggt gtctcttgtg agccagctgg taggtttcct tgttgtccct
    25681 catcaccaga tccaccatct cctggttcag aaaaaaattg taagttcatt ggcataaata
    25741 aacgtttttt catgattaat cctccacggt tacgccgcta cccgataaat ttgaccagtt
    25801 acgccggtca gtcggaacag ctcaatttat attgccccga gcagtagagg gcataataaa
    25861 aagcccgtta ttcggcttca cttacatctc ctgaaggtaa atatttcacg ctgatatcaa
    25921 tcgattgaat accgtcttcc cattcactat cagtttcaac ggatactctt agcacttttt
    25981 cgttttgtag aatcttattt aaacttgcga tagctctaac aattgcttct tcattttcca
    26041 tatgcgtact ctcctctaca tagaagcgct ttggaacacg ctttgattca tattgttttg
    26101 caaagctttg aagtactctg ggttagactg aaccaattcg gttattgatt gcacgacagg
    26161 ttcaataacc tttctagccg tttcatacac ttcttttacg attggttcta agacatcaat
    26221 taaatgctgt ttaacggcat catagtcttc tttaaattga gcatcatttt gatacaaatg
    26281 caaaatttcc tctttgcttt tcccatatgc cgtttctaaa aatctgtcag atataacttg
    26341 aaatgaatct ttcttcattg ttataacctt ctttctttaa gtgacttctc aaaagcatca
    26401 cgatcaacaa tcggataagt agaacatcta tcaaaaggat gtactggcat catattctta
    26461 cctacttcca tttcactcac taaatatgga ccgtcagcgg caatgctctt acataatcgg
    26521 caagcattag gttcagttac ccatccatat ttctcaatat tcgcatctaa gtaactttcc
    26581 ctctgtatac cgctttgaac tcttgttgtt tcagttacca ttaaacgttg cgtattaaac
    26641 ttagtatttt ctcgtccttc ttctgttaga aacttactta attctggagc gagctgttta
    26701 ggattacgtc ccatcgtcac acttcgaaca agcaacttat ccaaatctgc tttcaactct
    26761 gcttggtaca tccatagcct ttcgctgaac gttgcaaatc catccgctcg aaacgagctg
    26821 tttatcactt gctccactaa ttttgcataa ccgcttttag caatcgccat ttctaagatg
    26881 ccagcttgcc gcttaagctc gtctagccct gcttttgtaa gttcattcga gaaatactta
    26941 tctagctcgt taaacgtagc tataagctct aaaccaatat tggcttttaa caattccagt
    27001 cgattgacac gcatcgttag attgtatagc ttcaattctt tgttcgctgt aggtgagaag
    27061 tctttctctt taacatactt cttagccttt ctagcaaatg cttttacatc catttcatta
    27121 gcacgcttca ttgcttcgct acgagtaatt ttctgcccgt ttgaaaaact attccattgt
    27181 gcgtctatct ctttctgtat cgcatcctgc gcgtattgca gacgcttctt gatctcattc
    27241 atgcgtttct tgtcatcttt aatctgttgc tcttgccaag ctttctccct tttgatgaag
    27301 taatcttgtg atttcactta atcactcctc gatgatttcg taggtctcaa taaagatatc
    27361 tggtttgcac ggataaaatt caccatgaac gcctttgatg atgtaatcac catcagatat
    27421 ttccattgtg ccttctaatg tttcaatacc aggtaatcca ttgggcatat tattatggtc
    27481 aatccatgct tcaccaacga attctttgca tttgcgatat gaacgtgcag atagcatttt
    27541 aagttgcaca atctcgacag taactggttt tttacgtgct ttcatcttct tcaacctccg
    27601 tatcagtttc tttgtcactg tcaaacacac ctctgtccgc tgacgtttca gcattcacac
    27661 gttttaattc tgcctgtaca tctggaacaa aagaagcgag tcctaagatc gtctcttgac
    27721 tgagttcagc tccagcatca accaaagatt tcaactcttc cagaatggct ttaggtagat
    27781 taggcgtaaa tattacacgt aagcctttca aatcggagtt atccatttca gaaatacttg
    27841 attttaggct aaacaaaaga cgataacgcc gcataagacc ctttttgaat agcctttgct
    27901 ttgttgccgt catttgttca aatccaaata atttatattt catcgcttct cctgattgca
    27961 ctccggaaaa attgtcatca gtaagatcag gaaccattga gatttcgtgg atatccttgc
    28021 gtactctgtc tttgtatgct tctacaccgt tcacatcata ttgtttgtaa atatatcctg
    28081 cagtcacact tgttttatta ccgttcacat cagttccaga ttcaagcaaa agcatattcg
    28141 cttctttctg cttgatggcg tcctctgtgg atagtcctgc tgcttcaata tcaccactaa
    28201 taactagaag agcatcgttt agatcagtca tatagttggc ggtatcagac tgccctgcat
    28261 cgtatagatc aatcaaagat agtacatctt catacaagcc catccgaaaa cgattaggag
    28321 aatactctgt aataggtacc tctttatatt catgcggttc atcctgggga ttttttaact
    28381 caattgctgt tagtgtcgtc tcatcataag tgatactttt ttcttttgtg tatacgattg
    28441 gttgaatgta ctgtttatca gcatccttgt tgaatcttgt cttaggatac cgtacagcca
    28501 aaataggctc tcgttttact gtagtatcat atacaacaaa tgtttcaaat acattagcca
    28561 aatcaacata atctgtatca tctgaatctc gatagataat ctcataggct ctcccatact
    28621 tatccatatc aagccagagt tcagcattta acccatctat gtcattatta gtattaaact
    28681 cttcgatttc tttttgttga tttgtatcct cgatttgcac ttttatggga ttgcctgtgt
    28741 tgtacccaac atcaaacgta caaagaactt ttccaaagtt atgtgctgat cggtgatccg
    28801 ctttttcctt ttctctacgt ctacggttat ccatgatatt tgtatttcta gctttgtaat
    28861 aatcatctaa tacactaagc cgctttacct gatattcatg atgatgtttt atcattgctg
    28921 ctaaagtatt tgaatcgtta agtaaatctt ctgctgagct aaatctatag tgaagatttg
    28981 attctacgct aaactttaca taatttgtgt tcacgtcgtt cgaataatgt atatcagtac
    29041 cgtattcaaa ttcgtttacc ttatccattt ctcacactcc ttaaaacata cgtttaacac
    29101 gtttaatctt ttcgttgaca ttaacttttg gtttcttaag aagctcatga gtatatatcg
    29161 catatcgaac ggagtctagc acatcatcat attcttttat tggctcacct tttttcttgt
    29221 cccaaatgta ttgataaatt tcatcccgaa atttcatcac tctatcttga cagataaaca
    29281 atttatcttg tttaaatctc ttggcgactt cctcaactcc agataaccgt gctttatgag
    29341 catttttagc attaatcttt tctcttacaa acctagccac atgctccggt ctcgcagagt
    29401 cacaataaaa aggcactcgt aagccgtaac gctcttgaat gccttttgct atatctaccc
    29461 aataatcaat ctcttcaaat tgagtagcat gttcttcgat taaataagct gttccgtcat
    29521 ccgtttcacc tataactacg attgaacccc agtgttcata tccccagtca acaccacaat
    29581 aaaagttgct caaaggtggt aagtcttttg actggatgta gtgcttgcta gcatcaaaat
    29641 cacgataaac gacaccttct gcagaaaccc ataaaccttt gatatcacga tcataaaaca
    29701 tgccgcttgg tgttgattct ttaatattat ttctatagcg ttcagataaa aaagtattat
    29761 catccaattc aaagtggaac gattgaatgt ttttacttga attgtcgata tactctttct
    29821 tcagccagtg ttccgggttg tcagggtttg tgtcagctag gattctcgct cctgttcctg
    29881 aacaacgtga aacgatttca gcaaatactt cttgtctagc tagtgatgcc tcattgatat
    29941 aagcgccata tgctgtcata ccacgaattg caccaacacc gccaatgttt cctgtataag
    30001 cttgtacgac cttaacgcca aataacttaa agtttccatg tttatcgaat ttaggttcta
    30061 tgctgtacat gttgtatagt tcctgtaaga tgttcttttg gattgttgca cttgaaactc
    30121 ctgctaggat atacattggt tccttaatgt cttcttcatc ggcaatcttt cgcacacgtc
    30181 gcaattcaaa caagaacaaa tcattgttta tttttgtttt cccagaacgt tttgctccat
    30241 gtaataaagt aataaaccaa tcattcttaa tggttttatt taacacgtcg atttgctttg
    30301 ggttataaat atcaactagt cccatctaat tcaccactaa tcttttctaa caactcatca
    30361 agtttttctt cggtagaacg ctctgagttt atctgtaatt gttctaattt tgcttgcatt
    30421 aattttattt ctgtttgagc tttttctaat tgcgtttgtg ataacagcga tttatacttc
    30481 aaatacaaat ccaaggcttt caatttgctt tctaaatctg gtgtatattc atattgcata
    30541 tgtttaataa ccttgttttt ctccaagcgg tctatttgct tgctcacgct tatttgtact
    30601 tctccttgcc aaatatctat caattcattt aaagcgtcct cagcacttaa tttgcgcttc
    30661 ttttcgatag gatttagccg ttcattaata tactgtatta tgttaggttt agttaggttt
    30721 tccttaccta ttactcttgc tgatcgtttg ctataacctg ctttaatggc ggcttgagta
    30781 gcattaccac cattttttat atattcatcc gcaaaagctt tttgttttgg tgtcaaattt
    30841 accacattca agccaccacc tttcaatttt attaacaata tctctcaata tttgtctgta
    30901 tgttctgctc actaaaatat ccatggccac agtaacgaag attgtactta tcgatctctt
    30961 tcggtgtagc ttctctggtc atttcaatga tggagtactt ctttttgatt tggactgatt
    31021 ggacaactct aattggatca tctgcgtttg gttgcggata tttatttgat aatgatacat
    31081 accagtagtt tctcattatg tatcactctc tctgttggtt attggcagaa aaggttcgca
    31141 tcataaattc catagcctgt ccttcattaa acccttgtag aataagctga tcgtagaaat
    31201 acttagcttg ttttgcgatt aacgctaagc ttttttgagt ttcatagaat gtcgcctcca
    31261 tggtttggtt tgcttcattc gttttaaata actctcttaa ttggttttca ttcatagata
    31321 aaactccttt caaaataaaa agaccactca atgagtgatc taatatgtaa tacaggaccc
    31381 actatccttc gagggaatgt ggattacaga aacatcagcg cgctatagtt tccaacctgt
    31441 atattcggtt ttactgacag gcagttatgg atcaccgtaa accaaagtca ctggcaatga
    31501 atcgaacatt gcaacaccat tttccgatat tttacagact ctccatcata aaaactctca
    31561 cctctagaat tgtctatctt taggggattt atttcaagat gcctcttgtc ctttaccctc
    31621 gacggtctct tgcctagctg cataaactaa gctcaggtgc tcattaccac tgatttaagt
    31681 tattagcgtt acctttccgc cacagtgaca aatttatatt gtgaaaataa atactaagcg
    31741 tataatttta attatcagcg agtggtccgc tgaaataaat tataggtggt gaaaaatttg
    31801 tactttgtta taagagaagc aactaatggc caatattact tcgttattaa gtccaataac
    31861 aatgaagtag tagcaactag cgaaacatac ttgactaaat attccgctga acaaaccatt
    31921 aattctataa aaaacggaat caccaaagac tctcaagtta tcgatatgac taaataagac
    31981 gactaacttc atttgctagg gagttcattt cagctgcttt ttctaccaaa gcagttgaga
    32041 tactttctag cttttctgat ttaacagaat taaatactgg cgtcttgata ctaatcgata
    32101 catcttgacg aggatcagga ctcgttgctc tatttttttg caccactgat ctgtactttt
    32161 ctactgaatg atactcaatt acttgttcta aatgtttttt gtactcttta catacagctt
    32221 ctttttggat caattctaaa cgttgttgct caataatatc aatcaaccgt tttcgatcca
    32281 taccttgata ctttatttgt tcgacttggt cactagcctt gttgagcagt tcttttagtt
    32341 cagtcaaatc taatttgaca gttgctttga tttctttatc cattcgtttt ccctccaata
    32401 cataaattaa tagacagcaa cggatgatag ataataagaa caatcagaag gagttgaaat
    32461 tcacatcctt attcttaata tttccgttgc tgtctatcga agcttaattg tgaaacaata
    32521 ataaaacgat gttcctttta ttattatttt gtctcagacc tatcactaat ctttcgacac
    32581 taccataata tcacgttaaa acactcaaaa accctacact atccctacaa aaaccctaca
    32641 aaatcaacga tactgaacta atacgccttt tttgtatgct tcagcaaatt cgatcaatgc
    32701 gatggatttc agcttctcta cattcttctc tccgtatcct cgtatcaatt gacctatttc
    32761 ataattagag tgcttattta cgtcacagaa gctgtagtag agtatctgac gactaatcag
    32821 actgagagcc atcaaagccg ctaaaatcgc gtctctctct gcttctatat ccatcatttg
    32881 aatgatcgcg tcttctgcct tattgacgtg ctttggtgcc ttcggcatat cggttatgat
    32941 aggcgactta atgtctatca aagagcgacc tgccatccgc tccaagcgcc gaaagttctt
    33001 cagcacatct ctcgcattac atcttgtctg tttgaaatct acctctcgta acaattgcat
    33061 caagtcaaac cgctccttta tgtgatataa taaacttgtg gaatttatta gatcagtcgg
    33121 agcgatccgg cttttttatt tgtcattgat tagtttaata tccaccaatc tcgctacagc
    33181 taaattctct ttgcttttcg ctaaccactt gtcgcattcc atcgtgtttt caatacgaat
    33241 gatcgctgag tgattataaa catgttctac atatccacga aatggataga tgaatccttc
    33301 tgcttcacat cgaaccatgt cgccgacttt gaattttggt ttcttacgtg ttttagggtt
    33361 ctttgtcggc atatctagca ttaaaccgcc gatgccgtgg ctgctggagt aaaatccgtc
    33421 ttttagtttc attccgcttc ctcctgtttt aattttcacc atttctttgt tgaaaacccg
    33481 caacgtctat actgtttaat tcgtttctta ggtctttcta gatcaaccag aataaatctg
    33541 tcggatatat gataatcttc tattacagcc gtatacattg gattcgaaac cgagtaccat
    33601 gatctttttt tatcaaactc atccgcatca tctcttgtaa tttctatcat gttagcagac
    33661 attagatagc tctcttcttc tgcctcgaat gggcaggaat attcatccca gcttcgagga
    33721 aacctggcca aatctctttt taaataaact tgaacattca ttccgcttcc tcctcatcat
    33781 agaaaatcat tccacccaag acctacgcta tgcataaagc aaaattcgtt acacaataga
    33841 tgtcttttat ggtattcatt cttaaaactc aatctgtttc cacattctga acaataaagt
    33901 ttcactttct tatctggata accgtttttg attaactgct ttttaaattg tttatttttt
    33961 tggcgtttat tcattccgct tcctcctgtt ttaatcccca ttgagcgaat gctgctagaa
    34021 cttcaaattc ttctttcata gtaaaatcat cataaaaacc aaaaatattc gtcggcatat
    34081 cgtttgctgc atgctttaac acataaaaag gaaacggcca ttgtgatttt gcgtaatatt
    34141 cttctaacca ctccaacacg attttctggt tgtcgttgag cgcttgtcca tgagtgagaa
    34201 ctttacccga ttcccaatct cctgtttttc tagcatattc atattgttct gctgttaagt
    34261 aatcgctcat ctttctacca cctcttccac tggcacagca aatggccagt atctttcatc
    34321 aatttctttg atttccgctt ctgttagctg ataagcagat ttttcccaag tacatagcga
    34381 gcgactaata tcaaaacaga aatcatttct gttattaaat ttcttgataa gatataagtc
    34441 accaataata acttcataca acggttcttt ctcaacctcg tagccgttgt ataggctcaa
    34501 tagtgtttca tatgattgtc tcgttgccca agcgagtaat tcttctcctt gttcttgcgt
    34561 caatatgcca tctcgcacaa accaatcaca gaaataatat gaatcaccat ctttggattt
    34621 gattaaatac gctatttttt cagcttttgt taaattgtcg gtactttttt cgatccaacc
    34681 tgccacgagt tgtggaataa ctggtttctg cgattcattt agttgttgga ttagttctaa
    34741 tgcgtgttct aaacccgcat attttccagc atgaaaagta cttatacctt taaagtcctc
    34801 tatgctacct acaaggcttg atagtttatc aatcaattcc tgtttattca tcgctgttcc
    34861 tccaataact cgctattctc gtatatattc ccgatgactt ccaaaggtga atctacaaag
    34921 tacaaaaaat caaattccgt gccgagatct ttgtaatcaa cacaaaatgc gccttcttca
    34981 aactttatcg ttgaaaaagt tactttcaaa tcagcatcga gagttttgac aatatcccct
    35041 tcaaatatat ccacaccatt cttatctttc attcctgtgg attgcatgag ttcaatatca
    35101 agaaaatcaa taatttcttc aaactgctta tcttcttcta aatcttccct caaaactaaa
    35161 caattgccat aaataaaatt aggtaatgcc tcagcaatca ccccattaag atgattgctt
    35221 aaaatatcag gttttaccca cgctctaaac ttcggtatca ttcgctgtcc tcctcgtatt
    35281 tttcaatcaa ttccattact tttttcacta tttcaaactc aaccgctttt gattcttcga
    35341 aatcatgaac aatttctgga aacagtacat catcaactac ccacaaaata atctggcgct
    35401 ttccaccaaa atttatgatt agatggtccg attccacaga tagtgttgct cctgattcga
    35461 tatcatataa atccatgctg aattgaacga gtttttttat catttgctgt cctcctcaaa
    35521 tactcttcta atatttcttt atacttctct acaaatttga aacgatcttg atgaagtttc
    35581 ttgctccaat ttgtttgccg atccagctca cgcatctgat cgaacccttt ttgaatttcg
    35641 ttgtaataaa attcaatgtt tgctgctgct ttccaatgcc tgctacttcg cactcctgct
    35701 cctgtttcag ccatttctaa cttaactaat tcagctcgtt cttttgattt tttgtctttc
    35761 tgaatcttca tcatgatttt cttgaggatg atgtcactgt attgtgtaat gagatccatt
    35821 atctctcctc cacataccta aactgtcgtc cttttgaatc aatccataag ctcctagctc
    35881 tatcccagat aatgtttttg cttaatccag taatttcaga taactgttca gctgtacctg
    35941 ttactagaat tcgatcacca tgccagattg caatttttct cggcgttttc cgtttaggct
    36001 tttcagtcca cattgattta ccgagctttt ggacttctgc aactatttct ttgtcttcct
    36061 gccaagattc tgacttggtt aattcagcaa ttcgtttcat tgtcgctttc ttatccacgc
    36121 tcattcctcc aatctacgaa tttcccttct taagttctct atgtgcaaat cgattgcctt
    36181 cctcgccgtt tcattgacca tcactgcctt tgttcgttcc agatcgtcaa tctcacgctg
    36241 aaggcttcga atacgcattt gaatcacttc ttctgttgtc atgatggacc acctcgctaa
    36301 aaacgctctt ccttgaacgt attccgatat tttttagcta atatcaacgg cgcttgatat
    36361 tgatgacaga acaactttgc cttgatctta aagtcttttg tctgcattcc tttgacatct
    36421 acgactttga caagtttgcc gtttttataa aatgtgaagt cgggaatata ctcgatcttg
    36481 cgatacttct ttccgtctag ttcaaatttc ggcatcagct caaatctttc ctgaagtttt
    36541 actttccagc catttgcttc agcttgccac aaggctagat cgtaatactc tgcttctgcg
    36601 atagaatcaa acttgatacc tcgatgaaca gtttttttat tacggtattt attcatgcga
    36661 tactaccttt cactggtttt atgcgcttgt ctgctgtttg ttggaatttc agcgcataac
    36721 cttctgaatt cttaaatatc ctagaaacaa ttctttcacc gtaggcttct cttagttcag
    36781 gaccagataa gtttgttgtg atgatcgttg ccttgttctg tctggcttct aagagcgtgt
    36841 ttaacgtgtt gtttgtaaac tgcctactat ttgatacccc actacctaat tcagctccaa
    36901 tatcgtcaaa aaccaccaaa tcagttgttt taatatcggc tataagcgat ccttcaattt
    36961 cttttctcag ttcagtattg ttataagaaa actttatttg ctctaataat tcttgatagc
    37021 ttataaaaag tattttcttg tcataatttg agcgctcaag tatttcccaa gctgtggcca
    37081 ttgataagtg gctttttccg cttcctgatt tccctgatag aatgaaatgt gcaggatggt
    37141 tcagcagaac ctcattcgta tagctttttg ctttttctaa agcgattttt gtttcttgat
    37201 ccactacgtg ataattttcc attttgcatt taaacaaagt tttatctgtt aataccgaac
    37261 cattttgaaa aaaactcaac gctcgtgctt ttaagctatc gttatatatc cgttcggtct
    37321 gtatatcctc tttcacacgt aacgctttat aaccacaact catgcatgtt ggtttacaac
    37381 gttctgaacc atccttattt ttagctcgcc aactatacaa aggttcgcta cattctggac
    37441 attttccgct ttgcactaat actcttctta ttagtttctc catagcattt gctaggcttt
    37501 ccatgtgatg catctccttt ttaaattggc aagtcgtcat attcactagg attgctgtac
    37561 tgtagttttt gactttgctt tttatgattc ttcttgtctg ctttgatttc gaatttgagc
    37621 ttctcaaatt tttctctcaa tttcttagca cttctaatat ttccaaacca aaattcattt
    37681 gtaggtagcc aattgatcac atactcaatc gcttctatag atgctttatc tctttcttcc
    37741 atcaacctga ttgtgtctgc ccatttttcg atatctactt tgttcatttc ttttggaaaa
    37801 tcttcagtta aattactttg caacttttta gcaaggcgta agtgttcgtc agaatactta
    37861 cctttctttt cttctttatc tatatcttta tcttcttcta tatctttatc tgtaccgtca
    37921 cgtgacgtca cgctaacgtc attttccaat ttgagacgtt cctgtctctt tctttccctg
    37981 tatttacggt ttctttcagc attttttagc cttactttat ccataccctc gatattttga
    38041 tgtttttccc aattactgat ggcaattagt ccatcactgc ttagatcaat catgttgaaa
    38101 tttgccaatg tagttagcgc taagcgaacc gtatttacgt ttttgccaaa caatgtagca
    38161 agcatttctt cggtataagg catgttcctc tggatatata tcagaccatc gtcgttagtc
    38221 tttcctgcta aaactagtaa tcgaatccat ataacgatga tggcatccga ctcaggaaca
    38281 gcttggatta atcgtatttt ttcatcgtca aacatagtag ttttaagttt gatccaactt
    38341 atctcagcca aatttatcct cctatcctta actttttaat tgtttcctgg tttaacttga
    38401 tccctttgat ttgatattta tttttgaaat tgatcacacc tattttgtgt ttctctgtgt
    38461 gatggattct gcagagtgct gcaaatgtgt actctgaatg atcaacttct ttgcgctttc
    38521 gtcttcccag cgctttgtca aagtgatcga tatcagctcc tgttttgcca cagatgcagc
    38581 agactctttt tgtaatgcat ttgtagaagt aatactcttg attcgctggt aaaatctcat
    38641 atccttcttt gaaaggaata tgatgttcaa agatgaaatc taggatgata ttcgctaaga
    38701 tattggcatc actcacggtt gtattcgatt cgtctttcag gcttattttg cgccctgtga
    38761 caccttcaaa acgaaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga
    38821 taaaaatatc gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag
    38881 gatcaataaa acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta
    38941 gtcgatcaat gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt
    39001 tcagaaccgc tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa
    39061 ttcttttctc ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg
    39121 ttcaatagtt tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata
    39181 ttcgattaag gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt
    39241 ctctagcatg tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg
    39301 aaatacatat aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga
    39361 gttattgatt tgcattgcat ttggtttaac aactggttgg tttcgataat ctaaaacagg
    39421 aaataattcg gtttccgtgt gtcctttaac cgttactgag acagatacat aagtcccagt
    39481 ttcatccata agaaaaggtt tatattcctc aacaagaaag tcttgatgag ttccagaaac
    39541 aaccctgtag tgtttgtact cattaatagt taccgttgcc tgtggatcat tctttttcat
    39601 aatctcccac gcgtgagccc aagataaata atcaaaatct ccttttttct tgagaatttt
    39661 atttaactta cgactaaaaa gtttttcaaa gttcgttgtc cctttgattt cactcatcaa
    39721 attctgcctc catttcagca atgtatttct tacctggtcc gtaataagag atatcaatca
    39781 agttatctct gtcgtactct tctagcgcat caatcaagcc atcttcgatg acatagatat
    39841 actcaggttt tttggacttc ctcgataaat ggataagata gacatgatcc caaatactca
    39901 caaaatttcc caagtcatct tgatcacatg ctagttcttc atccgtcaag agatttcgtc
    39961 tgatttttcg attatttgtt tccttgatat tcgatttgcc ccaactagga tcagtcaaat
    40021 attgatctag agtggaaagt tcattttcca tatgttaaaa tctccttagt tatgattttt
    40081 tgagtgactc attgctttgg tcggcggagt cactttttta tttgttgcca tgctttttgc
    40141 ttttcgatat gttgcttgct taaaataata ggacggctat ttgcccacca attatcagca
    40201 atcactttac cgatttttag cgcttcttct cgtgccatag ttgctccttt cttttgaatc
    40261 aagcagattg attaaaacca tcaatgctgc gaacaaactt cccccgataa tactttggtg
    40321 tactactatc actaatagcc ctaggatgaa tcctataaaa agtgtgtctg tcttcttcat
    40381 aatctaatct ccctattttt tatttctagc attctcaaat cctcaagttc agaagcgatt
    40441 agttcagctt gtctatctga tagctcatcg gcttttctaa gcgcttcacg atcatcttgt
    40501 aattgtttcc tgcgttgttt aatcaaacgg agaatttgat gttcttgttg caatgtgtag
    40561 gacataaaat cattctcctt tgcctttaga actcaaagtt ttctttcaaa aatctttgga
    40621 gttccgatcg ttcaattctg atgtctaact tgctccactg ctgtgttttt aagcctaggt
    40681 ttatccaatg tgttaatttg tcatcaccaa ttcctaaaac ttttcttacc tctgatttgt
    40741 ttggatatgg aggaagctct actgatttgt tcataaggtg caatcgttcg tccaatgaat
    40801 taagcacagc attcgtaatt tgtgtagtta attctgaaac tactaaatta tctggaattg
    40861 ttatttgcat gctttttctc cttttctaat tctgctagta ctgcctcaat tggcttgatt
    40921 tgtttatctg gttttctacg cccgttcata atatccgaca tataggctgt tgaaatatca
    40981 agcttttcag ctaaccaagc ttgactcttg ttatgcgtag ctagcgccac acgcactttt
    41041 aaaatgaagt cctgcgacat aactatctct cctctaatag atcaatttct ggaatatatc
    41101 cttctttttt tagcgactca taaatgaaca aacgtccttt ttgagtccat tttgtattca
    41161 tcacaacttt tgttccacca tcagatttcg gaatctcagt tgtatgagat tttgtatatc
    41221 cttgtctcat atgtttcttg cataataacc attggttgcc tacttttttc tgaataccta
    41281 gtttatgaag tagtttgttc atctgttgtg gagacatccc ataatctgct gcaatctgac
    41341 taattgttac tgaatctgta gaagataaga tactatccaa atacgaaatt ttgggttcgt
    41401 attctgcaat tttttgttct gcgattagtc ttccagtacg ttcttctttc aacttagttg
    41461 ctaattggat gattgtatct ggattaagca aggcttcttc tactttttct ggagttagat
    41521 aacctccatg ttttctaatt gctggcaaca cttcacttgt tacccatcgt ttgaattttt
    41581 tggcagaagg aagttttgat tttaagatta aactgtaaag gcctgactcg ttgatgattg
    41641 tcatattacg attttgacct gatgcactaa ttcggtgcat tagcctatcc tcttcgtcaa
    41701 catgatttct gacagcgttg tctgcacgtt cgtatccaag aatctcagct acatctttac
    41761 ctacaaaata cggttcatca tttaataaaa ctgttcttac ttcctgttgt ccgaaattaa
    41821 aaatttgtgg tgtgttcatt ttgctcattc ctttctttgg tataattttg aatagaaagc
    41881 gaggtgaaaa tagtatggaa gaatttaata tggatgtcga cgccttattc aaacaaaccg
    41941 tattcaagac tgttaataaa gaattcaaaa tagatttcca aaatgacgaa tcttttccaa
    42001 cagaaattga attattcgaa gaactttctc agagcgtttc tgagtctttt tctcgccagt
    42061 tgcaaaagaa tttttttgat gctctcgtgg atgaatttca tcaacaacag cactaaactt
    42121 ttggtcttta aaatttaaag ttattaaagc tccttttgcg gaaggagttt ttttattttg
    42181 tttcatgatg tttcctcctt ttctttaaat atgtaagcta acaaaattag ctaattttgt
    42241 tgacagtttc tacaaaattt tgtagaataa gtgcatagtt aaataagcac agaattaccc
    42301 tataaattaa cattctaagt ttcccgacct tgaatttgtt tactttatta ggtatctttc
    42361 ttattgcttg ttagcttatt aaattagctt acgaacatat attactataa agttttgtag
    42421 atgtcaacga ttatctacaa agtttttaag atgtgtttta ggcatgatcg gagaatcatt
    42481 attatgacaa catttgagag agtaaaaatg ttagcagata aacgcaaaat atctattgtc
    42541 gaattggaag aaaaacttaa ttttagtaaa aattcacttt atgcgtggaa aaagagtaag
    42601 ccatccattg ataaactaaa tgcagttgct gactatttcc atgtttcaac agattatttg
    42661 ctaggacgca cagatgatcc taacgcggga gttgcaccag aggaaagaaa actaaccgtg
    42721 gaagaagctt tagcatctgt tatgagtagc gacggaaaac cgctcaccga taatgatagg
    42781 gaaatactat cagctatgat tgaagcatat ttagagaaaa aagataacta aataagtagg
    42841 tgagtcattt ggacagtcaa attgaaatga taattaatga actcggcgtt aaggtagaag
    42901 agcgtgaaaa ccttgatgcc gatggccatt atgttgcttg tatgaatacc atagtaataa
    42961 aagctaattt atctaagtat agaagacaaa gaaccttatt acatgaatta ggacacgctt
    43021 ctaaacatca tgataattat tttttatata acttagcatt ctctctccat tcaaaaatgg
    43081 aatatgaggc tgatcgcttc atgattgaaa aattattaga tagatatatt gcaaagtctg
    43141 aattagaacc acacaatatc aattacatga aatttataga agataacaat ttaagcgttc
    43201 gcttcgaacc acttgtgaaa gaattattaa aagctcgcat ctattgttat gcagctctct
    43261 aaaatttttt aagcaaaaaa agaacatatg ttcaaaaata gaaaggtgaa caaaaatgat
    43321 atatacagaa ttcaaagaat ggttagaaaa aaacacaacc ggatacgaaa catttatcgt
    43381 caaagctact aattatcaaa ttg
//
LOCUS       DACEDO010000015        42935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEDO010000015 DACEDO010000000
VERSION     DACEDO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.015"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            248..532
                     /locus_tag="IWO52_000915"
     CDS             248..532
                     /locus_tag="IWO52_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0355197.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(813..1232)
                     /locus_tag="IWO52_000916"
     CDS             complement(813..1232)
                     /locus_tag="IWO52_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0355198.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1234..1818)
                     /locus_tag="IWO52_000917"
     CDS             complement(1234..1818)
                     /locus_tag="IWO52_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0355199.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1865..3340)
                     /locus_tag="IWO52_000918"
     CDS             complement(1865..3340)
                     /locus_tag="IWO52_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0355200.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3521..3832
                     /locus_tag="IWO52_000919"
     CDS             3521..3832
                     /locus_tag="IWO52_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355201.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3947..6283)
                     /locus_tag="IWO52_000920"
     CDS             complement(3947..6283)
                     /locus_tag="IWO52_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0355202.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6425..7093
                     /locus_tag="IWO52_000921"
     CDS             6425..7093
                     /locus_tag="IWO52_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0355203.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7096..8139
                     /locus_tag="IWO52_000922"
     CDS             7096..8139
                     /locus_tag="IWO52_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0355204.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8376..9257)
                     /locus_tag="IWO52_000923"
     CDS             complement(8376..9257)
                     /locus_tag="IWO52_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0355205.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9416..10144
                     /locus_tag="IWO52_000924"
     CDS             9416..10144
                     /locus_tag="IWO52_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0355206.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10300..10617
                     /locus_tag="IWO52_000925"
     CDS             10300..10617
                     /locus_tag="IWO52_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0355207.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10610..11218
                     /locus_tag="IWO52_000926"
     CDS             10610..11218
                     /locus_tag="IWO52_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0355208.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11234..12064
                     /locus_tag="IWO52_000927"
     CDS             11234..12064
                     /locus_tag="IWO52_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0355209.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12202..12690
                     /locus_tag="IWO52_000928"
     CDS             12202..12690
                     /locus_tag="IWO52_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0355210.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12741..13613
                     /locus_tag="IWO52_000929"
     CDS             12741..13613
                     /locus_tag="IWO52_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0355211.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13789..14151
                     /locus_tag="IWO52_000930"
     CDS             13789..14151
                     /locus_tag="IWO52_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0355212.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14160..14912
                     /locus_tag="IWO52_000931"
     CDS             14160..14912
                     /locus_tag="IWO52_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355213.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14926..16728
                     /locus_tag="IWO52_000932"
     CDS             14926..16728
                     /locus_tag="IWO52_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355214.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            17005..18045
                     /locus_tag="IWO52_000933"
     CDS             17005..18045
                     /locus_tag="IWO52_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0355215.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18097..20241)
                     /locus_tag="IWO52_000934"
     CDS             complement(18097..20241)
                     /locus_tag="IWO52_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0355216.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20598..21971)
                     /locus_tag="IWO52_000935"
     CDS             complement(20598..21971)
                     /locus_tag="IWO52_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0355217.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21971..22366)
                     /gene="rbsD"
                     /locus_tag="IWO52_000936"
     CDS             complement(21971..22366)
                     /gene="rbsD"
                     /locus_tag="IWO52_000936"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0355218.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22394..23311)
                     /gene="rbsK"
                     /locus_tag="IWO52_000937"
     CDS             complement(22394..23311)
                     /gene="rbsK"
                     /locus_tag="IWO52_000937"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0355219.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23419..24432)
                     /locus_tag="IWO52_000938"
     CDS             complement(23419..24432)
                     /locus_tag="IWO52_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0355220.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24513..25112)
                     /locus_tag="IWO52_000939"
     CDS             complement(24513..25112)
                     /locus_tag="IWO52_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0355221.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25298..25999
                     /locus_tag="IWO52_000940"
     CDS             25298..25999
                     /locus_tag="IWO52_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355222.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            26009..28720
                     /locus_tag="IWO52_000941"
     CDS             26009..28720
                     /locus_tag="IWO52_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0355223.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29171..30799
                     /gene="treC"
                     /locus_tag="IWO52_000942"
     CDS             29171..30799
                     /gene="treC"
                     /locus_tag="IWO52_000942"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0355224.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30859..32040)
                     /locus_tag="IWO52_000943"
     CDS             complement(30859..32040)
                     /locus_tag="IWO52_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0355225.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32292..33815
                     /locus_tag="IWO52_000944"
     CDS             32292..33815
                     /locus_tag="IWO52_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0355226.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33884..34612)
                     /locus_tag="IWO52_000945"
     CDS             complement(33884..34612)
                     /locus_tag="IWO52_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0355227.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34625..35905)
                     /locus_tag="IWO52_000946"
     CDS             complement(34625..35905)
                     /locus_tag="IWO52_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0355228.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36046..36648)
                     /locus_tag="IWO52_000947"
     CDS             complement(36046..36648)
                     /locus_tag="IWO52_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0355229.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36893..37099
                     /locus_tag="IWO52_000948"
     CDS             36893..37099
                     /locus_tag="IWO52_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355230.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37411..38130
                     /locus_tag="IWO52_000949"
     CDS             37411..38130
                     /locus_tag="IWO52_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0355231.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38211..38894)
                     /locus_tag="IWO52_000950"
     CDS             complement(38211..38894)
                     /locus_tag="IWO52_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0355232.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39112..41298
                     /locus_tag="IWO52_000951"
     CDS             39112..41298
                     /locus_tag="IWO52_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0355233.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41644..42054
                     /locus_tag="IWO52_000952"
     CDS             41644..42054
                     /locus_tag="IWO52_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0355234.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            42068..42424
                     /locus_tag="IWO52_000953"
     CDS             42068..42424
                     /locus_tag="IWO52_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0355235.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        13994 a         7551 c         8584 g        12806 t
ORIGIN      
        1 agtgccgtag atgcaagctt ttgaacaccg tttaaggaag aggttgtgag cctgccgttt
       61 aactctgagt cacaaggagc aatttcaaaa aacagcttct catgtttgtt gactattgtg
      121 acttgtgacc gaggagttgg caggagaaca ccgtttattc gaaaaaagga actgcgacaa
      181 gacttttgtc acagttcctt cttttttttg ctatgctata ctagatgtaa aaaataggag
      241 aaataaaatg agggaaattt attcttttga atcaaaaatc tatgcttccg aagtcggcaa
      301 gggcggggca tatattattt ttccgtatga tataagaaaa gaattcggtg ctggaagagt
      361 taaagtccaa gcaacttttg acaatgtgcc atatgaaggc agtatcgtga atatgggcgt
      421 gaaaaatcca gatggttcag tctgctacat ccttggacta cgaaaagata ttcgtaaaaa
      481 catcggaaaa gaaatcggtg atattgtagc agttacagtg aagcaaaaat aaaagaggtt
      541 gtgacaaaag acgtgtcaca acctcttgat aaacggtgtt caaaagccag tttctcggta
      601 gtaagtcaaa tttatgcaaa aatgtggaaa cccatgttta caaaattttt cttactactc
      661 gaaacgaagc ggctttatca caacctcttg ataaacggcg ttcaatcagc tgtccctcgg
      721 tattagctca aaaacggcaa aatatgagaa gctatttttc catttttttc tctcatactc
      781 aggtcataac gctgatttca caacctcttc tttcataatg gtttttcacc atctttataa
      841 taaactggtt ctttgatatg gaaatctatt ttttccaacc aatctaaaat cttgtaacct
      901 aactcttctg acatcggaaa agaggcgttg atttgtgcat tggagatatg gtcaacatgg
      961 ataccggaag tgatcgtaca acttcccgga acggcagttt tgatgatcac agctattttt
     1021 tcggctaaga catcgtcttt atggaaccga ccatcgtggc tagggaaacg tacagtttgc
     1081 atctctgtat cacgagaaaa tgtagtgact gttccgatat gtggtgtatc tccgcctgtt
     1141 aaagtgatca ataagtcttt accgattact ttaacagcag cttggatcgt gaaacctagt
     1201 ttggttactt caactatcgt ttctttttcc atattaatca ccctgccaac gttttgaatg
     1261 ctcatttaca atttcaaatt gatccaaaag tttcattgta tggtgattaa caagatcttg
     1321 gatcgtttga ggctgattat aaaaagcagg aattggtggt atgatttgca cacctagtcg
     1381 tgctaattta gtcaagtttt ccaagtgaat ggcatttaat ggcgtttcac gaggaacaat
     1441 gactaacttt cgttgctcct tcattgttac atcagcagca cgtgcaatca ggttgtcgcc
     1501 taaaccaaca gagatacctg cgactgtttt catacttgcc ggtacgatga tcattccatc
     1561 ggtcaaaaat gatccgctgg cgacagcagc acctaaatct ttgtttgaat aatggtaatc
     1621 aaacaggctt tcgaattcag ttagtgataa gtcggattcc agctttaagt tctgttttgc
     1681 ccatggactg atgattccgt gtgtttctat catcggatat tcttttagtt tcttcactag
     1741 gttgatggca taaatggtgc ctgaagcgcc acttacacca atcacgattt ttttcttccg
     1801 ttgtgccaat tgttccattt tccgcactcc tttttatgat gtcaaaacag ttgcagatca
     1861 ttcattattt caaatatttt tcccaatctt ctacttcttg gaattttgca cgtacaaaat
     1921 gttctttcat atcgaaagga actgtaccgt caaagatagt ttttgcactt atcccacgga
     1981 aacgaataga gccaggatta tattctggta attctgatgg gtccaatgga tggttcctca
     2041 ttcctgggat cacgatgata tctttatctc cttggaaacg agtgttgaaa gtccaaacta
     2101 catcgttcat gtcgaaaata tcaacatctt catcgactaa aatgactgtc ttcaattctt
     2161 tgaaggctga taaagcaaga atagctgctt gacgctgaat accttcatcc gcttcgtttt
     2221 ctttacggat ttgcatgatg gtcatcaatt taccaccacc tgcaggagga ttgtagacat
     2281 ttactacttt tcctggaata gctttatcga ccaaggcaag aatacttgct tctgtaggaa
     2341 taccggccat agatacgtgt tcttcactgg gaccaatcgt tgtttgcatg attggattgt
     2401 cttttcggtg agtaactgct ttgactttca ctacttgtac agctggatta gcatccccag
     2461 tatagccagg aaattctggc attgcttttc ctgagttcgt gttgatatct tcttgcattg
     2521 tttcatttgg catgatttct gcttcaatga tgaattcagc acgagcaata cccacttcat
     2581 cgactgctat accatcaacg atttgaatag gttcgttacg caaagctcct gcacaccata
     2641 gttcattgta gccaagtgga gtggtaggtg gttcgaaagt cgtgccaatg gcaattgctg
     2701 gatctaaacc aatattgata gtgataggca ttggtttatt caaggcttcg aattgttttt
     2761 ggaagtgacc aatgtggcgt ccgccaggca tgatgtacat accgatcgta tctttatctt
     2821 ctagaaccat acgatggatc gttacatcgc tcattgtttt ttctggatct gatcctaaga
     2881 ctacacccat tgtgatgtat ggaccagcat cttcaggagt atttgtaggt gcaggcaaaa
     2941 tgttgcgaat atcgaagtct gcatcggtag ctagatgaac gacttcttgt gctggagcgt
     3001 ctgctttgtc tactttcaca ggtttgattg gattttctac agcgtctttc aagaaacgac
     3061 caagtgttcg ataatcatgg tgcaaaattt cacctacacg tttacgacta gccattgttc
     3121 cgatgttcac acgtgtgccg gggaaacctt tgatattgtt gaaagtcata gttggtcctt
     3181 cttgagtcgg acgttttact gtaccgccag caccgatata acggtaaaca ccagataatt
     3241 ctgcttcagg atcgatttca atatctgttt cgtgatattg acctggaagt gtttttagtt
     3301 cttcgatgac ttttcttaaa tcatacggtt gatctgtcat gagagattcc tccttagttg
     3361 tctttaccta tctatagtaa aaggtgaaaa agaggaaaac aattcaactt gttcggtaaa
     3421 gtattctaca atggaatgga tgcgtttttt tgataataga ataaactatg atatacttat
     3481 cactaaaaga aagcgcatac ttagtgttgg aggaaggaaa gtgaaaaaaa tggaggagtc
     3541 atttcctaaa gcagtaaaag tggaaaatat agcgaatatt ttgaaagtta catttgaaaa
     3601 cggagaagta aaatacgtga aatctcattg gacagaagag ataaccgatg ccttgcaatt
     3661 tggaaaaaaa ggaagaggaa aaagaaaaaa tcttctcgca ctttctcgta atatgtggat
     3721 tgggactgaa gtaacgattg aagcagatgg aacagtattc ataaatggaa aagacaggta
     3781 tacgccagaa gagttgtggt acaaaggaaa gaaaagtata ccagaattat aaaaaataat
     3841 taaaaaaaac agcgcgaaac aaggaacata ttacttcctc gtttcttgct gttttctact
     3901 atttcagcgc tcttatgact caaatctaaa tgtggttgtc acaaccttat tctgcacgca
     3961 aagcgatagc ggcgtctttt ttcgctgcca ttcgtgcagg aagatgaccg ccaagcatcg
     4021 tcaatatagt agagacgatc actagaataa tcgcatggac aggatttagt tgagcaacat
     4081 tttttagatc cgtcatgttg tataagatgc tattgatagg gaacgtagct agccatgcaa
     4141 taaacacacc taatatccca gaagaaatac ctaaaataca cgtttctgcg tcaaatacgc
     4201 gagtgatgtc ttttttacgt gcacctaaag cttttagtac accgatttct tttgtccgtt
     4261 caatcacaga tgtataagta atgatactga tcatgatcat acttgttact aaagagatcc
     4321 ctgcaaaagc aataaggaca taagtgatag catccatcaa tccgccagtc agttcagtca
     4381 tcgttccagc taaatcagta taaatgattt tatcttcatc tgatttgcct ttattgtaat
     4441 catctagata atccaagatc ttttctttgt cttcaaaatt gttcggataa atcatgatac
     4501 ttgaaggcaa gctgtctcca cctaaataac taagcaatgt ttgtttggca ttttcgtcaa
     4561 ctttttcagt agttaagaca tttacaccac tgtctctttg agctttgacg atttctgatt
     4621 ctttgttttc gttgacgatt tgagtcgtta attgatcgct ataagcgatt cctggagata
     4681 agagattcat tgtagaagaa gatttcacgc gtaggatccc cgagatcttc aattcatcag
     4741 aagcattctg atacatcgca tcataatctg tattagggat aaaattccca gttggaagtt
     4801 ttgtatagta agtattgttg tttactaatt tgaatgttgt accaacgata tcatcaaaat
     4861 caagtttttc gtcttctttc acatcaaatc caagattctt caatgcgttg atattagtat
     4921 tattattgcc gtctacgatc agcacgacat ctgttgctga ggctggataa cttccggcaa
     4981 gtaaagaata attgtctttt aaaaagtttt ctttgctagt gtcaagctgt gtagggaaac
     5041 ttgacactcc cacacctgtc attgctgaca tcgtactaga taaagaaaga gattccgtat
     5101 caggattttg attcgagaag ctgacaggct gtactttgcc atttacatca cgtaaaagat
     5161 tgattcctgt agtacgggta aatccgatat tattacttaa gttaggatcg atgtctgtta
     5221 cgtaatcgac gtatgtttga tcaatcttat tcgtgtgctg tgctttatct tcttcgctga
     5281 cttttgcagt aatcgttttg ctgtctggat aatccgcttt gcttgcaccc aatccggcat
     5341 cgtcacgagt ctggttagtc gttaccttag aaatagtaat cggaaatttc gccatcgttt
     5401 cagcttgcgt gttatcgatt tgtttttgga atccagaaga aagggacaga acgatcgcga
     5461 ttccgatgat cccaatactg gaagcaaagg aggtcaagaa agtacgtcct ttttttgttc
     5521 ggatattgtt gaaggaaagc tttaacgctg tccaaaaact catctttgtt ctgcgtaagt
     5581 taaattgatc atcttttggt cgttcgatgt gaggatggga atctgttaag atcttaccat
     5641 cagaaaattc aatgattcga tctgcgtatt gatgggccag ttcaggatta tgtgtgacca
     5701 tgatcactaa tttttctttc gataactctt ggatcaattc catgatctgg atacttgttt
     5761 ctgtatctaa cgctccggtt ggttcatcgc acaaaaggat atctgggtca ttggctagtg
     5821 ctcgagcgat ggccactcgc tgcatttgtc caccagataa ctgatttggc tttttatgca
     5881 tatgatttgt caagccgact cggtgcaatg catcttctgc ccgttttcgt ttttcgtctt
     5941 tagaaacacc acttaatgtc atcccaagtt caacgttttc aataatgcct agatgaccaa
     6001 tcaagttgta actttgaaaa atgaaaccga tagagttgtt tcggtaggca tcccagtcac
     6061 tgtccttaaa gtctttagta gactttccgt tgattaccat atcaccggaa tcataattat
     6121 ctagtccacc aatcacattc agcatagtcg ttttaccaga accactagga cctaaaatcg
     6181 ctacgaattc tttttgtctg aaagcgacag acacaccatc caatgctttg gtggtggttt
     6241 cgccgacttt ataatatttt tttatatctt ttaattgaag cataatcacc cttctcccgt
     6301 attgaaatat tcggtatact aataagtata tcagaacaat gatcgtagta tacgaaataa
     6361 atgttaactg aagatgaact acaagaaaaa atctagaaat ttttcaaaaa acagagggag
     6421 atcaatgaat cgtattttag tagtagaaga tgatccgcaa ttacagttgc tttaccgttc
     6481 tgtgttggaa cgagcgggct tttcagtcat caccgcattg aatggagagg atgcgctgaa
     6541 aaaactagaa gcgacacgta tagaattaat cattacagat atcatgatgc cgaaaatgga
     6601 tggctatgac ttgctagact cattacgtag caccagattt gacacacctg ttctaatgat
     6661 tacggcaaag gccgattttg aggataagaa acgcgggttc aaattaggag cagatgatta
     6721 tatgacaaaa cccatcgatg tcaatgagat gatcctacgt gtggaagcat tgcttcgacg
     6781 ttcaaagatc aattacagca gtagcttgat aatcggacaa acaaaattag agctagatgc
     6841 ttatacagtg ttacaagatc agcattcgtt gattctgcct caaaaagaat ttctgcttct
     6901 gtataaatta ctgtcttatc ccaacaagat cttcactcgc caacagttga tggatgatat
     6961 ttggggatta gatacgaata cagaagaacg aacgatcgat gtacatatca aacgtttaag
     7021 gacccgtttt gaaaagacgg aagattttca aatcatcaca gtacggggac taggctataa
     7081 ggcggttatt taaagatgaa aaaaaagcta ttttcacaat tatggatcta ttttgctagt
     7141 attgtcttca tttcgatctt agtgacactg ctgtgttttc tcggttttat tttcttattg
     7201 tctcgacatt ctatctcgcc tgctgaatcg gctggaaaac caacgttata cccgttattc
     7261 gtctttgcgg ggttcagtat ggtcgtgggt acaggaatat cgatttttgt tgggcggcgt
     7321 atcctgcatc caatcagtgc gctagggaca aatatgagtc tggtagcaac gggagatttt
     7381 tcgattcgaa tggatgaaca gcagaaagta gcagaagtac agcaactgta taaagatttc
     7441 aacgttatgg tccaagaact caatagtatc gagacactga ggaatgactt tgtgtccagt
     7501 gtatcccatg aattcaaaac accactagca acaatccaag gatatgttca attgttacaa
     7561 gccccaaatc tctctgacga agaacgccaa atatttttat atcggattat cgaaagtatc
     7621 acgcagcttt cacaattaac agagaacatc ttaaaattga acaaactgga aaaccagcgt
     7681 atccaactgg aaaagaaaga gtatcgatta gatgaacaaa tcagagaagt aatcgttttt
     7741 tttcagccta aatgggagaa agaacagcta gaattggata tcgaacttgc tgcagtaaat
     7801 tacacaggca atgaagagtt tttgtaccaa gtgtggctga atatcatgga taacgccata
     7861 aagtacaatc aaatcaatgg acagattcat atcaaactat ttgagacagc cacagagatt
     7921 gtactggaag tgaccgattc aggtgtagga atgaatgaag agaccagaga tcgaatgttt
     7981 gaaaaatttt atcaaggaga tacaagtcgt caaatttctg ggaatgggct aggactttca
     8041 ctggttaaaa aaattctaga actgcatgat ggacgtattg attatagcag tatcgaagga
     8101 gtaggaacga cagcaatgat acggttaagt aaacaatagg aaaaagaata aaacccctaa
     8161 aaattctatt gaaggttttt aggggttttt agtcgttttt ttgtgattag ggaaatgttc
     8221 ttacggttct tttggcataa aaatcgctgc tttcttattg aactacgaga aatgttagag
     8281 gtgtcattga aacaaggaca tgatgcaaga aagagagcga aagtcatgcg tcgctctctt
     8341 tttttgggtg gatagatgga cagaaaatgt agggcttagt tttcttcttg atttaaatac
     8401 tcccaaaatg tatctgaagc caaagaatgc tcaccgactt tacgagtaaa tgcaagataa
     8461 ctctctgttg ttggagaaat agggagaatc acagtatttt cttttaatag attcttgact
     8521 gttttttcca taacaatagc aatccccatg tcatttgcaa tcatattgat gatcaagtcg
     8581 attcttgctc ctttataaga gatttttggt tcaaatccag cttcgcgaca cagatctagt
     8641 acaggttgat acaagttcgt agtggggcca aggagcaaaa agttctcctt tttcagttcg
     8701 cttagatcca atttcttttt actagctaaa ggatgatttt taggtagaac tgctacaaaa
     8761 tcgtctgttt cagtaggcaa ccactcaatc gaacttggtg gggcctggaa acttctagca
     8821 aagtggatcg tgtcactatg ttcattggcg tcattgtaag ttaattcgac actttccact
     8881 tctttcaact ggactgtaaa ttcaggatgg cgtttcaaaa actcggtgat tttggaaaaa
     8941 cctttataat tgatcattgt cggtatcgtt aagatattta atgaaagatt gttgtctttc
     9001 gtataggttt caagcacttc ttgcatagca tggtattgtt ctacgacatt ttttacatat
     9061 gggagcaata agcttccagc ttcggtcaat tcgatttttc tatgggcgcg ttcgaataat
     9121 ttgacgtcta attctgtttc taacgaaaga atctgtttag aaatatttcc ttgggtggtg
     9181 aattgtcgat cagcagtttt ggtgaaattc attgtttcgg ctaaatcaag aaaaacttcg
     9241 aatttatgga tatccatcat attcctccta cttattccaa taaagaatac tttaacacaa
     9301 aaaatgaatt ggtgtaatct tctctgtctg ttacaatctt tcttgcagcg aaagcaataa
     9361 ttacagaaat ttttctgatt tttttcgctt cgtagaaaaa gagaggagtt ttttgatgcc
     9421 aactggagtt ttgatcaatg taggttccgt tttgtttggc ggactaattg gcggattagt
     9481 aggaaataaa ttaagtgaac attttaaagc acagctgacg atggtttttg gtgtgtgttc
     9541 gatggggatg ggaatctatt ccattgcgcc gatgaaaaat atgccggctg ttatttttgc
     9601 tttagtgata ggaacagcga ttggtttgat tgttcattta ggtaatggga taaataaggg
     9661 ggctgcgctg atgcaagtac ctatttctaa aatcttccct agtgaaaagc ttggtatgac
     9721 ccacgatgaa tttatcagca cattagtgac ggtcattgtt ctattttgtg caagtggtac
     9781 ggggatctat ggaagtttag attctgggat gacaggagac agtacgattt tgatttctaa
     9841 atctgtcctt gattttttca cagcagctat ttttgcgtgt aatttagggt atgtcgtttc
     9901 tgtgatagca attccccaat tcattatttt ttatattttg ttcttattgg caaaatttat
     9961 ttatccattg actacaccag acatgatctt agattttaaa gcttgcggcg gattcttgat
    10021 ggtcgctacc ggttttagaa tgatcaacgt gaaaatgttc ccagtagctg acatgatacc
    10081 tgcaatgatc gttatcatgc cactgagctg gatgtggaca aattggatta tgccactgtt
    10141 ataataaaaa tcaaagagaa gccatttttt gctgactaaa aaggatcaaa tcttttagtc
    10201 agtttttttg tatttgacaa actgattttg aaactatatt atatgttgta taatatagat
    10261 tgaaataaaa tattacactt gagaatagaa ggtgacgtaa tgcacatcca gattccaact
    10321 gttcttcttg atggtactgt gctagcagca ttaagcaagg aagatatgta tgggtatgcc
    10381 ttaacaaaaa tcgtacaaga aagtctctct gtcagtgaat ctacactata tccggttttg
    10441 agaagattga aaaaagaagg atatcttgag acctatgatg aaccttttga aggcagaaac
    10501 cgtcgatatt actcgttgac accaatggga agtcgtcatt tagaagaaat tattgatgaa
    10561 tggattgttt ttcgagattc tttagaccgg ttattggagg agagcacaaa tgaatgaata
    10621 tttgaatgaa gttgtggatc atcttcaaga aattcctgaa gacgatcgtt ttgatttgat
    10681 ccagtattat gaggaatatt ttttagattc tggaaaaaca ttagatcaga tcattgatga
    10741 atatggtaca ccaaagcagt ttgccttaaa attaaaaatc agttactttt cagaagaaga
    10801 acaagcagaa aacgagttag tgaaaaatcc aaaaagacaa attcgcctga tttggttaat
    10861 cattatcggt ttatttgctt ctcctgtcct cattccacta gcactggggt ttatattggt
    10921 tattggggga ctattggttg ctttactagc gctcattttt agtatctatg ttttatgtat
    10981 cagtatctta ggtgttggcc tattcatgat catctcagga atcagtgtgt ttggacaatc
    11041 gattctgagt ggattattct ttatcggtgt cggtctgttc gccacaggaa gcgcagtatt
    11101 tatttcgcca ctgttgctga aaggaacaaa agggcttttt tatgtcatga tgaactttgt
    11161 taaatggatg ggacgacgtt ttattacaaa aagagatctg cattcagcgg gcgtttgaag
    11221 gaggacacat aaattgagat tgaaacattt tttgattacc ggtattggtc tgatgcttac
    11281 tggtggcctc ctagcaggtg caacttatac attaggcgct caaaagagtc tgacttggga
    11341 taatggtcct cggttgatcg atatggttac tgagacaaaa aaaatcaagg atgcaaacaa
    11401 aatcgtcatc catgcagaaa atcaaacagt ttcggtcata cgcggattgg attttgagat
    11461 aaaaacgact tatgataaga cagaaaagcc agaaataaag gagaaaaatg gcgtgttgac
    11521 tgtgaatgca gtcaaaaaat caacaggtgc aatcattagt ttagaagaca caagtgcaca
    11581 aatcactatt gttgtccctg cagatcatga attatctgaa ttgacaatcg aagggaataa
    11641 tagtgcaatt gatatcaacg aagtatcaat caaaaaagtg aatactaaga cacatgatgg
    11701 ctgggtatcg ttcaatgaat ttacaggagg aacaataact gcgaaagatg aattaggctc
    11761 acttcgatta gatgaggcca atgtggataa cttgatcgtc acaggtgaaa acagcaactt
    11821 ccatctagaa gacagtttga tcagcaaaga aggaaaagtc atcctaaacg gtggcaattt
    11881 atctttgcat gagatggaaa acagtggcta tactatccaa acgtctggtg aatcttatat
    11941 tgaaaaagac taccagccaa taacaaatac tttgttagaa caaggacaga aaaagctaaa
    12001 gatcactgct aaagacacat cgatcaatat tacgacggat caagaggaca atgaaacaga
    12061 ttgatcctgt actcaactgg atattttaag aaaaaacagc aaagatcctt ccaatctttg
    12121 ctgtttttca tcgcttaacg cacataacta tagtaaaata agaaagaaaa tatctaaaaa
    12181 gaaaacaggg tgaaagagta tatgaaggta gaagaaggtc aaagagaaaa gttaaaaaca
    12241 gaaatggaaa gattacatac ttatatcact caactaagcc aaaccttcta tgatccagac
    12301 aaagaaaaag tgatggttaa ttacccgaac aattcagaag gtcgccaatt agagcaagtg
    12361 taccatgaag ttttcaaaca tttattgacg gtgaagaaag agctggatta ttattcttta
    12421 ccgattatcg atacaggaat attgaagtat gatgggaaga aggaacgatt tatctttaaa
    12481 tctgttcggg aagatttgcc tctttcagca gggatggatc tagaggtctt ggtagaagac
    12541 tattttacag aagaaaagca ctgggttcga acaaaattgg attatttacc gcaggcagcc
    12601 ggagggacac aggcaagcgg ttggtacatc actgaagaca aagagttaga actagaaggt
    12661 gtaatggcgc gtattcgaaa aaaaaattaa aaaagtaaca ttagatattt attgaaggat
    12721 aaaggagcgc aggaagtagc atgagagaaa aaagagcatt ttatatcaat ggttatgcag
    12781 ggattgtcgg attggtcgta ttagctttag ttggattgtt tttcttctat ttaggtatgt
    12841 ggcaagcgaa agtttgggca ctttttttaa gtatttttct ttggttgatc acgttattgt
    12901 tgttaagttc ggcaactgta gtcagtccta atcaagcaaa agtaattttg ttttttggac
    12961 aatatttagg tacgattcga gaaaatggct ttttccttac gatacctttg gcacaaaaaa
    13021 tgactgtttc gttgaaagtt cggaatttca atagttctgt tttaaaagtc aatgatttgg
    13081 atggaaaccc catcgaaatc tcagcagtag tagtctttaa agtcgttgat acagcaaagg
    13141 cattattcga tgtagcgtat tatcaagatt ttgtagaaat ccaaagtgag acagccattc
    13201 ggcatatcgc tagccaatac ccgtatgata cattcaatga gaatgatttg acgcttcgag
    13261 gaaacacgac ggcagtgtct gacgagttgc aaaaagagct gcaagagcgt ttagcagtag
    13321 caggtgtcga agtaatcgaa acacgcttga accatttagc ctatgcaaca gaaatcgcta
    13381 gtgccatgct ccaacgtcaa caagcaaaag ccattttgtc tgctcgacaa accattgttg
    13441 aaggagctgt cacaatcact caaatggcct tagagcaaat cgaagatgga caagaaatca
    13501 atttcaccga tgatcggaaa gtccaattga tcaataattt acttgtctca atcattacag
    13561 acaaaggaac gcaacctgtt atcaatacag gcgacatgaa agaccgtaag tgacatactt
    13621 tttgtaagat aggaagaagg agtttaggac ggattattgt cttgaactct ttttttagtt
    13681 gttttaaaga aaatgacaaa attgaaagaa gaatgatagt tgattcgaag gcagaattgt
    13741 tcgctttttt ctataatcag tatataaagt aagagagaag gggaaaaagt gaaaaaactt
    13801 ctgatcggtc tacttgtttt catcgttgtg gtacttgcag gggtgaaaat cgcagattac
    13861 gtagttatgg gtggagaaga atactatgta caaattacga cagatggtac aaaaacagtt
    13921 tcaaaatcag actctggaca agaaacagtc aactatagat atatcctgcc tggctatacg
    13981 aaagagggta aggaaaaaga actggacttc aagggacaat tgactcgacc gcttcgtaaa
    14041 ggagcatatt taaaaatcac ctggaacaaa aataaaggtg tcacttcgta tgaagaagtc
    14101 gaaaaaaaag acgtgccgaa aacagcactg gaaaaactaa ttaaagggta gggaaagaaa
    14161 tgaacaaaat agtcgaagta cagaatgtaa caaaagtata cggaaaaaac aatgagaaaa
    14221 agacgcaagc attaagcggc atcagcttcg atgtagaaaa aggcgaattt attgggatca
    14281 tgggcgcttc tggttctggt aagtcgacac ttttgaatat tttatccaca ttagataaac
    14341 caacagatgg gcatatccga atcaatcaaa aagatgtgac cacattaaaa ggaaatcaac
    14401 tagcagattt tcgtgcgaat gagattggct ttattttcca agatttcaat ttattagaaa
    14461 gcttgacagc acaagaaaat atcgctgttc ctctttcttt acaaggcgtc agaccaaaag
    14521 aaatcaagca acgtgtccaa aaaattgcag aacgtttatc catctctcat attttggaaa
    14581 gctatccttc tgagatttcc ggtggacaaa aacaacgggt cgccgccgca cgtgcattga
    14641 tcacacaacc aacgattcta ttaggggatg agcctacagg tgcgctggat tccaaaagtg
    14701 cccgggatct tttagatacg atggacgaat tgaatacaaa agatcatgta tcgattcttc
    14761 ttgtgacgca tgatccgttt tctgcaagtt attgccaacg tattttattc atcaaggatg
    14821 gcggtatcca tcaagaagtg aaacgtggag atcaaagccg tgattccttc tatcgagaaa
    14881 tactatctat tcttggaaac ttggaacagt agggggagat tgactatgct ttggaaatta
    14941 tcacttactg ggatcaagag ccgtttaaga gactacttgg tgttattctc aggattagtt
    15001 atggcatctg ctatttttta catgtttgaa tcaatggcaa gcaacgaagc ttttttgaaa
    15061 agtaatacga tcgttgcggc tacagttatc attttccgat tcggttctgt tttactaggg
    15121 atcatcacat tcgtttatat tttatatgca aattcctttt tgatgacgat gcggcaaaaa
    15181 gactatgcaa tgtttatgat gctgggagca aaaggcagaa aaatcgcaca gatgattttt
    15241 tctgaaacat tcttagtagg gatcgcagct actttagtgg gttcagctgt cggtgtagga
    15301 ttgacaagta tcgtccaccg attattagtg gatcagttag atctcaatat ttcgcatttt
    15361 acacctttta gtattcacgg gttgttgatc actgttttgt tttttgctgt tttgttttta
    15421 ttagctgctg ttgtcaatgc tttttcgatt gtcaaaaaac cgattttagc attgattcgt
    15481 gcggatcaga caccaacaag aatcaagcag cataaattct tgtttttcct tgaagtagta
    15541 ctgggaattg cttttctagg ctgcggttac tatatgatga aacacgtggg gacctatcaa
    15601 gtctttggta taggggtagc cttagttacg atcgtattag gaacttattt cattttccat
    15661 tctgtgatca ttttcttttt atcattacta aagaaatcag aacagatttc attgaaaaaa
    15721 ttgaacaatt tcacattgtc acagttaaac tttcgtattc gtgaatatac acaaatgctc
    15781 tcaatggtcg ctatgctgtt tgccttggca ctgggcgctt tgacggtagg attaggtttt
    15841 aaaaatgaaa ttgcagactt gacagaaaac aattctggat atgatctagt gatgaataat
    15901 cctcgggcag aagatcagca aaaaatcaaa gaactttcac caacactttc tgcttcttat
    15961 actcaaaaag aagatgcggg aacgatttac tataatgcaa gtgaatttgc ggctcaacca
    16021 ttgatcatga taaaacatga agatggaaca ccgccgaaga tcacgaaaga aaaagttcct
    16081 gctaaaaaaa tgaatgaact gactgtacaa gaggaacttc gaagctatga attgccggaa
    16141 cagcaagaaa aagaaatcaa ggtagtcagt caatcagaat ttgatcaact gaacttgcca
    16201 caatccactt tacagctagt tcaggtaaaa gacttccaag caaacctgaa accaatcgaa
    16261 acacttgttg ctcaaaataa aatgaacaat ccatctttgc aaaaagtaga gtacagcaac
    16321 cagaaatatg aaatgtatga agtgtataat ggtctattct ctggttttga atttatggga
    16381 ttcttcctag gtttagcttt cttgaccatg cttgctagct gtttgatgtt caagatcttg
    16441 tcaggaagca agagtgacat tgctcgttat gaaatgcttg aaaagatagg aacacgacgc
    16501 gggttgctta aacaatcgat ccgtagagaa gtaggcgtgt tattcttagc accaggaatc
    16561 ttaggtgtag tccatgtctt attcggatta caaatgtttg gtctattcat gcaaaatcca
    16621 tacaaagata tttgggttcc gtttacgata ttcttcgtat tgtatttcat ctactatgta
    16681 ctgacgacat ggttatatac tggaatcgta ttgaaaaaga gattgtgaaa aagctgtcct
    16741 gcatcaagta ataagaacag ccaagcaaaa atagctcctc atatttttcg tttggttggg
    16801 cttattaccg cagatgcaag cttttgaaca ccgtttgttc ggaaaaagag gttgtgacaa
    16861 tatttttgtc acaacctttt tttcacggtc ttttattaga atatagtaaa ttttttctcg
    16921 ttactcacgg aataagctgt taagaaaacg gtttccatgt atgctggaat cagtgcaaat
    16981 gaaagaaaag agggtgcaag gaacatggaa atcaatgaac aaaattttgg cgaaaactat
    17041 cacttgatta cgatcacgaa tcaaaatggg acatctttgt ctgtcagtga tcttggggca
    17101 cgggttgttc attttcaaac aataatcgat aatgaaaaac gagagttgat cttaggtttt
    17161 gatgaagcaa aagaatatct agaaaaagac ccttatatcg gtgcttcaat cggacgtatg
    17221 gctggacgga ttacgaatgg cacattcaaa ttagatgatg aaacgttcga agtaaaaaca
    17281 gacccggaca ctggtcattg tttacatggt ggtgcaccta gctttgaagc caagcagtgg
    17341 cattatacag tcatcaatgg tgaaaacgaa gcatcggtca ttttctatca gactagccca
    17401 gatatggaaa acggatttcc tggaacttta gatgtagaag tacggtatac gctgacgaac
    17461 gatgatacat ggcgagtgac ggtccaaggg atcagtgacc gcaaaacagt attcaatcct
    17521 acaaatcatg tctatttcaa tttaaccggt gatccagcac agtcaatcga cgaacatata
    17581 ctttggttga atagccgatt atttgctacg ctcaataaag atacaacgcc tacaggagaa
    17641 gtagtttcag taagtggcac agcatttgac ttccaacgtc ctaaaaaatt aggtgaggtc
    17701 tttgccagtg attttcgcca atcaatgata gtgggcggca tcgatcatcc atttttgttg
    17761 aaaaaacaat ctggtttact aaatgctgcg gaattgacaa gcccagatga aaaagtatcg
    17821 atccaagtaa aaacagatgc ttcaggaatc gtgattttta cagcaaattt tggagaagat
    17881 ggaccggaaa tgcgcgggaa taaattagta aatcatggcg gtatcacatt tgagactcaa
    17941 gaactgccag gagcggagcg acttgaaagt tttggagatg tcactctgga acctaaccaa
    18001 gtacgcgagt ttgtaacgga atacaaaata aatataaaga aatagaataa attaaaaagg
    18061 gagtgtgaca gtgtcacatc ccctttttaa tttattctat ttctttaaag gcagatcaag
    18121 ccagaatgat ttgtctaaac ttaacaaggt tagtacagga acagttgcag ctttaactgt
    18181 acgagtagtt gtttctggta tctgtcttgc cgtaaaattg ttccacaaat cccagattga
    18241 agcgacacgg aagatggaag aagcgttgtt gacgcgtttt atcgttgaaa attctaatcc
    18301 tgtgattggc acgccatctg ttgtccattt gattggcaca tcgaatcgct tagacatatt
    18361 gaggatttca gcgtttgctt cttcagttgt tgctgcttct tttttcggtt catcatattt
    18421 tgtcaggtcg tacgcttcga tgatcgcgtt caatttttgt gaatattggc gatctgtagc
    18481 ataacgtcct tccaaatatt tggttgcttc tttgtagtct tttgtttccg acttccaagt
    18541 tggtttgtag aaatcaggat tgccagtcag tccatttttg atcaagtcgg catagtcttc
    18601 caaactttcc ttgtaagaag gatatttacg gaattgtgcg cggatttggt agctttgtcc
    18661 ggaactatct tgttcaagtg tattgaaggt cacgctgctt cctttatatg atcctttgat
    18721 accaaaaagg ttatgatttg gttcgcttga taataagctg tttccagatc cactttcaag
    18781 gatagcttga gccatcatga ctgatgcgta gattccttct ttttgcccga tttcctgtgc
    18841 atcatctgca atctttttca agaactgatc agttgtttgg ttttgactga aatggaacga
    18901 gccatctaat ccatctaaaa tagatgagcc gcctgtcagc gaacctgaag cagcaggtaa
    18961 acctgctaag tttactcgga cagcaaaatc aggcgtgaaa tctgaaatag aagttactcg
    19021 aactttatct cctggttgag gcgcatggat aaattgtccg ttcccaatat agatacctac
    19081 atggtgagta acaccggaag aaggtcccca gaagacgagg tcgccaggtt gggcctgact
    19141 tactggaatc tgcgtacctg catattgctg ttcgcctgtc cagccaccga tataatgacc
    19201 ggttgctttc atatatacgt aataagtcaa accagaacag tcaaaagtat ttggaccttt
    19261 tgctccccat acatatggtt ttccaagatg cttgaatgct tctcgaacga ttgcttgttg
    19321 gacattcgtt gtatttgtag gcaatacagc atcaattggt gattttacaa caaaatcaga
    19381 agaagcagaa gatggattct ctataccgct ttgatttgct gtatttggac gagatgaagc
    19441 attgttcgtt ccgccattcg atgaaccatt gtttggatgg tttggttctc tctgagtatt
    19501 tccattgtta ttcgatccag atgggtttgt ttgattcgaa gaattcccgc tgttcgaatt
    19561 attgttatta ttgtttgaat tatttgaacc attattattg ttgttattac ccacatctgg
    19621 tttgtttggc gtttccggat ctggtgtact agggacttct tcactaggat cagttggttt
    19681 ctctgttgaa gtatccggtc cagctttaaa ttctgcagta atagataaat tgttcgtacc
    19741 tactgtaaag gtctttgcat cataatttac ttctttggca ccattaacag cgactgaagc
    19801 aatttgttca ccttcagctg gcgtaatagt gtaagttacc ttcgtttccg ctaataaacc
    19861 ttctaccgtt ccactttccg taatatccaa aggttgctgt tcaggggaat ccgctgtcga
    19921 agcagtcagt tggatcttac caatcaaaga aggagcaatt gtaagggaat atgtctcggc
    19981 tggcttttca ttttctgatg gatcagccgg atcaatttca gttggctcgg tcgatcctgt
    20041 tgttgagtta gttgttccag ttgggtcttc tgtaaaagaa tccgttgttt cactagtagc
    20101 tgagctggat gtagtcgtat ctgttgtgac tgtttgatca gctatttgat tatctacggc
    20161 aaatacggta ttggtcgaca aaacagacgg agcaatcagc caagtagcag ctagcaaacg
    20221 tatcgttttt ttatttttca taataaacta gtccttttcc tttcattgat tttctgtctt
    20281 attcagcatt cattagacat tttttggaac agataatcgt gtcgaaaagc tttttctttt
    20341 cataaatctg tctgtgtatt ctaaataaaa caatttgtaa ataagcaacg agcattcatt
    20401 cgtttttctt tgacgttcat tttattgcat agaccacctt tgtttgggga taaacaagtg
    20461 gggataaaaa tggttatgca tatgtaaatt ataccaaagt ttggaaagta tcacaaaaat
    20521 ttataaaaat aatgatccta aaaagaaaaa gactgaaata tgtgataaac atctcatact
    20581 tcagtctttt tcattaacta ttcataagac attaggtctt catcaaaccc gctaacgaga
    20641 gtagtgatca ttgctacgac tagactaaca gccagtcctg ctaagtacca gaaaaaagta
    20701 ccggaaatga atgcaggcaa tgtggtcaga ctaccaaata caaaagcgat cgctttcact
    20761 tgggcaaatc ccattacagc tccaccagca gctgcaccaa tgcaagcagc aacaaatggt
    20821 tttttcaatg ggatcgcaat tccgtaaaca gttggttcgg tcactcccgc aagtaaaatc
    20881 gaagcaaaag cagaaccgga gatcgaacgg gtttttgaac gtttacttcg cagaaataca
    20941 cctaaagctg caccagccat tccaaaattg gctgctcctg tcaacggaac gattcggtcg
    21001 aacccataag cagagatatt actgatcatg acagggttta cagcccattg gataccaaat
    21061 attaccaaaa tattccaacc accagcgatg attgcgccac tgatcggtcc gttaatatcc
    21121 atcatccaag taattgcggc agctaaacct tcgccaaggt aaacacccgc aggcccgaat
    21181 accattaacg tcaaaggaac cgtgataact aatgaaagta aaggtacaaa tactaattgc
    21241 atattttcgc gaatatattt tttgaggaat ttttcaacaa aagaataaaa gaaaatacct
    21301 aaaatcgccg gcatgacagt agacgtatac tgcatcagta ctacaggaat ccccagaaaa
    21361 tcagtagctg ctttgccact gtctgaaagt aaagaggtca gattcggttc aatcagtgct
    21421 gcagcaatga cagccgcaat atatccattc actttaaacg ttttggcagc agaaaaagca
    21481 agaaagattg gcagaaaata aaagacggca ttcgacgcag cagacaaaat atgataagtt
    21541 ccgcttgttt cactaagcca gccaatcgta gtcgctaaaa caaccagacc ttttaatata
    21601 ccagaaccgg caaacagcgg aatgatcggc gtaaagattt ccgatatcaa accaaatagt
    21661 ttttcgatcg gatttagctt ttctttcgga gctgaatgat cgatttgacg cgtcaattcc
    21721 ttgtaagcag gagccaatcc ctgatccatg attatttctg catatctatc gagtaaaatg
    21781 aaatacttaa tggaagggat ttgctgtttt aatttttctg cattggctac tggctgtttt
    21841 ttcaatttga tacgaagccg agtcgcacat ggaaggacag aatcaatatt gtcttttcct
    21901 cccaactgtt caatcagcat atgaatctgc tcggacagta ccatctgttc aggattcatt
    21961 gtcgtttcca ttagaagacc actccactca ctaatataat attggcgtaa ggtgtcattt
    22021 cgcctgtacg aataaatgca tgacagttat tcaactcttt tttcatttct tcatgtggga
    22081 taaagctaat cggtgtatct ggtaacaata atttgatttt ttccaatatt tccgggttca
    22141 ttgtctttat ttcttccgcc aattcaatcg attgtacctc aagttcttct aaaacgtttt
    22201 ctaaaacaga taaaaaactt ggaagaccgt ttgagacagc caaatctatt ttttcagtac
    22261 caaaggggac aggcatgcct gcatccccga tacttaattt atcaaagtgc cccatttgtg
    22321 cgattactcg tgaaatatct gaattgatta cttttccttt tttcatgttt gtttcttcct
    22381 ctcattgtta attttagact gctgattgtt ctaaagcttc gcggtaaggg atagaaggtt
    22441 gcgcaccata agactgcaca gcaacagaag aggctagatt tccataaagg atcgcttctt
    22501 tcaagttact aaggtctggc tgaagtgtgc tgcttaatgc gccaataaat gtatcacctg
    22561 cggctgttgt gtcaacggca tccactttaa aagcagggac gataccgtgt tctttttctg
    22621 tatgatagaa agcgccggca ctgcctagtg taatgattac tgttccaata cctaattcat
    22681 ggagtttttc ggctgctgcg accaaactgt tatgatctgt cactctgatt cctgtaatga
    22741 tttctgtttc tgtttcattt ggtataatga tatctgtctt ttcaagtagt tcagtaggaa
    22801 tcgtttcacg agctggtgca ggattcaaga tcgttgtttt tcctgcgtct tttgcaatag
    22861 aaaaagctag gatcgtcgca tctaagctac tttcaaactg agcaataata aagtcactgt
    22921 ttgtaatcag ttgcttgttt tttaggactt cttgttcatg gaaagcatta tttgcccctg
    22981 catggatcaa gatactattt tctcctgctg cgtctaccat aatcattgct tgtccggttg
    23041 tggcgccttg gatttcagct atcccagatg tatcgatgtt ttcttttgtc agtagatcta
    23101 ataactgttg tccttcgctg tcagccccaa cgcctccaat aaaagatgtc cgtgcgcctg
    23161 aacgtttggc tgccactgct tggttggcac ctttacctcc gccagcgtga aaaatctcat
    23221 gcgcatgcat cgtttcgcct ggcttgggca tgttggttaa acgaagggta gtgtccatat
    23281 taatacttcc aattactgta accgtgttca ttttacaaac ctccaactat ctcatttatt
    23341 ttgatgggaa aattattttt taaaacgttt tagtaaaacg ttttaatagt tcgataaaaa
    23401 tatagcacac cttgaaagct atttcaaggg tgcagttgaa aatctttttt ctaatcgtac
    23461 aggtaatctt ttttcttccc attctttttc tgggaattgg atccgatcta acaataactt
    23521 ggcagatgtt tgtcccaatt cgaagattgg ttgtgcgatc gttgtcagtt tcggcttgat
    23581 atactcgcac atatctatat tatcatagcc aataatgctg tagtcttcag ggatgctttt
    23641 tccagcttcc tctaatccgc gatataatcc aaaagctaat tcatcattta aagcaaacac
    23701 accagttgca gcagaatcaa gaagttctgc tgtgatctgg tagccacctt gttttgagaa
    23761 ctgtgtaggt aagaggatca gttggtcgtg agggatttgg taaacatcaa gagcagattt
    23821 aaacccctca atccgagcgt ggacattttc cggtggattt tcaggataga caagggcgat
    23881 cgtttgatgt cctagactaa gcaagtgcat gcccgctagg taaccaccgc gaaaatcgtc
    23941 tgttcgtacg gcatcactaa atccttcaga ttttttctga tccaaaacaa tgaagggccg
    24001 gccttgtttt ttcaaatttt cgttgattgc atctgtagag acagcagaag tcgcgataat
    24061 gaatccatcc acacctcgac gtgtgagctc ggctagatat tcgatttctt tttgatgatc
    24121 agaatcagca ttacataata tcgtgacgaa attctgctta tacaagatat cctcaattcc
    24181 tcgcatcagt gtactgaaga atggattcgt gatatcagga acgagtacac caattgtttt
    24241 cgtttccttg gtaatcattt gtctagcaaa atagtcaggc tgatagccta attcttcttt
    24301 ggcagctaaa actttctgaa cagttttcgg actgaatttc tccgtatttc catttaatat
    24361 caaggagaca gttgtgattg aaacgccgga aagtttagcg acttctttga ttgtgatctt
    24421 ttttttcacc atgttcatac tcctatctaa atcctgtggt tattttccca tgaaatcaaa
    24481 aagaagtaaa tgaaaatcac gtgtgccagt acttatttgc tttgttggat agcttcgatt
    24541 atttgtttcg ttgagcgtac tcgtgcagtt ttcggaaata tcttttttat gaacaattga
    24601 tgggattctt tgtcacgatc actgcaagca tcttcagcaa gcacgatatg gtacccgtgt
    24661 tgaaaggcat ctaatgcagt agcatataca ccgtttgttg tcgctacacc gcttaaaatg
    24721 atagtttcga ttccacgccg gcgaagttga agatcaagag aagtaccaaa aaaagcactt
    24781 ggattgtatt ttgtgatagt caacatatta tctgtatctt tcaaagaatc cttcaaaagt
    24841 aaatcagtga attctggagg tacagaagca gtcattccac ccatatcagt ttgttgactc
    24901 aaataaggga aagtggagat gtctacgttc actaaaactt tcaaggcagc ggtacgtttc
    24961 aatgttctta ctaacaaatc attgtttgtc agaatagatg ctgcagtgtg tggagagagt
    25021 gtaccgttat ttacgatctc tttttgcatg tcgatcgcga caaaagcagt ttttgataaa
    25081 tcaaaaatca tattttcctt taaatcattc atgatgttcc ttctttcaaa taacaatgtt
    25141 ttttatgtaa gtaaatcata aatcgtttat ttgtcaacta aattaaagga ttcctttctt
    25201 tagtctagag aaattcagtg gtaaaataaa taagtgtgag aaaacgcatt cgatgcatag
    25261 atatagcgag aaatatcgtg aaaatggagg aatcagagtg aagtatgtcg aagtaaaaaa
    25321 tgaatacaaa cgatacaaaa tgggagaaac gaccattaca gcaaatgacg atatttcttt
    25381 ttcaattgaa aaaggcgaat tagtgattat acttggtcca agtggagcag gaaagtcaac
    25441 gattcttaat attttaggtg ggatggatac acctgatgaa ggtcagatca ttattgatga
    25501 cacagatatt gcgcaatttt cagataaaca gcttactgct taccgacgaa cagatgtagg
    25561 gttcgttttc caattctaca atctcgttcc taatctaaca gcaaaagaaa atgtggaatt
    25621 ggcaacggaa gtttctcctg atgcgcttga tcctgtagag gtattgaggc aagtgggcct
    25681 agctcatcga ttgaataact ttccgtccca attatcagga ggcgaacagc agagggtttc
    25741 aattgctcgt gcactagcta aaaatcccaa acttctttta tgtgatgaac cgacaggcgc
    25801 gctggatttt gaaacgggca agcaagtatt gaaattattg caaaacgcta gcagacaaca
    25861 cggcaatacc gttttgatca ttacacataa ttctgcttta gcgcccatcg ctgatagagt
    25921 cattcacatt aatgatgcta aagtaaggtc tgttgaatta aatgatcatc cttcatccat
    25981 tgatgagatt gtatggtagg gggattttat gaaaacaaga acatatctta aagcaggttt
    26041 tcgcgaaatt cggcaatcta aaggacgatt cattgcaatc atccttattg tcatgctagg
    26101 aacactttta tttgtaggtg tcaaaactgc tggacctgtc atgcaaaaaa cgatggatca
    26161 ctatgttggt acagcaggac tttctgattt gcaaatcgtt tctactggtg gcttgacaca
    26221 aaaagatatt gctcaagcag aaaagatccc agatgcaaaa gtagaaacag gaaaacagat
    26281 ctattattcc aatactggca aaaacgaagt gattcaaata ttttcttata acaaaaataa
    26341 caagcagaac aagctacaat taactgatgg aaaactcccg gaaaaaccaa accagcttgt
    26401 attggatgaa aaagcggaag atgaagggta taaaatcggt gatatttatc agattgattc
    26461 agatgaattg aaagaaaaag agtataccat cacgggcttt gtccgttctc cattgttcat
    26521 caacaatttg gagagaggat acgcgaacgt cggaaatggt agcgtcgatt attttgtcta
    26581 tttacctgaa tccgttttta aaacagatat ttattctgtc ttatatcttg attttacaaa
    26641 cgtaaagaat ttgaatacgt acagcaaagc ttacaaagat aaaatggagc aaaatcaaga
    26701 aaaggcagaa aagtatttac aagatcgacc cgaagaacgc ttagaagagc tgaaaaaatc
    26761 ggccaacgaa gaattagaac cggcacagca aaaaatagcg gatggcaaag cacaaacaac
    26821 gcaagcaaga acacggttag aagatgctaa aaaacagtta gcgcaacagt cagcaactat
    26881 ccagcagctt cctgaagcgc aacgaacaat cgctgttcaa acattatctc aacaagaagc
    26941 tcaacttgct gaacaagaaa ggcaattagt tgagaaagaa cgtgaactgg cctctgcaga
    27001 ggaagaattg aatcgaaatg cagaaaaaat tgaaaattta actaaaccat catacctata
    27061 caatgaacgt tcagaaaatg tcggttttca agaatttggt gatctgtctg atcgtatcgc
    27121 tgcgattgcc aatgtttttc ctgttttctt tttctttatt gccgcattga tcacctttac
    27181 gacaatgaca cgtatggtag aagaaaaccg cagggaaatc gggacattaa aggcgttagg
    27241 ttatacgaaa gttgaaatcg caaaaaaata tgcaatctat gcaagtctag cctcagggat
    27301 aggtattgtt ttaggaacga tattaggaac gaatcttttg ccaaggatca tttttgagct
    27361 gtctaacgag cggtatgata ttgggagtgc gcttattttt tatgactggc cgccgatttt
    27421 acaagcagct gccgcattct tcattgccgc atttggcgct gcaatgatcg ttttattcaa
    27481 agatttaaga gaacggcctg ctgcattgct tcaaccgaaa gcaccgaagc cagggaaacg
    27541 catttttctt gaatacatca ctccgctgtg gtcacgcctt agctttaatc aaaaaatcag
    27601 ttaccgaaat ctcttccgat ataagtcaag aatgcttatg gcgattatcg gaattgctgg
    27661 atgtgccggg ctgatggttg caggcgtggg attgaaagat tctctaagtt ctgtttcagc
    27721 caaacaattt ggaacgatca ttgactatca agcaatcgtt acattggatt catccacgga
    27781 agatagtcgt caaaaagcag cggatatgct gcagaagcaa gaaaaagtaa atgagatcct
    27841 agctattcat actcaaaccg tagaattgag aaaaaaagga catgcttcgc aaagtctgac
    27901 agtgatcgta ccaagtaaaa cagctgattt ggatcgtttt atccatttaa ctgatcttga
    27961 agagaaccca cgctccttgc ctaaaacagg aatcgctatt acgcaaaaag tagcagaact
    28021 atttgactta tctacaggag atcatatttc gctttatgat gaagatcaaa aagaatggaa
    28081 agtcgaagta aaagaaatcg tgaaaaacta tctaggtagc tttatttata tgtcccctga
    28141 atattatgct tcagttactg atcaatcgat gatggataat gcatttttgc tgcgtacaga
    28201 taagatgaca acaaaagaag aagaaatgct ttccgagaaa ctgcttgcca cagacgctgt
    28261 gacaaatacg tctttcatgt ccaaacaaat cgaaacgcaa gaagaatcaa tggcgaactt
    28321 agactcggtt gtcttaatct ttgtcgtatt atcaggactg ctggctttta tcgtcttgta
    28381 taatctaact aatatcaata tttccgagcg tgtacgcgaa ttatctacaa taaaagtcct
    28441 tggatttttt gataaagaag tcacgatgta cattgttcga gaaaatatca tctttactct
    28501 tttaggaatc atcggaggat ttggtgtagg atatatattg actgacttca tcttgcaaca
    28561 agcgtctatg gagaatgtca tctttccatt agtgatcaca tggtgggctt atgtcttatc
    28621 agcaggatta actatcgtat ttacggttat tgtgatgatt gttacccatt tcaaattaaa
    28681 gcatatcgat atgattgatg cgttgaaatc gaatgaataa aaacagaggt tgtgaaatca
    28741 gcggtttgac ctgagtatta gagaaacaaa cggcaaaata gtttttcata ttttgccgtt
    28801 ttttgagcta ataccgaagg acagctgatt gaacaccatg aatcagaggt tgtaaaaaag
    28861 ccgtcctgca tcgagcaaaa agaatactca aataaaatta gctcctcatg ttttacattt
    28921 gagaagactg attgccgtag ataaaagctt ttgaacacca tttatagaag aaacaagaat
    28981 attttgctat tttaaacaag aggatagaca aatacatgtc tgatctcttg ttttttattg
    29041 ggcaacagac ctcttagaat gtataaagat aaattttaca aacgctttcg gttcagttat
    29101 aataataaat gataacttgt atatataagt tgtggtaggt aataaatcag ttgaatggag
    29161 gtaaatatcc atgacattta aagacaaagt catttaccaa atctatccca aatcttacaa
    29221 agacagtaat ggtgatggaa ttggagattt aaaaggaatc aaagaaaaac tcccgtatct
    29281 gaatgatcta ggcatcgaca tgatttggct aaaccctatt tatccaagcc ctcaaaaaga
    29341 caacggttat gatatctctg attacacagc catcgatcct atctttggta cgatggagga
    29401 attcgaacag ctaatagccg aagcaaagaa ataccggata gaaatcatgc tcgatatggt
    29461 gctgaatcac gtttcgacag aacatgaatg gttcaaaaaa gcactagcag gggaccagca
    29521 ttatcaggac ttcttcattt taagagaaga gccaacggat tgggtatcaa aatttggtgg
    29581 aagtgcttgg tcgccatttg gagatacggg gaaatattat ttgcacttat atgatcggac
    29641 acaagctgac ttgaactggc ggaaccaagc agtaagagag gaactattca aagttgttcg
    29701 tttttggatg gaaaaagggg taaaaggatt ccgtttcgat gttatcaatg ttatcggtaa
    29761 agacgaacaa ttgaaaaata ataaagaaaa tgatggcaaa cctgaatata cggacaaacc
    29821 aatcacccat acgtatctaa aagaattgaa ccaagcaacg tttgggacgg attccactat
    29881 catcacagtc ggtgaaatga gctctacgac gatcgaaaat tgtatcttgt atacagaacc
    29941 tagtagagaa gaattatcaa tggtctttaa tttccatcat ttgaaagtcg attatgaaaa
    30001 tgggaataaa tggtctactc ctccatttga ttttgcagaa ttaaagcgac tatttcatac
    30061 atggggagaa aagatgagtg agggtaacgg ctggaatgct ctatttttta ataatcatga
    30121 tcagccacgt gcgctcaatc gatttgtagc tatcgatcag tatcgaaaac aaggagcaga
    30181 gatgctagca accatgatcc atttgaaccg tgggacgccc tatatttata tgggagaaga
    30241 aatcggcatg atcgacccag actattcatc gatttccgac tatattgacg tagaaagttt
    30301 gaatgcttat aagttactcc ttgaacaagg attcacagaa aaagaagcgt ttcgtcggat
    30361 caaagcaaaa tctcgcgata actctcgaac acccatgcaa tggtcagaca cagaacaagc
    30421 aggatttact acagggaaac cttggttaaa agttggaggc aagctagaag agattaatgt
    30481 agaaaaagaa ctatcttctg aagattctat tttgtcctat tacaagaaat tgattcggct
    30541 gagaaaaaca tatccaatcg ttgcacaagg agattatcat gcttatgaag caaatcatcc
    30601 ggaagtttat ggctttcttc gccaatttga aggacagcag ctgcttgtat taacaaattt
    30661 ctatgataaa gatacaagga tcgccatccc agaaggattc acagaagcca atattcttat
    30721 cgataattat tccgatactg ttcttcatca aagtctggag ctaaaaccat accaggcaat
    30781 tgctttgctg aaaaattaaa acaaaaacac aaaacgtcgt tcctacgagc tattctcgta
    30841 agatcgacgt tttgtgtttt atttaaaata atcttcttca gaaaatgaag agttttcata
    30901 ctctccttta tcgccataaa atttattgta gcgttgtttg aatttacgga aaataaaagt
    30961 aacgagtaat ttgactaacg tatagatagg aataccaaaa ataacgccca caattcccat
    31021 tagatcaccc atgacaagta aaacgattaa gatcgtaatc ggatggatct gtaaacgatc
    31081 acccattact cgaggaatca ccaagtcacc gtggagaagt tgtacggcaa accagacaat
    31141 gacaaatttg acagccataa aagtagagtc ttggaatgca atgatcaaac ctggaaagaa
    31201 ggcgataaac ggcccaatat atggaatgat acataacaac ccagcagaaa tagctaaaac
    31261 actagcaaat tctaatccga tcagtaagaa acaaacccag tagactgctc caaggataaa
    31321 tgaagcaatg atttgcccct ttagaaatga acccaattgt tgattagcaa tctttgtcaa
    31381 atttttaaaa tctgtacgaa atgctggtgg gacaatcgct aaaactgact gataaaattt
    31441 ttgaggatct ctcaaaagga aaaaagcgac aattggacca gtaaataaag taataaaaat
    31501 agctgtaaca gttgaaaaaa tgttgctgat ccgttgtgcg ccactttccc aataatttcc
    31561 aataaatcca gctatatcag acgtcacgtc gtcaatagaa gcgaaaaatt gattaaatga
    31621 atcggcaaaa ggtgttttct ccaaaaattg tcggagactc tcttgaaaat cttgaaacaa
    31681 atctggcaaa ctactgatca atgtctttgc ctgttcttca agaaaaggaa taagccatat
    31741 gcttccaagc cccaataaaa ataaaataat cacataaacg ataatgactg acaagttcct
    31801 cgaaaggtgc ttgctcaaac gtttgatgac tggttccaaa agataataca agatcagtgc
    31861 aaacaataat gggggcaaga tagtagcgat aatgattccg acaggcttga atataaacga
    31921 gatctgatgg aaaaaataaa tgatcagcgc aagtaagata actattccta aaacatagta
    31981 agaggttttt cctcctaaaa agcggatcaa acgattcgtt gtatctgttt cttttttcac
    32041 tctttttcct cctttatcta taaattcatt atagacaaaa gcgggtataa aaggaaaatg
    32101 atagaggttg attgcaaaaa tatcgtcaat cattcatata aactttctca tcaattaaaa
    32161 taagaaaacg cttgacagtt cttttaacca tatgataaat taaatcctgt tgctaaaacg
    32221 taactattac ggtttgcgaa aagcgaatcg gaacattcaa atgaaagaag atgagaagag
    32281 gagtgtcata aatgaaacga ttgataggta tattggccat gctggtttta gcaggtatgt
    32341 taactgcttg cggggcaagt gggaaagccg aggacagtaa agaaaaattg agcgtgatga
    32401 cgacctttta tcccatgtat gattttacaa aagcaatcgt cggggatgaa ggagaagtag
    32461 agttattgat tccagcaggg actgattccc acgattatga accttctgct aaagacatgg
    32521 caaagatcca agatacagat atctttgttt ataatgatga aaacatggaa acatgggtcc
    32581 ccgcaatcca aaaaacatta caagaaggca atgtccatac gataaaagcg acagaaggta
    32641 tgcttttact tccgggaagt gaagaaggac atgatcatga ccatgaacac ggggaagaag
    32701 gccatacaca tgagctagac ccacatgtat ggctagctcc aagtttagca atcaagcagg
    32761 tagcaaacat acgtgatcag ctgattgaag cttacccaga aaaacaggaa gtatggacaa
    32821 aaaatgcagc agcctatacc gagaaacttc aagcactcca tcagctttat caagaaacct
    32881 ttaagcaagc aaaacaaaga tcttttgtca cgcaacatgc tgcattcaac tatctagcgt
    32941 tagaatatgg attgaatcaa gtatcgattg ctggtctttc ttctagtgaa gaaccgtctg
    33001 cggcgagaat tgctgaactg aaacactttg ttaaagaaca tggaatcaac tatatttatt
    33061 ttgaagaaaa cgccaaagat agtattgcca gaacgttagc aaacgaagca ggtgtatctt
    33121 tagaagtatt gaatcctttg gaaggattga cgaatgaaca aatagaaaat ggagaaaact
    33181 atctttctat catggaagca aacctcgaag cgttgaaaaa aacgacagag acagaaaacc
    33241 cgctagaaga tactttgacc cccaaaaaag aaaagagcgt ctacaacggt tattttgaag
    33301 acgaggatgt acaagatcgc tctctttcag attgggcagg aacttggcaa acagtagata
    33361 cgtatgtaga agatgggact tttgatcctg tttttgaata taaagaaaaa ttgaatcagg
    33421 ataagacagc acaagaatat aaagaatatt atacgaaggg ctatcaaaca gatattgctt
    33481 caatcaagat cagtgagaaa gagatggcat ttacgtttaa gaatggaaca acgaaacgtt
    33541 caaattatcg ttatgtaggg aaagaaatct tgacctacga ggcaggaaac cgtggcgtaa
    33601 ggtatctatt cgaagcagaa gataaggaaa gtggcgcttt tagatatatt caattcagtg
    33661 atcacacgat tgcaccggct gactccgaac attttcattt atatgcagga aatgaaagtc
    33721 aagaagtatt gctgaaagaa atggagaatt ggccaacatt ttatccagat acaatggatg
    33781 gacgtgaaat tgcacaggaa atgcttttgc actgataggc aaaaaagaac cgaagggttt
    33841 ggatcggctg gtcatttagt cggtccaaac tattcggttc tttttatgaa atcaatttct
    33901 ttaatccacg gatagaggct gggatggtct cgccgtttgc caacgtgact tctctggttt
    33961 ttttattaat cgaccgcaca ttttgtgtat tgataacata ggatttatga caacggataa
    34021 aattccgatg cattttctcg atttctttga tcttctcata gaattgtaat tgggcatgag
    34081 tcaaatggac ttccagttta tgtggcgctc ctagcgaagt agaaaaatat tggatgtctt
    34141 tgatcggtaa aaatttgatc tcatcattaa actctatttt gaagatatct ttttggattt
    34201 gggacatttg aaaatcttcg aaaattttgc tcaggatatg ctcgacattt tcttttaaat
    34261 cataaagatc ttctttgacg atataatcag tcggttcgat gttgcttttt aagattttca
    34321 tcgacatttc cgtatgggaa gtcacataga ttatttttcc taaaggatct aattcacgga
    34381 tcaatttacc gatcgctaca ccatccattt cactgtcttc tagttcgata tctaaaaagt
    34441 aaataccaaa agaagaagtt ctgatcttgg ctgcattgag aagttcttcc ggatcagagg
    34501 tagaaatatg gacataactg tctaagtttt caatcatgat tctatttttg atcaccgttg
    34561 ataaaacttc taattgtttt ggatcgtctt cacaaatata aatgggaatc atatcattac
    34621 tcctttattt tatttgtata taaatcgttt gagaaaaacg ctggttttca attgatgttt
    34681 caaggaaaag ataaggattg gtctttgtca attcatccaa gataaacaag ccaaggcctt
    34741 cgttttttgg atttgttttt gttgagaacc ctttttgttt caactggttt aacggtaatg
    34801 tatttgttgt tgtattgatc acacgaatca tcacggaatg attcttcttg agtatggtca
    34861 attcgatttt tttcttcttt gcagcactag ctccttcaat cgcattgtcc aagataatac
    34921 caagcatacg tacaaggttt accgtatgtt tcgtatccag ttcgattggc tcaggaaaat
    34981 gaagctggac gatcaaccct ttttcctgtg ccatcatgag ttttaatgac agtaaactac
    35041 ggatctcagg aactttcata ttatccaaca tacttagatt ggccggaaaa aggttcatca
    35101 tctggcgtgt aggtaatatc gtttgatcat aataagaccg taatccttct aagtcgcctt
    35161 ctgaaatata gctgtgaaga gaaaaaagca gattttggta atcatgtttg aattctctca
    35221 agtcttgata gtttttttct agatctttga tataatcctg ttgcatcctg acttgttgct
    35281 ctaataactg atcagctaat ttcttattag agattttcca ataataaacc aagatgatca
    35341 ggccaacaca cagatataga atgaaaaaac tcgtattcaa agctaagaca tcaggtgttt
    35401 caccagaaaa acgggtataa aaaataatga tatagtatgt aaataatatc aatgtcccga
    35461 tcgtaccgat catacggcgg tccacagtgt gaaaccaagt ctctaacgat ttttttaaac
    35521 aaaaagctaa taaagcagat aaaaaagcgg ataagacaat gtgaaaatac tgcaatgaat
    35581 ctcctggttc aataaccgaa gatttcaaaa caaaaaaatc gccgatgctt gttaagtgat
    35641 ctcctaaaag cgacgtcaat aacccaaaag gaagcgcgta aaccaattgt tgacgcgaag
    35701 tcgaaaagaa gaaaaaagta agcatcagca ttcctatagc aattagagca gtaaaaatcc
    35761 ctaaaatcga atagacccat ccaatcaaaa tacaagaaat cagaatgact cccgcaaaca
    35821 gatatttgta tcggatgtga tggtcaatca ataagataca aattgtgtaa atcaaatatt
    35881 gcaacgctat ggaccataaa aacataaagt acagctccca ttgattttta ccaaattata
    35941 gcacaattgg cgtattattt ttttaatttt tcagggattt tgaattcaga actcatacca
    36001 aagcagcagc cagatacaaa aaagtcagat aagttagtta attgtttaat gattgttttt
    36061 ttcatgaaag attcctccaa aattcatcaa gtagtttgta ataggaagca acgtagctat
    36121 ttctaaaaca atcccaagta gcaaataggt agaaaagtaa gaagctgaga aaaataacgt
    36181 aatgagagaa aaaattagaa acatgctgat cgccttcttt aataatgggg gacgtttctc
    36241 gggttcaatg ggattgattg ctgtacctct tgatccatag cgaagcagta acaaaaagca
    36301 gctgattaac aaaaagacca tcattgggaa ggagatagtc aattgcagat gtttcatcac
    36361 caagggaacg ccccataaat aaagtagtgt ccacagcaga caaacgaatt cagagtcgga
    36421 atgcaatcca taagcatatc tgcggatgca gatataagaa agaaaggtga ttcctgtttc
    36481 cgcccatgag cctgtcacca gagaaaagac tagaacgacc gctagttttc caagattatt
    36541 caataaaact tctaagacaa attttacttg gacatacacc atatcttctt ccgatttgtc
    36601 tccgctgaat aaggcttcta ataatttgtt cacaacttta tctttcatct catacgtacc
    36661 tccaccacaa attttatgct aaaaaaaata aaaaacgtct tttgtttcat aaaaagtggt
    36721 tttgacatca aaaccgtcat gatctcctaa aaaaatcttc gttttccaag aaccttacca
    36781 aaaaaagtga cagttttgat acgaaaagta ctttttatga caagtttgtg ctttttcgag
    36841 tgatttatgt taaaaaatta ctgaccttat aaaaggtgat ggagggattc ttatggaaaa
    36901 tgaacaattg tttgaagtcg gcgaaacata tgcatttaaa tctgatttat ttgaatcacc
    36961 aataaaagga aaaatagaaa aaatctatga aaatagtgtg atgatacgtg tcatgaaatg
    37021 cgcagtagaa gacaatgata ccgctcataa tctgcactgg cagttagttg tacgcaaatc
    37081 aagtacaata gaagaataaa aacagataaa atgttgagag cataaagagg ttgtgagtct
    37141 cccgtttagc tctgagttac aaggaataat gttaaaaaac agcttttcat gttttatgaa
    37201 tattgccttg tgaccgaggt gttggcagga gaatactgtt tatttgaaaa aagggctgcg
    37261 acaagacttt tgtcacagcc tttattttca tgaataggag ttacttttgg ctacttttag
    37321 agaagtagct tttaaggagc agataaagtc aaggctttta tttctataaa tgaagtagta
    37381 aaatgaagaa cagattttat tacaggaagg atgccgataa ttgtgatcat tgttgcagga
    37441 acgatcggtg cagggaaaag cacactaact gaaatgcttg cccaagattt agaaacaaaa
    37501 cctttttatg agaacgtgga agataatgaa gtattgccgc tattttattc taatccagaa
    37561 aagtatactt ttttattaca gattttcttt ttgaacaaac gctttttggc aatcaaagat
    37621 gctttcagcc atgatgacaa cgtactagac cgctccatct acgaagatag tatgctgttt
    37681 catttgaatg cagatttagg gcgtgtttct gaagtagaag taaagcagta tgaaggtctt
    37741 ttagaaacga tgctgaagga gttagaagaa atttcgccac aaaagaaacc agatctactg
    37801 gtttatattc gtgtatcctt tgaaactatg ttagcgagaa tcaaaaaaag aggccgggaa
    37861 tatgaacagc tagaacaaga tcctgaactc tattcctact ataaagaact caatcgacga
    37921 tacgaagagt ggtatgaaca gtttgatata tgtcccaaaa tagtgatcga tggggacaaa
    37981 tatgatttcg tagccgatcc ggcatgtgga gaacaaatcg tacaagaaat caagatgcgg
    38041 gcaaagaaaa tgacagaaga ggcagatgcc cagtcgatcc gctacactac aaataacccg
    38101 aaagcaaagg gaatctttac aactaaataa tataaaaact aagcaaattg ctcactcgca
    38161 gatatttctc tgtgagcagc atcttgctta gtttttttga ttcattgaga tcaaatagtg
    38221 aaccgattcg tatgataaaa ttgccataaa tgaaatgtct gagtgagggc catgatcaaa
    38281 aataaaatgg aaacgatcaa aatcgaatgg gcgccaaaca gatgatgaca gaaaacaagg
    38341 acaatagctc ctgcgataaa ggcaagaacg acacttagat agtggaaaaa tcgatcaaaa
    38401 tctgcttttt ctttagaaat acaataatca tataagttcg ttgctacccc ttttaaattt
    38461 ccagttgtaa ataaattagt gtaggcacgc ccgttgattt tatcaaaagc tacccactgg
    38521 atcgatgcag taaaagcaat caaaatcgtt actaatgcgg gatgctcatg gacgggtgtc
    38581 aaactaacaa agaaaaaaat cactgcttca taatatagaa caaacaagcg ccagaaatgt
    38641 ttttcttttt ttctaaaata atcaattaga tactttgtta gaagaatccc taagaaaaag
    38701 aaaagggtag acaatacttt tttaccaaca cttagccatt ctccgtcaaa tgcttggatg
    38761 atcgatagaa tgatattgcc agtttgagca gaggcaaaag caccatattg gatataagta
    38821 taactgtcca tggctcctcc gataaatgtc agcaaaagtc ctattgtacg cccctcgtga
    38881 aaggaagtct gcattttttt catcaccttt tatgaatatt tttgtcttcc ttatcttagc
    38941 ataaacgatt gaaaaaaagg aaaaattttc agaaaactaa aatcatatcg cttcccattt
    39001 tcatttttag aaaggtctgt tagtataaag aaaaaataga caacgaaact ttaaataacc
    39061 aacttgaaat aaacaaattc aaagaagaaa gatgggaagg aaatcaatca gttgaatgaa
    39121 aaagaaattc tccgattaca aaaagaattg aaacgctggg agaacaagta taaaaagctt
    39181 caacaagtct ttcggctaca agaagaaatc attgctacct atgaagcggc ttatggcgaa
    39241 gcacccggac ttattgagga agaagtacaa gtaaaagaaa gtgaaggaaa cagctctttc
    39301 tttaaaatga tggagaaacg ttcctataat cgcttcaatc aagaacaaaa agcagctatc
    39361 acatatgata tgaagaaaca tcttcgaatc atagcaggag ctgggagcgg caaaacgcaa
    39421 acaatctgtg caaaagctgt ttatctgatg acgcaaaaac aagtagacga agaacgcata
    39481 ctaatgatca cttttacaag gaacgctgca aatgaactaa aaaaacgagt agataatttc
    39541 agccagagaa aaaccgctgt ccatatcgga acattccata gtatcttttt taggatatac
    39601 aatgaaattt gtcgaaaatt tcctgaagta gcaatgcaag gaatacaagg agacttttca
    39661 aaggatactg cacaaaaagt gaatgctgtt ttgcaacagc tgatacgtaa atataatctt
    39721 tatatatttg atcaatatgg agaaaaaacg attgcttctc ggttagacta ttggcagaat
    39781 atgaaccttt cagtagaaga aatggttcag ttagttaaag aaaaatatga ttccattgat
    39841 aagaatgcac gacagccgat atcagaacga ctgtatggat tgctcacgga actccaagaa
    39901 caaaaaagaa gccagcaatt gctagagttc aatgatgtac tccaaaatct gaaaagcgct
    39961 ttggagaatg aggagatcca gcattatatc ggccaaaaat atgattatct attcattgat
    40021 gaatttcaag atactaatcc acttcaatgg gaaatcgtga aacggatgac aaaggaaaac
    40081 aggatcaagt tgattgttgt tggagatgat gatcagagta tctatggatt cagaggatca
    40141 gagccagcat acatcaagaa ctttgaaaaa gaatatccta ccaaaactct tttcttatta
    40201 actaattatc gttctcgggc agctattgtc caaggagcaa atagattgat cagctataat
    40261 aaaaatgatc ggataccaaa ggcgatgatt ccagcacaaa aagaagcagg tgttatggaa
    40321 gcctatttgt tttctgatac taaagcagaa agtcagtggc taatcaaaca aattcagaca
    40381 tttattcaaa aaaatgggaa atataaggaa tcaatcatat tataccgatc agcttcacaa
    40441 acgcagcagc ttgtccaatc tctgctgaaa acgaatattc cttttgtact ggaagcagat
    40501 agtccatatg aaggtatctt tggtatcaag tctttccaac atttctatca aaaaattgtt
    40561 cattggcaaa cagcactgaa tctgcaaaag aaaaatcagg cctaccagca attgctccgg
    40621 cagctgatgg cagactgcta cctgaaaaaa agtgaatgcg atcaatactt ttctcctaaa
    40681 tatcaaaata tagccgttgc agattatatt ttgtcgataa gccccaattt aaaatcgaaa
    40741 tctgcagaaa tccgagagtt tgaggaagcg ttgcgtaaaa cagaccagca tcaagaaatc
    40801 tattcgcttg ttcaaagcta tcttcatctg ccacgtatag ctaaggaaat cgaccagagt
    40861 gatcaagaat ggatacgaga agaagtaaag cagcatgcta ctttcagaag actccaagcc
    40921 ttatcgcaag aaacccaagc gacagctaaa gaattaaaaa cccgcttgat tgcttacaga
    40981 caaggaaaac ttgatgcact atgtatccag agcattcaca aaagtaaagg tcttagttac
    41041 cgaaatgtct ttttgattgg ttgtaacgaa ggaagtttgc cttataacgg agcgacagaa
    41101 aagaaagtaa tcgactttaa tgaaataaaa gcagaaccag taacgacgat agaagaagaa
    41161 cgaagactat tttatgttgc gatgactaga gcaagaaatc gtctgtatct atgtgtacct
    41221 caaatgaaaa attcaagaaa attgaaagta tcgagattta tcaaagaaac cgggagtcgt
    41281 gtgaaaaaag gaaagtgata gaagagaggg tgtgacagaa gtggtcagtt tcaagaaaga
    41341 agaaggaatg tacgaaaatt gcttctcaaa tttttgtgaa atttcagctt attttcgaag
    41401 aagctatttc tggaacacag tttattcgga aaagggaggt gtgacaagaa ttttgtcaca
    41461 cctcccttat ttattttgaa aatgagaata ctattatcaa aatagagaat atatagaaaa
    41521 attctcatta ttcaaacgta aataatcagt tattttgatt ttttttatat tttattagct
    41581 aataattggt attttaaaag aaattactgt agaattggat tataattaaa aaggagtgag
    41641 aaaatgataa aagtatatac ttcgcctagc tgtacttcct gtagaaaagc aagagcatgg
    41701 ttgcaagcga atcaacttga atttgaagag aagaatattt ttgccgaacc gttaacagaa
    41761 agtgagatca aaaagatctt ggcagcgact gaaggaggag ttcaagatat tatttccact
    41821 cggtcgaaaa tttacgaaaa gctgggcata gattttaacg aattaacttt aaagcaaatg
    41881 atcgtcttat tcaaagaata tccttcatta ttgagacgac ctattttgat tgatgaaaaa
    41941 agaatactaa tcggatttaa tgaagatgag atccggtcat ttgtccctcg tgaaattcga
    42001 tgtgttgcca gaaatctgat ctatttacaa aacaataaca tcttaaattc atagggaggg
    42061 agccttcgtg gaagtgaaag tctatggatc agcgcactgt gtaacaagtc tgaaggcgaa
    42121 acaatggtta gaacaccatc aattgcctta tcaatttgtt gacttagaag aacgcgatct
    42181 aagtacagaa gaagcagaag aattatgcag ttttgaagaa gtgcaagcag aacagctatt
    42241 tgcgacttgg tctgaggctt ttcacaacat tgcgattgac ctttcctgtg taggaaaaaa
    42301 acaactgatt tttttatgca cacaacatac caatctcctc agacgtcctt tgattattat
    42361 cgatgaagtt ctatttattg gatacaatga gcagttgatg gaacagctta tcttaaataa
    42421 atagacttta actgttttaa tcaagtcact gagcatttca tacagttgta acagtcgaac
    42481 acttagcatc aagcgtgtta tgtgttcttt ttttgacttt atttttctac tatagagaca
    42541 tgctcaattt atgtggtcaa aattcaaatc taggtgggga tatagagtta aaaatatatt
    42601 gacatgtgta aatttacatg atatattcat ttctgttgtt ttaagtagca aagcaataaa
    42661 atagtatttt aaaaaacata taaaatagtt gttgacatgt tagaaatgac atgataagat
    42721 aactgagttg ttaaaaaaga accacttgtg aagcttgatt ctatcgaaaa aaacttcaaa
    42781 aaagttcttg acaaataaca aatgataagt tatgataaat gagttgctgt aagatttcga
    42841 aaaacgaaat caaaaaaact tttaaaaaag agttgacaat tacttgtctc tctgatatga
    42901 tataaaagtt gctacggcag ccaaaaacga atcgg
//
LOCUS       DACEDO010000016        42403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEDO010000016 DACEDO010000000
VERSION     DACEDO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.016"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(148..2301)
                     /locus_tag="IWO52_000954"
     CDS             complement(148..2301)
                     /locus_tag="IWO52_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0355236.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="IWO52_000955"
     CDS             complement(2595..3443)
                     /locus_tag="IWO52_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0355237.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IWO52_000956"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IWO52_000956"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0355238.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="IWO52_000957"
     CDS             complement(5944..6123)
                     /locus_tag="IWO52_000957"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0355239.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IWO52_000958"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IWO52_000958"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0355240.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="IWO52_000959"
     CDS             8271..10550
                     /locus_tag="IWO52_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0355241.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="IWO52_000960"
     CDS             complement(10643..11290)
                     /locus_tag="IWO52_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355242.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="IWO52_000961"
     CDS             complement(11469..12188)
                     /locus_tag="IWO52_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0355243.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="IWO52_000962"
     CDS             complement(12319..13470)
                     /locus_tag="IWO52_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0355244.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="IWO52_000963"
     CDS             complement(13687..14427)
                     /locus_tag="IWO52_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0355245.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IWO52_000964"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IWO52_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0355246.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /locus_tag="IWO52_000965"
     CDS             complement(14811..15404)
                     /locus_tag="IWO52_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0355247.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16035)
                     /locus_tag="IWO52_000966"
     CDS             complement(15388..16035)
                     /locus_tag="IWO52_000966"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0355248.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IWO52_000967"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IWO52_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0355249.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IWO52_000968"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IWO52_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0355250.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="IWO52_000969"
     CDS             complement(17481..18008)
                     /locus_tag="IWO52_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0355251.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="IWO52_000970"
     CDS             complement(18188..18973)
                     /locus_tag="IWO52_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0355252.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /locus_tag="IWO52_000971"
     CDS             complement(18966..19835)
                     /locus_tag="IWO52_000971"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0355253.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="IWO52_000972"
     CDS             complement(19836..21215)
                     /locus_tag="IWO52_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0355254.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IWO52_000973"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IWO52_000973"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0355255.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IWO52_000974"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IWO52_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0355256.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IWO52_000975"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IWO52_000975"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0355257.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IWO52_000976"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IWO52_000976"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0355258.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IWO52_000977"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IWO52_000977"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0355259.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="IWO52_000978"
     CDS             complement(25173..25397)
                     /locus_tag="IWO52_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0355260.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="IWO52_000979"
     CDS             complement(25450..26412)
                     /locus_tag="IWO52_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0355261.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="IWO52_000980"
     CDS             complement(26422..26862)
                     /locus_tag="IWO52_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0355262.1"
                     /translation="MESDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="IWO52_000981"
     CDS             27168..28133
                     /locus_tag="IWO52_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0355263.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="IWO52_000982"
     CDS             complement(28190..28420)
                     /locus_tag="IWO52_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0355264.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="IWO52_000983"
     CDS             complement(28506..29393)
                     /locus_tag="IWO52_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0355265.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="IWO52_000984"
     CDS             complement(29482..30705)
                     /locus_tag="IWO52_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0355266.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31801)
                     /locus_tag="IWO52_000985"
     CDS             complement(30722..31801)
                     /locus_tag="IWO52_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0355267.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IWO52_000986"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IWO52_000986"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0355268.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="IWO52_000987"
     CDS             complement(33659..33925)
                     /locus_tag="IWO52_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0355269.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="IWO52_000988"
     CDS             34404..36638
                     /locus_tag="IWO52_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0355270.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="IWO52_000989"
     CDS             36786..37106
                     /locus_tag="IWO52_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0355271.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37311..37535)
                     /locus_tag="IWO52_000990"
     CDS             complement(37311..37535)
                     /locus_tag="IWO52_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ0355272.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37733..39313)
                     /locus_tag="IWO52_000991"
     CDS             complement(37733..39313)
                     /locus_tag="IWO52_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0355273.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39714..41090)
                     /locus_tag="IWO52_000992"
     CDS             complement(39714..41090)
                     /locus_tag="IWO52_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0355274.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41218..42336)
                     /locus_tag="IWO52_000993"
     CDS             complement(41218..42336)
                     /locus_tag="IWO52_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0355275.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT        11990 a         9126 c         7323 g        13964 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttggatct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcagatatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggtagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctgattcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcatatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc gaaagcagaa
    37201 gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    37261 ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt ctagcttttt
    37321 gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg cgtgtcttta
    37381 tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat ttgctcgttt
    37441 ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc ttttttctgt
    37501 ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct ttctttaatc
    37561 atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga ttttaatata
    37621 gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa caaaatatga gaggctgttt
    37681 ttgtttggct attcttatta cttgatgcag ggcagcttgt gtcaagactt ctttacatca
    37741 aactttttaa ttttacattg ggatatttat ccgcaaacca gcgttctgca aattggttct
    37801 cgaataagaa caacggttgg tcgaaacggt cacgagccaa gatattgcga cttgaactca
    37861 tcttctcatc caaatcttct ggatcgatcc agcgagcgat tttgtgtccc attggagaca
    37921 tgattacttc ggcgttatac tcatttaaca tacgatattg gaatacttcg aattgtagct
    37981 gaccaacagc tccaatgata tattcttccg ttagatatgt tttataaagc tgaatcgctc
    38041 cttcttgaac taattgatag attcctttgt ggaaggattt ttgtttcatg acattttttg
    38101 cagtcacttt cataaacagc tctggagtaa atgaagggag ttcttcataa gcaactttta
    38161 gttttccttc gaacaatgta tccccaatct gataattgcc tgtatcatat acaccgatga
    38221 tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc tgccataaat tgtgtgacat
    38281 tgcttaattt cagtttttta tttgtacgtt ccaaccagac atccattcct cgttcgaatg
    38341 ttccagaaca gattcggaca aaagcgattc ggtcacgatg ggctggattc atatttgctt
    38401 ggatcttgaa tacgaaacct gaaaattctt cctcataagg acttaccatc tgatcatctt
    38461 gtgttttgtg tccataagga gctggtgcca attcaacgaa agtctctaaa aaagtctgta
    38521 caccgaagtt agtcaatgca gaaccaaaaa atactggtgt ctgatcgcca cgagcaattt
    38581 tttcttgatt aaatgcatct ccagcctctt tgattagttc tacttcttct aagacttgtt
    38641 catataagga attttcagtt agaggatgat tttcgggaag cagtccatct tcatccaatt
    38701 ctactaatcg ttcatcctga taattttccg gacgataaaa ctctacacga ttatggtaga
    38761 tatcataaag accttctagc cctttaccca taccgattgg ccaattcatc ggataagatt
    38821 cgatatctaa tagctcttct aattcttcta acaattccaa tggttcacga ccatcacgat
    38881 ctagtttgtt gataaaagta aatatcggga tgccacgttt ttttacaact tggaataatt
    38941 tctttgtctg tgcttcgatc ccttttgcac tatcgattac catgaccgca ctatccacgg
    39001 ccatcaaagt acgataagta tcttcagaaa aatcttcatg tcctggtgta tccaagatat
    39061 ttactcgttt cccttgataa tcaaactgca taacagaact agtaacagaa atcccacgtt
    39121 gtttctcgat ttccatccag tctgatttgg caaaattacc tgtttttttg ccttttacgg
    39181 tcccggcttg acggatagcc ccaccaaaca atagtaattg ttctgtaatc gttgttttcc
    39241 cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt gtccacttgt tgctttaatt
    39301 ttggattatt cataacctaa ctccatttct tccattcaat tatttatatt ttctctaggc
    39361 acagattgtc cgatctctta aagaaagaca atttcatata gggctgtgac aaaagccgtg
    39421 tagcagtctc tttttccgag taaacggcgt tctagagaca gcctatcagc aataagccca
    39481 cccaagccaa aaatacgaaa agctgttttt gcttggctgc tcttattact taatgcagga
    39541 tagctttttc acaacctttg tcaactacta caactgaatc agtatagcat ggattcagcg
    39601 aatgaaaaag ccaccaaacc ataagagtca aatatttttt tgtcagctga ctaaaagaaa
    39661 agacgtgttg cttgtttatt caagcaacac gtctttcata accaagtttc caattatctt
    39721 cttggctcgt catcttcgaa ttcttttctg aagtatcttc gttcttcttc aggttccgct
    39781 tctgtttctt ccggatcatg aaaaatgaca cggattttaa ggattcgaga accttccatt
    39841 tcttcagaaa ccagcgtaat attatcgaca tcaaacgata acttttcgcc ttcatccgga
    39901 atcatgccta atgctgtaat caaataacca gccatcgtat ctacatcact catatgcaga
    39961 tcactgtcaa aagcttcatt gaactcatca atcagcatac gtccttggat gatatattcg
    40021 tgttcatcga ttttttcata aagattctca acttcatctg attcatcatc gatttctccg
    40081 acgatttctt ctaaaaggtc ttctagtgtt gctaacccaa cgacgccgcc atattcatct
    40141 aataaaatag ccatctgatt ctgcgtcttt ttcatttcat ataacagatc atcaataaag
    40201 accgtttcag gaacgaataa aggctcctgc atgattttct tgatatctaa attatcaaag
    40261 ccgaatttat gggcagcttt tagtaaattc tttgtatgaa ggattccgac tactttatct
    40321 ttgtcttcgt tataaacagg gattcttgaa tagttttcgg ataaaacttc attcacattt
    40381 tcttccacag tgtcgttgat atcaatcata aatgcatccg ttcgtgggac catgacttct
    40441 cgggcaactt tcgtatcaag agaaaaaacc ccttggagca tttctaattc ttcattattc
    40501 aatacgcctt cgttttcaag catataacgc atttcatctc tggtcatttt tgaatcttca
    40561 tcatcaaaag tcatcggcgt gatacgcgcc aataaacttg tggaagcaga aagcagccag
    40621 acgaagggct tagctactac accaatcaag cgtatcagcc ctgaagtgaa ctttgcgact
    40681 tcttctgatt tatttaaagc aatacgttta gggtacaatt ctccaaaaac aatcgataca
    40741 taggtcaaaa tagccagcac aatgatattt gcaatatttt ttgccgctgt gccgccacct
    40801 aataccggag ctaaccgttc cgataaagta ttagctagtg aagcaccaga caaaatgttg
    40861 accaaagtga ttcctacttg gattgtcgat aggaaattat ttggattctg caatattttt
    40921 aaaagctttt gagatttcac atctccttca tcagcttttt gctcgattcg attcttattg
    40981 atcgaaacaa cagcaatttc ggaagctgct aaaaaagcat tgatcaatgt caaaacgatc
    41041 aataataaaa tctgcgctaa cagcgactga ctctcagggt cagcattcat aatcgcattc
    41101 tctccttata aaattaattt ttagtttacg atagaaaaaa acgtggcact atagccacgt
    41161 taaaatggta tcacaaagca attttctttg caataaaaac aagtgtacta aaactaccta
    41221 cgaggtcgta gcaattttct tcttgttttc attttgcttg tcttctttga cgattttgat
    41281 tatataggaa atgatcgtct tgcagattgc gtaaaaagga acacctaaga agataccaag
    41341 caatccggca atatttccag ctactagaag aacgataatg atcgttaatg gatggatttt
    41401 cagtgatttc ccaattacat taggataaat gatattccca tccaactgtt ggacgattag
    41461 cacaaccaca caacaaagta atgcttttac cggatcattg aagactgtta ctagaaatgc
    41521 aggtgctagt cctagataag gtcctaaata tggaattagg tttgtcaatc ccgcaatgac
    41581 cccaaataag aaagcataat ccacaccaat taccatatat cctaaaaatg tgaaggtacc
    41641 aacgaataaa cattcgattg cttgtccgct aatgtaatta gccaatgttt tattcagttg
    41701 ccccaataac tccacaattt ggtttcttct tttttctggg aaaaactgtt ggatgtttgg
    41761 cactagtttt tctccgtctt tcaacatata gaacaagatg aatggtgctg taatgattac
    41821 tacggttgcc gaagcgatcg ttccaacgat cgaacctaaa ctattggata ctccactaag
    41881 gaattgctga atgattgtcc cgtaagaaat gtctaattgc tcaaaatatt tattgatatc
    41941 tacttccttg aacaaaggca attcggctac ctcttttaac cagctttcta cctgtttgat
    42001 gaaatcaggc atattagatg ccaatgagga aatttgctgt actagacttg ggatgacact
    42061 aagtaaaatc caaataattg ctgcaactaa aaggagcagc acaattgcga cagcccatat
    42121 tcttttgatt tttgctttca tcaacagctt gacaatagga ttcaataaat agtaaagaaa
    42181 gcctgcaatc agtacaggtg caaataacgt ggaaaaaaat gtcccgatcg gttcgaaaat
    42241 aaagtttatt tctgatgaaa tcatgatcaa tgcagctatg atcaataatt caagcgacca
    42301 aaacattagt ttagactgtc taagtttttc aaacaataga tttcctcctt tctttcttac
    42361 tagtatatat tatattcgtt aaaaattctt ttattctttt ttt
//
LOCUS       DACEDO010000017        40644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEDO010000017 DACEDO010000000
VERSION     DACEDO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.017"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..1936)
                     /locus_tag="IWO52_000994"
     CDS             complement(53..1936)
                     /locus_tag="IWO52_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0355276.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2202..3077)
                     /locus_tag="IWO52_000995"
     CDS             complement(2202..3077)
                     /locus_tag="IWO52_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0355277.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(3186..4955)
                     /gene="aspS"
                     /locus_tag="IWO52_000996"
     CDS             complement(3186..4955)
                     /gene="aspS"
                     /locus_tag="IWO52_000996"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0355278.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(4970..6262)
                     /locus_tag="IWO52_000997"
     CDS             complement(4970..6262)
                     /locus_tag="IWO52_000997"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0355279.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            6656..7603
                     /locus_tag="IWO52_000998"
     CDS             6656..7603
                     /locus_tag="IWO52_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0355280.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(7692..8951)
                     /locus_tag="IWO52_000999"
     CDS             complement(7692..8951)
                     /locus_tag="IWO52_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0355281.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9122..9568)
                     /locus_tag="IWO52_001000"
     CDS             complement(9122..9568)
                     /locus_tag="IWO52_001000"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0355282.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(9579..11792)
                     /locus_tag="IWO52_001001"
     CDS             complement(9579..11792)
                     /locus_tag="IWO52_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0355283.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(12025..12789)
                     /locus_tag="IWO52_001002"
     CDS             complement(12025..12789)
                     /locus_tag="IWO52_001002"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0355284.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(12790..13737)
                     /gene="prmA"
                     /locus_tag="IWO52_001003"
     CDS             complement(12790..13737)
                     /gene="prmA"
                     /locus_tag="IWO52_001003"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0355285.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(13750..14238)
                     /locus_tag="IWO52_001004"
     CDS             complement(13750..14238)
                     /locus_tag="IWO52_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0355286.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(14204..14875)
                     /locus_tag="IWO52_001005"
     CDS             complement(14204..14875)
                     /locus_tag="IWO52_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0355287.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            15013..16299
                     /locus_tag="IWO52_001006"
     CDS             15013..16299
                     /locus_tag="IWO52_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0355288.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            16371..16565
                     /gene="ssrS"
                     /locus_tag="IWO52_001007"
     ncRNA           16371..16565
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWO52_001007"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            16643..16927
                     /locus_tag="IWO52_001008"
     CDS             16643..16927
                     /locus_tag="IWO52_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355289.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            17000..17479
                     /locus_tag="IWO52_001009"
     CDS             17000..17479
                     /locus_tag="IWO52_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0355290.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            17701..17844
                     /locus_tag="IWO52_001010"
     CDS             17701..17844
                     /locus_tag="IWO52_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0355291.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            18422..20005
                     /locus_tag="IWO52_001011"
     CDS             18422..20005
                     /locus_tag="IWO52_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0355292.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20442..21626)
                     /locus_tag="IWO52_001012"
     CDS             complement(20442..21626)
                     /locus_tag="IWO52_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0355293.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(21651..22658)
                     /locus_tag="IWO52_001013"
     CDS             complement(21651..22658)
                     /locus_tag="IWO52_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0355294.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(22830..23189)
                     /locus_tag="IWO52_001014"
     CDS             complement(22830..23189)
                     /locus_tag="IWO52_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355295.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(23161..23532)
                     /locus_tag="IWO52_001015"
     CDS             complement(23161..23532)
                     /locus_tag="IWO52_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0355296.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(23549..23851)
                     /locus_tag="IWO52_001016"
     CDS             complement(23549..23851)
                     /locus_tag="IWO52_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355297.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(23868..24338)
                     /locus_tag="IWO52_001017"
     CDS             complement(23868..24338)
                     /locus_tag="IWO52_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0355298.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(24313..24591)
                     /locus_tag="IWO52_001018"
     CDS             complement(24313..24591)
                     /locus_tag="IWO52_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0355299.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(24588..25637)
                     /locus_tag="IWO52_001019"
     CDS             complement(24588..25637)
                     /locus_tag="IWO52_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0355300.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(25594..26523)
                     /gene="cpaF"
                     /locus_tag="IWO52_001020"
     CDS             complement(25594..26523)
                     /gene="cpaF"
                     /locus_tag="IWO52_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0355301.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            26655..28409
                     /gene="ade"
                     /locus_tag="IWO52_001021"
     CDS             26655..28409
                     /gene="ade"
                     /locus_tag="IWO52_001021"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0355302.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(28507..29169)
                     /gene="trmB"
                     /locus_tag="IWO52_001022"
     CDS             complement(28507..29169)
                     /gene="trmB"
                     /locus_tag="IWO52_001022"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0355303.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(29239..30018)
                     /locus_tag="IWO52_001023"
     CDS             complement(29239..30018)
                     /locus_tag="IWO52_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0355304.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(30225..31436)
                     /locus_tag="IWO52_001024"
     CDS             complement(30225..31436)
                     /locus_tag="IWO52_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355305.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(31429..32166)
                     /locus_tag="IWO52_001025"
     CDS             complement(31429..32166)
                     /locus_tag="IWO52_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355306.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            32304..32738
                     /locus_tag="IWO52_001026"
     CDS             32304..32738
                     /locus_tag="IWO52_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0355307.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            32731..33075
                     /locus_tag="IWO52_001027"
     CDS             32731..33075
                     /locus_tag="IWO52_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0355308.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            33255..34265
                     /locus_tag="IWO52_001028"
     CDS             33255..34265
                     /locus_tag="IWO52_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0355309.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(34334..35278)
                     /locus_tag="IWO52_001029"
     CDS             complement(34334..35278)
                     /locus_tag="IWO52_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0355310.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(35262..37982)
                     /locus_tag="IWO52_001030"
     CDS             complement(35262..37982)
                     /locus_tag="IWO52_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0355311.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(37979..39199)
                     /locus_tag="IWO52_001031"
     CDS             complement(37979..39199)
                     /locus_tag="IWO52_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0355312.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(39480..40325)
                     /locus_tag="IWO52_001032"
     CDS             complement(39480..40325)
                     /locus_tag="IWO52_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0355313.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        12040 a         8440 c         7090 g        13074 t
ORIGIN      
        1 aaaaaaactt caggcaaaaa caaatgtttt tgcctgaagt tttttgtagc tatcattctg
       61 tcacgtttgt ctcactgctg ttcgtacttg aacttcctcc tagcaaggct tcgctgacta
      121 agtgaatgta atgtgtcatc ccttcaggat ttggatggac ttgatcatca taaaaccagt
      181 cactatggtc attactgtag ctgtaccaat caatcagcgt catattgtcg tattttttcg
      241 tcatctgttc aagcatcgca ttcacgtcat tttgccagcg cttcgtcggt actcggacat
      301 tgacccagta tacttttcga tcaccgattg catccatcac actatcaaac tgagcttccg
      361 agaatgagcc attcgttcct agtccaatca gaacagtgtc tttcaacaaa tcttgttcct
      421 ttaatttttc aatgtacggt acactggcat ataattgtcg tccgacatct ccatctacca
      481 ctacttttgg ataaagttct tgtagatccg ttgcagcatc caacataact gaatcaccaa
      541 atgccgttag ttctaatttt tgtgccttat tcccttgtgc cttggacaac tcgtacttat
      601 caaggatcgc ttggtcaacc gaactagtgg tgttttctga attgtttctg gtttcttctg
      661 ctaatttttt actttcagcg atttttgcct gcagttgttt ttgatcggct gttacagcgt
      721 ttttcggtgc cgtaacgaaa ccaacgagtg caattgctac aagtaatgtg cctggaacaa
      781 cccatggttt gctcttactg atgatcggtt tgctgaacca tcctttcatc acttggaatg
      841 tacgaccata atcaaaacgt gctagcggac gctccacata tcgataagaa agttcactga
      901 tgattagaat caatgtgatt tccactaaag tatgcaacaa aacatgatcg gcaatatcag
      961 tcacctttgc ttcataaaaa atcataactg gaaactgata cagataaatg ccatagcttc
     1021 gtttgccaat ccaagtaaat actggatttg tcagccatcg attcaaactt gctccggggt
     1081 gtgctgtcac tgcaactagc actacgcata acagactcac taggagcatt cctccgtaat
     1141 agatgaaatt gtaatgatca tctaaaaaga agaagcaaag aatcagtgcc aaaagcgaga
     1201 ttcctccagc gatattcaga atatttttcg cttgtttcgg gatgtttttc tttaaacgcg
     1261 tacttggcca aacaaacgca agcgcacttc ccaaccatat agaaaacaga cgagtgtcgg
     1321 taccgtaata aacacgggtt ggatcgcctc ctggtgtata caagatcatc atcaagatgg
     1381 cggagaggaa ggatgtacct aacacagtat aaaagatcca ttttctttta cgaacaaata
     1441 ccatcaacaa tacaaacaag atcggccaaa ccaaatagtt ttgaccttct actgctaaag
     1501 accagatatg cgtaaacggt gactcattag caaatcgatc gaaataagat aaaccgttat
     1561 tgatttgcca ccagttgttg acatacagta agctgctgac cacaactcct cgaagattgt
     1621 ttaacagccc tcgctgaaac aaagtgatat aagccgaagc tgtcacaagc ataaaaacaa
     1681 gtgccggata gagtctcttc attcttctga cataaaattg ccaaatattg atttttccat
     1741 tttgttccca ttcctgccgc agcagatccg tgatcaaata accagatacg acgaaaaaaa
     1801 tcggaacgcc caaatagccg cctttcatac tagttggcag aaggtgatag aagataacac
     1861 ccaccacagc taatgaacgg agcccatcaa acccagtgat gtagcggctg ttttttaacc
     1921 ttttttgttt ttccattcaa tattccaact tttcataaac ttttttcatt cgtaatatac
     1981 gggtattaag tgaaaatagc aagtagaatc gtgtcctttc tctactattt ttttctatca
     2041 tcccataaat ttaaaaaaat aaacatgctt tttttcaact ttaagaaaat ttcaaataaa
     2101 aaagcagttt ttctttgctg attctactat ggtagaaaaa aactgcagac aaacgaacgt
     2161 ctgtctacag cagtatttca tgaatttttg tcaaactcga tctaaatgcc atcctcatag
     2221 aaacgaccaa gtatctggac gttttctgca atacttacaa aagcttgtgg atcagaagct
     2281 ttcattgctt ctctaagtag aggcagttcg tatcgtgtaa cgatcgtcat caatactgtc
     2341 tgcttatcat gcttataagc accttctgct tcattgatga tcgtgatccc tctacgcata
     2401 cagttttgga tttcatttac tactttttct ggatgtttcg ttacgatcat tacctgcatc
     2461 ttcttttgtt tagtataaac gacatcagtt acttttccac tgacgaaaat agacaacgta
     2521 ctgtaaaaca tatattgcca gccaaataga attccggcaa caaaaacgat caatgcattg
     2581 aagtagatcg aaatagaacc aatcgacttt cctgtttttt tacgaatcgt gatactcacg
     2641 atatctagac cgcccgatga taggccgttt ctcaacgcta gcccaatacc tagtcccatc
     2701 acagcccccc caaacaacgc acagatgatt ggatcggtag ataggactgt ttccggtaaa
     2761 atatggatac aaagggaggt caaagtgact gtgataaaag tgaaaattgt aaacttcttc
     2821 ccgattttta accacgccaa tacaaacaaa ggtatattca gtaaatacaa agttgttgat
     2881 accggtactt cgaatcctat cagtgatttt gacaaagtcg tcaaaatctg agctagacca
     2941 gtaataccac ttgaataaat atgtcctggc tgataaaaga aattcaatgc gactgatgcg
     3001 atcaacgcat aaacgataga taccgaaagc ttggtcgtgt aatcatgata agcccaattt
     3061 tcgaatgttt ttttcatgga aaagccccgc tttatatgtt atggcttcat tataaagtgt
     3121 ttttcaatta aatcaaggtt gtcaaaagaa gcgtattcga caaatccttt ttaccttttt
     3181 atcggctatt cttctatatt cgttacatca atattcaatt cgaacaattg taaaggcgaa
     3241 accatacttg gtgcatcact catcacatct gctgcttttc cgtttttagg aaaggcaatg
     3301 acttcacgaa tattatcttc tccggctaat aacattgcaa aacggtccaa tcctaatgca
     3361 ataccgccat gtggagggaa tccgtaatct aaagctgtta acaagaaacc aaattgttct
     3421 tccatttgtt cttttgtaaa gcctagagtt tctagtactt tttcttgaag ctcacgagta
     3481 tgaatacgga tagaacctcc gcctaattca taaccgttca agacgatatc ataagcttct
     3541 gcatagactt ttgcagggtc ttcagccaat aacgggatat cttcttcttt tggcatcgtg
     3601 aatggatggt gagcagagac atatcgacct tcttcttctg aaaattcaaa ttgtggccaa
     3661 tcagttaccc ataagaaatt gaatttcgat tcatcaatta gtcctaattc ttttcctaaa
     3721 cgtgtccgga tagcacctaa tgcagcagcg acgatttcac ttttatctgc gccaaacatc
     3781 agcaagtcac ctggttttgc atctgttgct ttaatgattg cttctgttgc ttctcccatg
     3841 aatttagcaa ttggaccttt caatccgtct tcttctactt tcagccatgc aagcccctta
     3901 gctccgaatt gtcctacata ttgacctaac tgatccatgt cttttcgtga gtagcgatct
     3961 gcagcgcctt tagcattcaa tgctttcacg acaccgccgt tttcaagagc catttggaag
     4021 actttgaatt ctacttcttt gactgtatcg cttagatcga tcaattccat atcaaaacgt
     4081 gtatccggtt tgtcgctgcc gtagcgagcc attgcttcat cataggtcat acgaggaaat
     4141 ggcaatgtga cttcgatacc gcgaacttct ttcattacct tcgcaatcaa tccctctgtg
     4201 taagtttgga tttcttctgc tgttaagaac gttgtttcaa tatctacttg cgtaaattca
     4261 ggctgacggt ctccacgtaa atcttcatcg cggaaacaac gaacgatttg ataataacgg
     4321 tcgaatccag cattcatcag caattgttta aacaattgag gagattgcgg caacgcatag
     4381 aaatgtccag catggacacg tgaaggaact aggtagtctc ttgcgccttc tggtgtagat
     4441 ttccctaggt aaggtgtttc gatatcgaga aagtcattat catctaaata atgacgaatc
     4501 gcttttgttg tcgcatttct taatttgatg tttttagtca tttttggacg acgcagatcc
     4561 aaataacgat atttcaaacg tatttcgtca ctgattgctt gatcatcttc aatttgaaat
     4621 ggcggggttt ttgcttgatt caaaatcacg atttcacttg caatcacttc gaattcacca
     4681 gtggccattt taggatttac tgcattttct ccgcgtcttc taacttcccc agtcacttcg
     4741 atcacgtatt cactgcgaca tttatccgcg atttcccatg cttctttgtt gatttcagga
     4801 ttaaagacga tttgaacgat tccttcacga tcacgtaggt cgataaagat tactccccca
     4861 aggtctcgtc ttttttgtac ccatcctttt aatgtaactg tttgttctaa ttgttgttcg
     4921 gaaactaatc cgcaatagat cgttctttgt gccatttatt tttttcctcc tactcgaata
     4981 aagtcatttc atcatagact tcatcaaaat gttcatatat ctcttctaat ggaaaacttt
     5041 tttcttttcg gcttgccatt gctttgacat taatcgtttg actagcaagc tcatcttcac
     5101 cgatcaccaa tactaatttg gcattcgctt tatctgctgt cttaaattgc gcttttgctt
     5161 tacggttcat gaagtcgcgg tcagctgaaa aaccaaagtt tcgaatcgct tggaccactt
     5221 tcaatgcttc gatatttgtt tcgtctccga gtgcgaccac gtaagcatcc aactcattaa
     5281 atgcagggat aactacttcc tctgcctcca tagtcaagag taatcgctcg atacccattg
     5341 caaatccgaa gccaggtgtt gaaggaccac ctagttcttc cactagattg tcataacgtc
     5401 cgcctgcgca aatcgttgct tgtgccccca tttttggtgc gtcactcatg atttcaaaaa
     5461 tcgtatgtgt gtaataatct agtccgcgaa ccatattact gtcgatttca taaggaacag
     5521 ccaatgcatc cagcatggaa acaacggtat caaaatgttt ttgagcaggc tcgcttagat
     5581 aatctaagat agatggagct tctgccacaa atttttgatc tcgtttgtct ttactgtcca
     5641 atacgcgtaa tggattttca tgaaggcggc gtttagagtc ttcactcagc tctgattcaa
     5701 aaggtaaaag atagtcgatc aatgcttgac gataggcttg acgcgtttct ttatcaccta
     5761 atgaattgat gactagccgg atttgcgtga tccccaactg tttgaagaaa tcaagtgcca
     5821 tcaccatgct ctccacatca gttgcagggt tttctgatcc aaatgcttct actccgattt
     5881 gatggaattg acgtaaccgt cctttttgag ggcgttcgta gcgaaacatt ggtcccatat
     5941 aaaaagtttt gaacggtttc gcatactctg ggccgaacaa tttatgctca acaaaagaac
     6001 gaacgattgg tgctgtacct tctggtcgaa gcgtcacatg gcgttcccct ttgtcataaa
     6061 aatcatacat ctcttttgaa acgatatccg ttgtatcccc tacacttcgg gatatcactt
     6121 catagtgttc aaaaatcggt gtacgcattt cattatactg ataatctctg aataataaac
     6181 gtgctgtttc ttcgacaaac tgccattttt ctgattcgcc tggcaaaata tcatttgttc
     6241 cttttggtct ttggaaactc atagttttct ctccctttct tagctatgta aataaaaaaa
     6301 tcacccttgt tcatcatgaa caagggtgaa tgaaattcat acacggtacc acctaatttt
     6361 gaaagatttc tctttcctct tacgaataac gctcgtacgc gggacggctt ttcactcgtc
     6421 catctccaga gtgtctttag gctaatgaaa ctttagatca ttttcagcct cgatgatcct
     6481 ctcttgtaaa gtttcaaaaa cttactcgct ctttcattga cattcgatat tacttataac
     6541 ctactaaaaa ccagtctcaa agtcaagaga tatctgacta tttcccctca caaaaacgtt
     6601 ttagttcagc tttttggtat tttgttttag actagacaaa aaggaggaat cagctatggc
     6661 agaaataaga atcggacatt cacaagtcta cgcagaacag cttggattag gagcaaatgc
     6721 cgttggagga cataatcttt ttgacggttt ggaagatgag acaggaaaac aagtagttcg
     6781 tacagctttg aatagcggga tcaatttgat cgataccgct tatgcttacg gaaacggccg
     6841 ttccgaagaa ctgattggcg aggtactgaa ggaaaaagaa tatgatcgct cacgtgtcgt
     6901 catcgctaca aaagctgctc atgtaccaaa taaagggcga acatttgata attctcctga
     6961 gtttctcaaa caatcggtgg aagatgcact aaaacgtctg caaactgatt acatcgacat
     7021 tttttatatc cactttccgg atgaaagcac accaaaaaat gaatcagttg ctacattgca
     7081 cgaattaaaa gaagcaggaa aaatccgagc agtcggtgtg tcaaacttca cgttagaaca
     7141 attgaaagaa gcgaatgcag atggatatgt cgatgtagta gaagataaat acagtttgat
     7201 ccatcggcag gctgaaaaag aattattccc ttatcttgaa aaaaacaaaa tcagttttgt
     7261 tccctatttt cctcttgctt ccggtttgct gacaggaaaa tatgaacttg gtgaggaaaa
     7321 acaatttggt gaaggagatc ctcgaaaaag aaatccagat ttccaaggag aacgtttcag
     7381 agaaatttta accgctgtcg atgtacttcg ccccattgct aaaagatacc aagccactcc
     7441 tgcgcaactt gttttagctt ggtacatgaa gaatccacga gtgtctgtag ttatacctgg
     7501 agctaagcgt ccagagcaag tatctgacaa tgtccaagct ttagatttgc atttatccaa
     7561 tgaagattat caaaccattg atgaagcatt tcgtggattt tgatcaattg accttatcaa
     7621 tttatagtaa tatgaaaaaa ggaagatcag cactgtttga tcttcctttt tttcgataag
     7681 tttatgctaa tctaattctt gtaaagtagc gctcggctat tcggataagt cggaaactga
     7741 ttgaatggaa tcgtccaatc ttgttcaggt acagagaact caaacttatt tactagctga
     7801 tcggaaaaca cacgtagact atgaagagtg atccattctc ctgcacagcg atgcatttgt
     7861 cgaaaatttc ccccgccttg tgcaatcatc tcatattctt ctttatagga aatatctttt
     7921 gctttcccta cataccgttt gatcataaag gaatctggcg cttcaactgt tcgttcatca
     7981 tggtctgttc catataaatc taaaataacc caactgcctt ctggtatacg ataaccatct
     8041 acttctactt ctttcagcga aatcgcaggt agcattggga agaaaggata atagcgtctc
     8101 atttcttgaa taaacggatc ttgtaacgtc gaaaagtcat tttttaattg ctgatacaaa
     8161 tctggtctac tgaaaagtgc atgtcccatc aatgccgccc acacagttag agcaaccgtt
     8221 ggacggatga tgttcaataa ttcaacagct gccacttcca gaggtaaaag ctgaccatct
     8281 aaatctgtgg cattggcaaa agcatataat gccacatttt cttttccagc cacaggattt
     8341 tgtcgagctt cttcaatcaa gccttgcgcc catttttctg attttttgcg attctcgaca
     8401 ccttttatgt gatcaatcgg agaagtaaac gttccgctga tcatagagat ttgatattct
     8461 gctaattttt cgacctcatc tttagaaatc gctgataaat tgatacctgc ccactcgcaa
     8521 atagatgtaa agaaaaccat cttacttaaa tcaaatagtt caaattgacc atgctgtgct
     8581 tcaagtgctt gtgtcagatt cttatctaag atacgatgat aatcttccat tctttccggt
     8641 gtcattagat ccataaaaat atttttccga tggtgatgag cagctccatc caaagtctgt
     8701 actccacctt gaccaaaaag tgtcttcaat actaattttg gcatagcacc ttttcgtttg
     8761 aaattccttg gatcataaaa tacttttgct gctgatgatc cgtagatcgt gatagcttct
     8821 ttattgaaaa tctttgcttt gacgacggga gcgtccgctt catggcgtaa ctcctccaac
     8881 atgttgtatc ctttttgata caattttttt aaatctgtga ttttgatatc tactactggt
     8941 acttctttca tcttcagcaa cctcctataa aaaataactt gtataattta agcttaactt
     9001 tttttccgac tgctgacaaa agaagcgctt tttgcagaca aaaaagccac ttaagttaga
     9061 gtttatcagt ctaacttaag cgacgtgttt catagttttt actagtgaaa accagattac
     9121 tttatttgtt tttagaatca ataatgatcg ttacaggtcc atcattgacg agagaaaccg
     9181 acatatctgc tccgaattgt ccggtagcga ctgtgatgcc gcgacttctg attccttcat
     9241 tgaatgattc gtataaagga atagctgttt ctggtcgtgc ggcttccaca aagcttggtc
     9301 gatttccttt tttcgtatcc gcaaacagtg tgaattgaga gatacttaaa atttgtcctc
     9361 ccacatcttc tacactaata ttcatttttc cttgttcatc ttcaaaaata cgcatttgtg
     9421 caattttttt taccagatag tccacatctt tttgcgtatc ggtttcatga atgcctaata
     9481 aaacaagtag gccgtagcca atttttccaa tttcttgctg atcgactgac acagcagctt
     9541 tacttacccg ttgaacaact gctctcatat ctgtcctcct atccattcgt ccggcgtaca
     9601 ctgtagacat caggtactgc tttgatttta tccacgatac tctttaggtg ggataaattc
     9661 tggataccaa ccgtcaaatg gatcgtcgcc attttatctt tattcgtccg tgcttcaacg
     9721 ctgttgagat ttttcgttag cgcattgact gtttgaagta catcattcaa taagcctgaa
     9781 cgattatagc cgtaaatttc caaatctgca tcatactctt ttcgtgtatt cgatgtatct
     9841 tcccattcga cttcaatcaa acgttctgct acatttggtt tgtcaggctg aacattcgga
     9901 caatctcgac gatgaatgga tatccctctg cctttagtga tataaccaac gatatcatca
     9961 ccaggaatcg gattacagca gcgactgata cgaattagta agttttctac cccttgaatg
    10021 acaacgccac cttcatgacg tactttcatt ttgtcaggtt cttttttcgg ctgattcatg
    10081 atttcttctg cttcttgctt ttgctgctcg attttttgtt ctttacgttc tttttcagtc
    10141 agacgattgg ccatcgtcaa ggggctaact tctccatacc ctacagctgc atagagatca
    10201 tcttctgttt gataattaaa acgatcgagt gcttcttgca gtttgttctt cgtcaaaata
    10261 tctttaggcg taaatcctag atctgtaata catttgacca cggattcgtg gcctttgatc
    10321 acattttctt ctcgatcttg ggctttgaag aaacgtttga tcttatttct tgctttgcta
    10381 gtagcaacta atttcaacca gtcgcgactt gggccaaatg aatttggaga agtcatgatc
    10441 tcaatgatat ctccgttttt caatttgtaa tcaagctgca ccattttgcc atttactttt
    10501 gcaccagtgg ttttgttacc gatatctgta tggatactgt atgcaaagtc caatggtccg
    10561 gatccttttg gtaactcagt aacgtctcct tttggtgtga agacgtagac tttatcacta
    10621 aagatatctc cttttacgcc ttccataaat tcagaagcat catagctttc gtcttgaagt
    10681 tcgagtatct catggaacca gcttaattgt ttcgtcatac catctggttc tactttttca
    10741 ttttttcctt ctttataggc ccagtgtgca gcaaccccga attcagcgat ttcatgcatt
    10801 tcctgtgttc ggatttgaat ttctaccgga tttcctgccg gaccgatcac ggtagtatgc
    10861 aaagattggt acatattagc ttttggcatg gcaatataat ccttaaatct gccgggcatt
    10921 ggtttccatt ttgtgtggat tgctcctaat acagcataac aatccttgat tgaatctacg
    10981 atcactcgaa tagctagcaa gtcataaatt tcgttgaact gctttttctg atctttcatt
    11041 ttacgataga ttgaataaat gtgttttggc cgtccataga tttctgcaaa aatccccagc
    11101 tcttctgtcg ctattcgaat atcttcaaca gtaccactta cgtatttttc acgttcttct
    11161 ctcttcgtct gcatcaagtg gacgatacgg taatactgtt ttggatttag ataacgaagt
    11221 gctgtatctt ctaattccca tttgatccgg ctgatcccta gacgatgtgc aagaggcgca
    11281 tagatttcta aagtttcctg agcgatcctt ctttgcttat cttctcgcag gtgcttcaac
    11341 gtacgcatgt tatgtagtct atcagctaat ttgaccatga taactcgtaa atcttgagcc
    11401 attgctagca gcatctttcg gtggttttct gctagctgct cttcgtggga tttatatttt
    11461 attttcccta atttggttac gccgtccact aacatagcaa cgtcatcgcc aaattcttct
    11521 ctcagatctt ctaaagtgat ttctgtatct tcaacaacat catgtaagaa gcctgtagcc
    11581 actgtatggg gatccatttt taaatctgct aatatacctg cgacttggat aggatgaata
    11641 atgtagggtt cgcctgattt tctaaactgt ccgtcatgtg cagcagtcgc gtattcgcat
    11701 gctttctcaa caaatgctac atgttcggga cccatgtatt gactaacgag ctttatgaca
    11761 ccgggtcccg ttaaaatttc ttcttttgtc acaggaatcg atcccccttt cttttttcgg
    11821 taaaacggga atcgggacaa atatctgttt gataattgtc ccaaaatccg tgcagttata
    11881 cgttttttta cggaaatgtt cgctttttaa aagctttttc aaacgatttt tcgtatccat
    11941 tgtataaata attgtttcta ttatacaaat tgttgactat tttttcaatt ctttctgtcg
    12001 atactctttt attaatgaag aacgttagct gaaagaagga ttcattaatt cccattgata
    12061 gctagcagca cttaataaat aaagaggtgc tgtttctgta cgaaggattc ttggtcctaa
    12121 tccacaaagt atccctttgt gttctacaaa ataatcgact tcatcgcggg ataatccacc
    12181 ttctggtcca aagacagcta agattcgact atctggtttt gtttcactaa gaattttggc
    12241 tgcatttgat ttttcgcctt gctttgctga ttcctcataa gcaaccaaga ccacgtcata
    12301 atcatctagc cgctcaaata aatcttgttt cgtttccaat aactctactg atggcacgat
    12361 ctgccgatgg gattgttccg ctgcctcttg agcaatcttt tcaagccgtt gttgtcgttt
    12421 catacgtttt tttgcatccc atttaacgac agaggctgat gccgggaaac caagaaaagc
    12481 ttgcgctcct aattcagtgg atttctgaac gatccaatcc agcttttccc ctttaggata
    12541 gccactcgct atggtaatcg aacttggcag ttccttttga ttttcttctt ttttaatttc
    12601 tttcagataa acagcctctt cagtaatctc cgtgatttct gcaacaatgg ctatttgatc
    12661 attgaatgcc aaaaaaactt catcctcggg ttccatcctc ataacccgta caatgtggtg
    12721 atagttttcc ccttctaaaa caaaatagtc ttttgattca tatgattctt ttaaaaaata
    12781 ccgctgcatc tattccacct ctggtttttt caaaataatt gcataccagt ctttttgctg
    12841 gaaaatttga tccacgataa acccttgttc taacatggca tccagaacca tttgcttctt
    12901 ctcttcaatg attcctgaaa cgatcagcgt gcctgtttgc ttcagtaaac gccaagcatc
    12961 tgggatcatc aataagatga tatctgctaa aatgttcgca acgattacat cactttcaat
    13021 ctcgattcct gtcaaaagat cgttagctga tacatggaca tctttggcta tttcattcat
    13081 atccatattt tctttcgccg aacgaacggc tacttcgtct aaatcaaagg catagacatc
    13141 ttttgcacca agatattttg ctgcaatact tagcacgccc gacccagttc ccacatctag
    13201 caatgtttca ccaccacgca agactgtttc tagtgctgtc aatgtcaagg aagtcgtggg
    13261 atgagtgcct gtgccaaatg ccataccagg atctagtgtg ataatttttt ctagaggatc
    13321 ttctgtctgg tagttttccc agcttggcgc aaccgtcagc attcgcgtaa tacgcaccgg
    13381 atgatagtat ttcttccagg ctgttgccca gttgctttcc tctacttcac taactgttac
    13441 tttatttttt ccaatagcta gaccaaactc aggtaattgt gtgatacgat ctttgataaa
    13501 aggcaagatt tccggtaaga aagtcgtttc cggaaaataa gccatgacta gtgcgccttc
    13561 tttcagcgat gtaaaatcat ttttatctaa aatctcacca tattgatccg actgaaaatg
    13621 ttcaacatct aaagaatctt caatcgcgac accactcgcc cctgcctcca tcaaaatatt
    13681 agcaactgct tcgactgctt cactttcagt ttctactttt acttcattcc atttcatatc
    13741 tgtcactcct taataacttg ggtacggtaa ataagtatct gcttcatttt gctgttttac
    13801 ttctactaat tctgctagtt gttgattatc ccagtgaagc tcgaatacag cttcgtcatc
    13861 gtcttcatcc aaaaaatcaa gcaaatcgct ttgcccttga gctaatactt catttagata
    13921 ttccactaaa gaatcagcta ctgattttgc caaccctttt tttccttcat aagggattac
    13981 tgctagataa ttgcgttcat caaaaacaga tttctgagga ttaaaaagaa gtacgccgtc
    14041 ttcaaattcg ataattggtt cttgcgaaac gatcccttct gcatcatcaa tctcaaattg
    14101 tgctttattt tctgcaaaca agcgaacaac gatctcgatc gtatgatttt ttgtatccca
    14161 atctaaagct acatcatagt ctgtcacttg tttttcaatt atttcatcta aatatgttaa
    14221 tagatccttc tttttcattt tctttcctcc aaccccaatc tttctcgatt tggttttttc
    14281 tttgtaaaga gatataagga tttcccgaca caacaaaacg caacggcttt tctgtccata
    14341 cacctttgtt tgggataccg attctaggca aagcattgat cttttttggt gtcttttttt
    14401 tctcaaaaac taagcgtaac gaactatctg ctatagactg accataaagt tcttgaggaa
    14461 ttgctaatgc ttccactaat tttcccggac cgttgctgat atctggacca gtttttccgc
    14521 cacgattttt actcatttga tcaatcattg cggcgggttc gattgcacgt atcattactc
    14581 cttgaggaat cccttctggc tgagtaatga tgttcaaaat ccgatgagta tgcatcgtgt
    14641 aaaggtagat cgtacctggt ttttcataca ttgcccgcac acgcggtgtt cttcttaaac
    14701 catagctatg ggcggcctcg tcttctggac ctaggtatgc ttccgcgtct acaatatatc
    14761 cagctaatct tccttctggt gtgtcatgct ccaaatacat ccccaataaa tcccgtgcca
    14821 cttctggcgt cgttttattc caaaaaaatt catctatctt ttgtttatcc gtcatttttc
    14881 tcacctactt tatcatacac aatattagtt ggataagcta tgaaaacaaa acaacagaag
    14941 gctgttcttt ctaggcatct tgctaaatat gatataatta agggacgtaa aaacaagaag
    15001 gaggaaaatc ctatgcaaca gccattggct tatcgcatga gacctcgaac gatcgatgaa
    15061 gtcgttggtc agcagcatct agtcggggaa ggaaaaatca tacgccgcat ggtcgatgct
    15121 cgcatgctct cttccatgat tctatatgga cctcctggta ctggaaaaac gagtatcgca
    15181 agtgcgatcg ccggctctac taattatgca tttcgcatgc taaacgccgc tacagattca
    15241 aaaaaggatc tacaagttgt agcggaagaa gcaaaaatga gtggtacagt gatcctctta
    15301 ttggatgaag tacaccgatt agataaaaca aaacaggatt ttctgcttcc tcatttagaa
    15361 agtggccgaa tcatcttgat cggagcgact actgagaatc cttatattac catcaatcca
    15421 gcgatccgca gccgtacaca gatctttgaa gtcaaaccat taacagaaca agacatccag
    15481 ttggcagtcg aacatgcttt aagggataaa gaacgcggcc ttggacaaca agcaatccag
    15541 ttagacgaag atgctcttct tcacctttcg cgtgcaacga acggcgattt gcggagtgca
    15601 ttgaatgggc tggaactggc tacactatcg actccttcag ataaagaagg aaggattcat
    15661 cttacactgt cgatcattga agaatgtgtc caaagaaaag ctcttaccca tgacaaaaat
    15721 ggagatgctc attatgacgt tatttcggct tttcaaaaat cgattcgtgg aagtgatgtg
    15781 gatgctgcac ttcattatct cgcccgttta gttgaggcag gtgatctggc aagtatttgc
    15841 cggcgtctga tggttatcgg ttatgaagat ataggtttag gtaatcctgc tgctgcggct
    15901 cgtacagtga acgctgtatt agctgctgaa aaattgggat tgcctgaggc acgtatccca
    15961 ctagcagacg tcgtcgtcga tctttgcttg tcgccaaaat ccaattctgc ctacatggca
    16021 ttagatgcag cacttgctga tattcgagag ggcaaagctg gagatgttcc tgatcatttg
    16081 agagatagcc attacaaagg tgcgaaatca ctcaaccgtg gcgtgggtta tcagtatcct
    16141 catcattttg atcaggcttg ggtcaatcag caatatttac cagataaact gaagaatgct
    16201 cagtattatc agccaaaaga tacagggaaa tatgaacagg ctctgggtca acagtattac
    16261 cgtataaaag agtggaaaaa gcatcctcca aaaaactaga caagaaaacg gttctgtttc
    16321 tgcgagctct ctcattgctc tttttaaatt catatgctac tatagaggtg ggaaatctgt
    16381 gatgttcgaa ttgtcctctt tgtgtgttga ccgaacattt ttattgatat cttgggaccc
    16441 gtcttgtgtc actgctggta acatgcctta tcatttggta gttcgaagct acgaccatgg
    16501 gacccacctg cttgaattcg cgggatcaat actacaggac aaacaacggc attaacggag
    16561 attaccatct tttaaagaat aaagcaattt gctcttggca aattgctttt tctatttgtt
    16621 gcttattttg gaggatttgt tcatgattag gattttaatg attatcgcca ctttagtatt
    16681 attattcgtt agctattatt tatttaataa acaggatatc ttttttgtat tgatcaacaa
    16741 aaatgataaa aatcaaggat tcctacaatt ttacggtgct gcatatgctg ttttaggcgt
    16801 aatgggtatt ttaacagctt ttttcaatca acgctttatt gccttggtct ttttactgat
    16861 cgtaattatt gtttctgcca ccttcagtat ccgatttgca aaaaaaattg ctgaaccaaa
    16921 gcagtaaata attctctttt tccctaaagt gttctacaat aaaagagtac aatacgattg
    16981 acttgaggag tgaatgctta tgttacaaca atacaaaaaa atcatggttg ccgtagatgg
    17041 ttctgatgaa gctgaattag cttttaaaaa ggcggtcaat gtagctatcc gaaataacgg
    17101 agaacttcta ttagcacatg tcattgacac acgttctttc caaaccgttt catcctttga
    17161 tggaatgctt gcagaacaag ctactgaaat ggcaaagcaa acattggcag attatgaaag
    17221 caatgcgaag aaagctggct tgaataatgt aacctctgtc gtagaatatg gctcaccaaa
    17281 acaaattatt gccagagaaa tcccagaaga taatcaagta gatttgatta tgttaggagc
    17341 cacaggattg aatgctgtag aacgtttatt tattggttct gtttctgaat atgttattag
    17401 aaacgcagct tgcgatgtgc ttgttgtccg aactgattta gaaaaccaat tgccagctaa
    17461 agaccacacc aaagcttaac acgtagacga cctgccaact atcggcaggt ttttttgcac
    17521 aaaaaaccgt gataaaaaca aaagacgcgg caagcactca tcggtcttta taatgtttaa
    17581 aataaaggga aaacagtcaa tgagttcaag taaaataaaa aaagtgaaca ccagggcgat
    17641 tagtcgccct ggtgtggttt aaaacgacct aagcatgcca aagcttattg gttcgtcaat
    17701 gtggaagttt ccgatgaatc tgatacgaaa aaatatgaag tatcgatggc ggataccgat
    17761 tttatcgcca aactaaaatt ttatttcggg ttcgggtacc gcgagaaggc aagtgctaca
    17821 ttcggtaaat cattcaagaa ttgatgaaag gctaccgctt gccattgcaa ccacaacgat
    17881 actcctgaac gcgatgttgg tgagatttcc aaagagaaga gctgtttaat gcaacggtat
    17941 taagcaaatg gcaaaaggaa gtcatgtaga caaagttttt tatcaaacac catatgattt
    18001 cgcctgaacg ctggtttagc tcagaataca atgatgtggt ccacgagaca ttaccctcag
    18061 tcagataaaa aaagctctct gacagaatta cacatcgata aatattaagt aaatgtcagc
    18121 gctagttgaa ctttctgaca ctttcaggta cactggaaaa gacaactaca cgaaggaggg
    18181 aaaatactat gatgcgcatg actaaaataa caagtaatga aaattaccaa cgataccata
    18241 tcgcatttag ccatgcaatt ggttttacag ttgctgttga cgggacaagt ttgtccaaaa
    18301 cagttgaatt cacgtaaaat caatggccat ctcaaggtat ttttctcatg ataatctgag
    18361 tttaaataac acaggccagg gataaccatg gcctttttga gtggtctcaa ggagagagat
    18421 catgatcata caagcaattt ctattaaaaa aacaatcggc gggaatccat tatttgagga
    18481 tttatcctgt caaattgaag ctggtgaaaa aattgcctta gtcggtatca acggaactgg
    18541 caaatcaacc tttttacaaa tcttgaccgg cagagaaggt gtcgacagcg gtgttatcag
    18601 tcgcaaaaaa ggcttaaaga tcgggacggt cgaacaagaa cttacagttt ccgaagcgac
    18661 cgtcaaccac tacttgctcc attctgccgc tgagatacag gacctcaaac agcaaatgag
    18721 tcgctacgaa gcattgatga cagaaccaga tacaaactta gagaagtgtc tggcccgtta
    18781 cggtgagctg cagcatcgct ttgaagaact aggcggttac gtattagaag accggattgc
    18841 agcgatcttg caaggcttag ggattccaca ccgtcaggaa gcacggttaa cagaacttag
    18901 cggaggcgaa cgagttaaag tcgctttagc caaaatttta gcagccgatg ccgatttact
    18961 tttactcgat gaaccgacca atcatttaga tcttaacagt attcgctggt tggaaaacta
    19021 tttacagaat acaaaaaaat cggttctggt cgtttctcat gatcggcaat ttctcgatca
    19081 agtcaccaca aaaacatggg aactggaaga aggtgcattg attgagtatc ccggaaacta
    19141 cagccgtttt cgagtcttga aagaagctcg cttggctgag ctgacaaaaa attacgaact
    19201 gcagcaaaaa gaagtccagc ggttgaaagt catgattcgt cgttttcgtc agtgggcaca
    19261 tgaaggagac aatgagtcct tcttcaaaaa agccaaagaa ctggaacgcc gcctggcgaa
    19321 actgacactc gtcaaaccgc cacctccccc gaaaaatcgg ctgcaaagct tgtctaatgg
    19381 tggaaaatct ggcaaagaag ttttcatcat tcaaaacttg caccagcagt acgctgatca
    19441 agtactattc aaagacagca gttttgcagt ctatcgtgga gatcatttag ccatcatcgg
    19501 cgacaatggt gcaggaaaat caaccttact caaattatta ctcggtgagg aaaagcctta
    19561 cggtggcacc atcaaattag gtagtagctt gcaaatcggt tatttgccgc agcagctgca
    19621 gtttgccgat cccgatagcc gtttgctggc ttacgccatc acattgactg gaaacgaaga
    19681 agtcgctcga cggcaattag ccaaaagtgg cttttatcaa agcgatgtgg gtaaacggat
    19741 caaagattta tccggtggtg aaaaaatccg tttggcgttg ctgaaactct ttttagaaaa
    19801 aatcaacgtc ctgattttgg atgaaccaac caatcacctc gacagctatg ctcgagaaga
    19861 aattgaagaa atgctgcaag actatcaagg aacgctccta gcagtcagcc atgaccgcta
    19921 ctttttgcag caacacttcc aagaagcact cgttattgat caggagcaga tcagccgcca
    19981 gccgctcggc caactgattg actaggacgt tttttcgcaa caaaaaactt gattttcata
    20041 cctgtgcttt cacgattcag gcaaagtaca ggtggaaaat caagttttta ttattcgatc
    20101 aatgaatgtt tcggatcggt tttctagcta aacataaagc gaagaataaa gtcgacagcc
    20161 cacgaggctg atctaaaaat gttttttctt tttacatatg taatgtccct tataaagatg
    20221 aatgaaaata aaatattggg attttcctga aaatcaataa ataaaatgcc agtcttttcc
    20281 cttttgacca cttttccaag caaaattccc taagaagatc ctgtaaaaaa gaagggaaat
    20341 tgaagttaaa ttaccatttt gcggtcttct ccgcaaaaaa aacacatgca agaattccct
    20401 cttgcatgcg tttcatcttt tatcctaacg aatgctttgg cttagccgcg cagacgctcc
    20461 acatcacggg caatcattag ttcctcatct gttgggatca ctaatactct tacttttgat
    20521 tcttttgtag agatatctac ttcttcacca cgcaattcat tcaacgctgg atcgatttca
    20581 caaccaaacc aagacatacc tttgatgatt tcactacgta catgtccgtc attctcgccg
    20641 ataccagcag taaagacaat tgcatcaaca ccattcatcg ttgtcacata accgccgatg
    20701 tattttcgga tgcggtccac gaaaatgtca taagctaaac gaatatgttc tttttcatag
    20761 ttcttttcaa gatcacgcat atcactagac aatcctgtta ggcctagcag accagatttc
    20821 ttgttcaaaa cattgatcat ttcatcaata tcaatctcta atttttccat taaatatggg
    20881 agaacagctg gatcgatatc acctgaacgt gtacccattg tcaccccagc taatggtgtg
    20941 aatcccatag aagtatccat ggatttacct ccatcgactg ctgtgatcga tacaccattt
    21001 cctaagtggc aagtgatgat ttttagttct tcgatcggtt tgttcaataa ttttgccgca
    21061 cgttccgaca cataacgatg acttgtgcca tggaagccat attttcttac tttgaaatct
    21121 tcataataag acaccggtaa gctatataaa taattttgct ttggcattgt tgagtggaaa
    21181 gatgtatcga atacagccac actcaaaatg tcaggtaaaa tctttttaaa cgctttgatc
    21241 cccattaagt ttgcaggatt atgcaaagga gcaaattctg ctaattcagc aatgcggtcc
    21301 attactttct catcgatcac tactgaatcc ccataagttt ctcctccgtg aacaacacgg
    21361 tgccctactc cggtgatttc atcataagag cttaaaattt gcagttcaat caactgatct
    21421 aacagcattt ttactgctac ttcgtggttt tcgatatcca tgatttgttc gaatttttga
    21481 ttatcgccgt acttgatggt aaaaatagaa tcttgtaaac cgatacgttc gacgatccct
    21541 ttcgcaacta cggtttcttc tggcatttga tataattgcc atttcaaact tgaactccca
    21601 gcattgattg caattgtttt agacatatga ctctccttct cgttgaatga ttacagagca
    21661 gatgatttcc agtttttaaa ctcgttgaaa aattccgtca ccttctctgg ttcttttaat
    21721 gaagcaagtt tcacaagcaa gacttctttt acttgttgtg cttgtggtcc ttttttctgt
    21781 aagatcaaaa tacttttttg cgattgttta ttacggaaca attcatctgg caattgaatc
    21841 attccttgaa gataaccttc ttctttaaac cattttttga tttcttcact ttgatccgtt
    21901 tctaaaaatc cactcggcat caaaaacaaa ccaaaaccat ccggcttcac atatttcatt
    21961 gattgctcta gcaataaatg atgtgcataa ctatggcctt cttcggtact tgttaaaaac
    22021 tcttttgctt tttcgtcatt cggataataa ccgattggca aatcgctgat tgcaaaatcg
    22081 acaggatcta ttaataattc ctgcaacccg tcttgatgaa agtattgaac atttgcttgt
    22141 gtcaaatcac ttgtagaagc tgcgacagcc aacaatgtat catcaatatc cacaccaatt
    22201 ccttctgttt ggtaccccgc attcgatagg tttaaaagaa ctgttaataa caagttgccc
    22261 attcctgccg caatatccag aatcttaacc ggtgcttttt tatttgtgta tagttgttca
    22321 ataagaaaaa caaataaaaa accgatacta tcaggagtga gttgatggtt cgcctgcaaa
    22381 tgttctgtct ggctgccctt caataaaagt aattgagaca gtctgcgcca ttcttctggc
    22441 tcgaaagaaa gtttcttcaa ctcttcataa agagctgtga tccgttgcgt tgtctctttt
    22501 gtcggcactc catccaccac acgtacttga taatcatcta ttaaattttc tgcattttcg
    22561 acataggctt ctaaaaagga agttcccaat gcattctgta aaagctgaat agcttccagg
    22621 ttttgattaa acccttgttc cattttttct ggtaacatgg tgcacctcat ttctagatat
    22681 attataacaa ttttttcacc tgtttaattc tatcgaacaa ttgacaaaat ttcaatcgtt
    22741 ttcactgttt tttatttgtg aaacggcaaa cattttttta ttaaaaacag tgttataaca
    22801 gaaaagaggg cgttttcacg aacattccat cattctgtac tagtatctgt atcatttttt
    22861 acttcttctg tgaaccgaaa gataaacggt gagacttcca cctctaagaa tagggactgt
    22921 tgtacccact gatatgttac agatccagtt gtgtagtaaa aaactccttc cgaactttcg
    22981 gcaagcgtag actgttccga taaagccatc tgcttcatta tttttgctat ataataattt
    23041 ttgattcgta tattaagatc cattgacaaa cggtagcttt ctaatgctgc catcaataaa
    23101 aacgtacata aaagaaataa aagcaagaca gaaaataaaa tagagccctt ataacatttt
    23161 ctactgatcg ttttttgtcc atatggcata tccatcttct ccattttcaa agtgtacata
    23221 aaaagaaatt gtgtgttctt tttccaataa ttgaagctca gtgatacttg ttaacatagg
    23281 ttgatgtccg ccggaagttc ttcttttacg tatcattgcc tgatatcttt cgatgatata
    23341 ctgtttatcc tcgatcgtgt agtgcagttg ctgatttttc acagagacat tgtgactgct
    23401 cttgagttct tcttctaatt gtgctaaaaa tattagccac tctttttgat ctttacgctg
    23461 caaaaattca cttacttgga tggtttgctg aatcaaaaga ttgaatgtga gcaatagact
    23521 gctcaatatc atcagtccaa ttagacattc aatcagcgta aatgctggaa ttacttgttt
    23581 tttcaatact gatcgcttct ttctccatat tgacaaacac tttttcgatt actccattat
    23641 tatcgacaat ttggatatcg tatgggatca tttcttccct tcgctctggt cttttgtgat
    23701 acatccgctg cgtcttgata tcttcataaa tcattcgaat aaatagcagt tcctccccac
    23761 ttttcttttc tgcctgaagc aaaaacagct ggccaaagct cactaagtag acacagacag
    23821 aagcgatcat aagcgctgta agggactcca acaaaacaaa tcctgtttta ttcaattctt
    23881 tttgtatagc gaccactccc tatctggaat tgatacgtaa tcgtccgctc tttttcttcc
    23941 caataaaatt gataagcttt tagtgaactt tcattcccgg ttctagcagc aaaagtaata
    24001 gaagcatggc gtttcaaggt aatctcatca ggaatatcta atacttccca atttatttta
    24061 tctggattcg gaacatgaaa aatgatttga ttctcttcac tattccaata aaagcctgtt
    24121 tggatctgat tcacaatagc gattttttgc gtagaataga ttcgtttttc aaattggccg
    24181 aaaaattgat agactgccaa ttcattcttc catgcagaaa atgcgagaac tggaaaagaa
    24241 aggatcgttg taagtaataa aatcagtaag agtgattcaa acaacgtata tcctgatgct
    24301 aaaaccttac cttcaaggcc gctgatagat ttctgcatgt tctcgtttga tatagccttc
    24361 attcaacaat tcctccacag taggaactct tccattattt tgaagcgcat agagctcttt
    24421 ttgtgtgtct accaactgga tgatcgcttc acgactttct tggtctacat gattcctata
    24481 acgcgacaaa ttagggacga acaacaacac taatacactg atgatcagta agacgattag
    24541 catttcgatc aatgtaaaac cgctataata agatattttt ctctttttca tggcaaaacc
    24601 tcctctatgc ttccgtaaac aggtaataaa acagcggcat aaactaataa aatcaaagcc
    24661 gctacgccca aaaaaatgat tggctgaata aaatgcatcc agtgatgaag acgtttgaaa
    24721 agctgttttc ttgtccattc gctgtaaaaa atcagttctt tacctaaact tccctttgcc
    24781 tctccctgta aaacaatttt actgaattct tctgatagaa aacgataatt ctggaactgc
    24841 tcagccaatg acatacctgc ctccaatcct tgaatcaact tagaggcaag atgctgaatc
    24901 agactttctt gtttcgtttc ctttaagcag actatgattt gccttaattc gaaaccttca
    24961 tgaaaaagct ttccaatctc tagtgacaaa taagccgatt gatacaagga atatatcggt
    25021 ccaatcaata attgtctgct tatccattca ctctgatcaa caggagacaa cttcatcaga
    25081 cgatacctta ctagtaaagc caatagagta accgctagga tgaatagtag aacataccaa
    25141 tgaaagactt gtatcagctg aataccccaa tgctcttgca tgaccattcc tgaaccaata
    25201 agctgaggca aaacgaacaa ccgcatacta aacaagatac ctagcaaaaa ggtgagcaat
    25261 agcatcggat atgcaagcag ctttttcaaa tcattgcgaa atccttcaac aagccgaaac
    25321 tgtttggcaa tttccaacaa agttgtcggc agattcccat gacgttcagc taactcgatc
    25381 tgtaccagtt gctctgctcc atatcctatt tgcgaaagta cctcactaaa cgcttgccct
    25441 tcctttagct gatgttggat atttgccaaa ataacaggtg gaaacgaacg gatcgtgagt
    25501 aataaataaa gggattcttg cagactaaaa ccagataaaa gcagttctcc catggtttgt
    25561 gcaaactggt tttgctggct tcttgttaat cctttagaat agttctgact gccaaatatt
    25621 ttcctgagct tcttcataac tttcctccca tgattttttt actgattctt cctccataaa
    25681 ttgataactc cataaaacag aaggatggtg attggcatct tccaaaagtt cttgataaat
    25741 gacaccagct aaacattcag atagctcttc ttttttcttt gtcagttcat ggattctgac
    25801 caatgtacct tcaagattgc gtgcatgtac ggttgcaaaa acccggtgcc ctgttagtgc
    25861 tgctcttacc gcagcatgtg cagttgattc atctcttatc tcgccaatga ttagcaagtc
    25921 tggacgatgc cgtaatgcga gtttcaaaag tgtatcataa gtctgtgcta ttttcggatt
    25981 gatctgtagc tgcagaaaaa acggttcttc tatctctaca ggatcttcga tagtaatcac
    26041 ttgcaatttt tcttccaaag ccaaccgata cattagagaa gtcttgcctg atccgactgg
    26101 tccgctgaaa aggtataatc cacgtctgac ggttttctgt aaaatagttg caaaatctcg
    26161 agaactaaag caacgatggg gtgtttcaaa agtaccgtat aatagccgaa tcactaaact
    26221 ctctcgttga aggtaatctc ctacagaaga caaccgcaga cgaacttcct gtttttctaa
    26281 agaataagta aatgcaccta actgagcttt tcttttttct ccgatatcca tctgtcccaa
    26341 atacttaaat cttgagatca gttgctgtcc atctgccgaa gagactttct tccagaatgt
    26401 tcgttttttt ccatagcgaa aatagatgta aaatacttct gaagctggca atatatatac
    26461 gtcttgggca tgtttggcaa aactttcact tatcatttga tccgataatc gttttatttc
    26521 cattattctc ccctccacaa aaaacatacg tctttttgat ctttttgttt caaataaaat
    26581 gaatttacaa aaaaatattg gcaaaagccg aatttatgtt atacttataa aaatttatct
    26641 tgggaggcac tccattggaa acaacattaa ggaaatatat cgaagtagca agtaaaaaaa
    26701 agcctgctga cctagtgatc aagcatgcta agatcgtcaa cgtctttacg aaagaaatca
    26761 tggaaaaaga tgtagcaatc tgcgaaggaa tgatcgtagg aatcggtgaa tacgaaggaa
    26821 aaagcgtaca taatgctaat gggcaatatc tagtgccagg attcatcgat ggccatgtgc
    26881 atatcgaaag ctcactttta tcaccaagcg aattttcaaa agtatccttg cttcatggag
    26941 ttactactgt agtgacagat ccccatgaaa taggaaacat tgctgggagt acaggtcttg
    27001 agtttatgat agaagatgca agacaaacac tcatgaatat cttttttatg ctaccttctt
    27061 gtgtacctgt aacccctttt gagaccaatg gtgcaaattt aaatgcaaaa aaattagccc
    27121 cttttttaca aaaacccgaa gttttaggct tagctgaagt catgaactat caagcagtag
    27181 caaataatga aacagacatc ctagaaaaga tccaattgat gcaccagcat aagaaaaaaa
    27241 ttgacgggca tgcagctggt atcggcatgg aagagttaaa tgtttatcct gcagcaggta
    27301 ttcggacgga tcatgaagct acaactgcaa aagaagctaa agaacgtttg gatcttggta
    27361 tgtatttaat ggttcgagaa gggaccgttg caaaagatct ggcatcgcta ctaccggcga
    27421 tcacacctga aaattcaaga agatgcttct ttgtcaccga tgataaactg atcaatgatt
    27481 tagtaaaaga aggatcaatc gatcatatcg tccgtcaagc tattgcactt ggaatcgatc
    27541 cattacaagc cattcagatg gcaacattga atgcagcaga atgttttgat ttgaaaacac
    27601 taggtgcgat tgcccctggg tatcaagctg atttcttctt aacagacgat cttaccaagc
    27661 tgccaattca tgaagttttc tataagggaa cattagttgt tcaatctggg caactccaag
    27721 accatttatt tgctgatcat tcaaattcat atacgtgttg tctgccaaaa ctaaacgctc
    27781 aaccattaaa tccacattcg ttagagctgc ttctttcttc ttcacaagcc catgtgatcg
    27841 aaattatccc gaatagtcta gtgactaaag atttggtaga aaaagtggac cgacaaggcg
    27901 aacgattcat tccttcaatc gaaaaagacc aattaaagat tgcggtgatc gagcgtcacc
    27961 atcaaacggg aaacattgga ttaggcatag tgaaaggatt tcagctgaaa caaggagcga
    28021 tagcgacaac gattgcccat gactcgcata atctagttgt cgttgggaca aacgacgagg
    28081 atatgctgta tgctgctaat cttttgattc aaaaaggtgg cgggatgacc gttgtcaaca
    28141 aacagcagga aatagcttgt gtttctcttc ctataggcgg aatcatgaca caggaacctt
    28201 tttcgatcgt ctatgaacag ttgaatgaat taataactaa agcacatcaa ctaggagcag
    28261 ctaaaacctt tgatcctttt ttgacacttt ctttcttaac attatctgtc atacccgaat
    28321 tgaagatcac cgacaaagga ttattttcat tttctcgctt ccaattgatt ccaccttgtg
    28381 gaaaaaccga tgaaataaat acattttaaa tcaaataaat cccagaaaat acattacatc
    28441 tgtaatgttc attttctgag atttatttat ggatattaat gtttttatga aagcagaaag
    28501 ttagtttcac agacttttct ttttggtctt atcttgaaat tgagcttcta ctcgataaat
    28561 acgttgacct tttgaagaaa atttttcttc atactccgtc ataatattgc cttcaaattg
    28621 actttgatgt aaatctaacc atacctgttt gaggatcatg ccatattgag aaaaacttgc
    28681 cagagaatac tcaaacaatc cttgattatc tgttttgaaa tggatttctc catttggacg
    28741 aagaatctgt tcatctacag caagaaaagt cttatatgtc aatcgccgtt tttcatgacg
    28801 tttttttggc catggatcgg aaaaattcag atagatttga tctacttcgc tgtcagcgaa
    28861 atactctgtc aacgctgatc catccacatg tagaagtttc aggttaggaa gcggttgttc
    28921 gatcaacttg tctagtgcaa tcgaaacaac actgacctgc atttctactc caatataatt
    28981 gatttcagga tgggcttttg ccattccagt gatgaactgg cctttcccca taccaatctc
    29041 aatgtggatt ggatgatcgt tttcaaacaa ttcattccat tttcccttcc acaatgttgg
    29101 atcactaatt acaaaattcg ggtgagccgc taacatttcc gctgctcccg gacgatttcg
    29161 gacacgcatt atttatctcc attcctttcc tctaattaaa aaggtctggc caaatctccg
    29221 cagagtttgg gccaaacctc agccactgaa aattcgtttt aattgtaaaa tttcttcgtt
    29281 catccgtcga tgatcttcac gtttgcaata tctggtgatt tcttggagca aattcatggc
    29341 cgcataccaa tgaaattttt caagcatttc gtcagtcggg cgtatgccat aactgatcag
    29401 ccactgattc cagctagaca gtggcacata atttcctaaa atcgttgcaa tatccaccgc
    29461 agggtctgca aacatgattg aatcccaatc gactagatat aaataatttt tacatactaa
    29521 ccaattacga tggtttacat ctccgtgtac tactgataag ttttccttat ctattgacgg
    29581 gatctgctca cgaagaaaac gatggacaat ttgcagaaat tgatttttct ggagttcttc
    29641 cggtaattct ttttcatatt tttgcagcat cttctccggc gtcatgacac gaccaccgat
    29701 acgggtcagc atggttttaa gagaatttga atgatgtaaa tggtacaaaa catcaatcac
    29761 gtcgtttctt tgaccaatct cattaggagt cagcaagcga ccttccagcc actcttgtgc
    29821 tgtcaaagta tcgccattcc ccgttcgttt tgtccaaaca agttttggtg taattccttc
    29881 ccttgaaaga gcagccaaca tcggagtcgt gtttcgttta ataaagactt tgtcctcatc
    29941 acgcatgcct atgtatgttt ttcctgtgtc gcctttgatg ggacgtaacc gccactcttg
    30001 gtctaactga aactccatca tgcattctcc cgtcctctta ctctttgttt tgcagtactt
    30061 attattctag tcgcctgaac actacacgtc aagcacatat acgttctttt tcatttaatt
    30121 gccattcaaa aaaccagctt ttctcaaaaa aaatagaacg aacagcattc ttactgtcaa
    30181 cgctctattt ttcattctca tttttaccct acacagcttt gttttcagtc tgacatcttc
    30241 ttcaaacgag ttggtacata tagtttaaca aagagcatag taacaatcaa ataagccgct
    30301 agcggaaccc atcgctgctg cccatctaat acgactgcag caatcaaacc aaaaataacc
    30361 gacgcgataa ttaataccca tgtgagtaga tactgaatcg cctgcttctt ctgattctta
    30421 tggattggat aaagctgaac gagaaccata tagcggaact ggttcgctaa aggtaacagt
    30481 tggaaaccga tcaaatataa aaataagaaa ccgataatca gactaaaata aaagtcgact
    30541 actccggcaa tcaataaccc acctataatt actaatcgga aataaagacc actaaaatca
    30601 gaacctcttg ccaatcttct tgcaaacaag taaagataag tgttttttga ttctctttta
    30661 atatgagtca atataggatc caaatagcgt cttcttttga tctttgccgt aatttcagga
    30721 acatctgtga ataaattgat aaattgatag atctgatgca acctatgttc ttctacatgc
    30781 accattttct cccaatccaa tcctttttct tgttgcttcc acaaaaacca gtaatataga
    30841 cttgcctgta ctaatgcacc tgctattcct gcccatatta gccaataaaa tgatagaaac
    30901 aaaaagatca gagaagaaaa aaaccagcct aatttccaat aacgaataac ttgttgatct
    30961 agtcgaaaca attctgctct cttgatttgc agatgactga atttcaaact ccataatatg
    31021 atcagataga cgaaaaacat cgaaaaggat tgctccgtac taaccaccac taatggcatg
    31081 gccaatccgc atgccaatat caaaaagatc aacggaaaaa tgcaagaata gcgaaaagct
    31141 cgttccaaat aggtttgcat ttctttttct ttaggaagta aaaaaacaat atctgctgcc
    31201 tctgcaaaag tagccatctt acccacgaat aaacagagca tccaaaatac agagattatg
    31261 agccctccaa ttgcaaacgg tggctgcagg gttttcaacc aattagaata atataatccc
    31321 acaccgccaa tcaagaaaag acaaactaaa acaaaatgat cgttcaatac ataacgcata
    31381 tatttcatca ttttcttttg atgtcttgct aaacgttttt gaaaaaaact actcatgtgc
    31441 ctgctccttt gttaaagcaa tatagatttc gtccaaagaa gaatccggca gctgaaattc
    31501 ttttctcaac atctcaagag aaccgtctgc tcgtatctct ccgttatgta agaccacaaa
    31561 acgatcacaa tatttttctg cagtagctaa aatatgcgta gacatcaaaa taccggctcc
    31621 ttcttttttc atttctacca tcaattcaag caatgcattg atagctagtg gatccaagcc
    31681 taaaaatggt tcgtcgatga tatataaaga aggctcgatc aaaaaggcgc ataagatcat
    31741 cactttctgt ttcattccct ttgaaaaatt agctgggaac cagtcaagac gattagataa
    31801 ccgaaaagtc tccaacaact tttctgctct tttcattgct atatccatag gtatatcgta
    31861 ggccatcgca gtgacttcga tatgttctct caatgtcaat tcttcatata aagaaggagt
    31921 ctctggaata tacccaattt gtttgcgata ttcttccgta tttttcttta aggatagatg
    31981 attgatttct atcgttcctt ttgaagcttc cagcagaccg ataatgtgct tgatggtcgt
    32041 acttttccct gctccgttca gtccaatcaa ccctactatc tctccaggct gtacatcgaa
    32101 actgatatct tttaacacag ggagatgtcc gtacccccct gtgacatgat cgataattaa
    32161 actcattctt tttcctccgc catttatttt tatttattat accacgaaga aaatgacggt
    32221 attttgtttt tttccttaca atagctttaa cttatggtaa agttaagctg aagtatttat
    32281 tctttataga aaggatgttt tatatggaaa actgtatttt ttgcaaaatc atcaatcaag
    32341 aaatcccaag ctataaaata tatgaagacg acaaggtcta tgctttttta gacatctctc
    32401 aagcaacgaa aggacacaca ttagttgtgc ctaaacgtca tgtagcagat atttttgaat
    32461 atgatcccga acttgcagga gaggtttttt cgcgtgtgcc taagattgct caagcattgg
    32521 aaaaagcttt tccagaaatg aaaggtctta atatcttgaa taacaaccgt gaactagcct
    32581 atcaatctgt ttttcattca catatccatt tagtccctcg ctacagcaaa gaggatgatt
    32641 tttctattca ctttgtcaat catcaagata gttatggatc ggaagaacta aaagctattc
    32701 aagaaacaat cgtaaagcag gtgtcctgcg atgattaaaa actttttcaa aggactgtta
    32761 tttggagctg ctgctggcac tgtcggaggt ctgttattgg cgcccaggag cgggaatgaa
    32821 acaagaaaga aactgatcga agaattggaa gaagcaacaa ctctgacaaa tgatttaaat
    32881 aacagcctga atcattttaa aagcgcattg atcgaaacaa aagaaacagc tgaatctgtg
    32941 attcctgctt tccaagaagc aatcaaaaaa gatatcgaag attttaaatt ccaagccgaa
    33001 ccacgtatca cacaaatcaa tgaacaagta gaaaaactaa aagctgatct gccagagtat
    33061 cctgaacaaa tataataatg attattttct caacagattt aactatataa gtgagaaaag
    33121 tttgcgtatt tcctaggaat gtgatgaaat tttgaatttt gtttgaaact accacgacaa
    33181 cgtttttaaa ggaaatggga tatggtatag ttaatatata aaaataataa ctaattacag
    33241 gagtgcaaat tttaatgaaa aaaaaatcaa ttattttggc agctactagt gcgttggctg
    33301 ttttaactct agcagcttgt tcaggcgata ctaataaaga tatcgcaacg atgaaaggcg
    33361 gaacgatcac tgtatctgat ttctatgatg aagcaaaact tgaatcatcg aaccaatcct
    33421 tagtacaacg gatgatcatt tataaagtat tcaataacaa atatggcgat aaagtaaccg
    33481 ataagcaagt ggatgcagaa tacgacaaac aagcgaaatc attaggtgat acttttgaat
    33541 cacaattaga agctgctgga tatacgaaag atacttataa agaatatatt cgtaacaatt
    33601 tagcttttga agctggcttg aaagctcacg tagatattac agatgacgat ttgaaaactg
    33661 cttggaaatc tttccatcca gaagtagaag cacaaatcat caaattatca agtgaagatg
    33721 aagcaaaaga tgtgaaaaaa tcagcggatg atggagatga tttctctaaa ttagccaaag
    33781 acaaatcaac tgatacagag acaaaagaag atggcggtaa agttaaattc gactcaacga
    33841 caacaactat tcctgcagaa gtaaaagaag cagctttcaa attgaaagac ggtgagatct
    33901 ccgatgttat tactacaaca aatcctactt cttatgcgac agaatactac gttgtcaaaa
    33961 tggtgaaaaa ccaaaataaa ggcaatgaca tggataaata caaagatcaa ctaaaagata
    34021 tcgctactga aactaaacta agtgacaatg cattcacaac taaagtaatc ggtgaagaat
    34081 taaaagatgc aaatgtaaaa atcaaagatg atgcctttga aaatgtgttg agtgcattca
    34141 caacaacttc atcaagtacg aaagactcaa gtgaaactac agcttcaaca aagagttctg
    34201 acacaaaatc aactgattca acaaaagaat cttcaactga agaaacaact gattcatcaa
    34261 aataaaagaa aacaaaaaaa gcgaagactt atgtctcgct ttttttgttt tgaatgaaaa
    34321 aaataaattt ttgctataaa tcttttggta catagaaact tcggttgtcc ataccgaaaa
    34381 tccgatcggt gtattttcct ggttctgttt gacgaatcga agtcaacatc atctgcatcg
    34441 aagcatcgat attgtcgatt tgatgtaata tttctgcttc cattattctt ggacgtacag
    34501 gtgagccata ttctaataag ccatggtgtg ctaaaaccat atgacgtaat acaagaacat
    34561 cttcgtcagc ttcgctgatt ttcaacgcaa gacaggcttt agttatttct tcgtcaatca
    34621 ataccagatg accgatcaaa tttcctgcaa gcgtatattc tgtcgacata ggaccggaaa
    34681 gttcaatcac ctttcctaaa tcatgcaaaa tgataccagc atacaacaat gctcgattca
    34741 gctcaggata ttcatcggcg attgatttcc ctaagtgaag cattgtcaaa gtatgataag
    34801 ctaatccact ggcaaatgca tgatggtttc tttttgctgc cggaaactca aaaaattgtt
    34861 tttgatattt attaagtaga tagcgaacaa tacgattcca atgcgcattg gtgatctcaa
    34921 acaacaattg attgatttct tcttccattt cttctttctt caaaggggct ctttccatgt
    34981 ataaactagg ctctcctggc tcatctaccc ttgttaagcg catatgaaga atctttactt
    35041 gagggtttcc ttgataaacc tctcttttac cgtttagaaa aacgactgtg ccggctgtaa
    35101 atttattgat ttcctcctcc gaagcatccc agtatttacc atcgatagta cctgaagtat
    35161 cttggaaagt aaaagcaata aatttttttc cgtttttagc gattcttaca tctgcacttt
    35221 tgatcaggac aaattgttca aactgctcat cgattactaa atcacgaatt tttttcatta
    35281 tttgctcctt ctaattgttt tacgatttgg ttcatctcat ggtaatagct taccatttct
    35341 ttatcagaag aaaaacaaat tatttgatac ttttctccaa atttagctaa aagttcagct
    35401 aattgatact ttcgctgatg atcataatga agccagccat catcaatgat gatcgggcat
    35461 atcgaacggt tagcttctaa tgagaggtaa gcaaatctca tcgccataat cagctgatct
    35521 ttcgtacctg tagataactc atagatggga aattctgtat atccgtctgt taaagtcagt
    35581 gtatcatttt tcagtaatat cttctgataa tttccttttg ttaaaatacc taaataatca
    35641 cctgctgctc ttaacaactg gggtaattgt ctttcactca tctctgttga aagatcacct
    35701 aagaatgtcc cggcgatttc aagtgctccc cattcctcca gtaattcctg taattttgct
    35761 ttttgccggc tagcttgttg ataaagttcg tccaatgtgc cgtctctttg caactcttct
    35821 acttgaagtt ggagtcgttg ccgattttca cttgcttctt tttcttcttg ctggagttgg
    35881 gattgctttt ctttcagttg gcgcagtctt acagataatt tctcttttgt cagctcttca
    35941 ggaaatagtg gagtaagcat ttccgataat tcattgtatc ttttcatctg ctgttcggct
    36001 tgtttggctt tttgtaaaaa gacaggtact tccgaaggat aagataagcc tgcttgagtc
    36061 aattgttcgc tgtattcttg tatccatact ttttgtttct ttttcaattg attgatttct
    36121 tgcttcaata aagtattttg ctgatagctt ctggcaaatt tcatctgttc catctcttgt
    36181 ataaaacggg aaagcaatac cattttttct gaaattgtct tatcttgtat cggtaaccat
    36241 tctttaacaa actcaaattt ttggtcgatt tgtcgtagtt tttcttgtat ttcctgttct
    36301 cttttatgtt tccgctcaaa tacgccatat aatttctgat aatctatgac ttcctgtcca
    36361 tattctttca tgagtttgag cgtatccatc tttcctaagt gccgatgatc cgcaaaacga
    36421 tatacttctt gctgaagaat ctgtttttct tctccagttc tcttttgttt ttcttctgct
    36481 tcagctaatt gagctaaggc tagatcaagt tgtcctagtt tttcttgcca cattcctttt
    36541 atctcagaga aatcattttt agttttaatt tttggttgcc ttttgtagaa gaaatagcca
    36601 atcatgcctg caccaattag taaaaggatc actttcaagg gcattggtac aaatatacct
    36661 gcaatgatta ttgctccgcc agccatcagc atatacggtt tagatgaaga gactggtgct
    36721 atctctgact gattttgtaa taatgctgga taggtctctt ccaagtcatt cacttctgct
    36781 tcaatcatct cttgctgttc tttagctatt tggagacgca cagaaatctg gctttctaga
    36841 tcagcaatcc ttctttcttc agccagaaga tgcttccatt cttcttcata taaaaattct
    36901 tccggtaacg gatttttctg ccaatgccac ctttgttcca aaatcttcaa ttctgaattt
    36961 gtttctctag tctcaatttg taatctttgc cattcttcgt ttaattgttc cgctgtaaat
    37021 tgaagttgct ggagatcttt tagttcctgc tcctgctcta aataaaaata atatctggct
    37081 gactgctgat ccatcccgct atgcttttcc agtgttgctt ctaattcttc caaacgctca
    37141 tttgtttgct gataccgttt ggagaaagac tccagatcta aaagaagttg ctcatcagga
    37201 agacctgtag tgctgttttt tttgagagat tgccattctt catataaaga aaaattaagt
    37261 tcttgctgtc tggctaatga aaattgtttg ttcgcctctt ttaattgtgc caataactgc
    37321 tgctgttgcg actcgtattc ctgttcttgt tggatcaact tgccaaaata agcttcctgt
    37381 tcttgttttt gatgaatttt ttcttgcagc tctttccatt catttaattt ttgattgatt
    37441 agtaattttt ggcctttttt cttgaataat tgttgtgctt tctgataata ttcttgccgc
    37501 ttttggaata cttgagaact gcccgtgatg cctaaagaaa tcagcgcatc atgtagctct
    37561 ttttctctca aatgatctag ttccatcaac tgctcttgct ggaaagtaaa tacctgctgg
    37621 aataactctt tcgtcaaagg tgcgatcagt ttttctagta actcatcact tcccacttga
    37681 tcccccagcc acaccttcga ttttccgcga ttttgctgct tgtagcgctc gacagcaaat
    37741 tcaccatata caggatgttt aagccatatt ttgccgccat aggctgagcc gtcttttggt
    37801 gtataatcct tttttttacg gccttttgaa gggaaaccaa ataatattgc ttgaataaat
    37861 tgataaatcg ttgattttcc tgcttcgttt cccccataga tcagctgatt gcctgaataa
    37921 aagtcaaaac ttttttgacg ccattttcca aaagcagcaa tctcgatcct taaaatcttc
    37981 attcctgatc ctcctcaaat aaaaaatctt gattaagcag ctgcttggct ttttcaagtg
    38041 tttcttcttg atattccggc agttcgctca atattctagc cgcttcttga tgactatata
    38101 cttcatttaa aatttgttgg aaatcttgag gtgtctgata gtattgaaat aactgttcca
    38161 tcaatttagc agaagcagca agtggggtct tcgtataaaa agtattctct atgagagaaa
    38221 tacgccaaat aaagaaatca tttatcctat ctgagaattc ctctaacaaa tagtctaata
    38281 gctctcctga ttgtatccgt tccaaaacat ctgtattgag atggtcataa tcttttaatt
    38341 ttatctcaat taaactaaat ccatcctcta cttttgaaag ctgctgacga atgtgaacaa
    38401 tgacctcttt tgtcgttctt gctttcctca atgatatttc ctttgtcttc caatagacct
    38461 ctgctacttt aatcggatgt atttggcaac ttcctttact taattcaact agtagaatag
    38521 atgtagaagc ttgttccttt ttagtatgac cttgaggcgc acctggataa acaattgttt
    38581 gcttttcatt taagaccgtt ggcacatgga tatgccctaa agcccaataa tcgtatccct
    38641 tttcttgcat ctttgagggt tggaaaggag catagtttcc tttttgagag acgccaggtt
    38701 ccccgtggta caacccaata tggtaatctg tgagttctct agaaggaaat tccatcactt
    38761 tgtctttttg aatccacggt tgacgataac taaatccact gagagagaca gtttctccac
    38821 tttttatcgt tatttttttt gtctctaccg tttcggatgt aaataaatgg acatttttag
    38881 gaaaggagaa ccaataccgc tctttttgat agaaatcatg atttccgaat attataaata
    38941 caggtatatg gttcttttct aaccgtttca tctgttctga aaaatgtttc tgtattttta
    39001 atgaaggacg attttgatga aaattatctc ctgcaagtaa aacaaaatca acgttattta
    39061 ttattgcctg ttcaatgata tttgataaaa cggtcagatt cgtcttcaat agtttttcct
    39121 gtacagtttt atcaagagtt gtcagtcctt caaatgaccg atccatatgt aaatctgccg
    39181 catggataaa tttcaccatt aaaaatttcc tccttttttt cttttatcat accattttca
    39241 atgttttttt tcatcaataa ccgaaaattt gttcgtatta tgcaaagaaa ttacgataaa
    39301 tggccgactt caaaaaataa gtttattcga aaaaaagatt gtgacatgat ttttgtcaca
    39361 atcctaaata tctatgttat attcgcttcg tttgacactg aagaagtact atttgtactg
    39421 gaatttatgc tccatttttc cgtatttaga attctatgtt ctatactacc ttaattagtt
    39481 tatatggtaa gaattctttc aaatgcttga gatactccgt ctttatcatt tgaaacagtg
    39541 acataatcag cttttttcat gatttcttga tgactatttc ccattgcaaa actaatatct
    39601 gcactttcaa acatagataa gtcatttaaa ctatctccga tcgcgattct caaaggtttc
    39661 tttaaattgt agattgattc caaacgatca aacaccacac ctttagatac attttgaggt
    39721 acgatttcaa tatttacagg tgccgattca gtaacagaca catttagcat ctttttcaaa
    39781 tggttaaata tattagcttt caattgacga ttaggaataa aaataaaaaa tttgtaaatg
    39841 ctgatagcat ccttttctat aatttcctca atattattat aagggattga acaaatagat
    39901 ttttcatact caagctcata gtttagctct ttgatagaaa attcactccc tacctcgtga
    39961 aaaatttttt tcattttatt tttataattc tgagattcat aacttccgag atttgtatat
    40021 attttggttg gaaatttcga taggacagaa tcaatttttt ttacatcatt tttatcgaag
    40081 gaagactcaa atatcttttt ttctccatca aatccaacag aaccatttaa ccctactgct
    40141 ggtatcctta aaccagattt tttgagaata ttattaatgt cttctacttc tcttccagaa
    40201 caaatgaatg gaaaacagtc ttccggtaaa tgtttcattg ttcttgcatt tttttgacta
    40261 atgctctggt cagaattcaa aagagtaccg tctaaatcta taccaattag tttcatttca
    40321 tccatgtctc ctatacgttg attataaatt cactgcttat tgtaagtcta aatttctcac
    40381 aaaacatata ctaattatac ctaatttaag taagtacaaa catcatttta agaataggaa
    40441 aggacattta tatactattc tctgtatttt tgatttataa ataaatatga attgaatcca
    40501 aatcagctat attactattt tataataacc aactataaaa aacgaagtta ggtgaaaaac
    40561 taacttcgat attcataaaa gactctatta gttcaactta gcaaaagatt taaacaaaaa
    40621 aagatgaact atttctagtt catc
//
LOCUS       DACEDO010000018        37294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEDO010000018 DACEDO010000000
VERSION     DACEDO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.018"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..779)
                     /locus_tag="IWO52_001033"
     CDS             complement(45..779)
                     /locus_tag="IWO52_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0355314.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IWO52_001034"
     CDS             1106..1516
                     /locus_tag="IWO52_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0355315.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IWO52_001035"
     CDS             1509..2210
                     /locus_tag="IWO52_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0355316.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IWO52_001036"
     CDS             2210..3796
                     /locus_tag="IWO52_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0355317.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IWO52_001037"
     CDS             3815..4036
                     /locus_tag="IWO52_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0355318.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IWO52_001038"
     CDS             4033..4794
                     /locus_tag="IWO52_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0355319.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IWO52_001039"
     CDS             5174..5683
                     /locus_tag="IWO52_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0355320.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IWO52_001040"
     CDS             complement(5758..6297)
                     /locus_tag="IWO52_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0355321.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IWO52_001041"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IWO52_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0355322.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7364..8161
                     /locus_tag="IWO52_001042"
     CDS             7364..8161
                     /locus_tag="IWO52_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0355323.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8189..8824
                     /locus_tag="IWO52_001043"
     CDS             8189..8824
                     /locus_tag="IWO52_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0355324.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="IWO52_001044"
     CDS             8844..9617
                     /locus_tag="IWO52_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0355325.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="IWO52_001045"
     CDS             10025..10822
                     /locus_tag="IWO52_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0355326.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="IWO52_001046"
     CDS             10800..11213
                     /locus_tag="IWO52_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355327.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="IWO52_001047"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="IWO52_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0355328.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="IWO52_001048"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="IWO52_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0355329.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="IWO52_001049"
     CDS             15990..16550
                     /locus_tag="IWO52_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0355330.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IWO52_001050"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IWO52_001050"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0355331.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="IWO52_001051"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="IWO52_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0355332.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="IWO52_001052"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="IWO52_001052"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0355333.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="IWO52_001053"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="IWO52_001053"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0355334.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="IWO52_001054"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="IWO52_001054"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0355335.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="IWO52_001055"
     CDS             21897..22475
                     /locus_tag="IWO52_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0355336.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="IWO52_001056"
     CDS             22527..23009
                     /locus_tag="IWO52_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0355337.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="IWO52_001057"
     CDS             23126..23500
                     /locus_tag="IWO52_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355338.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24178..25530)
                     /gene="brnQ"
                     /locus_tag="IWO52_001058"
     CDS             complement(24178..25530)
                     /gene="brnQ"
                     /locus_tag="IWO52_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0355339.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25677..26267
                     /locus_tag="IWO52_001059"
     CDS             25677..26267
                     /locus_tag="IWO52_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0355340.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26395..27744)
                     /locus_tag="IWO52_001060"
     CDS             complement(26395..27744)
                     /locus_tag="IWO52_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0355341.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27912..28652)
                     /locus_tag="IWO52_001061"
     CDS             complement(27912..28652)
                     /locus_tag="IWO52_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355342.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28665..30257)
                     /locus_tag="IWO52_001062"
     CDS             complement(28665..30257)
                     /locus_tag="IWO52_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0355343.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30825..31121)
                     /locus_tag="IWO52_001063"
     CDS             complement(30825..31121)
                     /locus_tag="IWO52_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355344.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31297..32022
                     /locus_tag="IWO52_001064"
     CDS             31297..32022
                     /locus_tag="IWO52_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0355345.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32015..32857
                     /locus_tag="IWO52_001065"
     CDS             32015..32857
                     /locus_tag="IWO52_001065"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0355346.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32860..33381
                     /locus_tag="IWO52_001066"
     CDS             32860..33381
                     /locus_tag="IWO52_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0355347.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33634..34197)
                     /locus_tag="IWO52_001067"
     CDS             complement(33634..34197)
                     /locus_tag="IWO52_001067"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0355348.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34448..34717
                     /gene="rpsO"
                     /locus_tag="IWO52_001068"
     CDS             34448..34717
                     /gene="rpsO"
                     /locus_tag="IWO52_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0355349.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34905..37031
                     /gene="pnp"
                     /locus_tag="IWO52_001069"
     CDS             34905..37031
                     /gene="pnp"
                     /locus_tag="IWO52_001069"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0355350.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
BASE COUNT        12150 a         6942 c         7772 g        10430 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt gggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagttt
    23821 ttttgcgaaa actattttta tatcatatat atgtctaaaa tctatattat aaaagaaatg
    23881 ttgtttgatc agcttctctt cggtattagt tcaaaacaaa acggcaaagt atatggagct
    23941 actttgccgt tttgtttctc taatactcag gtcagtttat agacgctcaa taaacggtgt
    24001 tcaaaagcca gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc
    24061 aaaaaatatt tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt
    24121 gttcaagaga cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca
    24181 atctgctact tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac
    24241 tgctggtacg atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata
    24301 taaaagattt tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc
    24361 aaaaatcgaa gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt
    24421 aaacaaagga ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat
    24481 caaaacaggc aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg
    24541 aaggatacta gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa
    24601 tgtttctgca aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat
    24661 aaatgctaat aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt
    24721 gatcccgcca ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag
    24781 caaagtataa atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt
    24841 agcgatttca gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc
    24901 aaatgctaaa gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa
    24961 agcatgctct tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa
    25021 aaaagcaagg agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc
    25081 aacataatct aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac
    25141 aatactgaaa attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc
    25201 aatctcgaaa gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta
    25261 aagcaagatg gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac
    25321 acctgagctt ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt
    25381 aaccaagaaa cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac
    25441 gggaaagata agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag
    25501 atagtcttta aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt
    25561 aacaaaattt cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt
    25621 atttaataac gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga
    25681 caaagtcgag attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg
    25741 tattagagat caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata
    25801 cattattagc ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc
    25861 gtatatttca gatggcagaa cgaattaatt accgagtggt ctggtcgaat atctttttgc
    25921 tcttttggct gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt
    25981 tttggcctga gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct
    26041 tgatgaggca aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa
    26101 aggaccgtcg aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt
    26161 ttgtaccgtt gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct
    26221 ataaaaaagc agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg
    26281 acagaagctt ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    26341 tcgtaaattc cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt
    26401 cttacgttca tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag
    26461 taataaagcg attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta
    26521 aagaaatacc agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt
    26581 gccgaggata cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa
    26641 gaaacaagta gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt
    26701 caagataaca gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg
    26761 atgtgttgtt aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag
    26821 catacgtcct gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac
    26881 taaattgatc aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg
    26941 actgttatct ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc
    27001 taagtaaaat aagatgatcc tgataggaat attattgata gcttccggca acactttttc
    27061 aggattttct gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt
    27121 taccatttgg aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga
    27181 ccatagattc gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata
    27241 agctcctacg aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc
    27301 taactcacca aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat
    27361 cagttcagga agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc
    27421 tgttaaatcg gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa
    27481 agccgctttt ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt
    27541 tgataacaga agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag
    27601 ataggcgagc aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa
    27661 ccccgttcca atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg
    27721 ctgcagttct tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt
    27781 ccatattaaa agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac
    27841 cttttatgag taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc
    27901 agctattgat cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc
    27961 ggatggacaa agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa
    28021 atgacacggt cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc
    28081 aagccagtat gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct
    28141 aacgccgaag taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca
    28201 attgcgacac gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga
    28261 tccataccaa ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt
    28321 ttcaggacag ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg
    28381 ttgaaggatt ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga
    28441 gctaatacat tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg
    28501 atacagcgca agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca
    28561 cctttattga ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt
    28621 agatgtttga tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt
    28681 gaattgtcga tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc
    28741 gcccgcagga ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta
    28801 aatgtttgga agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca
    28861 gctacgccga ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct
    28921 gttgctggca agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca
    28981 tgggtcatac caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc
    29041 ccacgaacga tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca
    29101 atcgttcggt tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct
    29161 tgtaccatca tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg
    29221 attttttgga agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca
    29281 atcagtaatc caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt
    29341 ccagcacctc gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg
    29401 atgccaggtt tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata
    29461 attttggttc gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc
    29521 gggtcgcctt tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca
    29581 tctgcaccgc tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct
    29641 tccggacgtt cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct
    29701 gagaaattat ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag
    29761 tggaatgtat ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct
    29821 tttgcatatt tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg
    29881 gtaaaatcaa tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg
    29941 atcttgcctg attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat
    30001 tttttcccta aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa
    30061 ctattttggc cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag
    30121 ggtgcatacc cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca
    30181 tttgccagag atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa
    30241 accatatttt ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga
    30301 tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa
    30361 ggccgctaaa attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca
    30421 ctgctaagac agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag
    30481 tgcacatttc ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt
    30541 taatgattct cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac
    30601 aaaaaaagtt tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa
    30661 atactgataa atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc
    30721 tatccattgc aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag
    30781 aactagaaca gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc
    30841 tttggctcac gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt
    30901 agataaatca aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat
    30961 gtaatggcta aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca
    31021 ttttctttgc gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata
    31081 caagccagaa taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt
    31141 atacagcagt attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt
    31201 caaatgccca taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc
    31261 gagtatgggc tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa
    31321 gcagcaaaag agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc
    31381 acaccttctg gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag
    31441 ccggaatact tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt
    31501 gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat
    31561 ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag
    31621 gctaaaagcc cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa
    31681 tgtcaacaaa cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga
    31741 ggacaaatac gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag
    31801 caggcggctt atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa
    31861 gttcatccgt tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc
    31921 aatcaaacgc ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt
    31981 gttttcaaaa aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga
    32041 aaaaatgatt gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat
    32101 gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca
    32161 attgttgaaa gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa
    32221 ttttgattac ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac
    32281 atttttagat gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca
    32341 gctttacacg gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc
    32401 aaaacccatt aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg
    32461 tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc
    32521 cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc aagacagtag aggaagaact
    32581 aacagaagag tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttgat
    32641 tgctttatta gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga
    32701 aaaccaagca gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct
    32761 gctgaaaaat aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca
    32821 ctcgcgcgaa taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg
    32881 cgaaaacaaa tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt
    32941 gcgaaaactt ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct
    33001 gcaggtaaaa gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat
    33061 actgggcaca tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg
    33121 agactaatcg ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac
    33181 ggtgagttag caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga
    33241 cgttatcaaa aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat
    33301 acgatccgct acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca
    33361 ggaatcctct acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat
    33421 catctctgag ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc
    33481 caatacacag aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg
    33541 gagaaacata aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct
    33601 tgtagcatag tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt
    33661 tagtgcaaat ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc
    33721 gatttcatgt tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc
    33781 attcatatca taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc
    33841 tcgatcaaca gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct
    33901 caaaatttta ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg
    33961 acttggtacg tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc
    34021 aacaccgcct cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa
    34081 ctcaagcatc tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt
    34141 tgcaacttcg cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga
    34201 atatgtaccc ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca
    34261 caaaaatgga aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta
    34321 cttgattttc aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc
    34381 cacttggtta agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt
    34441 gaaagaaatg gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca
    34501 tgaaggggat actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca
    34561 cttgaatgaa catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa
    34621 aaaagttggt caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta
    34681 ccgcgaattg atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt
    34741 cacatgaatc tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta
    34801 gatggactcc tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt
    34861 tgtttagtag cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag
    34921 tattcaaaac gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac
    34981 aagcaaacgg agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg
    35041 cctctaaaga agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa
    35101 tgtatgcagc agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg
    35161 aagcaacatt gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct
    35221 tccgtaatga agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag
    35281 ctatggcagc aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg
    35341 gcccaatcgc aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag
    35401 tagaacaagc agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca
    35461 atatggttga aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt
    35521 ttggttttga cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag
    35581 ggaaagaaaa aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct
    35641 ttgatgcttc ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg
    35701 aagataatat cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag
    35761 gtcatgaaga tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa
    35821 aagatgtcgt acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg
    35881 atgaaatccg tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt
    35941 tgttcacacg aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc
    36001 accaaatcat tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt
    36061 tccctcaatt ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg
    36121 gacatggcgc attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc
    36181 cttatacgat ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa
    36241 gtatctgtgc tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg
    36301 ccggtattgc gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc
    36361 aagggctgga agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta
    36421 tcactgcttt acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag
    36481 cattaactca agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg
    36541 ctgctccacg tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac
    36601 ctgctaaaat caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg
    36661 aaactggtgt gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg
    36721 cagaaatgat caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg
    36781 gtgaagttta tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga
    36841 tcaaaggaaa agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag
    36901 tcgaagatgt tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac
    36961 aaggacgcgt aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg
    37021 aagaaaaata atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga
    37081 caataattat tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg
    37141 aataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt
    37201 aggagctgtt tttatttggc tgttcttatt acttgatgca gaacagcttt tttacaacct
    37261 ctccaataaa cgatgttcaa tcagctgtcc ctcg
//
LOCUS       DACEDO010000019        37289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEDO010000019 DACEDO010000000
VERSION     DACEDO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.019"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            107..970
                     /locus_tag="IWO52_001070"
     CDS             107..970
                     /locus_tag="IWO52_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0355351.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IWO52_001071"
     CDS             complement(1099..2721)
                     /locus_tag="IWO52_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0355352.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IWO52_001072"
     CDS             2970..3491
                     /locus_tag="IWO52_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355353.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IWO52_001073"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IWO52_001073"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0355354.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IWO52_001074"
     CDS             5572..6390
                     /locus_tag="IWO52_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0355355.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IWO52_001075"
     CDS             complement(6493..6690)
                     /locus_tag="IWO52_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355356.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IWO52_001076"
     CDS             6859..7437
                     /locus_tag="IWO52_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0355357.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="IWO52_001077"
     CDS             complement(8482..8985)
                     /locus_tag="IWO52_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0355358.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="IWO52_001078"
     CDS             9344..10369
                     /locus_tag="IWO52_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0355359.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNLMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10692..11570
                     /locus_tag="IWO52_001079"
     CDS             10692..11570
                     /locus_tag="IWO52_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355360.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12046..12162
                     /locus_tag="IWO52_001080"
     CDS             12046..12162
                     /locus_tag="IWO52_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0355361.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13090..13428
                     /locus_tag="IWO52_001081"
     CDS             13090..13428
                     /locus_tag="IWO52_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355362.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13522..13752
                     /locus_tag="IWO52_001082"
     CDS             13522..13752
                     /locus_tag="IWO52_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355363.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14152..14523
                     /locus_tag="IWO52_001083"
     CDS             14152..14523
                     /locus_tag="IWO52_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0355364.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14797..15489)
                     /locus_tag="IWO52_001084"
     CDS             complement(14797..15489)
                     /locus_tag="IWO52_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0355365.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15492..16277)
                     /locus_tag="IWO52_001085"
     CDS             complement(15492..16277)
                     /locus_tag="IWO52_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0355366.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16514..17914
                     /gene="lacG"
                     /locus_tag="IWO52_001086"
     CDS             16514..17914
                     /gene="lacG"
                     /locus_tag="IWO52_001086"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0355367.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17939..18256
                     /locus_tag="IWO52_001087"
     CDS             17939..18256
                     /locus_tag="IWO52_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0355368.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18269..19942
                     /locus_tag="IWO52_001088"
     CDS             18269..19942
                     /locus_tag="IWO52_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0355369.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19956..21587
                     /locus_tag="IWO52_001089"
     CDS             19956..21587
                     /locus_tag="IWO52_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0355370.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22078..23643)
                     /gene="guaA"
                     /locus_tag="IWO52_001090"
     CDS             complement(22078..23643)
                     /gene="guaA"
                     /locus_tag="IWO52_001090"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0355371.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23702..24625)
                     /locus_tag="IWO52_001091"
     CDS             complement(23702..24625)
                     /locus_tag="IWO52_001091"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0355372.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24821..25426
                     /locus_tag="IWO52_001092"
     CDS             24821..25426
                     /locus_tag="IWO52_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0355373.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25734..26750
                     /locus_tag="IWO52_001093"
     CDS             25734..26750
                     /locus_tag="IWO52_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0355374.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26891..28192
                     /locus_tag="IWO52_001094"
     CDS             26891..28192
                     /locus_tag="IWO52_001094"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0355375.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28238..28900
                     /gene="deoC"
                     /locus_tag="IWO52_001095"
     CDS             28238..28900
                     /gene="deoC"
                     /locus_tag="IWO52_001095"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0355376.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28921..29319
                     /locus_tag="IWO52_001096"
     CDS             28921..29319
                     /locus_tag="IWO52_001096"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0355377.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29385..30467
                     /locus_tag="IWO52_001097"
     CDS             29385..30467
                     /locus_tag="IWO52_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0355378.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30679..32241
                     /locus_tag="IWO52_001098"
     CDS             30679..32241
                     /locus_tag="IWO52_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355379.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32234..33367
                     /locus_tag="IWO52_001099"
     CDS             32234..33367
                     /locus_tag="IWO52_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355380.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33367..34323
                     /locus_tag="IWO52_001100"
     CDS             33367..34323
                     /locus_tag="IWO52_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355381.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34422..35588
                     /gene="deoB"
                     /locus_tag="IWO52_001101"
     CDS             34422..35588
                     /gene="deoB"
                     /locus_tag="IWO52_001101"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0355382.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35612..36430
                     /locus_tag="IWO52_001102"
     CDS             35612..36430
                     /locus_tag="IWO52_001102"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0355383.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36447..37154
                     /gene="deoD"
                     /locus_tag="IWO52_001103"
     CDS             36447..37154
                     /gene="deoD"
                     /locus_tag="IWO52_001103"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0355384.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11629 a         6507 c         7891 g        11262 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacact
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatct
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10501 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
    10561 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
    10621 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
    10681 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
    10741 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
    10801 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
    10861 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
    10921 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
    10981 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
    11041 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
    11101 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
    11161 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
    11221 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
    11281 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
    11341 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
    11401 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
    11461 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
    11521 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
    11581 tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa
    11641 acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
    11701 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
    11761 agcctctttt ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa
    11821 caatagatat taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat
    11881 gaaaatttat ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt
    11941 tgactttcat tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt
    12001 gttttttagc atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat
    12061 atctcaagtg cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg
    12121 aagatcatca aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt
    12181 aaaaaaatag ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt
    12241 tccgaaagaa ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc
    12301 ttttcaaatt gataccattc cctaattcca gttatatagt taactacaga accacaaagc
    12361 acgtacgttt ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt
    12421 ttgaccagta gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt
    12481 tagtcaagaa tggaaacgta actacataat caaaggtttt gcgaaatatt tcatgaaata
    12541 tttcatgtag aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac
    12601 gaaagctccc aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag
    12661 tgattcgtat ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa
    12721 tatgcaaaaa aataaaaaag cagttactat tgctagaata acatcaagga ataataagtt
    12781 ttggttttca gataatagtc gataagttgt gtattggaat tttagataca gtaataaagt
    12841 atttttcatg aaattttgtg agaatcacta ttacaagtat ctttaataaa attaatggtt
    12901 aaggaggaac ttaaagtaga atgtaaatga atagttgtag tcagtcgatt gtgatactag
    12961 actcaaagtt gcaggaataa gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga
    13021 agggcttttt tttatacttt gttgattgta gtgtttattt tccctctgtt tagccaagta
    13081 acattttcta tgactcaacc agtttcaagt gaggaacatg ataacctttt tcgtgctaca
    13141 gaagagttta agaaatatca ggaaaatatt gttccggaga ctccattagc tagtgagcca
    13201 attactgata ttgatggaaa ccaaatagga tggtatatac agtacgaagt tatggtggta
    13261 aataatgact caatagtaaa aaatacagta ggcttagagc taacaccgat tgaaaaggtg
    13321 agttcattgt taacgtatat ttatactgat gaaagtgacg agactgagat cattttaacg
    13381 gattactcaa aaattgctac tgataaaaag tatatattgt ggatgtagac actagtgaaa
    13441 tactttatga tatagaaatt gatgaaaatt tactagatgt agcacaagaa atcgatcatc
    13501 aaaagcagga agctattcga tatgctggta gaaaagggaa aagaaaatat ttattacgtg
    13561 aatgtagcaa aagttagaga agatgaaaat gagtggaaag aattcaagag tcgctactca
    13621 attaactcga ctccgacatt tacagtttac agagaaggat cgatagaaaa aactgttttt
    13681 tggactaaag aaagtggaat gtctttagct gaagtagaag agtttttgga ttatgttagt
    13741 atgcaacagt aagggagttc ttattatgaa aaattttgac acatattatg ttattgtttt
    13801 ttcgatatct ttgctgttaa tattactaac tttaatgcag tataaagagt atccagagta
    13861 ttttatttta acttttgtat atttagtaag tagctacaca cttctatata aattcagaag
    13921 ttcttctaga gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg
    13981 atttatatag gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta
    14041 atagatttaa taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac
    14101 cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat aatgagagat
    14161 tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct
    14221 gataaggcga aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat
    14281 atgagtggtg aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt
    14341 atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa
    14401 ttaattaata tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg
    14461 ttgtttacag ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt
    14521 taaccattac gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa
    14581 gattggtttg agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga
    14641 acgcgtcata tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg
    14701 atatagcagt gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca
    14761 tttcagttgc ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga
    14821 caggtgctct gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc
    14881 ctgttcaaat actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc
    14941 cgcacgtttg gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg
    15001 agctaagctc agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa
    15061 agagccgtcg tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc
    15121 aaaataaaaa gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg
    15181 gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt
    15241 ttgcaagcaa agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga
    15301 ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat
    15361 attttttaaa atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag
    15421 ccaagcagct tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat
    15481 ccctttcata gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg
    15541 acatattgtg catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga
    15601 tcgaaatcat gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca
    15661 acgagtagat atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg
    15721 gtactgtaag tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta
    15781 aactttagtc ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc
    15841 cggaaatcac cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa
    15901 actggataat tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt
    15961 tcgattgttg tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct
    16021 gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt
    16081 tcttgatcta aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc
    16141 gatttttcta attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg
    16201 atgatttcat taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat
    16261 ctttcttttt tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga
    16321 caacagtcaa tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca
    16381 atagatttta ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa
    16441 tgttttggat gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg
    16501 aggagagatg tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc
    16561 ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt
    16621 tttagaggaa caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc
    16681 aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg
    16741 gagccgcatt tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca
    16801 tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt
    16861 tgatacccct aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc
    16921 atttgtttcc tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac
    16981 ctttaatgaa atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg
    17041 aatcaaatat gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc
    17101 ccgtgtagtg aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc
    17161 attagagacg aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga
    17221 cgatgcattg tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga
    17281 aaccttgact gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga
    17341 agaagatttc gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca
    17401 ctaccaatgc cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac
    17461 gggtgaaaaa gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga
    17521 aggaattcct cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt
    17581 gctgcgaatt aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat
    17641 ggggtataaa gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt
    17701 gagagtctat ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta
    17761 ttttttgtgg tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg
    17821 cttgttttat gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta
    17881 caagttggtc agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat
    17941 gaataaagag gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg
    18001 cagtacatta ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc
    18061 ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt
    18121 agcggaagaa gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga
    18181 tcatttaatg accactttat tgctgagaga tctgattcaa gactttattg tactgtacag
    18241 acagaatcaa ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg
    18301 gaaaccattt tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt
    18361 tttaacagcg atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc
    18421 tgagattttt ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa
    18481 ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga
    18541 ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc
    18601 ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga
    18661 atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt
    18721 atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac
    18781 gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt
    18841 aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt
    18901 gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc
    18961 gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc
    19021 ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc
    19081 ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc
    19141 aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt
    19201 tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc
    19261 acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt
    19321 ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc
    19381 ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt
    19441 tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc
    19501 atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga
    19561 aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga
    19621 tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgcgatgtt
    19681 agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg
    19741 tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca
    19801 agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt
    19861 tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt
    19921 tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta
    19981 aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag
    20041 catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat
    20101 ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata
    20161 atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa
    20221 gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa
    20281 atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga
    20341 aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt
    20401 acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg
    20461 ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag
    20521 aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc
    20581 gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag
    20641 gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg
    20701 gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg
    20761 cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa
    20821 cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat
    20881 atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg
    20941 cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa
    21001 atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa
    21061 acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg
    21121 cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg
    21181 gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta
    21241 cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc
    21301 aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat
    21361 tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt
    21421 atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa
    21481 tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag
    21541 gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt
    21601 tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa
    21661 cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac
    21721 gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc
    21781 gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa
    21841 agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag
    21901 ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta
    21961 acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt
    22021 ttagctattg aagaatgttg atttataggg attccccctc catattatgt tggctcttta
    22081 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
    22141 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
    22201 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
    22261 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
    22321 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
    22381 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
    22441 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
    22501 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
    22561 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
    22621 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
    22681 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
    22741 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat tgactttgat
    22801 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
    22861 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
    22921 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
    22981 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
    23041 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
    23101 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
    23161 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
    23221 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
    23281 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
    23341 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
    23401 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
    23461 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
    23521 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
    23581 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
    23641 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
    23701 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
    23761 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
    23821 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
    23881 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
    23941 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
    24001 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
    24061 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
    24121 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
    24181 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
    24241 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
    24301 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
    24361 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
    24421 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
    24481 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
    24541 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
    24601 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
    24661 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
    24721 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
    24781 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
    24841 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
    24901 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
    24961 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
    25021 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
    25081 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
    25141 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
    25201 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
    25261 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
    25321 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
    25381 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
    25441 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
    25501 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
    25561 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa
    25621 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
    25681 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
    25741 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
    25801 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
    25861 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
    25921 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
    25981 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
    26041 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
    26101 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
    26161 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
    26221 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
    26281 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
    26341 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
    26401 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
    26461 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
    26521 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
    26581 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
    26641 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
    26701 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
    26761 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
    26821 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
    26881 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
    26941 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
    27001 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
    27061 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
    27121 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
    27181 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
    27241 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
    27301 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
    27361 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
    27421 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
    27481 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
    27541 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
    27601 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
    27661 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
    27721 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
    27781 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
    27841 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
    27901 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
    27961 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
    28021 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
    28081 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
    28141 tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa
    28201 agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga
    28261 ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc
    28321 aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga
    28381 acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa
    28441 cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt
    28501 cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga
    28561 tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac
    28621 atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc
    28681 tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg
    28741 tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa
    28801 cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg
    28861 agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc
    28921 atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat
    28981 gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat
    29041 caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct
    29101 tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac
    29161 acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc
    29221 cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt
    29281 gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct
    29341 gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat
    29401 tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca
    29461 acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga
    29521 tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag
    29581 aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat
    29641 cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa
    29701 cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata
    29761 caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg
    29821 caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg
    29881 aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg
    29941 ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat
    30001 tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta
    30061 tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat
    30121 tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg
    30181 accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat
    30241 tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg
    30301 acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc
    30361 taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa
    30421 tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata
    30481 gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt
    30541 atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt
    30601 caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg
    30661 ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc
    30721 aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa
    30781 attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc
    30841 ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca
    30901 agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt
    30961 gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg
    31021 cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca
    31081 gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt
    31141 ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg
    31201 acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag
    31261 tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca
    31321 gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa
    31381 ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag
    31441 cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa
    31501 gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc
    31561 gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc
    31621 ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa
    31681 catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca
    31741 ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg
    31801 ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga
    31861 acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt
    31921 atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga
    31981 ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac
    32041 aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt
    32101 gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat
    32161 gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag
    32221 ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact
    32281 ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc
    32341 catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga
    32401 aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc
    32461 agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt
    32521 ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat
    32581 cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt
    32641 tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac
    32701 atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat
    32761 gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg
    32821 cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa
    32881 gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta
    32941 tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc
    33001 agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac
    33061 ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat
    33121 cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca
    33181 gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt
    33241 tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca
    33301 agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga
    33361 actataatgt cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca
    33421 acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac
    33481 gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg
    33541 atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta
    33601 ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt
    33661 atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca
    33721 tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca
    33781 ggattagcag atattccggt cattggacca attttcttta aaaatacatt cttacctgct
    33841 tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc
    33901 ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta
    33961 tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct
    34021 gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc
    34081 atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca
    34141 ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct
    34201 tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt
    34261 gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa
    34321 taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt
    34381 gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc
    34441 tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg
    34501 ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag
    34561 agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc
    34621 aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact
    34681 gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg
    34741 atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt
    34801 atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa
    34861 ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt
    34921 tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata
    34981 tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa
    35041 gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag
    35101 aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa
    35161 tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta
    35221 tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg
    35281 gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc
    35341 cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa
    35401 acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca
    35461 gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa
    35521 cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat
    35581 taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga
    35641 aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg
    35701 ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga
    35761 gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc
    35821 tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc
    35881 catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat
    35941 tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat
    36001 taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat
    36061 gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa
    36121 agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg
    36181 accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt
    36241 agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg
    36301 aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt
    36361 tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac
    36421 attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga
    36481 tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt
    36541 ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat
    36601 ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct
    36661 atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg
    36721 gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca
    36781 gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca
    36841 atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa
    36901 atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg
    36961 aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat
    37021 accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa
    37081 caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag
    37141 cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca
    37201 agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt
    37261 ttgaacaccg tttaccaaga ggttgtgaa
//
LOCUS       DACEDO010000020        37188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEDO010000020 DACEDO010000000
VERSION     DACEDO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.020"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(109..552)
                     /locus_tag="IWO52_001104"
     CDS             complement(109..552)
                     /locus_tag="IWO52_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0355385.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(568..1728)
                     /locus_tag="IWO52_001105"
     CDS             complement(568..1728)
                     /locus_tag="IWO52_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0355386.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2235..3935
                     /locus_tag="IWO52_001106"
     CDS             2235..3935
                     /locus_tag="IWO52_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355387.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(4037..4870)
                     /locus_tag="IWO52_001107"
     CDS             complement(4037..4870)
                     /locus_tag="IWO52_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0355388.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5174..7339)
                     /locus_tag="IWO52_001108"
     CDS             complement(5174..7339)
                     /locus_tag="IWO52_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0355389.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7599..9893
                     /locus_tag="IWO52_001109"
     CDS             7599..9893
                     /locus_tag="IWO52_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0355390.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9886..10551
                     /gene="pgmB"
                     /locus_tag="IWO52_001110"
     CDS             9886..10551
                     /gene="pgmB"
                     /locus_tag="IWO52_001110"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0355391.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10544..11551
                     /locus_tag="IWO52_001111"
     CDS             10544..11551
                     /locus_tag="IWO52_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0355392.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11573..12592
                     /locus_tag="IWO52_001112"
     CDS             11573..12592
                     /locus_tag="IWO52_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0355393.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(12873..14240)
                     /gene="nhaC"
                     /locus_tag="IWO52_001113"
     CDS             complement(12873..14240)
                     /gene="nhaC"
                     /locus_tag="IWO52_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0355394.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(14349..15770)
                     /locus_tag="IWO52_001114"
     CDS             complement(14349..15770)
                     /locus_tag="IWO52_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0355395.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(16013..17890)
                     /gene="tdc"
                     /locus_tag="IWO52_001115"
     CDS             complement(16013..17890)
                     /gene="tdc"
                     /locus_tag="IWO52_001115"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0355396.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(18181..19437)
                     /locus_tag="IWO52_001116"
     CDS             complement(18181..19437)
                     /locus_tag="IWO52_001116"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0355397.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVGALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            20017..21318
                     /locus_tag="IWO52_001117"
     CDS             20017..21318
                     /locus_tag="IWO52_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0355398.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(21387..21986)
                     /locus_tag="IWO52_001118"
     CDS             complement(21387..21986)
                     /locus_tag="IWO52_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0355399.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            22240..23121
                     /locus_tag="IWO52_001119"
     CDS             22240..23121
                     /locus_tag="IWO52_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355400.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            23134..24744
                     /locus_tag="IWO52_001120"
     CDS             23134..24744
                     /locus_tag="IWO52_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0355401.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(24821..25480)
                     /locus_tag="IWO52_001121"
     CDS             complement(24821..25480)
                     /locus_tag="IWO52_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0355402.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(25763..26428)
                     /locus_tag="IWO52_001122"
     CDS             complement(25763..26428)
                     /locus_tag="IWO52_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355403.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(26575..27180)
                     /gene="dhaL"
                     /locus_tag="IWO52_001123"
     CDS             complement(26575..27180)
                     /gene="dhaL"
                     /locus_tag="IWO52_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0355404.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(27208..28191)
                     /gene="dhaK"
                     /locus_tag="IWO52_001124"
     CDS             complement(27208..28191)
                     /gene="dhaK"
                     /locus_tag="IWO52_001124"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0355405.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(28193..28588)
                     /gene="dhaM"
                     /locus_tag="IWO52_001125"
     CDS             complement(28193..28588)
                     /gene="dhaM"
                     /locus_tag="IWO52_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0355406.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            28742..29062
                     /locus_tag="IWO52_001126"
     CDS             28742..29062
                     /locus_tag="IWO52_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0355407.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(29174..30043)
                     /locus_tag="IWO52_001127"
     CDS             complement(29174..30043)
                     /locus_tag="IWO52_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0355408.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(30139..31443)
                     /locus_tag="IWO52_001128"
     CDS             complement(30139..31443)
                     /locus_tag="IWO52_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0355409.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(31440..31949)
                     /locus_tag="IWO52_001129"
     CDS             complement(31440..31949)
                     /locus_tag="IWO52_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355410.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(32124..34259)
                     /locus_tag="IWO52_001130"
     CDS             complement(32124..34259)
                     /locus_tag="IWO52_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0355411.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(34342..35997)
                     /locus_tag="IWO52_001131"
     CDS             complement(34342..35997)
                     /locus_tag="IWO52_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0355412.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(35999..36313)
                     /locus_tag="IWO52_001132"
     CDS             complement(35999..36313)
                     /locus_tag="IWO52_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0355413.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            36483..36743
                     /locus_tag="IWO52_001133"
     CDS             36483..36743
                     /locus_tag="IWO52_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0355414.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            36757..37071
                     /locus_tag="IWO52_001134"
     CDS             36757..37071
                     /locus_tag="IWO52_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0355415.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT        10814 a         7732 c         6691 g        11951 t
ORIGIN      
        1 aaagattgta acaactgcat attcacacag ttaaattagt gacttcaaaa tgaaataatg
       61 ctcccagcaa aaaattaggc tgggagcatt atttcgttat tctgttcatc aaaaatcagc
      121 gttttgattc catttcatca caagttcctc ctcatttact gcttcatctt tttgctgtgt
      181 ttgaattgta aaaccttgtg aaagatagaa atgaatagct gattgatttt tttgatagac
      241 tgtcaaagtc agtgttttat tggtttcttt tatttttttc attagttgcg taccgattct
      301 ttgttgccgg tattcccttt tcacaaagat tcctgcgata aactgatcaa tcaccccagc
      361 aaaaccaata atttcttgat ctatttctgc taagtaaatc actgcatctg gtaaagcttt
      421 cctaacagat tctacttgat tctcccaata agttgatgga atgaattcat gagaatccaa
      481 gttagcatgc agccaaatcg atagaacaga ttccagttct ttggcagaca attttgttgt
      541 tgtgcggatc attttttctc tccttactta ttcattgatt ttaaaataaa aaccattttt
      601 ttgaagctct tttacaagtt ttcgcccctc aaaaaagtat atcttctctt gttttacttt
      661 tctaatacca aaagaaacag caactttagg atcatcagct gaaacgattc gataagttcc
      721 accaggcaat tgatccagct tgactttccc atgatggtcg ctttgtactg aaaacggcac
      781 accatttcta gtaagtgttt ttttcttatg tttcaaaatg aatgaactac catttaccgg
      841 tttccctgac gaatcactaa taataaatga aaaatcaagc acttttttac ttaatctata
      901 ctgatagatt agtcgtccga taaacactaa cagtatgact acagctatta tcgttcctgc
      961 aagaataaac cattgccgca tggttctttc ctgatttcct tttccttctt ctttttttac
     1021 cgattcgtta taaggaatcc gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata
     1081 cggcgtacat gtaagcaagg taactaagtc ttctccttct gaaatcgtta gaacagacgt
     1141 gtcatctggc agtaccactt gtatattttt aacttgataa gccaatttct cccccaaaac
     1201 agtcaaaata aacgtgtctc cgttttctaa acgatctaat tcggtaaata accgtcgatt
     1261 aggtaaaccg ctgtgagcag agatcactga atgcgtcgaa tttccaccaa gcgggaaaga
     1321 ggttccttgc aagacgcctg ctccattttc aaggatccta ccattgagcc gatcataaag
     1381 tgaaatattt aattggatct taggtatatt gatacttccg atcagccatt cctctaattg
     1441 gctggatgtt gtactttcat ttctgttctt ttcattgaaa ggatcagtac caggattgaa
     1501 tccttggtca gttaatcgtt ggtttttctc ctccattttc tttcgctgca cttcaatttc
     1561 agctgcggtc cgtttttgta cttgttcgat acgtttttga tcaataaaac tatttaaaga
     1621 atccacataa aatggatata aagcgatcag taaccctacg acgatacata ttaataaaaa
     1681 caaacgaaac aaaagtcgac gagactgttt ttgcttactc tgcttcacat gtgtcctccc
     1741 ttgcaagttt ttttgttctt tcttttatta tatgggttca ggaaaaaaat agaaaatgaa
     1801 agccgatgag gttgtgaaaa agctgtcctg catcaagtaa taagtacagc ccaaaaaaat
     1861 agctactcat attttttgtt gggttgggct tattaccgca gatgcaagct tttgaacacc
     1921 gtttaaggaa gaggttgtga aaactgcgtt atgctttgag taataagagc gctagtcaga
     1981 aaatagcttc tcatattttc taggctagtg agcttattac cgagaagcag ctgattgaac
     2041 accgtttacc gagaggtcat gaaaaagcta ctttgtttcg agtattaaga aaaattctgt
     2101 ttcaaaacaa ggagatgcaa caaaacttat gtcgcatcct ctctccttag aactttcact
     2161 tatgtttttc ttgtaaaatt cataaatatt ttcatgaatt ttctaatttc ttgcctacct
     2221 ctcgcatagg atatatgatg aatttatcta aagtttgcaa aggagttcga caattgaaaa
     2281 aatatggccc atttttagca atttttttca ttgctttggc atttctcatg cctcatttat
     2341 tcacacataa actaattttg ggtgctgatg ccccattcca ttacaatcgg ttctatgatg
     2401 ccgcgcaaca aattaaatat ggggatttcc attattttcc ttccctttat ggattccagc
     2461 aaagtggcag gatcgtgaat gccgtttacg gcccattttt cgcttattta caaggattga
     2521 tcgtgttgct tgcaggtagc tggtttcatt atcagattct ttcaaatttc attctttatc
     2581 ttatttcagg attttctatg ctgattttac tgagatatgt caatgttcga aattgggtaa
     2641 gtgtaccagt agccgtttta ttcatgagta cgtattctat tcaatactgg tctatcaacc
     2701 aaggattcac tagctggggg acttgttttt atcctttatg tatgatccct attgctgatt
     2761 tcgtccttca taaaaagttc cctgttatca aagtcgctgt ctcagtggga cttatgacac
     2821 agatccattt actgtcttcg atcatgttag ctttgatgta tatccctttt tatctttatt
     2881 acttttttaa ccagactgaa aaaggaaaag cactgcttca actgattaag tcggtcttgc
     2941 tttattttgt tctgacagca aatatttggg caggattagc tattgtttac agtgggaata
     3001 agattcttcc tccctttgtt aacagagaaa tgagtcaaaa ggcgatagat ctggatggca
     3061 gttattggct ccattaccct aaggcctttc ctatattgat attatcaggt tttttgcttt
     3121 ttattttctt tcaacaaaag aacaccttat tccaaaaaat cgtgttgtac acaagtcttg
     3181 ttttcttttt tctttctact agcttgattc catggacgac attgatcgaa aaaaatgtgc
     3241 cttttattag tttgatccag tttccatttc gatttttcgt gccatttacg ctattgcttc
     3301 tactttacct tgccctttca ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa
     3361 gtatatttgg cattcttatt ttttcagcac agacgatcca gaacttatac cagcatctgg
     3421 aaaagtggga aaatgaaaca tttgtcagtc gacatactta tctattcgat acgccggaag
     3481 aagcaaggaa aacattcttc agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa
     3541 ccacacctga ctatctgcca atctataagg agacaaaaga caataaatac gaccggtata
     3601 acgaagaaat actagaaaac gaaacaactt ttataaaaac acatccaggc ggtcttttga
     3661 caatcaagtg gaaaaacact ggggacaaaa ctgttcatat ccctgttatc gtctacgatc
     3721 ggactgtgct acagcaaaat ggaaaaacat tgactgatta tgaagtaaca gatatcggga
     3781 ccccaatcgt gaagcagcaa aaaggaatca acgagctcac gcttcattat caaacgccaa
     3841 tctatttcta tttcattctt tctcttactt tgattggctg gtttaccttg ctatgtttat
     3901 ttatttatca tagatacaag cttttacgtg cttaggcaaa agggataaat acatccgatc
     3961 aaaaaactct ccaaacggta taatcgtttg gagagtttta ttttaatcag tttttttatg
     4021 ttatatgtta tattttttat tcgttcacat ctaattcgat ttccaccccg tagtgatcac
     4081 tgatgattgg actatcgatc ccatcaaaaa tccgttgata ttttttaacc tgcattcctt
     4141 cagggacgaa aataaaatct atacgcaatt tttctgtatt ttgttcccaa ccatctattt
     4201 tcttttccac agtagctgct tttccttttt cattcgcgac aacatacgaa tcttgaagaa
     4261 gaaggcaact ttctcttaca gtataataac cttgagagtc tactggatta ttgaaatcac
     4321 ctaggaaaaa aagtggtacg cgagtttcca aaaagtagtt ttccaagttt ttccactcaa
     4381 aataaaagcc acttttttta tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt
     4441 cctgatcatc tatctttgtt ttcccaatca agatcttgcg tgtctgataa tcttctttct
     4501 tttgaaattg ggagacggta aggacctgac tttccaatgt ttttttcgta agcagtgcat
     4561 ttccttcttc atatttatca taaccgatgt ggctcatttc ccaactccaa aaatactctt
     4621 ttccgtgttc ctttaaaagc tgaactaaac ggtaggcaaa attatcttta cggataggta
     4681 cttgattctt taccggacag aacttcatta gcttcgcagg ttctacttct tcagattcca
     4741 gtaattggtt gatttcttgt aaagcaatca cttcataatc ttctaacagg atcttatctg
     4801 ctaattcttg caattttttc tctggttctg gctcaagcca gctatgcgtg ttgagtgtca
     4861 tgaatttcat tggttttctc cttgtcttca tttcgaaaca aaagggttgt gacaaaattt
     4921 ttgtcacaac ctctccagca aatagtgttc tcctgctaac tcctcagtaa taagctcaat
     4981 ggaccaggag ctattttttg tttggctgtt tttatcactt ggtgcagggc agctttttca
     5041 cgacctctcc actaaacagt attcaaactg ctgcttctcg gtaataagcc caaccaaacg
     5101 aaaaatagga ggagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt
     5161 tcacaacctc tccttacatt ttaatggagc cgatcacttt accagctgtc actgtaccta
     5221 attgcttgat cactacttct tctactttat cgtttgtaaa tacgatcatg atcgttggat
     5281 ctttttcttc gttttggatc acatcaagat ccatctcagc taatttcgtt cctgttccta
     5341 cttcttggcc ttctttgaca gcaatctcaa aagctggctg attcatttgg actgtatcga
     5401 ttcccatatg gatcaaaact tcgataccat ctttcgtttc cagcccaatt gcatgtttgc
     5461 tagggaaaat acttgtcact tttccagcga tcggcgcaac aacttctctt gatgttggaa
     5521 taacagcaaa gccgtcaccc atcatttttt gtgagaatac cggatctttt acttcagata
     5581 aaggaatcac ttctccagaa gctgcggaaa ctagttcttg ttctatacct aagtattgct
     5641 tactacctgc ttgtccattt cctgtagaaa ccatttctgg ctctggaatc tctgctcctg
     5701 attgcagcag atcttcgata tctgatttca agacatctgc ttttggtcca taaactgctt
     5761 ggactccatt gtctttgacg atcaatccca tcgcccctgc tcgtttccag gcttcttcag
     5821 tccctacttt ttctcgatca tttacactta cgcgtaaccg agtcatacaa gcatcgacat
     5881 ctttgatatt ttcttttcct cccagcaaat gaatgatctg gaccacttgc tgatctgctg
     5941 ttcctgttcc gctgttagat acaacggttt cttcactgtc attgtcgtaa tttccgttac
     6001 gtcccggtgt agcaaagttg aattttttga tcaagaaatt tgctaagaaa tacgtaacaa
     6061 caccaaaagc gatcacacac aacacgaagt tgataagatc tccacctaga cccgctttga
     6121 tagctaacgg tgtccgagtc agcaattcga tattcccaaa tgaatggacc cgcaatggca
     6181 agatatctgc catcgcaaat gcagcccctt ggatcactga ataaatgaca tataatggca
     6241 cagcagcaaa cataaacatg aactccaatg gttctgttac acctgttaga aagacagcca
     6301 acgctgctga aaaatacatt gatttatatc ttgctttctt atcaggatcg acattacgat
     6361 acatcgcaaa agccattccc atcaagatac ctgaagaacc aatcatttgt cctactttga
     6421 aacgagccgg cgtccagtta tgcaacacaa actcatattg cgacatatcc cctgcacctt
     6481 taagattcac taaatcagtg gcccaagcca accataacgg atcttgccca aatacttgtg
     6541 tccccgcctg tgcgcctgat aaaatctcat acgatccgcc aagctgtgta tagttgatag
     6601 gaatcgtcag catatgatgc aatccaaatg gcaatagtaa acgttctaat gttccaaata
     6661 aaaacggtgc tagaatcggt gcgctttctt gtgattctgc aatccataaa ccaaaattat
     6721 tgattccagc ctgtacgttt ggccacacca cagcaagaat caatgccacg attgttgacc
     6781 ataagataac aacaaacgga acaaagcgct ttccattgaa aaatgacaaa gcatccggca
     6841 attttctata attatagtac ttattataag ccattgcccc gacaaatcca gcgatgatcc
     6901 ctacgaatac tcccatattc aaagctggtg cttccaatac acttgtaaaa aatcctttaa
     6961 ccatgatctt cgtaccgaac agagtgtggg taaatgcatc ttcattcgca agcatgtcac
     7021 ttgtgacacc aaagatagcc ccagtgatcc gattgattaa aataaatgag atcccagctg
     7081 caaatgctcc gcctgctctt tcttttgccc aacttcctcc tattgctaaa gcaaataaaa
     7141 tatgaagatt accaataatt gcccaaccaa tattttcaat tactccgccc gttgtgacaa
     7201 gcggcgtcca atcaggattg atcaagggaa ttgatttccc tatactgatc atcaacccag
     7261 ctgccggcat aactgctacc acaaccatta aagcctttcc aaatttttgc caaaactcaa
     7321 aactaaatat ctttttcatt ccgtttagct cctttacgca atcggtttcg ttaaacgtat
     7381 tatagcgtgc gttttctttt ttgtaaatgc tttatttttt atttttctgt ctataccatt
     7441 ctataaaacc tataatacgt gtgttccctt ctataatttt ctcctaaaaa tttatttttt
     7501 taagcttttt tctatgaaat attttctttt gtctattgac ttgtgcaatc ggttgcgttt
     7561 attatgaacg tataaaacag ctcattagga ggagaaaaat gaaacagatc aaacgactat
     7621 ttgctattga tccatggaaa atcagcacga cacaattaga taaaaaaaat cttcgactgc
     7681 aagaatcttt aacaagtatt ggaaacggtt atatggggat gcgcgggaac tttgaagaaa
     7741 cttactcagg ggaccaccat caaggtactt atttggcggg tgtctggtat cccgataaga
     7801 cacgtgtcgg ctggtggaaa aatggttatc cagaatactt tggaaaagtg atcaacgcct
     7861 tgaactttat cgcgatagat atttcgatca acgatcagct gatcgacttg gcttctttgg
     7921 aaccagaaga cttttactgg gaattagata tgaaaaatgg tgtattatct cgtacattca
     7981 caatcactac ttctactaat aaagtacgca tttccttcga acgttttctg agcattgtca
     8041 aaaaagaatc agcttatatc tatttaacta tagaaatgct tgaaggtagc ggaacagtca
     8101 aactcgtatc aaaattggat ggaaatgttc aaaacgaaga cagcaactat gatgaacatt
     8161 tttggaatga gattgatcgt ggaactatag aagaaatcgg ctatttgacg actaaaacta
     8221 ttccaaacaa ctttgggatt gaacaattta ctgttactgc agcaatgcgc catttcgtta
     8281 atggaaaatc agtcatccct gactatcacg aagatacttt agcattacat ggttgcttgt
     8341 ctgcagaatt atctcaaggt gagacatggt cagtggaaaa agaaattatc gttcttacta
     8401 gtagagatat tccagaagcg cagcaagcag aaaaagctgt ctcagaatta acgaaccaga
     8461 caaacaatta tatccaagca aaagaagagc aaacagcggc ttgggcagaa cgatggaagc
     8521 ttgccgatgt ggtcatcgaa ggagacgaag aagcacaaca aggtattcga ttcaatttgt
     8581 tccaactatt ctctacttac tacggcgaag atgaacgatt gaatatcggc cccaaaggat
     8641 tcacaggaga aaaatacggc ggagccactt actgggatac agaagcgtat gctgtccctc
     8701 tttatcttgc tttagccaaa cctgaagtaa ctaagaatct gctgaaatat cggcataatc
     8761 aactgccgca agcaatccac aacgctcagc aacaaggatt aaaaggcgcg ctttatccta
     8821 tggtcacctt cactggtgtc gaatgccata acgagtggga aatcacattc gaggagattc
     8881 accgaaatgg cgcgattgcc tatgcaatct acaattatac gaattacacc ggcgatacgg
     8941 aatatttgaa aaatgaagga ctggaagttt tagcagaaat cgcacgcttc tgggcagacc
     9001 gtgtccattt ttcaaaacga gcaaataaat acatgatcca cggtgtgacc ggcccaaacg
     9061 aatatgagaa caatatcaat aacaattggt atacaaatac gatcgcagct tgggtcttgc
     9121 gttacacgcg agaaaattat ttaacttatc aaaaagaagc tgctatcact atcagcgaag
     9181 aagaactagc caaatggaaa gatatcgcag agaacatgta cttcccagtt gacgaagaat
     9241 taggaatctt tgtccagcat gacacattct tagataaaga tctcatgcct gtatccgata
     9301 ttcccacaag cgatcttcct ctgaatcagc attggtcttg ggacaaaatc cttagatctt
     9361 gcttcatcaa gcaggcagat gtactacagg ggatttattt cttcaatgat acttttacaa
     9421 aagaagaaaa gcaaaaaaac tttgatttct atgaaccaat gactgtccat gagtcttccc
     9481 tttcgccaag tatccattcg attttagcag cagaattagg aatggaaagc aaagctgtag
     9541 aaatgtacca gcgaacagct cgcttggatt tggataatta caataacgac acagaagacg
     9601 gcttgcacat cacttccatg actggtagct ggttgtcgat tgtccaagga tttgctcaaa
     9661 tgaaaactgc ccatgatcag ttgagattcg caccatttct gccaaaaact tggaaagctt
     9721 attctttcca tatcaattat cgcggacgtt tattgttcat ctcagtcaca gatgaacaag
     9781 ttactttcga gttattagaa ggttctcctc tagaaattta cgtctatgaa cagtcttatt
     9841 ctttagcgga tcgtctagaa ataacgctaa gaaaggagga agcaaatgtt taaaggggta
     9901 ttgtttgatt tagacggtgt cattaccgat actgctgaat tccattatcg tgcttggcga
     9961 aagcttggtc aggaaatcgg cattacgatc gaccacacat tcaacgaaca gctaaaaggc
    10021 gtcagccgag aagactcact ctctttgctt ttagcttatg gcggaaaaga acactctttt
    10081 tcaaaggaag aatttgcaga attagcgaaa agaaaaaacg attattattt agagatgatc
    10141 cagacgatcg aaccaaaaga tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa
    10201 gaagaaaaaa tcaagattgc tcttgcttct gcaagtaaaa acgggccatt cctacttgaa
    10261 aaaatgggtc ttacacctta ctttgatgct attgccgacc cggcagaagc aacaaacgga
    10321 aaacctgcgc cagatatctt cctccttgca gcaaaagctg tcggactaaa agcagaagaa
    10381 tgtatcggga tcgaagacgc gcaagccggt atccaagcca tcctctcaag tggcgctcaa
    10441 ccaattggcg tgggacgtgc tgaagacctt ggagaggaga tcccgcttgt ccctgatacg
    10501 acttttctta cgatcgatta cttgaaagaa aagtggcatg accatggata atccagagat
    10561 ctccgtcgta ccagttccag agactgaaat aaagaaaatc acattgaaga acgactactt
    10621 agaagtcgtt cttctcaatt acggtgcccg cctgcaccaa atcttcgcac cagacaaaga
    10681 aggaaaatca gaaaatgttt tattgtctta cgatcgtttc gaagatgttc taacagacaa
    10741 aagctttttt ggagcaactg tcggacctgt agccggaagg atccgggatg cttcttgggg
    10801 tgagcataga ttggagaaaa actgcggatc acaccatatc cacggtggca cgaaaggctg
    10861 gtctttccaa ttttgggaag tagaagtttt caaaactcca tcatcaatag gtgtggtctt
    10921 ttacttaaaa gatgaatttt ctacctatcc aggaccaatc actgctacga tcacttaccg
    10981 tttaacaaaa aacgaattag agatgacaac ctctgctagt agccttgttg aaaccatctg
    11041 caatccgacc aaccatgcct acttcaacct ttcaggaaat ggaacaagag acatctatga
    11101 acatcagtta acggtctttt tagatggtgt gcttgaatta gatcaagaaa aactgcctac
    11161 tggaaattgg acaaaaaaag aaaaactgcc aatcgacttt agaaaatccc caacactgca
    11221 agagatcctt gcatgttatc cagatggact agatgatgta tttcttttgc atcatcctag
    11281 gctttccaaa acttccttac aattattcga gaagcattct ggtcgccaaa tgactatagc
    11341 aacttctaat aaaagtatgg tattattttc tacgactgga ttcgaagctg atttctctgt
    11401 caatggaaaa cagatgcatt caaactacgg attagcaatc gaaccgcaag aaatccctga
    11461 tatcgttcat ttccccaaat ttggatcgat caacctccat cctggtcaag aacggatcag
    11521 tcagacgatt tatcggttct ctgcacaata acaagagaaa ggtgttttta tcatggctat
    11581 taccgtaaaa gatgtggcga aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat
    11641 caacgatcat cctagtattt ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga
    11701 actaggttat gttcctaaca tgactgcccg caatctagga aaacgcattt ccagtgccat
    11761 cggcgtaatc ttaccccctc ttgattcaaa agagcggatc ggtaacccat tttatcttga
    11821 gatcatggaa gcaatcaacg aagaagcgcg gaattatgat atgactacag caatcgctac
    11881 tgctaaaaat tttgaattgc tgttagagaa cgtccagcgt atgcatttac aaaaacaagt
    11941 agatggtttt attctagtgt attcagatag tcaagatact gtcattgatt atttatacca
    12001 aaaccacatc cctttcactt tgatcggaca gccttatcat catgagaacg aaatcgttta
    12061 tgtagataat gataaccagc ttctaggtaa acaagcaaca gaatttttga tccagaatgg
    12121 acataagaat atcctgtttg tgacaaacac gactcatgaa agtttgtatt tcgaacgata
    12181 tttcggttat caaaaagcaa tgatgatggc tggtcttcct gcgttatcca gtgtagtact
    12241 agagcatccg gaggattatc tgtcctttga gaaaacgttg aaagaaaacg aagccacagc
    12301 attggtggtc attgacgata tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg
    12361 ctatgtcatt ccagagacac tctccgtcat tagcttcaac aactcgatct tctcaacatt
    12421 aacacaccct taccttacaa gtatcgatat cgatatctcc gaattaggac gtgtcgctat
    12481 gcaaaagctg cgcgaattga tccatgaaga attgagcagt ggcgtacgtt tggtcatccc
    12541 tcatcgcttg atcaaaagag agacagtcgt tcctttagaa aacccatact aaatagctga
    12601 ctcactaaat acgctgacca tttatcctat ggtcagcttt ttatgtaaaa gaagatacta
    12661 cttctaacac attgtttatc tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg
    12721 tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg gaaataagct
    12781 gaaattcaca aaaatttaaa aagcaatttt cttgagtttc ttcttatttc ttggagttaa
    12841 acgcttctgt cccagcctct tttttccttc cttcaatttt ttacacgtcg acgattcaat
    12901 aaaaaaccgc ctaatattgt aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag
    12961 taatgcaacg cgctaacttt catcgttccc ataatgaatg tgccgctgac tccccacggt
    13021 acgagagaat tgacggtcgc acctgaatcc gctagtgttc gtgtcaaata ggtttttgat
    13081 aagccaacgt catcaaaaga acgcttgaat gtttctcctg gaagaatgat ggacagatac
    13141 tgttctccaa cgatcagatt gattccgata cagctcaagg cagtaaaccc gactaaacga
    13201 gaaggtcggt ccatcttttc ttttaattct tgaacgatgg tatcaacgat cagatatttg
    13261 atcagtaacc cacctaatgc caatgcaagt aaaatcaaac tagcagaacc taacatactc
    13321 atgatcccgc ctcgagaaag cagttcatct agctttggat caggtgtatt tgcgacataa
    13381 ccattcatca agatgtctgc tgcttttccg accgtcaaac ttttctcatg gataaaagaa
    13441 agaacgagag cagctgtcga tcccgctagt aatgtaggga tagccggaat ttttttgatt
    13501 gcccccccaa ataaaataac tagtggaatc aatgtccaag gagagatcca aaagctcgct
    13561 ttcatgtttt ggatcatttc attgatacta gctagatcgg ctgaatgact gttatgtcct
    13621 aatatcgtat aaaaaatcaa cgaaaggacg aatgctggga aatccgtata acgcatacca
    13681 gcgatatgtt cgaatagatt aactcctcca atacccgctg ccaagttggt cgtatctgat
    13741 aaaggcgagc agttattccc taaaaaggca ccggaaacga ctgcgcctgt agtcattgca
    13801 tcattgaatc caagtaaatg accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt
    13861 gtaaatgaac tgccgacagt cactccgaca attgcacaaa caagaaatgc tgtcggtaaa
    13921 aagaaacgca cagaaacaag tttaaatcca taaaccataa tcgtagggat ggttccagat
    13981 aaaatccatg aagcaactaa gacaccaatc aaaagaaaaa tcaggattgg aatgatccct
    14041 ggagtgattc ctttgatgat cccgtcatgg atctcatccc atgtgaactt tttaaatctg
    14101 ccataaaaca acaaacacat gaaagctgca aggatcggga tatgtggcga gagctggaat
    14161 ccgatgatca gtactcctag gagtgccaac aacacgatca atacagcgat actttctttt
    14221 aaagaaatat ttttattcat cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa
    14281 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
    14341 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
    14401 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
    14461 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
    14521 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
    14581 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
    14641 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
    14701 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
    14761 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
    14821 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
    14881 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
    14941 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
    15001 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
    15061 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
    15121 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
    15181 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
    15241 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
    15301 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
    15361 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
    15421 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
    15481 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
    15541 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
    15601 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
    15661 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
    15721 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
    15781 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
    15841 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
    15901 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
    15961 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
    16021 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
    16081 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
    16141 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
    16201 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
    16261 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
    16321 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
    16381 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
    16441 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
    16501 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
    16561 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
    16621 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
    16681 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
    16741 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
    16801 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
    16861 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
    16921 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
    16981 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
    17041 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
    17101 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
    17161 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
    17221 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
    17281 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
    17341 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
    17401 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
    17461 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
    17521 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
    17581 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
    17641 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
    17701 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
    17761 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
    17821 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
    17881 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
    17941 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
    18001 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
    18061 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
    18121 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
    18181 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
    18241 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
    18301 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
    18361 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
    18421 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
    18481 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
    18541 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
    18601 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
    18661 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
    18721 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
    18781 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
    18841 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
    18901 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
    18961 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
    19021 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
    19081 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
    19141 gcccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
    19201 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
    19261 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
    19321 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
    19381 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
    19441 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
    19501 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
    19561 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
    19621 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
    19681 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
    19741 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
    19801 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
    19861 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
    19921 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
    19981 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
    20041 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
    20101 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
    20161 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
    20221 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
    20281 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
    20341 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
    20401 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
    20461 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
    20521 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
    20581 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
    20641 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
    20701 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
    20761 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
    20821 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
    20881 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
    20941 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
    21001 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
    21061 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
    21121 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
    21181 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
    21241 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
    21301 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
    21361 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
    21421 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
    21481 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
    21541 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
    21601 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
    21661 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
    21721 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
    21781 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
    21841 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
    21901 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
    21961 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
    22021 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
    22081 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
    22141 aaaataataa aaaaagtttt ataaaaaggt ataaaacctt tttattaaac tatttaatct
    22201 tgtttagtat atatttttag aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc
    22261 acacatttga aaaagagctt tggtcgtttc gaagctttaa aagatgtctc tttcaccgta
    22321 cattcaggtg aagtcgttgg atttatcgga ccaaatggtt ctggaaaatc cacgacgatt
    22381 cggacacttt tagggattat aaaaaaagac ggcggaacgg caacgatttt tgggcaagat
    22441 gtatgggaag atagtttatc tatccatgaa cgtctttctt atgttccagg cgatatctct
    22501 ttgtggggaa atttgactgg aggagcgatc atcgatctct tcatgaagct tcatggaaaa
    22561 gggagtgctc aaaaaaaaga tgaactgatt caacgtttct ctcttgatcc taagaaaaaa
    22621 gcaaaaaact attcaaaagg aaatcgccaa aaagtgggct tgattgctgc tttggctgtt
    22681 gattcggatc tctatttatt tgatgagcct acttctggtt tagatccttt gatggaaacg
    22741 attttccaag aagaagttga aaaattgaaa gaacagggca aatccatcct actttcttct
    22801 catattctca gtgaagtcga gcgactagcg gataaagtcg tgatcatacg cgaaggaaca
    22861 ctcgatgaag aaggaacact cgatgaattg cgtcatttga ctcgttcgac catcactata
    22921 gaaacagaag cagagaatag tcacttagca caattgaacg gtgtccatga ctttgtgcaa
    22981 aaaggtcacc tcgctcaatt tgctgctgat aacgaagctt tcgatgtgat tttagctgaa
    23041 gcgtccaaat taggtgtgaa aaaatttgaa tctattccac cgactcttga ggatttattc
    23101 atgagacatt atgaaggatg aggaggaagc gaaatgactg ataaattttc acgctggtcc
    23161 accctttttt ctcaaaacct tagccgtgac tggaaaaaga tcctgatatg gctagtcggt
    23221 gtcactgctt tttctggtgg ttttgtccct gcgtttgaag aattgacttc cggacaagga
    23281 ttagctggat tatacgaaac cttgcaaaac ccggcaatga ttgcaatggt cggaccaact
    23341 ccagcttcga atgctgctga gtatacttta ggagcgatgt atgcaaatga aatgttgctt
    23401 ttttgcagtt tgtttgctgc tgtgatctct gttttacacg tggtgagcca aacgagaaaa
    23461 aaagaagaac ttggtctgat tgaacttatt cgttcctttc gagtcggacg tcaagcaaac
    23521 tctttagctg tcatcgctga aagtattctg atcaatttgc ttttagcaat atcgatcagt
    23581 tgtacgatga tttttttcca agcggattcg atcactgcag aaggtgcttg gctttttggg
    23641 ctatctgtag gtagtatggg gattttgggg agtgccgttg cactgtttgc tgcacaactg
    23701 atgccaagtt cagccagttc gaatggtctg tctttagcgt ttttaggtgg actttacctt
    23761 ctacgagcaa taacagatgt agctgattcc tccctttcga tgtggaatcc tttaggctgg
    23821 tcttatttga cctatccatt tacaactaac aactggattc ctttgcttta tttgtttttg
    23881 ttcagtctaa tcttgataat cagttcattt gttttagagg aacatcgaga tttgaacacc
    23941 ggctatatcc ccgaacgtga aggaaaaccg tatgctaaac gttctctttt atccttgcgt
    24001 ggtttattat tctatctaaa taaagggatg acaatcagtt ggcttatcac ttttggtttg
    24061 atgggggcgg catacggctc tatctacggg aatatgcaac agtttttaga aagtaacgac
    24121 ttaatgaaac agatgtttat ccaatctggc gtctcaattg aaaaaaactt tacaagcacg
    24181 atcatggtcg tcttgatttg tctagctgct attttaccaa ttgctagcat caatcgttta
    24241 tttacagaag aaacccacgg acgtatgaat cagctgttcg caacaaaaat ttctaggaac
    24301 caactttatt ggaccaatat gttactagct attctatttg ctatattggc aatcctgatt
    24361 agttctggtg cactagggct ttctgctttg gcttcgatga aggatgcgaa agacatggat
    24421 ctattgatgt ttcttaaagc cgggttcaat ttcctgcctg ccgttttagt gtttatcgga
    24481 attagtggct ttctattcgg atggcttcct cgaatcggta aacttgctta tatctactta
    24541 gcttactcct ttgcactcaa ttactttggc ggaattttgg atttaccaga atggttttcc
    24601 aaaacagctg tccctagctg ggttccacgg atgccaaacg attcttttga tctttccgtg
    24661 ttccttacgt tatcatgtat cagccttgca ctgttcgtcc ttggttctat tggttatagg
    24721 cgaagagact tactagaagg ataataaaaa agcatgtaca atttcatgct agcctccaaa
    24781 tctgggtcaa aaatccaaca tttggaggtc atttttttat ttacaagtct tctaactgct
    24841 ctttatcaat gatccagact tctcgattct ttctttttac ataaccattc tcctctaata
    24901 atttaaattt tctagataaa gtttcggggg tcgtaccgat aaatgcagca agttctttca
    24961 ttttcattgg caggaaaaag tgatccttcc ctgtcgcttt agctaagtca agcagataga
    25021 cagcgactcg ttcttctact ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg
    25081 ttaatttctc aacgttgatt gctaaaagtt tcagactaag ggcgggataa tgcagtaaga
    25141 gttccgagaa atctttttgt ctcaatatgc aaatttccgt tttttctagt gcttcgccaa
    25201 ataaagactc atttggaacg cctagcaact ggctctctcc ctcatatccg cccggctcta
    25261 ttactcgtaa aagctgttcc tttccgctag cagataattg atacactttc atactgccgg
    25321 aggccacgat cactaattga ggttctccat tcggcgcaag aatctgttct cctttttcca
    25381 catagtgatg tttgacaagt gcgtgtactt tttcttgatc ttgctgtact aagtgattga
    25441 acaaaggaac aaatgaaaca cataaatgat catgactcat aatctctcct ccttctaaac
    25501 agctatttct aataaagaaa cataacttcc cttattccaa ctgataattt tcttttatca
    25561 tagcataaac gtcaagcgaa ccagatacca gcaaatagag tggatttctt tttgtccctt
    25621 gctttatttt ccttcttttg agaccttaag taattgattc ctatcaattt tttaaatatt
    25681 tactttttga ataatcaaaa acaaaccttc actcgcaagc cgagagtaag gtttgttttt
    25741 cgcattgctt atttcgtgtt gtctattgaa atttttccgc acctagcttt tgatatagtg
    25801 gtgctagttc ttgtaaggtt tcagcggtta tttgctcaac gtgttcgatt tcttgcggat
    25861 agaagaatag tttacccatt tgaaaggttc ggtctctcat cgcttttttc aactcttctt
    25921 gtttctttgt atcggacaaa tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg
    25981 gttgcggcca tatgtaataa tccttgatat cgactgtttt gctccactct ttcagtatcg
    26041 caagcaactg gttatacgct gcaatgctac catatcgttc ttcgctttta taatgttggt
    26101 acatcaaata gatttggtat tgtttttgat tcaacaaaac tgctagacag gcattctcgt
    26161 cttgacgaga ttcgcttcgg tacgcgctcc aaaaatgact gcgtaaattc cagccattcg
    26221 tccagctttc gattttaggt ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct
    26281 ggagctgaat ctctttccat ttttgccagt gttctttata ttctttcttg atttgttcaa
    26341 tcttatctgt tggatggctc tctttgagct gtttgaatgc gtagattggt cgatcaaaca
    26401 actgataatc tgattttcca agttccattg tttcgcctct ttccgttatc ttttgtattc
    26461 tacctgaatt ttgcgatatg gacaaataga ggttgtgaaa aagctgttct gcatcaagtc
    26521 ataagaacag ccaaacaaaa aactgcgatg agcatcaccg cagttttatt gaacttaagg
    26581 ttgattgatc acatctaaaa gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc
    26641 gatatgtccg attgcacgct ctcctagata ggaagctcgt ccttttttag ctaccaagac
    26701 tttggtgtgt tcttttgcct ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc
    26761 ctctgtgact ggcagccaaa catctaacat cgttttatcg tcagcttctg cctttcctcg
    26821 gctttgaata cccgctgttc cctgctcgat cacttcgcct aatttttctt gagaatcaat
    26881 agaatcgacc tcttttgtcg ctttcatcat gttcatgaat gcagatccgt ataacggacc
    26941 agaagcccca ccgactttcg atatcatcgc catagagaat actttgaatg tatcgctgat
    27001 tgtttcagga tgatcggctt gaaaagcttc ttcataagct gaaacccctc ttcccatgtt
    27061 atttccatgg tctccatcgc caatcggggt atctagatca ctgagatatt gtttgttctg
    27121 attgattgcc tctgcaaatt gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat
    27181 attatcaccc ttttatcttg agatttttta ccatgaaatc gtctgtacat tgctttctaa
    27241 agaatctttc cattgtgtct ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc
    27301 tagtgaggtc ataaaattac cgacttttcg gaaaactaca ttgacgcctc tttcttccaa
    27361 cagagctaaa acatcattca tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc
    27421 gttgactagt acacctgctt cttttggtaa attcgagtaa tcatctaaaa tcttagtaac
    27481 aagttccttt gccagtactt tagaaggctg catcttttct cgacgatagc ctggctcacc
    27541 gtggatacca acaccatact cgatctcatc ttctcctagt tcgaatcctg gtttccctac
    27601 ttctggtaca gttgctgctc gaagcgctag accgatcgtc ttagttgcct gtacgacctt
    27661 ttctcccagt tctttcaact cagctaggga agcaccgttt cttgctgcat cgcccacgat
    27721 tttgtgtaca aggacagtac ctgcaactcc gcgttttcca gcagtatacg tactattttc
    27781 aacggcgata tcatcatcta cgatcacact ttctacttca atatcgtcca tagcagctaa
    27841 ttcctttgcc atatcgaagt tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac
    27901 tccttttcca ctatcggctt ccttaatagc cgtctgaatc tgatctggag ttggagaagt
    27961 aaacacatca ccaagcaccg ctgcacttaa catcccttcc cctacaaacc cggcatggga
    28021 tggttcatgt ccgcttcctc caccagaaac caaaccaact tgttccgctt tctgattctt
    28081 ggctacaaca cgggaagatt ctacacgatg aacaagttct ggatagcttt ttactaatcc
    28141 ttccagcatt tcttcgacaa tattgcttgg atcattcatg atttttttca atttattccc
    28201 atcctttctt tccggcattt tgtgcttctg ttaagatttg ctgagtatca tctgttatac
    28261 cggcagtgat ggctgctgca aaagctcctt ccactaaagg cgcatccacc agatgataac
    28321 gttgctgctg ctcttcttcc aacatgtctt ttgccagttc cgagttcaat accgcgctac
    28381 cgatatccgc aaaaacaaaa taactagctg catcgcttga ttcattgact gcttctacga
    28441 ttttcatcgg gtctgatccg atctcgccat ttattccgcc gagagaaaaa atttgcacct
    28501 cttcggattt ttgcatctgc tcaatcattt ctttaatacc atctgttact ttttgactat
    28561 gagaaataag caaaatactt tttttcacaa gaatcttcct ttcttttaca tagttgctaa
    28621 cgattggttt cgctttcatt aatgaacaat tcaagtatat aataaatttc taattttggg
    28681 aaaatatgtg tagtgcttct tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg
    28741 aatgattaca acaacaacga acagtgtaga gggaaaagcc gtagtagctt ataaagggat
    28801 cgtttttggt gaggtcatta ctgggatcca tgcatttaaa gacttggagg cggggttgcg
    28861 aaatatcttt ggcggccgct caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga
    28921 agcacttgcc gaaatggccg aacgtgcaaa agcattagga gctgatgcga tcattggcgt
    28981 aaaaatggac tatgaagtat taggcagcga caatggcatg ctgatggtca catgtagtgg
    29041 aacagctgtc tccacgagat aagtagggga catctgtttt aataactaaa gagagatggg
    29101 acatgtagtg gcactcaaga attagatttt tcagtctaac ttcgaggtac tctaccttcc
    29161 catcctctct tttttatttc tttttgcgga tgattccgta agctgctgcg actaacacac
    29221 ccgcccctgc taaggaataa ctgacagaag ctgttttttc atttgttttt ggcaaagtct
    29281 gacttgttga tttggatggc tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg
    29341 tcactgattt ttctcctgcc gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta
    29401 agtcagcttt ttttgcttca aaatcattag caactgtttg tgcttcgctg atctttccgt
    29461 ggattgcttg actggccgct gtgatatccg cttgagtttg acttagcttt ccagaagctg
    29521 tttgagcaac ctgatttaat tgctgattga attcttttgc ttgttggatc ttcccgtcaa
    29581 gatctgtttg gacagcttcc gcttttgctt tcgcttcggc aagctttttc tcaagttgtg
    29641 cacggtcatt tcccacttta tttttaagat cagctaaatc ttgatcgatt gctgcttggt
    29701 cttctttcaa tttgtttttt gcagcttcta atgcttcttc gagttctttt cttttatttt
    29761 ccaagtcttg caaatgatcg atttggtcag aagaggtaga actatttatt gatgtatctt
    29821 ctgaagatga aatcgtttct cctgaattat ctgtcggtac attggaagtc atttctgttt
    29881 ccgaggcacc gttttcagca ccagaagagc ttatggttgt tgaaggttct gtttgagcag
    29941 gttcagaagt ttccgcaaaa gctgtctgtc ccaataagaa aatactggaa acagcaattg
    30001 gccagattaa cggcttcatt ctttttttca tcacttttcc catccttact actttttaac
    30061 taatttctta ttataagcaa aaatgtttgg aagaaccata aacacaacag gctgtttgtc
    30121 taaaaaacgg tgcatctttt atttttttgt acgttccggg cttttccatg tcccgcttcc
    30181 ataacctaaa atcgttttga ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac
    30241 tatccagaac aaaagcatat atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg
    30301 atcatctaca atcagcgaag ctagcaactg gaaaaatcct gcgaccattt caaagcagat
    30361 gaacacaaaa ctgaatgtaa aagtaatata aatctgttcg tagtttcctt gatatacata
    30421 atgcccgatt aatccagcaa aaagaactac ggaaatacaa aagaatattg accagacaat
    30481 actcaatgtt tgatcaatga agatgatcgt tcttccaata tgtttaaaag gatggagcat
    30541 cacttttttg aaattcgtca gccagacttc tgttccacct tttgcccaac gttttctgtg
    30601 tcgatatagc atcttcaacg tcacaggtac ttccatgaaa aagataatct caggagcaaa
    30661 aacagataac cagtcattta actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg
    30721 atcttgtctg aatccgccca catcgatcaa ggcttctttt cgatacatcg tatttgctcc
    30781 actgtacgca taaagaccac cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat
    30841 acttgagaat tcaaccgttt gagattttgc aatcaatttg gtacgatttt ggacatccat
    30901 atttgcagtg accgcagcaa tatgtcttgc accagggcga atgaaataat tcacatacct
    30961 aatcagtgca tctggctccg gtaccgtgtc tgcatcattg cttaaaatca atttcccttt
    31021 tgcaaaggca agtccgatat taaacgcgtg ggcttttcct ttattttttt cgatccgaac
    31081 cacacgaaga ttagcgtatt ttttcatcag tctagctaat atttctggtg tttggtccgt
    31141 tgaaccatcg tctgtcacta atacctcata gttgtgataa ttcaatttcg tcatcaaata
    31201 ctcgattgta tattcgatca caatttcttc attatgggct ggcaccatga tcgtgatgaa
    31261 cggttcgaca ctcaagggaa catctaccca ttctttttgc ttatgtttgt ataaaaacac
    31321 ataacaccag acaccaataa accatacaaa tccgcctaaa atcggataag aaacgagaaa
    31381 aaaatacata atagacaaac aagtatctaa gaataaatta cctgtatggg tgattgagat
    31441 catttcagtt ccaccccatt ttctttatac aaatctgcaa tgaatgtcgt atctagattt
    31501 ttctcttcgg agacagaata aaaacggacc gtttctctct cttcttttgt tccaaaacgt
    31561 tccgtataca cttcttcaag taattcttgt ctttttttca atttttcatc ttcatattca
    31621 aaagatttat ttactttttt gtatagattt cgattgttcc acctagtcaa tacaaatgag
    31681 gtaatcagga aactgaaaaa aatcaataca aaaaagccga ttaagaaata cagcattttc
    31741 ttttctgata aaaaagcgta caaggaaaaa tccttggctc tttctggtct cgttaatggg
    31801 aaaaagaccc atataaaagg aagaacaacg atcatccatc ctaaaagagc cactaacgtt
    31861 tgccaaatct ttaaccagta attacctttt gaaaaatact tatctttcac caaaattgat
    31921 gcaacttctt ctcttgttac ttttttcata tcctcacctc acgttttttt gttacgacgt
    31981 aggagtttac catacatttc atttcttttc acataaagtg ttcctaaatt tttcctaaga
    32041 aaaagaacaa aacatttctc atcagcaaat gaaaaatatt ttgttctttt tatcagttgt
    32101 tctttttatt ttttatctct taattatgtt gctctttcca atacgacaat gcttttcatt
    32161 cctctagata tcgccgtgta catttgtctg cgtcttcttt gctcatctcc atcgttttca
    32221 aatggatata agattacatg atcaaattcc agtccttttg ctagactcaa aggaatgatt
    32281 tggcagtttt ctaatccata tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc
    32341 tcttctgttt gggtgataac tgccgctttt tctcctttta gagaaaccaa aatctcagct
    32401 agtattttca acaattcttc agaggatcga aaagagcggt attcgggttc ttttccttgc
    32461 ggtcggatcg atacgatgtc gatcttctct tgatccacag cataggtttt gaaaagctct
    32521 gtgattgctc cgctagaacg gtaactattt ctaagatcat accgatggat cggcagatgg
    32581 tagtcgtcga aacaacgcat aatatcagca aaagtagtgg aagaattaaa gattgcctga
    32641 ttctcgtcac ctactaaagt aaagtgagtt tttggaaata attcgatcaa tagacctaat
    32701 tgtgccaggg tataatcttg tacttcatca atcaatagat agcgaaatgc tggtacattt
    32761 accttttcaa caagtaaatg acgaatagct aatataatga ctgcttcatc aacagtacgt
    32821 gtgggttgcc aagcataagc tgagccatat cgtttttcat aaattctttc aaacaactga
    32881 tgctcatcca cccatgccat ttcttcgatt tgtctggtta tttttcgata ttttttttgt
    32941 aaaagtttcc ttgcataagc tacaatactt tgttccgaat catcagaaat caatttaccg
    33001 aataattcca actgcctatc ttctgtcaac gagagtacct gattctgcac agcaggactt
    33061 tttgcttgtt tcaaaagatg attcttccat tcgctcatca gtgccttttt cgttgcttga
    33121 agcctatcga tcaatttagg atgagcagga actttctcat atattttttc gatcgttttt
    33181 ttagaaagga ctgtttttcc atttttagta agtccacgga taaaattcgg atgatctaaa
    33241 aacaaaggat ctgattgttt tagattatcg ataaactttt tgctacgcaa acgttctgtc
    33301 tgctccgata cattttctcc cgtgatacgt ttgaaataag cctcttcacc ttctatttcc
    33361 tctgctgaaa gctgtgaaac cagctgtatg atggttagat tcagtggatt tctttcacct
    33421 aatgatggta ggacattcga aatatagtcg ataaaccgat gattaggtga tagaatcagc
    33481 aagtcatcgg atgtcatcgt atctcgatat aaatagagca gatacgcaat acgctgcatg
    33541 atagcagagg ttttaccact tcctgctacg ccatttacca agatattttg acttttcaca
    33601 tcccgaatga ttgtgttttg ttccgattgg atcgtagcag tgatagcctt catttcattg
    33661 gtctcatctt ggcctaagac ttctaataat acgtcatctt gaatagctac agacgtatca
    33721 aaaaacttga ccaaacgatc tctttccaaa agaaattgtc gtctttcttt aataaccgta
    33781 tcgattttat tttgacgaac cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt
    33841 tcggcaattg gtgaacgcca atcataaaca agattttcat ctgcatcatc tgtgaaattt
    33901 gctgtaccga tataaaagga ttcttttggc tcattatcca gaaagtctac aacgactttt
    33961 ccaaagtagg gacttttcaa taaacgttcg atctttgcta gctgattttc tgcgtttttc
    34021 tgtttgatat tcatctgatc gatttctcta tttttcattt caatcatcga aaaagtatcc
    34081 aggttatcta agtaactgtc aaaattcaat cgaagctcgc cagccatttc tttcaacgac
    34141 tggtttcctt ctatttttgc cgtatccatc gattcccgca gttcgttttt tttatcaatt
    34201 agcttttgat acacgttctt cagatatgct tcttctaact gtttttgtgt gttgaccata
    34261 cacattctcc tcttttttta aagtggtgga accgattagt ttacctcttt taagagtcga
    34321 atgcaagctt tttaacgagt atcatttgtt aggatatatc agttcttcag gtcggacagt
    34381 ttgatataaa gaaaatgcac catcaaggtt ttttacgtgg tagccagact gcttcaagat
    34441 tctttccgct agatagctac gtaatccgct atgacagctg acgatatatt cttgttggct
    34501 gtctaattca tttaatcggt cccgaagttc attcaaaggg atatgtttgg cttccttgaa
    34561 tttgcccttt tccaattctt ctgctgttcg cacatctaat aaaatttttc cttttgctag
    34621 ttctgtcggc aattcatgcc attgtacggt ttcactaagc ccttctgcaa tgttcattgc
    34681 tgcatatcct agcatgttta ctggatcttt ggctgagcca aatggcggtg catacgtcaa
    34741 ttctaattcc ggtaaatcga agatcgtcaa atgccctttg attgctgttg ctaaaatatc
    34801 gatccgttta tccacacctt ttgccccaac accttgtgca ccataaatct cgccagtctc
    34861 tggatggaag atcaacttca gtaaaatatc tgttgcttct ggataatagc ttgcatgatc
    34921 ttttcctgat acatgaatga cttcaaaaga cagttcgttt tctttggctg ttctttcact
    34981 taaaccggtg gatgcggcag tcattccaaa cgttcgtata attgctgttc caataccgcc
    35041 ttgatttctt cgagaaattc cggaaatcgt gtccgcaact tgtctacctt gacgattggc
    35101 tggtgaagca agagaaataa gtgcatcttt tccagtgatt tgctgtttta caacgatagc
    35161 atcccccacc gcataaatat caggaatatt cgtttgataa cgttcgtcta ctaggatacc
    35221 cccgcgtatg cctaattcga ttccagcggt cactgctagc tggttatcag gacgaacacc
    35281 aatggataaa ataactaaat cagcagggat ggtacgccca tcatccagtc gcaatcgttt
    35341 cccgtggtct tcaaatccag cgacagcatg agaagtaatc acttctactc cttgatgaga
    35401 caattctttt tctataaaag ctgccatttc ttcatccagt gttggaagaa tatgtggtgc
    35461 ttgttcgatc accatgactt ctaaacctct tctttttagg ttttctgcca tttccaatcc
    35521 gatgaatcct gcaccaacga tcacggcacg ctttggctgt ttttctaatg catgcatcac
    35581 tttatcgata tctggtacat ttctaataga aaaaacattg tcggcttctg ttatccctgg
    35641 aatcgatgga acaactggtt ttgcaccagg agataaaacc aatgcatcgt actgttcaat
    35701 gtgtttttga ccctcatgta cgacttctac cgttttgttt tcgggaaaga tggctgtcac
    35761 ttcatgattt ggacgcacat ctaaacaaaa acgagcggat aatgattctg gcgtttggac
    35821 aagaagattt tcccgttcac tgatttctcc cgaaagataa taaggcaagc cgcaatttgc
    35881 aaaagaaaca tacggtcctt tttcaaaaac aacgatttct gcatcttcca ttaatcggcg
    35941 aagccgtgta gcagcagaca ttccacctgc tacacctccg acaataacga ttttcatctt
    36001 atttccctcc tactgttcga tcaaaccatt gattcatgcc accacgtaca ttcactacat
    36061 cataaccaga tttctttaat tctttagctg cttgtttact cctcatccct gattgacaaa
    36121 tcacatagac ggtcttttct ttgtctccat catatctgtt tatactttgt aatggaacat
    36181 ttttagctcc ttttatatgc ccgccccggt attctgcagg tgttctaaca tcaagtaacg
    36241 ctggattttc caaaagcttt tcctttaatt ctgggactgt tatcgatttt attgattgaa
    36301 atagcaaatt cattttctgc acctcattta catttttata aatacctcat ggggtatatt
    36361 aaacaggagc tttattaata tgtcaaccaa aatgactctt catttttttc actacttttt
    36421 atatttgcat aaaataccga taaggggtat tatctaatta taaaaataga ggagaatgtg
    36481 ttatggagat tgataataaa aatatcctga accgtttgaa acgtacagag ggacaaattc
    36541 gtggtatcca aaaaatgatc gaagacgaaa aagaatgtat ggatgtcatc actcaattaa
    36601 gcgctgttcg ttctagtatt gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa
    36661 attgctttga gaaacctgat agtaatccag aagaacaagc ggctaaacta gaacaagcga
    36721 tcaagatgat tattaaaaag taaaggaatg atttcaatga aagtcgtttt ccatattgat
    36781 gaattagaaa aatggtcaga aacagggaaa aacgtaaaaa acctgataaa agcttctcca
    36841 gaaacgacga ttgtcgtatc tgtaaatggc atcgcgatta ccggttatct agactctgcc
    36901 aatgcagaat tcttagattt gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt
    36961 gctaatcata tttcagaaag ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc
    37021 cttgatttag ttgaactgca atcccaaggt tatgcttata tcaagcctta gtcagtagag
    37081 gttgcgacag aagtgatcag cttcaagaaa tttcggctta tttttcgaag aagctacttc
    37141 tggaaaaccg tgaatcaaag gttgtgaaaa cgctgtcctg catcaagt
//
LOCUS       DACEDO010000021        36230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEDO010000021 DACEDO010000000
VERSION     DACEDO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.021"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            232..1500
                     /locus_tag="IWO52_001135"
     CDS             232..1500
                     /locus_tag="IWO52_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAQ0355416.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="IWO52_001136"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="IWO52_001136"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0355417.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="IWO52_001137"
     CDS             3076..3351
                     /locus_tag="IWO52_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0355418.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="IWO52_001138"
     CDS             3357..4124
                     /locus_tag="IWO52_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0355419.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="IWO52_001139"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="IWO52_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0355420.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8154
                     /gene="recQ"
                     /locus_tag="IWO52_001140"
     CDS             6382..8154
                     /gene="recQ"
                     /locus_tag="IWO52_001140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0355421.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8268..8570
                     /locus_tag="IWO52_001141"
     CDS             8268..8570
                     /locus_tag="IWO52_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0355422.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8573..9736
                     /locus_tag="IWO52_001142"
     CDS             8573..9736
                     /locus_tag="IWO52_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0355423.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9762..13190
                     /locus_tag="IWO52_001143"
     CDS             9762..13190
                     /locus_tag="IWO52_001143"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0355424.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13211..14335
                     /locus_tag="IWO52_001144"
     CDS             13211..14335
                     /locus_tag="IWO52_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355425.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14325..14762
                     /locus_tag="IWO52_001145"
     CDS             14325..14762
                     /locus_tag="IWO52_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0355426.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14746..15084
                     /locus_tag="IWO52_001146"
     CDS             14746..15084
                     /locus_tag="IWO52_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0355427.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15203..15763
                     /gene="rsmD"
                     /locus_tag="IWO52_001147"
     CDS             15203..15763
                     /gene="rsmD"
                     /locus_tag="IWO52_001147"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0355428.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15760..16251
                     /gene="coaD"
                     /locus_tag="IWO52_001148"
     CDS             15760..16251
                     /gene="coaD"
                     /locus_tag="IWO52_001148"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0355429.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16241..17284
                     /locus_tag="IWO52_001149"
     CDS             16241..17284
                     /locus_tag="IWO52_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0355430.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17443..18171
                     /locus_tag="IWO52_001150"
     CDS             17443..18171
                     /locus_tag="IWO52_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0355431.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18287..18532
                     /locus_tag="IWO52_001151"
     CDS             18287..18532
                     /locus_tag="IWO52_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0355432.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18529..18747
                     /locus_tag="IWO52_001152"
     CDS             18529..18747
                     /locus_tag="IWO52_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0355433.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18883..19422
                     /locus_tag="IWO52_001153"
     CDS             18883..19422
                     /locus_tag="IWO52_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0355434.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19415..21688
                     /locus_tag="IWO52_001154"
     CDS             19415..21688
                     /locus_tag="IWO52_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0355435.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21726..22772
                     /gene="holA"
                     /locus_tag="IWO52_001155"
     CDS             21726..22772
                     /gene="holA"
                     /locus_tag="IWO52_001155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0355436.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22861..23115)
                     /locus_tag="IWO52_001156"
     CDS             complement(22861..23115)
                     /locus_tag="IWO52_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0355437.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23515..23784)
                     /locus_tag="IWO52_001157"
     CDS             complement(23515..23784)
                     /locus_tag="IWO52_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355438.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(24254..24709)
                     /locus_tag="IWO52_001158"
     CDS             complement(24254..24709)
                     /locus_tag="IWO52_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0355439.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24847..26775)
                     /locus_tag="IWO52_001159"
     CDS             complement(24847..26775)
                     /locus_tag="IWO52_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0355440.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26972..27805)
                     /locus_tag="IWO52_001160"
     CDS             complement(26972..27805)
                     /locus_tag="IWO52_001160"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0355441.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27997..28701)
                     /locus_tag="IWO52_001161"
     CDS             complement(27997..28701)
                     /locus_tag="IWO52_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0355442.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28724..29350)
                     /locus_tag="IWO52_001162"
     CDS             complement(28724..29350)
                     /locus_tag="IWO52_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0355443.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29676..32051
                     /locus_tag="IWO52_001163"
     CDS             29676..32051
                     /locus_tag="IWO52_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0355444.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(32075..32566)
                     /locus_tag="IWO52_001164"
     CDS             complement(32075..32566)
                     /locus_tag="IWO52_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0355445.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32753..33673
                     /locus_tag="IWO52_001165"
     CDS             32753..33673
                     /locus_tag="IWO52_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0355446.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33827..34171
                     /locus_tag="IWO52_001166"
     CDS             33827..34171
                     /locus_tag="IWO52_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0355447.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34361..34861)
                     /locus_tag="IWO52_001167"
     CDS             complement(34361..34861)
                     /locus_tag="IWO52_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0355448.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35389..35472
                     /locus_tag="IWO52_001168"
     tRNA            35389..35472
                     /locus_tag="IWO52_001168"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35423..35425,aa:Leu,seq:cag)
     gene            complement(35796..>36230)
                     /locus_tag="IWO52_001169"
     CDS             complement(35796..>36230)
                     /locus_tag="IWO52_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0355449.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11990 a         6327 c         7491 g        10422 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
     7441 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
     7501 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
     7561 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
     7621 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
     7681 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
     7741 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
     7801 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
     7861 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
     7921 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
     7981 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
     8041 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
     8101 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
     8161 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
     8221 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
     8281 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
     8341 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
     8401 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
     8461 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
     8521 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
     8581 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
     8641 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
     8701 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
     8761 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
     8821 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
     8881 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
     8941 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
     9001 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
     9061 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
     9121 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
     9181 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
     9241 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
     9301 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
     9361 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
     9421 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
     9481 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
     9541 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
     9601 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
     9661 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
     9721 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
     9781 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
     9841 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
     9901 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
     9961 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    10021 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    10081 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    10141 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    10201 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    10261 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    10321 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    10381 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    10441 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    10501 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    10561 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    10621 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    10681 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    10741 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    10801 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    10861 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    10921 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    10981 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    11041 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    11101 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    11161 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    11221 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    11281 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    11341 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    11401 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    11461 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    11521 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    11581 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    11641 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    11701 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    11761 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    11821 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    11881 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    11941 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    12001 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    12061 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    12121 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    12181 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    12241 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    12301 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    12361 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    12421 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    12481 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    12541 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    12601 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    12661 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    12721 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    12781 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    12841 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    12901 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    12961 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    13021 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    13081 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    13141 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    13201 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    13261 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    13321 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    13381 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    13441 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    13501 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    13561 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    13621 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    13681 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    13741 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    13801 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    13861 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    13921 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    13981 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    14041 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    14101 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    14161 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    14221 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    14281 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    14341 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    14401 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    14461 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    14521 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    14581 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    14641 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    14701 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    14761 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    14821 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    14881 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    14941 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    15001 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    15061 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    15121 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    15181 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    15241 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    15301 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    15361 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    15421 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    15481 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    15541 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    15601 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    15661 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    15721 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    15781 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    15841 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    15901 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    15961 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    16021 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    16081 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    16141 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    16201 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    16261 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    16321 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    16381 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    16441 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    16501 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    16561 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    16621 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    16681 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    16741 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    16801 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    16861 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    16921 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    16981 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    17041 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    17101 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    17161 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    17221 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    17281 atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc
    17341 gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg
    17401 aagggacgat tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat
    17461 gatttttact ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata
    17521 ctagcttcac ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca
    17581 agtggttttt tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa
    17641 gaaatacttt cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta
    17701 gaagaggatg ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct
    17761 ggtatctatt ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga
    17821 ttattagaag aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa
    17881 gtcctatata tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc
    17941 tcggaaggaa aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc
    18001 actccatcag aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt
    18061 cgatttagag aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt
    18121 atcggtgaaa agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca
    18181 aaatcgactg gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta
    18241 agagcttggc aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa
    18301 agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca
    18361 tattatgaat acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa
    18421 agatgtactt agctatacag cagataaagt atttattctc attgggacga atgatctagc
    18481 agagcataaa caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag
    18541 ctgaaaaaag actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag
    18601 acaaaacaaa aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa
    18661 gtagcaaaac gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg
    18721 attcgaagaa tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt
    18781 aggatctttt gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg
    18841 agtacttttt aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca
    18901 aaagcatgcg atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct
    18961 tgggaccagt actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt
    19021 ttagaagtag gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga
    19081 gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc
    19141 ttgcgaacga tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg
    19201 acggaaggtt cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg
    19261 ttgcaagctg gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc
    19321 caagcgttga tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat
    19381 ttttcaaaac ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt
    19441 tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac
    19501 tgacaattgg ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga
    19561 aggacgtttt atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag
    19621 gctacaagag tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac
    19681 agatacgatt cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa
    19741 acaaaagctc cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa
    19801 cttccgagga aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa
    19861 gcggaatctt tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg
    19921 gttccaaatt caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca
    19981 gagaaaaaga ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt
    20041 atatattaat tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat
    20101 ttttagggag agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta
    20161 tttagggtgg ctgtattatt tatttcgacg tatcggatgg ccactacacg tcagtattat
    20221 tccgttgagt ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt
    20281 gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc
    20341 caccatggac ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgatttct
    20401 ttttcatgcg ggtggacagc ttagtttttt catgagtttt ttgtttatcc tgatttcttt
    20461 tgatggagat tggttgaaaa aaacggcctc tcacgtcctg tttactatga tcacgatgcc
    20521 gttgattgta tggcattttt atgagtggcc tattttaggt agtcttttta cattactgat
    20581 tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta tttctcacac tcttcaatcg
    20641 ctggctgccg gaaactttac ttttgctgat agaagaggga ttaatcttat ttgagcgaat
    20701 aatcgatttt tcttcagggt cgtctctcat tataggacgt ttgccccttg tgctatctgg
    20761 gatgtatatt ttcggattac tgatttgtat ggataggttg aaagaacaac ctgtgaaaca
    20821 cttgttttat gttggtcttt tttcttttat ttttttgact ttgccatttt tacgcttttc
    20881 ttcaacgatc gcttttatcg atgtcggtca aggagacagt atctttttgc aaagtccttt
    20941 tcgaaaagaa acgattttga tcgatactgg aggtcagtta cactttgaaa gagaaaagtg
    21001 ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc gttccatatc taaaaggtca
    21061 ggggataagt gtaatcgata aactcatctt gacacacgac gatacggatc atgtgggaga
    21121 attacccacg ttatctcagc attttactat caaaaaaatt tatatcggtt ggggcgctgg
    21181 acgaaatgag ccattacaaa ggcatttatc tgaagctcaa aaaaatggta ctgagatcat
    21241 tgaaatcaaa catggggaca ggatcagcgg ttattatgat ctttatgtat tgactccttt
    21301 tgaaaaggga gagggaagaa atgaagattc gattggtcta tggatggaat ataacaatag
    21361 gcgcttttta tttttaggag atttgaatca agcaatggaa aaacaattgc ttttgattta
    21421 tcctaatctg aaagccgatg tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa
    21481 cacagatttt ctttcccata ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa
    21541 tcgttatggt cacccgcaca gcgaagtatt ggagacacta gaagaaaatc agataaaaac
    21601 atggcgaacg gatcaacaag gaatgatcac ctatcgctgg catccagttt ttcatcctca
    21661 tgggctagtg gaaacaatga tagactagta attctcatct gctcatggta ggataatttt
    21721 tgaaagtggt gagtaatttg agtatacaag cagagttaca aaaaatacgt aaagaaaaac
    21781 ttgcatcttg ttacttgatt ttaggaacag aaaagtttct ccaagatcaa gttcggacag
    21841 aaattgtcaa aaaaatccaa ttagagggcg aagatgactt aaatttttta agttttgaca
    21901 tggaaaatgc aagtttagat gaggtagtag ctgaagcaga gacgttgccg tttttcggag
    21961 atcaacggtt agtattcgta gagaatcctt attttttaac aggtgaaaaa gtaaacaatg
    22021 gaatcgaaca aaatactgat ttattgacgg cttatttgaa agaaccatta gaatcgactg
    22081 tattagtatt ctttgcgcca tatgaaaagt tggatgaacg gaaaaaagta acgaaacaat
    22141 tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa tgaaaaagaa gtacgtcaat
    22201 atctgatgaa taccttagag aattcctcaa tcaaaatgga acgaagtgca attgatttat
    22261 tcttgaggtt gaccgactta gatttatcca aaatgatgag agagttacag aaattggtcc
    22321 tttatggaca aaatcaacag caaatcacgg taagagaagt agaagaatta gttcctaaaa
    22381 cgttagaaca taatattttt gacatgaccc aatatatttt aaaagggaag acggaacaag
    22441 ctttgcgttt atacgaagat ttagttatgc aaggggaaga aacaatcaaa attaatgcga
    22501 ttttgctttc tcaattacgt ttatttttac agacaaaatt tcttgtaaag attggttatc
    22561 agcaggcaaa tattgctgaa acattgaaaa tccatccata tcgtgtaaaa cttgccatgc
    22621 aggaagtaaa aaaattcgat gaacatttgc tggtacagtt gtttgatcaa ttagttgaga
    22681 tggattatca aatcaaaacg ggacaaatcg aaaaagaatt aagttttcaa ctattcgttt
    22741 tgcgcacagg gcagttgtta agaaatcgat aagaagaaag acgagggcaa agcatgcggg
    22801 aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa atcggccgaa gccgattctt
    22861 ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg ttagctttgt ttttgtggat
    22921 tagacctttt gattcagcca tatcaatagc tttaacagct tctttatata acgcatctac
    22981 gttgtcagca cctgaagcta cagcatcttc aaattttttg attgcagtac gcatagcgct
    23041 tgtttgagat gaatttttca cattagcgtt ttcgcttgta cgaacacgtt tgattgcaga
    23101 ttcaatgttt ggcattagtt tcacctccga taacttaagt tcttacgcgt gttaccgcat
    23161 aattcaacat tcattattat acctaaaggc agcgtgcttt gcaatatgaa catgacaagt
    23221 ttttttcctt aacagtcttt tattaggatt ttttttcagt aaaaacggat agtacaagct
    23281 gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg tcaaaccaaa ctagattttt
    23341 atttatctat ttaaacaaag tttgaatggg aatgtttggt actaagttat tatataacaa
    23401 agagaaccgt ttgcaaagtt ttttttgaaa agggatacga ttccctgttg ttctttgttt
    23461 ttctattaag aaactgcagt tattttataa ccagtatcct ttgatcagta aaggctattc
    23521 tggtgtaccg attattttaa tgtattcatc gatatttttt atcaacattt tttttatgac
    23581 caactggttc cgacggttcc atttgccact tacttccagc acatatttat ttaggggcaa
    23641 acgtaagata tattttgcca aatagcgatc tttaagcagg ccattgatca cacggtcctc
    23701 aagctgcaag ttgatcccag tcagcatcat tggctgttct tgtaaaacgt acgtatcaat
    23761 taaaagacct tcaaaagttt ccacaagcgt cttcctttct tttcaaagta acctgaaaac
    23821 aggcactaat tcttccattt tgatattcgt tgaagtggat gcgatcattc tttcttttgt
    23881 ccactgtaat ctagtataac aaaaaagagc agaagagcca aaaagaaaaa agagcatcga
    23941 attctatagg aagtctcatt ggcacggcct aaattattac aaaacattga ttaaaaaaag
    24001 cccctgcatc gcaggagcat cccctatatc gttttaatag gtcaatcaaa aagcagtatc
    24061 aaacacattt tgtatcaaat caggaaataa tagataagct aagcccagaa tcaaaagtac
    24121 cgctagaaga aaccagccga ttgttcctat gaaatacttt aatttttcga acattgtatc
    24181 acctcgttgt cagtatatca cggatcagta aattgggggt gttaagaatc tcctaaaata
    24241 gacaaaattt gttctatttt cctaaagcaa atacttgtaa gatacagtaa gtgataccag
    24301 aagcaatgac cggtccggcg gcgattcctc ttaagaagac aacacctaaa atcgtgccga
    24361 aaacaagtgc aacagtgatt tgtggatcag aagcaatcaa tcctacaccc ttggaagata
    24421 agacagccac tagcccaccg caaaaaacag ctacccagcc aataggggat ttaaaggctt
    24481 ctaatagatc acgtagaccg atttctcctg ttgcgatagg gattagaata gccgctgtga
    24541 tgatcgtcac cccaatattg attcctttgc cttgaagcca agggaaaaag gaacgggagg
    24601 ctggtatgag cttcataatc aaaacaaaag ctgccgctat catcagactt tgatttttag
    24661 ctaaaattgc tacaagtaaa ataattccta aaaaaagcca actttccatg aaatatattc
    24721 ctcactttct tcgaaacaat cataacatgt ttttagaaag ttacaaaagt aggaattcat
    24781 ccttcaagac ctattcataa gctgtttttt tcataaaata aacaaaatca taaaaaaaca
    24841 ctttgcttat ttgacagaag acagccgcgc tttgtatagt ggacaagact tacatttttt
    24901 tgaagaatca gaaagatcag gtaaaggtct tagtaaacgc aagccattca gaataaccaa
    24961 tattgtgctt ccttcgtgtc ctacaactcc aagaggtaga tttaaaaatt gcaggaagtt
    25021 agaagcaatc aacaagagaa taacaagaat cgaaaaaata atattttgtt tgatgatccg
    25081 ccgcattttt aaagatagcc gatgactgta gacaaattta tttaagtcgc ttttcattaa
    25141 gacgatgtct gcgatatcca tagcggcatc tgtcccttgc cccatagcga cacctaaaga
    25201 agcggctgct aaagcaggcg catcattgac tccatcacca accatcgcat ttacttggta
    25261 aagttcattc tcttttttga ctagttgtgt tttttcttcc ggagtacagc ttgcatagta
    25321 atgttcaatc cctacttgtt cagcaatggc ctttgctgtg ccagaatggt cgccggttat
    25381 catacttgta tggatgtttt gggatttaaa gtaggaaatt acttgttggg tatttgcttt
    25441 tggaatatct agcagaccta aaacagcaat taactgctga tctttagaaa gataaatcac
    25501 ggttttgcct tgattttcca aacgttcgat catttccgca atctctttgc tgattatcat
    25561 cgtctcagga tcatatgcgt gcttgccaac tttccattgc gcacctaaat aggtagtttg
    25621 taatccaaaa ccagtaattt cttctattgg taagtattgg atttcttcag gtattgtgca
    25681 atcgaaatat tgcaaaatcg catgagcaag tggatgcgtc gttttttgtt ccatagcaac
    25741 aagaagttgt tgtgccaaat ccttttcatc aaagaaaaag ctgtcagtta caactggttt
    25801 tcctttagtg agcgttcctg ttttatcaaa ggcaatcgct tttaattcag aaagcttttc
    25861 taaatggata ccgcctttga ttaaaatccc attttttgca gaatttgaaa gagcagctag
    25921 agaagctggt gtagcagatg cgacaagcgc acaaggagaa gccacaacta ataataccat
    25981 tcctcgataa aagctttcat cccaactcca acctaggaaa aagtggggga gaaggatcat
    26041 tagaggaaca gcaagcaaaa tcaattttac ataattattt tcgattcgtt cgataaatga
    26101 agctgtttga gttggcgtat tctgcgcttc ttctactaat tggataatct tagcaaagac
    26161 agtttcggaa ctcgtttttg ttactcgaat cgtgatggga tttccttgat tcagcgttcc
    26221 tgcatacatc tcatcgccta aatatttttc tacaggcaca gattcaccgc ttaatgcaga
    26281 ctcatcgatc gttgcctgtg tgctttctaa aaagccatct atcggaacag ctgctccttt
    26341 agcgacaaat accaagttat ccacctttag ttgagaaaca tctacttctt gcatttgtcc
    26401 attttctttt aataattggg ctttttgtgg ttgaagattc atcaaagcag taatttcttt
    26461 ttgactttta ttagttgtgt attcctctaa tgcaccgctt aaacaaaaga tgaatgtcaa
    26521 cattgctcct tcaaagtaat taccgatcaa acaggcacca atagcagcta aagccatcaa
    26581 taaatccaca ttcagtgtcc gatcattcca caattctttc aagccttccg atgtttgttt
    26641 atatccaccg atgataatgg cactgatata aaaaagtggg gtgatggtaa gagagaatcg
    26701 gtcgaagata aaaccgaaga tcataaataa aaggcagccg attgttgctg caaaagcttt
    26761 tttctgatta gtcatcgtta ttagacctcc ttgtcaaaac aaggatagca taataaaaat
    26821 gtaattgata ataataacag ctaaatgaga acgataatgg ttatcatttg gtgattaagc
    26881 ttgatgttgt ttttattatt tataatgatt attattaaag aaaataaaaa accagaacat
    26941 ttctcaaata agagaagtgt tctggttttt tctacattgc aataaaccaa taaccaatca
    27001 aaatgatacc aaggataata cgataccagc caaaagcggt aaagtcgttt ttcttcaaat
    27061 agcttagtaa gaatttgata gctaagacgg aaaccacaaa agatacgaat gttcctacaa
    27121 gaagaacaac agtttcttcc attttgaatg aattcccttc agctaaatat ttgacgatct
    27181 tcaagccgct tgcgccaaac atgacaggaa tccctaagaa aaatgaaaac tctgtagcga
    27241 caaaacgcga agcaccaatc aagatagatc ctaggatcgt tgcgccagaa cgggatgttc
    27301 cgggaacaag tgccaagact tggaataacc caacaagaag agctgcttgg tacgtaaagt
    27361 cattcagaga agtccaacga ggtgttttgt ttttgttgcg tttttcgaca ataataaagg
    27421 caataccgta aacgatcaaa acaatggcaa ctggtaagaa cttgtggaaa tttgcttcta
    27481 accaatcatc caaaggcaaa ccaatgatga tggctggtat gcaagcaacg atcactttaa
    27541 accaaagtgt ccacgtatct tttttttcta ccgttgattt acttggagaa aatgggttta
    27601 acttgtgaaa ataaagaacg acgacagcca agatcgcccc taattggata acgacattga
    27661 acatcgacat aaattgtgaa ctggcatcca gtttgatgaa ttcatctgct aggatcaaat
    27721 gaccagtact actgatgggc agccattccg ttattccttc aataataccg agaatcactg
    27781 ctttgataat atttgcaaaa aacataaatt ccttccttcc caattaagat acatcttata
    27841 gtataccgtt attttttgag aaacccaatc tgttttttaa tattaagaaa aacttgagaa
    27901 ataaagcaga taagtacacg aagagaaaaa agaagacaaa gccctcgttc agactttgcc
    27961 ttttatgatt ctctggtaaa aattaaaata tccccattat agaaacgagt cgcgactatg
    28021 tgttttcccg ttatcgatat aagacgtatc attgatgccg tggatagaaa atcgaccgtt
    28081 tgcataagtg acctttgtta cgctgccatt taaaagccca gcacgaacag gctgtttagg
    28141 atcgatcaga ttaagcaaaa aggcgatcgt caaaccatga gatacgacta aaacatttcc
    28201 accaccattt ttttcgcagt gatgggcgat atcttcaaat cctgtccata cacggtctcg
    28261 caatgtttca taagtttccg cccacccagc ggtatctaag ttgtatacag cattagcaat
    28321 ttgttcaaaa gaaacttctg tggtaaacat ttcatcatat gttttaaaat tcaatacacg
    28381 agggatcaca ccccacattt ccatgtcata tccaccttca agagacccga aacaccattc
    28441 tctaatcctt ttttcaataa aataaggaat ttcttttccc ttagaatgtt ctcctagaat
    28501 aatacgggca gtttcgattg cacgaccact gtcactagaa taagcaaatg aaaagtcgac
    28561 attttttaac ccgcgaccta aataatgaat gccttcttgt ccttgctttg tgaggggtgt
    28621 atcacaccag ccttgtactc gttcaattgt attgaacatc gtttttccgt gtcgtacgat
    28681 ataaagtgta gtttctgtca ttttattctc ctcctaagtc gttttaacga ttgaaaaaag
    28741 gatttgtcct tttttcctgt tcaatcgtcg ttgcatcgcc atgtcctgga tagactgcaa
    28801 attcatcagg taatacaaac agctgggtcc gaatactatg aagtaattgt tctaaattcc
    28861 ctgtatacag atcggttcta ccgatacttc ctctaaacag ggcgtcgcca gaaacgacaa
    28921 aatcatcaaa aataaagctg acgcttccta tagaatgccc tggggtaggg acgacgcgaa
    28981 aagacatgtt tcctaaagta taagttttca tttcaaattc atattctgca ggcgagacaa
    29041 taacatcagc aatatcatca tgtctaccga gtccagaaag attcaaaatc ggatcaccta
    29101 gccatttttg ctctagtggg ctgacataaa ctgggatttg ataatacgcg cgtaaaggtt
    29161 ccactgcacc gatatgatcg tagtgagtat gggtaagcaa aatcgctacc ggctgttggt
    29221 ttgttttttt gatttcttgt ttgattcgtt ccgcatcttc tccagggtca atgatcaaaa
    29281 gtgcttcttc gttgtagacc aaataacagt tttcttcaat cattcctgtt tttaatcttt
    29341 cgatatgcat gtgcattcct ccttgatagc caaaacttct ttcatccatt agtataacgc
    29401 attcattgct aggaaacaaa aacaggtact tatttctctt tacttatctg aattatcaga
    29461 ctatatgaaa agtatttgga tattctaaag agaaggaaag taagttttca tagcatttta
    29521 atatgtcctt gttacattag attcatccaa aaatccaaat tctatatcct atatccctgc
    29581 ctaatcagca gggtgttttt ttatgtttgg aaagagagct ctttttgcta aactaggttt
    29641 tttcaggtaa tcttagagag aaagaggtgg tggaaatgat caaacagtca aaaatatctg
    29701 tgcggcactt agtagaattt attttgcgac gtggaagtat cgacaatcgt aaaaaaagta
    29761 accatactgc attagaagga gcgagaatcc accgcaagct ccaaaaagat gcgggagaaa
    29821 actatgaaaa agaagtcttt ttaaaaacaa cagttcaatt agatgaatat tacctgatcg
    29881 ttgaaggccg tgcagacggt attttcaaaa gagacggtat ttattatata gatgaaatca
    29941 aaacctccga accacgattt gaagatttag aaccagaaca agtggaactt ttctttcatc
    30001 aagcaagggt gtatgcctat atctatagtc aagaacatga tttacaagaa ataaatctgc
    30061 aattgactta cttccagacg gcagaagaag tgatcacgag aaaggttgaa catcagacaa
    30121 gagagcagtt agaaactttt tttaaaaagc tgactgaaga ttataaagaa tggcttgttt
    30181 ttcaagaaaa ctggcgcact gtacgcaatg cctctttgat ggctttgaaa ttcccccatg
    30241 atgagtatag gaaaggtcag cgtgaactag cggtagctgc ttataaaacg atccgtacga
    30301 aacagaaatt gtttgtagaa gcacctaccg gaacaggaaa aacaatctct acgttattcc
    30361 ccgctttgaa agctgtaggg gaagaagagg gagaaaagat cttttatcta acggctaaaa
    30421 caataactag acaagtagct gaagatgcta tgacagcatt aaaagataca ggagctgaag
    30481 taaaaagtgt cactttgacg gcgaaagaca agatctgctt tttagatgag acgacctgta
    30541 atccagatca gtgcccatat gccaatggtt attacaatcg gattaatgaa ggtttatggg
    30601 atcttttgaa tcatgagaat cagatcactc gtgaagtcat cgaaacttat gcaagaaagc
    30661 atacactttg tcctttcgaa ctatcattag atgtcagtat ctggtgtgac gtaatcattg
    30721 gggattacaa ttatttattt gatccaactg tttatttaag acgttttttt gaagatgaaa
    30781 aaaacgagga ctatctgttt ttgattgacg aagcacataa tttggtgaat cgttcccgag
    30841 agatgtattc ggcagaacta tcttatgaaa aaacgaaacg aagtaaggaa gctataccaa
    30901 aggaattcaa aaaacttcat cgccgtttca acaaactatt aaaagaattt gattcgatcc
    30961 gagaaatagc aaaagaagat cattgggatt atcatcatca aaaagcaccg gctgaatctt
    31021 tagtaaaggc aggttatcaa ttaagcgaaa aaattaaaga atggctggca gaatttcctg
    31081 agcacccatc acaagaacaa ttgcttcctt attattttga tttgcttcat tttttgaagg
    31141 tcagtgaatt ttatgatgat cactacgaaa caacagtcga aaaaacgtat cgcgatctga
    31201 tagtaaaaga gttttgtatt gatccaagct tgtttcttga gcagtcgttg gataaaggaa
    31261 aaagcagtat tcttttttct gctagttttt caccactctc ttattatcag gaaacactgg
    31321 ggggacaaaa aagtctggct tacaaactgc cgagtccttt cccagaagga aaccagcaag
    31381 ttttcatcgc aaattaccta gagacgactt atcgaaaaag ggaacacagt ctggcaaaat
    31441 tagtagagac gattcatgct tttgctgatg gaaaggtcgg gaattatttc gttttctttc
    31501 cttcttatca atatttagat cttgcggtgg aagcattcag acagcaatat cccgacagca
    31561 atgtgctaat ccaagaaacg gacatgaatg aggaagaacg cgaacgattt ttaacaaaat
    31621 ttcaaacaaa tcccgctgaa acattgattg gattttgcgt acttggcggg attttttcag
    31681 aaggaatcga tttgagaggg gaccgattga tcggcagtat gatcgtaggt gtaggtttgc
    31741 cgcagatgaa tcatgagcag gaactgatca aatcatatta tgatgagaag gaacaccttg
    31801 gatttgctta cgcttatcag cttcctggaa tgaacaaagt cttgcaagct ggtggtcgag
    31861 tgattcgtgg catggcggat caaggaatcg ttgtattggc agatcaacga ttttctagtt
    31921 tttcttatcg acgtttattc ccacaacatt ggcaaacagc aagagaagta cgaagtgttg
    31981 gggaattgga agaaggaata cagcgttttt ggttatcaaa agaagcagta aatcaaaaaa
    32041 acgaggaatg acagacgtca gcctcgtttt ttgattaacg atagttagtg aattgcaact
    32101 cgattggtaa gtcaagttta cgtaataaag caatcacagc ttgtagatca tcacggttct
    32161 tacctgtcac tctaatctga tcttcttgga tttgtgcttt tactttcagt ttttcatttt
    32221 tgatagctgt agtgattttc ttcgcgtttt cacgatcgat tccgttgacc aaatcggcag
    32281 tttgtcgagc tttgccacct agagcgtgtt ctgattcgga aaaatggata tttttgattg
    32341 gcacatttct tttgatcagc ttaccaaata atacatcttt gatctgttcg attttgaaat
    32401 catcatcgct caatacaact agattgctgt tctctaattt gatttctact gtagaatcct
    32461 tgaagtcaaa gcgattggac aattcttttg ttgtttgctg aatcgcattt tttacttctt
    32521 ctgtattgac ttctgataca atgtcgaagc tagcatcttt tgccatagga accctcctga
    32581 tatacatata aatatttacc atacatctat cttatcatag gattagggca gattcttacc
    32641 tgtttgctaa aattcttctc atttgagttc gaagtatttt attttaatag ataaactgtt
    32701 attcttacat ttggaatacc aaagtagggg agaggtgctt gtgagtaaaa ttatgactgc
    32761 gtatcataaa aatgaaagat taaagaaaat agtggttatt ttgataaccg gattgaccgc
    32821 tgcggtcgct ttaaattatt ttttgattcc agccaatgtc ttttctgcag ggatgaatgg
    32881 gatcgctcaa atcatttcct cgcttttata tgaatggttt catatcaaga tagacacagg
    32941 aacatttatt tttcttttga atatccctgt gtttgtatta gggtttctca aagtaggaaa
    33001 acgagcaacg atccttagtt ttgtgaatgt tgtatgtgta tcggtgatga cgatgctgct
    33061 tccagtcgga cagatcactg ataatatttt gatgaatgcc ctagtaggcg gtgttttatt
    33121 aggtcttggc gtaggattat ctttaaaaat gggctttaca actggtggga tggatatcat
    33181 ctctcttgta ctttcacaaa caactggaaa aacagtcgga aactacatgc tgattctaaa
    33241 tggtatcatt gtagtcatcg caggcttctt ctttaactgg gaaagtgcct tgtatacgat
    33301 tatttccatt tatgcaatga ctcaagtaat tgatgccatc cacacgagtc atcaaaaagt
    33361 aacagcaatg atcgttaccg tcaagccaga agaggtaaca gaagaactag caaaacggat
    33421 ggtacgtgga atgacattac tgccgtctgt cggtggctat tcaaaaaaac aagggaacat
    33481 gatcatgatg gtcatcaccc gctacgaaat gtatgatctt gatcagatcg ttcatgaaat
    33541 cgacgacgat gcatttatca atattttacc aactcattca gtatttggcc ggtttgcaaa
    33601 tgaaaacgaa caaaaaatgt atcgaacaac tggcgtattg ccagaaataa aaaatcgttc
    33661 acaaaaaaaa tgaggaatca ggtttatttt agaataaatt taatctatta catctaataa
    33721 gaacattttt ataaatttaa acgagaatct tacttctaaa ttctcattta caagcagcta
    33781 aaaaaagagt atgataaata aaaatcgtgt taaggagtgt cgtacgatga gcgaagcaaa
    33841 ccaagaacgg tctggacaag aaatagaact aatcaaagaa cgtattcttg ctgccttgga
    33901 aatggtgatt gatcctgaat taggaatcga catcgtcaat ttaggcttga tttatgatat
    33961 agaattcaat cctgaaaatg gagaaacagt cattaagatg actctaacaa ctatgggctg
    34021 tccattagca gatattttaa cagaatctat ccatgacgca ttaaaagaag tgccagaagt
    34081 aacgaaagca gaagtcaaac tagtctggta tccagcatgg acgacagata agatgagccg
    34141 ttatgcacgg attgcgttgg ggatacgata gaaatcttgg ctgactagct atgagaagca
    34201 aataggaaaa acaaaaaagc cttttatcct ttctagaaga aaagagataa aaggtttctt
    34261 tgtttataaa attttgtctg ctatattata agttaattaa ataaatgtgt tctattgata
    34321 aaggacattc aaatcccttt ccttttggaa tccttctttt ttacatcagt ttgattttcg
    34381 tatagtattc tttctcatct tcacgataaa tcgcagcccc atttttttgt tgtgctttaa
    34441 gactgccaag atttttttta tttacagaca aaagtgcctc atcaatgccg attttttttg
    34501 cctcttgtag tactaatgct aatccttgtg tggcatatcc ttttttacga taattagggg
    34561 agatgccgta accaatatgg ccagcgccgt ttcttaatgt ttcgttcagt ttatggcgca
    34621 aattaaaaat tcctacatac tcatgttcat cactttctaa aataaagacg gttgcgggaa
    34681 cgtaactctc aggaagatcg attcctaaag acgttttttc gcattttttt acataatcta
    34741 aaaattcagc aaatgtatag ccataagcat tgttgataaa tccactctca tttggtccaa
    34801 agctagtttg tagctgataa gctttcttta aatctgcttc ccatagtttg atcagtttca
    34861 tcgtattcac ctctttaagt aaccttattc atttgttttc tactatagca ctattgttgt
    34921 aaatagccat gcctattttt atttattgtg tcatatcgta ttaagaatca tcgaacaata
    34981 caaatatgaa ttacaataaa aaaattattt gaagttcgtt cattcgttac ttatgatctc
    35041 tagaagaaag aaaacgtgaa ttacttcagc gtctagaatt ccagaaggat agtaatcaac
    35101 agaagaaata atcaatatcc aaatgaaaga agatagctga atcgagaaaa agaggaaaaa
    35161 agaaatgaag aaaaaaataa aatagttact aatcccagag tcgttcgttt aacattgaaa
    35221 tcgatcatat ggataaaaca gattttttca ccaaccctca atagatgatg gagttgctcg
    35281 gatgaattat ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa agaacaaaaa
    35341 tatgttcttg acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc ggctatggcg
    35401 gaattggcag acgcgctagc ttcaggcgct agtgagggtg acctcgtgga agttcgagtc
    35461 ttcttagccg cataatttgt ttttttataa agagaaggta cacctggagc gatgagaaag
    35521 aaaataacat attatctaaa agaatatagg aaaaatttta aacaacggaa ccagattcaa
    35581 ataaatcaaa aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag attgattcaa
    35641 atagtagatt tatagattct gatacaagaa atactggcaa acgatagtta aggaataaag
    35701 actaaactta aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac ctcttactat
    35761 attttgaatg tgtttaaacg ttcaaataat gaatcttagt accaaccgtt tgcttcccag
    35821 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    35881 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacggccg
    35941 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    36001 tttgcagagc ttgtacttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    36061 gtttcagcaa caggcgctgc ttgtgtttcc gtttgaaccg tttcttgtac aacaggttgt
    36121 tcagcttgaa caggttcagc ttggactggt gcttcttgaa cggctgcttg aggttcagca
    36181 gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DACEDO010000022        34862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEDO010000022 DACEDO010000000
VERSION     DACEDO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.022"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IWO52_001170"
     CDS             complement(59..187)
                     /locus_tag="IWO52_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0355450.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWO52_001171"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWO52_001171"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0355451.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IWO52_001172"
     CDS             complement(1674..2729)
                     /locus_tag="IWO52_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0355452.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWO52_001173"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWO52_001173"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0355453.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWO52_001174"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWO52_001174"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0355454.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWO52_001175"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWO52_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0355455.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWO52_001176"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWO52_001176"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0355456.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWO52_001177"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWO52_001177"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0355457.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IWO52_001178"
     CDS             10491..12050
                     /locus_tag="IWO52_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0355458.1"
                     /translation="MKKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IWO52_001179"
     CDS             complement(12161..14092)
                     /locus_tag="IWO52_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0355459.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWO52_001180"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWO52_001180"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0355460.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IWO52_001181"
     CDS             complement(15021..15773)
                     /locus_tag="IWO52_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0355461.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IWO52_001182"
     CDS             complement(15932..16141)
                     /locus_tag="IWO52_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355462.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IWO52_001183"
     CDS             complement(16152..17480)
                     /locus_tag="IWO52_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355463.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IWO52_001184"
     CDS             complement(17845..18432)
                     /locus_tag="IWO52_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0355464.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWO52_001185"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWO52_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0355465.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IWO52_001186"
     CDS             complement(20012..21301)
                     /locus_tag="IWO52_001186"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0355466.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IWO52_001187"
     CDS             complement(21455..22387)
                     /locus_tag="IWO52_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355467.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IWO52_001188"
     CDS             complement(22544..23203)
                     /locus_tag="IWO52_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0355468.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWO52_001189"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWO52_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0355469.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IWO52_001190"
     CDS             complement(24244..24534)
                     /locus_tag="IWO52_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355470.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IWO52_001191"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IWO52_001191"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0355471.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IWO52_001192"
     CDS             complement(26821..28176)
                     /locus_tag="IWO52_001192"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0355472.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IWO52_001193"
     CDS             complement(28200..29369)
                     /locus_tag="IWO52_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355473.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IWO52_001194"
     CDS             complement(29366..30253)
                     /locus_tag="IWO52_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0355474.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTRAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34134)
                     /gene="nifJ"
                     /locus_tag="IWO52_001195"
     CDS             complement(30442..34134)
                     /gene="nifJ"
                     /locus_tag="IWO52_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0355475.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34333..34854
                     /locus_tag="IWO52_001196"
     CDS             34333..34854
                     /locus_tag="IWO52_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0355476.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9407 a         7570 c         6268 g        11617 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atgaaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccgtcat tgatatttaa
    27541 cccgtctgga tttgtgccca ttgtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaactt catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatc
    29221 ggcacccccg taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatt agttctctac
    29461 gcaagattgc caaatattct tgctgggata agccagccat cagttcatta tctaacgtga
    29521 tgctgacgtc cccggtttct tcagagacaa cgatggtaat cgcgtcactg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attctttagg gatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc cccagtgata tctgcatcta
    29761 aagcaatccc tgtctcaata tactcatcta atccagtatg acgttcgata gtgatcaacg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgctct tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tatgctcgta tttttcttga gaaaaacgca taattttcaa
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct agcagctctt ctttcttctg cttcaaggtc
    30541 cgtcgttttt tcttttctag tcttaacggg tacatcgata gattcctctg gttcaagtcg
    30601 tttcttgatt ttttctaatt ctcccgtcag tgcagcatag ttgtaaaaac gtgttttagc
    30661 gtcgtggaca gtcttttcaa ataaagcttt tgcaagattt ggatttgcgt tatctagcga
    30721 ggaaaagcga acttgtttcc tcatgaaatc aggcatagca gagaaatcag gttttttgta
    30781 gtccaaagtc attgggttct tgtttttttc gaccattgta gggttatatc gatataatga
    30841 ccaataacca gaagaaacag cttcttttgc ttctttaagc gattctttca ttccgccaag
    30901 caagccatgt gtgatacaag gcgtataggc aataatcaga gaaggaccag gatacttttc
    30961 tgcttcttca aatgccttga tcgtttgcat ttgattggca cctgaagcaa tttgcgccac
    31021 ataaacattt ccatatgtca tcgccatcat tcctagatct ttctttgaag catactttcc
    31081 actagccgaa aatttagcga ttgccgaagc tggtgttgct tttgaggttt gaccgccagt
    31141 attggaatag acttcgttat ccaaaacaag gatattcacg tctgctccgc tggcaagtac
    31201 atgatcgatc ccgccaaaac caatgtcgta agcccatccg tctccaccaa tcatccattg
    31261 gctgtttttt acgaatagat cctttctctt gtatatatcc tccagttcag ggaaaattga
    31321 tcgttcgctt tcgatcgcag cttgcagttt tgctgctctt tgctgcgttc cttcactctc
    31381 aaacatgtgt actaaccagt cttccatcaa ttctcgtagt tcttctgata cttttggcaa
    31441 tacttctttg attgaacttg ccaaagcctc gcgacgagtc tgtgccgcta gaagcatacc
    31501 ataaccaaat tcagcattat cttccaataa agaatttgac catgcaggcc cttgaccaca
    31561 ttcattggtc gtataaggag aagcaggaga agcaccgccc caaatagaag aacagcctgt
    31621 cgcattcgcg atcatcattc gatcaccgaa catttgtgtc aataatttta cataaggtgt
    31681 ttcgccacag cctgagcaag cgcccgagaa ttcaagaagc ggttgattga attgtgtaga
    31741 caagacagta ttctttcctt tgactggatt tgctttttgt cttaatgtca ttgcaaaagc
    31801 ccaattcaca gcttgttctt tctgttcttc ataaggcttc atcactaaag ctttttcttt
    31861 tgcgggacaa gcctctacac acagaccaca acctgtacag tcttccactg aaacttggat
    31921 acggtatttt acgccgtctg cgcctcgcat ttctctcacg ataaatcctt ctggcgcttc
    31981 ttccatttcc tcttcatctg ctaaaaatgg acggatggca gcatgaggac aaacaaaggc
    32041 acattcatta cacatcgtac agcgatcact aatccactca ggtacttcta aagcgacgcc
    32101 ccgcttttcc atagaagccg ttcctaaagg catttcgcca gttgtcattc catttgctac
    32161 aagtgtacca acagagagtt gatttccttc ctgtgcattc actggttcaa gtatttcttg
    32221 tatataacca gtgttccttt tattgatttt ttcaggcatc tccaacgttc tccaatggtc
    32281 tgggatcgcg acttgatgaa gcagttcggt cgtccgatcg attgcttgta tgtttttttc
    32341 tacgatagcc attgatttat gtccatagct tttcagggct tcttctttta gcagcggaag
    32401 cacctcatca aaaggaatga tgtcagctaa tttaaaaaaa gccgtttcca ttgccgtatt
    32461 gatccggcgt cccaaaccga cttcttgggc caatctcatg gcattgatcg tatagaatct
    32521 aatgttattt tcagccaagt aacgttttaa ttttaagggc agatgcgttt ctaattgttc
    32581 gtcgctccaa agagtattca ataaaaaaat gccacctggc ttcaatcctt tgaccaagtc
    32641 atagctatgc aggtaagctg gtgtgtgaca ggcaacaagg tccgcatgct cgacaagata
    32701 cgctgaacgt atcggcgtat ctccaaaacg aagatgagag acagtcagac cgccagattt
    32761 tttcgaatca taataaaaat agccttgcgc atatttatcg gtgtgatctc caataatctt
    32821 gatagctgat ttatttgctc caactgttcc gtcagatcca aatccccaaa acttggcctg
    32881 gaatgtttgc ggttctgtca aatccagact ttcctttggt tccaaagatt gataagtcac
    32941 atcatccacg atccctatgg taaaccgttt tttgcgtatg cttgggtctt ttttcaattc
    33001 atcaaaaact gctgagattt ggtcgggagt cacatcttta gaccccaatc cgtagcgacc
    33061 accaataaca gccgggcgaa gttcagaatc atataacgca ctttgaacgt ctaataagag
    33121 aggttctcca cctgcccctg gttctttact tcgatcaaga acagcaattg tttttactgt
    33181 atgaggcaac ttttgcacga atgtttccac tggaaaagga cgataaagat ggatattcaa
    33241 aaaacctgtt ttccgtccgt ttgcattcaa gtaatcaatc gtttgttcaa tcgtctgtgc
    33301 aactgatccc atagaaacaa tgacttcttc cgcatcgtct gctccgtaat aagtcaccaa
    33361 gtcataagaa gttcctcgta atcggttgat ctctttcata tatttttgaa cgatttcagg
    33421 taatttatca taataggggt tgatcgtttc ccgttgttga aaatggatgt ctggattctg
    33481 gttcgtacca ctaacagtcg gatgattggg attcatccct cgtttacgaa atgaagctaa
    33541 tttctcctga ttgatcagag gagctaattc ttcatattcc aaaacttcga ttttttgaat
    33601 ctcgtgactc gtacggaatc catcaaagaa attcaaaaaa gggacttctc cttcaataga
    33661 agcaagatgg gcaacaggtg ctaaatccat cacttcttgt acactgcttt cagctaacat
    33721 agcaaatcct gtttgtcttg cagccatcac gtctccgtga tctccgaaaa tattcaacgc
    33781 atttgtcgtc actgcccgac tagcaacgtg gaaaacactt ggcagtagtt ccccagcaat
    33841 cttgtacata ttcggtatca tcaaaagcag cccttgtgaa gcggtatatg tagttgtcag
    33901 tgcaccagtt ttcaatgatc catggaccac accagctgct cctgcttcgg attgcatttc
    33961 tactactttt actggttgac cgaatacgtt ttcttttcct gctgctgacc attgatctac
    34021 taattcagcc atggtcgaac tgggtgttat tggatatatt gcagcaatct cggtaaatgc
    34081 ataagaaata tatgcggctg ccgtattgcc atccattgtt ttcatttttc gcatgattca
    34141 tcatccttgt tcatattctc tcttattatc tgttccatta taacgtaaaa catgccattt
    34201 ttttctttta tttataaaaa tattttacat gtatctattt gaattggtca tctaattttt
    34261 agacaaacag tctaaatcgt tgcctttttt tgtcagacat gtataaacta taagcgaaag
    34321 aggtgtttta acatggatat atcagtgatt gatgcaacga aaacaaatac acaaaaagga
    34381 atcctttatg gttcatcgaa tgctcctaaa aaaatggtcg aattcatcaa cttagcttgt
    34441 ccgtattgcc gacaatggtt tgaagaatct tatgagctat tagaagaagc agtccaaagc
    34501 gggcaattgc agcgagtgat caaattgttc gataaagaaa aagaaagctt acagcgaggg
    34561 aacgtgatgc accggtatct cacaatcagc gatggccaaa aagcgatcaa agagatcaaa
    34621 caaatattcg atactcaaga cgagtggaaa catctttctc tccaagaagt agcggatttt
    34681 gcagtagata aactaaagct gacagaacaa aaagacgaac agctgagcca agctgtcatc
    34741 aacgaagctg agcaagcaca tattcgattt gttccaactg tcatcttggg taaagaaatc
    34801 tttgatgaat ctatttcaat agaggaatta aaagaattga tccaaacaaa ataaaaaaat
    34861 aa
//
LOCUS       DACEDO010000023        34454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEDO010000023 DACEDO010000000
VERSION     DACEDO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.023"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            85..972
                     /locus_tag="IWO52_001197"
     CDS             85..972
                     /locus_tag="IWO52_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0355477.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IWO52_001198"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IWO52_001198"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0355478.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IWO52_001199"
     CDS             2274..2720
                     /locus_tag="IWO52_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0355479.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IWO52_001200"
     CDS             2993..4345
                     /locus_tag="IWO52_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0355480.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IWO52_001201"
     CDS             4516..4803
                     /locus_tag="IWO52_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355481.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="IWO52_001202"
     CDS             5029..5472
                     /locus_tag="IWO52_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0355482.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="IWO52_001203"
     CDS             complement(5478..6662)
                     /locus_tag="IWO52_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0355483.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="IWO52_001204"
     CDS             6930..7628
                     /locus_tag="IWO52_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0355484.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="IWO52_001205"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="IWO52_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0355485.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="IWO52_001206"
     CDS             complement(8438..8971)
                     /locus_tag="IWO52_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355486.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="IWO52_001207"
     CDS             complement(9268..11649)
                     /locus_tag="IWO52_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0355487.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IWO52_001208"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IWO52_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0355488.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="IWO52_001209"
     CDS             12350..12889
                     /locus_tag="IWO52_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0355489.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="IWO52_001210"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="IWO52_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0355490.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="IWO52_001211"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWO52_001211"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="IWO52_001212"
     CDS             13996..15162
                     /locus_tag="IWO52_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0355491.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="IWO52_001213"
     CDS             15208..16704
                     /locus_tag="IWO52_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0355492.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="IWO52_001214"
     CDS             16785..17468
                     /locus_tag="IWO52_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0355493.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="IWO52_001215"
     CDS             18113..19303
                     /locus_tag="IWO52_001215"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0355494.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="IWO52_001216"
     CDS             19405..20358
                     /locus_tag="IWO52_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0355495.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="IWO52_001217"
     CDS             20427..21776
                     /locus_tag="IWO52_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0355496.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="IWO52_001218"
     CDS             complement(21959..22282)
                     /locus_tag="IWO52_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355497.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="IWO52_001219"
     CDS             22411..23574
                     /locus_tag="IWO52_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355498.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="IWO52_001220"
     CDS             23644..24993
                     /locus_tag="IWO52_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0355499.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="IWO52_001221"
     CDS             complement(25038..25469)
                     /locus_tag="IWO52_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0355500.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="IWO52_001222"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="IWO52_001222"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0355501.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="IWO52_001223"
     CDS             28699..29295
                     /locus_tag="IWO52_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0355502.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="IWO52_001224"
     CDS             29417..30565
                     /locus_tag="IWO52_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0355503.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="IWO52_001225"
     CDS             complement(30603..31016)
                     /locus_tag="IWO52_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0355504.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="IWO52_001226"
     CDS             31095..31526
                     /locus_tag="IWO52_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0355505.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="IWO52_001227"
     CDS             complement(31625..31858)
                     /locus_tag="IWO52_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0355506.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32041..33711
                     /locus_tag="IWO52_001228"
     CDS             32041..33711
                     /locus_tag="IWO52_001228"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0355507.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="IWO52_001229"
     CDS             33858..34244
                     /locus_tag="IWO52_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355508.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..>34454
                     /locus_tag="IWO52_001230"
     CDS             34231..>34454
                     /locus_tag="IWO52_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355509.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIA"
BASE COUNT        11438 a         5926 c         7188 g         9902 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtggaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaaat aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaagaac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg caga
//
LOCUS       DACEDO010000024        33915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEDO010000024 DACEDO010000000
VERSION     DACEDO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.024"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1041..1706)
                     /locus_tag="IWO52_001231"
     CDS             complement(1041..1706)
                     /locus_tag="IWO52_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0355510.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1832..3868
                     /locus_tag="IWO52_001232"
     CDS             1832..3868
                     /locus_tag="IWO52_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0355511.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3872..4510
                     /locus_tag="IWO52_001233"
     CDS             3872..4510
                     /locus_tag="IWO52_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355512.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4538..4966
                     /locus_tag="IWO52_001234"
     CDS             4538..4966
                     /locus_tag="IWO52_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355513.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5423..6334
                     /locus_tag="IWO52_001235"
     CDS             5423..6334
                     /locus_tag="IWO52_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0355514.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6551..6988
                     /locus_tag="IWO52_001236"
     CDS             6551..6988
                     /locus_tag="IWO52_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355515.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7083..7967
                     /locus_tag="IWO52_001237"
     CDS             7083..7967
                     /locus_tag="IWO52_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0355516.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8191..11358
                     /locus_tag="IWO52_001238"
     CDS             8191..11358
                     /locus_tag="IWO52_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355517.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11378..11923
                     /locus_tag="IWO52_001239"
     CDS             11378..11923
                     /locus_tag="IWO52_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0355518.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            11951..12406
                     /locus_tag="IWO52_001240"
     CDS             11951..12406
                     /locus_tag="IWO52_001240"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0355519.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12403..13161
                     /locus_tag="IWO52_001241"
     CDS             12403..13161
                     /locus_tag="IWO52_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0355520.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13247..13987)
                     /locus_tag="IWO52_001242"
     CDS             complement(13247..13987)
                     /locus_tag="IWO52_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0355521.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14048..15301
                     /locus_tag="IWO52_001243"
     CDS             14048..15301
                     /locus_tag="IWO52_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0355522.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15623..15850
                     /locus_tag="IWO52_001244"
     CDS             15623..15850
                     /locus_tag="IWO52_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0355523.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            15973..16299
                     /locus_tag="IWO52_001245"
     CDS             15973..16299
                     /locus_tag="IWO52_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0355524.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16367..17080
                     /locus_tag="IWO52_001246"
     CDS             16367..17080
                     /locus_tag="IWO52_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0355525.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17390..18241
                     /locus_tag="IWO52_001247"
     CDS             17390..18241
                     /locus_tag="IWO52_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0355526.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18362..20227
                     /locus_tag="IWO52_001248"
     CDS             18362..20227
                     /locus_tag="IWO52_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0355527.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20241..21683
                     /locus_tag="IWO52_001249"
     CDS             20241..21683
                     /locus_tag="IWO52_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0355528.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            21791..22492
                     /locus_tag="IWO52_001250"
     CDS             21791..22492
                     /locus_tag="IWO52_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0355529.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22664..23143
                     /locus_tag="IWO52_001251"
                     /pseudo
     CDS             22664..23143
                     /locus_tag="IWO52_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            23154..23660
                     /locus_tag="IWO52_001252"
     CDS             23154..23660
                     /locus_tag="IWO52_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAQ0355530.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            24071..24646
                     /locus_tag="IWO52_001253"
     CDS             24071..24646
                     /locus_tag="IWO52_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0355531.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24646..25086
                     /locus_tag="IWO52_001254"
     CDS             24646..25086
                     /locus_tag="IWO52_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAQ0355532.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            25102..25803
                     /locus_tag="IWO52_001255"
     CDS             25102..25803
                     /locus_tag="IWO52_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0355533.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(26301..26726)
                     /locus_tag="IWO52_001256"
     CDS             complement(26301..26726)
                     /locus_tag="IWO52_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355534.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            26957..28282
                     /locus_tag="IWO52_001257"
     CDS             26957..28282
                     /locus_tag="IWO52_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0355535.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            28284..30584
                     /locus_tag="IWO52_001258"
     CDS             28284..30584
                     /locus_tag="IWO52_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAQ0355536.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            30591..31562
                     /locus_tag="IWO52_001259"
     CDS             30591..31562
                     /locus_tag="IWO52_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0355537.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            31584..32981
                     /locus_tag="IWO52_001260"
     CDS             31584..32981
                     /locus_tag="IWO52_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0355538.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            33137..33760
                     /locus_tag="IWO52_001261"
     CDS             33137..33760
                     /locus_tag="IWO52_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355539.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
BASE COUNT        11392 a         5544 c         7069 g         9910 t
ORIGIN      
        1 acatcaaggt tcctagcctt tttataataa aatgaagcga aaataatttc ttttaaagag
       61 ccgagattat cagctatata agagtgaatt tgatgcaaaa aacagagccg aaacagtctt
      121 ggtgttcgta gctctgctta tatgtttcga atttaagaca aagagttggc aaaagatgtt
      181 gataaggaag ataagataac tgtgttatat aaactaggta actaactgta acaagctaat
      241 tttaagagat tcttattaga agacgtttag gaatataact aagaaaatga ttatacaggt
      301 aattgtcatt tcaataatgt tttctaattt tcgcgtcttt gttttaagaa aaaggccaga
      361 atcagttgga aaaaagttcg tacgatagtg aagaggggca aaatttccta ctaatattcc
      421 cactaacaag agataaaacg gccaggttgg tgtgattcct ctataaaaaa atgcccatgc
      481 taccgaaatc ccatagatta acaactgaac aatttttaca atgaaaaaat tgttctgcca
      541 ttactcaaag tctcttcctc cttgtatcgt ttatttgttt cattttatca ttatgatagg
      601 cgcttgaaca atccattttg tattttattg agtactcttg gataaactac cagcgattat
      661 catatttatt tatgttactg taagccatca tataaaagta aatgagcaga tcatagaaat
      721 ataaaaaaga actaaaattg atctaaatta tgtgcagctt gactacgtac caatctatat
      781 tacttactag attggtcgtt aatctattcg gatcgaatga gcaaaatatt tttgtgaatg
      841 tccatccttg attatgattc caaaaagaat cattgggagg ctgtggcgta aatattatac
      901 tataagccag aaatacctat cattctgaac ttttttattt aggaactttc atctcaaggc
      961 tttttcaatt aatttgtacc cctgtttttt ggataagatc atgtgcattg attttttcta
     1021 tctctacgtt catctcctac ttaatgccta tctggctcct gagggcttct ttttaaggcg
     1081 tcgtcatagt cttggctgag gatttcttgg taatctgagg gaatggcttc ccaagtgagc
     1141 aggcgaacgt tcatgacgtg ttttcctttg tgtttgatta cagcatggtt tttctctttt
     1201 tctacctctc cagcgtgaaa agacagccat gtaccatggc caaagatatc attacatacc
     1261 caacgttcga tggaattgtc cacgtatcct acagggagtg tggtatagcc gatttgtggt
     1321 ttcaaaaaag gattgaatcg atcgatcgtt tcatttttgt attgataatt gataagaaaa
     1381 agatagacca aacaaaagac gagcagaaag aaagatagtg tctttttgag tgagaagggt
     1441 tgttttgatg aataagattt cttgttaaca agaggttcat ctggcaatgt tggttctatt
     1501 gattcggtag ctgattcaat agaaggaaaa tcaatcggca gacctaaagt atgatgtaaa
     1561 agtgacagca tttcaaagtc tggcattgta cgagaatttt cccaactaga aatcgtttta
     1621 tcggaaacac caattttttc gcctaattct ttttgcgtaa gcttacaatt tctgcggtaa
     1681 gttcgtattt tttctccggt gttcatcttc ttctcctttc gattctacag tatatagatt
     1741 tattctacat gacctataca cttgatagat acgttttttt gatgaaaaac ggtatgattt
     1801 tatcaaagaa tcatttaagt aagggagagt ggtgataatc aaaaaaaagt tcacagtttt
     1861 actcacaatc atagtgaccg gtatcatgag tggttttgcc ttggttagta taaataaaca
     1921 ggtactctat cggacaaatg aatttttcca tcaaacagat acttatactc cgattcgatt
     1981 gccacaacat tttattgggc aagaagaata tgagcagcta atcatggtat ttgaaaatgt
     2041 ctcatcagaa actgagcttt cttatatgaa aaaaacagta aatgaatcca atgaagtaga
     2101 tacatcttgg aacttcaaga tacagccaaa gcttgaaatg acattgtatt atcaagggga
     2161 tgtcaaatct tctcacgaga aggtacccta ttctaatacc tcctttcgcg taacgtccat
     2221 tgaagaatcg atcaaaataa aagattttga aggtgaattt ttcttgatga cgagagaaaa
     2281 agaagcctat gaacgggtca tttcactatt cactgatcga ttcaatcaag catttcacac
     2341 tgattatgcc actaatattt ttgaagactt tgaaaatagc tattcgtatt cggttatttc
     2401 aaatacagac gtatataatt atcgagtgta tttaactatt ggattgattc ttttattcct
     2461 tttgttatca tcctttttct tattgatgaa taaagaaatc agtttattgt tgaataatga
     2521 cttttcatta catcaagtat tgtggtgttt attggggaaa aagaaaatca ttattacatt
     2581 cataattgca ggaatcattg gtggttttat ttttatgtat tataaggagc aatttcttta
     2641 tttgttccag atgtacgttt tcttgttcag cggaatatat ggtatctctt tgatttctct
     2701 tttattagtt gaaaaaatcg cttcgttaag agaagcgttc aactatatcc aatacattct
     2761 ttttacattt ggattgttgt tttctcttct tttgaccgtt gccaatgtcg aattagcaac
     2821 tatcgttttt tcttcttcac agttagctgt ttcccatgaa ccaccaaaag aaatcgctgg
     2881 tgaggactat catgtatttt atccagtaac catcgggaaa aaccacgtgg attttattta
     2941 ttccaatcgt tttgcatcag cagcagatca ggaattatat gaaactttga ataaaaacgg
     3001 aagtatatta gtcaatgtat cagattactt gaatgaagaa aatgaacagt ttggcagagg
     3061 gataaaaata aatctgaatt atcttaagaa atttcctttg attgatgtct ctggaaggtt
     3121 gatccaaata accgaaaaag aaacgcaccc tgttatattg atccctgaac gatatagaca
     3181 tacagattta aaaaatgacc tggcgcaaat aacagaatat tatcaagaaa tcagtgaaaa
     3241 agaaccgaaa tttctattta taaaaaacaa tcaacccatt tatacattta ttccttctat
     3301 cccctggatc gaacattatc cggttgttga aatactgaca ttgaaaaaca gtatgtattt
     3361 ggaaagaaat attctcagcg gagaaatcta tccaccgtta aagatcaaga taggctcttt
     3421 gtcgaaagaa aggttatttg agctgattga agaaagtcag ttacgtgata acttgcagtt
     3481 atcatttccg tatactcaga ctgaagagat agctgtcaaa gtgttaagca gatcatttca
     3541 ttacttgata cagatatttt tactcacttt cttttctttc ttcttgttga gctatgtcat
     3601 gttatgcata tattttgtct acaattgtcg gaaaatcgct atctttcgtt caagtggtta
     3661 ttcgctcttt gaaacgtata aagatttctt catgatgaac ctcatcaaat ggggaacgac
     3721 aagtgtaatc ttcctttttc ttatagaaag agagcctaaa tatcttttta atattttttt
     3781 ctttagcttc attgatttta ttctttcagt tgtttttatt ttgtctacag aaaagaaaag
     3841 tcagttattg ctgatgaatg gaggttaaca attggagccg ttgatagtga taaaaggtgc
     3901 gaaaaaaatc attcgaaaca aaacgattct tcatttggat cgtttcactg cggatgctgg
     3961 ggatcttgtg actgttgttg gagaaagtgg agcaggtaaa agtacgttat tgaatatatt
     4021 aggattgaat gatcagttca gttcaggaca attctatttg ttcgatcaag agacgaaaca
     4081 attaactggg agagagaagc gcttgataaa aagaaatacc atatcttttc tttttcaaga
     4141 ttttgggttg attgaatatg aaacaatcga ttttaattta gatattggat tgaagttttc
     4201 gaatttaaat gtttcagata agcgaacagc aaagtatgag gcattgaaaa aagtaaatat
     4261 aaacaagaag ctaagtacgc ctatctacac attatctggt ggtgagaagc aaagaatcgc
     4321 ctgtgcgaga gtcttattga agaatagcaa aatcatttta gccgatgaac cgactgggtc
     4381 attggataaa agaaataagg ataaagtagt tgaaatctta tcgcaacaag cttcccaagg
     4441 tacgttagtt atcgtagtca cgcatgatcc atatctgacc aaaataggaa acaagaagat
     4501 catgctttaa aaatcaaaaa ggaaaggatg tcgttttatg aaaaaagcag ttattatttg
     4561 tgcactaggg ataggttttg ttggaggaaa cgctatgtca tctgaagtgg aagcaaattc
     4621 agttcacggt gaaatctatt acaaatcttt aaagccagga gagattcatt ttgatgctaa
     4681 aggtaatatt attaagtcaa atttaccagt agaccgggaa atttctagaa cagcaattaa
     4741 tcatagctct ggaagatggg tttattatag tgatacaact tgtacgagaa aaacaggtca
     4801 ttctaaccac tatagtcgtg tctataatcg tcacggtgca aaagcgaaag ttggaaactc
     4861 tattcgatca gcaaatgcaa caaaaggagt atgggcctat gcaagtgcga caggaaatag
     4921 aactgacaca ttctcatgtt ggtacaatcc aaccggttgg tattaagtat ccaaaagttt
     4981 atataaaata gaaaaaacat ataatgaaac agcgttaaaa gttatttttg gtagttttaa
     5041 tatattcatt tttagttgta taaaaatata tcataataaa aacaacaaaa aaatgtctgg
     5101 aaaatcgaac ataaaagtgt ctgatttgaa tatcccagca gcggaagaag aaaaacaaaa
     5161 tcgataacat ttgttctaag aattagttac gttaaatacg gaacgcaaga tagacttgaa
     5221 aaacggtaac aaaagaagta gtatattaag gtgtgatgtg ctacttgaca tttattctgc
     5281 tgagcgaact ttctggattg tgtagcctta tatctattca ggacgtatcg agcagattag
     5341 taaaggtgtt cttgatgatt ttcatcttga acacctttgt ttttagcaat tcgcaaaaaa
     5401 cgaaaagtag gagaattgat aaatgaaaaa aagattttta gcacttgcca tcgtactagg
     5461 cactggctta ttgtcaggct gtacgaatgc cggtgaaaaa acagccgtta gttataaagg
     5521 cggtacaatc agcgaacaag aagtaatgga tagcttgaaa aaaatgcaag gagcagattc
     5581 cgctgtgcaa cagttgatcg tttatcaagt atttgaagat aaatatggag atgacgtatc
     5641 aaccaaagaa atcgattctc aatatgatca aacaaagaaa caattgggtg attcatttga
     5701 ttcacaattg aaatctgcag gttacacaga acaaacattc aaagacagta tcaaacaaag
     5761 tctagccttt caagaaggtc taaagaaaca tatcaaattg accgatgaag atttaaaaac
     5821 ggcttgggaa tctttccatc cagaagtgga agctcaaatt atccaagtag caagtgaaga
     5881 tgatgcaaaa gatgtgaaga aagctgctga taagggagac gatttctcta aattagccaa
     5941 agacaagtca actgatacta cgacaaaaga agatggcgga aaagtgaaat ttgattccac
     6001 tactacaacg gttccggcag aagtcaaaga agcagccttt aagttgaaag atggtcaagt
     6061 ttctgatgtg atcacttcta ccaatgcttc aacttatact acggaatatt atgtggttaa
     6121 aatggtgaaa aaccaaaaca aaggcaacga catggataaa tacaaaaaag aattgaaaga
     6181 aattgcaaca gacacaaaat taagtgacag taccttccag aataaagtaa ttggcgaagt
     6241 attgaaagac gctaatgtaa aaatcaaaga caaagacttt gaaaatgttt tgagtacatt
     6301 cacttcagat tctagtacag caagtagtaa ataagcggta taaataaaaa atatgaacta
     6361 tttaaatcaa taaaactgtt catctaaatc tttttattag aactaaatca acagtagttt
     6421 aattactact tgatttagtt ctttttattt taaaaatttt ttttacttag aatccagcga
     6481 ccctagatat ttttctgtat aatcttaaat aaagaaaaca atatcaaaaa atgcacgtag
     6541 aagtagataa atggagtggt tttgcaaatt tcttttattt ctagaagtta ttcacgtata
     6601 cggatttgtt tttctcacaa ataaaaaggc aggaagtaca atggatcatt tgtatataaa
     6661 atatattcat caattttccg ttttggtgtt aggcacacta attatagcgg ggattgtcgc
     6721 atttattatt caaggccgtg acatattaac agtacatcaa gagtttttca gttatgcatt
     6781 tggtattcct ctgatcgcta taaggtctga taacaaaaag tggaagaaac gctattacat
     6841 tctcttgcta tttttgtgtt tttatctgcc acctaagatt tttactttat taggtataca
     6901 atgcctatca ttttctttta tttcctatat cattgtttta attttcctca gtttcatagt
     6961 atggatgttt tatcttgtca aaaaataaat gaatgtttca tgaacagtac ttcatcacag
     7021 cctaattttt ttctttatgc tatcgttata ttaaagaaat caatcagaag ggaaagaaag
     7081 tgatgaaaaa agttttatta gtggtaaacc ccagttcagg cggtgaacaa gcaaaggaat
     7141 ttgagcaatt agcgatagca aaattggaat cggtgtttga tgaagtggtc gtcttgcata
     7201 caaaaaaagc aggggatgca aaaaacttta ctcgcgaagc ggctacggaa gggtatcata
     7261 gtgtgtttgt catgggcgga gatggaacag tcaacgaagg aatcagtgga atcgctgaac
     7321 aagaacatcg gccaaatttc ggatttttcc cattgggtac ggtgaatgac ttggctcgag
     7381 cattagggat accgctagaa cctgaggaag cgatcaatca tttttcgatt gaatcagtca
     7441 agccattgga tattggaaaa ataaatgacg actattttat gaatgtcgtc gcaattggct
     7501 ctatccctga atcaatcaat gatgtagatg cggagaagaa gacgaaattt ggaaaactag
     7561 cttatttcat gtcaggaatc aaacagttag cttccacaca atcctactcg tttcacgtag
     7621 aagttgatgg aaagaaggaa gaaattgaga gcagtacctt gctgatagga ctaactaact
     7681 cagtcggcgg ttttgaaaca ttacttccaa atgcaaaagt ggatgatggt aagctccatt
     7741 tggtttattt aaaagattct tctttgatcg atacgcttaa aacattgcca gacctattaa
     7801 aaggagtaga tgaatctacg aaaaatctag cctaccttac ttgtgagcaa ttagttgtgt
     7861 cattagctga tgaggaagaa ttgacgacga atgttgatgg agatgaaggg gataaattac
     7921 ctgtgacaat caaagtattg ccctcacatt tacaggttta ttgttaaaat ggaggttgtg
     7981 accgaggagt tggcgggaga acaccgcttg ttcgggataa gggactgtga caagactttt
     8041 gtcacagcct tttttttgta caaaaaagaa atttacacct aatagaaaaa tttttccata
     8101 aaaatacata ttttaaatat gaaagtacaa ttggaaatag attaaaacga taaaataaaa
     8161 ttgtcaaaac gtcagagagg atggtggaga atgaatcaat gtgtaaaaaa gctgattgcg
     8221 tcgattttgt ttgtcttccc tctcttgatg gtgacatccg tttttgccga tgtagagaaa
     8281 acaaacgaat caacgcaatc agagaatcaa agttttgaca agactacttt ttcagaagaa
     8341 tcgattactt ctgaaactac agaatctcaa agtgaggctt ctactaataa aaggcaagaa
     8401 gaaaataaga caagtgagac ctcagaagta gctgaacaaa cgtctgatag cagtgaagcg
     8461 gcactggcaa gagctgtttc agcggatggg tacagcgaag ctgccacaac agaagaactg
     8521 gcgcagcttc tcgcagacga atcagtaact aagatccgac tgattcagcc tttgacttta
     8581 gacagggaat tagagatcaa acgagatatc gttattgatt ttggtggatt cgctcacaat
     8641 ttcggcacac accatattta tatcaatgag atacctcagt cgatcgagtt ccagaatttt
     8701 aaaggaacag ctgcacatcc aggtggattg acaccaaata tcgatggaaa tgccattatc
     8761 attgcttata tgaccgattt ttggggaagc agatatcatt ttacgggaga aataaaattt
     8821 accgggacac ttgatttgta tgatgggtcc aaattaggac tgatttacgc tcctcgtgca
     8881 actgtcacat tagatggtgt ttccggtgtg ttggatgtcc aaccagtgaa ggaaggactg
     8941 aatgccgctc cgggtaaagc ctatttttgt agaagctatc aattgaatgt gatcaatggt
     9001 agtcaacttt atggaccgta tctagggaaa ttctacggtt ttctagattc aggagatgag
     9061 actggtagtg gaaaaacagc gccaggaatc catatcttaa gcggttcaaa ggtctctctt
     9121 gattatgatc gggcagatgg cgttagcgat gaaggagagg cagtcgatac gctacctggt
     9181 aatgtcatgt ttgaagtcag tggttcggga agcgaatttt cggtaaatac aaagatcaat
     9241 attaccaatg acaacaatcg cggaatcatc caaatgagag gttcaggatc gaaagtcact
     9301 gtatcgaatg gtgggaaaat caccatcaat acagcaacta cgggcggatt tcgcttgcaa
     9361 ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa aatggctgga
     9421 gatgacgatc aacccgggaa caatgggatg cgttttgtag gaaaaggcct ttctttgatc
     9481 gtagaaggag ccggatcagt tatcgacatc gataagcaga gtgggcgttc cagcgcagtt
     9541 cgttttgaga atggtacgca aaagctgaca gtaaccaatg gaggctcatt gagggtaaga
     9601 aatacaggtg atggcaaatc ttacgtgaac agactcaatc aaggaaatca agcgatccaa
     9661 ttttatgacg cttctggttt tctagaaagt ccaggttcag ctgattttac agtcactgga
     9721 gaaaaatcaa atatcgatat acgcgcagat tccggagcaa ctgtagatac aactggagat
     9781 attgatttaa attttacggc aggtgaaaaa acttatttag tcatggtcgg gaaaacaggg
     9841 gaagataaga ccggcatcat cagtggggat gtgttgaatg tggacttaga atctcctacc
     9901 tattttgatt tccaaaacaa acggattgga agcgagacca atggcatagg cggctgggta
     9961 ttccaaggaa atggagattc ttctttaaag atcgcccatt cagaaatcgc tttatggcga
    10021 aaaacaggcg caacattcga taactttgaa aaagacccgg attatttttc tgatatgaca
    10081 gacttatcga tcaatgggac cgacttaggc aatttcgtat caagccctag tgcaggttta
    10141 acaaatgaat ttgctcagac aggtgccgga atgaaaaatt ataatcgaat cagtgccaat
    10201 aatcagctcc cgatgattga atccttaaga gtgccaacga atgcagataa aaaaatctat
    10261 gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg gacaggcgaa
    10321 gtagaagtga ctttacgtat tacttacgca gatacgacga aatcatctgt agaaattact
    10381 gctctcacgc aaggacaaac agatgaaaat gaaggggcag gatataaccc atggggagaa
    10441 ggcgaacaag gcggcttgtt cgaagttcaa gtacccaatg atgagagatt aaaaacggag
    10501 gatcaagtgc aagttgttgc tgctaaaaaa gtaagaggtg gccaagaagt gacagaactg
    10561 gactctgcca aaacgacagt cgatgtgatt cctcctgaac ctgctaattt aagtaccaca
    10621 ttgatcaatg cagcttctcg cacgatcaca ggtacaggtg aagcaggagc cttggtttca
    10681 ttgaaaaaaa atcaagctgt tttgtcagaa acaactattg atgatacagg ggaatttgtt
    10741 ctttctatcc cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac
    10801 caagcaggag aagctactgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt
    10861 gggaatcaca atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct
    10921 gctacgaaga tcgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgatttt
    10981 ggtcagatca aagcaactgg tttgacacaa gaagcatttg gccaaatgga tatacaggaa
    11041 aaattatgga tttatgatca gcgagaaacg aaaaatccgt ggatcttaac gataaaatta
    11101 tctcagcctt tcacaaatga tgcaggattt tctatgcagg atgtcctata tttcaagaaa
    11161 ggagaggagt atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca
    11221 acgaatgtgc aagcagatta ttcagaatat ttaaataacg gtcgggcgtt taaacttgtc
    11281 ttaaccaaag aaaatcagat agctggtaat tactcagcag agataacatg gacattagca
    11341 gatgcgccga cagaataaag atcaaggagg atgattgatg aatcgaatat ggaataaaag
    11401 tcatcgaaac aactgctctt tacttatgat ccttctcctt ggattcatcc tttggcagac
    11461 acctgtccat gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat
    11521 tgataagaac aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc
    11581 ctacacggtc tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc
    11641 tacgacgaat tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac
    11701 attaagttat gatatgaaaa acttagcaaa aacagtggga gaaataactg ttcctcctca
    11761 aagtgcggtc actgagacac tttcactgaa agcgccagaa aaagagctgg ctggtgtggt
    11821 cgcaggaggg atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg
    11881 tgtcgtcaat cagttttctc aagtgaaagc aatcttgatt tgaaatagcg aaaaagagtc
    11941 ttccccagat ttgaaattaa aaaaagctta tgcggatcaa agcgatggaa gaaatgtgat
    12001 taaggcgaat cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga
    12061 aatctacaaa aaagaaacaa aagagcctta cctttcaata aaaaaagaaa aaatagatgt
    12121 agcacccaat acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg
    12181 acattatcaa gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga
    12241 ttttgaaata actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa
    12301 tcagccagaa acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt
    12361 atttgctgga ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca
    12421 ttaagtaccg ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat
    12481 aagagctcag ccactacaga aggaacgatt tctgtgacca aaccagatgc tgcaaatcct
    12541 gaagacgttg ttccagttcc tcctgttgat ccaaatgatc ctgatactcc ttttacacct
    12601 gacgaaccaa atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat
    12661 tttggaacaa tcaagttagg tacagcaaaa acagtatatg ctgcattaca aagcggaacg
    12721 gacagtactg gagcacaaac acaagtacag aatttcgtac aagtaacgga taaaagcgga
    12781 aattatgccg gttggaaatt atcagtcaaa cgaacagaat ttattggtac aacatctgat
    12841 acgaataaac taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca
    12901 actaatagca gtggaatgcc atctaccgtt tctggaactg gcgctgaagg aatcgaaatc
    12961 ccagcagatc aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg
    13021 atttattcat taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct
    13081 gtatccaatt atgctgtaga caactacgtt tcaacattca catggtcgtt aacagatgct
    13141 cctacagaag aggaagcgta aaatcatatc aacgacttca gggagtggag ccgattcaag
    13201 gcccactccc tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag
    13261 ttttttggaa gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta
    13321 aggagtcacc gttgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg
    13381 ataagttgat acagtaagat tttaaaagta aaaagtaagc atacttttct aactctgtct
    13441 tcaattgttt taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt
    13501 ggtttttcaa acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac
    13561 ttccaccttt ttgatcaata aagattttat cgacatttac gatctgctgt tctttccaat
    13621 tgatctgttc tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc
    13681 caatcggaag gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa
    13741 aaacaatatt actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga
    13801 gtgattgctt caagtcgata tcgataaaga aaacatcgtg attatcaata gtgcccaact
    13861 ggatcaactg attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat
    13921 tgaccaattc tctgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact
    13981 ttttcatatg caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag
    14041 gaataagatg gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg
    14101 tatctattta ttcttttcga aacaactgct agtaaaaaac agctttggtt gtatagcttt
    14161 gatggtggtg acacttttac ttcctatccc taaagtgagc tttccttacc taattggctt
    14221 attattgatg tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag
    14281 taatcttttt tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta
    14341 tttaggaaag agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt
    14401 ctatctatta gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta
    14461 tccaactgaa cactattttg gaggtatttg tattttatta agctggggcg tgatttactt
    14521 ggtattggga agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat
    14581 agtcatcagt tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga
    14641 aagacaacat caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct
    14701 agaggaattc aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga
    14761 aaataaaaaa gaaacgttgg aatttttaga aatattagaa gatcattctg cagctatttt
    14821 tgaaaaaccg caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt
    14881 tttaaaattt ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt
    14941 cgaagtgaaa acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat
    15001 ttctaacgca ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga
    15061 agaaaaagag ggatcacgtt ttagtatagc gaacaaggca aatccaacga atttgacgct
    15121 atcggagatg gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag
    15181 tgcgaaaaaa atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca
    15241 gaatcactat ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg
    15301 acttactgtc aaaggataag tttgaggaaa gtctacacgg agatagagac aacaacacag
    15361 catattcaaa agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg
    15421 tagttaaaca aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat
    15481 gatactaagt agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt
    15541 tgcttacacc agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagca
    15601 aatccctacg atcacaaaaa aaatgctgac ccagcagtta agagagttag aacaagatga
    15661 aagtatccat cgaaaagcct ataaccaagt cccgcctaaa gtagagtact cgctcaccga
    15721 agaaggaaaa tcattaagag agattctagt agcgttgtct gtttgggaag aagaacgtat
    15781 cgaaaaacaa aaagcctcaa gggaagaaga cacgctactg catacacatg gtggctacct
    15841 aaattattga ttttctatga catttatttt aaataaaaga aaacaggagt ttttttaaaa
    15901 gcatcatcta ttttttatgt aaaaaaatgg tatgattaat caagtaagaa gtgattggaa
    15961 gaaggcaagc aaatgaatag tatggaaaaa gtcaaccagt ttagagatga acgtaattgg
    16021 cgacaatttc ataacgaaaa agatttggct atctctattt ctttggaagc atctgaattg
    16081 ttagaacttt ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt agaacgaatc
    16141 aaagaagaac tggcagatgt attgatctat tcatacatga tggcagacaa ccttcacctg
    16201 gatctagatg agatcattga agaaaaattg atcaaaaata atttgaaata tcctgtagaa
    16261 aaaagcgcag gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat tcggctgttg
    16321 gaaagagcaa aagaaaaaca gtaaaatgag tctggaggga gagaaaatga aaaaaaaagg
    16381 aaaagcaaaa agatggctag tcaatatcct cttgttcctc ttgttattag taggactagc
    16441 tttgattttc aatgaacaga tcaaggatta ttttgtcaga gagacaggag ataaatatgc
    16501 tatagcgaat gtaacaaaag aagatctgaa aaagaataac gataaagatg tcagttttga
    16561 ctttgatgcg gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa tgagaggaac
    16621 agatctacca gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt taccggtttt
    16681 caaaggcttg gataatacgt cactacttta cggagcagga acattatctc cagatcaaga
    16741 aatgggaaaa ggaaattatg ccttagcaag ccatcgtgca acgaaccctg aattgctgtt
    16801 tacaccgcta gaaaatttag agatgggtgc caagatctat ttgacagatt tagaaaatgt
    16861 gtatacatac aaaacatttt ttaaagaaaa agttgctcct acagataccc aattactgaa
    16921 tgaagtcgag ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg acgccgtcac
    16981 gcgactagtt gtacaaggag aactggaaag tgttacatcg ataaaagatg cgacagatga
    17041 tatgagatct gctttcaatc tagagacaaa aacattttag gatactggga cataagtcgt
    17101 tacagattaa aaacagccaa aggatatgaa tcgataagga aaaaccaatt tgctgaaaaa
    17161 aagagaaacg caaagaggtg catctctgat ttggttgttt tctagataga cttgtgttcc
    17221 agttttttta ttttttatat gaaaaggctt gcataaataa aaattcgatg gtatgatgat
    17281 tttgaataag aaatggattg ttactgtacg gcaggcaaaa ccagaattca gctaagaaac
    17341 agtaggcgaa ttctaggttt ttttattttt cttgagaggg cgaagcaaaa tgaaaattga
    17401 taaaattttg aataacaatg tcgtcatttc gaaaaatgga ttcggggaag aagtcgtttg
    17461 tatgggaaaa ggactagctt ttcaaaaaaa gattggggac gagatttcgc cagaagcggt
    17521 ccaaaaagaa tttgttttaa gagattcttt tgctaaaaac caattccagc agctgatggc
    17581 tgatattccg gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa
    17641 actaaaagtt gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc
    17701 gatcacacga gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa
    17761 attttatccg aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac
    17821 gggtgtggaa ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca
    17881 acaaggaaac gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact
    17941 gagtatcatc agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg
    18001 tatcattact catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga
    18061 gcaagatgaa tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt
    18121 cgaacggatc aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca
    18181 gatttattta acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata
    18241 aggattgtta ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg
    18301 tgactgtttt tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac
    18361 aatggataat caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt
    18421 caaaagttta gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc
    18481 agatacgcaa aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca
    18541 gtatcaagta gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga
    18601 aggtcttgca gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat
    18661 cgatattatc tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt
    18721 gaaaggattc ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta
    18781 tcaaatattg tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt
    18841 tacagcagct aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact
    18901 tgtgtatcca gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat
    18961 cccagtgatt ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt
    19021 atgggttcaa agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat
    19081 gattttagtt ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg
    19141 accaatcggg acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt
    19201 tagtccaatc gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg
    19261 catgcattgg ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt
    19321 attgatgcca atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc
    19381 attgcgaaca aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt
    19441 atttggaatc actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat
    19501 tgccgcttgt atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc
    19561 tttctccagc agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg
    19621 tgtagagtca aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt
    19681 cgtaggtaca ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa
    19741 acatgcgaat gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac
    19801 gggtaaagta ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg
    19861 aaaaggtatc gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat
    19921 cacgactatt tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat
    19981 tttgattcat atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt
    20041 caaacaaggg gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat
    20101 tagagcagct ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc
    20161 cgatatactt gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt
    20221 aaaataagga ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag
    20281 caactgctgc caatcaatta gaaggggctt atgatcgaga cggaaaaggg ctatccgttg
    20341 cagatgctat gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact
    20401 ggacgattga tcaagaaaaa tatatttacc cgaatcatcg aggaattgat cattacgatc
    20461 gtttcaaaga agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga
    20521 ttgcctggac acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag
    20581 aattttatga tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga
    20641 tttctcatta cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa
    20701 aactgatcga cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag
    20761 tcaaatattg gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc
    20821 aaggattagt aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata
    20881 atcaattcgt tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc
    20941 aaatcggctg catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc
    21001 aggcagcaac aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac
    21061 gtggagaata ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa
    21121 aattagagca aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg
    21181 gatttagtta ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag
    21241 aagggaactt gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc
    21301 agatcgatcc agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac
    21361 cattattcat tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga
    21421 ttattgatga ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag
    21481 tagcagacgg tgtagatttg atgggctata cgccttgggg atgtatggat cttgtcagtg
    21541 catcaacagg agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag
    21601 gaaatgggac actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg
    21661 cctcaaatgg cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca
    21721 tgtgatttgt gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa
    21781 gggtgacgtt atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga
    21841 agatattttc tcaggaaaat atccagtcgg ttcgatgtta ccgacagaaa gtgaattaga
    21901 agaactgttt caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga
    21961 tgaatatgtc gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa
    22021 tcgattatcc aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg
    22081 gaaagaaacg ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt
    22141 tttcggtgag caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat
    22201 ttattttgtt cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa
    22261 agaatctttg tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga
    22321 tttctcggca gtatttttaa cagaagaaga acaagaaatt ctgaaaacag acgcacagat
    22381 cggcatcaaa cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga
    22441 ggcggtctac catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa
    22501 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt
    22561 ctcatattag cttctcatat ttttcgtttg gttaggctta ttaccgcaga tacccggctg
    22621 tttctggatt accatttatt ctggataagg gggtgtggca cgattttcag aaggaatcta
    22681 tgaaccaatc actgtattga ttttagcttc gatggtaacc ccattcggct tgacgatcgc
    22741 ttatttctta gggaaaatca ttcgtaaaaa tatattcaat cgtcaagaaa tcgatacatt
    22801 gaagaccgct tttcctatgg ggatctgtca gatcacagag ggatgtttcc ctatcgtatt
    22861 gaacgacttg gcacgcaatg tcatagcaac gggagtcggt ggggcagtcg gcggtggatt
    22921 gagcatgttt tggggcgcag atagccgtat tccggcatcg ggaatgtttg cagtcgctac
    22981 gatgaccaga ccatgggcat tcattggtgc attgttagct ggttcttttg ctacgggaat
    23041 aactatttta ttattgaaaa aaccagttga cccaaatgca gaagtcatcc aagaagaaaa
    23101 agaggaagaa gagatatctt gggatgactt gacaatcagt taggaggaac agaatggatt
    23161 acaagaaaag agtacaacaa atgttcctag attcaggagt agtattgaaa gaaacagaat
    23221 tagaaagaat cgaatataca gattttggat taggagcaat cgagactgaa ggattgaatt
    23281 tatttgttta tgaaaataat gagcgatact gtgggaaaga actggtatta ttgccttatc
    23341 aaacttgtcc agagcatcgg cacccacctc gagatgagaa tgaaggaaaa aaggaaacct
    23401 ttcgttgtcg aaaaggctta gtatatcttt atgtcgaagg aaaagaaaca aaaaaacttt
    23461 cctgtattat tccaaagggg caagaacgtt actatacggc aaaaaaagaa atcatacttc
    23521 acgctggaga tcagtacacg atcgaacaaa atattaaaca ctggttccag gcaggtgcaa
    23581 caggcgcggt tatcaccgaa ttttcttcca gcagtgatga tccaagtgat agttttacga
    23641 atccagctat ttatcgttaa tacaaaggtt tgggaacaag ctgatgaacc aaaatgatca
    23701 aatttcgatt atttggttcg tcggcttttt tatttagaga aacatcaggc gttggtgaaa
    23761 cataacacga gaataaaatg gatttttcat taataggaga aacaagtgtg tgactattta
    23821 aataatgcgt gttattgaca tgcaggacga aatcccaatt tcttctataa gtaagtcgtt
    23881 tcaattttat atttactagt tatttatttt tttggttact gtaaaatagc tgaaaaatta
    23941 tttttttgca aatactatat atagtggtgt ttgatctata taccactaaa aaactacaaa
    24001 atctagtctt ttattttaaa aaaacacgtg tagaatgaag atagactttt gagttaggga
    24061 gtgttttttt atgaaactta ctgttttttg cggttcccgt tttggcaata aagaaagtta
    24121 caaatttatt gcgcaaacgt taggaaaata tatggcacaa gaagaaatcg aactggttta
    24181 cggtggttca tactccggga ttatgggagt gattagtaaa acagtactgg aaaataacgg
    24241 aaaagtcaca ggtatctatc caaatggcct ttttgaaact gagctgcctg ggaaagatgt
    24301 tactacattt attcctacag agacaattga tgaaagaaag gtattattat ttgaaaaagg
    24361 agatgctgtc ctcgttttcc ctggaggtct aggtacattg gaggaatttt cgcaacttct
    24421 ttcttggata gcgatcggcc tgacacctga taagccaatc ggaatattga atattggcgg
    24481 ttattacagc ggcttgcaac aattattaga aacttttgca aaagaggatt ttatggataa
    24541 aaaatggttg gatcaagtga tcttttccaa caatccattg gaactggtca atgaattacg
    24601 agcagtatct attaaaaatc agttattatt gaaggaggca aaataatgag tcaaatatat
    24661 ttagcaggac catttttttc agaggagcaa atcgatcgag taagtcgtat cgaaaaagcg
    24721 ttggaggaaa acaaaacagt cacaagtttc tactctccac gtcatcacca agaaagcaat
    24781 tatgaactat tcagcgctgg atgggcgcag gaagtatacg aaaaagatat ggaagagttg
    24841 acaaatgcag aatttgtttt ggctatcctt gactttgagc atcaaacaat cgatccagga
    24901 acggcttacg aattaggtgt ggcaactatg ttgaaaaaac cgatgatcat cgtacaagaa
    24961 gagacggtac ctacgaactt gatgattaca caaagtcttc acacgtattt aaaatcagac
    25021 caagctgtac gtgaatatga ttttgaaaaa ttgcctgttg aaacttacgt aggcgagtac
    25081 ttatagaaga ggaagggttg tatgtcagtg attttgattt caggaacgat tggtgcaggt
    25141 aagagcagtt tgacggatat gcttgcaaaa gagattgatt ctaaaccatt ttatgaaaat
    25201 gtcgaagata acgaagtatt gccgcttttt tattcaaatc ctgagcagta cgcattttta
    25261 ttgcaaatct tctttttgaa taagcgattt ttagcaatga aaaatgcttt agtgaacgat
    25321 gacaatgtgc tagaccgctc catctacgaa gatagtctgt tgttccatct aaatgctgat
    25381 ttaggtcggg taacggatat cgaagtacag cagtacgata gcttattaga tacgatgcta
    25441 aacgagttag atgatgttgc tccaaaaaaa cgtcctgatt tgatggtcca tatcaaagtt
    25501 tctttggaca cgatgctaga acgtatcaaa aaacgcgggc gtgactatga acagctagaa
    25561 agcgatgaaa cgttatacac ctattacgaa acactgaaca cccgctataa tcaatggtat
    25621 gaggattttg atgtatgtcc taaaatacaa gtggatgggg atacattcga ttttgtagaa
    25681 aatccagaag atgcaaaaaa ggtgattcgt cagatccaag aaaaattagc tgaattggaa
    25741 ggaaagcctg ctggaaaata ttttacagat aatcagcatg cgaaaggtca agaattaggc
    25801 taaaagtaaa aaaccaaacg acgggaaaga aaaattacta cgtgatgtag aataagtctt
    25861 tctatcgttt ggttatttta tattcaaaga tgtgaaataa ataaaacgtt actctttgat
    25921 ttgtgaactg aaatgatcca ctgtttgttt tactgattcc agtttttgaa gcttgatcaa
    25981 aggaagaagc tgagtttgaa gttcataaaa atcagccata gtgagttttg cttgattttg
    26041 cttatcttct tttgcttttt acataatttt tgacaggata agattcaaac gatttaaaag
    26101 aatctttgac tagtttgtgg caattgtcgc atatactcaa acttccttga tcattaaatt
    26161 ctgtatggat atttttgttg ttattacatg tacaataatc aggactatcg atcatccatt
    26221 tcgtctcctt tacagtcatc ttgcttcttt actatagcag aaaagaaggg gagaaacaaa
    26281 agaagatcca acagatggca ctatagactg tagatgttgc catccttatc cttcaaacgg
    26341 taattaccga ttttggtaaa tgtgactgtt gcttcttttt tattttcttt tcctgaaata
    26401 atatattcgt catctttggt agtaatcttg atatctgtca gcactgtttt ttcttcagat
    26461 gattcagaag aatcagctcc ttgtgtctga tacgtgcgtg tccattccat ttttttatct
    26521 gtaaatgtca actgtcccgc ggataaatcc atatctttac gtacttttag cgtatataca
    26581 ctgttggtga tatctgcttt ttccgctttt aagacagaag aatcggttgt ttcgtccgag
    26641 gattggtcag agccgccaaa caaatcacag ccagtcaatg tcaaaagtcc cgcagacaaa
    26701 ataaatagta cgattaattt cttcaaaata cttcccccat ttgataggat aaagaaatta
    26761 taccattgga aagtaaaact tcaaacggat ggaagagaaa aaattcacaa aataccatta
    26821 aatagaataa tttttcaaaa gtcataaaaa atccaccacc tttagaaagc gttttaaaaa
    26881 aatgtgttgc aatctttaaa aaaagtgcta tattaaaaat gaaccggttc aattttagaa
    26941 tctaggagat gaaataatga aagtcgtaga cagactatct gaaaaaatgc taccaatcgt
    27001 atctaagatg agtagccagc gacacttgct ggccattcga gacagtttta tcgttactat
    27061 gccgttagta atggctgctt ctgtcatggt tttgctgaat gctctgattt tttcaaatgc
    27121 aacagtccaa agaatacttg atctaagtcc attggctgaa cttgcttcaa tcgtgaataa
    27181 tggtacgatg agtattttag ctattatcgt gtgttacaat atcggctgga atttggcaac
    27241 ccactatatc caaacaggcg tgattgatga tccatcattt agtcctgttc atgcaggaat
    27301 tctaagtaca gccgtaatgt ttattttgat gtctttatca agtacagtca ctttgatgga
    27361 cggaaataca gcagaagcta caggggtttt cttacaaagt ctgacttctt cgtctggctt
    27421 agcaacggcg atgattgctg cccttgtcag tacagaatta tttatcaaat tagcgaaaat
    27481 gaagagtttt aaaatcagga tgccagaagg agttccaccg gcagtagctg cgtcatttaa
    27541 ttctttagta ccagaaatag ttgtcatttt atttttttca gttgtcgttt ttactttaaa
    27601 ccatacgact ggtttgaatg ttccgcaatt aattgaatta gcgattcaga caccattgaa
    27661 agcttttgtt ttatccgtcc caggcatctt atttttacag tttttctctg atttcttatg
    27721 ggtgtttggt atgcatggtt cgagtatttt ggctcctata cggcaagcac ctttgttaca
    27781 gtctattcag gaaaacatgg atgctttttc agtaggaaaa gaaattccta atattattac
    27841 ggagcccttt accaatgcat ttggattgat cggaggtggc gggtgtattt taccgttagt
    27901 tattgctatc ttgtgggctt ctaaacgtca agaacagcga tcaatcgcta aatttggtct
    27961 gactacttgt ttatttaata ttacggaacc aattatgttt ggattaccag tagtcatgaa
    28021 tccaatatat atgattccat gcgctattat accatctatt aacttggtta ttgcctacgc
    28081 agccacaagt cttggaatca tcagtaaaac agttgctgct gcaccttgga tcactccacc
    28141 ggttatacaa tcttttattg ctacaggagg cgatatacga gcagcagtct taacagtaat
    28201 acttatcatt ctagatgtat ttttattcct gccatttgta ttagccgcta ataaggcaaa
    28261 attggcagaa ggaggatatt aaggtgtacg attacccgta tcaaaaccca aagttaacca
    28321 tccgccaaag agtagaagat ttactttcga ggatgaatat tgctgaaaaa gtaggacagg
    28381 taaaccagca tttgtatggt tgggaatgtt atgaaaaaaa cggagacaag atcgaattaa
    28441 cagaaaagtt aaaagaacat gttaaatggg gaggcggtct gggtgcctta tacggtctgt
    28501 ttcgctctga tccgtggtct aaaacagatt ataagaatgg gattcctgca aaagaaagct
    28561 ggaaggtggc taacctagtc caagaatatg tactacagca ttcgcgatgg aaaattccag
    28621 ttttgcttgt agaagaatgt ccacatggtc atcaaggctt ggatgggatt tcttatccaa
    28681 cgaatatcgg ccgtggaaat actttcaatg tagcattatt agaacaatcg agtcgattga
    28741 tggcaaaaga actggctgct aaaggtgttc atttagcgct ggtgtcgact ttggatctgg
    28801 caaaagatcc tcgttgggga aggacagaag agtgctttgg ggaagatccg attctctcag
    28861 ctcgtttttc tgaagcggtc gttcgagggt tccaaggaga gattatttca gaaaaagttt
    28921 cttttcttga tcagacagta gaggaaatca ataagaagaa ggatcagatc ggtgttgtgt
    28981 taaagcactg tatcgcacaa ggagaagctt taggcggtca taattcaggc acagtaacga
    29041 ttggacgcag agagttctcc gatatttacg gaccattatt gaagagtaca agaaatgcag
    29101 taggtgtcat ggctgcttat aatgacatcg acgggatacc ttgtcatatc aatcgagcgc
    29161 tttttgagca aacattaaga aaagagatcg gttatcaagg aatcgttatg gcagacggtg
    29221 tggctttgga tcgtttatcc gatgttttta cagataaaaa gactgctgca gcatatgcat
    29281 tagcggcagg aatcgatctc agtttgtggg atgagaccta taccaagatt gcagaagcca
    29341 ttgataatca agtcgtagat gaaaagctac tagatcaagc agttcgccga gttttatctg
    29401 tcaaattttt attaggttta tttgaacagc catttgcaaa cgatccaaag gagtattggg
    29461 atcatctcat ggaggaatca gagcacttaa atttagagac agcaaaagaa agtatcactt
    29521 tactaaaaaa tgacggtata ttgccgttgg atcaacaagt aaaaaacatt gctgttatag
    29581 gtccaaatgc tcacgatgtt taccacttgt tgggagacta ctctgcacca caaacagaag
    29641 accgtttgaa aaaaaagata gtacaagaga taaaaacaga gtttccccaa tcaaaggttt
    29701 cttatgcaca aggatgtgaa gtaagaaatc aggaggatca ggaagaaaaa ttattagaag
    29761 cggtgaccct tgctcaaaac tcagatgtga tcattgcagt tctaggtgga tcaagtgcgc
    29821 ggaactttga tatggaattt cttcgtaacg gggcagtttc atcgaaaggg atcaacatgg
    29881 attctggaga aaatgtagat gttgcttctc tttctttagg tggataccaa gaacagctga
    29941 ttgaacaatt gtatcaacta ggcaagccaa tcatcacgat acttgtgcaa ggaagaccct
    30001 acgatttgat gaaaatcgag tcatttacga atgccattgc aacagcatgg tttcctggac
    30061 aggagggtgg aaaagccatt gcgcagatga tttctggaag aaataaccca agcggacgat
    30121 tgagtttgtc ttatcctaga aatagtcggc aactacctgt ttattactat cagcgtgctg
    30181 cttctaaaca agagaattat tatgatttaa gcggaagtcc attttatcct tttggacatg
    30241 gtctaagcta tacaacattt gaatacagtc agatggaagt caaaacggat attgacaaag
    30301 taataatatc aattaaagtt aaaaatacag gaagtgtttc aggtaaaaca agtactcttg
    30361 tctatgtccg attaattgac ggcgttgtca tccaaagaac gaaattattg aaagcttttc
    30421 gtaaagatga attgcggtct caagaggaaa gactgcttgt ttttgaactc acttctgaag
    30481 actttagtta tatggatatt gatgacaagc ggcattatgc aaagaaagcg gtcatcatgg
    30541 tcgaacaaca agaaaaagaa atcaaatgta actggcagga gtgaaaatgt atggcatcga
    30601 tccgtgacgt ggctaagatg gctggtgttt cagtgggaac agtttcccgc tatttaaacg
    30661 gtcagcagct gaaggaaaag aatatggaaa aaatatcaga agcgatttca gcgttggact
    30721 ataaagaaaa catcattgct aaaggattga agaataatcg aagtttctct attggactgt
    30781 tgataaacgg gatatctaac cgatttggtt cggaggttgt ttcagggatc gaaagagtag
    30841 ctgaagaaaa tgggtatagc ttattattaa gcgggtttgc cgatcagcca gaaaaaatcg
    30901 aacaaaaaat cgaatacctg atgaagcatg cgatcgatgg attgatcgtc ttcttatcag
    30961 aagaagagtg gtctggattt gaaatgctga caaaaatgag tatcccagtt gtcgcattga
    31021 attgtccggg tgggccagca ggtattgata caatagtagt gaatgatcga gaaagtgtag
    31081 caaaagtgat tgctcatcat ggcggacaag gacataaaac aataggattt attgctgccg
    31141 ctcaaacaga ttacgtagca agagaacgtt tagctggagc caaagaagcg gtacaaaagg
    31201 atccgtctat ccatttagag atctttacag gagactattc tagaaaaagc ggctattacg
    31261 gggcaaaagc attattagaa aaaggggcaa ctgctatttt tgtcagcaat tacaacatgt
    31321 cgattggtgc catcgaatgt ttcaatcaag aaggtgtcag aatcgggaag gatattgcgt
    31381 tcagtcacta tgattattta gataaaaacg atctgagtat ccttccaaaa atcacgatca
    31441 cgccaccaac agataaaatc ggggaacgag cagcagaaca gctgttgaaa cgaatcaaaa
    31501 aagaagagga agaaaatgaa aaagtgattg tgatggaaaa tatcatacat ggaatcaact
    31561 aaaaaagaaa aagaggtgct ttgatgaaaa gatatcaatt tccaaaaaat ttttggtggg
    31621 gctctgcagc aagtggtcca caaacagaag gaagaatttc aggcgatgga aaaggagaga
    31681 atatttttga ttattggtac aaaaacgaac cgcaaaagtt ctttgatcaa gtaggacctg
    31741 aaaaaacctc gcaagtttat acgaagtatc aagaggatgt acagttgatg aaacagactg
    31801 ggcataattc atttcgaacc tctatccaat ggagccgttt gatacctgat gaatctggaa
    31861 caatcaacga agaagccgtt cgtttctatc atcactattt tgatgagttg attgaaaacg
    31921 gtatcgaacc ctttgttaat ctgtatcatt tcgatatgcc gatgtatctg caagaaaaag
    31981 gcggatggtt gaaccgagaa acggttgaag cctatgaaaa atacgcaaaa agttgttttc
    32041 aattgtttgg ctcgaaagta aaaaaatggt ttacccataa tgaaccaatc gttccagtcg
    32101 aggcaggcta tttgtatcga tggcattatc cggaagaatc agatatgaaa aaggcaatcc
    32161 aagttggtta tcatgaagcg ttggcttcag cattagccat caaagcttac catgaaatgg
    32221 atcaaagcgg tgtgatcgga atcatcttaa atttgacacc gagttatcca agagacgaat
    32281 caaatcccga agatgttaaa gcagcacgaa tagcagatgc gttcttcaat cgttcattcc
    32341 ttgatccagc tgtaaaaggc gagtttccca gtgaattgat tgagagaata aaagaactcg
    32401 atctaatgcc ggagattcat ccagatgatt tgacgatcat taaagaaaac acagtagatc
    32461 ttttgggaat taattattac caaccacgcc gaataaaagc aaaagaaaca ccaatcgata
    32521 ctaaaaacgg tcctatgcct gaggattact tcgacaatta tgaaatgcct ggtcgtaaaa
    32581 tgaatcctta tcgcggatgg gagatctatg aaaaaggaat ctatgatatt ttgatcaatg
    32641 tacgagatca ttatgggaat atcgactgtt ttatttctga aaacggcatg ggtgtagaaa
    32701 atgaagcacg atttatcaag gaagatggta tgatccacga tgattatcgg atcgaatttg
    32761 ttcaatctca tctgaaatat gtccatcaag ccattcaaga aggtgccaat tgtttaggtt
    32821 atcatatgtg gacatgcatg gataactggt cgtggaccaa tgcctataag aatcgttacg
    32881 gttttatttc agtagattta gcacaagatg gaaaaagaac gatcaagaaa tcgggtcatt
    32941 ggttcaaaga tgtatcagac agaaatggat ttgaagctta aagaatagcc aagagagaat
    33001 gtatgaaaat tcccgttctt accgcttcag gaaaaagaag ttcaattatt tacgatcttt
    33061 ttcaactttt ttgtctttct atttcatttt ttgtatataa tagaatagtc aaaattaaat
    33121 aggagtggag gaaggcatga ggaaaaaaag accgtttctt ctcttttggt tattcggttt
    33181 tttcttactg cttattttat ccgcagcatt ttcaggaaaa atgacagcag atcttgctca
    33241 gaaagaaaca gaaaaaatcg tagcaagcaa agaatcagta gataatttca aaaaagaatt
    33301 ctcaacttat tggtgggagg aatataaatt aactgccaat tacgaaaaag gtgaattgga
    33361 catctatttg ccaaaaggaa aagaagaaaa tcaatttatc aatatcgatg acgtacttag
    33421 tgcgatttct ctcagacaat atcaattcaa agacaaacat ttggtcgttt tgcttttgac
    33481 tcatgatggc gacaaggtag cacgtttgaa aaatggaaaa gtggttacag cttattctga
    33541 aaaagtggac aaaaaagtgg tggccgatcg actagcggaa tggaaaaaga ccaacgaaca
    33601 agtcaagaaa acaactgctt cttctcaaaa aaacgagtcg acagaactaa gcccttcaga
    33661 ttctggcagt ggacaaacga taacgggaaa tgaagaaaat caagcctcaa tcattacgaa
    33721 tgagtcatca gaaggacaat atcaaaccta tggacaatga aaaacagaag ttgtgacaga
    33781 agtggtcagc ttcaagaaat aagaagaaat ttacgaaaag tgcttctcaa gtttttgtaa
    33841 aatttcggct tatttccgaa gaagctactt ctggaacacc gtgaatcaga ggttgtgaaa
    33901 tcagcggttt gacct
//
LOCUS       DACEDO010000025        33827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEDO010000025 DACEDO010000000
VERSION     DACEDO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.025"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            308..1969
                     /locus_tag="IWO52_001262"
     CDS             308..1969
                     /locus_tag="IWO52_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0355540.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2039..2386)
                     /locus_tag="IWO52_001263"
     CDS             complement(2039..2386)
                     /locus_tag="IWO52_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0355541.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2470..3411
                     /locus_tag="IWO52_001264"
     CDS             2470..3411
                     /locus_tag="IWO52_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355542.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3412..4455
                     /locus_tag="IWO52_001265"
     CDS             3412..4455
                     /locus_tag="IWO52_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355543.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4461..5531
                     /locus_tag="IWO52_001266"
     CDS             4461..5531
                     /locus_tag="IWO52_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355544.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5521..6474
                     /locus_tag="IWO52_001267"
     CDS             5521..6474
                     /locus_tag="IWO52_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0355545.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6486..7496
                     /locus_tag="IWO52_001268"
     CDS             6486..7496
                     /locus_tag="IWO52_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0355546.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7781..8602)
                     /locus_tag="IWO52_001269"
     CDS             complement(7781..8602)
                     /locus_tag="IWO52_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0355547.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8699..8977)
                     /locus_tag="IWO52_001270"
     CDS             complement(8699..8977)
                     /locus_tag="IWO52_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355548.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9245..11254
                     /gene="metG"
                     /locus_tag="IWO52_001271"
     CDS             9245..11254
                     /gene="metG"
                     /locus_tag="IWO52_001271"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0355549.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11733..12320)
                     /locus_tag="IWO52_001272"
     CDS             complement(11733..12320)
                     /locus_tag="IWO52_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355550.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12495..13817
                     /locus_tag="IWO52_001273"
     CDS             12495..13817
                     /locus_tag="IWO52_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0355551.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13972..14748
                     /locus_tag="IWO52_001274"
     CDS             13972..14748
                     /locus_tag="IWO52_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0355552.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14741..15310
                     /gene="rnmV"
                     /locus_tag="IWO52_001275"
     CDS             14741..15310
                     /gene="rnmV"
                     /locus_tag="IWO52_001275"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0355553.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15303..16187
                     /gene="rsmA"
                     /locus_tag="IWO52_001276"
     CDS             15303..16187
                     /gene="rsmA"
                     /locus_tag="IWO52_001276"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0355554.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16638..17267
                     /locus_tag="IWO52_001277"
     CDS             16638..17267
                     /locus_tag="IWO52_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355555.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFGLIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17391..18500)
                     /gene="ugpC"
                     /locus_tag="IWO52_001278"
     CDS             complement(17391..18500)
                     /gene="ugpC"
                     /locus_tag="IWO52_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0355556.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18709..19131
                     /gene="mgsA"
                     /locus_tag="IWO52_001279"
     CDS             18709..19131
                     /gene="mgsA"
                     /locus_tag="IWO52_001279"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0355557.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19454..19990
                     /locus_tag="IWO52_001280"
     CDS             19454..19990
                     /locus_tag="IWO52_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0355558.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20030..21793)
                     /locus_tag="IWO52_001281"
     CDS             complement(20030..21793)
                     /locus_tag="IWO52_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355559.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21796..23511)
                     /locus_tag="IWO52_001282"
     CDS             complement(21796..23511)
                     /locus_tag="IWO52_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355560.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24290..24514)
                     /locus_tag="IWO52_001283"
     CDS             complement(24290..24514)
                     /locus_tag="IWO52_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355561.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24714..27482
                     /locus_tag="IWO52_001284"
     CDS             24714..27482
                     /locus_tag="IWO52_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0355562.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27563..27982
                     /locus_tag="IWO52_001285"
     CDS             27563..27982
                     /locus_tag="IWO52_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0355563.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27994..28464
                     /locus_tag="IWO52_001286"
     CDS             27994..28464
                     /locus_tag="IWO52_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0355564.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28481..29260
                     /locus_tag="IWO52_001287"
     CDS             28481..29260
                     /locus_tag="IWO52_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0355565.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29247..30068
                     /locus_tag="IWO52_001288"
     CDS             29247..30068
                     /locus_tag="IWO52_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0355566.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30085..31134
                     /locus_tag="IWO52_001289"
     CDS             30085..31134
                     /locus_tag="IWO52_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0355567.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31147..32151
                     /locus_tag="IWO52_001290"
     CDS             31147..32151
                     /locus_tag="IWO52_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0355568.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32784..33803
                     /locus_tag="IWO52_001291"
     CDS             32784..33803
                     /locus_tag="IWO52_001291"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0355569.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11044 a         5994 c         6910 g         9879 t
ORIGIN      
        1 accgtttatt cagataaggg actgtgacaa gacttttgtc acagtttctt tttgtttatt
       61 cttttttatc tttgtatggc aataatagac aatataacga aaattattta atggcttttg
      121 gaagaccatg gaaaaccttg tacaaagcta ttttttctga aaaattcctt gaaacctcac
      181 gaaaaaatca ttattttatg aaaaagacaa aaaaaagtgt tgatttttta ataattattc
      241 attaaactct taaacaactt cagaaaatat taattattct gaaaattcca ataaaggggt
      301 acgtaaaatg aaaaagaaat ttacatttgg tttggttgcg gtatgcggcc ttgtacttgc
      361 tggatgttat ggcggcagtt cagccacaag cagtagttca gcaaaaagcg gttcagcaga
      421 tggaggaggc gtatttaact tagtcgtgcc tcaagaaatg cctacagctg acttatcagt
      481 tgcaacagat acaatcagtt ttacagcatt gaataacgtt tatgaaggaa tttaccgtct
      541 tgatgaagac agcaaaccac agccggcagg agctagcgag ttggcagaag tcagtgaaga
      601 cggattaact tacaaaatca aattacgaga agacgcaaaa tggtcaaatg gtgatcctgt
      661 tacagcagcg gattacgttt atggctggca acgaacagta gacccagcta cagcttctga
      721 atatgcttac ctgtatgcgc cagttgaaaa tgcagaagac attacagctg gaaagaaaga
      781 taaatcagaa ctagggatca aagcagttgg tgactatgaa ttagaaatca aattgacgaa
      841 acaaacacca tatttccaat atctgcttgc tttcccatca tttttcccac aaaaacaatc
      901 cgtagtggaa gaaaatgggg atgcctatgc atctgcaagt gataaagctg tttataatgg
      961 accattcaca ttagaagatt ttgatggacc aggaacagat acagaatgga cttataagaa
     1021 aaatgatcat tactgggaca aagatacagt aaaactttct gaaatcaaag tcagtgtagt
     1081 aaaagaatct tctactgcat tgaacttatt caaagatggc caagcagatg acgtgatctt
     1141 gtctggtgag ttagcacagc aaaacgcaaa cgatccagct tatacttctg ttaaagaagc
     1201 gcggacaagc tatatcgaat tcaatcaacg tgaaaagaat tcaccattca acaatgtgaa
     1261 cctaagaaaa gctatttctt attctatcga tcgtgaagct ttagtaaaac aagtactagg
     1321 tgacggatca gttgtctcta caggattgat cccatcagat atggtaaaaa gtccaacaac
     1381 gaacgaagac tttgcaaaag aagctggaaa attagtttct tatgacaaag ataaagcaaa
     1441 agaatattgg gaaaaagcga aaaaagaatt gggcgttgat tctttagaat ttgatttgat
     1501 ggcttctgat gatgattcat cgaaaaaagt aatcgaatat gttcaaaact caatccaaga
     1561 aaacttggat ggcgtaacag tcaaaccaac acctgttcca ttctctgtac gtcttgaccg
     1621 ctcaacttcg ggtgattttg acgcagtact aggcggatgg gctgctgact atgcagaccc
     1681 aagcagcttt actgatttgt ttgtcacagg taattcatac aaccgtggtc gctggagcaa
     1741 tgcagaatat gacgaagcag ttaaagcagc ttcatcaaca gacgctggcg atgaacaagc
     1801 ccgttgggat gatttccaaa aagcaatcaa gatcatctct gatgatatgg gtgtcgttcc
     1861 ggtgtaccaa aaagcagaag gtcacctagt gaatgaaaaa atcaaaggta tcgttcacca
     1921 tgcggcaggc gcttcatggg attacaaatg gacatacgtt gaagaataat cgttaatacc
     1981 gtgagcatga gaaaaaacga ggctgcggca aatgtcgcaa cctcgttctc atattcactt
     2041 atcctaacaa agaaaaaagc atgaaaatag cagaaagagc aattagtatt ccggcaatga
     2101 tcagccaaaa gctgtacata ccgtagctgg cagacgagag cgaggaaaat tctggttttt
     2161 tctttcggtt ccttgttcgt gcaagatgga ccgatcgctg aaaatgatcg aagaaacccg
     2221 aagaaaagac ccataaaaaa ccgccgataa tcagaaacgg caatgaccat aagaatagat
     2281 cattagataa ttgaatgagt gtaaaggaat ggttgatgag attttttatt aaaataacca
     2341 gaatcagtag aaaagctact gcgtagggga tagatttttt tttcatttga ttgctccttt
     2401 atttttggtt cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg
     2461 gaaacattag tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc
     2521 acgctttggt taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca
     2581 tatacgaatc aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt
     2641 ttggataaac cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat
     2701 tttggaattt ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc
     2761 ggaccatcct tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt
     2821 ttaggaacga tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta
     2881 gcgatattag gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt
     2941 gcaatcaaat tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta
     3001 ccaacaattg cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa
     3061 atggtggaag tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt
     3121 tgggaaatcg cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta
     3181 ggaccgttag ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt
     3241 cctggtatcg gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg
     3301 gctgtaacga tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg
     3361 tacggtctag ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg
     3421 ataccaaagc gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa
     3481 aaaaataccg aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat
     3541 tcatggcgcc gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc
     3601 atcattttga tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac
     3661 gtggcttata tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga
     3721 aaagcaatgg ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac
     3781 gtaaatttct ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg
     3841 ggaatgagaa tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt
     3901 gtagcttacg ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt
     3961 tttttggaaa tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt
     4021 tttgaaccag gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg
     4081 gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga
     4141 ttgacattag gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct
     4201 agtgtcatta ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt
     4261 ttaagcttta tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc
     4321 gaagggtaca agacattcct gtatctgcct tatctattat ggattccggc agtaacttta
     4381 tcggttatca tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct
     4441 aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt
     4501 ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag
     4561 gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca
     4621 tcatgcgctt gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta
     4681 acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga
     4741 tgattttcca agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg
     4801 ctgagtccct tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag
     4861 aattgcttaa attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc
     4921 agttctcagg aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg
     4981 aaatcctgat tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt
     5041 tggagctgct gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg
     5101 accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg
     5161 ttgaagttgg aacatcggaa gaaattttct acaacccgca acatccctat acttggggac
     5221 tgcttggttc aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt
     5281 ctccaccgga tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg
     5341 cgatgaaaat cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg
     5401 cagcaacttg gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac
     5461 gtcgctgggc tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg
     5521 atgccaaata aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag
     5581 aatgaagtac atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt
     5641 ttagttggag aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat
     5701 gaacctactg atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa
     5761 aaaggtttga cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg
     5821 ttgaatcctc gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta
     5881 gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg
     5941 aatccaagcc acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt
     6001 ggtattgcac gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct
     6061 gcactagatg tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa
     6121 catcaattga cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac
     6181 cgaatcggtg taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat
     6241 aatcacggtg ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca
     6301 gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta
     6361 cgtcaacttc gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca
     6421 tattacgcaa aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga
     6481 catagatgct aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat
     6541 tgattcgtac ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga
     6601 aaagttggca acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag
     6661 aatacccatt cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg
     6721 atcgggcttt tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa
     6781 aaagtatgtg gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg
     6841 gctacttaaa gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg
     6901 aaggatcgat tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac
     6961 tactttgtga tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct
     7021 atcatccgaa gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc
     7081 accagacaga agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg
     7141 ctcaccatag aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag
     7201 taatggatct ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg
     7261 ggaaattggt caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac
     7321 atgctgattt ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc
     7381 aatggtataa taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg
     7441 taagaaagag ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa
     7501 tgaatgaaac tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt
     7561 tgtttctagg aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca
     7621 atagcagaaa aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt
     7681 tacgaataca tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt
     7741 ttttcttgaa tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct
     7801 tgagctgata gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga
     7861 aatattggag catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta
     7921 agaatttagc aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta
     7981 gaacttgcgg ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt
     8041 gactatggtc aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg
     8101 agatagccac ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc
     8161 agcgtacttc cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt
     8221 agctgactgt gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt
     8281 gctcaatatt ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc
     8341 tttctccagt tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat
     8401 aaggatgccc ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc
     8461 acatcagtac gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt
     8521 gataccagtt aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt
     8581 ctttagaagc aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct
     8641 tattgtagca gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt
     8701 aaatatgaaa aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca
     8761 atactttata ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa
     8821 tgataaaacg caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa
     8881 aataaccata catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag
     8941 caatactaag tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag
     9001 atagatagtc tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga
     9061 tatgaaacaa aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt
     9121 gaacgtagat agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg
     9181 ctttttttat ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag
     9241 ccccgtggca gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa
     9301 acttcatatc ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg
     9361 gttgatgggc tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga
     9421 aacaaaagca gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga
     9481 tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga
     9541 tgattaccat aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat
     9601 ttacttaggt gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac
     9661 acaacttgca gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag
     9721 tggacacgaa gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc
     9781 agaccgtcta ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa
     9841 aaacgaaatg atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac
     9901 aacttttact tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat
     9961 cgatgcatta tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt
    10021 cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac
    10081 gatctattgg ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg
    10141 gcatggctgg ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta
    10201 tcctgaaatg cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc
    10261 tgttccgttt gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta
    10321 tgatctggca aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata
    10381 ttgtgatgga ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc
    10441 tacgacggct gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa
    10501 tacagcattg gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac
    10561 agaaccatgg gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat
    10621 ccatttagct gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac
    10681 gccagagaag atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt
    10741 acactttggc gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc
    10801 acgtttagat atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca
    10861 aacaaacgaa gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga
    10921 aaaacaaatc aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa
    10981 ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag
    11041 ccaagaccgt caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat
    11101 cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag
    11161 tcaaggaatg atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc
    11221 aaaatccatg ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt
    11281 tgtgtcatac cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta
    11341 cttgaagcca atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag
    11401 aagtacgagc ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac
    11461 agaaaaaaag cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt
    11521 ttttattgtc cttacattca taaagtttga atgcaatgac tttattacat ctatatttat
    11581 atctaacagt agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt
    11641 gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg
    11701 atgattgttt tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt
    11761 tttgtcttca aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca
    11821 taccagaaaa tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt
    11881 aacaagatca accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg
    11941 acttcactaa gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc
    12001 atccgccagt tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa
    12061 ataccgatag gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc
    12121 acaggacctt tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt
    12181 gataacgttg caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac
    12241 tgctgttgtt ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga
    12301 taagaacgct taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac
    12361 gaaaaataaa aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa
    12421 aaagaatggt aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa
    12481 gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag
    12541 ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg
    12601 ctagtactca gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga
    12661 tctgtgctgg attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg
    12721 taaaatatat tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa
    12781 gcttgattta ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga
    12841 cgaacttatt acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt
    12901 ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt
    12961 tgattgttaa attaatacca attgctgtga ttgtcatatg gggattattg accccaggac
    13021 aaggaactgt ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat
    13081 tgagcagtgc gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa
    13141 tggcagggga aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga
    13201 gttttgtaac agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg
    13261 atcatcttgc cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa
    13321 tcggtggaaa gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt
    13381 atacactaac tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca
    13441 gtaaacagct gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc
    13501 agttagcagt agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt
    13561 tgatttttgt gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa
    13621 aaaaagcgcc ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta
    13681 ttgcgattct cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag
    13741 cattgatcgg tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa
    13801 aacgctcaga agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct
    13861 agatcttcgt gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact
    13921 tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt
    13981 gattctcata cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag
    14041 cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt
    14101 gaaaagtctt tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat
    14161 ccgacagagg ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact
    14221 ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg
    14281 aaagaagtcc aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta
    14341 ccaatcagta ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa
    14401 gatgttcgag atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa
    14461 cgatttcttg acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca
    14521 ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat
    14581 gccccttatc tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt
    14641 tatacagtag aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa
    14701 acatggacaa atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca
    14761 agagattata gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc
    14821 agatacgata gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca
    14881 tgcccaagag actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat
    14941 aagaaaaatc attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca
    15001 agctaaacca aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt
    15061 agaagcattg aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac
    15121 tagacaagac cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga
    15181 aaaactaggt gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt
    15241 gaagatgttt cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca
    15301 acatgagtga atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac
    15361 atggtttttc atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa
    15421 ggaaaatcgt agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag
    15481 gaatcggtgc attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga
    15541 tcgatgatcg tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta
    15601 ttgtacatca agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg
    15661 aggagcttcc gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga
    15721 tgcattttct tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag
    15781 tagctgatcg tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg
    15841 ttcaatatta tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc
    15901 agccaaatgt agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag
    15961 tgacagacga aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa
    16021 cgctttggaa taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag
    16081 caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac
    16141 aagaattcgc cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt
    16201 gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    16261 ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16321 ttaagaaaga ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa
    16381 acagcttctc atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact
    16441 attatgccga acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg
    16501 atcaaaaaat tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat
    16561 gtgtcttgac agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat
    16621 attaggagag agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat
    16681 tgattgggat gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt
    16741 cctttacttt ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg
    16801 cagtggctgt tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg
    16861 cattgcgagc attgtcacat cttgtgtttg ggttgattgg ggcttggtac ttgcaaaagc
    16921 atccgagtat cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt
    16981 tcaattttgt gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt
    17041 actttattgg taatatgcct gaaacatatt attctcaagg atatttttat acagtattca
    17101 tcttgatggg gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta
    17161 tagcaggaac actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa
    17221 atcggaaaga gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca
    17281 aaaaaaaaac tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc
    17341 taaaaagagc tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt
    17401 ttgatgactt gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg
    17461 tcgactattt cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta
    17521 gaaccagttt ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct
    17581 gatttcacta cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct
    17641 ggacgaatac cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt
    17701 tttccttcag gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat
    17761 gtcacattaa agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg
    17821 ggtgtatcat aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata
    17881 acgattcggt cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt
    17941 gtttcaagac gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca
    18001 tctaaatttg ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt
    18061 cccaatgcca cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat
    18121 tctgtcagac ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca
    18181 tatttacgta atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat
    18241 gcgtagtttt ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact
    18301 tttccgccga tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa
    18361 gtagttgact ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt
    18421 gatagattaa aatcagttac agaataattt tctgcattgt catatttttt atatacgttt
    18481 ttcagagcca tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt
    18541 taaagtataa atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt
    18601 ttttttgtca atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat
    18661 acactggagt aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct
    18721 ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca
    18781 atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca
    18841 accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga
    18901 gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca
    18961 cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg
    19021 ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta
    19081 gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc
    19141 gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga
    19201 taatatgcga tataatacga acgtattgga taatataaac aataatatta gtttttcgta
    19261 tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac
    19321 agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga
    19381 cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa
    19441 ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt
    19501 attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa
    19561 aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc
    19621 agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt
    19681 ctttattgga tttacattga atgcatttat tgaaggagca atctacggat tctttttcta
    19741 tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat
    19801 caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg
    19861 ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc
    19921 catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca
    19981 cgctaaataa aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg
    20041 tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg
    20101 tgcccatttc gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg
    20161 tggacaggcg atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt
    20221 gttggatttt tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca
    20281 tagggggatt actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata
    20341 tctttaaccc ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt
    20401 catacatata agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc
    20461 caaaacgaag attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc
    20521 caaaactttt acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa
    20581 ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg
    20641 cccctgtttt tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt
    20701 cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt
    20761 tgatattttt gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat
    20821 cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc
    20881 cagaaagttc gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca
    20941 caccaacagt gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg
    21001 ataaataagc aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt
    21061 gtgcccgttg tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag
    21121 cttcttcttg ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc
    21181 ccgagatatc cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa
    21241 cgatccaact cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg
    21301 gacttagacg catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt
    21361 gattgaaggc cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga
    21421 cgatatttcc gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa
    21481 atgcatcttt tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga
    21541 tgagccattg actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga
    21601 acagtcgatt gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt
    21661 gtaacgtcat cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa
    21721 tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt
    21781 tttttacttt cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga
    21841 ttgagcaagt aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa
    21901 gatatgatgt gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc
    21961 aagataagtc tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa
    22021 agagtcatct aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg
    22081 ctgtttttgt ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt
    22141 tggaagctga cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt
    22201 ttctgtggcg tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac
    22261 agaagtttgg ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac
    22321 atccattccg tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg
    22381 tgtcaaagtc gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt
    22441 cagctgaaat gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac
    22501 atggtcaaaa gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc
    22561 aagaatcgaa ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg
    22621 tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata
    22681 attgatcaaa gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc
    22741 aactgtcagc aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag
    22801 ggcagagata ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa
    22861 cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt
    22921 ctgactaacg tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac
    22981 ggtttttttg atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa
    23041 cagacctact tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg
    23101 tgctctgatc aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt
    23161 tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt
    23221 attgattttt ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga
    23281 agcatagtat tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca
    23341 taagcccatt tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga
    23401 agccatcatt aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc
    23461 caaaatgatt tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct
    23521 cctattattt atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa
    23581 tttagaatga aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt
    23641 agaaattgaa tagactattt tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt
    23701 cattcatagg aacgtctatt ttacttaatt tttctataaa tgaattctgc ataaaaaata
    23761 tatcaagtaa atgtttatag atgaaactct atttacttct tttttaattt gttcttgcaa
    23821 tcttttttgc tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa ttgattttca
    23881 aagaaggagt atgaaaatga gaaaaatatt tattcgttta ttgtgggtac tatcttagag
    23941 gaataaataa tagtggagaa aactatggtt tggcgcagat tctatcaggt ttgatggaac
    24001 gaagacaacc tcccgacatc aagtcaaaag ctacttgaga ttttgatctg aaaataaaaa
    24061 aagaaaacaa ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa
    24121 ctaagagtgg tgtacgtaat caataaaaag gatggtgaca acagtacctc tttactctct
    24181 gaaagcatca taactcgttt ttcctcatta tgccaataaa ttgttgttat accacatata
    24241 aggatttgta atagctaaaa acagacagga aagttgaaat ctgtgaatct tattttgtat
    24301 ggaaggattt atagaacgca aaaatcaaga taaagatgaa taaaaatcct gtttcggcta
    24361 tatattgcgt atctacgcca ataaaacgag tttgttctgt catagccata taaagcaaag
    24421 gcaaaaatac aactacagcg agataagatc cgatgatttc tcgaaaagtt ttccaggaag
    24481 tatgtttcct gtttttatat ttatccgatt tcattgaata gtcatagcct ccttattctt
    24541 tcatatgttg aaattccagt ataaaaactg gaaggaactg tacgtttttt agaataaaaa
    24601 ttataaggga taagtaggct gctgtctata ataagatata tttttcaatt ctttattaaa
    24661 gattttattg acaaaatgaa tccgttttct atgatggagg aggggtgaat aagttgttga
    24721 agaaggaaat tttatgttac ttagagaacc agacagcctt tttcgattta gaacatatga
    24781 acgaggtctt tacagcacaa aggttagctg atcattttgg ggtaaaaaga aacacagtca
    24841 gtcattattt gaaccagtta aatgatgagc aactactagt caaaatcaat tctcgaccag
    24901 tgatttattt ccacaaagaa gcttttgaaa aacaattttt taagttacgc acgaattttt
    24961 atttttctat cgatgatctc aaagaggaac agcctttttt cgcgcaacca aaagacttat
    25021 tctcttttat gatcggatac gacgccagtt tgaaagaaag tatcgaacaa atcaaaacag
    25081 ctctttacta tccagatggc ggattaccac ttttgattac aggcgaaagt ggcacaggta
    25141 aaagttattt agttaatttg gtctatcagt attgtctgct tcatgatctg ctagaagatt
    25201 ctgcaccatt catcactttt aattgtgcac aatatgcaga taatcctgaa cttttgacta
    25261 gcaatttgtt tggacatgtt aagggagcgt acactggtgc agaagaaaat aaaaaagggg
    25321 cttttgaagc agcagatggt gggatcttgt ttttagacga ggtacatcgc ttaacaccag
    25381 aaggtcagga aaaactgttc acctatcttg atcaaggtgt gatttatcgt atgggagaac
    25441 ctaatttgtc acgccggatc aaaacacgtc tcttttttgc tacgactgaa gagattacta
    25501 gtcatttctt aacaactttc attcgaagaa tacctatccg aatcgaacta cctgcactta
    25561 atcagcgtag tcgtaatgaa cggctagaac tgatttactc tttttttctc gaagaacaaa
    25621 gaaaaatggg gagccctttg tcagtcagtg gaccagcttt gtcactttta acaagtcaga
    25681 aattatccgg aaacatagga gagctgaaaa atatcgtcaa agtttcagca gcaaaagcat
    25741 atgctgaaca gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg caaaaagaac
    25801 tacttgcgca gcctattact aaaaatacag cacagcaagg aatctatatt acgcaagacc
    25861 agaccttaga gcaattaatg gagaagcaac agcccgaaca gcagcgaatc gttaaaagtt
    25921 ttgagcaaat cctaattgat ttcaagaaaa ataactgtct gctatattct tgcgaaggaa
    25981 aattaaagca agaagtgatt caattatttg attttttgct gtttgagacc agtagacaag
    26041 aaaaacacga gctgctggtt tattggacac aatctatccg agagaccttt cgacaaatgg
    26101 aaactgccta tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt tactatctct
    26161 atcagcgaag cactatcaaa tggttaccgg aagaacatga tattatccaa ctaatcaaag
    26221 aattagaaca acaagtttct cagtcttatc catctagtta tcactatgtc caacgaattc
    26281 tggaattaag taagatcaaa ttagatattg aagacacaag tatggatcgg attttactga
    26341 cgatctatct aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa gcgatgattg
    26401 ctgctcacgg ttatgcaact gctagcagta tggcaaatgt agtaaatcgt ttattacaag
    26461 aggatctatt tgaatcattt gatatgccac tagatgtcac accacagcag attgcttcag
    26521 aaatccttga ttattgcgaa aacagcgatg tttcaaatgg attgatcata ttagtagaca
    26581 tgggatctct gaaagaaatc catcagtttt ttaaaaaaca gttatccgta ccgcttttga
    26641 ttttgaataa tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg caaaaaaatg
    26701 tatcattaga agagatagca gaagagactg ttcgacagat ccagccagaa tggcgactgc
    26761 tctatccaga ggaaaacaaa cccaaagcac tcattactac ttgcttcact ggaatcggta
    26821 cggcggtcca cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt cagttgaaaa
    26881 tcatcccata tgaatatcag caattaaaag ataagaaaaa cagtgaccca ttattttcca
    26941 tatacgaagt cgtaggaatg attggcacca cagatcctga tatcaccaat atcgactatc
    27001 tttcattaga agacctcatc tcgggggaaa agatgtctgt actagctgaa tggttggaca
    27061 ctactatgaa tgcttcagag aaggaaatat tcaatcagcg catcattcgt aatttttcat
    27121 tggaaaaagt actggattcc gtgactattc tagatacaga gaaggtgatg ggagaaatcg
    27181 atcattttat gagagaatta gaggtgcagt tgaatcgaag catcagcaat cccaaaaaat
    27241 tagcgttgta tgtccatgtg agctgtctca ttgaacgatt gatccgacaa atgccaatcg
    27301 aaacctatca aggattagag aaatggaagc agtgtcaaaa agaagggtta tctgctatta
    27361 aatctgcgtt tagtgtcatt gaagagcatt atagtgtcat gatccccaat tctgagattg
    27421 cctatatcta tgatattttg agtgaagata cagagtttct ctcgatagaa gaagaatttt
    27481 gaacataagc aagttgttgc cactaatttt ggcatgcaac ttgctttttt agtatgtgta
    27541 agtgaaaaga ggagatgaaa aagtggatag agaaattatc ttagcttccc atggaaaatt
    27601 tgcttcaggg atattagatt cattagagct gatctatggg aaaaatcatt ctattactgt
    27661 attcgattgc tatactgatg agaattttga tttagcggaa acagtaaggc agctttttat
    27721 gaggtacaaa gataaagaaa tcattgttct gacggatctt tttggcggaa gtgtcaacaa
    27781 cgaattctta caatatatca atcgggatca tgtctatctc gttgccggat tgaatttgcc
    27841 actattgatt gagctagtat ctcggttgga cgctgaagta gagacaactg ttttgatcca
    27901 gcaggtgcta actgattcaa aaaaaatgat ccagttttgt aacgagcggc tcaatcagaa
    27961 aatagaagaa gacgaatttt aggaggaaga aaaatgattg tattgacaag agtcgaccat
    28021 cggttattac acggacaagt agctttttca tggacacaaa caataggcgc agactgtatt
    28081 ttgattgcta atgatgatgt acctacgaat gagatacgaa agacaacgat caaattagcc
    28141 aagccacaag gagtcaaact agtgatcaaa tcaattgatg actcgattgc cgcgttgaaa
    28201 agcggggtga cagataaata taagttattc atcgtagtgg aatcgatcga ggatgcatat
    28261 aagctggcag aagcgtatcc agaaatcaaa accatcaact taggagggat caaggcaaaa
    28321 cctggtactc gcagcatcgg aaaagcggta aatattttac cagaagagga ggaacgcttg
    28381 aaacgattaa ttgacaaaca aatagaagta gaaatccgtc aagtaccagc agataaaaaa
    28441 gtggatgtaa gagaagtttt gtaagaaagg aagaacctca atgattgtac aagcagtctt
    28501 attaggaatt gttgcatttg tcgcacaatc agaatatgca ctagggacat cgttgctttc
    28561 tcgtccgatt gtgacggggc tgtttacagg aatcgtgtta ggtgatgtaa aaactgggat
    28621 cattatgggc gcaactttag agttagcatt tatcggatcg ttctcagtag gtgcttcgat
    28681 tccacctgat gttgtgaccg gtgggatttt agggacggct tttgctatta cagctggagc
    28741 cggtacagaa accgcgcttt tactaggttt gccgattgct acgttgacat tgatattgaa
    28801 aaatatttac ttagggcttt tgattccaat tatgaaccat aaagcagaca attatgcaga
    28861 agaaggaaat tataaaggag tcgagcatat gcacttgctt gccggtatcg gtctttcctt
    28921 gatgcttggt gttgtggtaa tggtttctta tatggtggga agcaatacga tcggaaatct
    28981 attaaatatg attcctgact tcgttcaaaa ggggttgtca gtagctacag ggttgattcc
    29041 agctcttggt tttgcgatgc ttgctcgttt gctgatcaac aaacaagttg caccatattt
    29101 tttcttaggt tttgctatag cttcttactt agagattcct gtgaccggag tagctatttt
    29161 tggtgcgatt ttagcagttg tggtagtcaa tattatgaat gtccgtcaac taccacttca
    29221 aacaactgca ggggaggtag aagaagatga agaattctga gatcaaaaac agcaaaataa
    29281 cgaaaaaaga gctaaatcac gtattttggc gttcttttca aatggagttt tcttggaatt
    29341 atgaacgtca gatgaatatg gcttatgttt atgcaatgat tccgattttg aaaaagttat
    29401 atcagtcgaa agaagaaatg gcagcagctt tgaaacggca tctagagttt tttaacacga
    29461 ccccacatat cgttacactg attttaggaa tcacggctgc aatggaggaa gaaaataagg
    29521 atgatcctga atttgatgtg actgcgatcg atagtatcaa aacatctttg atgggacctc
    29581 ttgcagggtt aggagattct ttcttttggg gaactttgcg attgattgcc acaggtgtgg
    29641 gtacctcttt agccatgcaa ggaaatattt taggccctat cttgttcatt ttgattttca
    29701 atattccgca cattctgttt cgctatctag caacaggttg gggatatcgc ttaggaacgg
    29761 gttttttgaa aaagatccag gcaaacggta tgatggaaag tttgacatta ggcgcgtcta
    29821 ttattggact gatggtcgtt ggagcaatga ctgccacgat gatcaccatc aatattcctt
    29881 taaaaatcgg ttcaggagaa aatgctgtga ctgttcaaag tatttttgat gatatcgttc
    29941 caaatatctt atgtcttgga gcatttggca tagtgtttta tctattgaag aaagaagtga
    30001 aaccattaac gatcttgatc ggactagctt tctttggaat ttttggttcg tggataggga
    30061 ttttttaaaa aaggagcaga gaaaatgttt acgatgcaag attatatcta tgaagaaaag
    30121 gaagtactaa gtacgatctt gaaaaagaat gatttttcga caagagaaca tatgaaaaaa
    30181 acagtgaatt tactgatctt agcaacgggt tcatcttata acgcttgttt agcagcgaag
    30241 ccagcattgg aatcttatgg tgatcttact gtcgatattc aagaaccttt ttattttttc
    30301 aattatggaa aactttctcc ttcaatcgat actgtgattg ctgtgtcaca aagcgggaaa
    30361 agtgcttcta ctgttgaagc agtaaaaatg atacaaaaac aagggttacc tgttgttgct
    30421 atcacaaatg acgtgcaaag tccacttgct ttagaagctg cacaaattat tgatttaggt
    30481 gccggggtag aatcagtagg cttcgttaca aaaggctatt ctgcgactgt tttacagtta
    30541 ctactcttag ggataggaat tggaataagt aaaaataaaa tcagcaaaaa aatagaacaa
    30601 gactacatgc aacagttgcg caaaataatc aaccatttac cagcgattat tcaaaaaacg
    30661 gaagaatttt ttgatggata tcaaagttta tttcgtttag cacagcgttt cgtcaccatt
    30721 ggatatggtc caaactgggg aacagctaaa gaagcagaga cgaaattgac tgaaacaatc
    30781 cgcgttccgt cacaaggatt tgagttagaa gcttatatgc acggcccata cctggaggca
    30841 gatgcttcgc atttgctatt ctttattgaa ggggaaagtg taaataagga acgttcgcaa
    30901 aagttgcaaa gatatatgag tcggtacgtt ggagagactc tcaccattac cacaaaaaaa
    30961 gcaagaaacg agaaaacact tggattagca atagaatgtg atgaatatct gtctgtatta
    31021 gcgcttgtcg ttccgtttca actattcgct tataaaaccg cagtagctaa aggtattgac
    31081 ttgaacaaaa agatttttga agactttgat acagtcttga aaagtaaatt ataagaggtg
    31141 gaaatcatgc tgaaattcaa tgaagaagaa caaatcaaag caataaaagg cgcactagct
    31201 ttacgtccgc aagtagaaga aatcatcgat aaactttatg cggaaaaatt tgacgctgtc
    31261 tattatctag gaatcggtgg aacgtatgct tcctcgatgc aagctgtcac ttatatgaat
    31321 gggaaaagca acttgcctgt ctttgtacag catgctgcag aatattacac tactggaaac
    31381 aaaagattaa caaaagattc tatcgttatt ttgtcctctg ttaccggtac aactcaagaa
    31441 gtagtgaaag cagtagaaca aatcaagaaa gtaggagcga cactgattgg atttatcgat
    31501 aaagcgaaca gtaagttgtc tcagctatgc gattttgtgg tcacttatcc agctcctgga
    31561 acagaacaaa tcaaattttt tatggcagca gatcgattga tgtatttgaa tggagaattc
    31621 gaagcttatt cagaatacta tgaacagctc gaacgttatt taccaacagg gttagtggaa
    31681 gcagagaaaa aagcagatgc atttggctta tcatttgcag aaaaacatcg acgtgatagt
    31741 atgcattatt ttattggagc agggaatcaa tggggagctg tttattcata tgccatgtgt
    31801 tactgggaag aacaaagctg gcttccatcg aaatctatcc atgctgccga gtttcttcac
    31861 gggacgttag agatcgtaga agaaacaacc ccagtcacat tatttttagg cgaagatgaa
    31921 cagagagtgt tatctgaacg tgtggctaaa cttttgccaa aaatttgcag caattataca
    31981 ttgatagaca caaaagacta tccagtagaa ggtatcagtg aaaaatatcg cggacgtgtt
    32041 ttgtctttct tattgatgca tgtagtgaca caacgcatcg atgctcatgt ggagcagttg
    32101 aactgccatc cattagagat acgccgctac tatcgtcagt ttgattatta ggatagactg
    32161 gataaaaaga atataaaaaa taaagagagc agcgataact gctcccttta tttttttaaa
    32221 atattggcat caattacgtt ataaaacaca ttttttgcat tatctacagt tcaacttata
    32281 atctgtttaa tgggacatgt gggtgccaac cattttttgt tatgcaatag tactatttag
    32341 ttgttgcatg gatccgtagt aaaagtccaa gtacaacgag ttgcgccagt ttacatagcg
    32401 ttttgtatcc agaatgttga ctaaaacaaa agttcctatg tcaaccgtca acagagacaa
    32461 tctgtccgtt gcaggacctg acagatttag aaatgtccaa tctgtctctt atgtatttta
    32521 aatcaatact cactaggaga agtttcctaa cctcctaata gtaatgctgg tagtaacatt
    32581 gtcaaaactg cttcctctta ggaacaagca aagcataacg agtgtaaaaa taaaatgtag
    32641 actattgtaa catatcattg attatttcaa gtaaaaacga taaaagaagt aaacaaacac
    32701 ttaaaaacaa tggaaattag tcccaacttt aagaaatgta tactataatg gaaattatca
    32761 attatttatt tggaggaata tccatgaaac aaaaaatcgc agttcttggt cctggatctt
    32821 ggggcactgc tttagcacaa acattggcag aaaacgggca tgatgtccgt atttggggaa
    32881 atgtacctga acagattgat gaaatcaata agtatcatac aaatcagcat tttttacctg
    32941 atttgacgat cccagaatcg attatcggat ataaagaatt ggcagaagca gtggataacg
    33001 cagatgctat tttatttgtt gttccaacaa aagcgattcg ttcagtcgca caggaactta
    33061 ttggtaaaat gaatactaag ccaatcatta tacatgcaag taaaggacta gaacaagata
    33121 cgcataaacg aatctcagaa gttttggctg aagaaattcc agaagaaaaa agacaggcaa
    33181 ttgttgtatt atctggtcct agccatgctg aggaagtggc agtacatgat atcacaacaa
    33241 ttacagctgc aagtattgat caaaaagcag cagcctatgt acaaaaactg ttcatgaatg
    33301 attatttcag gatttataca aataatgatg tgattggcgt agaaactggt gcagcactga
    33361 aaaatatcat tgcaattggt gccggcgcta tccatggatt aggattcgga gatgatgcaa
    33421 aagcagcaat catgactcga ggattggcag aaatcagccg tcttggtgtc gcgatgggtg
    33481 ccaatcctct gacatttatc ggtttgagtg gtgtggggga cttgatcgtt acttgtacaa
    33541 gtgttcattc tcgcaattgg cgtgccggta acttgttagg acagggacat aagctggaag
    33601 aagttctaga aaacatggga atggtcgttg agggtgtggc aacgacaaaa gcagcagtag
    33661 agctagctca acagctaggt gttgagatgc caattactca aacaatctat aatgttttgt
    33721 ataatggaga agatatcaaa aaagcagcaa aagaaatcat gttacgtgac ggaaaaatgg
    33781 agaatgaatt ttctcttcgt taaatagatg atcaatagtt aatcgtc
//
LOCUS       DACEDO010000026        32747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEDO010000026 DACEDO010000000
VERSION     DACEDO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.026"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            463..1152
                     /locus_tag="IWO52_001292"
     CDS             463..1152
                     /locus_tag="IWO52_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0355570.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1139..2347
                     /locus_tag="IWO52_001293"
     CDS             1139..2347
                     /locus_tag="IWO52_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0355571.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2641..3912
                     /gene="serS"
                     /locus_tag="IWO52_001294"
     CDS             2641..3912
                     /gene="serS"
                     /locus_tag="IWO52_001294"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0355572.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4461..5945)
                     /gene="guaB"
                     /locus_tag="IWO52_001295"
     CDS             complement(4461..5945)
                     /gene="guaB"
                     /locus_tag="IWO52_001295"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0355573.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6450..7145)
                     /locus_tag="IWO52_001296"
     CDS             complement(6450..7145)
                     /locus_tag="IWO52_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0355574.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7235..8335)
                     /gene="ychF"
                     /locus_tag="IWO52_001297"
     CDS             complement(7235..8335)
                     /gene="ychF"
                     /locus_tag="IWO52_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0355575.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8344..8535)
                     /locus_tag="IWO52_001298"
     CDS             complement(8344..8535)
                     /locus_tag="IWO52_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0355576.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8556..9446)
                     /locus_tag="IWO52_001299"
     CDS             complement(8556..9446)
                     /locus_tag="IWO52_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0355577.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9433..10200)
                     /locus_tag="IWO52_001300"
     CDS             complement(9433..10200)
                     /locus_tag="IWO52_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0355578.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10212..10925)
                     /gene="rsmG"
                     /locus_tag="IWO52_001301"
     CDS             complement(10212..10925)
                     /gene="rsmG"
                     /locus_tag="IWO52_001301"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0355579.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11157..13058)
                     /gene="mnmG"
                     /locus_tag="IWO52_001302"
     CDS             complement(11157..13058)
                     /gene="mnmG"
                     /locus_tag="IWO52_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0355580.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13075..14472)
                     /gene="mnmE"
                     /locus_tag="IWO52_001303"
     CDS             complement(13075..14472)
                     /gene="mnmE"
                     /locus_tag="IWO52_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0355581.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14816..15154
                     /locus_tag="IWO52_001304"
     CDS             14816..15154
                     /locus_tag="IWO52_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355582.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15322..16080)
                     /locus_tag="IWO52_001305"
     CDS             complement(15322..16080)
                     /locus_tag="IWO52_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0355583.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16098..16916)
                     /locus_tag="IWO52_001306"
     CDS             complement(16098..16916)
                     /locus_tag="IWO52_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0355584.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16913..17269)
                     /gene="rnpA"
                     /locus_tag="IWO52_001307"
     CDS             complement(16913..17269)
                     /gene="rnpA"
                     /locus_tag="IWO52_001307"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0355585.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17387..17521)
                     /gene="rpmH"
                     /locus_tag="IWO52_001308"
     CDS             complement(17387..17521)
                     /gene="rpmH"
                     /locus_tag="IWO52_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0355586.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18044..19378
                     /gene="dnaA"
                     /locus_tag="IWO52_001309"
     CDS             18044..19378
                     /gene="dnaA"
                     /locus_tag="IWO52_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0355587.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19576..20706
                     /gene="dnaN"
                     /locus_tag="IWO52_001310"
     CDS             19576..20706
                     /gene="dnaN"
                     /locus_tag="IWO52_001310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0355588.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20935..21177
                     /gene="yaaA"
                     /locus_tag="IWO52_001311"
     CDS             20935..21177
                     /gene="yaaA"
                     /locus_tag="IWO52_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0355589.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21164..22288
                     /gene="recF"
                     /locus_tag="IWO52_001312"
     CDS             21164..22288
                     /gene="recF"
                     /locus_tag="IWO52_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0355590.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22285..24231
                     /gene="gyrB"
                     /locus_tag="IWO52_001313"
     CDS             22285..24231
                     /gene="gyrB"
                     /locus_tag="IWO52_001313"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0355591.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24251..26722
                     /gene="gyrA"
                     /locus_tag="IWO52_001314"
     CDS             24251..26722
                     /gene="gyrA"
                     /locus_tag="IWO52_001314"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0355592.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26958..27257
                     /locus_tag="IWO52_001315"
     CDS             26958..27257
                     /locus_tag="IWO52_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0355593.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27307..27819
                     /gene="ssb"
                     /locus_tag="IWO52_001316"
     CDS             27307..27819
                     /gene="ssb"
                     /locus_tag="IWO52_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0355594.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27845..28081
                     /gene="rpsR"
                     /locus_tag="IWO52_001317"
     CDS             27845..28081
                     /gene="rpsR"
                     /locus_tag="IWO52_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0355595.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28224..30197
                     /locus_tag="IWO52_001318"
     CDS             28224..30197
                     /locus_tag="IWO52_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0355596.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30203..30655
                     /gene="rplI"
                     /locus_tag="IWO52_001319"
     CDS             30203..30655
                     /gene="rplI"
                     /locus_tag="IWO52_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0355597.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30929..32296
                     /gene="dnaB"
                     /locus_tag="IWO52_001320"
     CDS             30929..32296
                     /gene="dnaB"
                     /locus_tag="IWO52_001320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0355598.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         9954 a         6400 c         6457 g         9936 t
ORIGIN      
        1 tatcataact tatcgtttgt tatttgtcaa gaactttttt gaagtttttt tcgatagaat
       61 caagcttcgc aagtggttct tttttaacga ctcagttatc ttatcatgtc atttccaaca
      121 tgtcaacaac ttatttttta ttatttaata agttattggt gttcttttga acaaataata
      181 atatatcaac atcacatgtg aaagtcaata agaaaatgct tatttatcaa catttctagc
      241 ttacatgatc attggttagt tctggtcctt tttcgttcgt ctttataact ccgagagacg
      301 taaaatcctt ttgttttcag tttaatgttc atgttcttgt cttattcaca ccaactagat
      361 gtctcacctt ctaaaatttt ccttaacaaa gtgctgcttt tatctatttc tgcttttttt
      421 tgctagaata atagtaacaa tatgactctg gaggatttat ttatgaaaat tttgattgct
      481 gacgatgata aagaaatcgt tgaattatta agtatctatg ttcacaatga ggggtatgaa
      541 gcagtaaagg cttatgatgg gaaagaagca ttatctaaaa tcagaacaat ccctgatatt
      601 gaattactta ttttagatat catgatgcct gagatggatg ggatgcaagt agtgaaagag
      661 ctacgaaaag aatcccaaat accaatcatt atgctgacag caaaaactac tgatatggat
      721 aagatcaaag gactggtcgc tggtgcagat gattatgtga ccaagccatt taatccgtta
      781 gaagtaatgg ctagagtaaa gtctttatta cggcgtacaa aaatgcagat tgccccagat
      841 cagcctgatg tattagaagt aaatgcatta atcatcaata aagactctca tgaagttaaa
      901 acaatcgatg ggaaagaaat ccagttaaca gctttggaat tcggtatcct ctacttgctt
      961 gctagtcatc ctaatcgagt atttagcgcg gatgaaattt ttgagcgtgt ttggaaacaa
     1021 gaaagtattg tctctgctaa aactgtcatg gttcacgtta gtcatttaag agataagatt
     1081 gaagaagcaa ctggtggtga aaaagtcatt caaaccgttt ggggagtggg ctataaaatt
     1141 gacgcaagat agtaaacgag caactgaaaa acgccgtcgg atcaatttga cttcaaaaga
     1201 aatcagcgaa ttgttagcgg aaggaatcat cacgattatc cttttgcttc tattaaatgt
     1261 ttctattctg gttgttatca gttcggtgat caacagttca ccttctttga cgaatgcgat
     1321 ttgggattca aagaatattt ttgcagaacg attgaacact gatttgttct ggaacggccg
     1381 gaattttatt attccctttt tctttctatt agatattggt gttttgtatt ggcgcttgat
     1441 tcgaagatat cgccaaatgc aattgcggca tatcatcagc gaacttcact acatcgcaaa
     1501 cggaaattat gatcatcgta tcccttttga actgagtggt gacttaagcc gagtggtaac
     1561 aagtatcaat ggattggtgg atagtaccgt agcagcgatt gaagacgaac gtaaaatcga
     1621 aaaatcaaaa gatgaattga tcacgaatgt cagtcatgat atccgtaccc cactcacgtc
     1681 catcattgga tacctaggac tgatagaaga tggccagtat cactcagaag aagatctttt
     1741 aaagtatacg catactgctt atatcaaagc aaagcagatg aaatctttag tggacgactt
     1801 atttgaatac actaaggtac gccagcctgc tgtcccggtg aatttttctg cctttgacat
     1861 gatccagttg atcgaacagt tagcagctga ttttgagttg gaagcttcta aaaaaaatat
     1921 ccaaatactt gttcaatcaa aagtagactc ccttatcatg gatggcgata ccgaaaaact
     1981 tgtacgtgtc ttcaataatc ttttgacgaa tgcgttgaaa tacggaaaag gagcaacgaa
     2041 gatcgtaatc gaagtagaac gtatcggttc agaagttgtt gcaacagtca aaaataacgg
     2101 agcaatgatc ccacaacagg caatcgataa tctattcgac cgtttttatc gtgtagaaga
     2161 atctcgttca caagcaactg gaggaactgg acttggttta gcaatcgctc aaagcattgt
     2221 ggctttgcac ggcggataca tttatgcaaa atcagataag caatggactt ctttcatcat
     2281 gcaccttcca atcaagaaaa atgaaaaatt acctattgat acacaggaag ttattgacga
     2341 aagctgaaat attcgctatt cttattacaa ttattagaat aaatgaaaaa cacagaaccc
     2401 agtacacttt attctttttc agagagctga tggcaggtgt aaatcagcaa aggataattt
     2461 ggaagttcca gttcgtttac gtgccctttc gaaataagaa aggtcggttc aacagccgtt
     2521 atgtgttcaa caagtgcagc tgacgctgaa cttgggtggt accgcgaatt tcagactgat
     2581 tcgtcccaag aataatggga tggatcagtc tttttttatt ttcaaaaagg aggaaatcat
     2641 atgttagatg taaaaatgat gcgacaaaat attgaagaag tacaagaaaa attgatgact
     2701 cggggcgtaa aaaaagaaac attagctgaa ttcatggaac tagatgaaaa tcgtcgtaag
     2761 ctactagtca aaagtgaaga attaaaaaaa tatcgaaatg atgtttctgc tgagatcgcc
     2821 caattgaagc gtgcaaaaga agatgcttct gctaaaatcg ctgaaatgaa agaagtcggt
     2881 ggcaacatca aagcattgga tactgaaatt gctgaagtgg atgaaaaatt gaaacaaatt
     2941 gcaactactt tgccaaacct tccccatgat tctgtgccag ttggaacaga tgaagaggac
     3001 aatgtagaaa tccgtcgttg gagcgaacca cgtacatttg cttttgaacc aaaaccacac
     3061 tgggaaattg cagaaaatct tgatatttta gattttgaac gtggcgcaaa agtagccggt
     3121 agccgttttg tctattacaa aggattaggt gctcgattag aacgtgctgt ctacaacttc
     3181 atgcttgatc agcatattta cgaacacggc tatactgaaa tgatcactcc ttatatcgtc
     3241 aacagtaaag cgatgtttgg tacgggacaa tttccaaaat tcaaagaaga tgttttccaa
     3301 ttggagaata ctgatctaac attaatccca acagctgaag taccattaac taactattat
     3361 aatgatgaaa ttttagatgg tgcagattta cctatttatt tcactgcact aagccctgct
     3421 tttcgttctg aagccggaag tgctggtcgg gatacacgag gactgattcg tcttcaccaa
     3481 ttcaataaag tagaaatggt caaattttcg gatgtagagc attcttacga agaattggaa
     3541 aaaatgacag ctgatgcaga agatatcttg aaaaaattgc atttgcctta tcgtgtgatg
     3601 gccttatcaa ctggagacat gggcttttct gcagctaaaa cttatgactt agaagtatgg
     3661 atacctgcac aagatactta tcgtgaaatc agttcttgtt ctaactgtga agatttccaa
     3721 gcacgccgcg caatgatccg ttatcgcgat aatgatggaa aaatccacta tactcatact
     3781 ctgaatggtt caggacttgc agttggccgt acagtagctg ccattttgga aaactaccaa
     3841 aatgaagatg gttctgtcac agttccagaa gcgctcgttc cttatatggg gaatctgaaa
     3901 gttattaaat aaagaggctg tgaaaaagcc gcttagtttc gagtattaag aaaaattttg
     3961 tgaaaatggc tttccacatt tttgcataaa ttggacttaa taccgagaaa ctggcttttg
     4021 aacaccattt atcgagaggt tgtgaaatca gcgaattaac ctgagtatta gagaaaaaaa
     4081 tggaaaaata gtccctcata ttttaccgtt tgttgaacta ataccgaggg tcagctgatt
     4141 gaacaccgtt tagtggagag gtcgtaagcc taccccccag ctcctagtta caaggacaaa
     4201 ttcaaaaaac agctcttcat attttatgaa tgttgtactt gtgattcgga ggtggcaagg
     4261 gaacactgtt tattcaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
     4321 aaattcacga aaattgcttt tcaaattttt gtgaatttct tcttatttcc gaaggagttg
     4381 cttctacttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
     4441 tcccacgctc tttaatgttt ttaagattca actgaatagt ttggtgcttc ttttgtgatt
     4501 tgtacatcat gagggtgtga ttcttttaat ccactgccgc tcatttggat aaattgcgct
     4561 tcatcacgta attgctgtag attggctgcc ccaacataac ccattcctga acgcaaaccg
     4621 ccgatcattt ggaaaataat atctgcaaca cttcctttat aagcaacccg accttcgatt
     4681 ccttctggta ctaatttatt tgcttcattg acactacctt ggaagtaacg gtcttttgaa
     4741 cctttttcca tcgcgcctaa tgagcccata cctcggtatg ttttgaatcg acgtccttgg
     4801 aagatttcga attcacctgg tgattcatct gtccctgcca acatacttcc tagcatcact
     4861 gcatgtccgc cggcagctaa tgctttgacg atgtcaccag aatatttgat ccctccgtct
     4921 gcaataatcg ctttgccata ttgacgagca actgatgctg catcataaat agctgtgagc
     4981 tgaggtacac ctacaccggc taccacacgt gtcgtacaaa tcgatcctgg tccgatacca
     5041 acttttacga catctacacc tgcatcatat aaagcttttg tcgcttcagc cgtcgcaacg
     5101 tttcctgcaa tcaatgttgc atctgcaaaa gttgaacgga tttcttgaat ttttcggatc
     5161 acacctgcac tatgtccatg agctgtatcg atgatgattg catctgcccc tgcttctaat
     5221 aaagcattcg cacgctcaaa tgtatcgctt gttacaccaa cggctgctgc tacaagcaat
     5281 cgaccatgtt catcttttgc tgcatttgga aattcgatca ctttctcgat gtctttgatc
     5341 gtgatcaatc cgcttaaacg accttcgtta tctacgattg gtaatttttc gattttatgt
     5401 ttttgcaaaa tcttttctgc atcttttaga gaggtaccta ctggggcagt cactaagttg
     5461 tcttttgtca tcacatcagc aatcggcatg tgataatctg ttacaaaacg catgtcgcgg
     5521 tttgtaatta tccctaccaa ttttctgttt tcaagtgttt caacgattgg cacaccactg
     5581 atacgatatt tgctcatcaa ttcttctgcg tctgctacaa gatgctgtgg cgttaagaag
     5641 aacggatcaa tgattacgcc actttcagaa cgtttgactt ttcttacttc atccgcttgc
     5701 tggctgatcg tcatgttttt atggatcaca cctagtccgc cttgtcttgc catagcaatg
     5761 gccatcttgc tgtcggtaac agtatccata cttgcactga tgattggaat attcaactta
     5821 atatttttag ctaattgtac gctcatatcc acttcgtttg gtaacacgtg actttctgct
     5881 ggtatcaata agacatcatc aaaagtaaat ccttttttcg tgaactttgt ttcccagttg
     5941 gacattttta gtaccactcc tctaatttag tttatctatt aaaataattg attttttcat
     6001 ttaagtcaat gatctacttg gaatcaagtt agctttctca tagaatacca atataaagcg
     6061 tttacatctg tgtgtattaa aagagattgt aaaaaagctg tcctgcatca agtaataaga
     6121 acagccaatc aaaaacagct tctcatgttt ttgaattggt tgggtttatt accgcagatg
     6181 caaacttttg aacaccgttt agtggagagg ctgtgaaaaa gctgcttagt ttcgagtagt
     6241 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaattggact taataccgag
     6301 aaactggctt ttgaacaccg tttatcgtaa aaagggactg tgacaagact tttgtcacag
     6361 tcccttacta ttatttaact atggataaac aaaatgaggt tctttactac ccctagatat
     6421 ctagcaaaat agctgatctt tttgctagtt tatcttcttg ggacggataa acttccaata
     6481 atcccgtatt ttttcttcaa gtagtaacgg attgctaaag cagttgcccc gataatgata
     6541 actactactg gatctaatac tggattgata actggtggca atagtgttgt cgcagaaaac
     6601 agtacgaccc aaagaagtgt cgttccacct aggataagca atgtcttacc cattcctggt
     6661 ttcttgcttc gatctgctcc gggatattca tattgataga tgtatttgta catcatataa
     6721 aagacccagc caccgattgc agaacctaga acgagtgtag aaaagccata agctggcgtt
     6781 gttccacgtg aaaacaatcc ggtaatcgca atcatgatac ttaatagacc aagcaacagc
     6841 aacgtattat ctaaccacat caataaaggg gtggatgaat tttttacttc tgctggctta
     6901 ttgatgattg cttccgttct ttcagagact gtcccaaaca attgacgggc tgttcttccg
     6961 cctttttgtt ctgcaactaa agtaggcaga atgtcatgaa gggcttttac tttttcttct
     7021 tcagacaaat tcgctgcttc taaagatttt ttcaaatcaa aaatgtactg ttgatttctt
     7081 ttggttaatt ttgtttctaa actattgttt tcttttacga ttgctcgtaa ttgttctggt
     7141 tccattatct aatagtcctc cttagccgtt ccttttctca acggtcgtca caaaagacat
     7201 tctaccatat tctacagcta gtttgatagc ccttttacac attaaagcgg aataacatta
     7261 cgtcgccgtc ttgtacgaca taatcttttc cttctaatcg aacacgtcca gcttcttttg
     7321 ctgcatgcat gtttccgtaa tgatccaagt cttcaaaaga gacagtttct gcacggataa
     7381 atccgcgttc gaaatctgaa tggatgattc ctgctgcttg tggtgctttg attccttttt
     7441 tgaatgtcca agcacgtact tcttgttccc cagcagtaaa gtaagtagct aagcctaaca
     7501 aatcataagc agcacggatc aactgatcca aacctgattc ttcgattcct aaagcttcta
     7561 aaaattccgc tttgtcttct tcatctaatt ctgcgatttc ttcttctgca cgagcactga
     7621 ccacgatcac ttctgcattt tcttctgcag caaagttgcg cacttcttgt acataatgat
     7681 tcgcgtctgc atctgctacg tcatcttctg agacattggc tacataaaga attggttttg
     7741 tcgtcaataa aaatagtgat ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc
     7801 gggcagattt tccttcttcc agtacaggtt tgattttatc cagtacagct agttctgcca
     7861 ctgcatcttt atctttggtt ttagcaactt ttgctacacg agtatagcgt ttgttgatag
     7921 attctaagtc tgctaacact agttccaagt tgatcgtatc aatatcttct agtggatcaa
     7981 cacgtccttc tacgtgagta atattatcat catcaaaaca gcgaacaacg tgacaaatag
     8041 cgtctacttg gcggatatgg cttaagaatt gatttcccaa accttctcct ttacttgcgc
     8101 cttttacgat tccagcaata tctgtaaatt caaaagtcgt cggaacagtt tttttcggat
     8161 gtacaagttc tgttaagcgt tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg
     8221 ggtcgatcgt cgcaaaggga tagtttgctg cttctgctcc tgcttttgtg atcgcattaa
     8281 acaaagttga ttttcctacg ttgggcaacc caacaattcc agcagtcaat gccataaatt
     8341 tattcactct ttctctgcat ttttttcaat tacttttttt aatttctttt caaaatctcg
     8401 acgggtcatc atgacgatat gtccgcaatt catacatctg attttgatat ctgcgcccat
     8461 tcgaatgact tcccagcgat tcgtctggca agcgtgaggt tttttcattt caacgatatc
     8521 gtgcaagtcg tacataaaaa tagcctcctt tttgtttatt cttcatcaaa ttgaatattc
     8581 aagatatcaa gaattcgagt caaatctttt tgagaaagat attcaatctc gatcttacct
     8641 ttcccatttt tttcttggat agctacactt gtaccaaatt tatccatcaa acgatcttcg
     8701 ctttctcgga tataatatgg tttttccttt gctagccttg ggattttttt cttttctttt
     8761 ccatcatttt gatttaactc agaaacaagc tgttccagtt ggcggacagt taaattttca
     8821 gttactgcac gattggctaa tttcaagatc aatgttttat tttttaaacc taacagcgta
     8881 cgggcttgtc ccatagataa acgctgatct tgtaccattt ccttgacaag atccggtaaa
     8941 gaaagcaagc gcaaataatt ggcgatataa ggtctgctct ttcctaaacg actagctact
     9001 tctgcttgtg ttagtttcag gtttttcatc agcatctcat atgcttctgc ttcttctaac
     9061 ggattcagat cttctcgctg caagttttcc agcactgcta cttgcatcat cgcttcttcg
     9121 tcaaagtctc taacgatagc agggatcgtt tctttttcag ctagtttgga cgctcgaaaa
     9181 cgtcgctccc ctgcaataat ctcatatcct tttactgcgg aacggcgtac gatgatgggt
     9241 tgaaagacac ctgattgttc aatagaatta gctagttcct gcaaagatac ttcttcaaat
     9301 gttttacggg gctgatacgg atttggtcgc agctcattca atggtagctg gacaacggta
     9361 tcatctttta catccaccgt ctctaagttt tctaaatctt gaaacagtgc atcgattcct
     9421 cggcctaatc ctttactttt cttcacgact taccacttcc tttgccaatg cttggtagac
     9481 ttcagctcct cgagaacgtg gatcgtagtc gatgatcggt tttccatgac taggtgcttc
     9541 tgataaacga atatttcttg gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg
     9601 tacttcttct acgacttcat ttcccagatt tgttcgtgca tcatacattg tcagtaagac
     9661 tccctcgatc tctaattctg gattaaagtg tttttggact aaacgaaccg tattcagtaa
     9721 ttggcttaat ccttccaatg cgtaatactc acactgaaca ggtatcaaaa tcgaatcact
     9781 tgctgtaaat gaattgattg tcagatggcc aagagaaggc gggcaatcga tcaaaatata
     9841 atcatactga ctgcttactt ctgctaaaga tccttttaat cttgattctc ttgccatcat
     9901 tgacgtcaat tcgatctctg ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt
     9961 ttcgtgttca gtgatcaaag ttgcctcatc aattggcaat tcattgacta gaacatcata
    10021 gatgtctctt gtcacatcag gttttctgat cccgacacca ctggtagcat ttccttgtgc
    10081 atccatatcc actagcaata cttttttgcc aaggctagca agacaagcac ctagattcac
    10141 cgtcgtcgtt gttttgccga cgccaccttt ttggttcgca acagatatta tttgtgccat
    10201 ctcttctcct cctatttgat tggctgctta ttaggcagtc cgggctttct tgggtatttc
    10261 tttggggttg gtttcttttt ttgaataacg atgatatgcc gttgatcaga gacattcggc
    10321 aaggtaaccg cctcttcttt gaggaattta ccacctaata aagcaatcgc ttgttttgct
    10381 tctgtcaatt cctcttcact ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta
    10441 gccaagggca aacacaattc cgctaacaca ttcaaacgag caaccgcacg agcagtgaca
    10501 aggtcaaatg atgcacgaaa gtgaggattt tgcccaaatg tttctgcccg gtcatgatat
    10561 aaggaagcat cgattcctaa taatcgggtt aactcagata agaaggttat tcgtttattc
    10621 agagaatcaa cgatcgttat ctgcagttct ggaaaggcaa tttttaaagg tatgctagga
    10681 aaccctgccc ccgaaccaac atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat
    10741 gctaatgtaa gtgaatcgta aaaatgcttc agatatacgt ctttttcatc cgtaatagct
    10801 gtaaggttca tcttttcgtt ccattcgact aatgtccgat aataaatagc gaattgattc
    10861 atttgatgat cggtaagctc tatccctttt tctgccaagg attggcgaaa ttcttctggt
    10921 atcataaata ttcctttcat tgtgaaacag caattgtttc acagattctc tccctacttt
    10981 aacgcaaaat ctctagacat gcaatccttc catatggatt cttaaaaatt tgaaaagtaa
    11041 gaaaagccga tagacttttt ctttctagta tgactcataa aatatacaca gaagagagaa
    11101 ctagctttga gcaatttctg caaaaactag ttctctcttc tatttattat tacgtattac
    11161 gctggttcac tgactttcgc aatcttccct tgttcgatat agaccattag gatacttagg
    11221 tctgcagggt ttacgccact gattcggcta gcttgggcaa ttgtttctgg ttggattttc
    11281 tgcaattttt gttttgcttc tgttgctaat ccgttgattg ctgcgtagtc gatgttttct
    11341 ggaatctttt tcgcttccat gcgttttaat ttttctactt tttccatggc ttttttgatg
    11401 tagccttcat atttgatttg gatttctact tgttcgattt cttttcgtgt caattcttca
    11461 ttttctggaa taaaccgtag aagttctgca taagtaatct caggacgtct taaaaagtcg
    11521 cttgctagta caccatcttt taatggtgcc gcattaatcg ttgctaaaaa gtcttggact
    11581 tcttctgttg gtttgattcg tacattcatc aaacgtttga tttcttgttc aactgcttct
    11641 tttttcttca aataattctt gtattgatct tcttttacta agcctacacg gtgacccatt
    11701 tctgttaatc gcaaatctgc attatcatga cgtaaaagca gacggtattc agcacgtgaa
    11761 gtcagcaaac gataaggttc gttcgttcct tttgttacca aatcatcgat catgacaccg
    11821 atgtaaccgt cacttcgttt gatcactaaa ggttcttttc cctgaacttt caaagctgca
    11881 ttgataccgg caatcagccc ttgtccagcc gcttcttcat aaccgcttgt accatttgtt
    11941 tgtccagctg tataaagatt ttcaacgact ttagtttcta atgtcggacg gagctggtgt
    12001 ggctctacta cgtcatattc gatagcatat cctgtacgca tcatttctac tttttctaat
    12061 ccagcaatag aatgaagcat gttgacttga acatcctctg gtaaagaagt agataaacct
    12121 tgaacataga cttcttccgt atttaagcct tctggttcca aaaataattg atgacgtggt
    12181 ttgtctgcaa aacgcacgat cttatcttcg atggatggac aatagcgagc gcctacccct
    12241 tcaacgatcc ctgtaaacat tggggcacga tgtaagttgt ctcgaataat ttggtgagtc
    12301 gtctcacctg tataggtcag ccagcaagat tcctgatcta gtttataggc actatctggt
    12361 gtgctgaagc taaaatgatt cggctctttg tcaccaggct gctcttctgt aacagaatag
    12421 tcgatcgtac ttgattttac tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag
    12481 ccaagttctt ttagatgatt cgctagtcca atagaaggtt gagagttatt cggacctgat
    12541 gaatatttca attcaccgat aatgatttct ccgcgaagtg ctgttccggc tgtaataaca
    12601 actgctttgg cccggtagct cgctcctgtg gacgtcacta ctccatgaca aacaccatct
    12661 tcgacgatca gttcttcgac gattccttgt cttagcgtca aatttggttc tttttcgatc
    12721 gtatgtttca tttctgttga ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca
    12781 gctgggcctt ttcctgtgtt caacatacgc atttggatat aggttttatc gatattctta
    12841 cccatttctc cgcctaacgc atcgatttct cgaacgacaa cgcctttagc tggtccgcct
    12901 acagaaggat tacatggcat gaatgctacc atgtccaaat ttattgtcag taataatgtt
    12961 tttgatccca tacgtgcagc agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca
    13021 acgatcacgt catacgcatc tgcttcataa tgttccaatc gtttgttcct ccttttattt
    13081 tcctaaacaa aattgactga ataattgagt gattagctca tcttgtacac tgtcgccgac
    13141 aacttctcct agatagtccc agcaacgcgt catatctatt tgaacgagat ccacaggcat
    13201 tcctgaatca atacctgcaa tgacttcttc aagtgagagg gaagcttttt ctaacaacgc
    13261 gatatggcgg gtattagaaa cataagtagc gtctctttct cctgtttctc cgctgaagaa
    13321 taaatcagaa atagctgatt ccaaacgatc tagtccgtcg tttttagcta cggaaattga
    13381 aaaaactggt tcattttcga tcaatttctt caatttttct tgttccagct gtgctggcaa
    13441 atctgtttta tttagaagaa tgatccgttt caatccactt gtcgcttcca ataattgttc
    13501 atcttctgct gtcagtggtt cgctttggtt tagaacaagt aagatcaagt ctgcttctgc
    13561 taatgctttt cggcttcttt cgaccccgat ccgttccacc acatcttctg tttcccgaat
    13621 tccagctgta tcgattagct tgagcggtac cccccgaaca ttgacatact cttcgattac
    13681 atctcgagtc gttcctgcaa tatccgtaac gattgctttt tcttctctta acaaatggtt
    13741 taacaaactt gatttgccga catttggccg gccgatgatt gccgtactta acccttcccg
    13801 cagaaccttc ccttgcttgg atgtagcaag tagcgcttgg attctttgtt ggatcatctg
    13861 cgctttttca agcaataact tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc
    13921 aatatttact tctacttgtg ctaatgtctc taaaatctct tgtctcaatg agcggatcaa
    13981 agcagacaga ttgccgtcta actgattaag agctagtccc attgctttgt ctgtttttgc
    14041 ccgaatcaaa tccatcacag cttctgcctg tgaaaggtca acacgaccat tcaagaatgc
    14101 tcgttttgtg aattcgcctg gctcagctaa tcttgcacct tctcttaaca atagctgcaa
    14161 aatttgattg actacaacga tcccaccgtg acaattgatt tctactacat cttcccgcgt
    14221 aaatgtcttt ggtgctctca tcacggagac catgacttca tcgacaagct gattgctctt
    14281 gggatcaacg atatgtccat aatggatcgt atgacttggt acttctgata atcgtttgtt
    14341 tccagattga taaactttat ctgctagttg cacagcttga tcgccactta aacgaacgat
    14401 actgattgca ccttctccag gtggggtaga aattgccgca atcgtatcaa attctaaagt
    14461 aatttcagcc attttttcac ttcctttttc attttgcgca caaaaaaagt gcccactcca
    14521 ccctttatgc acaataaacg gtgaacttcg cactttgact gcttgttctt gattaaattc
    14581 tggtcataga ttatcacact tgctataagg aatcaagtgt ttaaaacaat tagatgaagt
    14641 tgagcctgtt ctgcttccat taccttatat ttaattgtcc ttggatgtct ctttgcgttc
    14701 tttggtttat tcgaagagaa gatactgtat aaacaaggct ttatctttct taaaaaaaac
    14761 ggtacactaa atggtagcgc tatctaaact atacactttt gaaaggagaa aatatgtgag
    14821 cccgttcaga tctgctgcat tggaagaaga aagtgaccct cggatcaacg atgtcgtttt
    14881 atctacaggg aatatcaacc agaaatatat cgtccgcgat atcgtttttg ttgtggaatg
    14941 cttagaagct gatttgtttg acccatcttt tcccaaagat gaccttttct taacaacgaa
    15001 gaggaaatta aagacgaagg tacttgaata tggtgcgaat gcagtaatca actgtcattt
    15061 tgagtatcag caaaaaaaac gcgacggaaa gtattttctg gaactttttg cttatggaac
    15121 agtgatccaa tttatccaaa caacgattgg ttaaatgaaa cagacaaaaa aagcggatca
    15181 aaactgaaga cagtttcgat ccgctttttt ctatcaaagt tagtgactac ttcatcgtat
    15241 actctgataa gaatgatttc tagtaaaata tacttggttt gaaaaacttt gtcaacgtga
    15301 tttattcaaa aagtaatgga atcaataata ttttttagca ggttctacta ctaaataacg
    15361 ataaggttcc tcaccttccg aatgtgtttt tacatgttca tctttgctta atgcagcatg
    15421 gatttgttta cgttcaaatg caggcattgg ttctaagaaa acaggctgcc ctgtccgttt
    15481 tactttttca gccgtgcgat cagctaaacg ctgaatgatc tcttggcgct tttcacggta
    15541 attgcctaca ttgactacta cagacagctt atttcttgct aaacgatgga tgaagacttg
    15601 tgccagatat tgcaatgcat tcagtgtttt tccatgtttc ccaatcagta tgccttgctt
    15661 ttgtgtttct aaatggaaaa caaccatatt atttgattct ctttccattt tgacaagagc
    15721 tggagcatca agctgttttg aaatttcagt aagataaact gccaattccg ttaaagctgc
    15781 ttcatcacct agctcactta cttcttctgt tttttcttca agaatcgttt cgttgactac
    15841 ctcgatctca gctgttggtt cttcttccaa aatttcttct acagtctgtt ctactgtttc
    15901 agaaactttt tcgctgatac ttggctggat cgaaagccgt gcattctttc gacccattcc
    15961 taggaaacct ttttttcctt cttccagtat ttcgatttcg acttggtctt tggccaaacc
    16021 caaatcagcc agacctagtt ggacagcttc gtctattgtt gcagcttcat aaatcggcat
    16081 tagcattacc tcccctatta tttccgtttt ttcttaggat tcattgcttt cttaagagca
    16141 cgttctcttt cgcggattct ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg
    16201 ttgttgatca gcagcgtttg tacaacttgg aaggcattgg aaactaccca gtataatgaa
    16261 agaccgctgg ctaaattgac acccatcact aaaatcatta caggcatcac gaaattcatg
    16321 atcttcattg atgcattcga ttcaatttga ctcatgctag ataaatacgt acttgcaaaa
    16381 gtaaagattg ctgctaaaat cggcaaaata aatgttggat ccgcgttccc taagttcaac
    16441 cataagaaat gcccttgttt caactctggt acacgtgaaa tagattgcca taacgccatc
    16501 aagattggca tttgtactaa taaaggaaga caaccagcat atggattgac gccattttct
    16561 gcatataaac gctgctgctc ttctcttaac ttactttgtg tttccggatc tcttgaacta
    16621 tattgttgct gcagtgcttt taatttcggt tgtaattctt gcgttttacg catgctcttt
    16681 gtttggaaat gcatcaacgg caataggatc acgcggatga tcagtgtaaa taaaatgatc
    16741 cctatccctg cattaccaaa tgacaacgcc ttgatcgcct cagcaaagta ataaacaata
    16801 taacgatccc aaattcctgt actctgtgcg ctgacttccc cagttccgca accggataag
    16861 acgatgacca aactaacgat accggccatc aaaagtaaac gtttgtattt cttcacttac
    16921 tcttttcctc tctgtcgatt atttttgcaa gttttaagac gtggactaaa ttggagcgaa
    16981 cctcatcata agtcaacccc ttcacacttg gtctagcaat cacaataaaa tccaactccg
    17041 gatcaatttc ttcttttata gaataaactg ctgctcggat ttttcgtttg acttcattgc
    17101 ggtttaccgc gttgccgacc ttttttccta cagataaacc aactcgaaaa tgaggctgtc
    17161 ccggtttttc caaacggtaa acgacgaatt tgcggttagc aaaagaatct cccgtatgga
    17221 acacggcttg aaactctcgt tcttttttta ctcgatacgc ttttctcatt agttttcttt
    17281 ccttttctat ctctcactta cgctcataat cataccataa ttacgattgt cctttcatca
    17341 tttccctaaa aaagagtata aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa
    17401 cttttcttcc tttgcgacga cggctagcta acacacgacg tccattttta gtactcatac
    17461 gtttacggaa tccgtgaact ttttgacgtt tacgtttatt tggttggtat gttcttttca
    17521 tatattccac ctccaaaatg ttcttatcta ttttataaac acagacatac tttgatagta
    17581 taacgacaaa gtagtgattt tgtcaatata aaatagaaga tcgattgtta aaacttatcc
    17641 acaagattat ttttttctat gggaatccta tgtggaaaaa gttatgcaca gggattatcc
    17701 acagataata aaaggctggt tttacacaga aaacacacgt gtaaaaaaag taatccacaa
    17761 tcaggcgatt tcctgtgtat tccgtctcct aactattggt atatctgtat tttttatcca
    17821 cagacgcaag aggaaatcta ttggttttta cgagttttcc acctgtgcat tagtggtgtg
    17881 gaaaactttt tttgacaact gtgtttttct cttttttctt tttgtcgatt gtgtggataa
    17941 ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa
    18001 ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat ccctcgatgc
    18061 tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta gctataatac
    18121 atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc
    18181 tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg
    18241 ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag aagcagagca
    18301 agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc taaaaaaagc
    18361 catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa accagatggc
    18421 tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc cgctcttctt
    18481 ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc atcaaatgct
    18541 gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg ctaatgattt
    18601 catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc gaaatgttga
    18661 tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt
    18721 tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa ccagtgatcg
    18781 gttgccaaac gagattccga aactgcaaga acgccttgtt tctcgctttg catgggggct
    18841 gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac gtaaaaaagc
    18901 ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag gacaaattga
    18961 ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa
    19021 tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa aatcaggaaa
    19081 aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt attatcacat
    19141 ccaattgaaa gatctcaaag gaaagaaacg cgtcaaatct attgttgttc ctagacaaat
    19201 cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg gagctgaatt
    19261 cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc agctgatgga
    19321 ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac taaattaatt
    19381 tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt caaaagttat
    19441 acacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag ttttccacag
    19501 aatcaacatg ccctattact tttattattc tttttaacta ataataaata aacaaacatt
    19561 caaaggagat acatcatgaa agttacttta aaccgagcta gctttatgca ggaattgcaa
    19621 actgttcaac gagctatttc aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc
    19681 acactgacac aagaaggttt gactttgacg gggagcaacg ctgatatatc aattgaaact
    19741 tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg ttccattgtt
    19801 ttacaagcac gtttctttag cgaaatcatt cggagacttc ctgaagaaac atttacttta
    19861 gaagttttag aaaataaaca agtagcgatc acttctggaa aagcgaattt tatcgtaaat
    19921 ggattagatg cagataacta tcctcatctt cctgttgtcg aaagccataa ccagatgaaa
    19981 ttacctgtac acgtattgac taaactaatc aacgaaacag tttttgctgt ctcacaacat
    20041 gagagtcgtc caatcttgac aggtgtgcat ttcattttat ctgataattc tttattagct
    20101 gtagctactg attctcaccg tctaagtcaa cgcgtgattc cagtagaaca agcggctgat
    20161 cattttgata ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc attaacaaat
    20221 gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa aacagaaacg
    20281 atgtatttct attcccgttt gctagaagga aactatccag ataccaatcg tttgattcct
    20341 tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc gatcgaacgg
    20401 gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat tcgtccagat
    20461 gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag cttgagttat
    20521 acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat gaaagcagcg
    20581 ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat tcgtccattt
    20641 acattggaac caacggaaga tggcgttcaa tttattcagc tgattactcc tgtacgtaca
    20701 aattaacaat gattttcaat gttcttgaaa atttaattga aaagaaatag aaaaaataaa
    20761 attttaaaca gaaggcccta aaatctcttt taaggctttc tgtttatttt tgtttaaatc
    20821 acctttttta gattaaatcg tcttatatgg aaaaatatat ggataaattt gttttattgc
    20881 gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg ggcattgaaa
    20941 caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa agaagtcaca
    21001 gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt gttagtcgat
    21061 ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt tgaagttcca
    21121 gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct
    21181 gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca agaacctcgt
    21241 catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt
    21301 agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct ggactgacga
    21361 tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat tagaaatctt
    21421 attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa agttaagtac
    21481 ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt tagtaaaagg
    21541 cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca atccaatcta
    21601 tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt acttgaagca
    21661 attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg aacaactagc
    21721 agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat tggagttttg
    21781 ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa tcgagtatct
    21841 caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac aatttctcgc
    21901 acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag ttggtcctca
    21961 tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg gatcgcaagg
    22021 acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga ttaaagaaga
    22081 gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg atgataatcg
    22141 tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa caacaacgac
    22201 acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg ttcaacaagg
    22261 gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa
    22321 gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg taaacgacct
    22381 ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg ggaaattgtc
    22441 gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt gattattgaa
    22501 aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga tatccaggca
    22561 aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg aggaaaattc
    22621 ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc tgttgtaaac
    22681 gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa
    22741 tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga tcgccatgga
    22801 acaacggttc actttacgcc agaccctgaa atttttacag agacaactga gtttgatttt
    22861 tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat gcgtatatcg
    22921 attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga cggcgggatc
    22981 aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga acctgtatat
    23041 ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac agacggctac
    23101 cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggtgg aacacatgaa
    23161 tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg
    23221 atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt gacagctgtt
    23281 atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa attgggcaat
    23341 tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa attcttgctg
    23401 gaaaacccaa gtgtgggccg gcaaattgtt gaaaaaggat tactagcttc cagagctcgt
    23461 ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga gattagtaat
    23521 ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga attgttcatc
    23581 gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc
    23641 atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga caagatcttg
    23701 gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg agaagatttt
    23761 gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga tgtcgacggc
    23821 gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc tattgttgaa
    23881 gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg gaagaatatt
    23941 acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt tgcttcactt
    24001 cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat ggatgatcat
    24061 caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt cagtgtggac
    24121 gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt agaaccccgt
    24181 cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta atcatgaagg
    24241 aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc taaccagcga
    24301 aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg cacttcctga
    24361 tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga acgaattagg
    24421 tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa
    24481 ataccatcct catggagaca ttgcgatcta tgagtcaatg gtacggatgg cacagccatt
    24541 cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg acggtgacgg
    24601 tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg
    24661 tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag aaaaagaacc
    24721 agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt
    24781 aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg ctatcgattt
    24841 gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac ctggaccaga
    24901 ttttccaaca ggtggtttgg ttatggggaa atcaggaatc cgaagagcgt atgaaacagg
    24961 taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa atggtaaaga
    25021 acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga tcgaacggat
    25081 ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg acgaatcttc
    25141 ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat ccgttatctt
    25201 gaataactta tataaattga catccttgca aaattcattt ggattcaata tgttggcgat
    25261 cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatc cttgaaaact atatcgaaca
    25321 tcaacgcgaa gtcatcacac gacggacggt ctttgaaaaa gaaaaagcag aagcaagagc
    25381 acacatctta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca ttgcgattat
    25441 ccgcgggtct aaatcagatg acgaggcaaa agcaacaatg atcgaacggt tcgatttatc
    25501 cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg gtttggaacg
    25561 ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt taactgacat
    25621 tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag acttgaatca
    25681 acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa gtctggaaga
    25741 tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg gttacatcaa
    25801 acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg tccaaggaat
    25861 gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc atgatacatt
    25921 gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata
    25981 cgggcgtaca gccaaaggaa tcccaatcat caatttacta ggtatcgatt cgagtgaaag
    26041 catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc tcttctttac
    26101 gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca tccgaagtaa
    26161 tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga
    26221 tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt tcaaagaaga
    26281 agcagtccgc gatatgggac gtacagcttc cggggtcaga gggatacgcc tgcgtgagga
    26341 agattatgtc gttggggcag ccttaatgaa ggaaaatcaa gaagtattag tgatcacaga
    26401 aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa
    26461 agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat tgaccactgt
    26521 ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc gtttcaacgt
    26581 atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga agatggaagc
    26641 tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac aaacagttga
    26701 agaacaagga tcagaagaat aaccatcaag actaagtgtg taagctagca agaataaccg
    26761 attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct cttaaaatat
    26821 tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatt agagcttgtg
    26881 agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc cgaaagtcca
    26941 cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt attcgtccaa
    27001 acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc ttgaaagata
    27061 acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga
    27121 acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca tcagcaaatg
    27181 ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt cacatgattg
    27241 ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg agaggagatg
    27301 actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc agatttgcgt
    27361 tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg taattttaca
    27421 aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg caaatcagca
    27481 gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg acgtatccaa
    27541 actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt agtagctgac
    27601 aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag cggcggattc
    27661 caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa tcaaacatct
    27721 caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt cggctcaagt
    27781 tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat aggagggaaa
    27841 tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact acattgctgc
    27901 taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat ttatttcaga
    27961 acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat
    28021 tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta gcgaagacta
    28081 ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc ctttttttct
    28141 gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt aaaatcaaat
    28201 tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc ccgaaacctc
    28261 tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta ccatttcgtt
    28321 ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat tggttgaaaa
    28381 tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg actgtagctg
    28441 aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct tatcagccat
    28501 caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat caaagtggat
    28561 taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag
    28621 tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt gttgtttatt
    28681 ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt cagccggtga
    28741 ttggtatttt atctattgat aactatgcag atgccattga taaattagat gacaaagaga
    28801 tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat gaataccatg
    28861 tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg atcgatctga
    28921 aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac
    28981 agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa
    29041 tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc gaccaagttg
    29101 tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa tctgctaaga
    29161 atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt cggatttttg
    29221 atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat gctttaggct
    29281 ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac atcgttatca
    29341 atgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat aagcatcctg
    29401 aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa gaagaggacc
    29461 tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc ctatatgagc
    29521 aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac
    29581 ctctgctgac ttatattgaa tcttcagctt cttcagcttc agagctagtt tctgagctga
    29641 tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca ttattgcttg
    29701 cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga acttttgacg
    29761 ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat ttattgagct
    29821 cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac tatgtgactt
    29881 ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt atagcagcaa
    29941 aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc attacaaagc
    30001 ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac gtccaaatca
    30061 tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa ctggaaaata
    30121 cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat atgaatcaaa
    30181 tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa aaggaaaagg
    30241 taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc taatcaaaaa
    30301 taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc
    30361 acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag aattcttgga
    30421 aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg
    30481 ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga
    30541 taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa acgtacctgt
    30601 taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag aataacaaat
    30661 cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa accgaaattt
    30721 atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc agattgattt
    30781 cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat aaaagagaac
    30841 tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc ttgtaaaatg
    30901 aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc gaatcccacc
    30961 gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg cagaaacgat
    31021 cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc atcaaattat
    31081 ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg ttactgtcaa
    31141 agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt atctctcaga
    31201 cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga
    31261 aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga aaggattcga
    31321 gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct tggaggtttc
    31381 ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact ccacgattga
    31441 aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa ctggctatca
    31501 aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc ttgctgcacg
    31561 tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag gaacaaaaac
    31621 agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg taaatcggat
    31681 gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc tttcggaaga
    31741 agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata tttttatcga
    31801 tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga
    31861 aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag gtaacggtaa
    31921 agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga
    31981 gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac agcgacaaga
    32041 caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag atgcggacat
    32101 cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg acgatgatgg
    32161 aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc gaagcggtgc
    32221 acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt cgctttcgcc
    32281 aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag ctctcgtaag
    32341 aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag atctccaaag
    32401 ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt tcaatcagct
    32461 gacccttggt attagctcag caaacgtcaa aatatgagga actattttga cgtttgtttc
    32521 tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg gtgttctcct
    32581 gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt ttttgaaatt
    32641 gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa
    32701 aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgaga
//
LOCUS       DACEDO010000027        32027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEDO010000027 DACEDO010000000
VERSION     DACEDO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.027"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..432
                     /locus_tag="IWO52_001321"
                     /pseudo
     CDS             1..432
                     /locus_tag="IWO52_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(558..1637)
                     /locus_tag="IWO52_001322"
     CDS             complement(558..1637)
                     /locus_tag="IWO52_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0355599.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1740..2180)
                     /locus_tag="IWO52_001323"
     CDS             complement(1740..2180)
                     /locus_tag="IWO52_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0355600.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2237..2647)
                     /locus_tag="IWO52_001324"
     CDS             complement(2237..2647)
                     /locus_tag="IWO52_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0355601.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2827..3261)
                     /locus_tag="IWO52_001325"
     CDS             complement(2827..3261)
                     /locus_tag="IWO52_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0355602.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3361..4209)
                     /locus_tag="IWO52_001326"
     CDS             complement(3361..4209)
                     /locus_tag="IWO52_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0355603.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4598..6364
                     /locus_tag="IWO52_001327"
     CDS             4598..6364
                     /locus_tag="IWO52_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0355604.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6466..7725
                     /locus_tag="IWO52_001328"
     CDS             6466..7725
                     /locus_tag="IWO52_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0355605.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7872..9173
                     /locus_tag="IWO52_001329"
     CDS             7872..9173
                     /locus_tag="IWO52_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0355606.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9176..10027
                     /locus_tag="IWO52_001330"
     CDS             9176..10027
                     /locus_tag="IWO52_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0355607.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10040..10855
                     /locus_tag="IWO52_001331"
     CDS             10040..10855
                     /locus_tag="IWO52_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0355608.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11006..12172
                     /locus_tag="IWO52_001332"
     CDS             11006..12172
                     /locus_tag="IWO52_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0355609.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12402..13049
                     /locus_tag="IWO52_001333"
     CDS             12402..13049
                     /locus_tag="IWO52_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0355610.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13133..14077
                     /locus_tag="IWO52_001334"
     CDS             13133..14077
                     /locus_tag="IWO52_001334"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0355611.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14212..15663
                     /locus_tag="IWO52_001335"
     CDS             14212..15663
                     /locus_tag="IWO52_001335"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0355612.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15684..16520
                     /locus_tag="IWO52_001336"
     CDS             15684..16520
                     /locus_tag="IWO52_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0355613.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16520..17179
                     /locus_tag="IWO52_001337"
     CDS             16520..17179
                     /locus_tag="IWO52_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0355614.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            17305..17994
                     /locus_tag="IWO52_001338"
     CDS             17305..17994
                     /locus_tag="IWO52_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355615.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18000..18956
                     /locus_tag="IWO52_001339"
     CDS             18000..18956
                     /locus_tag="IWO52_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0355616.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19000..19512
                     /locus_tag="IWO52_001340"
     CDS             19000..19512
                     /locus_tag="IWO52_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0355617.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19768..20316)
                     /locus_tag="IWO52_001341"
     CDS             complement(19768..20316)
                     /locus_tag="IWO52_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0355618.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(20405..21586)
                     /locus_tag="IWO52_001342"
     CDS             complement(20405..21586)
                     /locus_tag="IWO52_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0355619.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(21822..23330)
                     /locus_tag="IWO52_001343"
     CDS             complement(21822..23330)
                     /locus_tag="IWO52_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0355620.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            23489..23680
                     /locus_tag="IWO52_001344"
     CDS             23489..23680
                     /locus_tag="IWO52_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355621.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            23883..24800
                     /locus_tag="IWO52_001345"
     CDS             23883..24800
                     /locus_tag="IWO52_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0355622.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            24793..25866
                     /locus_tag="IWO52_001346"
     CDS             24793..25866
                     /locus_tag="IWO52_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355623.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26014..26532
                     /locus_tag="IWO52_001347"
     CDS             26014..26532
                     /locus_tag="IWO52_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355624.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            26513..26905
                     /locus_tag="IWO52_001348"
     CDS             26513..26905
                     /locus_tag="IWO52_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0355625.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            26981..27916
                     /locus_tag="IWO52_001349"
     CDS             26981..27916
                     /locus_tag="IWO52_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0355626.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28049..28828
                     /locus_tag="IWO52_001350"
     CDS             28049..28828
                     /locus_tag="IWO52_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0355627.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            28821..29138
                     /locus_tag="IWO52_001351"
     CDS             28821..29138
                     /locus_tag="IWO52_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0355628.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            29382..30992
                     /locus_tag="IWO52_001352"
     CDS             29382..30992
                     /locus_tag="IWO52_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0355629.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31149..31997
                     /locus_tag="IWO52_001353"
     CDS             31149..31997
                     /locus_tag="IWO52_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0355630.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        10536 a         5706 c         6261 g         9524 t
ORIGIN      
        1 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggt
       61 agatccagaa gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg
      121 aataccagaa aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa
      181 atcaaatcta gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt
      241 tgatagcgcc caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctgtcct
      301 gaaacgaaaa ataaacagct accaaaatcc ggagagtcac aaaacaaagt tatactgtgg
      361 agcggaatca ttctattaag tatagcaaca atgctttctg caaaaagatt taaacaaaac
      421 agatctctct aacatttgaa taatgtctgt gaacgcattt ttcaaataaa aagaaatatc
      481 cagaaaatga ccccaaaata ccagattgaa atctgatatt tggggtcgtt catactttat
      541 ctttcttcat atttatttta agggatatct gtttcttttg gaagatggtt cgggatcttt
      601 ttcactttca atacatctaa aaagaaggta aagatcggaa tgccgacaat caagccccaa
      661 acgccaaaga accgttcgct aacaagtaag accacaaatg tataaaaaat cggcagttct
      721 gttttgctgg acatcaactt cggattcagc acataagact caattagatg gacaatcaaa
      781 atcgtaatca ggatataaac aacatctcgg atgcctcctt gggaataagc tatgaaagat
      841 agaggaatac aagaaatgat aacacctgct actgggatca gacttaaaat gaaaatcatg
      901 atcgctaaac tcagcagctg atgaaagcca aatacagcca aggcgattgt agtgagcaac
      961 gtatttaaaa gagcgacaac aaattgagct tctaatacca aaccaaatgt attgacaaac
     1021 ttatcggcaa aatagtaaat atcttgaaag aaccaagaaa attcactttt taaaaacaat
     1081 cttgagaata caattgtttt tttcttttca atcataaaaa agaaacttaa gatgaatgac
     1141 atagcaaacg ccactgccat ttttcctata tcctgtaaat agccaagcgc aatcgaagcg
     1201 ccattttgaa gctgttcaaa aaaatcgttt cgctcaatat aagtatggat atattgtaaa
     1261 acaggatctg catcttttgg ctgatgctcg taaaaattaa taacggactg gatcatgtca
     1321 aatgtttgat gaataagcat cgggatatac tttgtgaatg ccagatagat aaggtaaacg
     1381 atcagtgtat aaatcatgat agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg
     1441 atttgaacaa gatgaatcat caaataagta aagataaatg tgagcaaaat ggttgtaatc
     1501 atacttctaa ctaagaaaag aacaaaaacg acaaataata aaacagtgag acggcgtatt
     1561 ttttcattcg ccaaaaaacg ctgataaacg gtcaaatcga caacctccaa ataacaaaaa
     1621 ataaacacgt tattcattat atgatggaat atgaaaataa gaaagagcaa aaagaaaaac
     1681 cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat ctagcagttt
     1741 cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag atgttgcagt
     1801 gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg aataccttct
     1861 gcttgttttt gcaacgcagc accttgagca gtcagcgtaa catttacgat tctttcatca
     1921 cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag taagggagtc
     1981 agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt ctttttcttt
     2041 tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg cttgaggagg
     2101 ggagtgtagt gtctaatgat ttctttagag gcggcatata aaggaaaaca tacttgatta
     2161 actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt atcatagcgg
     2221 atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata tttccagctg
     2281 tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc gtttcttcca
     2341 gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc gtttcacttt
     2401 ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt ttttgctgtt
     2461 tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac aattgttcag
     2521 ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg aatgtacgat
     2581 cgggattatg cacttctcca gagcgtccgc ctgtgttgat cattttcgta gaatacattt
     2641 ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac acaatataat
     2701 tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca tgaaacagac
     2761 gcgctttctc gaatagcaaa catatacaaa aaatatcaga gacaaaaaat aatctcatca
     2821 taaacattac atatatttct caaaaagttg ttttctttct tcaaaaatta cttcacgatg
     2881 gacctgatga agcatccata aataaccaaa aggatctctg aacattgagt tagtcacacc
     2941 aaagtccatc atttctgtca tttcttgaat ctctacacag ccatttcgta atgctttatt
     3001 atgtgtgtcc tcgatatcag agacgatgat attgaaccag atcgtatttg gatgttcagc
     3061 agtaggagca gtcaaaccaa gagacgtgtt ttcatccagc atatggaatc tcgtaccata
     3121 caaggtaaaa atgacttcat tattgcctct agggtaatct gttgcctcaa tcagttgtat
     3181 ttcatcaaag attgtttgat acagttctag tgctttcaaa ctatcattta atacaaaatc
     3241 gatttccgtt cctttatgca taaagatctc ctctcctttt cagtcgaaca gttactagat
     3301 tcttttatta taccagctaa agaaaacgca ttcaaaaaaa tagcggattt acttaccaac
     3361 tcattcgatt ggatgataag taaatccgcg tcccattact tctgctacgc tgctgacaat
     3421 gacaaaggca cttggatcga tttcttctat caatggtttc attcgggaaa attcatgatc
     3481 agtcacaacg atcaataaaa cctcttttga ctttcgatta tacccgcctt ttgcctctag
     3541 cagggtaagg ccacgtccaa tctcagcact taagcgaagg cggatgtctt ccaaataatg
     3601 ttcactcatg accataacag atttctttcg attgatacct gtttcgatat aagtcatgac
     3661 aattgaagtg attgcaatag ataaaatagc ataaagaaac tcttctattc caaaaataaa
     3721 cagattaaga gaaacaacga atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg
     3781 gaaatatttt ttaaaaatga ggggagggat cgtcgtaccg ccgctagaag agttgttttt
     3841 atataaaatc gcaactccta aagcaaaaat cgcacttcca aaaatgacag acaacaaccg
     3901 atctgaagta atcattgttt ctggaactat tgcaatgatt aaaggaaaaa ccaagcttcc
     3961 gaaaagtact ttaaaaaagg gttttttacc taaaaataga agagctagta ccaacataac
     4021 gatattcaac actaaaataa ctatagctcg gtcaaagcct aaagcagctt ccagtaatat
     4081 tcctagaccg ctgacacctc cagcagcaat atgatgaggc cctaaaaaca tattgatagc
     4141 gactcctaat atgaagaggg caaaaataat cttaatgtat gtccatataa attgtctcgt
     4201 ttctttcatt tattcacttc cttaattcat taataatact cttatcttat tataacttac
     4261 ttttcacctt aagttaactt taattaataa aaaaattagg ctagtctaaa ttattatgtt
     4321 tcattcttct agaatatact tctacgccaa aaactgttaa aaaactttta ctttagaaaa
     4381 ttggagaaat tgatcgctta agataaattc aggacactaa atagtacagg aagtagacta
     4441 aaacttttaa aagttttaag tctatctatt tggcaagttt aaaaaataaa aattcggtat
     4501 caaaaaactg ttaccgataa catttttgaa agcgttgtct tttgcttctc tatcacttat
     4561 agtgatgacg tcatattaaa acacaaggag gaaatcaatg gatacagctg caatttatca
     4621 tcgaccggaa agtgaatatg catttctata tacgaataca agatttcgta ttcggcttcg
     4681 tactaggaaa aatgatatca aaaaagtata tgtgctatgt ggagatccat ataccataac
     4741 aactgaaaaa tggtatcaaa aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca
     4801 tgattattgg gaaattgaag tgacgagcga aacaagtcgc ttacagtatg cgttccatgt
     4861 tgttggggaa gataacacgg actgttttta cggtgatcaa ggaatttttc cttatcgaga
     4921 aaatgtgatt actgaaccaa atttttattt tcgaatacct tattttcatc aaatcgatcg
     4981 attcactatt ccagaatggg taaaagaaac agtatggtat caaatttttc ccgaaagatt
     5041 tgcaaacggg gacaaagcaa atgaccccga acacacttta ccttggggaa gtaaagatcc
     5101 agaccgagaa gactttttcg ggggagattt gcaaggtgtg atggatcatt tggattactt
     5161 gacggacctt ggcatcaatg gcatctattt ttgtcccata tttaaagcca catccaatca
     5221 taaatacgat acgattgatt attatgaggt ggatcccgct tttggcgata aaaaactttt
     5281 aaaaaacttg attgatgaag cacataaaag agggatacgt atcatgttcg atgctgtatt
     5341 caatcatatg ggtgtccatt caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc
     5401 gatctataaa gactggttcc acatccgttt tttcccagta gattcttatc agatgactga
     5461 cttaccagaa actgctgaaa atatcccata tgatactttt gcttttactc cattcatgcc
     5521 gaaactaaat acagcaaatc cagaagtgca aaaatatttg ctggatatcg caacttattg
     5581 gataaaagaa ttcgatattg atggatggcg tcttgatgtt gctaatgaag tcgatcatca
     5641 tttttggaaa aaattcagag aagcagttac agaaatcaaa ccagacatct atatccttgg
     5701 agagatttgg cattcctctc aagcttggct gcaaggggat gaattccatg cagtgatgaa
     5761 ttatgcattt acagattcga ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca
     5821 aatggtcagt ggaatgaatc atcagcaaat gctttacaga gatcaggtga acgaaggaac
     5881 attcaactta ttagattctc atgatactgc caggatcttg accctttgcc aaggtaacaa
     5941 agaattgatg aagtctgtga tggcctttat gtttcttcaa aaagggtctc cttgtatcta
     6001 ttacgggaca gaaataggga tgactggtga agatgacccg gattgccgca aatgtatgat
     6061 atgggagaag gaggaacaag atctagagct attcggtttt ataaaagaac ttgtttctct
     6121 aaggaaacag ttgtctaaga ttatttctga aggttctact cagtggctaa tagtaaatga
     6181 tcgtgaagat aagctctatt tcacacggaa actagaggga cagatcatct atgtttactt
     6241 taatcagtca aaagaacctt gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa
     6301 ttgtcagttg ttagaagacg ggaaagcaga gatacgacaa tacggatttt tgatcatgtg
     6361 ttgaaaactg ttaccggtaa caacaagaaa gcgtttgcaa atgaagattt gtgcccatta
     6421 taataagggt gtaacagcac gttgaataca aaggaggaaa tgaagatgaa aaaaagaagt
     6481 gtgaaacagt tgggtttagg gttaatctct ttaggaatct taggcggatt agcggcttgt
     6541 ggcaatggta actcttcagc agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc
     6601 gttgattctg gatataaaga ttatataaac gaaataaaaa acactttcga aaaagaaaac
     6661 gatgtgaaaa tcaagctagt agaaaaggac atgttcgatc agttggaatc attagcgtta
     6721 gatgggcctg ctggaaaggg acctgacgtt atgatggcag catatgaccg gatcggtgcg
     6781 ttaggacagc aaggacatct ggcggaagta aaactgggaa atgaagcagc ttacgatgaa
     6841 acagacaaag cccaagtgac ttatgatgga aaaatctacg gagaacctgc agtaatcgag
     6901 acccttgttt tgtactataa caaagattta gtcgatacag caccatcaac gtttaaagat
     6961 ttggagaatc tatccaaaga cagtcgtttt gcttttgagt cagaagcagg aaaaaataca
     7021 ggatttttag ctaaatggac tgacttttat tattcctatg gtttaattgc aggttacggt
     7081 ggctatgtat ttggtgatga tggcacaaat ccttcagata tcggtttgaa taatgcaggt
     7141 gctgtagaag gaatctctta tgcaacagat tggttcaaaa atgtttggcc aaagggaatg
     7201 caagacataa aaagtgcagg agacttcgcc agccagcaat tcatgtccaa taaaactgca
     7261 gcaatcattg acggtccatg gcaagcgcaa acatacaaag aaaacaatat caattatggt
     7321 gtagccaaaa ttcctactct aaataatgga cagccttatc aaccgtttgg cggaggaaaa
     7381 ggatgggtag tcagcaatta tgctaaaaac aaagacctct ctcaaaaatg gcttgattat
     7441 gtaacgaatc aggaaaatca agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac
     7501 caacaagcta gggaaacagc aaaggctaaa aacgatgaat taacaacagc tgtgattgaa
     7561 caatatgaaa cagctcaagc gatgccaaac atcccagaaa tgggcgaagt ttggacaggt
     7621 gcagaaaact tgatgttcga tgcagcatca ggttcaaaaa caccaaaaga atccgcagat
     7681 gaagcagtaa aaacaatcag tgaagccata gaacaaaaat actgattaaa atgataaccg
     7741 tccattttta taaaaaatgg aacaggtgct tcagataaaa gtggcaagag cgagatggaa
     7801 aattatcttg ctcttgttgc cattatctta aaagcagttt gcggtttagc aagtttagag
     7861 ggggcattca gatgcagcaa gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt
     7921 gttcacttat tccagggatg gggcagtttg taaatggcca agcttttaaa ggtatcgttt
     7981 ttttcgccat attcgtccta ttcattggtc agatgcttct gggcggattt catgcattgg
     8041 taggattggt tactttggga agtataccaa tggaagatca ttcattattt attttgattg
     8101 aaggcacttt gcaattcatc attacaatca ttttcttgat tttttacggt ttgaatcttt
     8161 tagacgctta tcgagtagcg aagatgcgtc aagagggcaa aaaaatcaat cggacgatga
     8221 aggaagtcat acataatata ggtgatgaag gttttcctta tctattgaca cttcccgctt
     8281 atcttctcat gatttttacg atcattttcc cagtcctagt tacattgttc actgcattta
     8341 ctaattatga cttcaatcat attccaccag ccagtttaat tgattgggta ggggctaaaa
     8401 acttttttag tatcttcttt ttaagctcat atcgtaatac tttttttgca gtcttttcgt
     8461 ggaccgtgat ttggacttta tgtgcaacaa ctttgcaaat cacccttggt attctcacag
     8521 cggttgttgc tcaccagaaa tttattaaat tcaaacgtat tttcggtatc attttcttat
     8581 taccatgggc ggtcccagct tttattacta tcatgagttt ttcgaatata ttcaacgata
     8641 gcgtaggtgc catcaatact caagtgattc ctttcataaa ccagcttcct tttgtagata
     8701 tcagtgcaat ctcttggaaa acggacccga actggacgaa agtagcaatt atcttagtgc
     8761 agggctggtt aggatttccc tatatctatg tgatggttac aggaatctta caatcgattt
     8821 cagaagactt atatgaagca gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa
     8881 acatcacgct tcctgctgtc ttcctagtgg cagcgccaac atttattaca cagtatacgg
     8941 ggaatttcaa taatttctcc atgatctatc tgtttaacaa tggcggtccc ggaagcgtag
     9001 gcggaggagc tggttctact gatattctta tttcctggat ctataaactt acgactggaa
     9061 cttctccgca gtattctatg gcttcagcaa tcacgttgat tatctccgtc atcgttatct
     9121 gtgtgtcatt gctggtattt aaaaagacaa atgcgttcaa tatgggggat taacaatgaa
     9181 aaaatttcat tcgagtgctc gattccaaaa acgactttct cactttttta cttacttttt
     9241 tcttttaata ctgtcagtca ttatcatcta tccgcttttg atcacagcaa gtactgcctt
     9301 caagtcagga aatattgcag cttttagttt agatctttca agtgactgga ctttaggaaa
     9361 ctttactcgg ctattcagtg aaacacttta cgggacttgg tataaaaata cattgatcat
     9421 ttctgtccta acaatgatcg tacaagtgac tgtagtaact ttggcgggct atacttatag
     9481 tcgctatcgt tttatcggcc gcaaatcaag tttgatgttc tttttagtga ttcagatggt
     9541 accgactatg gcagcattga ctgcctttta tgtcatggct ttcttattag aagcgctcga
     9601 taagtactgg ttcttgacaa tgatctatat aggcggtgga attccaatga atacttggct
     9661 gatgaaagga tactttgaca ctgtaccaat agatttagat gaatctgcta aacttgatgg
     9721 ggcagggcat ttcaggattt ttttccagat tgtattaccg cttgtcaaac cgatgctcgc
     9781 cgttcaagca ttatgggctt ttatgggacc ttttggggat ttcatgttag ctaagttcct
     9841 gcttcgaaca cctgaaaatc agactgttgc tgtcggctta caaacattta tttctgatgt
     9901 gacaaaccaa agagtctctc tttttgctgc tggggcaatt ttgattgcac taccgatttg
     9961 tctattattc ttctttttac aaaagaactt tgttaccggc ctgacatctg gcggaacaaa
    10021 aggataggag cagagtatca tgcaaacatt tccgttaaac tatttttccg cattacgtac
    10081 gccaactcaa gtgtttgcgg gacgcaaact attatcatgg cctaaatttt tcttgatatt
    10141 tgtattttta gtttccttga tggtcatgcc tgtgactctt ttttatgcca atcagatcca
    10201 agcgattcca ttagaacaat ttctatccgt tcattcgctg atcgatgaac aaggaaccca
    10261 aaaattttct gaacttgaat tatctgaaac tgggttacaa gcatcgcaac aaacgatagc
    10321 tgtgacccct gaaattttgg taggtgtgtc tttatctgag aaacaacaat ccgatcatgg
    10381 aacttttatt gattttgaga aggaacaatg ggtgatccaa caaaaggata aatctggtat
    10441 acgccgctat acgatgaatt atagcccatc tttccaacca gattctgtac gtacgcccga
    10501 agatttccaa cgatttttag aaagagaatt ttatgccagt aatcgtccaa caatcatttt
    10561 atcttatagt ttaagtcttg gtcttgtcct atttgtcatg acgagtctca ttttattcgg
    10621 ggcttccttt ttcttgtgga tgacaagaaa aagccaactt tcatccattc atacattcaa
    10681 agagagtgca aatttgatgc tgaatgttat gggaataggt tccatggcag cagcagttgt
    10741 agggtttatc cactttgact ttatcttgat gctaagtgtc caaacaacga taactgtctt
    10801 gctgctttta tgggtttttg tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt
    10861 ttaagcgatt tatgcgacaa tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa
    10921 atttctgaaa aaattaaact tcttagttga ttcttttctt ttttagtatt aacgtaatag
    10981 ggtagttttt ttaaggagga aaatcatgtt agagaaaact ttatacaatc aattattttc
    11041 acgttctttc tcgctccctg tcgaagtaaa ttattgggac ggtacaacga aacaatacgg
    11101 agatacggac actcctcccc aaatcaaaat caatatccat gaagaaatcc caatgaaaga
    11161 gattacaagt aacgcttctt tggcactagg agaagcctat atggatcatc gaatcgagat
    11221 cgagggaagt atccaagcat tgatcaacga tgcttatcag caagcaaaca gctttttacg
    11281 aggaaaagat tatctaaaat ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa
    11341 agaagatatc catgcgcatt atgacttagg taacgacttt tataaacttt ggctagatcc
    11401 aactttgact tattcttgtg cttatttcaa tacacctgaa gatacgttgg aacaagcaca
    11461 aatcaataaa gtccatcata tcttagacaa actctttatc aaagaaggcg aaacgttgct
    11521 tgatatcggt tgtggctggg gaacattgat gttcactgct accaaagagt ataatgtcaa
    11581 ggcaactggg atcacactaa gtgaagaaca atacgatttg attaccaaaa gaataaaaga
    11641 agaacattta gaagataaat gccgtgtatt attaatggat taccgcgaac taaaaggcga
    11701 aacgtttgat catatcacaa gtgtaggtat gttcgaacat gtaggttctg aaaatcttga
    11761 aggttatttc aaagtggtaa aagatttatt gaaaccaaaa ggaacagctt tgatccatgg
    11821 aatcagccga caacaaggcg gtgcgacaaa tgcttggatc aataaatata ttttccctgg
    11881 cggttatgtc cctggtgtta ctgaattagt tgataatatt acaaaaaacg atttgcaact
    11941 gattgatttg gaaagcttac gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa
    12001 ttttcatcaa gtccaagatc aagtcacgaa agaaatgggt gattccttct atcgaatgtg
    12061 ggatctatat ttacaagctt gtgcagcttc atttgaatca agtaatatcg atgtgattca
    12121 atatttgctt gttcaacctg gaaacaatga catcccaatg catcgtgcat aattgaggca
    12181 aaagtactgt ttaaagacta tttaaaaaga agctaagttt tatgatgctc cagaagtgaa
    12241 cttttttgtc taacttctag gattgtctct aaagcttagt ttctttttct ttatattttc
    12301 ataataaaag tttggttctt taatttttaa acatgacaga aaattttttc ttcgttatac
    12361 ttgtaataga atactatttt ataacaaagg ggatcgaatc aatgaaaaaa actgttattt
    12421 atactagttt atttgcagct ttgtttttag ctgcttgtca atcaaatacc aatcaaggca
    12481 catctgggaa tacgtcttcc agtagcgaac agcgctcttc acaggtatcg gactcctcaa
    12541 caaaagcaag cacatcttcg tctagccaga caaaaagttc tagtgaagca agtactcagt
    12601 cagatatcaa agtgtctgca gctcgtgcga tcgaattgtt ccaagataaa tacccagata
    12661 ctgcaattac tagtttggaa ttagatagtg attggggatc ttacttctac aagatagaag
    12721 gtgtcgatga tcaaaaagaa tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga
    12781 cagagaatcc agaaactcta gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg
    12841 gtctggatgt tgataaattg atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag
    12901 taggaagcgg tacggcaaca gattgggatt tggataaaga tttaggtact acttattggg
    12961 aagtcaaagt aaaagacggc aatcaaagta catcagtcaa gatcaatagc caaacaggcg
    13021 aagtactgtc aactgaacaa gatgattaag aatttcttcg gaaaacaaga gttgattttt
    13081 cttttagtca tgatacacta gacaaaataa cttgtttgaa ggagcgattt acttgagttt
    13141 agaaacgacc tttgccaatc atatctttgc caatcctttg atgaatgcat ctggtgtcca
    13201 ctgcatgact acccaggaat tagatgaact ggctcattca gaagcaggag catttatcac
    13261 taagagctgt acgatcaatg aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc
    13321 tttagggagt atcaattcta tgggacttcc aaacttaggt ttttcttatt atctagagta
    13381 tgctcttgcg tatgaaaaag tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc
    13441 cgggatgagt gtccaggaaa acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa
    13501 tgggatcact gaattaaatt tatcttgtcc caatgtgcct ggaaaaccac aactagctta
    13561 cgattttgag gccacttatg aaacactgaa agaagtattc agcatctttt ctaagccgct
    13621 aggaatcaaa ttgcctcctt attttgattt tgctcatttt gatcagatgg ctgatatctt
    13681 gaatcagttt ccattgacct atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat
    13741 cgatacagaa caagaagcag tagtcatcaa accaaaagaa gggttcggcg gcattggcgg
    13801 agagtatatc aagccgactg ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc
    13861 agaaatccaa atcattggta caggtgggat tcggacagga caagatgcat ttgaacatct
    13921 gctatgtggc gcaagcatgt tacagattgg taccgagctt cataaagaag ggccggaaat
    13981 tttttctaga atcataaaag aattaacgca gattatgtca gaaaaaggat atacgtcaat
    14041 tgacgaattt aaaggcaaat tacgcaccat ttcatagcaa agagacgaag gtcatgattg
    14101 atcttcgtct ttttttataa aaaacactcg tctgttcatt cctgtgcaag cgtactcatt
    14161 gtatgttaga cgaacttgtg ggaaaataac agaggcaaaa aggaggataa tatgaatgat
    14221 gcaacagaat tagcaaaaga gcttaaagaa aaagaattta gttttcaaga actagttacg
    14281 gatatacaga aaaaaataaa gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa
    14341 ccattcgaga gtaattccca cgagctattg attccccagt cagaaaaagg gccattggct
    14401 ggtatcccat ttcctttaaa aatgctcggt caagaaaaaa agggatggct agctacttca
    14461 ggatctcgtt tatttgaaac acaccgagct tctcatacat ctaattatgt gcggcaagca
    14521 gaagcgatag gtctagttcc ttttggacaa accaatgcgc cagagtttgg tttcaaaaat
    14581 attacagacc cagtcatcta tggcccagca agaaaccctt ggaaccttga atgtacaccg
    14641 ggcggatcaa gtggaggagc tgctgctgca gtttcatcag gaatcgttcc tttagctggt
    14701 gctagtgatg gcggtggttc tattagaata cccgcttcgt tttgcggatt gattgggctg
    14761 aaaccttcgc gagggacgat gccagttggt ccatatgctt ggagaggatg gcaaggagct
    14821 gctattgatt tcggcttgac agtttctatg agagacacgg aggctttatt ttatgggatg
    14881 agaagtatcc attccggtgc gccatatcaa gtatcgccag ttgagtggca aacacatcca
    14941 agaaaaaaac ggttgaagat agctttttgt gttgattcac cgatccactc acagattagt
    15001 gaagaggcag tacttgctgt aaaaaaagct gtagatttcc ttggaaaaga agggcatgat
    15061 ttgacagaga ttccttaccc actagatggt cgtaaactga tcacgtctta ttatcagatg
    15121 aatggcgcag aaacagcagc aatgatccgc tcgattgaaa caggcatcca gcgagctgtc
    15181 agaaaagaag aacttgaacc catttcttgg actttgttcc aatatggtca gaagctctct
    15241 gccgctactt acatcgattc tctgcatgta tgggatgaag cagcaattgt catggaaaac
    15301 ctacatcaag aatacgatct attcctttca ccaacgactg catttaccgc ccctaaaatc
    15361 agcgaagatt tgcaaagtga tgcgataaga gcacagatgg aacacatcag tgattgttct
    15421 gaagtagaag gtttagacgt tgtttcttcc atgtttgagg agagcttgaa gatcacacct
    15481 tatacacagt tggctaatct gactggacaa ccagcaatca gcttgcctac tcatttgaca
    15541 aaagaaaaca tgcctctagg aatccaattt atggctgcca gaggaagaga agatttgcta
    15601 tttcaaatcg gctatctatt tgaagcaaat catcagttcc atttaccaaa tccatggaaa
    15661 tagcagaaac aaggagaaga agaatgaaga aaaaaagatt gtttgctcta ataactagtg
    15721 ccttttttat tttaagcgct tgcggtacag ctgccgctaa tcaggaagat aacagctgga
    15781 gtaatgtaga aaaaaagaaa gaattagtag tagcgacttc tggcacactg tttcccgcat
    15841 cttattataa tgatcaaaat gaattagttg gttatgatgt agatttggca aaagaggtgg
    15901 ctaaacggtt aaatgtcaaa atctccttta aagaatacaa cgtggatgga caaatcactt
    15961 ctgtttcaaa aagagaatca gattttgctg cgaacgattt tggtatcaca aaagaaagag
    16021 aaaaaaaatt ctccctttca gagactttca aatattcatt tgacagtatg atcgtacgaa
    16081 aaaaagatga ttcaggaatc cactcacttg aagatcttaa agggaaaaaa gcagctggcg
    16141 aacccaatac tagctatatg cgtttagcgg aaaaacacgg agctaaatta gtcacatatg
    16201 acaatgccac gaatgatcag tatttaagcg atgtagcaaa tggtcgaaca gatgttattc
    16261 taaatgatta ttatctgcag aaaatggccg tatctgcttt accagatatc cctgtaaaaa
    16321 tactggagaa cgtttatttc aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg
    16381 atgctctgaa aaagaaaatc gatggcgtat taaaagagat gaaagaagat gggacgacaa
    16441 agaaaatatc tgaaaaatat tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg
    16501 ctgagtcggt gagtgagtga tgtactttcc tctaattgat ttttccctca tgtggacttc
    16561 acttcctttc gtactgcaag gcttggtata cacattaggg attggcttcg tgtcgtttct
    16621 tttaggaaat ctggtagggt tgcttttgac agttttaggt cttttggatt ggctgcctct
    16681 gaatgtattt attcgttttt atttatcgtt ctttagaggc attcccgcac tagtcctgct
    16741 gtttctgtta tattttgggt taccttatca attaagtgcc ttaacggctt cagtcatctg
    16801 ctttacgatt accagcagtg catttattgg tgaaatctat cgaggatcac tagcaggcgt
    16861 ttcttcaggt cagtgggacg ctggtttagc ttctggtttt tctttctttg gggtattgaa
    16921 atatatcgtt ttgccgcaag cttttcgaat ctcagtaccg gcactcagta acgtagctat
    16981 ggatctagtg aaaggtactt cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa
    17041 agcaaagatc ataggcggtc ggacattcga ttatttttct ctctatattt tagttgcact
    17101 gatttattgg gtcatttgta tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc
    17161 tatgcatcag tcaatctgaa aaatagaaat ggtgctagcg tggctggcac catttctatt
    17221 ttttgtttct tgtatattct gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg
    17281 aaaatatgtt cggaggtaga aaacatgaag accccgctaa ccatcgaaat agaatcctcg
    17341 ttataccaat attgcatcga gcaaggcggc ttggttgtag aagaagtcac gatgcctgat
    17401 gaccaaggaa ttgtagatac gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg
    17461 cgatgctatg agctgaaagt gacaaaagca gattttcgct ctacagccaa actgtcattt
    17521 gttggacatt ataattattt tgttttacca agtaaactat atgaagaagt aaaaactgaa
    17581 atccctacag gaataggtgt tttagtgtat cgtccgtaca cggttgaaga ggagatgcct
    17641 gcctctggta catttctgat tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa
    17701 gaagctttga caaaccgctt catggcttct ttattcaggg aagtaagaaa agcaaagcaa
    17761 atggagcgag gcgtaaaata ttttccaaca gatcaattgt acaaagaatt gaaaagacgt
    17821 tcagaaaatc aagatccatt ttctaacgaa cgatattatg aacggttttt agaggaaata
    17881 gctagtcagg caatcaatga gttgcaagaa gaagttatcg cgcttcagca tgattatgaa
    17941 tatttgaggc aacaacagca gatccgtcgg ttgcctacag agccattgga gtgaaaaata
    18001 tgtactatcc ttatttaaga ggaaaacaat ttgacctatt agcattaaaa gaagcactgt
    18061 cgcgaggatt gctctccaat aaaatccaac cagtgatcga accagtacgt gattccgcta
    18121 cgcttaaaaa tgtgatcgaa ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa
    18181 atccccaagt aggacaattc aaattattcg atcagcatgt gcacagttgg acagtcaaag
    18241 aaaatagctc tgtcgtccct gcacagatct taacgccaga aaatctcagt gaagtactgg
    18301 attcacctcc agcgtttctt gttttcgatg gacagcacta cccaaaagat acagaggtgt
    18361 ggaaacagtt ggcaggagtt gattcgaaat ttttgatacc agatactagt cgctttcgca
    18421 tctggctgcc cgaaaacaaa attgtgatcc gtgactcttt tcaaacgaga aagcatgttg
    18481 aaagttatgc agataaaaca gatgactttt ttagtgatga ctatcttttt ttccacgcag
    18541 acggatatat tggattttct gatttcacga tcgaaggtag ccgttatttc gataaagggt
    18601 tccctagccg agcgcttgct atccatctaa cgtatattga tgcttacggc aatatacgcg
    18661 tgaaacattt tgtctctgac agtaacgact ctgctaaaga ccaagcattg aagtttcttg
    18721 aagcaggaga taaaatgaaa aaatggatca tgcgtcacca tcatcaactc ttgattactt
    18781 caggaatgat tgaattattg gctctttacc agcaacaaaa attccctgga ctgggagtgt
    18841 tgaaaaagtg gtcacttatg catcatttgg aacttattag tcacctgcta gatcatccaa
    18901 aagattggct taaatattat tcaaaagttc aggaactata tgaagtgatc caatgattag
    18961 ataatgtaaa tgaaaaagga atagaaaaga ggaaactgga tgtatattcg gcaaacaagg
    19021 aaagaagaag tagaagaagt ggtagaaatc attaatgccg cagcggattt cattgcttcg
    19081 caaggctctc ctcaatggca aaacgggcag aaaccaacaa aagaaacgat tttgaacgat
    19141 attgaaagaa aagaaagcta tgtattaata gtggaagatc aaattgctgg aacagctgca
    19201 ttagtctcaa gcgtggatcc cgtttacaca aaaatagatg gtagttggaa agaatcaact
    19261 ggtccttatt tatcgattca tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta
    19321 gcaaaaccgt tcttagaaca tttgttgacg attgcagcca gcaaaaatat ccaagatatc
    19381 cgtatcgata cttatccagc taatgaaccg atggaaaaga ccatctattc tgctggattc
    19441 acttatcaag ggatgattga atttcctttt gaacacggcg aaagaaaagc atatcaaata
    19501 atcatcaatt gactataaaa tcatctgagc aaagataaag acattaacac tactgaaaaa
    19561 aacaatcata tgatagccaa gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa
    19621 gaatttccga aaatgcttct caaatttttg tggaatttta gcgtatttta cgaaatagct
    19681 gctttctgaa actcccttca tcgagaggtc gtgacatagc ttttgtcacg acctctcggc
    19741 ttatcctacg agtattattc gatacattta tatcaataat ccttttttta ttttatctgc
    19801 agcttcttgg aacgctccat cgtttgacac gactaaaagt gaggggatag ctttcaaacg
    19861 tttcaactgt gctaaatctt ctacataaac cgccagtctg gctgaaatct gttcagatga
    19921 atctccacgt tttttcaggc gcgattgcaa tgtttcttta gagatcgtaa taaagatagg
    19981 acggactacc gtcccgaaat actcatataa atgccaaaaa ccatttgctg tcaaaacggc
    20041 gtaacaattt cctactgtca gtttcttttt aatttctgct ttactggaac catatttatg
    20101 ttgagcatat tggtcccact ctgcaaactc atttcccttg atcatctgat tgaatgtatt
    20161 gtcagaaaca aaataataat cctcttgatc tttttcccca ggtcttggtg atcgattcgt
    20221 gtaagtaatg atcttttgtt ccggctgaaa aatagcttct gcgagtgaag tttttccaga
    20281 accagaagga ccgataaata catagcaata attcattgtc atttctcctt tgatgattga
    20341 acaagtgaaa aagctttgga aaatatttcc aaagctttta aaagtaactt tgcttttttg
    20401 tatattatac cagcaagcca gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta
    20461 gcaaaatttt caatccgtgt tttgcgacaa gatttccttt ttcttcattt aatcccttca
    20521 tcgcaccaga aatgatcccg atagaagaga agttggcaaa tgaaacgagg aagacagata
    20581 taattgctgt tgtacgctca gtaaaatgga aattaccatt tgctaattct gtcatagcaa
    20641 caaattcatt tgtaaccagt ttagtcgcca tgatactacc ggcatccact gcttctttcc
    20701 atgggattcc gctgatgaag gcaagagggg caaagatgta acctaggatc tcttggaaag
    20761 tgatgccgaa tatcccgtgg aagatagcat tgatcatggc aatcaaagca acaaaaccga
    20821 taagcatagc agcaaccgtg atcgccacat ggaaaccatc aagaatgtat tctcccaaca
    20881 tttggaaaaa tgtctgttct ttgttttctt caatgatcag ctcatcttct ttttcttcta
    20941 atacataggg atttacaatt gaagccagaa tataaccgcc aaataagttc aaaacaaggg
    21001 cagtgatcac atatttagaa tcgatcagtg ccatataaga tccgacgatc gacatagaaa
    21061 ctgtagacat agccgataca ctcatggtga acaaacgttt ctcagatagg aagggtaatt
    21121 ccttttttat ggaaataaaa acttccgatt gtcctaaaat agcagaagct actccattat
    21181 aagattccaa tttgcccatc ccgttgattt tacttaatcc tagaccaaca gctttcataa
    21241 ataatggaag aatcttgata tagcgtagaa taccgataat agcagaaata aatacaattg
    21301 gcatcaatac ggaaagaaag aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa
    21361 agtcaacacc ttgcccagca aaagcaagaa gatgatcaaa catttttgcc aatacagaaa
    21421 caaccgctgt accaaaagtt gtacgtaata atacgaaacc aagaacaagc tgtaaaacga
    21481 acatcacaat tagtggtttc aatttgattg cttttttgtt actgctgatc aaaaaagcaa
    21541 gccctaatgt gacaaataaa ccaataatac ctaataaata actcaaataa tcactcctat
    21601 tccaaattcc gttttatttt acctgttttg caaataaaat actaatcttt agtgaaattt
    21661 tcattacttt tttagagtga aacgcattca ttttccatta gaaaataaag cgaacaaaat
    21721 aaaaggttta aaaagtgtaa cgtcttagta aagtctataa aaaaacgaaa ctatcgatta
    21781 agctaatagt ttcgttttca acatttataa gtaaactttt tctattggta atatagattt
    21841 tcggaagggt agacaggtgc gcaggtaata tcgattccta gttttttcaa tgtttgttca
    21901 tcatttttat tgaccatcac agtagagtgg gcttgtgctc cctcaagttc agctagttta
    21961 tcataagcaa cttgagcgat tggattcgtt actgcactga ttgataaagc gatcaagact
    22021 tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt
    22081 gtttcaagaa ctaatggtga caataaagga atgtcatctg cgatatgggc taaatgtttt
    22141 actgcattta agataactgc cgctgacgca tccatcaatg aacttgtacg cccggtaatg
    22201 atgacttcat ctggtaattc gattgcaatc acagcagaag gatcttttgt aggactttgc
    22261 gcttgaatct tatctgcata gtcatgggca gggactacca cgtttcggtc ttcaggacgt
    22321 aaaccgactt cttccataat cagtttgatt cgattaactg cttcttcgtc gatcaatcct
    22381 tttttgaagt cgcattctgt ttggaaataa cgacggatga tttcttgttt agaggcttct
    22441 tggacaacgg tatcatccac gatcccaaat cctacacgat tcacacccat gtcagtagga
    22501 gattgataaa cagattcttt accagtaatt ttttcaataa tacgtttgat tacaggaaat
    22561 gtctcaacat cccgattata gttcacagct actttattgt aggcatcaaa gtggaaggaa
    22621 tcgatcatat tgacatcttt tagatcgact gtggctgctt cataagcaat gttcagcgga
    22681 tgtttcaacg gaacattcca tactgggaaa gtttcgaact tggagtaacc agctgcatgt
    22741 cctttttggc tttcatgata cagttggttc aaggaagtag cgagctttcc acttcctggg
    22801 ccaggagctg tcactacaac gattggttta gttgtttcaa tatactcatt ttttccaaaa
    22861 ccatcttctg agacgatttt ttctacgttg actggatagt cttcgatttc tgagtgttta
    22921 tagaccttga tgtttcgtct ttctagttta ttgataaaga ctttcgtggc aggctgtcca
    22981 ctatagcgag tgataacgac actgttgatt aataagccat aaccacgtaa ttcatcgatt
    23041 aaccgcaaga tatccatatc ataagtaatc ccataatcac cacggatctt attacgttca
    23101 atatctccag catagacgca aatcagtatt tcagcttgat ctttcaattt ttgcagcaat
    23161 ttgattttcg catcttcttc aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact
    23221 agcttcccac caaattctaa atataatttg tcatagtggt tcactcgttc caaaatatac
    23281 gctgactgct cttcaatata tttttctgaa tcaaaaccaa tttttttcac taggttccct
    23341 ccttaagtac taaagcattt tttcattttt ttgaggattc gtcaactgga agagcgaaat
    23401 tcctcaaaaa aacaaagaaa atggtttaaa ccaacttgtc tagatagtta ttcattggta
    23461 tgatagtttt tgataaggaa ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata
    23521 taaacactat atccgttcga tttttaccgg ttatgaaaga atgcttgaag atcccgaatt
    23581 ggaacaattg acgtttactt atctaaacga agaaacgcag ctaactcgtg atgagcatca
    23641 gcgcattcat ttcacaacaa gagatctgcc tagtaaataa aaaataagat aaaggtcatc
    23701 ggattcgatt ctgtcctatc agatggaaag actagttgat cgaaaaaaag gctgtaccaa
    23761 attttggtgc agcctgtttt atggagcatt gcccgctctt ttttataaca attttttaca
    23821 gaaaatggta ctattgaagt attaataagt gaaaaacaga tgagtaagag gaggcaagta
    23881 gtatgaaaga gccagataca aatgcaaatt atgagccaga cgaactagag gagacgaatg
    23941 cggatcagga tacaggacaa gaatcgtctt ctttttacga tatcaaagaa aaagcaagca
    24001 tgaatctttc ccaattatta gaagacttac aggcttttga aaaagctgtc cgcgaagaag
    24061 atatccctga gatctaccgt ttatacaatg gccgattgaa tgaagaatta aaaagaagct
    24121 caaatgaaaa tcacgagatc gatcaattgc tgatgcgtaa gatccatgat cgttttttac
    24181 aggaatttcc ttttatggaa caagtagaaa acatctcgcc aacgatgagc tattacaaaa
    24241 tcggcactta ttaccatgat cggcccacgg tcggtattga tgcaagtctg ccagaaattt
    24301 ttgttttgcc taagatcgat gaagagtggg agaaatattc ccaaccggag tcaacggact
    24361 atgacaaaaa aatcaatgag ctagacgcaa aggtgatcac tgctcagaca gaaattgaac
    24421 gtttagatga acaaataaaa gaaatcaatc gacaaatgac tgatttgaac gacaataaag
    24481 gattcttgaa tcgtaaaaaa atcgaggaag aaattcaaga attagagaag aagaagcaag
    24541 tactatctaa tgaaaaatta ggctggcttc cttatattga gacgcctgaa acaatccagc
    24601 agcaaaaaga agcattgaag caagaagcaa gagcagacca gcttcgtgca gccatcgtag
    24661 aaaaagaaca aaggcaaatc agacgctatt tcggtagcaa agagggattc ggtcaagcaa
    24721 tccatgaatt cttgatgaat tatcttggca atgagaatcc taataatcaa tctaatgaag
    24781 gaggttccga gtatgaatga acacgatgaa gcagaatgga caaaaaatga agaacaaaca
    24841 gaagatcatg aagaaacgaa cacttcattg gaagaacaaa caagtaatcc tctggatgac
    24901 acagaagaaa tccctatgct agaggaaaca aaaatacacg aaaaagaaaa agtaactccg
    24961 acgaatacga atactgcaga aaacacccat gcttacgaaa taactgaaaa tcccgggtat
    25021 ttagaaggaa aagagcaaga tcatcaagat atcgtcagtt tgattgaaac agcggaagca
    25081 gaattagcgg atttacgtat gagaaaagat ttgatcgaat cagattttcc acaattatta
    25141 gaatcatatg atcgttttat attgaatgaa gaaccaatct ccacgatcgg aaagacaagc
    25201 ctgatcgaat acttcacgta tgaattactg aaaccgttga atgatattgg tctggaacgg
    25261 tcaaattcag cttatgacac atggaaaaca agacattttt cgatcaatta tacgttgaat
    25321 ggacaaaacg aattagtctt ttcctttgta ttaccgacca tcgatcaacg ataccaagtg
    25381 gaatcgatga gtctcctaaa cgtatcacca gagacaatgg aaatcaatgt ccaagatgac
    25441 cgggtgcttt ctttgatccg acactggtca gtggatcgag tattctctgc agggcagatc
    25501 acgatattca atcacaaatt gaatcaatta ttagcccatg caagaacact tggttttacc
    25561 gttaatcaga cattactaga caatacgaag ccacttcgtt tgaatcttca gagcgaattt
    25621 gaattgacga atgaagtatt ggatgatatt tttatcgtgg caatgaaaaa tccatcctac
    25681 gatatggaaa agatcaacga aactacttat caagtacgat tagataaagg acagtcttta
    25741 acgataacaa caaacgaaaa agatcaaaca gtcttgtcga tctcatctgg agactaccca
    25801 cgatctgtca ttgatttctt tatcaattac gaattcttgg tcccattaat ggtgagaaag
    25861 tcctgattgg ataagttgga ttaccagatg tcattcagat aattctgttc caaaggactt
    25921 gcggcagtta tccagaataa ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa
    25981 taacttgctg atgaaggagg gatttcatta ggaatgtcca caaataaaat agcacagctt
    26041 agcttattgt ctgctgcttg tgtagcaggt aggatcgctt ttcaatttat tccgaatttc
    26101 cagccaatga ctgctatttt tttatttata gtactttact taagcttaaa agatgcgtta
    26161 attgtcatga gtctttctat tgcgatcagc agtttttatt ttggtgttgg accatgggtc
    26221 atcggccaat ggatcagcta tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga
    26281 aaaacagtgc aaaggaatct atggttaaag ggagtctgtt tttttctggc gggcatggtt
    26341 tacggaatag gtatgaccgt ttttgatacg ctgttatatc gtttgcctca accttggatc
    26401 tattaccttc aaggggtgtc gtttgatttg atgcacagta taggcaatgt tgtgttcttt
    26461 cttgtttttc tgccagtcgt caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa
    26521 aataatttat agtttgcttg caatgagtgt cagtatactc attttgacaa gttgcagtac
    26581 aacctataca caatcggctt ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac
    26641 aatcacgtta caagaaaatg gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg
    26701 cgatgattta ctgagtattt tgaaacgaaa ttttgaagtc aaagaagata acggcttcat
    26761 cacttcgctt gaagggcact cacaggatga gaaaaatcaa ctctattgga tgtttactgt
    26821 ggacgggaaa agtgctacta ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt
    26881 cattttcaat ttgtctaaac tataagtttc ataaaaaact gttaaagggc gaatattgcc
    26941 ataattcagt gagttcagtt gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag
    27001 cggaatgttt tccgtaattg ctgctctagg ggctaatatt ctagttgcta tcagcaaatt
    27061 tgtcggctat gcaatcagtg gcagtgccgc catgctaaac gaaagcatcc acagtatcgt
    27121 tgattgcagc aatcagattt tgctgttatt cggtgataaa caagccaata aaggacagag
    27181 cgagttgcat caatttggtg aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat
    27241 gatgcttttc ttcggcggtg gggcacttgg ggtgatggaa gcttttgaaa agttgacgca
    27301 tccatcccat gaagtgggta atccgatcat tgttatagca atactgttat ttggtttggt
    27361 aatcgaagga agttcgttac gcgtggcaat gaaagaaatc aaagaattga atactgaaaa
    27421 actttccacg atgcgctttt tgcgcgaaag tcgacacagt gaaatactga ttatttttac
    27481 ggaagacttg tgtgctgtaa tcggtttggt cttggcttta gcaggaacaa tattgactta
    27541 tgcaaccggg attgctgcat ttgatgcgat cagcggattg ctgatcggtt tcttgctgat
    27601 gggagccgcc attttcctag caaaagaatt ttatagtctg atcatcggtg aaagcgtgac
    27661 agcaagcgat ttatcaaaaa tcaagttagc ttttgaacgt cctgagatca gtcggctgat
    27721 cgatataaaa acagttcatt taagtccaac ggagatttta gttgcagcaa aagcagatat
    27781 cgttggtaac aaagagaatg aagcttactc ggtcatcaat gatattgaaa aaaatatgcg
    27841 tcagtctctt cctgacaaga agatgtatat atacattgaa acagataaat atgacccaaa
    27901 ttattctcgt aaataaaacg tttattcatg aattgaagca aaaatgagaa aaatataatc
    27961 ttgtttttaa ataaaacggc tacaatttag cgaatttatg ttacaatgag tccgctgttt
    28021 atttgttgta attaaaggag atattttaat gaacaaaaag aaaagtgcgc catggcttca
    28081 agctttgcat gcagtgatgc cactttgtat aagctatata ccagtaggat tagcttgcgg
    28141 agtattgttg caaaaagttg gatttgatcc tctcttatca ggactgttat cgattctggt
    28201 cttttccggt ggcgcgcagt ttttagttgc atctatgctt acgacgcaag catcatttgc
    28261 aacgacatta ttgatggttt tctttttaga attacgctat acattattag gagcaagtct
    28321 tgccggtttt atgaaaaaag aaaagcggac gtttttggct gtcttttctc aatcactgaa
    28381 tgatgaaaac tatgcggtga actacttaaa gttttcaact gacccttcct ggaataaaag
    28441 aaaggcttta tatgtcaatt ggttctcaat gactgcatgg gccggtagca atatgatcgg
    28501 taattttttc ggctcggtga ttcgagtaga tgctgacctt gtccattttg ctttgacggc
    28561 catgttcatt ttcatgttta tcatgcagat gaaaagcgca ctactgatct ttacaggttt
    28621 attctcaggc gtattagggg tcgtattcat ggtattattc caaaatactt tcggattgat
    28681 tgcagcaaca gtcattgctt catttgccgg atttatgctg gagaaggcat tcaaaaaaga
    28741 aaaagctaag aaactaagag accgcggtaa agatgtcaat gagccgctct tcaacttccc
    28801 ggaacaggag gccaatcacc atgactaaac tatatttgct acttttagtt gcttgtctgt
    28861 tcatcgtggc atatatcccg cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc
    28921 catcctggtt cagtgactgg atgaaatatg taccggtggc attatttgcg gcgttagctt
    28981 ttaaagatgt tttcattact catgaacatc tggatattgc ttggaatatc aaaatagctg
    29041 caatgattct agtagcaggc gtggcttaca aaacacgttc gatggcgttg tcagttttga
    29101 caggacttgc gtctgtcttt ttgctgtcta tgctttaaac tgttttattt tattaaaaac
    29161 agagattgcg acataagttg ttcaaactga tctatccgaa ctgcatttgc aacagtcaat
    29221 tctggatgca agtttattcg tagggataaa aaagattagt gttggcttat gatgcaagct
    29281 cttttttatt ttaggaatta gagagggatt tgtccttcta tgattttttg ctataatagg
    29341 gaagatgttt attttatgaa agaatagttg agggaattta aatgaataaa aaattgatgc
    29401 aaggaacatt ttggctaacc tttgctaatc ttctatgtaa agttttaggg gtagtctatt
    29461 tgattccctg gctaagtatg atggggaaca atcaagatgg tatgcttgct acgacattgt
    29521 ataatgttgg ctatcttcct tatggattgt tcctgatgct tggtacagtc ggatttccaa
    29581 atgcgattgc aaaaaaagta gctgtagcca caaaagaagg agataacgct gcttgtcggc
    29641 tgatttttag aagtacgatc aatatcatgt ttgtcatcgg tatcgcctct gctgcactga
    29701 tgtatttatt tgctccttta ctagccggaa tcagtccgat tgctaacgtt aataatggga
    29761 tactagctat cagaagttta tgcccatctt tgattgctat cccaattctt agtgcaatga
    29821 gaggatattt ccaaggaaaa aatgatctgc ggccttatgg aacttctttg attatcgaac
    29881 aggctgtgcg agtgatcgtt atcttggctg gtacttatta cttgcgcgta ttgactgacg
    29941 gaaccatatt agaagctgta ttgatcagta cagtcgcttc tttcttcggt gggttagctg
    30001 cgattatcca tatgtatatc gtcggacgca gaaaagactt cttcgaactt aaagattttt
    30061 tgatttccag ccgttatttc gaaagagaaa accgatctgc ctctgtctct atcattcggg
    30121 aaacactacc gtttattttt gtcggctcag tcattactat cgttcaaatg atcgaccaag
    30181 taacgatgaa accactgctt cactttttcc gtccagagat cgcagatcag caactggaat
    30241 tcttattcag ccgtgcttct gtcaatccaa ataaattgac attgatcctg atctccatgg
    30301 taggaacagt agctataagc agcttgccaa tattaagcac gatgaagaaa aaagatcgga
    30361 tacaaatcga aaaaacagta ggagatagtt tttcgattgc gttattgatt cttcttcctt
    30421 cattgactgg aatgtcttta ttagctggac cactatacac attgtttttt ggctatgatc
    30481 ctgaaagtgt cggatatttc caaatggctt tgctagcttc tttgtttttc tctttattca
    30541 cgatcctatc tacgatgctt caatctttga atcaccatcg ttttgcgatc cgattaactg
    30601 tagaagcaat tatcctgaaa gtcatcttcc aagtaatcgg attaggatta gttggcggat
    30661 acgggatgag cttatcaaat ggcttagcat tcggtattgt ctttgttcgc ggctatcact
    30721 ttatgtgcaa agaataccga atagcacctt tagcaaagat cagcaatttt ttcttaaaaa
    30781 ctttccgctc caccctgatt atgcttgcag tatgttttgc cgttttcttt ttgctgaatc
    30841 agcgattgtc aatggaatca aaaagctatg caatgattta ttgtgtggct gtaggaagta
    30901 ttggcggctt agtattttta ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa
    30961 atgttaaaca tcatagcaga agcaaagaat aggttaacta atcgtttttt gctgtcactt
    31021 gaaaaacggc aactcgtatg attacgcttg tcgtttttct attttccaat tttagtttca
    31081 tatctaatct tacttatgat atagttataa ggtctggaaa aattctttga aacgaggaaa
    31141 ttaaacacat gaaacttgca gtagttacag atagttcggc atatctgccg gaacgtatca
    31201 agaaccatcc cgacttattc gttattccaa tccctttgat tatggatggg aaaatttata
    31261 acgaaggaat cgatatcgaa gcggatgaat attatggact tttaaaaaac agtaaagatt
    31321 ttcctactac ttcacaacct gtcgtagggg aagttttggg tttatatgag gaattgaaag
    31381 agaaagggta tgacacgatt ttaagtatcc atctttcttc agggatttct ggctttgtca
    31441 acacattatt tgcgatgaaa gatgacatca gcggagtaac ggtcattcct tatgactcta
    31501 agatcactag tatgccgatg ggacacatgg tagaagctgc cttagactta aatgatgaag
    31561 gtcagtccct tgatgaaata cttgcacata tcgatcgaat ccgagataat acctatgctt
    31621 atctgattgt agatgatttg aacaatttag tacgaggagg acgtttgaca aatggtgcag
    31681 cgctgattgg cagcttatta aaaatcaaac cgatcctgac attttctgaa gggaaaattg
    31741 ttttattcga aaaaatccgt tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg
    31801 gtagaagaaa taacgaaata caaccgccag tcaaacttta cgtgatacat gcaaataatt
    31861 tagaagtagc gattgcggag aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg
    31921 aaattggtca tttcggacca gtcattggta cacatttagg tgaaaaagcc atcggcattg
    31981 ccatttcagc gcaataattc cttttattca aaaaacatac caaaaat
//
LOCUS       DACEDO010000028        31500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEDO010000028 DACEDO010000000
VERSION     DACEDO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.028"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(76..543)
                     /locus_tag="IWO52_001354"
     CDS             complement(76..543)
                     /locus_tag="IWO52_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0355631.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="IWO52_001355"
     CDS             complement(663..2201)
                     /locus_tag="IWO52_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0355632.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="IWO52_001356"
     CDS             2281..2967
                     /locus_tag="IWO52_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0355633.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IWO52_001357"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IWO52_001357"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0355634.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IWO52_001358"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IWO52_001358"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0355635.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="IWO52_001359"
     CDS             complement(4453..5583)
                     /locus_tag="IWO52_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0355636.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="IWO52_001360"
     CDS             complement(5583..7766)
                     /locus_tag="IWO52_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0355637.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="IWO52_001361"
     CDS             complement(7763..8788)
                     /locus_tag="IWO52_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0355638.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="IWO52_001362"
     CDS             complement(8833..9660)
                     /locus_tag="IWO52_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0355639.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="IWO52_001363"
     CDS             complement(9657..10724)
                     /locus_tag="IWO52_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0355640.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="IWO52_001364"
     CDS             complement(10750..11586)
                     /locus_tag="IWO52_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0355641.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="IWO52_001365"
     CDS             complement(11583..12452)
                     /locus_tag="IWO52_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0355642.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="IWO52_001366"
     CDS             complement(12522..13805)
                     /locus_tag="IWO52_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0355643.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="IWO52_001367"
     CDS             complement(13819..15504)
                     /locus_tag="IWO52_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0355644.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="IWO52_001368"
     CDS             15779..16804
                     /locus_tag="IWO52_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0355645.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IWO52_001369"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IWO52_001369"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0355646.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IWO52_001370"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IWO52_001370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0355647.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="IWO52_001371"
     CDS             25476..26444
                     /locus_tag="IWO52_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0355648.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="IWO52_001372"
     CDS             26462..27367
                     /locus_tag="IWO52_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355649.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="IWO52_001373"
     CDS             27364..28170
                     /locus_tag="IWO52_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0355650.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28327..29295
                     /locus_tag="IWO52_001374"
     CDS             28327..29295
                     /locus_tag="IWO52_001374"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0355651.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29612..30136)
                     /locus_tag="IWO52_001375"
     CDS             complement(29612..30136)
                     /locus_tag="IWO52_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0355652.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30423..>31500
                     /gene="scm"
                     /locus_tag="IWO52_001376"
     CDS             30423..>31500
                     /gene="scm"
                     /locus_tag="IWO52_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0355653.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSD"
BASE COUNT         8837 a         6803 c         5932 g         9928 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctccct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggtaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgggaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg taaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttgcttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    28201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    28261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    28321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    28381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    28441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    28501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    28561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    28621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    28681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    28741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    28801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    28861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    28921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    28981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    29041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    29101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    29161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    29221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    29281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    29341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    29401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    29461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    29521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    29581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    29641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    29701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    29761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    29821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    29881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    29941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    30001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    30061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    30121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    30181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    30241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    30301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    30361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    30421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    30481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    30541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    30601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    30661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    30721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    30781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    30841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    30901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    30961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    31021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    31081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    31141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    31201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    31261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    31321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    31381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    31441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca
//
LOCUS       DACEDO010000029        30871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEDO010000029 DACEDO010000000
VERSION     DACEDO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.029"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(402..956)
                     /locus_tag="IWO52_001377"
     CDS             complement(402..956)
                     /locus_tag="IWO52_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0355654.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1082..3011)
                     /locus_tag="IWO52_001378"
                     /pseudo
     CDS             complement(1082..3011)
                     /locus_tag="IWO52_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3317..4033)
                     /locus_tag="IWO52_001379"
     CDS             complement(3317..4033)
                     /locus_tag="IWO52_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0355655.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4034..5857)
                     /gene="glpO"
                     /locus_tag="IWO52_001380"
     CDS             complement(4034..5857)
                     /gene="glpO"
                     /locus_tag="IWO52_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0355656.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5871..7367)
                     /gene="glpK"
                     /locus_tag="IWO52_001381"
     CDS             complement(5871..7367)
                     /gene="glpK"
                     /locus_tag="IWO52_001381"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0355657.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7503..8963)
                     /locus_tag="IWO52_001382"
     CDS             complement(7503..8963)
                     /locus_tag="IWO52_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0355658.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8953..10248)
                     /locus_tag="IWO52_001383"
     CDS             complement(8953..10248)
                     /locus_tag="IWO52_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0355659.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10580..11257)
                     /locus_tag="IWO52_001384"
     CDS             complement(10580..11257)
                     /locus_tag="IWO52_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0355660.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11287..12378)
                     /locus_tag="IWO52_001385"
     CDS             complement(11287..12378)
                     /locus_tag="IWO52_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0355661.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12479..12913)
                     /locus_tag="IWO52_001386"
     CDS             complement(12479..12913)
                     /locus_tag="IWO52_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0355662.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13497..14954)
                     /gene="ascB"
                     /locus_tag="IWO52_001387"
     CDS             complement(13497..14954)
                     /gene="ascB"
                     /locus_tag="IWO52_001387"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0355663.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14951..16846)
                     /locus_tag="IWO52_001388"
     CDS             complement(14951..16846)
                     /locus_tag="IWO52_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0355664.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16904..18679)
                     /locus_tag="IWO52_001389"
     CDS             complement(16904..18679)
                     /locus_tag="IWO52_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0355665.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18729..20225)
                     /locus_tag="IWO52_001390"
     CDS             complement(18729..20225)
                     /locus_tag="IWO52_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0355666.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20419..22041)
                     /locus_tag="IWO52_001391"
     CDS             complement(20419..22041)
                     /locus_tag="IWO52_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0355667.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22369..23682)
                     /locus_tag="IWO52_001392"
     CDS             complement(22369..23682)
                     /locus_tag="IWO52_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0355668.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23927..24151)
                     /locus_tag="IWO52_001393"
     CDS             complement(23927..24151)
                     /locus_tag="IWO52_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355669.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24171..24539)
                     /locus_tag="IWO52_001394"
     CDS             complement(24171..24539)
                     /locus_tag="IWO52_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0355670.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24735..25445)
                     /locus_tag="IWO52_001395"
     CDS             complement(24735..25445)
                     /locus_tag="IWO52_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355671.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25463..25960)
                     /locus_tag="IWO52_001396"
     CDS             complement(25463..25960)
                     /locus_tag="IWO52_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0355672.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25997..26449)
                     /locus_tag="IWO52_001397"
     CDS             complement(25997..26449)
                     /locus_tag="IWO52_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0355673.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26584..27189)
                     /locus_tag="IWO52_001398"
     CDS             complement(26584..27189)
                     /locus_tag="IWO52_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0355674.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27639..28259)
                     /locus_tag="IWO52_001399"
     CDS             complement(27639..28259)
                     /locus_tag="IWO52_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0355675.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28261..29130)
                     /locus_tag="IWO52_001400"
     CDS             complement(28261..29130)
                     /locus_tag="IWO52_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0355676.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29153..29560)
                     /locus_tag="IWO52_001401"
     CDS             complement(29153..29560)
                     /locus_tag="IWO52_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0355677.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29579..30868)
                     /locus_tag="IWO52_001402"
     CDS             complement(29579..30868)
                     /locus_tag="IWO52_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0355678.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8763 a         6691 c         5104 g        10313 t
ORIGIN      
        1 ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg
       61 atttctacat ttttgcataa atttggctta ataccgagaa actggctttt gaataccgtt
      121 taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa
      181 tagctgctca tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg
      241 tttaccaaga ggttgtgagc ctaccattta actccgaatc acaagaaata atttcaaaaa
      301 caacttctcc tgtttttggt tcggttggct tattatcgag aagtagctac tgggagtcta
      361 tttatcaaaa aaaggaatgc gacataggtc acaccccttt tttattttct ttgaaacatc
      421 tttcggtact cgacagcata caagacaaaa gattgtaaaa gaccgtaagt gatgacggca
      481 aaaatgactc tcacccacag cgttggtaat tctgggaaaa gcagcaaaag gatcatgcca
      541 acatagatga cagcagtcgc tactttgcca taccacttcg ctccacttaa tttgatattt
      601 ttagatagcc cgattaatcc agctacaaac ataaagcctt cttttatcaa gaatagacca
      661 aacaacagcc aaagccacgg tcgataccat gccatagaca aaatcagaaa aagttgtgtc
      721 aatttatctg caaacggatc aagcaccttc cctaagtctg ttacctgatt atatctccga
      781 gcaatgaccc cgtctagata atcagacaaa cctgagacaa caatgaccgc tcctgctata
      841 taaaatgact gtctcagcac aaaataaaga taaatcggca gcaaaaaaat acggaatatc
      901 gataaaagat tcggaatcgt ttgtatttct ttacgccagt ctgtgcttaa tttcatttta
      961 tcgccctacc tcctcagcac tcttttcttt ttattttagc ttacacgggg aataaaacca
     1021 atgatttcac tgtttttatt tttctttaca gacactgata ttatgagcta atttgagata
     1081 atcacttgct gttttcatcg tttactcttc tttcttcctg tcgtttgcgc atttctttta
     1141 ctagtacttt atgagctttt tttgatcctc gctctaggtc acgctgccgt ttacgtgttt
     1201 tataatcgat aaacagactg tctaaatcgt aacctgtcgg gtacagatct tctgctgaac
     1261 gcaccagacg aacacgctga cgaggcacgt ggattatttt tttgtctaaa tatacatcaa
     1321 tcgtatcttc acctatatct tcataaacta acgcttcttt ttggtattca ttgacaaata
     1381 tccgatcacc ttttgcaaag agctgacgtt cttttttatt ctccttccta tttgtaactg
     1441 ctgtcttagg aagaaattct tttttctgtg tccgataggc tttttccgcc aagtaacgct
     1501 ctgcttgatg gatcaatttc atcgacatat ccattttatg agcaatccac aaagcttggc
     1561 tttctcctgt ttcttccaac ccgcaactga tatttaggac gaagtgcttc ccgatcgaaa
     1621 gccatagcag ccggcacaaa atcttcatga tctccggcga atttcttgat ttctccataa
     1681 tgggtcgtgg caatgatcaa tgccccttgc tcatacatag attccatgat tgcgatcgct
     1741 agtgcagccc cttcatttgg ctctgttcca ctgccaatct catctaaaag taccaacgta
     1801 ttccttttta tctttttcaa aatagcagcg atgttttgca tatgaccaga aaatgtactc
     1861 aacgcatttt ctaaattttg gtggtcaccg acatcgacga aaatctcatc aaagacagca
     1921 agctctgtcc cttcttttgc cggaacttgc ataccgaaca tcgccatcaa agtcaataaa
     1981 ccgactgtct tcaacacgac tgttttgcct ccggcatttg ctccggtgat aatcaagcct
     2041 cgatagtctt taccgatttc aacatccaat ggtaccgcat gctcaggtaa aaatggatgt
     2101 ctgccttgct tgataatgat atgttccgat ttattgattc taggtgtgct tccattgatt
     2161 tctcggctaa attttgcccg agcaaagatg atatctaaca ccgtgatcgt ttcgatcaat
     2221 aaatcgatca cttcttcgtt ttctgcgata gcaccagtca atgccgccag gacttgatat
     2281 tcttcactga tttcttcagc ttttaacaac tgatagtgtt cattcaattt agaaatcaca
     2341 gttggttcaa taaaaaccgt cgttctttta tttgattcat cgataatcgt tccatctatt
     2401 ttatttttat agctggcttt gataggaatc gtatagtatt cgcctttttg aacaatcatt
     2461 gcttcttgga tcatttcttt gtttttcgga tgacgcaaga atttcagtaa ttttgactgg
     2521 atttcttttt cgattagttg taattgtttg cgtactttac gcaaattacg tgacgcgtcg
     2581 tcactgactt tattattttt tattttctgg tcaatcaact cttctacatt tatcaaatca
     2641 ggaagatgtt tgctataagc aaacaacaag ggtgtctggt attgattctt atcaaaaaac
     2701 tttgtgatca tccgactgct tctaagaaaa tccgcatatt cgatcaaatc ggttggctgc
     2761 agaatcaacc cttttttgac ttgttctgtc agtgcatcga tcctcggcaa ccccataaat
     2821 ggaacatgct gactgctttc taagatcaag cgtgcttctc ttgtttcttc ttgacgatcc
     2881 aagacagttt gtaggtttgt cgagaccgtc atatcgctga ttcgtttttt gctatagttt
     2941 ccaattgctc ttgcttggat ttcctttttg atccgatcaa attgtaattg actaatcatt
     3001 tcgttgtcca tattctctcc ttttcttgaa gagatacctg tcccaaaata aataagggcc
     3061 tagagataaa taaaaggata gaacacagct taaaaaagtc atgtcctatc cttattagaa
     3121 tacaaagata ttgttttcct gacaggtacc gtttcataag cacaccaaat aaactttgtt
     3181 tattggctat tcgacttact gattagatac cagctacact cacaaaacat accccattct
     3241 tctaggtctt atttattttc cacttaacta tagcaagaca cattttggtt gtcaaacttt
     3301 agtatgtttt atagatttac accattaatg aatagacacc taccgcgata attgctccga
     3361 tcattggacc tgcgattggt acccatgaat atgcccaatc tgaagttcct ttattagcta
     3421 ttggcaatac cgcatgtgct aatcgaggac caaagtcccg agcagggttg attgcatatc
     3481 ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa tccacctaca gcgaatacgt
     3541 tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa cccaagaact aatacaaatg
     3601 taccaattaa ttctgtgatc acatttgctg gataattccg tatagcagga cctgttgcaa
     3661 acgttcctaa aatcgcgctt tcatctttcg ttgcattcca atgtggcaaa taagaaagcc
     3721 aaacaagaat ggcccctgca aaagctccta aaacttgcgc aatgatgaaa ggaacgacaa
     3781 gattccaact aattgcccct gtcatcgcca tagctagaga gactgcagga ttcaaatgag
     3841 ctgggcccat gaatccagaa atatagacag ccattgttac agcgagtccc caaccaaatg
     3901 cgatgacaat ccagccagaa gcctgtgctt tacttttgtt taaattcaca gccgcacaca
     3961 caccatctcc aagcaaaact aggatcagtg tcccaataaa ctcccccatg atttgagtca
     4021 tatcactgtt catttatttc tcccccttta gttcccgcag atctgattct gcaatcaatg
     4081 cttctaaacg tttagtctgt tgcgccttct cctcttctgt ccattcaaaa tatccagcaa
     4141 ttgcatcgat cacaggttgc ttgatctcat caagctgatc tcgttcaaac aacagatgat
     4201 ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat ttcttcttct aaaccataac
     4261 gtaaccgagc tgactcagcg agactcaaac caggataagg tgccatttct tttgctaatt
     4321 caaagatacg tcgggcatta gtcccgtaaa aatcagcgat ataagtagca tcttcttcct
     4381 ctaaaccggc tgctactcct tcttttgcta attctgtcac tgtttcttct aatttagttg
     4441 gatcgaagtt tccacctgaa atctgatatt ttttagagtc gatttctttc gtctctattc
     4501 cgtattcttc ttttaataat tggcgtatca atcgcaaagc tccttctgcc atcttacggt
     4561 aatcggtgat tttcccacca gataaagtca gcaaaccatc tggttctctt tctaaagaac
     4621 tacctctgga aatcgtagaa ggtgcttttt catcacggct gttttccaaa tggttcaaca
     4681 cgtcttctac ttcagcacga gaaactttgt tttccttata ttcacttact gtatcgacaa
     4741 ctttattgaa gctcttgtct gaaatcgatc cattatctcc accattgtaa tcagaaccag
     4801 aattaccaat caacagtgga cgaagtcctg cccagcttgc ttcgatatca ttcaatgtga
     4861 tatttgcttc tggatagcga tgattgatca catccaatag ataatctaca tcttcttgtg
     4921 tgactttcgg atccgtaaaa tctccttggt aatccgtatc tgtcgtacca aagtaagtct
     4981 tgttttctct tggaatagca aaaaccatcc gcccatcttg ctttcctgta tcgaagtatg
     5041 tgggttgcgg tacaggcagt tttttcgcat ctacaactaa atggatccct ttagttggac
     5101 gcattttagg agagactgga cgcgtaaaat tcaagtttcg tactttatct acccaaggac
     5161 cactcgtatt gatcactaat tttgctttga tttcgatcac ttcatctgtc agcaggtcac
     5221 gagctttgac gccaacgatt tgatcgccat catataaaaa gccaaccgct ttcattttac
     5281 ttactagata agccccatct tcggcagcct ttttgatatt atcaatcact aaacgggcat
     5341 cattgttgcg gaaatctaga tagacacccg cgccttttag cccttctttt ttcaaaaacg
     5401 gttctctttc caatacttct tctggtgtca gcgtataatt ctcatatttg gttcctgtca
     5461 catttgccag tttgtcataa aggtccattg ctactttgac cgagaacata ttgaaggttg
     5521 ttgctccttc gccctcatag attggcaaaa gcattggatc tggttttggg atgtgtgggg
     5581 caatactttg tacgactgca cgttcaccaa ctgtgtcagc tactacttcc acatcaaatg
     5641 tcttcagata acgaataccg ccatgaacaa gtttggtcga gcgagaggaa gtcccttctg
     5701 caaaatcctg catttcgatc aatcctgttt tgattcctga tgctgctgac tggactgcca
     5761 caccggcacc agtgattccg ccaccgatga tcaacagatc caattcttct tgctgcattt
     5821 tttgaatgct atcttgtctt gtcttgtttg aaaacatatg gatttcctcc ttattcaaat
     5881 gcttgggtag cttttactgc ttttttccaa ccgttgtaaa gtttttctcg gcgctcttcc
     5941 tccatttgaa cttcaaatag cttgccagct tcataaaatt cgcggatctc gtctgtatct
     6001 ttccaataac cgacagctaa tcctgctaaa aatgctgccc ctagcgccgt tgtttccaag
     6061 ttttctgctc gttggatcgg tacattcaag atatctgttt ggaattgcat cagatattca
     6121 ttgtttgctg caccgccatc tactttcaat acaggaatct tgattccagt gtcatcttgc
     6181 atcgtatcga tgatatctct cacttgataa gcaatcgact gtaaagtagc tttaatgata
     6241 tcttcttttg tcgtaccacg tgtcaagcca aacatagccc ctcgtgctgc ctgatcccaa
     6301 taaggagcac ctagcccaac gaatgcagga acaacataaa cctcatcttc gcttgtagag
     6361 cgtttcgctg cgtcttctga atcgctagct ttttgcaaca tttgaagtcc atctctcagc
     6421 cattgaatcg aagaaccagc cacaaaaata cttccttcta aagcatagta aacctttcca
     6481 tttatgccgt aaccaattgt cgtcaataaa ttatttttgg ataattgcgg tttttcaccg
     6541 gtattcatga caataaatga acctgtacca taagtattct tgaccatgcc tggttcgaat
     6601 gccatttgtc cgaataatgc ggcttgctgg tctcctgcca tccctgaaat tggtgcttcg
     6661 cttccataaa aatgataacc ttgcgtataa ccataaactt ctgagttaga agtgactttt
     6721 ggtaacatca ctcgaggaat atttagtaaa tctaaaatct cctgatccca atctagctga
     6781 tggatattga acaacatcgt ccgactggca ttcgagtaat cagtaacatg tgcttgtcca
     6841 tcggtcagtt tccatactaa ccaagaatcg atcgttccaa acatcaattc gcctttttca
     6901 gctcgttctt gcgcgccttc tacatgatca agaatccagc gtatcttcgt tgctgagaaa
     6961 taggcatcga tcactaaccc tgttttttcg tggaaaaatt cttgatagcc ttcatttttt
     7021 aagttatctg caattccagc tgactgtctg gattgccaaa caactgcatg atagattgga
     7081 cgtcctgtat ctttttccca tattactgtt gtttcacgct ggttcgttat gccaatccca
     7141 gcaatctgat ttggtttgat ccctgattcg atgaacgctc cggcaataac tgactgtaca
     7201 gaattccaga tttcatttgc atcatgttcc acccaacttt cttgcgggaa atattgcgtg
     7261 aactcttttt gagaactgcc gatgtgtctt cctttcttgt caaagataat tgctcgtgag
     7321 ctagttgtac cttggtcaat cgccatgata tacgttgatt ctgtcattgt gattccctcc
     7381 attatatgca aacgctttct ttaactactc ttatcatact actgttttaa aaaaagaaac
     7441 tgtcaaaatt ttattctttt tttctttttt tgacagtttt ttattatgaa gctatttttt
     7501 aatcaattct tttctttttt cagcgtctct attcttcttt tcaatcgaat aatgtcgtaa
     7561 ggtgacgcaa attctgacag ataatattca cactgttttt tgtttctctg gcaaaatgtg
     7621 atcaccaaat catattcttt accttcttca taacttccaa tctcaatacc gccgattccc
     7681 ctcagttctc tgatcagata ttgttgaaaa gcaatacgat aaatcggcag actttctaaa
     7741 tcgattccaa tcactactgg ttttgcaata taaaaatcga tcatcaaaag aacattggca
     7801 tactggatca tcaggtaact aagctcacta tcttcagctc ttttcctcgg caattgctgc
     7861 accgctgttt cctgtaattt cgccagtaac tgtgttaagg aatgcccgag cattttctgc
     7921 tgctgttcca aaagccgatc acgctcatag acttcgatct ttccaacaaa gaaaaaccat
     7981 tcattgttga tctgggaaat ttggtagcct actgcctttt cttctttgat tttcaaacga
     8041 cgaggacgat aatgccataa catcgtttcc cgaatgtaag tatctaaaac agcggttggt
     8101 aactttttgc tccgtgtcaa atcatactga taaaatattt cttcgtctac tatcgaaaaa
     8161 gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat tcagttctgc tgctgttcga
     8221 ctaagatgca tcacgatgat tggatcgatt gcttgataaa gccggtccga ctgatagagt
     8281 aatttctttt ctttcaaagt tgcgtacgta cttttttcat tcaatagtct ttttttcgta
     8341 atcccgagcc agcaagcaat ctgtgcttct gctgatgccg taaacgttgt ttctaataca
     8401 cggtccaatc ctagaatgaa cggtcttttt tctggtgtat tttgcaaaaa aagcggtcgc
     8461 tgatcttccg ggatatattg gaatagttca taataaaaat atcgtatctg tagctcttct
     8521 ccgtataatt gaccgttttt gatttgtaat ccaaattcct ctaagagctg gttcaattct
     8581 ttgatctttc gaaaaacaga agactcactg atattaaacg ctgtcgctaa tcgaacgatg
     8641 gatacttgct gatgttctaa taaatagacg agcatcttaa acttcaagct ttcctgcaat
     8701 aaaaacaata gaattttttc caaacttaca tcttcgtcca acacaagcag cgcctgatac
     8761 tcatttcgct gtacttctac ttttttttcc atcattcggc caatcaattc aatatcttcc
     8821 aaatattgat caaacgaggc ttttgacagt tggacatgct ctctcaaacc attgatagag
     8881 gctgttcccc cttgcagaac aaggtttcgc aaaatcagga tctgtaactg ctcttttctt
     8941 tctaataatg cttcaatctt cataggcagt attcgccacc tttgaccaca ccatatctgg
     9001 cacacggtat tctggctgga aaaatagctc atgttcttct aactgttcaa acgtccatcc
     9061 ttcttctact gcaattgtca gttggtcaag aaggtggaac atctcttttt tgctttggag
     9121 ctgtgctcca agaatctttt tttcagaatc aaatagaaat ttgagtttaa ctttgctttg
     9181 atttattgct gtaagcttgt agtctacgta tcctgtttca attttttggg gataaaaaat
     9241 gctctcttct tctgtcaaac ctacgcttcc taagaaatag ccaaaccact gattgccgac
     9301 tggacgatag gttccctgat cctttgtctg tgagccacta attgtcttgc tgacatttcg
     9361 tgctgtacgg acagcgttat ttactagaga aacatacatt ttttcatttg ttggacgaaa
     9421 agttacttgg atcgcatcgc caatcgcata aacgtctttt tgagacgttt gcaagtagtc
     9481 atctacccag atcgttccat catcattcaa tgttaattgt tcttcccaca tctgattgtc
     9541 gggacgagaa tgattagcca gcaaaacaag atcatagttc tcctttcttt tttccgtctc
     9601 tacaatagcg gtttcatttt cggtcaaaga cacaacttgt tcttctaaaa aaagtgaaag
     9661 cccttgattt ttcatttcct ttactaacgg ttctgtcatt tcaggatcta gataacgaaa
     9721 aagaattgac tttcggcttt cgtaaagatg gatttgtttt tttcttgatg ccaacccttc
     9781 agctatttcg attcctgctt gtcctgcacc aataattgca attttctttg cattcatgat
     9841 cttttgctga agagccgtta cgttttgtat ctttttaact gggtgaataa gcgttgattc
     9901 tgcagaaata tttcgaaact tttgatttga accagtcgct agaatcaaac gatcaaaatc
     9961 caattttttt cctcctgcta aaacgacttt tttttctttc aatcctatta ctgtcttttc
    10021 tgtgtaaaca tggattcgat agcggtcagt cagttcttct ttcgtgatcc aagaaagttg
    10081 tttttcactg gtaacctttc cttttaatat caaattgaac ccgctgggaa tccatcctaa
    10141 cttcgattgc ttttcaatca aatatatttc acttgttggc atcaattggc gaagataaat
    10201 agccgcatga atccctgcaa acgacccgcc gacaattaca atgttcatct gcattcctct
    10261 ttcttataag gtgttctact gctaacttct caatcagaaa acacagtatt cataactcgc
    10321 tttcaacctt atgatacata gcgatgcagc agaatcaacc ctctccatca taccgtgttc
    10381 taggtctgct tctcaataat gtactagtgg acctaattga agagtatcat ttctgaagag
    10441 atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg ccttttacgt acctctgtcc
    10501 aaataggaaa cagaaaagaa cgaaaagacg agttttagcg tgtgtaatgt aatagttaga
    10561 atagacacat catttatttt taattcttta atgcttcaac aactgcttcg cctaattgat
    10621 gagcagattg aggattttgc cctgtaagaa gccgcccatc aatttgaacc acttctgaaa
    10681 aagctgctcc agactgatag tttgctcctt tttctttcaa agcatcttct gctaagaatg
    10741 gtacttcatt cgttgtacca tttgcttcct cttcttcatt actgaaccca gtcactgtac
    10801 gtccagctaa aatagatttt ccagactcat ctgtaactgg caacaatcct acaacaccgt
    10861 gacaaacagc tgagaccagc ccttcatttc gatagatttg tgaagcgact ttcccaagat
    10921 ctattgcttc tggaaaatcc cacattgttc cgtggccacc agcataataa accgctgcat
    10981 aatcatttgc atccacatca tcaggacgta aggtatttcc caaggcccga tttctgaaat
    11041 cttcatcttc ataataggcc caatcgattt catccatatt caaaacactt tgcggttcta
    11101 aaggtacata tccgccttta ggactgacaa aatctgcatc gatcccattt tttactaata
    11161 catcataaaa atgagtcagt tcacttaacc acaaacctgt aggacgctcg atcttttcgt
    11221 acttttctac atttgttaat acgatcaata ctttcatttt cccatcctct ttctataaaa
    11281 attgcgttat tgataacgtg ctttcaagaa attatataat gggcttggca aataaagctc
    11341 tttatatgct tcttcaaaag gtttcgttac aaattcatcc tcacggtttt ccaataattt
    11401 ctctgcccag tcaggatcga cgatcatttg ctgtccaatt gctgcaagtt ctgcatattt
    11461 caatacctgc tctacatctt cgcgattgcg gatcccaccg acaccaacta aagggatctt
    11521 tccttgaatc gtgttatgga ccacttcaag aatcgattta tcgttgtatt tatcagaacg
    11581 agctattcga tcatacatat ttaatgaaac atgcagataa tctagttttg actcacgtaa
    11641 tcgttcaaga agccagatcg tgtcttcaaa acggatacct ggtgtttcat attcttctgg
    11701 tgagaaacga taccctacga tgaatggttt tttagcatat tgttcaaccg ctgagaaaac
    11761 cgttgttaat aattcttcaa taaaacgata acgattttcc aagctaccgc cccattgatc
    11821 ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc aaatacgtat ttgctccatg
    11881 caactcgatt ccatcaaatc ctgattcaat cgctctacgt gtggctttgc caaatgcttg
    11941 gattgtttca acgatttctt cttcactcat tgctcgtggt gtttccgcat caggacgttt
    12001 tgctgcaact gcacttgcag atacaggctg ttctccagat aaagttgctc gacttgtcat
    12061 tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct tctccttgga ttgctttcgc
    12121 tagctcactt aatcgaggaa tcatttcatc atgagcgatg ctgagttcac ctggccagcc
    12181 ttttccgata tcttggacat ttgcagcacc agtaataaca gcaccaagac ctttagaacg
    12241 ttgtttataa taagtgatct catcttcggt gatcactcca ttgaaaaagc tgagttgtgt
    12301 cgtcataggc gacagcatca atcggttctt caattgaact cctgaaggta aagtaattga
    12361 ttgatcaaat tgtgacattg tcattcctcc cataattcgc tctgattaac ctatcgagaa
    12421 tcatctatca ttaaaccact cgattagttg tacatgcaac taatttgctt aaaaaatttt
    12481 attttttttg taacttatct gttgcatcat tcattgtctg ggctaattct ttacttgctt
    12541 catttcccaa aactgctttg tatgactgat gaagttcttc ccagctacca tcgacttcct
    12601 tagcaaattg gcgtccttgt tctgtaagat aaactaatga caagcgacct tcgctaactg
    12661 ttttgacgta tcctttatat tctaattttt caacaaaacg agcaatcgtt gatggggcgg
    12721 aatagagcat ttgtgcaata tctttttgtg gtattccatc tttttctttt aatatcaata
    12781 aaataaaacc ataagttggt gccagacctg tccttctaaa cgcttcatca gctaatttat
    12841 tcaacatgcg atctagcttt ttcacgctaa agaaaagaca ttcatctaac gctttttcaa
    12901 ttggcttttc catttcatcc cctccattgg cgtacatatc atagtatgat ttttattttt
    12961 tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag atttgaaaaa aagaagtagc
    13021 catcttcaaa gaatttcggc ttgttttcga caaaactcct tctggaacac cgtgaatcag
    13081 aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa attttgtgaa aatggctttc
    13141 cacatttttg cataaatttg gcttaatacc gaaaaactgg cttttgaaca ccgtttatat
    13201 cagaggttgt gaaaaagccg tcctgcatca agtaataaga acagccaaac aaaaatagtt
    13261 cctcatattt tgtcgttttt tgagctaatg ccgatggtca gctgattgta caccgtttat
    13321 tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag gagcaatttc aaaaaacagc
    13381 ttctcatgtt ttatgactat ggtgacttgt gatcgaggag ttggcaggag aatactgttt
    13441 attcgtaaag aaggggctgt gacaagactt ttgtcacagt cccttatttc cttttattat
    13501 aggatttcgc catttgattg gatgacttgt ctgtaccaat caaatgattt tttctttaac
    13561 cgtttctttg tgccattccc ttcgtcatct tgatcaacat agatatatcc ataacgtttt
    13621 gacatttgtg aagtagaagc gctgacgata tcaatgcagc cccacgacgt ataaccaaat
    13681 aaatctactc catctttgat agcttccttc atctgctcaa tatgctgctt caaatagtca
    13741 atgcggtaat cgtcttgaac ggttccatct tcttctactt tgtctatagc tccgagtccg
    13801 ttttcagcta tgaacagtgg tttttgatac ctatcatata attgattcaa agcaattctc
    13861 aaacccttag gatcaatctg ccagccccat tcacttgatg gaagatacgg atttttaatt
    13921 cctccagtta ttaagtttcc ttggacccct tcccaatttt ctggatgagc actgactgca
    13981 ctagacatgt agtagctaaa agaaacaaaa tcaacagggt aggctgctaa gatttcttca
    14041 tcacctactt ctatttttaa gttgacacct ttttcttcaa acattcgttg cgtatacgct
    14101 ggataatatc ctcttgcttg aacatcacta aagaacaaag aagttctttg ctgctcaatt
    14161 gttttttgaa tatcatccgg atcacaagaa tacggaacaa gaagctgata gctgatcata
    14221 caaccgactt gtgaaccttt gataatttca tgacaagctt ttgtagctaa agcacttgct
    14281 ataaactgat ggtgtgcacc ttggtatttc gcttgttcca aatggggatg gttttcttca
    14341 attactccta cgctaacaaa agggtgatgt tttacacagt tgatctcatt gaaagtcatc
    14401 caatatttta ctttattttt atagcgtttg aatactgttt cagcgaaacg aacgtaaaac
    14461 tgaataagct ttcgattagg ccatccacca tattctgtaa ccaaatataa cggcatttca
    14521 taatgggaaa gagaaacgac tggctcaatc ccatatttta gacattcgtc aaacacttga
    14581 tcataaaaaa ataatcccgc ttcattgggt gtttcttcat caccatgagg aaaaattctt
    14641 gtccatgcga tagacatccg atagcattta aaacccattt cagcaaataa acggatgtct
    14701 tctttaaagt gatgatagaa atcatttccg tgtcttttag gataataata cttgctgttg
    14761 ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat gccattgatc tgtccatttt
    14821 tctttaggta aatcgctgtc gaatgtatac acatcagaca cagataatcc tttttcatct
    14881 acatcccatg caccttcaca ttgattggcc gctgtcgctc cgccccataa aaatccttct
    14941 ggaaattttt tcatatcgat ccccctaaac ttgctagttt tcttgtttga ttatttttct
    15001 tatgactagt aatgatattt ttgaatctac tactatttgt aacaataacc attgtggtcg
    15061 gatcatatcc ctctttgatt aattcttgca aatctacttc catcaataaa tcgcctttac
    15121 gtactttttt tccatcagat actgcacttt gaaagtattt gccatttaaa ttcactgtat
    15181 caataccgat atggatcagt atctctgctc cttcttcact gactacccca attgcatgtt
    15241 tcgttggaaa aactgttgaa acttccccgt ttacagaact gtagattttt ccgtccgttg
    15301 gaatgatcgc aaacccttct cctaaagatc cactagcaaa tgttggatcg tttacttcag
    15361 ataaatcaat tatttcacct gctgcaggag tacttaacac tatatccttt gaggattctt
    15421 cttcgatttc atccttgtag ccgaaaagaa aagttagcat acaggaactg atcatcgcta
    15481 agaaataact agcaactagc caaacaaatc cttcaccaat ataaatcggg aaagacaaaa
    15541 tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc accagctact ccaccaaata
    15601 tagctccaca tatacaagca atgtaaaatg gtcgtttata tttaagagtc acaccataaa
    15661 cagcaggttc agtgattcca aataatgctg tgataaatgc agataaagat aaacttttta
    15721 ctttcttatt tttcgttttc aaaaataccc caagtactgc tcctgcttgc gcagctacgg
    15781 cagtcataca tgctggacct agcgtatcac gaccgtagaa agaaagattg ttggtcatta
    15841 ctggtacaag tccccaatgt aacccaaaaa tcactaacac ttgccaaaat ccgccgataa
    15901 cagcaccaga taaaacaggg ctaaagttat aaacagctaa atatccagct gcaatcagct
    15961 gacttgcata atctgcaatt ggacctacaa ctaataaaat aattgggacc atcacgatga
    16021 tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg caagcttttg cgaatcttag
    16081 gctcaatata agaaagaacc catacagcta aaattgctgg aataaccgta cttgtatacc
    16141 ttgctagaat cacgggcatc cctagaaatc gcaaggttat gctatcacta tatgcactga
    16201 caatcgtagg ataaacgaga gctgctgcaa taaccatagc gatgaaacga tctgtattga
    16261 acttttttgc agcagtatat gccaaaaata taggcaagaa atagaaaaat gcatcagctg
    16321 ctgcattcaa aatgatataa gttcctgatt cagtagttaa taaattcaaa ctcgtacata
    16381 aggcgagtac tcctttgatc aaaccacttc ctgccaagac gcctaaagtc ggggcaaaaa
    16441 cagcggcgat catgtctata aaagagttta aaatgccttt tttctcagtt cctctctttc
    16501 cactagatgc atcatcttct agtttcattt gtttggataa tacgtcatac acttcattta
    16561 ctgcatttcc tactacgact tgaaacattc ctccagcttc gactacagtg atcacacctt
    16621 ctagttgctc taccttctcc ttatctgcct tcttattatc ttttagggta aagcgtaacc
    16681 ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc tccgacaaat cggacaatat
    16741 ctttcgctaa ttgtgaatag tccattttta cttccccctt gttaattaat gccgtagcaa
    16801 tccttcttac aagataagta tagttcttag aaaacggttt ctccattcaa ttagtacccc
    16861 ttctaatagc gaacgaaact gtacaatagt gtgtatggtt tacttagccg agcaaatcac
    16921 tcactgcttg aaagaaaaca agtatatcgt ttctcttata gtcaaaggag acaaaaaaaa
    16981 cacgcttgat cttcctttgc tgatgtataa gtggtatctc aagctgatac tctgtgattt
    17041 ttgtttctac ttggcttttg ataagacagc acaacaaagt ctcggcattt aacggaagca
    17101 taactaggtc agaactagga gagattagtt gctgaatgga atctatcttt ttatggattt
    17161 ttatttgatt ttcttctttg tagtaatgat caagcagttt tgttttcttt ttttcttcaa
    17221 ctatggtcag cttttcacgc aaaatctgat aaatctccca agattcaaac tcatctagaa
    17281 cactaggtag ataaatcaaa tcatcccact caaaaataag ctcttgctca gtagtcaaaa
    17341 ttgcatcata ttccgttatt ttgtttgatt gatttttgaa aagatacact ggttctatcc
    17401 ggcattcttt gatccacatg ttcatacttt gttgaatctg tttttttagt gtattcaaaa
    17461 tactaattgg ctggttacta acaatcagta tctccccttg ttttcgaaaa ggattcaacg
    17521 cttcggcaag atacaaaaca aaataagcgc tttcagctaa attgggacgt aattgaaaat
    17581 gtattgggaa gaaacgccga attaaataaa tttctaaagg ataacgggag atgatctttt
    17641 gtacaaaatg attcatggta ttatgtccgg catccatcct cgttttcatt tgtttgatat
    17701 gggttagcaa aaattctgtt tcatggaaca ataacgaaac aggaagatct aaaaaacgaa
    17761 taatcgtttg ctcaaatgct cttagattct tctctatttc tttgtttttt tcatcattaa
    17821 tcatcaaata atttaactct aatagtcgat tttcaattgc ttgtttttct tgattggaaa
    17881 aagaataggg tagttctgat gataactttt ttgtgagggt ctctaccatc ggaataaata
    17941 atgtatacct gtttgttttt tctaaaagtt cttctcttcg aagaatggtc aaaacgagat
    18001 accgagaaaa tcgtatgaag tcctctcctg aaacaatata attatatgat ataagaacct
    18061 cttttaatgt cttttggaca gtcggcatga tattggaaaa aattgtactg atttgttgag
    18121 gaaaccttgc gtgttggatc accgtttctg ttccaacaag agataagaaa attctttttg
    18181 aattttcttc agcaatgatt tttagtcctt ttgtcgacga aacttccagt tctaatttag
    18241 gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa tgatacggct gtttttgaaa
    18301 gaaagaaaat gtctgctatc ttttgcatac tgacataccc tttttgaaac aataagtacg
    18361 tcacaatagt tgaagctcgg aaatccatgc tggaatagat tttatgtttc gtcacctctt
    18421 gtatgatttg ctgtttagtt tcagattcaa ttgattctaa aaaataacct gaacgtttat
    18481 ttgactggat aatgattttc ttctcgtaga tagcaagcat atttttgatt tcagtttgga
    18541 tcgttttctt gttggtacct aaagcttcag ctaattcttc gcttgttatc caatgattct
    18601 tatttcttaa tactacaaga atttggagtt gtcgtttatt taagtccatt tgcttacacc
    18661 tcctctttgt tataaacata caggaaaaac ctctcaaatg acaatagtca cttgcagagg
    18721 tttaaaaatc atttgaagaa agatctatat tcaaggtttt taccattaga ctggataatt
    18781 tgtttataat attcaaaact atcttttttc aatctcctaa agcttccgct cccatcattt
    18841 tgtctatcca cataaataaa gccatatcgc ttcctcattt cccctgttcc tgccgatacg
    18901 atatcgattg gtccccacca taagtaacca atgatttggc aaccatcttt tattgcctca
    18961 gcaatttcct gaagatgtat ttttagatac tcacaacgtt ttggatcgtg gattcgattc
    19021 aattcatctg gtgcttcatc tagtccaatg ccattttcaa ctatgaataa tggtaattca
    19081 taacggtctg tcaattcatt catgacataa cgaaacccta gaggatcaat tggccaactc
    19141 caaggttcgg gagatttttc cttcaaaaag ggattatcga aaccatttgc tccgccagta
    19201 tctacgcgca tttctgcttc ttttttatag acagcgctac gataataact aaatgcaata
    19261 taatctgccg tgttagcttg caagcactgt tcgtctccat ctaacattac tggtgcacag
    19321 tcttcttcct tccatgtcct gtaaatataa gctggataat gccctcggca catgacatcg
    19381 gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa atacattctc tggatcacag
    19441 tcatatgcat aagtcgctaa tgcagaaaag ctacacataa tacccatctt agcatttgga
    19501 tcgattttct ttaataattg agtagcacgg gcgttcgcta gaaacatatg atgacaggct
    19561 tgataaatca ctttttttgt tagtgtagca atgggttcaa gtttatctct agcagggaat
    19621 gtaaccaatc cagcagctgc ataaggcata cgaaaagcat tattgatctc attaaaggtc
    19681 atccaatatt tcactttccc ttggtagcgt tcaaagacgg tctgcgcata aaattcaaaa
    19741 aagtcgatta attttcgatt ttgccacccc ccatactctc ctaccaatgc taagggggtt
    19801 tcataatgac taagagtaat tactggctcg atatcatatc gatttaatgt attgattagt
    19861 ctgtcataaa atacgagacc agcctcattt ggcttctgtt cgtctcctct tggaaatatt
    19921 cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta actccttcag taaacgaata
    19981 tcctcttcaa atcgatgata aaaatcaatc gcttcatgag aaagataatg gagtgattcg
    20041 tctaaagcaa tctcccaaat ctttttttct tcattccact gaatactagg atgctggttc
    20101 atgatcccga taagcgtatc tgtcacatta ggtaatttgc cgtcttccaa ccatgcacct
    20161 tcacattgat tagcagctac tgctccgccc cataaaaaat tttctggaaa tatttcctga
    20221 tccataacta tctctcctcg ctatcaacaa tttcttactt gaagtatagc tcgattcatc
    20281 aaaacaagat attctaacta tttcggcaga agagaagaaa aaaattgaat gctactcttc
    20341 atttttaata tttatatata acaaatacat aagacgatcc ccgaaagaaa acactcgtta
    20401 accatctaag caaacgattt attttacttc caacacaaat gcttcataag gtcgtaaatc
    20461 aactttgccg tctggtaact gtttcagagg ataatttgac acaataaccc ttaaagcttc
    20521 acgtggaata gtgaacgtct gcttttcact ggacatatta caaataacga gccattgctg
    20581 gtcttcatat atgcgagaat aggcaaagac atgctcaggc gtcttttcca ctaattcata
    20641 gtctccccag acgatgatgt catgttttct acgcaaatcg atcaatttct tgtacatata
    20701 aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg atttctttat aatttggatt
    20761 gactcgaagc catggaattc cagtagtaaa cccagcatgt tcacgggcgt tccattgcat
    20821 aggcgtccta gcattgtctc ttcctttcgc cttgattgat tccaagattt cctcttttgt
    20881 aaatccagaa gaaatccttt cgttatacat attgattgtc tcaatatctt ttgcttccgc
    20941 gatatcatcg attgggcaat tcgtcatgcc taactcttct ccttgataga tataaggggt
    21001 gcctttcatc atatggagca agatagcaaa catcttggca ctttcttttc gatacacatc
    21061 gtcatttccc caacgagaaa caattcgcgg aagatcgtga ttattccaaa ataaactatt
    21121 ccatccttca tcacctaaag atgtttgcca tttggataat acttttttca gttcagagat
    21181 actcaagggt ttcaaatccc atttgtcttt gccttcttgc tgatctaatc ctatgtgttc
    21241 gaattggaag accatggata attcatttct tttaggattg gaataaagtt tagcgatttc
    21301 ggtagttgct ccccatgttt cgccaacagt catcagattt ttgtcaccaa aagttgcttg
    21361 attcatttcc tgaagatact catgcagttt aggtccatta cttgtgattt ctttatccgg
    21421 ttctttgcca atcatatcga tcacgtccat acgaaagccc ccaatacctt tatcgatcca
    21481 gaaattcatc atttgataaa tctcttggcg aacctgttta tttttccaat tcaaatcagg
    21541 ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt ttttcatcca actgccaagc
    21601 agaaccactg aatattgagc gcaatctatt aggctctttt ccattgaccg gatcacgcca
    21661 aatataatag tcccgataag gattttcttt atttttacgg gcctcaatga accacggatg
    21721 ttcatcagaa gtatgattca ccaccagatc catgatgata cggatatttc ttttctttgc
    21781 ttcttcaatc agttcttcca tttcttccat cgtaccgaat tctgccatga ttgcttgata
    21841 attactgata tcatacccat tgtcatcatt cggagactcg tatactggag aaagccaaat
    21901 tgcatcaata cctaaatacg ccagataatc caacctggaa ataatccccc gcaaatctcc
    21961 aataccatct tgatttgaat cttgaaaact tcttggatag atttgataaa caactgaatg
    22021 gtgccaccat ttttgttcca tgattttcct cctataaatg gatgtttcta ttttttgctt
    22081 aatcatttat tttagtctct ctcactaaaa attgtaacgc tttcaaatag ctattgcacc
    22141 ttgttatcgg taacagaata aaacaaaaaa acagctgagg aaattccctc aactgttttt
    22201 tgaaaggatt gatttatgca gggaagaatg ctgatttcac aacttcttta taaacggtgt
    22261 tcactcagct gtcccttggt attagctcaa aaaacggcaa aatataagaa actatttttt
    22321 cgtttttttc tctaatactc aggccagaac gctgatttca caacctcttt atttcacatt
    22381 caatatccac atgaatccca caaatacaaa gaacagacca tacatgattg gagatacttc
    22441 ttttcctctt ttagctgcta acatggtaat tggatagaag ataaaaccta atgcaatccc
    22501 atctgaaata ctataagtca aaggcatgcc gatcaagatc aagaatgaag ggatcgcaat
    22561 ctctaagttt ccccattcga tttgtcgcaa agattgtgcc atcaatactc ctaccataat
    22621 caatgctggt gcagttactt gtgaagtaac aactgatagt aatggagaaa agaacaagcc
    22681 taacaagaag aaaatccctg tagtaatagc tgtgatacct gaacgtcctc caacggcgat
    22741 accagctgat gattctacaa atgcaccaac aggagaagta ccaaataagg aacccgcaag
    22801 catagctgtt gaatcagaag ctaaagcttt ccctacacga ggcatcttgt tgtctttcat
    22861 gaaacctgct tggtttgcta atccaactaa agttcctgct gtatcaaaga aagtcacaag
    22921 caagaatgtc agcactacga cccacatttg aagagaattg atgtccccta catgttttaa
    22981 tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca acaatttcag aaggcattgg
    23041 aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc atcccgatga agatgccacc
    23101 tggtacacgc cgaacaagca agatagcagt tacgactaat ccaaaaatcg tcaaccaagt
    23161 agaaccaacg ttgagtgatc ctaatcctac taatgtagat tcattcgaaa cgatgatccc
    23221 accttcgctc agaccaagga aagcgataaa tagaccgata ccgccagaaa tcgcatattt
    23281 caaatctgct gggatcgcat caatgatcat ttcccgcagc ttgaagattg tgatcaaaat
    23341 aaaaatcaaa gaagcaacga atacgccggc taatgctgtt tcccatggaa tccccattcc
    23401 taaacaaaca gaataagcaa aaaaagcatt gatcccaaga gcaggtgccg ttccaattgg
    23461 ataacgggct aataccccca ttaaaacaca gccgatcgca ctggctaatg cagtcgcagt
    23521 aaacactgct ccttcatcca ttccagaagc acctaataca gttggattta caaataagat
    23581 ataagccatt gagataaatg ttgtaaatcc tgccagtacc tcacgtttta ccgtggtgtt
    23641 caatttttct atttcgaagt aggaaagtat tttttccttc atcttccatc cccctaaatc
    23701 cgaacattat aaataaagtc cgttaaaatt tcgatttatt aataaaacaa cgtgttttat
    23761 catatactaa aacaaaacat aagtaaacaa aaaattcgga ttttaagcaa aatccgaacg
    23821 ttccatctga ttaaatccct ttatctcaaa gttcatgaac attcactttg gattacaaga
    23881 atttctttat tgtgttaata aagtttcttc attctagtct caaaaatcaa tagtgtgatt
    23941 tgattttacc atttcgtttc tgttcttctt tgatgaattc ttttgtatcg acaggattca
    24001 gattatttag tccgccaaaa cctgtatcga ccatttttcc ccagcctttt gtcttctttt
    24061 ttcctgattt gagattcttt gctcgaagct tctcactatg ttttgctcgc cgatacttca
    24121 aataaccttc taatcttccc attagatcca ctgtttttcc tcctttttat tcaatcagac
    24181 gactcagctt ttcctataac aaaataattg ccttcagggt cagaaaaatt gaaagtcaag
    24241 atgccattgt tatctgtgat ttctcctggt tcttccatgt tctcgtagat agtttcaaat
    24301 tcatccgtaa aaaatactaa agaaggcggt ggtcctaata cctctggtga aaacttctgc
    24361 acaaatactt ttgggaatac gcctaattct aattcttttg agatgctgag tacaatactc
    24421 ttaaattctt ctggcaattc gattgtttca gaaatttctg caccaatttt ttctacaaag
    24481 aatcggcaaa tcatctctac gtcattgaca taaagcatca cacgtgtttt ttgtatcatc
    24541 atttgaccct tctttctatt tgattttctt tcctgtctat aaactgattc cattatatca
    24601 cgaaagcgca aacaccttgt gtttgatact attcaagaaa aataaagcaa tagtttctag
    24661 gattgctttc ctccacaaac tttttttaca aaaaaaaaac ggcaatgaat gccgctcttc
    24721 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
    24781 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
    24841 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
    24901 gtcagcatcg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
    24961 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
    25021 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
    25081 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
    25141 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
    25201 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
    25261 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
    25321 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
    25381 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
    25441 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
    25501 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
    25561 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
    25621 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
    25681 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
    25741 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
    25801 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
    25861 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
    25921 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
    25981 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
    26041 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
    26101 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
    26161 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
    26221 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
    26281 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
    26341 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
    26401 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
    26461 tttattttgt ttgtaagact agttcacctc tttttgttgc aaatgcaact attttcaata
    26521 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
    26581 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
    26641 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
    26701 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
    26761 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
    26821 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
    26881 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
    26941 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
    27001 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
    27061 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
    27121 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
    27181 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
    27241 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
    27301 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
    27361 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
    27421 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
    27481 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
    27541 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
    27601 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
    27661 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
    27721 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
    27781 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
    27841 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
    27901 tgccataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
    27961 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
    28021 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
    28081 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
    28141 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
    28201 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
    28261 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
    28321 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
    28381 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
    28441 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
    28501 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
    28561 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
    28621 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
    28681 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
    28741 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
    28801 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
    28861 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
    28921 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
    28981 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
    29041 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
    29101 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
    29161 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
    29221 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
    29281 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
    29341 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
    29401 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
    29461 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
    29521 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
    29581 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
    29641 ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt aaagatcgtt actagcaaag
    29701 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
    29761 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
    29821 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
    29881 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
    29941 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
    30001 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
    30061 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
    30121 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
    30181 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
    30241 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
    30301 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
    30361 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
    30421 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
    30481 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
    30541 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
    30601 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
    30661 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
    30721 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
    30781 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
    30841 tttcgatgat aaaattgata acgcccatgt t
//
LOCUS       DACEDO010000030        30338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEDO010000030 DACEDO010000000
VERSION     DACEDO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.030"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(235..1545)
                     /locus_tag="IWO52_001403"
     CDS             complement(235..1545)
                     /locus_tag="IWO52_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0355679.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1557..2975)
                     /locus_tag="IWO52_001404"
     CDS             complement(1557..2975)
                     /locus_tag="IWO52_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0355680.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3215..4783)
                     /locus_tag="IWO52_001405"
     CDS             complement(3215..4783)
                     /locus_tag="IWO52_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0355681.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4787..5197)
                     /locus_tag="IWO52_001406"
     CDS             complement(4787..5197)
                     /locus_tag="IWO52_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0355682.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5194..6117)
                     /locus_tag="IWO52_001407"
     CDS             complement(5194..6117)
                     /locus_tag="IWO52_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0355683.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6114..6992)
                     /locus_tag="IWO52_001408"
     CDS             complement(6114..6992)
                     /locus_tag="IWO52_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0355684.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6979..8316)
                     /locus_tag="IWO52_001409"
     CDS             complement(6979..8316)
                     /locus_tag="IWO52_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0355685.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8319..9422)
                     /locus_tag="IWO52_001410"
     CDS             complement(8319..9422)
                     /locus_tag="IWO52_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0355686.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9496..11004)
                     /locus_tag="IWO52_001411"
     CDS             complement(9496..11004)
                     /locus_tag="IWO52_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0355687.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10970..11662)
                     /locus_tag="IWO52_001412"
     CDS             complement(10970..11662)
                     /locus_tag="IWO52_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0355688.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11684..12658)
                     /locus_tag="IWO52_001413"
     CDS             complement(11684..12658)
                     /locus_tag="IWO52_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0355689.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12958..13830
                     /locus_tag="IWO52_001414"
     CDS             12958..13830
                     /locus_tag="IWO52_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0355690.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14288..16486)
                     /locus_tag="IWO52_001415"
     CDS             complement(14288..16486)
                     /locus_tag="IWO52_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0355691.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16718..17710
                     /locus_tag="IWO52_001416"
     CDS             16718..17710
                     /locus_tag="IWO52_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0355692.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17770..19191
                     /locus_tag="IWO52_001417"
     CDS             17770..19191
                     /locus_tag="IWO52_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0355693.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19184..20683
                     /locus_tag="IWO52_001418"
     CDS             19184..20683
                     /locus_tag="IWO52_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0355694.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20722..22116
                     /gene="uxaC"
                     /locus_tag="IWO52_001419"
     CDS             20722..22116
                     /gene="uxaC"
                     /locus_tag="IWO52_001419"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0355695.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22275..22958
                     /locus_tag="IWO52_001420"
     CDS             22275..22958
                     /locus_tag="IWO52_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0355696.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22960..23796
                     /locus_tag="IWO52_001421"
     CDS             22960..23796
                     /locus_tag="IWO52_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0355697.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23807..25561
                     /locus_tag="IWO52_001422"
     CDS             23807..25561
                     /locus_tag="IWO52_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355698.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25837..26178)
                     /locus_tag="IWO52_001423"
     CDS             complement(25837..26178)
                     /locus_tag="IWO52_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0355699.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26246..28417)
                     /locus_tag="IWO52_001424"
     CDS             complement(26246..28417)
                     /locus_tag="IWO52_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0355700.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28628..29485
                     /locus_tag="IWO52_001425"
     CDS             28628..29485
                     /locus_tag="IWO52_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0355701.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29556..>30338)
                     /locus_tag="IWO52_001426"
     CDS             complement(29556..>30338)
                     /locus_tag="IWO52_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0355702.1"
                     /translation="LSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDNY
                     GYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLTV
                     GTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIEA
                     RTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQQ
                     FVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9069 a         6249 c         5369 g         9651 t
ORIGIN      
        1 ttatcgtttg ttatttgtca agaacttttt tgaagttttt ttcgatagaa tcaagcttcg
       61 caagtggttc tttttaacga cttagttatc ttatcacgtc taaaaacatg tgtcaactct
      121 tttttggaat tttcataaaa tgtttcaatg aagcgcgaca acgttgttta atatatcatt
      181 aaaaaaaata aaggtcaagc taaaatatta tttaagcttg accttattat aatttcaacg
      241 attgtcaaaa gtattgtttg cagctgtgtt gccagtttct atagataaat cacagctttt
      301 gaaacataag ttttgtactg cttcgattcg cccgaattct tttgcagtta tttggcaatg
      361 agaaaagcag acatcttcta taggcttttc aggaaatgct actaaatcaa aagctcggga
      421 aggaagtgag taacctggag aaaggcatgc ctgtacgttt ttgatgtgta tgtctttgac
      481 tttcgtcatt tgctttgatt caggtatttc ttcagccact gcttgccaac tttctggcat
      541 ttgagcaagg tcattgctat tttttctatt gtattcatta tgccagtcca ttatccaaga
      601 aaaaggaaat tgtacatcaa tcatattcag attttctacc ctgatatttt cgatcactcc
      661 ccctctttct tttgaagatt tcattcgaat accacagtct gactgaaaaa aatcgatatc
      721 gtgaatatat acgtcactaa ctccagcact aacttcgcta ccaatagtta caccgtatcc
      781 tgaacggatt ttacatctag caacctcgat tcttgatgat tttcgattga ctcttgcacc
      841 attgccatct ctgccagact taatagcaat acaatcatca ccacaggcca aatcacattc
      901 atagacacgc acatttgttg aagaatcaat atcaatccca tctgtacttg gtccatcatt
      961 gttgcggatg attacttttt ctactaatac atcgttagaa taagtgattt gcaagttcca
     1021 aaaacctgat ttctgaaaaa tcaaatcacg aacaacaaca tgttctgcat gatacagtaa
     1081 acaagcgcgt ggacgtttaa tagcataatc cgcaatccaa cgaagtccct tttgatcgta
     1141 ttcagctctt gtcccgcttt tttgatcttt tccccaatac agctcccacc aatggtctcc
     1201 ttgaccgtcg attattcctt ttccttctat aagaatatct ttcgccgaga gaacattcaa
     1261 aatcgctgct ggccatttca tttctacgcc ggctactcgt gtatcgattt ctggaaatgc
     1321 ttcgatatct gttctcccct tgaggactgc accttcttca aagataagat gactcttatc
     1381 ttttaaaaaa agggctccga ctaaataaat gccttttgga ataaatacag gtaagtgttc
     1441 tttcgcacct tgatcgattg ctttttgcag tattattgta tcatcttgtt gagtgtcgtt
     1501 tgttttaaag ttcaaagcat tgataatccc tttttttgtc cgcaatacgt ttctccctat
     1561 ctttcattga tttttttcaa ttcttcacca ataaatacag tttttgaacg attcagtggg
     1621 taacctacta gcaaaatgat tgcggataca aataagcaga atgctggtaa caagtttgcc
     1681 atcgtataaa tcctttcttg tactaaaact gattggatgg ctccacctgt tgtagacgat
     1741 tggtaaccaa tgagtgccag cataaatccg cctaccgctc ctgcacatgc ttgtcctact
     1801 tttctcgcaa aagaatttac tccatatact gttccatctt ctcttaagcc tgtcttatat
     1861 tgatgataat caatcacgtc agtaatgaat gcccagacca tcaaattgaa taaaccagat
     1921 cctaaagtag cgataaataa aagaatcaga tatacccatg catttgttat atgaaggaaa
     1981 tacatcagca agtacataca tgaagaagca agtaacgcaa atacgcttgc ttccttttta
     2041 ccaaataatt ttgtcagctt tgtagcaaat ggtgctaaaa ctactactgt tccgtaagta
     2101 aataaaagag caacagacat tgcttgttta ttatggaagt aatcattgaa taaataagtc
     2161 atattcgtcc cagctaaaat ctgattgatc acaatgaaaa tgtcaacaat gactaaaata
     2221 atcagtgctc gattagctaa caaacctttt accaactgag aagcagatac tttttcgttt
     2281 ttgacaactc ttactctttc agtagtcaat ttacaagtta acatatatgc gacaaatgct
     2341 attgcagcac atccgattga aatccaaaag aaatgattcc ctgaaacgac ttgttgtcca
     2401 ctcttgttat gggtatacat taaaatcgga atgaagaatc ctgtagttga tccgccaatc
     2461 gcagaaccaa tgcttcggaa cgtggacaac gaggtgcggt cattaggatt tgagctaatt
     2521 gctgctgcca ttgctccata cgggatattg attgttgaga gaaaaatccc ataaacaatg
     2581 tatgaaacaa aaatataagc aatcttcacc gtcattggaa gatgttggac gaatggcaaa
     2641 aacaaaatga cagtcactaa gcaaaatgga tacttcattt ttctgatcca aggaataaat
     2701 cggccagttt ctgttaactc tgagacatca cataatcttc caaccatcac atcagcaaac
     2761 gcatccaaaa ttctcgaaat caagaataac gtacctacga ttgcccctga aacgccaaga
     2821 acattcgtat aataaatcat caagaaacta ttgactaaac ctaaaataaa acagttacct
     2881 agatcaccga acatgtagcc tatcttatcg gaaatcccaa attccttttt ttcttttttt
     2941 attgtatttg ctgtaacagc attttgtgaa agcatttgaa ttcctcctac ctcttctagt
     3001 aaacgttttc aaaattataa ctcttttttt cttgttttta tattcacaat gttggctttt
     3061 tattgcatta tcttgacaga taaaaaacaa aaaataatct aaaaacattg atgcataaga
     3121 ttgataactt tttttacata acaatatata caaacaaaaa aattcactaa ctagtgaaaa
     3181 taagttagtg aacctcattt attaaagaat taaattacag atgatctttt ccatcatact
     3241 tgaaaaactc aaaatcagca taacttcctc tttttttatt tagatcatgc actgctatcc
     3301 cgataaaatt tccagtaaac cctcctgaga gtatacacat gtttttttgc ggacctactt
     3361 tcttccattc ttcagttgat acgtctttca aatgaaactg tgctgtctct ttctttacag
     3421 tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct atttgtgcta
     3481 ctccgttttc tgattctatc atacgtaggc attttccctt gtcctcattc caagtaacat
     3541 aacaataaat ataattggat tcatttaagt acagtaccag tccagccatc tgattaaaat
     3601 tattaggcat gtattcaaat gctgtctccg catcaaaagt aaaatccgtt tgccgaatag
     3661 ctaacaaatg atgttcgaag gtagattgaa gagattcacc ggaaatcaga cgtaaaaaac
     3721 ctggtctaca ttttgtatca caccaatcag catttggcaa aatgcgtctg gcattccagt
     3781 tcttctttag ctctccttca aatgtatcca taaactgatg atctttttct tgttgtacct
     3841 cttcatttgt ctcaatcatt atttcttcca tcggagttgt ctggctatct ttaagtctta
     3901 accaatcgtc ggacgtccaa tacacttctt gaatagcggt ttctcttccc aaaatagccg
     3961 caccgtttaa tggtctggta caaagatatg tcatgtacca tcttcctgac ggggtttgta
     4021 ctaagctccc atgtcctgta cattgcaatg tagaagcagg tttgtcacta gctgtcagca
     4081 taggaaaacc aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg caaactgtaa
     4141 ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa ataataatac ccgttacgaa
     4201 aatacaaatg tggtgcctcc gttttcgcta attctgtacc tgcaaatatt ttatagatgg
     4261 gaccatcaag cttcttcttc tctgcatcat attcttgtaa tacgataccg ctggatttat
     4321 tcggtgtgtc cagacgataa tcccatagtt cattgacaag ccattttttt cccgtttcaa
     4381 gatcatggaa caaagaagga tcaaagccgt tactatttaa ataaattggt tcggaccaag
     4441 gacctgtgat ctcagatgca gtgatcaaat aattgtggac atctttaaac ggacggcctg
     4501 cacttttgac atctgtgtaa acaagataaa acaatccatc agcataactg atctgtggcg
     4561 cccatatact tccatttgta ggatttcctt gcaaagatac ttgagtggtc aaaatatccg
     4621 ttgtatgtga ccagttgact aaatttttcg actcatatac tcggattcct ggaagccatt
     4681 caaaagagga gacaataatg taataccgct ctttcactct taatatatta ggatctggat
     4741 tgaatcctct taaaatagga tttttcactt tgatagtctg catgattcag gctccttttc
     4801 tcattcttac tcgatataaa ctgaatgccc cagctgcgaa ataaaaaaat gcaaaaggaa
     4861 acgcaataaa ctgagtgcca atcaacaata tgaatcctgt tataaaatac aaaccagtgg
     4921 cagttgttaa gtattttcct ttagcagtca aaaacaatgc tactgctaaa aaagcaagtg
     4981 aaataaacaa cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt
     5041 ctaaagacaa agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg
     5101 cagacatatt acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac
     5161 cgaccattcc tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa
     5221 atgtatccat tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt
     5281 gacagtctgt tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc
     5341 acctatctta cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata
     5401 gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt
     5461 ttgatttttc ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg
     5521 gatatgacac tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc
     5581 aatccagcaa tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca
     5641 aaaaggagta ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata
     5701 accagatagt cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc
     5761 ctgtcctacc ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac
     5821 attcgatcct tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag
     5881 atactcctgt tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt
     5941 aaaattggat aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac
     6001 ctttggatgg ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa
     6061 atccgtcggg gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa
     6121 tgctccctta tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct
     6181 gttaaagaaa aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc
     6241 ccttttctca gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag
     6301 ctaagacgag ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat
     6361 agatttggca catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtatt
     6421 tcgatgtcca cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc
     6481 acccaccagc ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct
     6541 gtgtcttccc ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc
     6601 cataacgttt ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg
     6661 cgttcttcaa atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact
     6721 tctgcaaaag atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact
     6781 gaaaaatctc cgccgtgaga atgcaggtaa aggaccggcg gaaacggtcc ttctttttct
     6841 ggataagcat agagggctgg tactgtttcc aattcattta agtacaattg atagtattca
     6901 agcataaaac cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta
     6961 gatggagcac ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc
     7021 atccatattt tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc
     7081 taatagaaat gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc
     7141 cgcaaagcca atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg
     7201 aacaccggga actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg
     7261 tatgaattgt tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt
     7321 gatattcgaa aaatggatcc ttctgaatgc tggtgtccgt tcatctatcg gataagcctt
     7381 tttttcccat acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat
     7441 aaatggacac atcacattat ccatgactat attggatact cgaatatctt caacgatccc
     7501 gcctcgacct cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt
     7561 tgaaatggta atattacgaa tactaccact catttcactt cctaatacga caccgccatg
     7621 tccatggacc atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt
     7681 atcttcagtc cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact
     7741 aatacgcaca tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg
     7801 gttcaagatc gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca
     7861 acttggtgac tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact
     7921 catcaattta ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca
     7981 tttttttcca tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg
     8041 agcatagata caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac
     8101 aggataatct tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc
     8161 gacattgctt tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt
     8221 cccgcctccg tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc
     8281 atcaattgat gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc
     8341 aatgcttgca agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg
     8401 tagtaagcat atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt
     8461 ttattcaagt tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga
     8521 tgtgatttct tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct
     8581 ttagccattg cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt
     8641 tttgtttctg ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat
     8701 gcagccagtg tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt
     8761 aattcatagg tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga
     8821 tgggatagac ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga
     8881 atcagcaaat ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa
     8941 acatcttggt aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa
     9001 cccatatata agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct
     9061 ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta
     9121 tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg
     9181 tcgatattgt attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag
     9241 tcgatattgt cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat
     9301 tggaaccctt taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct
     9361 tcatataagc gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc
     9421 attatttgct ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg
     9481 tcccaatcgt ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat
     9541 tcgatcgcct ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag
     9601 ctgtcccatg atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac
     9661 ttcattgttc gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt
     9721 ttctttgatc aattgattag agaattcttt ttcagggtct gtatctttga aagagaacgt
     9781 cacttcacta ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc
     9841 gataaattcg atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt
     9901 catcaattta tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa
     9961 ttgtgcttcg tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc
    10021 ttctgaccaa acatgataac cgccgccttc accatctgag atcttgttta caggcactaa
    10081 ttccatatca tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc
    10141 agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac
    10201 tgcaaagtct tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt
    10261 catatactct tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc
    10321 atttggtgct ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg
    10381 cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt
    10441 aaattgttta gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt
    10501 atccaaccag tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac
    10561 gccgtacaag gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa
    10621 atttttatag tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt
    10681 gcggacagaa gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc
    10741 agaagccaat gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt
    10801 cagttccgta tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc
    10861 atgatgcaaa atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact
    10921 gttacttgaa ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa
    10981 ttttacgagc gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt
    11041 tttcttctgt tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg
    11101 ctgtccacgt gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca
    11161 ttgtttggat gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat
    11221 gaagatgtga gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat
    11281 cataatgagc catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa
    11341 tatacaaagc gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa
    11401 tcccgatggc gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat
    11461 atgtgaagta cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca
    11521 ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta
    11581 aaccgcccca cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta
    11641 ttttatctaa tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt
    11701 ctcctagctt tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt
    11761 taaacaatcc cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa
    11821 tataggtgtc gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat
    11881 gctcgaaccc taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg
    11941 gacgaattgc cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt
    12001 ctgcagcttc atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag
    12061 accagcccaa ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg
    12121 atgttagaaa actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc
    12181 cgtcaacatt caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag
    12241 ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata
    12301 gtaaagacaa aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca
    12361 acgtatttct taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga
    12421 aaccgaccca aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa
    12481 cgagtccccc cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca
    12541 tcacatataa aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct
    12601 tttcttcttt tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa
    12661 gcccctcctt agttttgtga atgtttgctg cttatgactt catgatagat aactatctga
    12721 attacgtata taatcatttt taccgtaagt aaaaacgctt acatagcaat gatttttgtg
    12781 ctatacaaaa cgataaagtg tattttttca caaataaact acgcatagca aaccttctat
    12841 ttttttctat ctgacactta gcataggaaa tgattatata cgtaatcttt caccgatgat
    12901 aaattgaaca tatagaaaca gaaacattta atttattttg aggaggagcc tatctatatg
    12961 aaaaagaaag tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt
    13021 ttgctagctt tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct
    13081 acacaagaag tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg
    13141 tatcgctaca ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt
    13201 acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc
    13261 agaaagtatt tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc
    13321 agcgggggga tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac
    13381 tgggccatga tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt
    13441 ttccaagcct tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt
    13501 ggtgtatttt tcaaaattat gctgccatta gcgttaccgt ctattgctac tatctcgctt
    13561 ttttatgctg ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca
    13621 tctaaatggc cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa
    13681 gcagaaagta gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc
    13741 gttattgcaa cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa
    13801 ggagcactcg taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg
    13861 tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt
    13921 tgtgaaattt cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt
    13981 gtgaaagaga cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta
    14041 acccacagga gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat
    14101 gccttattgt cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca
    14161 agacttttgt cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac
    14221 aaatactagg gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc
    14281 tcttgattca caaatatttt tttcgatatt ctcccggcgt aatcgattcc ttctttttaa
    14341 agaaacgaat aaaattttgc ggatttcgat attgcaatct ttctgcaatc tcttttacgg
    14401 agaggttagt ctctttcaac catttttttg ctacctctaa acgatgattt tgaacaaaat
    14461 caccaaattt ttctcctgtt tcctttttaa atacattact taaatagtta gggttataat
    14521 gcaacctatc agcgatcaca tctaaagaca gatcacgatc atattctgtg tagacgatat
    14581 gcataatatt ctccgacaaa tttttaaatt catgatcagt gattgcttga gagcattctg
    14641 tgattggcag aactaattgt tctaaaacaa gtttttctag ttctttggga tgataattgt
    14701 tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt ttttgaatct actcccaata
    14761 actgtcctaa ttgaacgagt tcattgatca agcggaccga aactacttcc cgactcaaag
    14821 gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc ttcacttcct
    14881 gatttccaga acgaatggct tcaaacaatc gattttgttc ttctatcgga tatctaataa
    14941 gtgttttttc atgagcagca ggaacgattt cctgataaaa aataattgaa cttggaccag
    15001 tatttacacg ataatacagg gcttcttttg cccgatcaac cgattgcttg ctttcattca
    15061 gcgtttcgaa ccggttactg atgcctatac tgatagttaa attcaagtat tcttttacac
    15121 gtttttgaat gagtcggcaa tactccatga cttttttgct tgcttctgca tcatctttat
    15181 ccattcgata aatagtggct tgcatttctt cattcaaaac gatcggcagc attcgatttt
    15241 cctttggcac aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc
    15301 caccgtgact atcttctaac agatcgattt gaaccaaagc tgtataatag cagtcatttt
    15361 gagaactata accaaattgc tgcaaccgct gatctagttc tcgcctttct actcgatttc
    15421 taaataaact aagtacaaat agcgtttcca gctgtggctt ttgggtaact aagtgtgcac
    15481 ttaacgcttc tttttctcct atgatccgac tgactgattg agcgaccaaa tctagttctt
    15541 tcccactgaa tccttttgat ttaagattca gacgttcctg tatttgagaa atcggacggg
    15601 caaatctgtc cgaaaataag taggaaagaa gaccaatgag tcctaccagt gttaaagaca
    15661 ctgcaactag cccgattctc agattgcgga tcattgcgcc aattgcacta gagtcaactt
    15721 ctaatctgta aatccaatta ttataatctg atttcagtac tatatacgtt ttatcttttt
    15781 ctcttatcac tttcagctga ttgtaatcta gatccacgtc cgttaataca gttggttgct
    15841 ctttattttt tactgtctga gtagtaaaaa ggttgccaac ttcgttttca atagaaagta
    15901 tgtcttctcc actttcacca acaacttgtt ctattgtatg tttctctatc gtagcaattc
    15961 ccaatgcata tttttctttt tcaaacattg gcaaagaaat caccatttcg attcctttgt
    16021 ttttcttttg ccaaaataaa ttatttttgt tatttatgta cttctgttga tacttttcaa
    16081 tttcctcttc ggtcagttga ctcaacgatc cattgattac tccccattta ccagccaaac
    16141 tgactaaatc ataattatta tcatccataa caatcgtttc tataaaattt agttctttcc
    16201 tgatttcaga ataagtggag taatctgtat atgtcagcgg atgcttgaat atgtccgaga
    16261 aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt ttccattcaa
    16321 cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc gattcgatcg
    16381 attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg gctcctaata
    16441 aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc ttcctctcct
    16501 ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta attaaatgag
    16561 cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat ttttaacgtg
    16621 tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat atgatgaatg
    16681 cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca aagatgtcgc
    16741 taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca gcagtttaat
    16801 aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt attcgccaaa
    16861 ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat ttttctcaag
    16921 tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca tccattcagt
    16981 attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt taacagaaat
    17041 caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg atcagccttt
    17101 catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga
    17161 tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg cagtagatta
    17221 cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa attttcgttc
    17281 gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa
    17341 tccagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata
    17401 cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat
    17461 tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat
    17521 cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa
    17581 gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag
    17641 gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa
    17701 ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag
    17761 gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa
    17821 gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag
    17881 ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt
    17941 cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc
    18001 aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct
    18061 tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc
    18121 tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc
    18181 ccacagagcc tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa
    18241 gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa
    18301 atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa
    18361 aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa
    18421 gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa
    18481 ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa
    18541 caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg
    18601 catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa
    18661 actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat
    18721 ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa
    18781 atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat
    18841 agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa
    18901 aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc
    18961 gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct
    19021 gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg
    19081 tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa
    19141 ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca
    19201 accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa
    19261 acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg
    19321 tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc
    19381 aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa
    19441 aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta
    19501 cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg
    19561 taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt
    19621 caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa
    19681 gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc
    19741 ggacgcagta aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa
    19801 caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt
    19861 tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct
    19921 tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg
    19981 tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc
    20041 attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat
    20101 gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat
    20161 cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga
    20221 aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg
    20281 ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat
    20341 cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc
    20401 tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg
    20461 ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca
    20521 ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc
    20581 gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga
    20641 cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat
    20701 aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg
    20761 gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac
    20821 ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta
    20881 ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc
    20941 acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt
    21001 atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac
    21061 ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca
    21121 gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca
    21181 acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat
    21241 ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt
    21301 aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa
    21361 ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc
    21421 gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt
    21481 tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcgtaca
    21541 gaaacactga tacatttgac ccacttctat cattctcata attggacgat gcagctgcac
    21601 atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc
    21661 tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca
    21721 gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct
    21781 gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt
    21841 tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct
    21901 gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc
    21961 tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg
    22021 cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat
    22081 acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa
    22141 tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg
    22201 accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata aacctttatg
    22261 aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg gcagaaaaag
    22321 atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat ttttcacccg
    22381 agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt atccaggaag
    22441 gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt gtacaatttg
    22501 gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt agctatttaa
    22561 aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga
    22621 catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat atacttggag
    22681 actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt ttagatgtgt
    22741 tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa aaattctacc
    22801 tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac aatacccact
    22861 ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata aaagaatccc
    22921 gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt aaatttaggc
    22981 atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag
    23041 cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca acttaaatta
    23101 actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa aaacaatatc
    23161 gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga agttcgtgca
    23221 caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca agccaagatg
    23281 attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt tactgaagaa
    23341 gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga aaattttggc
    23401 gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca gttagctaaa
    23461 cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg tgcgaatttg
    23521 atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt ggacaattta
    23581 catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg aaagatccgc
    23641 atggtacctg ttggcaaggg ctgtttagat ttccgcccgg tactgcatta tatcaaatat
    23701 gagcgtcctc taatgtacgc cactcttgaa gctactcctg agatttatgt tcctgaagca
    23761 atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg aggaacgaat
    23821 acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc atctttatca
    23881 gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac atcttccatc
    23941 ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga aagattcttc
    24001 tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg gtcaaaatat
    24061 attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag tagaagctgg
    24121 actaagagcg acccttatca agaaaatcca agaactatct attccttatc aaaaagaatt
    24181 gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc aaacgttgtc
    24241 ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag ctttaggtat
    24301 cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc ctctcgccgc
    24361 attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact tcaggcaaga
    24421 aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga ttgctcgagc
    24481 tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc aaatctatga
    24541 aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct gggctagttt
    24601 tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac aagggcgcat
    24661 ccaacccggc gatgtcgtga tgtatcagac gtatttcacg acgatcgtca attccgtttc
    24721 tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca cttctattgg
    24781 cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa tccaacattt
    24841 ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac tcaaaccgat
    24901 tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg ttgggccatc
    24961 tggctctgga aaatctacac tccttaactt attgattgga tttatccagc cgacttcagg
    25021 aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg tgcgaaatta
    25081 tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag agaacatcac
    25141 ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag ctgcgcaact
    25201 ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg aacatggcaa
    25261 caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga tacgtcagcc
    25321 aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag agaaaaaaat
    25381 ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg ctcatcggct
    25441 ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa tcgttgaaat
    25501 aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga aaaatgaatg
    25561 acaaaaaata ggagctatga caagactttt gtcgtagctc ctatttctga ataaacggtg
    25621 ttctcctgcc aactcctcgg tcacaagtca caatagtcat caaacatgag aagctgtttt
    25681 ttgaaattgt tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg
    25741 tgttcaagag acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg
    25801 tctctttcac accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac
    25861 tggtgtcatg atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg
    25921 catcaagtca ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat
    25981 tgcatcttcg tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat
    26041 cgcttggaag tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc
    26101 ttctttcaac gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct
    26161 atcgtagatg tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac
    26221 atggggttat tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag
    26281 ccgcttcacc aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt
    26341 cttttgcttg ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag
    26401 aatttcctgt gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat
    26461 ctgctacact aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga
    26521 agactttccc gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt
    26581 cgtaacccat ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat
    26641 ttgcttttgt ggcatcgaaa ttagtttctg tggttcctgt tttccctgct atcgtataac
    26701 ctgcaggatt cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca
    26761 tttcatcggc tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga
    26821 tcaccgctcc agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat
    26881 cattggcaaa tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc
    26941 caccaagggc taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg
    27001 ctttatcata tcctttttgt aaaccgatct catgcaaaag ccatacagta ggcaaattca
    27061 aactttgtgc aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat
    27121 gcgcatcgta ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt
    27181 ccagtgctgg tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct
    27241 gtgtagcaaa gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta
    27301 caccacctgt attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag
    27361 catttgctgg gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct
    27421 gatctaaagc tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat
    27481 actcgttaac tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat
    27541 ctgaattatc gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt
    27601 cttgttcttt tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat
    27661 cattcgcatt ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg
    27721 ccaaagtagc agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg
    27781 aagcgtcttg gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca
    27841 agatttcttc tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt
    27901 ttcgatcaaa cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg
    27961 tacttcctcc gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa
    28021 tccctttgat atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg
    28081 catcttggat atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca
    28141 gtgtgccagc ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt
    28201 ttaacgtttc gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga
    28261 cacagatcaa accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct
    28321 ttcgccatcg atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag
    28381 cagcttttat cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg
    28441 attcaatcgt cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt
    28501 caatatgttt ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa
    28561 gttttttctt aaatatgttt taaaaatggt acaattagtc tgcgattgca ctgaaaggag
    28621 tacacttgtg aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga
    28681 cttgcttgaa aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa
    28741 aaacgtttgg atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca
    28801 gatcacattg ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc
    28861 gaagcacatc cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg
    28921 aatgaagact catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc
    28981 ttatctcgac actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag
    29041 cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga
    29101 acaaaaaatg atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat
    29161 gaccatcacc aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga
    29221 taaaaatggt cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac
    29281 acaagtctat tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca
    29341 taagggacat ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat
    29401 gctccatgca ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa
    29461 agctttacct ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat
    29521 gataagactc tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct
    29581 aacagcaaac taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca
    29641 cttacttgag gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt
    29701 tgaaggagat gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac
    29761 gtcaacgtat agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga
    29821 tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct
    29881 gctaatcctt ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga
    29941 tggcgtttca cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca
    30001 ttgatcacga cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt
    30061 ccttgtgctg ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa
    30121 gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct
    30181 tttttgggag ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg
    30241 ccgaatgagc caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga
    30301 tccaatgttt cttctggtgt tgtggtaggg atacttaa
//
LOCUS       DACEDO010000031        29946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEDO010000031 DACEDO010000000
VERSION     DACEDO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.031"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..328)
                     /locus_tag="IWO52_001427"
     CDS             complement(26..328)
                     /locus_tag="IWO52_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0355703.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IWO52_001428"
     CDS             complement(353..2446)
                     /locus_tag="IWO52_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0355704.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IWO52_001429"
     CDS             complement(2935..3699)
                     /locus_tag="IWO52_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0355705.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IWO52_001430"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IWO52_001430"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0355706.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IWO52_001431"
     CDS             complement(4704..5780)
                     /locus_tag="IWO52_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0355707.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IWO52_001432"
     CDS             complement(5804..6397)
                     /locus_tag="IWO52_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0355708.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IWO52_001433"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IWO52_001433"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0355709.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IWO52_001434"
     CDS             complement(8067..8366)
                     /locus_tag="IWO52_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0355710.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IWO52_001435"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IWO52_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0355711.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IWO52_001436"
     CDS             complement(10121..11032)
                     /locus_tag="IWO52_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0355712.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IWO52_001437"
     CDS             11152..12009
                     /locus_tag="IWO52_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0355713.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IWO52_001438"
     CDS             complement(12196..12558)
                     /locus_tag="IWO52_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355714.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IWO52_001439"
     CDS             12904..14343
                     /locus_tag="IWO52_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0355715.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IWO52_001440"
     CDS             complement(14350..16902)
                     /locus_tag="IWO52_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0355716.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IWO52_001441"
     CDS             complement(17016..18347)
                     /locus_tag="IWO52_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0355717.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IWO52_001442"
     CDS             complement(18313..19791)
                     /locus_tag="IWO52_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0355718.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IWO52_001443"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IWO52_001443"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0355719.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IWO52_001444"
     CDS             22401..23828
                     /locus_tag="IWO52_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0355720.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IWO52_001445"
     CDS             complement(23920..24285)
                     /locus_tag="IWO52_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0355721.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IWO52_001446"
     CDS             complement(24282..24647)
                     /locus_tag="IWO52_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0355722.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IWO52_001447"
     CDS             complement(24839..25087)
                     /locus_tag="IWO52_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355723.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IWO52_001448"
     CDS             25086..25214
                     /locus_tag="IWO52_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0355724.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IWO52_001449"
     CDS             25271..26965
                     /locus_tag="IWO52_001449"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0355725.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27032..27238
                     /locus_tag="IWO52_001450"
     CDS             27032..27238
                     /locus_tag="IWO52_001450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355726.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            27616..28749
                     /locus_tag="IWO52_001451"
     CDS             27616..28749
                     /locus_tag="IWO52_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0355727.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28780..29100
                     /locus_tag="IWO52_001452"
     CDS             28780..29100
                     /locus_tag="IWO52_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355728.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29129..29593)
                     /locus_tag="IWO52_001453"
     CDS             complement(29129..29593)
                     /locus_tag="IWO52_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0355729.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29725..>29946)
                     /locus_tag="IWO52_001454"
     CDS             complement(29725..>29946)
                     /locus_tag="IWO52_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0355730.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         9031 a         6212 c         5170 g         9533 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
    27121 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
    27181 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
    27241 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
    27301 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
    27361 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
    27421 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
    27481 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
    27541 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
    27601 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
    27661 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
    27721 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
    27781 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
    27841 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
    27901 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
    27961 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
    28021 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
    28081 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
    28141 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
    28201 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
    28261 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
    28321 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
    28381 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
    28441 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
    28501 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
    28561 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
    28621 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
    28681 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
    28741 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
    28801 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
    28861 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
    28921 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
    28981 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
    29041 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
    29101 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
    29161 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
    29221 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
    29281 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
    29341 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
    29401 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
    29461 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
    29521 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
    29581 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
    29641 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
    29701 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
    29761 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
    29821 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
    29881 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
    29941 actaac
//
LOCUS       DACEDO010000032        29924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEDO010000032 DACEDO010000000
VERSION     DACEDO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.032"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2281)
                     /gene="esp"
                     /locus_tag="IWO52_001455"
     CDS             complement(<1..2281)
                     /gene="esp"
                     /locus_tag="IWO52_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0355731.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENP"
     gene            complement(2898..3332)
                     /locus_tag="IWO52_001456"
     CDS             complement(2898..3332)
                     /locus_tag="IWO52_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355732.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3349..4554)
                     /locus_tag="IWO52_001457"
     CDS             complement(3349..4554)
                     /locus_tag="IWO52_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0355733.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5248..5634
                     /locus_tag="IWO52_001458"
     CDS             5248..5634
                     /locus_tag="IWO52_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0355734.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5728..5919
                     /locus_tag="IWO52_001459"
     CDS             5728..5919
                     /locus_tag="IWO52_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0355735.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6433..6765
                     /locus_tag="IWO52_001460"
     CDS             6433..6765
                     /locus_tag="IWO52_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0355736.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7061..7216
                     /locus_tag="IWO52_001461"
     CDS             7061..7216
                     /locus_tag="IWO52_001461"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0355737.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7492..8832
                     /locus_tag="IWO52_001462"
                     /pseudo
     CDS             7492..8832
                     /locus_tag="IWO52_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9073..10689)
                     /locus_tag="IWO52_001463"
     CDS             complement(9073..10689)
                     /locus_tag="IWO52_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0355738.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10807..11025
                     /locus_tag="IWO52_001464"
     CDS             10807..11025
                     /locus_tag="IWO52_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0355739.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11220..11681
                     /locus_tag="IWO52_001465"
     CDS             11220..11681
                     /locus_tag="IWO52_001465"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0355740.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11874..12113)
                     /locus_tag="IWO52_001466"
     CDS             complement(11874..12113)
                     /locus_tag="IWO52_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355741.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12137..13660)
                     /locus_tag="IWO52_001467"
     CDS             complement(12137..13660)
                     /locus_tag="IWO52_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0355742.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13678..13800)
                     /locus_tag="IWO52_001468"
     CDS             complement(13678..13800)
                     /locus_tag="IWO52_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0355743.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13830..14813)
                     /locus_tag="IWO52_001469"
     CDS             complement(13830..14813)
                     /locus_tag="IWO52_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0355744.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14837..14986)
                     /gene="rpmG"
                     /locus_tag="IWO52_001470"
     CDS             complement(14837..14986)
                     /gene="rpmG"
                     /locus_tag="IWO52_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0355745.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15007..15384)
                     /locus_tag="IWO52_001471"
     CDS             complement(15007..15384)
                     /locus_tag="IWO52_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355746.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15416..15595)
                     /gene="rpmF"
                     /locus_tag="IWO52_001472"
     CDS             complement(15416..15595)
                     /gene="rpmF"
                     /locus_tag="IWO52_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0355747.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15610..15879)
                     /gene="rpsN"
                     /locus_tag="IWO52_001473"
     CDS             complement(15610..15879)
                     /gene="rpsN"
                     /locus_tag="IWO52_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0355748.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16402..18174)
                     /locus_tag="IWO52_001474"
     CDS             complement(16402..18174)
                     /locus_tag="IWO52_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355749.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18128..19882)
                     /locus_tag="IWO52_001475"
     CDS             complement(18128..19882)
                     /locus_tag="IWO52_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355750.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(19992..20561)
                     /locus_tag="IWO52_001476"
     CDS             complement(19992..20561)
                     /locus_tag="IWO52_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0355751.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20579..22003)
                     /locus_tag="IWO52_001477"
     CDS             complement(20579..22003)
                     /locus_tag="IWO52_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0355752.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(22005..22673)
                     /locus_tag="IWO52_001478"
     CDS             complement(22005..22673)
                     /locus_tag="IWO52_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0355753.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22803..23387)
                     /locus_tag="IWO52_001479"
     CDS             complement(22803..23387)
                     /locus_tag="IWO52_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0355754.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23718..23951)
                     /locus_tag="IWO52_001480"
     CDS             complement(23718..23951)
                     /locus_tag="IWO52_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0355755.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(23966..24916)
                     /locus_tag="IWO52_001481"
     CDS             complement(23966..24916)
                     /locus_tag="IWO52_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0355756.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24913..25755)
                     /locus_tag="IWO52_001482"
     CDS             complement(24913..25755)
                     /locus_tag="IWO52_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0355757.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25757..26476)
                     /locus_tag="IWO52_001483"
     CDS             complement(25757..26476)
                     /locus_tag="IWO52_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355758.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27168..27326
                     /locus_tag="IWO52_001484"
     CDS             27168..27326
                     /locus_tag="IWO52_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0355759.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27398..28624
                     /locus_tag="IWO52_001485"
     CDS             27398..28624
                     /locus_tag="IWO52_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0355760.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28712..29104)
                     /gene="rpsI"
                     /locus_tag="IWO52_001486"
     CDS             complement(28712..29104)
                     /gene="rpsI"
                     /locus_tag="IWO52_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0355761.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29120..29563)
                     /gene="rplM"
                     /locus_tag="IWO52_001487"
     CDS             complement(29120..29563)
                     /gene="rplM"
                     /locus_tag="IWO52_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0355762.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         9802 a         5524 c         4346 g        10252 t
ORIGIN      
        1 ctggattttc ataaatatct ttacttacag ttactgctaa atcggtcgtg cctacagaac
       61 catcttggta aacaatttta ataggaattg ttccagtaat cggctccgat tctcctacac
      121 ctttagctgg agtgaatgta acaataccag tgtttgcatc aacacttgca ttaccgaagt
      181 acttattata gacatctgaa attgaaaagg catctttagc taaaggaaca tctggtttat
      241 caaatgtagg ttttccactt tgaactgtcg tttctggagt tgttgttata ggtacgtatg
      301 ttgcatcatt ttccatagct tgagaagctt ttacagcaaa ctctacatcc acgtcgaaag
      361 ttcgatttcc gatcttaaca gttacaatac ctttcgtact atcagtaacg ttagaagtat
      421 caggctctgt tttccatgaa tactctgcat cttcaggtaa gtttttatta ttccgaattg
      481 attctttagc atctggttta aatccaacta ccacggtttg tttatcaacc gcttttggtg
      541 attccttaat aacggttgaa ccttcttctg gtttatcaaa acctggagaa acgatttgga
      601 tttttaattc atctttcgcg attaatttac ttgaatctac acccgtaaat tcaaattcta
      661 cagtatctga aattggagcc ccatcttttt catttggagc gatagttttt tctgctaata
      721 cagtaccttc tttatcaaca agttgggctt tgtgacctgt tcccgctaac tcgtggatga
      781 atactttccc cttaactgtt gtgtcaacat catatacttc ttctaattca ggtttcccga
      841 tatatggatt cgtgtctccg ctctcttctt tatcgtcagg gtttccatca ccatcaatat
      901 cttgcaaata ataataacct gttccataag tgttctggat taatttttta ttcgtatccg
      961 acaaccaaga atcaaagatg aaatcttcac caaatggttt attttcttca acttgtgctg
     1021 ttgctttcat atcttctaag atttcatcta atggcttagt caattgtaaa acatatcgaa
     1081 taacgactgg acgtggactt gcattagcaa aatcgataag tttagttaaa tcattattag
     1141 ttattgaacc tatgccataa ttttcatgac cttcccgtcg ctcgattgct aaatcagcta
     1201 cttttgttcc ggattcaaac gaaacatcat atgttacttt gtcagcctga accatgtgca
     1261 tctgtatacc cgctacatat ggaactaatc gttcatcaat tttgtaatta gcataccaag
     1321 gcgtgccctt tgcggaagat gaatatgaga aattataatt tttcgttaac ttatgatcta
     1381 agattatagc tccattagca cctgtggatc ctgaaccatc ttttgtttga gtattaggtt
     1441 tctcatattt tgcacttact gatccatgtt taaactggtc aggattattt tcagatacat
     1501 ctaagctatt atagaaatca tctgaatctg ttaaaaagta gcccgtacta tcaatacccg
     1561 gtacaatttt atcatcgtct tgatgactta ccaagtagat tcgatacatt aaacgatcgg
     1621 tcttgagatt gtctatgcct atctctttaa aaatttcatc taggctcttt tcaaacgtaa
     1681 ttaccccatt aggagcggtt tgcgtatcgg ttgtttctgc cccagcaaat aatccatcac
     1741 ttgcacgaat ataattgacc ttccaaatat ttgtaggtct acccagcgaa tcattaattc
     1801 ttacaaaggt ccgtctagaa gatccaattc ctttctttgg atcgacctga attttttcta
     1861 catatttagc taatcgttca tctaattgaa gacggatttc ccagttttta gttgatgctg
     1921 caatattatg ttttccgtaa acttcaaatc caattgtatt gccttttagt gtgtctccag
     1981 ttagttcttg aggactaaat tgtaaatccg aaaagttata atttttcgta taaccaggat
     2041 tctcctgaaa agtattcccc gaagctactt tgtcacctac ttgtacggag cttatcgctg
     2101 tttcaagtgg gctgcttgga gaaatttcat cttgtgcttt aactagttca gttgttccca
     2161 agacaagacc aacaccaatc aaaacagatg caaccccaac acttaatttt ttgatactgt
     2221 acttcaatac tcgtttcttt gttttttcta gaaatactct cttattattc ttgctaacca
     2281 ttattcaaat caactccctt aataattact ttcctagtaa gaataaaaga taaatctgat
     2341 acgtattatt cacagaatag gaatcttact aaccttatgt taactaattt acttagagaa
     2401 tttcatgaac attacaacaa aaaaagtaca tttcttcatg tttttttcgg atatttgtgc
     2461 tttttaacca ttcagcagct gaaaacatat aaaaagatat gtatgtattc tttctgaaat
     2521 taagttcatt tctatgcgat gaagcatgtt ttcaaaatat taccctttct tagattaaac
     2581 ttcagagtat tcaaataacg gaataaggag ccttatcccg cattactgaa tgagactgtg
     2641 acataagtaa aaaggtgact tcaaattcct tcttcggaat ttgaaatcac cttctttata
     2701 attataaagt atcggtttca cagcatttct gaatgattcc tagaatctta aacgaatgat
     2761 tggagttatc aacatttttt cactcgtttt tagcgaaaca aaaaatgaca tttgtcacag
     2821 cctcattaaa ttattgattc tttttcttta taattgaatt gtccatattt aaagtgtcta
     2881 gtactctaca tgaataatta attgctttca ctcaaatact tataatctat taacttgtag
     2941 ttgtttaact tttctgaaaa agttagaccc acgtaataat cttcccagct ttctctttca
     3001 attaactgat ccaatttttg ataatccata aattcatcat gtatcaaaaa gaataaataa
     3061 cttgatattt tagcatctag cttaatcaga ttaaataatg tttgtttaaa attgctacca
     3121 ttaatatgac tgatagattc tctagcttct tcacataaac tttttacttt attaattact
     3181 actttgtcat attcatttaa cacactatta ttaccattac ttagacgctc agattctacg
     3241 aaacaactca aatcagaaat ttcccaaaaa aattttttgt taaaatccaa tttcctcaac
     3301 tcctccaaac tacgtaaatg atatagtctc ataataaaat aaaacctctc agctaatgtt
     3361 gttgaaattg tccactaaac ctaattgata ttgcttagga gatacacctg cgtacttttt
     3421 gaatactctc gaaaagtgtg aagaatctga aaatcctaaa cctaaagaaa cttcactgat
     3481 agtatggttt gatttcaaca ttattttagc cgtagaaatt tttcgcttag agatataatt
     3541 ataaatcgta atatgcattt cattcttaaa gctttctctt aatttagttt cacttacaga
     3601 aaaataatta gctatttcct ttacagtaat tcttttatag atatttaagc cgatgaattg
     3661 aacgacagaa gaaattaagg gagaaagatg ctctatctta gtttttccga tttccttagt
     3721 ataataaaca atcccagaat ccctcacttt taagacctca ggtaacgata cagctaattc
     3781 gttttcttgt atcaaagcat ctctactttg atacgcttct attatatcca ttcctaatgt
     3841 tatagacgtt tgtgctaact tttcaaaaac aatgatagaa taattttttt cagaccgaat
     3901 cgtatctcca ctaacacttg gaattattcc attactcaga ttaaaaacca tgttttctaa
     3961 atttcgaata tctccatttt taacatattc cagtatttta ttttcataaa gaaacagata
     4021 tatttctgga tcgtaatttt gtgataacag attatctatt ttgatttgct cgatagtttt
     4081 acttagattt tctgtatatt caattaacgg ttcatggaat agatcttcta tttttccagt
     4141 aaaaaaataa tgaactaaaa tcagaatatc tcgaatgtct cctaaagaat acaaagtcaa
     4201 attttgcaat gaattataga gtcgttctct atcagccatt gcaacattca aattgttcat
     4261 ttttagttta aaaaaaactt tatcaatatt atttgctcta aatggaccaa ataagtaata
     4321 tctggtttta aacatgtata gaagaaacat ctcattaaaa tcaccagtaa agaaatggaa
     4381 atctttagtt tttttaatat tatcagttag taacttttta taatcataaa aaaaataagt
     4441 agttctatct gagcgatact cttttataat actgaattcg gaattaaaaa ccacgatagg
     4501 taactttaaa caaacatgaa gagactttaa taagacttct ttatctgaaa acacttttgt
     4561 ttctctcttt cctttctaca tcattaaata gtattttata cgttatctaa aatactattc
     4621 ctctgtcaaa ataagctaac ttacttcatt ataaaatatc cacttatcgc tttcatgtat
     4681 attccaccgt aaaaagtaca ttttatcacc gagaactcat catttttcat catgatgaat
     4741 actatttaca tatatctagt tcaaagacaa taattattat gatccgttat tatctaagca
     4801 ctatacagat aataggaatt tttcgtttct aaatattcca atttgggaat ccaataaaaa
     4861 ttttcgttca aatacgtgtc tattcgaact cttttctagt tgttaattat gaagggggaa
     4921 gaaataaaaa atacaatcta tacacgaata agaatattat ccaaaaggag gttttagaaa
     4981 agatttatta tacgtaaaaa gctcaagaca ttttcttagc cataaggaca tgtttgatat
     5041 ttaagaagat atatgaattt tcttttctaa gtataaagat aactatccat tatcaactaa
     5101 aaaaacattt ttgaaaataa acatcagcag tcctatcaat gcatgtgagc ttatagaagt
     5161 tattcgcagc gcagcataat tgcattagct tcttttcgtg ttttttgcct cttttccctt
     5221 taaatcttaa tggaggaggt gtgcaatatg tcagataacg acgaacgcgt aaatgcccag
     5281 ttgattcgat tctttgaaaa ggtcattttg aacaccgcta atacttatta taaaaaacaa
     5341 gaagaaattc aaaaacatga acaacttgat caactcccac catatttgtt tactaaagat
     5401 actataaaat ttaaacaacc gattaatgtt ttaaatgtga ctctccttgt tgaagatata
     5461 aaattagcaa aaatccttcc tttgttgaaa gaacctgaac aaatttttct tatcgaaaaa
     5521 tttatctttg ataaaactga taaagaaatt ggaaaggttc tggggattac aagacagggt
     5581 gcgacaaatt taaaacatcg actttacaaa aaaattcaaa aaatgctggt ttaatatctg
     5641 cataataact aattttgtat gcttctttgc tcgttctcct ccttcccaaa gaaatagatt
     5701 ataagtccat gggaagaagg tagatttatg aattctttaa aagaagattt cattttagca
     5761 aaggctggaa atgaagaagc tgttgaggca attctcaaaa gatttagctc tttgattcat
     5821 aagcaatcct ggagaactgg aaaatatgat caagattgtt atcaagaatg tatgcttgca
     5881 atatatctag ctatctcgaa attcgaaatt aaagaataac tcaactaagg gagtctgtga
     5941 cataagtaaa aagatagttt caaatttcga acaataaatt tgaaactatc ttctttataa
     6001 ttataaagta tcgattttac agcgttctat aatgattcat agaatctaaa acgaatgatt
     6061 ttaattatca acgtttttta attcgttttt agcttaaaaa taacttttgt cacagtctca
     6121 ctcctaaaat tactttattt acgtatgaag tgggcaggag gtcgaatcca gaactgggct
     6181 atggtacttc aacgaggact tctcctctag aattgaaaag catgcgattt accttccgta
     6241 acgacggtag atttattcaa ctatgtgtcc aatcaaaatt tgtctgattt caaaaatagt
     6301 caagagaatt ttggttgtta aaaactctta tctatcaact tccacagttt agttgacaaa
     6361 ccctgatact aagttgggct atgatacact tttcttgact atcagttacg ttaccggaag
     6421 gaatgaactc tattgagaaa aacatatgat cgtaaattta aactaaagat ttcacaggat
     6481 atccttgaga aaaaagtaac taccaagaaa attgctgaag aatacaatat ctctcgtcca
     6541 acaatttccc gatgggtttc agaatatcgt cgatacggga aaaatgcttt tgctggacaa
     6601 ggaaaaagat tacctgataa agcagatttc tacattcttg aacaggaaaa taaacgacta
     6661 aaagaggaag acgagtgttc tacgaacaca aagatcgata cggatctcca gggatcactc
     6721 aaaaacttta cgatgaagga atcgaaatca ataaacgtgt tgtagctgtt cttatgcgtg
     6781 aaatgaattt atgcacaaga ggatttcatc gacgcaaatc ttctcatggt agagaaaaaa
     6841 caatcaaaga aatggtaaaa aaatgttgaa tcgataattt gaacagaacc aaattgatgc
     6901 tgtttgggtg atggatatta cttacattcc atgtaatgac ggtcgcctct atttgtccac
     6961 ctatattgac ttagcaactc gtattctacg ttactatatg gtagattcac acatgaaaaa
     7021 agagattgtc gtccgtccat tacaaattta ttaaggagaa ttgcctacgg taattcattc
     7081 cgatagaggt agccagcatc ggtcttttat ttatcaagag ctgttgaacg accaccacat
     7141 cacacattca gtgagccaaa ctgggactcc tgttgacaat gcagtaattg aagcgtttca
     7201 tcgatcaatc aaatgagaat taatcgactc caacaaatat aaaagtaaaa tagagatgaa
     7261 ggtgttaatc caagattatt tagaagacta ctacccaaac aaaagaatcc atacaaaatt
     7321 tatgatgact ccttgacaat ttgaagcgaa gaaaataagg gatctataat atctagtgtt
     7381 ggtgacgcct tctgttgcca atactttttc ttttttctaa taaacgcaaa cagacacctt
     7441 aaaatccttt agaataaagt cgacgaaaac cattcaaagg agtgtttcaa gatgtcctat
     7501 tcagagttta ccaaagagtt actagatatt ctagacctaa atcttatctt tcatgaagat
     7561 gcctttcgca aagagcgaat caatgacgaa acatgttttg tttttgatgg cacattgacc
     7621 tatcaaccgg aggaatgctt tcatcgccac tatcaaaatg aacaaacgat cattaaatag
     7681 ggctggaaaa aagtatccat cttattgaat gatgtcagta actacaagac cattctgcgg
     7741 attaacaaac agcgcttcaa atgtaaacac tgtggaaaga cttttttagc agaagattca
     7801 gttagtgatc gtcgctgctc cattgctcgc agagtcaaac aagccattct tgagctatta
     7861 agcgaacctc tttctatgtc tttgatcgca cgtatgaagc acatttctcc gaccactgtc
     7921 attcgaatac ttcgcagcct tcggcctaaa accatttctt tgagccagct gttaccagaa
     7981 gtggtgtgtt tcgatgaatt caaatcagtg aaaaatgtct cgggagccat gagctttgtc
     8041 atgatggacg gcaagaccca tcaactgatt gatattgtgg aaaatcggca actcaatcct
     8101 ttacgcgact atttcttgcg ctaccctata aaagtccgtg aacaagtgcg tctagtggtt
     8161 tcagatttct atacacccta tcgcacattg gtgaaagagt gttttccgaa tgcccgaatc
     8221 gtcgccgatc gctttcatat tagccaacac attggtcggg cttttaccaa ccaccgaatt
     8281 caagtaatga agacctttaa aaaaggggat cgtcggtcga agcacttaaa gaaatactgg
     8341 aaactacttc aaaagaatgc gtgggaattg aaaggtcagc atcgctactg gcgtccgtca
     8401 tttcgagatc atctgacaga agcagagata gtcgatcggc tattatctta tgatgattcc
     8461 ttaaagcgag gctacgaagt ctaccaggat tttctttccg ttatcagaag acaagatgtt
     8521 cctgaattcg tcgcgctcct aaaagaagac tacaaggagt tgccagagca ctaccaacca
     8581 gtgttcacga ccttcaagaa ataccaaaca gaaatcaaac gagcgttgcg cgttccctat
     8641 tccaacggcc ctatagaatg tttgaataac catatcaaag tactaaaacg aattgcctat
     8701 ggctatagaa actttcaaaa ctaccgagaa cgaatctttt tatatcgagg aaaatatttt
     8761 aagaaaacta agaatactac ccaactgact aaagccagaa caacaacacg gctagacaag
     8821 atagctgtct agccgtgtga gtatctcgat tattctattg aatttcttca ccaacactca
     8881 ttgacgaaga gccaaaataa gtaatctaac cgactgttaa ctaaaccatt gcaggtcccg
     8941 ttaaagatac ttgtgcctgt tgtcaccgct tctttttctt gtccacaagc gcttaacaca
     9001 cttaataaag cccctgctat tattaataat aatatctttt tcttattaaa aacctcctca
     9061 tctatttcca gattagtaaa tgtagtaatc tgtgctttct tttttctcaa tactaataac
     9121 taacttatgg ggtaaacaaa tactcgtttg accgggtttt gagatccatc cagttctaac
     9181 cgcgatttga tcaggactat tatcttcttt aactcgaatt cttccatctt taatctcaac
     9241 aatattatat tgtcccttcg caggatgata attcacgagt ttatgattga tcttatccaa
     9301 atcgaagcga tccatttcct tgccatcaat tcgaacgatg gcatatttga tagcatcttc
     9361 agttgtttca ttctcctttt ctatagagaa tacaaataag ggaataaaag aggtaataat
     9421 caaaatgcca ataattatga catcgcctat cttgacacca gcttttctta aaaatttcat
     9481 taacgatatc tggctaaaca tattagactt tttccatgga aatggtgaaa tttttcggaa
     9541 attatctgca ttaagagaat cagttaaaca accctcaagt tgttccttaa tacattgttg
     9601 atccatcttc ataccaataa aaactagttt attaatgcgt tcaccaaaca acggatgcca
     9661 ggcttccttc aattcgggat ttttttgtat aatcatttga cactcaattt taggtaaact
     9721 agctacccaa taatttaatg attcaatact tttatagcta cccgatgttt caaagatcgt
     9781 cgaaaaatca ctatgttggg caaaccaaag aaaccctttt gctcttgtaa tattgccagg
     9841 aaaattttta tccgtaagat gggcaaattt ttgtgcgtca aggagtaacc gatttcgata
     9901 gataaaactt gagataccat attcttctgt ttcaggaata tgattttgat agccaagctc
     9961 taactctttg aaccagccca ctgaatagga agtctcatct atattaaaaa gatttgtatt
    10021 tagaattttc gataagtcta cttcaccttt aattgtccta attattttgg cattggactg
    10081 taacttagca attagaccgt taattttgta aatcgtcttg gttgacacta agtcactatt
    10141 atttaaaagt aatatgttac aacactcgaa ttgatcaatt aacaagccac gaatatcctg
    10201 ttcttcttca actacattac cttcagcttt tctttcctga atcttttcac ccgtttcgaa
    10261 attatcccaa aaacgtttta catccactac tataataatg gcaccaatct ctaatcgttg
    10321 ggtcaaatca atgtctaaag ggtttttagc taatataatt gcttgaataa ttggaattgg
    10381 tttgctgatt cctgaagctt caataacaat ttgattcaag tcagaaaatt gcactaattg
    10441 atctagttca acaatcaaat cttcgcgtaa tgtacaacaa atgcatccat tcggcatcga
    10501 aacaagtttt tctccagttc ttttaaaacc tttcttgtcg actaataccc catcaacatt
    10561 tacttcccct atgtcattta caatgacagc aatttccttt ccgtgatcac cgtacagaat
    10621 atgatttaga agtatcgttt ttcctgaacc aagatagcca gttaagattg taataggtat
    10681 ttttttcata gggaacctct ttctatacct tgaatttaca ctttaagtgc aacacataaa
    10741 aatgagtcat aatcgatatc caactagtat aagtttgtcg agaacactcc tagaaggaaa
    10801 ataattatga gccagtatcg tcatcttact atactcgaac gagaaagaat ctttctgctt
    10861 cacgaagaag gattttctat tagtataatt ggcaacgaca ttcagcatag cccgtttact
    10921 attttacatg aactgagaag aaacaagaaa aaaactattc accttccaaa gcggaaaaaa
    10981 attatgctta tcgaagaaaa aaatgcggac gttatcacat tctaaatgat ccgtgagttt
    11041 ggtcaattgt tcgtcattta tttgtcgatt ttcagtggtc gccagaggaa atctcgaatc
    11101 gtttgaagca tgaacatatg gacttttcaa ttagttttat cacgatctaa cgtgcaattt
    11161 gcaatgatct tttcgataca gaacccctct cacacggcaa taaaggcctg atccgttcat
    11221 tgcaccatcg aggaaaaaca agacatacaa aaaatcatgt cgagtctcgt ggaaagattt
    11281 gccttactca ttcgatccat ggccgtccaa aatccgcaga tgatagaagt gattttggtc
    11341 attggaaagc cgatattgta gctggcaaaa ctggtggtac ttgtttagtg actttgacag
    11401 acagaaaatc atgcttcctt ttagcaggaa agaaggtccc cagaaagaaa gccgcttatg
    11461 tgagcgagaa aatgattgaa cttttaagca gtattccaaa tgaggcggtc aaaacgatca
    11521 ctccagatag aggaaaagaa ttctccaatc atttcgcagt tacagaagca ttgaataata
    11581 tgccctttta ttttccagat cctcatgccc ttggcaacaa tgaacaaatt aaaatacgaa
    11641 tagactcatt cgagaatact tgccaaaatc gaaagagata gctacggtag ataactcaac
    11701 gataaatcag atgattgaaa aactgaataa cagacctaga aagtgtttag attggagaac
    11761 accttatgaa gtcttctttg ataaagtgtt gcgcttgatt tgacaattca agacataata
    11821 aaaagcagat caaatccaaa taagggaaaa atgatctact ctggtattat caattaattg
    11881 gccaagctat tatgccgttt tgctaaacgt ctacttcttc cagtattgac cttgataaaa
    11941 caacaatcag gatcccgttt taatactcta tgaactgtgt caatactaac atgtaaagtt
    12001 gcagcaatat cagctaatgt actcccattt aatctcattt gctttgcaaa tcgataacgc
    12061 cgataagcat caatattctt ggaatccata gacaataata cttttcttac catgtatttc
    12121 ctccaaaaat tgaattttaa tgagccatca tttcttgagc aatttcatgt ttagacaatg
    12181 aagctggata gaaagtaggc caattatgca attcattata gagtttttct tgactttctc
    12241 caccaaagaa aatatggaaa tgctctgcct tacttggtgc aattccatga tcactaaatt
    12301 gaatatattt aaaacgacca gcacctgcat catcagtttc aaaattaaaa cgaacaccac
    12361 ggtttccttt ttcataattc aaaattttgt atccaacata tttgtatgtg tattgatgat
    12421 gttcaccgtt gactacaaaa tcaatggtgt tgtctttaat gttgatagta tcaatatctg
    12481 tcttatagcc agtagtataa taatctttgt actcggcagc tgtcatgtct ttattgattt
    12541 ttgctttgta atcgaaaact tcatccaaaa ttccatcttt taacaacgga taaacagatt
    12601 gccattcgcc tgtataatca ctcaattcgc gatccttcac atcggcatct ttaaagtacc
    12661 cattagcaac tgtcttttca tccttcgctt ctttttctgg aactacctct tcaccagcaa
    12721 ccatagttgt tttttctaac gcttttagat tgtcttccat tactgaaaca taatcttcac
    12781 cattatccat ttgttctttt gtaagacttt caagaggatt cagtacttcc aattttatcc
    12841 cagcttcgtt ggccaaagtt tttgcaattt tatcattagc attcttttca aagtaaatat
    12901 aattgatctt atttttttta acatactctt ttaattcagc taatcgacca gaagatggct
    12961 cttcttcagg acttagtcca gcaataggaa cttgtttcaa tccataatcc aaagctaaat
    13021 atccaaaggc tgcgtgttgc gtcacaaaac ttttttgttt cgcttcttta agactagtgg
    13081 tatagtcagc atccaaccgt ttcagtttag tcaaatactt ttcagcattt gtctcaaata
    13141 cttttgcttt tttcggatac aatttaacta actgatcctt gatatttgaa acttccttaa
    13201 ttgccatttt gggtgaaacc cacgtatgag gatctagttc atgatggtga tcttcttctc
    13261 cgtgatcatg atcttcttct tcactacctg gcaataaaat catgtctttt gttccttcta
    13321 ctacattagg cccagctttc ttccaacttt ctttagcttt tggtacccat gattccatgt
    13381 tttcgttatg atatacaaaa acatcagcat catgagcttt tgccatatcc ttagctgagg
    13441 gctcatagtc atgaggttct gatcccgctg gaaccattaa atccacgttc gcttcatcgc
    13501 cgacgatgtt tttggtgaaa tcatacattg gatagaatgt tgttacaatt tctaattttc
    13561 catctttatc attcgatact tttgcatcat tagtgctgga gcaagctccc aataatccta
    13621 gagctgctat cccaataagt cctacactga ttttcttcat tctttttcct tccttaatta
    13681 tgctaatttc ttcattttat ttcgtttatg ttctttgata atttttaatg ccctttttct
    13741 agttttaata ttcgggcttt taagtcgtcg atatgcacta gatatatccg ttttctccat
    13801 tagaaattac ccccaatcac tacttatcat tatttgcttt ccgccagtaa tttatcaaaa
    13861 ctctgtttga tcctttcttc atccaacatt tttccgatta gaataatagt ggtttggtct
    13921 ttttgtcgag tggtgtattg aaaattaacc tgttcgttta cctcttgcat ggcaactata
    13981 aaatcctgct cttgtaaagc aatcaatcct ttaaaacgat acaggctccc ttggttggtt
    14041 aaaatcagcc agtcaatcca ttggagtaaa aaatcttttt ttatgtcttg atctgaagtt
    14101 aagagaattg atttaaactc atgtacttga tggtgttgat gatagtattt gccattatag
    14161 tgattttctg atgaatcact ttcaaaaact aagggatgat taaatttttt caaattataa
    14221 aaatcctttt tttgaatcgt ttcactatgt gtgaacattc gaatatcgga taaagggtta
    14281 atacttttga tttctctctg taattttttt agtgcagata aattcaagtt ttgttttcta
    14341 gacacaaaaa tgcgatctgc catgacaagt tgcttaaggg cttgtggttc tctttcaata
    14401 agttcaaaac tgtcactatc aataacagtc aataaactat cgatatatac tcttcctctt
    14461 agttgaggcg ctgttaaaag tgtttgaatg attggttggg gatctgctat acctgtagtt
    14521 tcaatgatga tttgtgaaat tggttgttga tgttcttcta ttgtttccag taaagaaatc
    14581 aatattgcta ataaatcact gcgaagactg caacacatac atccattatt tagttgtaac
    14641 acccgttcct cgctatgaac taataatttg tgatcaatcc caacttcacc aaactcattt
    14701 tcaattataa taatttcttc cctggggtag ggtgagactt gcaaggcttg attgattagc
    14761 gttgtttttc cagcccctaa aaacccagaa acgattgtaa ctggtatgcc cattttgtca
    14821 cctttttacc acttagctat ttcacttctt taaaaattgc ccgtctgcgc aattttggcg
    14881 aatacttttt taattctaac cgttcagggt tattccgttt atttttgctg gtgagataca
    14941 gacgttctcc agtttcgaca cattccaaaa taatattttg acgcataaat cttcctcctt
    15001 caatacttat cgatcttcaa gatatgcttg aatttgttgt gcatcttcaa aagttaactc
    15061 gccagattcc aattgtgaaa ctaaattttc tttcatatca acaggtatct cccaataacc
    15121 atcgatacat tgaaataact cctcgtcata ctcttcaaaa attggataga ttcttaaaaa
    15181 atcatcaaat gataagcctt ggtcctcata gtaatctttg actaatccat tttcaattgc
    15241 ctgttgggtc ggtaaacccg tttcagtaat aattcctctc tctaccatcg aacgataaaa
    15301 agtgcattta tctagcgatt gctgggtcgt ttcaagataa tactttaatc cttcttcaac
    15361 taaaggtggg taaataattt ccattttgat tccctcctaa aagttatata ttaaactatt
    15421 ttgaagctcc tttaattact ttctttccat tatagtaccc ttttaaagat acatgatggc
    15481 tacgtctata atcaccattc gcttcgtcaa atgaaatttc tggttttact acagtttgat
    15541 gagttcttct taaccgtttc ttcgccttag atgtttttct tgctggtact gccatgatat
    15601 ttcctccttt taccaacttg ctttttttac tccaggaatc aacccttgat gggcaagttc
    15661 tctaaactta atacgggaca taccgaactt tcgcatatac cctcttggac gaccatccgt
    15721 ctgatcacga agcctcagac gattcggatt tgaatctctt ggcaatttag cgagagcggt
    15781 gcgatctcct gcggctttta attcctgacg tttatctgca tacttttcaa tcatcgccat
    15841 ttgtttcttt gctttagcaa ttttcgactt ttttgccata tacctttata ctccttttat
    15901 tttgatttag acacctcaaa tcgtaatcgt tacgttttga cactgatact ttaccaacaa
    15961 gagttgctct tgtcaacaat aaataacatg aaataaaact catatgaata gataactatt
    16021 tgaaaacgaa tttatcggta catagagata atcaaatcat ttaaaagaat atgcagttta
    16081 ggctctgaaa aataagaatt cacttttatt agtccaatca aacacaaata ccgttttaag
    16141 tagcttttcc tctacttaaa acggcaatat gaaagaaact ataacttttt attcaatctt
    16201 ttatgtttaa ctggtttatc gccaattttt taaaactttg attattcctt atattataag
    16261 aatagacatt aagtgaacta attcaatcat actgattctt tcgtgtattc aaaattagat
    16321 gctgtttaat aaaattattc tttttgaaaa agaattataa ctatatccca aaaattgtag
    16381 gttaatttaa agcctatttc atcatcgtga ttgagcttct ttttcataga cgaatttctc
    16441 agatagttta gttttcttta tgagattttt gtatatttct gaccttcgat acaactcatt
    16501 atgagtacct tgatcaacta gttttccttg atccatcact acaattttat ttaccttttc
    16561 aatagacttc aagcgatgcg atataattag tactgtctta tcttgtatta aacgatttaa
    16621 actttcctga atacgttttt cattttccgc atctaaactg gcagtaattt catctaaaat
    16681 taaaattggc gcatttttca aaaaagcccg tgctatggat aggcgttgtc gctctcctcc
    16741 agaaagtgaa cttccgtttt ctccgaccaa tgtatgataa ccgtcaggta gtttttcaat
    16801 aaagtcttcg cagttagcta aacgagctgc ttttttcact tcctcatctg acgcttgact
    16861 atttccaata cgtatatttt ccaaaatact tgtattaaac aaagttacct cttgaaaaac
    16921 aatagcgatt ttgctaaaca aagaagctgg agatacacgt ttaatatcat acccatcaat
    16981 taaaatacag ccttcatcat aatcatacag tctagacact aatttcaaga tactagtttt
    17041 cccggaacca ctagcaccta ctaacgcagt cacttctcct tgtttagccg taaaagagat
    17101 atgatctaaa attggggtat tgttatcata tgagaaagag acatctcgca actcaatgtc
    17161 aaaagatttc agaggactat ctgatccctc ttggatactg gtctccttca tagctctaat
    17221 tcgctgtatt ttaggagcta aataaaatat ttcaagcact gcttctttca tagaatcgaa
    17281 ggaatccttt atttttattg cggctagtaa gtaaccaacg acgtagagta tagtcacttc
    17341 acccgttaac agtaagtgaa cacctactag aatgactact gctaaactaa catatgagaa
    17401 aatagaggat aaggccatga ctgaaaaagt acttaattct acctttaaat gaatcctttc
    17461 actctcttcc atttttttga aaagtctgtc ttgaactttt ttagatagat taaagctatt
    17521 aatctcttgc tgcaattcta tagtttcttg aaattcttcc gagttttctc tcaaagtatc
    17581 gtaatattta gtattggctt tcgtttggga tttctttgat aacaagatta agacaaaact
    17641 aaataacgtt ggcaaaataa cagctaatcc cattttgaca ttaccaacca aaagcatcac
    17701 tgaaataaaa gggaaaaaca gagccatacc gccggcttta ggtattgcat gactcatcgc
    17761 atgctcaata ccttcaacgt cagacatgat agtttgagat aaatctgata aattatgttt
    17821 tgaaaaatag gataatggta aatttgacaa atcgtctgca atttgcactc ttaaatgcgc
    17881 actttcttta taagtactat tatataagtt ttcatactcc ctatctaaca accaatacat
    17941 gaaaagcaaa gtaacaaacg atacggcaaa ataaagccaa cggggctttg tattttctaa
    18001 aactaattcg tctatcagca tcattataat gaatgctggt accatattaa tgcagtatac
    18061 aaaaaaacta gaaatagaag ctttagttaa agcttctgcg ccctgatcac ttaaagcaaa
    18121 tttgttttta taaaaacttt tcattgtcaa gcctccaatc atttgcattc tcataattag
    18181 tgaccaattg tttatacact tgatgtttag ccattaattc attatgattt ccacgttcta
    18241 ctattttccc ttctgacaag acaagaattt catctacaga tttaatgctg gataatcgat
    18301 gagctatcat aataactgtt ttttctttta tcagatgttg aaaagccttt tgtagttcat
    18361 actcattatc tggatcaata gatgcactgg cttcatccat aataacaatc tttgcatctt
    18421 ttaaaattgc tctggcaata gctatacgtt gtttttctcc tcctgacaga taaactcctt
    18481 tactaccaat taaggtatgc tctctttcgg gaaatttttc taaaatcgat tcgcaaccgg
    18541 ctaatcttag cgcgtcaaat actttacttg ctggcgcttg aggatttgct atagaaacgt
    18601 tatcgtaaat actctgattg aatagtttcg tatcttgaaa gacaaaagca attgtgttaa
    18661 taattgtgtc ttttgaatag tgatttaaag gcttaccacc aattttaatc tgtcccttat
    18721 ccacattgta gaaacctgaa attagtttag ctatcgtact ctttccgctt cctgattttc
    18781 ctactaatgc ataaattctt ttttgttcta aaactaagtt gaaattttct aaaacacact
    18841 cttctttgta tgaaaagctg acattttcaa atgcaatgtc acaattttta atctgtgtct
    18901 cgttcccata ttttagttgg tcttgcttca tttctaaata aagggagtct agtgtttcga
    18961 ctgcatatct tgcttcaaac aagtgcatac ttacatacat tattctcatg aaagaaacaa
    19021 aaataattcc acttaaaaaa aacaacatca acaagtcaac agctaatatc ttgggagtgg
    19081 taagagaagt tataaaaaag gttataggaa ctatcaaaat gcaaactatg ccaaaaaata
    19141 gccattgaaa aagaacaaaa ggtgttttcc cactcaaaga atacttatat gcaaacttag
    19201 cataatcgtt aattgcttta ttcaaggctt taaatgatgt tactttagca ccaaatatct
    19261 ttataacttg aattccgcgg atatattcta ccgtttcaga gcttaacaca tctaacgatt
    19321 cttgatattt ttgaataaaa tttgtattct ctgtcatttt ctttaacaag aatacaccaa
    19381 ttactaacat ggcgattaga accaatccaa ttttaaagtt aatgaaaaag cctaaggcaa
    19441 ctgataaaac aggtgttact atggcttgag cactatctgg aatcaagtga gcaattgcca
    19501 tatgtgtttt agccgcgttg tcatcaattg tctttcgaat atatcccgaa gaatttaaat
    19561 caaaaaaacg aaaacttgct tctgttaaac cgctaattcc cttttttctc aaattagtct
    19621 ctaagcgaaa cccaaggata tgagaaaata agccagaaaa aatataaaat aaagctccta
    19681 aagttaacca agcaacagtt tgaaccgcat aactcttagc taaatattca tctcctacaa
    19741 taattagaga ctgaagaaat ttgtaaatac tgtaatatcc atagactgtt aatacgacag
    19801 aaaaatcaga aaataaagtg gctaaccatc cgtagtattt cattttaggg acataactaa
    19861 ataatttttt gtatactttc attttttcct cccccgcatt caatttcttc tcttattcgt
    19921 ttacaactgt taatgctttc cgtaatagcg taaattagag atgtacaaac agataaaagg
    19981 taaactacgt tctatttttt gtattttttt aagacttttc ttccaatcaa tacaccaatc
    20041 atagataaac ttgctacaac taacactgaa atgattcccc atatacctac cgcatagtgg
    20101 atcattgcat ccattgttgc agcagattgt cctttatcta tccaatcttg acgaaaacga
    20161 tctgtaaaga accatatagg aatccactgt ccggcagcac tgcctaattg cattagagcc
    20221 caagacgcaa cttgaggaat tgctcgatca tatccaaata cagatagaat aatatcagct
    20281 aagatcgcca tactaatcga actgataaac caaggtaagt acgccccgcc cattaacgta
    20341 aaaaataaca ataaaacaat tgtgtaaata gtaaaaattc cttttctagg gtagttatag
    20401 ctaatataaa caaatatgat tccagataat aatccaaaaa tcccggggga tatgctgtga
    20461 ccaaacgccc caccaatact actaaccatt attgaaaaaa agtaaattac tagggaaata
    20521 actgtagcta aaccaataaa aatataatct tttgttttca tatatattct cctcaattct
    20581 atttcgttat gtcattaaaa atctgttcta tcaaagttga atcttctact ctaagtttta
    20641 tatctttttt tattccacca ttttttaaat aaataattct attgcatgtt ttataaagaa
    20701 attccggatc atgagtagct ataactactg cacccttttc ttttaagtct ttaataaggt
    20761 cagcaatcct ttccatatta atataatcca acccacttgt aggttcatca aatagaaata
    20821 aattagtttt tgacaatgaa gctatagaaa ccaataatct ttgtttttct ccaccggata
    20881 aatcaaacgg taattgatct ttcattttat aaagttccat tttttttaaa attttttctt
    20941 gctgatcttt actaactatt tcattcccca gggctaactc gctcctcact gattctgtga
    21001 aaaattggta atccatttct tgcattacta aaaacggcga tgacaaattt tttttatttt
    21061 cgattatctt tcctttggta gggcgaataa tcttcatgag tattcttagt aaggttgttt
    21121 tccccacacc gtttttccct accaaaccaa taacatctcc aactttgaca tcaaaattta
    21181 tgtcttttag aatattttca taagtaattt cttcaacttt taatacatct gcacttacct
    21241 tttcgatata gggaacttca atcgaactat taaaaatgtc aaatttcctt aatgcgttat
    21301 tgttttcagt ttttaacaac tgaatagttg tagtttttac agttctattt ttaacaaata
    21361 gaacttgatc aattaattct ttcaaatagt aaaaacgatg ttcagctata acaatagtaa
    21421 atcctttatt ttttaaatca caaagtaagt cagcaagttt cttagtcgct ttgaagtcca
    21481 aatttgaaga cggttcatcc aatataatta atttagagtt tagcgataaa ctacatccaa
    21541 tagaaatttt ctgccgttca ccacttgata gcgaaaaaat gtttttatct aatatatttg
    21601 aaagatctaa taattcagct aattcattga ttcttttatc catatgctct tttgtaaatc
    21661 caaagttttc catcgaaaat actaactcac tcgttgtatt tgttgtaaag aattgtcctc
    21721 taggatcttg acttacgtag ccaatatttt ttgatatact ttcttgtgtt aaggattgta
    21781 gcgagttatt ttcaaataaa atttccccaa ccaattttcc gtcataaagt cttggaataa
    21841 gtccattcat taactttaat agtgtggatt tcccactccc agaagaacca gtaatcaatg
    21901 taagactgcc tttttttatt gaaaaattaa ggtcaacaag cgcttgttcc ttaacatcct
    21961 tatatgaaaa agaaacattg ttaaattcaa acaattcttt cacctcaaat caaaatactt
    22021 aagaaaaaaa tagtaacgga aaacaggatg atatagtcta ataagctcat gtttgtttta
    22081 tagtaagaac ttcttttcac aggagattct atcccctttg ttattgcatt agcggtcatt
    22141 tcttcagcaa tcatagttgc acgtataaga acgggaacta acagaaattc caaatattgc
    22201 agaggctgaa aaaaagacaa gcgaatgttt ctcattttta aagattcttt aatcattctt
    22261 atttcagaac gataagtcgg aaagaatctg atcactactg taatacttaa cattattttt
    22321 ttcggtaaat gtattttttc taaagaggat aataattcat tgctagtata gacactaacc
    22381 aacaaccatg agatcattac gaccggcaac attcgaaata caatatataa ggatgtcgca
    22441 aaaaatatag taaaaaactc tatcttgata gcatcaataa ttttcattac taaaaacaga
    22501 actgaataaa tcaagatgaa ataaatacat gtctttattt tacgaaacat caatactaaa
    22561 actgtagcaa taatgaaaca ccccacttga aatatatttc cctttcctaa aaaaataatg
    22621 accggaaaaa aaatatttaa agtaatcaag gttaacggat tgatttttga cactctttca
    22681 cctccaaaaa ataagatcgc actgttatct taagctgata aaaatgcacc gttattcaga
    22741 taacagtgcg atcttatcaa aaagcataac tgagaacaat tctcaatgca agcttttttt
    22801 aattaactca gtaattcaat ccacccagga acagaaaatt gatacaatct tctggttaat
    22861 agcatagtct tttcttttga atagctatgc ttcaatatat catagaatgt agcgtagaat
    22921 gagtaaatgt agaagtggat ttcttcttca ttaaagacta aattttgatt gatcattccg
    22981 atagtcttta acttatctag acaacagact gtctttttcg tcacaaatga tgtaattttt
    23041 tcaacaatat cttgatattt ggttccagct gattggaata aaatcaaatc gaatatacgt
    23101 ttattctcaa aaatataatt aataaatgga tctaattcat tgaagttctc tctccaactt
    23161 ttctctatat ctatattttc taagtttaag aggtatttgg taaactcttc attatacata
    23221 ttaataagcg cattaatatg cggctgaata atttcttcaa ataacgcttc cttagaagta
    23281 aaatgtttat aaaaagcacc cgtagttacg cctgcggtca ggcacagttg ccttatctta
    23341 gtttgctgaa atccttcttt ttcaaaatgg cttagtccac tttctattaa cttttggtga
    23401 gttataatat aatctctggt ttctatgggt acttcctcct aataagatga attaacttta
    23461 ttatcttaca caggacttga agtttcaatc gttaacgttt attactgaac tcattcctta
    23521 gagctaaact aatgagaaag agcagaataa ctgatacaaa aagcaatcct acaagaatta
    23581 atagactcat ctaaatcatt caccttttct aaaataatcc gagaaagatt taaaaaaatt
    23641 atatccagct attaggataa gtcctatcat aatcactcca atagccattt tcttatcctc
    23701 cctaaaaata aagtctttta cttaaaacct tttttgtaat cgtcaataaa ttttttccct
    23761 ttatcagtta attgaaatcc tatatctaaa tagtcctcat atctaagttt agccagcatc
    23821 tcacttacag atgctggact aacacttaga acttttgcta aatcattatt ttttaatttc
    23881 ttttttcttt ttgataattc ctgaattgct agtaaataaa ttagtatagt agtcgtttta
    23941 atttgtacca tacctaccct ccaaattatt tcgaaaggcc ttcagcaatt ttatccaggt
    24001 tccatttcat catcgcatag taactatcac catcttgtcc tttttctgca attgaatcag
    24061 taaaaatcgt tgaatagata ggaatattgg tatcttttga tactgttttc attggtctat
    24121 catccacact gctttctacg aataaggagg gaacttttgt tgtgcgtaat ttttcaacta
    24181 agtgttttat ttgatctggt gttccttctt cttcagtgtt gatttcccaa atgtaagcag
    24241 aaggcacatt atacgctttc gagaaatatt taaagcatcc ttcacttgta acaatcattt
    24301 ttttatcatt cggaattgaa gcaaatttag atttagcttc tttgtctaga gaatccaatt
    24361 tttcaatata cttatctaga ttttctttat agaatttttt attatcagga tctttttccg
    24421 ctaactgttt ttcaatattt ttagcgtaaa taataccgtt ttctaaattt aaccaagcat
    24481 gaggatcttc cttccctttc tcactctgac cctctaagta aataacatct atgccatcac
    24541 ttgctgcaaa atagtctttg ttttcctctt tgttcgcatt tttaactaat tttgtaaacc
    24601 aagcatttcc tccagtttcc aagttaacac cgttataaaa aatcaaatct gcttttgaag
    24661 ttttttgaac atcttcaggc aaaggttcat attcgtgggg atctttccca ataggtacga
    24721 tactgtgtaa atcaatttta tcttttgcta tattttcggt aatatctgct aaaatcgaat
    24781 tagtaactac tacttttaat ttttcgttcg aagccacttt atctttgtct ttactattta
    24841 ttgaacatgc cccgaatata aaaagagcaa tcaaaatccc cagacttccc agtactgctt
    24901 tccattttct catcataacg atctctcctt ctttcttaaa aatttttgtt ttggtgagat
    24961 tagaaaacta attacaaata agaatgccgc tgttaaaaca atacttgatc ctgctgctat
    25021 gttaaaacta taaccaataa acaatccaag caatgatgaa cttgctccga ctgttgcaga
    25081 aataaatatc atagttttca agctatttgc atataaatat gctgtagctg ctggtgtaat
    25141 taacattgca acaattaata tagttcccac actttgcata gctgttactg ctactaaagt
    25201 taatagaacc atcaatagat aatgataaaa actgacattc atccctattg cttttgccat
    25261 taatggatca aaagaagtga ttaataattc tttaaaaaat agaactacta taaccaacac
    25321 taaaaccgaa acgccaattg taatccactt atcaatatct tgtacagcta aaatatttcc
    25381 aaataaaata tgaaataaat ccgttgagct atttgcaact cctataagga taactcctag
    25441 tgctaaaaaa gaactgaaag taataccaat tgctgtatct ccttttatta cactattgct
    25501 ttttataaaa gtaataatga tagaagctaa tagaccaaat acaatcgcac caataaaaaa
    25561 attaatacct aaaatataag aaattgctac tcctggcaaa acagcatgag aaatagcatc
    25621 acccataaga gacattcctc ttaaaataat aaaacaacct actgttccag aaacaatccc
    25681 tataactaca gatgtaatta gagcattctg taaaaagtga aagtcataca gaccattaat
    25741 aaaattttga agcatattat tccccctctc caataaatat agtatctcca taggcttttt
    25801 ttaaattttc ttcattaaat acagattcta cacttccgtg agcaattaga tttcgattca
    25861 aaagaataat attatcaaaa tacttcttta ccttgcttaa atcatgatgc acaattagca
    25921 ctgtttttcc ctgatgtttc aattttttta acgtatccat aattattcgt tcactaacta
    25981 agtctatccc tacaaatggt tcatctaaaa atatgaaatc cgcattttga gcaagacatc
    26041 ttgctaaaag tacacgttga aattgtcctc cagataattc tcctatttga tgattagaat
    26101 attttcccat atcgaccttt tccaaagcct ttgaaactct ttgccattct gcatttttaa
    26161 ttctttgaaa aactttcatt ccggcatatg ttcccataga tacacattct tttactttta
    26221 ttggaaaagt aaaatcaata tcagcttttt gctctacata tgcaatcttc accagttttt
    26281 tattgatatc ttcattatta atggagacct ctccctgatg agggataata tttaacattg
    26341 ctttaagcaa tgtggatttt ccagcaccat ttggaccaat aatacctgtt aaagcacctt
    26401 cttgtatttt taaagaaatc tcgttcaatg ctaaaaaatc gttataacaa acattaagct
    26461 ttttcacttc taacataact ttctccttct aaatgataat gattatcacg atcatatggt
    26521 atacaaaaat tatattttag tcaaccctaa attttatagt ttagattttt ttctgttgct
    26581 gaagattaga atgacaaaac tatttgaaac tacgcgtata gtaagtagct ttcaaaatat
    26641 ataaaaaagg agcgttctca aatgagaaca tgctcctaaa actactttat tggcgtatgg
    26701 aaaaattata cttgaagtgg atagcgggtt gaataagttg ataagcatca aaacatttat
    26761 aaaaattatt caagactatg tctgttccat atacaaatat gaaaatcagt aaactccatt
    26821 taactttttt cgtagtgtgt ctttttatac atgtttatcg ttattaatag tgaataggaa
    26881 aaaaactatt tttgatcatt gacaattaaa tacaattttt gaataacgat ccctgctatt
    26941 cgagctagaa actcaagtac gccatgacct gattagcgac aaatacttag agatttcttt
    27001 ttgttctggg aaaacaaaat gccatgacaa tagcagcaaa taatgatact tccgtaaggc
    27061 tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta cccaaagcaa ttcaaaaatt
    27121 tcataactac tattttagga gatgatttta tagaatccag agtctacctg aataaaaaac
    27181 aaacctgtgc atttctagga atcgataata atactctaga taagtggatt cgtatatata
    27241 atttcccagt aataagagtt ggtggtgtag ttcgctttga cgtagaagat attaaagact
    27301 ttatgaatac atttaaaata acttgaaatt ttcttctcgc cgcatatact aaaattagtg
    27361 aaatatactg ccttactttt tttaaggagt gaggattttg cctacaaagc tttcaaatgg
    27421 gaaatataaa actaatctga gatatccaaa aaggtttaaa gaaataacag gaattgcatc
    27481 agaaaagtat caaaaaacat ttccaaatag gcaattggcg attaaagccg aaaatgatat
    27541 gaagaagaaa atagaaaaag ttttgagaga ggagaatgca aactcattag aattaaaagg
    27601 aaaaataacg tttaaaaaat tttacgaatc taaatggctt cctagatatg aattaggtca
    27661 aacaattaga tcaaacaggc ctcctagtga tataaccatt agtaatacaa aagatatatt
    27721 tcggttgcat attttaccta tgtttggtga atacgcaatg aattatctaa atatcaatac
    27781 tgagataata tcagatgaat taaccaaaaa atcgaaagaa tatgcaaata taaaaatcat
    27841 aaaaggatat gttcgttcca tgttcgatat tgcagaaata ctaaattata tcgaatttaa
    27901 tcgaactact aaaattattc aaagtatcac tgcacctaaa aaaaatgctt tagaagaaaa
    27961 aagaatacaa gaaggtaagc aagcactatc atcaaaagaa cttactaatt ggatagaagc
    28021 agtaaatgat gattttaata atcatttact tacttttcat gattacacac tttttatgat
    28081 taccctgtat cttggtgata gaaaaagtga gacttacgct ttgcaatgga aatatattga
    28141 ttttgaaaaa caaacggtcc gtcttaaaca tactttagat aaatatcaaa gaaaaaagtt
    28201 tacaaaaggt cgaaaagata ccgtcattca agtaccagaa gttgtaatga cattgctaag
    28261 tgaatggaaa tcggtacaag ctgatcaatt gttgaaatta aaaattaagc aaacgttaga
    28321 tcagtatctc tttacttata ctaaaccatc tggagaagta aattgtcctg ttcatgccga
    28381 ttacctaaac tatcgaatca attcaattaa aagaaggcat cctgacttag ttcatttatc
    28441 tccacacaaa ctccgtcata cttatgcaac tatagctaga caaggtggag cagatatgaa
    28501 tcaaatttca aatgctttaa cacactcaga tatttctaca acaaagatat atgttaacac
    28561 accagatata gttgataagg ctgtttttga agcttttcag agaggattga ataagtgtga
    28621 ttgatatgtg ttcttaaatg tgttcttaaa aacaaaagaa gcactttcca aataattgaa
    28681 aagtgccaga aacgttgata tataaaggat attaacgttt tgagaattgt gaagctttac
    28741 gtgctttctt aagacctggt tttttacgtt cgaccatacg tgcgtcacga gtaagtaagc
    28801 cagcacgttt aagagctaaa cggaagtcag gatcaacttc tagcaatgca cgagcgatac
    28861 catgacggat agctcctgat tgtccagcgt atcctccgcc gtttacgtta acaaatacgt
    28921 catatgcgcc ttttgtttca gtaacagcga aaggttgatt gattacttca cgcaagtcag
    28981 catgtggaat gtattcttca acatcttttt tatttacagt aattttgcca gttcctggta
    29041 ctagacgtac gcgagcaact gcgtttttac gacggcctgt gccgatatat tgaacttgtg
    29101 ccaatgaaat tccctcctat tagattaagt ttgtgatgtc taaaacttct ggttgttgtg
    29161 ctgcatgtgg atgttctgct ccaccgtata cgttcaattt tgtaaattgt ttgcggccta
    29221 aagtgttttt aggaagcata ccttttacag aagtttcgat caaacgacga gaatttttag
    29281 cacgcaattc gccagcagtg attgatttca atcctcctgg gtagttgctg tgacggtaat
    29341 aaattttgtc agtcgctttt ttacctgtta atttcacttt gtctgcattg atgacgatga
    29401 cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt atttttacca cgtagtacag
    29461 atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt tgcgtctact acataccatt
    29521 tacgttctac ttcgcctggt ttggccatat atgttgtacg cacgttattt tcctccaatt
    29581 taagttctga tgtttgacat acacttcgag tttccggggc tctttgtggg gcaaacaata
    29641 ccatttaaca tattacccgc taatctagtc aatgtcaacg tttttctgct tgattgaaaa
    29701 atttatatga aactttactt cttttctttc ttttatataa aagggtcgtt tcctgtcaaa
    29761 atgaaaaaca aaaagaggct gtgacaaaag tcttgtcaca accccttatt ttgaataaac
    29821 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccataaaaca taagaagctg
    29881 ttttttaaaa ttgtttcttg tgactcggag ctgaacggca ggct
//
LOCUS       DACEDO010000033        29255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEDO010000033 DACEDO010000000
VERSION     DACEDO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.033"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1530)
                     /locus_tag="IWO52_001488"
     CDS             complement(<1..1530)
                     /locus_tag="IWO52_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355763.1"
                     /translation="MKIIKKKIAGRKINWLALSLLALMFIVIFTGNVAHASIFNWFDD
                     TESQTEFLNNPVYQPYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIIGYKMVTGKGTVDLKSVGINVLMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISIGLIALAIAYVFSAFVIITAILELAFKRV
                     LGVLVFATDLETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRA
                     KGSGNGNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMAS
                     ER"
     gene            complement(1517..1735)
                     /locus_tag="IWO52_001489"
     CDS             complement(1517..1735)
                     /locus_tag="IWO52_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355764.1"
                     /translation="MIENRLIDQICCLDQNVDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQEMAQKLSESLKKWAEGGNNENN"
     gene            complement(1728..2693)
                     /locus_tag="IWO52_001490"
     CDS             complement(1728..2693)
                     /locus_tag="IWO52_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ0355765.1"
                     /translation="MKTLFDYILTLTGGSVEFMAEKRMLSKTFLNNASFMKMSDSAKL
                     LYVYLNVNADDDGIVEAYPYVRMLGTPDDDVKILLAKEFLYMLNEDLLVFLPHWLQNQ
                     TLRTTRYTPSIYRFKLLNSNPDFFTRLKQISIEKILKAGQTEMLRNDLIEKWYPVGLP
                     KDDHGHAQVRLGQVSSDQSRSGKVSLGEIREDQSINLSDDMEVEIMRQKIKNQIGYNA
                     LKSNPCYKRIVEDIVNIIQSIYSEKSKTYRINREEKSANEVIEKVEQLNQFDIESAID
                     SFNAIDHEIHNLNLYWITTLYNCKNTVSSRAYNNFVTQTREVLYD"
     gene            complement(2883..3167)
                     /locus_tag="IWO52_001491"
     CDS             complement(2883..3167)
                     /locus_tag="IWO52_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0355766.1"
                     /translation="MDVSSVQVILPPDLDQSLRKYFYQVAKEAVDQVRIETSLSKEIF
                     RLGEACEYLSCSRSTLKKFINQRGLKVSIIGDCQFIRKEELARFIKEYEI"
     gene            complement(3180..3473)
                     /locus_tag="IWO52_001492"
     CDS             complement(3180..3473)
                     /locus_tag="IWO52_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0355767.1"
                     /translation="MTDNEINAILSLVKTKKDEVVRLDSPVRKYRRFCGYRQKELAEL
                     LEISELTYRNKEKGRVPFKDYEIERIVKLFHKDGIHLDIVDIFFSDLKTKKDE"
     gene            3542..4306
                     /locus_tag="IWO52_001493"
     CDS             3542..4306
                     /locus_tag="IWO52_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0355768.1"
                     /translation="MNLNKTSVGNRIRLVRKELGLSMKEFGKKITPTAADSIVSRWER
                     GISLPNNDRLTQIAKIAHKDINWLLWGTFKEFISSLLDKEGYTNFLNDFPETVDTIYN
                     DLMPISENLQFPDYENSILRLFNKIYLPIFNDYCLDIIELDLEDFINKRHSISSDKFK
                     NRFLSSFHEGVSNSGYKYGDTDLIYKYGIALLEKFDDSYKLNNPYESIDAYFSAMTVN
                     TEDTEKLIYTICKSLGIPFQKGSNTAQILSKNHNNF"
     gene            4357..5502
                     /locus_tag="IWO52_001494"
     CDS             4357..5502
                     /locus_tag="IWO52_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0355769.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKKETNETFKEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTASRIFKYAMTLGIITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLESLFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(5587..5892)
                     /locus_tag="IWO52_001495"
     CDS             complement(5587..5892)
                     /locus_tag="IWO52_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0355770.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(5918..6349)
                     /gene="ruvX"
                     /locus_tag="IWO52_001496"
     CDS             complement(5918..6349)
                     /gene="ruvX"
                     /locus_tag="IWO52_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0355771.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6346..6612)
                     /locus_tag="IWO52_001497"
     CDS             complement(6346..6612)
                     /locus_tag="IWO52_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0355772.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6866..7591)
                     /locus_tag="IWO52_001498"
     CDS             complement(6866..7591)
                     /locus_tag="IWO52_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0355773.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7957..8027)
                     /locus_tag="IWO52_001499"
     tRNA            complement(7957..8027)
                     /locus_tag="IWO52_001499"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7993..7995),aa:Gly,seq:ccc)
     gene            8144..8896
                     /locus_tag="IWO52_001500"
     CDS             8144..8896
                     /locus_tag="IWO52_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0355774.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8912..9391
                     /locus_tag="IWO52_001501"
     CDS             8912..9391
                     /locus_tag="IWO52_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0355775.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(9642..11222)
                     /locus_tag="IWO52_001502"
     CDS             complement(9642..11222)
                     /locus_tag="IWO52_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0355776.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11215..11958)
                     /locus_tag="IWO52_001503"
     CDS             complement(11215..11958)
                     /locus_tag="IWO52_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355777.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12134..12910)
                     /locus_tag="IWO52_001504"
     CDS             complement(12134..12910)
                     /locus_tag="IWO52_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0355778.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(13158..13994)
                     /locus_tag="IWO52_001505"
     CDS             complement(13158..13994)
                     /locus_tag="IWO52_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0355779.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(14005..14934)
                     /locus_tag="IWO52_001506"
     CDS             complement(14005..14934)
                     /locus_tag="IWO52_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0355780.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15126..15479
                     /locus_tag="IWO52_001507"
     CDS             15126..15479
                     /locus_tag="IWO52_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0355781.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(15553..16209)
                     /locus_tag="IWO52_001508"
     CDS             complement(15553..16209)
                     /locus_tag="IWO52_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0355782.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            16435..18369
                     /locus_tag="IWO52_001509"
     CDS             16435..18369
                     /locus_tag="IWO52_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0355783.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(18455..19678)
                     /locus_tag="IWO52_001510"
     CDS             complement(18455..19678)
                     /locus_tag="IWO52_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0355784.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19672..21570)
                     /locus_tag="IWO52_001511"
     CDS             complement(19672..21570)
                     /locus_tag="IWO52_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0355785.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(21564..22682)
                     /locus_tag="IWO52_001512"
     CDS             complement(21564..22682)
                     /locus_tag="IWO52_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0355786.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22765..23598)
                     /locus_tag="IWO52_001513"
     CDS             complement(22765..23598)
                     /locus_tag="IWO52_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0355787.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(23591..24508)
                     /locus_tag="IWO52_001514"
     CDS             complement(23591..24508)
                     /locus_tag="IWO52_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0355788.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24735..25859)
                     /locus_tag="IWO52_001515"
     CDS             complement(24735..25859)
                     /locus_tag="IWO52_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0355789.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            26042..26950
                     /locus_tag="IWO52_001516"
     CDS             26042..26950
                     /locus_tag="IWO52_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0355790.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            27091..28704
                     /locus_tag="IWO52_001517"
     CDS             27091..28704
                     /locus_tag="IWO52_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0355791.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9234 a         5718 c         4750 g         9553 t
ORIGIN      
        1 tctttcacta gccattgtag ctccatctga agctaaacct gataatccac gttcatgggc
       61 atatcccaaa gctcttccag ttttgccagc tttttttgat agagtttcgc cgaaattctt
      121 ttctggtttt cgatcttctc cttctccgct gtctgatcca tttccatttc cgcttccttt
      181 agcacgatta aatccatttg atccttttct aaacagcatt gcgcccatac caaaagcaga
      241 agcaagttgc ccataacctt ctttgagtcc aatatcaaca ccaaagtatc tcatgactgt
      301 tccactgcct ttgattaaaa caaaaacggc acagatataa gcaattgttt ttataaaaat
      361 atttgttgag ataccttgtc ctgagttcag aaaattgata aacatagcaa acatggatag
      421 tccaaatcct tgaaagccta cagtcaaata gcactgtaga atatctgata gaacaacttt
      481 tgaacgctgt cctgtttcta agtcagtagc aaatactaaa actccgagta cacgtttaaa
      541 tgctaactcg agtattgcag taataatcac aaaggcgcta aaaacatagg ctattgctaa
      601 agctatcagc ccaattgaaa tgccccatag attcgcttga tagcgataat atcctgattt
      661 gataccatca acaaaggttg acaaaaaatt atctgaaaat ttagtagcaa caaattcatt
      721 atcagaattt tcaactaatt cataacgcag gttatcagct gttgagttct ctttttccat
      781 tttatcaatt acattatctg tcaatacttg cgtaagatcc gttttccaaa aattttcttt
      841 tgttagattg ttccgatcat tcgttttatc gatcatggaa tattgatccg ttttattgat
      901 ataagctaaa tcagtaaccc cttgttcaat cagcgaccat gccacaccgc catcttgatt
      961 gttaatacta gttgcgtcat tataaagagt ctttgcaaac attattcctg aaagtttttc
     1021 tgttgatcca atcaaagtcg gcattaaaag aattaatact atcgacatta aaacattgat
     1081 tccaacagat tttagatcaa ccgttccttt tccagtcacc attttataac caataatcac
     1141 tagtgaaagt atcattaagc caacaacaat agtgcttaaa actgactgat agactttgtt
     1201 caatccagca ctttccacaa agctaaacaa atctaacact tcaggaatca tattttgaat
     1261 accatctaca attgcaaaca tcgattttat aacagaccac cctatccaca atccaaattg
     1321 actgtaaaaa tttccgttat atttttttaa atacggttga tagacaggat tattcaaaaa
     1381 ttcagtttga ctttccgtat catcgaacca attaaaaatg cttgcatgag caacattacc
     1441 ggtgaaaata acgataaaca ttaaagcaag taagctcaga gctaaccagt ttattttcct
     1501 accagcaatc ttttttttaa ttattttcat tgttacctcc ctcagcccat ttttttaggc
     1561 tctcagaaag cttctgagcc atttcttgat taattatgat atgagttaca caattgtcat
     1621 tttcagctct taaatcgaga accacggtgt taaagcagat actctcgatt aattcgagag
     1681 tatctacatt ttgatcgaga cagcagattt ggtctattag acgattttca atcatataac
     1741 acctctcttg tttgagtaac aaagttatta tatgctctgg aacttacagt atttttgcag
     1801 ttatataaag ttgtaatcca gtaaagattg agattgtgta tctcatgatc aattgcgtta
     1861 aatgaatcaa tagcagattc aatatcaaat tgattgagtt gttctacttt ttctatcact
     1921 tcatttgcag atttttcttc tctatttatc ctgtatgttt tgctcttttc agagtatatt
     1981 gattgaatta tattaacaat atcttcaaca attcttttgt aacaaggatt ggattttagc
     2041 gcattatatc caatttgatt ctttattttt tgtctcataa tttcgacttc catgtcatcc
     2101 gatagattga tagattgatc ttccctaatc tcacctaaac taaccttacc tgacctactc
     2161 tgatctgaac taacctgacc taatctaacc tgggcatgcc catggtcgtc ctttggtaga
     2221 ccaacgggat accatttttc aatcaaatca tttcgaagca tttccgtttg acctgctttt
     2281 aaaatttttt caatactaat ttgttttaat cttgtaaaaa agtctggatt actatttaat
     2341 aatttaaatc gatatataga gggtgtatat cttgtagttc ttaacgtttg attttgcagc
     2401 caatggggaa gaaatactaa taaatcttca ttcagcatat agagaaattc tttagctaaa
     2461 agaattttca cgtcatcatc aggggttcca agcatccgta catatggata tgcttcgaca
     2521 atcccatcat cgtctgcgtt tacattcaga tatacatata ataattttgc tgaatccgac
     2581 atcttcataa aactcgcatt atttaaaaaa gtttttgaca acatacgttt ttctgccata
     2641 aattccacac ttcctcctgt caatgttaaa atgtagtcaa ataaagtttt cacagaactt
     2701 tattattgag tgttcttccg gtctgcaaac tgtaaggagc actctttttt tgtatattca
     2761 ccttttatta aatcaaatag aaaataaatc ttgatttctt ttaaactcaa ctttgcttct
     2821 tcatcatcgt tcaaacaaac acttcctttt tttcatatca aaagcacacc acgcaacttt
     2881 ttttatattt catattcttt gataaatctt gctaactctt cttttctgat aaattgacag
     2941 tctccgatga tactcacctt taaaccacgc tgattaatga atttttttag tgtacttcta
     3001 ctacaactca aatattcaca cgcttctcca agccggaaaa tttctttgct taaagatgtc
     3061 tcaatacgaa cttgatcaac agcttcctta gcaacttgat aaaaatattt acgtaatgat
     3121 tgatcaagat ctggcggtag aataacttgt acactcgata catccatgat gcccactcct
     3181 cattcgtctt ttttcgtctt caaatcacta aaaaaaatat ccacaatatc taaatgaatt
     3241 ccatctttat gaaatagctt tacgattctt tctatttcat aatctttaaa cggcaccctt
     3301 cctttctctt tgttacgata cgttagttcc gatatttcta ataactccgc aagttctttt
     3361 tgtctatatc cacagaacct gcggtatttt ctaactggag agtccaacct taccacctcg
     3421 tcttttttcg tctttaccaa cgatagaata gcatttattt cattgtccgt caatacttac
     3481 ccgtcgtttt tcgtagattc cattccgttt ttgtgttaca ttactgtcgg agggatataa
     3541 tatgaacctt aataagacat cagtaggaaa ccgtattcgt ttagttcgta aagaactcgg
     3601 gctttccatg aaagagttcg gaaaaaagat aactcctacc gccgcagata gtattgtttc
     3661 gcgttgggag cgagggatta gcttacctaa taacgacagg ctaactcaaa ttgctaaaat
     3721 tgctcataaa gatattaact ggttattatg gggaactttt aaagaattta tttcatcatt
     3781 gttagataaa gaaggataca caaatttttt gaatgatttt cctgaaacag tagatactat
     3841 ttataatgat ttaatgccta tttcggaaaa tttacagttt cctgattacg aaaattctat
     3901 tttacgacta ttcaataaga tatacttacc tatattcaat gattactgtc tagacattat
     3961 tgaactggat ttggaggact ttataaataa gagacattcc atctcctctg acaagtttaa
     4021 aaataggttt ttatcctcct ttcatgaagg agtatctaac agcggatata aatacggcga
     4081 tacggattta atttataaat atggaattgc attacttgag aaatttgacg attcttataa
     4141 attaaataat ccatatgagt caatagatgc gtatttttct gccatgactg taaacactga
     4201 agataccgaa aaacttatat ataccatttg taaatcgctt ggcattccat tccaaaaagg
     4261 aagcaatacc gcacagattc tttcaaaaaa ccacaataat ttttaattca ttttttctat
     4321 caaaagcacg ccacgcaact gataggagga aaaacaatgg caacaattaa gcaatatgaa
     4381 aagaaagacg gaaccaaagc ttggctattt cagacctacc ttggaatcag tccattaact
     4441 ggtaaagaag tccgtactac acgtagaaat tttaaaacaa aaaaagaagc tcaaatcgag
     4501 cttaatagac tacttgtaga ctttgaagag aacggactta aaaaggaaac aaacgaaact
     4561 ttcaaagaag tgtatgatct ctggtatgag agctataaaa ctactgtaaa ggaagtcact
     4621 caattaaaaa ttgagattaa gtttaataaa tggattcttc caaaatatgg gcagtttaga
     4681 attaaagaaa ttactacaaa acatgttcag aagatattga atcagtgggc taaaacaaca
     4741 gatcaatata aagtcctaca ttctactgca agccgtattt tcaaatatgc aatgacttta
     4801 gggataatta ctaaaaaccc ctgtgaactt gtaatcatgc ccaatagaaa cgttgaaaaa
     4861 aagaaagata ctgtcaaaat ttattcacgg gatcaactag aaagtctctt ccaatacttg
     4921 gaatcaaaaa gaagcaccta tagagccgac tacgataaaa cgctacttcg ctttctattt
     4981 tttagtggtt gtcgaattag cgaagcttta gctttagatt ggtcagatat tgactttgat
     5041 tcaaacaccg tcactataaa caaaacctta agccaaacaa aatatgggta caaaatttca
     5101 agtccaaaaa cgagaaatag ctatggttct ctatctttag acaataaaac cattaattgg
     5161 ctaaagaaat ggcaaatcaa tcaaaaaaga tatatgttaa cccttggtat tactgatcca
     5221 acaatgattt tttgtggaat ctacaaggaa atcattacta aacacgctat ttattctcgt
     5281 atgataacaa tctgcaagaa ttctggtatt ccatttctag gtaaccatgt aaccagacac
     5341 actcatgcta gtatgctgtt agagtcagga gtctccatga aggaagttca agaacgcctt
     5401 agacactcat caatcaaatt aacaatggat acctatgctc atctaactaa agaaaccaaa
     5461 gaaaaaaccg ttgaaaagct tgcagagcac ctaaatttct agcggaacgt attcaatctc
     5521 gtattcaatt caaaatacaa aaaagctgaa cccttatcta ccaagggttc agctaccatt
     5581 taaagcttat tcttcttctt ccgccatgaa tgtattgaat acttcttcga tcatatccca
     5641 ttctgcgtct gtttcgattt gttctaactc gccttctgtt ccatcttcgt tttcgatgta
     5701 tgcataagct tgtaattcta catcttcatc ttctgaaaca ccagctggat ataaaagaac
     5761 atagttacgt ccaaattctt cttgtccgtc aactgttagc aagatttcgt ataatgtttc
     5821 atttccttgg tcatccacca atgtgatgtg ttcgtgtcct tcgtgttcgt gggaatggtt
     5881 atgttctgtc ataagattac ctcttttctt tttattatta aaataattgc ttacctgagc
     5941 cgtctaaata attttgtaaa atcatcacag ctgctaattt atcgatgact ttttttcgtt
     6001 tcgctcttga agtatttgcc tgctctacta gcatacgttc tgcctgaaca gtggttagtc
     6061 gttcatcttg ataatcaacc ggcaaggaga atagttcttt gatcttctct ccatatgaca
     6121 tggaagcttc tgcccttggt ccgatggaat tgttcatatt cttaggtaat ccaactacaa
     6181 aacggtctac ttcgtactgc gccactaatt ccttcaaccg atcaaaacca aactcttctt
     6241 ctgcctcatt gattcgaatg atttctactc cttgagctgt ccagccaaag ggatcgctca
     6301 cagcaacgcc tactgtctta gaaccgacat ccaagcccat tactctcata aattcactcc
     6361 atgatttgag aggtagtctt tgacaattac ttccatgatt tcatcgcgtt cgtgacggcg
     6421 aatcaaattt cgtgcatcac gataacgagg aatgtaggcc gggtctccag aaagcaagta
     6481 gccgacaatc tgattgattg gattgtagcc tttttcttcc aaagcccgat aaacaatcgc
     6541 taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc
     6601 tgtaaaaccc atgattctac acctctttcc cgcaaggtaa ttccagctta ccttctacac
     6661 ccttatttta ctcgaagaaa gggaaaacta caaacaaata ttccaaactt ctgaataaat
     6721 taagaaacac gatttgtctg atttttcctt ttttctgtgg ctttttcaaa agccctcttt
     6781 ccataaagta tacgctatat ttctttgaaa gtatgttttt acacaatttt ttcataaagt
     6841 tcataaaaaa ttcgaattgc tgctactaat ttttcgtttt cgttgcttgc cctgtctcat
     6901 aattgatggt gtagccatct tgaacattgt agataaagac attgaattca atttttttgt
     6961 cttcaatgct ttctgcttcc aactgcacac ctcttgctac cagttcattt ccctcgaaat
     7021 gaggagtgac acgatagcgt acatgatgat ttgtttcctt gatatatgcc gcaatatcat
     7081 tttcgtgggc aagcatgtac ggagcattca acgaacgtgt ccctgtcatt aagtttttaa
     7141 tgttattatt ttgaccagtc aactggaaac caatcaaatg gctgcggttg tataaccatt
     7201 gtccgtcact taatttcttt tgtttccaac ctgtcggatc aatatacaaa ggttctctct
     7261 tttcagtagg aaacaagtct tttcccaaca tcgcattggc ttgtcctact ctgttcaatt
     7321 tgtctatatt tgaatagctt tcccatgtcc ctttggaaag actaagatcc tcttttgtaa
     7381 actccggctg attcccattg atttctattt cttgatgttt tccatcatat tctggcacat
     7441 ccgctacttg agaagcttgc ggtgtggaat caaaaatggt tgtaatttgt tggaagatat
     7501 ctgaatcttt ttgtgtggtg gaaataccga gtgcccctgc aatcacagca gcgacgatcc
     7561 ccacgatcgc cttggttgtt tttttgctca tttctttact cctttactaa cagttcccta
     7621 aatcttaaca tatgttcccc tataaataaa tactggaaag aaatagaaac agtaaaaaaa
     7681 taaaaccagt ttttagacgg cttaagaata caaaaaaaga gacggattca tcgtctctac
     7741 tcaatcacaa aacactattt acaaggtatt tattatcatt caaaaaattt ttcttaatac
     7801 tcaaaactaa acggcttctg tacgacctct tggtaaacag tgttcaaaag cttgcatctg
     7861 cggtaataag tccaaccaag tcaaaaacat aagaagcttt tttgcttgac tattcttatt
     7921 acttgatgca gggcagcttt ttcacagcct cttttaagcg ggtgacggga atcgaacccg
     7981 cgacatcagc ttgggaagct gggattttac cactaaacta cacccgcgat acaaaaacag
     8041 tatacgcctc tttcactcct tcctcaagtt tttttattct ttacgcttcc gatatgtttt
     8101 gtagtaaaat aaaactaatc aatcaattat tggagaagat gctatgacgg gaaaagaatt
     8161 agatctgttg ctggacactt gtttactcgc tgggaaaatc atgatggaaa gcaatgcgga
     8221 gatgtatcgg gtagaagata cgatgagtcg aattgcttta gcttctggaa atttccgttt
     8281 ggtcagctat gtcactcaaa ccggtctctt tattggtctt gatcgtacct cgaccatccg
     8341 aatggaacaa attaccaacc gttcgattaa tttagaaaaa gtagtaaaag taaatgatct
     8401 ttcacgtaaa tatgttgctt cagagctaac attagaagaa ctttactgcg aactgcaagc
     8461 aatcgaccga gaccgttctt tctttcctgt ctggttgcaa atcgttagcg cggcagtgat
     8521 cagtggaaca attatggtgt tatttggcgg aaagatgacc gatttacctg tgactttagt
     8581 cattggagga ttgggatacg cagcttatct atatagtttg aaatttttta gaatcaaatt
     8641 tttatcggaa tttttatctt ctttattgat tggcatagcc gctatctaca gcgtacgttt
     8701 tggcttagga agcaatcagg atctaatcat tatcggatgt gtgatgcctt tagttcctgg
     8761 agttcaaatt acgaacgcta tccgtgattt gctagctgga cattatgtct caggagtttc
     8821 tcgcggaaca gaagcgatga tgactgctac gatgatcggt tttgcgattg cttttatttt
     8881 ccagcttttt tattaggaaa ggaacacaag tatggggacg atatttattc agttttcttt
     8941 tagtttttta gcgactgcgg catttgccgt tattacaaat gtacctaggc gttcattgat
     9001 ttgttgtggt ctttctggtg cttttggctg gatgatctac tggatctgtg ttcaaatagg
     9061 cggatctgct gcttttggtt cattgcttgg ttctttagga gtagcattca tcagcgatct
     9121 tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat attcccggga tggttcctct
     9181 agtccctggt ggtttagctt atcaagcggt tcgaaatctc gttactgaat cctatcgaga
     9241 agcggctcat tatgcggtcc aagcaattat gattgctgga gcaatcgctt taggtcttgt
     9301 attatcagaa gtctttaacc ataacatccg taatttcaaa gtaaagcgtg acgcaatcat
     9361 gatgaaacga aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa aggttgtgaa
     9421 agagacgttc agccttgagc gatgacaaaa tatctcacaa acattgcgac aagtaaccgc
     9481 aggagcaata agaagtaatt atgagtatta aaaaaagttt tgtaaaaata gcttctcaca
     9541 tttttacata aaattgactt aatactgaga aactgacttt tgaacaccct ctatctgtaa
     9601 aaaaggggct gcgacatgat ttttgtcacg gcccctcttc tttattcaaa ttttttccaa
     9661 aacgttcgtt gataataaac gatccataga gcggaaagta ttagaactaa accgatcaca
     9721 ggaatattca ctaaccaaaa tggataaaac aaagccaaca ttccgtataa gacgataaga
     9781 ataatactga tgatcaatga acccatcata gttgcgacta aacccaaact tcctgaacct
     9841 ctgttaaata attggttaat attggtccag ttcagcagca gcaaccgata atctctagcg
     9901 aaataacgta gtgacaataa gtagcagccg aataaagaac caaatagcaa gctagggata
     9961 aaaaacaaag gcagctgaaa agacagtccg cttaataatg cgatcccacc agttaaaaaa
    10021 ctctgcaata tccatccgaa tctgaacttt tcttttaaat attggttcat agagattggc
    10081 aaagaacgga taaacagaaa attttcttga tccaaagaaa taagattgct gataaaagaa
    10141 gtctgattaa ctgttaagaa agccaatgcg atccctacca ggaaaacaac accgattaac
    10201 ttattatcta aatcagttaa ttttatttcg cctcctaggg caaaagcaag aataaaaatg
    10261 attggagtta acaaggaaga agaaagaact tgcataatca aattaggttc ttttactaat
    10321 tgagcattgt aactaaataa aagctgatgc acattttggt tctttttatg ggttcttttt
    10381 attttgaact cactgtttga atcagtcagt tgctctgtaa gctttggtac tatcagctgt
    10441 tttacagcaa ggaaaaatcc cagagccgtg atagctagta ttccccaacc caataaacct
    10501 tgttgagaaa gtggctgact ggcagcatca tagaatggca ataatatcga aatcggatgc
    10561 cgatctgtta actcattttc aatagtcccc gtttgatgat tcatccacat gattccagca
    10621 aatgcgatca gcatcgagct gccaagcaag atactagaaa ctgtttttct atgtttttta
    10681 aacactgctg ttcgagccaa accaaaaaca atcaaactag ccagacaaaa aagaataatc
    10741 aaaaatagag aaaacaaaag gattgagata atgatagtaa gcaaaatccc tagatcagct
    10801 cgccatccgg ttaaaataaa catgactaga agcggaaaaa caaaaggaat gatggtcaaa
    10861 gcaactacca aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag aggcaaaaat
    10921 acttgcaggt catttccttc aaaaaagata ttataaatga ctgatatact ttgagttaaa
    10981 cctaacatac caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt aacaaagttc
    11041 atagcaaaca tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa aagattttga
    11101 gacaaaagat agatagttaa tcgctttccc ttttttcctt tctctcttgc tttattcgtg
    11161 agctgtggat taacatataa aagattaact cgaattaatt ctaataattg tcgcttatac
    11221 atacggtcca tctcctgtcg cttgccttcc agccattttc aaataaatct tttctagcgt
    11281 ttcatcaggt gagccattca ataattcact aactgccccc tgataaatca attcaccttt
    11341 cttcaaaata gccagtttat cgcataactg ttgtgcggta tccaatacat gtgtagaaaa
    11401 aatgactgtt tttccttttg cggcatgagc cttcatcatt tctttcaaat caaatgctgc
    11461 ttgaggatca agtccttgca aaggttcatc caatacccaa atatctggat ctggcagcaa
    11521 cgtgccgatc aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga ttgtttcatt
    11581 ttgatgacta tacatatcaa aaagcgctgt caattctgct aaacgctttt cttttttttg
    11641 tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat tctccagccg tcaattgtaa
    11701 aaaaatatct ggggaatcag gcacataacc gattttttgt ttgatactca atcgattttc
    11761 cgtcaatttc attccatcta ctaaaatcgt tcccgatgat ggttcaataa tactaactaa
    11821 acttttaatc gtagttgatt ttccggcacc attatgtcct aaaaatccaa aaatttcccc
    11881 ttgtcttatc gagagagaca agtcttttaa tgcttgtttt ttcccgtaac ttttacttac
    11941 attgatgaat tcaatcattt ctgtactcct tttgtttgtt attacttttt attgtaccct
    12001 tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata gaaacgacta
    12061 ggctgctact tgggttgttc tcttctacag gaaaacactc ttctaacagg taagatatga
    12121 gtctttgttt tctttaaaat gattcatttt ctatcttttc ttgcataaaa ttctttagtt
    12181 tatcaatact tctctttccc atctctagtt gttctccatt tgcaaatgtt actatttcgt
    12241 ttactctgct aatagatgta atgttttcag tattaatgat ataagctttt aaatctttac
    12301 ataaatactc atattcgagt gattttttat attttttaat tgtgccgttt acgagaatct
    12361 cttcatcttt caatactagg cctattttat ttttataacc tggcatgctc attagataaa
    12421 ggatttcatc gtaatggata tagtgatcgc tattaccata acggacagtt gtccaccttt
    12481 ttttcgtagc atgttgaaca ggatagtttt ctttttcttt caataagtaa ctttggatgc
    12541 tttttttatt tttttcataa gtgaattctt ttatcaaata accactaggc gtaatctttc
    12601 gattaataac agttattgct ttattcggat gagacgttag ataaacaatt ttacactttt
    12661 cattcttttc tctaagcatt tcgcctagtt cgataccgct ataataggcg ttcaaatcga
    12721 tgtctaggaa aaaaatatct gaggaataga tttcattatt tttaatgaat tcaacaaatt
    12781 gtataacgtt caatccctga acgatctcaa atttccaagg acttaataaa attctggatt
    12841 cttccaaaat ttctactagc attttttcaa aaaccaaact atcatccaaa ataaatatac
    12901 gcgttaccat aaaaagtacc tccactcgat attttactat caaaatcatt tcatacaact
    12961 tttcaacata catgttagga ataaattgta attctcttca caatcttttc caacaccttt
    13021 gaaaccgata tcaaatactt tcaggatgct gcttctaaaa taaatatcaa acaaatgaat
    13081 acatcatgca aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc tgagctatgt
    13141 ttatttattt tgttctttta aaaagaacga tacacatact gatttttcgg agcagagact
    13201 ttctcagcgt ttgtaacaac tacagatggg atttcacgct tgaatggttg ataatagtct
    13261 gattggatgg ttccttctat tttataccat tcatcatttt taaccgttac tccttccggc
    13321 atatgcgtca acaaaccaaa gactcctgaa tctgccacac aatggataat gccgaatcgg
    13381 aacaagaaga tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc ttcatagctg
    13441 atttttttgc caatgaattc gctagggtaa ttataaatta gctccataac ttctaaatag
    13501 ttttcatctg tgatcttgat agtttgctta tccccatact tttctttcaa ctcattcatt
    13561 tggtctaagt agtctgattt gttgaaataa atactggtat ccggctttaa gtattgtgtc
    13621 tgcatctccg gatcccctac tgattcttta cttactggga aattaaatcc tttggcttct
    13681 acgatcgtag tgtcgagact aactgttgga aacaacgtcc ccacgattac tggcaaagct
    13741 agcagtacat aagcgattcc acgttgataa ggttttgaca acccatggtc atgatcgtgc
    13801 tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca aatacagctg aactaaagcc
    13861 aaaagagcag ataaaatcat tgaaagaata gctaagtagc gataatggac attgatatat
    13921 tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt cgaaataccc agataaaatc
    13981 aaaaaccgca gcatattttc cctcctaaat aaataatgtg aatccaataa cgctaagggc
    14041 aatcagtccg atcagcgata aaataaagcg tcctgtaaaa tatcgtttca tcatcagtag
    14101 atttttgata tcgaccattg ggccaaagac caaaaaggca gctacaggtg ctgtgccgaa
    14161 cagacttaac aaagaagatc ctataaacgc atctgcttct gagcaaagag acatcgtaaa
    14221 ggccaataac agcatgatga taatggctaa aatctttgtt gagccgagcg ttagcataat
    14281 tctagttggt aaataagtct gcatcgcagc tgcgattaat ccgccaatga tcaaataacg
    14341 acctgtatca aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt gatggatcag
    14401 ctgatttttt ggttcattct tctcactttg ttcttcttca tggacatgat gatggtgatt
    14461 ttctgctact ttagacgtga ttttttcttt caatacagcg tctttttgaa aataggcaag
    14521 ccagacacca atcacaaggg caacaaaaaa gctgcctatc acccgccagc ttgccatctg
    14581 ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg attggattga tgatcggagc
    14641 agtcagcata aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa attgatgaac
    14701 gatcggtacg atgccgcatt cacatgaagg aaagaaaaat cctaagatcg aaccagtaaa
    14761 aatagagaag atacgatttt caggaagcca tctcttcaca cgttctgggg tcaaaaatac
    14821 ttgtaaggcg cctgagataa gacagcctaa taatacgaaa ggtaatgctt caataacgat
    14881 cgataagaaa atcgtactca tctgcaaaac cgaatttgga ataaaagaaa acatgagttt
    14941 cctccttttt atttctagtt cattcgcttt gcaaatatac gcttttattc aaaaaaaaca
    15001 agttgctctg taaattttta cacaactctt attttttacc aaagcacttc aattgacttt
    15061 ttctgttatt cccattaaat taaggtctca tacatatcac ttctatcata aaagggagaa
    15121 taaaaatgaa acaaaccact aacttgactg aagttcaaga catactgcaa cagtcatccg
    15181 ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt cgtccacgac
    15241 tagaggaact tctcacacat tatgatattc cggcacttca tttggatgca tttgaaacac
    15301 cagaagtagc tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt tttcatcaag
    15361 gaaaagaagt ctttcggcaa gcacggttca tcgactttaa aaaaatccgt tatctactag
    15421 atgaactgac agcagaagat gattcccttt cttatgaaga aatcttccga acaaaataat
    15481 cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt tttgccttca tttgttcaat
    15541 cgctttttat ttttattctg ctgtattttc ggaatgcgca tttgcgtcag aatgcaagaa
    15601 atagatcaag gtttgcaatt cattcgtcaa atcaacattt tggatcaata catctgacgg
    15661 agcattcaat cgagcagcgg taaagttcaa gattcctttg attcctgctt ccgttaactg
    15721 attgactact tcttgtgctc tttcagcggg tatcgttaaa attgctactt cgatttgttg
    15781 gatcttgatt tgttctgaca tatcttccat tggataaacg gggataccgt ctacgatgcg
    15841 cccaacgatt tcctcattca catcaaaagc acaactgata cgtatgctat tactttgatg
    15901 gaatttgtat tttagtaaag cacttcccaa gttccctaca ccgatcagcg caacatttgt
    15961 caattgatcg tcgtttaatg tctttgcaaa aaagtgcata agatcttcta catcataacc
    16021 atacccacgt tttcctaatt cgccaaaata agaaaaatct cgacggatcg ttgcactatc
    16081 tacttgaacg gcttcactta attcagttga agacactttt ttctttccag cattatttaa
    16141 aatccttaaa tagcgataat acaaaggcag tcttttagct gtcgcctttg gaataatatg
    16201 atctttcacg aaatgacctc cacgtattgt ctaattacat ctttgatttc tccatcatag
    16261 cagtatttat aggaaaaaat caattccatt gctcagatat tgtgaaattt tattctttat
    16321 tcatttattt atcatttctg caaatctcgc agtataatgg aatttatggt agactactaa
    16381 ttgaaaaata gttttttatt tgaggagaat tcacaaagta gaaaagagga ttccatgata
    16441 ttattacaag ccaatcagat tgctcgccat tttggtgcag aagtcttatt tgaaaacata
    16501 catttagaaa tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg cgctggaaaa
    16561 tcgactttat taaagatcat tgctggaatt gaagcacctg atgaaggaaa gatcgctaaa
    16621 aataaaacag cgactctcgg atacctcgct caggacacag gacttgattc caatgaaaca
    16681 gtttggaacg agatgctgaa agcttttgaa caagttagac aaatggaaga gcggatgcgc
    16741 gagctagaga ttttcattag cgaaggtacg ccagacacac ctgcttatag ttcttattta
    16801 aaagaatacg accggctgca acacgatttt tcagatcgaa acggctatgg ttatgaaaat
    16861 gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt cattttatga tcagaaaatc
    16921 gatactttat caggcggaca gcgcacacgt ttagctttag ctcgcatgct tttgcaacac
    16981 cctgacattt tgattctaga cgaaccaacg aaccatttag atatcgagac actttcctgg
    17041 cttgagaatt atctgcaaag ctatactggt gcattattga ttgtttccca tgaccgttat
    17101 tttttagata aagtagtcaa tgaagtctat gaattaagca gaagaaaaat gcgccattac
    17161 aaaggtaact attcgaaata tctagatttg aaagcagaac agttagcaag tgaatggaag
    17221 gcttatgaaa agcagcaaac agaaatccat aaattagaag acttcgttgc tcggaacctt
    17281 gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa agacattaga aaaaatggac
    17341 cggctggatc gtcctcaagg ggatgaaaaa caagcaaact ttctattcag tatagaaaag
    17401 ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta tcggatatga taatgtcacc
    17461 ttatctgaac caatcaatct agatatccgt cgtcaagaag cgattgctct agtaggtccg
    17521 aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc accagatccc ttttatccgt
    17581 ggaaaagaaa cgctagggac aaatgtcacc atcggctatt acgatcaagg tcaagctgat
    17641 ctgcatacaa acaagacgat cctagcagaa ttgtgggatg agcatcctac cactccagaa
    17701 aaagagatcc gaagtgcttt aggtgctttc ttattttctg gcgaagatgt agaaaaacca
    17761 atccctctgc taagtggcgg tgaaaaagca cgggttgctt tagccaagtt agcgatgaac
    17821 aaagaaaatt ttttgatttt ggatgaacca acaaaccatt tagatattga caacaaagaa
    17881 gtattagaaa atgcattgat cgattatgac ggcacactgc tttttgtttc ccatgaccga
    17941 tatttcatca atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg cagcaaagtc
    18001 tatctaggag actatgatta ttatttagag aaaaagcagg aagaagaaga aatcgccgaa
    18061 ttgttggcac aagatgagcc agaatctgct cctgctccca cgaacaaatc tgattattac
    18121 caaagcaaag aacagcaaaa gcttatccgt agccttcaac gaaaaattac tcaggttgaa
    18181 gaagaaatgg cgcggatcga tgaatggatc gatcagttgg aacaagaaat ggtcgcgcca
    18241 gaaaacctgg aagatcacgt aaaactcaat gagctgaacc aagatttaga agctgctaga
    18301 caagatcaag aaacaaaatt gacggaatgg gaagaactaa gcttggaatt agaagaatat
    18361 gaaaattaac gaaaaaacca aaacagaaac cattcatgct gttttggttt ttcttttttt
    18421 attttatcaa acaaaaattc catatttacc tttttcacca ataagaagac caattactag
    18481 tcattctcac taaagcttct aaataatagt aatcacccca aatacaaggt gtatttaccc
    18541 cattattttt gggcttatta taaacagact gtgttaaaaa gccatcttct tcttccgttg
    18601 tagcatatct ttctttcaat tgtcgcataa ttttccaaac agcagactgg tataagttct
    18661 tttctgtttt gtctaataga cctgattcta ttaattctaa taatccacaa accatgatca
    18721 ccatactaga tgtatctttt tcttctctgc tagaatcatc aaagatcaaa tcccaataac
    18781 aaatggtatc ttctggcaac cgattcataa aataattggc taatattttg cttaaatcaa
    18841 aatatttttg ttctttcaaa taacgataac ttaatgcaaa accgtaaatc ccccatgctt
    18901 ggcctctagc ccaacaggaa ttatcactat aaccttgatg cgtacttcca tatcttggtt
    18961 ttccatcttt gacatccata taaaatgtat gataagttga accatcttct cttactaaat
    19021 aatctgctgc attttgaata tgagcctgag cagctttttt atatttttga tttccagttt
    19081 cttcactcgc ccaatacaat aatggaagat tcatgcaaca atccatgatc attctacctt
    19141 gttgtgaagg atcactagga tctccccaag cttgtataac tcctgccttc tcccaaaaac
    19201 gctgaagtaa tgtttctgca gcatcaattg aagcttcctt catttttcga tttcccgtta
    19261 ttttataatc tgctacggtt gataaagaat acaaaaaacc aagatcgtga tgattagtga
    19321 taatttgaca gtcaatcctg tggataaaat ctatatctgc tttttcagct gtttgttgaa
    19381 ataaaggatc acccgtcttc tcaaataaca accaattaat tcctgtccaa aatccttctg
    19441 tccaaccaat attgtctctt aatggataac gtccttttat aggacactcg cttgggaatt
    19501 gatactgact gttactgata cgtttgatta acctctccgt gtgtctgcga caacgctcga
    19561 tctcattatt ccattctttt tccattaatt ctttataaca ttcatttatc catttttctg
    19621 gatgtattat ctcttcaaac atcactttat caagcatttc cccaccagcc tttagcataa
    19681 ttttagtctt tttttattgt ttttattttg gatagttagc ccggttgtcc catcaaaatc
    19741 aattttggga cgattgttcc atacttctaa actataatcc cgatcttgat gagcaaccac
    19801 tcctgttccg agccaatggg ttccttttcc aaatgtaccc tctaatccag gaataaaagt
    19861 gttttcccat gtcatcaagt ttgtattggg caaacccata atagctgtgg attttcgttt
    19921 aatagtatcg tcacttaaac agataatacc tgaaaagcca gcttcactca ccctatagtt
    19981 ttccttgtct tcttctatga ttattccttc tttctcagga gaacattcaa tagcaaaccc
    20041 tgtctcatat agaacatatt ctgtatctaa ttctactttg tgtacgcgaa tctgccagcc
    20101 ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa gtaggttccc atgtagaggc
    20161 aagtacctct ttcttacata caatgtctcg tacattattt cgaacgatga actgtccata
    20221 ggttttgatg ctctcattct taaaactggt cgtgatatat gtgtccgctc gctgaatccc
    20281 tagcattgaa tccattgcca tttctgcttt caattgattc gctcgcggta tcgaaaaacc
    20341 aaattgacta gaataagcaa attttaaata tttttcattg gtcaatgtat ggaaatttgg
    20401 ccctaattgt cccgcattca aaaaaaatgt atgtccatta tcatgttgta tgagcaaatt
    20461 ggcttctgaa agaagtttag ttccttcacg aataggcata ggcatttctt tagccgtcca
    20521 atactcatgg tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc ataatggaga
    20581 taacgtacta ttatagggtt cagtcaatgt caattgaggg taggtataac caatattcag
    20641 gatacctttt tcatcaaata tttctttttg catccaccat cttatattcc gattgactaa
    20701 ccccttgatt tcacctaatt taaaaggcaa aagatcggcc cacaacatag ctgaccaaaa
    20761 gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg caccatcttc
    20821 cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct cggttcttat atatctggct
    20881 aatgggcttt tctggttcta gtacgctata aatcaaacca taataatgaa atgcaaaagg
    20941 attataataa tcttggcgac ctcttgaact gtctcgatac cagccatttt gaagataaca
    21001 attttctata ttatcaaatg cttctttcag tgctgattct ttaactggat aaccaaattt
    21061 ttggataatt gttccgacaa ttatcttaaa aaattgccaa ttaccatctg cacattcata
    21121 ttcaaaaatt tgcgagaacc aatgaataat ctggcttttt tctcctgtat ctaatttttt
    21181 ccataataat tgttcatgat gatataacgc taataacatt gcgggcattt ctaccatacg
    21241 ctggtctctt tcatgtattt ctccccaata atcgggatgg gaaggatctg ttccagcttt
    21301 tattcctgat agaattaatt tcaaccatcg ttcatctaat tggtcaattg cgggccccgc
    21361 tccccataaa acccgagaga atccttccat tcctgcaatt ttatttccat atcctacccc
    21421 ttgatgtata taatcaattt gtgcgccact tgctcgataa aacggctgga gtggttgatt
    21481 tatcttttta aataagtcaa agtaatcctg tctggtcctt aatagtggta ctggatgttt
    21541 cattgtattt tctcctcttt ttactaccaa atagctaatt ccgactgttc taatcgaagt
    21601 aagccttcaa taaaaaaata atcagaatag atcagtggca cattataagt cagttctcta
    21661 tggtatgctt ctgctccgcc tccaatgatc ccatcttcat ccatattcca atttgcatag
    21721 tgcttttctg tcttttggag tatattgata gcagcttcat gatacatttt tgatcgtaca
    21781 gaggaaacat atcctgctaa ttccagaaga ccgcatgcag cgcatactcc agcactggta
    21841 tcaatattcg aatcttttac agcaaaatca atgagaggaa tataaccagt tttttcgatt
    21901 ttctgaataa aatttactgc tatcttttct gctatagcta gatagtacgg attttttgtg
    21961 taacgataac ttaacgcgaa tccatataaa gcccatgcat ttcctctgct ccaacaagaa
    22021 ttctcattgt atccttgccc gccaagtaac cgaatatatt tccctgattc tgcatcaaat
    22081 tctgcgatat gcccggttga tccatctttc ctcactagat acttagctgt tgtatctgca
    22141 tgctgccgag caattttaga gaacctcgga tcaccagttt ccctcgaagc ccaatacagt
    22201 aatggtaaat tcatcatact atcaataatc atccaaccag gtttatggtc attccatgct
    22261 actaaatagt tccctgaaat attatagcgt ccagcaagta ccgtagctgc atgtaaccct
    22321 agttgtcgac tatgtttgtt ttgagactgt tgataatgtg caatagccga tggtaagaat
    22381 aaaaaaccta catcatgatg aacgtctgaa aaatggtaca gggcagattc taataattgt
    22441 tcttgttgta ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa gagctgccat
    22501 aaaataccac tccaaaaacc attggtccac cagtcgattc ctctgtcctt tattacgtct
    22561 gtatattttc cgttgtaaga aatatatgga attttattcc ccattctgtc taattccgca
    22621 tctatctttt cagaagctac attcaaaatt tttcttgacc attccttcgt ctgttcattc
    22681 atggatatcc cgccattctt ttaattttta tgaatgtgga gttgctccta gaacgaacta
    22741 gatggttaac attcgttatt ttttttactt atttgcagct ccaatgtcat tctccttaaa
    22801 tttagactgc gcccacacat aaatcaagac cattgggatc actgaagttg ctaaaattag
    22861 cataattgcg ccataattaa ttggctcacc tgtactagaa accgtcatat ttttgataat
    22921 taagggaacc gtatatttat ctttatctga aagcatgact aatggaaata gatattgatt
    22981 ccaacttccc ataaagataa gtaaaccact agtaataatc gtaggtgata aaatcggtac
    23041 agcgattttc gtaaaaatca cccactcatt ggcaccatcg attctggcag cctccatcag
    23101 tgctgtagga aattgactgc tatattgtcg catcataaac actgaagttg tactggcaat
    23161 cgaagggatg atcacacctg cataagtatt cgataatcct aaacgactca taatgtaaaa
    23221 taaaggaatc aatgtggtaa aaaaaggcac catcctagat agcataactg tattgaacaa
    23281 cagtttattc cccataaaac gatactttgc taatgcataa cccgccattg tagaactggc
    23341 tgtagctgaa actgtactga ttacagcaat aacaatcgaa ttccaaataa tcaagggata
    23401 attatatctc gccatgatgg cttgccagtt ttttaatagc atatccccaa acttgaaatt
    23461 gattccgcca cttaaaattt cttgattact ttgcgttgaa gctacaatca tatataaata
    23521 tggcatcaaa aatattaagg caccaaacac aataatgacg tacgtaaaaa ttctgctttg
    23581 tatcttttgt ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc gataatataa
    23641 cagatacaaa caccatcgtc caggcaatag cagaggcttt tccaaaatca accatttcaa
    23701 aacttgtttt atataaattg actcctacga ccaaagctga attttctggt ccattggaag
    23761 tcccaaagaa tatatttggg atattgaaat tacccaagcc attgatagtg gctaaaactg
    23821 tggccattaa aatgattggc tttagcatgg ggatcgttaa taaaaagaat tgccgaaatt
    23881 tcgatgctcc atctatttca gcactttcgt atatctcttt agaaatccct tgaagaccag
    23941 ctaaaaataa aatagtgtta tatcctacac taagccaaat catcgtgatg atcattgata
    24001 ttcgtatcca aaaaggattt gttaaccact gtacaccgtt aattcctagc gcattcaaaa
    24061 atccattgaa taacccagtc ggattgaaaa aagtcatgaa tacggaagct acagccaccg
    24121 ttgatgtaat ggttggaata taatagacag ttctaaaaaa tccttttcct tttaactgtg
    24181 ctgaattcaa taaaacagca aagtaaatag ctaatagcat cgttattgga attgatcctg
    24241 ccatgataat caaaacattc gtgtaagatt taataaacaa tggatctcgg aagatagcta
    24301 gataattgtc aaatccaaca aattcaaatg agtagccaat tttcataaaa ctaaaagcaa
    24361 acgagataat gatgggaatc aaaatcgtca caataaagaa aataacaaaa ggtgtgacca
    24421 atccataagc acttttttta tgagacgtat ctgttgcttt tttcttgctg ttctttattg
    24481 tcttgcttgg taacgaagat tgcatcatcg ttaagtcctc ctaatatttt ttaatcacca
    24541 gttaatttta cgccgtactt atgagcaaaa ttttctgctt cttttagata tgaattttca
    24601 aaatcacctg tttgccaata gttatatgca gcggtagtca aataagacgt agcatctgaa
    24661 taagtggctg gtaaaacagt atttgatgta ttttcattga ttttattcaa tagttcatag
    24721 tatttttgtc ctccttagta gtcaaacttt ttctcagctg cttctgtttg ataggccaat
    24781 gagttagatg tagccacgcc cagctccagt gcgttggcta agcattcttc atctgtcagc
    24841 ataaatgtaa gaaattgttg tgccacttgt ggatttttgg catctttagg gacataaaaa
    24901 cttgatcctc ctgatactgc accttttcct ggattttctt ttgaaaaagg aatcagtgga
    24961 gccatgcgcc atttgtcaga tgcatcagga aaattcaaag acatattcgt agcataccag
    25021 ccgccagcaa caaacattgc agattcttga aatgctttct cagcatcttg tgcaccgtag
    25081 aaatgtacaa tatctgagtc gatcatttct ttgattaact ttgcagcatg tttcgcttct
    25141 ttagtattca gacggatatt tccttcttca tcaagcaaag attgattctg ctgggctagc
    25201 atgttgataa acaattcttg gtccccagat tctggcaagg caattccaaa actaccagtt
    25261 ttttctttta atgcttttgc cacctggata tattcttccc aagaactgat ttcttcaaaa
    25321 tctacaccta tttcagcaaa tttttttggt tgataataac aaagtgctgg ggtaaaatca
    25381 ctagcccaag gaataatcgt ttcattactc gtttgttgac gcaaaatatt caatttttga
    25441 tcataaaact gatcgccata tttgtcataa aatccatatt catcagcact ataaaaagca
    25501 tcttgaaatt tttccagcac acctgctgct gaagtgtctg ccatggagat aatatccggc
    25561 atatcttcgt gtgttaccaa ttttggggtg atcatttgta ctgttgctgt tcccgaagct
    25621 acattcacaa tttttaagtc aatatttgct ccagttttgc ggttatagat ttttgcagca
    25681 ttttcaacat agtttgtcag tttgccgttt gcgacatata cttctaatgt agtggctttt
    25741 tttatcattg gatctgcgat gtttgctgtt acttcttcgt tagtttttcc acatgcactt
    25801 aagctgaata atagggtaat gtttagcaaa gctatcacca atttgtatct ttttttcatg
    25861 tctatccctt ctttcttttc aagttcttat ataaaacgct ttcacttatg tatattaacg
    25921 gaattagtaa cacaaatatt tatataaaaa aatattttac ttgtcgtttt ggcttttcat
    25981 ttttgttata atagtcatta attataaata gaaagctttt tatataaaaa gagaaaggaa
    26041 agtggttctt ttgttaacaa acaaccaatc ctccaatctt gtcaaaaaaa cactaaccta
    26101 ttccatagat acccatcttc atttatctgc tgctccctat tttcgactac taataaatga
    26161 aagcgctaat ttatatgctg aatttgcaca taaatctata gaactagata atccttccct
    26221 tttattcatt gataaacagc aagaagtcca aatcttttct actcaaaagg aaacacaaac
    26281 ttttatcatt atccagtttt attttgatga gcaattatta gcacatatta cgggacaatc
    26341 tttttctata gatcaagaaa taacatctat cattactttt ctgaaaaatg aacagttcaa
    26401 aattgattat ctaaaacata gtaagccagc tatttcctat gatcaggaaa tcaattcttt
    26461 tttcatacta tgccaacaga tgagcattca tttgctttcc ttattagtta cttattgtct
    26521 gcaaaaaatt caattgtctt ccttaccaag gcattatcaa caggaacttg ctttattgac
    26581 aataccttca aatgagaaag tcattcctaa aaaaaagcat attctttatt cagatgaatt
    26641 agttgatcca atcatacgat atttagaaga acatctttca gaaaattttt tgcttgaaca
    26701 attagcccaa atttttttta tcagtccagc cagcttaaaa aaacatttta gaaaagtgaa
    26761 tggaaaaagt gttattagct attttaaaga actaaaaatg gaaaaagcaa aacgtctcgt
    26821 gctagaaaat gaattaacct atacagaaat tgcagatata ttaggatttc attcgatcca
    26881 tcatttttct tcagctttta aaaaatatac agggttatcc ccttcaaagt attatctatc
    26941 cttaaaatag aaaaaaattt ttaggtttta tccacaaatt atttctctat attttctgtc
    27001 tattcaaaat aaacaataat tttccggcac aattatttgc tgttttttac cataacatag
    27061 tacgatcaac gtagaaaagg agatgttttt atgaaagttg ttgtcattgg cggtgtagct
    27121 ggcggtcctt catttgccac tcgtttccgt cgattgaatg aagcgcacga aatcattatc
    27181 tatgaacgcg gagaaaatat ttcttatgca agttgtgctt tgccttatta tttaggaggt
    27241 gtgatcactg accgtgactc gctgatcgaa cgtacaccag aaatattgaa aacaaaaaac
    27301 aacatcgatg tctttactaa acacgaagta acagcaatcg atccttctac taagcgatta
    27361 acagttaaag acctatccac aaatgaagaa ataaaaacag attacgataa gttgatcatc
    27421 tcttctggtg ctagaccaga ttatccggat attcccggag tttttgaagc agaaaacggc
    27481 tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat cgttccttga agaaaaaaat
    27541 ccacaacatg tcgtcattct tggtgcaggt gttatgggtc tggaattagc tgagaatttc
    27601 aagcatcgcg gcttagacgt gactttaatc gatcaattgc cacaagtcgc tttcccttat
    27661 gatccagaaa ttgctaattt agtttatgac aaattgctga aagaaggatt agccgttcat
    27721 ttagaaacaa gagttactga gatccgtgat aaaggtcgag aaatagtatt atcagatggc
    27781 tccgtccttt ctgctgatat gctaattttt gctgttggtg tttctccgaa gaatgaagtg
    27841 gtgaaagcaa ccggcataca attatcagat acaggtcaga tcattgttga tgaccagtta
    27901 caaaccaatc tgccggacat ctatgcgatt ggcgatatta tcgaaacgac tagtgtagtg
    27961 actggtcagc cgatccaaag tatgctttcc agtgcggcca atcgtcaagg acatatgctg
    28021 gcagatattt taaatggtac gcctatgcgc tatcgcggat atattggtgc aggtgtcgca
    28081 aaaatctttg atcatacagc aagttatgcg ggaatgacag aacatgaatt aaaagcatca
    28141 ggcataacaa attataaaac tgtttttatc actccctttg accatgccta tttctatcca
    28201 gaagctacaa gattaaatct aaagctgatt tttgatgcag atagcggtcg tattttaggt
    28261 ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg gagaactttc tgtagcaatc
    28321 accggaaact tgacagtctt tgatttgcct gatttggagt tgccttactc tccaccgtat
    28381 tctactaccc gtgacccgtt gaatatagct ggttatgtcg caatcaatca aatgacgaat
    28441 atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg atttgaaaga agcgttcttt
    28501 ttagatatac gtgaacctaa taaagcacct tctggcagta tctcggcaac aaaaaatatt
    28561 ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa aagataaaaa agtttacatt
    28621 actttcagaa gaggattgaa tacttatact tctgcccgaa tcttggcagg tttgggtatc
    28681 aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg ggtagccgcg gtctaggagt
    28741 tatttttact tgaggagtaa aaatgaaaaa gtatataggg ttgttggttg gtatgtatat
    28801 agaatagaag ataaatgtga agaaattgtg cattatttct tcacatttat taggttatag
    28861 tttaagaaag tttaaacatt gcgcaattta attattttag ctgtttcttt tcataaaaaa
    28921 acatgttgtg actctgagac aaatcatagt ccatagtgat gtctctagag taactacgcc
    28981 taatattacg aggcatcata ttaatctatc agcactagta cctaaactag catccctttc
    29041 gaatattttt tcttatataa taaagtttgc tttcactcct ctaatctcac caaatcgact
    29101 ggcttgcgtt gtgtgatact aggaactgat tggtttagaa cgacttttct attgtaatca
    29161 tatttttcgc catcctcgtt gtcttctaat ccttcaatga atgatcccta atgtgtgtat
    29221 taaataattt aatttttttg ttcctattta tttaa
//
LOCUS       DACEDO010000034        28130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEDO010000034 DACEDO010000000
VERSION     DACEDO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.034"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            196..2730
                     /gene="secA"
                     /locus_tag="IWO52_001518"
     CDS             196..2730
                     /gene="secA"
                     /locus_tag="IWO52_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0355792.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2856..3957
                     /gene="prfB"
                     /locus_tag="IWO52_001519"
     CDS             join(2856..2918,2920..3957)
                     /gene="prfB"
                     /locus_tag="IWO52_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0355793.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4062..4748
                     /gene="ftsE"
                     /locus_tag="IWO52_001520"
     CDS             4062..4748
                     /gene="ftsE"
                     /locus_tag="IWO52_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0355794.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4732..5625
                     /locus_tag="IWO52_001521"
     CDS             4732..5625
                     /locus_tag="IWO52_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355795.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5777..7225
                     /gene="cls"
                     /locus_tag="IWO52_001522"
     CDS             5777..7225
                     /gene="cls"
                     /locus_tag="IWO52_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0355796.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7562..8419
                     /locus_tag="IWO52_001523"
     CDS             7562..8419
                     /locus_tag="IWO52_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0355797.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8534..9454
                     /gene="pstC"
                     /locus_tag="IWO52_001524"
     CDS             8534..9454
                     /gene="pstC"
                     /locus_tag="IWO52_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0355798.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9454..10338
                     /gene="pstA"
                     /locus_tag="IWO52_001525"
     CDS             9454..10338
                     /gene="pstA"
                     /locus_tag="IWO52_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0355799.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10353..11159
                     /gene="pstB"
                     /locus_tag="IWO52_001526"
     CDS             10353..11159
                     /gene="pstB"
                     /locus_tag="IWO52_001526"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355800.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11175..11933
                     /locus_tag="IWO52_001527"
     CDS             11175..11933
                     /locus_tag="IWO52_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355801.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11946..12623
                     /gene="phoU"
                     /locus_tag="IWO52_001528"
     CDS             11946..12623
                     /gene="phoU"
                     /locus_tag="IWO52_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0355802.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12861..13892
                     /locus_tag="IWO52_001529"
     CDS             12861..13892
                     /locus_tag="IWO52_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0355803.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13889..14656
                     /locus_tag="IWO52_001530"
     CDS             13889..14656
                     /locus_tag="IWO52_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0355804.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14756..16324
                     /gene="liaX"
                     /locus_tag="IWO52_001531"
     CDS             14756..16324
                     /gene="liaX"
                     /locus_tag="IWO52_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0355805.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16941
                     /locus_tag="IWO52_001532"
     CDS             16624..16941
                     /locus_tag="IWO52_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0355806.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            16941..17291
                     /locus_tag="IWO52_001533"
     CDS             16941..17291
                     /locus_tag="IWO52_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0355807.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17458..18396
                     /locus_tag="IWO52_001534"
     CDS             17458..18396
                     /locus_tag="IWO52_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0355808.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18411..19241
                     /locus_tag="IWO52_001535"
     CDS             18411..19241
                     /locus_tag="IWO52_001535"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0355809.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19285..20310
                     /locus_tag="IWO52_001536"
     CDS             19285..20310
                     /locus_tag="IWO52_001536"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0355810.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20325..21263
                     /gene="galU"
                     /locus_tag="IWO52_001537"
     CDS             20325..21263
                     /gene="galU"
                     /locus_tag="IWO52_001537"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0355811.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            21388..21828
                     /locus_tag="IWO52_001538"
     CDS             21388..21828
                     /locus_tag="IWO52_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0355812.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21831..22433
                     /locus_tag="IWO52_001539"
     CDS             21831..22433
                     /locus_tag="IWO52_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0355813.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(22496..23599)
                     /locus_tag="IWO52_001540"
     CDS             complement(22496..23599)
                     /locus_tag="IWO52_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0355814.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            23809..24810
                     /gene="ccpA"
                     /locus_tag="IWO52_001541"
     CDS             23809..24810
                     /gene="ccpA"
                     /locus_tag="IWO52_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0355815.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            25165..25560
                     /locus_tag="IWO52_001542"
     CDS             25165..25560
                     /locus_tag="IWO52_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355816.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            25560..25811
                     /locus_tag="IWO52_001543"
     CDS             25560..25811
                     /locus_tag="IWO52_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355817.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            25854..26249
                     /locus_tag="IWO52_001544"
     CDS             25854..26249
                     /locus_tag="IWO52_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355818.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            26246..27502
                     /locus_tag="IWO52_001545"
     CDS             26246..27502
                     /locus_tag="IWO52_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ0355819.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            27514..27837
                     /locus_tag="IWO52_001546"
     CDS             27514..27837
                     /locus_tag="IWO52_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355820.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            27830..28096
                     /locus_tag="IWO52_001547"
     CDS             27830..28096
                     /locus_tag="IWO52_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355821.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         9453 a         4832 c         6082 g         7763 t
ORIGIN      
        1 acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct cttttagatg
       61 aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc aattatctat
      121 aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca gaaggaaaag
      181 gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga taagaaagaa
      241 ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca aatggcagca
      301 ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata ccaaaaagga
      361 gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc agcaaaacgt
      421 gtattggggt tatatccaca ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga
      481 aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat gccagtatat
      541 ctaaatgctc tgactggcga aggggtacac gtagttaccg tcaatgaata tttagcaacc
      601 cgtgactcga cggaaatggg cgaactttat aatttcttag gattaacagt ggggttgaat
      661 atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgtgatat cacttatagt
      721 acgaataatg aattaggatt tgactacttg agagacaata tggtcgttta ccgccatcaa
      781 atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat cttgattgat
      841 gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatccacagc tttatataca
      901 agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat cgatgtacaa
      961 tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac attcggtttg
     1021 gataacttat acgacattga aaatactgca ttgacccatc acttggatca agcattgaga
     1081 gccaactata tcatgctttt agatatcgat tatgttgtcc aagacggaaa agtactgatt
     1141 gttgaccagt tcactggtcg gatcatggat ggacgccgtt attcagatgg acttcaccaa
     1201 gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc
     1261 actttccaga actatttccg tatgtacaag aaacttgcgg gaatgactgg tacagctaaa
     1321 acagaagaag aagaattcag agaaatttat aacatccaag tataccagat tccgacaaat
     1381 cgtccggtta tccgtgatga tcgtccagat ttgctttacc caacattaca aagcaagttc
     1441 cacgctgtcg tgcaagatat caaagatcgc taccgcaaag gtcaacctgt gttagtcgga
     1501 actgttgcgg ttgaaacatc tgagttatta tcagatatgc tgaatcaaga acgtattcct
     1561 catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag agatcatcat gaatgctggt
     1621 caaaaaggtg ctgtgacgat tgctaccaac atggctggtc ggggaacaga tatcaagtta
     1681 ggacttgggg taagagaact tggtggtcta gcagttatcg ggacagaacg acatgaatct
     1741 cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca
     1801 cagttctact tgtcattgga agatgatttg atgaaacgat tcggttcaga acgaatcaaa
     1861 tctttcttag atcggatgaa gatcggtgag gaagatgctg ttatccaaag caagatgctg
     1921 acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa ataactatga cacacgtaaa
     1981 aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga
     2041 caagaagtaa tcatggaaga aaaaagctta tccaagccac taatgaacat ggtcaaacgg
     2101 acaatcagtc gtgtagtaga tgcgcataca caattagaga aagaaaattg gaatttggaa
     2161 agtatcatcg attttgccgg cagtactctt gtccatgaag atagtattac tcttgcagat
     2221 atcgaaggaa aaacagctca ggagatcaaa gagtatctaa acgaacgagc acaagaagta
     2281 tttaatacca aagcggctca actaaatggt ccagagcaac tgttggaatt tgaaaaagtc
     2341 gtgatcttgc gtgttgtgga ctctaaatgg acagaccata ttgatgcaat ggatcaatta
     2401 cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc cattagtcga atatcaaaca
     2461 gaaggctata agatgtttga agatatggta ggcgccatcg agtatgaagt cactcgaatc
     2521 tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac aggtttctca aggaacagct
     2581 tctcactcag aagacggaga tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca
     2641 atccatgtga acaaggtcgg aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat
     2701 aagaactgtc atggaaaaaa tgcaaactaa tttctgacag caggattggt gagacaaatt
     2761 ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt tagactagat
     2821 ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa attcgaaatc
     2881 tcctgacaga aatgcaggaa aaggtcacaa gcttcaggag gtctctttga cttagaccag
     2941 ctggaagaag acattgcgga agcagaagca aaaatggctg aacctggttt ttgggatgat
     3001 agcgaagctg ctcaaaaagt gatcagtgaa aacaatgcaa ataaagaaac ttatgatcaa
     3061 ttccattctc ttgctgaaga actggaagaa atcgaattga tggtggagat gttgcaggaa
     3121 gaacctgatg cagccatgca agaagaagca gaaacacgta tcaatgcgct agatgaaaaa
     3181 atgaagacat atgaattatc tatgttgtta gatggtcctt atgatcgaaa caacgccatc
     3241 gttgaattgc atccaggggc tggcggaacg gagtcccaag attggggcag tatgctttta
     3301 cgtatgtata cacgctgggc agagcagcat gggtatcaag tagagacact agattatcaa
     3361 gcaggtgatg aagcaggaat caagagtgtg accctactga tcaaaggaca caacgcattt
     3421 ggttatctga aatcagaaaa aggtgttcat cgccttgtac gtatctcgcc ttttgactca
     3481 gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct tgatgaaacg
     3541 atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc aagtggtgca
     3601 ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt gcctacagga
     3661 acagtagtag ctagtcaggc acaacgttca caattgaaaa accgtgagca agcgatgggg
     3721 atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga agcagctgca
     3781 cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta tgttttccat
     3841 ccatattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt ccaagcagtt
     3901 atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct acaataagaa
     3961 cccattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa cattgccatg
     4021 ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa atgaaggatg
     4081 taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcaatt gtcatcgaac
     4141 aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca ttcatcaaat
     4201 tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa
     4261 tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg gtgttccaag
     4321 actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta
     4381 tcggcagaaa acctcgggat atcaaaaaac gagtaatgga agtgctggac cttgttggtt
     4441 tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag caacgtgtct
     4501 ctattgctcg ggcaatagtg aatacgccta aagtacttat cgcggatgaa ccgacaggaa
     4561 atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc aatgcccaag
     4621 gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt cggcaccgag
     4681 taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa tatggatacg
     4741 atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa acgtaacggc
     4801 tggatgacag ttgcttcagt cagtgcggta acgatcacat tgactttagt cggtgtattc
     4861 atggcagtta tcatgaacgc aactaaatta gctcaagata tagaaggaaa cgttgatgtc
     4921 tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga aaaagaattg
     4981 aatgacttag atcatgttaa aaaagttgaa ttttcaagca aagaacagga attgaaaaaa
     5041 atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa cccgttatat
     5101 gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc agaagaagca
     5161 gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga tcggattttc
     5221 caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct gctgttcgta
     5281 gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca gcgtgaaatt
     5341 caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt tttccttgaa
     5401 ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac atttggttat
     5461 gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc attgattcat
     5521 ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt gatcattggt
     5581 tcacttggct cgatcatttc tatgagacgt ttcttgaaat tctaatacca caatataaat
     5641 aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct aggagaatag
     5701 aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt aagttggtag
     5761 aagaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc ttttgattat
     5821 gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg
     5881 ggcatgggtg ctcgttttga tgttcattcc agttgtagga tttattttat atattttttt
     5941 aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga aaatcggtaa
     6001 gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc ctcatccgcc
     6061 tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg aaagtacgct
     6121 ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat tcgatgcgct
     6181 gattgaagat atcaagcagg cagaagacca tatccacttc cagtattata tttatcgcag
     6241 tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac gaggggtaaa
     6301 agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct tttttcagaa
     6361 tttgaaaaaa gcaggcggag aagtcgcatt tttctttcct ctatttgttc cgtatattaa
     6421 tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga aaatcggata
     6481 tacaggtggg ttcaatgttg gaaatgaata tctaggattg gtaaagaaat ttggttattg
     6541 gcgagacaat catttgcgta tttacggaga agcggtgtat accttgcaaa accgtttttt
     6601 gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat tttttccgtc
     6661 cattcactct actggtgata tagctgttca aatcgtgaca agcggaccag ataccgaaca
     6721 tgaacaaatc aaaatgactt atttgaaaat gatttcgtta gccaaacggg agatattgat
     6781 ccaaacgcct tattacatac cggatggatc gattcatgaa gcactgaagt tggctcttct
     6841 ttcaggcgta aaagttcata tccaaatacc taacaaacca gatcatcttc tcgtttactg
     6901 ggcaacgtat tctttcgctg cagagctaat cgagtatgga gcaaggatcg aaacatatga
     6961 gaatggattt attcatgcaa agacgatgat tatcgatggt ggcattgttt cagttggctc
     7021 agcaaatatc gatgttcgtt catttcgctt ggactttgag gtcaatacac tggtttatga
     7081 tgaacgaatg gcttcacgtg taagaaaagc tttttttgaa gactcgaaaa tttctacgca
     7141 tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc aagatgaaag aaggattagc
     7201 aagattgatt tcgccgctcc tttaaaacat aaaaaggaca tgagaaccat caacagatgg
     7261 agatctcgtg tccttttcta acggggccat ttctgagagt acgtcagaaa tggagaaagc
     7321 gtctcgcatg agcgaaatta actttggggt atctcttgtt ttttggggaa agcaagaaat
     7381 aagctaatat atgctattaa aagagacaca tttcaaagcc agtatagcat agaaactttt
     7441 aatgagatag ttttttttac aattatgaga atttattttg ttatttaatg attttgattc
     7501 gttttcatca agtaatgaaa atgataaaat aaacatactt taggattaaa ggagatttct
     7561 aatgaaaaaa cttaaattat tcttaccaat aataggatta gctattcttt tatcaggctg
     7621 ttcaaagtgg attgatcgag gagaatccat tacggctgtt ggctcttctg ctttacagcc
     7681 tttagtagaa acagttgctg aaacttacca gacagaaaat cctggaaaat tcatcaatgt
     7741 acaagggggc ggaagcggaa ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg
     7801 aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat gcctcagcat tagtcgatca
     7861 tcgagtagca gttgttggga tcactcctat tgtgaacaag gatattggcg taaaagacat
     7921 cagtatggaa aacctgaaaa aactatttac aggcgagatc aaaaactgga gtgaagtcgg
     7981 tggaaaagat cagccagtag ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac
     8041 gtttgaaaaa tgggtattgg acggagaaac agctgttcag gcacaagaac aagattcaag
     8101 cggaatggtg cgccagatcg ttgccgacac acccggtgca atcagctatg ttgctttttc
     8161 ttatgtttcc gatgatgtag atacgttgaa tgttaacggt gtagaaccga cagatgaaaa
     8221 tgtgacaacg aacaaatgga cgatttgggc atatgagcat atgtacacaa gagatcatcc
     8281 aaaaaaactg acaaaagaat ttttagacta tatcttatct gatgaagtac aagataacat
     8341 cgttggagaa ctaggttata tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg
     8401 aaatatcgtt aaagagtaat ctttacacaa aatttacaaa cttagctaaa cttttacatg
     8461 ttattaaaac caaattgatg ttcaatttgt acactgagtg agtatgttaa acctgattaa
     8521 gggggagtca tctgtggaag atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa
     8581 aatggaacag cgtggaaaat tcatcagttt tttatgtatc gcgctgatcg tgttggttgt
     8641 attatctatt ttttattttg tagctagtaa aggattggcc acctttttta ctgataagat
     8701 caatgtattt gattttctgt ttggtacaga ttggaatcca agccaaatcg gagcaaatgg
     8761 gaaaccaatg gtaggggcat tgcctatgat cacaggttcg tttattgtga cctttttatc
     8821 tgctatcgtt gccacgccat ttgccattgg tgcagcagta ttcatgacgg agatctctcc
     8881 taaaaacgga acgaagatct tacagccggt catcgagctg cttgtaggta ttccttctgt
     8941 cgtttatggt tttatcggtt tatcagttat cgtgcctttt gtcagaagta tttttggcgg
     9001 gactgggttt ggtatcttag ctggtacatt tgtgttgttc gttatgatct tgccgactgt
     9061 cacaacgatg actgttgatg cattaaaggc agtgccacgg cactatcggg aagcttcatt
     9121 agcgcttggt gccacaagat ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc
     9181 cgggatttta acagcagtcg tttttggtat ggctcgtgcg tttggtgaag ccttagctat
     9241 ccaaatggtc atcggtaatg cagcattgat gccaacaggt cttactactc ctgcttctac
     9301 attgacgtcc atcttaacaa tgggaatcgg aaatacaatc atgggaacag ttgaaaataa
     9361 tgtgttatgg tcattagcgc tgatcttgct attgatgtca ttattcttta atatcgtgat
     9421 ccgtatgatt gggaagaaag gagagctgaa ataatgaacg caaaacgctc agataaaatt
     9481 gctacaggtg ttttatatat agtttcgggg atcatcgtat tgattttagc agctttacta
     9541 ctttatatct tagttcgggg aataccgcac atttcatggt ccttcttgac cgaaccttcc
     9601 aaagcctacc aagaaggcgg tggaatcggg atccagctat ttaactcgat ttatttgctt
     9661 ttgatcacga tgatcatcag ttttcctatt tctttaggtg cgggtatcta tctttcagaa
     9721 tatgctggaa aaaactggct aacagatgtg attcgcacgt cgattgaaat cttgagctcg
     9781 ttgccttctg tcgttgtcgg tttattcggt ttcttggttt tcgttattca attccagtat
     9841 ggtttctcga ttatttctgg tgcattggct ttgactgtat tcaacttgcc tcttttgact
     9901 agaacagttg aggagtcttt acaagcggtc cattacacac aacgagaagc aggattggcc
     9961 ttaggtttgt cacgctggga aacagtgata aaagtggtgg ttccagaagc gacaccaggg
    10021 attttgactg gtgtgatcct aagttctgga agaatttttg gtgaagcagc tgcattgatc
    10081 tatacggctg gacaaagtgc acctgcgtta gattttacga actggaatcc attaagtgtt
    10141 tcaagcccga tcagcatttt ccgtcaagct gaaacattag ctgtccatat ttggaagatc
    10201 aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg
    10261 attattgctg ttttactgtt caacttcggc gctcgtgcga ttggcaaacg attataccga
    10321 aaaatgactt cagcctaagg agaagaagca aaatgaaaaa atataactgg aatgaaacaa
    10381 atgtcatcac catgccaaaa gagaaagaag tagcactata tacggatgat ctacatgtct
    10441 ggtatggcga caatgaagca atcaaaggag tggatctgga atttgaaaaa aataagatca
    10501 ctgcactgat tggtccttcc ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa
    10561 tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat catgtatcaa ggcgtagata
    10621 ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg gattggcatg gtttttcaac
    10681 gtcctaatcc gttcagcaag tcgatttatg acaatattac atttgctttg aaacagcacg
    10741 gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct
    10801 tgtgggacca agtaaaagac agcttggaca aaagtgcctt ggctttatct ggcgggcaac
    10861 agcaacgttt atgtattgct cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg
    10921 agccggcaag tgctttggac cctatttcga caggaacggt tgaagaaaca cttgtaaact
    10981 tgaaagaaga ctatacgatc atcatcgtga cgcataacat gcaacaagca gctcgtatca
    11041 gcgactatac tgcctttttc tatttaggaa aagtaatgga atatgacaaa actaacaaga
    11101 tatttaccag accaaaaatt caggcaaccg aagattatgt gtccggtcat tttggttaag
    11161 acggaggcct caacatgaca aaagagatta tttcatctaa agatcttcat ctgtattatg
    11221 gaaagaaaga agcactaaaa gggatcgatc taagcttcaa tcaaggggaa atcactgcga
    11281 tgatcggtcc ttctggttgc ggaaaatcca cttatctgcg ttctttgaac cggatgaatg
    11341 acctgattcc gaatgtaacg atcaccggaa gtgttttata taaagacaaa gacatctatg
    11401 gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg catggtattc cagcagccaa
    11461 acccatttcc tttttctatc tatgaaaatg tcatctatgg attgaaatta aaaggggaaa
    11521 aagataaaaa agtccttgat caagtagttg aagaaagcct gaaggcagcg tctgtttggg
    11581 atgatgtgaa agacaaattg cataaaagtg ctttatcttt atccggtggg cagcagcagc
    11641 gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat catcttattg gatgaaccaa
    11701 caagtgcgct tgatcctgtt tctactggga aaatcgaaag tatgctgtta gaattaagag
    11761 agcggtatac gatgatcatc gtgacccata atatgtctca agcttcacgt atttcagata
    11821 agacggcatt ctttttagat ggtcacttga tcgaatacaa tgatacgaag aagatattca
    11881 tgaatccaga aaaacaagag acagaagatt atatttcagg tcgatttgga taaggaggaa
    11941 acggaatgct acgtacacaa tttgaagaag aattgctgaa tctccataat cagttctacg
    12001 aaatggggat gatggtgagc agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg
    12061 ataaaatctt agcccaagaa gttatcgaaa acgatataaa tatcaataac atggaaacac
    12121 gcttggagaa gaaaagtttt gaaatgatcg cattgcaaca gccagttaca actgatttgc
    12181 gtatgatcat tacggtcatg aaagctagct cggatttaga acgtatggcc gatcatgcgg
    12241 tgtctgttgc taaatccacg attcgcgtaa aagggcagac gagaataccg gaaatcgaaa
    12301 aagagatctc cgatatgtcc gattatgtga aaaaaatggt ggacaacgtc ctagttgctt
    12361 atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa tatggatcaa agagtcaatg
    12421 actatttcaa tcgaatctat aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag
    12481 tcatcagtgg aacagactac ttgaatgttg ccagttatct agaacggatc ggagactatg
    12541 tgacaaacat ctgtgaatgg atcgtctact tggctacagg gaaaatcact gagttaaata
    12601 cgaaccacaa tgagattttc taaactgaag ctgtgattat gccgtttgtt taggaaaaga
    12661 ggttgtgaaa tcagcggttg tgaaaaagct gttctgcatc aagtaataag aacagtcaaa
    12721 caaaaaatag ctcctcatat ttttcgtttg gctggactta ttaccgcaga tgcaagcttt
    12781 tgaacaccgt ttattcggga aagggtttgt gacaaaacat gtcacaaacc cttttcatca
    12841 tttcatagaa aggataaaga atgcaaaaaa aaattagcat catcactata ttttttattt
    12901 gtgtgcttac ttcatgggtt attcagcatc aagccgcttt atatgaagta cctgtcatcg
    12961 agatagaaaa agtacgaata aaagaggatc atcaattgat tacaggaaaa ctgatgaaca
    13021 cggatcaaaa aggagaatcg atttatttat cggttcctta tcaaaaaaat caaatagaaa
    13081 cagcaaatat aaaagcgaga caaatgtatc tagtccaaat gcatggagat gaatggcagc
    13141 tagtagcaaa aaaaaatgat ggctggcttt tcttcttcac aagtatcttc attggcgcca
    13201 tgttgcttat tggtggaaaa cagggtctat ttgctttagg aagtctcatc atcaatatcg
    13261 ttttactttt acttttgttg ctcatcttta ctaaaacaga agggatttct ctattaaata
    13321 tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta gatggatgga
    13381 atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta gcatttttta
    13441 tttgtctttt gacaatgaat cattggaaag accaaggact gcgctatgag gaactgaatt
    13501 atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga acagctggag
    13561 gaacactcga taccgttatc acagtcattg cgacattaga agaattgact aagcgacaac
    13621 cagaaataac agcgaaacag ttatggcttt caggaaggca agtgggtcga gatgtgattg
    13681 gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca tcgcttcttt
    13741 tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta tcattggaat
    13801 ttttacgcgt cattgctggc agcttaggaa tagtattatc gatccctatc tcggtcctct
    13861 tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg ttgggatttt
    13921 agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt taggcttatt
    13981 catcaattta gggatctttt tcctgttgtt attcggttat catcagaagt ggccgatact
    14041 ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt ttatcaacgg
    14101 ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat tttgtttcct
    14161 actattgctt cctatcactg attttttagc aatccaaggt tttacatcgg tagaactaga
    14221 agaattatct ggcttagata agactttagc gattgatttt agattactta cgagatcgtt
    14281 gcttctgatt agtcttagtg gtgctgtttt agatgcgagt gtagcaattt ctagtgggac
    14341 attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc attctagttt
    14401 cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg cattcatggg
    14461 aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc agcaagtgat
    14521 caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat tgtccgcact
    14581 acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa agaaagaaca
    14641 actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg aatcagtgca
    14701 taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa tttccatgaa
    14761 agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg aagaaggctt
    14821 agatttatta gaaagcatgg caactgaaaa agacgaaaag caaatcaaaa aagaagcaga
    14881 caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg ataaacttga
    14941 aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa gacaagatca
    15001 agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa cttcggcgcg
    15061 tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg agaaacagga
    15121 agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag aagaattagc
    15181 acagcgtcag acgttagaag cagaaatcaa agaattagaa gctagtggcg atacactgtt
    15241 agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc aatggtctga
    15301 gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag atcaagcaac
    15361 agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt taggcaaatt
    15421 cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg aatggaaaga
    15481 tgtcagctta cgtgtccctg ggattgcgac aacgaaattt gagcatgaat tttactatga
    15541 agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga cgttaaaaac
    15601 gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa aaatgggtgc
    15661 tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag atcatatctc
    15721 cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac ctaaacgagt
    15781 atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa cattggaagc
    15841 taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat taactgcaac
    15901 gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga acatcttaga
    15961 ttctattatc gaaacagtaa atggtacagt gacatttggt gcgacagcag aaaacctttc
    16021 tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt tgaaaaaagt
    16081 agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca tcggtttgga
    16141 aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt acgaagtagt
    16201 tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt cagatggtga
    16261 aatcgcgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag atacagataa
    16321 ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt ttgtaaaata
    16381 gcttccccta ttttacaaaa ttgtgcctta ttgccgatag tctatctctt gaacaccgtt
    16441 taccaagaga ttatgaacct gccaattatc gccgagtcac aaggaataat ttcagaaaaa
    16501 gctcgtcacg ttttatgaat atggtaattt gtgaccgaga attttgcaga aagacaccga
    16561 ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca gaggtgagac
    16621 atgatgaaaa aaaagttgac aaaatctaat aaaaatgttg ttttgacagg gactttagca
    16681 ggaataggag aatacatcgg gattgatccg actgttatcc gtgtgcttta tgtattcctg
    16741 agcttagttg cttttggaag tccaattatt ttatacattt tattagcttt gattatccca
    16801 tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga aaaaaacaat
    16861 tatcgtgaac aaactcgtga acaaacaaaa aaacgcaaag aagcggaaaa aatcgatgat
    16921 gacgaatgga gtgactttta atgtcgtact ttcaacgtat tttagttaat acgttaacat
    16981 tcatatcatt ggctgttctt tttcctgcga tggtccatgt gagcagtatt tggacggccg
    17041 ttcttgccag cttcgtattg tctgtgttga atatgtttgt caaaccgatt ttgacgatac
    17101 tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc aatgcagcga
    17161 ttcttaaaat gacctccttt tttgtgggag cagaacattt tagtttttct tcattcggtg
    17221 cagcgttgtt agtagctatc ttgatgtcgc ttgtcaatgt aattgtcagc gaacataaca
    17281 gtagaaaata aaagaaagct gtgccgaact atcggcgcag tttttttatt tagaatcagg
    17341 aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt ggtagtactt
    17401 ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt agcatgaatg
    17461 tcagaagttg taaaagttag ccaattagtg aaaaaattat ctttggaaat cgtgacaggc
    17521 gacgagaaaa gcttagaccg tgtcatcgta actggggaca tctcacgccc aggactagag
    17581 ttgactggtt attttaatta ttattcccat aatcgtttac agctatttgg cagtaaagaa
    17641 atcagttttg cggaacggat gatcccagaa gagcgtttga tcatcatgag aagattatgt
    17701 agtgaagata tgcccgcatt tattatttca agaggattac cagctccaga agaaatgatc
    17761 caggcagcaa atgaaaatgg gatagctgta ctgcgttctc ccatctcaac ttctcgttta
    17821 ttaggagaat tatccagtta tttagatggt cgccttgccc ctcgaacaag tgtccacggt
    17881 gttttagtgg atgtttacgg attaggtgta ctgattcaag gtgacagtgg gataggaaaa
    17941 agtgaaacag cgttggagtt gatcaaacgc ggccatcgat tgatcgcaga cgatcgagta
    18001 gatgtttatc agcaagatga attaacggtg atcggagaac cgcctaaaat cctggaacat
    18061 ctgattgaaa tccgaggaat cgggatcatt gatgtaatga atctttttgg tgcgagtgcg
    18121 gtacgtggct ctatgcaagt ccagttggct gtttatctgg aagcatgggc aaaagataag
    18181 aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt gggcattcca
    18241 caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga agtagccgcg
    18301 atgaatttcc gtgcacgtac aatgggcttt gacgcaacga aaacatttga agaaagatta
    18361 agtcgcttga ttgaagaaaa ctcaggcaac gactagcaag gagtgacaat atgttagcac
    18421 aaatcaatcg agttgctttt gagcttgtag ggattcctat ctactggtat gcattgatca
    18481 tcgtatcagg tatcattatc gctatgtggc ttagcagcag agaggctgtt cgggtaggat
    18541 taaaagcaga tgatgtgaca gattttatgt tgtggggatt gcctatctcc attattggcg
    18601 ctcgcttata ttatgtttta tttgatcttc cccagtatat agctgatcca atccaaatat
    18661 tcaatatccg ttcaggaggc ttagctattt atggcggact gattgcaggc gcgatcacgc
    18721 tgtatttttt tactaaatat cattttattt ctttgtggac ttttttagat atcgctgcac
    18781 caagtgtatt attagcacag gcaatcggtc gttggggaaa tttcatgaac cacgaagcat
    18841 atggaccaga cacgacgcga agcttcttag aaagtctgca tttaccgcaa ttcatcattg
    18901 ataatatgta tatcgaaggg atctatcgtc aaccaacatt tctttatgaa tcactttgga
    18961 gtttgattgg atttattttg cttttgtttt tacgaaagaa accacacctt ttgaaaaaag
    19021 gcgaagtcct tttatgttat ctaatgtggt atagtttcgg gcgtttcttt attgaaggat
    19081 tacgtacaga cagtctttac tttttaggtg tgatccgtat ttctcaatta ttatctgctg
    19141 tgttatttgt cggtacattg attctattta tttggcgcag aaaaaaagga aatttaccgg
    19201 attatgaccg ctcattagga aaaaatcaac aaattatctg atttttagag cgttaaaaat
    19261 gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg accaggctct
    19321 tggggtactg ctttggcaca agtcctcgcc gaaaacggac atgacgttcg gatttgggga
    19381 catcatgctt cacaagtaga agaaatcaac acccaacaca cgaatcaacg ttatttacca
    19441 gatcattatc tacccgattc catccaagca ttcaccaata tggaagatgc agtaaaagac
    19501 gcagatgcgg tgctattcgt cattccaacg aaagcaatac gttctgttgc aaaagaacta
    19561 gtgcctaaat tgaatacaaa gccagtgatc atccatgcaa gtaaaggatt agaacaggga
    19621 acacataaaa ggatctctga catcttgtta gaggaaatcc cagcagaaaa aagaaaagct
    19681 gttgttgttc tttctggacc aagccatgca gaagaagtag caaaacatga catcactacg
    19741 atcacagctg caagtacaga tgcaaaagca gcaaaatatg tacaagagct gttcatgaat
    19801 aattattttc ggatctatac caatcctgat gtgatcggtg tagagatggg ggcagctttg
    19861 aaaaatatta ttgctattgg tgctggcgct attcacggct tagggtttgg cgataatgca
    19921 aaggcagcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt agcaatgggt
    19981 gccaacccgc tgacctttat cggattaagc ggaataggcg atctagtcgt tacttgcaca
    20041 agtgttcatt ctaggaactg gcgcgcaggc aacttgttag gacaagggca gcccctagaa
    20101 gaggtgctag ccaatatggg aatggtcgtt gaaggtgtga atactaccca agcagcgatg
    20161 gaactagccc aaatgatgga aatcgaaatg cccatcactc aaacaatcta tgaagtgctg
    20221 tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga tggaaaaaaa
    20281 gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa gttaaaaaag
    20341 cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaaa gctatggcaa
    20401 aggaaatgtt gccaattgtt gataaaccaa caatccagtt catcgttgaa gaagcgttga
    20461 aatcaggtat cgaagacatc ttgatcgtta caggaaaagc taaacgacca atcgaagatc
    20521 actttgattc aaatattgaa ttagaaacaa acctaagtga aaaaggaaag accgaattat
    20581 tgaaattagt agaagaaacg acagatgtca acttgcattt catccgtcaa tctcatccaa
    20641 agggtttagg acatgccgtt cttcaagcaa gagcctttat tggaaatgaa ccatttgtcg
    20701 ttatgctagg cgatgatata atggaagacg aagtaccatt gacacagcaa ttgatcgatg
    20761 actatgagcg tacacacgct tctacgattg ccgttatgca agttcctcac gatgaaactt
    20821 caaaatacgg aatcatcaac ccaggggaag tactggaaga tgggttatac aatgtcaaaa
    20881 actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt attgggcgtt
    20941 acttattgac tccagaaatc tttgatgtat tatcagaaca acaaccaggt gctggaaatg
    21001 agatccaact aactgacgca atcgatacgt tgaataaaac acaacgcgta tttgcccgta
    21061 aattcacagg taaacgttat gacgttggtg acaaatttgg ttttatgaaa acaagtattg
    21121 aatatggtct gacacatcca gaagtagggg cacctttgcg tgaatacatc atgtctttag
    21181 gtaaaacatt agaagcagaa ggaagcaaga tcaatcataa tccaaaagga ataacagaaa
    21241 tcaaagaaga aaagaaaaaa taatcttttt ttctaacgag cttcagctgc taattgaata
    21301 aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta tccttataga
    21361 aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg attgatcgca
    21421 gcaatcgcct ttgcgatact tgtcgttttc atcgttcgcg tattgttgca agtttccaaa
    21481 actttagaaa aagtagacca gactgtcgca gaagcaaata cgacaattga gatcgtcaca
    21541 caagacgtag atcttttatc tagacaagta gaaggcttgc tagtcaaaag taatgagctg
    21601 ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc agttgctgat
    21661 ttaagtgaaa gtgtatcgga acttaatcat tctagtaaaa acatcgtgac gaaagtcggc
    21721 ggaatcggaa aaacgacagc taaagcgact gttgccggaa aagtcggtgg aacagcgatg
    21781 aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaga atggctaaaa
    21841 aaggtggatt tttattaggt gcagtagttg gaggtacagc cgcagcaatc gccgcattat
    21901 tgttagcacc caaatctggt aaagaacttc gggatgaatt ggctgctcaa gcagatgatt
    21961 tgatggacat ggcttcagat tacacagata tggcaaaaga aaaatcatct gagttaacag
    22021 gtattgcaaa agaaaaagcg caaggtcttt ctcaacaagc aggggatcta gcaggcaatg
    22081 ttaagcagaa ggtaagtgaa tcagcagagg aagcaggaag tgaatcagat gatatcttgt
    22141 taaacctcaa agaacaatca gctgatttat ctaatcgttt caaaaaaaca gcagaagatc
    22201 tgaaaaagcc agcagacgaa ttaggtagta ttgctaaaga tacttcagaa gatatcatga
    22261 tcgatgtaaa agatgctgca gataaagcag gagagacggt caatgaagga atgtcagaag
    22321 caaaaccagt tgcagatgac acaaaagaaa tgttgaaaat ggcaaaagaa gatgcaaaag
    22381 aagctaaaaa agaaatgaaa gatacaatga cagattcgac taccaatcac taagattact
    22441 catttctaga ataaaaaagg tgagagcgga ccagctctca ccttttttat tttcattact
    22501 ctaaaacaag taattctttt ggtgtagtcg tgaatggcac acaaccatca cttgtcacat
    22561 acacacaatc ttcgatacgt acccctactt tttcaggaat atagattcct ggttcaagag
    22621 agaagcacat cccttcttca atcactaagt cgtttccttc tacaagagaa gggaattcgt
    22681 ggacggttgt tccgattccg tgacctaaac ggtgattgaa atattcgccg tagccatagc
    22741 tgttgatcac gtttcgtgcg atttgatcaa gttcacctgc tgtcacacct gggcgaactg
    22801 cctcttgtgc ggcaagctgt gcttcaagag tgatgttata gattttttct tggaactcag
    22861 ttggtttttg ataagcaact gtacgagtag catcactgca atagccattc caaataacgc
    22921 caagatcgaa taacactagt tcatttggtg aaaccttcgt gcttcctggt gtaccatgag
    22981 ggcttgcagc atttgctcct gcaaggacta atgtgtcgaa agacatttgt gaaacacctt
    23041 gtttttttaa ttggtattcg atttctgcga taattgcttg ttcagttacg ccttctttta
    23101 ctgttttgaa gccaatttca aaggcaacat cggcccattt accagcttcg atcagtttgt
    23161 ctttttcttc taccgttttg atcagttgca gacgctggat aatagaagtt agatcggtgt
    23221 aagaatctgt acctgtcaag atatcagaga tagcatcgaa gcgatcgaca gtcagagcat
    23281 tcttttcaat acctacatta ccaggttgcc catagcgtgc gcgaagctct tttccgatga
    23341 tctcccaagg attttcgctg tctaagtagc cacgtacatc gtacgcccag ctgctgtttt
    23401 gagcatcttc aacttccaga gcaggagtga aaaggaaagg atcttgttcc agagggataa
    23461 aaagagctag tacacgctcg tgaggatcac tttcatatcc ggaaaagtaa gcgatgtggc
    23521 cagggtcgct gatataagcc aattctactt ggtgttgtgc catccattga cgtagttcat
    23581 tgatttttac gttattcatt tggcaaccta ctttctaaat taattttgta ttcaataatt
    23641 gtaccactga ttgattaaaa gaccaacgtt cttaaaactg aaaaaagtaa aaattttcat
    23701 gaaaacagag ataaaacggt tgcaattgtt gagattattt gatatcctat tttagaaatg
    23761 taagacgtat tttcagaaaa catataaaat atgaatagga gaacacaaat ggaaaagcaa
    23821 acaatcacta tctatgatgt agcgagagag gcaaatgttt cgatggctac tgtttcacgt
    23881 gtcgtaaatg gcaatccaaa cgtcaaacca gcaacaagaa aaaaagtctt ggaagttatc
    23941 gatcgcttgg attatcgtcc aaatgcagtc gcacgtggct tggcaagtaa gaaaacaaca
    24001 acagttgggg taatcattcc tgatgtcagc aatatgttct ttgcctcttt agcacgtggt
    24061 atcgacgatg tggcaacaat gtacaaatac aatatcatct tagcaaactc agatggcaac
    24121 gatcaaaaag aagtcaatgt attgaataat ttattggcta agcaagtgga cggcgtgatc
    24181 tttatgggtc accatatcac agacgaaatc cgtggagaat tttcacgttc aaaaacgcct
    24241 gttgtactag ctggttccat tgatccagat gaacaagtag gtagcgtaaa cattgattat
    24301 gtgtcagcaa caaaagatgc agtaaataaa ttagcaaaaa gcggcaataa aaaaatcgct
    24361 tttgtcagcg gtgcgctgat tgatcctatc aatggtcaaa accgtctaaa aggctataaa
    24421 gaagcattaa gcgaaaacaa tctaccatat agcgaaggat tgatttttga ggcacaatac
    24481 aactttaaag atggtctgtc tctagttgac cggatccata atagcggtgc aacagctgtc
    24541 tatgtttcag acgatgaatt agccattggt atcttggatg gtttattaga ccgcggcgta
    24601 aaagtaccag aagaattcga aatcatcaca agcaacaatt ctttactgac agaagtagct
    24661 cgtcctcgtt tgacaagtat cacacaacca ttatacgatt taggagcggt atccatgcgc
    24721 ctattgacga aaatgatgaa taaagaagaa gtcgaagaaa aaacgattat cttgccttat
    24781 ggaatcgaag aaaaaggatc aactaaataa taaaagatca atcatttaaa ggttgttcca
    24841 aaagtggtca acttctggaa caacgtttat acgagaaagg gggttatgac aaaattttgt
    24901 cataacccct ttttttttgt gactttaaaa aaagagaaaa tggcggaaac aaaccttacg
    24961 tccatcgtat acgacacaat ctgtgcgtag atgctacatt ttgttcacta ctatcaaaat
    25021 tactttaaaa tgaaaacgtg ataaaaaaga aagtagaaaa gtagataagt aactctaaaa
    25081 aatccaaaaa aatccaggct cgattgcgta cttatcttaa aggagaaaca atccatcata
    25141 tataacccga aaggagactt ctatttgcct aagaaaagac gaaagaccat agtcatcatt
    25201 ttgaccattc ttactatagg aatcggaggg aaattttaca tggacaaaag agaaatgcaa
    25261 agacagaaag atttactcgc agtagaaaaa cagtccgtca aagttctgaa aaataccttt
    25321 gcggatatca aggaagtgaa gattgaaaaa tctgctcgaa atgaaatgac gggttcatac
    25381 cggatagtta ttctaatgac taataaacaa gatcaatcga tttatttttc ttatagtttt
    25441 tggaaagaaa gaaatgagat aggaagttac ggaatagttg atgaaaaaaa gcaaaaagaa
    25501 ggaaatacgc tcaataaagt taaagtaact tactctaatg gaaatgagga aagtatttaa
    25561 tgattactga caaacaatat agtgaattaa gtgatgctgt ttactggcta gatcctaaac
    25621 atagagacta tgtacctgaa atgcaagaaa atttaagttt taaaattaac ggcactatgt
    25681 ataaaatcct aaaaatcaaa aactccttcg acggcatgca ggcgatggct gtagcgccaa
    25741 tcgtccatag caaattggag aaaaatttca aaaataaaaa aattcctgca aactttcgcg
    25801 tacttaaata atgaaggtgt caatacttac ataaaaagaa aggaggcacg ttcttgtcaa
    25861 ggaaaataag aaaactcagc ttagccattt tattaatcgt catattaggg atcggaggga
    25921 aagtctatat ggacaaaaga gaagcacaaa ggcagaaaga tttactcgca gtagaaaaac
    25981 agtcagtcaa agttctgaaa aatacctttg cggatatcag ggaagtgaag gtagaagaat
    26041 ttaaaaaaaa ccctgttacc ggaagttatg gtgcacttgt gacaatgaca aataatgaag
    26101 ggaaatctgt ttattttgat tatagttttt ggaaggagag aaatgaactt ggtggttttg
    26161 gcattgagaa cagagcggta caaaagacag gagtcactat aaaaagagta aaagtaattt
    26221 tttctaacgg acaggaggag ttagtatgac aataaacgat aagcattaca atgatattag
    26281 tgagaaggtt tattggctag atcccaaata tccccgctac aatgagggat acaaaaagaa
    26341 ctctgttaaa gaatttgctg gtatggaatt tcaaatccta caaatcaaag actcattgga
    26401 cggcatgcag gcgatgtctg tagcgccaat caagaacggt aaagtggatc tttctcaagt
    26461 agtgattgct tttgcaggaa cgaacttaag tgattggaaa gatttggaaa cggatgcaag
    26521 aagtatcatg aatctctacg ggaatagacc aggagcaagt ttttctgaaa aagcaacaac
    26581 aaacaagatt ttatccgtta gtggattgaa aacagcgggg caaatcaatt cagcgattgc
    26641 atttgctaat gaagtaaaaa aggaatatgg agaagctgct atttctacta ccggccattc
    26701 attaggtgga ttcttagcat tatatgtagc agcagagaaa caatggaaaa atgtcggctt
    26761 caacgggccg gacccgtatg atctcttatc accagaagca aaaaaatggg tggaagaaaa
    26821 tcccggtatg ctcaccaatt accgaaatca cgccgatttt atcggtaatt ttggtggtaa
    26881 tggcacaggt gctgaagttc ggatcagtat ggaaacagca ttcttgaatc tgcttgcttc
    26941 ccacagttta acctcttgga agtttgatga aaatggaaag ctgattattc ctgaaaatga
    27001 aaacaatgtt aaagctcgaa aacaacagaa agaaaaccaa gcgacccagt tgttttccta
    27061 tcagttgcaa gaactgaaca acatacgtaa aaaactacga ttaagtggcg gccagctgac
    27121 agttaatgaa caaatttact tagatgacac acaagctagg ttagtagtgg aaaagttatc
    27181 ggcaaaaatg aaaagcgcta tggaagaagt tatccgcatc aatcaaagag gaatttttga
    27241 gctagagaaa aaatggcaag aaggcttgcg gcaagcacgt gttgtagccc cttcgttgac
    27301 tgaacaggaa atcatagaag cattagctag tgtgggcgcc acaaaagaaa acttagtcga
    27361 ttctgcgaaa gaagtctatc tgctacgaat caataaggca agacgtatgg gggaggcctt
    27421 tgatacattg gcgaaggaga tccaagacaa aatcaatgaa atcgtggcac gagatcgaga
    27481 attagcacag caattcaatt aaaggagaag tagatgaaac tagaagaatt ggaaagagat
    27541 tatcgaagtg aacgaagaca attagaagct caagaagatg aattgatcca ctttaagaat
    27601 caaggattac gattgatgga gcaagaggag cagcgactgc atcatgtatt aaagaactta
    27661 gctttagata atgagcctct gtttcaagca agacgaagtt attcgaatta tcaggagcat
    27721 tatcaacaag ccctacaagt agaacgacga aaactctatc agcagcaaga tgaactagaa
    27781 cgttcctatc gtcaaaaaat cagacaaata actatggaga aatcatctta tgaatgaatt
    27841 tttaatgcaa ttactaaaag aattagagaa aaatctacat cgatcaaatg tacgttcagt
    27901 tggtatgggg atatctcagc aaatttgggc agaaaaacga ttgacagatc aaaactacca
    27961 aatgactgca gaagaagctg ctcaagaatt ttctaaccga catgtacact tgaaaggatc
    28021 agtaaaaggg caatttgcag aaaaactaac tgaattattc caaagacaag aaaagaaatt
    28081 aaaacagttt cgttagtaaa gttatataga aaaattttgt gaaaatggtt
//
LOCUS       DACEDO010000035        27998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEDO010000035 DACEDO010000000
VERSION     DACEDO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.035"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..586)
                     /locus_tag="IWO52_001548"
     CDS             complement(5..586)
                     /locus_tag="IWO52_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0355822.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IWO52_001549"
     CDS             complement(769..1395)
                     /locus_tag="IWO52_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0355823.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IWO52_001550"
     CDS             complement(1417..2748)
                     /locus_tag="IWO52_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0355824.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IWO52_001551"
     CDS             complement(2903..3304)
                     /locus_tag="IWO52_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0355825.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IWO52_001552"
     CDS             complement(3390..3596)
                     /locus_tag="IWO52_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355826.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IWO52_001553"
     CDS             complement(3691..5193)
                     /locus_tag="IWO52_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0355827.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IWO52_001554"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IWO52_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0355828.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IWO52_001555"
     CDS             complement(5764..6381)
                     /locus_tag="IWO52_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355829.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IWO52_001556"
     CDS             6602..7975
                     /locus_tag="IWO52_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0355830.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IWO52_001557"
     CDS             8050..9162
                     /locus_tag="IWO52_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0355831.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IWO52_001558"
     CDS             complement(9234..10871)
                     /locus_tag="IWO52_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0355832.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IWO52_001559"
     CDS             complement(11039..11647)
                     /locus_tag="IWO52_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0355833.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IWO52_001560"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IWO52_001560"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0355834.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IWO52_001561"
     CDS             complement(13201..13953)
                     /locus_tag="IWO52_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355835.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IWO52_001562"
     CDS             complement(14094..15044)
                     /locus_tag="IWO52_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0355836.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IWO52_001563"
     CDS             complement(15045..15884)
                     /locus_tag="IWO52_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0355837.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IWO52_001564"
     CDS             complement(15881..16627)
                     /locus_tag="IWO52_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355838.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IWO52_001565"
     CDS             16865..18235
                     /locus_tag="IWO52_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0355839.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IWO52_001566"
     CDS             complement(18290..19186)
                     /locus_tag="IWO52_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0355840.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IWO52_001567"
     CDS             complement(19467..20144)
                     /locus_tag="IWO52_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0355841.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IWO52_001568"
     CDS             complement(20235..21140)
                     /locus_tag="IWO52_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0355842.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IWO52_001569"
     CDS             21315..22769
                     /locus_tag="IWO52_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0355843.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="IWO52_001570"
     CDS             22785..23816
                     /locus_tag="IWO52_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0355844.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IWO52_001571"
     CDS             23823..25010
                     /locus_tag="IWO52_001571"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0355845.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IWO52_001572"
     CDS             25166..26548
                     /locus_tag="IWO52_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0355846.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26675..26905
                     /locus_tag="IWO52_001573"
     CDS             26675..26905
                     /locus_tag="IWO52_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355847.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27105..27773)
                     /locus_tag="IWO52_001574"
     CDS             complement(27105..27773)
                     /locus_tag="IWO52_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0355848.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7792 a         5726 c         5147 g         9333 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtaacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaatgaaga
    26641 agaagcacgc aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat
    26701 actgacagaa gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga
    26761 atttggaaga ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta
    26821 ctttatgatt gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga
    26881 aacaaaaaaa tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata
    26941 agaaaggatt tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga
    27001 agaagcaact tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt
    27061 tttgagtaac aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg
    27121 taagattcat gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat
    27181 tcaacataaa tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc
    27241 catgcttcag tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca
    27301 agcaggatcg ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca
    27361 ttctctagga taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt
    27421 gctcgcaact gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg
    27481 tctttgatta gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg
    27541 acgatacgtt cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc
    27601 cctacatgga cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta
    27661 tagactaaat caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact
    27721 aataataaac cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct
    27781 ccccctaact atctgttaat taaattatat atgctttctc acgaacacgc atggaatata
    27841 caaggaagaa atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa
    27901 agttagactt ttttggagtg gagaaatcca ctcctttttt ttatgctcta aaatgatatt
    27961 tttacacaac cgttacggtt gttttacgaa attggacg
//
LOCUS       DACEDO010000036        27723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEDO010000036 DACEDO010000000
VERSION     DACEDO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.036"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(129..1403)
                     /locus_tag="IWO52_001575"
     CDS             complement(129..1403)
                     /locus_tag="IWO52_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0355849.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            1676..4147
                     /locus_tag="IWO52_001576"
     CDS             1676..4147
                     /locus_tag="IWO52_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0355850.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HANQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4250..5986)
                     /locus_tag="IWO52_001577"
     CDS             complement(4250..5986)
                     /locus_tag="IWO52_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0355851.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5983..6687)
                     /locus_tag="IWO52_001578"
     CDS             complement(5983..6687)
                     /locus_tag="IWO52_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0355852.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6818..7321
                     /locus_tag="IWO52_001579"
     CDS             6818..7321
                     /locus_tag="IWO52_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0355853.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7281..7619
                     /locus_tag="IWO52_001580"
     CDS             7281..7619
                     /locus_tag="IWO52_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0355854.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7640..9058
                     /gene="ffh"
                     /locus_tag="IWO52_001581"
     CDS             7640..9058
                     /gene="ffh"
                     /locus_tag="IWO52_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0355855.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9170..9748
                     /locus_tag="IWO52_001582"
     CDS             9170..9748
                     /locus_tag="IWO52_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0355856.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9752..10273
                     /locus_tag="IWO52_001583"
     CDS             9752..10273
                     /locus_tag="IWO52_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0355857.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10352..10906)
                     /locus_tag="IWO52_001584"
     CDS             complement(10352..10906)
                     /locus_tag="IWO52_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0355858.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11159..11434
                     /gene="rpsP"
                     /locus_tag="IWO52_001585"
     CDS             11159..11434
                     /gene="rpsP"
                     /locus_tag="IWO52_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0355859.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11446..11697
                     /locus_tag="IWO52_001586"
     CDS             11446..11697
                     /locus_tag="IWO52_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0355860.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11822..12613
                     /locus_tag="IWO52_001587"
     CDS             11822..12613
                     /locus_tag="IWO52_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355861.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12783..13304
                     /gene="rimM"
                     /locus_tag="IWO52_001588"
     CDS             12783..13304
                     /gene="rimM"
                     /locus_tag="IWO52_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0355862.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13294..14055
                     /gene="trmD"
                     /locus_tag="IWO52_001589"
     CDS             13294..14055
                     /gene="trmD"
                     /locus_tag="IWO52_001589"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0355863.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14191..14538
                     /gene="rplS"
                     /locus_tag="IWO52_001590"
     CDS             14191..14538
                     /gene="rplS"
                     /locus_tag="IWO52_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0355864.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(14670..15806)
                     /locus_tag="IWO52_001591"
     CDS             complement(14670..15806)
                     /locus_tag="IWO52_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0355865.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15922..16488)
                     /locus_tag="IWO52_001592"
     CDS             complement(15922..16488)
                     /locus_tag="IWO52_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355866.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(16624..17277)
                     /locus_tag="IWO52_001593"
     CDS             complement(16624..17277)
                     /locus_tag="IWO52_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355867.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(17327..17755)
                     /locus_tag="IWO52_001594"
     CDS             complement(17327..17755)
                     /locus_tag="IWO52_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0355868.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(17760..18134)
                     /locus_tag="IWO52_001595"
     CDS             complement(17760..18134)
                     /locus_tag="IWO52_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0355869.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            18438..18623
                     /locus_tag="IWO52_001596"
     CDS             18438..18623
                     /locus_tag="IWO52_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0355870.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            18636..18884
                     /locus_tag="IWO52_001597"
     CDS             18636..18884
                     /locus_tag="IWO52_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355871.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(19187..19645)
                     /locus_tag="IWO52_001598"
     CDS             complement(19187..19645)
                     /locus_tag="IWO52_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355872.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            19813..20130
                     /locus_tag="IWO52_001599"
     CDS             19813..20130
                     /locus_tag="IWO52_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355873.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            20063..20542
                     /locus_tag="IWO52_001600"
     CDS             20063..20542
                     /locus_tag="IWO52_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAQ0355874.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            20992..21423
                     /locus_tag="IWO52_001601"
     CDS             20992..21423
                     /locus_tag="IWO52_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355875.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            21420..21746
                     /locus_tag="IWO52_001602"
     CDS             21420..21746
                     /locus_tag="IWO52_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0355876.1"
                     /translation="MKELTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSL
                     IAIDSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEI
                     IAHEPE"
     gene            21865..22551
                     /locus_tag="IWO52_001603"
     CDS             21865..22551
                     /locus_tag="IWO52_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0355877.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            22526..23875
                     /locus_tag="IWO52_001604"
     CDS             22526..23875
                     /locus_tag="IWO52_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0355878.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            23866..24339
                     /locus_tag="IWO52_001605"
     CDS             23866..24339
                     /locus_tag="IWO52_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0355879.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            24341..24841
                     /locus_tag="IWO52_001606"
     CDS             24341..24841
                     /locus_tag="IWO52_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAQ0355880.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            24856..27168
                     /locus_tag="IWO52_001607"
     CDS             24856..27168
                     /locus_tag="IWO52_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0355881.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            27415..>27723
                     /locus_tag="IWO52_001608"
     CDS             27415..>27723
                     /locus_tag="IWO52_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAQ0355882.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     E"
BASE COUNT         9134 a         4910 c         5665 g         8014 t
ORIGIN      
        1 cgaaaaaaag agctacgaca tagaactgtc gtaacttttt ttggctcttc gtcaaatcgt
       61 gttggtgaag acaaatttga ataaaactta gtccgatatg cggcgcgttc tgtgcgccgc
      121 atattttttt agttttcaaa gatcagacct tgttgtaaat agatccgtgc ccgaaagtta
      181 taaaagttgc gataaccaaa cgcaattcgt ttaatcagct tgatcttgtt attcagcccc
      241 tcagtgacgc cgtttgagtg ggacacctta aaggcatttg aaatacccga ttggtagcaa
      301 tggaagatcg tgaatttctt tttaaacgac tgaggtaacg tagatgaaat tgttttaagg
      361 gtttcttgaa agccttccgt acctcgttta acgaaataat agcgtaataa ttgacaaaaa
      421 tgatacgcag ttcgcaattc ttcgttgtag cttaacagct catcaacgat ttctgtttga
      481 ctaattggtc gtttaaacag tcgatgataa tgccgtttga gtggctccca tgtcgtggaa
      541 tctttgagta agagtttcca atatcgcttg agccgacggt acttcttctg attttcagag
      601 tgtgaggttc gaaaacgatt catgatctgg acacgtaatt gattgaagct gcgattgatg
      661 tgttggacaa tatgaaagcg gtcagttacg atctccgcat ttggaaagac tgttttaagc
      721 aactgacaat agctggcatt catgtccatc actaggtact tgacctttaa acgggccttt
      781 ctaggaaagc gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta
      841 aatagctgat tggttgtccc atcaacacaa ataaaactca tggaaccaga aacagaattc
      901 attgatttga actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa
      961 tgcttcgttg cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta
     1021 gcgattagtt tacgtgattg attttcgtac agttccatga gaatcgcctg cttcagttcc
     1081 tttgttagtt gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa
     1141 tcatggcaga gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc
     1201 aacatctgag tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt
     1261 tcacaatagg aagaagagtc atccaatttt cctttaataa actgcgcttg aacgccttta
     1321 tattcttttt caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt
     1381 aattttctag tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac
     1441 taaattttac caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa
     1501 aatcgccttc gattttccac caacacaaaa tattatagaa cccttttttt ttgctatatg
     1561 acaggaatga aaggtgtttg caagcttaat gtgaggaaca ctttccatat ctgttctata
     1621 ctggatagga gaagtttgga aatatccaga aaacgaaatc aatcgaggta gccacatgaa
     1681 gaataaatgg ataaatcgaa gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt
     1741 catccgtctt tttccatcgt ataaagaagg attaacggca gatcaagtca aggaacgaat
     1801 agaaaaaggt gcagcaaata attcagtcga tcccacattc aaaacaaatc agcaaatcgt
     1861 attggaaaac atatttacgt atttcaattt gatctttctt atcttagcca ttttgctttg
     1921 tttagtagaa tcctataaga acttgacttt tttaccagtt atcatagcca atacaggaat
     1981 cgccattttt caggaaattc gatccaaaaa gatcttggac gaactaaatg ttttaaatac
     2041 agcaaaagtc aatgtcatac gagacgggat agaacaaaca atagatatag aagaccttgt
     2101 aatagatgac attgttattt tagagactgg atatcaaatt ccagcagatg cagaggtcgt
     2161 ggaagggaaa ctgcaagtta atgaggcttt attgacaggc gaagcagatg aaataaccaa
     2221 agaaataggt gatccattaa tgtccggcag tttcattgtt tccggaaaag cttacgcccg
     2281 attagacaaa gttggagcag attcgtatat ttcccagctg acagtcaaag ccaagacaat
     2341 gggagaagga gaacagtcgg agatgattgc ttctcttaat caattgatca aatggattgg
     2401 gatcatcatt attcctatcg gtatcctgct tttttctcaa agttactttt ttaatggaaa
     2461 cacgatcaaa gaaagtgtcg ttgcaatgga agcagcattg atcggtatga tacctgaagg
     2521 actgtacttg ctgacgacga ttgcattagc attaagtgcg actaaactag caaagcaaag
     2581 agttctttta cacaatatga aaagcattga gacattggca agggtaaatg tcctttgtgt
     2641 agataaaaca ggaacgatca cagaaaataa aatgtctgtt caaaaaatga tcgtctctaa
     2701 aaaacaagaa ataatcagtc agtcagatca attggaagca tgcattgctg actatgcaaa
     2761 agcgatggca ggagacaatg caacaatgga agctgttaaa gcatatttta caaaaaccac
     2821 tgatcaatcc tatcaccatg ttattccttt ttcttctgtc caaaaattca gcagtgtagc
     2881 gttggcagac aaggtgtatg ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc
     2941 ggaatatgcg gaagagtttg tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg
     3001 tgtgtacgat gggattcttg aagaaccagt attgacagaa gcagtcacac cattaggcta
     3061 tattctgatt gctaatccta tcagaaaaga agcaagagcc acatttgatt acttcaaaaa
     3121 acagcaagta gccatcaaag tgatttctgg agataatccg ctcaccgtat ccaatgtagc
     3181 tgtccaagca gggatttcac atgctaatca gtacgttgat gcatctaaat tatcagaaga
     3241 agaatatgaa gcagctgtcg aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa
     3301 gaaaatcttc gttcaattga taaagcgtaa caatacagta gctatgacag gtgatggcgt
     3361 gaatgatatt ctagccatga aagaagcaga ctgcagtata gcgatggctt ccggaaatga
     3421 agccgccatg caggcctcac aagtggtctt acttgattct gacttttcac gcatgccaga
     3481 agttgttgca gaaggacggc gcgttgtgaa caatattgag cgatcagcca gcttgttttt
     3541 agtgaagaat attttctcct ttttgctctc actgttttca gtgatctttg ctctgaccta
     3601 tcctttagaa ccatcacaaa tcacattgat tagtttgttt actatcggac ttccatcttt
     3661 cttgcttgct ctagaagaaa acaaaaagcg aatcagaggg aaattcatca tgaatgtgat
     3721 ggaaaaagct gtgccaggtg gactaacaga tatgatcgta gtcggtgcgt tggtgatctg
     3781 cggggtgact ttgaatctaa ataaaacaga tgtttctaca gcatctacca tgcttttgat
     3841 tgcagtaggg ttcctcgttc tctataaaat ctgttcaccg ctgaataaat tcagaagtca
     3901 gatcattctt ttctgtgcta gcggtaactt tttttcggtc atctttttgc ataagctgtt
     3961 ttcaatcacc ggtatctcag ctgtatcact tttgctgttg agcattctat ttttctcggc
     4021 agattcgatt tttaggcatt tgaccacatt ggtagaatat ttgtttgaac gtaaaaatga
     4081 aaaagagcaa aagccgttta gtcgcgtagc taaaaaattt ctatcgatat ttaaacgaga
     4141 aaattgacga aaagtatctc aaaaaagagt aaaaaagaac aaccactcgt gtatctttcg
     4201 aagcaggatg agtcatccaa ctttaaaacg cacgagcagt tgctcttttt tacattttcg
     4261 gaatggtaac ggtgaaagtc gaacctgttc caagatgact gtctacagta atcgtaccat
     4321 taaggagttg cacgtagtct tttacaatcg ctaaccctaa tccggtcccg cctgaatgtc
     4381 ggcttcgcgc cttgtctacg cggtaaaaac gctcaaagat ccgttcctga tcctcaatgt
     4441 cgatacctat accaaagtct tggacagaaa acgatagatc atctgttgcc tgatagcgga
     4501 taataatctc gctgtcgctt tttgaatatt ggacagcatt ttcaatcaga tttttgataa
     4561 ttggataaag gatatctgtt ttagtagtaa atggttcgtt ctttccaatc aatcgaatcg
     4621 tcaaccgttt cttttcgatg attggctgat aattttgaac gatttgctgg aaataaggtt
     4681 ctgtataaat tgtctggatc tcgtaagaat tctcgccatc ttttgacagc tggatgattt
     4741 cccgaatcag tttatctaac cggatcgcat ctttttgcat gatctcaaga aaagaagtca
     4801 gagtttgcgg gtcatctttt gcaccatcca gtaaagtctc ggtaaaacca atcagtgaag
     4861 tcacaggagt ttttaattcg tgagagacgt tgccaacgaa atccttttgt agcttttcta
     4921 aacgtcgcac tttcgttaag tcatacgcca cccctagaat ctgtccggta ccttctgaat
     4981 tagtaaagta gcgcaaactc aagtctaatg tctgccgacc ttctgtaagg gtgatctcct
     5041 gatggatcag cggatgctca ggagtcactt gatgaatcag ctgaatgagt tttggctctt
     5101 gaatgacttc tgtgtaccgc tgttcaggtt gatagcttac cacgtttaaa tgtagctgca
     5161 tctttggatt aagcagtagt aaacgagaat cagcatcatc aatcaggaat acgccaatca
     5221 tcagttcgtt cagtaacgta tataattgct cttctgtggt agtacacgcc cgatacatct
     5281 tactcatttg ttcgctcaac gcattaatcg tctgatacaa ttcttcccat tgatcgggtg
     5341 tttgcatgat tacttccgcc tgattaggat tttgcaccat tttttttagt acgggcagca
     5401 cggttttcaa tggctggttt ttttgataga tcagataata gatcattcct gcaagaacaa
     5461 ggaagaaaga cagaaagaag aaaaagaccc aacggcgaaa actctcagtt ctcggtaaaa
     5521 aaacgcttgt cggttctgcg atacgtaaaa tggcttctaa ctggccactt tttttgactg
     5581 gtaatgcgac atataaaaga tcctcattca aagtagtact tttgcgcaaa gcgctaccta
     5641 ggttcccacc attcaatacg gctttgattt cgggacggga gtttcgctgt tcattcaatg
     5701 tttcattgtt tgtgtcaaat aagatctttc cagtagcatc cattaaagta atccgttcgt
     5761 tcgactgatg gacgaattct tccaaagcag ttttgttttg cgctgcttgg aggttttcga
     5821 catctaattg ccggatcagc agttgtgctt tttttgtcag gtagctttct tgctgttcca
     5881 cgatctgctg ttgaaaataa tgggaaatca attgccagcc accaaaaaac aaactaacta
     5941 aaagaataag aaagaagaga cgttcataaa tttttttcca tttcatcgtt tgggttcctg
     6001 aaacttatag ccaaatcctc taatagtcac caagtacttt gggtgttttg gatctcgctc
     6061 gattttttct cgcaaatgac taacgtgtac atctacgatt cggctttgtc ctgcgaagtc
     6121 aaaattccaa atgcggtcta gcaatgtgtc tcggtcgatc acacggtctt ttcgtttcat
     6181 gaaataaaca agtaattcaa attctttcgg tgttagttca atcagatggt cgtctacggt
     6241 cacttgataa ttggtcaaat cagcagtgat ttgtccaatc gatagatatt caggctcagc
     6301 ttcatctttt gattgcttac gaggttcaat tcgtctgaat attgctttca tacgggctaa
     6361 tacttcgcga ggactaaatg gtttggtcaa atagtcgtca gcacctattt ctaggccaat
     6421 gatccgatcc acttggtcat ctttagccgt cagcatcaaa atcggtgttt catttttttc
     6481 ttgacgcagc cgctgagtga tctccatgcc atccatcgtt ggaagcatca catctaaaat
     6541 gatgaaatca aatgattgtt ctaatgctaa ttctaatcct tcgcctccat ctgtggcact
     6601 ggttaccttg tagccttctt tttccaaatt gaacgtcaag agcgttacaa tagaaggttc
     6661 atcatcaaca actagtattt ttttcattca aatctccttt aaataggttt cattctgttt
     6721 atattttagc atagaatcag tacaaagggc tattttacaa aagaaataaa tggtataata
     6781 ggaaggcact ttgctgaata gaaaggaaga gcagtcaatg attggaatca gtgcatgttt
     6841 aggcggtctg gcctgtcgct atgatggaca gaaaaaagaa gtaccagaat tgatggaact
     6901 agttgaaact ggagaagctg tcatggtctg tccagaagtg atcggcggat taccaattcc
     6961 tagagatccc gctgaaatca ttggtggaaa tggattcgat gtgtggaaag atcaagcaaa
     7021 agtatggacg acaagcggcg aagatgtaac ggaagcgtac aaaaatggcg cgatcaaagc
     7081 ctatcaaaaa ttgcaggaaa aacacattga tctagtcatc atgaaggaaa aaagcccttc
     7141 atgcggaacc cattcgattt atgacggaag tttttccgga ataaaaaaaa atggtgccgg
     7201 agtggctact gcttatttta ttcagcaaaa aatgactgtg ctctctgatg aagaatggct
     7261 gaaaactaga ggtgagcaga atggaaatcg aaaagaccaa tcgaatgaac gcgctctttg
     7321 aattttattc gaccttattg acagaaaaac aaatgaacta catggaatta tattatgcag
     7381 atgatttttc attaggtgag atcgcggaag aatacgaagt aagccgacaa gcagtatatg
     7441 acaatatcaa gcggacaagc aagatcttgg aggattatga aaaaaaactc catctttttt
     7501 ctaattatat tgttcgagaa caggtattgg aaaatatgac tgcatatatt acagataagt
     7561 acccggaaga taaaaaacta cttgaatatg tacagcaaat tcaagcaatc gaagaataaa
     7621 taaaaggaat gaagaaaata tggcatttga aagcttaact gaacgtctcc agcaggcgat
     7681 gggaaaaata agaaaaaaag gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga
     7741 aatccgcctt gccttactag aagccgatgt taacttgcaa gtagtcaaag attttactaa
     7801 gcgcgtacgc gaacgtgcgg taggggcaga agtgctggaa agtttatcac ctgcacaaca
     7861 aatcgtaaaa atcgttgatg aagaattgac gatcacactt ggttctgaaa cagcagagtt
     7921 aaataaatca ccaaaaatac caactgttat catgatggct ggtcttcaag gggcaggtaa
     7981 aacgaccttt actggtaaat tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct
     8041 cctaatcgca ggggacgtat atcgaccagc tgcgatcgac caattgaaag tattaggtca
     8101 acagctagac gttcctgtat ttgatatggg aactgaagtc agcccggtag agatcgtccg
     8161 tcaagggatg gagttagcaa aagaaaagaa aaacgactat gtattgatcg atacggcagg
     8221 acgtctgcat atcgatgaaa cattgatgga tgagttgaaa caaatcaaag aattgacgca
     8281 accaaatgag attttgcttg ttgtcgatgc catgactggt caagatgccg tcaacgtagc
     8341 tgatagcttc aaccagcaat taggtattac aggggtcgtc atcactaaac ttgatgggga
     8401 tactcgaggc ggggcagcac tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat
     8461 cggttctggt gaaaaattga ctgacttaga agtattccac ccagatcgta tggctagccg
     8521 tatccttggt atgggggata tgctgacatt gattgaaaaa gcgcagcaag actacgacga
     8581 gaaaaaagcg gaagaacttg cacagaaaat gcgggaaaat tcattcgatt tcaatgactt
     8641 tattgaacag cttgatcaag tgatgggtat gggcccactg gaagatctaa tcaaaatgat
     8701 cccaggaatg aatcaagtcc ctggaatcga aaacgtcaag gtagatccaa aagatgttga
     8761 acgcaaaaaa gcgatggttt attctatgac gccagcagaa agagcaaatc ctgatctgtt
     8821 gaacccaagc cgtcgtcgcc gaattgctgc cggttctgga aacagtgttg ttgaagtgaa
     8881 tcgaatgatc aaacaattca aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga
     8941 tatgaacatc ccaggtatgg accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa
     9001 aatggcgatg aatcgaatga tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga
     9061 tgaaaaaaac agcttcggta cagactgaaa atgtctactg ccggagcttt tctatcagca
     9121 atttttattc tttactaata caattactct aaacgaagag gtggcacaga tgagatgtcc
     9181 gtggagtgaa caaacagaaa gtatgcggca atatcatgat actgtctggg gtgttcctga
     9241 atatgatgac caaaagctgt tccgaaaatt gatgcttgat attaatcaag ccggattgag
     9301 ctggcagacg attttaaata aaagtgaagc attcgatgaa gcatatgatc gattcgatat
     9361 caaaaaagtc gcaaattatg gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat
     9421 tattcgtaac cgaagaaaaa tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat
     9481 tcaagaagaa atcggcagtt tttcagagta tctttggtcg ttttctaatg gaaaaatcgt
     9541 tgattcttct tatcattcac aagaagaagt cccaacaaca agtcctcttt cagatgaatt
     9601 agcaaaagat ctaaagaaaa gaggatttaa atttatcgga agcacaacaa tctatgcttt
     9661 tttagaagct gtcgggatca tcaatgatca ccttgacagt tgtttccgca aagaagaggt
     9721 cgtacgtatc ggcagaaagg gcgaataaag catgcgaaaa atcatatttt atggtgctat
     9781 aagtttagat ggctatttgg caacgaaaac ggatgatctt caatggcttt ttgacactcc
     9841 aacaggtaag caaactactt atgaagcttt ttatgaaaca atcgatacga ccatcatggg
     9901 cagaaaaact tatcaggaag caaaaaaata tttggagacc gaagcaattt atccagagaa
     9961 aaagaatttc gttttttcca cccacgcaca attacaattg ccagatgctg aggtcgtaaa
    10021 cgaagatcct gttgcttttc taaaaaatct acaaatgaca gaaggaaaag atatctgggt
    10081 tgtgggtggc ggtcgattga tcaaaccgct attggaacat caaatgattg atgaatggta
    10141 tatccagatt acccctgtat tattagggga cgggattcct ttattccaaa aaggaacata
    10201 tgaagcgcgt tttgaaatgc tagacacgac acgattcggt gaatttatcg aattgcatta
    10261 tgtacgaaag tagaagtaaa taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac
    10321 atagttgcct tttttattac cgttatgctt tttactctgt taatttgtta gaaaaatcta
    10381 gatacgcatc taaaatcgtt tggaagaaac ttaatgttcc ttcaacgata ttaccatttt
    10441 catcaattag attcgcaacg tttccgatat atgcttccgg ttgctgcaag gttggcacat
    10501 tcaaaaagac aagtgattgg cgtagatggt ggtttgcgcc aaagccgccg atcccacctg
    10561 gtgatgcagt aacgactaaa cctggtttat gatcccaaac actgtgtcca taaggtcttg
    10621 agcctacatc taaagcattt ttcaatactg ctggcacaga acggttgtat tcaggagtaa
    10681 cgaaaacgac accgtctagt cccctcactt cctcacgaaa acgagtccat tcagcaggag
    10741 cctgttctgg agtttcgaga tcctcattgt aaaatggcag atcatcgatt tttacaaata
    10801 cactttcgta cccttcagga agcaatttcg caaaagcttc tgctactttt ttgttgtaag
    10861 aatcttttct taaactacca ataaaaaatc caattttttt tgtcatacga tttttcctcc
    10921 ttcaaactat ctataataat cttacaaaag ataagtaact ggagcaaata aaaacactta
    10981 ctgacaaaag ctacatagaa gatagattac ttatttttaa caaaaaagaa gtctaaaatc
    11041 ttctattttc taaaaaaaat cgaatttaat caagctgtaa agaaaaaaag ctttacaggg
    11101 taaaaaaaaa ctgttacact acatcttgtg aataaaacaa tggaggtgga aataaataat
    11161 ggcagttaaa atccgtttaa aacgtatggg ttctaaaaag agtccgtttt accgtatcgt
    11221 cgtagctgat tcacgttctc ctcgtgatgg acgtttcatc gaaactgtag gtacttacaa
    11281 tcctttgaaa gaccctgcag aagtagtttt aaaagaagac ttagttcttg actggttatc
    11341 aaaaggtgca caaccttctg acactgtacg taacatcctt tcaaaagaag gcgttatgca
    11401 aaaacaccac gaagctaaat tctcaaagaa ataaggtgac cggatatgaa agatttaagc
    11461 gacttagtct taacaatcgt tcgtccgcta gtcacttatc ctgatcaagt caagttagac
    11521 gtcgtagagt cgaaagactt ttacgagtac aatctaacag tggctcctga agatattggc
    11581 cgtatcattg gaaaacaagg gcgtgttgct aaagcaatcc gcacaatcgt ttacggtgtt
    11641 cggatcaatg caccgaaaaa agtgcgtttg aatatcatcg atgataagga aaagtaaata
    11701 aacgtgctgt actcgaattt tcgagtgcag cttttttgtt ttttttatta attgaagaaa
    11761 tgattatttg gcaaaacaag caagaaagcg tatccttata ggaggaggga gtgaacagcc
    11821 aatgagaaag aaaatgttat ttattccatt tgctctattg ttaagcgctt gtagttcggt
    11881 tactcaagaa tcagacaata cacatctatc aagcagtata tctgaaactg ttaccagtcc
    11941 agaaaaaagt agtaccaaaa cggcttccac cactgaacaa acaatggcta cctcaaataa
    12001 tgagaaaaag actgctttag accagttgaa agaacaacag ccaaatgttc ccatgcctct
    12061 agatgtacca gtctcttcag gctatctcaa tatcgcagcg acacatacaa agcaaggtta
    12121 ttctatttta tattacagaa cagatcgtcc cctcgggttg aatgctgacg aattgaatca
    12181 agaaacgcct attgcaacct atctatatca atatgggttt gcttctagtc aagaaacaat
    12241 ccaagttttg cagccatttg agatcgacac aaatggtcag caagtggact taggttctcg
    12301 aatcacaggg tatcagcaag gtgcagcagg ttcaagtttt cttgaatggc aagaaggaaa
    12361 ctggcgtatt cgaatccgtg ggaataatat agaaggccaa gatcccttac tattagctaa
    12421 agaaatcgta gcttatttgg aagaaaacag tctaccagca cctgaacaat ttggtaaaat
    12481 tactgttgat atgggtgata cgacgaatcg agccgtagaa gtaagctggc aagaaccgaa
    12541 aaatgcctat acgatcactc atcaagatcc tatgtctgcg ttgaagatgg cagtttctat
    12601 gaagaggttg tgaaatcagc gcgctgatct gagcccatca tttaactcca agtcacagga
    12661 agtaatcaaa aatagcacgc cgcatttttg cataattttg gcttaatacc ttaaaagaaa
    12721 actagaattg cctcttcaca catgctaaaa taaaacagac aaaaaagaaa tggggatata
    12781 agttgactga atacttgaat gtaggaaaaa tcgtaaacac acaaggtatc aaaggcgaag
    12841 tccgtgttat ctcaacaacg gatttcccag aagaacgtta caaaaaagga acggtgctaa
    12901 cactattcca agacggaaaa gcacctgtgg aattaacagt caaaagtcat cgcaaacata
    12961 aaaattttga cttattgagt tttgaaggac atccttctat caatgatgtg gaaaaatatc
    13021 gggatggcat attaaaagtg tcaaaagaca actctgtaga tctagctgaa aatgaattct
    13081 attaccatga gatcatcggt ctgcatgtat tagaagatgg aactgagtta ggtaaagtaa
    13141 aagaaatctt gtcaccaggt gccaatgatg tttgggtcgt tcaacgtccg aaaaaagctg
    13201 atatattgct tccatatatc gcttctgtcg tcaaagaagt cgatttagaa gctggagtgg
    13261 tccgtgtaga aattccagaa ggactgattg acgatgaaga ttgatgtatt gactttattt
    13321 ccaagaatgt ttgaaggtcc tatgggggaa tccatcatcg gtaaggctgt agataaagga
    13381 ttattagata tcaatatctc taacttccgc gattattcag ataacaaaca tcagacagta
    13441 gatgattatc catacggcgg tggagcaggt atgctgctca aagtccagcc catctatgat
    13501 aacatccaag ccattgaaaa agctgcaccg gatgtcaaaa aaagagtaat tcttcttgac
    13561 ccagctggta aacgattcga tcagaaaatg gcagaagaat tttcacaaga agaacactta
    13621 gtattcattt gtggtcatta tgagggatat gatgaacgta tccgcacatt agtgacagat
    13681 gaagtttcct taggagatta tgtccttact gggggagaat taggtgcgat ggtcatgatc
    13741 gatgcaacag ttcggctttt gccagatgtg ttagggaatc agacttcagc tcaaacggat
    13801 tcttattcaa ccggtttatt ggaacatccc caatatactc gcccggcaga atataaaggg
    13861 atgaaagtac cagaagtatt gacaaatgga aatcataaat tgattgaaga atggcaatta
    13921 aaagaatcac taagaaggac ctatcaacgt cggcctgatc ttttggaaac acttgaatgg
    13981 acacctcaaa tgacgaaatt tttagaagaa atcaagaaag aagaacaaga aaaaacagca
    14041 gatgaagcca agtgactttt tctaaaatct tctttacagt aggttgataa tatgttagta
    14101 ttttatggtg agtgcaaaag cacttaacta ttacgatatt ccgctgtgag aaaagctcat
    14161 aagaatattt ggaataagga gaagaaaacc atgaatccat taatcgaaga attaacaaaa
    14221 gaacaattac gttctgacat cccagcgttc cgccctggtg acactgtgcg tgttcacgca
    14281 aaagttgttg aaggtactcg tgaacgtatc cagttatttg aaggtgttgt tatcaaacgc
    14341 cgcggtgctg gaatcagcga aacttataca gtacgtaaag tttctaacgg agtgggcgtt
    14401 gaacgtacat tcccattgca tacaccacgt gttgcaaaaa tcgaagtagt tcgctacggt
    14461 aaagtacgtc gtgcaaaatt gtactaccta cgtgcattac acggaaaagc tgctcgtatc
    14521 aaagaaatcc gtcgttaatc atcgttatca gacagagaaa gaacccttgg tattcaaggg
    14581 ttcttttttt attatctgtt tttttatatt aacgatttct atttggtaca tttttgatta
    14641 ataaaaaggg gcaaaaaagg ggcaacaagt tacagttttt ctatatcttc tttcaatttt
    14701 tcttttactc cttttgtcac atgcaaataa attttttcag tgatatcact attttcgtga
    14761 ccaacgcgat cctgaatggc atacagagga gttcctagtt ccgctaattt tgaaatatgg
    14821 gtatgtctaa aaatatgtga actaagtttt ttatcaatct tcatgtcagc tttatgactt
    14881 ctcaaatagg tgttaattgc tgttagttgg aaaggagttc ctttcgttgt ttgaaataga
    14941 aattgaccat ttggatttaa ttctagaagc tcattgtaaa tagctatcgc tttttttggt
    15001 aaatcaattt ctcgcattcc ggcagctgtt tttgtagaat cagatttttt catatcagct
    15061 attgaacgtt ctctatacat catcgtccca tttataacta ctgatgcatt attattggta
    15121 atgtgtacat catctttact taatgcgatt gcttcaccag acctcatccc agtcaaatat
    15181 agccactgga aaaggagaga atatctttta ttgtgtgaag ttgtgaaatc tactagtcta
    15241 ttatattcat catcttctaa aaatttatct ttaattttta tcgtttttga ctctctttta
    15301 taatcaataa ctacttcatc gataggattt ttttctacat aacctttttt catagcatat
    15361 gaaaaaagaa gattcaattt ggatttaatt acactgacgt acttatttga caagtcatct
    15421 ttatatatca tgtcttcaaa aatattattc aaatctattg tattaatacc agaaacgatg
    15481 taagtctcag gtatcttctt ctttatagtg tttaatatat tattcgtagg ataatatgta
    15541 gattccttaa cttgtctttt gtatataact aaccattctt caacaagctc atgaaatgtt
    15601 agatcaggct tatgaagtgt tttttcgttt aattttatat caattttttt atttaactct
    15661 agcaatgcca ctttctgagt ttctcgagat ttatttttat aagtaatgct cacttttttt
    15721 ctttttctag tttttggatc aatatatcgc tcattatatt tatatacttt ttttccgttt
    15781 ttatcagttt tagtttcgat ccacattgtt ttcatctcct atttgttgct ataataggaa
    15841 tagataagta agccgattat agcaggttta tttttcatca cgtccacaaa ctttggcgag
    15901 ggaggggcgt gtttttgtta gttattgtgg taggttatct aaagcgtatt gtgcttgctc
    15961 atttgtaaaa ccttcaaata taagttgatc gtacaattgc gaatctgaca ttgatgccca
    16021 atcatcgtaa ctttccgctt tagccaaagc ttgttcgttg taatcgacat caacattagc
    16081 cagcgcataa tcgattgcat cacttggata ttcttcaaac tctagttgtt cacgcaaacc
    16141 ttgttcagac ataccagccc aacctagata gctttcagct ttgctcaatg catttctgta
    16201 ttcacgagga acactttctt cttgagtaac ttcttctata gtagtttcag tagtttcagt
    16261 ttcttcttca acgactgtag attctgctac cgattctgca gaagacactt ctgtcacgct
    16321 ttctttagtt gtttcagcga cggtattttt agcatcatca ctgcctcctt gcgaacctat
    16381 agcaataaac accacaacaa ccgctaacaa ccaaaaccat acccgtttat aaaacggttt
    16441 cttaacttta tacattttcc catcttgacc cataactttt tttgccattt aaatatccct
    16501 cgcttcttgt tataatatat ttgtgatctc agaaatgagg tatgagtccg tgttgcagca
    16561 cgggcttttt tctttataac ttttttagag attataggca aaatagtagg gcataaaaat
    16621 gtattattga attccgtatt tagaaaatcc taattgaact tcaccggaag tcttttgctg
    16681 tgttgtacgc aatgcttctt cttcagacat tccattctgt actttccatg caacaggcga
    16741 catcccgtat ttgttaacaa aatcagtaag tgataaagtg tcagcgtctt gctgagcgct
    16801 tgtttgttgg tcttctggat tctgttgaga tgctgcttgt tgttctttct gatcttgact
    16861 gataatattg ccagcatcat ctgtagtcaa tccattttca taaagggcca cgccgaaagc
    16921 ttcccactct ttgttggacc aatttgcacg atcagctgga gttgactgta aagtgcgttg
    16981 tttcatctgt tcatatgttt cttcttgagg tgcggtttgg attgtactct gactggagct
    17041 tataactgtt gggctaggtt ccgccgtagc ttggttggag cttgtaactg ttgaactaga
    17101 atctgtttta gatgtagatt tgctagtaga tgaactggtt tcagttgttt cttttgtttt
    17161 acttattttt gtttcttggt tagaagtggt atctgttgat tcagcttttt tattatttga
    17221 acaagctgaa agtagcagag cagtacttaa caacaacata acgctaactt ttttcatttt
    17281 ataattcctc tttctcgttg taatatgtgt gctaacacgg gcttttttat ataagaaaac
    17341 gataagcgct ttctggaagc ccgtaaagat tctttaattc ctcgattttt ttaggatatt
    17401 gatcattatc ttctttataa agagaaacaa tgagattagc agcaaagcaa ttagcttcgc
    17461 tttcagattt gcttctagat gttcttgttg atacataata gctggataag ccacgatgaa
    17521 aaatagcgtg acctaattcg tgagcgcaaa tgtagaatct ttcctcagag tctcgcagtt
    17581 catcatttaa aaagattatc gcacgatctc taatttcttg aaactgcccc ttgggattct
    17641 cgataaaagg aacgtattga attttaatgc ccatcttttc acaaatataa aaaggattag
    17701 cggactggta tttccgcttc aactcctcga ctaaattaat cgtatccatc tccataagct
    17761 cacatctttt tgcctttttc tttgtcttct ttcacaatat cccagaaagt cgctatcagg
    17821 atatctttta cgcgctgtat ttgttcgggt gtcaatgttt ccccaccata agacatatta
    17881 acatttgagt ctagtagttt atcaagttca accacttcct cttttgtagc ccacttggga
    17941 acattattat ttcccaataa ataatcagtt gtgacaccaa aataatcagc aactttcttt
    18001 aagttctcag attttggcga ggctttatcc catcttctta tttgtccatt agagatgccc
    18061 acctgtcttt ctacttctgc tatagtcaca tgcttttcgt ctgctaattc tttaatctta
    18121 gtaactaaac tcattattat caacctttca aagctgaaaa gaaaataaat agcttaaaag
    18181 ttatattttt ggttgacaat tagcttttaa gctagtatat ttaattcgta agctaaattg
    18241 ttagctaaat aagagcaaca aaaaactcta ctaatttaaa acattctctc ggtcgccaaa
    18301 cttagaaatg ttattttaga ggctttttat aagtcttatt taactatgta ttcataatag
    18361 cttaaaagct aataggtgtc aacgatttag ctaatttttt agcttacaaa ttatttgttt
    18421 agaaaggagc tatttttatg tctgagaatt tagacttaaa aattcgagcg gagatgagaa
    18481 aaagaagaat gactttcaaa gaactagctg cgcttatcgg tatttcagga gcttatttat
    18541 cagatattct aaacggcaat cgtgatggaa agaaagcaca acagcatatc gaaacagtga
    18601 aaaaaatatt ggacatccga tagggggggt aggcgatgac aaaactaaaa aaacaagatt
    18661 ttgtaaaaaa atataattat tctccatcta cttatcaacg tcgaatgtcg gaactaaaaa
    18721 atacagcaat tttctcagcg gcgtatgaac gggtcacagg acaagaagtt tggatcaata
    18781 cagaattata cgataaattt ttgtctttca aatcctataa caggttacgc acaagaaagg
    18841 taacacctaa agaatttatc gagaagcatt tagttgattt ataaaaaaca gaacattttg
    18901 agaggtgaag gttaatgggt aaattcaaca gagcattagt attcagcgca ccgctaatcg
    18961 tctatgcttt agggctttgg ggaagtagac aagcgttgat aggaacgatt gtgtacatgg
    19021 tttggatttt tatagggctg gatgaagctg agtacaaaac aaaaaagcca gtcgggaggg
    19081 actgactaat gagtaaggac gatattgtat ttctgttaat tatgttttgt gggattggat
    19141 caataataat taatttgtat ttgattatta aagaaaaatt tttcaattaa aatgagtctt
    19201 ttagctttaa cttaaatttt ttttgataat tagctgttct tatgaccatt acaagttttt
    19261 caccaggtaa tatatctttc atatctaaaa cttctgaaaa agcgactgag tagccaggag
    19321 agattgcttg tgccaacggt ataggagata aaaggctact tttatgaatg cttcccatat
    19381 caaaaccatt ggcgtgataa tgtgaacaat taattttctt accgttgtaa agatacgtac
    19441 attcgataat tgagtttggc atttttgatg ggtttgcaat tacgccagta agaatcattc
    19501 gattacttct atcatgctta actaaaaaat aggagagcgt aatttttctt ttatctttaa
    19561 ataagttata gagggataaa aaaaatcctg aaatagaaat gattaaagta atagcgactt
    19621 ggtaatcgct caaaattttt atcaagtaat tcaccacctt tttactcaaa ttatatcaaa
    19681 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaaaaaa gacacgaacg
    19741 ccggcaagca tgatcgtgtc agggaaataa actatctttc tatattttac cacagaaagg
    19801 aatgaaccgt aaatgctaat tgcaacggat acactagacc gcatcttttt aaaagacgaa
    19861 tacaaactgc gcaatataga tgcgtcagga attttagtat ttgatcttta tgacaatgga
    19921 aaaattggta tctatcaagc aagtgatatt gaagaaacaa accttgcttt cgaacaaatt
    19981 gatgattctg tggaattgga tttagatgag gcaatcctag cttttgaaca aattgcaaaa
    20041 ttattaaagg aggcacaaaa gaatggcaac tctttaccaa ctcagcgagt catatatcaa
    20101 agtcctggaa ctggcagaac aattggatga agaaattctt cgcgatactc tcgattcgat
    20161 taatgaagcg atcgagtata aggcagaaaa cttagcaaaa atagttaaag aagtagaagg
    20221 gaaagctgag ttaatcgata gtgaaattaa acgtctgcag gaacgtaaga catcgctttt
    20281 gaacaatgct aagagtatca agcactattt acaagaggaa atggaaaaga ctggtaaaac
    20341 gaagattaaa ggtgaattat tcaacattgg aattcaaaat aatccagtat cggtcaatgt
    20401 aatcaatgag aacttaattc caaaaggatt ctttacccct gtgcctccca aattggataa
    20461 aaagcaattg aaggaggaac tgaagcacgg agatattcct ggtgctgaac tcgtccaaac
    20521 aaaaggtttg aggataagat gatgaaggag gagaaataat ggaacacgaa aacgtaaaag
    20581 acgctttgaa agctgctatc gaaatcgcgg aagctaaagg aataaaggtt gacggcaaac
    20641 cagcaactgt ccatgacatt caaaatttga cgaaagaaca tctttatttt atcgccgact
    20701 tgcttggttt gtcggaattg tatttagaca aataacccca cacggaagga gtggctaggg
    20761 tggttgtatt acaagtaata gtctgcgtaa cattcatttg gtcgtgcata ccggtttgta
    20821 tatatatcat gaagaagctt cgccctgagt cttacaaatt atacgcaatt tacagcataa
    20881 cagtagctct tgcatttaca ttgtttgcaa tttctaggct tccgttttaa tcgtgggaat
    20941 gaatttttaa tgaactaaat tatctataaa gcaaaaaaag gagaaacaac attgaaaaac
    21001 aaactagcaa aaacaacagc aatcatcgga ctagcactag gtagcggcgt tatcggctac
    21061 gcagcaagca atgcgtttca agatttggac acgatcaaag caaatttcaa cacagtccta
    21121 caatacggtc aaacgaaatc gcaacgtgtg tcagaacttg aatcacagct atccaacaac
    21181 actcgcacac aggagcagct gaaagccgaa attgagcaaa tcaaatcgga caagcagaag
    21241 gaaattgaag ccaagcaacg agaagtcgaa caaaagcaac aggagatcgc tacaaagcaa
    21301 caggaagccg atagcttgcg ccaacaacta tccacagtgc aaaacgacaa ggagcagtta
    21361 gaacagcgcg tgagcgagtt acggcagtat acggatcaaa aggtagggga gttggggaaa
    21421 tgaaagagtt gactatttat gacccaaata tttcatgggc taaacacaca attaaagttt
    21481 cttttatgat ttggggttac aaaggatatg ttacttacaa agtgggcggt aacactaagg
    21541 gtttatcatt aatagctatt gattcagatg atctatatga tgctaatttc gaggataacc
    21601 cagttaattt ccgtgacctt gatgaagatt ggttctctat ggaacttact aacgataaag
    21661 gcgacagtac acttgtagaa gatgaattcg acagactagg tgattatatt gtcggtgttg
    21721 aaattattgc gcacgaacca gagtaggaga gtgaatacaa aatgattaaa agaatcacta
    21781 tatcagttga agaagatagt ggatcaacaa tgcagcatac cgacaatcga aaaacttttt
    21841 taaccaatag aaaggagaat tttgatgcag attaagaagg cgacggatat cgaacaaacc
    21901 aagggaacat atttgatcta cggtgctcca ggaaaaggta aaacttcaac ggctaaatat
    21961 tttccaggga aaacactgat ccttgatatt gatcggactt caaaagtcct tagaggtgag
    22021 aaaaatgtcg atatcgtata tatcgacaac gaagatacct ggaacgactg gggcaacacg
    22081 ttggctgatt taactaccaa ttatgtaggt gtttatgaca acgtggttgt cgataatgtc
    22141 agcgaattgg aacggtgtat tctgtccagt ttaggcgcag aaggaaaaaa caacggcgtt
    22201 ccttcgcaag gcgattatca atatatgcaa tttagaatgg tgaacagcct acgctatatg
    22261 aaaaatttag acagcaacct tatctggacc gcatgggaag aaatcgattt gtggacggat
    22321 tcggatggat cttcttacaa catcgctttg ccgcagatca atcgaaagat tcgaaacaat
    22381 attttgggat tatgtgacgt tgttggtcga ttgatggtaa aggaagacga tgaacgcgga
    22441 tttattttac aagccacaga ttcaacctat gccaaaaatc aattggataa tagatctggc
    22501 tgtaagcaaa gtgagctgat tttagatgga attgtacgac taccagaatg atttagtcga
    22561 tcgtgcaaga caagcctatg ttgatggtta caaggcacca tgtatcgtat caccgtgcgg
    22621 ttctggaaaa tcagtcatgg ttgcagagat cgctaaacga accacacgaa aaggcaacag
    22681 agtgttgttc ttggtccatc gtcgtgaatt gattgatcag atcaaagata cgtttcaaaa
    22741 gatgggcgta gataatcagc ttgtaaattt cggcatggtt caaacagtcg tacgacatct
    22801 taaaaccatc aagaaaccac aactgatcat cactgacgaa aaccatcacg ggttagcagc
    22861 gtcctacaga aaaatctatg agtattattc ggacgttccg cgattaggtt ttaccgcgac
    22921 acccatccgg ttaaacggta gtgggttggg ggatgtgaac gacttgctaa ttgaaggagt
    22981 atcggctaaa tggttgattg agaatcatag attagctcct tatgaatatt atgcacctaa
    23041 attgatcgat acagccgaat taaagaaagc ttcgactgga gattttacaa agaaatcaat
    23101 ggataaggca gtgaagaata ctatttatgg cgacgtgttg aaacattata aaacgttagc
    23161 agaaggtgaa caggcaatag catactgtca cagtatcgat gcaagtaaac acactgctga
    23221 aatattcaac gatgctggtt ataaagcaac acatatcgat gcaaaaactc caaaagatga
    23281 acgtgcagat attatcgaag cgttcagaaa tcatgaaatt aaaatcctat gcaatgttga
    23341 tttgatcgga gaagggtttg atgttcctga ttgttcgact gtaattatgt tgagacctac
    23401 tcaatcgtta tcattgtaca ttcagcaatc gatgaggggg atgcgttacc gaccagataa
    23461 aacgtcaatc atcattgacc atgtgggtaa tgtgaaccgt tttggattgc ctgatatgga
    23521 tcgtgaatgg agtttagata caaaaaagaa aacaaaaact gatagtgatt tatctatcgt
    23581 tcaatgcgcg ttttgttttg gtgcttacga acggccaaaa ggcgataata tttgtccgta
    23641 ttgcggagag ttgcagccga ttgaagaacg aaagtcagag ttagaaatcg atgaatcggc
    23701 tgaattaatg aaagtcggag aaacgaaaat tactttgaat tttgagaaca ataaatatta
    23761 caacatgacg gaagatgaag cagaaagtat agaggactta tatgccatag caagagctaa
    23821 aggatttaaa cctggttggg cttatatggc tgctaagagg aaggggtggt tgtaggaatg
    23881 ggcggtattt ttggtaaaaa agcagaagat gttcaaggtt caataataaa tggcgtaaca
    23941 ataataggtg atactggaaa aagagatagt tcaggcaatc aaattgtaat cgctagaaat
    24001 aaaaataacg aagtttttga atcttctcta tctaatataa aaagaggtgt tgttaacggg
    24061 acaggcgaag agagtggcta cgttaattta aaaaaagcta atgaaaaata ccatgttgaa
    24121 ggaacttacg ttaaggcttt gtctgtcggt ggaacaaaag gcgttaattg ggataaacga
    24181 agaaataaat ggaaagcacg attatatttc aaaggagaat atgtcttaca aaaggatttc
    24241 aacaccaaag aagaagcaat ccaagcacga cttgcagcag aagaaaaata tttcaaacct
    24301 atcttagaaa aatatgaaaa taaaaaggag aatgattaac atgacaggat ttaacttaga
    24361 cttttcaaaa gcgcaacaag ggaacgaaat caaagacggt acgtacgaag tggtcgtaaa
    24421 caaagctgta gaaaatgcaa ctaaatcagg cgcagaattt atcgatatcg atttgatcgt
    24481 tcgtaacgat gtggatcaac cgttccaaaa taaacacatt ttcgcaaaaa tctggaaagc
    24541 caaagctacc ggcaaatata acgaaggtat gatcatggca attgcacaag cgctacagtt
    24601 ggaagacgga aaatcatata acgggtttga tgaattgctg gcggactttg ttttgaaaac
    24661 cgcctcagta cgcgtgaaaa ctgaagagtc aaatggatat aaaaacgtca atgtgaagtc
    24721 ttgggataaa acgaacacac ggggcgtaat gaatcaccaa ttcaaaaatg gtgatgaacc
    24781 atccttcgga ccggaacgtt caagagcgac aacggttaga aatgacaatc ttccatttta
    24841 attgaggtgt aatcaatgta tgaaaaaatt ccaaaagagc ttaaaaatct caaacaatgg
    24901 tgtgtttata agctagtttg ggatgaaaag cggcagaagc acacgaagat cccttataac
    24961 gccaacaacg ggcataaagc caaatccaat gacgagagta cctggtctga ttttcagacc
    25021 gctctcgctg ctattgatca ctacggaatg agtggactgg ggttcttttt caaaccacct
    25081 tatttcggca ttgatatcga taatgctgaa ggtgaagttg agcgatttaa atcaggtgat
    25141 atagaagaaa atattatcta tgaatttatc gaaagcatga gatcctatgc tgagtattct
    25201 caatcaggta ctggcatcca cattattgct cgcggagaat tgcccggcgg aagacgcagg
    25261 aaaggtgacg tagaaatgta ccagaacggc cgattcttcg ttatgactgg aaatgccgca
    25321 tcaaaatatt tagaaataac tgagccgaat ccaaaggata tcaaacgcct atatgatcgc
    25381 tacgttggag atgaaaaaat catccaattc aaagaagaaa atccgctgat gaataccgtt
    25441 gatttaccgg ttgaagaaat tatcaaacgt gctgaatcgt cttcccaagg ggcaagattc
    25501 aaagtcttta tgaacggcgg ttgggaagct ttttattcct cgcaatccga agcggatatg
    25561 gcgttcgcca acgacttggc tttttggacc ggtagagact ttgaaaaaat ggatgagatt
    25621 ttcagagcgt ctgcaatgat ccgtagcaaa tatgaccaaa aaaggggatc ggcaacttac
    25681 ggagaatctc tactaaataa ggcaattgct gatacaaatt ccgtctacaa ccccaaacgg
    25741 aaatctgatt ttaagatttt tatcaaagac caggaacagc caaaagagga aaaatactat
    25801 tcgtacgacg acaccggcaa cgcggatcgg tttactgata tctacggaac gttagtcaaa
    25861 tattcgtaca ttgataaatc ttggtattac tacgatggga aagtttggct acaggacaat
    25921 accggcgaag ttcggaaaat gatcgatacg acagtagaca tcatgggaaa agaaccgtta
    25981 acgatccctg aaggcgcaga cgatgaaacc aaagaagctt tgatgaaagc gaaagaaaaa
    26041 cacgtcaagc gttctcgcag caatgccgga aaaaacgcta tgatggacga attgaaacat
    26101 cgactatccg tattgccgga agagttcgac aaagataaaa cgctgttcaa cacacaaagc
    26161 ggctatctgt ctttgcatga cggtcttcta cacgagcatg agatagacaa aatgtttaca
    26221 cgtgtttcaa atgtcgagta cacagaatcg gtcgattgtc ctatgtggga agaatttatt
    26281 aatcagattt ttgacaatga tcaagaatta atcagatata tccagaaatg tgtcggatat
    26341 tccctgaccg gatctaccag agagcaatgt atgttcatcc tctacggtca tgggtcaaat
    26401 ggaaagtcgg tatttttgga aattatttct gaattgatgg gaaactatgc gatgactatg
    26461 caggcacaaa cgatcatggt caagcaaagc caatcatcag caaacagtga tattgcacga
    26521 ttgaaaggtg cgcggttggt cacttcgtca gaaccgaatg aaggcgtccg actggacgaa
    26581 ggtctggtca aacaattgac tggtggtgat aaagtcacgg ctcggcacct ttatggaaaa
    26641 gaattcgagt tcgaacctga attcaagttg tggctggcaa cgaaccacaa gcccatcatc
    26701 cgtgggaccg atgacggcat ctggcgacgg ttaaatttaa ttccattcac tgtccaaatc
    26761 ccggatcaca aaaaggataa aaatctgaaa tttaaattgc agacggagtt gcaagggatc
    26821 cttaaatggg caatcgatgg ttgcctgttg tggcagagag agggcttgga gaaaccaaag
    26881 tcggttgttg cagcgagtca ggattatcga aacgaaatgg atcaaatagg tacatttatc
    26941 gaaacgtgtt gcgaaacagg tccaggacta aaaatatcag gcggagaatt atacaaagtt
    27001 tatcgtgaat gggcatcaga taatggcgag catactttca ctaacacgaa gtttggcaga
    27061 gagatttcaa agaaatatag caaagaaaaa gctggtggat ttatggttta taaaggaatc
    27121 acattaaaac caagaaaata cgataacgtt agagaacttt ttaaataaat ctggagggtt
    27181 ctggagggtt gagaacgaaa ccctccaacc cttagaaacg ttgatatatc agtatttata
    27241 ttatattttt ctttctttct ggagggtttt aaaaaaataa taaagtatta aagtaaaaaa
    27301 taaaaagtaa attatataaa aaagaatagg tttttgggtc tgaccctcca aactctccac
    27361 caaaatcttc ctaaatgctt tgataccaac gattaaacag tctggagggt tacattgaaa
    27421 tcagaacatc aaatacaaga cgagatcaga attgccttat caaaacatgg ttgcacgatt
    27481 tttcgatcca atgcgggaca agtgacaacg atcgaagggc ggcaatttta cgggatgcca
    27541 aaagggttcc cagatctatg tggacatcgt aattctgatg ggaaagcaat ttatatagaa
    27601 gtaaaaaatg aacgcggtaa gttgcgtgaa gatcaaaaaa ggtttgctga atttttaaac
    27661 ggtcaacctg tactatacgg tgttgcacga tcagtggaag aagcattgaa aattgtggag
    27721 gaa
//
LOCUS       DACEDO010000037        26646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEDO010000037 DACEDO010000000
VERSION     DACEDO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.037"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IWO52_001609"
     CDS             complement(218..1015)
                     /locus_tag="IWO52_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0355883.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IWO52_001610"
     CDS             complement(1012..2196)
                     /locus_tag="IWO52_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0355884.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IWO52_001611"
     CDS             complement(2221..3468)
                     /locus_tag="IWO52_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0355885.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IWO52_001612"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IWO52_001612"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0355886.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IWO52_001613"
     CDS             5448..5762
                     /locus_tag="IWO52_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0355887.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IWO52_001614"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IWO52_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0355888.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IWO52_001615"
     CDS             6447..7091
                     /locus_tag="IWO52_001615"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0355889.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IWO52_001616"
     CDS             7106..7435
                     /locus_tag="IWO52_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355890.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IWO52_001617"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IWO52_001617"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0355891.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IWO52_001618"
     CDS             8423..9247
                     /locus_tag="IWO52_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0355892.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IWO52_001619"
     CDS             9240..9587
                     /locus_tag="IWO52_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0355893.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IWO52_001620"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IWO52_001620"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0355894.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IWO52_001621"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IWO52_001621"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0355895.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWO52_001622"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWO52_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0355896.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWO52_001623"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWO52_001623"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0355897.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IWO52_001624"
     CDS             15244..16011
                     /locus_tag="IWO52_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0355898.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IWO52_001625"
     CDS             16001..18043
                     /locus_tag="IWO52_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355899.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IWO52_001626"
     CDS             18324..18473
                     /locus_tag="IWO52_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0355900.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IWO52_001627"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IWO52_001627"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0355901.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IWO52_001628"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IWO52_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0355902.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IWO52_001629"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IWO52_001629"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0355903.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IWO52_001630"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IWO52_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0355904.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IWO52_001631"
     CDS             22890..23972
                     /locus_tag="IWO52_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0355905.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IWO52_001632"
     CDS             23972..24775
                     /locus_tag="IWO52_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0355906.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IWO52_001633"
     CDS             24845..25585
                     /locus_tag="IWO52_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0355907.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IWO52_001634"
     CDS             25596..26600
                     /locus_tag="IWO52_001634"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0355908.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT         8388 a         4730 c         5521 g         8007 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 tttttt
//
LOCUS       DACEDO010000038        26403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEDO010000038 DACEDO010000000
VERSION     DACEDO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.038"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..581
                     /locus_tag="IWO52_001635"
     CDS             <1..581
                     /locus_tag="IWO52_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0355909.1"
                     /translation="LAPTESAYVWKEILQDLKDRGLKEVLLVVTDGLSGINDSIHSVY
                     PNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEEANNQIRFMIDKWKKQ
                     YPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKYTKRKEQFPNEESLER
                     FLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1054..1608
                     /locus_tag="IWO52_001636"
     CDS             1054..1608
                     /locus_tag="IWO52_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0355910.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1781..2254)
                     /locus_tag="IWO52_001637"
     CDS             complement(1781..2254)
                     /locus_tag="IWO52_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0355911.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            2266..2802
                     /locus_tag="IWO52_001638"
     CDS             2266..2802
                     /locus_tag="IWO52_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0355912.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2842..3213
                     /locus_tag="IWO52_001639"
     CDS             2842..3213
                     /locus_tag="IWO52_001639"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0355913.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            3497..4768
                     /locus_tag="IWO52_001640"
     CDS             3497..4768
                     /locus_tag="IWO52_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAQ0355914.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            5013..6038
                     /locus_tag="IWO52_001641"
     CDS             5013..6038
                     /locus_tag="IWO52_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0355915.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            6332..7441
                     /locus_tag="IWO52_001642"
     CDS             6332..7441
                     /locus_tag="IWO52_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0355916.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            7524..8420
                     /locus_tag="IWO52_001643"
                     /pseudo
     CDS             7524..8420
                     /locus_tag="IWO52_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            8436..9260
                     /locus_tag="IWO52_001644"
     CDS             8436..9260
                     /locus_tag="IWO52_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0355917.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9292..10602
                     /locus_tag="IWO52_001645"
     CDS             9292..10602
                     /locus_tag="IWO52_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAQ0355918.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10750..12009
                     /locus_tag="IWO52_001646"
     CDS             10750..12009
                     /locus_tag="IWO52_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0355919.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12019..12891
                     /locus_tag="IWO52_001647"
     CDS             12019..12891
                     /locus_tag="IWO52_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0355920.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12903..13733
                     /locus_tag="IWO52_001648"
     CDS             12903..13733
                     /locus_tag="IWO52_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0355921.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13773..15956
                     /locus_tag="IWO52_001649"
     CDS             13773..15956
                     /locus_tag="IWO52_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0355922.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16017..17237
                     /locus_tag="IWO52_001650"
     CDS             16017..17237
                     /locus_tag="IWO52_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAQ0355923.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            17234..18694
                     /gene="gtfA"
                     /locus_tag="IWO52_001651"
     CDS             17234..18694
                     /gene="gtfA"
                     /locus_tag="IWO52_001651"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAQ0355924.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18771..19877
                     /locus_tag="IWO52_001652"
                     /pseudo
     CDS             18771..19877
                     /locus_tag="IWO52_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            20199..20408
                     /locus_tag="IWO52_001653"
     CDS             20199..20408
                     /locus_tag="IWO52_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0355925.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20401..20550
                     /locus_tag="IWO52_001654"
     CDS             20401..20550
                     /locus_tag="IWO52_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ0355926.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20585..21369
                     /locus_tag="IWO52_001655"
                     /pseudo
     CDS             20585..21369
                     /locus_tag="IWO52_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(21653..22738)
                     /gene="xerS"
                     /locus_tag="IWO52_001656"
     CDS             complement(21653..22738)
                     /gene="xerS"
                     /locus_tag="IWO52_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0355927.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23072..23305
                     /locus_tag="IWO52_001657"
     CDS             23072..23305
                     /locus_tag="IWO52_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ0355928.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23302..23709
                     /locus_tag="IWO52_001658"
     CDS             23302..23709
                     /locus_tag="IWO52_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0355929.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24846..25364)
                     /locus_tag="IWO52_001659"
     CDS             complement(24846..25364)
                     /locus_tag="IWO52_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0355930.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         9012 a         4023 c         4895 g         8473 t
ORIGIN      
        1 gtcttgctcc aactgaatct gcttatgttt ggaaagagat tcttcaagac ctaaaagacc
       61 gtggtttaaa agaggtttta ttagtcgtaa ctgatggttt aagtggcatt aacgatagta
      121 ttcatagtgt ttatccaaat gctcaatttc aacaatgttg tgtccatata tctagaaata
      181 tcgctcataa ggttcgtgtt agtgatcgac aagaaatctg tagcgatttc aaattagttt
      241 atcaagcaag ttcaaaagaa gaagcaaata atcaaataag gtttatgata gataaatgga
      301 aaaagcagta tccacgagtg gttaaattgc tcatgaatcc tgctatatta accttttata
      361 atttccctcc gtcaatcaga agaactattt attcaactaa tttgattgaa ggttttaaca
      421 aacaattaaa gaaatatacc aaaagaaaag agcaatttcc taatgaagaa tctctggaga
      481 gatttctagt atctcaattc aacaactata atcaaaagtt tctatgccga gttcataaag
      541 gttttaaaga aatacatgat acattagaat caatgtttta acttgatcaa cagaatgggc
      601 ttttccattt acacaaaatt attgacgctc ccgacaaaat tatgatcttg cgtaaagtta
      661 taaaatatta ataaaaatat tttattaatg aaaagcttgg tagcacggtt attcatatgc
      721 taatttattt cactttatag taaggggagt tcttagtata ttgttcaaac ttaaatttca
      781 tataatttaa ttatgtaaat tagctattct aagaacttct acggataact gcccgtctaa
      841 aatttaaata agagcttaga atcttcatat aagtaaggtt aatttttttg tacaaaaaag
      901 tcggaatatt ctgagttact gaaatgatat aaaatcataa actaaatccc ttgattttat
      961 tgactttcaa aagtttatat atataagtta atgattatgg taaaatatat acataatcat
     1021 taactcagaa tatttttaag aagggaaaca atagtgagct ataatgtaca accgttaagg
     1081 acaaatgaag agatacaaga ttttcagttt tggctaagaa gaacaagtaa tccggaaaga
     1141 gatagttttt tatttttgtt tggaatcaat aatgggttac gtatgtcaga tattatagga
     1201 ttaaaagttg gagatatacg aggcaaaagt aagcctatta ttgttgaaca taaaacaggt
     1261 aagcgaaaac cgatttttat agataatttg agagaagaaa ttttattata tactgaaggt
     1321 aaagaagaaa atgattggtt atttccgagt cggcaacaag gtcgacatat aacaagggat
     1381 cgtgtctatc agatatatgc agatatagca gaaaaattag gacgagatga tataggaaca
     1441 catacattaa gaaaaacttt tgggtatcac tactacaaga aaactagaga cattgctacc
     1501 ttaatgttta tttttaatca tagctcgcaa gcaattacaa aaaggtacat tggtataact
     1561 gaagatgaaa taggagcatc acttcgtgga tttaaactag gtgtataatt taaaaatttt
     1621 tataaacagt aaagacaaag attaaaagct atttttatct agaacgattt tatttcataa
     1681 agttcgcgga ataaaagctg gggttactgg tacttggtat aggttcgtgt tatataaact
     1741 aagtaattaa ataacatttt taggttggaa aaggatgaaa ttaaaaaaag agaatcaata
     1801 cggtctcaaa aacgcaaacg ctgcggaata agaaaaatac tgataactca acgtttagca
     1861 gactatctaa tagggatagt ctgctttttt cgtttttgac catacttttg accttctgca
     1921 aacttccttg cttttcctct gtagaactca tgaaatctgc aaaacgaatt gccgtttcct
     1981 ctgtctgctt tttcgcgata tgggtgtata tatcatccat agtggtttta atatctgtat
     2041 gtccaagtcg aacatacact tctttgacag acgcaccaga ttcaaagagc agactacaat
     2101 gggtatgacg aaaaccatga agtgtgatat gttttaagtt attctcttta atgatgattt
     2161 tcaaattgtg atttacatag tctaaataaa ggggcttatt ttcttctgat gaaaacaaaa
     2221 gttgttcacc atcttttata ggtttgattc tcatttttag atattatgcc cgcacgtatt
     2281 catcacattt ctattgtgaa tcgctttatt cgaccgacat ttgactttta tcataatata
     2341 ctcggtttaa aattactaat gaaaacaatt aatcaagacg atcataccat gtatcacctg
     2401 tttttttcag ataatcacca ccgtgtggga actgaactga ctttttttga agtacaagaa
     2461 gggaacaacc agttttttgg aaccaatacg attgaacgaa cgatactaaa agttccttcc
     2521 gaagcatcat tgcatttttg ggaaatatat tttgaaacac aaggcgtttg ccattatgga
     2581 atcgaatcat tttctggacg acctatctta cgttttgaag ggccagatgc aacacgatta
     2641 gctttagttc ccttacgcga atttgaagat attgatgatt atttcccgta tgtcacagac
     2701 cagatcccta cagagcatgc cattttagga attgatgcca ttcaactacg cgtacaatat
     2761 tccgatgcga ctgaacgaga gttattgtcg gtaggctggt agcacaaaga atacgtcagt
     2821 ttcttttcgg gtcatcaacg agtgacaatc ttagaaaatc aagacactca gttctatcaa
     2881 gaagttcaga ttattcaaga tcgtgagaat ccagttgcca ttgaagggat tggtagcgtc
     2941 catcatgttg cgtttggcgt agaaaataaa tctgatttac aacgcatcga caaacaatta
     3001 caagagcgca attttataaa ttcaggaata aaagatcgtg aattttttgt atcactttat
     3061 tatcgtgacc caaatcaatt attgattgaa attgctacag gggaaggaaa cctagatgca
     3121 aaagcgtatg aaaatcaaag tccacgcttt gaagaaattc ctttattttt accgcagtac
     3181 ttaaatttca ttcgtgaaca agtggagcaa taattagcta atcaaatcca tgacgaagag
     3241 taagaggttg actcataagt caaaaaataa agaaaaacgc atcaaatcca ttctaaagaa
     3301 tgttgatttg atgcggttcc tatttttata aactatcatt ttcgactaat aaaactactt
     3361 atgagtcagc ctctcttttt ttcttgattt ttatacctct ttctaggtaa actaaacatc
     3421 tgccaaaaat accgtgtctc attgaaacat tctcaactat ttcaaaataa aggggagtac
     3481 caaatgaacg agtatattga aattatccac gcaagagaaa caacttaaaa atgtcagtct
     3541 caaaattccc aaaaataaaa ttagtatttt taccagcgta tctggttcag gaaaatcatc
     3601 gattgttttt gatactatta cccaagaagc tggtcgtcaa ctgaatgaca cctatgatag
     3661 ttttacccgt ttatttttac ctaaatacag tcgtccagag gttgatgaga ttgataatct
     3721 ttctacggcg attgtggtca atcaaaagaa gattggtggc agtgcgcgct caacgttagg
     3781 gacgattact aacatcaatg cgttatttcg tgtgttgttt tcatgttttg caacacctag
     3841 tttaggatat gcaaatgctt attcatttaa tgatcctagt ggaatgtgtt ctgtctgtca
     3901 gggaatcggt aagacgattg catttttaat aggattgctt tatttttcgg ctattgataa
     3961 tgctgatttt gtattttctg atccatttac cgcagaagtt ttgtctggaa ttgaggaaca
     4021 ggcaaagaat catggttatt ttgtattggt gcataatgtt aattcagcag aagatgttaa
     4081 aactattcag agaaattggc gttttgaggg gttcattgtt gttggtgtac gtgtaaaaga
     4141 atttttggaa ttaaataacg tttttgatgg tcctgttgtt tatattgata cgcatctttc
     4201 aaaagagaca ttaagagaac ttgaacaaca aatgaatcgt ttatttgtaa atacaaatga
     4261 ttaccaagct agtacattgg cagtggaaca tttgattcat tatggtcatg agaaaatagc
     4321 gtttctttct tttgactaca ttgtaggtga accaagtgtt attcaagaac gacatcaagc
     4381 ttatatcgat acattaaaga aatatcattt aacctttaaa tcgcattgga tatataacaa
     4441 ctctgaattt gagcgaattt atcaagaatt agctgaattt actgcaattg ttgttactgg
     4501 agattattta gcggctaaat tagtaaaatt tttaaaaaat aagaataaac gagtgatgga
     4561 acaattatcg attattggat ttgatgatat tacttttgca gaattaatgg atcctgcgtt
     4621 gacaacaata cgactaaatc caggtaacaa gggggcgatt gcttttcacc aactgattgg
     4681 actggccaaa caagaagaaa taaaaaatca agtactacta ttagagggag aattgatttc
     4741 tcgagactct gttcacaaaa tcaaataaaa aagctctgtg aaataagtat agttaacaat
     4801 aaaatatccg aacgatagag agaatgaact gtgatctagc agttcctcgt cgcaaattca
     4861 ctagaattag cattatttac tctaaattgt tcggatattt tttcttgttt ttaattatga
     4921 ttacagtgcc tatttttaac gaaatttgct tattttcaaa atgtatgatt atatgttagt
     4981 aaacactttc aaatatgaaa agaggagcta ttatggtagg aattagggat gttgcaaaac
     5041 gggcgggtgt gtccattgca acagtatcgc gcgtgctaaa tgaggatccg acactttcag
     5101 taaccgatga aaccaaacga aagattgcag aatcggttaa tttttatcat tatgtcaaaa
     5161 aaagtcccgt tgcaaaaaat ttaacaaata ttgcattaat aacgacagtt tctgaagtag
     5221 atgagttgga agatccctat tttcgaacga ttcgcagagg gattcaagtc gaagcagaac
     5281 aaagcaaagt caatttgaag aaaattattc gtttgtcaga aacaccgttg gagcaagacg
     5341 atttgctaaa atgtcaaggt gtgttgatta ttgggcaggt gctatcttct gtgattgaag
     5401 aagtatcagc aattaattcg aatgtcgtag tgattgatga ccctagtatt gaagctgaag
     5461 tagatgcagt ttatactgat ttaacaaaag caacgaatca gcatttagat cgattgtata
     5521 aaaaaggaca ccgaaacatt gcgtttatcg gaggaggacg tgttcgttta gatagccaag
     5581 gaaataaaca cgtgtcagat gatgattatc gaaaaatagc ctatgagacg tggatgaaac
     5641 aaaaaggatt agaaaaatat agtcaaattt atttagaagg ctggaccaca cttgacggaa
     5701 tgcaggcagc agatttactt ttgaaaaaac atgctaaaga taagttgccg actgctatca
     5761 tggtaggaaa tgatcctatt gctgtggggg tttatcgttc acttcaaaaa aatggtctac
     5821 atattccaga agatatttcg attgtgagtt ttgataatat tgaagttgct gaatttttaa
     5881 cacctgcatt aaatacggtg aatgtgaata cagaagaaat tggaaaacta gcagttcgca
     5941 tggttaatga acgaataaaa gaagttagaa atgtttcgat caaagtgatt gtttctaata
     6001 atattattgt tagagaaagt gaaaaaaata tcgattaaaa aagtaaacat ccaaaaaagg
     6061 gtgtttactt ttttgtttac taatattttt agtaaaaaat ctcgacaaaa cattaagtaa
     6121 acagttatgg tgttgttaac aaaaagaaaa cgcattcaaa acaggaggaa ttaagatgaa
     6181 acatttcaaa aaaatagctt taacagggat agctgtttta actgggacat tactcgcagc
     6241 atgtggtggg ggagatagta attctaaaga cgggcaagta actttaagct atgcgagttg
     6301 agatactgac caagctccag gactaagaaa aatgttagat gaatttgaaa aacaaaatcc
     6361 tgatatcaaa gtagaaatgg agacaacgcc ttgggatcaa tattgggtta aattagaagc
     6421 tgcaacaacg ggaggtaata tgccagatgt agtaaccatg cactctagcg aatcctataa
     6481 atatatgtca cagaatgctt tgatgggact tgatgatgtt atcaaagaaa atgatattga
     6541 catgggaaac tttactgaag gaattgctga tttctatacg cttgaagacg aactatacgc
     6601 aatccctaaa gatgcttctg tagtggggct atggtacaac aaagaattat ttgataatgc
     6661 aggtgtcgat tatccagatg aaacatggac atgggaaaca ctcagacaag ctgcaattga
     6721 tttaacagat gaatcaaaag gcatctatgg ttttgcatca catattggaa cagagactgg
     6781 ctactggcca tttatttatc aaaatggggg agaagtattt gccgatgaca atacaaaatc
     6841 aggtttgaat accccagaag cacaagaagc tttagctttt tatcgagata ttgcacttgt
     6901 tgatcatgct tcaccaacaa ttacagaatt acaagatcct gataagttaa gtcgttttca
     6961 atcaggtaga ttagcaatga tgattgaagg aaactggttt gcaccgggat ttttgaaaaa
     7021 tgactatcta gttgaaaatg ttgcttctgc cgtgttacct aagggaaaag ttaatgcaac
     7081 aattacaaat ggacttggtt gggctgccag tgcgagtaca aaatacccag aagaagtgaa
     7141 aaaactaatg gcatttttag ctagtgagga cgccaataaa atccaagcgg aaacgggggc
     7201 atcaatccca gcactaaaag gctttgaaga cgagtgggct gcttcttcag atacaattga
     7261 tttgagtgct tttggaaaaa tgttggagta tggagtccct cgtccataca ataaagatgg
     7321 attacgagct gaacaagcag aagttcaaat tattaaccgt attctgtctg gtgaagtaac
     7381 tgttgaagat ggaaccgctc aaattgaaaa agaagtcaat gcgatattga gcggaaatta
     7441 aattagttaa ttatacaata tacaggcgaa aggattctat ttcgtctgta tattaatgga
     7501 tgaataccaa aaagggggat attatgaaaa atcgaatttt tggaaaatat cgtaaagatc
     7561 aagtgatatg gggctatatt tttatttttc caacggcatt aggattaatt ttattaaata
     7621 tatggccagc aattcaaaca gcaattttaa gttttcaaaa aacggtaggg ttcggtgata
     7681 ctgaatgggt cggattaaga aattaccaaa aaatgtttgc agacggtgaa gtttttcagt
     7741 ctttattaaa cacattaatt tatgcagtgg tttcgatacc tagtattgtt gtattatcct
     7801 tgttggtggc agttttaatg aatagaaaag ttaaaggatt gtcgatttat cggacaattt
     7861 attttctacc agtcgttgct gcaccatccg cagtcgcaat gatttggcgc tggatgttca
     7921 ataatgatta tggactgatt aataacatgt tggctaaaat aggtctcgat ggaccagcat
     7981 ggttaacgga tccatcgatt gcgatttatt ctattatcat tgtaggagtt tggagtgcta
     8041 ttggttataa catggtgtta ttactagccg gcttataaga aattcctaaa gattattatg
     8101 aagcagcgga aattgatggt gctagtccaa tccagcagtt tttcaacatt acgttaccat
     8161 tagttacacc aacattattt tttgtagttg taacaacagt tatcaatgct ttccaagtat
     8221 ttgatgttat ttttatgatg attactccta atagtaccgc catgtttaaa tcacaatcat
     8281 tagcctattt attctataaa cattcattta ttttaaatga caagggatat ggttcggcaa
     8341 tcgttatgtt ccttttagtg attatattaa ttattactgc aattcaaatg aagttgcaga
     8401 aaaagtgggt aaactattaa ggaggctatc aaattatgaa aaataaattt ttttcaaatg
     8461 cgccaatcca tatcatgctt attttaggtg gtattgcaat ggcattacct tttatttgga
     8521 tgattttaac ttcagggaaa acgttggttg aatctaccca aattccacct acaattttcc
     8581 ctgagaaatt tcagctaagt aactatcaac aagtacttga tttattacca ttcatggctt
     8641 tttatatcaa tacattttta atggttgttg gacgagtgat tggctcggtt ttattttcag
     8701 caatggctgc atatgcttgt gcgcgtctaa gttttccagg gaaaaatatc cttttcagct
     8761 tagtacttat tcaaatgatg attcccggtt ccatttttat tactccacag tatttattag
     8821 cacaaaaatt gggtatttta aatacaattc cagcattaat attaccaggg gtagttagtg
     8881 cgtttggtac gtttttatta agacaatttt tcttaggttt acctgatgaa ttggaggaag
     8941 cggcctattt agaaggtgcc aatatttgga cgatcttttt caaaattatg ttgccgcttg
     9001 ctaaatcagg attggttgca cttggaatat tcacagcgtt gtttgcattt aaagatttaa
     9061 tgtggccatt gattgtcaat acttcaattg ataagatgcc tttagctccg ggattagcaa
     9121 acttacaagg acaatatgcc acgaacttcc cacaattaat ggcaggttcg ttattagcaa
     9181 tttggccaat agttgtgata ttcatcgttt tccaaagaaa attcattgaa ggaattgcta
     9241 caacaggtag taaattataa aactattaat tagtgattgg agagtaagta catgacaaaa
     9301 attacattta ttggtgcagg aagtactatt tttgcaaaaa acgtattagg cgatgcgatg
     9361 ttaactccaa gtctacaaga tgcacatatt gcattgtacg atattgatga aaaacgacta
     9421 aaagaatcaa atttaatgct taaaaatatt aataaaaatt cgaatgaaaa tcgtgctgat
     9481 attgtttctt attatgatcg caaagaagcg ttaagagagg ctgattttgt tatcaatgct
     9541 attcaagtgg gtggttataa accttcaaca gtgattgatt ttgatattcc taaaaaatat
     9601 gggttgcgac aaactattgg tgacaccatg gggattggtg ggatttttag aaccctacgg
     9661 acactaccag taatgttaga ctttgctaaa gaaatggaag aagtttgtcc aaatgcttgg
     9721 ttattgaact atacaaatcc gatggcgatg ttaaccatgg gtgttttaaa agcgacgagt
     9781 attaagactg ttggtttatg tcatagcgtt caagtatgcg taccagaatt gtttgagcat
     9841 ttaggaatag ccgatcaata tgacttaaat gactttcaat ggaaaattgc aggcattaat
     9901 catatggcat tcttgttgga aattacaaaa aatggggaag atttctatcc aacaattcgt
     9961 cagttagctg cagaaaaacc aaatccacat ccagattctg tacgttttga attgttgaaa
    10021 cgttttggtt actatattac agaatcaagt gaacacaatg cagaatacca tccatacttt
    10081 attaaatcaa aatacccaga attgattgac gaattaggga ttccgttgga tgaatactta
    10141 cgtcgttgtg aacaacaaat taatgattgg gaaagcatgc gagaggacat tgtaaataat
    10201 ggggagttaa cacatacgag aagccgtgaa tatgcttcat atatcatgga ttctattacg
    10261 acaggaactc cgtcagtgat tgcaggaaat gtcttaaata aaggcttaat cacaaactta
    10321 ccgacagatg tttgtgtgga agtaccttgt ttagtcgata aaaatggcgt gcaaccaacc
    10381 tatgtcggtg aattgccaac acaattagct gctttaaatc ggacgaatat caatgttcaa
    10441 gaactgacag tagaagccgc tttaacacta gataaatcaa aaatttatca agcagcgtat
    10501 ttagatccac atctatcatc tgaattgtct ttaacagata tcaaaaattt agtggatgat
    10561 ttaatcgaag cacatgggga ctatttacct gaatataatt aaaaagaagc tgaaagagta
    10621 aggaattttc aattccttac tcttttctta gttaaattta gtaaaaatat tgacaaaaca
    10681 cttagtaaac agttacatta gtgttaacaa aaagaaaacg cattcaaatt gtataaggag
    10741 gagcataaaa tgaagtggtc taaaaaaatt attttagctt gtatagcact aggagcaacg
    10801 atctctttga ctgcttgtgg aaaaaatcag gaagatgcaa gcggtaaggt agaaatagag
    10861 ttttttaatc aaaaaaaaga aatgactcaa acgattcagg agattgctaa agattttgaa
    10921 gcaaaaaacc ctgatattca tgtaaaagtt gtggatgtac cgaatgctgg tgaagtaatt
    10981 aaaacacgta tgcttgcagg cgatgtacca gatgtcatta atctttatcc tcaaagtata
    11041 gaattgaaag aatgggcgaa agctggttat ttagaggact taaccgaaga accgtattta
    11101 gagaatatca aaaatggata tgcacaacgt tttgctattg aggatcgtgt atatagcata
    11161 ccattaaccg ctaatgtcta tggtttttac tataacaaaa ctgcttttga ggaaatgggg
    11221 attcaagcac cagaaacttg ggcagaattt gaaaaaatag tagctgagat taaagatcaa
    11281 aacaaagtac cgtttgcgat tgcaggagca gaaggctgga ctttaaatgg ttatcaccaa
    11341 ctagctatag cgactgctgc tggtggtgaa gaggaagcga acaatgtttg gcgtttctca
    11401 gaagttaatg gaattaatgc tgaaagcaaa gaaatgcaaa aagattttaa tcgattagat
    11461 ttgttaagag aaccaaaagc tttgcaaaac aactggcaag gtgccggtta taacgataca
    11521 gtagttactt ttacaaaagg tgatgcgttg attatgccga atggttcatg ggcaatgccg
    11581 atgattcatt cacaaaatcc agattttgaa gtaggaactt ttccttttcc agcagatgaa
    11641 gcaggtcaaa gtctaacgat tggcgcagga gacctagcct tatcagtttc tgcaacttct
    11701 caacataaag aagaagccaa acgttttgta gaatatatga caacacctga agcgatgcag
    11761 aaatattatg atgtagatgg tgcaccgtgt gctgttgagg gcgttatcga gaatactcag
    11821 gattcaccat taagtggttt aactgaactt gcttttactg atcgtcattt agtttggtta
    11881 gccaaagatt ggaatagtga aaatgatttt tataccttaa caacaaatta tttacacaat
    11941 ggcaatcaaa aagccatgat taacgcatta aatgctttct ttaatccaat gaaagctgac
    12001 ggaaggtagg aaaataaaat gaaaataaaa gaaattttaa atcgttactg ggcaatcatt
    12061 tttgtagcaa ttccaattat tttgcagatg atttttttct actttccaat ggttcaaggt
    12121 gctttttaca gtttaactaa ttggactggt ctgacttata attttgattt tgtgggtttg
    12181 aacaattata agattctttt ttctgatccg aagtttatca aatcgattgg cttcacttta
    12241 attgtgacgc tttgcatgat tgctggacaa attggttttg gtattttgat tgcgcgggcg
    12301 ttgaacgcaa atattaaagg gaaaacattc tttagagcat ggttcttttt tccagcggtc
    12361 ttatcgggcg tggttgtttc tttagtattt aaacaaattt ttaactatgg tttaccaaaa
    12421 attggtagta tgttaggcat tgaattttta caaaccagtc ttttaggaac aacatgggga
    12481 gcgatttttg cagtaatttt tgttttactt tggcaaggaa ttgccatgcc agtcatcatt
    12541 ttcttagcgg gttttcaaag tattccacaa gaaattattg aagctgctag aatggatggg
    12601 gcgaatgata agcaaatttt ttggaatatt gaaatcacct acttattacc aagtatttca
    12661 atggtattta ttatggcatt aaaaggtggc ttaacagcat ttgatcaaat ttttgcttta
    12721 actggtggtg gaccaagcga tgccacaaca tctttggggt tacttgttta taattatgct
    12781 tttaaaaaca atcagtttgg ttatgcgaat gctattgctt tagtcttatt tatcttaatt
    12841 gggattgttt cactggcaca attaaaatta tcgaaaaaat ttgaaatcta ggaggtttcc
    12901 caatgaaaac atcaaaagaa aaaaatattt ctaaatactt gcttttatta ttagggggct
    12961 tcatgatttt agttccctta gtttcaacgc tattcggttc atttaaaaca acacccgata
    13021 ttatgaataa tttctttcgt tttcctaatc cagcgacttt ggaaaatttc cgaagattgt
    13081 tagcggatgg aattggtcat tacttttgga attcggcaat catcacaatc attgcagtag
    13141 ttgctgtgac agcaattatt cctatggctg cttattcact agcaagaaaa atgactcgtg
    13201 ggaaagcata cggattgatg tatttatttt taattttagg aatttttgtt cctttccaag
    13261 taattatgat tccgattacc gttatgatga gtaaaattgg tttagcaaat atgtggggat
    13321 taatcttact ctatctcact tatgccgtac cacagacact atttttatat gttggtttta
    13381 ttaaactaaa tgtgcctatc agtctggatg aggctgctga aattgatggg gctagtcact
    13441 ttacaactta tttcaaaatt gttttcccaa tgttaaaacc aatgcatgcg acgactatta
    13501 ttcttaatgc tctatggttt tggaatgatt tcatgctacc attattaatt ttaaatcgtg
    13561 attctaatat gtggacacta ccattgtttc aatataatta tacgggacaa tattttaacg
    13621 actatggtcc aagttttgct tcctatacag taggaattgt cacgattaca ctagtttatc
    13681 ttgctttcca aagaagtatt atagctggaa tgagcaatgg agcagtgaaa taaccaataa
    13741 attattctca ttataaagga gttcataaac ttatgattat atttgacaat cacacacaaa
    13801 catttcattt acaaaataaa aaaattagct atctattagg aatcgaagaa aataattatt
    13861 taacgcatct ttattttgga aaaaagatta cgcactattc tggtgggtat cgctaccccc
    13921 gaacagatcg ttccttctca cccaacaatg caaatgcgca agatcgttta ttatctttag
    13981 acacattacc attagaattt ccaggatatg gacatggtga ttttagagaa ccggcgcata
    14041 atattaaatt aggaaacggt agtcgtgtaa atgattttcg ttatgatagc tacgaaattc
    14101 aaaaaggaaa gcccagctta gcgggattgc cagccttata tacggaagat gatgatgaag
    14161 ctgaaacgct gattattcaa ttgaaagacg cgattagtgg attgacttta cagttgttct
    14221 acacaattta tcaaaatcac agtgtaatta ttcgttctac gcaattagta aataatagtg
    14281 atgaaacagt ggagattaac cgcttagcta gtcaatcaat tgattttcca aatcgtccac
    14341 tcgagttaat tcatttaaat ggtacctggg caagagaacg tcaaatgact agagaaaaaa
    14401 ttcatacagg gataaaagta ttagatagta aacgcggatc aagcagccat caacaaaatc
    14461 cgtttgttgc tttagtagat gccaaaacag atgaatttca aggagaggca tatggattta
    14521 atttagttta tagcggtaat cacgaaacgg tgattcaaaa agatccattt gaccaaacac
    14581 gggtcattat gggaattaat tcatttaatt ttggttggca actaaaaact ggcgactcat
    14641 ttcaaacgcc agaagtcgtg atggtttata gtaatgaagg tctgaatacg ttatcacaga
    14701 cgtatcatga attgtataat caccgtttaa ttcgtggaaa ctatcgttta aaagaacgtc
    14761 ccacgttaat caacaactgg gaagcaacgt attttgattt taacgaagaa aaaatcatga
    14821 atattgtcga tgatgcgaag aacttgggca ttgaaatgtt tgttttagat gatggttggt
    14881 ttggcggtcg tgaaaatgac ttcacttcat taggtgattg ggttgaaaca gaaggcaaac
    14941 ttgcttgtgg tttggaaaat ttagccaagc aagtgcatga taacgggatg aaatttggga
    15001 tttggtttga accggaaatg atttctgaag atagtgaact atttcgagca catccagatt
    15061 gggcattggg tattccaggt cgtgaccgct caattagtcg aaatcaatat gttcttgatt
    15121 tttcaagaca agatgttcgt gataatatct atcaacaaat gacggcgatt tttgatcgtg
    15181 taccgattga ttatgtgaag tgggatatga atcgaaatat gacagaagtc tattctgtct
    15241 tgttggactc aacgatgcaa ggtgaagtgg ctcatcgtta tatgttaggg ttatatgaat
    15301 ttttagaaaa gttaacaacg aattatccaa ctattttatt tgaaagttgt tcgggtggag
    15361 gcggtcgttt tgatgcaggt ttcttgtatt acatgccaca aacttggacg agcgacaata
    15421 ccgatgccgt ggctcgtctg aaaattcaat atggaaccag cttagtgtat ccaatttcaa
    15481 gtatgggtgc gcatgtttca gcagtcccaa atcatcaaac aggaagagaa acgagtttaa
    15541 ctattcgtgg caatgctgcg atggctggtg tttttggtta tgagctagat ctaacgcaat
    15601 taacttctga agaaaaagca gaaattaaag aacaagttgc cttttataaa aagcatcgcc
    15661 aacttttgca atatggaact ttttatcgct tggaaagtcc gtttgaaggc aatgatactg
    15721 catggatgtt tgtctcaaaa gaccaaacag aagccatggt cttttatttc cgagtattag
    15781 cagaagctgc gcatccatta gtcacattga aattagcagg attagcagaa gaagcaactt
    15841 atcaaatgga agatgtaaca tttgctggcg atgaattgat gaatctcggt ttttatattg
    15901 actcagaact acatggtgat tatgcgacac aacgctttta tttgaaacag atatagtagc
    15961 ttgaatttac aatataagtg caacaccaaa agagactcat tccaaacgaa gaaaatatgt
    16021 tttatttaca cgcatttact aaaaagcagc ctgatttgaa ttgggaaaat gaagaagtac
    16081 gtgaagaaat ctatgcaatg attaactatt ggttagataa aggactaggc ggatttagaa
    16141 tcgatgcgat attaaacatc aaaaaaaaac tagagtatgg catctttgaa tctgatggag
    16201 aagatgggtt agccttcatt gggccctgga ttcttaatca gtcgggcatt gaagtgtggt
    16261 taaaagaaat gagagagcgg acatttaaac cacataatag tatgacggta gcagaagccg
    16321 atgttcctaa tgaacgttta gatgaatata ttggtgaaga tggctattat tcgatggttt
    16381 ttgattttag ttatacggat attgatgtac ctgaaactgg tgaatggttt aaagatagcc
    16441 aatggacatg gacggatatg cgtaccaata tttttacgaa tcaattagtt acgcaagata
    16501 aaggttgggg cgccttatat ttagaaaacc acgatcaacc acgttcaatt aataaatata
    16561 ttccagaaga atggatcaat gattatagta aaaaaatgtt ggcgacgctt tttatgttac
    16621 taagaggtac cccttttatt tatcaaggac aagaactagg aatgaccaac atagaaatgg
    16681 catcattaga agattttgat gatgtagcta cgcattctca gtataaacgg gcattagaat
    16741 atggattggc accagagcaa gcattgaaag ctgtcaataa acgaagtcgg gataattctc
    16801 gcacacctat gcaatggaat acgcaaaaaa atgccagatt ctcaacttct gataatgtat
    16861 ggttaaaagt aaatcctaat tatccagaac tcaatgcaga agctcagtta accaatcaac
    16921 agtcggtatt caattattat catcaattaa ttaaattaag aaaaaatagt gtatacaaag
    16981 acgtgctaat ctacggacaa tttctaccag tgaaagataa aaatgaacat attttgcttt
    17041 atgaacgcca attagaagat aaaatcgtat tagttatctt aaacgttaca gcaacagaac
    17101 aagaatatta tgtagatgaa cagtaccaac aagtattaat caataattat gaagatgttt
    17161 tattagatca aagtcgaaaa ttgcgtttac gaccgtttga aagtattgta ttagctaatt
    17221 atggaggaca attatgaaaa ttaaaaacga agcaatgctc atcacctatg cagatagttt
    17281 aggaaaaaac atgggcgaat taaaacaagt cttagacaaa catttacaag gtgttattgg
    17341 tggtgtacat ctgttgcctt tttttccatc gacaggtgat cgaggctttg cgccgagtga
    17401 ttatacgaca gttgatccag ctcttggaac atgggaagat gtggaagcat taggtgaaga
    17461 ttattatttg atgtttgatt ttatgattaa tcacatttct cgtgaatcaa aatttttcca
    17521 agatttcaaa aaaaaccatg agcagtcacc atacaaagaa atgtttatcc gtattcatga
    17581 atttttccca gaaaatcgac caacacaaga agatattgat ttaatctaca agcgtaaaga
    17641 caaagcgccg tttcaaacgg tagaatttgc ggatggtacg acagaagagg tttggaatac
    17701 ctttggggaa gaacaaattg atttagatgt gaccaaagaa gttgtgaaag aatttattcg
    17761 agaaaccatc aaagatatgg cgaaccatgg ctgttcttta attcgattag atgcgtttgc
    17821 ttatgcgatt aaaaaattag ataccaatga tttcttcgtg gaaccagata tttgggaact
    17881 tttagacgaa gtgcgtcaag aagcagcgaa atatgacgtc gaattattac cagaaatcca
    17941 tgagcattat tccattcaaa tgaaaattgc caatcatgat tattatatct atgacttcgc
    18001 cttgccaatg gtcactctgt attcacttta tagtggaaaa tcagaacgtt tagccaattg
    18061 gttgaaaatg agtccgatga agcagtttac aacattggat acgcatgatg gaattggtgt
    18121 tgtggacgct cgtgatttat taacagatga agaattggat tacacctctg aggaattgta
    18181 taaagtcggg gcgaatgtga agaaggtcta ttcaagtgcc aattacaata atttagatat
    18241 ttatcaaatt aacagcacct attatagtgc attaggcgat aatgaccgta gctatctgtt
    18301 agcacgtgcg attcaagtat ttgcgccagg gattccacaa atttattacg ttgggttatt
    18361 agcaggaaaa aatgatattg aactgttgga agcaacgaaa gaagggcgta atattaatcg
    18421 tcattactat gatttagccg aaattgaaga agaagtgcaa cggccagtga ttcaacaatt
    18481 atttgcactt ttaaaattta gaaatgaatc tgcggcgttt gatttggatg gaacgattga
    18541 agtagcaacg ccatcagaat cagaaatagt gattacacgt aaaaataaaa acggtagtga
    18601 tagtgcaaca ttaacggcta acttggatac gaaagaattt atcattgagt ctaaagatat
    18661 aaaaattttc actagtgttg cattaaaatt ttaacctatt ccatagaaca aaaaatcctt
    18721 tatactagca cttggatgac ttatccaaga ccaataaaaa ggactcatac atggataagt
    18781 ataaaacaaa tacagcattt aataaatggt tttctgccat aaaattagga aaactttctg
    18841 tcaactccca aaaatgtatt tttgattaca attcgtatcg taaaaaactg tcttttgaag
    18901 ctgtgctcaa gctctttctc tacgcagtta atgaagaaaa agaaagtttg cgtgatttga
    18961 gtacagctct tatcgaccga tcgctccaac tagaaaccga tgtgtcatcg attagtcata
    19021 cccaattgtc tcgagcattt aatgcacttg aacctaaagt gttagaagaa atttttcaac
    19081 aactgttaga aaaagttcaa catcaaatca aaccaaccaa gcgaaatcat ctgtatctga
    19141 ttgattccag tacgttttcg cttagtttaa aacgtcataa gtgggcgact ttccgtcaaa
    19201 caaaatcagg tgtcaagtta cacttaaact attgttatat ggacgatact acgatgtatc
    19261 caactgattt taccttaaca aatgcccatg aacatgatct caatcaacta ccagtattgg
    19321 tgaatcaacc agaggcaact tacgtgtttg atcgtgggta ttaggacttt gaaaagatgg
    19381 atcagatgca ttgggatggc tatttcttcg tcactcggat taaaaaaaac accaaagttc
    19441 acgtgataga tactctggaa acatcgccag aaacagaaat tctacgagac gagctggttc
    19501 gtttaggatc taaaacctat ctaaccgcca atttccgact agtcactgtt caagataaga
    19561 acggacgtgt gtttcaattt atcaccaatc gaatggacgt ctcttcaaaa gaaatctctg
    19621 atatgtatca cgctcgttgg caaatcgaat tgtttttcaa gcacatcaaa caacacatga
    19681 ccattaaaac cttcttttct caaagtgaaa aaggcgtgca gaatcaattg attttaacga
    19741 tgatttccgc tctcctcacg tttctcatca aattggagac gaaaacggaa aaatcagtgt
    19801 tccaaatcaa acgattcttt cgttatttac tgtttcagcc ctttgaatgt tgtctagaaa
    19861 aattaattcc cacatgagaa aaaagaaatt tggctcgtta cttttggttt tcagttgaat
    19921 aacagataaa aaattttctg gaaaaagtca tcctctgcgt cagcacagtt ttttttgact
    19981 tattttcaga ggaaatagtt ttcagaatat tctaaaatgt tttatgcaac actagtgata
    20041 ttttttagaa ttagatttct aatgtgcatt ttttgagtat taaggagcaa gtatacaaag
    20101 atttataaag ctgattaaat ttgaataaaa atatgcagga acgctactat ttttagtagt
    20161 gttctttttt atatttgaat aaaaaatgtg gtggttaaat ggtggttata acaaaaaatg
    20221 aattaatgaa attaggttat gggaagtatc agtctgagag aatcatacga caggcaaaag
    20281 caattatggt ttcaaaaagc tatacctatt acgaaaatag aagattagga ctagtacctg
    20341 ttgaatctgt agaagaagtt ttaggtatga agatagaggt agaggaggat tttcagaatc
    20401 atggttaatt ttaaaaaaga taaaaatggg aaatatcatt ttactgctag tttgggattc
    20461 gacaaacgaa cagggaaaag agtacaaaaa aacgtagagg ggttaaaaca gttaaagaag
    20521 aaaaaaagcg tataccgaac ttgtttctaa ttatggaaaa gaagcagaaa cttctcaaag
    20581 tactatgttg tttgaggagt ttttttataa attattccta ccttattatc aagggaaagt
    20641 agaagaaaga acatataaaa atcggttatc tacaatcaaa aaaacttttc ttatttcttt
    20701 aaaatggagt taagagaaat acgtccaatt gacattcaaa agtggcaaaa taatttattg
    20761 agcaaatata aaaattctta tgtaagaaac atgtatggga tttttcagat gtcattagat
    20821 cgagcagttg ttttgggact acttgaaaaa aatcctgcaa aagttattgg aaatgtgaag
    20881 aaaaggaaaa ctacagttga tttttggaca aaagaagaat ttacaaaagt tatttcaact
    20941 ttttatattg atgatttgta ccaacatttt ggttttattt gtatgtggtt actttttatg
    21001 accgttatgc gtttgggaga agccactgca ttaacttgga aagatattga ttttgaaaat
    21061 aaaaacttgc gtgtgaataa aacactctat tataagagag ccaaaaccta tgagtttgta
    21121 gaacccgaaa cggcttctag tattcgtaca atcgaactag acgacaatac aattaccttt
    21181 ttaaaagctt ggaaggagcg tcaagtttca ataggaggca tgtcgtttgt actttcttat
    21241 aatggaacac ctactcagaa atatacaatc cgtcatatta tgagaagaca tgcaacgatc
    21301 gttggtgttc atataatccg catacacgga ttaaggcata gtcatgcttc tttgcttata
    21361 tcacaataaa aagatgcact tcgaattaaa gaaaggttaa aacatgctga tgtacaaacg
    21421 actttaggaa cttatggtca tttgtatcta aattcattga gagagataac aaatgatccc
    21481 acagattata tccaaactaa tttttctaaa atcaaaccga cacctattta cgataataaa
    21541 aaataaatga taaacaaaag taggatcaca ccaaaagaaa cacactagaa aaactataat
    21601 acaggctttt ctagcgtgtt gtttcttatt tccgcctatt tatacattat actcataaag
    21661 catttaatgc atcgatcgta gccgcattgt ctatatgggt atataaagtt gttgtattgg
    21721 tacttgaatg tcccaattgt tgggcagtta ataccaatga atttgtctgt tggtagagtc
    21781 tagtagctaa tgtatgacgc agtttgtgag ggctgacttg aattctaaag ccttttgaat
    21841 attttgctac catccgttcg attgcaatac cggatatacg cttggcttct ccttggtaaa
    21901 ttgctagaaa taaagctttt tcgttttctg ttactttata tcgttctttc cgaatagcta
    21961 gataattagc taaatattcc attgcgaatg ctgctatatt gacagcatct cttttatttc
    22021 cttttctagt tactaccaca ttattcttat ctaaagatag gtcatctaaa ttaatattta
    22081 taacctcgga gagtctcaat ccagatccta aaatcagtgc attgatcgcc aggtctcttt
    22141 ctacatctcg ttgataatag atcaaagctc gtttactcaa agtattttta tattcattgg
    22201 caatataatc tagataattt tgtgtatcct tatctagaaa taattttttc tcaatggcat
    22261 tggcacgtgc agctaacgta gttttatctg tgatggttgc tacttgatcc ataggattat
    22321 tgtctaagta tggttttcct agaggattat tggaactttt cgaaagatat ttaaatagaa
    22381 cctttaatgc cgtgatattt ctattgatag tagaagtaga tagacctaag ttattattat
    22441 ctttgagctt agtttttgtt gtctcttttt ttacattttc ttttgtgcga cgtttcaaat
    22501 gagatttgta caagtttagg tcataggcag ttaactgtct tatcgaggtg atagaagtat
    22561 caggaacgat agactcactc agaagccatt cataaaagag cttataattt tttagatatt
    22621 ctaaagtggt tcgaggtgat tgatctttat cctcacgatc ttgaacaaaa tcttttatat
    22681 aatcgggcat atgaagtatg attgtatcta ttttttgata tatttctgta tttttcatgt
    22741 cattcatcct ttcaataagt aggggagaag agtatctcta taattggact ataccaatta
    22801 tagaatgttt tgtcaattgg tatgctctaa aatttattaa tttgtttcaa aaaaagcata
    22861 taaacaggta tacgtttaat aatttaaaat aaatggtata cataaatagg gaaggaaata
    22921 gaagggagca atctaaattt tttgaatttt cttaattaat tatatagaaa gaacaatctt
    22981 tctatagagt tagacatgag agggactatg cttgatttaa aatcaacaag cgtatatact
    23041 gtatatacaa agaaaaagga gggagaagac tatggcaatt aaagaaagaa aatttaggga
    23101 agtggggaac tcagtagtat taactatttc aaaggagttt cttaaagaaa atgggttaaa
    23161 tcccggagat acggtttttt tagatgaaga aaaacttcga gcggcaatta caaaaaaaca
    23221 agccttatct gatgaaattg atttagcaat atctcaagca ttaactgaat atgatactac
    23281 ttttagagag cttgtcgata aatgagtatt cattacctag aagccaaaga tattataaaa
    23341 atgaatgtaa tccaaataaa aaaatactct cctaatgaac cccttggtgt aaaggatgcg
    23401 aatgctttag aaatgtgtgt agggcaactt cgagcctcag cttttggtga agaggtttat
    23461 ccttcgattt atgaaaaagc agctatccta cttatacaat tgataaagaa acatcctttt
    23521 cacaatgcaa ataaacgtac tgctttctta gctacttttg tctttttgaa aatcaatgga
    23581 cagcttttag ctattgatca aaaagatgct gtaaatttag cggtatatat tgctacgtat
    23641 gataaggatt tcgatcaact aaaatatgaa gtgatcaaca ctataaagac acatacgcag
    23701 cccatttaag gagttactgt atgtgtcttt atagggttat tagctgctat agcgaaataa
    23761 ttgaagtaaa ttacaaatag ctataattca aatccaacta tatagaaaac gtaaattttc
    23821 tatatagttg gattaagata acgactattt attttatcat atttttacta taccctatac
    23881 ttttacttgg tatatttatg aataatcgca aatttacccc tatactacta gtttgtaagt
    23941 tagattaata aaaatactaa agttatttgt tcttttatta aatacaccta caacttataa
    24001 attgatggta tttagataac acgaaagaat aaaaataaca gctgatagta aagtttgttg
    24061 acgattgcta ctcaatcgaa agtgagttag tgcgtatgaa cttgcttctg aaaacttatg
    24121 aaatgaaaga ccttttacat cttgcagaaa tcggtactgg gattaaacta agcaataaaa
    24181 actaatgttc ccctcattct ttgaacttct actatttgat ttatttattc tgataaatga
    24241 cgatttcacc ttcaggatct tcaaatcccg tatagaattt atttaagatc tcttcagtat
    24301 cagtaatagg ataggcgata ggccacatac cctttgatgg ttagaataaa ctgctcaaaa
    24361 tctgtatcgt atgttggctt tagcatatta acaaaggcta tctccaaatt ataaaccata
    24421 agcgttgaat cgttcttaac aatttcaatt cttcctaacc caaagtcaga agatataatt
    24481 accggacttg ccttaccctt catgtgggtt gttgagattc catatttaaa gggaattgtc
    24541 ggtactatcg gcggtctgaa aaacatccca aaaataaaaa aatctttccc ctgctagtct
    24601 cctaagtccc tttgtagcgg atatggcacc ataatctcat tttatcgaag tgagtcatgg
    24661 tttatctgtt ggccaaccaa aattgataca atctaacgat gcaccctcta cggcattcta
    24721 ggggagtgca cttgcattca cccaataaaa accggcagtt agcaaagaac ttatgtagtc
    24781 gtatgttctt actgttaaca tacaagaggt ctcctttcca caattgctta gcagtaaacg
    24841 ggaatttaca aagctaatct cttccatttt cttatctttg ttctaaaagt cccaaatgga
    24901 gcaactgtat ttacatgaat aaatttatat acttcccata cagccgtttt ggttgcctcg
    24961 tctgcccatt ttcttttgtg tgcagtgaat agatcttcat cactcattga atcaatcatc
    25021 atgttaatat catcaatatt acgattgaga atttcctcta attctttcaa tggtagatgt
    25081 gcataggtat ttgtaaacca cttatataac tctcctaact gattccattt aaattgatca
    25141 gaaggcgttt taacagataa tccatttctt tcatcatttt cccattttag aattaaattc
    25201 gtccaaccaa cttgatatgc taaattttca gcaggtgttc tatccacttc ctcaactctt
    25261 ttatccttca attcttcagg aatagtttca aattctaaaa tatatttagt ataagtccta
    25321 tttatttctt tttttaattc ttctttattt ttatatgttc tcattatatt acctccataa
    25381 attcctaatt gtctccttta attcatctat aatttcatcc acgatatttt aatccgttct
    25441 acaatgcata tctgatatgc attgactgtc taactcatat gtgaatgatt tataaaatat
    25501 cccaacgtct catctaaaga aatgttgatc gtcagcaagg agctaaaaaa tacgaatata
    25561 tttcttttgt agtaaaatcg gactggtttg catatcattt tcttcgtaaa tatcaaaatc
    25621 atgttaacta ctatcaatta ctaataaaaa ctaccacttt aagcgttaag aactgttaag
    25681 tttagttaag tgaggatagt tcacaaaatc cattgttaaa cttcacgaaa gtggtaaaac
    25741 tcaaaatgct ttatatagga ttacagcatc gctctttctt cgattgcgcg ttgggtaaaa
    25801 aatattatga agtaaaaatt gacgatgaca cgttccttac ggctcatcag atgaaacagc
    25861 tacaaaaacg aaatgatcaa ctcgaagaaa atttaattct aaaaaagcga ttgccatatt
    25921 cacgcctcgc tcgacgaact attaaaggtc aagatttacc tgaagatcat cctttttagg
    25981 aaattgaagc tttttatgtt gcacaatgca tttataatac tacaatgatg ttttcaccag
    26041 atattcttct agggtgcggt gtaatgaaac agatacactt gcaaaagaaa attcaccgcg
    26101 aatttactaa aattatgaat aattatgtta atcttccgaa atttttttcc aaaattaagg
    26161 gatcaagctg gtgttataga ttgtttaagg ttggcaaaaa aataacttct ttttaaacaa
    26221 ttatagaaaa ccgatactct gttaagttgg ttgacacggt gtcgattttt atgactttct
    26281 tattctgaaa cttcactaac ggacagtacc taaaaagtct atagtatgga cgtttacgag
    26341 tactatttta tcaaaaatgc tgctcatttc aacttatatg ttttagtgaa ctacccccac
    26401 tta
//
LOCUS       DACEDO010000039        26267 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEDO010000039 DACEDO010000000
VERSION     DACEDO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.039"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(42..1244)
                     /locus_tag="IWO52_001660"
     CDS             complement(42..1244)
                     /locus_tag="IWO52_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0355931.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1361..1876
                     /locus_tag="IWO52_001661"
     CDS             1361..1876
                     /locus_tag="IWO52_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0355932.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2075..2521)
                     /locus_tag="IWO52_001662"
     CDS             complement(2075..2521)
                     /locus_tag="IWO52_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0355933.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2568..4046)
                     /gene="msr"
                     /locus_tag="IWO52_001663"
     CDS             complement(2568..4046)
                     /gene="msr"
                     /locus_tag="IWO52_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0355934.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4400..5029)
                     /locus_tag="IWO52_001664"
     CDS             complement(4400..5029)
                     /locus_tag="IWO52_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0355935.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(5013..5477)
                     /locus_tag="IWO52_001665"
     CDS             complement(5013..5477)
                     /locus_tag="IWO52_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0355936.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5964..7232)
                     /locus_tag="IWO52_001666"
     CDS             complement(5964..7232)
                     /locus_tag="IWO52_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0355937.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7463..8200)
                     /locus_tag="IWO52_001667"
     CDS             complement(7463..8200)
                     /locus_tag="IWO52_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0355938.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8267..8835)
                     /locus_tag="IWO52_001668"
                     /pseudo
     CDS             complement(8267..8835)
                     /locus_tag="IWO52_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            8963..9835
                     /locus_tag="IWO52_001669"
     CDS             8963..9835
                     /locus_tag="IWO52_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0355939.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10177..11049
                     /locus_tag="IWO52_001670"
     CDS             10177..11049
                     /locus_tag="IWO52_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0355940.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11046..13004
                     /locus_tag="IWO52_001671"
     CDS             11046..13004
                     /locus_tag="IWO52_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0355941.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13336..14187)
                     /locus_tag="IWO52_001672"
     CDS             complement(13336..14187)
                     /locus_tag="IWO52_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0355942.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14310..14777
                     /locus_tag="IWO52_001673"
     CDS             14310..14777
                     /locus_tag="IWO52_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0355943.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(15040..15522)
                     /locus_tag="IWO52_001674"
     CDS             complement(15040..15522)
                     /locus_tag="IWO52_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0355944.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15622..16485)
                     /locus_tag="IWO52_001675"
     CDS             complement(15622..16485)
                     /locus_tag="IWO52_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0355945.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17083..17958
                     /locus_tag="IWO52_001676"
     CDS             17083..17958
                     /locus_tag="IWO52_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355946.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(18103..18255)
                     /locus_tag="IWO52_001677"
                     /pseudo
     CDS             complement(18103..18255)
                     /locus_tag="IWO52_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(18523..19854)
                     /locus_tag="IWO52_001678"
     CDS             complement(18523..19854)
                     /locus_tag="IWO52_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0355947.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(20003..20716)
                     /locus_tag="IWO52_001679"
     CDS             complement(20003..20716)
                     /locus_tag="IWO52_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0355948.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            20891..22315
                     /locus_tag="IWO52_001680"
     CDS             20891..22315
                     /locus_tag="IWO52_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0355949.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            22614..23321
                     /locus_tag="IWO52_001681"
     CDS             22614..23321
                     /locus_tag="IWO52_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0355950.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            23308..23631
                     /locus_tag="IWO52_001682"
     CDS             23308..23631
                     /locus_tag="IWO52_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0355951.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(23720..24757)
                     /locus_tag="IWO52_001683"
     CDS             complement(23720..24757)
                     /locus_tag="IWO52_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0355952.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(25078..26061)
                     /locus_tag="IWO52_001684"
     CDS             complement(25078..26061)
                     /locus_tag="IWO52_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0355953.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT         7982 a         5297 c         4603 g         8385 t
ORIGIN      
        1 atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc ttcatttgta
       61 gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat ggcacctgca
      121 aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat aaatggtgct
      181 aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt tcgttgcaga
      241 cctggtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg gaaaaatcct
      301 gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc taataacagc
      361 aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt aaacttccgc
      421 tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca gccaaaaaat
      481 acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata cgcaagtaaa
      541 gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag tttatgattg
      601 cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc agtttcaggt
      661 aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga
      721 taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc cattccgcag
      781 ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa aatactgacg
      841 atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc ggcacgtaac
      901 ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac aacgatgata
      961 aacaatcctg tcatcaatgt ttttttcaaa ccgatccgac tagataagaa acctgtaaat
     1021 agcatcacca caccagtgac taacatgtaa ctagtaaata acagggtcgt ttcagctggc
     1081 gtagcgttta actgtgcagc aatcgttttt aagattgggt ctactagacc cactcccata
     1141 aaagcgatca tacaggtagc tgctacaact agcgctgaac ttttcccctt tgtttcttca
     1201 tttgtttgtt cttgtatatc tatttgattc tccaatattt ccatcccttt ctctctatta
     1261 gcatcataca tgatttattt tacatgtcaa tatgcacatg taaaacgtgg aaactttact
     1321 gtgatataat aaagaaaata agacgagagg taggattttt atgaatactt tagcctatat
     1381 attattatgc gtgcttttat ccaaaccacg ttctgggtat gaattgaagc agctgatcac
     1441 aattttttgg gaagcccatc acagtcagat ctatacaacg cttgccaagt tagaccagca
     1501 gggttatgtg accgttttgg acaaagacga gcactctcaa aaaaagatct accacttgac
     1561 tgatgaagga cgtgctcttg tagaggaatg gatcaaagag gaaactcccg ctcctacgca
     1621 aaaagatgaa tttctagcaa aaatctatgc tttttcgacg ctcgataaaa caacagctac
     1681 tggactgcta ttcactagag aacaacagct gaacaaaatc atgcaaaaaa atcaaaaaaa
     1741 attgaatgac ctctcgctag acaagaaaca agaatttgga cgttatgttg tgatccagcg
     1801 cagaattatg ctttgtcagc aggaactcca atggtgtcag caagtaagag aacaaatgca
     1861 gcagtttttt gagtaaggga taaacagaac ctttctagaa atatctattg aagagcaaat
     1921 tatcttcaat aaaagagcaa tacagttgga aaagaaaaag cgatccgaac gacaggccaa
     1981 aacctatcgt tcggatcact atttttctag aactgcattc aagagactat gacaaaaagc
     2041 atgccaaggc cttttttcaa ataacagtgt tccatcattc attactttga attgctttta
     2101 gcacacttct tgaaagcggg ccaacgatga tcaactgcaa cggtaaagcg gcaatcacat
     2161 tcattttcca agcattcata taagcagaga ataattgatc aggtattcct tgttccatga
     2221 tcacaccgaa taaagacata catgtcacca ttcctaaaac catcaagcat gaaatagtta
     2281 agatcatttg gaccttgctt tctggattta ctggtagttt aaaggcaatt tttttcgcca
     2341 gcacaccgat gatgaagaca tccaaaatga aagccaccac gaatccaggc actagaccaa
     2401 tcaacaaact tgataaatga agatcttgat gcagccataa attatacgcg ctcatcccca
     2461 agaccatcaa aaaacacatc attgttgtaa aaattattcc ttctttttta tttgtaggca
     2521 atctgtacca ctcctttaaa tttgtcacga aaatcatcgt agcacattta aaaatttctc
     2581 gtaagtactt tttttatttt ctgccctttt atttcataaa cttcatcagc cgtacgctcg
     2641 acaaagtaag aatcatgaga agtaaacaaa atcgttcccg gatatatttg cattagcttc
     2701 tctaaagctt cgatcgttgc catatcaata aaattagtcg gttcatctaa cagcaacaca
     2761 ttacttggct tagtaaaaag caaagcaatc gtcagacggg tcgcttctcc cccacttaat
     2821 gtacataacg gtttcgtcaa agtttcctga gcaaacccta agtgattcag caaactacgg
     2881 acaagcgatt ctgaaaactc tgtttgtctc atgacaaaac gaatgatgga ttcttcagat
     2941 gtcatttgat aacccttttg ctgatatact tgaaagctta ctttcggaga gagaaggatt
     3001 ccttctcctt gtttgcggat atgttctaat aaggttgtct tacctgaacc attttcaccg
     3061 acaatcgcga tttttttacc taaagaaaac tgaaaatcac catttaccag taatgttctc
     3121 ccacttcttt ccaattggac attttgtccc atgatgggat agcggctgtg gagttcaaga
     3181 gattttggta ctggaaaacg gatttgtttc atctgctgcg gcttggttgt ttctcctatt
     3241 ttttggagtc ttctctctaa tgttttcgca ttcttttgga tggctttttg tacactgtct
     3301 ttttgtttag aggaagacaa ccgatccagt ctgatttgtt gctttttctt tttactggac
     3361 actttttcca acttttccgc ttgttttttc ttttcttgga tcgatttttc caatcgtttt
     3421 ttctctttca tgatcgactc tgcttctcgt agctgagtct ttttctccaa ttccttttga
     3481 gtgagatagg cactataatt ccccgaaaac tctcggattt ctccgtcttt tacttcccag
     3541 attttcgatg ctagctcatc tagaaaatag cgatcatgac taacaaaaac cagcgtacca
     3601 taataataac gaagctcttc aatcagtcga tcaatacttt ttctatctag atgggttgtt
     3661 ggctcgtcta gaagcatccc ttgttcataa gaagaaagaa gacgtatcaa ctcgatcttt
     3721 cgttcttccc ctccgctcca tttccgctct gaagtttctt ccagtcttaa ctggaaatga
     3781 ctggctaatt ccaaatttag gtcctttgct tcagcgagat aggttaattg aggcaggtag
     3841 ttgaaggtga tctctgtttg gattttccca tgatcgggaa aaagttcacc ggctattagt
     3901 ttgagcaaag tcgatttacc gacaccattt tctcctatga tccctatacg atcttgctga
     3961 tagacacgaa gagaatcaat agatagttct agctggttgc caaagctgac ttgtagattt
     4021 gttatattta ctgctaaatt ttccatatgt tttacactcc tatttcatgt aaaacaacaa
     4081 ttatcgatac taaaaaagag cagaaaaatc tgtttgcaaa aaaactgcat cacatctttt
     4141 cctactctga acgaaataac tatgactaaa tcaccaaatc tgtcatagct ttcacctatt
     4201 ctactgtcgt tcgaaaaaat ggacatactc catccatgaa attgttgttt atacatgtct
     4261 tttgaattta tttagttgtt attcaaaagt tcgaaacgta atttcatcga tgcagtcatt
     4321 tttccgacct ccttttttat taggttacca cacacgattc aaaaaaagca atagctactg
     4381 aatagtaact gaaaaccttt tattcgaaca tagtgatctt ttctggtgct aaacgaacag
     4441 actcgtaacc ttcttctacc gcttttccta ttgataaaat aataactgga acataacggt
     4501 ctttatccaa accaaatgcc tctgccaact gatctgcttc gaatccacca attggatttg
     4561 tttcatagcc atgatcacgc gcaactaaca tgaattgcat agccgctaag ctagaatcga
     4621 ctttgactac atcgttcatt tcttctcttg aaaaactctt gtaataagga atgatcgcag
     4681 ctaattgttg atctctgact tcttttggca tcttccctga ttcgtaagct tggttgtaga
     4741 tttcttcgcc atattcgtaa cattgcatat cgccaaaaat catgaccatt gcagaagaag
     4801 tatcattttg tctcgtattg aaacggatca aaggacgcaa gatttctttt gcttcctcgc
     4861 tttcagcgac cacaaagcgc catggtttca tattgacaga agaaggtgca gtcgtcgctt
     4921 ctttgatcat ttccagcatt tcttcgtgtg ggattttgta tgtttcatca tatacgcgaa
     4981 cggatcttct accagtcata attgttgaaa aatcattttt ttgttccatt attattaacc
     5041 tctttcgttt ctgttgttat attgcaaagt tgggtattca atttatctaa aagtgttaga
     5101 aattgtttca tttctacatc gctgaaagcc ggggatagca cggaacctgt atctttatgg
     5161 tccatttcac aatgcttgat tttcttctgg gctttttctg ttagcgctac atacacttcc
     5221 cggttgttct gaggatttct ctgacgcgaa acatagccat ttgcttccaa taccttcaga
     5281 tgacgagtaa ctgctccttg atcaatctgc aacttttctt gcaaactgct ttgtagcaat
     5341 ggattttctt ctttcagtct catcatgatc tcgtatctag taagacttat accgatattt
     5401 tcttcaaaga tctggacgat tttttgatcg gctaatttga tttgatataa aaggtgacta
     5461 atctcttcta aagacataat ttcctccttt ctaataattg actcgtcaac gattgacttg
     5521 tcaataatag aataaaaaaa gaaaaaagtc aaagaatcag ctgtgtaata aaaaaaacgc
     5581 tctcaataga aaagtataga gaaaagaaat tttttgtaga atgaacggaa aaaatgataa
     5641 agaaagcaca acgctaaaac caagattatt gattcttatc aaactataga agatagctct
     5701 tctgcagtag ataattttac accgccaaaa atttccgaaa tcgccttttg caagaatccc
     5761 gtcgaatatt agaaattgga tcagtatgct taaacagtaa taaaaaagtt atgacaagat
     5821 gttgtcacaa ccttttttga gtaaacggtg ttcaaaaact tgcatctgcg gtaataagtt
     5881 caaccaaaca aaaaacatga aaatctgttt ttgtttggct gttcttacta cttgatgcag
     5941 ggcagctttt tcactacctc tttttaataa ccataaaatc ctattccttc atatctccag
     6001 cccatcggtg ttagccaatc tctttcctta gcactcaagg tatagtgatg ggtaccagtt
     6061 ttttcatttg gattaaatag tcgataaatt ggcttggaac caccagaata aaaacttaga
     6121 ccttcataac gccaaccgat ttttgttaaa gagttgatct cagattgtgc cattgtgtaa
     6181 tgatggtctc cactgtaagg attgtataaa cggtagacag gttgtccgct gtttggtgct
     6241 tgccaagcaa tcccttcata ttcccagcct acagttggca gataattttt ttctccggca
     6301 ttcaatgtat ataaatgttc tcctgtatga cggttataca gacgatacat cggagaagtt
     6361 gaagttggtg ctgtatcata ctgtgtcaga ttataggttt cgattacatt gttcaatgta
     6421 gaaccataat taggcgcagt ggcataacga cctgttagcc aagccgtagc atctttgtaa
     6481 gagttcgtat tgcttttcca tgcaccggaa tagtagtaaa cgcctggctg aaaagaagtc
     6541 gtcttcaata ccgttacgtt atcttgcaat gattctttgt acgaaggata ttttctaaaa
     6601 gcggcattca ccgtaatcca ttgcccattc acatattccg aagtaggcat cgtaacagat
     6661 tggccattat agcttccttt aatcccaaat agattgtaat taggggcaga agccaaagta
     6721 ctgttccccc agccactttc aacgatggct tgagcaatca tgactgatgc atataagtca
     6781 ttggcacttg ccaaactttg tgcagttgag gcaatactat caatgaacgc ttgttgagaa
     6841 ttagcagatg agcgagcata aaaagcatag ccagtatagt tttcgaattc agacaaatct
     6901 gcttgatggg tatgtgtcaa tgtttgccca tctgtcaatt ggttcgtgat ttcttctgca
     6961 ttctctggat tgttctgagc ttctgctgat gtactgtctt cggtttgtgt ttgttctgtt
     7021 gtttcagttg ttggttcttc ggttgaaatt gtattttctg agacagtagc tgactgcaaa
     7081 gtactttctg tatcgttttc aattcctgtt tctgaagaac tcgatgtctg agttgcgaca
     7141 gtttctgtcg attcttcagc tagcactgtt gtgttggcaa ctgctagatt tcctaaaaga
     7201 ataacgctaa ataataaatt ctttgctttc atcttttttc tcctttgaat tatgaaactt
     7261 ttcctaaata acagtgtaac taaaaaagta aaagcaagtc aatgtccata tttaaataac
     7321 atttgtttct ttttaataaa tttgcttttt ctgcataaaa agacgtaaat gaagaatatt
     7381 cttctccatt cacgccttaa attgatggtc gtttgaataa ctatttaaca agtaattctc
     7441 tctggatatt atccgttatg gattatactt cctgtgtcgt cggacggata atcatgttgc
     7501 tgacagacat ccggtctggc gtttctatcg caaatgctac agcagaagct atatcttcag
     7561 atgttaatcc ccattctttt tgcgcttcat gaagttcaag agcagccttt ttgtcgctga
     7621 tggtttgata taattcagtt tgaacagctc ctggagagat gatcgtagat ttgatgttat
     7681 tttgccgttg ctcttgacgt aatccttcca tgattgcacg gacagcaaat tttgttccgc
     7741 aataaacggc ggaatcagga taaacaacat gtcccgccac agaatcagtt gaaatgatat
     7801 gcccagattt ttgtttttcc atgattggaa gtacggcgga aattccattt aacacaccca
     7861 tgatgttgat atcaagcata ttttgccatt catcacggtg accttcaact aatggagcgg
     7921 ttggcatgat tcctgcatta ttgtacaata catcgattcg accgaacttt tccatcgcta
     7981 aatcgataac tgcttggact tgtgcaaaat ctcttacatc tgctgtttga atatatagct
     8041 cagcttctgg taaagattct tttattgctt ttaggcgatc ttctcggcgt gcagctataa
     8101 ctagtttagc ccctttttct gctaatagct tcgttgttgc ttcaccgatt ccgcttgaag
     8161 cgcccatgat gacaatcact ttttctgata aatcattcat tctattctcc tcctgttttg
     8221 aaaaatctct tgcttatact cactatctct tatttcatct tactgttcaa ataatccgtt
     8281 cgacaggttt gacgattttg gcagggattc ctgcaacgat ggtattgtct gggacatctt
     8341 tcgtaacagt gcattcgctg caacaatggc attctcgcca atcgttacgc ctggcagaat
     8401 agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta ctgacaagcc
     8461 tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac gaggaccaat
     8521 caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt gacgattgat
     8581 aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag gcagcagaac
     8641 agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc ggatatctgc
     8701 tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttccg cttggatctg
     8761 atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga tttgtttttc
     8821 gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga cggcttatga
     8881 tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag tggtatgctt
     8941 ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt tagcagtagc
     9001 tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac ctactctctc
     9061 aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc gaggctcccg
     9121 agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag aaatcttgtc
     9181 gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg gagaaatcta
     9241 cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc attcgttgat
     9301 caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg atatcataga
     9361 caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa acaaacaaaa
     9421 ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac atcggaatca
     9481 tcctttagca aagcaaacag tcatccatcc gcaggaactg aaaactgttc ctttgatcat
     9541 ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca gcatagatca
     9601 gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg tcgataacgg
     9661 gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg aaaaagtatt
     9721 catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa aagaccagcc
     9781 tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca gttaagtaga
     9841 gtttttttcc ttcgctttca gctttttaac aacgaataag ttaagcaaaa gatccaatga
     9901 ttaaataaca taagtttctt aatttgttcc ttccttttat ttctgataat tccagtaaat
     9961 tactataatt ataaaaaaag aattgacaaa acataactgt ctatataaaa ttgattacgt
    10021 taacaataat tcgtttcatg tgactagatc atactaggca tgaaaaatct ctgaggaaca
    10081 tggtttccct aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa ctggtctagc
    10141 aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa gatctttaat
    10201 caaaatgcag tattggtaga tgacggtgga caggaaaaag tagctattgg aaaagggatt
    10261 ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg gatatttgtc
    10321 atggaaccag aaggtcaaat caagttgcaa aatttactta atcagattga tgaacgcttt
    10381 ttatttgcag cagaaacgat cattgatcat gcggaaactg ttttgatgga aaagttgaat
    10441 gaacatgtat tgatcgcttt gaccgaccac attgcttttt ctgcggagaa cattaataac
    10501 ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag tgctttacag tgatgaattc
    10561 agtattgctc aatgggcagt agagtatctg acaaaagagc ttgggattcc atatacttat
    10621 gacgaagcag gatatatcgc aatccatatt cacagcggac gaagcggtcg gaacaataac
    10681 catcgcagta tccgtgaagt gacgattgta tctgacatca tccatttagt ggaatcagaa
    10741 ttggaaacag atatccattc agaaccattt tcactgaatt attcaagact ggtgaatcat
    10801 ttacgactgc ttctacaacg aacccatgcg caacaatatg ctgttttaga cacggaaatc
    10861 gtagaaatgg tcaaacgaaa atacccagaa agctataaga tttctaaaaa aatccgtgtg
    10921 ttgctgacca aagaatacca gctcgcgatc acgaaagaag aactgggtta cttggccatc
    10981 catatcgagc gtttaagaag cgcaatcgta caaacaaatg ttaacaatca tgaggaggaa
    11041 aaaaattgaa agcatatcta caacgaatgg gacgttccct tcaacttcca gttgcggtct
    11101 tacctgctgc tgctttacta gtagggatcg gaaactggtg ggcatcttat agtaacgaca
    11161 ttgtcgctca ttttttacaa gctggcggta acgcagtttt aggtcaatta cctttgctgt
    11221 ttgccgttgg gttagcacta gggatgtcag tggacaaatc aggtgctgct gccttggcag
    11281 gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac tgattcagta gctactttat
    11341 tgaatatcaa accagaatca gttgatccat cttttgctca gatcagtaac gtatttattg
    11401 gtattattgc cggattgatt gcggcagctc tttataatcg tttccacgaa gcaaaattac
    11461 cgatggcttt atctttcttt agcggcaagc gtctagtgcc aatcttggct gcctttgtca
    11521 tgttgctgat cagtgcagtg ctactgctcg tatggcctcc ggtttactct gctttagttt
    11581 cttttgctaa attcattttg agtttaggct ggggtggtgc aggtctttat ggcttcttca
    11641 accgtttact gatccctaca ggattgcatc aagcaatgaa ctccgtgttc tggtttgacg
    11701 tagcaggaat caatgatatt ggtaaatttt tggcttctga aggaacaaaa ggcgtaacag
    11761 gaatgtatca agctggattt ttcccaatca tgatgttcgg gcttccagca ggagcatatg
    11821 ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac agcttccttg atgttagcag
    11881 cagcttttgc ttcattcttt actggtgtaa cagaaccact tgaattctca tttatgttcg
    11941 ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg gatttcttta gcagtgagtg
    12001 cattcttcca ttggacagca ggatttgcct ttagtgccgg atttgtcgat tacttcctat
    12061 ctttgaaaaa tccgattgcg aatcagccat tgatgttgat cgtccaagga ttagtaacag
    12121 cagtgattta ctactttggc tttaactttg ctatcaaaaa attccatctg atgactcctg
    12181 gccgcgagga agcagatctt tcagatgacg atactgcaac gactaattca gacaataaat
    12241 atgcggcaca agcaagcaaa atctacgctg cattaggcgg agccgacaat gtaacttcga
    12301 tcgataactg tactacacgc cttcgtttgc aagtcaaaga tacaggcctg atcgatcaaa
    12361 ataaaatcaa agccactggc gtacctggta tgaagatcat cgacggaaaa aatgcgcaag
    12421 tcatcgtagg tacagaagta caattcgttg cagatgagat ggcaaaacta catggtggtg
    12481 ctgcaacacg acctgcacaa acaaacaccg tggttaaaac agaaacattt gccggtggaa
    12541 cgatcgaaca agatatttac gctatcgcaa atgggaaact gattccaatc actgaagttt
    12601 cagatgatgt attcgcggaa aaaatgatgg gtgacggcta tgctgtatta ccagaaaatg
    12661 gagaaatctt ctctccaatc gctggtacga tcacaaacat tttcccaacg aaacatgctg
    12721 tcggaatcca aacagatgct ggtatcgaag tcttgcttca tatgggcatc aacactgttg
    12781 acttaaaagg cgaaccattc actttatatg taaaagaagg tcaaaaagtc gcaagaggtc
    12841 aattgattgc tttagttgat cttgccgcta ttcaatctgc aggcaaaaac acggatatgg
    12901 tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga aatcgaaccc gcacgtgatg
    12961 taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc ctgagccatc gtcttcccca
    13021 gactttttta tcgataaaca aacaggagct gagacaaaaa tcttgtctca gctccttttt
    13081 ccgaataaaa acggcgttct cctgccaact ccgcggtaat aagtccagcc aaacgtaaca
    13141 tatgaagagc tattttttat ttgactcttc ttattacttg atgcagaaca gcttttttac
    13201 aacctctgat aaacggtgtt caaaaaccag tttatcggta tgaagtcaaa tttatgcaaa
    13261 aatgtggaaa gccattttca caaaattttt cttcatactc aaaactaatc ggctttttca
    13321 caacctcttt atacattatt cctgatccaa tatttctttt aatgctgctt caagagaagt
    13381 taatggtttt cccaatactt tttctaaatc atcagaggaa atagctaact gatccttttt
    13441 aatatcatgt tggatcgcta aaaacatgtc agctgcttct tgagggaatc cggcttcttt
    13501 tagattttcg acaaacgctg catcacttgc ttcaataatt tctaactttt tacctgtcgc
    13561 acgctctaat gccttggcta attcttcata gtcagttaga ttgccggata attctaagat
    13621 ttctggagag tcactttcta ctagtacttt tgctgcagct tctgcatatt cacgtttcaa
    13681 tgcccaacct gtttttccat ttcctgcagc ataaacgaat tgaccagttt ttaaagcctg
    13741 cccaatgata ggtaattcat tctctacata ccaattattc cgtaaaaatg tatgggaaat
    13801 acctgaatcc ttaatcattt tttcagtgaa ttggtgatct ggcgctaata cactgatcga
    13861 gtgatcggca tctgcaaaac ttgtataagc gatataagat accccagctg cttgtgctgc
    13921 cttcactaca ttcttatgtt cggcttgacg atttcctgga acgccagaga caaaaagcaa
    13981 tcgatctatt cctttcaacg cttctttcat tgagtctaaa tcatcataat caccaatacg
    14041 tatattaaat ccagcttctt tcagtgcttc tcctttttct tcactacgta ctagtacata
    14101 gatttcattt tgtggaacca tcttttttaa gaattccagc gcataaccac caaaacctcc
    14161 agttgctcct gttactaaat aatgcatatt tgccactccc tattcttttt atttgttaag
    14221 atttacagct gtacatttat aaattacacc tttatcataa agagtgtggt aagataagtc
    14281 aagcaaaaaa aatgaaagga tgacaactga tgaagtattc tctgcaattt agtgatgcaa
    14341 tccatgtact tgcctatatt gaaatgtttc aagatacccg atcactttct agtgagatga
    14401 ttgccagtag tatcgaaaca aatgcagcaa atgtacgtaa aatcatgagt cagctcaaaa
    14461 aatccggttt gatctttaca aaaaccggaa aaccgcagcc ttcccttgcc aaaagtccag
    14521 atcagatttc tttactagat gtctatcgca gcatcgaagg taatacacag cttatccaag
    14581 tcgatccaaa aacaaatcca aattgcgtcg ttggtgcgaa tatccaagaa gctttagaag
    14641 aaagctacgc tcggctgcag aaaaaggcag aagaagaaat ggctaagatc acacttgata
    14701 gtctgataca taaaatagcc gttttggaaa aagaaaaaag accagaaaac acaggcctcg
    14761 tagagagatt tttataattc tctctactgt gcctgcattt ttctgatcgg aatcgtctac
    14821 ataaattctt ttattctccg ggggacaaaa gccttgtcat gccccttttt cctctctaat
    14881 gctcaggtca aagcgctgaa gtcatgacgt cttgataaac ggtgttcaag tagctgaccc
    14941 tcggcattag ttctgaaaaa tgcaaaatat ggaaagctat ttcccatttt tcctctctaa
    15001 tgctcaggtc aaagcgctac tttcacgacc tctatattat catggtttat tgactaccgg
    15061 tatccagatt tctcccatat atttcccgtt ggtaactccc atttcgacta cagcatttgg
    15121 acccccaaca taggcgtaat cttctacttc tcttaaaaca tgcccaaagg ccgtattcgt
    15181 caaatcattt cttaattctt caggcgattc tgctaaagcg attacaacaa gatacgttcc
    15241 ttttgggaat gggatgattc gggtagtgtt tggaacatcc tgactcgttt gaacaccgac
    15301 atagtacatc attttcccgt tatagggttc gttgactgta aagaaataat gattttcagc
    15361 agcttgtttc aatgcatcta ttgttccatt accgataact ttttgctcaa atgcttgttt
    15421 ctctgcagcc aaaccgacaa aatccgtatt gctactcttc aattccacac caatacccgt
    15481 caccgtaaaa tctggttttt cttcgatcct atactttgcc atttctccca ccctttctat
    15541 tatattaagt tcatcttaca taaaattaaa gggaaatgac acgattacgc tcttttttct
    15601 agggatattt ttatttttta actatttttt tgctttcact gcaatccaaa tctcactgta
    15661 aacattactg aaatcctctc cccattttgt aaaagagatc tcgggagctt cgactaactc
    15721 ataatctgaa gaaggaagcc attctgcata gattttcccc caagtatctt gtaaagtttg
    15781 gggaaatggc cctttattag gaaaaatggc ccaagtgtgt tcttctatag caagcatttc
    15841 taaatcagcg tacggatttt cttttgtcgt agcaaagcca atcatgtgca tcaaactgcc
    15901 cttttcttcc aagcgggctc catcaaatcc ataagaaacg ttgattactt gatttggata
    15961 taaatctcct aacttatgca tctcttccct ttgagtctcc gtgatcgtct gcgctaactt
    16021 ttgtatttct tgattgactc cttcaaattt gacaggaact tttttcgcca cacctgccac
    16081 ataaaactgt tctttctttt cgatacgaaa ttccatcgat tttcctcctc tgacatcaat
    16141 aaaaaatgtc aagcgtggaa aagatttttg tatgccgtat ctgctgattt ctgaaggcag
    16201 atatcctgac caatcacgaa aagctcttga aaaaccttcg actgtttcat atccatatct
    16261 aaacgccgtt tctgtcactc ttgatccatc taggagatcg cgattagcca aagccaatct
    16321 acggtttttg atatagtcca ttagcgacat ccctgcaata aacgtaaaga tgcgttttaa
    16381 ttgatattca gagattcctg tattttcggc tattatttta taggaaagtc cttctgtcaa
    16441 atgttcttcg atgtaatcga tcaattggtt cagatcttta agcatattta tcttcctcct
    16501 tgcttgcctt tttatcatat tatcaaagaa agtcaattac ttgatgaatc aggaaccaat
    16561 ttagtcagat gtagaaaatg ttgtatggat ctaaagaaaa ttctcaagca gcgtttcgta
    16621 acggataatt ggtattatct gcctatctat gttttacctt tagaaaaata aattttgggg
    16681 aagacacatt ctaagtaaac aaagcggtaa aaatatcttt tattccctta gttaatccgc
    16741 ttactaccta ctgtacaaag caaattgttg ttcaaactgc tgatcagaac gctgcttgga
    16801 tttttctttg cgatacatca tacctctttt atcaattgga acaattattt atttctaaca
    16861 aaatcacttc atttttgtca gataaataaa aaacaaaaaa agttccatct ttaaaacacg
    16921 ctattctttt actttttctc acattctcta agaaaagaaa aggagaatga atttttgaca
    16981 attcgagcaa aagtgattat caaatatgta aaatcagcta cttttttctt aaacgtttct
    17041 ttatactaaa tataaataag tgtttcatga aaggagaact agatgaataa ttggcttgag
    17101 tattttcctg aaaacgtttt ggaacgcggt tacagctatc atctgcacgg ttttgtccgt
    17161 catttgaact atacaagcaa gtacttgtcc gcaacagttt ctggcacgga agattataag
    17221 gtagtgatca cttgggatga aaagaccaac atgacttgtg attgtctata tgccatagaa
    17281 ggaaaaaaat gcaaacacat ggctgctgtc ttattcgctt acgaagaacg tccaataaaa
    17341 aaatccaact actcactttc cgaactctcg tcccttgttt cctccgctag ttcctctctt
    17401 gtacgggagc ttttaacaga gattcttata gaacatccgc aatttatcga acgcttcaaa
    17461 gttaagatgc catttcatgc aataaattat tcagataaat taacaactat catccataaa
    17521 tacgatcata ttatcaagaa aaacaaaaac aggaaaacag cgaagttcat catggagatg
    17581 cgaaagttca tccaagaagc agttgaatcg ctcattcagc aaaatgccta tcttcccgca
    17641 ttcgaattga tcaatgaagt gattgctact ctcgaaacat tctattggga gcctgaggat
    17701 gaacgaaccc ttcttctgat agaggactgt tattatttat ggaaagaatt attagcagaa
    17761 gcacctcatg cagaaaaaag gcaaatgttc tcttggttcg tctgccaagt cgatcatact
    17821 gacgcctctt attcaaaacg ctactccata aaaatcctga aagaagactt tcgggaaaaa
    17881 gaattttcta accaaaagaa aaaaatagat aaaaaaacta aagaaacggt aaagaaagac
    17941 gatttcaatg aaaaatagca agtaaacgat aacaaccatc ctaaatccac aagaaataca
    18001 gcagaccttg ctttaaaaac acgaatcatg caaaggatga agtaaagaag acttaacgcg
    18061 tacccaatat tagtcatttg actattaggt acgcgttttg ttttataagt catgacaaat
    18121 ttccttcgtt gctgggtaga ttttccggca ttgttgatag attttatgat acttttctac
    18181 attttgtaag agaggttcca ctgcatctgt atattggata aaattctgac aacaactttc
    18241 aggtgtgtcg aaccactcta atctttaaag ataaatatta aaataacgat taataatatt
    18301 aacagtcttt atttttcacc accaaaaacc tatctttgca tatactaaat agacgtatat
    18361 acacagatag gtttgtcttg tgaaaaataa tgattatcct taccaaaaaa tgtttgtttt
    18421 tctacatgtt cgtacttatc aaaaaatatg atccttattg aaattactgg ctatttatat
    18481 tccaacgact tcttgtgtta atttttgtta tctcatagta aattatgttt cattttcact
    18541 taatttagtt gtcacgtgtt cgtcaattcg taaaaacggt aaatatataa acacacttgc
    18601 ggctataatt aatatttgta aaacagctgc tcgccagtct ccggctgttg ctaaaaatcc
    18661 cgatataact ggtggcgtag tccaaggaat ttgcactact acatgattaa ttaatcccac
    18721 agcagttgca aaatatgcta ctgtcaaaga aaaaatcggt gcaagcacaa agggaataat
    18781 cagactgata ttaaatacaa taggcaatcc aaaaataata ggttcgctta tattgaatat
    18841 agcgggagtt accgataatt tagcaaagtc cttataatct tttctacgac tgaagataaa
    18901 tattgcaatc aataaagcaa tcgtatttcc cgatccaccc attaccgcaa aggtatcttt
    18961 aaaggccata ttgataatat gtggaatttg ctctttgttt gcataagctg ccatattttc
    19021 ttgcatattc tgaatcaaaa acggatctaa caatgaacct gatattactg cttgatggat
    19081 accaaatcca aaaaatagat tagcaataga tgtaattaac aaaaatcctg gtaaaccagt
    19141 tgtaatttta cttaatggct ctgtaactat cgtggtaatt agcttattta tatccatatt
    19201 aaacaattga atgattataa atgacaataa agcaaacgag gtaatcgtaa taatgattgg
    19261 gattaataca ttaaaagact ttaaaacagc gggaggaata tttccggata gctgaatctg
    19321 tagtttctta ttattagaaa gtttaataaa taattctgta gccgctaatc caacaaatat
    19381 acctacaaac attcctgaag ctcctgtata ggatataggt atcacacccg tcacttcaac
    19441 agctttatcc gcattttcag gagtaaacat catctgcata ggtgttaata caacaaatgt
    19501 cgaaatagca actaataccg ctgcaatatg atttgtataa tttctgcttg cacacatatg
    19561 ataagatact gctgctacaa ctaataatgt agttatgctt aatgtaccat tggcaattga
    19621 agtaccaatt tgttgtaacg tggtcaatgt actagtatcg acaatttccc ccatgaatcc
    19681 tgttggttct aagatcactg aattaataag tgtcacaaat cctgctaaca tcataaatgg
    19741 cataaatgta gcaaacgcat ctcgcaaagt tcttaaatgg atctgattcc ccaacttagt
    19801 cgcaactata ccaaattttt caaatacaat gtccttcctg ctacttttgc ccatattttt
    19861 tctcctttat gcttattgta atcgattact ttttccaccc aaataataca tcacttatca
    19921 aaaaaagtaa atacttttaa ttaaatatat atatcttttt tagtataaaa aaataagctc
    19981 tgaaaaaata attttcccga gcttattttt caacaaaaac agcttcaaaa acaaagtctt
    20041 gatataaatg tctcgatacc gaatactcga aaggtttacc agacgataga gcaacaactt
    20101 tgtctatctc aatcataaat tcacctggat ctatttttaa tatttttgcg tcaaattcat
    20161 tcgcagtatc tcctttgata cgtatgtgcg aagtctttat ttccagaccc agttcatttg
    20221 tgatataagc atatacagat ttctttaaat gttttggttc caagcctggt atgactgcta
    20281 atggcatgaa tgtttgctca atcgagtatg gttgacaatc aataaaacgg cttcgtacaa
    20341 tattatatac atagtctttt acactgatac ttaatttatc agctactttt ttcgatggaa
    20401 gttcaattga gaaagtatgg atttctgaat ctacgtgttc ttcaccaacc acgctaaaca
    20461 aaccttcgtt tggaccaaga actcgtgagt tatcaattgg tgttctgacg acttcagtcc
    20521 catacccact tctacttcgc aaaactcctt cggaaacaag taagtctacg cctttcttta
    20581 ctgttaactt gcttgtatga taaataactg ccaattcatc tcctgtagga agtttctcac
    20641 caattttata ctctccattt acaatttttt tctttatatc taagtaaatt tccatatact
    20701 tcgcaatttt tttcaatggt gaccctccaa tcaaaaacat atcttcatct atactttaac
    20761 ataaataaac cgataaaatc cacatcaaaa ttatacacac aataaaaaag atatatatat
    20821 ttaaacaaaa ctatttactt taagttgtaa gcggtgttat cattaagtgg aaacaggagg
    20881 taaaaaaatt atgacaacta tgaaaaagct aacttttcca gatgattttt ggtggggttc
    20941 cgcttggtca gctgagcaag cagaaggaaa aggagaaaca ggaaaagcag agactatctg
    21001 ggatcgctgg tttaaagaac aaccttaccg gttttatcaa cgaatcgatt cctctattac
    21061 aaccgatcac atccatcaat ataaagaaga tatagaacta atgaaaaaaa ctgggcataa
    21121 ctcctttcgc gtttctattt cttgggcacg aatgtttcca aatggcggta ccggaaaagt
    21181 aaatcccaaa gccatttctt tctatcgaaa attattttcc gaaatgaata ataacggaat
    21241 caaagtattt gccaacttat atcattttga catgcctgct actcttcaag ataaaggagg
    21301 atgggaatca cgtgaagtag ttgatgctta tgttcattac gctgaaactt gttttaaaga
    21361 atttggtgac ttagtttgtc attggtttac cttcaatgaa ccacttgggc ctattttagg
    21421 tacttatctt gaagattttc attatccaaa cctcattgac tttaaacgcg gagcgcaagc
    21481 agcattcttc actatacttg cacacgcaaa agcaatacaa gtatttaaag aactagctct
    21541 tcctagtaaa ataggcgtta ttctaaattt gagtcctact tttcctcgta gtcaaaatcc
    21601 tgctgatgtg aaagcagcag aaatcgctga tttattttat acgcggagct ttttagatcc
    21661 tatggttaaa gggaattttt ctaaaaagct tatcctgtta ttacaagaat ataatcagct
    21721 tcctgaagat tatacgaaag aagatcttgt atgtatctac gaaaataccg ctcaaattct
    21781 tggactaaac tattacgagc cacgtcgcgt caaatctcgt tcttctatgg tggatacatc
    21841 tagtccattt ttaccagagt ggtttttcga accacatatc atgcctggac gacgaatgaa
    21901 cgaataccgg ggatgggaaa tttacgaaaa aggaatttat gatttgtgca tggatatcaa
    21961 gaaccattat ggaaacatcg aagcatttat ctcggagaac ggaatgggcg ttgcggatga
    22021 agaacgtttc atggatgata cagggcaaat aattgatgat tatcggattg agtatatcaa
    22081 agatcatctt gcttacttat ggaaggcaat aaatgaagga tgtaacatta aggggtatca
    22141 tctttggaca tttattgatt gctggtcatg gatcaacgcc tataaaaacc gatatgggct
    22201 ggtttcactc gaccttacta ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa
    22261 gcaattaagt gatacaaatg gatttagata cgatgtagac ttgttagttg aataatattc
    22321 taaaattcca cttgaagtga acacattaga aaacaagaat aatgagaaat accaataaca
    22381 aatacttttt cttcatactt catttatact tgtcagtata agagactgtg acaaatgtag
    22441 tgatcctcag aagttaggtc tggaaatcta acttgagaag atttttttaa actacgtcca
    22501 gtctttttta tttgtttaaa attgaagttg acaagtcttc attaaaaatt tagaatattc
    22561 acaagtttga cgacaattat gaaaatttat gtcgtgctaa aggagttttg ttattggatg
    22621 aaaagttaga tataaaaact gcgatcaaag acacgttacc taccgttttt ggttatatcg
    22681 gtattggact tgcatttggt atcgttggga aagctgccgg gtttcatccg ctggtcgtca
    22741 cgttgatgtc cctgctgatc tatgctggtt ctgcccaatt tatcacggtc agcatgcttg
    22801 ctagccatag tccactgctc tccatcgttc tctcgacctt tctagtcaat tcccgaatga
    22861 ttctgatgag tatgacgatt gctccttatt tcaagaaaaa tcggctgctt caaaatctgc
    22921 tgattggcac attgctgaca gatgaaagtt tcgcactggg aatgaataaa ttgaactata
    22981 ctggacaaaa gttaaacttt cgttggatga atacagctaa ttggatttct tatttgacgt
    23041 gggtgggttc ttctcttgtt ggcgcacttt tagggaattt tattactgat ccgaaaaagt
    23101 ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt actttatctt caagtcattt
    23161 ctgatagaaa cacctctaaa cgattacagt tgatcctgat tggtctcact ttgattctgg
    23221 tatatgtcgg actgattttt atcccaagta acttagtgat tttagttgta acactgattg
    23281 ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg agtttacatt gctgactatt
    23341 ttaggttgta ctttagcaac ttggctttct aggatcctac catttgtctt actgaaaaaa
    23401 ttcgatttgc cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt cattatgtct
    23461 gctttatggt tcagcagtct atttacccaa aatatcggac atctgccgca gatcaatctc
    23521 gaaaatgctt tggcttcagt tcctacactt ttagctgcta ttctttcaaa aagcttattg
    23581 gtgatcgtct tggtaggaat tctttccctt tcgttgattc gcttattata aataaaaaaa
    23641 caagagctta tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa
    23701 aaaatcggcg tattcttgct tatccgttga acggaccact aacaacgact tttcctaata
    23761 tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt cttcgcattc atcccatctg
    23821 aatagacctt gctcaatgta ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg
    23881 ctaaagcttc tccttgcgtt tcgatttcat agttatagtc tgttttagcg aacatatact
    23941 cccagtcgaa gctgatagat tggtttttcc aaacacctat atctaacggc tctttgatcc
    24001 ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc
    24061 gggtaatatc gaataataca gcgacatact ctacttgatc gattcctaat tcacgaaaac
    24121 ttttacttaa gtcgtgatga taatcgattg gatagtctac acctgttttt ttcagccatt
    24181 caaaattctt cggacttgca gtcgcgtaga cttctaggcc agcccaatgc gctagttgat
    24241 tcaaaatcga accgacgccc ccagctccgt tgatcactaa gatttttttc ccgcggttgg
    24301 cattttcttc tggagtcaga ccaaattttt caaataacaa ttcataagcg gtcaatgagg
    24361 tcaacggcaa ggctgcggct tcttcatctg aaagattttt tggtgcaaga gcgacaatac
    24421 gttcgtcaac cagttggtat tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa
    24481 aaacgcgatc gccgacagaa aatttttgca cttcatcacc tacagcaaca actttcccga
    24541 ctccatcaaa ccctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg
    24601 tatcgacagg attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta
    24661 caggtatctg ttctttttca tcaataaagc tttttggatc atcgatcgac aacccttcat
    24721 aaaaaccgac taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc
    24781 aagttggagc atccatacag ttttcatttc aatatatata gtatgctatt tgttccactt
    24841 ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt tggttaaaaa
    24901 agaggttgcg acaagacttt tgtcacaacc tcttttaaac ggtgttcaat caactgaccc
    24961 tcggtattcg ctcaaaaaac gacaaaatat gaggagctat ttttgtttgg ctgttcttat
    25021 tacttgatgc aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca
    25081 ttgttcactg aaaagcgttg gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc
    25141 atcagtagat ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt
    25201 gatatagatt ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata
    25261 atgatcttgc agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc
    25321 cctcggttct ttttcaaatg cataaggatt cttgaaaatc ccccggccaa tcatgatgcc
    25381 atctacgcca tatttttcgg ccaattcttc tcctgtctgg cgatctggga tatctccgtt
    25441 gatcgtgatc agtgtttgcg gagcgatttg gtcacgtaaa gcaatgattt tagggatgac
    25501 ttcccaatgt gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt
    25561 ggctatatct tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata
    25621 gccgatacgc gtcttgacgc tgacaggcaa accgccagct ttagccgctt caatcaattg
    25681 tgccgctact tctgggcgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg
    25741 gactgggcag cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct
    25801 catttctcta aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc
    25861 ttctgtaaag gtcaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc
    25921 tgagtttgta aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg
    25981 aaatacaaca tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc
    26041 tttccaaaaa ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc
    26101 aatctttctc aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag
    26161 cttaaaagat gtttctgatt gagcaaaaaa agactgtgac aaaaatctta tcacaattcc
    26221 ttttccggaa taaatggtgt tcaaaagctt gcatctgcgg taataag
//
LOCUS       DACEDO010000040        26238 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEDO010000040 DACEDO010000000
VERSION     DACEDO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.040"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1199)
                     /locus_tag="IWO52_001685"
     CDS             complement(<1..1199)
                     /locus_tag="IWO52_001685"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0355954.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIV"
     gene            complement(1784..2491)
                     /locus_tag="IWO52_001686"
     CDS             complement(1784..2491)
                     /locus_tag="IWO52_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355955.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(2556..3392)
                     /locus_tag="IWO52_001687"
     CDS             complement(2556..3392)
                     /locus_tag="IWO52_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0355956.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3410..4819)
                     /locus_tag="IWO52_001688"
     CDS             complement(3410..4819)
                     /locus_tag="IWO52_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0355957.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4833..6656)
                     /locus_tag="IWO52_001689"
     CDS             complement(4833..6656)
                     /locus_tag="IWO52_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0355958.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7499..7825)
                     /locus_tag="IWO52_001690"
     CDS             complement(7499..7825)
                     /locus_tag="IWO52_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355959.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7871..8233)
                     /locus_tag="IWO52_001691"
     CDS             complement(7871..8233)
                     /locus_tag="IWO52_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0355960.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8270..8485)
                     /locus_tag="IWO52_001692"
     CDS             complement(8270..8485)
                     /locus_tag="IWO52_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0355961.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            8612..8947
                     /locus_tag="IWO52_001693"
     CDS             8612..8947
                     /locus_tag="IWO52_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0355962.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9023..11020)
                     /locus_tag="IWO52_001694"
     CDS             complement(9023..11020)
                     /locus_tag="IWO52_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355963.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(11118..11393)
                     /locus_tag="IWO52_001695"
     CDS             complement(11118..11393)
                     /locus_tag="IWO52_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0355964.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11625..12359
                     /locus_tag="IWO52_001696"
     CDS             11625..12359
                     /locus_tag="IWO52_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0355965.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12398..13540
                     /locus_tag="IWO52_001697"
     CDS             12398..13540
                     /locus_tag="IWO52_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0355966.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13635..15026)
                     /gene="sufB"
                     /locus_tag="IWO52_001698"
     CDS             complement(13635..15026)
                     /gene="sufB"
                     /locus_tag="IWO52_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0355967.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15031..15501)
                     /locus_tag="IWO52_001699"
     CDS             complement(15031..15501)
                     /locus_tag="IWO52_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0355968.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(15488..16723)
                     /locus_tag="IWO52_001700"
     CDS             complement(15488..16723)
                     /locus_tag="IWO52_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0355969.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16720..18006)
                     /gene="sufD"
                     /locus_tag="IWO52_001701"
     CDS             complement(16720..18006)
                     /gene="sufD"
                     /locus_tag="IWO52_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0355970.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18019..18792)
                     /gene="sufC"
                     /locus_tag="IWO52_001702"
     CDS             complement(18019..18792)
                     /gene="sufC"
                     /locus_tag="IWO52_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0355971.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18989..19837)
                     /locus_tag="IWO52_001703"
     CDS             complement(18989..19837)
                     /locus_tag="IWO52_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0355972.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(19861..20547)
                     /locus_tag="IWO52_001704"
     CDS             complement(19861..20547)
                     /locus_tag="IWO52_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0355973.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(20540..21574)
                     /locus_tag="IWO52_001705"
     CDS             complement(20540..21574)
                     /locus_tag="IWO52_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0355974.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(21969..22307)
                     /locus_tag="IWO52_001706"
     CDS             complement(21969..22307)
                     /locus_tag="IWO52_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0355975.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22309..22665)
                     /locus_tag="IWO52_001707"
     CDS             complement(22309..22665)
                     /locus_tag="IWO52_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0355976.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(22671..23858)
                     /locus_tag="IWO52_001708"
     CDS             complement(22671..23858)
                     /locus_tag="IWO52_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0355977.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24049..24894)
                     /locus_tag="IWO52_001709"
     CDS             complement(24049..24894)
                     /locus_tag="IWO52_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0355978.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25128..26093
                     /locus_tag="IWO52_001710"
     CDS             25128..26093
                     /locus_tag="IWO52_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0355979.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7672 a         5516 c         4455 g         8595 t
ORIGIN      
        1 acaatcaccg tcgttggaat cgatgtccca aagaacaagt tcgcaggcat accgatcaca
       61 gcataaatac tgccatcttc tagtaatttt tgacggatga ccccttctgc agcgccacgg
      121 aataaaactc cgtgtggtaa gacgatggcc atggtacctg tttccttcaa atgatagaac
      181 ccatgaagaa gaaacgcaaa atctgctttg gattttggcg ctaatttccc ataacgatta
      241 aaccgagaat catctaagaa agtcgtatct gctgaccaat tagccgaata aggtgggttc
      301 attactactg catcaaatgt gtaaggttca tctgttggcc agtccttatt caacgtatcc
      361 ccattgcgga gattcatttc ttcggcgtcg acaccatgta aaatcaagtt catttttgct
      421 agattgtatg tcgttgtatt tagttcttgc ccatgatatt taacgttatc tgggtgtgtt
      481 aagtagttac gtacgtttaa catcaatgaa cctgaaccca tcgttggatc aaaaacacta
      541 aagaatcgtc gttccttttg atcgagcgtt acgatttgcg ccatcatatc agaaaccata
      601 tgaggtgtgt aaaattctcc ggctttcttc cctgcttctg aagcaaattg actgatcaga
      661 aactcatacg catccccgat cacgtcccca tcgtgttcta aaacatctac atcattcaat
      721 ttcttgatga cttcggtaat cgtcacattt cgttgttgct catctgttcc tagttttttt
      781 gattggagat ccacatcatc aaataaccca ttaaattgat tgtaggtaga tgccaattgc
      841 acaaaggctt tgtttagatc gttcaattgg aacgttgctt gttttgcttg atccgctaag
      901 acgttgaaaa gatactctgg tgcaattgca tatcccaaaa tatcaacgat tgtggcaatc
      961 agatcttctt ttgattcttc atctgaaagt aattcacgat acaacattgt ttgtttgctt
     1021 actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaatcga
     1081 tcggataaat atttatagaa aatcagtccc aataaataat ttttatattc tgacgcatcc
     1141 attttactac ggagattatc tgccgcacta aataattttt gatttaattc tgcacccaaa
     1201 aagatacact cctatctctt atttaaagtt gctatatacc ctcttatttt agcatacccc
     1261 cttttataaa aagcaaaaaa aaaaagaaag aaacttattc ttgctaataa actacgcgaa
     1321 aggagtctaa cgttgtttga tataacttag actgatcgtt aacttatttt ttatatttct
     1381 acatagttat ctttagaacc atttcagata catatgaaac aattacatat gattttttca
     1441 taaaaaaaca gtactgtcta gtagtataaa atatacaaat ttcttaaaaa ttttttcact
     1501 aaaatcacta cctgaaacag tctaaaacac ttatatatca acgtttaatg aaactatctc
     1561 cgtttcattt cagatacaaa taaatatagt cgggctccaa atccgaaaaa aaataccccc
     1621 agtagcttat cctaagctac tggagatgta taagtacggt catataacaa cggggtaggg
     1681 taaataaatg aaaaagatgg aagaatcatt attcaataac ttttttaaat acttcgttgt
     1741 aatcagttac ctgtccgttc ttaaatacca cagaataatc tttttaccca ttttcaagta
     1801 accgaagtgg aatgtcttta ccaacttgct ctgtcactgc attactgata cctgttagat
     1861 tagctaataa atctgataaa gattgagcat ctgtagaaat ctctgtatca gtatttatcg
     1921 tgattccttt atcttcattt acaagcttcc aagtaggtgg tatagctgtg tatgtttcga
     1981 tgaatgaatc aattctttca tctatcgtct tcccagtatc tacttctgtc tcattactct
     2041 tagatgaatc gattctttca tccatcgtct gctccgtaac tgcttctgtc tcattattct
     2101 caccggaaac tctcttcaca cccaaactta tatattgtcc atctttagtg tgcatagtta
     2161 aaattcccac gcttttacta ataaattcac tataaccagt tctttcaata gagacttcaa
     2221 tgtatttcac actatcatac tcaaaagaaa aatagacatg taaaagcatt ttgttgttta
     2281 catctacata ataatccttt gactctactt gagaccttcc tatacggaat acagctttac
     2341 tgtctgatat ctctatacca gcatccttat tattaatctg ccaatctcca agtagctctt
     2401 ctggcaagct agtagtttct tgtgaacact ttaccacttc actggtagag gtcgaaacat
     2461 tcacctccgc actgttagaa gaattttcca aggaatcagg agccttagca caaccaacaa
     2521 gtctttatat attgtgcagc cgatattttc ttcattcagt acacccttga aatatgcagt
     2581 gttagataaa gcagttcgtc ctgatttgct gaggcaccca tctcttcttg aacgtactcc
     2641 ttgattttca aggccgcccg atactctttc ggaaaacgct gcttcactga agccagcaaa
     2701 aaatcatcct tctccggcaa ctcttccttc ttaatcattc gggcaataaa aaactgtaaa
     2761 tgcgtcacaa atcttaagta aagcatcgaa tctttatcaa cctttttttc gggaaacaga
     2821 tatcctagca tagccaagat atcctttagt gctcttaccg agagcatggt ttcttccata
     2881 tttgcttgtt cactttgggc atttacaatg tgaaacgcaa tgtttccagc ctcctcttct
     2941 ggcaactgaa cattcatttt tttgttgatc aattggatag catagcagcc gatctcaaat
     3001 tcttttgggt aatacttttg aatttcaatc agcaggcgat tttgaaaaac aatgcctttt
     3061 tgactgcgtt caatggcatg cttcaagtga tcaaccaagg taaaaaatag acttttattc
     3121 agcttaaagt caaacttggc gttggcataa tcgataattc gcttgacgca aataattaga
     3181 tcatccggag cagcctcaaa cagtttaatg taatcctttt ttagagcatc agaaagtgaa
     3241 tctaatttaa atactttttg gattttgtca gccggaacca aatcgccttt ctttagttga
     3301 aatccaatcc ctttccccat caaaattact tctttgcctt gcttatctaa gcaaagaatt
     3361 aaactgttgt ttaacgtttt aattactctc attagattca ccgttctctc taagctaagc
     3421 catttcttgc gattactttt tgataccaaa agaagctttc tttttttatt cgttttaatt
     3481 ctttaagatt ctcatcggtt cggtcaacat atataaaacc ataacgctta ttgaagcctt
     3541 gatgggtact aacaacatca atcgctgacc aaggtaaata accaaaaaca tctacaccat
     3601 ctgttaaagc tgcttgaata gccagaatat gtttggctag ataatcaatt cgatcctggt
     3661 cattaattcg tctatcttcc tcaactatgt cgggtgcccc atagccattt tcagtaatta
     3721 aaataggtaa atcatagcga tcgtacaatt tcctcaatgt taatcgtagt ccagtagggt
     3781 caactaccca gccatatggc gttttatcta cataaggatt ttcagctgcc cggtagacac
     3841 cttcttctcc caacatgatc tgttgatccc ccgctctagc ggaaacatct gatccgtcat
     3901 ttttgctggc agcgatggtg gcagttgaat aataattaat cgcaataaaa tcgggtttac
     3961 cggaggataa aattgcctga tcctctgcct caattttagg atataatcca cgatcattca
     4021 aatagttttc aaacaaataa ttgtattttc cccgaactgc tacatctaag aaagaccagc
     4081 ctctcaatgt ttcccaatta tgggctgcaa ttgcatcttc tggactggag gtagcttgat
     4141 acatagccgt taaattgatt gctggaccga tttttccttt aagattcaac tgatgataca
     4201 agtgaattac tctagcttga gctaataaaa catggtgatt aatttgaaag agttcttttt
     4261 ttgaaggcag ctctccactt ggtggcaaac caatcgctcc aggatgaagg atcatcgtgt
     4321 tttgctcatt aattgtcagc caatagttca ccttgtcacc atactcagta agcaatacct
     4381 gtacatagtc cacaaaataa tcgatagttg ctcgatttaa ccaaccgccc tgctcctcta
     4441 gaaaataagg taaatcgaaa tggtacatgg tgacaatcgg ttcgattccc tgattcaggc
     4501 attcatcaat tacttgatga taaaaagaaa ttcccttttg attggtttta ttattttcaa
     4561 tatccataat tcgcgtccaa gcgatggaaa agcgaaaagc ttttaagcct aattctttaa
     4621 acagctgtat atcttcttta tagtgatggt aaaaatccga ggcaacttca aaattagcta
     4681 ccccttctgt atgctgatac ttgtcaataa tagacggttg tttcccatcc tcctgatagg
     4741 caccctctgc ctggtaggca ctaatggagg caccccataa aaacttctct ggaaatgctg
     4801 aaagctgttt gtattgcaca aaaatcctct ccttaactaa tttgcatcaa tggttcgtgt
     4861 tcctttaagc ttccgcttgc ttcactaatt cctgcgtaat tttcactgtt ggtcactacc
     4921 acaatcactg tatcatcgta gccagcgttt ctaatggcag cgaaatcggc gttgattaat
     4981 aattgtcctg ctgacacctg atctcctgct tttacaaata cttcaaaggg ttcccctttt
     5041 aaattcacgg tgtcgatccc cacatggatc aataattcaa taccttcttt agaacgtaga
     5101 ccaatcgcat gtttagattc taaaatagta atgacttctc cctcaacagg tgaataaatg
     5161 ctgttttctt ctggatgaat ggctacgccc tttcccaata gtcccttaga aaagacagca
     5221 tcgttcactt cagaaatagg acacacggtt ccttttaccg gattttcaac aatgactgct
     5281 tcggctattg ccgtttgctc tgattcatca gaagtatcgt caaatccaat tagataagca
     5341 atgataaagg ttgcaacaaa ggcgattaca cacgtaataa ttgcgtgaac aatattcatt
     5401 ggattttctc caataaaggc tggtaaggct gctaagccag gggaaacaaa ggcatatctg
     5461 actaaaccgc tcaagccagc atacaaacca gcaattgcac caccaatcat ggaagcaact
     5521 aatggttttt tcagcttcaa ggttactcca tagagtgaag gctcagtaat tcccattaaa
     5581 gcagtgatcc cagtggacac agctagctgc ttcaactctt tgtttttagt tttcgcggct
     5641 acagcaaagg tagcacctga ttgagcaata ttagaagcca gcattcctgg cccattgatc
     5701 atttcagaac ccgtactgct caattgcata gtagcgattg gcgtcattgc ccaagctgta
     5761 cccgtaacca ccaaaaatgg ctgcaaacca cccatcaaaa atggaattaa ccaagctgct
     5821 ttgccgttaa tccattcagc ccccattgct ataagatcgt tcagcaacgt tccaaacggt
     5881 ccaatgacaa ccaacgctaa aggccccata attaaaaata ccagcatcgg cttagtaaag
     5941 aatttaataa acgaaggcga aattcgatcc gcaaagggct ctacatagga catgatccaa
     6001 acagtaaaga taatcggtaa aactgatccc gcataatctg cagctaatac cggaattcct
     6061 agaaaagaaa cggcatcgcc agtagaaaaa agctgtgaaa tatttggatg aagcaaaatg
     6121 ccagcaacag taatcgccaa aattgggttg acatcaaact ttatggctgc cccataggct
     6181 aatagtactg gcataaaata aaaggctgca tcagcaatag tattcaaaat gatgtaagtt
     6241 tgactactgt ctgatagtac accagtcaaa gtcaagacgg ccaaaatggc ttttaccatc
     6301 ccggcacctg ttatagctgg aatcatcggt gtaaaggtgg tagaaatgac actaatcaat
     6361 cttgaaataa tactttcttg attgtctgaa gaggggccct ttttaattga agttcccact
     6421 tccttttgaa tttccttgaa ggctgtttgg acttcattgc caatcaccac ttggtattgt
     6481 ccaccgctat ccaccacact aattacacca agtgtttttt ctaaggcttt cgtatcggct
     6541 ttccttttat ctttgagttc aaaacgcagt ctggtggcac aatgggttaa gctgaccaca
     6601 ttttcttttc ccccaacctg ttgcaataca tttcttccta cttctctgta atccattatt
     6661 ctctctcctt aaagcagtaa aaaagaccaa gtcaaaccac gccccaatca acattgaagt
     6721 gttagtttgt cttggtcttg cctgcataat cagtaacatg ccaataacgt tattttgctg
     6781 taatccaata atagcacatt atgatagcgc aatcaacagg gaatttaatt ttttatcttt
     6841 tcgtttgacc gtatagggta atataacagt tagatcacat caactagggt atatctgcgt
     6901 atacttactg ctaaaataag tatatctaat attaactata ccagtaaaac cattcctaca
     6961 taaccagcgg gaaattccca aaatcgtgaa agcatcaaaa atgcatataa cggatcacac
     7021 aacttcaaca attctgctct tgggaaaaat tgatgaattt ggtgagtaaa ggatggaata
     7081 aacagcattc caacaatata ggcaacaata gtgatacttg tttttttggt aaaaatcagt
     7141 aagcaatagc caatttcaat cattccgaga tataaaggta tcgatagaat gatttgggct
     7201 agtattctta aaaattaact agcaaatgtt taaactgatt tatttctaat ctacatgtag
     7261 taagtatttc aattccacca attatcaata aaaataccaa acatatccat aataattttt
     7321 tctttttcat ttcttttata ttgttataac agtgaataac ataaaaattc ccgacaatca
     7381 ttgtaatatt tttttgtcac ctgtattagt atttaattta taggaaacta taaaaactcc
     7441 ctagtgaatt tacacctttg ggagttcctg tttggacggt atgcataata catttaaatc
     7501 aatttaacat taacacctca gcatatggta tctccacata tatacggtct ttctttggat
     7561 agtactcaat atttatgttg atagcacttc cattgtctaa tatagcttta gcccggtatg
     7621 tgttatctcc attttcatga gtaatcgtca catcatcaca tgatcgattt aagttgtact
     7681 gttcttgtaa aatctgtgtt accaatggtt ttgatgactt ctctaatcca gatgtacctg
     7741 tcatttttga aaaaataata cctataatag ccaagacaac aatacctata atgacaaact
     7801 tcttgttcat atttacttta ttcaactttt tatctcctat ctccaaaata atttgttctt
     7861 atccctctta tcagtacatt gacatcccta aaagtacacc aaagattgct ccagctaccc
     7921 ctgctatcat caaaatcgta cccattttct catctttttc tcgatataag tagcccataa
     7981 taacagcaac agcaccaaat acaacaggaa taaatgctaa cgatactacc atagaaatcc
     8041 aaccaataat caaatacagc ttccctgtct cttgtccatg tacgatttgt tccttcattt
     8101 cattaattgt ctgctcagta cttttcttta ctgtgtctgt tccggttgtc tcatctgttt
     8161 cttgtagtgc tccacaaaac tggcaatact tactttcatc tgttatttct ttgccacatt
     8221 ttctacaata catgtcttct cctcctaaaa tcattcaatt gcttattagt taactgttta
     8281 ctttattaaa tacatcagaa aacagctcat tcattggtac acttaatatc tcagaaagca
     8341 tcttcatttg atagggttta ggaatcgttc tccctgtttg ccacgaactt acacattgct
     8401 gtgtaacatt catttttttt gctaactccg tttgagaaag tcctttttct tttctgtatc
     8461 ggctgagatt acttactttt ttcatgattc tcccctcctg ttttattata tacaacaata
     8521 tgatgtattt caactcataa aatataaaaa acttcaaata ccattacaaa tacaacaact
     8581 tgttgtacta tactgagatg ggggtgtatt aatgaaacag aaacaatatg caaaggtcat
     8641 tagagaaaag cgaaaagctc tcgggtggac acaaaaagaa ttagctgaaa aaatcttctc
     8701 tacacaacag gcagtagcaa gatgggaaaa ttctgtaaca gagcctaacc tcgatagctt
     8761 aacagctctt tctagagccc taggaacccc tgtaagccat tttctagaca atgttgtcgt
     8821 agattatgag gaagagtttt tggctctcta tcgttctctt agcactgaag atgcagttcg
     8881 aacaattgac tatatgaagc tattaaaaag gcaagaaaat gaacgcaatc agctattgaa
     8941 agattgaaaa ataaataacc caccgaactt taacggtggg tgtattgaga tgaaataaga
     9001 ttaagagcta tggataacct gtctagttta atacaattgg gtcttttctc ttccttactc
     9061 tccgtccatc atctcttgtt acacttgtaa attgatacag aggaactatc ccagctcctg
     9121 ataatatcaa ttcttttatt gtttcatcta tccctgcccc tttcgctaaa tatattaaaa
     9181 aactactact tgctttcaca ttgtatagtt tcttaatatc cttagaagct cgttcaagag
     9241 tgaatccttg tttaacaaag tagcctctgt tttttattaa agcatttttc acgctgttat
     9301 ccccaacatt acgtaggcgt tcacaaacag tgttaataag ttctttctct gtcattagat
     9361 atgtcccttg ccacgataag ccctgtaaaa cttcatgtgc ttcatctaaa atcaattgtt
     9421 tttccttgct gagttctgat cgggaatttt gaggaaatac tcttttagcc tctttttcgt
     9481 taaaacatat cttaacccat tcataagaaa aatattgata tgtaaaatga attttttcca
     9541 gaagtggaag gatatcttca ttcaattttt gaatcacaga agttgcatta aaaacgtcta
     9601 atcgatagac attgcttctc ttatgattaa agtttcctaa cgctttctct tcctttaacc
     9661 tatttaggta ttcgagcatc ttcaaaggaa catcttcatc atctaatttt tttatcaatc
     9721 ctataaaagt cataagtgat agtaacttat tacatttatc tatgtcataa gttttaccat
     9781 agtaagaact cattttttta gctaattgtt cccccgacaa aatacaagtc acactttcct
     9841 tactatcatc aaaaaaatta acatcagaaa gcaagacatc atagacacgt cgcatttcct
     9901 ctagataatt ccttattgtc ctgaaaagtt tgtctttcat tgattcaaga gtgtcaaaaa
     9961 gtacttttga ttcctctttc aattttcgaa aatgtgtagg atacaagtct aggttctcta
    10021 atagccaatt aaatgtttct acccgactgt ctttctttaa aattagcatc acaactccaa
    10081 taaaattttc actgatatca caattactgg aatgacaata aaaaatggaa tgcccagttt
    10141 tactggtaaa aatactagca gagggattat gatcgttgtg aaacagacat cttaagtttt
    10201 tacttgactt aagtaattta ttcatatcaa tggttaacaa tctttcataa atctcattaa
    10261 aagacatgtc ggaggaatca aacaggcagt ctttaaacca ctgtttcatt tcttctttgt
    10321 cgtcattttt tatcaactta acaaaatcgg tttcattgac tagttcatac tctctccctg
    10381 aatcactttg acaattttta gaacatgtca tggtaaaatc aatcggaagc tcagtaatat
    10441 ctaacatgtt gtcttcatta attattatca ctgcatctga cttatatcct ccaaaaaaca
    10501 ttctattcga agcatttaca ttgtcatcac acccggggat gttctttttc aagtattcgt
    10561 aaacagcaac cattttacca tagtcagtaa ttgcttcttt taatagaaag acaatcttgt
    10621 atttttctag attttcagag taccgaatac ttttttgtat cgcaaacgca gaattatgaa
    10681 ctaaattgat agataaaacc ttttctatgc ttaagtatct actatcatct ttactcaaat
    10741 ctactacctt ccccttctca tctttaacag tgttatcgac atccagaata attactctgg
    10801 tttccacaaa actctccttg tcattgcctt tattctttgt cttgcttgga ataaatgatt
    10861 gtgatgaatt tagtatctct acaaactcat ctcttgtaac ttctctatag ttatctaaac
    10921 acctatctct tacatcagtt ttttcttttt ttgtaactgg cttctctaat aatttttctt
    10981 gatctacata aattggaata ttaatcattg atgtttccat ttgtgtttcc tccttataaa
    11041 attctgttaa agactaaaac atacagataa taaaaacaaa ctctttatta ctatatgttt
    11101 tagtcttatt tgtttactca acctttatac cgttccatga aactatcaat atcagatttt
    11161 ttaaatctaa tgaccgtatc aatttttata acaggtaacc cgtattgaga aacatatttt
    11221 ctaatcgttc ctactgatac acctagatat tcacttgcct gctgatagtc catatactct
    11281 agagtgactc cagaaataat gtcttctagc ataggcttta gcactgtaat tatctctttc
    11341 acttgttgtt caacttgtat agaaacaatg ctatcaatga tttgtagaga catatttccc
    11401 cctcctttcg tgattgcttg aacaaatttt ctaagaatta tcttcgtatt ttttgctcaa
    11461 aataaaagta ttttgttatt aaccgattaa tcaaacaaaa aatgattacc agttcatcga
    11521 catacacata atacctcgat aaaaaagggg tgtcaacagc tttgtaaaat ttatttacaa
    11581 cacccctttt ttgaggtata ctaattttag gaggtgtggt caatatgata aaacgtccac
    11641 caataaatta tttagaacga aaaaagatac tcggaacaaa aataaaagct ataagaaaga
    11701 gtaaaaaatt aacacaacca gcatttggtc ttatgattaa caacggtcaa ttaattgaca
    11761 aaaaaactat ctacgaatgg gagaagggaa cctatttacc aataccagag cgattatccc
    11821 gaattgctga tttgggaaat atgagtatag aggagttagt ttgtggcaat gtagaagaat
    11881 atattttggg aattattctt tatagagata gcattgtact agatggtata acatttccag
    11941 ataaaaacct gttccagcat cttcgtcaac agttcccacc agtacactct aatttagata
    12001 catggctgga tagatattcc aaattagaac cagagatgca agaatttatt gctaacaaga
    12061 cttgtaataa agttaaaaat gaaaaaatta gtcttttcaa tatcttaaaa atagaagaat
    12121 tattcatcaa tgctattgta gaagaatttg acaataacat tcttttcctc actagcagta
    12181 tagaagaact actagaaaga atggtagatg aatggctacc tattcaattg aaagacatgt
    12241 cttatcccga agaagctgtg agagaaatta ctgataatat aaacaagcta gaacaaacga
    12301 tttcatcaat tgggaaaaaa tacactaaga aaaaaatgaa gggaggtgat accatctagt
    12361 ggtaagtact ccttgtaaac taagaggaga tgatttaatg gcaaaaatat caaagtacaa
    12421 aaagaaaaac ggtcaagtgg cgtggatgta taaaggacac attgccaccg atccacgaac
    12481 tggtgaaaaa ataaatacta cgagaagagg ttttagtagc aaacaagaag ctcaaaaaga
    12541 ttatgacgag tacagacatc aaatattata cggtgttaaa aagaaagcac ctgatatgac
    12601 atttgaagac ctgtataatg aatggataac tcatcagcgt accagtgtta aagcttcaac
    12661 aatcgcaatc tcagtaagat acgcaaataa tcaaatactt ccagcatttg gtaagcttaa
    12721 actatcgaat atctctgtcc catattgtca aaaagtggta gatgaatggc acagtaaata
    12781 tgaaagttac gattatatga gaaaacagac tgcacaaatt ttacgctacg gggttgcaat
    12841 gcaatatatt gacaataatc ctatggaaaa aacacttctt ccacgaaaaa aagagtatga
    12901 gaaaaatcgt aaattttact caaaagagga gcttaataat ttactggatg cttttaaaga
    12961 ttttggtaac atgaaacaat acgccttctt cagattattg gcatacacag gtatgagaaa
    13021 gagtgaagta ctcgctttac agtggaaaga catagacacg tttaataaag aactacatgt
    13081 caataaaact cttgctgtag atgaatttgg taaagttata attcaatctc cgaaaacaag
    13141 agcttctaga cgagttatct ctttagatac tgagactctt tctatcctca acaactggaa
    13201 attacagcaa aaagaagagt atttaaaact tggatacaat actagctcta aagagcaaca
    13261 tgtttttaca acagttaaaa acactctata cattccaaac actgttaatg attggttaag
    13321 atatattttg aaaaaatata acttgcctcg tattacacct catgggttta gacatacaca
    13381 tgctagttta ttattagaag caggagaatc tgtcaaggta gttcaacaac gattagggca
    13441 tgaaaatagt aaagtgacat tagacatcta tgcacatatt acaaataatg cacctaagaa
    13501 aacaggtcaa gactttgctg atatgatggc tcatcagtaa aagagctggt caaaatctgg
    13561 tcatttctag tatacaggca aaacaaaaaa gctagaaccc ttatatatca agggttctag
    13621 ctcctataac aggtttaccc aacactgcct tccatctcat aactgatcaa tcgattgagt
    13681 tcgactgcat attccattgg aagttctttg gtgaatggtt caacaaagcc catgacgatc
    13741 atttcagttg cttcagattc ggtcaggcct cggctcatca gatagtagag ttgttcttct
    13801 gagatttttg agactttggc ttcgtgttct aaagagactt ggctgttgtg gatctcattg
    13861 aatgggatcg tatcggattt cgaccattcg tccatgatga tcgtgtcaca ttcgatatga
    13921 gagatcgaac ccgcactgtt tttggcaaag gttacttgtc ctcgatagtt tacttctccg
    13981 ccgtcttttg aaattgattt agagacgata gagctagatg tatttggtgc attgtggatc
    14041 atttttgcac ccgtatcttg gatttggttt gctcctgcaa aggcgatgga gagcatggtg
    14101 cctcgtgcac cttttccatc taagtaaaca cttgggtatt tcatcgttgt ttttgcacct
    14161 aggtttccgt cgatccattc aacggttgcg ccttcgtatg cttttgctcg tttggttact
    14221 aagttgtaca cattgtctga ccagttttgg atcgttgtgt aacggcaata cgcatctttt
    14281 cttgtaaaga tttctacgat ggcggcatgc aggctgttac ttgaataagt aggtgccgta
    14341 caaccttcga catagtggat gcttgctcct tcgtcaacaa tgatcagcgt acgttcgaat
    14401 tgccccgtgt tttccgcatt gatccggaag tacgtttgta atggaacatc gactttgacg
    14461 gcttttggca cgtaaataaa ggtcccacct gaccatacgg ctgaattcag cgctgctaat
    14521 ttgttgtcgg ttggcggtac aagttttgaa aagtactctt tgaataattc agggtattct
    14581 ttcaaagcag agtcagtgtc agtaaagacg attcctaact tttcgaattc atctttcata
    14641 ttatggtaca cgacttctga ttcatattgt gcggaagcac cggctagata agctcgttca
    14701 gcttctggta taccaatctt ttcaaatgta tctttgattt tttctggtac gtcttcccag
    14761 tcacgagcgg gtttgtcact tggtttttgg taatatttga tggcatcgaa atcgatgtct
    14821 gaaaggtcgg ggccccatgt ttgcattggc attttgttaa atgtttctag agatttcaga
    14881 cggaattcaa gcatccattc tggttcgcct ttgattcttg acatctcacg gatcacttct
    14941 tctgtcaagc cttctcctgt actgaaaacc ggttcgacat catcgtgaaa cccgaattta
    15001 tattcttcta attctggtac gcccatgttc tcactcctct tccttgtggt aatgatggaa
    15061 agctgcggta ccatcgtttt ccattgcttt tcctagtgct ttccaggcaa gtgtcgcaca
    15121 tttgatccga gcgggaaatt tagctacacc accaagcata gcagcatcgc ctaattggtc
    15181 gggatctttg acttcttttc cttggacaag ctgtagaaag tctacagtta aatgttctgc
    15241 ttcttctatt gattttccga tgacggcgtc tgtcatcatc gaggcacttg ccgtactgat
    15301 ggagcagcca ctgccgtcaa aggcgatatt tttgatgatt ccgtctttta catcgacttg
    15361 caattcgatc acatctccac atgtggggtt gttcaattcg atcttgcttg tcgagtcagt
    15421 caagcttcca cgatgatgcg gatgggaaga atgatccaag atcacttgac ggtataaatt
    15481 gtctaattta gatagtccca tgttggaaaa actcctttgt tgccatgatt gcttcaacta
    15541 aacggtcggc gtcttgcgtt gtgttgtaga aataaaagct cgcgcgagca gtcgctgata
    15601 cttcaaggta cttcaatagc ggttgcgcac aatgatgacc tgctctgacg gctacccctt
    15661 ccatgtctaa agctgtcgcg acatcatggg gatgtaaccc atccaaattg aatgcaacga
    15721 ctcctgtccg aaaattcggg tcttgtggac cataaacagt caatccatca attgcttgta
    15781 attttggcaa cacataagcg gctagttctt cttcatatcg atggatattg tccataccga
    15841 gtttttgcaa ataatcgatt gcatggccta atgcgatagc gccggcgata tttggtgtcc
    15901 ctgcttcgaa tttccaaggt agttctttcc atgtactgtc gtataattcg acaaaatcga
    15961 tcatttcgcc accaaattca acaggttcca tttcttctaa ccattttcgt ttaccatata
    16021 aaacaccaat acctgttggt ccgcacattt tgtgtccgct gaaagcaaag aaatcacagt
    16081 ctagtgcttg gacatcaatg gccatgtgcg gggcagattg agcaccatct actacgatga
    16141 ccccgccgtt ttcatggaca agttcagcta attcttgaac aggattgaag acacctaaga
    16201 cattggatac atgagcaatg gagacgattt tggttttgtc cgtaatcttt ttcgctgcat
    16261 cctccatatc caggaagcct tcttctgtta gttcgatata tttcaagaca gcacctgttc
    16321 gttttgccaa ctgctgccac gggataacat tagaatgatg ttccatgtaa gagagcacta
    16381 tctcgtctcc tgctttgata tagcggtcgc cgaaggttct ggctacccaa ttcaagcttg
    16441 tcgtcgtacc acgagtaaac agcacttctg ctgtttcttt tgcatggatg aacgcccgta
    16501 ctttttctct tgcttgttca taatcatggg tcgcgcgttc tgctaacgta tggacaccgc
    16561 gatgaacatt ggcgttatcg tgttcgtaat agtacgctaa tgcctggagt acttgttttg
    16621 gcttttgggt cgtcgctgca ttatccagat aaactaaagg ttcatcattg acgtcttgaa
    16681 ataagatagg aaaatcattg cgtagctttt tagcatcgat catccattca acttcccttc
    16741 gattacttcg actaattctt tccgaacttg ttctacaggg attgctgtta agacagaacc
    16801 gaggaatcct cgaatcacta agcgctctgc ttcttcttta tgcaatcctc tactcatcag
    16861 atagtacatt tcttctggat caactcgtcc tacactggca gcatgtccag cagttacttc
    16921 gttttcatcg atcaaaagaa taggattcgc atcaccacgt gctttatcag aaagcatcaa
    16981 gacacgactt tcttgttggg catctgcacc ttttgcccct ttcaagatgt ggccgatacc
    17041 attaaaggtc aatgttcctt tttctcggat cactccgtgt tgcaaaatat gcccgatcgt
    17101 atgaggggct ttattggtta ctcgtgtatc gattccttgg atttgtctgc ccgagctgat
    17161 tgccacgatt ttgacttctg cgtgtgcacc ttctcctgct aaatctgagt caaagtcagc
    17221 gatcacatga ccgtcattca tgacaccaat tgcccagtcg acagaagcat ctcgtaaaat
    17281 gtgcccacgg cgattcatat aagaagtaat attttctcct aattgatcta cggctgaata
    17341 tttgattttt gaaccagctt tagcgatgac ttccacgatg atattacctg atgattttgc
    17401 tacttgttct ttggttgtct ggaaacgttc cagataagaa aattcactat gttcatctgc
    17461 cacgatcaac acatgcttga agaagtgctc atcggatgcg ccatcttgaa tgaataagga
    17521 ctcgagtggt tcatcgatca cgacattttt aggcacatac aggaaaatac cgctgttcat
    17581 aaaagctaca tggagtgcag tcaattggtc ttcgttcatg tttactgctt ttttcatata
    17641 atattcttgg actagttctg gatattcgat cattgcagtg aacaagtcgg taaaaatgac
    17701 tcctttttct gctaattcca caggcagttg ttcaaaaatc gtccatgaac cttgttggac
    17761 aagcacagga ttgtctttca ttgcatcaaa tgccggaatc gtgcctgttt cactgatggt
    17821 ttgtgtttct ttcacatcaa ataacgacca gcgatggaat ttcacccgat cgatatgcgg
    17881 caatggcagt tcatctgctt tcgctagtgc gtcttgacgg aaggttctca tccaatcagg
    17941 ttcgccttta cttaaagaaa aatcatttac cgcgtcaaga taatcgagcc agtttttttc
    18001 tttcattctt gctccccctt acgcttcttc ttctttatag tcgattccta attctttact
    18061 gataccagcg tagccttctg cttccagacg tttagctagg tcagcattcc ctgtcatgac
    18121 tactcggcct tccatcatga tgtgaacgac atcaggtgtg atatagttca ataatcgttg
    18181 atagtgggtg atgatcaatg caccgaaatt gtctccgcgc atttcattta ctcctttagc
    18241 aacgactttc aaagcatcga tatccagacc tgaatcgatt tcgtctaaaa ttgcaaatgt
    18301 aggttctaac atcaacaatt gcaggatctc attccgtttc ttttctccgc cagaaaaacc
    18361 ttcgttcaga taacgttcag ccatttcttc tggcatattt agtaaagcca ttttttcatc
    18421 taactttttc aaaaatgcca taacagaaat tttgtcgttt tcatcacgtt tcgcattgat
    18481 cgctgcacgc atgaattccg cattcgtgat acctggtatc tcacttggat attgcatagc
    18541 taaaaataag ccaagtcgcg cacgttcatc tacttccaag tctaaaacat ttttcccgtc
    18601 aaacaaaatt tcgccttctg ttacttcata gtttggattg cccatgatag cggcagataa
    18661 ggtagatttt cctgtgccgt taggtcccat gatggcatgg atttctccgg ttttcattgt
    18721 taggtttacg cctttaagga tttctttttc ctcgatcgac acatgtagat tttttatttc
    18781 taagacagac atgcattata cccccaaatt ctctttcgtt ctcactcatt ttagactaat
    18841 tgagaatgaa gcgcaatgac cttccttact aaaatcaaat atcttttatt tattattaaa
    18901 caattttgat ttctaaatta gaataggtat aaaaaagatg agaaataagc cattacagac
    18961 ttatttctca tcttttttgt tccatacctt attttgcatc agcctttact ggtttaacag
    19021 agccgcccca tttgtctgtg atccattctt ggacttcttt gcggtgaagc acttcaatca
    19081 gttttttgat tttgtcactg tcttcatcac ctttacgagt cgcaataata ttcacgtaag
    19141 gagagttgtc cttttcaagc aatacggcat cttctaatgg attcaaccct gcgccgtaag
    19201 cgaagtttgc gttgattgca actaggtcac cttcatcatt ttggtacgta gaagctaaaa
    19261 gtgatgggtc gatcgtatgg ttgaacttca agttcttagg gttttcttca atatcatcga
    19321 atgttgctgt ttccagatct actccatctt tgactttgac taagccagca tcttgtagaa
    19381 ttgtgatgat gcgtccccag tctgattcag aatttgaagt aatgattgtt gcaccatctt
    19441 ttaattcgga aacatctttg acttttttag agtacaagcc cattggttcg atgtggacag
    19501 caccggcatt gacaaagtca tatccttttt ctttgatttc tttgttcaaa taaggaatat
    19561 gctggaagta attggcatca atgtcgcctt cttccaaaga ttggttcggt aatacatagt
    19621 catcgaattt gacgatttca agatcgattc cttcatcttt caataatggt ttcacatgtt
    19681 ccaaaatctc tgcgtgcggt gtcggtgaag cgccgacttt caatgtcgtg tcgtctgctt
    19741 tatttgctga atctttgctg tcgctgttgt taccgcatgc tgctaatcct acagtcagca
    19801 aaagcgctgc tgcaaatcca aaaattttct ttttcatatc ctacatcccc ctatatccta
    19861 ttatcttttg tctgttttct tagttaagaa atcaccgatt ccttgaacga taaatacgat
    19921 tagtaaaata attaaagtcg caacgaatgt cactgttaga ttaccacgct ggtagccttc
    19981 ctgccaagca agacctccaa gtccacctgc gccgatcgcg ccggccatcg cagtaaatcc
    20041 gatcatcgag atcgttgtaa ctgttaatcc agaaatcaat gcgggaatac tttcaggtat
    20101 caagaccttc cagatgattt cccagtaact tgctcccatt gcatctgctg cttccagtac
    20161 accagggctg acttcacgaa aagcaatctc tactagacgg gcataaaatg gtgctgcgga
    20221 taaaacaaga gctggaatgg ctgcttgcgc acctagcata gtgcctacga ttgctttagt
    20281 aaatggaatg atcaaaacca tcaggatcat aaatggcgtt gaccggaaaa tattactgat
    20341 gatcgatacg attccataaa tgattcgagc atacggtttg tttttgcgtc caatagagta
    20401 aaggatcaat cccaaaacta atccgatgat cgtcactaag accattgaga ttaatgtcat
    20461 atatagtgta tcaaggatag cctgattcat cgtttccggg ttgatttgtt taaaatctag
    20521 atatgtttct gaaaagcttt tatccacgat ggatcacctc aattcctact ccttgttctt
    20581 tgatgaacac ttctgcttga tctaattctt tggtttcacc agtcatcaag acagtcaatg
    20641 aaccaaagga accttctttc acttgtttga tctttccata cacaacgttg acatcgatgt
    20701 tatagcgtcg aatcgcttgg gaaatgactg gctcgttggc atttctttcg ctaaaggtca
    20761 aagttgccaa acggccgctt ggattttctt tgatgagttc agctaatagt tctgtcgaat
    20821 cagcttctgg ttcgatgtct tgttgtacaa agcgtttggt tacttctttt ttcggttgtc
    20881 tgaagacagt caatacgtct ccttcttcta cgactttccc caactccatc acagcgactt
    20941 tgttgcagat tttgcggatc acattcattt catgtgtgat caagacgatg gtcaaattca
    21001 atttcttatt gatcgctaac aataagtcta atacttcttc tgttgtttgc gggtccaatg
    21061 cgctagtagc ctcatcacaa agcaggatct caggatcatt tgccaatgct cgtgcgatac
    21121 cgacgcgttg tttctgtccg ccggaaagtt gtgaaggata tgcttgtccg cgtccttcta
    21181 acccgactaa acgcaacagt tcttctgccc gttctttacg tttgctcttt ggtacgccag
    21241 ctaattccag cggcagctgg atattttcaa gtacagttct tgaccataac aggttgaaat
    21301 gctggaagat cataccgatt ttctttcgga atgtacgtaa ttctttgttt tttaattttg
    21361 taatatcttg atcgtttacg atcacttcgc cgctcgtggg aagctcaagt ccattcagga
    21421 gacggactaa tgtacttttt cctgcgccag aatagccgac gattccataa atatcccctt
    21481 tttcaacgtt tagtgaaacg tcattgacgg cttggatgtt tccgtttttc ccattgaatg
    21541 tctttttgac attcgttagt tggattaatg acatgccatt cacatctttc tcttatgatt
    21601 tttcaaacaa aaaagccttc gccctcttgc cttaagcaaa aggacgaaag cttgaacttc
    21661 cgtgttacca ccttgattta ccgttacttc gcaataacag cctcttccag tacttggact
    21721 tagcttcttt ccgtatactg tcgcgctgta acgggcactc ccgtagtaag tttgcttacg
    21781 ctcgcttact ctgctctaag accatcttcc atctcatttt cgttacccat tttcagcaaa
    21841 cagggctctc ttcaaacgtc cgtgacatgt actcttcttt tcaaagcttt ctcgtttcat
    21901 ataggattgc ttatgattat agcaactgga agaatcagtg tcaacttttt tcaagtgatt
    21961 ttctgatttt ataattcgtc aaattctttt ggatcaacat ctttgaaact taagatccac
    22021 gcattcattt catctgaatc attcaagata ttgatatctt caccgatttt atcattaaca
    22081 gaagaaactg tacccgttag cgggctggca aattcattga ccgctttttc cgcttcaact
    22141 tcgatcagag gttcatcttt ttttagcgat tgtcctgctt ttggcagatt ggcaaacgtg
    22201 atgtctccaa gttcttcttg cgcttcattt gtcaaaccaa cacagtattc ttttccatta
    22261 aataagatcc aaaggttgtc ctttttcttc aaatgcttgc ctgccatttt atatcagttc
    22321 tccttcataa attttttcat tgaatccaat tgcaacaagc tggtcctctc gtaagatcag
    22381 tggtcgcttg atcagcatac catctgtcga taagacttgg ctcgcttttg aagccgtaaa
    22441 ctcgcctact tgatctttca gtccaagttc ccgatatttc atcccgcttg tgttgaacag
    22501 gcgacggatt ggtacttgac tcgtttctag ccaagtctca atcgttgagg ccttcggtac
    22561 ttctttgatc atatcaacgg tttgataact aacgccatgc tggtctaacc acgctttggc
    22621 ttttttgcat gttgaacatt tcgggtacca gtaaaaggtg tacaattttc ttacctcctt
    22681 gaagatcttg caggtgcttc attttggtat ctcatcgata atatcaaccc gataccgatc
    22741 atgttcccta aaatcgagga tcctccctga ctaatgaaag gcaatgggat ccctgttaaa
    22801 ggaagtaatc cgatatttgc tccaatattc tcaaagacat ggaacagcag catcatgatc
    22861 aaaccgcttg cgatataggc gtaaaattca ttattcgtat cgaaacacac ccgaatcata
    22921 cgatagatta aaataaaata caagaaaatg acgaatgcac caccgataaa gccgaagttt
    22981 tcaccgataa cggtaaaaat catatcggac tcacgaacag gcacatagat attgcttacg
    23041 ttgaatcctt taccaaacaa gccaccggta ccaattgcca aaagtccttg ggccggctga
    23101 taactcattc ctgacgtatc atggaaaggg tccagccatg aatcaatacg tgcaaactga
    23161 taagatttga atccaacttt ataaagcaag tctcttccgc cttcagtcgt aaccaagaag
    23221 atcgttcctg ctccaacgac tactgccaag attactacag gtgcgatgat gcgccaagag
    23281 attccagaca tcaggaaaat cccgccaaag atcgcaagaa agacaagcat cgttccaaag
    23341 tcatcttggg ccatgatcaa tgctaaaacc ggtattgtca cgatcaagag cttcccaatc
    23401 agccaaaagt ccgatttcat cgtccgttct tttacttgcg tattatgttt agttacgatc
    23461 agtgccatca tcaagatgta cgcaatcttc atcaactctg ctggctggaa agtaaacgca
    23521 ccgaatcgaa accagttttt agaccctgtt gaagcagcta aattctgatc atagaatttg
    23581 agcaagagtc ccattaccac taaccccaaa ccataaaggt aaggagtcag tttccagatc
    23641 catttggaat tcaaatgcat gatgacaacg attgctgccg ctccaactaa ataccagaca
    23701 acttgcatcc ctacgccttt caagactgct ggattgttcg gatcatggct aagtgctaca
    23761 tataatgaga gcagaccaat caaacataac agaaagactg gtaaaatcac gccataatca
    23821 atacgattat cgttcgaagc ttttaatttt ttttccatgt ttcatccctt ttcttaaaaa
    23881 ccatactgct gattattata gcggtccagt tagataatgg aaagtctttc catagacatt
    23941 tgggaaaaat taactttccg tttgtgcagt tgcttgagca aatgcagtac aaaagcctac
    24001 tgcttatctt tagtgtatca tagaactttt tttgatacat gcaattatct accagctgag
    24061 attctcctcg ctgtgagaag tttctgtgct ttcattgtat cgaaggatct cttcggcaat
    24121 ctctagcagc tgatcagaaa ccagattggt tcgttggatg aaataaaaat atcgatggaa
    24181 attcaacgga gtcattggca cattttctgg aacagagcgc ttagacaaaa tcgagcgacc
    24241 gattccttcc ttcagcaacg caacaattac atcgttactt ttcacgatca tcttcttatc
    24301 ttggatattc atctcttcaa aataacgctg cgtgtaatga aaaacaccag agtttgcttc
    24361 tcttacaagc cacaatttgc tttttgggtc cccagcgatg actagctggt cttccatcaa
    24421 agagtatctt tgtacgccgg aagtctctaa tggcttttcg ataaaaccaa aatcggattc
    24481 atgtttcgct acttgttcta atgattcatg agaattcgtg atttcgatct caaaatgcac
    24541 tttaggaaac cttttgatca gatgtttcat caactgaggc aaaagataga cagcaaaggt
    24601 gtgggaagca acgatccgac atacttcttc ttcattttgt tcattgagca atttttgctg
    24661 agtttcttcc cattcttcta acaaaaccag tgtccgttca tataataagt cggcttgctt
    24721 agttggaagc atctcttttc ttccgttacg ttgaaaaaga tcgatattca attcccgctc
    24781 caactgtttg atttgattcg agactgctgg ttgagataaa aacagtactt ctgctgctct
    24841 ggaaaaattt cttgtttcat atactgctcg aaaagtcgtt aataacttaa acatggcacc
    24901 acttccataa ctttttatga taattactat aaaaactatt tgatttaaat atacaaaaaa
    24961 ccatctataa tagaaatgta caacaaaaca gttgctgatc caactagtaa cttagatgaa
    25021 gacaatcagc tgtctgcttg ctcgtaatca tacattccta ttcattttac aatgtattcg
    25081 ttcatcttgg tactaaaatc tattaaataa aaaggagaat attatatatg aagaactttt
    25141 tgaaaattat tccgatcccg atctgcgggt tgatccttgg gttagcttcc cttggaaatc
    25201 tgtttaaaga ctatcaccat gttgctttag gcaatctaac tggcggtatc tctatgattc
    25261 ttatgatatt catttttgtt aaattgttta tcttattcga acacacgaaa caaacgttgc
    25321 atgatccaat catcgcctcc gtttcaccaa cttttacgat gtcactcatg gttatttgta
    25381 cttattttgt ttcatatgaa agtattgcac caattgtcaa attcatttgg ctgtgcgctg
    25441 ttatcttcca agtgatcctt gtcttttatt tcaattatca ccatgttatc aaagctgatc
    25501 tttcaattga agcaatttat ccaagctggt ttatcattta cgttggattt ggggtcatca
    25561 ctgtaactgc cgggaacttc ttcccactta ttggaaaaat tttcttttgg atttcattga
    25621 tctgctatgc gttcttattg ccgatcatca tcaaacgtgt ctttttcgta agaaatatgg
    25681 ctcatccgac acgtccgctg attacgatcg ttgcagcacc tggttctctt tgcctaacag
    25741 gttatcttaa aaactttgct gaacctaatt tttatcttgt cgtagcattg ttcgctttat
    25801 ctcaagtatt gtatttcgtt gtgctgtcta tgcttccacg tttgttcaag cttcgttttt
    25861 atccaagcta tgcagccttt acattcccgc ttgttatctc tgcaacagct atatttacta
    25921 ctgttcatta cttcaacagt ttaggtatct atagtagcgt attggatttc ttaaaagttg
    25981 cggaatgtct gtttgcaagt gtagttgtct tctacgttct tgctcattac gtacgtttct
    26041 taattaaaga acatagcaaa catggaaaat ttgtttctaa aaaaatcgct taataatcga
    26101 ttcaataggt tgtgaaaaag ctgttctgca tcaagtaata agaacggcca aacaaaaaca
    26161 gctgttcatg tttttgtact ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    26221 tttaccaaga ggtcgtaa
//
LOCUS       DACEDO010000041        25869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEDO010000041 DACEDO010000000
VERSION     DACEDO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.041"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..691)
                     /locus_tag="IWO52_001711"
     CDS             complement(<1..691)
                     /locus_tag="IWO52_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0355980.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEE"
     gene            839..2392
                     /locus_tag="IWO52_001712"
     CDS             839..2392
                     /locus_tag="IWO52_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0355981.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            complement(2500..3582)
                     /gene="uxuA"
                     /locus_tag="IWO52_001713"
     CDS             complement(2500..3582)
                     /gene="uxuA"
                     /locus_tag="IWO52_001713"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0355982.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3573..5219)
                     /locus_tag="IWO52_001714"
     CDS             complement(3573..5219)
                     /locus_tag="IWO52_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0355983.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5403..6065
                     /locus_tag="IWO52_001715"
     CDS             5403..6065
                     /locus_tag="IWO52_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0355984.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            6209..6772
                     /gene="ahpC"
                     /locus_tag="IWO52_001716"
     CDS             6209..6772
                     /gene="ahpC"
                     /locus_tag="IWO52_001716"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0355985.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            6782..8311
                     /gene="ahpF"
                     /locus_tag="IWO52_001717"
     CDS             6782..8311
                     /gene="ahpF"
                     /locus_tag="IWO52_001717"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0355986.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            8468..8818
                     /locus_tag="IWO52_001718"
     CDS             8468..8818
                     /locus_tag="IWO52_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0355987.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            8917..9447
                     /locus_tag="IWO52_001719"
     CDS             8917..9447
                     /locus_tag="IWO52_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355988.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            9601..10752
                     /locus_tag="IWO52_001720"
     CDS             9601..10752
                     /locus_tag="IWO52_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0355989.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(10861..12900)
                     /locus_tag="IWO52_001721"
     CDS             complement(10861..12900)
                     /locus_tag="IWO52_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0355990.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(12958..13515)
                     /locus_tag="IWO52_001722"
                     /pseudo
     CDS             complement(12958..13515)
                     /locus_tag="IWO52_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(14054..14260)
                     /locus_tag="IWO52_001723"
     CDS             complement(14054..14260)
                     /locus_tag="IWO52_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355991.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(14276..14890)
                     /locus_tag="IWO52_001724"
     CDS             complement(14276..14890)
                     /locus_tag="IWO52_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0355992.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(15037..15720)
                     /locus_tag="IWO52_001725"
     CDS             complement(15037..15720)
                     /locus_tag="IWO52_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0355993.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(15829..16287)
                     /locus_tag="IWO52_001726"
     CDS             complement(15829..16287)
                     /locus_tag="IWO52_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0355994.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(16371..18134)
                     /locus_tag="IWO52_001727"
     CDS             complement(16371..18134)
                     /locus_tag="IWO52_001727"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0355995.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(18355..19815)
                     /locus_tag="IWO52_001728"
     CDS             complement(18355..19815)
                     /locus_tag="IWO52_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0355996.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(20023..20949)
                     /gene="glsA"
                     /locus_tag="IWO52_001729"
     CDS             complement(20023..20949)
                     /gene="glsA"
                     /locus_tag="IWO52_001729"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0355997.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(20972..22399)
                     /locus_tag="IWO52_001730"
     CDS             complement(20972..22399)
                     /locus_tag="IWO52_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0355998.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(22430..23644)
                     /locus_tag="IWO52_001731"
     CDS             complement(22430..23644)
                     /locus_tag="IWO52_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0355999.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(23985..24227)
                     /locus_tag="IWO52_001732"
     CDS             complement(23985..24227)
                     /locus_tag="IWO52_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0356000.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(24259..24816)
                     /locus_tag="IWO52_001733"
     CDS             complement(24259..24816)
                     /locus_tag="IWO52_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0356001.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(24829..25017)
                     /locus_tag="IWO52_001734"
     CDS             complement(24829..25017)
                     /locus_tag="IWO52_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0356002.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(25030..25596)
                     /gene="amaP"
                     /locus_tag="IWO52_001735"
     CDS             complement(25030..25596)
                     /gene="amaP"
                     /locus_tag="IWO52_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0356003.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         7583 a         5041 c         4516 g         8729 t
ORIGIN      
        1 tttcttctgg gtctacatct ggtttttctc caggggttat ttcaggtttt gattcattgt
       61 ctggctctgg ttgagtttct gtatcgggtt cttctccagg ttctatttcc ggtttctgtt
      121 ctccgtctgg attttctgta ggcggtactt ctggcttatt ttcattttct ggtttatctg
      181 ttggttcttc aacaagatct cctcctgagt cgatagctga atcttgctca attgtctgcc
      241 gctgactata cggcgttaaa tgatgatgat cgttcggtag tccatctgat accttgacag
      301 taaaaagcgc tggttttaac aagtcctgat ttaatgtaac ggtgaaggta aatatcgctt
      361 gcttgtgttg gatagtcaaa gtgttctcgt catagccaat cgacaaagcg ggatcaacat
      421 tctcaaatgt aacagctgat tgtagtatag gtgctaagtc tcctgaatag tcaatttttg
      481 ctttgatggt cgctgtttgt ccttttctta attttgcgac tcctgaattg aaggatgaaa
      541 attcatcctg aaaggtaatt cgattgacgt tggcccactt ggtttctgtc catttggctg
      601 tttctatctc ctctgtgtgg actgtctgat ttatagtaag cgataagctt aaggctgcaa
      661 atgttgatat taagatggtt gaatatctca taactctctc actccttgat ttttttgtat
      721 gcaagaaata agatagtaga gattctacct atatgtttac tgtttttttg tctaaattta
      781 tgaaaaaaac agtaatattt tcaagtttta acaatttaag taaaaaggag cctctttcat
      841 gcaaactcaa gaagcgatta aaacgtttct cttgaagaaa aaaagcaata caaaattaga
      901 tatttttctt ttcctatctg aacatcgttt ctttattact acacaatatt tagcagatca
      961 tttccatatg tctgaatcaa attttttact atacatcaaa gaattagaac aagactttga
     1021 gcgtctcaat ctaacagaac tgcatattga taagcaaaaa ccttttttaa agttgaattt
     1081 tgaaggaatt gatccggctt actgctatta tcgtctattt ggaagatact gcaacgaatc
     1141 agtcagttat cagatcttga cttctttgtt cagctgtcag acaaattcga ttatcagttt
     1201 aagtcagcaa acaaactata gtgcctctta tttgtatacg aaaatgaaaa agattaatgc
     1261 atttttagca ttgtacggtt tgtctatctc cttttctaat aatggcagaa agtctttttc
     1321 tggaaaagag gtacaaattc aatatgcagc attagatatt tattggaaca tcttttcaag
     1381 tactgctaca gaattttatg atgaagatcc gcaagtagtg gcttttatga tgaatacttt
     1441 ttttaaaaaa gaagtacttg accgactaaa tagcggtatg atagataaat tatattttct
     1501 attaaaaata tgtaaagaga attttcctgc aacttctgtg aaagacgtac aaaaagaatt
     1561 cacagagtat gaatatattg acttttttat tgatccctct gtagatattt tgcgacctaa
     1621 tctgccaatt tgtcgcgaac aacgcatctt agtgaatatt ttagctcgac tttctatcac
     1681 gaaaatcgaa tcggaagaaa caagcttgga gcaatacaaa cttttaaaaa aagcaaacgt
     1741 tcctcatgtt ctttattcaa gagaaatggt ggagtccttt tgtactgtat ttgatttgtt
     1801 tatacctgaa aaagaaaaga ttctttactc attggcattc gcacgaaata aactttataa
     1861 tggatttctt aacttcaatc aaccaaatac gccccttcct actttcatgc tttatcaaga
     1921 acatagcgtg ttgaaagaag tccaaaccgc cattgaacaa ttttatatgg atttcagaga
     1981 acaaaatcaa cacttgttac cagttatttt agaaaaggaa aatgtgcaat ggatggttga
     2041 agacctagta catttgtatg atcgctacaa aaaacagccg cgaattgtga ttggtgtgaa
     2101 ctatacgcgt gatttttata tcagtaaaga tctgatcgtt aagatagaac aaattttttc
     2161 aaatgaatcg atcaaaatac aaaatgattt tatggagaac tgcaacatcg ttatcagcga
     2221 ctgccctttg aaacaattgc cttctcatat caaaaaaatc tatattttaa aaggaatcat
     2281 cacaccagaa gactggaaaa aagtgattat gaggatctca gaatatatct ttgaattaaa
     2341 agaagaagca ctcatggatc aatcgaaaaa agaaaatcaa gttacttcct agaccaaata
     2401 agatacataa atccgaatgc ttattaacaa cctccggatt tatgtatcaa gaatttgaac
     2461 atccttctta tttacataat aaaattacag tttttttcac taattatctt tacttttgtt
     2521 tcctttttcg attgcttccc ataatccatt tagataaaca gcacctaatg ctcgatcata
     2581 taagccgtat cctggtcttc ctgtctcatt ccagatcatt cttccatgat caggacggat
     2641 gtattcgtta aatccgttgt catacattgt ttttagtatg cggtacatgt ctaatgaacc
     2701 gtattcggat ttatgcggtg cctcatagaa atctcgatta gctaaaaatt taatatttcg
     2761 aacatgtgca aatgggattc gatctctttg acaaaattct gctaaaatcc catatacatc
     2821 attttctgga tcttctgcaa tactccctgt acaaagagtg attccgttat aagggctgtc
     2881 tactatgcgg cacaaccaat ctaaatcttc tcgattcttt acgattcgag gtaatccaaa
     2941 aatcgagtat ggcggatcat ctggatgtac agccattttg acccctacct cttcacatgt
     3001 tgggatgatt gattggagaa aataggcaaa attttctcga agtttttctt cgtctactga
     3061 tgcataggct tcaagcaagg atttgatatg agctaagcgc tcaggttccc atcccggcaa
     3121 ttcaaattca ttgctggaat gttcgatgcg cgcgacgatt tctcttggat catctggaat
     3181 atcatcagat ataaaagcta acgtggatga gccgtcttct aagcggtagt caaggtcaga
     3241 tttcacccag tcaaatacag gcataaaatt atagcagatg acttctattc catattctgc
     3301 caagtttcgg attgtctgct tatagttttc gatatactgc tcacgggaag gtaatccaat
     3361 tttaatggca tcatgcacat tgacgctttc aattactttt agtgttaacc cacgagactc
     3421 aatttcttct tttagttctt tgatcctctc cctgggccat acctctccta ctggtatatc
     3481 catcaatgtg cctacaatcc ctttgaccgc aggaatctgt ctgatttcat ctaaggtaat
     3541 cttttcttcc tgtgatccgt accatctaaa agtcatttcc atgataaatt tcctccatgt
     3601 tcttgtaagt aatcctgtaa tgtttttcgt acggcattcg gtcctgctac catcttgatg
     3661 aacatttctt ctatttttct gccaagctct gcttggtaaa gatctacacc aaaaatttct
     3721 ttatttgaca aaataggagc cattttctca tgaatatgtg atgtatcttg cgtacccagt
     3781 tgaatatcgg aaagcttatg ctgaaagtct gtcaatagtg ggtctggact cgggatgaat
     3841 ggttttcctt gatcatcaat gccaataagg tatcgaagcc aagcagcgag agtcaatggt
     3901 atgaatacaa gtgtctgcgg gtctttaact tctatttcca tatatttttt aatcgtctca
     3961 ccataacgaa tcgccacctt ttgagaggta tccgtggcga ttcgctgagg catatcagga
     4021 atatttggat tagaaaagcg cacagtaatt acttcttcca gaaagatctt tggatcaatg
     4081 atacctggag actccacaac tgggaggcct tcattgtacc cgatttcaga aactagcagc
     4141 gataaatctg gatctttcat ctcctcagct atgctcctat accccagtaa acaacccata
     4201 atagctaaag acgtatgcaa aggattaagg caagtcgtca ccttcatcgt atctgcttta
     4261 tctaccgttg cacgatcagt caagaatacc ccagcttttt cgaatttcgg acgtccattg
     4321 ggaaaatgat cttccatgat taaataatgg atgtcctctg tattgacaaa tggagcaata
     4381 ttatttcctt ttgcggtttg gaaaatcgac atatttgaaa agccctcttt tgccaacatt
     4441 ttgctaactg acaaggaagg attcggggtg atacgatcaa tcatcgtcca tggaaacgca
     4501 acaatttctt cattcgataa gtattggata aatgtatttt gtacataacc ttccttttgc
     4561 cattcttttg cgatcgttag caaagaagta cgtaattgac taccgttttg tgaaaaatta
     4621 tctgtagaga caagtgcaat cggccaagct ccagataaaa aacgtttata aagtagcgca
     4681 gcaagaatac tcattgtatg tgatggcttc tttggaccat tttttatatc gactgcaatc
     4741 ggtcctatat atttattatt tgtatctttc aatcgataac ctttttcagt aatagcaaaa
     4801 gtaactaatt gtaaactagg attttcaaag tgtcgaatca tcttatcaaa gtgttcagaa
     4861 caatctggat gacaatacag actgtcagct gtcgcagcta atatacgttt gacgaactct
     4921 ccactgcttg gcatgaccac ttcaagaatc aaatcatcaa aatcttggta agccctttca
     4981 attatttctt catcgaatgt ttcacaaaca atgacaccag tggacaattc gcctagatcc
     5041 gctaaacgtt gagcgatttc tgcatgaaat cctcgataaa gatttccgcc accgaaatgg
     5101 atccagacag gatttttttg tccattttct cttaatgatg cacggtcaaa atgaggtatt
     5161 tctacttctt tttgagataa ttcagggata aagttcctcc agttgtttaa atcaatcatt
     5221 tttcccctcc taaactaaca tattagtttt tgtattcaag tgaattttat acaataaaaa
     5281 tcgctatgtc aaccaaaatt ttactattta tacaaattta ctagttaatg tgacatacca
     5341 gattgttttt cagacctctt ctgttatact acgttcatag aaatgatttt ggaggaataa
     5401 agatgaaaag cagcttgcaa tcacaagctt atcaagcgat tcgtaaacaa attatctatg
     5461 ctgacttaga acctggaagg aaaatatctg aaaaggcact ggaggaacat cttgctatcg
     5521 gccggactcc cattcgcgaa gcattgattc aactacgtca gcaagaactc gtgtatacga
     5581 tacctcaaag tggtacgtat gtttccaaaa ttaatcttgt ttctgcagaa aatgctcgct
     5641 ttgtccgaga acagttggaa gagcaaatca tgattgaatg ttgttccaaa ttggacacac
     5701 aaacacgaat agtgctagaa acgattttag aagaacaaga aaaagcagtc aaaataaatg
     5761 acgaacgcgc ttttttccgt gcagataatt tatttcatga agcttgtttc gaaattgcag
     5821 gaagaaaaga agtttggaac tggttagatg accataatac ccatttagaa cgtttccgct
     5881 ggttacgagt gaccattcag gggcttccat gggaaacgat catggaacaa cattatgatt
     5941 tactcaaggc tttagttgat aaaaaaccag aagaagctcg atttcaaacg ggattgcact
     6001 tacacatgat gctgaaagaa caggaaactg ttactcaacg ttatcccgag tatttccagc
     6061 agtagcattt tcttgcgacc tcttctattt ttataattaa ccttcgtttt tgagtaatct
     6121 ttattaaata acaaaatatc cattgacttt gcaactccaa ctaaattaca ataattataa
     6181 ataaataatt attattggag gttttactat gtcattgatt gggaaaaaaa tcgaagaatt
     6241 taatgcacat gcttatcatc aaggagaatt tttagaagtc agtgaaaaag atcttatggg
     6301 gaactggagt attctttgct tctatccagc tgattttact tttgtttgtc ctactgaatt
     6361 agaagactta caagaacaat acagtacatt gaaatcttta ggtgttgaag tcttttcttg
     6421 ctctaccgat acccacttta ctcacaaagc atggcatgat acatcagatg ctatcggtaa
     6481 aatcgaatac gtgatgatcg gtgatccgtc tcatcagatt tctcgtattt ttgatgtttt
     6541 ggatgaagag caaggattag ctcagcgagg aacatttatt attgatccag acggaatcgt
     6601 tcaagctatg gaaatcaatg cagatggaat cggtcgtgac gctagtgcac tgattgataa
     6661 gattcgcgct gctcaatatg tacgtaccca cccaggtgaa gtttgtccgg caaaatggaa
     6721 agaaagtggc gaaacattga aaccaagttt tgatcttgtt ggaaagattt aggtggtgga
     6781 tatgttagac aaagaaacta aacaaaatct cgaacaatat ttagcactga ttgaatctcc
     6841 tattgttttt tctgttagtt tggataatag tgaaaatagt caaaaactag ctgaatttac
     6901 gaaggaaata gcagaaatgt ctcctaaaat ctcttgggaa aaaagagatt cgatgcgcac
     6961 acctagtttt tctatcaatc cagttggcaa agaaagtgga atcgtttttg ctggtatccc
     7021 cctcggtcat gaattttcct cttttttact ggctatgtta caaattagtg gacgggcacc
     7081 gaaaatcagt gaatctttga gtcttaaaat caaaaaaata acacaaacat tgcattttga
     7141 cagttatgtt agcttgactt gtcataattg ccccgacgtt gtacaggcat tgaatatatt
     7201 agctgtatta aatccaaata ttactcatac gatgattgaa ggtgggatgt tccaaaaaga
     7261 aatcgatgat aaaaaaatca tggcagtgcc aactgtgttt ttaaatggag aagaatttag
     7321 tagcggacgc atgacgatcg aacaaattgt tgaaaaaatc actggccctt tgattgaaga
     7381 agagttgttt gaaaaagaac cttatgatgt attagtaatt ggcggtggac cagcagctag
     7441 tagtgcagct atctatgcag cacgtaaagg aatccgtaca ggacttgtcg ctgattcttt
     7501 cggcggacaa gtcgttgaaa cactagggat tgaaaatatg attggtacct cttacacaga
     7561 aggtccaaaa ttgatgcagc aagtagaaag tcatgtgcgt agttacccta tcgacattat
     7621 gatgaatcaa caggccgttt ccttaaataa agagcaacat tttattaata ttggcttagc
     7681 taacggtata tcacttaaag caaaaaccgc catcatcgcg acaggtgcgc actggcgttc
     7741 tatcaatgta ccaggcgaaa aagagtttaa aaataaagga attgcttatt gcccacactg
     7801 tgacggccct ttatttaaag gaaaagagat tgctgttatc ggtggtggta attctggtgt
     7861 tgaagcagca atcgatttgg ctgggttagc aaaacatgta tatgttctag aattcttacc
     7921 agaattaaaa gcagatcaaa tcttacagga taaactagtc tctcttgcga atgtcactgt
     7981 tatcaaacat gcagcgacga aagaaatcag cggtacgaat caggtagaat ctctctccta
     8041 tatcgatcgc cgtacgatgg agatgcatac actagctgtt tcaggagtat tcattctagt
     8101 cggcttactc cctaatacgg attggctaga tggaacgatc gaaatgaatc caagaaatga
     8161 aatagtgacc gagaaaaatg gcgcaacgaa tatccccggc gttttcgcag ctggtgattg
     8221 tacagatagt ccatacaaac aaatcattgt ttcaatgggt tcaggagcta ctgcagcttt
     8281 aggagcattc gattatctga tgcgcacttg agctatgaag attgtaaaaa gtcagagagc
     8341 ggaaaaaagg atggtgacaa gaattttgtc acaatccttt ttgttttttt gaataatcgc
     8401 tttttcttct ctttttctta tatactataa gtagtaaata atttgaaaaa aagaaaggtg
     8461 actgattatg gcgcaaaatc gtatttttac gacaagtttt gcaagcgttt atcctatgta
     8521 tgtagcaaaa gcggagagaa aagatcgtat aaaagaggaa gtagatgaaa tcatttgttg
     8581 gctgacgggt tattcccaaa cagaactaga atatcaacta caaaaacaag tgactttcga
     8641 acaatttttt gcggaagccc ctgtccccaa tccttcacgt tccttgatta caggtgttgt
     8701 ttgtggtgta cgagtagaag acatcgagga taacttgatg aaagaaattc gttatttaga
     8761 taaaatgatc gatgagctag caaaaggcaa aaaaatggaa aagatcctca gaagttgatt
     8821 tttccagaga tattcttctc cttcgctcat tttgtaacat ttaatttggt aaaatgacta
     8881 gttcacgtta aaataagtca aaggaggttt tcttatatgt ttgagacgat taaacaaaat
     8941 attcaaaaat acgccttatt aagagctgct gtttatatta tttctgggat tgcgattgtg
     9001 ctaaaccctt ctgctgtttt tcattttatc ggttatctga tcaccgccta tcttgttttg
     9061 cttgggttgt tgaatctttt tgaggcttac cgttttaaaa ggaaaacagg ttcttggagt
     9121 atcggtctgt tcagtggtct ttttttcctg atcttagctc tgatcgtcta tgcttttgca
     9181 cctgcaatcg tttctatcct gccgattctt cttggattag ctgttatcat caacagcttg
     9241 ttccagttgt tcttcagcat gaatacaaaa gctaaaagtt ggagtgttta tagtagtatt
     9301 ctgttgttga ttggcggatt cgtcttattg ttcaatccat tcaaaagttt gatgcttcta
     9361 ttccaaatat tcggctttat tttgattttc atgggcatcc aggagattat caatttcatc
     9421 agaataagaa aaatgactat acgttaatca tttttagtta ttaaaataga ccgtataaaa
     9481 accaacaatc caaaaaaatc ttttggattg ttggtttttt tgtgagtata ttttcctatt
     9541 ttttcaataa tgtatgttat gctaagttcg ggctatattt tatacaaagg aatgatgaga
     9601 atggaattta ttggtatttt atgcctgata ttgatttcaa caacgattgg ctcacatatc
     9661 tcgcggagat taggtatccc agcagtgatt ggacaattac ttgttggtgt tttgctaggc
     9721 caagctggat tgaattgggt caaacctgat attctagtcc atgacttttc ggagattggt
     9781 gtgatcttat taatgttttt agctggtctg gaaagtgatt tatctctttt gaaaaaatat
     9841 ttccgacctg gtatgttcgt tgctttgctt gggatcatct tcccggttgt ttttggctgg
     9901 ttgacaggca aagcattcca agtttcggga aatgaagcga ttttctttgg gattattctt
     9961 gcggcaactt ctgtcagtat ttcagtagaa gtattaaaag aactaaacgt agtgaataca
    10021 aaagaaggat caacaatttt gggtgcttct gtggtagatg acattttagt cgttttagtt
    10081 ctaagtttca gcctatcttt tctaacgggg gaagcttcta gcaatgcttc tttaccgctt
    10141 gttttgattg aacagctaat ttattttgtt tttattttct tattagtcaa atggattgcg
    10201 cctttcctac tttcatttgc agaaaaagtg tacgcaaatt cttctattat catcgtatcg
    10261 ctagttattt gtttaggaat gtcttacgta gcggatcttg ttgggcttag ctctgtcatt
    10321 ggtgcttttt ttgctgggat agctgtcagt cagacaaagg ttaaagaaga agtataccac
    10381 aatgtagagg ctttaggata tgcagtattt ataccagtat tttttgtcag tgtaggatta
    10441 gaagttgatt tttctgactt tagtgaacaa ctactcttta tttctatatt gacgcttgta
    10501 gcgattttga ccaaattgct tggaggttat tttggtgctc agattgccgg tttttctcaa
    10561 catagttcat tgatggtcgg tgctggtatg atttcgcgtg gagaaatggc tttgatcatt
    10621 ttacaaattg gacagcaaag tcaattgatc gaaccacagt actattcacc tttagttatc
    10681 gtgattttat taagtacact tatttctcca cttgttctca aatattttac aaaaaaagtt
    10741 tatgaagtgt gagtaaatca aaaagaagtt gtgaaagaga catcgtgaaa accgtttatt
    10801 tgaaaaaagg actgtgacga taacgccaca gtccttttac tgtacttaaa aagcaatttt
    10861 ttattgataa ttttggttga ggtattgcaa tagttccgat gctcgtttgg ttgctgaatc
    10921 atcatcttct ttattttcca acatgctgac catcatataa ccttggttat caagattgaa
    10981 agcaaacaag aaactgttct cttgcccctt tacatcctgt ttttctttga tttccgctgt
    11041 accagttttc gctgccaacg gaatccctaa agcagaaaga gaatgtgctg taccatttac
    11101 atcttgcaca acttctctca gatctggcac gatcgtttgt actgctgttt cgctgatgac
    11161 attcttctta tcttttgtct ctttatctgc aatcaattta ggatagacaa gtgtgccatt
    11221 gttggtaaaa acagaataca ttgctgcttg ctggatagga ttaattagaa gttcgccctg
    11281 tccatatcca gtatcagcta gcaagatatc tgagttaaag ctatcttcat tagaaatttg
    11341 tgctggattc atactaattg gcaaatcaag gtcttcacca aaaatgaatt tatctaagcc
    11401 tgtacgaaat tttttctcac ccattttcaa cgtttcttgt gccgcatata tattatcgga
    11461 atagatcaaa gcagttttta agtctacttg tgatacatca ctactgacac gcgttgtttt
    11521 aaaagatccc caagaactgt ctttttgcca tttaagcccg ttgatcgtca acacttcatt
    11581 tggatcgata gtgccgttgt cgagaccgat tgctgctgta attattttaa acgtagatcc
    11641 aggagcataa cctgtcgcaa atcggctgat gaatggatgt tcaggatttt cttcataagc
    11701 tttgtaatct tcttgtgaga tcccgtttgt cattttgttt ggatcatagc ttggagagct
    11761 agcaagcgca agaagatcac cggtttttgg cgttgtcgca acagttgatc cagcttttcc
    11821 tcctagactg tcaaaagctg ttttttgtgc cttcgcatcg attgtcaatt tgatatcttt
    11881 gccattttga acttcatgtt cgatcagaac ttttttctct acaccgtctg catctgtaat
    11941 gcttaatttc ccacctgttg tcccacgaag atccttatca aaagccatct ccaaacctga
    12001 gcgaccgatt tttccattac tgcttaattc tggattctta tcaatatctt ctgctgtgat
    12061 atctcccacg taaccaatca gctgagcagc tgcttcaccc aaaggataat atctgccgtc
    12121 tacttcttga atggtagctc cagctggaag ttctggcgtt gctccatcaa tgattttcaa
    12181 tgggacaaag taatcgggtt gtacccagct ttgtgaaatc gcctgattga tagcatcttc
    12241 tgttaagtcg aaggaggaag caatcgcttt gatattggct gttttttcgc ctccatctcc
    12301 aagtttactt ggaacgactc ctaattgttt tagtttgcct gttgttgcta atggttcacc
    12361 atttcgatct ataatattcc ctcttgctgc ttcttgtgtg gtcaggctta ctttgtcatt
    12421 tccttccatt tctggaaaaa ccaagttggg ttgccagttg atcgtggttt gaccatcatt
    12481 tctgtctaat gttcctttat aagaaagatc tttcaattca cctaatgagg tattcatctt
    12541 tgctttatat gaaaaactgt aagtagaatc gtctttttta tctactttta gattcgatat
    12601 ctgaagtcct ttgacatcag cagcaccgta tatattctgg tattgatcta agatctcttt
    12661 ttcagataat gcacttttat ttgccgcctt ttttgatgtc atttctgcag ctttgttata
    12721 atctcccttg ttcaaagctt ggacaaattg ctcaactgtc ttttctccag cttctactgc
    12781 ttgggtttct tgatagtgct ggtagtaaaa ataaccgcca cccgcagctg ctattaagat
    12841 cactgcgccg gcaatataag cgcctgttcg atttttgccg tgcttgtcac ttcttttcat
    12901 tttttcctcc ttctcttgct tgtcattcct ttatttcact atttatacct gtttattcta
    12961 acatatgtct ttgaggaaat ccataccaca atccagaagc acactttctg gaaggttcgt
    13021 ttggatacta ccaagtaatt tcccaaccgc tttgggtagc ttgaatattg aagttacgtc
    13081 tttcagctgc tgagagatag ctttgatcac ttgctgctgc cttctgatcc tcccgaagtc
    13141 tccttctttg tcttcccgaa atcgagcata ctggagtaaa gtattaccat ccatcacttg
    13201 ctctcctttg tttatatata cgccatctaa atctagagat ttttctgcat cgatcttgac
    13261 tccaccagga aacagttcat taacgcaatc gataaaatct tgaaatgtga tacttgcata
    13321 atagggagct tcaaatttga aattttcctt taatgtttga tttaataaat caactccccc
    13381 ataagagtaa gctgcattta tcttattcat tccgtaacct ggaatatcga catatgaatc
    13441 tctcatgata gagatcagtt ttgcttgttg tgttttctgg ttgtattgtg caaccagaag
    13501 tgaatccgag cgacctccac caatcgatct tttttcaccg catctcctcc tcacacagca
    13561 atccacttgt caggtgagga gcgagtttgt ctcatcaaat aagtcagtca caaaaatata
    13621 aagaaaaggt cagaaacctt ttcgaacaag aattctgacc tagcatcatc tcatcgtccg
    13681 agttttagca ctatacaacg taaagaaaac attcttagct attggaaaaa gccttaatcc
    13741 aacagtttct gttttaccca ttggcgactt tcgacgtttc tgggcaacag cctatgtttg
    13801 tataggagcc tcacctaaca aggttattac tatattctat ttacgagttc atgatagact
    13861 tttcctaatg attaggcaat ggaatttgaa atgaaatagt gaaatttaac aatctttaat
    13921 attttttaga aaaaaataac aaataagttg aaagaatccc gttgcaaatt ctttcagttg
    13981 caccaggatt ctttgataat tttttagttt tatgtgtttt tttatatgtt ttttcacttt
    14041 tcggaataaa tatttatttg ttaaagtaat taaacaatcc gctaatcgtt tgatctggca
    14101 tttctatttc cttaccatct aaagtaacag tagtaacgac aaaatggtct tgcaatggcg
    14161 gaacttctaa gtactgccct tgcagaaaat cattcactgt ttcatattct tttttcattt
    14221 cttttgaatc tgtcgatggt ttatagctta tttttaacat aactagtgct cctttttatt
    14281 cgggacgtgc aatcgtgaaa atttctccta ttgctgcata atcatttcct gccaaacggc
    14341 ttacagcatt caattttctt ggatcgatcc ttcctgtctt atcatcatag actgtttcat
    14401 ttaaatgata tttctgtact tctagcaaaa ataaatcagc tgttacttta tttttattcc
    14461 gtatctcgac atgttcatat aaggatgttt caaaacgaac ttttgcttct gtcaatgctg
    14521 gtactgaaat gattactgaa tcagttaaag tcaagtcagt caggtacagt tcgctttcct
    14581 cattaggcag attggctgct gttttattta cttcttctac gttgtcctca tcaaggatat
    14641 ggataacagc ttctcttgtc tcaagaatat tctttgctgt gtcttttact gcatcttgtt
    14701 ttctctggat cgccaaggat aagatgggtc gattcgaact gacgatattg aagtaactga
    14761 agggtgcgat attcactata tcttttgctg atttagtcgt tactactgct actggtctag
    14821 ggatgactga accaatcagc aacttgtaat tttcacgttc tgtcaattct gaaggactcc
    14881 gttcaaacat ggtttcgcct tctttctata aaattcgtta aaaagcagat cttgaagaca
    14941 aaatgaaaag taatgctttt ttcatcttct tagaatctgc tttaaactat ctatcaattt
    15001 ttcttttttg tcaagaatct gtgccgtgcg tgttttttag ttatttgctt tacttgcggc
    15061 ttcgacatat cttttagtga tcaattgcgg acgggtgata atagatccaa ctacgactga
    15121 aaaagcacct aattcatatg ctttctctac ttgttcagga gagtcagtat gtccttccat
    15181 aattactggg atatcgactt gttctatgat ttgattcaat aaatcaaaat caggtgtctg
    15241 cgtctttcgt gaatattctg tatagcctgc taaagtcgta gacaaaagat caaaaccatc
    15301 ttcttgcgct tgaagcgctt cctcaaaagt cgaaatatct gccatagcta accgaccgtg
    15361 ttcgtgaatc atttggacaa gttcagcagt tgttttttct gtcggacgtt tacgagaagt
    15421 tgcatccagt gcaatgattt ctgcttgcgt ctccaaaagt tcttccactt ctttttctgt
    15481 cgcagtaata aacacttctg acccatcgta atctcttttg ataatcccta ttaccggtaa
    15541 atcaacgatt cgcttgattt cattgatatc ttctgtcgta tttgctcgaa taccaactgc
    15601 tccgccttct tttgcagcca aagccattct ccccataata aaagaactgt gtagcggctc
    15661 gttttccaaa gcttgacaag aaacgacgac tccgcctttg atttgttcca gtgtatccac
    15721 aatatctcct ccagtaatgt tatataaaaa tacagcttca tataaaaatg taaccctttt
    15781 ctattcagga gacaataatg agctatttct ttcagcagac ctttcttact aaaagaaaat
    15841 tacttcaaat tcttcgtata cgaccagctt ctcttctgta aattctatgt tcaacgcttc
    15901 tttataagca tgtttaaaaa actcagcatg gactttccgc caataagcta atgtccgatc
    15961 cccttctcct tctttccaag catgctcttt cgtgacttca ctaaaaggca agatggtcac
    16021 atttatattt ttgatgatac aaactggttc attttttcca ttcaggacca cgtctaattg
    16081 accaactttt ggtaaaggct cattctcttg tttatacaga tcgtaaccac ttgatgtacc
    16141 agtctttatc ttttttacaa ctagttcgct caactcgtct gccatttttt ctgtatcccc
    16201 aaatgaaaag aaacctaatt tttctgattg ataactagga tatttttctt ggaacttttt
    16261 gacatattct tctacttgct gatacataac atccacccct ttgcttttta taatgaaaaa
    16321 accatccaga tactagtata aacaattatc tggatggtga gcataatgaa ttattgaata
    16381 ttcaagacac gcagactatc ttcaacgatt tgttttggtg ttaaatgata acgttcataa
    16441 agctgcccta caggaacgct atccgtgaat tctttctctg caccaaaatt taatacgttc
    16501 attgaagaat caccataata acgactgata ttttcgccaa atcctcctga caaactacca
    16561 tcttctaatg tgatgactaa ttcgtgtgtt tcttttaatt tatctaatgt ttctacgtca
    16621 ttttctgtga tcgttcctgg attgatcaac gttgcttcga ttcctttttt tctaaaagct
    16681 tttactgttt cttttcctaa ttcaagaaaa ccgcctaaag ctaaaattgc tacttttcct
    16741 ccattattta ctgtttgata ggctggaata ctataatcat ttgcgatagc tgtaccatgt
    16801 tctacaccat gtgtaggaat tcggattgct acaggctgcg ctgtttgttc gaacgcccaa
    16861 tctagcatag agatcatatc ctcttttgtt gctggagcaa gatatttgat gttcgggata
    16921 cttgataagt aaggaatgtc aaatgttcct tggtgtgttc ggtcactagc tgagatcgtt
    16981 ccgccaccga ctaaaataat cactggattc tcgttgatcg ctaaatcatg tgataattga
    17041 tcatatgccc gttgcaagaa agtacttgat tggaagacga ctggacgtac tccctgagaa
    17101 gccaaagcag aagcgaaagt gatagagtgc tgttcagcga tccccacgtc aaaataatga
    17161 tctggatgtt tttcttggaa tgttttcagt ccaaaggcgc ctggtatcgc tgcattgact
    17221 gcggcaatag gagctgtact tgtttgcatt ttcttatcca aataatccat gatgatcata
    17281 ttaaatgttt ctccagaagt tacttgcttt gcctccccgg tggacagatc aaatggtaca
    17341 tgccaatgat atttcatttt attatcgatt gccggctgat aaccgcgacc tttttccgta
    17401 tggacgtgaa gtacaatcgg atgatcaata tcttttactt cttcaaataa acggatcacc
    17461 gtttccagat cgtttccatt atccacgtaa cgataatcaa atcccattgc tttaaatagg
    17521 ttattgctgc ttttaccgtt ggtttgacga agctcagcta agtttttata taaactgcca
    17581 tgattctcat caatagacat ctcattatcg tttactaata cgattagatt ggattgtaaa
    17641 acaccaccat tgttcaatcc ttcaaaagct aagccaccac ttagggagcc gtcaccgata
    17701 aatgctacca catttcctga ttctttcttc agatctctcg ctttagctaa acctgttgct
    17761 aagctgatag atgtagatgt gtgccccact gtaaaatagt catgtttact ttcatgttgg
    17821 ttggaatatc cgctcacttc acggtaatgt cctttttcaa aaccattttt tcgaccagtt
    17881 aaaagcttat gaccataaga ctgatgggaa acatcccaaa cgattttatc ctttggtgaa
    17941 tcaaatacat aatgaaacgc aatcgttaat tctactacac ctagattcgg accaacgtgc
    18001 ccacctacat cgctgtcctt ttgcaagatt ttttggcgca tctcttctgc caactgtttc
    18061 atttcctcaa cagataattt tttgacatct gcggggctat tgattgcatc caatacatgt
    18121 aatactgtac tcataataca ttctctcctc cgtactatct tcttttaatg aaaatagatt
    18181 tgttacactt tatctattct actacttaga gttgactcta agtcaatagc attttaaaaa
    18241 aatttcagag aaagcttgtt atataagtag atcttcgcac gaatcaggac tcaaataaaa
    18301 accttctaca gacaattaaa aagctttgta gaaggtctcc taattaaggt ctcatcaaga
    18361 aatatgtttt tttaataggt gaagttcctg agcaatatga tacaaatgga tgtttgcacc
    18421 ttttaatgag attggataga tctttgtctt ttcagtttct aatccatttt gatagctagc
    18481 agcatcgcac aaaaccactc tttttttcgc acatagacat tcatcaggca attcttctaa
    18541 tgaacgaaat aaaataggat ttatttcagc gatgccttct tccgcataca aatgaaattg
    18601 ggttcgataa aaaaattgtt tgactgaact ttctgaaaca acaaaaattt ctatttcctg
    18661 tctccccgct tgttcactgt tgatcaaaga aagacttttg acgaattttc gaatgaaatt
    18721 ttcattccat cttagttgga tttgattttt ctttttccac tcttccaata tttctacaat
    18781 ttttccgtac agaagttgtt ctttttctga tagaagatag atcctttccg gtaaaaatac
    18841 ttgtccatct gcccacaacg tacggacaaa ttgtaaaaag gattgcttga aaataaaatt
    18901 ttctgcatcg tcgatttcag cgatactaat aatctgtaaa atcaattcat tcagtgatag
    18961 gtcattgaga acaaaatttt tataaacaac ttcgaaatct tttcttaaca gctccatgtt
    19021 ctcattggta taatttctac tgttgaacaa agaaaaaata taatagacat cttcttcttg
    19081 taagtctaga attccttttt gctcttccaa tccttgacag actaattgaa ataacggctt
    19141 cgtttctgcg cttttcttat cttcttcttt cagtccaagg ggaaatgcct ttcttccgat
    19201 tgcgatctct attcctcgta tgataaagga cttttcatct tccgaatatc ttcttttaaa
    19261 aaaatgagtc tctacataat caatcaactc gttacaagct tcttctacat acggattttt
    19321 attttcatag ccttcccaat tgacataacg tactaaagct agcaagatgt tccgcgagtc
    19381 aaattctggg ataatgattt ctttattttt caaataatca ttttctttaa aaaaatccaa
    19441 tacgattttt ttgattgtat atactttgct caaagaaata aattcaagat cagccagttc
    19501 tgtactcgtg aatataccat taaaataata acttaaaaac tttaaaaaat aagattgacc
    19561 gtacagaaac tgtgtgagct cagaaagagc aataccatgt ttgaatacga gtttatactt
    19621 atgcttctct cttacttgtt cttttataca atattctaga cactcggcta gactttctag
    19681 atcgtgaagg atcgttggtg tagtcaccct tagagaaata gctgtttgtt cgatgtctat
    19741 ttcttggaat tcaaataatt gttcacacag atacactttt ctaaatatat ttcgctcgat
    19801 gtaattttcc agcattcctg atacacgcct ccagtttttc ttatgatttt atttaatcat
    19861 atctgtaaat cgtatttttg tttccttttt atttagaaaa gccctttcaa aaaccaataa
    19921 aaaaagaaac tgggacaaaa gtcaaacaag ctcacaattt cagcctcatt gtaggtagct
    19981 ttgagccgtt tggactttta ccccagcctc tttcaagaat ggttattcaa agatgtcagc
    20041 gtctagttct ttgactacat ctttcaacaa ttggatacca gctgcgctgt taccaaatgg
    20101 atctagtgcc gggctgaaga cacctatgcc gtaacgattt ggcacagctg ccatcaaccc
    20161 gccgcctaca ccgctttttg caggtacacc aacatggacg gaaaactctc ctgattcatc
    20221 atacaaacca gcagttgtca taattgattt gacgattgta gcactttctt cagtaatgat
    20281 ttgtttgcca ttccaaggag caattccttt atttgccagc acagcagcta attttgctat
    20341 gcctgtagcg gtgacattga gggagcattg tctgaaataa gcatcgagta tctccggtac
    20401 gtctccttcg atcatttgat tacctttcat atagtaagca agagagcgat tgatatcacc
    20461 tgttcttgat tcagaatgat agatttcttc attcaatgtg atattaggat catcacagat
    20521 ctctttcata aatgacaaga ttcgctcaaa tttttcatct gcatctttac cggcaatcaa
    20581 cgaggttgtt gcaatcgctc ctgcattaac aaaaggattc attggatgtt tgcgatgatt
    20641 gatttccata ttcatgatag aattgaaggc aaaaccggtt ggttccgtgt tgattgtttg
    20701 aaagactttt tctattccgt tgtctttgat tgctaataac aatacgggaa cttttgacat
    20761 gctttcgatg gcaaagcgca catcggcgtc tcctccctca atcagttgtt ttgtatccaa
    20821 atcataaatc gccataccta attgtttggg atccacttca gctaatgctg gaatgtaatc
    20881 cgccacttgt cctgtttgat aatagtggaa attttttcca atcaattgtt ctaattcttt
    20941 tacttccatt tgaataacca gctttcattt tttatttttc tgcatgctgg aatttatgac
    21001 ggcatgcgta gatcgtaaat ggaataacta agacgataag ataagaaaca atcagaatcg
    21061 tttcataaat cccatgctgt ccagctgcga tactagcagg tggaacaaat gagatacaaa
    21121 aagcaaaaag tgaagtcaaa agacctaaac aagcaacgat tacttttaca acttttccgc
    21181 ctggaacctg ataggctctt tcaagactgt catctttaaa aaccagcttc aaatagcccg
    21241 cgaaaaacat aaagtacaac atcaggtaga ttacaacagt caatgacatc gctgcaagaa
    21301 atgaaagatt attccccccg ccgcttaatg taaagacggc tgcccagatt gttgcaacga
    21361 tcccatttgc gatcatcaaa tgagcaggaa caccgtgtgt gttttctttt gcaaaatatt
    21421 caggaagtaa tccttcttct gctgctacgt gaagtgcttt tgaaggagag gttacccaag
    21481 aagcaatttc tccgatgaca cctaaacaaa caagaatcgc aagaattttc gcaatccatg
    21541 caagatttgg atcaatgtgt cttaatagga attctaacga ttgaatcacc cctgtattaa
    21601 gagacaaacc atgttgaggt actgtagtag cgacggtcaa tccgccaaaa gtatctaaaa
    21661 tgattgccag aataaccaat acaaacattg ctaatggata atccttttta gggttttcca
    21721 tttcatttgc atgactggca gatgtttcta cgcccatata tgctaaaata aaggaaacaa
    21781 aaacaaccag cgtaggcaat ttcgaaaaat caggaatcaa tgttttcata gacagtatcg
    21841 tttgtagtgg agcgcctgaa gcgacataat ggatccctaa gccaaacaag actaatgctg
    21901 gcaacaagat accagctaca aaaccgatcc gagcaatcaa tgaagtgtat ttggttcctt
    21961 ttaactgtga gagtaaaata gcccagaaaa taatcaatag gattgccaat ttcagatatt
    22021 tattgttttg tatagcacca atacccgttg cataggataa tgcaccagtt aagaaataca
    22081 gcatggtcaa gtagccaact gttatctcaa accattggaa aaagattgcg gcaaaaccga
    22141 atcgttttcc caatgtgcga ctgacccaag tatagacacc gcctttttcc catcctttca
    22201 ccgttgccat ctctgctgcg cacaatgcca cagggataaa ccatagcaaa ccgcctaata
    22261 acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt gggatattca tataatgaaa
    22321 cgaccattga gatggtcata gccaaaaagc caaagaacgt taattttttt gcaggaattt
    22381 tcgttttgct ttgttgcatg aatcttcctc ttttctattt gaatttgttt caattgagtt
    22441 tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc ttcttttgta gtagtgatcg
    22501 ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag cacataggca tacacggcag
    22561 taatgatgac accaatcact tgaatcccta agaagtacca gctgcttgga ttcgttagta
    22621 aatggctgtt agatgcaaag atccctatca gaatcgtacc tgtgaatccg cccatgccat
    22681 gaacacccca cacatcaagt gcatcatccc attgtttctt tttacgaaaa tcaacagcag
    22741 catgacagac gatacccgca atgatcccaa tcagcatggc actttttgct tctacataac
    22801 cggcacaagg cgtaatggct gccaatccag caacggatcc tgttagtacg tcgacaaaat
    22861 caaagccatt tccttttact tttgcaatga tcagccaagt aatcatcgca aatgcaagag
    22921 cgatgaatgt tgaaccaaat gcattgactg cttgttcatc tgctcgaagt gcaccaccgg
    22981 aattaaaacc gaaccagcca aaccaaagca gtcctgtacc gatagcagct gccataagac
    23041 tgctatgctg acttctttct cctaaaggaa tctttctttg accaagtgtg aggatacatg
    23101 ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc accagcgaaa tctctaaatc
    23161 ccatctgatc caaaaatccg ccaccccata tccagtggca aacagggaaa taaatcagca
    23221 gattccaaaa aataacaagt ataatatagc cttttaaatt caatctccct gcaaaagctc
    23281 ctgtcatcaa aggaacggta atcacgataa acatcagttg gtaaagaaag aacattaaaa
    23341 atggaatcgt tgcaccatga aacatgttgg ggaagcttgt aacatgtctc atcaaaaaga
    23401 aatctgccgg gtttccaata acaccgccga tatcccgtcc aaaggccaat ccgaatccac
    23461 caaataccca cataatcgtt gttatcccaa cggagagaaa agattgagac atgatcgtta
    23521 gtatattatt cttccgtgct aatccgccgt aaaaaaaagc tagaccagga gtcatcaggc
    23581 aaaccattgc tgtacataaa atcatgaatg ctgtatctcc tgtattgata ggtaatgtat
    23641 tcattttttt cgcctccttt ctacgattac gagtataaaa gaaggtgtag ttgttttttt
    23701 cttctgtttt tttgaattaa tacttgcatt tataaaagac agacaattaa taaaagcttg
    23761 atgtgttgtg ctctttttcg tttttcacac aaataaagac aaaaaataaa cggtcttctt
    23821 tttatagaaa accgtttatt ttcgtctttt tttattttta aagatatttt ttcataagga
    23881 ataaagaata cttcatataa aatatataaa aaagaaccgc agcgagtgtc gctacggttc
    23941 tcaaatttgt tcttcatttc aacataagtt tttcttcaat ttgcttataa tttacgtgca
    24001 aagaatgaaa cgactaatac taaaatgatt gcaccgatca atgatggaat gatagccatt
    24061 cctgcaattg ttggtcccca agttcctagt aaagcttgtc caattgctga acctactaaa
    24121 ccagcaatga tgtttgcgat gatccccatt gatgatcctt tattcgtgat tgctcctgca
    24181 attgctccaa tgataccgcc gacgattaat gcccaaataa atcccatttt aaattcctcc
    24241 taactgactt cttacttctt attgtacacg tggttcgtta ttttctttga ctttttgtgc
    24301 acctttgcct acagcagatt ttgctttatc tgtttggcgt gaagcaaatt gtcctgtgga
    24361 ttttgctgct tctgttactt tgtcttgtac tgtttcttga tctttttggt attcttcttg
    24421 tgttttgatg tctgcaacat tcacattcac ttcgataaca tctaaatgtg tcatattgtt
    24481 tacttcagta ctgatcaatt ctttgatttg gttgtaaata tcttcgatgt ctttaccgta
    24541 ttctacgatc acatctagat ccacagctac ttgtttctta cctacttctg tattgatacc
    24601 tgctgttacg ttatctgtgt tgactaattt ttcagccata tttgagaaaa atccgccatc
    24661 taatgtcaat agaccatcta catctttcaa tgcataaccg atgatttttt ggatgacttt
    24721 gtcttcataa gtcaattcac cattcatcat gcggttttct cctttttcat ttgccaaaac
    24781 tgatccattt gttgtattca tgtttttgtt ttccatcttt gtttcctcct atgatttaaa
    24841 aaattgatca actattcctg actgtttcaa ataaaaaccg attcctgcgc caattgctgt
    24901 catgattatg atcaacaatg tcttgaaaaa tccaatcgtt aaccataaaa cagctagcag
    24961 tagtccaaga cccccaaaga taatagggag cttgtatgct gaaaatagtt ctttcattat
    25021 ttggccctcc tattccactc gagagtgctt gtagtctctt gtatcttctt gttcttcata
    25081 accgctgtat gtgactgcca ctttgaccgt ttcttttgtt ccgagtactt tagttacttc
    25141 ttcttcgata tcttgcatca atacacctgt tttccctact aatgaagaag ttcgtttcaa
    25201 gtctccgttg acatggatat ggattcgatt tttcgttgaa cgtatccgta ctttaggtga
    25261 atgaatgaac tcttcttcgt gtaaatggga acgtaccaac ccttcgatcg cttttttgtc
    25321 gattgttagt ttgccatctt cccgtttcat aacgaaagtt cctcttgact ttggatagaa
    25381 aagaacgact agcaagatga taatagcaag aacggcaaat actacagctg cccagaaaat
    25441 atattgttgc atgtagtaat tcgttaaagt aaagaagcga aatcgttcca attgttcagg
    25501 cataatatac ggcgcattga tcaacgcaat cgctagaaat actgaaagta gcagtaaact
    25561 catgataaca ccgattattt ttaacccctt attcattttc cacctcctat aaacttattt
    25621 ttaccctgta aataactcta ccgacaatca cagtatagat agcgaacagt tagcatagca
    25681 tcagtaaata aagcgctacc aaacattgct ggatcataac ggcgttttat tgccagtctg
    25741 atactgaaat aacaattgct tacttattct ctatttactt ttgattgctg gctatactta
    25801 aaggatatta ttcaagatta aaaattgcaa agattatgct cagttattat tacaagattc
    25861 tatttattt
//
LOCUS       DACEDO010000042        25677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEDO010000042 DACEDO010000000
VERSION     DACEDO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.042"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            172..516
                     /locus_tag="IWO52_001736"
     CDS             172..516
                     /locus_tag="IWO52_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0356004.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="IWO52_001737"
                     /pseudo
     CDS             517..936
                     /locus_tag="IWO52_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="IWO52_001738"
     CDS             complement(1028..1378)
                     /locus_tag="IWO52_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356005.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="IWO52_001739"
     CDS             complement(1344..2675)
                     /locus_tag="IWO52_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0356006.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="IWO52_001740"
     CDS             2931..3497
                     /locus_tag="IWO52_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0356007.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="IWO52_001741"
     tRNA            3642..3729
                     /locus_tag="IWO52_001741"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /locus_tag="IWO52_001742"
     CDS             4063..5037
                     /locus_tag="IWO52_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0356008.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="IWO52_001743"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="IWO52_001743"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0356009.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="IWO52_001744"
     CDS             complement(7452..7898)
                     /locus_tag="IWO52_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0356010.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="IWO52_001745"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="IWO52_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0356011.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="IWO52_001746"
     CDS             8363..8881
                     /locus_tag="IWO52_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0356012.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="IWO52_001747"
     CDS             complement(8958..9914)
                     /locus_tag="IWO52_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0356013.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="IWO52_001748"
     CDS             10080..10886
                     /locus_tag="IWO52_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356014.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="IWO52_001749"
     CDS             10883..11872
                     /locus_tag="IWO52_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0356015.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="IWO52_001750"
     CDS             11869..12873
                     /locus_tag="IWO52_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0356016.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="IWO52_001751"
     CDS             13005..13547
                     /locus_tag="IWO52_001751"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0356017.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="IWO52_001752"
     CDS             13567..14265
                     /locus_tag="IWO52_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0356018.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="IWO52_001753"
     CDS             14286..14498
                     /locus_tag="IWO52_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0356019.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="IWO52_001754"
     CDS             14498..15460
                     /locus_tag="IWO52_001754"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0356020.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="IWO52_001755"
     CDS             15478..15873
                     /locus_tag="IWO52_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0356021.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="IWO52_001756"
     CDS             16036..16401
                     /locus_tag="IWO52_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0356022.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="IWO52_001757"
     CDS             16398..18209
                     /locus_tag="IWO52_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0356023.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="IWO52_001758"
     CDS             complement(18271..18438)
                     /locus_tag="IWO52_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0356024.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18686..19675
                     /gene="galE"
                     /locus_tag="IWO52_001759"
     CDS             18686..19675
                     /gene="galE"
                     /locus_tag="IWO52_001759"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0356025.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19687..21171
                     /gene="galT"
                     /locus_tag="IWO52_001760"
     CDS             19687..21171
                     /gene="galT"
                     /locus_tag="IWO52_001760"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0356026.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21195..22175
                     /locus_tag="IWO52_001761"
     CDS             21195..22175
                     /locus_tag="IWO52_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0356027.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22264..23088
                     /gene="tsaB"
                     /locus_tag="IWO52_001762"
     CDS             22264..23088
                     /gene="tsaB"
                     /locus_tag="IWO52_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0356028.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23072..23614
                     /gene="rimI"
                     /locus_tag="IWO52_001763"
     CDS             23072..23614
                     /gene="rimI"
                     /locus_tag="IWO52_001763"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0356029.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23621..24085
                     /gene="rimI"
                     /locus_tag="IWO52_001764"
     CDS             23621..24085
                     /gene="rimI"
                     /locus_tag="IWO52_001764"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0356030.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24082..25098
                     /gene="tsaD"
                     /locus_tag="IWO52_001765"
     CDS             24082..25098
                     /gene="tsaD"
                     /locus_tag="IWO52_001765"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0356031.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8330 a         4398 c         5378 g         7571 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaattg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgcat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaaag
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat tttactttgt
    18601 ttgttttcac cataaaagta aacttcgttt tttaaattat atagaatcaa caaacacaat
    18661 tttactaatc aaaggagtgt tttttatgac aattttagta ctaggcggag ccggttatat
    18721 cggttcgcac gctgtagacc agctagttca aaaggggtat caagtagctg tggtagataa
    18781 tttgctcaca ggtcacaaac aagcagtaca tccagatgct catttttatg aaggagacat
    18841 ccgggataaa gaatttctac gttccgtatt tgaaaaggaa ccgattgaag gagtaatcca
    18901 ttttgctgca agctctttag taggagaatc tgtcgaaaaa ccattgatgt atttcaacaa
    18961 caacgtatac ggtatgcaaa tcttacttga agtcatgcac gagttcaacg tgaataaaat
    19021 cgtattctct tctactgcag caacttatgg agagccgaaa gagtctccaa tcacagaaga
    19081 tacaccagct aatcctaaaa atccgtatgg tgaaagtaaa ttgatgatgg aaaaaatgat
    19141 gaagtggtgt gaccaagcat atggcatgcg ctatgtcgct ttgcgctact tcaatgtagc
    19201 cggagcaaaa gcagatgcat ctatcggaga agatcataca ccagaaacgc atcttgtccc
    19261 aatcatttta caagtagctc ttggtcaaag aaaagccttg gctgtttatg gagacgatta
    19321 tgatacacca gatggaacat gtatcagaga ttatgttcaa gtagaagatt tgattgctgc
    19381 acatatttta gctttggaat acttgaaaga aggaaatgaa agcaacttct tcaatttagg
    19441 cagcagcaaa ggatactcag tcaaggaaat gctagaagca gctagagaag tgacaggaaa
    19501 agaaattcca gcagaaatag caccaagaag agctggagat cctagccgat tagtcgcttc
    19561 aagtgaaaaa gcgagagaga ttctgggctg gaaacctgaa tacacagata tcaaagcgat
    19621 tatcaaaaca gcatgggatt ggcatgtcag ccatccaaat ggttatgaag aataggggaa
    19681 tgagatatgt caatcagtca aacgatttgt gattttgcca cacttgcgat cgaagctggc
    19741 ggatggatgg aaatggaccg cttatattta cagaatcgtc ttttagggat ggtaggagaa
    19801 gatgctctag aaagcattga agtaagacca gttaaggaag aatctgtaga cttattggat
    19861 cagcttgtcg ctaaggcaag agaaaatggt gttctttcag aattgccagc agaacaagag
    19921 atttttgaag cgcaattaat ggattttctc acaccgccac cttcagtcgt caatgcattc
    19981 tttgcacagc actattcgaa aagcccagag gaagcaactg agtatttctt taatttaagt
    20041 aagaaaaatg actacatcaa aacaagagcc atcgcaaaaa acatcaaatt tccggcagag
    20101 tcggagtatg gtgaactgga aatcacaatc aatttatcta agccagaaaa agatccaaaa
    20161 caaatcgcag cagaacgcga ggcggtaaaa gtagactatc ctacctgcat gctttgcatg
    20221 gaaaacgaag gatatagagg tcgcttgaac tatccagccc gcaccaatca ccggattatt
    20281 cgaatgaatc tagacggtga aagctgggga ttccaatatt ccccttatgc ttattacaat
    20341 gaacattcga tcattttatc agaacaacac cgcccgatga aaatcacaaa ggaaacattt
    20401 gctagactgc ttcggattac tgaagtcttg cctcattact ttgttggttc gaatgctgac
    20461 ttaccaattg ttggaggatc gattctttct catgatcatt atcaggcagg acgtcatgaa
    20521 tttcctatgg caaaagcacc gatagaaaaa cttatcaaac tagaagatta tccgcaagtc
    20581 atcgcaggag tcgtgaaatg gccgatgtct gtgatccgtt tgcaatcaac agagaaaaac
    20641 ttgcttattg aagcagctaa ctcagtattg gaaaaatgga aagcatattc ggatgaaaca
    20701 gtatctatca gagctttttc agcagatggt acaccacacc atacgattac gccaattgca
    20761 cgaagaaggg acaattactt tgagttggac cttgtccttc gagacaacaa tgtttcagaa
    20821 gaacatcctg atgggatttt tcatccacat aaagatgtcc aacatatcaa aaaggagaac
    20881 attgggttga tcgaagtaat ggggcttgct gttttaccac ctcgtttgaa accagaacta
    20941 gtagaagtag agcatttctt attaggagaa gaaaaccaga tggctgaata tcatcgtccg
    21001 tgggcagaac aattaaaaaa agaacatcct gatttgacga aagatacggt caccgatgtc
    21061 gtacaaaaat ctgtaggtca ggtttttgca cgtgtactgg aagacgctgg agtattcaag
    21121 cgagatgaaa aaggacaggc agcgtttgaa cgatttactg cgattttata aacgtaaaaa
    21181 aggaggagag acaaatggca acaattaaag acattgcgca gttggcagga gtttctcctg
    21241 ctactgtttc aagagttctc aactatgatc ctgatctttc cgttggctta gccacaaagc
    21301 aaaaaatttt tgaagcagca gaacgcttaa attatacgaa acatcgtaaa aatgcagcaa
    21361 atactaagca aacgcttcgc ctgatccagt ggtacgacga tacggaagag cttgaagatc
    21421 tttattattt atctattcgc ctaggaatcg aaaaaaaggc agaagaagca aacgttactt
    21481 tattgaaaga aacgtgggat tccattagtg atcttccggc agatggaacg atcgcactag
    21541 gaaaattcga cgaagctcag ttattagaaa tgaaaggatc ccaaaatgcc ttgcttttgg
    21601 tagattctga cggcacttct caaggaatcg actctctagt tgtcgacttt aagtcaagtg
    21661 ttcaaaaagt cattgaacac tttgtctccc ataaagcgca acatatcgct atgcttgctg
    21721 gaacagaata tacgaagaaa aaccatcagc ggctaaaaga tcccagaatt atggcatttc
    21781 aacaagtttt ggcagaaaaa ggctacgacg aaggtccaat tatcgaagca gatttttctg
    21841 tcgagtcagg atatcaagca gtgaagcagt atttaaaaac gaatccgcat gttcctgatg
    21901 cgctttttgc agctaatgat gcattagcga ttggcgcact gaaagcgatt caagaaaaag
    21961 gtttagtggt accagaagat atttctgtga tgggcttcaa tgatgtcagt gtagcgaaat
    22021 atgtaacgcc ctcgctttct actgtaaaag tttataccga atggatggga gagctagcag
    22081 tggagacgat tcttcaatta agtaattcca aagcaccggt ttcaagaaag attattgtgg
    22141 aaacagagtt gattattcga gaatcaacga aataaaagat ggaaaagctg tgggaaaatg
    22201 gagtgatacc gcagcttttt tgtgtttcag gaaggggcaa aatttgcccg ccagcagata
    22261 atcatgtatt attgtactag ttttgccttt ggtattttca tcctcagaat aaaaaagtat
    22321 gctacaatgg aaggcgagaa tgtcatagca gaaaggatca aatatatgat tacattagga
    22381 atagatactg caaaccaaac tttggcgatc ggtgtggtag aagacgaaca aattcttgga
    22441 caaatacaaa caaatatcaa acgcaaccac agtgtaacac tgatgcctgc aatcgaccaa
    22501 ttgtttgcag atcttcaaat aagtccaaaa gacattgacc gaatcgcggt ttctgatggt
    22561 cccggttctt atacagggct tcgtataggt gtaacgacag caaaaacgat tgcttataca
    22621 ttagataaag agctagtagg agtatctagt ctgaaaacca tcgcggctaa ttgcatcggt
    22681 gtggataata ttattgtgcc catgtttgat gctcgaagaa aaaatgtata cgctggggca
    22741 taccgctgga aagaaaatag attagaaact gtacttgaag atgtacatat ttctgcttct
    22801 gatttgttca cacaactaca aaagctgaat gaagctattt atttcgtcgg gagtgattgt
    22861 cacaaattct tctctgaaat aaaggaaata ttgcctagtg cgatgattaa tacagttcca
    22921 gcatgggata ttccaagtgg tgtaactgtg gctcaattag gtgctttagt tgaaccagtt
    22981 gcagagaaac aagcttttct tcctcgctat ttaaaacgag tagaagcaga agaaaaatgg
    23041 ttggagacgc acacacctgg agacgaaagt tatgttgaaa aaatttagag cgtatgccag
    23101 aagctttttt caagataaaa tgatttatac agaaaaaaca gtcgaacaaa tcgatcgtcg
    23161 ttacgtacta agagaattga ctacagaaga tatcaaagag ctgctggatg tcgaacgaga
    23221 agtctataat ggcgaattgc catggacaaa aagtgccttt atatcagaat tgaagtcacc
    23281 gcttcctcat ctatatctag gtatctttga taatggaaga atgattggtt ttatcggttc
    23341 aagagcgttc ggtttagatt gccatatcac gaatattgcc gttcgaccgg catatcagcg
    23401 taaacgaatc ggttcgctgc ttattgacga aatcgagaac tttgctataa tgaatcgctg
    23461 tgagacgatg tcattagaag tgcgtatgag taatcaagat gcacaaagac tttatcggaa
    23521 attggggttt caagcaacaa cgatcaaaaa aagatattac actgaaacaa aagaagatgc
    23581 gttggatatg ataaaacttt tagaaaatga gtgagattag ttgatttgca gaaaaaaaga
    23641 ttttgatgaa gcgtacttgg cagaatgcgt atggatgatt agcgaaaatg cttatccaca
    23701 tggttcacct tggacgaaaa tgcagttttt ggcagacatc caacagcctc actctgatta
    23761 tctgctttta gctgaagata accagatctg tggctttatt ggatactcga aagtaataga
    23821 tgaagtcgag atcacaaata ttgctgttgc agtttcagaa caaggaaaag ggcatgcacg
    23881 gcatttgtta caattactga ttacggaaca aataaaagat tcgctgcatg tttttttaga
    23941 agtacgtcta tctaatgaag cagcaagaaa actttatgaa tcagaaaagt ttcgtatatt
    24001 aggtaaacgg aaatcatatt atcgcaatcc gacggaagat gcgatcatta tgagcagaaa
    24061 gttaaaaacg gagacgatga aatgaatgaa gaattgattc tggcgattga atcaagttgt
    24121 gatgaaacga gtgtagcggt cgttagaaat ggaacagaga tcttatctaa catcgttgct
    24181 tctcaaataa acagtcataa acgttttggt ggcgtagtac ctgaagtagc tagccgccat
    24241 cacgtagaac aaatcacctt atgcttggaa gatgcactgg tagaagctgg tgtgtcggca
    24301 gaagatttat cggcagtcgc tgtcacttat ggaccaggac ttgtcggctc gttgctgatt
    24361 ggtatttctg cagctaaggc ttttgcttgg gcacaccaat tgccgcttat tcctgtaaac
    24421 catatggcag ggcatatcta tgcagcccgt ctggttaaac cattccagtt tccattgatg
    24481 gcacttcttg ttagcggagg acatacagaa ttagtctata tgcaagaaga tggatcatat
    24541 gaaatcattg gggagacaag agatgacgca gcaggagaag cttatgacaa agttggaaga
    24601 gtattaggtc tttcttaccc aagcggaaaa gaaatcgatc agctagctca tcagggaaaa
    24661 gataattacc attttccgcg agcgatgatc catgaagata attatgattt cagttttagc
    24721 ggattaaaaa gtgcatttat caatttggtg cataatgcac aacagcgtgg agaagatcta
    24781 gacaaaaatg atttagcagc tagctttcaa gcaagtgtga tcgatgtatt gattactaaa
    24841 acacttcgag cttgtcaaaa ttatccagtc aagcaactga ttatagctgg aggtgtagcg
    24901 gccaatcaag ggttgcgtga aggattgcag gatgctttgg caactaaact ccctgaggta
    24961 gaactagtga ttccccctct tcgactttgt ggagataatg ctgcaatgat tggagcagca
    25021 gcacatgtgg aaatgcaaaa aagaaatttt gctagctatc agttgaatgc tgatccaagc
    25081 ttggttttat ctgaataaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    25141 gcccaggaaa aacggcttct catgtttttg acctggctgt tcttattacc gcgtatgcaa
    25201 gcttttgaac gccgtttaat caagagttcg taagcctgtc gtttaactct gattcacaag
    25261 gaacaatttc aaaaaacagc tcctcatgtt tatgaacatt atgacttgtg accgaagagt
    25321 tgacaggtac acaccgttta atcgaaatag ggctgtgaca cgtttttgtc aaagccctat
    25381 tttttacata cttttaatca ttattgttat tttgctattt tttacagctt atttatcatt
    25441 atccgttcct ctgattgccc tcactgagta atcgtttatt gtcgaggaat atcgaataat
    25501 ctagatcgga tgaggacaag acgcttcatc tagttaatct aacagtattt tttgcaaaca
    25561 gtgaatatta cataactgaa acacacatag aaaatctttg agtcttgtcc agtgatctaa
    25621 aactgccgga tctttcagaa gttttaggga ggaaaataag cagtgaccct gatattt
//
LOCUS       DACEDO010000043        24574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEDO010000043 DACEDO010000000
VERSION     DACEDO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.043"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(154..552)
                     /locus_tag="IWO52_001766"
     CDS             complement(154..552)
                     /locus_tag="IWO52_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0356032.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="IWO52_001767"
     CDS             complement(549..1322)
                     /locus_tag="IWO52_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0356033.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="IWO52_001768"
     CDS             complement(1319..2002)
                     /locus_tag="IWO52_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0356034.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="IWO52_001769"
     CDS             complement(2169..3056)
                     /locus_tag="IWO52_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0356035.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="IWO52_001770"
     CDS             complement(3053..3922)
                     /locus_tag="IWO52_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0356036.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="IWO52_001771"
     CDS             complement(4059..5345)
                     /locus_tag="IWO52_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0356037.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="IWO52_001772"
     CDS             5618..6196
                     /locus_tag="IWO52_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0356038.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="IWO52_001773"
     CDS             6196..7125
                     /locus_tag="IWO52_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0356039.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="IWO52_001774"
     CDS             7122..8858
                     /locus_tag="IWO52_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0356040.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="IWO52_001775"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="IWO52_001775"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0356041.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="IWO52_001776"
     CDS             complement(9754..10263)
                     /locus_tag="IWO52_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0356042.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="IWO52_001777"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="IWO52_001777"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0356043.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="IWO52_001778"
     CDS             complement(11564..12655)
                     /locus_tag="IWO52_001778"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0356044.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="IWO52_001779"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="IWO52_001779"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0356045.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="IWO52_001780"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="IWO52_001780"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0356046.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="IWO52_001781"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="IWO52_001781"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0356047.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16618..17592)
                     /locus_tag="IWO52_001782"
     CDS             complement(16618..17592)
                     /locus_tag="IWO52_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0356048.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGKTYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17573..19456)
                     /locus_tag="IWO52_001783"
     CDS             complement(17573..19456)
                     /locus_tag="IWO52_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0356049.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19474..21699)
                     /locus_tag="IWO52_001784"
     CDS             complement(19474..21699)
                     /locus_tag="IWO52_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0356050.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21814..22848
                     /locus_tag="IWO52_001785"
     CDS             21814..22848
                     /locus_tag="IWO52_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0356051.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22915..23928)
                     /locus_tag="IWO52_001786"
     CDS             complement(22915..23928)
                     /locus_tag="IWO52_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0356052.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24006..24194)
                     /locus_tag="IWO52_001787"
     CDS             complement(24006..24194)
                     /locus_tag="IWO52_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0356053.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5339 c         4397 g         7777 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    16561 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    16621 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    16681 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    16741 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    16801 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    16861 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    16921 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    16981 tttataaata ccttcgacac caccagccat ccgatccgga tatgttttcc ctgataggcc
    17041 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    17101 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    17161 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    17221 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    17281 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    17341 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    17401 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    17461 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    17521 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    17581 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    17641 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    17701 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    17761 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    17821 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    17881 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    17941 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    18001 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    18061 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    18121 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    18181 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    18241 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    18301 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    18361 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    18421 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    18481 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    18541 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    18601 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    18661 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    18721 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    18781 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    18841 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    18901 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    18961 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    19021 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    19081 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    19141 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    19201 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    19261 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    19321 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    19381 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    19441 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    19501 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    19561 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    19621 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    19681 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    19741 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    19801 gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    19861 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    19921 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    19981 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    20041 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    20101 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    20161 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    20221 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    20281 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    20341 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    20401 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    20461 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    20521 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    20581 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    20641 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    20701 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    20761 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    20821 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    20881 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    20941 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    21001 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    21061 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    21121 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    21181 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    21241 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    21301 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    21361 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    21421 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    21481 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    21541 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    21601 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    21661 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    21721 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    21781 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    21841 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    21901 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    21961 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    22021 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    22081 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    22141 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    22201 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    22261 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    22321 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    22381 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    22441 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    22501 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    22561 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    22621 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    22681 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    22741 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    22801 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    22861 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    22921 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    22981 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    23041 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    23101 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    23161 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    23221 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    23281 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
    23341 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    23401 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    23461 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    23521 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    23581 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    23641 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    23701 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    23761 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    23821 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    23881 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    23941 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    24001 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    24061 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    24121 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    24181 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    24241 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    24301 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    24361 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    24421 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    24481 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    24541 cttattggca aatcgccaaa ataatgtaga ctaa
//
LOCUS       DACEDO010000044        23360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEDO010000044 DACEDO010000000
VERSION     DACEDO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.044"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IWO52_001788"
     CDS             25..696
                     /locus_tag="IWO52_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0356054.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IWO52_001789"
     CDS             755..1195
                     /locus_tag="IWO52_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356055.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IWO52_001790"
     CDS             1207..1875
                     /locus_tag="IWO52_001790"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0356056.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IWO52_001791"
     CDS             1939..2562
                     /locus_tag="IWO52_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0356057.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IWO52_001792"
     CDS             2618..3004
                     /locus_tag="IWO52_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0356058.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IWO52_001793"
     CDS             3217..4608
                     /locus_tag="IWO52_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0356059.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4955..5602
                     /locus_tag="IWO52_001794"
     CDS             4955..5602
                     /locus_tag="IWO52_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0356060.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            5995..8640
                     /locus_tag="IWO52_001795"
     CDS             5995..8640
                     /locus_tag="IWO52_001795"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0356061.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FYRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            8950..10263
                     /locus_tag="IWO52_001796"
     CDS             8950..10263
                     /locus_tag="IWO52_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0356062.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            10253..10906
                     /locus_tag="IWO52_001797"
     CDS             10253..10906
                     /locus_tag="IWO52_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0356063.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            10961..11653
                     /locus_tag="IWO52_001798"
     CDS             10961..11653
                     /locus_tag="IWO52_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ0356064.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            11803..12003
                     /locus_tag="IWO52_001799"
     CDS             11803..12003
                     /locus_tag="IWO52_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0356065.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(12257..13936)
                     /locus_tag="IWO52_001800"
     CDS             complement(12257..13936)
                     /locus_tag="IWO52_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0356066.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(13938..14150)
                     /locus_tag="IWO52_001801"
     CDS             complement(13938..14150)
                     /locus_tag="IWO52_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0356067.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            14543..16273
                     /gene="efrA"
                     /locus_tag="IWO52_001802"
     CDS             14543..16273
                     /gene="efrA"
                     /locus_tag="IWO52_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0356068.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            16270..18030
                     /gene="efrB"
                     /locus_tag="IWO52_001803"
     CDS             16270..18030
                     /gene="efrB"
                     /locus_tag="IWO52_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0356069.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            18125..18322
                     /locus_tag="IWO52_001804"
     CDS             18125..18322
                     /locus_tag="IWO52_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356070.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            18475..18960
                     /locus_tag="IWO52_001805"
     CDS             18475..18960
                     /locus_tag="IWO52_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0356071.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(19064..19657)
                     /gene="clpP"
                     /locus_tag="IWO52_001806"
     CDS             complement(19064..19657)
                     /gene="clpP"
                     /locus_tag="IWO52_001806"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0356072.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            19837..20364
                     /locus_tag="IWO52_001807"
     CDS             19837..20364
                     /locus_tag="IWO52_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0356073.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            20455..21192
                     /locus_tag="IWO52_001808"
     CDS             20455..21192
                     /locus_tag="IWO52_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0356074.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            21196..22113
                     /gene="miaA"
                     /locus_tag="IWO52_001809"
     CDS             21196..22113
                     /gene="miaA"
                     /locus_tag="IWO52_001809"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0356075.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            22115..23344
                     /gene="hflX"
                     /locus_tag="IWO52_001810"
     CDS             22115..23344
                     /gene="hflX"
                     /locus_tag="IWO52_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0356076.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         7601 a         4005 c         5051 g         6703 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt
     4861 gtctcacacc cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac
     4921 taaatatgtg aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc
     4981 aaaagcaact gcgaaaagat tacctttata tttacgctac ttgaagatgc tggatgactc
     5041 aggtataagt agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac
     5101 gatccgcaga gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt
     5161 cccttttctt atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc
     5221 attagtagga tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga
     5281 aaatctaaat atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga
     5341 tggagaaatg atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac
     5401 agtagcaatc tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa
     5461 aggaaatgta accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt
     5521 caatgttcaa tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa
     5581 ttactcagaa gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg
     5641 tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt
     5701 tgactaaaaa aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg
     5761 cacattcatt cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag
     5821 gtagcttggg agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc
     5881 acttgagagg caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt
     5941 cctagaatac gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg
     6001 gaagaaaata acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag
     6061 cattggttag aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca
     6121 atcgtaatcc cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg
     6181 accttgcaag atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg
     6241 cctggtatgg accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag
     6301 caagggattt ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg
     6361 aaagaagagt atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat
     6421 tacagtaggg aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt
     6481 gttaccttat acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct
     6541 caagcacgaa cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt
     6601 tatcatatga gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt
     6661 ccagaaacga tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa
     6721 gcgctcatcg gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca
     6781 gatgaatatg tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat
     6841 ccaaacgact ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt tatggatgat
     6901 gatggtacga tgaatgaact agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt
     6961 aaagcaatcg tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc
     7021 cacagtgtgg gtcattccga gcggacaggt gtcgttgttg aacctcggct gtctactcaa
     7081 tggtttgtaa aaatggcgcc gttagcgaaa aaagccattg ataaccaaga aacagatcaa
     7141 gcagtggaat tcttccctcc aagattcaat caaacgtttt tacgttggat ggaaaatgtc
     7201 catgattggg taatctcacg acaattatgg tggggacatc aaattcctgc atggtatcac
     7261 aaagaaacag gtgaaatgta tgtagggatg gaagcaccag cggatagtga aaactgggta
     7321 caagatcctg atgttttaga cacttggttt agttcagctt tgtggccttt ctcaacaatg
     7381 ggctggccag atacggaaag cgaagattat aagcgatact tccctactag cactttagta
     7441 accggttatg acatcatttt cttctgggta agcagaatga ttttccaaag cttggaattt
     7501 actggcgaac gtccatttga gaatgtcttg attcatggat tgattcgtga cgaacaaggg
     7561 cggaagatga gtaaatcatt aggtaatgga atcgatccga tggaagtaat cgaaaagtat
     7621 ggagcagatg ctctccgctg gttcttatcg aatggttctg caccaggaca agatgtccgc
     7681 ttcagctatg aaaaaatgga tgcttcttgg aactttatca acaaaatctg gaatgcttca
     7741 cgtttcgtta tcatgaatat cgaaggaatg accgtagaag agatcgattt atctggagaa
     7801 aaatcagtgg ctgaccggtg gatccttact cgtttgaatg aaacgatcga acgagtaact
     7861 gagctatttt atcgttttga atttggtgaa gcaggtcgac aactgtataa ctttatctgg
     7921 gatgattttt gtgactggta catcgaaatg agtaaagaag tactttatgg agatgatgct
     7981 gtttcaaaac aaaccacccg tagcatctta gtccatacac ttgatcagat cctacgttta
     8041 ttgcatccaa tcatgccatt cgttacagaa gaaatatggg aacatattcc tcatcaggga
     8101 aattctctag tagtggcaga atatcctgtt gtacgtccag agtttgatga tgaaacagct
     8161 tctaaaggga tggaagtatt gaaagaattg attcgagccg ttcgaaatat tcgttcagaa
     8221 gtgaatacac cattgtctaa accaattacg ttgctaatta aaacaaacga tgctaaaatc
     8281 gatcaatttt tagttgaaaa tacaagctat atcgaacgct tctgtaatcc cgaagagttg
     8341 acgatatcaa gtgagatcac tgcgccagat ctagcgatgt cagctgttct gacaggtgca
     8401 gaaatcttct tgccgttagc aggattgatc aatattgaag aagaaatcaa acgattagaa
     8461 aaagaactgg aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa
     8521 cgttttgttt ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat
     8581 tatttagaga agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa
     8641 gaatagaaaa agagagtgtg aaatcagcta gctattacta aaagctagct gattgaacat
     8701 cgttacagga aagagtttga gcctcactct tagcttcgat tcataaggag atgtgacata
     8761 aattttgtcg catctctttt ttagtctact cttattatgt aggagaagga gggaggtgga
     8821 tcatgtatca agagtcaata aagtagttga taaaatcgtt cctctctctg tttcttttct
     8881 ctttgtcata cttttatttt tcttatgtgt tctatttgtt ataataaaag agaaatgggg
     8941 gaattgttga tgacgattga agaagcaata gaatggatcc atagtcgatt accatttggt
     9001 tcacgtcctg gccttgaccg agtcgaagct ttattagaag tggtagggca tccagagaga
     9061 aaagtaaaaa cgatccatat cgctggaaca aacggaaaag gttctactgt agcttatttg
     9121 agaagcctat tagaagaggc tgggctgaca gtaggtacat ttacttcacc ttatatcgaa
     9181 acgttcaatg aacggatcgc aatcaatggc aaaagcattc cagacaatga tttgatccag
     9241 cttgtaaaaa aatatcagcc actgatcaaa caacttgatc aatttgaagc agtagctggt
     9301 attactgaat tcgagacatt aacagcaatg gcatttgatt attttttgat gaagaaagtg
     9361 gacgtagcga ttatcgaagt tggcttaggc ggcttattgg attctaccaa tgtagctgta
     9421 ccaatgctga cgggaataac aacgattgga cttgatcata ccgaaatact tggagataca
     9481 attgaagaaa tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc agtagtaaca
     9541 ggaaatattg ccccagaagc ttttactgtc atccaacaaa cagctaaagc aaaaaatgca
     9601 ccgctttatg tatacgggga aaattataaa ataaactatc tgcatcctga tgagatgtgg
     9661 ggagaagtat ttgaattttt taatgaaata ggaaagatcc ctcaattaac cactccgctt
     9721 ttagggcaac atcaagctga aaatgcagga gtagccatcg aattgtattt tgtttactgt
     9781 caattggaaa agctgccttt ccgagaaaaa gatgttttga agggattgaa gaaatctcga
     9841 tggccaggaa gaatggagcg tttgagtaac gagccattag ttgttttaga tggtgcccat
     9901 aatacgcatg caatgaaccg gcttgttgag aatttgaaaa aagaattcaa aggatatcaa
     9961 atcaacattt tgttttccgc cttacgaact aaaaatgtta gagaaatgct agaacgattg
    10021 cagcaagtac caaatgtcca tatttatttg acgacctttg aacatccaaa agccatcgaa
    10081 ttaagtgaag tatcagattt agcaagtgag agagtatcaa tcgtttcatt gtggcaattc
    10141 ggattagcag atatattgga aaagatgaca aatgaagacg tattagttgt gaccggttca
    10201 ctttatttca tttcagaagt tcgccattta ttaatacaat taggaggaga tcatgaagta
    10261 taatgcagtg atttttgaca tggatggttt gctgttcgac acagagttag tgtattacga
    10321 agccacacaa gtagtagcag acaagatggg ttttccttat gataaagacc tgtatttaaa
    10381 attcttaggt gtttcagatg aagaagtatg ggcgaattat catatgattt tctcggaatt
    10441 tggcaaaaca agtgtacaaa gattcataga tgattcatac caggaaacgc ttgaacgttt
    10501 ttcgcaagga gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga
    10561 acatcagatt cccaaagttg tcgcttccag taaccaacga gcagtcattg aacttttatt
    10621 gaagaagaac aatctgtttg aacgatttga gacgattgtt tctgcagaaa atgttcaacg
    10681 agcaaaacca gacccagaaa ttttcttgtt agctaatcag aagctaggta cagcgaagaa
    10741 agaaacattg attttagaag attcacaaaa tggtatttta gcagcttctg cggctgagat
    10801 cccggtagtc atggtaccag atctgcttcc accatcccaa gaattgatca aaaaaacagc
    10861 tggggttgtt tcttctttac atgaaatccc tgcattgtta aaataaaaat ccatctcttt
    10921 tcgtatctta ttttagataa gatagaaagg agatgttttt ttgggaaaac gattgatcaa
    10981 agaagtacct actagctctt taccaaggga acgtatggaa atttatggcg cggaagcttt
    11041 atcggatcag gagcttttgg caatcttact gcgtaccggt caacaccctt atagtgtgat
    11101 gtcgattgct ggaaatctgt taaaaacgtt tggaggactg gcctctttac gacaggctac
    11161 tttgcatgag ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc
    11221 cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa agagcggctc caccggttcg
    11281 taccagctac gaattggccc agcaattgat tctggaaatg aaagatcaca aacaagaaca
    11341 tttggtttgt atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat
    11401 cggttcgctg aatcagtcag tcgctcatcc tagagaaatt ttccattatg ctgtacgtta
    11461 ttgtgcagca aggatcgttt tagcgcataa tcatccttct ggtaatgtta ttccatcgca
    11521 gcaggatatg aattttacca aaaggataca aaagtgtgga gaaatgatgg ggatcactgt
    11581 actggaccac ttgatcatcg gacgcaaacg atattttagt ttgagagaag aaggaatgat
    11641 ggaagagaag taagttgctt tcttgcaaaa aaaatgaagc aagtgtacaa ttatattgta
    11701 attttgtttg ggaatagaag ttgaaacatc tgttgcacct ctatcgtaca cgagcaatgt
    11761 tacaatttaa attagtgctt ttggcacgag gaggatatta ttatggaaaa cggaacagta
    11821 aaatggttca acgctgaaaa aggatttgga tttatctcac gtgaagatgg aagcgacgta
    11881 ttcgttcact tctcagctat ccaaggtgac ggattcaaaa cattagaaga aggtcaagca
    11941 gtgactttcg acgttgaaga atcagaccgc ggcccacaag ctgcaaacgt tgtaaaagct
    12001 taattttatc tgtaaatcaa attgatggta gacatcaatt tttacgagag aacaaagcga
    12061 gtaacaaaaa tacctgccac aagagacaat ttctcttgtg gcaggtattt ttttgtatga
    12121 aaaagtaaat aaatttaaga ggctatgaaa gagccacctt gtatcaagga ataagaacaa
    12181 acaaaaatgg cttctcatgt ttttcatttg ttttctctta tattcaggac aaggtgctga
    12241 tttcataacc tcttatttat tcttcatctg gtgtcattac cattggcaaa atcattggat
    12301 gtctttctgt acgttcgaat aagaaaggct gaagcgcgcc agtcatcgct tctttcaatt
    12361 tatattctga acagtcaggt ccattcaaac attctcttaa agcgtggaat aagattcgct
    12421 gagcttcatt gatcatttct cctgattctc gcatgtagac aaatccacga gagagaatat
    12481 ctggaccagc caagatttct tttttcttga tatccactgt cgcgacagct aatacaagac
    12541 cttcttctga aagcaggtga cggtcacgta aaacgacatt accaatatca ccgatcccgc
    12601 taccatcgat atacacatct ccagccacaa aatgcccagc aggacgcgca gaatcgctag
    12661 ttaacgctaa aacatcgcca ttttccatga tgaagcagtt ttctgcagga acacctacat
    12721 cttgtgctag agaagcatgt actttcaaca tgcggaattc tccgtggaca ggaataaaat
    12781 attttggctt catcaaacga agcattaatt tttgttcttc ttgtccaccg tgaccggaag
    12841 tatggatgtt attgattttt ccgtgaatca cttctgcacc agcttcagac agcaaattga
    12901 tcaaatgatt gacacttgtt gtattaccag gaataggtga acttgaaaag atcactgtat
    12961 cgtctggctg gatttggatt tgacggtgag taccattcgc gatgcggctc aacgcggcca
    13021 tcgcttctcc ttgagaacct gtacatagga tcatcgtttc attggcaggc aagcgattga
    13081 tttcgtgtgc atctacaaat gtgccgtctg gaactttgat atatcctaaa cgttgaccgt
    13141 tgacgatggc attttccatg cttcgtccga aaacagcgat tttacgacca gttgccacag
    13201 cggcatctgc tgcttgctgc aaacggaaga tgttagaagc aaagctggca aaaatgatac
    13261 gtccttcgat ttttgaaaag atcttcaaaa tagaatgccc aatcgttttt tctgatttag
    13321 taaatgttgg aatctcagca tttgtactat cagataaaag gcacaagact ccttcttccc
    13381 cgatacgagc cattttatgc aagtttgctg gttcgccaac aggagtgaaa tcgaatttga
    13441 agtctccagt cgctacgata tttccagaag gtgttttcac cacgatccct aatgcatctg
    13501 ggatactatg ggtcgttctg aaaaagctga cagaagtctt tctaaaacgg atgatcgtat
    13561 cttcattgat ctcatggagc tctgcatctc gaagcaagcc atgctcatct aatttgtttg
    13621 tgatcaacgc taatgcgaga ggaccagcat agataggaat atttgcttgt ttcagcaagt
    13681 agggtattcc gccgatgtgg tcttcgtgcc cgtgagtaat caccaaggct tttactttgt
    13741 gtaagttttg tatgatgtag ctgtagtcgg gaatgacata gtcaattcca agtaaatcgt
    13801 cttccgggaa tttgatcccg gcatcaatca aaatgatttc atcttggaat tgtaccccat
    13861 acgtattttt cccgatttca cctaatccac caatagcaaa gacgcctgtc tcgttatttt
    13921 tgatggaaag tttcatatta aaactccact aatgagaaat cagcattttc tttttcgtac
    13981 tctagatgac ttccttctag aagttgaacg aattcaatat tgtatggggt attttcttcc
    14041 acacgttggc gagcgtctac atcgcttttt gcctcaatgt aaagagattt tgtttcttcc
    14101 cgtttagggt tgcgtacttt tgtttcttga taataaactt tatagatcat tgttttgtct
    14161 cctttatctg cgtaaagcag tgatttagct agttgatttt tttagacaac acaaaacgag
    14221 gggacaagcc acctggtttt tcatatgctt tgactagaat actggattcg agtcaaagag
    14281 acaagcctcg tttttgctta tctgttcgga tattttttca accgtacctt ctgtataaaa
    14341 aatatacaac aattaattgt tattctagca taaaacagca aataagtata gaaatcaact
    14401 agattgaaag aaaaaatcaa tgtttatcat cccgctataa tcataatgac ttttttatga
    14461 gagaagtgtt ctattaatag gctcgttgtg ctatcatttt tagtaatgaa aatgtaacaa
    14521 aagattaaaa ggagatgaga aagtgaaact aatgtggcat tataccatgc gttataagaa
    14581 attcttgttt ttcaatttca tatgcgtatt tggttttatt ttgattgaat taggacttcc
    14641 tacgatttta gcacgtatga tcgatgtagg aatccgcaat gatgattttg attatgtgaa
    14701 gcaacaaggg atatttatga tcgttattac tgtaatcggt attattttga atatcttgtt
    14761 aggttatttt ggttcgagga ttacgactaa tatcgtagca gatattcgag atgatttgtt
    14821 cgaaaagatc caaagttatt cgcatcagga atacgaaaat ctaggcgtat cttcattgat
    14881 cactcgtaca acaaacgatg cttatcagat catgttgttc ctgcaaaata ttttacgtat
    14941 cggttttatg gcaccaatga tgttcattgt cagtttgtac atggtcatgc gtacaagtgt
    15001 gacactcagt ttgtatgtag taggtgcatt acctttacta ttattggcag tcgtagcgat
    15061 tgctaaggtg tctgaaccgt tatctaaaaa gcaacaaaaa aacttagata aaatcaacag
    15121 tatcttgcgg gaaaatcttt caggattgcg tgtgattcgt gcattcgtca atgaaaagtt
    15181 tgaagaaaaa cgttttgctg tagtcaatga gaattatgct tctagttcaa aaagcttatt
    15241 ccgtttgatg gctgttgcac aaccgggttt ttatttctta tttaatatcg taatggtatt
    15301 gatcatctgg agcggggcag tgcaaatcga tgcaggcgag cttcgtgtag gagatttgat
    15361 tgcgtttatc gagtatatat tccatgcctt gttctcattc atgttatttg ctagtgtgtt
    15421 catgatgtat ccaagagcag ctgtttcagc tggacgtatc caagaagccc tagatgccaa
    15481 accttcgatt gtagaagatc caaatggtat atctgataca aaaactaaag ggtatatcga
    15541 atttcaaaat gtaacatttg cttatccagg ccattcgcaa gagccagtga tacgaaatgt
    15601 cagttttaca gcctctccag gagagaccgt tgcttttatt ggaagtaccg gtagcggaaa
    15661 atctacgttg atccaattga tccctcgctt ttatgatgtg agtgaaggaa agatcttgat
    15721 cgatggagta gacgtgagag attatcggct aagcaagttg cgagataaga ttgggtttat
    15781 tccgcaaaaa gcgctgctat ttactggaac gattgcagat aatttgcgat acgggaaaga
    15841 agatgctacg caagaagaaa tggaacgtgc agctgagatt gctcaagctt cagaattcat
    15901 ttctcgaaaa ccagatggat ttaatgaaca tttatcagaa ggtggttcca atttttcagg
    15961 tggacaaaaa caacgattgg caatagccag agccatcata cgccgtccgg aaatctatat
    16021 ttttgatgac agcttttctg cattagatta tcaaacggat gcgaagttac gtgctcgtct
    16081 gaaaaaagaa acgacagaat ctactgtatt gatcgtagca caacgtgtgg gaacgatcat
    16141 gcatgcagat aagatcatcg tattaaatga aggagaagtc gtcggtatgg ggacgcatcg
    16201 ggagttgttg gaaaaatgcc cgatttatta tgatattgcc gcttctcaat tatcaaagga
    16261 ggaattagca tgaaaaaaac cttttcttcc ttaaaacgtt tagctcgata tatcagaccg
    16321 tatcgtgaga cttttctttt agtgatccta tttacagttc tgacagtggc tttcaacgtg
    16381 gctttgccat atgttacagg acttcctact acagaaatca gccgtaatat tgccaatggc
    16441 gaatcaatca atttttctta tatattcaaa tgtctcattt ggatcacagt tgttggaata
    16501 gggtattgta tttcgcaatt actttctggt gttttgatga caaatgtcgt tcaaagtgcc
    16561 atgcatgatt tgagaagaga catagaagaa aaaatcaatc gtcttccggt ttcatatttc
    16621 gataaaaacc agcaaggaaa tattttgtct cgtgtgacga atgatgtaga tgcagtcagc
    16681 ggtgctttac aacaagcttt tattggtatc gtaaatgcga tattgggaat tacgttggct
    16741 gcagcaatga tgttttatat ccagccgatg atggcgctga tctctatgat catgattcca
    16801 acatcgatct ggatctcaaa acgagtgatc aatgcttcgc agaaatattt tcaaagcatg
    16861 cagaattctc ttggtgagct aaacggctac gtccaagaaa atatgacagg ttttagtgta
    16921 ttgaaagtat atggacgcga aaaagaaaca ttggaaggat tcaaaaaagt taatcacagc
    16981 ttgaaatact ttgggtttcg tgcagcattc atttctggct tgatgatgcc tctagttcaa
    17041 ttaactgcct atgctactta tattggtatg gctgttctag gaagctttta tgttattact
    17101 ggggcgatcg ttgtcggtca actacaagca ttcatccaat atatttggca aatcagtcag
    17161 ccaatgggga acgtgacaca attgtcttca gttttgcaaa gtgcatcagc ggcaacgaaa
    17221 cgtgtattcg agattttaga tgagccagaa gaaaaagaaa acaaagtaga tgcttcactg
    17281 ccagaaacga ttttaggaga tgtgacattc gatcatgtcg attttgcata tgatccgaag
    17341 aaaccattaa tccaagattt gaattttgaa gtgaaagctg gtcaaaccgt agctgtggtc
    17401 ggtccgaccg gtgccggcaa gacgacattg atcaatctat tgatgcgttt ttatgacgta
    17461 gacaaaggag caataaggat tgatggaatc gacaccaaat caatgagtcg cagtgatgtg
    17521 cgttcgctat ttggtatggt cttgcaagat gcatggcttt atcaaggaac gattgcagat
    17581 aatatccgct tcggaaagct tgatgcgaca gattatgaag tagttgatgc tgcgaaaaca
    17641 gcaaacgtag atcattttat ccgaacgatg ccagatggat atgatatggc catcaattct
    17701 gagggagaca atgtgtcttt aggacaaaaa cagttgctga cgattgcccg agcagtggtc
    17761 tccgatccta aaatcttgat tttagatgaa gcaacaagtt cagtagacac ccgtttagaa
    17821 gcgttgattc aaaaagcaat ggatcgcgtg atggagggac ggacaagttt cgtcatcgct
    17881 caccgtttat cgacaatccg tgatgcagat ctgattctag tgatggatca aggtcagatc
    17941 attgaaaaag gaacgcatga gagtttattg gcacaaggtg gattttatga aaaactgtat
    18001 aacagccaat ttgctgagga agcagaataa gtcatataag aataaaacag tttaaaactt
    18061 attttttgtt ctctattctt gataatggta aagtaaagtt atcaagaaaa gtgaggacac
    18121 ggagatggaa cagtttttaa atgaaaaaat tactgaagcc aaaagagaag ttgatcgttt
    18181 aacgcacgtc tgtcagacga agccttttga ttcagctgat ttttttgaaa atgagtttca
    18241 aatcgaacgc ttggcagcac aaatagaagt gtacgaatta attttaaaaa aagtaataga
    18301 agaaaatata gatgtacaat aaaaacaggt aaaacgtcag ttaccagata ctgtaaatag
    18361 acatctggcc tgagcaggtt ttacctgttt tttttcttat gaaataaata tatgctatga
    18421 tacctttatt caataggatt agtttctaca aaaaatgaaa ggagaaaaat aaatatggaa
    18481 cggtttatag catcaaatgc cacagtgatt ggtgatgtga cattgtcaga agatgtgacg
    18541 atctggtatc aagctgtttt acgcggggac agtaattgga taaaaatagg acaaagaacg
    18601 aatatccaag atggtacaat catccatgtc gatcatgatg cacctgtaga tattgcggag
    18661 aatgtgacgg taggacatca gtgcatgcta catggctgta caatcgaaaa aggggcgctg
    18721 atcgggatgg ggaccacgat attgaatcat gcggttattg gtgagaatag tttgattggt
    18781 gctggatcat tagtaacaga aggaaaagtt attcctccta atgtcttggc ttttggtcgc
    18841 ccagctaaag tgatccgtcc tttgacaaaa gaagaaatac aaaaaaacaa agaaaacatc
    18901 cagcattatg tcgagatagg tcggatgcat gcgcaaggaa aattcaagga gcaaagatag
    18961 gcagtagcat ccataaaata aaaaaggatg gtcagtttga gttgaccatc cttttttatt
    19021 tggtttagat aagagagctt aaaaaagctt cctttgaaac agattaattt aatgaagaac
    19081 tgttttccat gacttcatcg atcaatccat atgcttttgc ttgttcagct gtcatatagt
    19141 tgtcacgatc tgtatctttt tcaatcactt caagcggttg tccagtgcgt tcagctaaaa
    19201 ttttgtttaa gcgttcgcgt gtttgcaaaa tatggcgtgc cgcaatttcg atttcagttg
    19261 cttgcccttg agcgccgcca agaggttgat ggatcatgat ctcagcattt ggtaaagcaa
    19321 atcgtttacc ttttgtacca gctgtcagca agaagcttcc cattgaagca gccataccca
    19381 taacaattgt ttggacatct gctttgacaa agttcattgt gtcgtaaata gccatacctg
    19441 ctgtaactga accaccagga gaattgatat aaagatagat atctttttca gaatcttgag
    19501 cgtctaagaa aagcaattgg gcgatgatcg aattagcaag atcatctgtt acttggccgc
    19561 ttaacatgat gatacggtct ttcaataaac gtgagtagat atcgtaagct ctttcacctc
    19621 gtgaagattg ttcaatgact gtaggaatta aattcataat agagttcctc cttgatttcg
    19681 tttagaaata gtgtaccata atttagtttc taatacctaa aatgtagtat acatttttgg
    19741 tcaaaaaagg tcaaataaaa aacttaggca aattagtcgc ataaacccgc tttatggtat
    19801 gataagcaga gaaacaaaac aaggaggaac aacagattga tagttggtat tactggcctt
    19861 atcggagtgc tgacaggtgc tgtagttgtc tatatcggca tgatactgca aatgcgtcac
    19921 ttggaaaata aagaacagcg tagacgtgaa ttggaattga aagtcaaaga aatcgagacg
    19981 ctgaatcaat taaataaaaa gatcaacgag atcttgcaaa aacggacaac gctgatggaa
    20041 gaatatgtca gctttgatgc atttgatgat tgttatatca cgattgacga ttttgcgtat
    20101 ctccaatcat tttcagcgca aaatagtttt tatctgccaa attatatttt agacgagcta
    20161 tttaagaata tctcccatcg aaaagtgatt ttatctccag atgaaacaat gaagattggt
    20221 ggatatgctt ataaaggcgg acgcgtgatt ttagagaatt ttacggatag tctgactgaa
    20281 atgatcaatg aaaaaaaagc tcagttgaag actatgacgc gccagccgat tggtttcttc
    20341 tcgaaagatg atgtttggag ctgatatttt gaagcattgc cgttgcacat gagagcgtgg
    20401 taatgctttc tttttttctt aatgatacaa tagaactgaa cggagtgaag aaagatgtta
    20461 caaatctatg cccatcgcgg aagcagcggc acccacccag aaaatacgct tcctgcattt
    20521 gcagaagctg tacgagtagg tgcagatggt atcgaactag atgtccatct aagcaaagat
    20581 ggttatttga tcgttatgca cgacgaagaa gtggaccgga cgactaacgg taaagggctg
    20641 attcgagaaa aaacattgga agaactcaaa aaattgaatg cagggggcaa attcagcaaa
    20701 gaatttccag cagctaaagt tcctgaattg agagaagtgt taggactgct cctccaaaaa
    20761 aattaccgag ggatgctaat gattgaaatt aaaactgatc agtatgaata tcccggtatc
    20821 gaagaaaaat tagccgaaac attaaaaagt agagattggc cttttcaaca tgcatattgc
    20881 agctttcatt tggattcttt ggaaaaaatc gcattactgg aaccggagat acagctggac
    20941 ttattgatga gtacttctga taaaaagatc caactagcag cagaaacccc ttatatagaa
    21001 ggagtacatc caaaaaatac ttggttgttc gaacatccag tccaggcagc tgattttcca
    21061 aaagcaattc gtgcatggac gattaacgat gaagaagaaa tggccaaatg tcaagaactc
    21121 aaaatagatg gaataataac tgattttccg gagaaagctc ttcggtggaa acaatcaacg
    21181 aaaagtaggt aaaatatgga aaaagtatta gtgatcgtcg gtccgacagc agtcggtaaa
    21241 acagcgctaa gtgtggaact cgcacaacta tttggaggag agatcatcag tggtgattca
    21301 ttgcaaatct ataaaaatct ggatatcggt acagcaaaaa tcaaagaaga agagatgaat
    21361 aatgttccgc atcatttgat tgatgtgatc gaacctactc aacgttactc agccgctgat
    21421 tttcagcatt caggtcgaca attgattacc gagattgcga accgagggca cctacctatc
    21481 atagctggtg gtacgggtct ctatatccaa tcccttctat atgattatca gttaggagcg
    21541 acagaagaag cgaatcctga gattagaaaa aagtatgaaa cgttagctga aacaattggt
    21601 tcccaagcat tatggaatca gttgaaagaa aaagacccgg cagctgctga gaaaatccac
    21661 tggaacaatc aacgcaaagt gataagagct ctggaagttt tagagacgac aggacatagt
    21721 atcacggcac ctaaagaaga gccaaagcaa ttgtatgatt acctcatgat tggattagat
    21781 acgaagcgta gtattcttta tgaacgaatc aatcaaagag tcgatttgat gctagcagaa
    21841 ggtttagtag cagaagcgcg taaggtgtat gagcttggag aagtccaggc cagtcaaggt
    21901 attggatata aagaattttt cccttatttt gaagggaaag aaacattgga agaagcaaca
    21961 gaacaagtca aactacattc gagaagatac gcgaaacgtc agttgacgtg gtttcgtaac
    22021 cgtttggatg cacagtggtt tgatcttata agtgagccaa atcaaaaaag tcagattgag
    22081 caaacaatca aaaaatggct ggaggcggaa taaaatggaa gaaaaagtga tcatcgtagg
    22141 ggtagaaacc gaagcaaacc agcgttactt ttcagagtca atggaagagc tagttcagct
    22201 gacgaatacc gcatcaggag aagtcgtatt cacaattaca caaaagagac cacaagtgga
    22261 tcggcaaacg attatcggta aagggaaatt acaagaatta gttcaacaag cggatgcgca
    22321 tgaagcggat ttgatcatct ttaatcatga gctaactccg agacagagcc aactgattac
    22381 cgatgctgtt ggtcttccgg taattgatcg cgtgcagctt atcttagata tttttgcgat
    22441 gcgtgctcgt tcgaaagaag ggaagctgca ggttgaactt gctcagttag aatacctgct
    22501 gcctcgttta gtagggcaag ggaaaacatt atctcgtctt ggcggtggga tcggaacaag
    22561 agggccaggt gaaacaaagt tagaaacaga ccgccgacat atacggaata aaatttttgc
    22621 tgttaagaaa gaattgaaag aagtagaagc tcatcgagag cgcaatcgcc aaaaaagaaa
    22681 aaatagcgag atttttcaga ttggacttat tggctacacg aatgccggga agtcaactat
    22741 cttgaatcta ttgacacaag cggatactta ttcgaaagat caattgtttg caacgttaga
    22801 tccattgact aagagatggc gttttgctga aggatttgag attactgtaa cggataccgt
    22861 cgggttcatc caagacctgc ctacgcaact gatcgatgct ttccattcta cattagaaga
    22921 aagtcaaaat atggatcttt tgctacatgt agtcgatgct agttctccag atcgtatttt
    22981 acaggagcag actgtcttaa aattgatgga tgaactgaat atgaaagaaa tgccgatact
    23041 gactgtttac aataaggcag accagatcga cccagcgatg ttcacgccta cgctattccc
    23101 aaatgtattg atttctactc agacgaaaga aggtaagatg gcgttgatcg aagcaatcaa
    23161 acgtcagctg atggaactaa tgacccctta tacactttct gtgccaagca acgaaggaca
    23221 gaaattaagc gagttgagaa gacaaacaat ggttttggac gaagcgtaca tggaagaaac
    23281 aaacgaatat gaagttaaag ggtttgcaaa aaccaattca aaatggttaa gaaaaaacca
    23341 ataaaatttt atttttttaa
//
LOCUS       DACEDO010000045        22986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEDO010000045 DACEDO010000000
VERSION     DACEDO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.045"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(110..565)
                     /locus_tag="IWO52_001811"
     CDS             complement(110..565)
                     /locus_tag="IWO52_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356077.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="IWO52_001812"
     CDS             complement(719..1927)
                     /locus_tag="IWO52_001812"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0356078.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="IWO52_001813"
     CDS             complement(2039..2371)
                     /locus_tag="IWO52_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0356079.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="IWO52_001814"
     CDS             2519..3394
                     /locus_tag="IWO52_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0356080.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="IWO52_001815"
     CDS             complement(3476..4057)
                     /locus_tag="IWO52_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0356081.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="IWO52_001816"
     CDS             complement(4063..5322)
                     /locus_tag="IWO52_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0356082.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="IWO52_001817"
     CDS             complement(5324..6370)
                     /locus_tag="IWO52_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356083.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="IWO52_001818"
     CDS             complement(6605..6880)
                     /locus_tag="IWO52_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0356084.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /locus_tag="IWO52_001819"
     CDS             complement(7123..8433)
                     /locus_tag="IWO52_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0356085.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="IWO52_001820"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="IWO52_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0356086.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="IWO52_001821"
     CDS             complement(9979..10659)
                     /locus_tag="IWO52_001821"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0356087.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="IWO52_001822"
     CDS             complement(10708..11343)
                     /locus_tag="IWO52_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0356088.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="IWO52_001823"
     CDS             complement(11409..12812)
                     /locus_tag="IWO52_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0356089.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="IWO52_001824"
     CDS             complement(12799..13830)
                     /locus_tag="IWO52_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356090.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="IWO52_001825"
     CDS             13861..14082
                     /locus_tag="IWO52_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0356091.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="IWO52_001826"
     CDS             complement(14247..14984)
                     /locus_tag="IWO52_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0356092.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="IWO52_001827"
     CDS             complement(15137..16477)
                     /locus_tag="IWO52_001827"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0356093.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="IWO52_001828"
     CDS             complement(16714..18237)
                     /locus_tag="IWO52_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0356094.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="IWO52_001829"
     CDS             complement(18615..19226)
                     /locus_tag="IWO52_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0356095.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="IWO52_001830"
     CDS             complement(19556..20272)
                     /locus_tag="IWO52_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0356096.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="IWO52_001831"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="IWO52_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0356097.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21628)
                     /locus_tag="IWO52_001832"
     CDS             complement(20831..21628)
                     /locus_tag="IWO52_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0356098.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="IWO52_001833"
     CDS             complement(21598..21969)
                     /locus_tag="IWO52_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0356099.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="IWO52_001834"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="IWO52_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0356100.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6446 a         4912 c         3900 g         7728 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc gttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca attaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcaggt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacactt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggttt
//
LOCUS       DACEDO010000046        22244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEDO010000046 DACEDO010000000
VERSION     DACEDO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.046"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..400
                     /locus_tag="IWO52_001835"
     CDS             2..400
                     /locus_tag="IWO52_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0356101.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IWO52_001836"
     CDS             384..1079
                     /locus_tag="IWO52_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0356102.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1673..2560)
                     /locus_tag="IWO52_001837"
     CDS             complement(1673..2560)
                     /locus_tag="IWO52_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0356103.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2745..3584
                     /locus_tag="IWO52_001838"
     CDS             2745..3584
                     /locus_tag="IWO52_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0356104.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3780..4700
                     /gene="sgrA"
                     /locus_tag="IWO52_001839"
     CDS             3780..4700
                     /gene="sgrA"
                     /locus_tag="IWO52_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0356105.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            4998..5354
                     /locus_tag="IWO52_001840"
     CDS             4998..5354
                     /locus_tag="IWO52_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356106.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5357..6514
                     /locus_tag="IWO52_001841"
     CDS             5357..6514
                     /locus_tag="IWO52_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356107.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6540..6980)
                     /locus_tag="IWO52_001842"
     CDS             complement(6540..6980)
                     /locus_tag="IWO52_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0356108.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7140..7646
                     /locus_tag="IWO52_001843"
     CDS             7140..7646
                     /locus_tag="IWO52_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0356109.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7814..9727
                     /locus_tag="IWO52_001844"
     CDS             7814..9727
                     /locus_tag="IWO52_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0356110.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9732..10733
                     /locus_tag="IWO52_001845"
     CDS             9732..10733
                     /locus_tag="IWO52_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0356111.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHFDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10935..11141)
                     /locus_tag="IWO52_001846"
     CDS             complement(10935..11141)
                     /locus_tag="IWO52_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0356112.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11787..12635)
                     /locus_tag="IWO52_001847"
     CDS             complement(11787..12635)
                     /locus_tag="IWO52_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0356113.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12799..13701
                     /gene="gnd"
                     /locus_tag="IWO52_001848"
     CDS             12799..13701
                     /gene="gnd"
                     /locus_tag="IWO52_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0356114.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13703..14704
                     /locus_tag="IWO52_001849"
     CDS             13703..14704
                     /locus_tag="IWO52_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0356115.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14701..14985
                     /locus_tag="IWO52_001850"
     CDS             14701..14985
                     /locus_tag="IWO52_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0356116.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15018..15815
                     /locus_tag="IWO52_001851"
     CDS             15018..15815
                     /locus_tag="IWO52_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0356117.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15829..16662
                     /locus_tag="IWO52_001852"
     CDS             15829..16662
                     /locus_tag="IWO52_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0356118.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16680..17537
                     /locus_tag="IWO52_001853"
     CDS             16680..17537
                     /locus_tag="IWO52_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0356119.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17559..18068
                     /locus_tag="IWO52_001854"
     CDS             17559..18068
                     /locus_tag="IWO52_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0356120.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18105..18506
                     /locus_tag="IWO52_001855"
     CDS             18105..18506
                     /locus_tag="IWO52_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356121.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18618..18971
                     /locus_tag="IWO52_001856"
     CDS             18618..18971
                     /locus_tag="IWO52_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356122.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19005..19448
                     /locus_tag="IWO52_001857"
     CDS             19005..19448
                     /locus_tag="IWO52_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0356123.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19465..20550
                     /gene="uxuA"
                     /locus_tag="IWO52_001858"
     CDS             19465..20550
                     /gene="uxuA"
                     /locus_tag="IWO52_001858"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0356124.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20543..21256
                     /locus_tag="IWO52_001859"
     CDS             20543..21256
                     /locus_tag="IWO52_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0356125.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22008..22208
                     /locus_tag="IWO52_001860"
     CDS             22008..22208
                     /locus_tag="IWO52_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0356126.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7659 a         3419 c         4600 g         6566 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tccaaaaatc acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga
     1681 atatattttt tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga
     1741 ttgttttatc catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct
     1801 cagatttgta tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat
     1861 aatagctttg ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt
     1921 tttttagttt aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa
     1981 aattcatttt tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct
     2041 tggagaaatt ttctccttca tttgcttctt tttttgcttt atctgctcct tctttagtcg
     2101 aaaatagaat gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat
     2161 ctttatctgt taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa
     2221 agagtttctt atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg
     2281 gaccgagttt tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt
     2341 ttccgtatac atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat
     2401 ttgatttaac ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg
     2461 ttgcaatcgt ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa
     2521 ggcacataat tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag
     2581 tctaacagaa gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt
     2641 agttcgtaaa aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa
     2701 gaaagaaaag ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc
     2761 ggttgattgg atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata
     2821 atgcacttgc ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg
     2881 tcggacattt agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa
     2941 gaatagaaga ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc
     3001 tcgttgttgg aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt
     3061 atcggttaga ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta
     3121 ttgcagtggg ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta
     3181 gatttggtcc gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat
     3241 tgaatcctaa catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca
     3301 gcgcacttat ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga
     3361 atcggacaca aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt
     3421 tctccactct gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag
     3481 catctatatc aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat
     3541 ataaaacaaa gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa
     3601 ggctgatatc ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat
     3661 tcaagcgaat acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca
     3721 ttttcatttt atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa
     3781 tgaagaaaac agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga
     3841 ttggaatcag tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa
     3901 ctgatcggat tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca
     3961 aaagcgtgaa tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa
     4021 gttcagaaaa agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg
     4081 agtatacaat aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag
     4141 ttgatttgat tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca
     4201 gttggacagt tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag
     4261 aaaacacgag cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca
     4321 cgagcagcga aagcagtacg gagagtacga gtagtgaaag tagcacagga agcatgagta
     4381 gtgagagtag cacaggaagc acgagcagcg aaagcagcac aggaaaagta aataatgaac
     4441 ttgatttggt acctatcata ccaactaagg aacaaaagag aaatgcaggg agcaacctaa
     4501 aaagtgtgct aaaacagcct gattttgttt ctcgtggaat tgatcatcaa gaaaaagcga
     4561 aagaaacaga taatagaaaa agcgatttac cagaaactgg tagtagaacc ttgaacaagc
     4621 taattacatg gatgggtgtg ttactcattt tgattgtagg agcaagctat tttcgttctc
     4681 tcttccatcg agtgaaataa agaataaaag caacagatct aaaattgaat aaaacgatag
     4741 atttttgagt gaatatttca ttcgagagtg ctgtattgcc tggcgaagtt gtagctttgc
     4801 cagatctatt tttgtttact aacatatttg agaaacagga aataacagga acacaaatgg
     4861 aaaataagag caagaataat tgacaatgca cactgcgcat ggtagtatct ctgaaaaatc
     4921 agtaacgaat ctatttgtac gttattcatg tagctgtcgg aataagtcta aataatagat
     4981 aaaaaggagt atgtactttg tttgaagaat ttattgatat caatgaaagg caagtatatc
     5041 aatttttgaa ctattgttat gaaagagacg agaaattata tgtggtgaag gatatcgctt
     5101 tagatctaaa ctacaccctg gctaagatga attcggtgat tcaacaagca gaatcgtttt
     5161 gcgaaaggta tccagaatat aaattatctt ttctttcaga aaacaagatg ataaaagtcg
     5221 agttttcttc tcagttttta ctgagtaaag tttattctat acttttagag ggaacgattg
     5281 gttatatttt actggattca ctttacaaag gtacttatca atccttagaa aatcttagtc
     5341 aaaaaattat ataagtttgc gtacgattca aagaaaatta agagaactta atactatttt
     5401 gaaaaactat catttatctt gcagcttgaa aaaaggtaat ccgatagaag gagaagaata
     5461 ccgtatccga tacttttttc acttgatgta ttggcagtca ttcgacgaaa atcattctga
     5521 tatattatca agtataaaaa ataaacgtca actattagaa aaatttgcga aacatgcgcc
     5581 atatacgcgt agtatagata gaaagaaatg gatcagccta ctaagaattt cgttgcaacg
     5641 aatgaaacaa ggccactcga ttacgtatgt cccagaagag atgaacaaat ttgtgcatcc
     5701 attgataaca aaagaagagt ttacgcatta catactagag cctttttttg aagcaaactt
     5761 ctggtcgatg aatcacatca ctccaacaga gcaagcttac ctttatttta tgtttggtgt
     5821 aatgaacacc tatttggaag aagatttgtt ggatactcca gataaagtac aagaaagttg
     5881 gggctggatt gaatcgtcta ttgtagaaaa ttttgaaaaa aacttcaaga tttgttttac
     5941 aacttcagaa aaaaattatc tatatgtcaa tttggcaatg atccatttgt attcaagggt
     6001 ttttgggaca aaaaagaagg tggatgcttt tggaaaatca gcagaagaca gagattttca
     6061 gcatatattt ccagtgatgt atccaatcat gcaacagttt tttagtgggg tagctcgcag
     6121 aattccagag cttaatactt attatgaaaa gaacagacgg ttgatttttc aatactgtat
     6181 gttagtcagg gatattttta taaaacacga acaacctgtc atattctata ttcaatcaaa
     6241 gtatggtaaa acacaggaat tatgggcaaa gaaaaggata ttatctatga ctgaacgccc
     6301 aataaaatat agtgcatccg ctgatcagtt acctgatatt gtcattagcg attatcctat
     6361 cgacaaaaag gcatatgatc aaacgaatac acagatattt tactggaatg gacaacctac
     6421 aaaaaatgat tgggcgcgtc tgaaagctga aatcataaaa atcaggaatg agaaaaatag
     6481 atccatattg gaaaaattgg atttcgttaa ttaagaaaag cagattgttt atgccaaaat
     6541 taagacattg caaaaagatt attttcttta aaatcgacaa gatgaccaga aagcaagagt
     6601 tcgttgagct cttgctttct tttagtttta gaaaggaaca tcctgatttt atcttcgcta
     6661 aatccagtca aaagttttgc agattggtcg tagataatcg gttttttcaa taaagaaggg
     6721 gatgtacgaa tctcaatata tagttcactc aaactgagtt catctattct gtgaacaaga
     6781 tttttataac taatagaatg agtagagagc atttcggtca cgccgttgtt tgtcaatttt
     6841 agtaagtgga taaattcatt tttggttagt ggttgacgaa ttaagttctg ttcgataaac
     6901 ggtatatcat ggacgataag ccaacgtttt gcgcgtcttg aagacgagca gccagaacct
     6961 gtatacaatt taatcatcat acattcctcc aatagctaag tatttatgaa ataataacat
     7021 gaagaaatag tcgaaaagtg ccatttagag gtgtaaaata agaaaatgtt gtcacgtata
     7081 atggaactag ctagataaga aatactatta aatcaagaaa aaagaaagaa ggcacaagta
     7141 tgtcaaacac agccgacgca atagtagtga tcgatttaca aaaaggggta tgttttgatt
     7201 cttcgccgat acacgaatta gaaacagtga cagcattagt gaataaaaga atcaaaacat
     7261 acgaaacgat tgggaaacca gttattttcg ttcaacacga agatgctcaa ttagtgccac
     7321 aaacaaaacc atgggagcta ttgtcggaat tatctattcc tgagcacagt tattttgtga
     7381 gaaaaaccca tgcgaattca ttttataaga ctaatttgag tgttttatta actgaattgg
     7441 actgtcattc gatcgaatta tgtggggcac aagcagagta ttgtatagat acgactgtga
     7501 agtttgctca tggattagga taccagctta tgatgtgtag aggagcaacg acaacttatg
     7561 ataatccttt tatgaaagca aaggaaacaa tcgcttttta cgagaaaatt tgggataaac
     7621 gatttctgac atttttggag caataagttg tttcttcgtt taaaaaaagc agaatggata
     7681 ctggtttatc cattctgctt tattacgtaa aatgagaaaa taatcctgtt ctatttttat
     7741 gggataaacc atttattttt tgtataaaaa atgtttgcgt ttacatataa ttctcctaga
     7801 aacataaaaa ggagtgaata gcttgagaac aaaacaagta ttaaagggag cgttggtcat
     7861 cttatttgct gtaagtacaa gcgtcccgca atggacagta tatggagaga gcatttcttc
     7921 cgaatcggaa tctcgggagg tagaaaccac agaaactact gatgctggaa atgaaacgaa
     7981 atcgagtact tcacaaacag aaacaaatac tgaaacaacg gttgagacat ctgttactag
     8041 cacagatcaa gaaacgaccg ctaccacaga taaccaacaa acagaagaag caacagctac
     8101 aaaaatgtca aacgacgaat cctcttctgc taaagtaagt gacaaagaga atgggacaga
     8161 aaatgatgat atcggtaaag gtgaaaattt caatggtaaa gaatatttga attacttcgg
     8221 taaacagatg acagaaatac ttaatggacc aacagaagac atcaagtcaa aattaataga
     8281 gttattagtc actgagcttg ctgttaatga ttatggtgta gataaagaga ttatcaaaca
     8341 tctgattgct acttatctat tgtacgatat cgcttttgca aaaaacagtg tgttgatgtc
     8401 gatgcttgga ggtacagatg atgggacaac gcctacagaa aacacaggct tctatccaaa
     8461 tacaaaacaa cttcatcaaa ctgtactaaa caaacaagat ggcaaaatga tggatgtata
     8521 tgcatattat gtcgatcaag gttcagataa aacagtcatg atccatggcg gatttcgagg
     8581 gaattggaat aacgggatcg ttacagaaga atacaatgat ttttataagg ccgggtataa
     8641 tttattattt gttgattcca gagcgactgg taatagcgga ggcgattatg tgacctatgg
     8701 acaatatgag tcagatgacg tcctttactg gataaatcaa gaagttcgag agcggccatc
     8761 tcaaaagatt ttactttatg gtggttcgat gggagcagct acgatgatgt ctgtattagc
     8821 gaaagatatt cctgtcaatg tcaaaggaat tattgaaaat tgcggatttg catcaattga
     8881 tgaacaacta cgttttacgt acagccagac agtagccccc gcactaccgg atgccataaa
     8941 aaatcagtta gacatcattg gggatcaaga gcatgaagat ttatttatgg gccttttgaa
     9001 acaatactat tttgatcagg agatgcattt ggatacaaaa gctgctctac ctacgattgg
     9061 aatgtcagat tcattgccta aattgattat ccatggaaca gcagatgatg tagtacctgt
     9121 gtctaacgcg caaaaattat atgagttatc aggagggtat aaagatcttt tattagtaga
     9181 aggagcaggg catggaaagg cacaggaagt tgaccatgct gcctacacga aacatgtaac
     9241 ggactttttg aaagtagtgt tccatgatca gataaacgta aagtatgtgg atgaaaacaa
     9301 taaaagtcta ctcaaagacc aggacgaaat ccagctttac ggagcttatg gagaaaatta
     9361 tatgacagag caaaagacat tcgaaggata cgaattagca aatgttgaag gaccgactga
     9421 agggattttt aatgagacga taccaactat tattttctat tacaaaaaaa tcccagtagc
     9481 tccaccaaaa aagcaagatc cgacagagaa tgcagacaat aagggaaaga atgaacctcg
     9541 agaggatcaa tcaagtgatg atacgcaacc gattcagatg acagtgaaag tacaaaaaaa
     9601 gacagatgca aaaaaaagaa tgttgccgaa aactggagaa aagaaacatt cgcttctcat
     9661 ttattcaggt ggatttttat ctgttagcac gattatcaca tggatttggc gaagaaagag
     9721 agggtaaatg aatgaaatgg ttcaagcgaa tagtgattgc agtaccagtt atcatcgtag
     9781 ttattttagt cactgtattt ttgattaatc aaatcacacc aaagccgatt agcttgcttg
     9841 ttagaaaaca atttgacaca agtgaaaaag aacaagtata tgcccgacca gatgatttcc
     9901 agcaaaaaat cgaacaaatc gatataaaaa aagaaatatc ctatccttca aagtacaaaa
     9961 ataataatat ggatatctac acaccgaaag tggaaaagaa aaaattaccg attcttttct
    10021 ggatgcatgg aggcgcatat gtaggagggg ataaaaatga ttgtcgtgat tatttggaat
    10081 atctatgttc agacacgcag caaatcatcg taaatataga ttatgaacga tcaccggaag
    10141 caaaacatcc tacaccagtt atccaattga acgaagccat tcaagcagct aaaaaagaga
    10201 taaaagatca agcagattgg tcgaatattt cgattggtgg aaattcggca ggtgcacaaa
    10261 ttagtggaga atatttgtta gcacttcaaa atgaagaaat taaaaaagct gatcattttg
    10321 atccgacttt ggagaacaag cagattacga agtttatttc tttgtctggt ctgttggatc
    10381 cttctgcact tactcaagta agtgataaaa tctctagctt tctttatgaa aaatgtggat
    10441 gggcttactt tgataataag gactttgaac aatcagagaa ggtcaaaagt ttagccttgg
    10501 tcagacatgc ggatcgttgg acgcaaaaca cattcttaac ggatggaaat acaaatacgt
    10561 ttacgaaaca aatggaagaa gtaacttctg tatttgagaa agcgggggtg aaagtcacaa
    10621 aagtagatta tgcggagaaa aatgctgttt tgaaccatga atatcagttt gatttcagca
    10681 ataaacaagc gcaagaaaca tatcaaaagt tagtaatgtt tttcaaggag taaagtctta
    10741 tgatgcaaaa gaggttgtaa tctgtcactt agctccaagt cacaaggagc aatttcaata
    10801 aacaagcttt tcatggttta tgaatattgt aacttgtgac tgaggagtta gagatggcaa
    10861 ggggcacctt ttattcgaaa tttgggaata tcacacgatt attgtcacga tctcttttac
    10921 gtaaatttta taagtcagat ttgttgtttt gatcgtttaa aaaaagcaac aaatccgaag
    10981 cctgcggagg caagaaggga caaaaagccg agtggtaagc aaaagaatgc tggttccact
    11041 aatattccgt tgttattgat ataaggctgg gaacaaagat gaatagcaaa taaaatcaat
    11101 gcactagcga aaaaaagcaa agaaatatgg atagctttca taagcagcac ctcctttttt
    11161 ataattgtat gataaataga ttaagaatct ataaatgtga caagtttgtt aggatgtgaa
    11221 taaaatgatc atctagcgaa cttgaaacaa agatggctaa tgataagcaa ttaatgattc
    11281 agcaatcaga taggtacaaa gtttaccatc atacgtctag accaataata gaaaatatgg
    11341 gaatataata gaaaagattt aggatacccc caaaagaatt tccccaaaaa ttcgatgtat
    11401 cctaaacttt tttattttac ataacaatca tatcaactgt gaaataaaat tacaaatgat
    11461 aacctaaaat gaggccaaaa gccagtgtta tcaaggaaat acttaattca ccaaaatttt
    11521 ttttaatttt ttagaaaata agaaacaaaa ataataaaaa aaagaaaaga taaatttttt
    11581 actactgtat actaactttg ttatcgcatc taacaagtaa ctcgaataat accaatacaa
    11641 ccttctccaa agatgcgatg acatacgttc taacccaccg actattgatg ttctgtacca
    11701 cccttgcaga accaaaaaag ccactaccaa atatttggta gtggcttttt ttgttttatg
    11761 gatttttcgc tttttctgtt atggtgctat tctttgattt cagaaatagt atgtcgaact
    11821 ttagcgatag aacgacgtga ttctttttcg ttgtaaaaca tcaaaatcac atatagagag
    11881 tccaaaatgc ttagttgaga aatacgggaa gctaaagctt cagatcggaa ttctgtttct
    11941 tcagaaatag aaataaaaac gacatcgcca agtttagcaa gggaagaatt agcttggcta
    12001 gtaataacga ttgtttttgc tccagttttt cttacggttt cagcaatatg gattgtttct
    12061 tttgattgcc ctgtgtgaga aatcaaaaca gcacagtctt tttccgtcaa taaagaagct
    12121 tccatcaatt gaatatggta atccgtgctg tgagaagttg cgatagggga acgcaaaaat
    12181 ttatgataag cgtcagttgc tacaatctct gaaccgccaa caccgaaaaa atagacacgt
    12241 ttagattcat tgataatagt agccgctttt tttagatcct tttgttgtaa caactttttt
    12301 gtgtcattta aagttgaaat actagactcg aacacctttt gcgccatagt gagctcagta
    12361 tctgttttct gaatattttc gtggatagaa atagcagata gatcattttc ttggatcaac
    12421 atggccattt taaaatcttt aaatccatta taaccaagtt tttttgtaaa ttgaaaaaaa
    12481 gtagagtcag caatgcccag ttcacttgct aaattgctaa taggactatg acttactttg
    12541 cttggatttt ctaaaatata atcggcgata gcctgttctt ttgggctgaa gtcgttgtac
    12601 attgttttga tttgtagtct tatggagacg ctcatgagaa ttcctcattt tcataataag
    12661 attatcattt tttccgttct cattaatgtt aaagatacaa ggagagtagg aaaaaatcaa
    12721 acaaaaaata tttttgtaaa atctatttac aaaaaaattt ttttgtataa tgagggtata
    12781 agaaatggag gcgctatcat ggaaattggt gtaattggtt tgggaaaaat gggattgaat
    12841 ttagctttaa acttaaaaga gcaccatcac aaagtacaag gactcgacat ttcaagtaca
    12901 gctgttagtc aggcaagaga aaatgacatt cctgcttatc aagagatctc tgaatttcta
    12961 gctacttttg agcagaaaaa aatcatctgg ttaatgcttc ctgctggaga gataaccgaa
    13021 aacatgttaa gaaaactttt tccaaaactc ggaccaggag acattattat tgaaggcgga
    13081 aactcttatt ataaagattc aatccgtcga gcagaagagt ttcagaaaaa tgaaatcggt
    13141 tacttggatt gtgggacttc tggaggaatc gaaggagcac gacatcaagc ctgcttaatg
    13201 attggaggag ataaagaaac ttttctatcc gtggaacaat tattcaaaga tgtagcaatt
    13261 gaaagaggat atctgtatgc tggtgcatcc ggaagcggac acttcttgaa aatgatccat
    13321 aatggaatcg aatatggaat gatgcaagca atcggtgaag gattccaact tgtacaaaaa
    13381 agtgagtatg cttttgactt ggaacaagtc gcacgtgttt ggaatcatgg ttcggtgatt
    13441 cgcggctggc tgatggaaat catggaagat cagttaaacg aacatcccaa tttaagtaat
    13501 tatcggggaa tcgtttctgc atcaggtgaa gcaaaatgga cggttgaaac agcgctagaa
    13561 atggctgttc cagttccagt aattgcaact agtttgttca tgaggaatct ttcacaggaa
    13621 gaagatagtt tttcagcaaa agtagtttca gcactaagaa atgggtttgg cggacatgaa
    13681 atagttgaaa agggtgagta aaatgccaga ttatcatata gcaatcgtta attccagcag
    13741 tttcggaaga gtctttccac accacttgga aagattaaag tcagttggag acgttcattt
    13801 ctttactgtt gatcaagaga ttccaggaaa agaactagcg gagttactta aaggattcaa
    13861 tatcattatt gcaagtgtta cgccattttt tcgacgagat ttttttgaaa acaaagatga
    13921 actgctattg atttcccgac atggtattgg ttacaacaat attgatttaa aagcagcaaa
    13981 agaacaccag acaatcgtat ctatcatacc tgctttagtt gaacgaaatg ctgtagcaga
    14041 aaataatata actaatttgt tgtccatgct tcgacgcacg acacaagcac aagcaagagt
    14101 caaggacgat cgatgggaag atcgggcgga gttcgtcggt aggacgctgt ttaacaaaac
    14161 agttggtgtg attggtgcag gaaataccgg aagcggagta atcgaaattt tgcgtaacgg
    14221 ttttcgctgt gatgttctag cttatgatcc ttacaaatca gatttataca tcgaaagctt
    14281 tggtgcaaaa aaagtagaac ttcaagaatt actggaacaa tcagatatca tttgtttatg
    14341 tgcgaatttg acagaagaga actatcacat gatttctgat aaagaagtcg atcagatgaa
    14401 agaaaatgtt tatctatcta atagtgcaag aggcgctcta gttgatgaag aagcgatgat
    14461 taatggtctg aaaagcggaa agatcgcagg atatgccaca gatgttttag aagaagaacc
    14521 gggacgtcaa gatcatccgt atttagcgtt tgaacaagta gtgattacgc cacatatatc
    14581 ggcctacaca agggaatgtt tagaagcaat gggtgaaaaa tgtgtcagag atgtcgaaca
    14641 gatcagtcag caccttcttc ctgaacgttc tgtccagaca aagagcaact atataaaagg
    14701 atgatgaaga aaatggtgaa cggtttaaaa gtgaaaacag gccctcaatt ttatttatat
    14761 gaagaaggtg gtatttcaaa agtttctgat ctcttgaaat catacggcgc gaaaagagta
    14821 ctggtcaccc acggaacggt atcatgggaa aaggcgctac ctaaattagt atttttgaat
    14881 gacgaaacga ttcaattttt ttatcatcgt tattctggag aatgtagtta tgctgaagcc
    14941 agacgaatag ctacaatcat caaaaaaatg aaatcgattt cgtagttggc gttggcggag
    15001 gaaaactgac cgatctagtg ggttatagtg ccaatcttgc aagaatcgat ttcggagtca
    15061 tccctacttt agctagtaat tgtgctccat ggacacctct tgctgtcatg tataaggaga
    15121 atggacaagc agaagggaaa acagaacact tttacagaca agctgctttt tttataacag
    15181 atccccaatt agtgattgat tcccctgttc catatttcat tgccgggtta gcagatacac
    15241 tagcaaaatg gtatgaatct gagctcatct tagaacaaga ttttttgcaa gatgaatcat
    15301 ttttgaagct agcacaagat actgcgaaga tctgcaaaga agagattttg aatcattctg
    15361 ctaaagcaat ccaagatatg gcacaaagaa aactgacatc ggagttcaaa cagctttcag
    15421 agattatctt tgctgttgct ggtttagttg gaggattagg agataaatac gcaagaaatg
    15481 tggcagcaca tgctatgcat gacgctatca gtaaatatat tccggaaagc catcgttttc
    15541 tacatggaga aaaagtagcg tatgggattt tctatcaact ggcactagaa ggtcgctggg
    15601 aaaaaataga cgagttatat cctttgtatc gggaattaga actgccggtt tctctagaac
    15661 aaatgggatt atttccgaaa gataaacaga taatagataa aatggctgaa tttattgatt
    15721 cgaaagagaa agtgcatttg attcctatag aggtaacaca gaaatcactg atacagtcaa
    15781 tgatggaact ggaaggttat atcagaaaga tctgaaggag gaggacacat ggaaacatta
    15841 agtgtagtac aaagtttgtt gattgcttta tgggtagccg cggttatgtc ccgctggtta
    15901 ggtggaggag ccacgttgac cttacgtttt tctcctttga tgactggatt agtcgtcggg
    15961 ttgatcatgg gggaagttgc acaagcgatg attgttacag ccgctttgca aatgatttat
    16021 atgggggttt tttctccagg aggatcaatg ccagctgaac catctattgc agcagctatc
    16081 gcagtaccag tcgcattatt aggggatctg tcgccagaag cagcgattgc cgttgcggtg
    16141 ccagtcggat tgctcggaag ttacttgtat cagtttcgtt ttttcatcaa tactttttta
    16201 ggtaaatata cagacaaagc tgtagaagat ttgaatacaa aagctatggc ccgatcaatt
    16261 attctttatc caacggttgc ctcattttta ttattcgttc cgcttgtttt cattgcttta
    16321 tatttagggg cgccattgat cgctgatgta atcaaagctt tagagggaac agtcgttatc
    16381 cacattcttg aagtagtagg tggagggtta gcggcaatcg gtatcgcgac aactgtatac
    16441 gttatcggtc gaaaagatta tctggtcttt ttcttccttg cttattttat gagtgttgtt
    16501 ttgaaatctt tagatattac gatggttact tatgccattt ttgggattat tatcgctttg
    16561 atctttgtcc agtcgcaaaa agggaaacaa gtacagcctg ccacaagcaa taacaattca
    16621 gcatcttttg atgacgaaga tgactatgat gacggatttt aatttaggag gaatgggaaa
    16681 tggctgaaat aatggataat caaacaggtg ctcctgaaga aatcacgaaa aaagatgtaa
    16741 caaaagccta tctgagatgg cattttgcaa acgaaattcc tcactctttt gaacgttact
    16801 tggcgccatc actactatac ggaatgatgc caatcttgaa aaaactttat aaagatgaag
    16861 actctttaaa agcagcatat aaacggcagt tactgttctt caacactcaa ttaagctggg
    16921 gtggcggtgt gattactggc ctaatgtctt ctatggaaca gcaaagagca gaagaagagt
    16981 atgaagaaaa agagatcttg atgcaggatg acttgatgta taacacgaaa gcgggattga
    17041 tgggggcact agcaggaatc ggagatgcaa tcgattcagg aaccgtccaa tatattttta
    17101 tcgcaatcgc ggtaccttgg gcacaacagg gaagtccttt aggcgcactt tttcctttta
    17161 tctgctttgg gttgtatcag gttttactag gggtattttt cgcaagacaa gctttcacta
    17221 tgggccgtaa tgctacaggc ctaaagcaaa acactggtgt tcagacagcg attgaaatgc
    17281 tatcagtttt agggttgttt atgatggggg tgcttgcggg aaactatgta aaagtagaat
    17341 cttcattgaa atttgcgata tccggacgtg agtttgttat ccaggaaatt cttgatcaaa
    17401 tcgtgccagg gatattacct ttagctgtcg taatgggtgt gtacttgttc tatacgaaaa
    17461 aaggattgaa agtcacacaa gcattattat ggctgactgg aatattaatt gtgctagcag
    17521 gaatcggtat tttataaaaa gaagggaacg tgaataagat gggtgtagtc aatttagcgc
    17581 gtgtggatga gcgtttgatc catgggcaag taatggtaac gttatctcaa aaaagtggag
    17641 tgaattccat ttttgttgta gacgaagttg tagcaaaaga taaatttatg agagatcttt
    17701 acaaaaatgc aggaagccgt acaggacaaa aaacaattgt cattacacca gagaaagcta
    17761 aattctattg ggatgaatat caatttaaag aatacaattg tattttgatc gcaaaaacag
    17821 tccaaacgat ttacgactta gtaaaacacg gaatccccat ggaagaattg aatattggag
    17881 ggattgctca aaaaaatcct gatacagatc ttttagtgac gaaatctgtt tatttaaata
    17941 aatcagatgc tgaaaaattg aaagaaatga atgaacaata tggcgtaaag aatatttatt
    18001 ttcaagccac tccatctgca cctaaaacag gattaaaaga tgtattgaac aagtttgatt
    18061 tgtcataaac aaatgcttag aaaaaagata aagagggcga aaaaatgaag aagaacgagc
    18121 tgtttcgtga ttgggaattt cgttatcgct atatttaccg taagcgccgt acaaaaaaga
    18181 gtaaacagcg ttttctttca gcattagtat cagatattta cagtatgaga acagatgtga
    18241 cagtcattgc ttacgatacg ctagcctacc gatcaaaaaa tatttatgtt ggagatatcg
    18301 aaaaagcaga gaaagttatc tgtacttatt acgatacacc cgttcatgca ttaggaagtt
    18361 atttcatgtt tgattggaaa gaccaaagga aaaaaacaat ctactctata ttattgtcct
    18421 tcattttgtt gttcagttta ggttggtggg gaatgatgat atataacaaa aatcctcatc
    18481 acgtattcga tctgctatcc gtataaacag gtatcactgt actagcattt gggagctact
    18541 ttttcttatt gggaaaagca gctcgcggat ggcctagcag acaaactttt attcgaaata
    18601 cttcatccat attaacgatg ctagaaatga tccgtacgat tgatgatccg agtgtagcgt
    18661 atgcgtttgt tgatgaaggc tgttatggag aaaagggctt agacagcgtg agatcgggta
    18721 tgaaaaaaga ggctattctt ttttatctag actctgttgg ggcagataca ccgctacagt
    18781 tttcgggaaa ttatttttca aataaagaac agtggctgaa acaagttgat aagttgaaag
    18841 aaaaaaacgt caattatatt tttagtgcaa gaaaaaagca agcgcagttc ttctatttga
    18901 ccaaaacaga tttacgaggg aaaacattta attggcaaaa tgccaatcag attattgctc
    18961 tttttcgata agtaaaaagt gcaaaacaag gagtgagaag actaatgttg gaaatcatga
    19021 tcgttacaca tggtaagttg agcgaaggac tgaaagatgc ggcggaagta attgtcggga
    19081 catcgagcca tattgaaaca atgtgcttag agtctgggga ggacatccaa acattcggag
    19141 agaaggtcaa agaaaaatta actatgatca acacagcaga acccatgcta gtatttgttg
    19201 atctaattag cgcaagtccg tacaatcaag cgctcttggc agtcaatgaa ctgactgaag
    19261 aaaagaaagc agccacttat gtcatagggg gcgtgaatct gccaatgttg ttagaagcaa
    19321 tcaatcatca gatgcttcac acaccgatag aagaagcggt ggaacaaatt gtcaaacaag
    19381 gaaaagataa tttaactgtt tggcatgctt cagcggaaga aaaaaaatta gatgaggacg
    19441 atttttagaa ggaaggctga aaagatgaag tggggattta gatggtatgg tgaaaaaggg
    19501 gattcgatcc cattaaaata tgttcgccaa ataccgggaa taaatgggat aattggaact
    19561 ttgctgacta aaatgcctgg agatgtttgg gagatttcag aaattgagga gttgaaagca
    19621 tctgttgaga gccaaggact ggaactgctt ggaattgaaa gtgtagcgat ccacgatgca
    19681 atcaaggcag ggacagatga acgagattac tatattgata actatatacg aacaatcaaa
    19741 aacttgtctc gatgtggggt tcgattgatt tgttatagct tcaaaccaat ttttggatgg
    19801 gcgaaaactg atttgtttaa tgaaaacaac gatggaagct attctctttt atacgatcag
    19861 aaaatcgtcg atcagatgga gccaggagaa atgtatcaat tgatacacag ccagtcaaag
    19921 ggattcaaat tgccgggttg ggaagaagaa cggttgaaaa aattcgctca gctggaaaaa
    19981 ctgtatgaag gaatgaccga agaagaatta tttgaaaatc tgacttattt tttagaaaaa
    20041 gtgattccag tgtgcgaaaa agcagatgta aaaatggcta tccatccaga tgatccgcca
    20101 tgggaacttt ttggttttcc tagagtgacg aaaaatcttg gcgacttgaa gaaaattcta
    20161 aatgcagtag attctccata taatggacta acgctgtgca caggatcttt aggggcaaat
    20221 ccggaaaacg atttagttga aatcatccat gaggtaggtt cgcgcatcca ttttgtccac
    20281 ttcagaaacg tggagttttt aggagaaaga aagttcaaag aaacggcaca tccctctaca
    20341 gaaggctccc ttgatatgta tcatattatg aaagcgttgg tgcaagaagg gttcgacggt
    20401 cctatacgcc ctgatcatgg tagaaccgtg tggggtgaag tggctatgcc tggatatggc
    20461 ctgtatgatc gtgcaatggg actcacttac atgcaaggtc tatatgaggc gataagtaag
    20521 gaaaaaccac tgggagagca ttatgaataa aatcaagctc ttcatctttg atatggatgg
    20581 ccttttatta gaaactggac gtttagctta tcgtgcttat gtaaaatcag cacaaaagta
    20641 tgattatgaa atgcgtaagg aagtctatta tttactaact ggccaaacag aaatggcgat
    20701 aagagaacaa atgggcattt tgtacggaga agatgttcca tatatcaaat ggcgagaagc
    20761 aatcaatcaa tataaagaaa aaatcgtgaa agaagataag agagtatata cgaaaaaagg
    20821 agcagaagaa attttatctt ttgcaaaaga aagaggaatc catacaatcg tggcttcgtc
    20881 taatacacgg gtaaaagtag aaatgtattt aaaaatggaa aatctatatg ccttgtttga
    20941 tcaaatcatt tcaggggatg atgtaaaaaa aggaaagccg gaaccagaga tctttttgaa
    21001 agcttgttca aaaatgaata ttcctccaag tgaagcactt gtatttgaag actcgattgc
    21061 agggatcgaa gcagcaaggc gagcgggcat tttgtctttt ctcatcaaag atcatttgat
    21121 agggttgccc gatcataatg ggaagcataa gttgaaagta gatgtaagtt tatatgaatc
    21181 caataaagca gactatgaat tttatgacct gcatcaagca aaacgatttt tagaaagtaa
    21241 agggttgtat ctctaataaa gaggactatt ttctagtcct ctttatttat tgtcctgcac
    21301 gactgaggga acagatgaaa aagaagtaat tttttatttt gaagtatcta ccttatttgt
    21361 agtgaacaat ttagaacaaa cacaaaacgc atctactgtt atctaccagt agatgcgttt
    21421 aaagagaaga acgagaatcg tccatatgag ggtttccact ccttatgcag atttttttaa
    21481 ttcttgcact gcttgttcga tcgtaacacc tgtcgctaca tgattggtat tttctttgtc
    21541 aaaaacgaca aatagattca aatctgtatc caaggttact cgatattgta tatctttgtt
    21601 atagaatgtc atagccattc tcctttcttt aaatacttgc tgaaaggcaa cgaacgcgtt
    21661 gataacgaaa ttccatttat aagtatacca ccaattgaaa ggttttcata gttaagaaat
    21721 tttaaagtca aaagttgcag ataaaagcaa ataaacaaga aaacaaaaag tgaatcaatc
    21781 gcggttattc aactttttct tcgattcgaa gacaatagaa gacaatgaat tttatatttc
    21841 attgcattat ccttgtgtta ttagaaaaag catgatatat taaatgagta atctattttg
    21901 aaacgtaatt tgagcgatat atctacatag ataaaactcc ttttgcctat caaatattta
    21961 ttgcaacgaa aaaacgtaca ttgatacgta tcaggaggaa tttatttatg aataacggta
    22021 cagtaaaatg gttcaacgca gacaaaggtt ttggctttat cactggtgaa gatggaaatg
    22081 atgtatttgc acatttttca gctatccaag gtgacggctt caaaacttta gaagaaggcc
    22141 aagcagtgtc ttacgatgtt gaagatggtc aacgtggccc tcaagcaaca aacattgtta
    22201 aagcttaata aaaattgact tttaaccgtc ttttcgaaag aaaa
//
LOCUS       DACEDO010000047        21832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEDO010000047 DACEDO010000000
VERSION     DACEDO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.047"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO52_001861"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO52_001861"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0356127.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO52_001862"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO52_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0356128.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO52_001863"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO52_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0356129.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO52_001864"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO52_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0356130.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO52_001865"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO52_001865"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0356131.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO52_001866"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO52_001866"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0356132.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWO52_001867"
     CDS             complement(8053..8523)
                     /locus_tag="IWO52_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0356133.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWO52_001868"
     CDS             complement(8569..8994)
                     /locus_tag="IWO52_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0356134.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWO52_001869"
     CDS             complement(9061..9330)
                     /locus_tag="IWO52_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0356135.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWO52_001870"
     CDS             complement(9341..10936)
                     /locus_tag="IWO52_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0356136.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO52_001871"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO52_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0356137.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWO52_001872"
     CDS             complement(14489..15049)
                     /locus_tag="IWO52_001872"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0356138.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWO52_001873"
     CDS             15402..16376
                     /locus_tag="IWO52_001873"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0356139.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWO52_001874"
     CDS             16546..17946
                     /locus_tag="IWO52_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0356140.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWO52_001875"
     CDS             complement(18024..18746)
                     /locus_tag="IWO52_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0356141.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWO52_001876"
     CDS             complement(18825..19331)
                     /locus_tag="IWO52_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0356142.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWO52_001877"
     CDS             19454..21253
                     /locus_tag="IWO52_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0356143.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..>21832)
                     /locus_tag="IWO52_001878"
     CDS             complement(21321..>21832)
                     /locus_tag="IWO52_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0356144.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6048 a         4788 c         3964 g         7032 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tg
//
LOCUS       DACEDO010000048        21813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEDO010000048 DACEDO010000000
VERSION     DACEDO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.048"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            71..505
                     /locus_tag="IWO52_001879"
     CDS             71..505
                     /locus_tag="IWO52_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0356145.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(583..1116)
                     /locus_tag="IWO52_001880"
     CDS             complement(583..1116)
                     /locus_tag="IWO52_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0356146.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1210..2388)
                     /gene="mutY"
                     /locus_tag="IWO52_001881"
     CDS             complement(1210..2388)
                     /gene="mutY"
                     /locus_tag="IWO52_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0356147.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2381..3178)
                     /gene="recX"
                     /locus_tag="IWO52_001882"
     CDS             complement(2381..3178)
                     /gene="recX"
                     /locus_tag="IWO52_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0356148.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3285..4667
                     /gene="rlmD"
                     /locus_tag="IWO52_001883"
     CDS             3285..4667
                     /gene="rlmD"
                     /locus_tag="IWO52_001883"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0356149.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4841..5419)
                     /locus_tag="IWO52_001884"
     CDS             complement(4841..5419)
                     /locus_tag="IWO52_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0356150.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5741..6211
                     /locus_tag="IWO52_001885"
     CDS             5741..6211
                     /locus_tag="IWO52_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0356151.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6319..7545)
                     /locus_tag="IWO52_001886"
     CDS             complement(6319..7545)
                     /locus_tag="IWO52_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0356152.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8299..8499)
                     /locus_tag="IWO52_001887"
     CDS             complement(8299..8499)
                     /locus_tag="IWO52_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0356153.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8633..9241)
                     /locus_tag="IWO52_001888"
     CDS             complement(8633..9241)
                     /locus_tag="IWO52_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0356154.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9326..9796)
                     /locus_tag="IWO52_001889"
     CDS             complement(9326..9796)
                     /locus_tag="IWO52_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0356155.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9844..10758)
                     /locus_tag="IWO52_001890"
     CDS             complement(9844..10758)
                     /locus_tag="IWO52_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0356156.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10904..11707
                     /locus_tag="IWO52_001891"
     CDS             10904..11707
                     /locus_tag="IWO52_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0356157.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11885..12832
                     /locus_tag="IWO52_001892"
     CDS             11885..12832
                     /locus_tag="IWO52_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0356158.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12900..13193)
                     /locus_tag="IWO52_001893"
     CDS             complement(12900..13193)
                     /locus_tag="IWO52_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0356159.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13221..13886)
                     /locus_tag="IWO52_001894"
     CDS             complement(13221..13886)
                     /locus_tag="IWO52_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0356160.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14026..14658)
                     /locus_tag="IWO52_001895"
     CDS             complement(14026..14658)
                     /locus_tag="IWO52_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0356161.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14665..15732)
                     /locus_tag="IWO52_001896"
     CDS             complement(14665..15732)
                     /locus_tag="IWO52_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0356162.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15729..16460)
                     /locus_tag="IWO52_001897"
     CDS             complement(15729..16460)
                     /locus_tag="IWO52_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0356163.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16629..17108)
                     /gene="greA"
                     /locus_tag="IWO52_001898"
     CDS             complement(16629..17108)
                     /gene="greA"
                     /locus_tag="IWO52_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0356164.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17244..18419)
                     /gene="mltG"
                     /locus_tag="IWO52_001899"
     CDS             complement(17244..18419)
                     /gene="mltG"
                     /locus_tag="IWO52_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0356165.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18623..19792
                     /locus_tag="IWO52_001900"
     CDS             18623..19792
                     /locus_tag="IWO52_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0356166.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(20059..21681)
                     /locus_tag="IWO52_001901"
     CDS             complement(20059..21681)
                     /locus_tag="IWO52_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356167.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6750 a         4245 c         3449 g         7369 t
ORIGIN      
        1 ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg
       61 tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc
      121 tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat
      181 agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca
      241 aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat
      301 tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt
      361 tgctaagaat caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt
      421 ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca
      481 tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct
      541 tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg
      601 cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt
      661 gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta
      721 attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc
      781 aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa
      841 aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca ttgctattac
      901 attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg
      961 tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt
     1021 ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg
     1081 gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt
     1141 ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat
     1201 gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt
     1261 catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt
     1321 tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac
     1381 atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac
     1441 aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa
     1501 cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt
     1561 cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc
     1621 aaaataatac gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg
     1681 ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca
     1741 aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc
     1801 tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat
     1861 cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa
     1921 tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat
     1981 acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg
     2041 cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt
     2101 ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc
     2161 atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac
     2221 tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg
     2281 attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg
     2341 aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct
     2401 tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct
     2461 ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc
     2521 cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta
     2581 tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc
     2641 tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat
     2701 gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag
     2761 gtttcaaccc tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc
     2821 gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt
     2881 ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg
     2941 ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac
     3001 cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc
     3061 cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag
     3121 ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa
     3181 acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt
     3241 atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg
     3301 tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa
     3361 tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac
     3421 tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa
     3481 aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc
     3541 aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag
     3601 cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga
     3661 tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga
     3721 ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag
     3781 actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga
     3841 ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca
     3901 tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa
     3961 aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt
     4021 caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc
     4081 acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc
     4141 ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca
     4201 aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga
     4261 tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag
     4321 caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag
     4381 tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta
     4441 tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat
     4501 accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg
     4561 ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga
     4621 ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg
     4681 tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt
     4741 tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa
     4801 agggttgtga catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta
     4861 tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat
     4921 atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg
     4981 ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat
     5041 tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat
     5101 tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct
     5161 gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg
     5221 tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta
     5281 agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat
     5341 gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata
     5401 acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt
     5461 tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa
     5521 aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga
     5581 atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta
     5641 ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag
     5701 ccttcatggt aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat
     5761 tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag
     5821 cggaaatccc ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg
     5881 gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt
     5941 acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt
     6001 catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc
     6061 aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca
     6121 tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct
     6181 attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa
     6241 cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat
     6301 taaccaaact atttttttct agtacccact tgttgttcca tactcattat tctggtcata
     6361 accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg
     6421 tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg
     6481 atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc
     6541 accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt
     6601 tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg
     6661 gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc
     6721 ttcgccttgc aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac
     6781 atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat
     6841 ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac
     6901 ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga
     6961 ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc
     7021 caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc
     7081 catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat
     7141 tgaatccatg gccatttcga caccaccaaa tgcgtaggca tgatttaatt tatctaccgt
     7201 tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt
     7261 ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc
     7321 taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct
     7381 tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt
     7441 ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac
     7501 gaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt
     7561 cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca
     7621 taagaaaact ttaaaaaaca tttttgtgta acgattatta ttttttaaaa tttctcaaaa
     7681 ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca
     7741 atcgttttat ctttttttat gtattttttc cagaaaaagc gaacaacaag gtattttcca
     7801 aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct
     7861 accgttcaga ttatcattcg atttcacgac aatttcttcc tatgactggt ccttttgtta
     7921 aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac
     7981 tagtgattgc attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg
     8041 attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca
     8101 aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag
     8161 aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta
     8221 gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg
     8281 tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc
     8341 ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg
     8401 aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc
     8461 tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatatc ctccaaaaaa
     8521 gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt
     8581 cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg
     8641 ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat
     8701 aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta
     8761 gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta
     8821 attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt
     8881 ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta
     8941 tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt
     9001 tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca
     9061 tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg
     9121 aatttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt
     9181 cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca
     9241 taaaaaaatt cctcctcaat gttttcttaa taatgtctac attacaaaga gtaacaaaga
     9301 gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt
     9361 caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc gcaaggataa
     9421 caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca
     9481 ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc
     9541 attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg
     9601 ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag gccatactta
     9661 aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac
     9721 caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact
     9781 gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat catactctat
     9841 atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt
     9901 ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg
     9961 aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa
    10021 aataattatc ttttcgctat actgccaaat atggacatga tgaacatcct ttaccccatc
    10081 gattgcttca atttcttgtt ccattttttc atagtcgata gcaggtgcac tttgtatcaa
    10141 aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat
    10201 cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat
    10261 aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt
    10321 caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc
    10381 aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt
    10441 gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc
    10501 actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc
    10561 cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc
    10621 atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat
    10681 caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg
    10741 cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt
    10801 ttgatatttc accaatcatt cagctgtttc cttaaagcac ttgtatttca cttgatactt
    10861 cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt
    10921 aattatcagt tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg
    10981 gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca
    11041 agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta ctcgcattcc
    11101 tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca
    11161 aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc
    11221 tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc
    11281 tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta
    11341 caccaatgaa tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc
    11401 atctatacca ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct
    11461 gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg
    11521 actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc
    11581 tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat
    11641 tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat
    11701 caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt agaggttgtg
    11761 acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc
    11821 aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt
    11881 aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga
    11941 aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca
    12001 aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc
    12061 taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt
    12121 ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga
    12181 gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca
    12241 gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat
    12301 actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga
    12361 catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt
    12421 ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc
    12481 tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa
    12541 atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc
    12601 tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc
    12661 acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt
    12721 gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt
    12781 tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa
    12841 aacaatattt tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt
    12901 tattgttttt taaagtgata ggttggctcc tccaggttgg gatcataatc aacaagtaaa
    12961 tcatatcctt taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt
    13021 tccactttct ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca
    13081 tgaacctctg ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga
    13141 tcaatttctg agtcaaacca cttttgtgct tttggcgtta ccgttaattt catcccaaca
    13201 acctactttc tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct
    13261 gtctctccaa taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa
    13321 gcgataaccg tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg
    13381 aaatttaatt cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct
    13441 aaagaatatt tatctgacaa ctctaggtaa tctaaaatat tctttgacac taaattatgc
    13501 gcaatcctta ttcccatgtc tcgctcggga tggacgacgc gatcagctcc gatcttttca
    13561 agtactctgg catgatactc attttgggct tttgctaaaa tatttggtac gcccatttcc
    13621 tttaccatca gagtaactaa gatactggct tgaatatctt ctccaatagc gacaattaca
    13681 tgatcgaaat ttcttatgcc taatgatcga agtgtcattt catcctgtgc atttgccaca
    13741 acagcatgag tggctatatt catatattca ttgacccgat cttcattact atcaatagcc
    13801 agtacttctt gaccagcttc gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga
    13861 cctacaattg caaaattttg tttcaccgtt ttcctccttt agtatcttct tttctcagta
    13921 taacaaagcc tttacaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat
    13981 aatagttatt ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc
    14041 atgtttgaat gcatagatag cggcttgtgt acggtcttcc acttctagct tagccaatat
    14101 attggaaaca tgtgttttta ctgttttcag tgtaataaac aattcgtccg ctatttcctg
    14161 attacttttt ccttcagaaa tcagcattaa aatttcattt tcccgattcg ttagttcttc
    14221 atgtaataca ggctcatttc gccgactcat tttttccatc atttttgttg tcacttctgg
    14281 ttctaatact cgctcgccac gttgcgttgc ccttatagca tcagcaattt catgtgcggt
    14341 agaggtcttc aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc
    14401 atcaataaaa ctggttacga taatgatttt agcttctggc caatctttta agattgcttt
    14461 tgttgattcg ataccatcca tttcttccat gactaagtcc attaaaatga cgtccggtcg
    14521 aagtgtcatc gccttctcat agccttgtcg tccattttca gcttctccaa ttacttcaat
    14581 gtcttcttga atagataaat aagaagagac gcctaagcgc accatttcat ggtcatctac
    14641 taataaaact tttatcatcc ttatctatgc ctccttcatc aaagggacct tgatttctac
    14701 acttgtacct tgacctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg
    14761 ttctttgata ttgttcaatc cataacttcc cgttttcgtt tcattcatat taaaccctgt
    14821 tccgtcgtca ataatacgta acaaaagatt attgtctatt ttgtgcaaat atacctctaa
    14881 ttcattggct ttagcatgtc ttaatgtatt tgataacaat tcttgaacga ttcggaacaa
    14941 gtgatcctca atggaactag atagttttac atcttctaca tcccatttca gcgaaatctg
    15001 gattttattt tgcaattcct tcaataactg ctcgatacct tgctttaaac ttttctcttc
    15061 taaattgact ggacgtagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat
    15121 gatctctgct accattttca actgcttgcg aaacatttct ggtgtctcgc ttttttctgc
    15181 ttgctccgtc aatgccgaca tcatcatcat tgctgcaaac aattgttgcg aaactgaatc
    15241 atgcagctca cgggctaatc gatgtctttc taattctaaa atttcttctt tggtttgtcc
    15301 atctacatat tggggacggc tcgttacaat ttgtaattca ctagatactt caatcatttt
    15361 ttcttttatc ttagtgattt ccttatcaat atctttgatg tataaatcgt ccgatgcact
    15421 tgggatcgga agaagcaaca atttggattc ataattgcca gaagaaagtg cacgcagctt
    15481 ttcttcgatt ttcccgtatt ttgtcttttg aataagtgat aataatagaa agacaattaa
    15541 tcctacacct aaggaaatgg ctaacaaatg gaagataagc ggaacatata ataaacgtgc
    15601 acgcagtaat tctccccacc aatgactttg attgctggca taaaaataag tgaaaagtga
    15661 aaatagaata ataaggaaag ccgcaatccc cgaataaaca gctagcatcg ctctggatat
    15721 ttttcccatc atattcgtat cacctcgaca tctccaatca aggtattagt aataatcttc
    15781 aatctacgag ggttttcatc ataatcactg ctgtagattt tgatttgttc atttttcaaa
    15841 gaaaattgct cttcttcaaa caagacattt cctactaatg tcgcatgctc taatcggatg
    15901 gctacgccca aaggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata
    15961 ttatcatctt ttggcaaaag cgtattgccc aaatctatga tagtatcacc agaaatgacg
    16021 gcgatgttga tatcgtccca ttcatagacg tcgtttccaa tacgttggtt accgaataat
    16081 tgctgtcttt tccgctcatt attatgtgtt tttaattgct ctgtttggat catcatgatc
    16141 tgtttctttc gccaaaaagc atttttggtt aaatctatcc ctgaaatctc tacacctttc
    16201 agaccaataa ataaaacggc aaatacagcc atcatccata aagcaggact gttgactaag
    16261 ctaaaaaaaa tgaccaaact tcctaatatc aattgaaaat tttgaaattt agtacgagga
    16321 tgtttgcgca tagctagata tatattgaaa ataccgaata tcactaaaag caaaagtcca
    16381 gtattgttta cgatttgcca aacagcaaat aacagcagta gtgcttcaac taccacaaaa
    16441 aaacgccaag aactattcat cgctctttct ccctcctgac acattctcat ttcatcatac
    16501 ctattttttt aactgttggc tataggactt tagatgtaag atcgtttgtt gaaatctaaa
    16561 atacacaaag agaaaaagat ctagagtgta tcatcagtaa gatgaactct ctagatcttt
    16621 cagtcagact acgcttgttc tactttgacg attttaacta acatatcgcc acctggagta
    16681 gcaatagtta cttcatcatt tttacgtttg ccaatcaatg cttgcgcaat aggagaatca
    16741 ttggaaattt tgccagaaaa cggatctgct tcagcgcttc cgacaatcgt gtactcttct
    16801 tcttctccat ctggtagttc gatgaatgta actgttttac caattgatac ctcatctgca
    16861 tctacaccgt cgttatcgat gatttgagca aaacgaatca tattttcaat tgttgtgatt
    16921 cttccttcaa caaatgcttg ttcatctttc gctgattcat attcagagtt ttcggaaaga
    16981 tctccgaaac cgcgggcaat tttgattcgt tcaatgattt ctccgcgttt gactgttttt
    17041 agttcttcta attctttttc taatttttct ttaccttcta gagtcattgg ataaactttc
    17101 tctaccatgt gtaacgtctc cttttttcga taaaataccg caaaaggtaa tcttttgagg
    17161 aatatagtcc aaaaaagatt accacgcttt tcttcaaatg taaatgtttt tctaaaaatg
    17221 atcaatgtct agagcctgtt tttctaagaa ttatttacat atttttccac taattccata
    17281 tgttcttcat aagttttagc gaaataaaca tttccagtgc tcgtatctgc tacgaaataa
    17341 taatagtcat tttctgctgg ttctaatact gccttgattg cctcttcgct tggattgtca
    17401 aaaggtccag gtccatagcc ggtattggta tacaaattat atggtgaatc tacagctgta
    17461 tcttcatacg tcactaattc tttgtgttca cctaatgcgt ataaaacaga gatatctgac
    17521 tgaagtggca tatttgcttt gattcggtta aagaagactt gtgcaatgtt tttacgatca
    17581 ttttctttta cgccttcttt ttcaactaga gaagctagag ttaaaacttc ttgtacagtc
    17641 aaatttttct gcttgatagc tgaataatag ttttgcatga ccgtattcgt cttgttcacc
    17701 atttcaatca ctagatcctt aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat
    17761 aagtaacctt ccaaacggta ttttgtgttc tcagctttag atgcactttc caacagttct
    17821 gggaattttt ggtgcaactc gttaaagaaa gtctcatcat tcactaaatc taaaaattct
    17881 ttcttgtctt taccagttac cttcgctacc cgttcagcaa tctgatcgat atcgtaccct
    17941 tctggaatgg caattttccc atctgcaact tttgttggct cagaagtacc gccttcttgc
    18001 aactgtttgc caatctcgtc aagagtcata ttaggcgcta actgataata gccagcttga
    18061 aagccagtta aatttttaaa ttttgtataa taattgaaaa caatcccgct tttaataata
    18121 ttgtccttct ccaaaatctc accaatctgc ttgttagaag agccactcgg gatctcgact
    18181 tggactaatt ggtcatctga tttatctaaa ggcttcaacc cgctatctac ataacgatac
    18241 acggtaaatc caagaaatcc gccaataatc aataatgtaa gagcaatcac taaaatgatc
    18301 ttcctcacaa tcttatcttc ttttttacgc agatttctcc gcttgtcgat tatatctgct
    18361 gtagctgcct tactggcttc ttttttatct ttacttgggt gttgtttgtt tcctgacaaa
    18421 acaactcctc cttactatca agttcgtaac tttattcatt atacatgata tttgaattgg
    18481 cgtgaatgat tttatttaaa tattctatca aaaatgtaag tagtaaatcc attactataa
    18541 gattaaaata aatgttatca agatacaagc tattttagct ttattctttc tgtaaaaaag
    18601 aaaggagact aaataaacta ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc
    18661 atttttaatg atcgttgtcg tgttgtttag ccttagtgcg ttctctacag gaactattct
    18721 tggatggcaa ttttacaata atgaacactc tcagatagtt gcccatgctc aatcagctca
    18781 aaaaaagttg atactgacta ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt
    18841 aagaaaaaat gattatattg gctcaattta cgtaaagaaa aataatcgca tcatcatgga
    18901 aaaaggctat ggctatgcga ataaactcac gcaacaacca aatgatcctt ctctttatta
    18961 tcagattggt tcggttcaaa aagcattgac tactcttttg attttaaaac aagtcgagcg
    19021 tgggcacctt tctttagata ctaagctggc tcaattctat ccacaaattg ctggcagcca
    19081 gcaaattact atccgtgact tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc
    19141 aaccataccg atgactgatg aagaagtcgt tcaatttgcg attcagcact taaaatttac
    19201 agattatcag acctctcatt atgattcatt gaactttacc atattgactg gaattttgat
    19261 caaattaaca aatcaaccgt atgaaaaatt attgaccgat gatatcatcg aacctttagg
    19321 attacagcat acaaaatttt atgaccaagt caaaaattca tccaatcatg cactatccta
    19381 tcaaatgtct aaagaggatg attactttaa accactgaca gaatctgaaa cagagatcca
    19441 taacgaatta gggactggta atatcagtat gtccgtctat gatttaaata cgctgtttac
    19501 acaagtttta tctggaaaac tagtttccaa agacttactc ttctcattat ggcgtgaaaa
    19561 tgcgaatgga catccatata gtggtggggt atatagcgga gataattata tcctgtcgca
    19621 aggaaatatc gactcttttg atacatctgc tatatttagc aaagattcac aggatgctat
    19681 catcatggaa agcaatataa aagctgacaa aactatcaaa ctgcctactg ctgatctaag
    19741 aaaccaaatc tatgacctga tggaaggaac tactaagttg gcaaatagct gatatgatga
    19801 aaagatagca aatactactt ctttgaatag catttgctta aaaaaagtac tcgactaaac
    19861 gtaggaaaga gtgtgggaca aatctaaaaa tcaatttttg ctacagactt taattccaaa
    19921 taaacagaac agaaagaggt gttcttcttt ctgttctgtc caaattattc taagatttct
    19981 ctttaccaac attatttgac aaagggccta ttaaatctaa aatagtatct ttagattgct
    20041 aaaatatgtg ctagcaaatc attcatggct tgttcttatt tttaatttta tgcaataaaa
    20101 aagaagaaca attagcgtga ttatagaaat aaataatgag attttagaaa aaaatgtgta
    20161 tgaatacctg attgttactt tatttattcc actttcactt tttactgtaa caaaaggacc
    20221 tccttgtttt agtactgtag cattttttga attaacagta accgttaatc ctttataccg
    20281 gaatacaggt aaaactattg ttttgccttt tgaagattta aacgtaaatg taatttcatc
    20341 tttatttgga gaaaagctac tataaataac ttgaccatct tctgtaactt gatggttttt
    20401 caaaagcaat tgatcttttt tatagcctat aggtacataa tcaaaattct caaattcttc
    20461 aagtttattt tccatttgtt cattagaata agttccttga aaattcattt ctttcaccat
    20521 gttaactact gatgaataat ttaaaattga aatagctaat attaataata gtataaaatt
    20581 tcttctcctt gtataagaaa atttttccaa ctgtggagaa ataaatagag atactgaaaa
    20641 agatatgaac aatgttgaaa atatcataaa acgccaagga gcttgaataa tatttaaagg
    20701 agtattttgt aaggcaaacc atggaaatat ttttgtacaa agtagaatag atattatacc
    20761 cattatataa gtgattttag ataagattcc taatttttta aagttaaatg ttagtataat
    20821 taaacaaata agcaaagtta agccaatacc aaatgatagt aattctgaat ctatacttcc
    20881 aatcaaaaac tctttaccca gataaattgc tcttttttgt aatatatcca cataaggtct
    20941 attcagatct tgatacaatg tctgctcaat cataggacct ataaacgcta gtgaaagaag
    21001 taaagtaact atagcagctt ttattaattt aataattaag aagatttcta ttttcttttt
    21061 ccgataaaat tgaataatta aagcaattat aatcattacc atagtcatag ctacagaaag
    21121 aacatgagtg taaattaata aagtcatgct aatcgccaac ataacccaag attctttttt
    21181 tgagtcgtaa agctttataa acccgagtaa aataagagga agtattgaca ttgcgagtaa
    21241 ttcccccatt gcccctcgaa gaaatatttc tacactccta aaagaagatg ttgtatatat
    21301 aatagcgaaa aaggagctag taacatgttt tttggtaatt tcatacatta cataatgaca
    21361 tataaataga gtaatgagcg taacaatata aagaaaaagg ttatagccta ttgctaaatt
    21421 agtaaacttg atcaataaaa ataaaggata tagagttaac catgggtaaa aattatttac
    21481 ttgcatgcca tatgatctaa acgtttcaaa attaataggg gagctaaaaa tagattctaa
    21541 acttaacgca cgattaatgt gaaatgccgt atcataaaag ccaaatattt ccatgttttt
    21601 aaatgctgga aataaatata ataaactaac aaatgcataa actataaata tttttacaga
    21661 aaaaattttt ttcaaattca tataccacct catgtttttt tatattacat atagtatcat
    21721 ttattttttt aaaacactag attagtcact atataaaatg tgattaattt tattattaat
    21781 aaaatatttt ttatattaca aaagtagtgt ttc
//
LOCUS       DACEDO010000049        21801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEDO010000049 DACEDO010000000
VERSION     DACEDO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.049"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(153..923)
                     /locus_tag="IWO52_001902"
     CDS             complement(153..923)
                     /locus_tag="IWO52_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0356168.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(916..1851)
                     /locus_tag="IWO52_001903"
     CDS             complement(916..1851)
                     /locus_tag="IWO52_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356169.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2167..3816)
                     /locus_tag="IWO52_001904"
     CDS             complement(2167..3816)
                     /locus_tag="IWO52_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0356170.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(3933..6536)
                     /locus_tag="IWO52_001905"
     CDS             complement(3933..6536)
                     /locus_tag="IWO52_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0356171.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(6548..7597)
                     /locus_tag="IWO52_001906"
     CDS             complement(6548..7597)
                     /locus_tag="IWO52_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAQ0356172.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(7623..7937)
                     /locus_tag="IWO52_001907"
     CDS             complement(7623..7937)
                     /locus_tag="IWO52_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0356173.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(7953..8399)
                     /locus_tag="IWO52_001908"
     CDS             complement(7953..8399)
                     /locus_tag="IWO52_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0356174.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(8400..10376)
                     /locus_tag="IWO52_001909"
     CDS             complement(8400..10376)
                     /locus_tag="IWO52_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ0356175.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(10534..11430)
                     /locus_tag="IWO52_001910"
     CDS             complement(10534..11430)
                     /locus_tag="IWO52_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0356176.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(11423..12103)
                     /locus_tag="IWO52_001911"
     CDS             complement(11423..12103)
                     /locus_tag="IWO52_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0356177.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(12104..12886)
                     /locus_tag="IWO52_001912"
     CDS             complement(12104..12886)
                     /locus_tag="IWO52_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0356178.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12879..13796)
                     /locus_tag="IWO52_001913"
     CDS             complement(12879..13796)
                     /locus_tag="IWO52_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0356179.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13960..14283)
                     /locus_tag="IWO52_001914"
     CDS             complement(13960..14283)
                     /locus_tag="IWO52_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0356180.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            15071..18298
                     /gene="fss3"
                     /locus_tag="IWO52_001915"
     CDS             15071..18298
                     /gene="fss3"
                     /locus_tag="IWO52_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0356181.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18411..18725
                     /locus_tag="IWO52_001916"
     CDS             18411..18725
                     /locus_tag="IWO52_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0356182.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18738..19112
                     /locus_tag="IWO52_001917"
     CDS             18738..19112
                     /locus_tag="IWO52_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0356183.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            19122..19457
                     /locus_tag="IWO52_001918"
     CDS             19122..19457
                     /locus_tag="IWO52_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0356184.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            19540..20880
                     /locus_tag="IWO52_001919"
     CDS             19540..20880
                     /locus_tag="IWO52_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0356185.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            20958..21632
                     /locus_tag="IWO52_001920"
     CDS             20958..21632
                     /locus_tag="IWO52_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0356186.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
BASE COUNT         6778 a         4258 c         3902 g         6863 t
ORIGIN      
        1 atgcagaaca gctttttcac aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg
       61 tattagctca acaaacgaca aaatatgagg aactattttc ccgtttgttt ctctaatact
      121 caggtcaaaa cgctgatttc acaacctctt ttctaacttc tcaatcctct tcctttattg
      181 atcaaatacc aagtaacggc gtacaaaaca acgatgaata gcacaagtac gatcatcgat
      241 gttgctactg gcacatcgat tgttccaagg aatccgtaac ggaaaccgaa gatcatataa
      301 acgatcggat tgactttcga aacagcttgc caaaatggcg gcaacataga gatggcatag
      361 aaaacgccac ctagataagt taacggctgc aagacaaatg taggcacgat cgatacatcg
      421 tcataagact gagcaaaaat cccgttgagc agtcctgcta acgaaaacaa gattgccgtc
      481 atcaataatg taatgatgac catgaaccat gaatacacat gaagtggtac aaagaacaag
      541 gaaatgatcg ttacgagaga gccgacgaga atactgcgac ccactccgcc aatcacaaag
      601 ccccaaatga tcacatgcgt aggaacaggc gctaccaaga tttcttcgat gtttttctgg
      661 aatttttgag agaagaagga cgaagaaacg ttggcatacg aactagtgat agcagacatc
      721 ataatcaaac caggcacgat gaattccata tatgaaaaac cacccatgtc accgatccga
      781 ccaccgatca tttttccgaa aataacaaag tacaaggatg tcgtgatcac aggcggaacc
      841 agtgtttgga cccagatacg caaatagcgg ttcgtttcct tgatagctaa actttttaat
      901 gcagtaaaat aaagattaaa catttttctc ctccgtatga ttattttctt ctgtgatttt
      961 caaaaacagt tcttctaaac gattcgactt attcctcata gaaagaactt tgattccttg
     1021 tcttgtcagc tgatcgaaga ttccgttcac cccttgattt cgttcaactt cgacagcgat
     1081 tgtctgttca tcttcaaatg tacaagcgta tccttcaatc acaggtttgg tctcatatgg
     1141 cgctaaatca aagataaatg tttcaaattg cagtttggat agaagctctt tcatactagt
     1201 attctcgatc agttcacctg attggatgat cccgatgttg cgacaaagca tttcagcttc
     1261 ttctaaataa tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt ctcggagaaa
     1321 ctcccacatc tcgcgtctca attcaatatc gactcctgct gttggttcat ccaagatcag
     1381 caaacgtggt tcatgcatca atgccctagc aatcatcagt cttcgcttca ttcccccgga
     1441 aagcatacgt gcacggacat ttctcttttc ccaaagattg gactgcttca aatatttttc
     1501 gctgcgtttc atcgcttctt tgcgagagac accgtagtag cctgcttgat tcacgacgat
     1561 ttgctggacc gtttcaaagg gattgaaatt gaactcttgc gggactaatc caatctgttg
     1621 ctttgctcgg actagatccg tatccagatc ataaccgaag acattcactt ttccagatgt
     1681 tttattgact aaagaagtaa tgattccgat cgttgtagac ttcccagctc catttggtcc
     1741 taatagcgca tagaaatctc cttctttcac ggaaagatcg attcctcgca atgcttcgac
     1801 cccacttcca taaacctttt tcaaattatt tatttcaagc gcatttgcca tttatttcct
     1861 atctccttat agacatgatt ctttattgta tcatggaata cagcagatga aaaaacaact
     1921 tggttccagc gaaaaatcag gttgctgggc tcaggaaaat ctcctaatga aagacatcaa
     1981 tcggacgaag tcagacaacc ttttcagaaa aaacaaatcg aagataaagg attactattc
     2041 atatgtgcaa caaaaggagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa
     2101 acacgtataa acggcggaag caaaagccaa gatttttgat ccaataaata ctttcgatca
     2161 cttcgcctat cccaatacat gccactgccg cttttcatca aataacgcgg cttccgtttt
     2221 tgcggtttct actaaagtct ctgggtaatc caaaactttc agcaaggcaa tcgcatttcg
     2281 ggtccttgaa ggtccttctt ttaatttgaa atcaaaactg attccttttc cttctgttac
     2341 ttgttcttca aagtggacat tctcacaata attctttaaa atctctgtca attcgatatc
     2401 atgtgtggcg acaaatgcta aagatggata ctccgcaagc caccgaacga ctgaagcgga
     2461 agaagcaatc cgttcgatcg tatttgtccc ttttaaaatc tcatctacga agcagtagca
     2521 gcgttctttg gaagccactt ggtccaacaa acgtttgatc gatttgattt ctgaaacaaa
     2581 ataactgtcg ccttcaaata aatcatcttc gattgccatg gaagtcaaca catgtccagg
     2641 ttgcagggta aactgttctg caatcgctgt ttggatcgtc tgtgctagaa tacagctgat
     2701 ggctatactt ttgacatacg tagattttcc tgatgcattg gaacccgtga ctaatgtatt
     2761 cttctgccag ttgaccggat tcactaccgc ttcttttaac aatggatgat agatatcttt
     2821 cgctgtcaca ccgccctcag taaactctgg tcggcaagtg atgggcatat aagtacggta
     2881 attcaatatt gcagcggcta cttctaattc tcccagcaat tcccacagct tgatggcttc
     2941 agttgaatgc ttttctaatt tagtgagaac gaaatgatag gagataaatg gcagcatgaa
     3001 catgatgttg acatattcaa aaatcaactc tgcttcactc ccactcttca tacgaaatga
     3061 aaaacctaat ttagggatgt ctcttaatgg tttagtttgc tgtttcagct cttcttgaag
     3121 tggtgtcggc agttttgcta gctgattggc ggctgaaatc gttgaaacca aataactcat
     3181 gctattcagc tctgtttcta gtgtttgttt cttcactaga taatagaccg tattgaatac
     3241 taacccaccg attgccaaat agattcctag tacttgtcct aaaagaagca aacccaaacc
     3301 caccagcgga agcaagccaa gaaaaataaa taaacctacg cgtccaatct ttttactttt
     3361 tccatccgct agatattgtt ttgcaaaatt atgatctttt tttcctaaac gagcgaattg
     3421 atattggatt ttttctctca gttgaggatt ttcctgataa aaagcaatca gtttttctaa
     3481 ctgctgatct tcaccaaaat caaagctgcg taaacgttga tacaacgctt ctgaacctac
     3541 actagaatag gtactgttga ttccttcgaa tacttcgaac atatccaggt cataccaagt
     3601 aagatcatcg atttcgctat cccatgaacg gaacttcttc tccgtgagcc atgcttcctt
     3661 caagctttct tctttatcaa atctcggttg ataaggaatt ttttcccatt tgctacgaac
     3721 tgtttccttt aatttcaaac gtgaaaaaat gtctaaagca actaataaaa taacagctgc
     3781 aataataatt aaaacgataa attctctttg acccatatcc ctctccgttc ctttatttgt
     3841 tagctttagt atagcactgt tgcgctattt gaaaagaggg taaaaaaaca cctttcaaaa
     3901 taaaaggtgt ttttttcttg tatattacat atttatctct catttatcaa aattgacaaa
     3961 taatcatagg gaggaattgt tgttaattct tgtttattct ccagtgcatt gacaactgtc
     4021 ccaagggaaa ggatagcctc aggtactatt ttttcttgtt ctgttggatt agctaagcga
     4081 agaacaaatc cttgttcatg aaaataagaa ggataaatcg atgatacaat taagtcttca
     4141 ggtaattcaa tcaatgaaaa tttacgagga acatcaagtt cattttgtaa tggccaaatc
     4201 ttgttatcta acctgtgaat aaatacattc agtttctgct tttgataaga aatagattgt
     4261 tctaaccgtt tttctgcctg ttttgccact ttgaactcat taaatcttcc ttcttctaca
     4321 ttaaatccaa aagtgacttt atactcacct atctcttgag ctagcggtgt ttccatcatg
     4381 acatgccctt catttgtcgt atctcctgaa gctcgcccag gtcgccaagc aagattaggt
     4441 tttccaagtt cccctgttgt tgaaaataag gttatgatta attgattttc tatacgttca
     4501 tactctttta aaccatctgc ataaaacgta aaataatgat cattttctgc cacagagaca
     4561 ctcttatcga atatttctaa attaacaggt ttctctacca tagtttctga ccaattcgga
     4621 ggaatgacat caggggtatt ttcaataaat ccgttttgaa tttgtgcaat tgatttttca
     4681 gatttatcta aaacattaat tgctaagcgc atacgatgcg agtaaacagt attatgaatt
     4741 ttgagtgtgc cttcaacgat ttgactttct cccagactga acgatatttc ataagatatt
     4801 tcttccattt cgggtttttc agataaacga tcttttaaat ctttaggaag ttgagctttt
     4861 ccgtaaacaa ctaatgtttc ttgtaaagaa tctttatata ctttagctgt ttctaatttt
     4921 agagatagtt ctgtatctcc ttctaatggt gaataatcat acgtatcgcc atcatttgct
     4981 gaatcgatca aatgaacgaa atcaagatat tgtctatctc caactatcaa attcaattgc
     5041 ccatctttaa aagaaagttt atatttattg ttacttatct cagttttgtc tatctcgatt
     5101 gctgtttcta atttttcatt atttttctca aattcaacaa cagtgtatcc tagtgctggc
     5161 acatttacat ttatttttat gtcaagttca taataagctg gttcatcaaa atattcgaaa
     5221 cctgtagcaa tcatccgata agcatttttc cgttctggaa aataaacttc tttttcaatg
     5281 atattatagg gactaccttt gaagtgtatt ttctttgaat cagctactac atgcacgatt
     5341 ttcgatccac tataattctc agtcgttgtg ttgaaaacaa ggacttcatt cgattttagc
     5401 tttaatgcat cagccatccg ttttacaata aggttttcga ttccgtcagc tatctcatta
     5461 gcttctttga ttcgatggaa aatatcttcc gcaacattat ctgaaacaca gccaccaata
     5521 ctatcatgag cctggttttg caaaatcttt ttccataacc gacgaagtag gcctttactg
     5581 atatggatac catttctttc tgcaatcagc atgagcggct caactcgttt tagaattttt
     5641 tgttccaatt taaaattttc tattttcatt tttgttctta ctgagccaat agaacgatga
     5701 acacgagagt aaaccggctg acgaagttca cctttatatt caggtaattc ttttttgttt
     5761 ctgataactt ctacataaga aggataatct gaaataatat tgtggaattt actgtattta
     5821 ttcaacttct ctacgacttg atcaaaattt aaaatcattg acttttgatc tacacccgaa
     5881 ggtactaaaa tatctttgtt ttttccttgc atatgaagca tttcaactgc ttcatccaac
     5941 ttttcttttg caaattcagg aatatcttta attgcgtcta aggtcatcat tccagtcata
     6001 taaccaaatg gaaaattcat ggcataaaca aatttatctc ccaatccttt ccagataaaa
     6061 taaggagatg gaaccatcgt ttcaaagttt aatcctcgcc aacacatgaa attatcgatt
     6121 tctacttgat gcaaaagtgt aggcaaattc gcattgaacc caaacgtgtc aggcaaatac
     6181 cctaccatca ttggatctcc atatttaagg atcgtatcac tgattccaat cattaagttt
     6241 cttaaaattg attcagcatc cactaataag gcatctgttt gtgcatacca cggtcccacg
     6301 aatagttgtc ctgttttcat cagtcgttgg ataaccttaa gcttttgcgg attgatctcc
     6361 aaatactcat cgacgattga agtttgtcca tctaaacaaa agtttacttt agggttcctt
     6421 tccaattcat ctagaacttc agtaaagacc tgatcagaca atacgatggc atcttcattt
     6481 gtaaaatacc attctctgtc ccagtgcgtg tgttgaatta tatgtgcatt taccatatca
     6541 gattctccta ctttccttct tctactgaat ttacttgttg ttctttttta tacttgatgg
     6601 attttagtgc attgaccatt aacgctgtaa ccactccccc aataacaatt gatactaaat
     6661 ataaaggtat attcgtaaca aaaggaattg ccataaaggt tgaaagcatt gtttcttgag
     6721 taacaccaaa aaatccattt agaccacatg ctactgcagt gcccaccacg atgcttggta
     6781 ttactcgtaa aggatctttc acggcaaacg ggatcgtccc ttcagtaatc cccacaaggc
     6841 ccataatcat agctgtcttt gcattatctc gttccgattc atcataaagt tttttgttta
     6901 aaattgttgc taaaaacatt ccaaatggcg gaatagcaat tcctaataac atggctgtac
     6961 taggtccaaa gcttccgctt gaaaaagaag caaccccaaa agcatatgcg attttattga
     7021 taggtcctcc catatcaaac gccatcattc ctcctaaaat agccgacatc aaagcagcat
     7081 ttgctcctga catgccttct agccaattag tcagtcctgt tgtcattgct tgaataggcg
     7141 tagctagtac ataccataat atgattccaa caactaatgt agtaacaaaa ggaatcagca
     7201 taattgattt tagactaata acttgcatcg gtacttttac ttttttcaat gcattagcta
     7261 aatagccaca agctaatcca gcaaccattc ctccaataaa tcccatacca agattttgac
     7321 acatcatccc agtgataaaa gctggtgcta gaccgggacg gtcagcaatt gaatatgcga
     7381 tataggctga aatcatcggt acgataaact gtaatcccgt ctgaccaatc attcgcagga
     7441 ctccccagaa tgtatccgtc tggtcccaaa cacctgtgcc accaaaaatg actgcaatag
     7501 aagttagtaa ccctgctgct acgacaagcg ggatcatata ggaaacccca ctttggaaat
     7561 gatttagaat ttctcttcct tgtttcttga attccattag taactccccc acgatatatt
     7621 ttttatttgt taattaatgc aattgcttca tcaatcgtct cttcaccatc ttttactacc
     7681 tctgaggtcc ctactgttaa aatcggttta ccgtcaaagc gacctcgttc tgaaacattt
     7741 gtatctactg caaatatgac aacatctgcc tcggaaacat ccgaaattga aagtttgttt
     7801 tctatcccca tagccccctg tgtttctact ttaatatcta accctttctt cttcgcaaac
     7861 ttttctaaat tagcttttgc catataagta tgggcaatcc ctgcgataca tgctgttact
     7921 gctactattt ttgtcatatg aaattcctcc ttttaaaatg atagagcttt taccaattct
     7981 tttttatttc tcgaatcttt tattctttta acaaactcgt catccatcaa tcttccagat
     8041 aattctgcta aaatacgtaa atgattagta cctttatcag tgtcttttat tgctaacaaa
     8101 aagacgagat caactggttg atcatctaat gcgccccatt caacagaccg ctttaacttg
     8161 gctacagcca ctgttgattc aattactgct aggcttttgc catgaggaat ggcgatattt
     8221 ttcccaattc ctgtggtcat atggttttct ctgtcaaata cgtctttttg aaattgtttg
     8281 tggtcagaca caaacccatt agtctctaac aatttcgata atttttcgac tacacttttt
     8341 ttatcagatg catttaaatc tatcactata ttatttatat tgattttttc actgatcatt
     8401 tataagcctt ctttcaatat ataaactaat ttttcaaacg acgaactcgt ttttaaattt
     8461 gctaataact catgattcga agcgatttca ttgaatgttt cataaatttt attcaattca
     8521 ggttttactt tttcattaag tgcaaaaaag aaaacagaat gaacaagtgt cccatcccaa
     8581 tcaattcctt ctggattaac gtagacacag attactggct tttttataaa atctacttcg
     8641 atatgcggca tagcaaaatc attcatcgct gttgaagata gtttttctct tttgattgct
     8701 ccctctagta tcccttcttt cgcgtaattt tcttttacca attttttggt aattatattt
     8761 aaaacgttca cttgattatc ctttatttgg tgggtaaaaa agcaaaatcg tttatctaat
     8821 aaatttagaa aagcatcttc tgtttgttca tttaactgta gatactgttt tacttttctg
     8881 acatctgccc ctaacatcaa aggactaaca acaataacag gaaaatttgt attttcaatc
     8941 ggaatcgtgc ttataacaag ttcatcagtg acttttctat cttgtagttc attgatgctt
     9001 agtacatctt ctatctcaac catatctgca aaaccgttat ttattctttg ctctaataat
     9061 tttgatgttc catatccgct actacaaact aatatcactt tcgttttgtc aggtttgtaa
     9121 cgatccaaaa gtgattgaat atggagtgtt acgtaagcga tttcatcttc attaaaatgt
     9181 atacagaatc tctcttcgat ttcctctaat aaatctacac ttatcataaa tgacctttta
     9241 aaagaatatt tgattttgtc cgtataaggg ttatagatat ttactcccaa tttgagtctt
     9301 ttaactgctg cattgagatg taatactaga ttattgatca attcttcatc aggataaaat
     9361 tcatttaatt taattcttac taactcttgt agctcaacac ccatatctaa ataggaaagc
     9421 ttcgtttgat ctaagtcgtg attcatagca tttttttcaa tcgaagtaat gtgaatgtca
     9481 acataagctt gttcatctat tggcacaata attgaaaatg ctttttcaat cattttgatt
     9541 aaaataagtg tattctttga gatttcttct tcagaattat tactacgtga taagaaattg
     9601 cttgtttcaa tcctttctat cgcaatagca aggtggatag ctaaagtttg aaattcataa
     9661 tctgtaaatt tcaattgtgt cttattcatg tatcgattaa gcaattcggc tacttttttc
     9721 aatatgtcct tatttaagaa attattcgct tcaccaatga aattgatatt caagtctaag
     9781 agatctgttt tactgttaga tgctgtaaag ccaccccaat aatagttcac aacttctgac
     9841 atcaaccttc ttttactttt ttcatcagct ttgatgcgca cgccatttcg atctgaaact
     9901 aaggacatcc catgcttgtt tagtcttttt ctaacatctt tcattgtgct ttctaccgtc
     9961 gacctgctca cgtataattc gtcagcaaga ttttgaatct ttagagaatc attagtattc
    10021 aataatttag caaacacata agttagacgg tcctccggag agtctgcgat attgatttta
    10081 tgactggata ataaatttcg aagtgtttct ttttttccaa taagcgaaac gcctttttta
    10141 ggttgaacca acatttgtac atctggcatt ttttggatta tttctttcaa aattaaaata
    10201 tctttgctaa ttgttttcgt agatacatat aattgttctg ataattcttt gtaagttgtg
    10261 tgctcatttt caatcaaaaa gtatagtatt ttcatttgcc gttcgctaaa ttcgatgccc
    10321 attatcttca cctcctgaat acagcttaag tgtacaatta ttatctctat tttcatacag
    10381 aagaaagttc ttgattaatg tagaagtttc taatattctt gattatttca aatcaataaa
    10441 aaagcctgca atatttatat cacaggcttt tttctctatt aaatatatat aagataatac
    10501 atttttctac tagttctcac taatcttgta taattaaacc ctccgcaact tgacggagat
    10561 ggagaagatg cccccgtcta atctagcggt catctctcct cccattcctt ccaccaattt
    10621 ttttgacagg taaagcccga ttccgctaga ttccgtattt gtgcggctgg tattttcggt
    10681 ataaaaacgc tggaagactt tttcaacaga ggtttgcgtt cctggtttga taggattggc
    10741 cactgttaat cgaatgaatg gatcctcttt tgaaaatgtc acctttgctt gtccatctgc
    10801 ataacgcaat aaattctgta tgatattttg cagcactcgc agcaacatga gcggatctgc
    10861 taatacagtt gtatttatcg agtgttcagc gaacgctacc tcgatatttt tctcagaaaa
    10921 ttgttcataa aaagagaaaa atacttcttc cacctcttta gaaaaagaaa gcgtatggag
    10981 atccatttct ttttgcttcg tctctattcg agcaagatca taaaactgat ccgtcatact
    11041 gagtaatctt gcaacagatt gtttgatgat cgttaaatac ttctcccgtt tctccgtatc
    11101 ctctacctgt tccagtaatt gtacataacc gttaatagca gtcaaaggag ttctgagatc
    11161 atgagaaagg ccgacgatcg atagtctgat gttttcttcc atttcttgaa atgcttgtcc
    11221 tttttgttca ttttcatcca accatgtatt cagtgcatct actaggtcca ttagattctt
    11281 ttctcgaaat tcgactgaaa gacgaccacc atattcttgt cgttcagaaa gagtggcgat
    11341 tttcttagtt aatctcttga tttccctttg caggaagaaa ataaaacttc ccataaagat
    11401 aacaacgaga agcaacagcc atttaatcaa atctaaaccc cactccccac accgtatgaa
    11461 tatacacagc atgtgccttt gctagcttcc ctcgaagatt ggaaatatgc atattcactg
    11521 ttttctcact atcaaaatat ggttcttgcc aaactgtttc ataaagattt gctcgcgaaa
    11581 atactttttt cggatgttct aaaaaaagct tcagcagatc gaattctctt cctgtcaaat
    11641 ggacagccac accattcaca tttacatccc tcgactcttt gtccagtaag atttccttgt
    11701 aatagatgcg ttgcgacaga tcttttactt ggacagaagt ctgatgacga agttggatgc
    11761 cgattcgtaa caaaagttct tcgatatcaa atggtttcgt cacatagtca tccgctcctg
    11821 ctgccaacaa ggaaacttta tctctttgat cgctttttgc tgaaagaacg atgatcggaa
    11881 cattcgatgt ctgtcggatt tctgctaaca atgcttcacc tgaaagccct ggaagcatga
    11941 gatctagaag gatcaaatcg acaggatatg tgcttaacaa taattttcct tccgtacctg
    12001 aataagcatc aagaagtttg tatctctgtt caagaacctc ctccagtaag gaatgaacag
    12061 cttcatcgtc ttcgataatc aaaatagttt ccagcaggac cacctactta atgtcagatt
    12121 tttggaaaat aaacattcct agagctgatg ataccacgat tgttactaga ctgaccagaa
    12181 tcattttcgg tgcttgcgaa taagttgctt gttgcgcaaa gtccgtatac atgagccacg
    12241 tttccgtaaa ccactccgtt agaaattcca agcctttgat ttttgcgaaa acaccgggaa
    12301 tgaaggccgt tcccatgata taaagaaaag aaagaagcaa actgacactg gtacttctag
    12361 tgatgaatgt caaagccact ggaacagata atgtccctaa tgtcagcaga agcaatacag
    12421 gaaatacttt tagaacataa tttccatagt ttttgattcc ctcaacttca ccaaatccga
    12481 aagcaagtgt gtaagcaagc atggctagtc ccgtgatcaa aaccacgcca agtgttgtga
    12541 tcaaaagcgt aacgagaagt ttcgacacat aataagtcat tcgattccgt cctaaagata
    12601 acgtattgcg aatcgtaccg ttttgaaatt cttccccaat ataaaagcac gcaaatgctg
    12661 cgaaagccag aagaatccat gcagccattt tttctaccat taaaatccct gttgcaccgt
    12721 tgatgactgt atccgttgct tggtttaatt cattgccgta ttttgtatgc atataccatt
    12781 cgttccaaga ataaaaaagc ggaagtacga ccatcagtcc tagtgagacc cacattttac
    12841 ggctcttcca tacttttatt ttttcagcag ttaatagatt aaacatgtgg attgcctcca
    12901 gttaattgta agaaataatc ttccagtttt tgcccaacta ggtgaatacc atccacagga
    12961 acttttgctt ctaccaatgc ttgattgatg gctgctactt ggtcaattcc ttcaaaaagc
    13021 tggataacac ggctagattg tacaaaataa tccctataac caagagagtc gagaaccgtt
    13081 actgcttgtt ccgtcgccga agtgtcaatt tttataaatt gacgagattc ttgtgccaat
    13141 tcttctttcg ataattcttt gatcaagtgt ccttcatgca aaataccata gtgagtggcg
    13201 atctgtgaca attcatctag caagtggcta gaaagcaaga cggtgatgcc ccgttctttt
    13261 accaaacgtt ggatgatttc tcgcatttct gccatccctg ctggatccat cccattcgtc
    13321 ggttcatcca aaatcaaaaa ttctggattc gtgatcagtg catttgcaat agctaaacgc
    13381 tggcgcatac ctaaagagaa attctttgct ttctttttcc ctgtattttc caatcgcatc
    13441 attttcagca ggtcttcgat ttctctatcc gacacaccgc cgttcgctgc ttggatacgt
    13501 aggttatctc ttgcagttaa ttctggataa agagcagggg tttcaatcga ttgacccata
    13561 cgtcgtcttg ctgcctgcaa atccgtcgtt ccaaataatt cgatcgaacc ttcatcaatc
    13621 gagatcaaac ccataatagt tctcataaag gtagacttac ccgctccatt ttccccaatc
    13681 aatccataga tcattccacg tttgatttcg attgatactt tatctaatgc tttggcagca
    13741 ccgtattttt tggttatacc agttgctttc aagaccgttt catttccaaa atccatactt
    13801 atccttcttt cttattgatg agtgaagtat agttgacggg tataaagaaa gcagccagaa
    13861 atgtataaag aaagtgtaaa ttttccaatt gaatgtcaaa aaaaacaatc ggacaatacg
    13921 tgtctttcct attatcaggc agtttgttat acgcttgtat cagtttcctg attctttctt
    13981 ttcgaccaaa gtcgtttctc tgtattcctc ttcactgaaa atttttgctt ctttcagatc
    14041 aagcttgagt aattttacca gaccggttat aaggataact atagcaagac tccctcccag
    14101 aaaaattaga ctgtctcgca gacgtggttg cgttgtcttc caatcaaaga gcgcaccaaa
    14161 cagtgctaat ccaagtggac tggccaccat ggtaatcgtt gttatcgttg acataagtcg
    14221 tccttgtata tgcgttggaa cttctcgctg aagccagacc atataaaaag caaaagaaaa
    14281 cattaatcct taaaaacgga cttatgaaaa ataatacatg ccgtttggga tttacaatta
    14341 gctaccaaat aagaggttat gacaaaagac ttttcacaat cccttcttcc gaataaacgg
    14401 tgttctcctg ccaactcctc ggtcacaagt cacaatattt atcaaacatg agaagcaatt
    14461 tttgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct cagaagcgga
    14521 taaataatat tgtttgatga tactttatct ttttcccgtt tgtttataga tcgaatgata
    14581 ttatagacca agaatcctac ctattaacat agctaaaaac acaaaaaagg cactttccaa
    14641 attgtggaaa gtgccagaaa agttaggatt aaggtaactg cgctcagcat tgcttcgccc
    14701 agtacgattc tcgtaactcg tgaactcgaa cgataatcgc aattaacgtt ttgagaactg
    14761 tgaagcttta cgggctttct taagacctgg ttttttcttc gtatcattga ataagtttga
    14821 aaaaccttga tttataaagg ttttcccgta aatatatttt attatatttg attaaatttg
    14881 aaatgtttgt gttctaaaat gtgttctaat ttaaaggcag tatatttcgt attttagatt
    14941 aaatttttag gctcattcat tctcaaatcc attctattca gtcaataaaa attttaaaaa
    15001 gactttataa acaaagatgt tcaagcgagc atcttatttt tttgtgctta atttgaaagg
    15061 agaacatttc atgtataaaa aacaaaaaaa gaaattatgt gtattaacta ttttgagttt
    15121 ggctgttcaa gcaattgttg gagcaattgc tcctacgatt acttttgctg atgaaattac
    15181 ccatccacaa acggtaaccg tagaactaga tttagcacat caatatgctg ttgaaggaac
    15241 ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg cattatgcag tgtacaacgg
    15301 ctttaaacaa gatattttct gtattgaacc aggagtcccg atttacaatg agttcactcc
    15361 tggctatgag aaaaacccgt taccagatat gtccgataaa gccaaattag tttccgtttt
    15421 atggaaaaaa gtaggtacag atgcagatac tcatattgtg gcacagaaga tgatctggca
    15481 agaagttaat gggtataccc ttcactcaat aaaacgatca gatggtagtg ccgtaaatat
    15541 cgcagccatt gaggcaaaaa taaatcaagc cattgacgat tatcagaaga aaccaacctt
    15601 ccataatagt acggctaaaa cagtattagg gcaatcgact actgtgacgg atacgaataa
    15661 tctgaatttg tcagagttcg atgaggttgt ggaaaatgcc gcaaacattg attatcgtgt
    15721 gaatggtaat cagttgataa tcaccccaaa tgccaattca aaagaaagtg gtgtgctgac
    15781 gcttaagaaa tctgctggta ccggaacacc tgtcgcctat aaaaaggctg gccaacagac
    15841 tttaatggcc ggggcgattg ataaacctaa tacctacaca atcaaaattg atgtggaaac
    15901 ggagggttct ttaaagatca aaaaagtcga taaagaatcg ggtgatgttg taccaggaac
    15961 ggttttccat ttagactttg gaaaaacttt acctgcaaaa gacgtgacta ctgacaaaga
    16021 aggcattgct acattggatg agattcctca tggtacaaaa gtaaccatta ccgaaaaatc
    16081 agtaccggcg ccttatacga ttgataccac ccccatgact accaccatta aagcaggcga
    16141 aaccatttac gtgacttcaa aaaacgcacg ggaaaaaggg caaatcattc tggataaaag
    16201 cggagttgaa acagggagtg atctttggaa tgacaattat tccttagctg gtaatacgtt
    16261 tgccattcgc aaagacagtc caaccggtga aatcgttcaa gaaatgacca cggatgaaaa
    16321 cggtcatgcg gaaacaccaa aagagattgc taatgcgttg gaattgggaa cctattatgt
    16381 gaccgaaact aaagctagtc atgggttcgt gaatacgttc aaacctgtaa aagtcgaatt
    16441 gaagtatgcc aatcaaaccg tagcccctgt taccagtaac gtaaaagggc aaaatcaaga
    16501 agtcactggc gaaaccactt tgacgaaaga agacaaagat actggggata aagcacaagg
    16561 taaggcagtc tttgagggtg ctgaatacac cttgtttact gctaaggatg gaaaagctgt
    16621 taaatggagt gaggctttta aaccagagtt agcgaaggga acgaaaggtt ctgatgaaac
    16681 ggtcactttg actttagacg aaaagaacca agttgccgtt aaacatttag ctatcaacga
    16741 gtattattgc caagaaacaa aagcgcccga aggatatagc cttgatgaaa caaagtatcc
    16801 tgtatccatc aaaaaggtgg atgatagcga aaaaaatgcc gtgattaccc gaaatgttac
    16861 ggcaaaagaa caagtgattc gctttggctt tgatttcttt aaatttgctg gatcagctgc
    16921 gggcactacc gaaactggct tcaatgatct gacctttaaa gtgtcgccat tggaagggac
    16981 caatgaaatc acaggtgccg aagatgaagc gaccacagct tataacgagc aattagggtt
    17041 tgatggttat ggcaagtttg aaaatcttcc ttatggggat tatttacttg aagaagtaga
    17101 agccccagaa ggttttcaaa agattacgcc attagaaatc cgttctactt ttaaggaaaa
    17161 caaagaagac tttgtgaaga gtgagtatgt ctttaccatt accgaacaag accaaaaaca
    17221 accaattaaa acggtgaccg ttccttacga gaagctgacg aacaaagcat tttctgttag
    17281 tttgaaccgt ttaatgctct atgatttgcc tgaggaagaa gatagtttaa cttctcttgc
    17341 gacttggaaa gacggaaaca aagaattaac ttctctggat tctaccgagc tagttgataa
    17401 attgagttac aatctacatg aaatcaaaga agactggtat gtggtagcgc aagccattga
    17461 tgtggatgcg acaaaagctg cccaagaaaa agatgaaaaa gccaaaccag tggtgattgc
    17521 agaaacaagc gcaacgttgg cgaacaaaga gaaaacagga acttggaaaa ttcagcataa
    17581 attaaccgcc gaacaagttt taaataaaac tattgtctta ttcaactatg tgtatgaaaa
    17641 taaggaagct tttgaagcag gcgataagcc agtagcgaag gatgttagct tgaataacca
    17701 agcccaaact gtcagttgta cagtggaaca ccatgtttct atccaaacaa aggcccacct
    17761 agaaaacggt tctcagacct ttactcatgg tgatgtggtg gatatgtttg atgatgtgtc
    17821 gattactcat gatgtactgg atggctcaaa agaagctttc gaaacaattc tgtatgcttt
    17881 attaccggat ggtacaaaca aagaaatttg gaaatctggc aagattgatt atgaagtaaa
    17941 tgataaagaa ttcaccaaaa ctgtgcttgc gaaaaaagta gataccggaa aatatccaga
    18001 aggaaccaag tttacttttg cggaaatcaa ttacgataaa gatgggaata tcaatggaaa
    18061 acacaatgaa gatttgaaag aaaaatctca aaccttaaca ccaaaagaag ttccaaccat
    18121 actaagtacg ccgaaacaac cggaaacacc aactgttcca agtggctctc aagaatctag
    18181 tcccacagtg aagaccttcc ctcaaaccgg ggagaaaaat tccaatgttt tactgttcat
    18241 tggttttact ttgatttttg cgacggcggg ctattatttc tggaatcgcc ggaactaagg
    18301 tgatgagatg atacaagtaa atcctaagaa ccaacgtgcg cgaccgcctc ctaaaaaagt
    18361 agtcgcgcta tttttgtatc caaaactaac aatgaaaaga ggaaaagaag atggaattga
    18421 aatttgtcgt gccagatatg gctgaaacct ttggaaagtt aagttatgcc ggtgaaggag
    18481 aaattataac agaaggatat ggacggaata ctacggtgat tggtcgcagt tatcatttgt
    18541 attccagtaa gcaacgggcc gatgatattg aggtggtagt agctgcggaa gccggtgaaa
    18601 aggattttga tcaagaccaa ccgctaaaag ccgtgaatcc ccatttggtt gccaaaggct
    18661 atgagattga gaatcgtggg tttactgatt acgtgttgta tgttgatgat ttagtgaaag
    18721 cataggagga aaagaagatg agattaacag aaggtattgt cgtagattca ggtttaacat
    18781 ttgggaagtt gcgtttttcc gcattacgcc gagaagtacg gaagcaaaat gaggatggaa
    18841 caattggtaa tgaggtaaaa gaacgaacct ataatttaaa atcctctgca caagggcgaa
    18901 tgattcaagt tagtatccca gctaatgtac ccttacgcga gtttgcttat gatgcagagg
    18961 tagagttggt caatccaatc gtggataccg ttgccaatta tgtttttcga gaaggaacta
    19021 ccgttaattg gttcattaag gcggatgatt tagtcttgaa gcggcagccg aatcaaggaa
    19081 atcctacgaa ccaaaatgaa agaaagaaat aggtgaagat tatggaaatt aatgttgaac
    19141 atattttaga ttgtctcgat caatatggaa aaggcgagct gactgaggaa caactcgtta
    19201 aagcattaac ctctgatgaa aaaatgtttc tgattataca tcaaggttta cttggagaaa
    19261 atagcgacag tgaagaagac tctgatgtac tagactggtt aggagaagaa gagtctttct
    19321 ttatagttgt cgaagtaaat gaaaacttat gtcggcaggc agaatccatt ttggaagaaa
    19381 ttggtgtgga gatgccggat gctattgaag gctttttaaa tcaattggtt gaaacgaaac
    19441 aactcccctc ctcgtagcag taaatgacta gcataacttt ttgaaattgc ctaatgaaat
    19501 cctattgggc tttttcgatt ggaggtggaa accatcaaaa tgtacaaagg acatcgcata
    19561 cgcgcaggag atcagcactt ggtttatcac tttgttcttg gctggctact cgctctgttc
    19621 atcggttgga tgagcgtctt ttattttcaa gagtttagac aatttgatat ttctaagcta
    19681 tcactttcta ctattgaaat cgtttggtct ataaaagatt tagtatgtct gcttggaagt
    19741 ctcgcttttt caggagctat gatattgctt tatattcact tttttcttga tcattggcga
    19801 agcttgtggc atcgacaaaa gctagctcgg atgattctag agaaccattg gtatgaagtg
    19861 aaacaaactc aaagtgaagg ctttttcaaa gatttgaata gtagccgaac cagagagact
    19921 atcagttact tccccaaaat ctgttatcga atgaaagatg gattactgtc tattcgcgtt
    19981 caaatttcac ttgggaaata ccaagatcag cttttaaaat tggaaaagaa gttagaaagc
    20041 ggactgtatt gtgagttagt agaaaaggaa ctcaaagact cctatgtgga gtatacttta
    20101 ctgtatgata tgatcgccaa tcgaattggg atagacgaag tagtggcaga aaatgggact
    20161 ctgcgattga tgaaaaatca agtatgggct tatgattctt taccccatat gttaatcgct
    20221 ggtggcacgg gtggagggaa gacctatttc ctcctcacca ttatcgaagc actattgaag
    20281 tcagatgcag aattgtttat cctcgatccc aaaaatgcag atttagctga tttaggtacg
    20341 gtgatgcctc atgtttattc acaaaaggaa gaaatttctg cttgtgtgga agatttctat
    20401 gaacgtatga tggctcgtag caaggcaatg aaagaaatgt ctaactacaa aacaggagag
    20461 aattatgcct atcttggact tccacccaac tttttaatct ttgatgaata cgtggcttat
    20521 atggagatgt taacgacgaa agaaagtgcg gtgattttga ataagctcaa acaaatcgtg
    20581 atgttgggtc gtcaatctgg tttctttctg attttggctt gccaaaggcc ggatgcgaaa
    20641 tatttaggag acggaatccg cgatcaattc aactttcgcg tggctttggg tcgtatgagt
    20701 gaactcggat attccatgat gtttggggag gtcgaaaaaa acttctttat gaagcacatc
    20761 aaaggtcgag gttatgtgga tacaggtggc agtgtgatta gtgagtttta tacgccactt
    20821 gtaccaaaag ggtatgattt cttagattca attagtaaat tagaaaagat gaatgattga
    20881 gtgatcttca atactgactt tatacttcaa gtaacgtata atacttttat atagtaggaa
    20941 gaaaggggat aattaatatg gaaagtaatg attcttattt agagtatgca aaagcaaata
    21001 aggataagtt tatttcttat attatcaaag ggaaatcccc cgaagttgga cgagacgcag
    21061 tttttatggc agggagtcct ggggctggaa aatcagaggt tgccattgga ttagctgaga
    21121 attatgataa ccatgtgatt attgatgcgg attactttcg cacccagttt cctgattata
    21181 atggaaaaaa ctctagtctc tttcaaaaag catcttcttg gttggtagag caggcattta
    21241 gatatgtctt agatcatggc tattctttta ttctggatgc aacttttgca atattaagtg
    21301 ctgaaaaaaa tatctcgcgg gcgttgaaaa aacattttcg agtgacgatt ttctatgttt
    21361 atcaagaccc taaaatcgct tggaaattta cacaagaacg tgaaattgca gagggacgtc
    21421 atgtgccgaa aaaggcattt attaacgctt ttttcaaatc aagagagaac attgcaaaag
    21481 taaagagtaa acatccagat gtattgcttc atatcataat aaaagattat acgaataata
    21541 tttccgaagt tcattatgct acggataatc tagaattatt tttaccacta gggtatagta
    21601 ggaaagattt ggaggttcaa ttacatgact gagaaagaac aagtaacgaa aattgtaaag
    21661 aaatacaata agagcattgc ggatttatcg gaaaatgcta ctgcaaaaga gttcaaaacg
    21721 gtgattaaat atgtagccga tcaggccaat gaaaaacaac ggaaattagt tggattaaat
    21781 aaaaaatagt aggacaaatt a
//
LOCUS       DACEDO010000050        20496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEDO010000050 DACEDO010000000
VERSION     DACEDO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.050"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(13..996)
                     /locus_tag="IWO52_001921"
     CDS             complement(13..996)
                     /locus_tag="IWO52_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0356187.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1001..1648)
                     /locus_tag="IWO52_001922"
     CDS             complement(1001..1648)
                     /locus_tag="IWO52_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0356188.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1733..2365)
                     /locus_tag="IWO52_001923"
     CDS             complement(1733..2365)
                     /locus_tag="IWO52_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0356189.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IWO52_001924"
     CDS             complement(2381..2932)
                     /gene="coaC"
                     /locus_tag="IWO52_001924"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0356190.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(2929..3666)
                     /locus_tag="IWO52_001925"
     CDS             complement(2929..3666)
                     /locus_tag="IWO52_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0356191.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3663..4652)
                     /locus_tag="IWO52_001926"
     CDS             complement(3663..4652)
                     /locus_tag="IWO52_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0356192.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4910..5452
                     /locus_tag="IWO52_001927"
     CDS             4910..5452
                     /locus_tag="IWO52_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0356193.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5469..6554
                     /locus_tag="IWO52_001928"
     CDS             5469..6554
                     /locus_tag="IWO52_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356194.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6566..7372
                     /locus_tag="IWO52_001929"
     CDS             6566..7372
                     /locus_tag="IWO52_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0356195.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7373..8200
                     /locus_tag="IWO52_001930"
     CDS             7373..8200
                     /locus_tag="IWO52_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0356196.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8197..9270
                     /locus_tag="IWO52_001931"
     CDS             8197..9270
                     /locus_tag="IWO52_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0356197.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9698..12394
                     /locus_tag="IWO52_001932"
     CDS             9698..12394
                     /locus_tag="IWO52_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0356198.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12471..12959
                     /locus_tag="IWO52_001933"
     CDS             12471..12959
                     /locus_tag="IWO52_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0356199.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            12971..13465
                     /locus_tag="IWO52_001934"
     CDS             12971..13465
                     /locus_tag="IWO52_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0356200.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13486..14295
                     /locus_tag="IWO52_001935"
     CDS             13486..14295
                     /locus_tag="IWO52_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0356201.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14292..15113
                     /locus_tag="IWO52_001936"
     CDS             14292..15113
                     /locus_tag="IWO52_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0356202.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15164..17263
                     /locus_tag="IWO52_001937"
     CDS             15164..17263
                     /locus_tag="IWO52_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0356203.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17260..18378
                     /locus_tag="IWO52_001938"
     CDS             17260..18378
                     /locus_tag="IWO52_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0356204.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18417..19295
                     /locus_tag="IWO52_001939"
     CDS             18417..19295
                     /locus_tag="IWO52_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0356205.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19486..19638
                     /locus_tag="IWO52_001940"
     CDS             19486..19638
                     /locus_tag="IWO52_001940"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356206.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         6279 a         3526 c         4361 g         6330 t
ORIGIN      
        1 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
       61 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
      121 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
      181 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
      241 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
      301 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
      361 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
      421 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
      481 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
      541 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
      601 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
      661 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
      721 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
      781 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
      841 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
      901 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
      961 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     1021 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     1081 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     1141 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     1201 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     1261 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     1321 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     1381 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     1441 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     1501 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     1561 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     1621 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     1681 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     1741 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     1801 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     1861 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     1921 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     1981 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     2041 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     2101 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     2161 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     2221 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     2281 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     2341 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     2401 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     2461 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     2521 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     2581 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     2641 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     2701 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     2761 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     2821 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     2881 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     2941 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     3001 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     3061 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     3121 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     3181 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     3241 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     3301 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     3361 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     3421 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     3481 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     3541 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     3601 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     3661 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     3721 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     3781 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     3841 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     3901 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     3961 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     4021 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     4081 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
     4141 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
     4201 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
     4261 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
     4321 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
     4381 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
     4441 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
     4501 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
     4561 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
     4621 tttattacct gaaaagatcg aaaagcgttc atttgtgaga cactcctttt aaaattttct
     4681 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
     4741 taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa gaaatattca
     4801 gcaaaaaaaa ttttacaaat ggttgccttt ttcaataaat ttcgtatact aatacgagtg
     4861 gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta tggaaattgg
     4921 tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac taggcgaacg
     4981 cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt caccatcaat
     5041 ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat tttttcatca
     5101 agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt atttcgatga
     5161 agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag aaatggagcc
     5221 tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt cattatctga
     5281 aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga cggtctatgt
     5341 ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat taaggaatca
     5401 tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt aatatagggg
     5461 gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc tatgatgatg
     5521 aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat acactgcttg
     5581 gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc accgaagcta
     5641 cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct aataagcgac
     5701 aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt tttgataacg
     5761 ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag
     5821 atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt gaaatgtctg
     5881 gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg aaagtattgc
     5941 tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg cagtatgaac
     6001 tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat gaccaagaag
     6061 aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc gtccaaagtg
     6121 gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat tttgttgggg
     6181 agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt gttggtaagc
     6241 aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt gttttacgcc
     6301 cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg gtcgatactc
     6361 agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa aatgaatgga
     6421 tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc tttgagccag
     6481 aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca
     6541 gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt ccgtccctta
     6601 tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt atcaatcttt
     6661 ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta cgtcggggac
     6721 atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt ttttcacgct
     6781 gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc aattatggct
     6841 gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg catttattgg
     6901 gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga tcgggccgaa
     6961 acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg agattccatt
     7021 tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga tcagtgctag
     7081 tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc cgttatcatt
     7141 aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt tattcatgct
     7201 gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg aacagcattt
     7261 cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa tcattgctat
     7321 gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa
     7381 atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg caccgatctt
     7441 ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta caggatttac
     7501 ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg tattgaatac
     7561 gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat ttggggctat
     7621 gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca tgaataacat
     7681 cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct ttacactact
     7741 aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca gcattccaat
     7801 cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg tcgatgcggc
     7861 tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc cttatctttc
     7921 tccaggaatc atagctggat attttatggc atttacctat tctcttgatg attttgcggt
     7981 tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct attctcgtgc
     8041 aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct tgttctccat
     8101 gatattggtc attggatact actttattag taaagaaaac cgtccaaaac ggctgaagaa
     8161 aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca atctctgttt
     8221 ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca
     8281 agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta tatcgatcct
     8341 agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga aacatttgat
     8401 tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga catcgctata
     8461 ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc attagatcat
     8521 tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga ttttgatcca
     8581 gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata taacgataaa
     8641 ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga attaaaagac
     8701 agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa tagtttaggc
     8761 tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa attgaatcat
     8821 ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat gatcaatgaa
     8881 gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg ggaaaatgaa
     8941 catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa tatcgttatg
     9001 ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat gctagaaccg
     9061 gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga agaagcaaaa
     9121 gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga tgacacgatc
     9181 tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta taatgaccta
     9241 ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat ggggacttat
     9301 caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa tcttctacca
     9361 aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac gtttttctac
     9421 cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag taaatctaag
     9481 attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac ttaacccttc
     9541 tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc tatgaaaaaa
     9601 cataaaaatc atattagata aaaactaaat tggtagataa attattttgt cagatatctg
     9661 aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat atagaataga
     9721 catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg aaaaaatgaa
     9781 aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga atatgtcttc
     9841 tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg ccagccatta
     9901 tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc ctgttttgtg
     9961 gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg aagcctttca
    10021 ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta tagccgctgt
    10081 caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg ttggaaaaag
    10141 ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg aaaaagatgc
    10201 accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc tttcggcaac
    10261 actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg atggtttatt
    10321 ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat cgaaagaaaa
    10381 tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg gtgaaaatgt
    10441 aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag gtgaaaatta
    10501 tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa cttttgaaga
    10561 tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg aagcggatgt
    10621 tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat ctcatccctt
    10681 tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg atgtgtatag
    10741 tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc agttaaatat
    10801 gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga aaaatcaaaa
    10861 gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa aaattactca
    10921 gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg aagtacagca
    10981 tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat tgatgattca
    11041 gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga cgacgagtgg
    11101 acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata agacagactt
    11161 aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact tatgtaatca
    11221 gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa gactgcttat
    11281 tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc tgttgcttgc
    11341 tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat gcggcaatta
    11401 tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg tcgaatcgac
    11461 aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg tagatatggg
    11521 ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt tacttcttct
    11581 gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga tgaatgcgcc
    11641 atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc aatactttga
    11701 aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg ggatttcgga
    11761 caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag ttttcacgaa
    11821 ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt ttgacaagac
    11881 aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta atattaatgt
    11941 ttatgacatc ttagacggtg atggagcagc caatttggag catattttga gtcctgaaat
    12001 gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt cgttggaagg
    12061 aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag tggaaacagt
    12121 catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa aactaaatct
    12181 ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag aaaaatctga
    12241 agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt ctagaagtat
    12301 ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc tttcgctgct
    12361 ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg tctaatctgt
    12421 tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca atgtttttaa
    12481 aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt aaccatatga
    12541 agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc agtattcaat
    12601 tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat gagtgtgatt
    12661 ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag atttttgttt
    12721 ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt ttggagaaag
    12781 agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa atagtattgg
    12841 caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc caacaggaat
    12901 taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat tttttttagg
    12961 aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg gacaagtagc
    13021 gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg atgaggcggc
    13081 taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg tcaaagtcat
    13141 tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga aggacatgcg
    13201 cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag ctttcccaac
    13261 ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat cggaagataa
    13321 gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact taaaagaatt
    13381 agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa aacgttcagc
    13441 tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta tgatgcattt
    13501 gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg gatgtccatg
    13561 ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga tttgcagacg
    13621 gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa tatcggaatc
    13681 tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc aatcaaaatg
    13741 ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt aggattacag
    13801 ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt tttcaaattt
    13861 gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg gtttgtccac
    13921 tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga tgttgtcaaa
    13981 agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc tggaaatctg
    14041 ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa tattgctgtg
    14101 tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat tgcaattgcc
    14161 gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact aaaaaatata
    14221 tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca agaagaggag
    14281 gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa ttggttcgga
    14341 agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg atgggcggcg
    14401 gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt gaagaagatc
    14461 gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct tgcgcgtcct
    14521 ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct ggttttgacg
    14581 tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga attggcgatt
    14641 cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac ttagccaatg
    14701 ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct aattacttga
    14761 tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt gaaaagctgt
    14821 atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt ctgatcatgg
    14881 taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt tcaatgaagg
    14941 gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga attgtaccgt
    15001 tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc atcaaaatta
    15061 tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca tagtaggaag
    15121 ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta ttaatgaagt
    15181 aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa atgtgactac
    15241 ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga ccttcgatgt
    15301 aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa cgttgacctt
    15361 ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata aacagctctc
    15421 tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc atttgtcaga
    15481 atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat atattcatca
    15541 ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta ataagcgttt
    15601 aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc ttcaagtttc
    15661 agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg tgttgatttc
    15721 tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg ccattgcttt
    15781 tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata tcgaaaaatg
    15841 gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa tgccaaacga
    15901 tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt tcggtatgaa
    15961 tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta atcgaatttg
    16021 gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg agttatgtca
    16081 aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa aatttacagg
    16141 aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta gtctttattt
    16201 tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac tgaatgcttc
    16261 aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc tttttccatc
    16321 tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca atctttgtct
    16381 ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac tgagtcttag
    16441 caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac aaatgactat
    16501 cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga aaacaaccta
    16561 ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt taagtgatgt
    16621 gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag acactacgtt
    16681 gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca ccatggcctt
    16741 ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca cttggggatt
    16801 agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc tgcacaaccc
    16861 tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag acggttcgtg
    16921 gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag actttggctg
    16981 cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc aatatttcgg
    17041 attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa cgttttcttg
    17101 gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat ccgagttgag
    17161 tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa ttcttcacgt
    17221 agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact aaaaaatatt
    17281 acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac aggcgagaaa
    17341 ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg tggcgcaatt
    17401 ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg aataattcgt
    17461 catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt acgtgattta
    17521 atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc tttaggggat
    17581 ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat agtggttaat
    17641 cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc taaaattacg
    17701 atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa ttacgctttg
    17761 gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg ggaacgcatt
    17821 accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg ctattacgtg
    17881 ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg cgagtttaaa
    17941 ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc agaagattat
    18001 tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca tgcgaaaaac
    18061 tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac aagagatcgt
    18121 tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat gccacgacat
    18181 ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt atcggtcact
    18241 ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga tgttgctagt
    18301 gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt cactgatcga
    18361 gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa cctatgatga
    18421 tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt actgaggaat
    18481 taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca atgaagcagg
    18541 tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc ggctgctttg
    18601 gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca acaccaaaac
    18661 ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat attccgattg
    18721 aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt tctggcaaag
    18781 ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt gccttgttca
    18841 atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg atcaagcgac
    18901 atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta gaggggatgg
    18961 cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta ttggctgacg
    19021 attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat acaacactga
    19081 tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa cacatgaaga
    19141 aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata ccaccaattg
    19201 atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc ggattagcat
    19261 tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca ttgatactca
    19321 tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa agcgaaaatg
    19381 aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca ctgaaaggaa
    19441 atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag gatattcact
    19501 ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt aatagttata
    19561 gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct tggcttaatg
    19621 tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta attctattat
    19681 agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta tacattttaa
    19741 atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttgc ctttaactct
    19801 cgcttctctt gcaaatgtag ttagcatttt aatcgataat aaactcaggt agttatatta
    19861 ccttgaaatg attgataatt ttgtcacttc tatttttttt gcaataaatt ttttatttta
    19921 gtgaaaaata agaaactatt tctttgaagg cgataagtta gaaaaatatt attgtagtct
    19981 tttctttatt aggaatcatc actgttgtat cttattacct tttctgtttt tcttattaga
    20041 gaaaaataat ttgagaggta gatagttttt attggaggaa gtaattatgt atatttttga
    20101 aacaaaaatt tttcattgca ataattgcaa caaagaaatt tcatcggggg aagaggtttg
    20161 gacaaaaatg gaaatttccg ccaaaagtaa ctgctcctca actgaaatca attaaagaac
    20221 tagaatttga gaatgcactg gtaatttgta tgacatgtac tgatgaagta tttcgaaaac
    20281 atttttaaag gttgagatgt atatagattt aaccaatcat ttatctctct cggaaaagga
    20341 atattaacta accagcgggt tatcaaaaat aggtaggaaa actgtactta atggcttatt
    20401 tcgcgctgat tatgagaata tcagtctgtt tgattaattt ttagggttca aaatacagag
    20461 acaaactcca ggtcgggttt cgccacaagc gaatta
//
LOCUS       DACEDO010000051        20113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEDO010000051 DACEDO010000000
VERSION     DACEDO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.051"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            595..825
                     /locus_tag="IWO52_001941"
     CDS             595..825
                     /locus_tag="IWO52_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356207.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            1010..1555
                     /locus_tag="IWO52_001942"
     CDS             1010..1555
                     /locus_tag="IWO52_001942"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0356208.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1556..1873
                     /locus_tag="IWO52_001943"
     CDS             1556..1873
                     /locus_tag="IWO52_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0356209.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(2030..2194)
                     /locus_tag="IWO52_001944"
     CDS             complement(2030..2194)
                     /locus_tag="IWO52_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HAQ0356210.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2296..2817
                     /locus_tag="IWO52_001945"
     CDS             2296..2817
                     /locus_tag="IWO52_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ0356211.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2789..3244
                     /locus_tag="IWO52_001946"
                     /pseudo
     CDS             2789..3244
                     /locus_tag="IWO52_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3284..3442)
                     /locus_tag="IWO52_001947"
     CDS             complement(3284..3442)
                     /locus_tag="IWO52_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HAQ0356212.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3535..4296
                     /locus_tag="IWO52_001948"
     CDS             3535..4296
                     /locus_tag="IWO52_001948"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0356213.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4483..5835
                     /locus_tag="IWO52_001949"
                     /pseudo
     CDS             4483..5835
                     /locus_tag="IWO52_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5851..7071
                     /locus_tag="IWO52_001950"
     CDS             5851..7071
                     /locus_tag="IWO52_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0356214.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7106..7783
                     /locus_tag="IWO52_001951"
     CDS             7106..7783
                     /locus_tag="IWO52_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0356215.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7780..8973
                     /locus_tag="IWO52_001952"
     CDS             7780..8973
                     /locus_tag="IWO52_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0356216.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8988..9314
                     /locus_tag="IWO52_001953"
     CDS             8988..9314
                     /locus_tag="IWO52_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0356217.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9318..9653
                     /locus_tag="IWO52_001954"
     CDS             9318..9653
                     /locus_tag="IWO52_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0356218.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9650..10045
                     /locus_tag="IWO52_001955"
     CDS             9650..10045
                     /locus_tag="IWO52_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356219.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10042..10413
                     /locus_tag="IWO52_001956"
     CDS             10042..10413
                     /locus_tag="IWO52_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356220.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10429..10989
                     /locus_tag="IWO52_001957"
     CDS             10429..10989
                     /locus_tag="IWO52_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0356221.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11114..11425
                     /locus_tag="IWO52_001958"
     CDS             11114..11425
                     /locus_tag="IWO52_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356222.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11449..11640
                     /locus_tag="IWO52_001959"
     CDS             11449..11640
                     /locus_tag="IWO52_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356223.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11640..15496
                     /locus_tag="IWO52_001960"
                     /pseudo
     CDS             11640..15496
                     /locus_tag="IWO52_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            15497..16369
                     /locus_tag="IWO52_001961"
     CDS             15497..16369
                     /locus_tag="IWO52_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356224.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16366..17415
                     /locus_tag="IWO52_001962"
     CDS             16366..17415
                     /locus_tag="IWO52_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356225.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17419..18243
                     /locus_tag="IWO52_001963"
     CDS             17419..18243
                     /locus_tag="IWO52_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356226.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18801..20033
                     /locus_tag="IWO52_001964"
                     /pseudo
     CDS             18801..20033
                     /locus_tag="IWO52_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6950 a         3436 c         4486 g         5241 t
ORIGIN      
        1 aactccttct aaattgttct tattatctat catccgttgc tgtctattgt ttttttaatt
       61 attcacacga taactaaagg tgagcgaaga gaaatgattc cattaataat ttcaattttt
      121 gcgctttgtc ttaatgtcta tatgattgga tttaaaaatg ggcaaaataa aagatagtag
      181 taactaagaa agaaattttt atattgtcac tgtggcggaa agggtagacg ctaagcatgt
      241 gtgctaggtc aatgcttcgg caactatgca atgttcgatt cattgccagt gactttggca
      301 accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat
      361 cgtgtaggtt gctattacat attagatcac tcgttgagtg gtctttttat tttacataaa
      421 ggaggtaaca acgatgtata gaccacaata cttagaacag aagtatgaag taatcactgt
      481 gcaaaacggt aacggtgaga tagtacgaaa gtatagaaga ccaataaaga gcgatacata
      541 taaacgaaag gaaaacaatg aagttatttc ttttcgcaga aggagaaaag ccaaatgaga
      601 aactactggt atgtatcgct aactaatgaa tatcctcgaa ccattgatga ttgttcagtg
      661 cgtgttgtgc gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat gctaagagaa
      721 gctacaccaa acgaagtgga taaatgcaag ctgatatatt gcggtcatgg ctattggaaa
      781 gacgagtata tccaacaaaa cattgagagg tggatagata gatgagttac ctcgaacatt
      841 tgaaacgttg ctacatgcat tctaagaata aacttcctga cagctacact gtagatgatg
      901 ttgctataca tgtattgaag actgagagcc atagcagtcc tgatggtagc agtaaggagc
      961 agacgctcgc gtggttcaag ttctttaagt ggattaagga ggaagagtaa tgcacatgca
     1021 cataggagaa aagatatata aattgagacg gcttaataac gaaacaatga acgacttagt
     1081 taaacaaact ggagtgtcaa aatcatggtt atctgatatt gagtctggaa aaaagcaacg
     1141 cgttgatttt aataagatat gtgcaatagc caaacattat aaagttagct tagaatactt
     1201 tcgggatgat tatttggata gtgatgaaca cgaggatatt attcctttga caggcgaaac
     1261 aagagttaaa tcagtgaaat cgcttgaaca aagcctaaag tatctgaata acaaacaaga
     1321 acctaacctt ctagagatca aactaaaaga tactgactca gtaccagagg tttggtacaa
     1381 gggtgagagg ttggatgaat tgcctaaagg attagtagat gtctcgtatc attggaagac
     1441 tgatgatttt actaatgatg atagaggagc gaacgacatc acgattcaat acttttctag
     1501 ctttaatgat aagtatccag ataagaaaac aatcggacac aagagagata tgtaaatgaa
     1561 agaagttaga cctagagacg aaatagacaa actatacaag accaaacgat ggcgagacct
     1621 aaggcaagta gtaatagcta gggacttcgg catgtgccaa gagtgcaagc gtcgagggcg
     1681 gaacacaagg ggcacgatca tccatcacat agtcgaggcg agggaagacc tgtcactgtt
     1741 ctggtccgta gataaccttg aatgtatctg tgtagcttgt cacaacagag agcatccaga
     1801 gagatcaggc gggaagaaga aaccaaaacc taaatcacat atcgttaaaa tgtattcaac
     1861 tcctgaaaga taagtttgca gcgaaatgaa ggtagccccc ctactctaaa agattaaaga
     1921 gtaaggcttg agaagaacgg tgctgtcctt ccttcgtaaa aagaccgctt ttcaagtttt
     1981 ttggagaaaa aggaaaaagc cgaccaattt aagccggctt tggacgaagc tatttcttag
     2041 tccatttgtt tcctttttga gaagtagggg gtaatcggtc gcctggatca atttttactt
     2101 cttgtccgcc ttggacattt ccaccacgag gacccacttc tttgtaggtt ccttttggtt
     2161 tgttgtcttc gccgggttta tagagttctc ccataggaat ccctccttaa caaatttcgg
     2221 cacagcactg ccgataacct aattataagg attgtgataa cgatttcaat ctatcttttg
     2281 aaagaaggtg atattatgcc gcaaccagcg aagagtgcaa aattacaatt attaaacgga
     2341 aacccaaata agaagaatac cgaagaactc cgcaagcgag cggccgcaga agacaaatta
     2401 aaaatggcta ctgacaaaat caaaccgccg ttatggctag attcgctagg aaaggatacc
     2461 tttgagttta tcgccgatga attgctgtct gtggatttaa tcagtaatcc ggacgtccat
     2521 acaatggctc tatactccaa ttggtattcg caatacgttt ctttagaaaa acagcttcga
     2581 aaactacaac gagagtacaa gttgaactat gcgcttgcga aaaaggaggc agaggcgaga
     2641 ggtgagccgt ttaatgaacc taatgaatta attggtaatc cgctctctcg gcagatggat
     2701 acagcgtctc ggaatctccg ttcttttggc gctgatttag gactatcacc agcagccaga
     2761 gctaagttag ctattaagat ggctgatgat ggtggtgatg acgatgacga cttctaatat
     2821 tttggatatg tcttacacag aacgtgtgga ctattggcaa agctatcttg aggagcaagc
     2881 ttcttggggt ggctttttaa aatgtccata tccggaattg ttaactactt ggtatgcgga
     2941 acgattaatc gatggaagca taccagccag caaagaaaat attcaagctg ctaaacggca
     3001 tatgcgtgat ttgcagcgcc aaggaacaga tgattttcct tggatctttg acgaagaaaa
     3061 aggtcaccgg cctattagat atatcgaaaa aaaatgtaaa ccaactgaag gcgactttgg
     3121 ttcgtttgtt ttgcaacctt ggcagcattt cataattgga tccatgtacg gatgggtaca
     3181 tcgtgataca ggagagcgtc gcttccgcga ggctcttatt tttgttggac gtaaaaacgg
     3241 taagttttgc cgtttaaaaa tcgggcaaaa tcggtgaaaa cctttatttt tggctttctt
     3301 ttagtaataa ttcgatagct ttatcaagca atttagacat aggaaccata gtttcttccg
     3361 acattttttt taatccttcg tataaatctt tatcaattgc gttagaaatt ctaattctgt
     3421 tttttagtcc gtatttgtcc atgtgtgaca ctccttttta ttttactata acatccgatt
     3481 ccaccgcttg caactaccat caattgatgg tagtatataa aagaggtgag tagattgaaa
     3541 aaatacatta tttacaaaca cttaaataaa ataaatggca aaatttatat cggtgtcaca
     3601 aatgatattg gcagaaggtg gaggagcggt gggattgaat ataaaccacc aaagaacgaa
     3661 acccaacacc acagaagttt ttggaatgca atacaaaaat acgggtggga aaattttgat
     3721 catttgatta ttgaagaaga cttaacgatg aaagaagctt ttgagaaaga aaagttttat
     3781 attgaacttt atgactcaac taataagaaa aaaggttata acatcgcaaa aggcggaaat
     3841 ggcggaataa tatataaagt tcaccccaaa ggaatgctag ggaaaaaaca atctaaagaa
     3901 ttttcttcaa atcacagtaa atgggcaaag aaccataaga ataattgtat gacaaacggt
     3961 gatgttgtat ggggtgttac tcacgaacac ccaaaaggca tgttgggaaa gcaccacagc
     4021 aaagaatcaa ttatgaaaaa gaaagcatat agtggtgaaa atgctgtaac ctcaaaacca
     4081 attgtcgcaa ttgaacggga tggaactaaa agagaatttc acagcgctaa gctttgcatg
     4141 gcttactatt cgattagtac atgtgttttt taccgacttt taaaagatgg cgccccttat
     4201 gtgataaatc ctaaagcgaa ctacagaaat aaagaaaaaa tattagctat cgaaggaatt
     4261 atgttcaaac ataaagaaga taccgaggta agttagtaga ttgcgaaagg ttactaacta
     4321 ccgtaacgcg taggagttga ataaatataa tgctcccaag agtgtccgac agcagataat
     4381 ttgctgaaaa tgtacgctaa actgggccag aactgactga ccgatgaaaa tgagggtgac
     4441 ctccagagtg cgagataaaa agctcgcaga taataacaat cgaaaacatc actaatttca
     4501 ggcctttcca catacatggt cgcttatgat gatgaacaag gcgccaacgt ttacgtattg
     4561 gcaaatgctc gtgatcaagc aagcttgttg tttgataagg ccgcagaaat ggtcaaacaa
     4621 tcgccggcgc tctttaagaa atttggtaag cctaaacgat caagtattaa ttatgctccc
     4681 gccttttcta aaatggaacc acgcgcctca gatagccgga aattggatgg gctaaacact
     4741 cactttggta tttttgacga gatccacgag tttacgaatt acaatctgat caacgttatc
     4801 aagaaatcaa gaggaaccag aaaacagcct ctgatagttt atatcacaac tgctggatat
     4861 gtattagatg gtccgttgat gtcttatttt gagcaaggtg tggactgttt ggaacatttg
     4921 gaagatgaca tcgatgaacg gactttctat tatctggcaa aacttgacag tgcggaagag
     4981 gctgatgacc caagattatg gatcaaagcc aatccgaata tttgtctaat gaattttgtt
     5041 ggcatgctag atgactatgt taaggataaa aaagatccaa aagaatatgc tgactggatt
     5101 accaagcaat ttaacttgtt ttccgatatc gatgagctgt catttgtcga tatgcctacc
     5161 attaaacgaa acaataaaac catcgatatt gaaacgctca aaggtaagaa gtgtgtcggt
     5221 ggttttgact tgtccgaaac ggaagacttt accgcagccg ttttagaatt tccgcttgaa
     5281 acaggcgagg tattcatttt gcaacacaca tggatcccac aagctagatt tgatcgagat
     5341 aacaatcaag agcgtatcaa agcgtgggag aaggtgggag atctaacgat tattcctggt
     5401 gattacgtca attatgaata cgtcttaaat tggtttgtag aaaattcgaa aatctatgac
     5461 attgtaaaaa tcaattatga caaggccaag gcgctacgat taaataaaga actagaaaat
     5521 gcaggatttg aaaccaacga gattcggcaa gggtttctat cattaggtgg gccaatgcaa
     5581 aacttcaagg aaatgctatt ggacggtaag gtgattttca acaattccaa gctttaccga
     5641 tggtatctat ccaacgtcaa gctggtgatg gatcgcaact caaactggat gccgtctaag
     5701 cagtccaaga gtagaaaaat agatggtttt gcagcaagtt tgaacagcca cgccgaagtg
     5761 ttgaatatgt tggttaatcc tgtcggaacc gggaaagtaa cctattactc gatttccgat
     5821 ttaatgaata tgtaagaaag gtgtggagga atgagtattt tagatcgttt gcgttctttt
     5881 ggccgagcga agccgaaagc gagcaaacaa gagtattttt tgaatgaccc gggattgata
     5941 ccgtatttag tcggaaaaga tgaaatatca gaagggattt tttccgtaat tagccgtgta
     6001 tcgaacgttt ttgcgtctct ccctctcaaa atgatagatg tggagtttgg ccaaccggac
     6061 gactgtcctg catacaactt gttgagcgaa ggccctcgat attttacaaa gtttgatttt
     6121 ttccgggacg tggaagtttt gagaaactac caagggaatg cgtatgtgca gattttccga
     6181 aatatcaatg gagaagtagc agatatggcg ttagtaaaac ctggtgcttg ccatccagtg
     6241 attgatatgg atagcgggga gctttactac caagtaactg cgactgacaa aggcagttac
     6301 aagcaagtta tctatgtaca ttacatggaa atgcttcact ttaaacaacc gaggtttggc
     6361 ggcttggaag gtacagaccc cacaaaagta ttaacgaata ccctcggata tgatcgagaa
     6421 gtccgaaaaa tctctttaag tcagcttaaa ggaagtaatg aagggctaaa agttaagttt
     6481 gctagcaata tggatgaaga agctaaaaaa gctacagtta aaaacattgc tgatttttat
     6541 cgacaaaacg gtggactact tgtggaagaa aacggtgtag aaatcgaacg tttacaacga
     6601 gagctggtag acagcaagct tttagatact gataaagtat ctcgctccag aatcgctatg
     6661 gtctacaatg tgccggagca tttcatcggg aataaccagt cgagttactc ctctcaggaa
     6721 cagctcaata tggaattttt gacgtacaat ctagtaccga ccgttaatca atatgaagcg
     6781 gaactaaata agaaaacact atcgagaacc gaaaaagcaa aaggttatcg atataagttt
     6841 aatgtcgcaa gtttgctaag agctgacact caggcaaggg gacaattcta tcaaatcatg
     6901 cgacgaggcg gagcatactc agcgaatgat gtccgaagat ttgaggactt gcaaccgata
     6961 aataaaaccg gtatggatga ttaccatatt tccggagacc tatatccaat cgatatggat
     7021 ccaacattaa gaaaaacaac ctcgtctaaa agcgtagccg aaaacggtta ggttttttta
     7081 gtttgcaccg aagggaggtg gaaaaatgaa aaaagtgacg ttaagcggcg atgtcgtgga
     7141 taacgatacc gcgtggcttt atgactggtt tgggatcaat tgtatctcac cagggaaaat
     7201 ttctgccgct cttacagaag cagcggggga tgaagtagaa cttgatatct catcgaacgg
     7261 tggggatgtc ctagcggcaa gcgaaatata taccgctatc cgtgcctatc cgggaaaggt
     7321 atccggaaat gttgtgagta ttgcagcaag cgctgcaagt gtaatcgctt gcgcttgcga
     7381 accgcttaga atctcaccta cggcacacat catgattcat aacgcatggg tgaccactag
     7441 tggcaacgct gaggaattaa aagccaatgc agaaatgtta agcagtgtgg atgagtctat
     7501 tgttaatgct tacgagatca aaacaggact cgatcggaaa aaacttgctg atttaatggc
     7561 gaaagatact tggttaaatg ctcaaacagc agtagcagaa gggtttgcag atgaaattat
     7621 gtttgcagaa gcaccagtaa cggtactcaa tgcctctcaa ccggttattc caaaaaacgc
     7681 agtaactaag ttgaaaaatt taatactcaa agcggaagca ccgcaagaag aaacactctt
     7741 acagaaaaaa ctaaaagcct taaatggagg gaaaaacgaa tgaatttaga acaattaaaa
     7801 aatgcgtggg tcgaggcggg aagtaaagtc tctgacttaa atgcacaact caatgcagca
     7861 ttggttgacg atgaaaaaac agaagaagat gtagtaagtt tgcaagcaca agtaaaagca
     7921 gcacgggcta aacgggacgg attgaaagag caagtggcaa atatggaagc cgaacaagtc
     7981 ttaaacgtca aaaaagaacc attagataaa aaagatgaaa acttgaaaaa caagtttatc
     8041 aaagacttta aagcgatggt caatggtgat cctgctatta tggctacttt gacatctaat
     8101 acggatgaat ctggtaatgc tatcggattg actattcctg tagatgtgca aacgactatt
     8161 catactttgg ttcgtcggtt tgactctttg caagaatacg taaacgttga aaaagtgacc
     8221 actaccagcg gttctcgcgt ttatgaaaaa tggtctgata ttacaccgtt gactgctttg
     8281 gatactgaag acggtgaaat cccagcaaat gatgatcctg cactttactt gatcaaatac
     8341 ttgatcaaac gctatgcagg tatttccaca gtaactaaca gcttgctaaa agatactgcc
     8401 gaaaacattt tggcatggtt gtctaaatgg atcgcgaaaa aagtagttgt tactcgcaat
     8461 acaaaaatct tggcagctat tgatggaatc aaagcggcac aaaagaaaga tgttacagat
     8521 gttgatggaa ttaaagatat cgtaaacgtc caacttgatc cagctatcga agctacatct
     8581 atgtttatta caaaccaaga tggcttcaat gttttagata aagtgaaacg tgctgatgga
     8641 tcttacttgt tacaaaaaga cgtagcttct gcaactggat ataccttctt aggtaaacca
     8701 atcaagaaaa ttgcttctcg tttcttgcca aacaaaggta cgcaagctac tcctaaatat
     8761 ccactgtaca ttggtgatct gaaagaagcc gttacattgt atgatcgcga aaacatgagc
     8821 ttgctgacaa cgaatattgg tggtggagct tttgaaacag acaccactaa agtacgcgtc
     8881 atcgatcgct tcgatgtgca actagttgat gatgaagcgg ttgttttggc tacttttaca
     8941 actattgcga acgagacacc ggcgaaagtt taaggagctg attccttatg attcttgatc
     9001 ctaaaatgga tttaggcgaa atcaaaaacg cactaaagat tgataccgat gatgacgatg
     9061 tggaagtaag ccgtgcggca caagctgcaa ttgcatacat tagaggggct atcgggaatg
     9121 ataagccctc tttttataag caagaaaatg aaacggttga tctgattaat ttagctatcc
     9181 tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg aacttgcgag
     9241 agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagttat ttgctttttg
     9301 tggaggagga gtagcgtatg ccccttatcc aaacaggaaa tttaaatcaa cgcatcaagt
     9361 ttgtccgaga tacgacggtt aaggatgagg acgggcaagt tgtcccgact tctacaacca
     9421 ttcttacttg ctgggcaagt gtgcagacac aacgcctgaa cgatattaag acgtcgattg
     9481 gtacggcttt ggaaggaaca ctgacgttca ttatccgcta ccaacaaaaa tcagagctga
     9541 ccaatgatat gaaagtgcgt tggaatggaa aaacgtttga aatcattacg attacgaaag
     9601 gcgagtttgc gaaggacttc acgacaatca ttgcaaaaga ggtttcaaaa tgagtgtaga
     9661 agtcgatgca accgaagtgt acaaagcgct tagggaagta aaagcaaacg ttcaacgagt
     9721 ggaaagccca gcacttagaa aagctgggga gtacgctcaa gaagagttac gacaaaacac
     9781 accttactgg gatggaacga agtcaaacgg taaacgtggt tcgtatatgc aagaacatgc
     9841 taagaaccat gtggttacaa gctcgataaa aaacggattg gtagaagtcg gctatgacaa
     9901 agatgtttct tggcggatgc actttatcga gttcggaaca atcaaacaac gtccaaaagg
     9961 tttcgtacaa aaaacacaaa agcaaatcga aaaacaagta acacaaatca ttgctgacga
    10021 agtaaaaagg aggctaggac tttgaaaacg gcagtatcac aagtctattc aattctgaat
    10081 agcaatgaaa aaacaaagaa cattgatttt tacaccaata gtgttccgga atcagctcaa
    10141 acagtaccta gccttccagt tggcagaatt acagagatat ccggcaacta tgaagatttt
    10201 gcaagcaaca atcctttgac cattcaattt aacgtacagg tagatgtatg ggtgtcaacc
    10261 atgaaagagg ttgatgcctt ttattttgcc cttgatgagg ttatgagggg gaatggttgg
    10321 caatgcgcat acacggaaca aacagatgac gaggacttgg aaggtgcaaa gcggattatc
    10381 aaacgatatg tagcaaatat ttcactaaat taaaaggaga gaaaatagat ggcaacagta
    10441 ggattcgaga gcgtcatttt tggcgtaaaa acaggtgcag gcggcactct aaaagaatta
    10501 gtagcagata agtcgaaagg cggagcgatc gaagctaaaa ttactggatt aggcgcaact
    10561 tctaacacaa catacgcttc aaacgtaccg ttcttcattg caagtaaagg ggtttcgtcg
    10621 ccaaaagtta cgcttgacgt ggcagactta atggataacg gcatttacag cgaaatcatt
    10681 ggtgctaaaa ccgtggatgg tgcaaatgta attggttcag aaactgaagc gccttacgtg
    10741 tcggtagtca tggttacagc gaacaaagaa ggaaaacgct tgttcatggg attgacaaaa
    10801 ggaaaattca gtcatccaga tatcgacatg aaaacagctg aagacaaagg ggtagaattg
    10861 caaaccgatt ccatcgaagg ggaattcatt tctgatgaac gtggctatgt atacttaaca
    10921 gccgtagaat cagaaggaat gaccttacaa aaattcaagg acttggtaaa taacaaagag
    10981 ggggagtagt taaccctgca tctacaccaa cgacagatac agggacacca aaagaaccag
    11041 aaccaaaaat tgatacacaa ggttagccat ttttggctag ccttattttt tgtaaaaaca
    11101 aggaggaaaa caaatgattg aattgcaatt gaaacttgac ggaaagaaaa aaacattcaa
    11161 acaacaagat atttccgcac gtgcaatgcg tgagtgtatc aaattttacg agaaagcgga
    11221 aaaagcagac ctaactgatt tagaagcgat tgattcaatg attgcaatta cagcagatat
    11281 tttccaagat ccagcagtta catttgatgc tattttagac ggtttgactg cgagcgagtt
    11341 agtaccggca ttagaaagtg tttttgaaca aataaatgaa ctgggaaaca atgaaaaaaa
    11401 gcagacggcg agcaaaaaga aataagtttt tctgaagcta ggaaagcaat ggatcaaatc
    11461 tacaaagatt taatcgaagc aggttggacg atgagagatg tggacgaagc cgactatcat
    11521 tatttgttac acctttttgg agaagtggag agtggcgaag aatatgtaga tggtgctgat
    11581 ttcatcaaac aatttttatc ggctgaagac ttagtaaaac ttgaggaagg aggtaaataa
    11641 tggcaggaaa aggacaaccg gcaggaaata tcaagctagg gattagttta gatagcacta
    11701 gttttggtaa cacgctggac gaaatcaatg cgaaagtcaa acaagctgag tcgaatatgc
    11761 gtgccaatct aaaggcttat gattcagcag gacgttcata cgaagcactt agtcaaaaga
    11821 cgaaagactt gtctacggtt atggaagggc aaaacgccaa agtaagagaa ttaacaaagc
    11881 gccgtgatga agcgattagc aagtatggcg aggaatcgaa acaagttgct aaccttaaca
    11941 cacagataaa caatgctacc gcaaaatata atgcttacag tcgccagttg aacgacacaa
    12001 aaaaagaatt ggtgtattcc aaaacagccg tcaatgattt atctaatgaa atcaaagaaa
    12061 atgaacgaca aatgaacgcc gaagtaaaag cgttgaaagc cgctggtgat gaatctggtg
    12121 cttttgaagc aaaacaaaaa gggctagcca aacaaacgga attatccgag aaagctatcg
    12181 aagaacagcg caaagttgtg aaactgatgg ctgatgagtt tggcgattca gcaaatgaaa
    12241 ccgaagatgc aaaaagggca ttagaaaagt tagaacgaca aagccaaata tctagcaggc
    12301 aattagaagc actcaaaagc tccagcgatc aatcaggaaa agaaatagaa gattttggcg
    12361 acaagtccac aaggtcagct aggaaactgg acggactaaa agacaaatta agctcgctaa
    12421 aaagcgcatt ttcgtttggt gcagttgctg gattagcgca taacgctatt agcagtgtag
    12481 taagtggcgt gcaaggtttg gttggcgaag cagtaaacgc atcggattca ttgatgaagt
    12541 tttccaaaac catggagttt gctaactttg ggaagtcaca gatagaaagc tcgaaaaaag
    12601 aaatgaaaga ctacgccgat aagacggttt atggtttaga agaaattctg aacacaaccg
    12661 cacaattggc atctaatggg attcctaact atacagaact aaccaaggcg gcaggtaact
    12721 tgaatgccgt tgcaggcggt tctagtgata cattcaaatc cgttgccatg atgctaacgc
    12781 agacggcagg agctgggaaa ctaacaactg aaaactggaa tcaattagca gatgcgatac
    12841 cgggtgcttc aggattgtta caagacgcta tgttgaaaaa cggagcttat acaggaaact
    12901 tccgtgatgc aatggcgcaa ggtcaaatca cttccgacga gttcaaccaa gcaattgtac
    12961 agttaggtat gaatgacgga gcagttaagg cagccacttc tacagacaca ttgagcggtt
    13021 cttgggagca gatgaaatcc actgtaataa atgggctaca aagtattata gaaaaaatag
    13081 gcgttgaaaa tatcactggt ttcatcaaca gagtaacaaa agggattgaa aattctattc
    13141 ctaaaattac tcaatttata ggttggttga gagatattgg aacgtggatc gttgaaaata
    13201 gagagccact aacatggatt gtcggaatca taggcggaat tacattagca gtaaaagcat
    13261 tgaacgtagc aagtatgttg ctggcaatta ctggcggaac attggcagcc ccttttgtgg
    13321 cgattggtgt agcattaggc gcactagcag gtgctttggt gatagcttat acaaaatctg
    13381 aaacatttag aaatatagtc aatgcggctt ttacagctgt gaaaaacgta gttatgagcg
    13441 ttatcaataa tttggtggaa tactacaaaa tgttgtggag cgtgttgcag tggctttggg
    13501 aaaaaataaa agaatgggct tcatggattg gcaataaatt cattgaaatg aagaacagcg
    13561 ttgtgaatac ggttcagaac ctatggaatg gcgtgaaaaa cttcttcagc aatggcgttg
    13621 gagatacgtg gaataaggta gtcggttggg taaaaaacat tttcaacaaa gcaactgaat
    13681 tgaagaacaa cgtttctgat gtaatcggta acctgtggaa tggtatcaaa gacacattcc
    13741 gtagaggtat cgatacggta ttcaattggt tttcagaact accaacgaag atgaagaatg
    13801 ccattattgg cggtaaaaac gccattgttg atgcgttcaa aagtattttc aacgcagcac
    13861 ttaaagcgat aggtaaacca gttaacgcaa tcatccatgg agcttcatgg gtactagaaa
    13921 aattgggcgc agaacctcta caagaatggg atgtaccaca atacgctaca ggtacaccag
    13981 caggtgggca cccaatcaat ggtccaatga tggttaatga tggacgtgga gcagaaacag
    14041 ttatcacacc agatggtaga gccttcattc ctaaaggacg caatgttgtt ctaaacgcac
    14101 caaaaggaac gcatgtcttg acagcagaag agaccgcgca acttcaaggt tctaaagctc
    14161 ctaagtatcg ttacaaaaaa ggtactaact tctttggtaa catgtgggat agtgtgaaaa
    14221 aggttgctgg taatgtaggt cacacaatta aaaacgtagt aggtgacgtg tgggacttta
    14281 tttcagaccc gggagcgtta gctagaaaag tacttggggg gttagatgta ttaggtggat
    14341 tgacaaagta tccattagaa gtaggtaaag gcatcctatc taaagcaaca agtgcactga
    14401 ctgaaaaaat cactgggttg ttctcatctg gtaacttaga tacctccgta ggaacaaatg
    14461 gcgtctataa atatttggct gatgttgcta agtctgtgat gaagaaattc ccaggatttg
    14521 tggcaactag tgggtataga cctatagacc aggtgaccct tattcacatg gtaaacgtaa
    14581 tgccattgat attgcattac caggcgtcac aggaggctca cctagataca cagaagcagc
    14641 aaactacgct tttgacaaat tcgcttccaa aattggttac gtaatcacta atgggaaagt
    14701 tcgtgaccgt tcaggacaat caggtcaacc agcaactggt gcatgggagc catggcccga
    14761 tggagatcac tatgatcatg tgcatttaaa cggtgtgaaa gacccacaaa acactcaaat
    14821 ttcaggagat agcgtgggag gcagtggggt agaaagatgg cgcaatgtag caattagagc
    14881 gttgaaaatg accggtcaat acagtactgc aaacttaaat gcattactaa atcaaatgcg
    14941 tacagagtca aatggtaatc ctaatgcagt taacaattgg gatattaacg ccaaaaatgg
    15001 aacaccatca aaagggttgc tccaagtgat tgacccaaca ttcagacagt atgcaatgcc
    15061 aggattcaac agcaatattt ttgacccact atctaacatc ttagcttcaa tcagatacgc
    15121 actatcaaga tatggctcac taacaaatgc ctatcgtgga gttggttacg caaacggtgg
    15181 aattgtaaac caacatcaaa ttgcggaaat cgcagaagga aacaagccag aaattattat
    15241 tccgttagat aaggctaaac gatcaagagc gatgcaatta ttgtctatcg caatggacaa
    15301 actaggagtt tctcctaaaa cttatggaaa tgcgacaact gtttccagtg attacgaaat
    15361 cctagaagcc attgaaaaac aggcggcaac aacgaatcag ctgttatcgt tattgctggc
    15421 atttttcaaa ggaaatagcc gaactgatag agatttagct ttagatattc agaagatctt
    15481 ggttaggagg atgtagatgc gaactgtatt actgagaaat agaataaacg aagaaattga
    15541 tttgtccaca gaagactatt ttgctactgg gttgagtaat atggggtttg aagtaaaaaa
    15601 agaacatgtg ggacagtggg ggaatttcag agaaagcagt gagagcgttg aaatatctga
    15661 atttcagtca tctgtaatca tttctgtgca tgggtttcga gaaaaagaac tgtacaattc
    15721 acttgtgcag ttcctatcgg aaggtccgtt tgaactggaa tttgcttttg acggcgaaac
    15781 aatggtaaga agatgcagtc taaaatcttt aagtaagact gaaatcgatc cgaaaacatc
    15841 gttactaaca gacactttag aactatattt cacatcaaac tggtattccg taaaacgcga
    15901 aaaattaatt cagcgaccga atgtagtgaa aacacgtggt aaggtttttc cgtataaaag
    15961 atcttatatc tacactcaaa acttgtggga gaaaaaaggt gtattcaaat tcaataataa
    16021 ttctgtatac ctaacgaata gcaaggaacg gatgtccccg ctaaaaattc gtgtgattgg
    16081 gaagtgttca aatccgtatt gggaagtaat ccaaaactca caaatcatcg caacggatgg
    16141 atacttcata gatatgactg aaacacagac tctagaagta tcaagccttt ttgaagatac
    16201 aacggcgatt ttaaaagata ttgcaggggt agaatcttct gtctaccaac aacaggatta
    16261 tacaaaaact aattttgttc aggccccaac tggagaattc agcattgtgt ttcatgttgg
    16321 gggggcagac gtagagattg agttatacga ggagcgtgat ctgttttgat tttagcagtc
    16381 acgctttttc atcgtgattt aatgctttat aacgaacatt tattctctaa cagttttgaa
    16441 tttggtgtgg acgagatcaa cgaagaggct agtagcttta cgatggataa gtacgttcct
    16501 gtaaaaacag gcgattttct actagcaaaa tatattccta gtggaaaatt tgcatatttt
    16561 ggtgtaatta cgtcgcagga agacgaaaaa atcagttgta aaagcttact tagtttagct
    16621 gatagtgaga taccgactgc acgtgtgtca ggagataact acgaagagca tatccggcgg
    16681 ttgattgaat actatttgct gaatgatcca acgaaacaac taaaagatat tttagacgtc
    16741 aaagcagaaa gtgcgacctc tcattcgtat caagctaccg atacgaataa acacaagtta
    16801 agtgcgtata ttctcaatgg tttcaaaaaa tacaacgtaa aatggtattt caagggaatt
    16861 caaaacagaa aaatttatac aggcatacgt gctgtaaatg aatcaattta cattaaagac
    16921 aattcttctg aatttagcga ttgggatgtg tttgttcagg cgccgggcgc tggaaacgag
    16981 aacaagctat taatcgttga taaagcaatg aaagatatag agaaaccgat aatactgtca
    17041 acatggtatt tggacgaaga aaacaatttg acacaagatg gatcgaaaga aaatatcacg
    17101 aaaccaactg tcaatttggt taatatctac gatcaaaccg cagaagataa agcatcctac
    17161 gaagatgtgg caaaatcaga gttgaaaggc aatacgtatt cgcatgaaat caaagtgaac
    17221 gttgtaagaa atgcaaaaaa tttgaatgtc gaaacgattg aaacagggat gtttgccacg
    17281 atttcttata aaggaaagat atacaagtcg gttttgacag cttggcgaat atcaagtgat
    17341 aaggagtttg tggaattgac tttcggaaac ataagaagtc gttttatgga ttattttgaa
    17401 gataatgggg gataaaaaat ggttagcaat gtggatggat atcaatttga aaacgtgaaa
    17461 gtaagcgcag aaaatgatgc tagactttat cacgttttat ataatcggaa aaatcaggtt
    17521 attgatggtt acgatcagtc tatgaattta tcttcaagcg gattaacagt aaaagttgct
    17581 gcaggagcag cgattattca aggtcgtatg gtcgttgttc gacaagaaga aagtataaca
    17641 gttccagcaa actcaagtgg ttatatagca ttaacagtgg atttgacaca agaagttata
    17701 cctggatcta ttcttccaga atcggaagaa tatgaatgga ctaataatca agtcaagcta
    17761 gaatttataa caaaagttat aaaaggtaat ttaaacaatg gtgataaggt ctataattta
    17821 ccactatgct cagttaattc tactggatca actgtttcaa tctcaaagat atcggatagt
    17881 tacgagctga ctctctctaa aggagaaatt ttgtggaggg ggactgcgtt aatgcatgat
    17941 actcaaactg tccaaccttc aaaaaaaatt tggcagacag tttcaggttt tttgttagta
    18001 tggctcccat acgaaaacgg gcaagcaatt gaggatagat atgtaactac gcctttttat
    18061 aaggagcgtg taacttttac taatgtttta ggagaaattg tttcaggatt tgatgactat
    18121 cacaaaaagt ggtttagtaa acgaataaac tataattcga atactaatat atttacaggt
    18181 gcagcaagca atgcaagtgg agataacgca aatatggtgc ttaggtatat agtttctttt
    18241 tagttaggag gtatggaaga gtggcaaatt tagaaattaa attatctgca aataagagac
    18301 agccttatct acgtcaccgt gttgttggta gagttggtga tggggggctc acaacaatca
    18361 atgtacaact tcttgaggaa gatgaaatta caccttttgt aattaatcta aacggtactt
    18421 tgaaatttgt gggcgaagtt tcaaacggta actataccga gggagaacca gaaataatcg
    18481 attcgactaa tgggttaatt agttacacgt tcactaagtc aaatttcagc acgagtcatc
    18541 aattcaaaca agcatatttt gaatatgtag atcctaacgg caaaaaagta acttttcaga
    18601 acttcatcat agacgtgtta gaacgagtgg atattaattc ggagcaagcg aaatactata
    18661 tttcttcatt ggaaaaatta cagagtgaaa tgcaaaccac tttcaatcag ttcattagtg
    18721 ataaacaggt ccaatacgat caaatctact cgaaatataa cgaattagta agattgataa
    18781 atgaatcaga taagcaagta aatgatcgta ttgatcaaac caatcagcaa atcggcgatc
    18841 tcggcaagct gaaaaagatg tacagtaaca gcatcgactt tgggggctat gattattcgg
    18901 ggaatccgaa tttgttaagt aagctatcat acgacttaat tgaaaatcaa aatacttcag
    18961 ctggaacact ttctaagggt gaaaactcgt ttaaatataa taagatatca gctgaagtgg
    19021 aaggtggagt agagttatat tataaacgaa gaggtatagc taactggtta ccctctaata
    19081 aaacgcttgt aatgactgtt aagcttagag ctggagcgga ctatagtcca gttgacggaa
    19141 aacttatact gattagatat aggtatgttg acagtggaac tggcaagatt gttttagact
    19201 tacctattaa cagtaattcg ataactcagg aatggaaaga gtttagtatt actggaacta
    19261 ctccaacatt tagcccacaa gcataccatc cttggataca atttagggct caagatgggg
    19321 tacttgggga aatagaaatg agctatgaca tcaaaatcga agaaggctct acagctacac
    19381 catatcagcc taacttactt gatgactctt actggctagg taaaacaccg ttgggtgaga
    19441 atttgataac gaatgccaag ttcccaatta taacacgaag caatcctatt tctggttttg
    19501 atatttcaga agaattaata ataggtgaaa cttatacggt atctttaaaa gggactaagc
    19561 cagctactaa agaattccac ttatactacg gagaagcaaa ttaccagcag ttgcagtatc
    19621 aggcaacttt acttcctgtg gaaggattaa cagatgtgtg gagtgccact tttacagcca
    19681 gaaatactag tgaaacgaca aatttactta gagtagcgtt atggcaaaaa cctaattctg
    19741 caacatatga tactgttcaa attgactggc tcaagattga aaaaggcgac acacgcacac
    19801 caaatatcag tcagtttaaa tactttggtg aaggcttgaa agacagtaac aatccgaacg
    19861 actacagctg ggatgtcaca ccagagtata ctgaaaaaag cttgaataat acggttagtc
    19921 tgactgaacc gcaatccatc gaaggattga aaaatttcga agatggtatt caatcaaaag
    19981 gaaaatctgt attgacatca gacgacaaca aatatgaagt cgtaacctta acagttacaa
    20041 acgggaatac cggatcagca aagctttatc gtgaaggaaa aaccgtcact atttattttt
    20101 ttgcgttgaa tgg
//
LOCUS       DACEDO010000052        19845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEDO010000052 DACEDO010000000
VERSION     DACEDO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.052"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..935
                     /locus_tag="IWO52_001965"
     CDS             156..935
                     /locus_tag="IWO52_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356227.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            981..1925
                     /locus_tag="IWO52_001966"
     CDS             981..1925
                     /locus_tag="IWO52_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0356228.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1941..2720
                     /locus_tag="IWO52_001967"
     CDS             1941..2720
                     /locus_tag="IWO52_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0356229.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2732..3430
                     /locus_tag="IWO52_001968"
     CDS             2732..3430
                     /locus_tag="IWO52_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0356230.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQVLAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3458..4222
                     /locus_tag="IWO52_001969"
     CDS             3458..4222
                     /locus_tag="IWO52_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0356231.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            4235..6052
                     /locus_tag="IWO52_001970"
     CDS             4235..6052
                     /locus_tag="IWO52_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0356232.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAVFGSLSASAVSSIIILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYVETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAARVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6063..6695
                     /locus_tag="IWO52_001971"
     CDS             6063..6695
                     /locus_tag="IWO52_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0356233.1"
                     /translation="MKAIIVGAGGFGKEIAFLLQSVSRYELVGFVDDSLKMQNQELLE
                     KPILGTIDLLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSVFPSVNISGHVILGD
                     KNEIGVGTKIIQNLSIGNTNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            6697..7815
                     /locus_tag="IWO52_001972"
     CDS             6697..7815
                     /locus_tag="IWO52_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0356234.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNEKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHNFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            7808..8425
                     /locus_tag="IWO52_001973"
     CDS             7808..8425
                     /locus_tag="IWO52_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0356235.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKILRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            8433..9647
                     /locus_tag="IWO52_001974"
     CDS             8433..9647
                     /locus_tag="IWO52_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0356236.1"
                     /translation="MPLNILFLTLLDFQSLEESNIYTDLMSEFVKNNHNVSIISPTER
                     KKRIKTHLIEINDKCEILKLRIGNIQKTNLIEKGFSTIQIERKFISGINQYFSNKKFD
                     LVIYSTPPVTFAKVVKFIKKRDNCKSYLLLKDIFPQNAVDLNQLSKNTYIYKYFRKKE
                     VSLYEVSDYIGTMSEQNSQYIRKHNKIDEQILEVNPNSIRINENKIGSPLDKEEIRNL
                     YKIPKEKTIMLYGGNLGKPQGIDFLLNIISSLEKDNKILLLIIGSGTEYGRIRKHINT
                     NNIKNTILIDSLSKNEYKKVESIADIGLVFLDKKFTIPNIPSRILGYMESKKPILAAV
                     DKHTDLNEMIKTGKFGYCSEHGDLNAFLSNMERLKDEKIRNELGKNAFIYLKNNFDVK
                     RSYSIIMNHFEL"
     gene            9663..10685
                     /locus_tag="IWO52_001975"
     CDS             9663..10685
                     /locus_tag="IWO52_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0356237.1"
                     /translation="MFKDKILLITGGTGSFGNAVMKRFLDSDIKEIRIFSRDEKKQDD
                     MRKKYNNDKLKFYIGDVRDINSVKNAMYGVDYVFHAAALKQVPSCEFFPMEAVKTNIM
                     GTDNVVTAAIEMGIQKVICLSTDKAAYPINAMGISKAMMEKVFVAKARTVTDERTMIC
                     GTRYGNVMASRGSVIPLFIDQIKNGKPLTVTDPGMTRFLMSLEEAVELVVFAFQNAKA
                     GDIMVQKSPASTVGDLAQAIKELFHADNEIHIIGTRHGEKRYETLLTKEEYLVAQDMG
                     DFYRVPADTRDLNYDKYFVEGSKELTQEGEYNSDNTQRLSVEQIKERLLELDYVRKEL
                     KEWQNQ"
     gene            10682..11791
                     /locus_tag="IWO52_001976"
     CDS             10682..11791
                     /locus_tag="IWO52_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HAQ0356238.1"
                     /translation="MNILVTGSNGFIGKNLVAELKNEGFTDILSFDRDTPVHLLDEYC
                     EKADFVFHLAGVNRPKDDAEFMTGNFGFTSELLEKLKEHGNTCPVMISSSIQAELNNS
                     YGKSKKAGEDLLKKYQKETGAPVYIYRFSNLYGKWSQPNYNTVVATFCYKIARDEEVT
                     VNDPSAIIELCYIDDVVKELLHCLNGQPYTENGYCAVRETDEVTVGELKELITSFKNS
                     RTNLSVPNLARRVEKNLYSTYLSFLPEDEFSYFLKMNVDNRGSFTEFLRTKDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGEGVIRFRKVDSNEIIEYFVSGEKLEVVDIPVG
                     YTHNIENLGSTDMVTVMWVNEPFDPEHPDTYYVEV"
     gene            11795..12919
                     /gene="wecB"
                     /locus_tag="IWO52_001977"
     CDS             11795..12919
                     /gene="wecB"
                     /locus_tag="IWO52_001977"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAQ0356239.1"
                     /translation="MKKLKVMTVVGTRPEIIRLSAVINRLDRSEAIDHILVHTGQNYD
                     YELNEVFFEDFKLKKPDYFLNAATGTAIETVGNILIKIDPILEEVKPDAFLVLGDTNS
                     CLCAIAAKRRHIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINLTYSDIAREYLLRE
                     GLPADRVIKTGSPMFEVLNSRKDDIQHSDVLNRLGLKEGQYFVVSAHREENISSETNF
                     LHLVDSLNTIAETYQLPIIISTHPRTMKMIEAKEIKFHELIQTMKPMGFNDYNKLQIN
                     AKAVLSDSGTISEESSILNFKALNIRQAHERPEAMEEASVMMVGLEKERIMQGLEVLE
                     TQQKDTLRHVADYSMPNVSEKVLRIILSYTDYVNRVVWGR"
     gene            12916..13971
                     /locus_tag="IWO52_001978"
     CDS             12916..13971
                     /locus_tag="IWO52_001978"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0356240.1"
                     /translation="MRIVVNDIAASEGGAMSVLRDLHSDIISSGDENEWIFLLGEKFI
                     EESDNIKVLTFPKIKTSWIKRLDFDFISGKKIINKLQPDIYLSLQNTATIGVKAPQYV
                     FLHQPLPYQKEKNFSFIKKKEFKMATYQHVIGKIYSILFKKTKATIIVQTQWMKENVS
                     SILNNKILKVDTKINIDENIINKQINENKRNMKHFFYPASNIPYKNHDFLIESFSKCK
                     DKELRLFLTLSRDEIDIKDDRIILLGKVPREKVFDIYSKSNLIFPSSIETLGLPLLEA
                     QCFMSPILALETEFSKEALSGYPHKYLFKLDNLDNLSHLIEKISTSSIVFNVENVIYK
                     KENRVSILDILKGENIY"
     gene            13968..15182
                     /locus_tag="IWO52_001979"
     CDS             13968..15182
                     /locus_tag="IWO52_001979"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0356241.1"
                     /translation="MKKFVVAHPAQQHSYKIAEALIEKEMLFKYITSVYLKKGSILNK
                     LLNIIPGENKLRAKGRHSDKICDEEVLLINEFSGLLLLILQRVKFLKHLYKKYWDFHN
                     KKFNKKLIKYINSNIDEISGVIVFDQVSFDFFKKKINIPIILDMSAPSVVFMKSQFEK
                     YGISTLKYDALLKKNNFEINSADFFIGASSITERSLLNMGIEKKQIKIFPYGVNIVKE
                     NDKLMNLRTRKKRLKFLFVGRVTLEKGIGVLDSFFEKFGIEDAWTFAGDYSDSKKFYE
                     KYEDKVQFVGHVSKIKMFELYRDNDVLVFPSLADGFGLAVIEALSNGMYVICSDSTGA
                     KDIIMEGKNGDVFLTGDENDLNNKIQYYKKNINKIDRLKIKETVNDLTWERYNKNIIK
                     SLEYFTVGMNNE"
     gene            15175..16290
                     /locus_tag="IWO52_001980"
     CDS             15175..16290
                     /locus_tag="IWO52_001980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356242.1"
                     /translation="MNKLLMMLNFFYLYVYMIDAYGIINNDLYLRMVLIILFIMIGLI
                     NCIKDIMNKIYSKDNLFFTLYLIYFLSTQYMLWNIDISQLIMIIYTPMMFIAFSRFHI
                     DRYFYEKFTFSYILLMTFTYFYTRFFLNINNGLVINSCYYLLLTVPIVYLFVNPYRKN
                     ISFLLISIIILFSMKRTALITLALIIFIELILNVLNKKRISLRRLAFLIPFSLFIAAL
                     LIKINKIISSLYDNNIFMRFYSGDTIGESRGDILKLLLEKICTNSNIYFILLGQGINS
                     TIYYTGGLTAHNDFLEVLFDFGIFGLLLYIMIYIIFIKKIRIITNVKDKRSLLYTMVI
                     LMLVSVSSHLIFIPSYVSLLIMNYNYLKNKNYFSKEN"
     gene            16290..17384
                     /locus_tag="IWO52_001981"
     CDS             16290..17384
                     /locus_tag="IWO52_001981"
                     /inference="COORDINATES: protein motif:HMM:NF016145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAQ0356243.1"
                     /translation="MKKIGILTFHRAENYGAVLQTYALQEYISKMSSNYEVEVIDYRS
                     PFIEKYYKLLSFKAFTNIKSLKRIVYVLLYNGTLKSKRKIFDNFIDKFIRVSLSEYDD
                     KTIRGLDDKYDLFICGSDQIWSPYSAGFDPIYFLDFVKKRNKKNAYAASFGVEIVPEE
                     TIKEYSRRLKNFNVISVREEIGKKIIKNLIEEDVPVSLDPTLLLSKTEWDLIIEKKRK
                     ISEKYLLIYLITETREIIELAKKVAREKKLKLVYINDNIYKTKGIYNYNNCSPSDWLN
                     LFYNADYIVTNSFHGIAFSINFEKEFSVQYLPEPAKVNSRINNILDNYSLKWRINYDG
                     NEKINYSKVNKILVEDRVASQQILKDIMEY"
     gene            17394..18377
                     /locus_tag="IWO52_001982"
     CDS             17394..18377
                     /locus_tag="IWO52_001982"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0356244.1"
                     /translation="MKPKVSIIIPAYNAEYTISRALESALKQTYSNKEIIVIDDGSKD
                     RTLAICKEYQLNNHEIFIFSQKNSGVSVARNLGIRKADGEFIQFLDADDAMEPNMTEK
                     LIEVQRNNDSDYVICGLTIIKDNNIVRTPHLKERNINFINFEDYKYIYPLLAAPCNKL
                     FKKELIVLFEKNFSNGEDLLFNTEYLTKIKKISIIEDCLYKVYLDNENSLNKKMSIST
                     VKSLLLINEYEKEIMNKLFDNDNVLEFNVFNKFKIIQTSLQKIVNNEEKDDVIELINN
                     LRSDSQFLTILEKTNMKNMKSNLIKFCFSYCDSQTIYQLFFLISKVRGYFQ"
     gene            18374..19810
                     /locus_tag="IWO52_001983"
     CDS             18374..19810
                     /locus_tag="IWO52_001983"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0356245.1"
                     /translation="MKDRVKNVIYNAVYQVFIIVIPILTMPYVSRKLGAENLGINTYT
                     QSLVLLLGLFINSSILQIGAREIARQDRISRTKTFITLWLLQVLCGLVIIIVYILIIM
                     GSKYYYYYLLQLPFLISYIFDISWFFVGLEKIKNVVFRNMFVKVCTIFLIFLLIKNKN
                     DLSIYIIIISLGSLVGNFIFFFSLKKYLDFDKIVGLDLSNLRPFINSIVILLVPQVAI
                     QLYQYIIKFSIENSTTTTELSYFDQSQKIARIIIQLVSSISIVLMPVIANLDKNKSRN
                     ALNKIIQLSVNYSLSIAVIFTILLVIISPTFVPIFFGPEFKEMIPHMIISSFIIIPIC
                     VGGVFSNQLILGMGLYKYYATPYLIGGSIGILICLIYKDKMSGLIGCIVLVIVESLVC
                     FFRIIFANKIIDIKENLQGTIKEFFLLFLLLAEVYIVNTIFTPSPSALLNLFYMMIII
                     IQYFVLSTIIKTETAKDLSKRLKSFLIK"
BASE COUNT         7402 a         2801 c         3608 g         6034 t
ORIGIN      
        1 aacagtttgt ggtatgatta tatagggaaa acttgtttac aaatatttct gtgaatatat
       61 tagtttaatt atgtttttta aaataaaaag ctgtcatttt tatgtaagtg aattatatct
      121 taatttatct atcatcaata cttaggagtg aatctatgga aaagccattc gtcaagtcat
      181 tgactgctgt ggcggtaggt gcaggtgcta ttgcgatctg tttgtttggt taccatatca
      241 ataatcagcg acaacatcag caacggatca attatgcaga atctgccatc accaatcaaa
      301 aagacacctt gacttcttta tcaaaagaag tagacaagct gtatagtaca aaagaaaaaa
      361 tttttctaaa ccctgaaata acagaagaaa caatcacaag tttgtcaaat aagctatcct
      421 ctgtcaaact ttcagcagat gattacgaca tcaaagaatc agaattgcct aaagaagctg
      481 ttgcaatcca agatgagaag aaagcaatcg tgacacagtt agatagtgca gaatcaaaac
      541 tgaagatcca gacagctgtc aacgatttat ttactaaaaa tgtatcaaac tggcagcaag
      601 ctgtagacga tgtcatcatc aaagaaaagg tgacaagtgc agatgtcgct cgtgtacgtg
      661 aaaatatgag cttttttaaa gatagtcctt ggaaaacggt tgttatgcaa tatctaggat
      721 tcgctgatac tcagatcgcg caagtcacgc aactagatca gttattcgat acgatgttga
      781 aggatggtca agtgactgcc accgctactt acgatcaata tttgacggtg ctcagtcaaa
      841 tagagcagat ccggaatgaa aaaatacgcg cagcatacgc aacaaaagca caaactgttg
      901 ctcagcaaat gggctatagc aatataagct attgacagtt aaaacgttga ttcaagcaag
      961 gttagcaaac ggaggaaact atggcaagac gaagaagaaa atcaagaggg aaaaaaatat
     1021 ttctgattat tttagcagtt cttatcgtat tagtattagc aattgtagga gtaggagcaa
     1081 aattatatct ggatctctca cattctattg agaaaactta cgaatcagtg gaacgaaccc
     1141 aaggagaacg aacgaaacca gttgatctgg acaaacaaga atctttctct gttctattga
     1201 tggggattga tactggagat ttaggtcggg tcgatcaagg aagatcagat acgatgatgg
     1261 ttgccacagt cagtcctgaa gacaaacaga caacgatcgt cagcattcct cgagatacgt
     1321 atgtcgacat cgtaggtcgt gacacaaaag acaagatcaa ccacgcttac gcattcggcg
     1381 gagctgccat ggcgatggat accgtacaaa aatatgtaga catcccaatc gatcactatg
     1441 tctctatcaa catgaaaggg ctaaaagaac ttgttgatgc agttggtggt atcgaagtca
     1501 ataatgacct cactttctca caagatggct atgactttac aatcggtaaa attactttga
     1561 acggggatca ggcattatct tattctcgta tgcgttatga agatccaaac ggtgactatg
     1621 gtagacaaga gcgtcaaaga aagatcgtag aaggcgtagc aaaaaaagta ttgagtctaa
     1681 acggtgtcag caactatcaa tccgttttga atgctctaga gtcgaatatg aaaacagaca
     1741 tgagctttga tgaaatgaaa aaaattgctt tagattatcg ggaggcattt ggtacgatca
     1801 agcaagatca gctgcaagga gaaggcttca tgcaagataa tatctcctat caaaaagtca
     1861 gtgatcaaga acttgatcgt gtaaaaaaag aattaaaaga tcaaatgaat ctagagaata
     1921 aataggaaag tggagaaaaa atggaagaga caatcagctt gaaagaaatt tttgaaacat
     1981 tacgcaaaca cttggtaaca atcatcatct caatgtttgc tggcttggcc atctcaggaa
     2041 tcgtgacgtt tttcatcttg acaccaaaat acagttcaca agcacaattg atcgtgacac
     2101 tgccacaatc agacacagca aatgtcaatg atgtcaatac gaacctgcaa atgatcaata
     2161 cgtataaaga tatgatcgtc agtgatttag tactaaatga agtcaaagat cgtttagaaa
     2221 cagaagacaa tgtgaaaatg actgctggac aaatcaaaga tgcgatctcc gtcaatcagt
     2281 cacaaaattc acaaatgttc tcgatccagg caataagcac gaatgcagtg actgctcaac
     2341 agatcgctaa cacaacttca caagttttcc aagaaaacgc caaagacgtg atgaatgtcg
     2401 ataaaatctc gattatttca ggtgcagttg ccaatacgac acctgtttcg ccaaataata
     2461 aattgaatct agtgattggc ttagtgttag gaatgatggt aggcgtaggt ttggcattct
     2521 tattcgaatt acttgatcgg acagtgaaag atgaaaaata tgtgacggat acattaggat
     2581 tcccaatctt aggaacagtt ccagaaatgt cagcgaaaga attgaacgct ggtatccgta
     2641 aaaaaggaac agctcctgca cgaccaaaag ctacaaacga taaaacagcc ccatcacgca
     2701 gaagccgctc aagagtctag gaggaagaaa aatggcacga acacagaaac aaaaagcagc
     2761 acaaactcat gcagtcagtt tgatcacatt aacaaatcca tcttcaccaa tcgccgaaca
     2821 atatcgtacg atccggacga atatccagtt tgcctccgca gctggtcagc aaatcaaaac
     2881 gattgtcgtg acttcatcag gtcctggaga agggaaatcc accactgcag caaatattgc
     2941 agtcgttttt gctaaatcag gtcaacgagt tctactagtc gatgcagatt tacgtaaacc
     3001 cgtcgtctat aagacattcc agctgaacaa tgccagtgga ttaagtacag cacttagttc
     3061 atcaggaagt gtagcagatg tgatccaacg aacgccagtc gagaacttgt caattttgcc
     3121 tagtggacct aagccaccca acccatcaga attgttaagc tcgccaagaa tggatcaggt
     3181 tctagccgaa gcaagacagt tatttgacgt ggtgatattc gatatgccac cagtagtagc
     3241 cgtaacagat gcacagatca tgtcttcaaa aacggatggc actttattgg tcgtacgtga
     3301 aaatatgtca cgtaaagaat ccttgactaa agcaaaagaa ctgctggaga tggtacaagc
     3361 acgagtctta ggtgttgtgt acaacggagc agaacatagc aaagatgctg gttattacta
     3421 cggaaattaa ggaataagaa gagaggagac aagagaaatg atcgatctac attgccatat
     3481 cctaccagga atcgatgacg gggcagaaaa cttagaagca agtattgcaa tggctgaaaa
     3541 agcgattcag caaggcatca cgcacatttt atgcacacca caccacaata atggaaaata
     3601 cagcaatgaa aaatcacaag tcatttctct agtcgcttcg ttacaagccg aattagagaa
     3661 aagacagttg cctttgacgt tattggaagg acaagaagta cgtattaccg gaacgctcat
     3721 cgaagacatc caccgagatg aaattttatt tacagattta gatgatacat atcttctgat
     3781 cgaatttccg acactggaag tgccgttata tgcagaacgg cttttttttg agttgttaga
     3841 actaggaaag acaccagtga tcgtccatcc agaacgtaac gcgcattttc gcgaagaccc
     3901 aaatcatttg cttccgttcc tagatatggg atgtttggcc caactcactg cgccaagtta
     3961 tgttggggta tttggcaaag acatccagaa aacagccaaa acgatggtca aacataatct
     4021 tgtgcagatg gctgcttctg atgcccatgg ggtctctaag cgcacctttt acttaaaaga
     4081 gtgctatgaa cagattgcca aggactttgg taaggaaaaa gtagtacaga tgaaacaagt
     4141 cgcaaaggat ctgatcaacg gggatgaaat cagttatcca atctatgaag aggtaaaaaa
     4201 gaagaagttt ggtttatttt agggggaatg tagggtgttt gtattaacaa gaagaagaaa
     4261 aattgccaca ttggtatttg tggacagctt gttgttgatt gctgcgaata ttgcagcagc
     4321 taaattcatg aagccgtttg tagctatacc aatggacctt attttaatct ctatcggttt
     4381 gtcgattggt ttttatttat tctatggtag tttattcaaa gtattcactc ggatcaatcg
     4441 ctatacaaat ttaagagaaa tcattgcggt ttttggctca ctatctgcaa gtgctgtctc
     4501 aagtatcatc atattgctgt tcataaatcg gcgatacagt ttacgtttag tcatttttgc
     4561 ttacctgctt tcgctattat tgattattgg tagccgattg atctggcgta tctatgtcga
     4621 aacaaaaaat atgagatatc tatccacaga ttctgctaaa aatacattga ttgtcggtgc
     4681 tggcgaaggc gggcgtattt tatacaacag ctttcttgga tccaaaacgg ctcaagatat
     4741 ccacgttgtc gggtttgtag atgatgatcc aaataaaaga aatacgtatc tttcaggaaa
     4801 aaaagtatta ggtgcattaa aagatattcc ggaattgatt gaaaaatatg acattcaaat
     4861 ggtaacgatc gctatccctt ccttatcaag aaaaaaatta cgaagaatct tcgagttagt
     4921 cgaatccgct cgtgtgaaag tcaacacgat gccttctata gaagaacttg cttcaggaaa
     4981 aattagcgta tcaaaattaa aaacaattga cgtagtggat ttactgggaa gagatgaggt
     5041 aaagctggat attgaatcca tcaaagatca gatcacagac aaagtcattc ttgtcaccgg
     5101 cgcaggagga tcgatcggtt cagaaatctg tcggcaaatc attcaattca atcctgcaaa
     5161 gctattgctt ttaggacatg gggagaactc gatctacttg attgaccgtg agttacgcac
     5221 gcatcatcaa aattgtccta cagaaatcgt accgatcatc gcagatatcc aagatcgcga
     5281 gaaaatcaat gagatcatgg aacaatatca tccagatatc gtctaccatg cagctgctca
     5341 taaacatgta ccactgatgg aatataatcc aaaagaagca gtcaaaaaca atattttcgg
     5401 tacaaaaaat gtagcagaag cagccaaagc agcaagagtg aaaaacttcg tcatggtatc
     5461 gacagataaa gctaacaatc ctccaaacgt catgggttca acaaaacgaa tcgctgaaat
     5521 gatcgtgact ggattgaatg aagaaggctg tacgaaattc tcagctgtca gatttgggaa
     5581 tgtccttggt tctcgcggta gtgtgattcc agtattcaga gaacaaattg ctcaaggtgg
     5641 tccaatcact gtaacagact ttcgtatgac ccgatacttc atgacgattc ctgaagcgag
     5701 tcgtttagtt attcaatctg gcgcattagc aaaaggtggc gaaattttta ttctagatat
     5761 gagtgaacca gtgaagattg tagacctagc caaaaacatg atccgattga gcgggtatag
     5821 cgaagacgaa atcgaaatca ttgaaacagg tatccgtcca ggggaaaaac tatatgaaga
     5881 actattgttg gataaggaaa gaaatgatga agcagtttat gagaaaatct ttgtaggaaa
     5941 catcaaaggt tactcaatac aaaaagtgat ggattttgtc aagagcctgc cgcaagacga
     6001 tgaacaactg gctaaagaca ttgtcacttt tgcaaatgct tcaaataagt agggggagta
     6061 aagtgaaagc aattattgta ggagctggtg gatttggcaa agagattgct tttctattac
     6121 aaagtgtctc tagatatgaa ttggttggtt ttgtcgatga ttcgttaaaa atgcaaaatc
     6181 aagaattatt ggaaaaacct atattaggaa caatagactt gttaatagaa ttagaagaag
     6241 aaactgctat ttttctggga attgcttcgc cagatatcaa agagaaaata tatcagaaga
     6301 taaataaaaa taataaattg attttcccta atttagttgc accaagtgca ctagtgggca
     6361 taaatgttca gttaggaatt ggcaatattt tgatgcccta tacgacttac actgctgacg
     6421 tagttttagg aaatttcaat atgatcaata tcggctctac tatcggacat gatactcaaa
     6481 tcggtaatta taattcagtt tttccaagtg taaatatttc aggacatgtt atattaggag
     6541 ataaaaatga aattggagta ggtactaaaa taatacaaaa tctaagtatt ggaaatacga
     6601 atattatagg tgcaggaagt gtagtaataa aaaatattaa taacaatact aaaaatgttg
     6661 gcgtacctac caaagtaata gaaagatggg attaagttgg aaaataaacg aatattatta
     6721 gcatctcctc atatgagtga ggaaggatat gaacaagagt atgtaaaaga agcatttgat
     6781 acaaattggg tggctccttt aggtgcgaat gtgaatggat ttgagaaaga attagcagaa
     6841 tacgtgggag ctagtcatgc tgccgcatta tcttctggaa cagcagctat acatttagct
     6901 ttgaaagcgg ctggggtcgg taaagatgac atagtgattt gtcagtcctt gacgttctca
     6961 gctactgcta atccaattct ctatcaggga gctatacctg tatttgttga tagtgattac
     7021 gaaacttgga acatgaaacc tgaacatttg cgaacagcgc tagaaaaata tcctaacaca
     7081 aaggctgtat tagtagtaca tttgtacggt ttatcagcta acttagacga aattactaaa
     7141 atatgcaaag aatatgatgt tactctaatt gaagatgctg cagaatcatt aggaacaaca
     7201 tataaaggaa aatatactgg tacattcggc gactatggaa tttattcttt taatggaaat
     7261 aagataatca caacttcagg tggcgggatg cttgtgtcta atgaaaaaga aaaggtggaa
     7321 aaagtgagat tttgggctac acaatctcgt gagaaggcac gccattatca acataatgaa
     7381 attggctata attatcgaat gagcaatatc gttgcaggta tcggtcgagg acaattgaaa
     7441 gttttaaatg aaagaattga gaaaaaacga tatattcata atttttataa aaaagaattg
     7501 tctaaattgt caaatataca attcatgcca gaaaataatt ggaatgaacc gaactattgg
     7561 ttaagcgtca taacaataaa ggggataaat acgaatcatc ttattgatga gttagataaa
     7621 aataacatag aagcccgccc agtatggaaa cctatgcatt tacaacctgt tttcaaaaat
     7681 tatgattata ttggcgatga tgtagcagaa actttatttg acttaggagt atgtttacca
     7741 tcagatacaa agatgacaga tgaagattta aaatatgtag tatctacgat taaaagagtg
     7801 atcaatgatg cgtaaaaaaa ctttctatca gctttttggg aaaagaattt tagatatcct
     7861 attgtcaggc attgctctca tcgttttatc gccgataatc ttaattgtag gttttttagt
     7921 aagaattaaa cttggttctc caattatctt caaacaagaa cgaccaggaa aaagtgaaaa
     7981 aattttttcc atgtataaat ttcgtacaat gactgacgaa agagaccaca atggagaata
     8041 tttacctgat gaaattcgac taactaaatt tgggaaaata cttagagcaa caagtctaga
     8101 tgagctacca gaactttgga atattttgaa gggtgatatg tcgatcgtag gacctcgtcc
     8161 attattagta gagtatttgc cactatatag tgagaagcaa cggaaaagac acaacgtaag
     8221 accaggatta actggactag ctcaggtcaa tggaagaaat gcgattagtt gggaagaaaa
     8281 gtttgattta gatgtttatt atgttgataa aattagtttc tttaatgatc ttattattat
     8341 aattcagaca tgtaaaaaag taatcaagaa agagaatatt aatacaatca ataacaatat
     8401 accagaaaaa tttaaaggga gttaaatata gtatgccatt aaatatttta tttcttacgc
     8461 ttttagattt tcaaagttta gaagaaagca atatctatac tgatttgatg tctgaatttg
     8521 taaaaaataa tcacaatgtg tccattatat ctccaacaga aagaaaaaaa agaataaaaa
     8581 ctcatttgat cgagataaat gataaatgtg aaatcttaaa attaagaata ggaaatattc
     8641 aaaagactaa tttaattgaa aaaggttttt ctactataca aatagagaga aaatttatat
     8701 caggcattaa tcaatatttt tcaaacaaaa aatttgatct ggttatttat tctactccgc
     8761 cagtaacttt tgctaaagtg gtgaagttta taaaaaaaag ggataattgt aaaagttatc
     8821 ttttactaaa agacatattt ccacaaaatg cagttgattt aaatcaactc tctaaaaata
     8881 cttatatata taagtatttt agaaaaaaag aagtttctct atatgaagta tctgattata
     8941 taggaacaat gtcagaacaa aattctcaat atataagaaa acataataaa attgatgagc
     9001 aaattttaga agtaaatcct aattcaatta gaataaacga aaataaaatt ggatcccctc
     9061 tagacaaaga ggaaataaga aatttatata aaattcctaa agaaaaaact ataatgttat
     9121 atggaggaaa cctagggaaa cctcaaggta tcgactttct tttaaatatt ataagtagcc
     9181 tagaaaaaga taacaaaatt ttattgctga ttattgggtc aggaacagaa tatgggcgta
     9241 taagaaaaca tataaatact aataatataa aaaatacaat tctaattgat agtttatcta
     9301 aaaacgaata taaaaaagtt gaatccattg cggacatagg attggtattt ctagataaaa
     9361 aatttactat ccccaatata ccttctagaa tattaggata tatggagtca aaaaaaccta
     9421 ttttagctgc tgtagacaag catactgatt taaatgaaat gataaagact ggaaaatttg
     9481 ggtattgttc tgaacatgga gatttaaacg catttctaag taatatggag cgattaaagg
     9541 atgaaaaaat aagaaacgaa ttaggtaaaa atgcttttat ttatttgaaa aataattttg
     9601 atgtaaaaag aagctattct ataattatga atcattttga attataaaaa ggagcaattt
     9661 aaatgttcaa agataaaatt ttattaatca caggcggcac gggttctttt gggaacgcag
     9721 tgatgaaacg ctttttagat tcagatatca aagaaatacg tatcttttct cgtgacgaga
     9781 aaaaacaaga cgatatgcgg aaaaaataca ataatgacaa attgaagttt tacattggag
     9841 atgttcggga catcaacagt gtcaaaaatg cgatgtacgg cgttgattat gtcttccatg
     9901 cagcagcact taaacaagta ccttcttgtg agtttttccc tatggaagct gtgaaaacaa
     9961 atatcatggg aacagataat gtcgtaactg cagcaatcga aatggggatt caaaaagtta
    10021 tttgcttgtc cactgataaa gctgcttatc caatcaatgc tatggggatt tctaaggcta
    10081 tgatggaaaa agtatttgtt gctaaagcaa gaaccgttac tgacgaacgc acgatgattt
    10141 gtggcacacg ttatggaaat gtaatggctt ctagaggatc agttatacca ttattcattg
    10201 accaaataaa aaatggtaaa ccattaacag tgacagatcc tggcatgaca agatttttga
    10261 tgagtttgga agaagctgta gagttagttg tttttgcctt ccaaaacgca aaagcagggg
    10321 atatcatggt tcaaaaatca ccagcttcta ctgttggcga cctagcgcaa gccatcaaag
    10381 aattattcca tgcagacaat gagattcata tcatcggaac gcgacatggc gaaaagcgtt
    10441 atgaaacact actgacaaaa gaagaatact tagttgctca agatatgggt gatttctatc
    10501 gtgttcctgc agatacacgt gatttgaatt atgacaagta tttcgttgaa ggaagcaaag
    10561 agttgacaca agaaggagaa tacaattcag ataatacaca acgtttatct gtagaacaaa
    10621 tcaaagaaag acttcttgaa ctggactatg taagaaaaga attgaaagag tggcaaaatc
    10681 aatgaatatt ttagtaactg ggtcaaatgg ttttattgga aaaaatttag ttgctgaatt
    10741 aaaaaatgaa ggattcacag acattcttag ttttgataga gacacgcctg tacatttatt
    10801 ggatgaatat tgcgaaaaag cagactttgt ttttcattta gctggagtga acagaccaaa
    10861 agacgatgct gaattcatga caggaaattt tggttttact tcagaattgc tagaaaaact
    10921 aaaagagcat ggcaatacat gtcctgttat gatttcctct tctatccaag cagaacttaa
    10981 taattcttat ggcaagagca aaaaagccgg agaagatcta ttaaagaaat accaaaaaga
    11041 aacaggagca cctgtatata tttatcgctt ctcaaactta tacggtaagt ggtcgcaacc
    11101 aaattataat acagttgtag ccacattttg ttacaaaatc gcaagggacg aagaagttac
    11161 ggtaaatgat ccgtctgcta tcatagagct ttgttatatc gatgatgtcg tgaaggaact
    11221 actccattgt ctgaacggac aaccttatac agaaaatggg tattgtgctg ttcgtgaaac
    11281 ggatgaagta acggtcggcg aattaaaaga actaattaca tctttcaaaa atagtcgaac
    11341 gaatttgagt gttcctaatc ttgcgagaag agtagaaaaa aatctgtaca gtacgtactt
    11401 aagcttctta ccagaagacg aattttctta ttttctaaaa atgaacgtag acaatcgagg
    11461 atcgtttact gaatttttaa gaacaaaaga ccgcggtcaa gtatctgtca atatttccaa
    11521 accgggaatc actaaaggaa accattggca tcatacaaaa aatgaaaaat tcttagtagt
    11581 aagcggagaa ggtgtaatcc gttttagaaa agtagactca aatgaaataa tcgagtattt
    11641 tgtttcagga gaaaaattag aagtagttga tattccagta ggttatacgc acaatatcga
    11701 gaatctggga tcaacggata tggtgacagt gatgtgggta aatgagccat ttgatccaga
    11761 acatcctgat acttattatg tggaggtcta atcaatgaaa aaattaaaag tgatgacagt
    11821 agtaggaacc cgaccagaaa tcattcgttt gtctgctgtt atcaaccgtt tggatcgatc
    11881 agaagcaatc gatcatattt tagtacacac tggccaaaac tatgactatg agctaaatga
    11941 agtattcttc gaagacttca agctaaagaa acccgattat tttctaaatg cagcaactgg
    12001 tacagcgatt gaaacagttg gaaatatttt gatcaagatc gatccaattt tggaagaagt
    12061 aaaaccagat gcattcttag tattagggga tacaaatagc tgtttatgtg caattgcagc
    12121 caaacgtcgc catattccta tttttcatat ggaggcagga aatcgctgtt tcgaccaacg
    12181 agtaccggaa gaaacaaatc gtaaaattgt tgatcataca gctgatatta atctaaccta
    12241 tagcgatatc gcgagagaat atctcttacg tgaaggatta ccagctgatc gtgtaatcaa
    12301 aacaggaagt ccgatgtttg aagttctcaa ttcaagaaaa gatgacatcc aacattccga
    12361 tgtccttaat cgcttagggt tgaaagaagg acaatatttt gttgtttctg ctcaccgcga
    12421 agaaaatatc agctcagaaa caaatttctt acacttggta gatagcttga atacaatcgc
    12481 tgaaacttac caattgccaa tcatcatcag tacgcatccg cgaacaatga agatgatcga
    12541 agcaaaagaa atcaaattcc atgagctgat ccaaacaatg aaaccaatgg ggttcaatga
    12601 ttacaataaa ttgcaaatca atgcaaaagc tgtactaagc gacagcggaa cgattagcga
    12661 agaatcttcc attttgaatt tcaaagcatt aaacatccgt caagcacatg aacgaccaga
    12721 agcaatggaa gaagcaagtg tcatgatggt aggattagaa aaagaaagaa tcatgcaagg
    12781 actggaagta ttggaaacgc agcaaaaaga tactttgcga cacgtcgcag attattcaat
    12841 gccaaatgtt tcggaaaaag tattgcgtat catcctttcg tatacagact atgtgaatag
    12901 agtggtgtgg ggaagatgag aattgttgta aatgatatcg ctgcttcaga aggtggggca
    12961 atgtcagtat tgagagactt acattctgat ataataagta gtggtgatga aaatgaatgg
    13021 atttttttac taggtgaaaa attcatagaa gaaagtgaca atataaaggt actaactttt
    13081 cctaaaataa aaactagctg gattaaaaga ttggattttg attttattag tggaaaaaaa
    13141 attatcaata aattacagcc tgatatctat ttatcattac aaaatacagc tactattggt
    13201 gtaaaagctc ctcaatatgt ttttttacat caaccactac catatcaaaa ggaaaaaaat
    13261 ttttcgttta taaaaaaaaa agagtttaaa atggctacct atcaacatgt tatcggaaag
    13321 atatattcta tattgtttaa aaaaactaaa gctactatta tagtgcaaac tcaatggatg
    13381 aaggaaaatg tttccagtat attaaacaat aaaatactta aggtagatac aaaaataaat
    13441 attgatgaaa atatcatcaa taaacaaata aatgaaaata aaagaaatat gaaacatttc
    13501 ttttatccag catcaaatat tccgtataaa aaccatgatt ttcttataga gagtttttca
    13561 aagtgtaaag ataaagaatt aagacttttt ttaactttat ctagagatga aattgatatt
    13621 aaagatgata gaattatatt attaggtaag gtacctagag agaaagtttt tgacatatat
    13681 agtaaatcaa atttaatatt tccctcatca attgaaactt taggcttgcc attattagaa
    13741 gcacaatgtt ttatgagtcc aattctagct ttagaaacag aattctctaa agaagcattg
    13801 tccggttacc cccataagta tctatttaaa ctagataatt tggacaattt aagtcattta
    13861 atagagaaaa taagtacaag tagtatagta tttaacgttg agaacgtcat ttataagaaa
    13921 gaaaatagag tttctatctt agatatatta aaaggagaga atatttattg aagaaatttg
    13981 tagtagcaca tccagctcaa caacactctt ataaaatcgc tgaagcatta attgaaaaag
    14041 aaatgttatt caaatatatt acttcagttt atttaaaaaa aggaagtata ttgaataaac
    14101 tattaaatat tattccagga gaaaataagt tacgagcgaa aggtcgtcat agtgataaaa
    14161 tatgtgatga ggaggtatta ttaattaatg aattttcagg gttgctactt cttattttac
    14221 agagggtgaa atttttaaaa catttataca aaaaatattg ggatttccat aataaaaagt
    14281 ttaataaaaa attgattaaa tatataaatt cgaatattga tgagatatcg ggagtaattg
    14341 tttttgatca ggtatccttt gatttcttta aaaaaaagat taacattcca ataatactag
    14401 acatgtctgc acctagtgtt gtatttatga aaagtcaatt tgaaaaatat ggaatatcaa
    14461 cattgaaata tgacgcactc ttaaaaaaga ataatttcga aatcaatagt gcagattttt
    14521 tcattggggc ttcttcaata acagaaagat ccttattaaa tatgggaatt gaaaaaaaac
    14581 aaataaaaat tttcccttat ggagtgaata tagttaagga aaatgataaa ttaatgaatc
    14641 taagaacaag aaaaaaaaga ttaaaatttt tatttgtggg aagagtaacc ttagaaaaag
    14701 gaattggggt tttagatagt ttttttgaaa aatttggaat tgaggatgca tggacatttg
    14761 ctggcgatta ttcagattct aaaaaatttt atgaaaaata tgaagataag gtacaatttg
    14821 tgggacatgt atcaaaaata aaaatgtttg aactgtacag ggataatgat gttttagtat
    14881 ttccttcttt agcggatggt tttggtttag ctgttattga agcattaagc aatggaatgt
    14941 atgttatatg ttcagattct acaggtgcta aagatattat tatggaaggc aaaaatggag
    15001 atgtgtttct tacaggagat gaaaatgatt taaataataa aatacaatat tataagaaaa
    15061 atataaataa gattgatcgt ttaaaaatta aagaaactgt aaatgattta acatgggaac
    15121 gttataacaa aaacattata aaatcattag aatattttac cgtaggtatg aacaatgaat
    15181 aaattattaa tgatgttaaa ctttttttat ctctacgtat acatgattga cgcatacggt
    15241 ataataaata atgatttata tctaagaatg gttttgatta tcttatttat aatgataggt
    15301 ttaataaatt gtataaaaga catcatgaat aaaatataca gtaaagataa tctttttttt
    15361 acgttgtatt taatctattt tctaagtacg caatatatgc tttggaatat tgatatatct
    15421 cagctaataa tgattatcta tactccaatg atgtttatag ctttttctag gtttcatatc
    15481 gacagatact tttatgagaa atttaccttt agttatattt tgcttatgac ttttacttac
    15541 ttttatactc gtttttttct taatataaat aatggtttag tgataaattc ttgttattat
    15601 ttgttattaa ctgttcctat agtttatttg tttgttaatc cttatagaaa aaatattagc
    15661 tttttattaa ttagtatcat aattttattt tcaatgaaaa gaacagcatt gataacatta
    15721 gcactaatta tttttattga gttaatttta aatgttttga ataaaaaaag aattagttta
    15781 agaagattag catttcttat accgtttagc ctttttattg cagcattgct aattaaaata
    15841 aacaaaataa tatctagttt gtatgataat aatatattta tgcgatttta ttctggagat
    15901 acaataggcg aatctagagg tgacatttta aaattattat tagaaaaaat atgtacgaac
    15961 tcaaatatat attttatttt attaggacaa gggatcaact caactatata ttatacaggt
    16021 ggtttaacag cccataacga ctttctggag gttttatttg attttggcat ttttggtcta
    16081 ttattatata ttatgattta tataattttc ataaaaaaaa taaggattat tacaaacgta
    16141 aaagacaagc gatcattgtt atatactatg gtcatcttaa tgctagtttc cgtaagtagt
    16201 catctaatat ttattcctag ttatgtaagt ttattaataa tgaattataa ttatctgaaa
    16261 aataaaaatt acttttcaaa ggagaactaa tgaagaaaat tggtatttta acatttcata
    16321 gagctgaaaa ttatggagct gtattgcaga catatgcttt acaggaatat attagtaaaa
    16381 tgagtagtaa ttacgaagta gaggtaattg attataggtc accatttata gaaaaatact
    16441 ataaattact aagttttaaa gcatttacta atattaaaag tcttaaaaga atagtatacg
    16501 ttttattata taatggtacg ttaaaatcaa aaagaaaaat ttttgataat tttattgata
    16561 aatttattcg tgttagctta tcagagtatg atgataaaac aataagaggc ttggatgata
    16621 aatatgatct ttttatctgt ggaagtgatc aaatttggag tccatactcg gctggtttcg
    16681 atcccatata ttttttagat tttgtaaaaa aaaggaataa aaaaaatgct tatgctgcta
    16741 gttttggagt agaaattgtc ccggaagaaa ctataaaaga gtatagtcga cgtttaaaaa
    16801 actttaatgt aatatcagtc cgtgaagaga ttggaaaaaa aattattaaa aacttgattg
    16861 aagaagatgt acctgtatca cttgatccaa cactattact tagtaaaact gagtgggatt
    16921 taatcattga gaaaaaaaga aaaatttcag agaaatattt attgatatac ttaattacag
    16981 aaacaagaga aattattgag ttagcaaaaa aagtagcaag agagaaaaaa ttaaagttag
    17041 tatatataaa cgataatatc tacaaaacaa agggaatata taattataat aattgttcac
    17101 ctagtgattg gctcaattta ttttataatg cagattatat tgtaacaaat tcatttcatg
    17161 gaattgcttt ttctataaat tttgaaaaag aattttctgt acagtattta cctgaaccag
    17221 cgaaggttaa ttccagaatc aataatattt tagataatta tagtttgaaa tggagaatta
    17281 actatgatgg taatgagaaa attaattata gtaaagtaaa caaaatactt gtagaagata
    17341 gagttgcctc ccaacaaata ctaaaagata ttatggaata ttgaggtggt attatgaagc
    17401 caaaagtatc tataattatt cctgcatata atgctgagta tactatttcg agagctctcg
    17461 aaagtgcact taaacaaact tattctaata aagaaataat agtcattgat gatgggtcta
    17521 aagatagaac attagcaatc tgtaaagagt atcaattaaa taatcatgaa atatttatat
    17581 tctcacaaaa aaattcaggt gtatcagtag ctagaaattt gggaataaga aaagctgatg
    17641 gagaatttat acaattttta gatgctgacg atgctatgga acctaatatg actgagaaac
    17701 ttattgaagt tcagcgcaat aatgattctg actatgtaat ttgtgggtta actattataa
    17761 aagataataa tatagtgaga accccacatc taaaggaaag aaatatcaat tttattaatt
    17821 ttgaagatta taaatatatc tatcctttgc ttgctgcacc atgcaataaa ctttttaaaa
    17881 aggagcttat tgtactattt gaaaaaaatt ttagcaatgg agaagacttg ttgttcaata
    17941 cggaatattt aacaaaaata aaaaaaattt caatcattga agactgttta tataaggtat
    18001 acttagataa tgaaaattct ttaaacaaaa aaatgagtat atctactgta aaatcgttat
    18061 tattaatcaa tgaatatgaa aaagaaataa tgaataaatt atttgataat gataatgttc
    18121 ttgaatttaa tgtttttaat aaatttaaaa tcatacagac gtctttacaa aaaatagtta
    18181 acaatgaaga aaaagatgat gttatagaat tgataaataa tttaaggtca gatagtcaat
    18241 ttctaacgat attagaaaaa acaaacatga aaaacatgaa aagcaattta atcaaatttt
    18301 gttttagtta ttgtgatagc caaacaattt atcagttgtt ttttttaatt agtaaagtac
    18361 gaggttactt tcaatgaaag atagagtaaa aaatgtaatc tataatgcag tatatcaagt
    18421 atttataata gttattccta tacttactat gccatatgta tcaagaaagt taggggcaga
    18481 aaacttaggt ataaatacat atactcaatc cctagtattg cttttaggat tatttatcaa
    18541 ttctagcatt ctacaaattg gcgcaagaga aatcgctaga caggatagaa ttagtagaac
    18601 aaaaacattt attacacttt ggttattaca agtactatgt gggttggtta ttatcatagt
    18661 gtatatatta atcattatgg gtagcaaata ctattattat tatttactac aattaccttt
    18721 tttaattagt tatatatttg atatatcttg gttctttgtc ggattagaaa aaataaaaaa
    18781 tgttgtattt agaaatatgt tcgtaaaggt atgtacaatt tttttgattt ttctattgat
    18841 aaaaaataaa aatgatcttt ctatatacat cattattatt tctttgggat cgttagttgg
    18901 taattttata tttttcttct ccttaaaaaa atatctcgat ttcgacaaga ttgtcggttt
    18961 agatttatct aacttaaggc catttattaa ttcaattgta atattgcttg ttcctcaagt
    19021 ggctattcag ctatatcagt atataataaa atttagtatt gaaaattcta ctacgactac
    19081 tgagttatct tattttgatc aatctcaaaa aattgctaga ataatcattc aattagttag
    19141 ttcaattagt attgtattaa tgccagtcat tgcaaatcta gataaaaata aatccagaaa
    19201 tgctctgaat aaaattattc agttgtcggt aaattattca ttaagtatag cggtgatatt
    19261 tacaattttg cttgtaataa tttcacccac tttcgttcca attttttttg gtcctgaatt
    19321 taaagaaatg atacctcata tgataatctc atcttttatt ataattccaa tttgtgtagg
    19381 tggagtcttt tctaaccagc tcattttagg gatggggttg tataaatatt atgctacacc
    19441 atatcttata ggtggaagta taggaatttt aatttgctta atttataaag acaaaatgag
    19501 tggattaatt ggatgtatag tactagttat agtagaatca cttgtttgct tttttagaat
    19561 aatatttgca aataaaataa tagatataaa agaaaattta caagggacaa taaaggaatt
    19621 cttcttatta ttcttattat tagctgaagt atacattgta aataccatat ttacgccaag
    19681 cccatcagcg ttattaaatt tattttatat gatgatcata attatacaat actttgtttt
    19741 aagtactata ataaaaacag agactgcgaa agatttatca aaacggttaa aatcattttt
    19801 aattaaataa cactttacca aactaaatct ggaatagaag tttat
//
LOCUS       DACEDO010000053        19658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEDO010000053 DACEDO010000000
VERSION     DACEDO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.053"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            149..1504
                     /locus_tag="IWO52_001984"
     CDS             149..1504
                     /locus_tag="IWO52_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0356246.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTISSTESSTTSSSTSEITSS
                     KVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVE
                     NFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNF
                     VIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSK
                     TYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSI
                     TSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALY
                     AHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFG
                     D"
     gene            1853..2485
                     /locus_tag="IWO52_001985"
     CDS             1853..2485
                     /locus_tag="IWO52_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0356247.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3073..4512
                     /locus_tag="IWO52_001986"
     CDS             3073..4512
                     /locus_tag="IWO52_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0356248.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4807..5025
                     /locus_tag="IWO52_001987"
                     /pseudo
     CDS             4807..5025
                     /locus_tag="IWO52_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5274..5489
                     /locus_tag="IWO52_001988"
     CDS             5274..5489
                     /locus_tag="IWO52_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356249.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5706..5962)
                     /locus_tag="IWO52_001989"
                     /pseudo
     CDS             complement(5706..5962)
                     /locus_tag="IWO52_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6149..7060)
                     /locus_tag="IWO52_001990"
     CDS             complement(6149..7060)
                     /locus_tag="IWO52_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0356250.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7079..8083)
                     /locus_tag="IWO52_001991"
     CDS             complement(7079..8083)
                     /locus_tag="IWO52_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0356251.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8080..10203)
                     /locus_tag="IWO52_001992"
     CDS             complement(8080..10203)
                     /locus_tag="IWO52_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0356252.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10208..12655)
                     /locus_tag="IWO52_001993"
     CDS             complement(10208..12655)
                     /locus_tag="IWO52_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0356253.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12642..13031)
                     /locus_tag="IWO52_001994"
     CDS             complement(12642..13031)
                     /locus_tag="IWO52_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0356254.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13091..13594)
                     /locus_tag="IWO52_001995"
     CDS             complement(13091..13594)
                     /locus_tag="IWO52_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0356255.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13607..13831)
                     /locus_tag="IWO52_001996"
     CDS             complement(13607..13831)
                     /locus_tag="IWO52_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356256.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13935..15845)
                     /gene="ltrA"
                     /locus_tag="IWO52_001997"
     CDS             complement(13935..15845)
                     /gene="ltrA"
                     /locus_tag="IWO52_001997"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0356257.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16591..16710)
                     /locus_tag="IWO52_001998"
     CDS             complement(16591..16710)
                     /locus_tag="IWO52_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0356258.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(16725..17909)
                     /locus_tag="IWO52_001999"
     CDS             complement(16725..17909)
                     /locus_tag="IWO52_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0356259.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18168..18458)
                     /locus_tag="IWO52_002000"
     CDS             complement(18168..18458)
                     /locus_tag="IWO52_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356260.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18448..19590)
                     /gene="dcm"
                     /locus_tag="IWO52_002001"
                     /pseudo
     CDS             complement(18448..19590)
                     /gene="dcm"
                     /locus_tag="IWO52_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT         5804 a         4009 c         3486 g         6359 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcgt caagtgaaag tagtacgatt tcaagtaccg aatcttcaac
      421 aacttcaagt agtacaagtg aaataacttc ttcgaaagtg acaaaaccaa accatataga
      481 atcttcaaaa aaggaagcgc caaaaacgga aggacaaaca aaaacttttt cgacaccaca
      541 gcaaatagaa tcaacagaat ctttccaatc acaaccaata ctatctggta atgaagaaat
      601 tcattttgac aaaaatgaat ctgtagaaaa ttttattatg aaaattggtg agtctgcgag
      661 aaaaattggt cagaaatatg atttatatgc ttctgttatg attgcacaag cgatcttaga
      721 atctgccagt ggtcaaagcc agttagctca ggcaccgaat tacaatttat ttgggataaa
      781 aggaacttat aatggaaact ttgtcattat ggtaacaaac gaagatttgg gtaatggaac
      841 gctttatacg acccaatcaa aatttagagt gtatgaaaac tatgaggaaa gctttgaaga
      901 ttatgccaaa ttattaacta agggaattag tggaaataaa gatttttatg caggagcatt
      961 aaaagctaat agtaaaacat atcgagaagc aactaaattc cttacaggac ggtatgcaac
     1021 agatacacaa tactatttga agctaaatga attaattaaa acctacgatt taaccaacta
     1081 tgataaagaa gttaaaggtc cacaaatgag taagactgga taccaagtgc ctattcataa
     1141 ttattcaata accagtttgt ttggaaatcg tggaaatgaa tttcaccgag gacttgatat
     1201 ggcagctaca cagggtgagc cgattcatgc agttaaagct ggtaaggttg ttaaagcaga
     1261 gtttcactac tcttggggaa attacgtggt gatacaacac gaagatggat caaccgcgtt
     1321 atatgctcac cagcaacaat acctagtaaa aactggagat aatgttttgc aaggacaaat
     1381 aattggctat gtaggaagta ctgggaatag tactggccca caccttcatt tggaaatttg
     1441 caaagatagt agtctttctc agagcatgtt agttgatccg aaaaccttat tattcggaga
     1501 ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg gttggattct tagacctttg
     1561 atcaggatat tgagactgga attgatatct cgattacgat gggtttgaca aataaaacaa
     1621 gtccattatc gaatatcgag tgtattattg ccgtctttat gctcatattt tgagcaggtt
     1681 tcactagatg aaaacaattt atctatcttg aggatttcgt gtacgtatcg ttcagtcttg
     1741 tattgtaaat tagtcataaa atcagactaa gaaaaattgg aaatattttt ttctgattta
     1801 ttaaaagtta attgaaatta tatggtactt ctttcttggg agggtaggtg cagtgaagga
     1861 aagatatggt caccttactg ttgttgagga gattactcat aatggatata aaaaatatcg
     1921 ttgcctatgt gactgtggga atgaaacgat tgtatatcga tcaaacttac taacaggtag
     1981 aaccaaaagt tgtggatgtg gaaattataa aaataggcat aaatataaaa atttgacaaa
     2041 taaaactttt ggtaagttag ttgcaatcaa gccaactaaa ctgcgaagtg aaggaactgt
     2101 tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta gtatcggcaa agcgacttct
     2161 aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa aagcatcaca ttgaaaataa
     2221 aaaattcgga ttacttactg caaagtatcc tttggacaat aactgttcat ggaaaacaaa
     2281 atgggtttgt cattgtgagt gtggcagtat gtgtgagata agttatagta acttattttt
     2341 tggacatact caaagttgtg gatgtttaaa aaggcaagaa acaaggacat gtgttgaagg
     2401 gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat aatacaagtg gtgttaaggg
     2461 agtttatcgt tctaaagaga aatagattgc gtatataact ttaaaaaaaa agaggtacta
     2521 tttaggatcg tatgattcta ttaagagtgc agctcaagct agaaaagatg ctgaaaataa
     2581 attgtttcaa cctttgattg acaaatatcg aggtaatcat taattattgt ggataataat
     2641 taacagtgat atataaacat ctataatata ttattcatac tattgatatt tagtataatc
     2701 ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa attggaaaac aaataggtgc
     2761 aacaccataa atatgaaaaa attgggaaaa agtagaatgg aaatttgctt acgtctcacg
     2821 agatagctca cactattaat ctgaagaaca atgaagtcga cttttagaaa ttgctagaag
     2881 aaagtaaagc gaattatcac tagttactat agagtaagtt caaataaagc atctccaact
     2941 tacatggtta catgaggata tcaaatcgaa attactcaag cttcctatgg ctagagtcgc
     3001 gagaagttgt tgggcagtag acataggtta tattgttctt agctacacga tcattagaaa
     3061 ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag tccaaaaaaa tcgctggtgg
     3121 attttgattg ctatatctat gtttaccttc atgtccacat tggatggcag tattgtcaat
     3181 attgcactac ctaccatctc agatgatatg ggcataccaa tgaatcaagc ggaatgggta
     3241 gtgtccgttt atctgatgat tgtctgttct tgtttgttgc tgtttggtaa aattggcgat
     3301 agccgtggga aaatcaaagt atttcggatt ggtacgctga tctttgtctt gggatcgctg
     3361 ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg cacgggtggt acaagccatt
     3421 ggtgctagta tgactatggc tacaaatgcc ggaatcatca cggaagtctt tcccatacat
     3481 gaacgtggtc gggcattggg agcaataggt gcgtttgttt cattaggctc gattgctgga
     3541 ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg cgtacatttt ttggatcaat
     3601 gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat tcttaccaaa agatatcacc
     3661 atgagcggcc atacaatcga tggagggggc tttgctgcca ttgcggctac gattatcagc
     3721 ttttttggag caatcttttt ggggcaagaa agtggctttt tccatccagt cccccttgta
     3781 ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa aagttgaaaa gaaagcagcc
     3841 tctccattga tttctttcgg tatttttaag aacaagattt ttaccatgag cttgattaca
     3901 gccttgttga ttttttcttc caacttcttt gtcaatgtag tgattccttt ttatctgcaa
     3961 aatgctcgcg ggttgagtcc tagctatgct gggctgttga tgatggtctt tcctttgatg
     4021 atggtcgtcg gggcgccttt aagcggctac ctgaccgacc gggttggccc ggagattttg
     4081 gtattagtag gcttgggctt attgtcggta acccaggtca tgtatatgtt tttgtcagtg
     4141 gactctcctt tgtggctata cattgtcgct actgccatca tgggtgccgg caatgccttg
     4201 ttccagtccc ccaataatac gatggttatg agctcggtca ctatcgaaaa cttgggactg
     4261 gcaggctcga tgaattcctt tgcccgaaat ttggggatgg tcagcgggat ttcgctttct
     4321 acgacggtct tatatcaagc gatgtctgct aagatgggcc agcaagtaac taactatatc
     4381 gcaaatcggc cggatgtctt tttatatgga atgaagatta cctttttagg cagctttgtt
     4441 ttgtgttttg ttgccttttt cctgactatc tggcgtatca aaaaacaagg aggtattcag
     4501 aaaaatgtct gaaaaaacca ctgttgaaga atttttggag gcctgcgtat gggtgagtgg
     4561 gatttccatg gatgatggaa gatgaagtga acttctaaaa gccgaaaaac tcaagtatta
     4621 tcttcaaaat actagtgctc aatgagatgc ttgatttgat cacaagaaga ctgcgaaagc
     4681 cgctggcaac cgcaagctag gtatcccacc gatggactac gttttagagg ctactggtta
     4741 tgaatacgga gccaacgctc tcatctgcat aagaatacat cacccagatt atgtaaagat
     4801 tttcgatact tctgtcaaaa aatcattaag atagataaat gatttccgtc tagtcaaatt
     4861 ggttcagaat gtagacacac agatggtaag aagtcactca caattcgaga atggacttgt
     4921 cctatttgcc gtatacatca taatcgaaac gtcaacgata gtatcaatat tttgaccaag
     4981 ggctatgaat acaagcattg ccttagaaaa catcaagaac cttaggaaca acggggatag
     5041 cttggttaat aagagatacc gctgtttagg cttgcaagta tactctttta tcaagaattc
     5101 cgtggcttta gtcatgagag gcttaatact atgaaaattt tctttagcat ttttgattta
     5161 ggttcctttt ctaattataa taagatgacc actacagtaa cctactgaaa tatataaaca
     5221 gccactgtag taccattttc aaagtagtct atgagaggag aatcttcatt cttatgaacg
     5281 ctggaattgc ggatatgaat ttaataaaaa aaacccttaa tgattttact tccaattcaa
     5341 tatcaaaagg tactggtatc aatttaagca caataaaaaa gctgaaaagt ggtgaaagat
     5401 cagtggaaaa gctgaatctt ttagatgcta taaaaataac ggaatttgct atgaaaaatg
     5461 gaaaagctga gattgaaata tggagataat tatcgtctag tgaattactt gccacttagc
     5521 tgaatttaac gatccttggt gcttgaaggg ggagattctt gggaatagag catacttgca
     5581 agcctatgtc tttactaaca acgatatctc ttattaacca agctatcccc gtagttctaa
     5641 ggcttttact tttatctaag caataatcgt attcaaagga aatttttgac ccttagaaag
     5701 tctcatttta tcagacgtga gcttaggcat tgctgaattt tgttaattcg gttttaggtt
     5761 gtgctttgag cataatatga aagtattctt tatcacgatt tcattcatct aaggtaatgt
     5821 gatgtgattc tgaaattctt tcaaaggcag tttttgcata agctgacatt acatcatcaa
     5881 tgacatgttt acaatgtttc actactaaaa taagggggta accaagaaga aaaactgaat
     5941 gattatttgt atctaattcc atggtatatc aaccatttcc tatatagact gattatgaca
     6001 tggattaaaa taaaaaggca ctatgtctta attgtcggtt ttcgagttac cgaatggcat
     6061 tcatcaccca cctatagagg atgggcgact tctgcctgaa ataagttaca ctacttataa
     6121 cttctaataa aattagattg agtcattatt atttaacatc aacgattttc cagtttccat
     6181 ttttttgtaa tactaatttg tactgaaagt agctagtggt ttttgataca ttatcaaggt
     6241 attttatctt tgtactcact agaaaatttt ttccgttttg tagataaact ggattctcta
     6301 actccgaaaa ctttaagtca atattgattg gtcgcatcgc accattttca acataatact
     6361 ccaattcttt ttcagaagct gatggataaa gtttaaaaaa tgtttctaaa aaatcggtga
     6421 tttcttccgt tgttttggca tcaatttctg aatcgttttc tacttgtttt gcttcatatt
     6481 ttgccttgtc tggctttcca atcattgttg gcaaactagt tactaaatga ttcctgtttt
     6541 catcttcaaa gattgtcact cgatatgcag aagaaattga ttgcgtcttt tttccctctg
     6601 taatatgttg ctccactaaa aatgaaacga caaattcttc cgagtgtttt tcaacatcta
     6661 gaatttttac agattgaact tcggaactcg tgggaatatc tgctcgaacc atatcttgga
     6721 ttaaagcgag accgttttct gtaaggtact gttcgagatt actcattctc ttttcaattg
     6781 cttctttgtt atttttccat gaaaaatatt ctttcgcaaa atcctctgta aaactttcaa
     6841 cgccagaagt attcactacc ttctctttta taaccacttt ttcatgagtt gtatgttgat
     6901 caatggcggt gaaattttta taaacgccaa aagcaagact acaagacaat aaaagccaaa
     6961 gggcaagcac catttttcga tgcttaccca cggatacaac tcgttctttt ttcttttttg
     7021 ggctcttttc cttttgattc cgttcaattt ttatcttcat tctttttcct cttttctctt
     7081 atttgtatcg gccagctccg ataagatgct gccgccaata gttttccgtt agattcgtcc
     7141 accccactgg atctcctgca tgaaacatgc gattctcccc tgcgtaaatg cccacatggg
     7201 tcacataagt accggcatca taggttgaat gaaagaaaac taaatcacct ggtttggcat
     7261 cttttagatc aatatgagtc atggcatcat attgatcctg agccacccga ggaagtttca
     7321 ggtcaacttt ggcataacac cattgggtca agccactaca atcaaacccc gtttctggac
     7381 ttgagccacc aaacacatag gccgttcctt catacttaaa tgcctcatca aaaatttctt
     7441 ggactatttt gtcatcaaac tgagttgtag ttaggtattg cttcactaat aaacagtaaa
     7501 acatattgcc ataattatac cgccacccac cattttcctt aatcgcgatt gggttggggt
     7561 aatcgacttt ttgaccacct gatttttcct ttgaaaaaga ttcagctaaa gcaaaactat
     7621 gcttcttccc gtttttcccg acatgatcta gatacccgcc accatagtta taggcctgaa
     7681 taatactatc ttgatcacac cctagttctt tggaacgctt aaccaaagac gcaaaataga
     7741 gacagccttg tttgatggat gcctcggtac ttaatgaatt aggtggtaat ccggctgact
     7801 cagaactttg cataacgtct tccattgtgc caccagattc cacttgaata atcgccaaaa
     7861 gaattggaat atactctgtt atatccgatt ctttggcata cttttccacc attgcacggt
     7921 gtttcaatac ttcttctgag acactagttc cgccaggaga agtgccagaa ctaccatttc
     7981 cctcactgtc ttcaccaaac aacaaaccca cacacatcaa aatacccata aaagagatgg
     8041 tcagaaaagt taatgaaata acacccactt tcttcaagtt cattttttct cacctgacct
     8101 tgattttgga cgatggttca ttcttttttg tactttttga ttggtctgct ttggcagagt
     8161 ggacctctgt ttcacttgtt ggaaagaatt tttcttactt gctgattggc ttttcccttt
     8221 tgaaatgatt tcttgactag ttagttttcg tttgatttcg ggattcagat ttgctctcac
     8281 aatccgcttt ggtgcttctt ttttcagcat catatctttc tgaggttgag ctgctttatc
     8341 tattggaata ttcgcccttc tttttccctg acgatttgtt gcttcactca gtatcttgcg
     8401 tttttcgtcc attttcgcat ttcttcgttg tcgttgtttt tctcgcaaat ccactcgatc
     8461 cgctttttct tggaacagtc cgcgcttgaa ttcttctggt gcttttttcg acttttcgat
     8521 tcctttatgg agattgtatt tcaaattcgt tggcgtattc cttacctgat cctttacttg
     8581 tttagtctta tctttcacca aagcttgggt atccaaaact ttgccgattt ttctcccagc
     8641 caaattcatt cgggattgtt tcttattatt tgaaactgga ttctccttag ttaagtcaga
     8701 tagtatttcg tgatcgttcg gtaatcgtga attctttcgt agagaagaac cggatggctt
     8761 aaatttggat tttgattgtt cctttgccaa cttatttccg actgctgcac caccaccaaa
     8821 agccaaagct cgtcccacat ttcgattgag ttgccgcaat tttcgattca ttaacatttg
     8881 cggttttcgc atgacgcgtc ttcccattga ctggctatca ttactctgga ggttgaacat
     8941 actcataatg tcgcccaatt tgaaatagat accggcaaag gtcacaattt gcagaaaagc
     9001 aaccatgaaa aatggatagg ttgccgaaat attgaaaaac atcgtggaga tgctaaatgc
     9061 agtcgtaatc accaaagtaa cacccgctcg catcataatc gtattgaata aacgaataat
     9121 cgccttcttt cctaaacttt cataagtcgg caacatggac aataagaaac taattggcag
     9181 aaacatcgca aagataatga agagaatttg cgagaacaac ataatccctg ttagaagaaa
     9241 aacaaagaag gaaatgccaa tattgaacaa taccaaaaag accacggtcc ctaaacggtt
     9301 catggtcttg gtaatactga gattcgcatt gtcattgtct tcaatctctg ccttgaccgc
     9361 ttcttcccga tctttgcctt tgttttcatc tggactcaca gatagaatct tgttgacccg
     9421 gtcttcacca atttcttcta tgttggaatc atcaaattgc agtagtaacc aaggttgttg
     9481 gacttgaatc gaaaacaaac tatcccgaat caaatccaca ctatcttttc cttggctttc
     9541 tgaattgggt acaacgatct cggttccaag ggtaagggcc gcttcactaa catctgagct
     9601 aaagtcattg atttttgtga tataggtagg cgcataagca atgaacgaac ccgatagtag
     9661 aaaaatcatc acaaaattaa tcacggctcg aactgctttc gtggtttctc gttttaataa
     9721 tccggtatac gccacataca cccccagagc taaaatcatc aataatagaa acccaaaata
     9781 aaatccggag gtctggagtc cattttcagt aataccagcc aatgtttgga tattctttcc
     9841 aatactttca gccgtatccg aaatgaaatc taatttgtaa gcctcttgaa ccacataacc
     9901 cgtggcgttc gacaaataca aactcaccgt ccaaatgaaa ttagtaatgg cataaagacc
     9961 atacatcacg cttttcccta agccatcacc ccaattccaa ggcaaccaat cccaagagct
    10021 atccacaaag aaatctagtt gatagttgtt cagtgaatat ttggaatata gatttcctgc
    10081 ttcaactgta tcatcaacta gccctgtcgc ctcggctacc gtaccaacta aactcaatag
    10141 aactatcaga gtgagtatgc taataaaaac gattcccaca atccgaaaaa gtttcttttt
    10201 catcccctca ctccattctc tcttgaacag gtggacgggt atcaaaggca tggaagagtt
    10261 cttcaaagac tggatgtacc tgaaccacgc ctacccgacc atacaaatcc tgcatcaagc
    10321 attggccatt ttccaaatct cgtaggcgtt tttgattgct ttcatcttct cgatccaagc
    10381 caaagaattc caaggttttc ttgatttcat gaatatcggt actgcgaaac gcaaacttca
    10441 acccgatatt attcttcaag cgttcatcca gtaaatcatc actattttgc gtgacaaagt
    10501 aaaccccggc attcatagcc cgcccagcac gaacgagctt attcgataat gttttccctt
    10561 gcgctacttg aagaaaactc cacgcctcat ctaagtctac aattttgaag atttctcgat
    10621 tactatggat aaagtccaag gcaaaagtgg aaatgacgat tagcatggaa acactaagca
    10681 attccatggt ggtatattct tcaaaagtgg tttctgcatc tggtaaaacc aaatccgcta
    10741 cttgaatgat atttaactgc ctatctaaac taatcgactg ggtaatcgaa ccatctgaga
    10801 aaagcaaatg ggcaaaatca taatcggtaa aggactcgat gtgatccgcg atattttccg
    10861 caactggtga gccttctttt cgaagctcat taattacttg taataatccc cgttgattgc
    10921 tttgggtaac cgtccgaatg gccttacgca acactggaaa cttctcccca tctcgactag
    10981 aaattcctgt taaaaaggtc agaatatcaa ttgctagact ttcagaatcc ttctttttct
    11041 ccataatcac ataaggatca agtaatcctt gattttttgg cttactagtt aagttgagaa
    11101 tattgatttc atccgcaatt tctggcaagg tttctttcca gccaccccgt tccccttttg
    11161 gatcgacaat cacagcttgt ccaccaaaaa ggacggcgta ataaactaag agattgttac
    11221 taaaggactt tccgccaccg agtgaaccga ggaatgccgc tgctagcgca ttggtaactg
    11281 aatccttgac cccttgtgag gccaatgccg gtttcaggta aacattgcgc cccgtatcta
    11341 cgttataacc aaagtaaatg ccttccaact cccctaacat ctgagtggcg ccaaaaccaa
    11401 gtccggcgag aaaatcagac gtaacatact ggatataatc attcatgtat cgcttactag
    11461 aaggtaagaa ttcttcatgt agccccatca tatcgccaaa aggccggact agttttacat
    11521 tggtatcatc ataaaaatct agcacttcat cacaccggcg ctttagttca tctactgatt
    11581 cggcacttac tcgaaccaca tagctcagtt tgtacatgga ttctttggac tgatccaagg
    11641 tagtttccaa ttcatctacg gaatccaatg catctaagac attggaattt gtttcattgt
    11701 cagattgata ggcatggttg tccaagtctt ttaattcctt tttcttattg cgaacggtag
    11761 caagtgcctt tttgttcgtc acaatctcca cattcatact agtgtcaatt gggaatgtaa
    11821 attgttgttg ctggtaatag aaaagttctg aggagggaaa ctccatttcc cccacaattg
    11881 tattaattgt taagtacgct acatatgatt catgattctc atgttccatg cataagtacc
    11941 ggggcttttc ttcaatcaaa caacgactgg gacgtaataa gtcgtaacgt ttcaccaaag
    12001 tttccgactt cagctttttc tttggtagct gaaattcata ttcttcaaat ggtgtaccct
    12061 tttcgccata gatatgttca atcaaataca tcagatcact cggtgtgact cgtctgactt
    12121 taaatcttcg agccaattta ctttccatta gtttttcaag cttggtatat cggcgaattt
    12181 cttcattgga taaggaaaca aaatcgccca tcaaatgatg attcactcca tagacaaatt
    12241 cttgaaagcc ggagaagaaa gatttcttga gattctttag attgacctct tcatctgtcg
    12301 caatcagttt gaagccaata aaaaagcgat aatcaatctg actatcgcca atcatggata
    12361 ctaatgcctc tgtctgtaaa tcaattcgtt gtttcgctac ttctgttaat cgcccggtaa
    12421 tttccttttt tgatcgttct tgcgtggcac gaatactact ttctgtggca atttgtaagg
    12481 catgaatctt gccttcacga ttttgcgcaa tcaattgacg aaagttatcg tggacttgat
    12541 atttctgttc aggtgacaaa aaggagtaat tgtaaggtat taactcatag taggcaaaac
    12601 attctccctc ttgattaaag acaagattat tttcaatata cttaatcggg tacatagttc
    12661 acactcctta ccagagtaat gggttcgtcc catcgtttct tctgttcttt gactttcttt
    12721 cctgcgaaag tgaccttaaa tcgtaagtga tagctaataa aagatcgtaa aaatcccaat
    12781 ggtttcttac cgtcaaacgt tttttgcgac atgaaccaag taactgccac cggtatacct
    12841 aagtatttta aaaaggcccc ctctatcata gatagtggag gcacatgggc aaatacgatg
    12901 acaaacagca aggataaaac aaaccaagcc atctggttaa aagtcacagg aaaaggcagt
    12961 tgaaagtcat taattgcata aatgaccttt tcaactgccc aaatactggt gtagcttttt
    13021 attttcttca tcaaatcatt cctttaaaaa gaaggatagt ttagaaatca actacccttc
    13081 aataattttg ttaatatgga tactcaaaaa cacctcgact cgttaccaaa taattgcctc
    13141 caatctccaa gtctctacca cacgcatcaa aatcaatata cgtcttcaag tgttctggta
    13201 cttctccaaa atcaccagct tctacaatca agaaatatgc tacatcactc atatcatcgc
    13261 aatctggata atagataata tcatccacat gctctaccat ctcctcaaaa gaattgaaga
    13321 aagcatgttg aatctcagaa gcaacttcac caactggcgt tccttcaagt tcctccgcta
    13381 agccacacaa ccgattaatt tcctcaattg gcgtatactc atcaatctca aagggaagtt
    13441 cataatcatg aattgcatat tcctcgtatt cgtcatttaa cccaattcgt tctttcactt
    13501 catcaaaatc gacaggtggg gtaaaccacg cccccaccaa ttcaccttcg ttatatttcc
    13561 cgaggttcgc aatataaaca cgcatctgtt ccatcgtgct tcccccttac gcaccgacaa
    13621 ttcggttaaa caaactgagt aagacatcct tcaccccaga agcattaaag accaatccga
    13681 ccgcaatcaa agcaatcacc aaaaagccaa tcagtttgga aaactcccgc ttaaatccta
    13741 aaaacaaacc aatgaccaca attgccagta agactaagga ttgggcattg gttaaaaacc
    13801 attgatatat ggataggtaa gttcctgata ttataaatat ctcaaacttt tccccccatt
    13861 cacacggagc gtgcgacttt caccgcactc cgcgttccat cgtgtttata ttgaaaagta
    13921 tttatgtagt agacctatat caagtttaat tcttgattgc cagctaatac tctatattta
    13981 tttagtctta tgagctggac tttatttagc ttcaatagag ctatatattt ttgtattgtt
    14041 tcttctgttt tagcgtgtat tagacggtgt accgcctctt ttaccaaaat aagattttta
    14101 taatcgtcat ttccaccagc tccagttggt acaatatgat gacaatgtat ttcattagct
    14161 tgttgaaatt cttccagagt tatagcacat tttccatgtt gagcagaaaa tagcgaaatt
    14221 cgattgtctg cgtattctat tgtgcgattt tccaaagata atttcattaa ctggtacatc
    14281 agtaatttat caacacttag attggtgtgg ataaattttc ttccctctag agtgtagcaa
    14341 ttcgctaaat acacctttgc cattggtttt ctgtgttgga tatatccaac agggaaaata
    14401 ggttcgccct cgccagcaac ataccgaagc attttagatt taccatatct tgttgcctcc
    14461 tttgtcgtaa gttgtctgcc tgtttttctc aatctgcccc ttttgtttgt acctaatctg
    14521 ttagtgaaga tagccattac agctctattc agtttcatac aatccagatt tacattcgtt
    14581 gcaatctgat aataattttg catacctaat accattgaat tataaagtct tgtttcttct
    14641 aattccgttt tcccttttga gggagaagca attttctttg cctgtgatac cagcttgtct
    14701 ttttggtttt tgaattgttt atcacttata tgtgatttaa caactaactt ctttcttctc
    14761 ggtatcattt taatcttgaa accaagaaag tcggagtatc tttttctaac atttacaatt
    14821 cttgtttttt cttgtgatac ttccaatttt aatcgttgtt caatccattg agtgattgct
    14881 attttggctt tttcagcact ttccttgtag cggcagaaaa ctctaaaatc atctgcataa
    14941 cgaaccatat acatttcctt taactttgtt ttcttcattt ctttgtaagc gttgctttta
    15001 catgggctac cgcttttatt gatatgaacc acatagtttt ttgtaacagg attttcctgc
    15061 cattggcttt ctacccaatg gtctaactca ttaagaacaa tgtttgctag tagtggggat
    15121 attattcccc cttgcggagt tcctttctct ggatatgtta ttgtaccgtt agtcattttg
    15181 attggagctt ttaatatcct ttttaaaatg aatatcaagt gcttgtcccg tattcccata
    15241 gcccatatct gttttatcag cttggaatgg ttcacgttgt caaagaaacc cttaatatca
    15301 aattcaatca cataatgtag ctggctgatt tgtaggcggt tataggttgc ttttattgca
    15361 ttttcaactg acctattagg tctaaaacca tagctgtttt cactaaattt agcttcgcag
    15421 attggttcta atacttgttt tatacattgt tgtataagcc tgtcccacat acaaggaata
    15481 cctagaggtc tggttttacc atttggtttt ggtatttctt ttcgcctcac tggcttaggt
    15541 cgatacccat gtggactgcc tgcaacaatg tatcttactt ttttgacaac tgtttctgct
    15601 ggcaatttgc caatatcttt aattattgta ccgtctgttc ctgctgtttt actgcctgca
    15661 tttgctttga tatttcgata tgccagtaat atattttctc tcgataggat aatatccata
    15721 agatgagtga aaatatctcc attcttactt ttggcatata aatcatcaaa ggtgttttgc
    15781 atttcgtaat attcagcgtg tcttaaatct tctacacaca atgtttcatt tttattttta
    15841 ggcataaggc ttcactcctt tacttgaaat tgagtgagct tttttatctt actcgtaatc
    15901 cttattgaaa caacataata cttttctaat atatacacga cttgagggca ttcctccatt
    15961 ctcattacaa gaatttctta ggttcgccct ctgcttttca gcattagttc aactgcttat
    16021 tgttcttcgt cagtttattc tcttgatgag aacccaatac ctttcctcgt tccgataatc
    16081 ttatctatac atatatatcc ttaggtcact agctataagc ctgctggctt gataccgcct
    16141 gtaacgttat atggcatttc ataacctata ttcttacttt gccacaccag ctacgatttc
    16201 tcgtaccttt gcatttctac aaagttcaaa tttagacccg tacattcgct gtttcgtcag
    16261 ttccttatag aacattctaa ccatagatat tttatagacc cccgacctgt tggatacgct
    16321 acccacctct ggatagtttt cgatagaaga tatatgttca tcaactatta cggtatcttt
    16381 cagccgtctt taccgagctt caaacattct gagtttcctc atacaatgct tgtcggaggc
    16441 tcagggcagg cgtttcagag cgttacttca tcattccacc tgtcagatta tcagttctcc
    16501 taataaccaa actttttcgt tcttttatta gtgccttagc ttttcactaa ggaacgagtc
    16561 gcacataaat tttgtccgaa attcatcttg tcactttcct ttcttttcca ttgctcgatc
    16621 cgctgctgca cttgcattca tcaagcacat cacagtaaca ccaagcatac tgccaccaaa
    16681 taccaaaaaa atatctttta caattcccat gacgattcct cttttcatat aatcaaattt
    16741 tcaattgcca tactttgttg ctccaaaatc tttttatgtc ggtctgataa gcgtgcattc
    16801 tgtaccatat cctggatata ggttgtccga ttcaatcgat ccaactcctt agccattttc
    16861 cacgtagggg cgacttgtcg gcctagccaa ttcaaggttc gttcaatggt atacggtttt
    16921 ggctcggaag ttaattgaat ctctttccga ttccgtccaa taaaacgccc ccattgttca
    16981 ttcaagggcc agttggttcg ccttttacct tctactttat ccgcaaatcg caagtaacga
    17041 ttgataatgg aaaacgtggt gctctcaatg ctacgacctt ttaacaaatc ttgaatcgca
    17101 tgaaaagcac ggtcattttt cagtcgaatc tcaaaacgat ttttcgtctc agtttggtct
    17161 aatggaatcc ctaacttgat atattgttca tagtcttttt catacaaaca gaaataaacc
    17221 tcactcttga gactgccgat atataaggta ttgcccatgc cgtccttttc atcagccttt
    17281 aaaagttccc cggaacgata acttttaaag gtacgaaaca aggagataca ttcttccttc
    17341 tgacattttt tcgttagctc aggtatatcc aatagcccca ctcgatcatt gattgccaag
    17401 tccaagcgtt tcatcacgcc accggccttt agacaatcct cgaaaaagtc gtaccagcta
    17461 cgcttttgag cgagtaaaaa agtttcaaat tgtcggcaac ctcgcccctt taattctaaa
    17521 agcacacctt tatcttcttc atgagacaac atcaccacaa tatcacccat gacatactgt
    17581 tcttgatagg agtaaaaggc ataatcttca tggagcatat actcaaattt caaatggaga
    17641 atttcttgaa taatttttcg tgcatccgtt gtgggaaagc gaatccttac atagtcaaac
    17701 agtaacgtca aaggcaaatc gggattaaag ctttccagtt ggtcaaaaat tttcctcatg
    17761 gtttttatag atgcggttac gccaccattt tctatctgat ttaagtacgg ccgactaagt
    17821 ccacaagccg tagccaattt attttgtgaa acgccgtatt cgttgcgtcg gtcttttaat
    17881 agccgataat ctaaattccg ttgtgccaag ttctcacctc caaattcaag gtgttaactt
    17941 tttcaaatga gttaacacct atacaaatta cttactatct ttgatacaac aagcattctt
    18001 aatggttgct tacttatttt ttgtgttttt gtacccccct gttagatacg ggggtgagac
    18061 atcatggtcg gctaacgccg tccatgaaca ggcacagcac cacggaggat ttctgcttcg
    18121 cgaatcctcc gcggtcagcg cctaacagtc agttgtgttg gtttaggtta ctcatctgta
    18181 ataccattta cttccaactc tgctttcatt tctaacaagt attgacgaat atctggttct
    18241 tttgcttggt ccgcaatccg ctcaaattct gcaattactt gatttatttc aattcgagca
    18301 tccgtctcct cattcattaa atatggatcg ccagcgactt tgacaccatt cttgcgaata
    18361 tcaaatttta accgtcgcaa ttgttcttct aattctgggt ctgactgaat ctttccaatc
    18421 acccaataaa tttctccaat caactcatca taaaccatgt acatccaact cttttaatct
    18481 tctggcaata tcaaaaatga ccggaactgt tacagagttt ccggcttgct tatacaactg
    18541 actatctgaa ttgacttgag aatctcgatc aaaagcccaa tcgggaaagc cctgaagtcg
    18601 ccaacactct ctaggtgtta gcttccgaat ttgatactga tccgtgacga tcgcttgttg
    18661 gtctcctgtt agtaaggtat gaacactctg ttttcccaga cgacctcggc gacttgtgga
    18721 attaggaaaa gccagattca cactgtcttc tggcttcacc tcttgatatt ctgtttcatt
    18781 ttttcgaatc atgatttttg taccaccttg tttcgttgac aaggtggggg cggttccagt
    18841 aacttcataa actcgattac cttgttcaaa ctttcccggt aatagtcctg ctacagagat
    18901 tccatccctt gctggcagtg cgattttgat tccttcatca cctgttaagg ttggcgctac
    18961 gccatctgaa ccatagactt gtccacccat tcctcgattg gaaggattga tatttaccaa
    19021 atttctgatg ttttgatcag atgttgaaga cggtccaatg ataggaaata cttgttcggt
    19081 acatgcgtct cgagaatgtc cgataaggaa tatccgctcc cggctttggg gaatgtaggc
    19141 ctttgagtta agcacctacc actccacgtc ataccccaat ccatccagcg ttcggaggat
    19201 cgtctcgaac gtagcccctc cttcgtgatt gagcaatcct cggacgttct caaggaataa
    19261 aagcttaggt cggagaagag tggtgaaccg agcgatttca aagaataaag tacctcgagt
    19321 atcggaaaat ctttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccgccgca
    19381 aaggatgtcc actggtccga gggattgaat aaactcatct gatacacttg tgatgtcatg
    19441 catttccacc tccttacttg tatcatgaat tgctttataa ctacgtctag caaatttatc
    19501 aatttcacaa aaaccgatac aatgatggct cgcctgttcc atacctaaac gaaagccacc
    19561 aataccggcg aataaatcta aaaatctcat acattacctc attcattgca aagaaaaaag
    19621 ccaagaacat tttgctcttg gctcatacat tattaatt
//
LOCUS       DACEDO010000054        19450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEDO010000054 DACEDO010000000
VERSION     DACEDO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.054"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            126..3653
                     /locus_tag="IWO52_002002"
     CDS             126..3653
                     /locus_tag="IWO52_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0356261.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="IWO52_002003"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="IWO52_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0356262.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="IWO52_002004"
     CDS             7441..7782
                     /locus_tag="IWO52_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0356263.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="IWO52_002005"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="IWO52_002005"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0356264.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="IWO52_002006"
     CDS             9139..11559
                     /locus_tag="IWO52_002006"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0356265.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IWO52_002007"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IWO52_002007"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0356266.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="IWO52_002008"
     CDS             12993..13730
                     /locus_tag="IWO52_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356267.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="IWO52_002009"
     CDS             13744..14559
                     /locus_tag="IWO52_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0356268.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="IWO52_002010"
     CDS             14572..15219
                     /locus_tag="IWO52_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0356269.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="IWO52_002011"
     CDS             15235..15891
                     /locus_tag="IWO52_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0356270.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="IWO52_002012"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="IWO52_002012"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0356271.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="IWO52_002013"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="IWO52_002013"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0356272.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="IWO52_002014"
     CDS             18233..18751
                     /locus_tag="IWO52_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0356273.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="IWO52_002015"
     CDS             18858..19358
                     /locus_tag="IWO52_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0356274.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6540 a         3340 c         4177 g         5393 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgtgcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc
//
LOCUS       DACEDO010000055        19433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEDO010000055 DACEDO010000000
VERSION     DACEDO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.055"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            170..364
                     /locus_tag="IWO52_002016"
     CDS             170..364
                     /locus_tag="IWO52_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356275.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            633..932
                     /locus_tag="IWO52_002017"
     CDS             633..932
                     /locus_tag="IWO52_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356276.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1029..1463)
                     /locus_tag="IWO52_002018"
     CDS             complement(1029..1463)
                     /locus_tag="IWO52_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0356277.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1695..2405)
                     /locus_tag="IWO52_002019"
     CDS             complement(1695..2405)
                     /locus_tag="IWO52_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0356278.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2395..3255)
                     /locus_tag="IWO52_002020"
     CDS             complement(2395..3255)
                     /locus_tag="IWO52_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0356279.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3260..3898)
                     /locus_tag="IWO52_002021"
     CDS             complement(3260..3898)
                     /locus_tag="IWO52_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0356280.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3913..4212)
                     /locus_tag="IWO52_002022"
     CDS             complement(3913..4212)
                     /locus_tag="IWO52_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0356281.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4255..5739)
                     /locus_tag="IWO52_002023"
     CDS             complement(4255..5739)
                     /locus_tag="IWO52_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0356282.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5759..6220)
                     /locus_tag="IWO52_002024"
     CDS             complement(5759..6220)
                     /locus_tag="IWO52_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0356283.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="IWO52_002025"
     CDS             complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="IWO52_002025"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0356284.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7523..8293
                     /locus_tag="IWO52_002026"
     CDS             7523..8293
                     /locus_tag="IWO52_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0356285.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8566..9393)
                     /locus_tag="IWO52_002027"
     CDS             complement(8566..9393)
                     /locus_tag="IWO52_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0356286.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9961..11196
                     /locus_tag="IWO52_002028"
     CDS             9961..11196
                     /locus_tag="IWO52_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0356287.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12195..12455)
                     /locus_tag="IWO52_002029"
     CDS             complement(12195..12455)
                     /locus_tag="IWO52_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0356288.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12482..13783)
                     /locus_tag="IWO52_002030"
     CDS             complement(12482..13783)
                     /locus_tag="IWO52_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0356289.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14075..14533)
                     /locus_tag="IWO52_002031"
     CDS             complement(14075..14533)
                     /locus_tag="IWO52_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0356290.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14546..15871)
                     /locus_tag="IWO52_002032"
     CDS             complement(14546..15871)
                     /locus_tag="IWO52_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0356291.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15888..17300)
                     /locus_tag="IWO52_002033"
     CDS             complement(15888..17300)
                     /locus_tag="IWO52_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0356292.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17317..18081)
                     /locus_tag="IWO52_002034"
     CDS             complement(17317..18081)
                     /locus_tag="IWO52_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0356293.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18185..19060)
                     /locus_tag="IWO52_002035"
     CDS             complement(18185..19060)
                     /locus_tag="IWO52_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0356294.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19315..19399)
                     /locus_tag="IWO52_002036"
     tRNA            complement(19315..19399)
                     /locus_tag="IWO52_002036"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19363..19365),aa:Leu,seq:caa)
BASE COUNT         6182 a         3993 c         2993 g         6265 t
ORIGIN      
        1 tttaagaaca atagcctttt attatatata ttattcaaat tagttatatc gagtgaaaaa
       61 aactttcaaa ttgattcttt ttatttatca taaagatata gatacataat cattttcact
      121 tacatatatt tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt
      181 agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata
      241 tcctttactt ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat
      301 aaagaacgag ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa
      361 gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga
      421 ttatagaatt agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat
      481 accatatgat tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga
      541 tgaatcaaat atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt
      601 tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg
      661 aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca
      721 aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg
      781 caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg
      841 ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat
      901 ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat
      961 actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt
     1021 attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa
     1081 gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt
     1141 ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc
     1201 aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg
     1261 atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc
     1321 ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt
     1381 gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg
     1441 atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata
     1501 atacagttct ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat
     1561 tccttaaaat tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga
     1621 taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc
     1681 ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt
     1741 ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg
     1801 gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc
     1861 ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat
     1921 tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt
     1981 acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga
     2041 gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa
     2101 tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg
     2161 agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc
     2221 accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact
     2281 cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc
     2341 ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa
     2401 gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc
     2461 tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc
     2521 tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac
     2581 atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc
     2641 agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga
     2701 caaattgcct aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc
     2761 taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat
     2821 gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga
     2881 attcattgat ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct
     2941 tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc
     3001 tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg
     3061 tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa
     3121 tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc
     3181 ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat
     3241 cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg
     3301 cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat
     3361 cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa
     3421 agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag
     3481 ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat
     3541 ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt
     3601 tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc
     3661 ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga
     3721 ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta
     3781 agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc
     3841 ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct
     3901 tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat
     3961 ttctttgact gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg
     4021 agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact
     4081 ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat
     4141 tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac
     4201 taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac
     4261 tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt
     4321 tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc
     4381 ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat
     4441 cccgatataa ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc
     4501 tacttgtatg attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt
     4561 attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac
     4621 gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc
     4681 aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc
     4741 aattcctgga acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag
     4801 ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa
     4861 agaagtcgtc ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat
     4921 caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga
     4981 agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat
     5041 ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc
     5101 taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc
     5161 ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc
     5221 taaaatcagt cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag
     5281 taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga
     5341 acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag
     5401 taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac
     5461 agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag
     5521 tccaaccaag attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa
     5581 gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa
     5641 aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt
     5701 tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt
     5761 aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct
     5821 caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt
     5881 ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt
     5941 cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac
     6001 catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac
     6061 cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat
     6121 cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg
     6181 ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta
     6241 taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata
     6301 aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca
     6361 gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat
     6421 ctcttttgga tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt
     6481 tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc
     6541 tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt
     6601 ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc
     6661 gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc
     6721 ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt
     6781 gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat
     6841 tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca
     6901 attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac
     6961 gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa
     7021 attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat
     7081 gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc
     7141 ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg
     7201 atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc
     7261 tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc
     7321 atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt
     7381 ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat
     7441 aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga
     7501 tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa
     7561 agaaattttg gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc
     7621 ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga
     7681 gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg attttgatca
     7741 atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga
     7801 ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca
     7861 atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga
     7921 taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg
     7981 cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag
     8041 cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga
     8101 aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg
     8161 actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa
     8221 attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt
     8281 agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa
     8341 caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg
     8401 tatccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc
     8461 taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt
     8521 tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt
     8581 ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat
     8641 atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag
     8701 caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca
     8761 taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata
     8821 tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc
     8881 cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc
     8941 agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata
     9001 aaataatgct gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa
     9061 aatttgattt aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt
     9121 ataagattct tggtattttg atacagaatt caatgattga atctcattta tttttttcct
     9181 gattttgtct tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca
     9241 aagcgcactt aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt
     9301 tgcttgttca caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta
     9361 ttcttttagt aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta
     9421 tttctccttt atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga
     9481 ataaaaattt taattttaaa ataatattca aattaaacaa ctggcgtatc tgctccttga
     9541 tttctttttg tgttatctga ataattatcg tttacagtta taacacatag tgaaaattgt
     9601 catggcgaga aagtctaaga atatttttaa acataactat ttctttctta cgtgtgtata
     9661 atgacgacag cacacctatc aattaatata attattttat ttttttatgt tctcatgttt
     9721 atttttaatg acacaacttc tcgtatttat ctggcacaac atataaatcg atcaaaaaat
     9781 tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta aatttagaag
     9841 ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac tcgaacgctg
     9901 tgttttttag tatactagta agaaacgaag ggatagactc attccaataa ggaggatttc
     9961 atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac acgaatgatt
    10021 gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt ttctaaaact
    10081 tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc gatccttacg
    10141 aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa aaaccctttt
    10201 accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc taagtttgag
    10261 cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg caataaaaag
    10321 attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca atcagagcat
    10381 gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct ttggcactgg
    10441 acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca cgctgcacaa
    10501 aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga tctaaatctc
    10561 atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt
    10621 ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac ttttatacag
    10681 cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc aggcaatccg
    10741 cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc ggttattgca
    10801 cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt aataacagaa
    10861 tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca cggcggggaa
    10921 gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg gatgcaattt
    10981 gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa
    11041 ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc caagattcgt
    11101 cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca agcaattcaa
    11161 gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa agctttttta
    11221 atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata caaaaaaagt
    11281 aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa cacaaaatca
    11341 tccttaaaat aaaagatctg acgctatcca aatgagcata ggtagcgtct gccatttatt
    11401 cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt cttcaaaaga
    11461 aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga tctagaagca
    11521 tcgctttgta gagatttgtg ccgctagtcc cctcaacaat tactaaatca aatgtaggat
    11581 ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga tctcgcaatc
    11641 cgatgcatac tcctatataa actgccagca atccccacat attgagtaga tcatgtcaga
    11701 atgttctatg tcgtcagcaa cgatacacta aaagtcacgt tcaccatgac tgattgctat
    11761 tcccagcaca attatttcta tacacgtttc atgatttgaa taaacactca catcatacat
    11821 attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc ccaaatctat
    11881 atttgacttg atcactttgg tagtagatct actaattcta aatccattgc tgataaaatt
    11941 tccatttttt cctccttctt tctgccaaaa cagttaaaac aataataatc tttatgtaaa
    12001 aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg tggtcaaaag
    12061 cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt
    12121 taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt ctgttacaac
    12181 cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc gatcataaaa
    12241 ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac attcatatgc
    12301 ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca aatccatcga
    12361 ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc cgttaataaa
    12421 aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct aaaaaccaaa
    12481 cttagacagt ctggacttcg ttttctccac gactttgttc atctgacagc atctttttat
    12541 cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg atcatgatca
    12601 agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga gctggtaatg
    12661 tccatgttgg caaaacagta aatctattga ccatttctaa tttgactgca aaataactga
    12721 taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga ttcaacatca
    12781 taggagctcc aaaaattatg ggctcattga tattgaagat accaggaata atagaaaaac
    12841 gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca gataccacga
    12901 tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca ggagcgatat
    12961 agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca tccaacatga
    13021 tcagccacat tggacgaaca acagccccta caatactcat gccatggata ccaaatgacc
    13081 acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc cctaaaatat
    13141 tggttaaagg ctcgaataac gataataagg cagcgtttac atcaaagccc gcgacgcttc
    13201 gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca aaactacggg
    13261 caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt ttaaagaaac
    13321 gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca gatgctccca
    13381 aagaactcat aggtaagacc cagccaagag gtgcctcttc aggagttaaa acgtttacag
    13441 gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca ctatctaatc
    13501 catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc atcaatccca
    13561 tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg ggcaataaat tcattccatc
    13621 caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc aatgacaatg
    13681 ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt tttgccattg
    13741 gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc actccttttc
    13801 aataaaatat gaaccattca ttgcatgaca acgcaaagaa tgttcataac taattatata
    13861 attaagaaat tttatttcaa tatattctct taaataatca aatttatttt taagaatcct
    13921 gaaaataagt tttctaaatc ataggagcaa caaaaaaatt cgataattaa caaaaatcaa
    13981 aatgattaaa tctagtgatt gaaacatatc atttcaaaaa aattttgtaa attcacccat
    14041 aaatatcacc ctatttaact tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa
    14101 tctttacttc ttggttttgt tttaataaat ttccttcaaa aatgcctgga gaaactacaa
    14161 tgatcaccgt atctttttgt gcttgctcca acttcttcaa atctataata gctaacagtg
    14221 tcccttcttt tacctcctgg ttactttcaa cccgtatctg aaacggactt ccttcgagtt
    14281 caactgtatc taaacccata tggatcagta tttttctgcc agattcactt tcgatcgtta
    14341 tggcatgtcg ggtaggaaat atattatcga ttattccttt aactggcgca tggatatatc
    14401 catcatgatt cgttatagcg tatccaactc ctatcatttt tgaagaaaag acctcgtctt
    14461 ttactgctgt taatggaata atttgaccac tagctacagc ttttagaatg tttcttgttt
    14521 taaagaaatg cactttttat cacctctact ttaatgctgg ccaatatttt ttgtttactt
    14581 tgatcatttc atctagtatt ttttttgcga ctctggcatt tggtacggtt tgattcaatg
    14641 taaatgcttg taaagctttt tgataagaac cttcaaagta agcatctact agtaacttct
    14701 ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc actgattggt tttcttagtg
    14761 taattggctc cacaccagtt gcaccaacat atgccggtat ttcaacaact gcatctgacc
    14821 gtagattcgg aatcgcccct tgattaggaa cgattagcat aaagcgacga cgatcatcat
    14881 tcaacaagga aatagctata tcgacgatat attgtccatg ctccccaaaa ttaaagttca
    14941 atacttcttc cacaataccg cggcgtattt tatcagccat ttctttggta tttttctctc
    15001 gtccatccat gaccatatta gcacgtgtat actctttgtc agtatgctca acgaccatat
    15061 caggatataa gtaatactct aagtaattat tcgggatatt acttggaaaa tttttcgtga
    15121 tgactgacat catttgaaag gttttttgcc aagttttatc tcctatatta aaatcggcga
    15181 cttgcatttc ttttgttatt agtttttcaa taatctctgg cataatttct cgttgcaatt
    15241 ctttatcata aatcgatgta taccagccaa agtggtttaa accataatac gtaacgatcc
    15301 aattcttacg atcataaccg taattcactg caatcgtttc ttcaattgaa atagtcatat
    15361 cacaggcatt aatcattttt acattcggat atttacgacg aacagcttca gaaacgatcg
    15421 actctggatt tgtataattc aaaatccatg ctttgggggc atagtcttgt atatgatcga
    15481 caatttccaa taaacctttg attgagcgca tcccataagc aaatcctccg agtccacacg
    15541 tttcttgtcc tactaatcca tgtttaagtg gaatcttttc gtccttttct cgcatctcca
    15601 atcctccaac acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca agctttggat
    15661 catccgttgc acgaatgcga atatttgaaa aaccttttct tttcagcata aatttaacaa
    15721 tcaagtacat atccatattt ctttcttcat caatatcata taaacgtatt tcagataatg
    15781 gcaaccgttt ttcattgttc aaaatggctt gaacgattcc tggtgtatac gtactcccgc
    15841 ctccagctat agtgataatc tgttttttca tcatcatctt ttcctttcta ttcttctaat
    15901 aatttattga attctgccat aacataagtc acttctaagc cgatgataat ttgataattt
    15961 ttttcagttg gtttaatgat cccgcttatc cctaattgct tgattcgctg ttcctcgacc
    16021 atggtgggat ccactacctc tacccgtagg cgtgtattac aactatagac atccaggata
    16081 ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca tatattcata atttttactt
    16141 gctaacccta actctgtttc ttctaaggtt acttcattgc caaactctgc cttagcttca
    16201 cgtcctaatg tctgaatgtt tctcttattg atcaaataat aaaaagtaac gtaatacaag
    16261 gcaaaaaaga aaattccgat tggaagaatc aaaatagcat tggtagcgat tcgaaaattg
    16321 atgagataat caacaataca tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa
    16381 cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat aggagaaaga
    16441 aacatgaaac taaattcaac aggctcagtg atatttgcaa taaatgaagt tcctgcaccg
    16501 cttcccatga ttcctttgac catctcttta ttttctttat aagctgcttt atagatagct
    16561 agacatatag ctggtatacc aaacatcatt actacaaaaa aaactgataa aaacagtcca
    16621 gcctgaggat ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc cttgctagga
    16681 gatgtatagc ttcctaaatc gtaataaata tacgtattca acacatggtg cagtccaaag
    16741 gggatcagca aacgattcaa aaacataaat atccccacac ctaatgctcc catatcaacg
    16801 agcagttttg caaaattatc aatcccaatt tggatgagcg gccagataaa gcccaaaaaa
    16861 acagctgcga ttgtttgtag aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa
    16921 taagaaaaaa ctactggtaa tttttgttct ttaaagcgat tatacgtcca tgccgctact
    16981 aaaccggaaa taattccacc aaaaatcccc atattcactt ctgggtccat aatttctaag
    17041 ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta
    17101 tttttgacaa aaccgattgc aactcctata gcaaataaga tatccaagtt tccaaaaacg
    17161 gcatttccag ctgcaccaat tactgggatg ttcagcatat cgtcagctga taaacgcatg
    17221 atcaatcccg ctaatggcat cgctgcaact ggaagtagca tcgctcttcc aagcttctgc
    17281 acatttgatt taacattcat ttttattcct cctctattaa atatcctcat atgttactaa
    17341 ctcaatcaaa ggatcatttt tcactttttg agcaaatgct gaatgaatta aaaattcgac
    17401 ttggtgggta cgaggataca caaaacttga accatttaac aattcctgat ccaaatacgc
    17461 tgcgtgacac atgacttcga tcgttttatc ttctctcaat atcatgagta aacttgccaa
    17521 atattcttcc acactcgtat tgatttgtcg ttttttttca tcagtttctc ctacgacatc
    17581 gaaacaaggc tcaaaataat cgacatgacg aacttcatct tttcgttcaa agttgctccg
    17641 aaccggcaaa tcatattttt ttgctaatgt aacaactacc tcctgatttt catccaaagt
    17701 atgagtatga tgatgactgt ctaaatgagt tggtatgatc cccgcatgat aaattttttg
    17761 aatctgagca ttccattcct gatacagttg atctttgtca atgttaaatg gatgctgata
    17821 ataagataag gatctgaact ctccgctttc gataagagta tcgacagttt tgaggagtgg
    17881 tttgccacac gtcaatgtta aatggactcc tacacctagc ctaggcattt cctttcttag
    17941 tttcacagca tgctcgaaac ccggcatatt tgccatcaat gtagttgatg tcaaaattcc
    18001 tcgctgataa gcatccataa tcccataatt cacaccatgg ctatagccaa aatcatccga
    18061 attaaaaatc acttttccca tttcattctc cttctttttt ctactaaaat aggcgcaacc
    18121 aaataatcat agtccattta ctatgtgtca tttggttgcg cctatagata gttacgccct
    18181 tattttaata gattcttgct agatgaattg ccaaatagat gatttcattt tcttgaatgg
    18241 caagttgaaa ttgctgttcg atgatatctt taatctttag tgctgtacaa tacgctttgc
    18301 tatgtttaaa tgtttcagta aaaaaaccaa tattttctac tggcgtttct tcctgataaa
    18361 tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg atgataagtc agtgattctt
    18421 tatcataaac gtttgaatag gatttttcta gatgggacaa aatcgtggtc gtcatttcta
    18481 ctatttttaa gctctttaaa ctattaggct ctcctagctc agcattaatg atatgcatag
    18541 tgatgaatcc tacttcattt gaccctaact cggtttgata tcgatgattg attcgctcga
    18601 ccacttctac tgctatatca tattcatttg gataaaggaa tctcatttct ggcaacataa
    18661 ttgctgtgac atctctatct tctctttttc gttgaatagc aaaataaata tgatctgtca
    18721 atgtaatata gatatttgaa tctaatggag aggagatcct agctgttatc agttcaatca
    18781 cttcttccgc aaaatccata aattcgattg gaatctgcga gactatctca ctgaatcgtt
    18841 tgatcttttc tttagaatct aatacaaata atttgtcagc atcctcaaca tcaaatggct
    18901 tactcacttt agcagaaaag ccaatccctt tccccatcgc aattttttct attccatcgt
    18961 catcgatcaa tacagcatta tgattcagct ttttcatcac atacaccgtt aattcacctc
    19021 ctctatatga taaaacaaaa caacttgcag actccttcat acatattttt gtctccaaac
    19081 aatttttaaa gacaaaaaac gacctaagaa agaacccttt cttaggtcgt gcctaattga
    19141 atagtagcac cctacataat ttacaaaaat agtttaacac ataataaaag cgttatcaat
    19201 atctttttta ctcgttttat gataatactt gtatcgaatt gatttggagg taattacata
    19261 aaaacaaaaa aaatatagca actctctata agaaagttgc tatattgata attataccgg
    19321 cggccggggt cgaaccggca cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg
    19381 ccaattccgc cacgccggca aatttaaaaa taataactgg ggtagctgga ttc
//
LOCUS       DACEDO010000056        19200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEDO010000056 DACEDO010000000
VERSION     DACEDO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.056"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(50..1348)
                     /gene="asnS"
                     /locus_tag="IWO52_002037"
     CDS             complement(50..1348)
                     /gene="asnS"
                     /locus_tag="IWO52_002037"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0356295.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(1388..2578)
                     /locus_tag="IWO52_002038"
     CDS             complement(1388..2578)
                     /locus_tag="IWO52_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0356296.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(2599..3126)
                     /locus_tag="IWO52_002039"
     CDS             complement(2599..3126)
                     /locus_tag="IWO52_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0356297.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGYESGDEVKTIKGI"
     gene            complement(3173..5962)
                     /locus_tag="IWO52_002040"
     CDS             complement(3173..5962)
                     /locus_tag="IWO52_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0356298.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKATLKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(6109..6309)
                     /locus_tag="IWO52_002041"
     CDS             complement(6109..6309)
                     /locus_tag="IWO52_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0356299.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(6761..7081)
                     /locus_tag="IWO52_002042"
     CDS             complement(6761..7081)
                     /locus_tag="IWO52_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356300.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHILHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(7584..8702)
                     /locus_tag="IWO52_002043"
     CDS             complement(7584..8702)
                     /locus_tag="IWO52_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356301.1"
                     /translation="MAKKDKELLSQLISEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FSKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            9175..10482
                     /locus_tag="IWO52_002044"
     CDS             9175..10482
                     /locus_tag="IWO52_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0356302.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKVSGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            10600..11799
                     /locus_tag="IWO52_002045"
     CDS             10600..11799
                     /locus_tag="IWO52_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356303.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRVIFSSNNYHIF
                     RAGLYACQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(11826..13094)
                     /locus_tag="IWO52_002046"
     CDS             complement(11826..13094)
                     /locus_tag="IWO52_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0356304.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DTRLSHPWLLYLLPVSGALFAYLYVYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(13257..13904)
                     /locus_tag="IWO52_002047"
     CDS             complement(13257..13904)
                     /locus_tag="IWO52_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ0356305.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELLEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(14312..14815)
                     /locus_tag="IWO52_002048"
     CDS             complement(14312..14815)
                     /locus_tag="IWO52_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0356306.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(14938..15702)
                     /locus_tag="IWO52_002049"
     CDS             complement(14938..15702)
                     /locus_tag="IWO52_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0356307.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            15809..16084
                     /locus_tag="IWO52_002050"
     CDS             15809..16084
                     /locus_tag="IWO52_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0356308.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            16680..17630
                     /gene="yidC"
                     /locus_tag="IWO52_002051"
     CDS             16680..17630
                     /gene="yidC"
                     /locus_tag="IWO52_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0356309.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(17809..18783)
                     /gene="lacD"
                     /locus_tag="IWO52_002052"
     CDS             complement(17809..18783)
                     /gene="lacD"
                     /locus_tag="IWO52_002052"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0356310.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDTTIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGILCGR
                     ATWAKGVAPFVTEGEKKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(18780..18953)
                     /locus_tag="IWO52_002053"
                     /pseudo
     CDS             complement(18780..18953)
                     /locus_tag="IWO52_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
BASE COUNT         5526 a         4017 c         3482 g         6175 t
ORIGIN      
        1 cacaaaattt ttcttaatac tcgaaactaa acagcttttt catcacctct tatggataaa
       61 ttcggttcaa caaacgtggg aatggaatag cttcacgtac atggtcgata ccagcaatcc
      121 aagtgactgt tcgttcaagg cctagaccaa aaccagagtg cggaacagaa ccgtatttac
      181 gtagatcaag ataccatgaa tattcttttt catccaaacc gtgattacgg atttgttcta
      241 gcaagaaatc atagtctgtt gcccgttcgc ttccgccaat aatttcaccg taaccttctg
      301 gtgcgatcat atctgcacaa attaccacgt cttctcttgt tggatgtggt ttcatgtaga
      361 atggcttgat ggcttttgga tagtttagga taaagacagg ctgttcgaat gaattcgcaa
      421 tgaacgtttc atgaggtgaa ccaaagtctt cgccccattc gatgtcatca aatccatttt
      481 tcttcagtaa ttcgattgca tcatcgtaag taatacgagg gaatggcaat tttgtataag
      541 tttttaagac ttctttgtct cgtcctagta catctaatgc atattcgcaa tgatctaaaa
      601 cattttgtac taagaatgct acatattgtt cctgtacttc taaactttct tcttgatgag
      661 tgaaagccat ttctggttcg atcatccaga attcgatcaa atgacgtcgt gtttttgatt
      721 tttcagcacg gaaagtcgga ccgaatgaaa agacttttcc gaaagccata gcagcagctt
      781 ccatgtataa ttggccactt tgagataaat aagctttttg atcaaagtaa tttgtttcga
      841 atagatccgt agtgccttct ggtgcagaac cagtcaagat cggcggatcg attttgacaa
      901 aatgatgttg attaaagaat tcatacgttg cacgaatgat ttcatttctg atctgcataa
      961 tcgcatgttg tcttgaagaa cgcagccata aatgacgatg atccatcaag aaatctgttc
     1021 cgtgttcttt tggtgtgatc ggataatcgt ggctctcgct taccacttcc acatttgtaa
     1081 ttccgatttc ataaccaaat tttgaacgac tgtcttcacg gatttctcca gttactaaca
     1141 cggatgtttc ttggtgtaga tttttagctg tttggaaaac ttcttccgac acttcgcttt
     1201 ttacaactac accttggaaa aaggctgttc cgtctcttaa ttgtaaaaat gcgatttttc
     1261 cgcttgatcg cttatttgcg acccaagcgc cgattgttac tgtttctccc acatggtttt
     1321 tcgaatcgat gatttgaatt tgttccacgt atgactctcc ttttatattc cttgaatttc
     1381 ttcaggatta attttgactt ttactctcaa taaattgctt gattctgcgt accgcttctt
     1441 ctaacgtagc gcgatcagtg gcataactca tgcggacatt ttctggtgcc ccaaagcctt
     1501 cgccagtcac aagcgctacc cctgtttctt ctaaaagatc ttctacccat tgtgtgacat
     1561 tttcatactt acacatgcgt agcgtttctt ttacattagg aaaaagatag aaagctcctt
     1621 gcggcttttc aagagaaaca cccggcagtt ctgccacaag cggatataac gtattcaggc
     1681 gttcttcaaa ggcgttccgc atttcttcta ctgtatcttg ctcacctttc aatgcttcaa
     1741 cagctgcata ttgactgact gctgtcggat tgctagttga ttgggaagca acagcgatca
     1801 ttccattgat gatcacagga tttcccaccg cataaccaat gcgccagcct gtcatcgcat
     1861 atgttttaga cacgccgttg atgatgatgg tctgattttt gattgcttct gaaatcgttg
     1921 caattggagt aaactcattg ccattataaa ctaaacggcc ataaatatca tcagcaacta
     1981 tcaaaatatc attttttact gcccattcac cgattgcctg cagttcattc ttgctataaa
     2041 tcattccagt cggattcgat ggagaattga tgattacggc ttttgttttt tctgaacgtg
     2101 cttgttctag ctgttcaaca gtaactttcc agctattgga ttcttcccct ttcacaaaga
     2161 caggaagtcc ttctgccagt tttacttgct caccgtaact gacccagtaa ggcacaggaa
     2221 tgatgacttc atcttgcggg tcaaggatcg tttggaaaag ggtatacaaa gcaaatttcg
     2281 ctccgtctgt tacgactgtt tgtgaaggtt catagacaag tccatagttt ttcttcagat
     2341 aatcaacgat tgcctgtcga agctctggga ttcctgctgt tggtgtataa tagctagctt
     2401 tcccattgcg gattgcttcg attgctgctt cttggatgtt ttctggtgtc gcaaaatccg
     2461 gttctcccac agtcaaactc agcacatctc tgccttttgc ttttaatgcc ttggcctttg
     2521 ccgcagctgc caaagtgaca gacggttcta atcgttttgt tctttttgat aactccatcc
     2581 cattcattcc tttacctttt aaattccctt gattgtcttg acttcatctc cagattcata
     2641 gccaatcagg taataattca gtttcccatt tgtttctttt cctactactt cccacacagg
     2701 ctgatcatca tacatcccta aagcagcttt ttcgatttgg atttcaggat gggccgcagc
     2761 aattgtctgt tttgcttcat tttcagtcag tccctttgtt tgatccaata cttttacttt
     2821 ttcaccagat ttaggaatga taacggcgat ttcttttcct ttatcatttg tacctgtcag
     2881 tgaaaagtat gtcttctttc gagtaaacca ataaaaatga tcggcttcct caagatttgc
     2941 atatttcttt gcgatttcta ctgcttcttt cttcgcctgc accgccggac gactggaacg
     3001 caagtaaaaa atcgagctga tcacgatgac cgctaataaa aatacgatca agccaagtaa
     3061 cacgcgatta atcgtttttt cctttgtctg ttttttcttc aagggtcgtc gctcctttat
     3121 cttcattcct tttcattata gcagatttta attatgaaaa aagacagctc ctctaacttc
     3181 cttcttcatt tttctttaag aattcatgga tatcctgata gatttcctcc atagctagct
     3241 ctttgatcgg tacttttttc ggtaatgcat ggatgatccg tgcaccatat tttgcagtaa
     3301 ctagtcttcg atccaaaata agcaatacgc cacgatctgt ctccgtcctt atcaagcgtc
     3361 ctaatgcttg gcgtaacttc aaagttgctt taggtacgga ttcctggtaa aaaggattga
     3421 tcccttctga ttctaaataa gcgttcctag ctttgaccat cgggcgaagc ggattatcga
     3481 agggtaaacg tgtgactaca acgatctgca aagcatctcc aggcaaatct acaccttccc
     3541 aaaaactgtc agcgccaaac aatacgcttt tttctgacaa aatgaagcgt ttcaaaagct
     3601 tctcccggct tccgccaaac ccttgagcta gtatttctcg tcctttttct agcatagaca
     3661 gatgcatttt ctgatagacc ttctgcaaaa catcatgaga agtgaataac acaagtattg
     3721 gccgatcttc ttgttcgatc agacctgtaa gtgctgtctc taaataatcg atatatgctt
     3781 gattgctgac ttgatggacg gcaggtgcct cttttggcac atagatcctt gcctgtttct
     3841 cataatcata aggagaggca atcactttaa gaggtgctgc ttcaatgcca agcctcttag
     3901 agagatagtg tcgattaggg ccgatcttca gcgttcctcc aatataaaga atacgatcat
     3961 atcgaggata ccaaacagtc gacggcaaca tagccgcttg aaaatcatgg agatgaaaaa
     4021 tcatctgctg cgctgtgttc ccaggaacga accaatggat caaatgatct cgccagtctt
     4081 ccagccattg atccatcagc aagtactgct tgtctaattg atcaaatagg ctatataaac
     4141 ttgctaaatc gatccgcatc tggtttgtcc agctttcttg gataatctca aaagtcagac
     4201 gcatttgtga ctgtagctgc tggatctcct gataaaaaag ctgcaggtga tcaatggctg
     4261 tcccagattc cagtggcagc tgatccaaca tctcttttgt catcatgact tcttctggat
     4321 actgtccgtt gggttgcttg ccgctcacta aactcattaa tccttctgcc atacaaatct
     4381 gtgcttcgca tagcgcagaa agttcttctc gataaatcga taaccagcgt aaggcttcat
     4441 gttctttcgg taaatagctt tgtattcggt caaacagctg atttggttct tctaattgct
     4501 ggatttgttt atggaaatag ttaatactta atcgtttgtc atttactttt tcagcgattt
     4561 ccggcaaatg atgtacttca tcgatcaaca aaaatggact agctggaagc aatggttctt
     4621 ttcgctgtgt ttcttgtact aaaaacgcat gattgacaat caaaaaatta ctttgcctga
     4681 ttttcttttg caagaaacga acaaagtctt gttcgtaaaa tgactgattt cttgctaaaa
     4741 atccaatacc tctgtgcgcg atatctgcaa agaatgaatg gttcaaattt gttaaatgta
     4801 attcatccaa atcaccggtc ttcgtttgcg tcagccaaac taagaccgcc atttgataca
     4861 aagcatattg tttttgttct ggcggttcca caagtgttcc tttaaatcgt tgcaggtcga
     4921 tatagtggcg atagcttttg ataattacag gttgtatggg ctgatctagc aattgattca
     4981 gcaatggaat gtctttttct atgatctgct gctgcaaaac tagcgaaacg gtactgatga
     5041 cagcgggttt ttcaggtgta gccaaataac tcaatggtag cagataaccg attgttttac
     5101 ccattcctgt agctgcttcg atcatcaaat cttttgattc atcttttgtg aaatggtcgt
     5161 agaccgcatt catcagtctg ttctgttctt ttcgatacat cagtgtttta ccaaacattt
     5221 tttcttttgc ttgttttttt cttggatatg tccgctcgcc ataatggacg gatgtgaata
     5281 gttccacttc ttttttctgt aaagctagcc catcgacgat ttctaacgag ggatcaagtg
     5341 gctctggttt ttctttcatt tcttcaatca catgataaat gaaacgactc gtatccatac
     5401 ctgtgtgtct acttaatcgc gcaatctgct ctaacgtgat gattggcaat tttctcatcc
     5461 gttcttcgat cagcaaaaga agttgacctg tcacttcggc atcactatct gcctgatgag
     5521 gattctcatg aataaatcca aggctttctg acaaatcagc taagcgaaag cttggttccg
     5581 tcggcaagaa aatttgtgca agttctactg tatcgatgcc tggtatcttc aaactaggaa
     5641 gtccgcatcg catgagttca tggttcaaaa aattataatc aaaataaata ttatgcgcga
     5701 caaatatggt gtctgccaaa agattgtaaa tcgtttgggc gacatcttcg aaataaggcg
     5761 ctttttggac gcgttgattc gtgatccctg ttaaatgctg gatttgtttt gaaatcttcc
     5821 gccctggatg aatatcgatc gaaaaacgtg aagtgatccg acctgattca atcatgacgc
     5881 agccaaattg gatgatccga tcttcttttg gattcgtccc agtcgtttcg atatcaacga
     5941 ctgcataagt tcttggtttc aaaatatctt cctcttctcc tgcctctaat gttcttttga
     6001 aattatacta caaagtgcag aaaaatactc tcatcgcttt ttaaagaaaa tagacaaaaa
     6061 cgcccagaca cgcaaatgtc tgggcgttta ttttttagta taggttaatt aagctttagt
     6121 aacgtttgtt gcttgaggtc cgcgttggcc ttcttcaaca tcgaaagtta ctgcttggcc
     6181 ttcttctaaa gttttgaatc cgtcgccttg gatagctgag aaatgaacga atacatcgtt
     6241 tccgttttct gcagtgatga atccaaatcc tttttctgag ttaaaccatt ttactgtacc
     6301 tgtttccata ataaaaatcc tccttgtgta tcaacacata ttttgcaatt acttgaatgg
     6361 tgtatatctg gaagatgttt atttgaaaca agctaccaca gattaccgaa caaaaactac
     6421 tcctctagta tatcataccg tttacaaaaa agctagagtt tttcgacttt ttttattaaa
     6481 aaaagttttc tcagcgatta cataaagcga aaaaccgaac tttttttaaa ataaacgaca
     6541 tattgttcag ttggtgtttt tttgctctga tgttcttttt taaactaaaa aaattattct
     6601 ttctgactac ttatcggttc tgtttcaacg gtcgaggctg gggaagattc tttagtaggt
     6661 ctgatgttct catggtattt ctgatagcct atgacagtta ccagaagaac aaggcaaacc
     6721 aatacgatca acgagattag tttttctttt ttgctcatat ctatacctca taaataacag
     6781 gatgatgtcc atcatatttc tgagtatgag taaaagcaaa agctgcagct gtagaatgta
     6841 agatatgcca gcaactttca taatcacagg tcggacagta ccaaaagtaa ccaaatcttg
     6901 gctgcaaaga ccgttcttct gtttcaggaa tcgcagaatc aagatctgtt gtctgtacat
     6961 aaacattttt gtacagacgt gtgcctgtaa taggagaccg ctgtgtttct tcagcaaaaa
     7021 ctggctgact tgttaaactt agggatagaa acagcaaaca ccataacagt ctttttttca
     7081 taaagacctc cttatcgata agtttttccc aattaaaatc atatctcaag aacgcccgtt
     7141 attcacattt tgttcataaa tgatgttttt tgtatcataa tcgtcaaaaa gaggtcgtga
     7201 ccacgtcaca acctcttttt taaacggtgt tccagaagta gcttcttcgg aaataagccg
     7261 aaatttcaca aaaatttgag aaataatttt tgtcaatctc ttcttatttc ttgaagctga
     7321 ccacttctgt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
     7381 gcccaccaaa caaaaaatat gaagagctat ttttgcttgc tgttcttatt acttgatgca
     7441 gaacagcttt ttcacaacct ctcatacacg gtgtttaatc agctgacctt cggtattagc
     7501 tcaaaaaacg gaaaaatatg tgaaactatt tttccgtttg tttctctaat actcaggtca
     7561 aaacactgat ttcacaacct cttttaataa cgaagaatcg ctgtcagact cttttcatct
     7621 ggcgcatctt tttgatccaa aataaacact ttcccgccat taatgatcac atcatctgct
     7681 aatgcattca acacttgacg ccgatcatat tctgtttcag gatcttcacc gaaaccatca
     7741 gctaagtttg acgtagcgat aaataaatgt gacacttttc cttcttttgc agctggaacg
     7801 atatcaacta actgatcgat gaatttctta tctaagagtt tattgtattc ttttgattct
     7861 tgagctgcga gttcacttga cagcttcttc gccccttctt cgatatctct aaaggatagc
     7921 tgcgcaggag aagcagaaat cgcaacgtct gcacaatagt aaggattttt ggcgattttt
     7981 ttgaacatcg tctgattttc tggtagagca aataaataaa tcggccattg ttctggattt
     8041 tcgaaagaat ccttcaagta cgtatcaact gcttggtaat agttgaccca atcgatctct
     8101 acttcttcat cttttggatt tacgccatgg aaagttactc catctcgggc tccgcctcct
     8161 ccattagaag agaagttcaa accgccacct gtcagttcag atcccaatgc agtaacgaca
     8221 tcaaccggtg ccccttctgg tagagatact tcttttactt gtttgttgct gacttcatac
     8281 aatttcatgg agtcgcggtt caatcccaat aaataataag aatagttgaa ctgcccattt
     8341 ttgataatag ccagtaaata aggaagatca cttacatagt attgatcgtc taccgggata
     8401 ctcaaacgat ggatataggt ttggtcagca gttataatga ccgctacact cgtagttgca
     8461 ctacgccaaa acgaaggatc tcctaacaaa ttttcaattt tttcttggaa cggggaccag
     8521 tcatgttcgg aatatttttt ctcaaaacgt ttctttgctt ctttcgaaaa atttttgaat
     8581 ttgatctgat ctttttccac atcttgatgg gcgacatgcg tattcagcat gatcgtgacg
     8641 aatggtcctt tagcgccttc tgaaatcaat tgtgacaata attctttatc cttttttgcc
     8701 ataaacgtac ccctcctaaa aaaatcattc ctttgtttct atctaaagtc taccacaaca
     8761 ctatttttcc atacaataaa acgtatctaa aaaaataaaa cggtttctac ggttttaaat
     8821 ttagctattt ttttaagtta tcttcataat ttcctcaatt atttagcccc agtcttcaac
     8881 tattcttcat gaaatgctga taaactaaga aaaagaataa aagaggtgta tcttatgcat
     8941 ttgattatga ttttcagttt attaagtatt atcagtttat ttgctttcag cgcttcccgc
     9001 ttattagagg cggaaacaca gcctgttcct ataaaagtac gaaaaaatga ccgctaacct
     9061 tttcataacg cgcgttctta tcctaaagtt tttttcagaa acattgaaac tctcttcctt
     9121 ctctgataaa attttaattt atagcgaaac aaattaaata caaggagatc ggatatgtcc
     9181 ataaaaaaaa cacgtctagt cctattattg gctagtctca ttctttgcct tgcttctttt
     9241 ctaccagctg ttgctgtgca agctgaagat acgttcaaag taaatgcaaa agcagctttt
     9301 gctgtggatg cgcaaagcgg taagattctt tatgatcaag atggagaaaa aaccatgggg
     9361 atcgcttcaa tcacaaaaat catcggtctt tacatcgtcc ttgatcaagt aaaagaagga
     9421 aaactatcat gggatgataa agtttcgatt tcagattatg cagaaaattt gagtatcacc
     9481 ccagatttat cgaatgttcc tttgcataaa gaaaacacct acactgtcaa agaattattc
     9541 gactctgcaa tcatccagtc agccaatgct tcaatggtgg ccttggcaga aaaagtctct
     9601 ggcagcgaag ctaaattcac tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa
     9661 gatgcaacga tcgttaatgc atcaggattg aacaactctt atcttggcga aaaccgacca
     9721 gaaggcactg gagaaaacga cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc
     9781 cgccatttga tcttggattt cccagaaata ctggatgtat caagtactac gacccaaatg
     9841 tttggcgaaa atacgcaatc accagtggaa atggtcaact ggaactggat gctcccagga
     9901 ttcgttaatt acaaggaagg cgtggacgga ttaaaaactg gtacgactga gcttgcaggt
     9961 gcttgtttcg ttggtacgat cactaaaaac gatcaacgga tcatcactgt cgtattgaat
    10021 gcggacggcc atgcggaaaa cccgagtgcc cgcttcaatg aaacagaccg tttgatggac
    10081 tactgctatg acaattggtc agaaaaagag ctcggaaaag ctaacgcaag tattcctagc
    10141 ctaaaaacca ttgatgtaaa agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc
    10201 cctgtaaaag tatgggtacg aaacgatatg gatactggca aattaacgat cacgccaaca
    10261 cttgacaaga agcaagtcac tgacaacgag atccaagcac ctatggataa aggtacaaaa
    10321 atcggtaccg ctacgatcac cttggcagac gataaattag gttatttaga agataacaat
    10381 gcgccatcga cagagatcat tacggataaa acagtggaaa aagcgaatat gttcgtgatt
    10441 ggctggcgac gcgtcgcaga atttttcggc aacttgtttt aattgaacaa taagaaaagc
    10501 atctttccat cggaaagatg tttttttgct ttttctagtt taataacagc ttttttcatc
    10561 tattttcgtt atactgtata agaaaaagga gttgaattta tgaatctaac cagagatctg
    10621 atctttttag ttctctatct tattggggta ttctctatcg tttattggcg aaaacagaag
    10681 aaggatcctt ccttgttctt ttacggcagt acgtggacat ttacggtaat cttaacttat
    10741 tttgtcgtgt tggaaatttt ttattcgacc ttttattttt tgattccatt gttgttcttt
    10801 agtatctttg ctttttctta tttcacagaa aaacgaaggc tgctgaatgg tcttttattc
    10861 aatgttttcc tcatcagttt cggcatctac ttgtttgtct tgctgtatga aacgcaaaat
    10921 attttccttg gcggactgat cgccttgatc acgattccat tgctacttgt ccttttattt
    10981 ggtatttatg gattgattgt ttttcttttt tggaacgggg tcacagtttt acgtagagaa
    11041 tcgcattcat tagctaatct tttaacgttg atactagcca tttttcttac actgtttctt
    11101 gtttttgact ttttcttgtt aaaatatttg ccgcaatgga taaatgcttt gtttttctgc
    11161 gttcccctga tcttgatcta tctatttatc gtattctaca actttttgac tgtctcattc
    11221 ttgtatcagt tcaaccggcc tcgatacaat caagacttca tcgttgtgtt aggcgctggg
    11281 ttgatcaatg gagaaacagt ttctcctctt ctagccaagc gaatcaataa ggcgattgct
    11341 ttttatcggg ctcaaagccg tgctacatta aatccgccca tcttattgat gtctggcggg
    11401 caaggagcag acgaaaaagt accagaggcc attgcgatga aacaatatgc catggaacaa
    11461 ggaatccctg aaagagacat tcttgtcgaa accaattcga caaccacttt ggagaacatg
    11521 ctctattcaa aggaaatcat ggatcagcaa atgaaagggc cataccgcgt gatctttagt
    11581 tccaacaatt accacatttt tagagccggt ctttatgcgt gtcaagccaa attgaaagca
    11641 aatggtattg gtgcaaaaac agctttctac tacttgccta atgcgttttt aagagaatac
    11701 atcgccatct tgatgatgaa caaaaaaaga catatgatca tttgcggatt gcttttcgca
    11761 ggctccgcat tcttatcgct gattacttta cttttctaaa aaaacaaggt gtgacaaaac
    11821 atatgtcaca ccttgttttt ttctttcatt ctcttaacta ttggcaaaaa gagtatccct
    11881 tttttgaccg ctacttgctg agcagaataa ataccgctgt tgcctgaaca cataaagctg
    11941 atcaagcaga tcataaagaa gtatactgca gcttgtgaac cgaacaattc gatccccatc
    12001 acaaagcagg caatcggtgt atttgtcgca ccagaaaata ctccgataaa gcccaatcct
    12061 gcaagaaaag gaacagaaac atggagcaaa ggcgctagtg ctgcaccaag cgttgcacca
    12121 atttcaaaca atggtgtcac ttctccccct tgatatcccg cgcctaaaga caaaactgtg
    12181 aagaataact tcccaataaa atcatagaca tgtgcctgtc cattaaatga ttcttgaagt
    12241 aacggaagac tcaatccgag ataacgttgt gtttgcaaaa tcaatgctgc tgccacaact
    12301 acagctccac ctatcgtatt tcgccaaaca ggatgaggaa tccattgtgc atatcgagct
    12361 tttaaaaaga cgatcgaacg gctgaatacc cagccaatca aaccaaaagc aatcccagca
    12421 acaaaaactt tgaagaacag caacatactc cattcaggaa tcacacccat ttggtaatgt
    12481 agatgagaaa caccatagct ttctgtcatg aaatttgcaa acagtgcagc aaagaagcta
    12541 gggaagatcg cttctgttcg gacttttccg attgccaaca cttctaatcc aaataatgtt
    12601 ccagctaaag gtgtgccaaa tacagaacta aaacccgcac tgattccact gataatcaag
    12661 atttcccgtt ctgatttatc caatcggaac aaacgtgctg tattatctgc taacgctccc
    12721 cccatttgga ctgctgttcc ttcacggcca acagatcccc caaataaatg ggtagttatg
    12781 gttccaaaca gtgtaagtgg aataagtcgc aacgggattt tttcttctcc accatttcct
    12841 tgatcgatca ctaagttgtt tccacgacta gagagaccgc cgtgatacac atagagataa
    12901 gcaaaaagcg caccactaac tggtagtaga taaaggagcc aaggatgtga tagtcgggta
    12961 tctgtcacaa ctgtcaagct atttaagaaa aaagcagata agctccccat gaatataccg
    13021 agaatcgctg tgatcaccag ccacttcagc atatacacta aaatgcgcat tggactcggt
    13081 aattgttttt ccataaaaaa ttcctcctca ataaaaaaac gcctactaaa gaaaggggct
    13141 gtccccagac tttagtaggc gtcattagct atctttcgtt agcatttaaa ggcgaacacc
    13201 atcacctatt cacttccatt aaaagatagc atatttttct gtaaaaaaca ataaaattat
    13261 cgttctttct ttacacgttc agcaaattca tagaaaggag ccaatttcgt taactgttga
    13321 ttacattggt cactatcatg acaaatataa ttcccctttt tcgtatacgt gccatcagaa
    13381 cctgctttcg ttgtagaaag aaaaagcgaa acattcgatg ttttctgaca gattgcacag
    13441 attcccttat ttatcgttgg tgataaagta ccactaatcc cttttaattt ttcatcttcg
    13501 taatagacca tgaatttttt ctgcgtacct ggatcattcc agccgatgta cgtatgttct
    13561 tgcaagtcga tttcattcca agctggttgt ttgagttttt tcacttttcg gaataatttc
    13621 tcgatttgtg gtttgctagg ctgcataaac ggctgaacga tttgcttcag ctcctccaat
    13681 gctctttctg cttgcgcttt tgtcagtttt acgtctaaaa ctgtcgttaa aaatgtatcg
    13741 atttctagca gttcgtgatc taatatgtct cgaatctttt cagctgctag tgcctgaact
    13801 gtttgtaccg tttttggatc attgacagat gagtacgcat ttaataactg ttctacttct
    13861 ctttttataa aaaaatattg gtatggtttt attgtttgtt ccatgatttt aaagtccttt
    13921 cttctaacca aagtgcatac tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc
    13981 tgaagatccc ggactctttt aaataagata cgttttgcat cttatcatca ccccctttaa
    14041 tgcttattct tctatgtaca tgattcattc gataaataaa agtaaaccgt atcttgaaca
    14101 gatagtcaac tgcaaacaga aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt
    14161 tcgaatgaat ggtgttcaaa agcttgcatc tgcggtaata agccgaaact tcacaaaaat
    14221 ttgagaagta attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa
    14281 cctcaatatg gtctattatt ggttttacct gttaaatcga ggattctttc ccaaagtaac
    14341 gttgccatct cttacgcata cttgcagaca tgggctgagc cgcttctttg actgcgcagt
    14401 atttgtccac gaatgtcacg atatatcctt ctttgtactt aggcggtgcg atcgttgcac
    14461 cccacatgtg cttcaaaatg atatctcgct caagatccga caaatcggtg attttttctg
    14521 cattctttac agcaattctt ggatggatat acgcatgtgt tccctcgtca aatttcgtcg
    14581 tacgccaatc atagtagaac agatcatgca aaagtccagc acgcgctgtt gctttagcat
    14641 tgccacccca tttttttgcg agcagataac tatagtaaga aacactgatg gaatgctcca
    14701 aacgagtgga atgaacatgc tgtgtgtaat tcgctaattt tttcactgct tctgtttcta
    14761 aaagatcttg tacataagac atatattctt cgtcttcacg ccatttttct gtcataatta
    14821 tcgatctgcc acctttttga atttcatttt cttctgaaat tgatctaagt ataccaccag
    14881 tgttcggatt attctagtcg ctctagataa aatagctaaa gcttaatagt tccttaatca
    14941 taaagaaaaa tgataaagaa cgatccctgc tgcaactccg acattcaatg attccgcatt
    15001 tcctttcatt gggatatata cgttctgatc agtcaaagac aagatttcct gacttactcc
    15061 ctgaccttcg tttcccatga ttatagcgaa atcctctgtt ttttctactt tgtctaatgc
    15121 atgtgcttct tcatttaatt ctgtaccgta caccgtaaac cctttgcctt tcattacagg
    15181 gattacatct gccaaatctt tttgtaacac aggcaaatga taattgcttc cctgcatgct
    15241 tcgcagcacc tttgtactat aaatatctgc cgttccttta cctaataaaa caccagccat
    15301 accaaacgca tctgccgtac ggatcatcgt tcccacattt ccaggatcct gtacattatc
    15361 tagtaaaagc cagcctcctg aaaaatctgg cgtatcttca gatgttaacg tcatcactgc
    15421 taagattcct tgcggtgtag gtagatctga catagcggtc atgacctcat ctgaaactag
    15481 aaaagtagaa atctctttgt ttacagccag ccagtctccc cattctttca tacctctctg
    15541 tgtcacaaaa agttctttta tttctgcatc agacgacgca gcttcttcga tcagatggaa
    15601 cccttccaaa agatattctg ccttttcttc tctgtatttc tttttatgta gtttttttaa
    15661 ttctttgatt cttggatttt ttgctgattg gatctctttc acattcctca cctcaactga
    15721 ttatatcatg gaataggtgc ttgagcttgt ttcatcctaa aaaaatcggt atcattgatt
    15781 tataaaacag gaaagaaagg tgacgaacat gcgcaaagtt aaaatgaatg tccaaggacg
    15841 tgtccagggc gtgggatttc gctatatgac caagatggtt gctgatcagt taggcgtgac
    15901 cggaacggta aaaaacgaag acgacggctc agtcacaatc gaagcaatcg gaaatgacga
    15961 catcatccaa aaatttatcg aagaagtcaa gaagtctccg agcccaagtg gacgagtgca
    16021 gtatgtggat gtccaagaag atccgatgat tgaggaacga aagaaatttg atgtggtggg
    16081 ataaggttgt ttttgaagcg ctttgcctcg agtattaagg agaaacattt tcaaaatagc
    16141 ttctcatatt ttgaaatgta atgacttaat accgagaggc agctgataaa acaccgtcaa
    16201 taaggttgcg aaaaagctga tctgcatcaa gcattaagga ctggttgcga aaaagtcgct
    16261 tagttttgag tattaaggaa aattttgtga aaatggcttt ccacattttt gcataaattt
    16321 gtcttaatac caagaaactg acttttgaac accgtcgata aggttgtttt tgaggcgctt
    16381 tgcctcaagt attaaagaca ggtagtaaaa aagctgatct atcacttgca gtgagttcat
    16441 ttgactcctc tatttcgggc agaataaaaa agtttttagt tttttaaata agaaagaaac
    16501 acttggaatc actcttttct ttccaagtat ttctttcttg attttttgtc ttttttcaaa
    16561 atttttcgct acgaaaaaga aagaatattt atatgaaatt tctatgaagt tgattgaatt
    16621 ccatgcgctt ttttagtata gttaagtttg tgtaaaaatt atagaagagg aagtattcta
    16681 tgaataaatg gaaaaactgg ttattgggat ccggacttgt cagtatcctg ctctttttat
    16741 ctggctgtgt gcggatggat tcaaacggaa accctgacac ttcaggaatc gtttatcgag
    16801 tactcgtcca tcctatgggc caagcgatta catatctagt tgagaatttc aattggtctt
    16861 acggctgggc agttattgca atgactgtga tcgttcgtat catcatcttg ccactaggta
    16921 tcagtcaatc gaaaaaaaca atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag
    16981 ttgaagcagc acaacaaaag ctaaaagctg caacaacacg tgaagaacaa atggcagcac
    17041 aagcagaaat gcaacaagta tatcgcgaaa acggattgag tatgaccggc ggaatcggct
    17101 gtctgcctct attgatccaa atgccgatct tctctgcctt gtattttacg gctcgctaca
    17161 cagaaggtat ccgtgaatca tctttttacg gcatcgatct aggctcacca agtatggtgc
    17221 ttgttgccat tgcaggggtc gcttatctgc ttcaaggata tatttcaaca atcggtattc
    17281 ctgaagaaca gaaaaagacg atgcgtacga tgctgatcgt ctctcctgct atgatcgtct
    17341 tcatgtcgat cagtgcgcca gccggtgtta cactttactg ggtagtcggt ggtgtgttca
    17401 gctgtctgca aacattcatc acaaacgtta tgatgaaacc acgtttgaaa gcacaagtgg
    17461 cagaagagtt gaaacaaaac ccgccaaaac aagtcgtcac tccaagaaaa gacgtgacag
    17521 aatcagcaaa gccagctgac aacaaacaag caacaaaaca aatcaaacaa ccaaaaaaca
    17581 gtacaaacaa caaaggccgc aacgcaggaa agcagcagaa gcggaagtag atagtaaata
    17641 agagtgtaat aaaaattcat taacagtttt agcaattaaa aatatcgacc aagatttttg
    17701 aaaaagaaat ctggtcgata tttttattta agtgaatgtt taatttgaat catcgattga
    17761 tcgctatcta attgcagcag catcttttta tgaagaaaca actttttctt atctatcaac
    17821 gtagacaggt gttgctgttt cttttaataa tgtacttaat tccttaatat tttgttttcc
    17881 ttcttctaac atccaactac tcgctttttt ttcgccttcg gtaacaaaag gtgccactcc
    17941 cttcgcccat gttgctcgcc cacataagat accattgaat gtcgaacctg attgttttgc
    18001 aaaacgtaat gtatcttgaa atagcgtagc attgactccc gcacttaaaa aaataaacgg
    18061 ttgttcagtc actttcgatt gttcatagaa atacgcggat gcctctgctt gactataaca
    18121 agtctcttgc ccatatcctt ccacaaatct catattcact ggcacttcta ctttcaatac
    18181 atccacttga taacgttctt ttgaaaattc tcgcatggct tcaataactt tatggggttt
    18241 tacttttgca taggcaagag aagacggatc gccaatagtt gtatcataag aaaggatctc
    18301 taaaaagaaa ggtatgcctt ctgcttcgca ttctgaacca atccgttcta taactatttg
    18361 tttttgcaca ttaatctctg ctggttcatc cacgtcatag tacaataaaa atttacaagc
    18421 atccgcacca gcttctttta accttttcac cgaccaaata ttcaataaat caggtaatct
    18481 tcccggcatt ttcgtatcat agccggtttt ttcataagct agcagcaagc cacattcttt
    18541 ctctcgaagc gctgctgcag gcaggccgta ttctggatca agtaaaatag ctgaggcata
    18601 gggagtcagg tattgagaaa ccatctgttt aaattggatg atgtcttctg atgtagcttc
    18661 tttatattgg ttgatcattt tcttcaaagc accacgctgg tcgattgcta acgcgctaat
    18721 aattccagct tcatccgtca atcgcttcaa acgttcctgc ttttctatac tcatcttctt
    18781 catatatctt cacctatttc tttagttaat attgtggcta tttttgtttt tgattttgct
    18841 tttctcaact gttctgtaac ctcttttcgc atcaatgatc gtgcaacgag agacaataat
    18901 ttcaaatgag tcgttcctgc ttctgaatct ggaataaata acgcaaaaat aaaatagtaa
    18961 atatctactc gtgaaaacgg gtggtttttg gcagccctag aaggcctcat cacttgctta
    19021 tcctcttaag aggggctttt taagggacca ccaacttgta ccgaccccca aaagttagac
    19081 caaaaaatct aacttttgga ggtcggtatt ttttatggct aagtatactt ttgaacttaa
    19141 attaaagatc gttcatgatt atttggatgg caaaggcgga agtgattatc tggcaaagaa
//
LOCUS       DACEDO010000057        19012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEDO010000057 DACEDO010000000
VERSION     DACEDO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.057"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            381..1043
                     /locus_tag="IWO52_002054"
     CDS             381..1043
                     /locus_tag="IWO52_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356311.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1122..4349
                     /gene="fss3"
                     /locus_tag="IWO52_002055"
     CDS             1122..4349
                     /gene="fss3"
                     /locus_tag="IWO52_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0356312.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            4463..4777
                     /locus_tag="IWO52_002056"
     CDS             4463..4777
                     /locus_tag="IWO52_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0356313.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            4790..5164
                     /locus_tag="IWO52_002057"
     CDS             4790..5164
                     /locus_tag="IWO52_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0356314.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5174..5518
                     /locus_tag="IWO52_002058"
     CDS             5174..5518
                     /locus_tag="IWO52_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0356315.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            5589..6941
                     /locus_tag="IWO52_002059"
     CDS             5589..6941
                     /locus_tag="IWO52_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0356316.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7001..8143
                     /locus_tag="IWO52_002060"
     CDS             7001..8143
                     /locus_tag="IWO52_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ0356317.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8168..8422
                     /locus_tag="IWO52_002061"
     CDS             8168..8422
                     /locus_tag="IWO52_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356318.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            8678..9862
                     /locus_tag="IWO52_002062"
     CDS             8678..9862
                     /locus_tag="IWO52_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0356319.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            9877..9996
                     /locus_tag="IWO52_002063"
     CDS             9877..9996
                     /locus_tag="IWO52_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0356320.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            10001..10222
                     /locus_tag="IWO52_002064"
     CDS             10001..10222
                     /locus_tag="IWO52_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356321.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10235..10735
                     /locus_tag="IWO52_002065"
     CDS             10235..10735
                     /locus_tag="IWO52_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0356322.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            10832..12679
                     /locus_tag="IWO52_002066"
     CDS             10832..12679
                     /locus_tag="IWO52_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ0356323.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            12682..13578
                     /locus_tag="IWO52_002067"
     CDS             12682..13578
                     /locus_tag="IWO52_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0356324.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            13628..14017
                     /locus_tag="IWO52_002068"
     CDS             13628..14017
                     /locus_tag="IWO52_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0356325.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14004..15506
                     /locus_tag="IWO52_002069"
                     /pseudo
     CDS             14004..15506
                     /locus_tag="IWO52_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            16251..18077
                     /gene="ltrA"
                     /locus_tag="IWO52_002070"
     CDS             16251..18077
                     /gene="ltrA"
                     /locus_tag="IWO52_002070"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0356326.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18211..>19012
                     /locus_tag="IWO52_002071"
     CDS             18211..>19012
                     /locus_tag="IWO52_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0356327.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6432 a         3115 c         4204 g         5261 t
ORIGIN      
        1 gaagataaac acggaaaact tccttggaaa tctgaaattt gtgaaaagtc cttgtatatc
       61 aagggctttt tcttttttct ctatcacagt tttaagccat ttttggctgt tttggaccat
      121 ttttcaatca tattccctta aaaattgtcc tagattgtcc gacattgact atcagtgtcg
      181 tcaaaatagg gcaaaattta gtagtcagct tgacgacaaa tataatacga aaaataccaa
      241 aacatttacc gaatttagca acttttgaac ccaagtttct caaagggtca gctattagca
      301 aaaaagacaa aacatgtact tttctactaa aaagttgatg tgataggctt ctatctaaat
      361 aactattagg agtttttatc gtgaagcaaa atgatggacg tatcatttgg aaaaatcaaa
      421 gtgagttaaa actgatttta acaattaatg aattcatcga aaaacacgga attacatcta
      481 gccgtcaata ccagaaaaag ctatcagaaa atccaaattc tgcaccaagt atgtggttca
      541 ttaacaaaaa atatggatca tgggagaatt tattgattag tataggacga gaaaatactg
      601 ggtatggaaa atgggctaga atgtcagaac aagaattatt ggaaatagta gaagccttta
      661 ttaagtgcga gaagataacc tctcaacgaa tgtacgaaca aaaatcagta gggaagaata
      721 ttccttcact tagtaccatt aaaaagatgt taggagatat acgtccactt tttaaagaaa
      781 aaaatgatgg atctcgattt actgatttcg aactgctgtt agaactaaaa aatgaaataa
      841 tccgattaaa actacaggat gatttatcaa tgacaaagtt tcgaaaacta gttcaatccc
      901 caaaactacc atcagttgat acaattatga aaagaacgaa caaaaattgg gaggaattga
      961 tggcagagat tggatttgat tatcgaagaa tcaaaattta taagcagaga aacaacctat
     1021 ccaagacaaa gaaaactaaa tagtatcact ctaaatcaga cgttcaaaat taaaatttgg
     1081 acgtcttttt tattacccaa aaacgaaagg agatttattt tatgaaaaag ccaagattta
     1141 agaattggcg tttgctcgca acactgagtc tattatgcca aacaattggt ggatcgcttg
     1201 gcccaacgat tgcctttgct gatgaaatta ctcatccaca aacggtaact gtggaattgg
     1261 atttggctca ccagtatgct gtcgaaggta catttagcga tggtcgtccc atgtcagaag
     1321 ttaccgttcc gcactatgca gtttacaatg gtgtgaagca agatattttc tgtattgagc
     1381 caggggtgcc gatttacaat gagttcactc ctggttatga gaaaaatcca ttgcctgata
     1441 tgtcggagaa agcgaaatta gtttccgttt tatggaagga agctggtacg gatgtggata
     1501 cacatattgt ggcccagaag atgatttggc aagaagttaa tggctacacg cttcactcaa
     1561 ttaaacgttc tgatggtagt gcagtaaata tcgcagccat tgagtcaaaa atcaatcaag
     1621 ccattgcgga ttatcaaaag aaaccaagct tccataactc cacagtgaaa accgtgttag
     1681 gtcaatcgac tactgtgaca gatacgaacg gcttgaattt atcggagttt gatgaagttg
     1741 tcgagaatat cgcaaatatt gattatcgtg tgaacggcaa tcaactagtg attactccaa
     1801 atgccaattc aaaagagagt ggtgaattaa ccctaaagaa gtcagctaat acaggaacac
     1861 cggtagctta taaaatggct ggtcaacaaa ctttgatggc gggggcaatt gataagccca
     1921 atacctacac aatcaaaatt gatgtggaaa ctgagggttc tttgaagatt aaaaaagtcg
     1981 ataaagaaac aggaaatgtt gtaccgggaa ccgttttcca tttagacttt ggaaaaacat
     2041 tacctgcaaa agacgtgacc accgataaag aaggcattgc gacattggat gagattcctc
     2101 atggcgcgaa agtaaccatt actgaaaaat cagtgccagc tccttatacg attgacacta
     2161 caccaatcac cgcaaccatt aaatctggtg agactatttc tgtgacttca aaaaatactc
     2221 gcgaaaaagg tcaaatcatt ttgaataaaa tgggggtgga aacaggaact gatctttgga
     2281 acgacaaata ctcattagcc ggaaatacgt ttgccattcg caaagacagt ccaaccggtg
     2341 aaattgtcca agaaatgacc acggatgaaa aaggtcatgc ggaaacacca aaagagattg
     2401 ccaatgcgtt ggaattggga acctattatg tgaccgaaac caaagctagt aatggctttg
     2461 tgaatacctt taaaccagta aaagtcgaat tgaactatgt caatcaaacc gtggctcttg
     2521 ttaccagtaa cgtaaaaggg caaaaccaag aaatcactgg tgaaaccact ttgacaaaag
     2581 aagacaaaga taccggtaat gaaactcaag gtaaagctga gtttaaagga gctgaatata
     2641 ctctctttac tgcaaaagat ggtcaagcgg tcaaatggag tgaggctttt aaaccagagt
     2701 tagtgaaggg aacgaaagct tctgatgaaa cggtgacttt gactttagat gaaaagaatc
     2761 aagttgctgt taaacactta gcgattaatg agtattactg gcaagaaacc aaagcacctg
     2821 aaggatatac cttggatgaa acaaagtacc ctgtttccat caaaaaggtg gatgataacg
     2881 aaaaaaatgc cgtgattacc cgagatgtta cggcaaaaga acaagtcatt cgctttggct
     2941 ttgatttctt taaatttgct ggatcagctg ctgggactgc cgaaactggc ttcaatgatc
     3001 tgacctttaa agtatcgcca ttggaaggaa ccagtgagat tacagatgcc gaagatgaag
     3061 caattacagc ttataacgag caactaggtt ttgatggcta tggcaagttt gaaaatcttc
     3121 cttatgggga ttatttactt gaagaagtag aggctccaga aggatttcaa aaaattacgc
     3181 cattagaaat ccgttctact tttaaagaaa ataaagcgga ttacactaag agtgaataca
     3241 tttttacgat taccgaaaaa ggacaaaaac aaccaattaa aacggtgacc gttccttacg
     3301 agaaactgac gaataaagca ttttctgtta gcttgaatcg tttgatgctc tatgatttac
     3361 ctgaagaaga agatagctta acttctcttg ggacttggaa agacggaggc aaagaattga
     3421 cagaccttga ttctaccgag ctagttgata aattgagcta taacttgcat gaaatcaaag
     3481 aagactggta tgtggtagcg caagccattg atgtggaagc gacaaaagct gcccaagaaa
     3541 aagatgaaaa agccaaacca gtggtaattg ccgaaacaac cgcaacgttg gcgaataaag
     3601 agaaaacagg aacttggaaa attttgcata aattaaccgc agaacaagtt ttggataaaa
     3661 ccatcgtctt attcaactat gtgtatgaaa acaagaaacc ctatgaagca ggtgatgatc
     3721 cagtagcgaa agatgctagc ttgaacaatc aagcccaaac tgtcaattgt acggtggaac
     3781 gccatgtttc catccaaaca aaagcccacc tagaagatgg ttcgcaaacc tttactcatg
     3841 gggatgtggt ggatatgttc gatgatgtgt cgattaccca tgatgtactg gatggctcaa
     3901 aagaagcttt tgaaacaatt ctgtatgcat tacttccgga taatacgacc aaagaaattt
     3961 ggaaatctgg caaaattgat tatgaagtga atgacaaaga attcactaaa accgtacttg
     4021 cggaaaaagt ggacacaagt aaatatccag aaggtacaag ttttaccttc aaagaaatta
     4081 actatggcaa agacggaaac atcaatggga aacacaatga ggatctaaaa gaaaaaactc
     4141 aaactttaac acctaaaaaa gttccgacaa caccaagtgc accagaacaa ccggaaatac
     4201 caactgttcc aagtgactct caagaatcta gtcctacagt aaagacattc ccacaaacag
     4261 gggagaaaaa ttctaatgtt ctgctgttta ttgggtttac cttgatcttt gcgactgctg
     4321 ggtattattt ctggaatcgc cgaaactaag gtgatgtgat gaaacaacta aataggaaaa
     4381 accaacgtgc gcgaccgcct cctaaaaaaa gtggtcgcgc tatttttgta tccaaaacta
     4441 aaaatgaaaa gaggaaaaga agatggaatt gaaatttgtt gtgccagata tggccgaaac
     4501 ctttggaaag ataaggtatg ccggcgaagg cgaagtttta actgaaggat atgggcggaa
     4561 tactacagta attggtcgta gttatcattt gtattccagt aagcaacgag ccgatgatat
     4621 tgaagtggtg gtagctgcgg aagccggcga aaaggatttt gatcaagatc aaccactaaa
     4681 agccgtgaat ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttaccga
     4741 ctacgtgttg tatgtcgatg atttagtgaa agcataggag gaaaagaaga tgagattaac
     4801 agaaggtatt gttgtagatt caggactaac gtttgggaaa ttacgatttt ccgcattacg
     4861 ccgagaagta cggaagcaaa atgaggatgg aacggttagt aacgaggtaa aagaacggac
     4921 ttataatttg aaatcctctg cgcaaggtcg aatgatccaa gttagtatcc cagcaaatgt
     4981 acccttacgc gagtttgcct atgacgcaga ggtagagttg gtcaatccaa tcatggatac
     5041 ggtcgccaat tatgttttcc gagaaggaac tactgtcaat tggttcatta aggccgatga
     5101 tttagtcttg aaacgacaac cagatcaggg aaattctaca aaccaaaatg aaggaaagaa
     5161 ataggtgaag cgtatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg
     5221 aaaaggtgag ctaacggagg aacaactcac taaagcatta acctatgatg aaaaaatgtt
     5281 tctgattatg catcaagggt tactggaagt aggtaacgat gccgaagaag attttgatgt
     5341 actgaattgg ttaggggaag aagagtcctt ctttatggtt atcgaagtaa atgaaagtct
     5401 atgtcgagaa gccgaaaccg tattggaaga aattggtgtg gaaatgccgg atgccattga
     5461 agttttttta aagcaactag tggaaacgaa acaactccct atagcagtaa atgactagca
     5521 taactgcttg aaattggatc aatgaaatcc tatgaagctt ttttcgattg gaggtggaaa
     5581 ccataaaaat gtacaaaggg catcgtatac gggcaggaga tcagcacttg gtttatcact
     5641 ttgttcttgg ctggctactc gctctgttca tcggttggat gagcgtcttt tattttcaag
     5701 agcttagaca atttgatatt tctaagctat cactttctac tatagaaatc gttcgttcta
     5761 taaaagattt aatctatttg ctaggaagtc tcgttctttc aggaagcacg atgttgcttt
     5821 atatacactt ttttcaggat cattggcgca gtttgtggca tcgacaaaag ctagcccgga
     5881 tgattctaga aaatcattgg tacgaagtaa aacaaactca gagtgaagga tttttcaaag
     5941 acctcaatag tagtcgaacc aaagagacaa tcagttactt ccccaaaatc tattaccgta
     6001 tgaaggatgg tttactttct attcgcgttc aaatttcact gggaaaatat caagatcagc
     6061 ttttaaagct ggaaaagaag ttagaaagtg gtctatattg tgaactagta gaaaaggaac
     6121 tcaaagactc ctatgtggag tacactttac tgtatgatat aattgccaat cgaattggga
     6181 tagacgaagt agtggcagaa agtggggctt tgcgattgat gaaaaatcaa gtatgggctt
     6241 atgattcttt acctcatatg ttaatcgctg gcggcacagg tggtgggaag acttactttt
     6301 tactcactat catcgaggct ttattaaaat ctgatgccga gctttttgtt cttgatccca
     6361 aaaatgcgga cttagccgat ttaggtacgg taatgcctca tgtctattcc caaaaggagg
     6421 aaatttctgc ttgtgtggaa gatttctatg agcgcatgat gactcgtagc aaggcaatga
     6481 aagaaatgcc caactacaaa acaggagaga attatgcgtt tcttggactt ccaccaaact
     6541 ttttaatctt tgatgaatac gtggtttata tggaaatgtt aacgacgaag gaaagtgcgg
     6601 tgattttgaa taagctcaaa caaatcgtga tgttaggtcg tcaatctggt ttctttctga
     6661 ttctagcttg tcaaagaccg gatgcgaaat atttaggaga cgggattcgc gatcaattta
     6721 atttctgggt ggctttgggt cgtatgagtg aactgggata ttccatgatg tttggcgagg
     6781 ttgataaaaa attctttatg aagcgtatca aaggtcgagg ctatgtagat acgggaggga
     6841 gtgtgattag tgagttttat acgccacttg taccaaaagg atatgatttc ttaagggaaa
     6901 ttagcgacat tggtggatta aacgttaata cggaaggata aaataatagt atgtagccta
     6961 ggagtgtcaa gaatcctggg ctttttttac gaggtgattt atgacatttt tagatttatt
     7021 cgctgggatt ggtggctttt gcttaggtat ggaacaagcg ggtcaccagt gtatcggctt
     7081 ttgtgaaatt gatgattttg ctcgccagag ttataaggca atccatgata caagtaagga
     7141 ggtggaaatg catgacatca caagtgtatc agacgagttt attcaatctc tcggaccagt
     7201 ggatatcctt tgcggtggat ttccgtgcca agctttttca attgcgggaa agcggcaagg
     7261 attttccgat actcgaggta ctttattctt tgaaatcgct cggttcgccg ctattctcca
     7321 acctaagttt ttattccttg agaacgtccg gggattactc aatcacgaag gaggggctac
     7381 gttcgagacg atcctccgaa cgctggatgg attggggtat gatgtggaat ggcaagtgct
     7441 taactcaaag gcctacgttc cccaaaaccg tgagcggatc ttccttatcg gacattctcg
     7501 agacgcatgt accgaacaag tatttcctat cattggatcg tctccaacat ctgatcaaaa
     7561 catcagaaac ttattaaata tcaacccttc caatcgagga atggggggac aagtctatgg
     7621 gtcagatgac gtagcgccta ccttgaccgg tgatgaagga atcaaaatcg cattgccggt
     7681 agatgatgga atttctgtag cgggtatgtt actgggaaat tttgaacaag gcaatcgggt
     7741 ttatgagata actggaacag cgcctacttt gtcaacgaaa caaggcggaa cgaagatcat
     7801 gcttcggaaa aacgaaacag aatatcaaga ggtgaagcca gggaacagtg tgaacctggc
     7861 ttttcctaat tccacaagtc gtcgaggccg tctgggaaaa caaagtgtgc ataccttatt
     7921 aacaggagac caacaagcag tcgtcacgga tcagtatcaa attcgaaagt taacacctag
     7981 ggagtgctgg cggcttcagg gctttccgga ctgggctttt gatcgagctt ctcaagtcaa
     8041 ttcagatagt cagttgtata agcaggctgg aaattctgta acagttccgg tcatttttga
     8101 tattgctaga agattaaagg aggtaaaaac agatgattta tgatgagttg attggggaat
     8161 ctactgggtg attggcaaga ttcagtcaga tccggaattg gaagaagaac tgtaccggct
     8221 caattttgat attcgcaaaa acggggtgaa agtccctgat gatccgtatg taattgatga
     8281 ggaaacggat gctcgaatgg agctgaatca agtcatcagt gaattggaac gaattgcgga
     8341 ctttgctaaa gaacctgata tccgccaata cttgtttgag ataaaagcag agttggaaat
     8401 tgagggtatt accgcggagt aacggccaaa taaaccactg ataggcactg accgcggggg
     8461 atttgcgaag cagaaatcct ccgtggtgct gtgcctgttt atggacggcg ttagccggcc
     8521 atgatgtctc accccccgta tctaacaggg gggtacaaaa acacaaaaaa taagcaagca
     8581 acgataagga atggctgtcc tatcaaggat aataagcaat ttgtgtagat gttaactgat
     8641 ttgaaaaagt taacacctta aatttggagg tgagaacttg gcacaaagga atttagatta
     8701 tcggctatta aaagaccggc gtaatgaata tggcgtttca caaaataaat tagctacgac
     8761 ttgtggactt agtcgcccgt acttaaatca gatagaaaat ggtggcgtaa ccgcatcgac
     8821 aaaaaccatg aggaaaattt ttgaccaact ggaaagtttc aatcccgagt tacctttaac
     8881 gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcgagga aaatcattca
     8941 agagattctc catttgaaat ttgattatat gctccacgaa gattacgcct tttactccta
     9001 tcaagaacaa tatgttatgg gagatattgt ggtgatgttg tcacatgaag aagataaagg
     9061 cgtcctttta gaattaaagg gtcgtggttg tcggcagttt gaaacttttt tactcgccca
     9121 aaagcgtagc tggtatgact ttttcgaaga ttgtctaaaa gccggtggcg tgatgaaacg
     9181 cttagacctg gcaatcaatg atcgagttgg cctgttagat attcctgatt taacgaaaaa
     9241 atgtcagaag gaagaatgta tctccttgtt tcgtaccttc aaaagctatc gttccgggga
     9301 actgttgaaa cctgatgata aagatggtat gggcaatact ttgtatattg gcagtcttaa
     9361 gagtgaggtc tatttctgtt tgtacgaaaa agactatgag caatatatca agttagggat
     9421 tcctttagac cagaccgaaa cgaaaaatcg ttttgaaatc cgactgaaaa atgatcgagc
     9481 ttatcatgca attcaagatt tgttaaaagg tcgcagtatt gaaagtacca cattttccat
     9541 tattaaccgg tacttgcgat ttgcggataa ggtggaaggc aaaaggcgaa ctaactggcc
     9601 cttgaatgaa caatgggggc gcttcattgg acggaatcag aaggaaattc aattaacttc
     9661 tgaaccgaag ccttatacca ttgaacgaac gttgaactgg cttggacgac aagtcgcccc
     9721 tacgtggaag atggccaagg agttggatcg gttgaatcag acaacgtaca ttcaggatat
     9781 ggtaaataat actcgactat cagaccggca caaaaagatt ttggagcaac aaagtatggc
     9841 aattgaaaat ttgattatat gaaaagagga atcgtgatgg aaattgtgaa agatatttta
     9901 ttggtgtttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgca tgcaagtgca
     9961 gcggcggacc gagcaatgga aaagaaagga aagtgatgaa atgaatttcg gtcaaaattt
    10021 gtatcaatgg tttttaacca atgctcaatc tttagtctta ctagcgattg tggtcattgg
    10081 tttgttttta ggatttaaac gcgagttttc caaactgatt ggcttcttgg tgattgcttt
    10141 gattgcggta ggtctggtct ttaatgcttc tggtgtgaag gatgtcttac tcaatttgtt
    10201 taaccgaatt gtcggtgcct gagggggaat cacaatggaa cagatacgtg tttatattgc
    10261 gaaccttgga aaatacaatg aaggtgagtt ggtgggggca tggtttaccc cacccgtcga
    10321 ttttgatgaa gtgaaagaac ggattggatt aaatgacgaa tatgaagagt acgcaattca
    10381 tgattatgaa ctgccttttg agattgatga gtatacacca attgaggaaa ttaatcggtt
    10441 gtgtggttta gcggaagagc ttaaaggaac gccaattggt gaggtcgctt cagagattca
    10501 acatgccttc ttcaattctt tcgaggaaat ggtagagcat gtggatgata tcatctatta
    10561 tcccgattgt aatgacatga gtgatgtagc gtattacttg attgtagaag ctggtgattt
    10621 aggcgaagta ccagaacact tgaagacgta tattgatttt gatgcgtgtg gtcgagactt
    10681 ggagattggc gggaattatt tggttacgag tcgaggggtt tttgaactaa tgtaaatcat
    10741 ttgttagaat tgaccagaaa tttctatcgt acttttactt ttctatttga tatactgaaa
    10801 tagaaaatat ttgttgatag gggtgttact aatggttggc aataagtggt acaaatgtga
    10861 tcttcatatt cattcaactg cgagtcagtg ctttcaggat aaaaaggtta ctgctaagga
    10921 atgggtagaa gaatgtttaa acaaaggact cgattgtgtt gcattaactg atcacaatac
    10981 aggggaaaat attgatgaat ataagaaagt ggctatcgaa aatggcttag atttttttcc
    11041 tggagtggaa ttaacgtgtg gagaaaatgg aactcattta ctagtcctat ttgataaaag
    11101 tgcatgtaca aatgatatct ctgatttctt aaccaagatg ggtataaaaa gagaggattg
    11161 ggccagcgct gcagcttgct caatgaaatc agttttagaa gttattgatt ttgctgagca
    11221 cgagggcttg ctcgttattc ctgcacatat agatgagttt aatggaattt cttcagcaaa
    11281 atttggaaat ctaggaaaga tatttgaatc agaaaatata tatgcagtac agacggttca
    11341 aaaagaattt tttgaaaatc gctcacaaat gattccctca tctaagcgtg acactatcta
    11401 tgatagtgtg aatgccagat atgatggtag agttggaaaa gatactttgg aatcttggta
    11461 taaatcagtt gttgaggcag aaaaaaataa tatgacattt cttagctttt ctgataaccc
    11521 acactcgaaa ggaaattcaa aacatggact ttggggaata ggcacaagat attcctatat
    11581 aaaaatgaag gacgaacctg acttgggatc acttcgcgat gctttaatgc taggcgaaac
    11641 tcgagttcat tctgattttt ctaatttctc cattaatgaa aatgaagttt tattggaaaa
    11701 attaagcttt tctgggacca ctcttagcac aaaagaagtt gtagtcgaat tcagcgataa
    11761 tttaacttca attattggag gaagaggaac aggcaaatct tgtattacaa gattcttggt
    11821 ctatgtcctt ggaaaggaag cagaacttga tcaattttct gaaatacaaa gtgattatca
    11881 gaactttgca caaattgaac ataatggctc tggaatattt ttgaaggata caattgtgaa
    11941 actgaatatt ttctataagg ggacaaaata tcaaataata agaacacagg atagacattc
    12001 tatttatgaa tttacacaag agaatggtct ggttgaaaaa gaaacagaac ggttgagaat
    12061 gatttccgat aaggtgagta tatactctca aaaacaaatt tatgaaattt caaaaaatca
    12121 aactagtata ctagaattac tagatggata taactctgat ttgatttcag agtataaaaa
    12181 tgagattgaa acatgtgtaa acgaaataag aaaattaaat tgggatattg tttctgtgaa
    12241 aaaagaaata caagataagg cgaaggtaga gcttgaaatt gaagatttaa aaaaacaagt
    12301 agaaaagcta tctcataaga gttataaaga tgtttatgat gagtattcaa aagagacaga
    12361 tatttatagg gaactgaaac gtgatgtgga ggcacttaag gaaattccca aaaatctaac
    12421 tgatagtgtt gacaaaattg actttggcaa tggtataaaa gttacagacg aaattgatga
    12481 acaccgcgga gaattgatta agaatttagt gaccaatcgg gctaagattc aaacaataat
    12541 taatgaaatg tcagatgaaa ttgatagtta tagagcagag ctgaataaat cagattggaa
    12601 agttaactat aatgaggttt caaagaggta tcaaagaggt atcaaaaact tagtgaagag
    12661 ttatctcaag atgaattaaa aatgttacaa aaccttgatt ctttatatca atctttatca
    12721 aaaactgaag agcgattgga agaaattatt caacaagaaa taaagcttaa agattaccaa
    12781 gttgaggttg actcagcaat gtcaagaatg aataaatcat atcaaaattt ctattctgag
    12841 agaagtagtt cctcgcgcga aattttttct gatattacag gaatagaggc tactattcaa
    12901 ccaaataggg atttcaataa ttatgttaaa gagctcagag cttgtattaa taaggaggac
    12961 acttttgaca gtgagtttgg atctcttatt gataagctga caaaaaattc cattactaat
    13021 caagatatat ataatgacat acttgatata agagttagtg gggaaagtag tattttttca
    13081 gataaaaaat taatcaattc tataagaaaa ttgaatgatg cacaagttct tgaattacaa
    13141 acccttaagc caaatgattt agtagttatc cgacttgaac taaatggaaa aaaagttagt
    13201 ttatcgaatg ctagtgctgg tcaaaagaca tctgcaattc tttctatgat tttggcgtat
    13261 ggaaattcac cactcatatt ggatcagcct gaagatgatt tggatagtca attaattaat
    13321 aacttaatcg taagaagtat cattacaaaa aaggaaaaca gacaaatttt aattgtaact
    13381 cataatgcaa atatcccagt taatggtgat tcagaatggt tgattagcat gggagattca
    13441 aaagaaatat cggctgatca gtgcgggtcg gtagatgaac ccaatatcaa gccaagaata
    13501 tgtactgtga tggaaggtgg cgaggatgct tttacaaata gagcgaaacg ctatggtttt
    13561 aaacaaattg tagaataaca ctagagggtg agacatatta ttatgtccgc cctttttagg
    13621 aggaaaaatg aaaaaaataa aaagctatac cagtatctgg gcagttgaaa aagtcattta
    13681 cgcaatcaat gattttcagt tgccttttcc tgtgactttt aaccaaatgg cttggttcgt
    13741 tttatcttta ctgtttgtca tcgtatttgc ccatgtgcct ccactatcta tgatagagga
    13801 ggtcttttta aaatacttag gcataccggt ggctgtcact tggtttatgt cgcaaaagac
    13861 gtttgatggc aagaaaccac tgggattttt acgatccttt atcagctatc acttgcgatt
    13921 taaggtgact tttgcaggaa agaaagtcaa agaacagaag aaacgatggg atgaacccag
    13981 tactcttgta aggagtgtga actatgtatc cgattaagta tattgaaaat aatctcgtct
    14041 ttaaccaaga aggcgagtgt tttgcctact atgaattagt gccttacaat tactcctttt
    14101 tgtcacccga acagaaattt caagtgcatg ataactttcg ccaactaatt gcgcaaaatc
    14161 gtgaaggcaa gatccatgcg ttacaaattg ccacagaaag tagtattcgc gccacacaag
    14221 aacgatcaaa aaaagaaatt accggtagac tcaaagaagt agcgaaacaa cgaattgata
    14281 tacagacaga tgcattagta tccatgattg gcgatagtca gattgattat cgctttttta
    14341 ttggctttaa actaattgcg acagatgaag aagtcaatct aaagagtctc aagaaatctt
    14401 tcttttctgg gcttcaagaa tttgtctatg gagtgaatca tcatttgatg ggcgattttg
    14461 tttccttatc caatgaagaa attcgctgct attctaagct tgaaaaatta atggaaagca
    14521 aattggctcg aagatttaaa gtcaggcgag tcacaccgag tgacctgacg tatttgattg
    14581 aacatattta tggcgaaaaa gggataccgt ttgaagaata cgaatttcag ctaccaaaga
    14641 aaaagctaaa atcagaaaca ctggtgaaac gttatgactt attacgtcca aatcgttgtt
    14701 taattgaaga aaaaccaagg tgcttacgga tggaacatga gaatcatgag tcgtacgtag
    14761 cgtatttaac aattaataca attgtggggg agatggaatt tccttcctca gaacttttct
    14821 attaccagca acaacaattt acattcccaa tcgacacaag tatgaatgta gagattgtga
    14881 cgaataaaaa ggctttagct accgttcgta ataagaaaaa agaactgaaa gatttagaca
    14941 atcatgctta ccaatctgac aatgaaacga attccaatgt cttagatgct ttggattccg
    15001 tggatgaatt ggaaaccacc ttggatcagt caaaagaatc tatgtacaaa ctgagttatg
    15061 tggttcgagt gagtgccgaa tcagtggatg aactaaagcg acgctgtgat gaagtgctcg
    15121 atttttatga tgataccaat gtaaaactag tccggccctt tggcgatatg atggggctac
    15181 atgaagaatt cttaccctta agtaaacgat atatgaacga ctatattcag tatgttacgt
    15241 ctgatttttt ggcggggctt ggttttgggg ctactcagat gctaggagaa ttagaaggga
    15301 tttactttgg ctacaatgtg gatacaggtc gtaatgttta cctgaaaccg gcattggctt
    15361 cacaaggagt caagggttca gtaaccaatg cgctggccgc cgctttcctg ggttctcttg
    15421 gtggaggtaa atcctttagt aacaatctct tagtctacta tgctgttttg ttcggtggtc
    15481 aagctgtgat tgtcgatcca aaagggggtg cgaaacgttc ctacttgaaa agagtaggca
    15541 cagcacttca ttaaatcaat atttaatgtg tgttggagaa ctaaacacac gaatagtcga
    15601 atgacggggt aacgccctga aaggctactt taatactctg actggcagaa acttgagtaa
    15661 acaaggctct gtcagaagct cggtgaagtc ggcagacaga caccgtaaca gataatgctg
    15721 acgaaagatt cccagaggtg ggcgttgtgt cctatatgcc ggaagtctat aaaatatcta
    15781 tggttagaat gtagatgaga ggtctactga cgaatccttg aatgtacagg tctatatcgg
    15841 ggctggatag aaatgtccag tgacacaaac agtgtcgcta ctgtgggtga gtaagactcc
    15901 gcatttatga aaatccatat aacgttaaag gcgttatcaa ggtagcaggc ttatagagga
    15961 aacctaagga tatatgtaca gatagtgtgt ttggaacgtt gaaagctgac aacatggaga
    16021 ttttactgtc tgtgaagtgt tcacatcaga aagttattgt gagattagca atgatataag
    16081 gtgtgttaat gtcagtgaga gtggtggcac agtacctatg aaaccgtgat aacaagcggt
    16141 ggagggatag ccactagtca tatgaagaat agaattacta agaaaaatgg tatgggttcg
    16201 agtaagacta agagatgtac ttgctcctgc aaacaaggag ggtacagacc atgctaaaga
    16261 aaaacaagct ccgctataac gagtattatg atatgcaaca tatttatgac gagttatacg
    16321 cacagagcaa gaacggtaat aacttttata agttacttga aattattggt tcggaacaaa
    16381 atatttgttt agcttaccgt aatctgaaat caaacagtgg cagtaaaacg gctggaacgg
    16441 atggaatgac gatagacgat ataaaacaac tcagcaatgc tgagattgtt gctaccgttc
    16501 gggaaagcct tagcaactac cgccccaaat cagtcagacg tgttttcatt ccaaaagctg
    16561 ggagcgataa aatgcgtcca ttggggattc cgtgtatctg ggacaggctg gtacaacaat
    16621 gtatcctgca agtcctagag ccgatatgcg aaccaaagtt ccataatcat tcctatggat
    16681 tccgtgcgaa tcgaagtgcc caccatgcag tcagcagagt aacgacactg ataaatttat
    16741 caaaatatca ttactgtgtt gacgtagaca ttaaaggatt tttcgataat gtcaatcatg
    16801 gaaaactatt aaaacaaatc tggacacttg gtattcggga taaaagattg atttgcatta
    16861 tcagcaaaat gctgaaagcc gaaattgacg gcgagggtgt cccagaaaaa ggaacgccac
    16921 aaggcggtct gttaagtcca ctgctctccc tcatagtcct taatgaatta gactggtggg
    16981 taagcagtca gtgggaaaca ttccagccga agaaccgcag taaaaatggg tggcttcaat
    17041 acgcaaagaa atataccaaa ctgaaaagcg gttttattgt acgttacgct gatgatttta
    17101 aaatcatgtg ttcaacctat ggagaagcac aaagatttta ccacagtacc gtagattttc
    17161 ttaataaaag gctgaaatta gaaatcagtc cagagaaatc aaaggtagta aatctgaaga
    17221 aaaattcatc agattttctg gggttcaaaa tcaaagtcat tccaaaagga agaacgaaac
    17281 acggttatgt tgccaaaaca gatatgaacc aaaaagcatt aaagaaagcg aaaacaaatc
    17341 taaagttaaa ggtaaaagat atagccagac atacgactgg cttcaatatc agcagatata
    17401 atctgacagt tatcggtatg cagaattact actgcatagc aactaacgta tataacaacc
    17461 tgacagaagt aagttacgct ctcttaccga ccatacgaat ccgtttaagg aacattgcaa
    17521 aatcagttcc atttgaaagt acaagttcag aatttcagag caggacaaaa ggaatcagac
    17581 caaaaacaaa aatagtgatg attgcggata atccgctatt gccgattcaa ggagtacaac
    17641 acaaaaatcc tatgaatttc tcgcaagata tttgtaattt tacaaagcaa gggagaaaca
    17701 aggtgcatga ggacgttgta gttgtaacaa aagaagaaat ccgagcgttg ttggaaaatg
    17761 aaaaccctgc cgatagcgta gagtttaacg acaatcgtat ttcggcatat atcgcacaac
    17821 aaggcaactg ctatgtaatg aatcgtcgag gaactccgtc cactctgata tgtattcaca
    17881 aatcagacaa aggagataac cttgacagat attctaatct ggcatttgtg gaaattccca
    17941 ttgcaaaggc aatcctaaca gaatcagcag acgaagcaaa atcactgtta aaaggttatg
    18001 ttcttaatag tcagcagaag aaaaaactga acagaatacg gacgaattac ggataccaga
    18061 caataactgg gaaataactt ataaatctat ttggatatga tggaacgccg tatgaagcga
    18121 aagtttcatg tacggtgtga agcgggggaa aacttggaga taacatcaaa aagttaccta
    18181 tcgctatagc gaggagggtg gaaagaaacc ttgccagaaa ttgcggatga aatcagtatc
    18241 ctcaacttga cgagtaagtc agaaaatcaa ggtctgcttg atccttatgt gattatgaag
    18301 aaaaagaaag actcggaaag tttagcaatt gatattctga cttttttaac aggaatttca
    18361 agtcgagacg gggagaagtt tcctgtgtta cgtaaagcca ttcgaacggt gactcaaagt
    18421 gatcaacggg ggttattaca agttattaac gaattgcgaa aagacgggtc accaatcgcg
    18481 gaaaatatcg ccgatcatat cgagtccttt accgattatg attttgctca tttgcttttc
    18541 tcagacggct cgattaccca atcgattagt ttagataaac agctaaatat cattcaagtg
    18601 gcggatttgg ttttaccgga tgcagaaacg acttttgaag aatataccac catggaattg
    18661 cttagtgttt cgatgctaat cgtcatttcc acttttgcct tggactttat ccatagtaac
    18721 cgagaaatct tcaaaattgt cgatttggat gaggcttgga gttttcttca agtggctcaa
    18781 ggaaaaacgt tatcgaataa gcttgttcgt gctggtcggg ccatgaatgc tggggtctat
    18841 ttcgtcactc aaaatagcga cgatttatta gatgaacgtt taaaaaataa tatcgggttg
    18901 aagtttgctt ttcgcagtac cgatattcat gaaatcaaga aaaccttgga attctttggt
    18961 ttggatcagg aggatcaaag caatcaaaaa cgcctgcgag atttggaaaa cg
//
LOCUS       DACEDO010000058        18841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEDO010000058 DACEDO010000000
VERSION     DACEDO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.058"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2177)
                     /locus_tag="IWO52_002072"
     CDS             complement(<1..2177)
                     /locus_tag="IWO52_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356328.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="IWO52_002073"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="IWO52_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            complement(4085..4846)
                     /locus_tag="IWO52_002074"
     CDS             complement(4085..4846)
                     /locus_tag="IWO52_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0356329.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            4946..6667
                     /locus_tag="IWO52_002075"
     CDS             4946..6667
                     /locus_tag="IWO52_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356330.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            6682..8466
                     /locus_tag="IWO52_002076"
     CDS             6682..8466
                     /locus_tag="IWO52_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356331.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8847..10400)
                     /locus_tag="IWO52_002077"
     CDS             complement(8847..10400)
                     /locus_tag="IWO52_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0356332.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10748..13162)
                     /locus_tag="IWO52_002078"
     CDS             complement(10748..13162)
                     /locus_tag="IWO52_002078"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0356333.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            complement(13589..15607)
                     /locus_tag="IWO52_002079"
     CDS             complement(13589..15607)
                     /locus_tag="IWO52_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0356334.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15977..16633
                     /locus_tag="IWO52_002080"
     CDS             15977..16633
                     /locus_tag="IWO52_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0356335.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            16633..17589
                     /locus_tag="IWO52_002081"
     CDS             16633..17589
                     /locus_tag="IWO52_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0356336.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            17589..18155
                     /locus_tag="IWO52_002082"
     CDS             17589..18155
                     /locus_tag="IWO52_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0356337.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            18591..18821
                     /locus_tag="IWO52_002083"
     CDS             18591..18821
                     /locus_tag="IWO52_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356338.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         5541 a         3771 c         3343 g         6186 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaaact aactatccat caataacgtg ttttattttt taaaatttct
     3481 ataaaaaaaa caatcattct taaaaagaat atataaaaat aacaataacc tttgtgattt
     3541 ttcttgtttg aagaatgtta gaagaaaaat ctctaaatac ttataattat tttataagca
     3601 aaaaaatgct atcggtagta tcaccgatag ctgaggaaag tcacagtagg gactatttga
     3661 ttaaaaaata tgtttattca atccatctat ttatttttgc cttattaaca tattgtaata
     3721 atgtaagcaa caatgtattt taagtgtaat gcttttatgc acctatttaa tttatcattc
     3781 tcatctgata caatctcact cataacaata tgaatttttc ttctcattag tctcagttca
     3841 ccatgaatta tacataacac ccaatacaat tatttttcca tactttttta agaggacaat
     3901 tacatcgtat atatcgctaa gaattgcaac cattccattt ttaaagacat atttcctcta
     3961 aaaaactaaa attctatcta tccacgacta ataatccccc acattaaacc cgtaatcgaa
     4021 ccagaaaatt tttctttata actaaaacaa ccatcatttt cgagttatct taaacacata
     4081 tttcctattt tttcatttct atccatgcat gataatattc agttctgata ttgttttcat
     4141 ccgtattcgt caatagatac tggccgatca cttctcctgt tttccatcct ttttcataca
     4201 ttctttctct gatttctaaa atattattac gttccgcatg gttcatctcg gatttcaata
     4261 agaatcgacg ataaacagcc gtatcttttt tctcccaaat cacgtcttga gtaaataaat
     4321 gtacttcact agggtcgata aaaaatccat acatcgtctg tgaactggaa gctggcatac
     4381 tcatcatccc aaagcttgaa tagttaggta gaaatttttt gatttccaca atatcttcaa
     4441 actccgttga gatcatacag tcaaaaggga tctcttctac aggaaattct tcctcgtttg
     4501 tatcgatcat caatttcaac tgtttttttt gctcccttat ctctttttgt ttttgtttca
     4561 tcaccaccag ttctttatgg attcttcttt ctgtttcatc aagtgtctca tataactccg
     4621 tcaatgtttt tccatataaa ttcttcatct gtttgatcgg tatatctaat tttctataaa
     4681 aaacgatatc ataaagcata aaaatatcat cgatatcaaa caatcgataa tcattctctt
     4741 cttgtctact tgaacggatt aatccttgtt catcccaata gcggatcgtt gattttggca
     4801 aatcaaatag ttccgatact tggccaatcg tatacatttt tttcattttt tctcacctct
     4861 ttttcagttt attctttttt ctattgacct tcaagttact tgaaggtata gaatcgaaaa
     4921 gtatcaggaa aaaaggagga ataatatgtc tatcttaaaa acattcttac agaaaaacaa
     4981 aggactcgct ctccttacct ttgtgatgat ctgtttagaa atcgccggta cattaggtgt
     5041 accaaaatta gtagcaaagc tgatcgacac aggtatagca agtggcgaag cctcagttat
     5101 aaaaaaaatt ggagttgaaa tgctgcttgt agcgtttcta ggaacaatcg ctgcagttgc
     5161 ctctagttac ttttcagcat tggcttccgc taggtttgga ttccaaacgc gggagaaata
     5221 tttccgtaaa ttccaacagc tgtcaatgaa ggatatcagt cgttttagtt ctggatctct
     5281 tttgacaaga atgacgaatg atgtcgataa tgtccagcaa atgattgttt tattttgcca
     5341 aatgatctta ccagcaccgg ttatttgcgt tttcaccatt ctcatgatgt ttcgttattc
     5401 tctattacta acatgggtta cattgatctc tgttgttttt tatgtatgga ttgtttatcg
     5461 actgatgaaa cgaggggcac ctttatcttt gagtatccag ccaaaaatgg atcggatcac
     5521 catcacattg agagaattct ttactgggat taatatgatc cgtgcctttg ataatcaatc
     5581 atatgaagaa aagcgaacga ataagagctt tgctgactat gctaatcaaa tgatcaaagt
     5641 gaaccgaata tttgcttggg tcactccaat tgcttttttg ctcatgggaa tcgtttatgc
     5701 ttctattttg tggtttggcg gaaatcttgt agcagaagga gcattacaaa tcggtgtcgt
     5761 gactgctgta gtcgaatact ctatgctgac acttgcttac ttgatgatcg cagctatggt
     5821 attggtcatt atccctaaat cgtttgcctc attacgacgg atagaagaaa tactgcaagc
     5881 agagatcgaa attgaggaag agacgatcaa aacagtagct gaaccaacct ttgatcagac
     5941 aaatgcagct gcttttgaac atgtcacatt taaatatact gaaatctctg atccagttct
     6001 agaagatatt gatttcacaa tacctaaagg aaaaacaaca gcgatcgttg gcgggaccgg
     6061 atctggaaaa agtagtttgg cgaaacttct cttgagactt agtgacccta cgataggaaa
     6121 agtaaccctt ggtggtattc ctcttactca aatgactcaa gaacagatcc gctcccacat
     6181 cagttatgtc cctcagaagg ctttcttatt cagcgggaca attttaagca acttgcagat
     6241 gggaaatgaa tatgcctcaa cagaagagtt gtctaaagcg atcaagatcg ctcaattaga
     6301 agaactagtg gacacccttc ctgacggatt ggacagcttt gttgctcaag gcggttctaa
     6361 ttattcaggg ggccagaaac agcggatatg tattgctcga gcgttgatca aaccagcaga
     6421 gatctatgta ttcgatgata gtttctccgc tttagactat aaaacagatg cagcattaag
     6481 agcagccctt cataaacaca tgtcggagaa aactctcgtg attgttgctc aacgcctcag
     6541 tacaatccaa caagcagata acatcattgt tttagatgaa ggaaaaatcg ttggacaagg
     6601 tactcatgaa gaactactgt ctaattgcca aacttatcag gagtttgcaa aatcacaagg
     6661 aatatagaaa gaagggatac gatggaaaag aaaaaaacgt tgaacacgcg tgctttgata
     6721 cgtttttgga aaatgatcaa acctgaacat aaatattttt acggattgtt tctatgcagt
     6781 ttattcggga acctattagt cgtagccatg cccttgatca tgggaatcgg gatcgatgat
     6841 ctcttatcag aaataagaaa aacaggtatt ggacagcttt ctttcagtga tatcaaacaa
     6901 gcattgttgt tccctgtctt attgctgatc ggctgttcag tcttaagcag catcacttca
     6961 tttattcagg aacggacaat ggcagcgtta agcgaaaaga tcacattgag ggtgcgaaaa
     7021 gaaatcacaa aaaaattcaa aacattaccg atgtcgtttt ttgacaatca tcaagtaggt
     7081 gacatcatta gtcggacaac taccggtctc aatcaacttt ctcaagtgtt attgacaggt
     7141 atcaatcaat tttttacttc tgtcgtaaca atagctgctg ctggtctgat gctgttttat
     7201 atcgaaagca agcttacttt gctcgtctta ttgctgatcg ttggcagttc aattgttaca
     7261 actaagattg cgaacaaaaa caaacagatt tctgaacaga atcagtatga actgggtgtt
     7321 ttaaataatc gaatggaaga aatcttagct ggtaatctag tggtcaaaac attcaatcag
     7381 caagctcatg cagaagctgc tatacacgaa gtaaataaaa agcaatacaa cgcttttaaa
     7441 cgtgctcaat ttatgaactg tgctatctat ccggccattc gtttcattaa tcaactcgcc
     7501 ttcatcataa gtgcggtatt cggtgctatg cttgttctat caggtggtgt gacgatcggc
     7561 ttcttacaag cttatcttca gtacatcaat cagatttctg aaccaatttc taccgcttct
     7621 tatgtgatca actcgatcca atccgcaatg gcagcaatcg ataggatatt tgagatatta
     7681 gatgaaacag atgaaattcc agaccttcca gatgcaagac ttttagaaca accgaaagga
     7741 aaaatagctt tcaaaaatgt acagtttggt tacacatcag ataaaatatt gatgaatcaa
     7801 gtcgattttt cagtaaagcc aaaacaaacg gtagcgatcg ttggtccaac tggtgcagga
     7861 aaaacgactt tagtcaatct gctgatgcgt ttttatgaac tgaataaagg aaagatcgaa
     7921 ttcgacggtc ttgatattac caagctttca cgtggagaac tacgtcgttc ttttgggatg
     7981 gttttacaaa atacctggct gtttgaagga acggtagctg ataatatcgc ctacgggaaa
     8041 atggatgcca caagagaaca aattatcgaa gcggctgaaa tcgcacaatg tgatcacttt
     8101 attcgaacac tgccagacgg atatgatacg atcatttcta gcgaaaacgg cacgttatct
     8161 caaggacagc aacaattatt gacaattgct cgagtcattc tagcgaatcc tcctgtcgtt
     8221 attttagatg aagcaacttc tagcgtagat acacgaacag aagcattgat tcagcaagca
     8281 atggaagctg tgacaaaaga taggacaagt tttgtgattg cacaccgttt gtcaacgatt
     8341 gaaaatgcag acatgatcct tgtaatgaag aatggcgata tcgtcgaaaa aggaacacat
     8401 tctgaactac tccaatcacc aacgctttat gccagtcttt ataatagtca atttcagata
     8461 gcgtaacaaa cattatctaa aaagctatcg atcatgtatg attagtccat gatcgatagc
     8521 ttttttaaat gtactagtgg tgccactaga aacacaaaga ataatactaa tgatactttt
     8581 tggatagctc cttcagtttc gttcggtaag tagtgacaaa agacttgtca caatcccttt
     8641 ttacgaataa acggtgttca agagacaacc tatcggcaat aagacacgat cttgtaaaat
     8701 acgagaagct atacttcaaa atcgcttctt gttgctcttg tcaactccga atcacgagtt
     8761 acactattca acaaatatga gaagctgttt tttgaatttg tttcttgtga ctcggtgctg
     8821 aacgtcaggc tcacaatctc tcttctttaa tctccttgtt ctacttgcga tagttgctga
     8881 acgaattgcc atgtttcccc gttatcagtt gaacggaaga ggcaggcaaa cattttccct
     8941 gcatcgattg gtcctttttt caacaaataa aattttgtct ctaacgtatt cgcgcttatt
     9001 tgagtaactt catttggtga ttgaaaaaga tctatccccc ctgtctgata gttttccgga
     9061 atcgacagaa ctgattcttt gaatgagcgt cctccgttgt ttgtataaaa caaactttca
     9121 cgtgtcgaaa caaaacctaa agaagttgta acgaagctga cattttgtac tggctgattg
     9181 atcattgtac ttccactttt tatccaagtg attccgccat ccatagtagt gtaaatgtac
     9241 aaagtttctg cagacatccc actggctgat gtacagataa atagaccatc gttgccataa
     9301 aataccgctt ttcgataacg tatcggctga actgattggg tgataataga cgtttgccat
     9361 gttttaccat tatctcttga agtcagcaga cgtacatcat ccccagaaga atagataaac
     9421 catgagaaat cggcggaaat atcatatgtg ttgtccatcc aataaccttc aggaatcagc
     9481 cccgttgtca gggtgtattc gccaaaacgc aaccattgcg aatcaatcgg cacaaatacc
     9541 caattagcac cactatctgt tgtcatatag agagagtgcg tctctgtatc aagtttgaga
     9601 tcatttatct tttgtgttgt caaatttacc ttttgcttag gatcagctaa caacccttca
     9661 tttgtctttt gtatatgcgt atcgttagtt gagttcattg tcatgtgaga agcagcattt
     9721 atatgagaag acatttttgg ctgagtgtat ctcccataat agattgttcc agctaaaaga
     9781 atgaggaaaa aaccagtgac taatgtactc atcttagttg tcaggtatcc accaggcgaa
     9841 gactggcttt tttcccatct gggttcgttt tctagtaaaa aaatatggaa tgtgttcatt
     9901 ccgaattttg atttgctttc gcctaagtag ataggtaaat catcaagtga tctcagcaat
     9961 aactggcaat cacttttttg tatccgaaaa gcttgactcg tcgaagtctt ctcaaaataa
    10021 agatagtcat catcttgaga agttggttta ttatgtaaaa actgttgtgc tctttgacta
    10081 agccattgat cgaaatgacg cgacaattga tggtaacgga gccaaataag tagaagaaaa
    10141 acaataagca caacaattgt tattccgcca actccccacg aaaagtgata caaattattt
    10201 aatttttgca tcccttcagc tgaacggact actgcactca tatggcgata taggaacgat
    10261 acagctccga aaaaaagcaa accagagaaa aatactaagc ttaaaagctt gaacattatt
    10321 cttttcgctc tttttttcgc tttctcgata attttttgtt tagctgataa tacttcgata
    10381 ataaatagtt cttgatccat ttgtctctcc ttttctcata cttcattcat tatgcgtcaa
    10441 aatccataca atcactagtc ccactaataa aaaatttatg aatttggtat gtactctcaa
    10501 tttcaggtag tttatacatc aaaaaatact aagattaata gacaaaaatt tgatgattac
    10561 agagatctct tacaagacca ttcagaagaa aaagacattg aacgtgtttg agaacatgaa
    10621 aatcaaaacc tttttgatca atcaattaat caatttgaaa taacaaaaaa tctgagacag
    10681 aatgaagttc attctggccc agatttgatt ttgcacaact catcagtcct tattgacaaa
    10741 gagccaatta attagcaacg atattgacta acttgtttgg taccgcaata acttttctga
    10801 ctgttttgcc ttcaatatat tcttgcacta tttcattggc taatgctgct ttttctagct
    10861 catcttttcc taagtctcgg ggaacattgc ttttcgcacg aactttaccg tttacttgga
    10921 aaacaacttc tacttcgtct tcaacaagtg ctgcttcatc gtatgttggc catggtgcgt
    10981 aagaaatacc accatcattt ccaaggattg cccatagttc ttcacccatg tgaggagcaa
    11041 tcggtgcgag tagttgaaca aatccttcga tatattcata aggcagagcg tccactttat
    11101 aggcttcatt gataaagacc atcaattggg agattgctgt attgaaatga agattttcat
    11161 agtcttctgt cacttttttc actgtttgat gatacacttt atcaagcttg ccatcattca
    11221 atgtagtgat acggtcacgc attttattgt tctcatcaac gatcaaacgc catacacgat
    11281 ccaagaattt acggcttcct tctaagccat tttcgctcca agcaatcgaa gcatctaatg
    11341 gccccatgaa catttcgtac atgcgcagtg tatctgcacc gtaagtttcg accacatcat
    11401 caggattgac tacattacca cgagattttg acattttttc gttgttttcg cctaagatca
    11461 ttccttgatt gtatagtttt tggaatggtt cttttgtagg aacaacaccg atatcgtata
    11521 agaatttatg ccagaatcgt gcatatagta aatgaagtac cgcatgttct gctccaccaa
    11581 tgtagatgtc aactggtagc caacgcttca acttttcata atctgctagt tcttttttgt
    11641 tatgagggtc gatataacgc aggtaatacc atgagcttcc tgcccattgt ggcattgtat
    11701 tcgtttcgcg acggcctttt ttacctgtta caggatcaac gacattgacc cactcctcga
    11761 tatttgctaa tggagattct ccagtaccgc ttggcttgat gttttttgtc ttaggcaacc
    11821 gtaatggtag ctcttcttct gggacagctg tgactgttcc gtcctcccaa tggatgattg
    11881 ggattggttc accccagtaa cgttgacggg agaatagcca gtcacgtaag cgatagctga
    11941 cttctttttt ccctacgcca ttttcttcta gccattcatt cattttatcg attgcttctt
    12001 gtttgttcat gccgtctaga aattcagagt tgatgtgcgt accatcttct gtgtaagcag
    12061 ctttttcaac atctccacct tcgataactg gaagaatttc tagtccgaaa gtttgagcga
    12121 attcaaagtc acgttcatca tgtgctggaa cagccatgat ggctcctgtg ccgtatgaag
    12181 ataatacata atcggcgatc cagattggga tttccttgcc attaactgga ttgattgcat
    12241 aagcaccagt aaatactcct gttttttctt ttgcaagatc cgttctcttt agatctgatt
    12301 ttttagctgt ctcttcgata taagcttcta cagcagcttt ttgttcaggg gtcgtaattt
    12361 ctctaacaag atctagttca ggtgcaagaa ccgagtaagt tgcaccgaat agagtatctg
    12421 gtcgtgtcgt aaatacggta taggatttat ccgtaccagc cactttaaat tcaatatttg
    12481 ctcctactga acgaccgatc caatttcttt gcatttcttt aatgctttct ggccaatcaa
    12541 ctaattctag atcatccaat aagcgatcag cataagcagt gattttcagc atccattggc
    12601 gcattggttt tctgactaca tcatagccac cacgttcact tttcccatcg atcacttctt
    12661 catttgaaat aactgttcct aattcaggta cccagtttac cgctacttct gcttcataag
    12721 ctagaccttt ttcataaagc ttcgtaaaga tccattgtgt ccatttataa tactccggat
    12781 cagttgtatt cacttcgcga ttccagtcat aactaaatcc taaagaattg atttggcgtc
    12841 ggaatgtttc gatatttttt tgggtaaact cagctggatc gtttcctgta tctagcgcat
    12901 attgttcagc aggcagaccg aatgcatccc accccattgg atgcagcacg ttgtatcctt
    12961 gacttctttt cacacgtgaa aggatatccg ttgccgtata accttcagga tgccctacat
    13021 gtagcccttg accggatgga tagggaaaca tatccagtgc gtaaaatttt ggcttttcag
    13081 gatcatcatg ggtgttgaaa gtattcgttt ttgcccagta tttttgccat tttttctcaa
    13141 tttctttatg attgtaactc atttgctgtc ctcctgttct attgaaaaca cttgtttcta
    13201 ttataaatat aacgaaaaaa ccggcagaat ctgtttattt ttatttcctt ttgctttgtt
    13261 ttctcaaact tcacaaaaga aataaaaaaa tcctataaaa agattgctct ttttatagga
    13321 cgtaatctac gcggtaccac ctaattttac cttagtgaaa aggcctcgaa cgttgtaacg
    13381 gccgtcaccg ccgcttgctt actcgggtat tcagcaaggg caactcaaag gcgagttcaa
    13441 aagcatccgt gcacattctc accagccatg cgctctctga aacttcagtc tttttactac
    13501 ttccttttca agtgttgtca tatttaagta gccttatagt aacaaatatt tttgtggaat
    13561 tcaatcgtta tttcttcgaa cagctatttt atttagctac aatcaacgtt tgacctggat
    13621 aaataaagtt gttcttaata ttgttccatt cgattagttg agccattgta attccatgac
    13681 tgtcagcaac actccaaaca gattctccag atttcacagt ataggttttc gaaccagtaa
    13741 ctgattgttg gtttgttgat tgagatgaac ctttcttcac aatcacttgc tgtcctggat
    13801 agatgaaatt atttttgata ttattccatt cgattaactg atccatcgtg atatgatgtt
    13861 tgttcgctac gctccaaact gactcgcccg ctttcacggt atacatcgta ttaccagtgg
    13921 atgatgtact tgtcgtagca gacgtattcg gtgtatttgg tttcgctgca gaagtagttg
    13981 aacctgtatt tgtatttgcc gaagttccac ctttaacgat caattgctgt cctggataaa
    14041 tgaagttgtt tttgatatta ttccattcga ttaactgatc catcgtgatg ccgtttttat
    14101 ttgccacgct ccaaacagat tcacctgctt tcacggtata agtgccttga gacgaactgc
    14161 tgccagtatt aactgaactg ctgttatttg tattggttga cgaacctgta ttacctgacg
    14221 tgctgtttgt tgatccactg tttgaaacag tcagttgttg tcctgggtag ataaagttat
    14281 tttggatatt gttccattgg attagctgag ccatcgtaat tccgtattta tttgagacgc
    14341 tccaaacaga gtctccggct ttaactgtat atttcgttcc tgaagctgta ttagaagaac
    14401 ttgagctgga cgaactagag ccacttgtat tcccagtatt tgagccagta tttgctccag
    14461 cattagaaga accgccattt gatacgatta gctgttgtcc tggataaacg aaattgttct
    14521 tgatattgtt ccattcaatc aattgagcca tcgtaattcc gtgatcattt gagatcttcc
    14581 aaacagaatc accagattga acagtgtatt tcgcaccagt tgtttgtcct ccgtttgatg
    14641 taccagcatt tcctgaagag ttggtattgt taccggaatt attcgtcgaa gagccatttt
    14701 cctggctacc tttgattgtc agtttttgtc ctggataaat gaaattgttt tggatgttgt
    14761 tccattggat caattgatcc attgtgatac cgaatttatt tgagattccc caaacagaat
    14821 caccagattg gacagtataa gtggtcgaag ttgctgaacc tgagtttgta ttaccagaat
    14881 tgcttgaacc gccagtacta cctgcacttc cagtattatt gccacctgta gtcgctccac
    14941 cgcctgcgtt tccgctagct ggtgtatcat attgggtcaa tccgtaagtt gcgatgatat
    15001 tatttaattt gcctgcgtaa ccaggatcag tcgcataacg ccctgtcaaa caagctgtcg
    15061 catctgtata ggatttcgtg ttgctcttcc aagtaccggc atagtaataa ccgttgccaa
    15121 atgaagtatt tctaagtaca taggcattgt cattgaaaga ttcagcaaaa gaaggatatt
    15181 gtcggaaagg ctccttttta tttacccatt gattatttaa aaattcaagc gtatccatgt
    15241 agacggtttg tccttgatag ctgcctttta tcccaaaaag attataataa ggtgcttgag
    15301 ataacgcact actgccccaa ccgctttcta caactgcttg tgcgatcatc actgatgcat
    15361 atagatcgtt ggcttgagcg attggttgag cataagtagc aatttctgtg ataaatgccg
    15421 ctgtattgat tcttgcgccg aactgaacag gtgtttcatc cgcttgaaca ggaaccggct
    15481 gtactaatgg ggcagcgaca gaacagccta ccatcgcagt tcccacgatt gctgcccctc
    15541 gttttgcttt tcgaaaacgt ttggtctgtt ctaaacgtct ccgttctttt cttgcaatgt
    15601 tttccatctt ctatcctcca acttcaattt atctattatt aataattata acttctctta
    15661 atatttatta aaagttattt aagagctttg ttgttttttg taatcatatt gtaatttacc
    15721 gtaatgaaca acttctgttc attttcaagt tcttcctacg tctacgttct tgtttaaaag
    15781 gatttcgaaa aacactgatt ttatttccct aacatttcat ttgcattacc aaaaaatgtc
    15841 tttccgatta cagttatttt ttttataaga atattggtta ttcaaagagg aatcatacat
    15901 tggttttatc tctttaccgt ttttttcgct ggaccaaaaa tgctataata agtcattgct
    15961 tcaaaggagg aattgtatga aatataaaac atcatttcag gtggctggta gcttctttct
    16021 tgtcctgttt ttcattttgg gctggatcgt aaagttccat ctgtcgtggc tttctggttt
    16081 cgatcagtgg tttacaggca ttgtcagagc ttcttatcct gatctgaaca gctaccaact
    16141 ctttatgact aaattcggta atcctcttac cgtcatcatt ttatttgctt gtgcggcttt
    16201 atggatgtgg tataagaaaa aaagggcaga aacattttgg ttcgtttctg gatttattgt
    16261 aattgctgga atcatcaacc cggtgatcaa actatttttc atgcgagagc gtccttcatt
    16321 ggcgcactta gtagttgaaa caagttacag tttccctagt ggacatgcag caggcagtat
    16381 gattctttac ggttctttac tatttctgct tccagctttt gttcaaaaaa gaaccttacg
    16441 cacattgctt caattgctga tgatgtttat tatagtaagt gtcgggataa gccgcattta
    16501 tctgggcgtt cattttccta ctgatatcat cggcggctac ttactcagtc tttcttggct
    16561 gtgcctgact tatccagtgt atagagaaaa aagaaaacaa atagataact cttacagaaa
    16621 gaaggacttt taatgacttt tccttattca gaaggaaata aaagatatca ttcttggaat
    16681 tacgctttac gaaatgagtt tggcgggaaa attttcaagg tgccgatcga tggaggattt
    16741 gactgtccga atcgagatgg gactgttgcc catggcggtt gtactttttg cagtgtatct
    16801 ggttcgggag acatgatcgt tgcacctgaa gaccctcttc caatccaatt ccagaaagaa
    16861 atcgacatga tgcatcaaaa atggcctcat gtccaacaat acatcgttta tttccaaaat
    16921 tttacaaaca cccatgcgcc agttgaaata atcaaagaac gatttgagca agtagtcaat
    16981 ttaccaggtg tcgtaggatt atcagtcggt acacgaccgg attgtctgcc agatgaagta
    17041 gttgattacc ttgctgaact gaacgagcgg atgtatttat gggtagaact tggattgcaa
    17101 acaacttttg aagaaacttc aaaactgatc aaccgagcac atgattatca aacttattta
    17161 gatggcgtgg ctaaattacg caaacataat atccgtgttt gtacgcattt gatcaacgga
    17221 ttaccaggcg aaagtttaga aatgatgaaa gaaaatgtca gacgcaccat tttagattca
    17281 gatatccaag gaatcaagct ccatttgatg catctgatgc gcaaaacgag aatgttgcgg
    17341 gactatcatg aaggtcgcct tcaattaatg agccgtcctg actatgtcaa tgtcatttgc
    17401 gaccagctgg aaatgatacc aaaagagatc atcatccatc gtttgacagg ggatgcgccg
    17461 tgggattcct tgattggacc aatgtggagc ttgaaaaaat gggaagtctt gaatgcaatt
    17521 gatgaagaac ttcttcgcag agatagcttc caaggaaaat atgatgtacg gaaaaaggtg
    17581 agcgtctgat gttgcaaact gctttgcatt tcagccatac attactgcaa gaaatcttgc
    17641 aaccagggga tcacgtcgta gatgcaacga tgggcaatgg ccacgatacg gtttttctag
    17701 cagaacacat cggaaaaact ggtcatgtct atagttttga tatccagcaa caagcaatcg
    17761 atgctactcg tgaaaggcta gaacagcgtc agttagaaga acgtgtctca ttgtttctac
    17821 aaggccacga aacgttagga gaagtgatag ctgaacaaca aaatctcaaa gcagggattt
    17881 tcaatctagg gtatctccca aaaagtgata aagcaatcat cactatgcca gagactacac
    17941 gcacagctat ggaagaaata ttgaaacgac ttgtgcctcg cggacgattg atccttgtcg
    18001 tctactatgg tcatgaaggt ggagaaaaag aactggatat ggttgattct ttttgttcca
    18061 aactgccaca ggaaacatat aatgtgctga attatcggtt tatcaaccaa aagaaccagc
    18121 cgcctatttt atattgtatt gagaaaaaaa gataaataaa aaacgcaaaa cataaatgat
    18181 atgttttgcg tttttttctc taatactcag gttagagcgt tgatttcata acctctttat
    18241 tcacggtgct caaaagctta tatctgcggt aataagtcca accaaatcaa aaacatgaga
    18301 agctgttttt gattggctgt tcttattact tgatgcagga cagctttttc acaacctctt
    18361 tattcaaata caaattgaaa ttgaacacat ttttttgaca tataacagat atataaaaga
    18421 atggcaaaaa cattgatata taagcatttt taaaagattt tatatttttg tatgtcattc
    18481 atatatcaat agctaactta atatcagtca aattatgccc cttttttgcc cctcaaatat
    18541 cgctttacaa caagaacgta agttcgtata atcgttttga ggtgattttt atgatggaag
    18601 aatttattag aaaaaatatc agtgatgaat atgcagactt ttatgatcaa agcagtaaaa
    18661 aagataaatt ccagatggat gtttcaatct tagctatatt agctttttcc gaaaataaac
    18721 aacctgtaac tgcaaaaaaa gaaacagtat tctctgaagg caaaataaaa actcgatata
    18781 tattagaggt agaaactaat tttaaaaaca gatcggagta atggttatgc tttttaatga
    18841 g
//
LOCUS       DACEDO010000059        18840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEDO010000059 DACEDO010000000
VERSION     DACEDO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.059"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1364)
                     /locus_tag="IWO52_002084"
     CDS             complement(45..1364)
                     /locus_tag="IWO52_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0356339.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IWO52_002085"
     CDS             complement(1361..2014)
                     /locus_tag="IWO52_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0356340.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="IWO52_002086"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="IWO52_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="IWO52_002087"
     CDS             complement(2724..3440)
                     /locus_tag="IWO52_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0356341.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3490..3699)
                     /locus_tag="IWO52_002088"
     CDS             complement(3490..3699)
                     /locus_tag="IWO52_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356342.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3976..5249
                     /locus_tag="IWO52_002089"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="IWO52_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="IWO52_002090"
     CDS             complement(5356..6387)
                     /locus_tag="IWO52_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356343.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="IWO52_002091"
     CDS             complement(6394..7224)
                     /locus_tag="IWO52_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0356344.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8027)
                     /locus_tag="IWO52_002092"
     CDS             complement(7221..8027)
                     /locus_tag="IWO52_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0356345.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="IWO52_002093"
     CDS             complement(8033..8857)
                     /locus_tag="IWO52_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0356346.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="IWO52_002094"
     CDS             complement(8847..9947)
                     /locus_tag="IWO52_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0356347.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="IWO52_002095"
     CDS             10125..10910
                     /locus_tag="IWO52_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0356348.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="IWO52_002096"
     CDS             10943..11326
                     /locus_tag="IWO52_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0356349.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="IWO52_002097"
     CDS             11402..11533
                     /locus_tag="IWO52_002097"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0356350.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="IWO52_002098"
     CDS             11502..11738
                     /locus_tag="IWO52_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0356351.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="IWO52_002099"
     CDS             complement(11816..12787)
                     /locus_tag="IWO52_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0356352.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13439..15061
                     /locus_tag="IWO52_002100"
     CDS             13439..15061
                     /locus_tag="IWO52_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAQ0356353.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            complement(15347..16723)
                     /locus_tag="IWO52_002101"
     CDS             complement(15347..16723)
                     /locus_tag="IWO52_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0356354.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(16723..17379)
                     /locus_tag="IWO52_002102"
     CDS             complement(16723..17379)
                     /locus_tag="IWO52_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356355.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(17389..18666)
                     /locus_tag="IWO52_002103"
     CDS             complement(17389..18666)
                     /locus_tag="IWO52_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0356356.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(18685..>18840)
                     /locus_tag="IWO52_002104"
     CDS             complement(18685..>18840)
                     /locus_tag="IWO52_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0356357.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         5801 a         3888 c         3044 g         6107 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatgatct
    13501 agttcctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg aatttattta
    13561 tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc cagtgattct
    13621 gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac aaaccattaa
    13681 ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tactccttcg aggagaaaat
    13741 cccccctttc tcaacttttg gaaagaatta tgttcgccga tttagagaaa cgacggtgtt
    13801 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
    13861 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
    13921 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
    13981 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
    14041 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
    14101 agaaaaacaa tttgcttatt ctgtccatac ttcatgcgac gacaatggat ttattcttag
    14161 cacaatcatc actcctagaa atattcatga cagtcaagtc gcctttcagc tcgtcaaaca
    14221 atcgaagaga ctttttcctg aaatcaattg tgttgtcgct gatgctgggt ataagacacc
    14281 taagtttgtt cactttttga ctcatttgaa ccttcgacct tgtttgcctt attcaaggcc
    14341 caaagggaaa aaggagctat tatcaaaaaa tgagtttctc tatgacgagt attttgattg
    14401 ttatatttgt ccgcaagatc aaatgctcgc tttttcaacg gttactcgag aagggtaccg
    14461 agaatacaaa tccaatccaa aagaatgtgt gaactgtcct ctgctaaacc aatgtaccat
    14521 aagtaaaaat catcaacgag tcatcacgag acatgtttgg ggagatctga tggatgaagt
    14581 ggaacatctt cgcctaacag atctgaataa atcaatttac aaaaaacgga aacaaacgat
    14641 tgaacgaata tttgcggatg caaaagaaaa gcatgggatg cgttggacca agtatcgagg
    14701 cttggaaaaa gtcgcgacgc atacgatgct agtgtttgct gcaatgaatt taaagaaatt
    14761 agccacctgg ctatggaaag gcaaggagcc tttatttttt tgttcaaaaa ttcgaaacga
    14821 agtagataaa aagctgttcc aagctcgagt tacgagtttg gaacagcttt tgtctacagt
    14881 ctgcgatgtc ggagacatct cgttttctat taggaatcaa ctcaggttca aaagaagcct
    14941 cccggtctct gggaacatca atgtttactt taccaaagct cgttttaagc gtcttattgc
    15001 cataaccgtt tcgtcgattg tcatgtttct ttggctcttt agaatgggcg tcatacccta
    15061 aatggttatt caattctcct tgaagcattt tttcaaataa gggaccaaac acatctttta
    15121 aaacatcttg catatcctct acagattcgg gttgataagt attcaaaatg gattcagcta
    15181 acttctcagc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
    15241 ttttagaaaa ataaaaaccg tgaagcaacc acccgcctag gattgatggt tacttacacg
    15301 gtttatatta cactctcatt tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa
    15361 ttaataattc ttttggattc cttcttaata tattgaatga agaaataatc aatgatacaa
    15421 gaagtactaa actcataaat ataactacat aaatcatgaa tttaggtaat acattgatat
    15481 ctaaacttga taatgtcttg ttaaatcctt cagcttctgc cccaccacct aattggctag
    15541 atgcagattg tttagctatt tgtttagcta tatcaccagt aaccttattc aatatattat
    15601 tcccaagctt gtcagctgta tattgagcta agaaatagga accaacgaat gcggggattg
    15661 atataaatac catctcaatt aggaattgac caaaaatttc taattttgat atacctaatg
    15721 atagtaatac tgctatttct ttctttctag cattcatcca taagaataat aataatgaaa
    15781 ctacaactcc tgcaaatatt aatgagccaa aaaataattt atttgaaatt gagtaaatac
    15841 ctgatataga ttgttgtaat gcagggtaat ttgatgaact tttaatcaaa ttatattctc
    15901 tccaatttat atctaatttc ccaagatctt ttattacact atcaagattc ttatcacctt
    15961 ttacaaagaa tgttgcatct tggtatacag ctgtatcttc ggtattacca taaactttag
    16021 cagctgtatg aatatcagtg attaatgtat tttcgtatag ttcttgtgct gcacttacta
    16081 caccgctatt atgcccatcg aatagacctt taatttctac ttctactgtt tcatctgcac
    16141 ctttctcatt atcagcatca aataaattag attttatttt tatcttatct ccaactttaa
    16201 ggttattttt ttcagctaaa tctttatgca ttaagatttt atttttatct tcattttcta
    16261 aatgctttcc ttctactaat ttatatgctt ctgatacaaa ttttgtttct tttgttgagt
    16321 cgttaacccc agttaacata actgttcttt taaaattctt tgctctttca ggtgattgat
    16381 tcgcaagagt ctcttgagtt tcaataatat cataatcaac taaatctgca acactgttta
    16441 ttctttttac ataggagtct atactatctg tttgagatat ctttttaata tcttcacctt
    16501 ttacatttcc cccacctcta ggtgttcctg gatttacttg tctatttatc tccatagaaa
    16561 aactatttgt tatattagca aatgtttctt ttgaagctct gtccgtagca tctttaatag
    16621 ataaactaat gagacttaaa gttgacatag ataaaataac taatatgata attaatgatt
    16681 ttaaactttt tctagttaca taagcaaatg cattttttat cattattcaa cctccaaatt
    16741 cattttattt acttttttca actttttacc acttagttct aaaatgatat ccgcagaatc
    16801 tgctacttcc ttactatgtg ttacaactat tacacattta tttctatctt tagctaatgt
    16861 ctttaatata ttaattattt ctccagcagt aacactgtct agattaccag taggctcatc
    16921 agctagtatt attggagcat ctgataccaa tgctctagca atagctactc tttgttgctg
    16981 tccaccagat aatttcataa catttctttt tatttgtttt ttatctaaac ctagttcaaa
    17041 caagatactt tcatctgctg atttatttac taatctaata ttttcaattg gtgataaata
    17101 atctattaaa ttatagtttt gaaatactaa agatatatta ttttttctat gattactata
    17161 tcctttattt tgtatatctt cattcttaaa caatattttt cctgtttgag gtttatcaag
    17221 tccagcaagt aaggaaagaa gtgtagattt tcctgttcct gactttccta ctatcgcata
    17281 aaacttccca agttcaaatt tttgatttac tcctgacaaa actttttctt tagaatttgc
    17341 ataactatat gctacattct ttatttctaa tatatccatt attttctcct aacttatttt
    17401 tgataagatt tctttaggct tcttgattaa cattaatgaa gaagcaaata caactgataa
    17461 aacaataata cttattaata ttaaataact ttgtccaagt gttgttatat ttgacataaa
    17521 actactatta tttattaaac ttccaccgga aatcattgaa tcctctgagt taatgaatcc
    17581 atctacaatt acttttagta atacatttcc taaaaataag gaagatatta tacttggtat
    17641 cgatatgaat attaactcga atataaattg cattataatt tgtatcttag atgttccaat
    17701 agataagaat atacctattt cataaattct ttcccttaac catagaatca agattaatga
    17761 aagaacaacc atcccaccta acataataga ataagtcatt acttttatta tatattttat
    17821 tccactcact gactctaaag actcttcaaa cgcattagaa tctttttcaa caaaatactt
    17881 tgactcatca attttaagct ctttcaattt gtttaaggct aagtctgtag attctgcact
    17941 accagaatac attaaaattt tatttgcaat ttcattattt tctgaattat ttaatatctc
    18001 ttggcttgtt gaataatcta caaacaccat attttcacta aaatcagaag ataatcctgt
    18061 atatgtttcc tgttttttac cagaaaagat ccctataatt ttaaatttat gactttttat
    18121 ttttccactt ttttcaatat ctagtaattc aagatcaact tcatcaccta attttagatt
    18181 gttttgttta gcaaattctt catgaacaat aattgaattc ttatcatttt cttctatatt
    18241 tttaccttct ttaattgtaa atactccact actaaataaa atatttcttt tagtattatt
    18301 tgtagcttcg agtgaaacaa cattcttaaa ttcgtcagat aaatcttctc tatttattct
    18361 ttgttctccg ctaactactt tagcatcttt tagttttgct aatccatcat attgaattat
    18421 tttttcttca atttctttta atttttcaat atctttaaat tgattaacat taaaatattt
    18481 accatctttt tttgttattg atattgaaga attagaactt tcatataaag ccttttctat
    18541 ttcattgctt gatttcatta ttgttaaaca agaatacaag caagaaagaa ctatagttaa
    18601 tatgataaaa attattaaag ttctattttt ctttcttgta atatatgcta tagcattttt
    18661 tatcactttc ctaactactc ctctctattt tcttactcca taactaattg catccactgg
    18721 acaagcattt ttacatcgtc cacatctgat acactccaga tgattgcaat tctcaactga
    18781 atctttattc atttggcaaa cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DACEDO010000060        17400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEDO010000060 DACEDO010000000
VERSION     DACEDO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.060"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(307..1440)
                     /locus_tag="IWO52_002105"
     CDS             complement(307..1440)
                     /locus_tag="IWO52_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0356358.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(1431..2099)
                     /locus_tag="IWO52_002106"
     CDS             complement(1431..2099)
                     /locus_tag="IWO52_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0356359.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="IWO52_002107"
     CDS             complement(2555..3541)
                     /gene="lacD"
                     /locus_tag="IWO52_002107"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0356360.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="IWO52_002108"
     CDS             complement(3554..4480)
                     /gene="lacC"
                     /locus_tag="IWO52_002108"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ0356361.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="IWO52_002109"
     CDS             complement(4493..5008)
                     /gene="lacB"
                     /locus_tag="IWO52_002109"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ0356362.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="IWO52_002110"
     CDS             complement(5027..5455)
                     /gene="lacA"
                     /locus_tag="IWO52_002110"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ0356363.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5475..6248)
                     /locus_tag="IWO52_002111"
     CDS             complement(5475..6248)
                     /locus_tag="IWO52_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0356364.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            6413..6892
                     /locus_tag="IWO52_002112"
     CDS             6413..6892
                     /locus_tag="IWO52_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0356365.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6949..7251
                     /locus_tag="IWO52_002113"
     CDS             6949..7251
                     /locus_tag="IWO52_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0356366.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7273..8730
                     /locus_tag="IWO52_002114"
     CDS             7273..8730
                     /locus_tag="IWO52_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAQ0356367.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9323..10075)
                     /locus_tag="IWO52_002115"
     CDS             complement(9323..10075)
                     /locus_tag="IWO52_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0356368.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10290..10526)
                     /locus_tag="IWO52_002116"
                     /pseudo
     CDS             complement(10290..10526)
                     /locus_tag="IWO52_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(10536..11500)
                     /locus_tag="IWO52_002117"
                     /pseudo
     CDS             complement(10536..11500)
                     /locus_tag="IWO52_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(11706..13388)
                     /locus_tag="IWO52_002118"
     CDS             complement(11706..13388)
                     /locus_tag="IWO52_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0356369.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(13399..13722)
                     /locus_tag="IWO52_002119"
     CDS             complement(13399..13722)
                     /locus_tag="IWO52_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0356370.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(13745..15151)
                     /gene="lacG"
                     /locus_tag="IWO52_002120"
     CDS             complement(13745..15151)
                     /gene="lacG"
                     /locus_tag="IWO52_002120"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0356371.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            15618..16625
                     /locus_tag="IWO52_002121"
     CDS             15618..16625
                     /locus_tag="IWO52_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0356372.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(17019..>17400)
                     /locus_tag="IWO52_002122"
     CDS             complement(17019..>17400)
                     /locus_tag="IWO52_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAQ0356373.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5218 a         3674 c         2860 g         5648 t
ORIGIN      
        1 acggttgtgt aaaaatatca ttttagagca tgaaaaaaag agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacatt tttgatcctt tttatttgat taattatatc
      121 cagctggata ataattttgg gggcaacatt tctatcttac tattatcttc ctaaagtagt
      181 agactttctt attgggaaaa attacaacaa aaaggagtgg cttagttgac aactcctttt
      241 tgtttacaaa tcaattggat atctataaat tgtattttct aacgaatttt atagacatct
      301 gatttgtcaa attaagctag acaatatgtc tttatctatg cagctcaaaa atacttccaa
      361 tggtgttcga taatttaatg attttcttgg aatatggttt cttttcgagg caacggattg
      421 aataaaggtt tcatccgtgt ccctaaagtc cattgtttta ggtaagccat cctgcttgag
      481 cccatgcact cggtactgat gagaaaaaat tgatgcctgt ttctcctgag gaatgccaat
      541 tccctgatct tttacttcaa taatcacgct acccttttgg tttgaaatgg tgacaattac
      601 cagttttcct tcaccatatt tgattgcatt gtcaactaaa ttcgaaaccg cccgataaat
      661 ccaacgatct cgtccatgaa tcagggttgg ttgatcttca gtaaacgcaa acaaaagatt
      721 cggataattc attttgtagg catcacaaac ttccgcacag atcagtgaaa cgtctacgac
      781 ttccgtgcta agatcctctg agttattgga taatgtcaaa atatcgtcaa tactgtcggt
      841 catccgatct aaaagcatta aatttccttg atcggcatgt tgccgttcta tttccagatt
      901 cgtacgcaaa atagcgatgg catttttctg ctcgtgggtc agataactat ttaatcgtcg
      961 ataatcctcc aaatgaccat cgaacttatc ctttagctta ttaaaagccg catttaatgc
     1021 ctcgtccttc acagtatttt taggaacttg atcaataaac gttaaatcac gcgtaatcgc
     1081 taagatttga ttactgtaaa ttttgcgtaa aaccaaccaa agaataaacg ttaataacat
     1141 gacggcagaa caaataacca gcaccatcgc tggtaaatat ttaaatagtt cgtcctggta
     1201 agctttatct atcatcgaaa tagtcataaa ttgattgttg gaagatttcg aagcttccat
     1261 ctgcgtctct gcttgaaccg tcttaccctc ttccataaaa taaacatctt cacctaaggt
     1321 agatgcgata ctgattccct ccaaattatt tctcagaaag aagaaactca ctaaaatcac
     1381 gcctgtcacc aacagtgaaa aagccagcat cgttaacgtc attttaaaat ttaatagcat
     1441 agctggtacc cctttgctcg aatggttttt aagacctctt tttcagctgc ttcagccagt
     1501 ttctttttta gacgagccag atgcactcgc agaaccgacg aaaaaggatc aaaatcttca
     1561 tcgtagacat gttcagaaat ttcctcagac gaaacaaccg ctggatgacg ctgagcgatg
     1621 cacagtaaga tatcaaattc cttgggtttc aggaccacct ctgtcttttg atagctgacc
     1681 cttcgaccta atggatcaat cttcaaagca ccaattgaaa tctgcggcag cgcacgtcca
     1741 tgataacgac gaataaccgc gcgtattcgt gcttttaatt ctagtaactc aaagggtttg
     1801 actaaataat catctgctcc aaaatccaag ccttttgctc gttcttcaat ctcatcacgt
     1861 gcagtaataa tgataactgg actttcgact tcttcagcac ggagactttg taaaacagat
     1921 aatccatctt ttttaggcag atttaaatct agtagtatcc catcgtatga attgatgaag
     1981 gccttctctt ctcctgactc gccatcataa gctacatcta cagaaatatt ctcctgctct
     2041 aggccctttt ttacggataa aaccagctca cgattgtcct caaccaccag tacacgcatg
     2101 tatattcact cctcatacgg atttagtaat tacacttatg ctttttagca tagtacacgc
     2161 tcttcttcaa agtccacgtt ctttttgtga agatttgaag aaattcatac attctgttaa
     2221 aaaacacata attaagaatg attttactgt ttgaaaatac ctagaataga aaaagactgg
     2281 aacctttcct ccagtctcac tactttctat ttgcttggtt ttcttgtata tacttttcaa
     2341 agttcgttgg tttatgacca ataagcctcg ttagaatact gaaaattgcc atcaagtcat
     2401 taagtaccac atgtaagaaa atctctctaa agaaaaaccc cttcgtttta actcacttac
     2461 tattttagaa ttcgttcccc taataatgca gcatgatttt acgctttatg tataaaaaaa
     2521 gactgcaagc aattttgctt cacagtctga tttcttatga tagtgagctt gttaacactt
     2581 ccaccggtgt agcagtttct ttcaatacgg catttaattc ttcaatattt ttacgccctt
     2641 gcgtattcat ccactccatc gctgcttctt ttccatcaac gatatagctt tctacaccat
     2701 ctgcccaagt cgcacggccg caaagtacac cattgaaggt tgagccggca tttttcgcga
     2761 agcgcagtgt ttcttggaac aactctgctt ttacaccggc gcttaggaaa ataaatggca
     2821 ggttagtggc ttcagcctgt tccttgaaga agttagccgc ttgtgccttt gtgtacacga
     2881 cttcgtctgt cccatagcct tccacataat tcatattgac gggtacttcc acctttaaca
     2941 catctacgcc ataacgtgga tcagaaaatt ccttcatcat atcgttgact ttatgaggtt
     3001 tcttaatggc gtattctttc gaagtcgcat cagagatgtt cgcgtcataa gaaacaagct
     3061 ccaagaagaa gggtaagttt tcagcctgac attctgagcc gattcgttca ataaaggcat
     3121 gtttttgatt attgatttct tctgcttcat cgacatcata gtaaagtagg aacttacaag
     3181 catcggcgcc tgcttctttt aaacgtttaa ctgaccagat cgataataaa tctggtaaac
     3241 ggccgggagt tgacgcatcg taaccagttt tttcataagc taacagcaag ccggcatttt
     3301 ttgctctaac gtcagctgct ggcaagccat attcgggatc taataaaatc gatgaggcat
     3361 atttcgttaa ttgtgaagaa acaatttctt taaattcaac gatttctgat tcttctgctt
     3421 cgctgccctt gaattttgtg atcatcttct tcaaagcccc gcgttgatca attgctaagg
     3481 ccccaatcac tccatcttca ttgcatagtt gcttgatata ttgacgtttt tcttcgttca
     3541 tttccgtatc ctcctacact ttctccacta agatttgatc atatagtttt tgataattgg
     3601 ctaattggac atagccagtc gtcgcttcct gagcattcag cattcctaaa acattaccct
     3661 gttttaacac ggcttcatcc gaaagacctt gttgaatcgc agcggtcaat ccggcaacgg
     3721 tcgcatcacc agagccaact ggattgacga catcaatctt tggaattgtc acgcaataaa
     3781 aagtctcgtg atgtttggca aaggctccgt cgccccccat tgaaaccact atccattcaa
     3841 tttcatcaaa catcgaatct gacaaggcgg cttttaaatc tgataattct gttgagattt
     3901 ctcgtcctag cagaccagcc agctcttcgg tattgggttt tattagctta ggctttacac
     3961 tgcctcgcaa cacttccctt aatgctgtac ctgaacaatc taaaacgaca ggctttggct
     4021 gacaaacttg cagcatttgt cgataaaaat cagcaggcaa tccttctggc aagcttcctg
     4081 aaaaagaaat gatttcggct ttaacagcta atttttcgaa atgttgcaag aacgcccttg
     4141 cttcttgttc agtaataaca ggtccgtttt ctaggatttc cgtctgtttt ccgtcatgta
     4201 aaacagcgat gcaattacgt gtctctccgc tgataggtaa aaagtcatga gcaatctcat
     4261 tttgagtcag gtccttttta atctcttcac ctaaaaaacc gccgattaaa ccactagcaa
     4321 tcacctccgt atctaattgc ttcaagatac gtgtcacatt taggccctta ccgccagctg
     4381 ttttgcgaac atttcttact cgattgactg tgtcgagctt gaaggtttct aatggataag
     4441 aaatatcaat cgatggattc atcgttattg ttaaaatcat ttgtccaccc gcttaatcgt
     4501 ggtattcgcc acgatcccat ttctcgataa attcatcgaa gaaatgcgga tcagactgct
     4561 tgtcactggc tttttcgacg tgatcgattt tggcgatcaa ttttttgttt tcttcagttt
     4621 cttgataatc ggcattgatg aaggcctcta atatgtcaca gatcaaaaat tctcccgtaa
     4681 tccgagcgcc aaagccgatc acattggcat ttagttgttc cttcgcatag atagcagtcg
     4741 tcatatcgcg aaccagcgca ctgcggatac caggtacttt attgacagcg ttgttaatcc
     4801 caacacctgt tccacacaaa caaactccta gatctgcttc tccgctagtc acggcctctc
     4861 cgactttttt tccgtagatc ggataatggg ttctggtaaa gtcataggtc cccatatcca
     4921 acacttcatg tccttttgat tttaagaaat ctgatactgc catttttgta tctgtgacga
     4981 tgtggtcaca gccaattgcg attttcattt cattcatcct tcctaattac gccattttat
     5041 ttaacatgtc gacacgtact tgatgacgac caccatcata atttgcttgt agaaattcct
     5101 taacaatatt caacgccaac tcatcgccaa caatttttga tccaagtgtg atcatccgag
     5161 aattattatg tttacgggtc atatacgcac tgcgctcatc cgaaacttct gctgctacca
     5221 ttcctttaac ctttgttgcc gtgataaagc tgccagcgcc gtagccatca accgcgattc
     5281 ctaaactacc ttcgttttca ttcagatcat ttgcgatcgc caaagttgat tccacaaaat
     5341 cttctgaagc cgtttcactc ttatctacta ctgtaatacc ttctttgatc aattttttct
     5401 tgattaattc ctttaattcc atgccgtcac tgtctgaacc aattactaca cgcatttttg
     5461 tttcctccta ttttttactg aatgatttta gtgaattgct gataataact cttcagctcg
     5521 gctgaaagag tagaatccgt aaataatttg tcgatcttgc gcaaatcata gaaattataa
     5581 aaatcgctat ggtcgaattt gtttgcatct gaaaccaaac atttttctgc cgcattatct
     5641 aaaacaattt tttgcaaggt accttcttca atactgaaat tcgaaacctc cgaacccttc
     5701 aatccattca ctccgacaaa agccttctta ataccgattt tttgaacagc ttcattggca
     5761 attgaaccga caaaggctcc agtcttttcc cgatatagcc caccgatcaa atacagttca
     5821 aatcttttta tttcacttaa tagattgaaa accggcagac tattggtaac gattcggaga
     5881 gatgacggct tgatgtatcg tgtcattaat tcgatcgttg tacccgatcc aagaaaaatc
     5941 gtgtcgtctt cttgattgat atgagctgcc gcatttttag cgatgtattc cttctctttt
     6001 gtatgcagct tctttttttc agtatgagaa agctcatttg cttctataga agtcgacatt
     6061 tccagataat cgattttttg tgcaccgcca tgaacgcgta aaagcttttt cttctgcgcg
     6121 agttcctcta gatctcggcg gatcgtcata tctgaaacat tcagtacttc ctgaataaat
     6181 ttgactgtta tcgtcccata gcgatcacac atcgctaaga tcgaacgctg tcgttctgtt
     6241 ttcagcatat tctgtacctc cacgacctta taataaaaca tttacgaaca tttttcaaca
     6301 catattgttc gtttattttc gtttatgcgt tgacaatgat tttgtaagcg gttatactca
     6361 acttgtaaac atataaataa acataaacga actaataaag gagctgtttt gtgtgaatga
     6421 attctttttc aaagacactg tctatgtttc tgacaagcag tcgcaggaag aagtgttcga
     6481 ggaagtctat caagatctgt taaagaaaga attggtaacc gaagattttt tggagaacct
     6541 tttagaacgg gagcgtaatt atccgacggg attagatctc tcacctgtct ctaaaatatt
     6601 gccagatatc gctatccctc ataccgaaag tgagtttgtg cgaaccaccc gtatcattcc
     6661 aatcaaagta aaaaatgaaa tccctttcta caacatgatc tcgccaagcg atcagatttc
     6721 cgcttcattt cttttcatga tcttgaacga aaatggtgaa gcacaggctg gcatgttggc
     6781 taatatcatg gatttcatta attctgttga cgaagaaaaa ctgttttcgt ttttcaaatt
     6841 agaggacaca gaagcgattt atcatttttt agaagaacat ttttatatat agaagtctct
     6901 gcctaaccgc aggataaaaa ataaacttat tttaagggga gaattattat gatcaaaatt
     6961 ttagcagcat gtggagcagg cgttaattca agccatcaaa tcaaaagcgc tttggaaact
     7021 gaattgaaaa atcgtggcta tcaagtcacc tgtgacgccg ttatgatcaa agatatcaat
     7081 gaagaaatgc tgtctcatta tgacattttc gcacaaattg ccaacaccga cttgggtttt
     7141 gacattaaca tccctgtcgt tgatgcaggt gctattcttt atcgtattcc tgctatggct
     7201 gagcctgttt accaaagtat ggaaagcgca atccaatcat taaacaaata ggactaaaga
     7261 aaggaaaagc atatgaatac aattattgat attgccaata aagtatttac ccccctaatt
     7321 aatttaggtg cggcaccgtt aatgaccatc gtattaactg ttattgcttt attttttaaa
     7381 gtaaaaccgt cacgcgctct tgaaggcggg ctcaaattag ggatcgccct gactgggatc
     7441 ggagctatta ttggaatttt aactactgct ttttcagacg ctatgacagc ttttgttgaa
     7501 cgcacaggta tttcattaaa cattacagat gtcggttggg ccccactagc aaccatcaca
     7561 tgggggtcgc catacacgct ttatttttta ctaattatgg tgatcgtcaa tattgtcatg
     7621 ttagcaatga aaaaaaccaa cacattagat gtggatattt tcgatatttg gcatttagca
     7681 attgttggtt tgttcgcgat cttctgcggt gctaatctgt ttattgcttc tgcattagtt
     7741 attttcattg gtgtattaaa aatttggaac tccgacttaa tgaaaccaac cttcaatgac
     7801 ttgttaaatg cgccagacag caacccgatg accacgactc atatgaacta catgatgaat
     7861 ccgattatta tggttttcga taaaattttc gacaaaatct tcccttggtt ggataaatat
     7921 gatttcgatg ctgccaaatt gaatagtaaa attggcttct ggggatcaaa attcgcgatt
     7981 ggaatttatt tggggatttt tgttggatta ctagcaggac aaacaccaac gcaaattttc
     8041 tcgctagcct ttaccgctgc cgtctgtttg gaattgttct ctttgatagg cgcttggttc
     8101 atcgcagccg tagaaccgtt atcacaaggg attaccgatt ttgccaataa acgactaaaa
     8161 ggacgtacca tcaacatagg tttagactgg cctttcatag caggccgcgc tgaaatctgg
     8221 gctgccgcta acgtattagc accaatcatg ttgctagaag ccatcatctt accaggaaat
     8281 aaattattgc cactaggcgg aatcatcgca atgggtgtta ccccagcact attggtcgta
     8341 actcgtggaa aattaatccg tatgatcgta atcggcgcga tcgaattacc attattctta
     8401 tggtccggca ccttgatcgc cccgttcgtt actcaaacag ctaaagcagt cggagccttt
     8461 ccagccggct tgaacgggtc cgccatgatc tctcatacaa cgatggaagg cccgattgaa
     8521 aaattcctag gttatttagt cggtaatgct tctcaaggac aaatcgaatt tgtgatatac
     8581 gctgccttag cattaggtgc atacctttta atcttcattt ggtacgctcg tcaaatgaaa
     8641 aaacgcaacg ctgcatacgc agctgaaaaa gaagagaatg catctccggc aacggcgcat
     8701 aatatgactt atgcagccga agctaaataa gctaaaaaca ccccgaatgt ctttagactt
     8761 cggggtatta ttttacctat aaaaaataaa tagatgtaaa atccatttca aaaagcatag
     8821 cccatctctt gccacaccca ataaagaaat tatcaacgtt tatgcttttt ttaactcctt
     8881 taagcttgcc tttgttggta ttcctcaacg attctttttg tgttcttgtt tgttttgtgc
     8941 tttttgactt tttgttgttt aattgcgaat tccttgattt cttttttcct gaatcgcttc
     9001 gcatcccgcg gttatgcgtg cgatttcatc attgcttttt ctgatctaac tcgtttcatg
     9061 tcaaagagct tcagctgact tagtgtccat ggttgttttg atatcccttt tcctccttaa
     9121 taaagcggtg attgatactg ctattttttc cttcacataa acataacatc ccaaaaaaat
     9181 cgactatctt ttttcaaatg gtgtattcaa gtattcggag gaattagtaa tgatgcaccc
     9241 taaaaataac ataattcgct taaattcaag gctaccaaaa aactaccaag ttctaattat
     9301 ctagagtttg gcagtttctc atttacacaa catatttttc ttctttttcg attttttcat
     9361 agtgcttttg ttcatcatca tccgttataa ctactgttac atcattcaga ttataatata
     9421 ctgaaaaatc tcttttccca attttcgaat gatcaatcaa taaaaagcgt tcaacagaat
     9481 tattcagagc gatctgttga gtttggccct cttcaaaagt cgaagtcata attttctgtt
     9541 catttaaagc attacagctg aaaaatgctt tatgaaaatt catatcctgt agcgatttat
     9601 tcgtgatttc accaacaaag cattgagtcg tttcccgcat ctcgccacct aatagataga
     9661 tttttcgatt cccttttcgc ttatttaatt cttcaaaaac aggcaaacag tttgtaacaa
     9721 tccgcagtga atccccttga atgatttttg acatgagctc gattgtagtt cctggtccaa
     9781 gaaatatcgt atcaccatca ttgatcattt cattcgcctt ttccacaatc cgctgctttt
     9841 cttcttgcag cataatctgt ttttctctat gagaaagctc cacatgccga taaaaatcct
     9901 tcaatttcgc tccgccatgg acgcgttcta ataatccttg tttctccatt tcgtccaaat
     9961 cacgtcggac tgtcatatcc gaaaccgaaa acttctccat aatatcgctg actcgtactg
    10021 taccgttaac ctctaataac gacatgatcg cttggaatcg atccgttttt aacatttcat
    10081 cgcctctttt ctttttaaga ctatcaaatt cttaagaaaa gtgctagaac aagttttctt
    10141 aagaagcctt ctgcaggaag gaaagcaaaa tttttcagaa tttgcaagct gctattctaa
    10201 aaaataaaac cgaacgagca tgactgtcat agtgacagcc ttgttcattc gacttttatc
    10261 tttaataaat cattttattt aatctgaagt tattgtgcaa gttcaaaaac aaagtagtta
    10321 atgtctcctc gatatttcga aatcgaatac tcaacgggca gacgttctag cgaaaaaccc
    10381 tgtgtatgga aatagaacat tggatctcct tcttccacat ctaacaaatc gctgatcgta
    10441 tcatccgctt tgattacttc tagttttctg gtgaccgttt taattggata acccatttct
    10501 tcaaaaactt gatacatttt ttcttttctg atttgtcaaa ttaagctaga caatatgtct
    10561 ttatctatgc agctcaaaaa tacttccaat ggtgttcgat aatttaatga ttttcttgga
    10621 atatggtttc ttttcgaggc aacggattga ataaaggttt catccatgtc cctaaagtcc
    10681 attgttttag gtaagccatc tcgtctaagc aagccgtttg agtgctcgtg ctcatttagc
    10741 cctctttgcg aaggtgttcc tggatctgcg aaaaagatgg caatatcgtt cgagttactt
    10801 atagatcgcc agttggaaaa ttcttttcca caatcaaatg tcatggattt aaacaagtgt
    10861 ttaggcatgg atttgaacca atcatttaac ctgttttcga tatcttcggc tcgtctgcct
    10921 gtcggtttca aagtaataat aaccttggat aaacactcaa ccaatgtaat aacagcgctc
    10981 ttgtgtttaa ctcccacaat ggtgtcgcct tcgagatgac caaattcttt agtcaggtgt
    11041 ggatagagac tgttccgttc atgaatcgtt ctgcgaaagg cctgttttcc acgcttttct
    11101 tgatgattat tcggtttacg tttccccttc ataggtaacg actgtgtgtc aaaaaggcct
    11161 tttttaaaca tccgatagag tgtacgaaca gaacaatcaa ttggaaactc gccacggccg
    11221 ataatcacat cgggtgacca cccttgagct accttttctt gaacatatgt tgattgttca
    11281 tcagataaaa cggttgaacg tcgtccacaa cgagatttat tttgtttgta ttgacggtaa
    11341 taatctagca cttttttcct tgtttgagaa acgaataaac attataaatg gtttgtctag
    11401 agcgatttaa actttgtgcg acaagagcga caggtttctc gatattgaaa taagactcta
    11461 tgattactag ttcgtctgtt gtaagatggg tgtaggtcat ttgtactcac tccttattaa
    11521 ttctttggtc ggaactattt tgagtgtagc acaaatgatc tttttagttg tctagcttaa
    11581 ttttacaatc gacgcctcaa aaagaatacc cgaaatttaa acagcaaaaa aagatagcgc
    11641 ttaccattgt gtttaagata aaacgctagc taagttggct agcgctttat taatctaagc
    11701 aagctctaaa attgcttcaa tacaaaatca actgctcctt taggatcacg tgttaaattg
    11761 atatattctg cacctttagt tgcaattact tttatcccta atttgtctgc atctttacgg
    11821 agatcatcca aataggaatt cacttgaggt gctagaacga ttaaatcaaa atttttcatc
    11881 atatcataat gagaaccata agctcctgct ttagcagaaa gattaacacc tgtctctttt
    11941 gcgccctctt ctaatgcatt agccaacatt gcactcgttc cggcaccagc acaaagtact
    12001 aaaacgcgct ttccttcacc aaccgatgct tttgcttcat caactataac tgccgcagtt
    12061 gcaccaactt cttggatttc ttcgaccaat tcttctttta cttcttctgc agcaatgttt
    12121 tttaactctt gttctactaa ttcagaatca taggccttaa caaaaggtaa ataaataatc
    12181 gcatcgacaa caatcaatac taccgctaat acaatcgcca acacactaaa cccggttcct
    12241 aaaaccaagc caatcggtgc gggcgtcgcc caaggcaata cggatataaa gctattcatc
    12301 cccaagacat cgacaaaaag tttgaatatc catacgttag cgattggtgc taataaaaat
    12361 ggaattagaa agtacggatt caaaataata ggagtagcaa atagtaacgg ttcatttacc
    12421 gcaaacgaaa ctggaataaa tgaagcttta ccaactgctt tcaattgttt tgatttagcg
    12481 aacaacatga acagaaaagg aacaactagt gtcgctccag taccacccat cgttccaaca
    12541 aagtttccta gaccaactgt taatactttt gaggcttgtg caccgttttt aaataattca
    12601 aagttagttt ctacgttcgc ataaataatt gcagcaatcg ctggttcaac aatcgatggt
    12661 ccatgaacac caacaaacca gaacattgcc atggctcccc aaatgattgc aataccgaca
    12721 tatccatcag cagcactaaa taatggctgc aataattgaa ttacacctgc tgcaaatgat
    12781 gttccaaaaa attgacgaac tcccgtatca atcaaaacgg caataaatac tgcaattgaa
    12841 aaggggataa tatctctaaa catctgtgtg attgttccgg gaacttcttt tggcatacgg
    12901 atcgtcaaat ctcttttaac acagaattta tacacattta cagtaacaaa cgcagaaacg
    12961 aaagctgcta ataatccctt tgtccctaaa taatcagtag ctatcccacc atcaatttga
    13021 tcagctgcca tcattacaaa accacaaatt gacgctaaca taacagaaac ttcattaatc
    13081 tgcttacctg aatcagataa ttgacggttc attgaattag ctaaacatcg agctgttgta
    13141 gcaacaacca aaagaccaac taatcccatt gaataaccat aaactttcca aagccacgtc
    13201 gagaatgcct ctggtaacgt cacgttaaat acttctggaa cagccgcaat cataataaaa
    13261 atacttgaaa atagaatagc tggcatagca gttaaaaagc catcgcgaat tgcccctaaa
    13321 taaatattac gtgagatttt ttcaaaaaac ggttttccct tctcaatttg cttaataatc
    13381 gtattcattt ttcttctcct atgctttagc aaatttttct ttgtacaatt caacaaaatc
    13441 attgataagt tctcttaata gaatcgttgt cattaaatgg tcttgcccat gaataaaaat
    13501 tacgcctact tccatttcgt tccctgacgc ttcttcagct aaacatttcg tttgtgagtt
    13561 atgcgctaag gttaaatttt catcagcatc cgctaataat cgttcaacat tatcaaaatt
    13621 accttctctc acttccttca acactgttaa taatgaactt ctggcatctc ctgcataagc
    13681 aacaatttca aatcctaata attgcaattc tttcctatcc atttccttgc cctcctaact
    13741 atttttatat tatcgttttt gtttcgctaa caaatttata ccaatatgca gactctttcg
    13801 gatatctttt ttgtgtatcg aaatctacat agaatagtcc ataacgctta gtgtatccat
    13861 taacccaaga gaaaacatcc attaatgacc aaatgaaata cccctttaca ttaactccca
    13921 ttttgatagc atcggaaatc gcattaaaat gctggcgtaa ataatcgatt cgatcatcat
    13981 ctagaataat cccatcttca aaattatcct tataccctag cccgttttca gtaatgtata
    14041 ttttattata atttggatat tcgtcttgga ttcgtacaag taaatcatat aacccttttg
    14101 gataaatgat ccaatcccaa tcggttttcg gaagatcctc acgcacgatt ctctcgccaa
    14161 tccctttaat gcaatagact tggctacctt tttctccggt agtgttgtag tgaagtgtgc
    14221 tttctccttt gtactcttta acccaatggc taccatagtt attgatacca agaaaatcat
    14281 ttcgatttgc tgcttttttc atctctctaa aatcattaga atcaatatct gcagattgcc
    14341 catttgctgc taaaatctca ttaatatgtg ccattgtttc atctgagtaa taccctaaat
    14401 aagtagcatc taataagaac ttaattgata acgcatcatc taatttcgca gcatgcttat
    14461 cttcttcttt gtcacttgct ggatattttg tctctagcga gtgaacaaca ccaatttccc
    14521 ctttatatcc accatcttta aaaatgttta ctgtctttgc gtgcgcatac atcatgttgt
    14581 gtaaacaagt tacagtcttc tctatatcaa attgaacttc tggaggaaac gatccgatca
    14641 aatattgatt tgaagcaact ggataaatct cattaaatgt actccaataa tgaacctcag
    14701 aaaattcatc gaaacagaac tgtgcatagt ttacgaaatg ttcaacattt tctcgattta
    14761 aaaaatctcc attatcaaat agttctttag gggtatcaaa atgatgaaga gttacaaaag
    14821 gaataatacc atgcttattc aattcagcaa ataccttatg ataatattcc actcctgttt
    14881 ggttcacttc cccatagcct tttggaaaaa tacggctcca tgcaatagac atcctaatac
    14941 tatcaatttt aaattttttg cataattcag catcaactgg atattgatta taaaaatcac
    15001 tagctggatc agcagtatag cgtccctttt ctacaaggaa agtatcccac gctacttgcc
    15061 cttttccacc ttcttttgta gagccttctg cttgataagc tgccgtagct cctcccataa
    15121 taaagtcttc tggtaaagtt ttataattca ttttaatctc tccttgaaaa ttagtttgat
    15181 caatatgcca ataatccata aatcacctca gcataatttt aacatgtata agtaaatttt
    15241 tttacacaac gaaaaaccga cctcgaatat ctattttatt gcgatataca agcacttata
    15301 tcccttgatg taaaataaat ataccattca aaaaccgttt tgtaaacgtt tttttgtaaa
    15361 ataaatttat ctttttacta atttaattta cacgaaacaa gaaaacggac tgattcccac
    15421 taaaatcaat cccacctact aatcattctc caagtggcta tccattatcg attcaatctt
    15481 ctctgttaat ggattaggct ctctgctgat atcagagttt cgtttcctgc tagaaaaagt
    15541 aatttaagtt actaaaagag cagtttcgat ttactttaac aaagaaaagg attattatat
    15601 ccctaggagg atgaacaatg gttactatta gagatattgc aaaaaaatct ggttactcaa
    15661 cggctactat atcaagactt tttaaaggtg atgagtcact ctctattaca tctgatacta
    15721 aaaacaaaat aatcgttaca gccaaatctt taggatatga tctgacaaaa attaagacta
    15781 ctctgtacaa gattgccatt cttttttgga tctcagagga acagttaaaa aaggatgtct
    15841 actatcaaca tgtcaaagat gatttgaaaa attatagtaa aaaagctaat ctagatttga
    15901 ttttcatctc taaaaacgat ggtgtaaaaa gcattcctga cgatattact ggatttatcg
    15961 caataggaca attgacaaaa aaggaattgt actacttaaa tcaaaaaggg tataaggggg
    16021 taatccgagg aataaaccca ttacctgaac acttcgatac tgtagaacct gatttccacg
    16081 aaatggccag acaagcaatt aactacttta ttaatgaagg tttcacaaaa attggattta
    16141 ttggaggaaa tttctttaac ctagaaacgg gtaaagaaga gattgatagc cgtgaatatg
    16201 ccttccgata tcatttagaa aagagcggac tactcaatga aaagtatatt ttctcccaag
    16261 gatcatttac tgttgacgaa gggtatcaat taactcttaa aatgaaagct gagttaaatg
    16321 aagatatgcc cgaggcatgt ttcataccat ctgacactat agctgccggc gttcttcagg
    16381 gctgccatga attgggtatt aacatcccta aagatatggc agtaataagt attaatgatg
    16441 acgacatcgc aaaattcgtt gcaccaccac ttacaacttt taagatagat atcgaagaga
    16501 tagctaaaac ctgtgtagat ttattaagtg atcaactggt atattcgcgg gacatatcaa
    16561 agaaaacatt aatcggcgca aaattgaccg ttaggaaaag ttttatccct aaagaagagg
    16621 tgtaaaataa gtaattgaaa cttatcttta aaactataat tgtgtccaca tagtagccga
    16681 gcaatcgggt ttatgcctat tatgttttcc ataaataatt tgatggtgat attttgacgt
    16741 aaatacaata tgattttttc atcgccaaat tgtgcgcgct aatttttgtt tatcattcag
    16801 aacatttcct cctttttatc taagtatgag gttggataaa cgcttcatct tagtatcttg
    16861 ggggattttt tatctcgttg ggaatatatc aagcatcaga gcgcgcaaaa agaactggtc
    16921 gaaaaatcaa tctaccagtt caatctcatt cactgtgata ctatgtttta tacagataac
    16981 gccatattat aaaacaagtt gtattccgca aatgggttct attcaactct taaactatct
    17041 ataaaagatt cagccaactc cattagcctc tcgtaattta gttggtgaac agcacttttt
    17101 tcaaaaaatt ttgaaccaat tcctacataa gaggctccat tgttcaaata gttttgtgca
    17161 ttagtctgat ctacccctcc tacagccatc aaaggtaaac ttcctaaagg ggcttgcact
    17221 tgattgaaat attccggtcc aagacagttg gcggggaaca ccttaacaat atcagcaccc
    17281 atctcaaaca ttttgaatat ctctgtgggc gacatggccc caggaattgt tagaaggttc
    17341 tgttgcgaag tttgaaaatc aaacgcgccc tctctgaact ccaaatatct taggctgtta
//
LOCUS       DACEDO010000061        16818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEDO010000061 DACEDO010000000
VERSION     DACEDO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.061"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..454)
                     /locus_tag="IWO52_002123"
     CDS             complement(<1..454)
                     /locus_tag="IWO52_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0356374.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(559..1422)
                     /locus_tag="IWO52_002124"
     CDS             complement(559..1422)
                     /locus_tag="IWO52_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0356375.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1618..3717)
                     /locus_tag="IWO52_002125"
     CDS             complement(1618..3717)
                     /locus_tag="IWO52_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0356376.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="IWO52_002126"
     CDS             complement(3937..5046)
                     /gene="rpoD"
                     /locus_tag="IWO52_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0356377.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5070..6911)
                     /locus_tag="IWO52_002127"
     CDS             complement(5070..6911)
                     /locus_tag="IWO52_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0356378.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7093..9486)
                     /locus_tag="IWO52_002128"
     CDS             complement(7093..9486)
                     /locus_tag="IWO52_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0356379.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9593..10453)
                     /locus_tag="IWO52_002129"
     CDS             complement(9593..10453)
                     /locus_tag="IWO52_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0356380.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10512..11033)
                     /locus_tag="IWO52_002130"
     CDS             complement(10512..11033)
                     /locus_tag="IWO52_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0356381.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11353..12072
                     /locus_tag="IWO52_002131"
     CDS             11353..12072
                     /locus_tag="IWO52_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0356382.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12098..14689
                     /gene="mprF"
                     /locus_tag="IWO52_002132"
     CDS             12098..14689
                     /gene="mprF"
                     /locus_tag="IWO52_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0356383.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="IWO52_002133"
     CDS             complement(14877..16064)
                     /gene="tuf"
                     /locus_tag="IWO52_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0356384.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16432..16788
                     /locus_tag="IWO52_002134"
     CDS             16432..16788
                     /locus_tag="IWO52_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0356385.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3491 c         2946 g         5764 t
ORIGIN      
        1 tttctttttc atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta
       61 aaaaatgata acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt
      121 cctcgatcgt gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt
      181 ttctcaaatc ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt
      241 ctgtcaagat ttcaagggtt cgataaacag tagcaagtcc aatttccgga ctcttttttt
      301 tcactaaaaa gaagatttct tctgcagata aatgctcttt ctcattttcc aaaagcacaa
      361 gcagagtagc ttctctttgc ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg
      421 tttttttcat cgccgaaata gcattcatga ccatttactc aaccccaaca atttataata
      481 attacaaata aaaaaagata taacactatg ttaattaaaa ttattataag ctagttaaga
      541 aaaacatgca agggattctc aattagattt tttgactagt tttgtcacac cgttttcttg
      601 tgtaatcaat ccttgtttca aaagattacc taatgcgcgc ttaaatgctc ctttgctgat
      661 accaaaagct ttcatgattt cttctggatc cgatttatct gtaaagttga tttgctgatc
      721 tgcagaacgt tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag cttcatatgc
      781 tcttggcttc aatgaaagat tcagtacacc gtctggtcgg acaccaatca ctcgaccttt
      841 tatctgctcg cctagacgag gttcattata tcgttcagaa ggatggataa acccaagata
      901 gaaatcttcc gttaaaacgt acgtacctgt taatttcaag cggtatgctg ttcctatcac
      961 atctttgttt ttcatttctt ctgttccata tttgcttaaa gatttgaata ttttttcatc
     1021 tgctaaactg ccccaaatac ggtcttttgc atcaactcta agggaaatca tcaagcgatc
     1081 ccctttttta ggccatagct ctgtcattgt cggcaattca tcaagagata cgacaaagtc
     1141 tttatctgga agaccgatat cgacaaacgc acctaaatct tttcgtgtag cagtaacgat
     1201 tccaaaagcg taatgaccga tacggctttt aggaatttgt gtagtgatcc gattttcatg
     1261 tttttgattt tggtaaccga atccttcaac tgcttcaccg attttgtgtt caccttctga
     1321 tttgcttaag ccaaaggttt gtccgttttt ttggacaaag tagttttttt cattttcatc
     1381 aataataata cctgtaaata tagttgctaa taaatcgttc atttttttct ccctctatag
     1441 tgaaaggttt tcttaataca tttctttgct atcataacat aatgttatct acttgaatag
     1501 ctgagccttg gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt catactcccc
     1561 tttttcgaat aaacggtgtt aaacggcagg ctcacagcct ctttcatttg cctaaaatta
     1621 attgctccca tttcctccga accatccagt tttacagttg atggttacga tgtaaaatgg
     1681 caaagcactt tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg catatagatt
     1741 ataatagcct tccccatttt cgattctggc tttttctaat ctgtaacctc cttcaacgat
     1801 atatccacgt tggatacatg tagcaatcac tttgctttgg acacctggtg cccacgtcca
     1861 tgctggatta gatgcatcgg caatatcact tgctctggca gatttgattg gctgattcgc
     1921 tgagtttgaa gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc
     1981 agcttgttga gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc
     2041 agctgctttt ttcgcttctt ctgctttttt cttttcctct tcttcttttt tctctttatc
     2101 agtgagcatc ttatctactt gtttcaattg ttcattcaac tgagcagata gatctgtatt
     2161 tttcactttt tctactgctt cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac
     2221 tacttttccg tctttaaaga ctatagctgt tttttcttta gctgtttgca attcagcata
     2281 ttgattttta gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt
     2341 ttcgattgct tggatctctt tatctgcttt caacttgatg tcatctgcta gatgatcatc
     2401 gacgatggca ggtgctatga accactcatt cactttttgg atatctcgga tcttagattg
     2461 gatatcttgg taagttgttt ctaactctga atatcctttt tcattcttaa gactggataa
     2521 atcatttttt agttttgata aatcttgatg aatcatatct gcacgaataa attgatggtg
     2581 atcatctgta taaaaggcag caagtttctc atgaagttct tccatttgtt gctgtctttt
     2641 tgcttctgct gctacttcgg cttctacttt ttgatgatgg tcataataag cccagcttcc
     2701 tccaccgagt agaagtactg ctgctaaaac gagataaggt actttttttg attttttctt
     2761 ttgcgcttta tctggatctg ttttttctaa gtttgattct tgatgtgttt tttcagtatc
     2821 tggcacgatt gttattgatt tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt
     2881 ttctattttt gcttcaagat cagtaccctc tttgcttaca gattgatttt cagcatcgga
     2941 ttcactattt atttcatcgg gctctgttga ctttttgtct gtgttttcga gtatttcttt
     3001 tttctctgct tccggcagtt tattcgtttt ttccttttct aattgttttt cagtaaatac
     3061 ttcagttttt tctgtgatat ccgtttttac gtcttttatt tttactgttt ctttacctgt
     3121 ggcgttttct ctatcagagt ctttttcagt ttcttgttcc ttttctggct ctactttgtt
     3181 ttcctgtgca attttttcac tgatcatttt gagcagctct ttcgcgtgct cttcttttgt
     3241 atcttcacga tgtgctttga tataagcgga tagtatcgaa ttttcttcct cattatcgac
     3301 gatttctttc agttcagcta ctcttctttt ttgttttgat tcaagcgttt cttcttgatt
     3361 ctttcctgat tcttccgaag tctcatgaac agttgctttc ttctgttcat ttttctctat
     3421 attagttact tgatcgtgag caggtggttc ttcgtcttct ggttctgctt tttcgaataa
     3481 ttccattaca gattctagag gtaaatcttt caattccgac cattcgattg gatcgtttaa
     3541 ttctgtatca ggaaagactg catcttcagt tgatttatcc tgcatagctg catctgtatt
     3601 tgtttcttca attgattctt ttatttgctg atcttctgca gttatttgat gaccgcagtc
     3661 tggacagata gtattctgct ccattttttt cgatccgcaa tttggacatt ttttcatcac
     3721 ttagcgatcg gctcctttac taggtttacg ctaccattta atctatcata ttctatacgt
     3781 cacgccaata gcttattttt aggatttact taaaaataat gaatgaattt ccttcctata
     3841 gaaaaaaaag cttgagactg cgtagtctca agcttttttt cttttaaaat aaaccaaaca
     3901 gttttactga tttctcttct ggtcaatagt ccacgcttat tctaagaaat cttttaattg
     3961 ttttgaacga gaaggatggc gcaattttct taatgcttta gcctcgatct gacgaatacg
     4021 ttcccgagta acaccaaata ctttccctac ttcttccaat gttctcgtcc ggccatcatc
     4081 taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat ctagtacatc
     4141 ttccaattgt tcttttagta actcataagc tgcgtgttct gctggacttg ttgcttcctg
     4201 gtcttctatg aaatcaccta agtgagagtc atcttcttca ccgattggcg tttcaaggga
     4261 aacaggttct tgcgcgattt tcaagatttc tcggactttt tctgttggaa gatccatttc
     4321 cgcaccgatt tcttctggcg taggttctct tccaaggtct tgcagcaatt gacgttggat
     4381 acgtatcaat ttattaattg tttcaaccat atgaactgga atacggatcg tacgtgcttg
     4441 gtcagcgatt gctcgagtga tggcttgacg aatccaccat gtagcatagg ttgaaaactt
     4501 aaatcctttg cggtagtcaa atttttcaac tgccttcatc agtcccatgt ttccttcttg
     4561 gatcaagtca aggaactgca tgcctcgtcc tacataacgt ttagcgatac ttactactaa
     4621 acgaaggttt gcttctgcca gacgctgttt cgcttcttgg tcgccttctt cgattttcaa
     4681 tgctaacgct acttcttctt ccgcagttaa aagggagaca cggccaattt ctttcagata
     4741 catacgaacc gggtcattga tttttacacc ggttggcgca gaaagatctt ctgtttttgc
     4801 ttgttcagct tttttctcag cactcttcaa gctgtgaatt gatggatcac cattttcatc
     4861 caccacactg atacctgcgt cttcaatctt ttggatcaat ttgtccattt catctgcatt
     4921 caaagtgaat ggtgtagcaa gctgattgga taaatcatca tatacaactt gtcgtttggg
     4981 tttgttttct ttgatgaaag ctgctactgc tgcttcatac gtttttttat ctgttccatt
     5041 tgccataaaa gaaggccccc cttgagtagc tatttggctt gttttaactg tttcgttaaa
     5101 ttgatgattt caatcgtcaa ttcaagctca agctgtttgt ttccagttcg tctggcttct
     5161 tgctgcattt gtttttttaa tgttatttca tcagctaagc cagatttgct gatcacatct
     5221 agcaagtctt ggatttctcg tggcgaactt tcttctgcca ccggtaaata ttcgatttcc
     5281 accaccaacc gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat
     5341 tcataatgtt gtgagacata tgcatcgaat aaaaaataaa cttctgcata tgcatcatga
     5401 ataaactgaa attctcgttc ttttaaaata ttacggacag aaacttcttt aaatgcacga
     5461 tataatagaa tctgctctgc cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt
     5521 tcttgtctcg taacctgagt gtgttgactt tcctgaggtt cttgacgatt ttgctgattt
     5581 gtccttcgaa gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg aaactctgtt
     5641 gacagttgag atagatacat atcttgttcg attggtgaaa caactcggct aagttcaact
     5701 agtaattctt cgaggtattc gatcttgtct ttttcattat ctaaattttt cccttgtttt
     5761 aaataacgtg atttaaaagt aaagactgtt tcgcgcccat gagccactaa ttcttggaaa
     5821 gatgcttctc catacttacg gagatattca tcaggatcaa gcttttctgg aacgactacg
     5881 acagaaagtg ctaatcgact gttttgccga agcaattcaa tccctcgatt ggttgcttca
     5941 aaacctgcgt tgtctccgtc atagcaaaag accaattctt tcgtcagctt ttccatttgt
     6001 tggatctgtt gagctgttag acttgtcccc atagaagcta atccagtttt gataccagcc
     6061 tgccatgcag caatcacatc cataaaccct tcgaacaaca aaatttctcc gtttttccgg
     6121 atatcgcttc ttgctttatc aagattaaat aaaacttgtc ttttattgaa cagttctgtt
     6181 tctggactat tcaagtattt gggctgatct ttttcctgcc ctttttcagg tgcaagccag
     6241 cgtcctgaaa aaccgatcgt ctggccttga gaattacgaa gtggaaacat cactctttga
     6301 taaaaacgat cagacatacg gccatcatca tgcgtgacaa acaatccaga gcgttctaac
     6361 aattcatttg aaattgattc attcttgaat acttgctgaa gaaaagaacg ctcattaggt
     6421 gcaaaaccta tgttgaattc ttcaattaat tctaaagaca acccacgact ttgcaaatat
     6481 tctaaagctg gctgtccagc ttttgtatgc aatagcatgt gatgatagat ttccgctgct
     6541 ttttcatgag cagcaatcag tttccccgta gcagattcag gattttggac agctatagta
     6601 cgctgacgcc attgatcttc taacggtatc tgttcgaagt ctgccacttt taccactgct
     6661 tctggaaaac ttagaccttc tagttcttga agaaaagtaa acacgtttcc gcctttacca
     6721 caaccaaagc aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct
     6781 tcatgaaaag gacacagccc caagtaattt ttaccagact ttttcaactg gacatattgt
     6841 ccaatcacct caacgatatt cgtttgactg cgtactgttt cgataacctc ttgaggaatc
     6901 cgttgagcca ttttttcacc ccctgctcta ttgatatctt ttgggtttaa cagaagatat
     6961 ttcaggagaa taaccggtaa aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca
     7021 caattataca tgtttgattg tatcatattt cattgctttt tcaagtataa cacatttcta
     7081 tctcattaag catcaatttg ctactctttt cagtaactga ttttgaatat cagccttcca
     7141 gtacaatact cgaagaacaa tatttacgag catgaataca aagaagtatc ctacgctctc
     7201 tcccaaccct aagtttactt ccaaaattcc atggaacagc attgcagcta catatatccc
     7261 tacgatcgtc gttactgcta ggaagatgcg caatgggttg aagggataac aagcttttac
     7321 aactgccatg caactgatcc caatcagcaa gtaatacatc aaagtagtag tttcagcatt
     7381 tccccatgcg aatgtttttc caagtagata caccgcaatg atattcacta ctacaagcaa
     7441 ggcatttggt aaagcattaa taagtgctgt aggcaaataa cggtaagtga tttttctttt
     7501 atcttgttca aaagataaaa agaaggaagg atacccttca atcgctaaat caatcagcgt
     7561 gatttggata gggataaacg gaaatccaat tgctgtaacg gcacatataa tcgataaaat
     7621 aaatgaataa atcgttttga taaaaaagat gcctgagact ttcgtcacat tgttgaccac
     7681 tcggcgtcct tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca acaataccag
     7741 attcgcaatc tgacgtgttg ctccatcacc ttctgccata gcaatgctgc aatctgcttc
     7801 tcttaacgcc aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc
     7861 tttcaattca tttaccagca atttcttttg ctgcggactc actcgtccaa aaaccgtata
     7921 gcgatgagca gcttctcgca cttccatttc ttcagtaatt tgagaaagat cgacatacgc
     7981 atcataattc ggtaatcctg cacgtctagc gatattggag acagtaaaag gattgtctcc
     8041 agaaatcact ttcagatcca caccttcctc atgaagataa gctagtgttt cttgagcgtt
     8101 ttttcggatt ggatcatcga tttcaaacag agcaaccggc tcaacaaatg ctggtttttc
     8161 ttcgttcaat tgctgatttg gagcgatccc caacataagt acgcgatagc cattcgcttg
     8221 ggctgttttt agactgtccg gaagccgatc ttcttccacc aaacgttcag gcgctcctac
     8281 gtacacgact cccagttctg gaaattccat cgcgccccat ttgcgatcag aagaaaatgc
     8341 gactttgttt gtcacatcat accgattaga aagctcaaaa taatctctca tagcccgcat
     8401 agtgatgttg ttatcactgc tttctgccag atacgtcccc ataatttctg ggatggcttt
     8461 ctcgtacagc ggatgtagtg cttctacccg ctggatactc attttccctt cagtaatggt
     8521 ccctgtttta tccaagcata atgtatcgac atgggccagc gtttcaattg aatacatgtc
     8581 ttgaacaagg attcgttttt ttgccagttt ggttacccct gtcgttaatg caatacttat
     8641 tagcaatacc aaaccctttg gaagcatgcc taacaaagct gctgcagatg caataacaga
     8701 aattttgata gaagcatcgc ggatgaacag tgcttccaaa aatagtatga tccccaaagg
     8761 aataatcacg aaactagtga atttggatac tttgcgaatc gaatctacaa gttcagaacg
     8821 aatagctttg tgagtctttg cctcgctagt caagcggaca gcataattat ctgcacctac
     8881 tcggataact tcagccatga cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc
     8941 tccagtttct ttgacaatca aatcagattc gcctgtcaaa agagcttcat ttgcttctgc
     9001 ccgtccagat ataacctgta aatcggctgg tatctgttct cctgctccga tttttaccaa
     9061 atctcctaat accagctctt tagtagaaat cgattgttct ttaccgtccc ggatcactag
     9121 tacttctgat tgggaaacaa tcgataattt ccgtacaaga tttcttgcat gtatttcttg
     9181 ataaattcca ataacgatat tgacgacaat aattgctaaa tacgccatgt tggaatacgc
     9241 acctacaaat aacagacaaa tcccaatacc aaagttcaga aaattgaata acgtcaatat
     9301 attatcgctg aatatttgtt ttgtactttt tgatgcatct tcctggtagt cattttgctg
     9361 gccagaagcc attttctgaa ctacctcttg ctgtgtaagt ccattcattt tctttttctc
     9421 cattttccgc caactttcaa aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa
     9481 aaacaacttg tttttataaa atttatagaa aaacacacga atgattttac aaaaaagatg
     9541 ccaacctttt tgtttgaatc acccgtgtgc tgtttttatt atcaatctat ctttacttat
     9601 attgttcctc caaacggctc atccaccaac ctagtgctgt accggctata aaaagaacag
     9661 gacaagctag taccccagca actcccaatc ccgcgtaatc ggtaggaatg atcataatcg
     9721 ttgaagcaaa aactacacct aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa
     9781 tgtaatccat gatttttgac aatccaagaa cacaaagaat cccaccaatt ccgatcggca
     9841 caatcacttc aagtcgtact tctttaaatc cgtcagccat cgctttatac aatcccatat
     9901 acaccaaaaa attagaaggg ctcaatcctg ggacgatcat acccaaacca atcaatccgc
     9961 cggcaatcag ccatgttcca aaattctgat cgacttgccc gtcaaataat tgagcgccgc
    10021 ataataaaaa caaataagcc agaacaaaag taacaaccat gatgatcaca tcagaagtat
    10081 ctcttccttt tttccctgct tctttccata acgcaggaac agttccagcg atacatccga
    10141 caaaaaacca taatatcgtt gtttcatacg cacctaataa aaaactgact aaaaaagaaa
    10201 gtagaaagat gcctgtcact ccgcctagac caacaggcag aaaaaacaaa acattttctt
    10261 tgaaattttt tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg ccaaagacag
    10321 ctgctaatgc accaccgctg actcctggca agataaatcc tgaaccgata aacattccct
    10381 tcacaaaccg taacatccag tctttggttg tgtaccccac ttcttgattt tccatttttt
    10441 gccttttttc cactatttgc gcactccttc tttcatttaa ttttattcat gattgaacat
    10501 ttcctgctat tttacttaaa gactttttca taggtctcta ttattttttc attcattttg
    10561 acaatatcag gataaaacat gttacctcca ttatgataga gatagccgcc attgtaaagc
    10621 aaggactgga tccgccaata tcggtattgt ttcccttcct catttcctaa aagtgggctc
    10681 agcacatctt tagaatacgc ttctgctaat tctactgtgt tttctccgcc atgatttttt
    10741 acataggcaa tataatcttt cccaaaatta tatgcttgga cagctgtcca taaatcgcat
    10801 ccttgtttct ttgcttctac tatcatttcc gccaaatatt tgacaccttg atcaatactg
    10861 tcagctggat tatcaatgat ctcagcttct ccatgcagac tttccgagct ctgcatgaga
    10921 tctgtccctt ttccttttga ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt
    10981 tccatatgat aattttttgc agctgttgtt atttctattt gatagctttc cactcgtttt
    11041 accgaacgat aagttcttcc tcataaaaac aacaccgcac caataaccgt cagaatgatc
    11101 atgtaaaaaa gaatacggat aatagataat cgttttcgct tcatttcatc acctagttat
    11161 tttgacatct ttttttatct tactatttct ttatcttctt ggctatttct tccttgattt
    11221 ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt ttgacatctt acgaaaatga
    11281 tcgttgtttt tcagtaaaaa caattgccca atgactgata taatcgttat actttacaca
    11341 gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt
    11401 tactatgaaa agagcggtca aggcttccct ctctttttac tgcatggcaa tgacggcagc
    11461 ggccgctttt tttcagaaca ggtccctgta ctcgaacgct actataccgt gtatctagta
    11521 gatagccgag gacatggtcg ttcgacaaac gaagcatcta tgttgaattt tcagttaatg
    11581 gccgaggatc tgaatacgat catgttgctg gaaaaaatcg atcaggcaga ttttcttgga
    11641 tttagtgatg gagcaaatct agcactggta tttgccagca gcttccctaa aaaagttcat
    11701 cgattgattt taaattctgg aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc
    11761 atcagtaata ttcattatgc atgggtatgg ttactctctt tatttcgccc aagtcttcgc
    11821 aaaaatcttt tagttatcaa acttttgctg catgacatcg ggcttacaga aaatgattta
    11881 aaaaaaatca actcccccac tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa
    11941 cattctttgt atatagctaa aactatacca aaagcttcct ttgtgcttgt gaaagaacaa
    12001 ggacatgaat tagctagaaa agatcctgaa cgcttcaatc gagaagttct tcaatttctt
    12061 tctgaaacat aactattagg aatagaaggt gatttatttg ttaaaaaata ccatacaatg
    12121 gctgaaaaac cacctaggac tgttcaaaac aatctttttg atttctgtca ttgtcattat
    12181 tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc cagcaattag gagaaacgtt
    12241 tgccactata cctgtatgga aaagcggact aatgctattg attggtctta tctctgtgtt
    12301 acctatggtt ggttatgata ttattttgaa tcggatgctt gaacaaaagc agaatcctcg
    12361 ttacctgttt gagacaagct ggctgatcaa tacgatcaac aatattgccg gtttcggcgg
    12421 gtttgttagt gtggggcttc gctctgaact atatggacaa aagaaagaga gcagaaacgt
    12481 gatccaagca ttatcgaaag ttttcttgtt tttgatggct ggactttcta tttacagtct
    12541 gctttctttt ttacttgtag tcttcacgcc agttgctcct tttttgaagc aatattggat
    12601 atggttgatc ggcggcggtc tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa
    12661 gaaaggattc cttggtggta cctcacttaa aactcaagga cagctattgc tagtttcatt
    12721 gttggaatgg accggtgtcc tgtttagttt cctttctgtt ggttatttaa tggaaatccg
    12781 gattgatctg tggcaaacaa ttccattatt cattgctgct tcagtgatag gaatcgtgtc
    12841 tatgattcca ggagaaattg ggagtttcga tgtaatgatg attatcgggt tgtccgctat
    12901 tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt tctactacat
    12961 cattccgttt ttgattggaa tcgtcttttt cttcaaaaat atcggttcca cttttgatca
    13021 gcgttattct ggtatcccta aacaattagc aacagaaatt gcgcataaaa tcgtggttgt
    13081 cttattatac ttttctggaa tcatgctagt actttctgcc actatcccgc aagcttttac
    13141 agagtttcgc tggcttcata gtttgaaccc attgaaattt cattttatta tccaatttcc
    13201 tagtatacta ttgggatttc ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa
    13261 acgagcatat ttgcctacaa tcttcttgat tgttctagct ttactttatg ttttattaag
    13321 tgattttagt ttcacaccgg tggttttctt gtctattctg cttctgatca ttcttgcttc
    13381 taaaaacgaa ttatttcggg aacaattgat ctattcatgg gagtggcgta cgatcgatgg
    13441 aatcattatt ggtgcactgt ccttgcttta catcgttatt ggggtataca atctgccgga
    13501 tttccctcat cggcgtcacc attttatttc ctttttctta tttccttctg aaaagatttg
    13561 gttttcggga ttgttggcca tcatcgctgt tagtttcatg atcgttctat ttgttcattt
    13621 ccttcaagga gaaaagaaac agattggcga agcctttaat gaagaaaagg ccctgaagat
    13681 ccttacaact tatggaggaa actccgatag tcaattgatc ttcttaaaag ataagcggat
    13741 gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc caattcgctt gcttcaataa
    13801 taaatgtatt gtaatgggtg atccttctgg taagaaagaa gattttccag aagcaatcga
    13861 agcttttatc gaagaaacag atcgactatg ctatttacca gttttctacg aaacaagcga
    13921 agagattgtt atgattttgc atgaattcgg ctacgatttc attaagatgg gggaagaagc
    13981 ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt
    14041 cttgaatcgg atcgaacgag aaggatttac atttgatgtc ttacaaccac cattttctac
    14101 tgagcaaatg agtactttca aaaatatctc tgataattgg ttgggttcac gaaaggaaaa
    14161 agggttttct ttaggctttt tctctgaaga ttatctacaa agagcgccta ttgcagttgt
    14221 caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa
    14281 taaagtagga acgattgatc tgatgcgata tgattctaaa aaagcacctt ctggcagtat
    14341 ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc aatggttcaa
    14401 tttagggatg gcccctcttt caaatgtcgg aacttccaga aagagttttt tacaggaaag
    14461 gattgcatac ttggtttacg agtttggttc tcatttttat tctttccatg gattaagaga
    14521 atataaaagc aagtacgcta caaactgggt ctcacgttat actctatatt ctagagatag
    14581 ttggattgca tatgttatga ttgctttgct gattattgat aatgcgccag ttgagcagac
    14641 acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa aactgcatac
    14701 cgatagatga acactctcat gatgaataaa agagactgaa gtaaaacgaa agttcatgct
    14761 tctacaatct cttcttatac aattgaagaa caaagagcgg tccaaacaga aatcctgttt
    14821 ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg cgtttcttat
    14881 gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg aattgagaat
    14941 gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt cacgttgtca
    15001 ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac atcagttgtg
    15061 cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc accttcttct
    15121 tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga acctggttta
    15181 gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa cacgcctacg
    15241 ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc tgttacaact
    15301 gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac acgaacagca
    15361 ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa gacgtcttcc
    15421 actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata ttcatctact
    15481 gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa tgcttttaat
    15541 gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct caataactcg
    15601 cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc aactttgttc
    15661 aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt
    15721 tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat ttgtgctgcc
    15781 cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg
    15841 cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg ttcacgttct
    15901 tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt tttgcctaat
    15961 actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc aatcgtacca
    16021 atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta caaacctcct
    16081 aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt ctttttttga
    16141 ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa gcttattata
    16201 cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg aaaaaagtac
    16261 cttttataaa taaaaaaatg tgttttttga gaaaaacttc aagatagtcc ttaactttct
    16321 tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc caacaacttt
    16381 caatagacga aatcttctat ttcgttaata ataaggaaag aggtgaagta catgaaaaaa
    16441 ttaattggcg tacttgtcgt tttaggcata tttctcggcg gaagttttgc agcttatcat
    16501 tatttttacg gcggcgaagc ttattataca aaaatcacga cgaatggcga aatgtcagct
    16561 acccaagcag ataacggaga gaaattcacg acttatacct acaagcaagc ggcttataat
    16621 aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc attaaagatg
    16681 aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg ggaagaaata
    16741 aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg ataattagta aatggtctta
    16801 aaattttatt agataaaa
//
LOCUS       DACEDO010000062        16589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEDO010000062 DACEDO010000000
VERSION     DACEDO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.062"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(81..788)
                     /locus_tag="IWO52_002135"
     CDS             complement(81..788)
                     /locus_tag="IWO52_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0356386.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(867..1205)
                     /locus_tag="IWO52_002136"
     CDS             complement(867..1205)
                     /locus_tag="IWO52_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0356387.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1316..2278)
                     /locus_tag="IWO52_002137"
     CDS             complement(1316..2278)
                     /locus_tag="IWO52_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0356388.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2293..3624)
                     /locus_tag="IWO52_002138"
     CDS             complement(2293..3624)
                     /locus_tag="IWO52_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0356389.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3632..4312)
                     /locus_tag="IWO52_002139"
     CDS             complement(3632..4312)
                     /locus_tag="IWO52_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0356390.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4531..5010)
                     /locus_tag="IWO52_002140"
     CDS             complement(4531..5010)
                     /locus_tag="IWO52_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0356391.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5137..6816)
                     /locus_tag="IWO52_002141"
     CDS             complement(5137..6816)
                     /locus_tag="IWO52_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0356392.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7364..8866
                     /locus_tag="IWO52_002142"
     CDS             7364..8866
                     /locus_tag="IWO52_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0356393.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8879..9541
                     /locus_tag="IWO52_002143"
     CDS             8879..9541
                     /locus_tag="IWO52_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356394.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9642..9845)
                     /locus_tag="IWO52_002144"
     CDS             complement(9642..9845)
                     /locus_tag="IWO52_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0356395.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9956..11140)
                     /gene="iadA"
                     /locus_tag="IWO52_002145"
     CDS             complement(9956..11140)
                     /gene="iadA"
                     /locus_tag="IWO52_002145"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0356396.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11297..13036)
                     /gene="yfcC"
                     /locus_tag="IWO52_002146"
     CDS             complement(11297..13036)
                     /gene="yfcC"
                     /locus_tag="IWO52_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0356397.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13809..14423)
                     /locus_tag="IWO52_002147"
     CDS             complement(13809..14423)
                     /locus_tag="IWO52_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0356398.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15029..15406)
                     /locus_tag="IWO52_002148"
     CDS             complement(15029..15406)
                     /locus_tag="IWO52_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356399.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15706..16587)
                     /gene="galU"
                     /locus_tag="IWO52_002149"
     CDS             complement(15706..16587)
                     /gene="galU"
                     /locus_tag="IWO52_002149"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0356400.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4809 a         3573 c         2831 g         5376 t
ORIGIN      
        1 tattccaaat aaatggtatt caatcagcta actctcggta ttagctcaga tcaaagcgct
       61 gatttcacaa cttctttatt ttaatctctt cggttgccgc cgaacaataa gatcaagcgc
      121 aacagctgca ggaatgtcga aagcgctgct gcaacataag tcagtgctgc tgcaaataat
      181 acgtgtcttg ccatcggtac ttcttcttcc gtcagtagtc cgccttcact caaaatttgg
      241 attgctcgcc gagacgcgtt aaattcgact ggtaatgtca ccaattggaa aagtaatgct
      301 aaagaaaaag cgaaaatccc gatattgatt aacgtagagt tccagctaag caatacacct
      361 accataatca aggggaacga aatcatcgaa ccaaagttag ctgctggaac aagtgctgca
      421 cgaatcttca atgggacata acccgaagca tcttgtaccg catgtccgca ttcatgagct
      481 gctacaccta tcgcagcaac tgaagtagac tgtgcagttg cttgagacaa actcaacatc
      541 ttattacctg aattgtagtt atctgtgaga tctccggcaa tttgttgaac accgacatta
      601 tttatctgtt gactttgtaa tatatattgt gctgcttgtg taccagtcac attgtttttg
      661 cttctcactt gatcgtattt tcggaaagta ctattgacgt acgctgacgc aattccggaa
      721 ataactaatc caagaatgac caaaagaaat gtcggatcta aaaaaccata cattccataa
      781 ccatacattt tcgtttcact ccttttcgtt tactttattt tatcattagt agtaggaaaa
      841 gaatgtgaaa agaccttgaa tattctttaa ttctttttca caaattccga tttcagtttc
      901 atcggtccaa atccttctac cttgcaatcg atattatgat cgccttctac taaacggatg
      961 ttcttgactt ttgttccttg tttaatggca cctgacgcac cttttacttt caagtcttta
     1021 atcactgtca cgctatcgcc ctctaacaga cggtttccat ttgcatctaa taccactaat
     1081 ccgtcttcta tgacagtttc ttgttcttct ttgttccatt cgttcccgca ttctgggcaa
     1141 atcaacaagc ctcgatcttc atatgtgtac tctgagccac attctgggca atttggtaat
     1201 gtcatcttct ttctccttta aaatctttag tgagcactat tgtaacggaa aaagaagatc
     1261 ttgaaaagat acatttgtga tttttactac aaaaggcacg ttttcgaacg aagtatcatt
     1321 ctgtttttct gctcttatac gattggcata tatcttggat ctctttatag atttcctcta
     1381 cttctgctaa atcttttgca ttcgcaccac ttgctaatgg atggcctcct ccatgatggc
     1441 gtttagccaa ttcattgatg atcggcccct tagaacgtaa acgtacgcgg taataccctg
     1501 aaggctgctg aacaaaaatc ccccaagcca gcacttcgtc aatcactcca ggaagtgaaa
     1561 cgatcgaagc tgtctctgaa tcatcgattc catatttatc aagaatttct tgcggaagga
     1621 ttattcttcc tgcaccattt tcatccacct ggatattttg ataaagataa ccggaaagtt
     1681 tagcgacttt catcggcatt tgttcaatct ctctgttcaa agcacttgca tcaaatggat
     1741 aatttctcaa tatcgaagca atctctaatg tacgggacgt cgtggaggga tacaaaaagc
     1801 gccctgtatc tccgacgatt cctgcataaa gtaaccgtgc agccttaacg ttcatcttta
     1861 aagattcagg aaacatcaaa gcaaattctg caatcatctc actacagcta cttgcttttg
     1921 tatttaccca cacaagatct ccataagggt catcattggg atgatggtcg attttgatca
     1981 gtttttctgc caactgatat ctttgatcac tgattctggg tgaattagct gtatcagtca
     2041 cgatgactag cgctccttgg tagaagacat cttcaacttc ttgcatttct gctaaaaagc
     2101 gaagcccttc aatcgtttca cctacttgac gtatttcttt ttctgggaaa ctttcccgta
     2161 atagttcggc taatcctact tgtgatccta atgcatcagg atcagggcgc aaatgcctgt
     2221 ggataatgat tttatcgtat ttttttattg tatctactat ttcttgctgt actgtcattt
     2281 cactcctgct ttctaagaac gttccattac ttgacaaaca acgattgctt tggaaacgat
     2341 ggtgttttca ataaacactt cgatatccag tttagcggaa cgtctgccga tttccaatac
     2401 acgtggacga atgtctaatt cgctttccaa ttggatcagc cgcaaataat gtaggttcac
     2461 ttgttcaatc aagatatttc gtttttggtt catcaccatt gttttttgtg cgacatcaga
     2521 aatgatttcg ctaagtacgc caaaagaaat ggttccaacg ccattgacca tttgtggtgc
     2581 tactacgaat ttgaacttag ggttttccaa acgatttcct tcacggtcag tatcaatcgt
     2641 atggatattt ccagatatct gatccgaaat cgtatctgca atctgcggtt gtcgctggac
     2701 aagctgcatc gctttcatta catcttgccg ggtaatcaag ccttccaatg tcaagtcatc
     2761 ggaaacgacc ggcatgactt ctagcccatc ccagatcatt tgatggctaa cagaggcaat
     2821 actcattgtt ttctttacac tgattggttc tttcgtcatc acccgatcga tgatctggtt
     2881 tggcgatttc cctaaaacat cttttgccgt aacaatcccc atcaaacgat gatggcggtt
     2941 gacgactgga aaacgcgaat gctgcgtttc ttctgacaaa cgctgataat cagaaatcgt
     3001 atctgtctgc tgcaaataat gagtcttttc agctggcata tagatatcac tgacaagcat
     3061 gatatctttt ttgatcaatt ggtcgcttaa cgctcggttg atcatggttg cgactgtaaa
     3121 tgtatcataa gaggtgcgta agatcggcat ttcaagatca tcagctagct ttgcaatctc
     3181 agcagtcgta ttgaatcctc ctgtgatcaa tactgcggcg ccatcttcca atgctaattt
     3241 ttggacttcc gcccggttgc cgacgatcat tagtgatcct ggcgtaatat agcgttccat
     3301 ggcatgttta gtcatcgcgc cgatgacgaa tttattcaag accttgtcta atcctgccgc
     3361 cccgcctaaa acgtcccctt caatgatcct gacgacctcg ccaaatgtca aacgttcaat
     3421 atgttttttt aatttgcgtt cgatacggat ggtccctact cgttgaattg ttgaaactag
     3481 tccgacattt tctgcatctt tgattgcacg gtacgctgtt ccttcactga cattcaattc
     3541 ttttgcgatg cttcttacag aaattcgatt gccaacaggt aaactttcaa tatattctaa
     3601 aatcaaatca tgttttgtcg ccattttctg cctacacttc cagcgtttcc gagacgctca
     3661 ttatttttcc ttcactatct tccagtaact ctacaaaaga ttcgggatct tgttcaatga
     3721 cagggaaagt attgtaatgg attggaacta ctttcttggc acgaattgtt tgcgcagcct
     3781 ttgctgcatc ttctggcccc atcgtatagt tatcgccaat cggcaagaaa gcgacatcaa
     3841 tatcgaattc ttctgcaagc aatcctagat cactgaaata agcagtatcc cctgcatgat
     3901 aaatcgtttt tccttctgct tgaataataa ttccgctagg ctcgcccata tactgaacga
     3961 taccgtctac ttcatagctt gaactatgct gtgcatgtac gaattttacc acgccgaatg
     4021 gaaacgcatg ttttccaccg atattcatac cgaatgaatc tactcctctg ctgttcgcat
     4081 aatcagcaat ctcaacaata ctgataattg tggcttgatt ttcttttgca ataggaatca
     4141 tatctcctat gtggtcacta tgaccatgag taatcagcaa ccaatctgtc tttacttctt
     4201 ctgcagttaa gtctgtcaat ggatttcctg taataaacgg gtcaatcatc acgcgttggc
     4261 cattttccat ttcaattgtt aaacaggaat gtccatgata cgtaattttc atattttaca
     4321 cctcgcgttt tctctgtatc ttttttatta aaaacgattt catcacttgc acatcaatga
     4381 aaaaatatag ctgctaaata tattttcatt ggattgatag tacagtatgt acaaagcaat
     4441 tgtaacagat aatacaggat gcaccaagta ccaagcagat ataatctcaa gtttgaggtc
     4501 tctgctctgc ttagcttctc ttctcgtccc ttaactaatt aaatcagcgt aaataggatg
     4561 gacgatcttt tctaaatcgg ctacttttat ccgaaggctt ctcccgattt caccgcttga
     4621 cacaattact tcttcttttt cttttaaaga actgtctgct aaaaaacaat aatcaggatg
     4681 tgcgagtttt attccaatag gggtgtttgc tccgtgggga tatcctgtat gcattaatac
     4741 aaaatccatt ccaggaaaac caactttacg atctttcgtc acttgtctcg tttttttgta
     4801 atctaagtga tcgttcaaag gaatcaatgc gatgatcacc ccagttttgt ttcctttcac
     4861 tacgagagtt ttacatatca aagctggatc aatccctctt ttttgtagtt cttctgtgaa
     4921 agattcttct accacatgac taaaatcaag tggttgatag ctgatctttt gttcttctaa
     4981 ataacgttcg acttttgttt gactcatcat tgattcactc cctttattct atcgtttatt
     5041 ataaagcgag ggatgctgta ttcaaaaatt atttgttttt cgcaaaaaaa gagcaagacg
     5101 agcactctat agagaggttc atcttactct ttttgtttat tttttatttc gacgttgagt
     5161 agcttccata atgactggca agatcactgg gcggcgtttt gtttgttcaa ataagtaacg
     5221 gcctaattga tctcggatgt cttgctttaa tttgctccac tcaaaatcat tgctttctaa
     5281 atgtttctca acgattgttt caatcatatt gctgctttct ttcatcagat cacggctagc
     5341 ttttacgtaa acgaatcccc gtgaagtaat ttgaggtttt gaaatgattt tcttttctcg
     5401 ccgactgata gtgacaaccg ctacgaaaat cccgtcttct gacaagattt ttcgatcacg
     5461 taaaacaatg tttccgatat cacctatgcc taatccatca atcattacat tttctgcact
     5521 tgtagttcct gcaacactca tacgcttgtt cttatattcc agcacgtctc cgcgtccagt
     5581 aatatagata tttttgtatg gcatccctac tgcatgagcc aaatctgcat gagcagctaa
     5641 ttcacgatac tctccttgaa ctggcacaaa atatgttggt ttcatcaaat tgatcattaa
     5701 ttgcaagtca ttaggattgg catggccaga aacgcgcata ttctcagaga tcaacttcac
     5761 tgttccacca gctcgataaa tgatatcttc cgttttcgcc acgatggttt ccatcgcgat
     5821 gcttggcgta gtagtgatat aaaccaaatc tccttcttca atcttgacat tacggtgtgt
     5881 accgtttgcc attttttgca gagacttgat gggttcaccc attcggccag tttctaaaat
     5941 gatcagttct tctggatcat agtttttcat gtctttcaac gtaacaagca gatcttcact
     6001 tggtaattga agtttttcta aacgcatcgc tgtgcgaata attcgttcga aatcttgacc
     6061 agtcaagaca acttttcgtt gtgctttttc tgctgcatcc aatacttgtt gcactcgttg
     6121 caaattactt gctacacaag cgacgatgat acgcccttcc caatatttga tcgtatcaaa
     6181 tacttcatct gctatttggg cttcagaagc tacaggctga ggattttctg catttgccga
     6241 ttcacttagt aaggctaaga ctccttcttt gccgatttcc gccaatcgtg cataatctgt
     6301 ttgatacatt gggatagccg tttggtcaaa tttaaaatct cccgtataaa cgatactacc
     6361 ttcttttgtt ttcagattga tccccactga atctggtatc gtatgagtgg tacggaaaaa
     6421 gctcactact gtttcaccaa agtctatttc tgtatgctca tctaccacat ggaaatcctt
     6481 aaatcctttg gctgcttcat tacgactgac attcaatttt gctaactcga tcgtcaactc
     6541 tgtcccgaat actggtacag aaatttttga caacaagtaa ggaagtgccc cgatcgcatc
     6601 tgcatgtcca tgtgttaaaa agacaccagc tacacgatca atgttttctt ctaaatacgt
     6661 aaaatcaggg attacgacgt cgatccccaa caactcattt tcaggatatt ttagcccaca
     6721 atctaaaata aaaatatcct cgcccacttc tgcgacatac atgttttttc cattttcacg
     6781 aacgccgcct aatgggataa ttttaatggt actcacaaac ttcacctctt cttatgacat
     6841 ccaagtttaa agatgtcgat tttcttcttt atctaaatat atgagccatt ccaatgaagc
     6901 atatgcaaaa tcactatttc tcaacttatg gagaatcagc aggaaaatta ccagcacatc
     6961 cgcgttctga cagttggatg ggcattttat tttggatata cttcattaac aaaatggatc
     7021 ttttgcgaaa cgctatagcc agaatactta tataagtatt cagctgttcc ccattattat
     7081 acaacaaata aactaaatcg ctagtatcag caattgttag aacaactatg cacgtaaaaa
     7141 tccctctgta gcatctagga tttcttttgc tattcctctt tcatttcttc ttcgtcggtt
     7201 tgaaacatac ttttttcatt ttaccacatt ttttgcaata actatagtga ctttttatat
     7261 atacagaacc tcatgttgat ggtgtgcaaa cagcataaga tttaagctag ctttaagata
     7321 ggttgtctat tatcaaatgc atctactagg agggatgcaa aatatgaatt ttatgaaacg
     7381 tgcatggaaa agtacaaaag taaaatgggg acgttcattg ttattgtttg ccgtgttcac
     7441 agccattctt atttttgtgc tagctggcct cacgattcga agcgctgcag aagaagcagc
     7501 gaatcaagca caaaaagaag ttggagcaac agtgacttta agtgccaatc gagaagcttc
     7561 atttaaaaaa caggaacaaa cttcttcaag cggttctacc actgaagaac gtccagatcc
     7621 aggcagtttt tctcttacac cagtttcctt aacggacgcg gaaaaaatag ctgaattaga
     7681 taatgtaaag agttattcct ttgaaagttc cgcctcagct atggcaaaag atggaataac
     7741 tgccatcact agttctgatt caagtgaaga cagttcagaa actactgatg caggcggtga
     7801 acctgctgga ggaatgggcg ggaatccgca aatgactcaa gcagactttc aaatcagtgg
     7861 tgtgtcagca actgcacaaa atagtaattt ttctgatgga acagcaaaaa tcacggatgg
     7921 tgaaggaata gatgacagcg acaaaggcac aaacaatgta gtcattgatt ctactctagc
     7981 agaaacaaat gacctggcag tcggagatac tttcacaatc acgagtactg aagatgaaga
     8041 taccacttat gaaatgacga tcaaaggaat ttatgaatcc agcgaaacaa gcggcagtat
     8101 gggtatgaaa ttcaacttca tgaatccgtc gaatacactt tacacttctt acacttttgc
     8161 aaatgaacta aatgggacat ctgacgataa cacgatagat tctgctattt acactctatc
     8221 tgatccagat aaaatggatg agtttctcaa agaagctgag aagctgatcg acacagatac
     8281 atttagctta caatcaaatg attccatgta tcagtcaatg ttggaacctt taaacaacgt
     8341 ggcaagtttt tctaaaaatg ttgttttact agtgactgta gccggtatta tcatacttac
     8401 tctgatcatt atgattacca ttcgggagcg tcggcatgag ttaggcgtct tgctatcttt
     8461 aggagaatcc agaagtaaaa tcattctcca attgtttacc gaagtggcta tttgtatgat
     8521 tcttgcatta ggagttgctt cgcttagcgg aaacgtcgtc gcgaatgccg tgggacagca
     8581 gctacttgac caacagacag aaaccacgac acaaaaccag caagctcctg gcggtggaca
     8641 aatgccaggt aacagacaag aacaaggcgg tcaacgcgat ggtcaacaag gaaccacgaa
     8701 taatccattt gctgtttctg atcaagtcag cgaattggaa atcaatgtca aacctgcaca
     8761 attgggacta ttagccggag tagcatttgg aatcagtctg ttttctgtct ttttagcttc
     8821 tatagggata ttaagattaa acccaagaaa aatcctatta aactaaagga ggaatttgat
     8881 gttacggaca gaacaattgg gattttggta tcaagatccg caagatgcgc tttataaaga
     8941 tgtagaactg aatttcgaag ctggaaaaat gtacgctatt ttaggagcaa gtggttctgg
     9001 aaaaacgact tttctttcgc ttatttctgg actagatgca ccaaaagaag gaaaggtata
     9061 ctatcaagat cgttcactga acaaaatcgg gttaagaagt tatcgtaaaa atgatgtctc
     9121 gattattttc caagcttaca acttgcttcc ttatttatcg gctctagaca acgtcatagc
     9181 agcaatggaa atcactaaga ccaagcaaga gaattatcga caagttgctt tagatcgact
     9241 ggctcgtgta ggaattgatg aaatattagc aaaaaaacag gtgaccaaac tttcgggagg
     9301 tcagcagcag cgagtcgcta tcgttcgtgc tatctgctgt gaacatcaac taatcgtagc
     9361 ggatgagcct actggaaatc tagatgaaac aacttcacaa gatatcgtca aacttttcca
     9421 agaaatcgcc catgaacaaa atcgctgtat tatcatcgtg actcatgaga ttgaagtagc
     9481 aaaaagctgt gatgaagtct ttgaactaaa aaataaaaaa tttcaaaaaa tcgaagttta
     9541 aaaaacagga taatgatcaa ataaaaaatc cgaaccatat ctgtctcgca gatatggttc
     9601 ggatttttta tttgcttgat tcaattagta ataagtggtt tttatttttt ttcgtctttt
     9661 cgagaaaact tgtctttgac ttcgtcgcct aaatttcgag ctttttcttt gatgtcagca
     9721 cctagtgatt gtgctttccc ttctgtttct ttgctcttgt cgtctgttgc tttgccgtat
     9781 gcttcttttc cttcaccgac tactttatct ttcatatctt tagaacgtcc ttttaaatct
     9841 gccattgtgc ttcctcctct tagtttgttt attgatctac cttcagggta acatcaacaa
     9901 cagatacaga caaacagaac gctcgttttc taaaaaagat agttagagct gttgattaaa
     9961 aacttttttc gaaagtcccc catactttga cttctttttc ttggaccatt atttctcctt
    10021 tagcgataac tgtatcaata tcaagcgttt ctttatccaa gatacacaga tctgcatcag
    10081 caccaatttc gatttttcct tttttatcca atttcaatat acgtgctgga ttactggtta
    10141 tagctcgtaa ggcgatttcc aaagggatac tttctttttg cacagcttct ttgatcccta
    10201 ctagcaatgc cttggcactt cctactccta accctaggaa atgattgttt tcatcaaaat
    10261 aaggcaagct tccttgtcca tctgaggtca tcgtgaaatt atctaaagaa acatcttcct
    10321 cgatcaatcg tttcagtgct ttgctgaagc gaacttcacc atctgtcttc tcccaaaaat
    10381 caggatcttc gctagcagtg aagtcaatcg tgccaccacg tttggcaaat gccacgcaag
    10441 cttcaaataa ttctggtgtg cgatttgcat gagtaggcaa aaattgtgtg atcggaattt
    10501 ctgtttcttc tacgattcgt gtcaatagct ctagctttct ttttcctccg cctaagtgga
    10561 cattgatgat cccagctttt ccggataaca tgcctcctac acgagcatct gctactgcac
    10621 gagcgaactc ttcaaaactt ggctgagaag aacggtgatc ggatactgcg atttctccga
    10681 ttccgataat tttatcaatt gtcaaaaagt ctttgatgat agaatctgtt actgttttta
    10741 ccggcaatcg gtacgaacct tcgtatacat aagtggagat tccttctgct tccagacctt
    10801 tcgctttgct gatcaacgca gtcatatctc taccttctcc gtctgttccc aaacaaccga
    10861 caactgtcgt tacaccagct gtagtcaatt ggctcaatgt gacttctggc gttcgatttt
    10921 gataaccgcc ttctcctcct ccacctaaaa tatggaaatg acagtcaatg aaacccgggg
    10981 ttagtacttt tccctcacct gaaagttctt ctacttccac tccttcgtag ccgccttcta
    11041 gctgttcttc tacagccaaa atcttcccac cggcaatcag tacatccatt tttcctaagt
    11101 aatctggtgc aaacacttca atttgtcgaa tgattttcat acatttttcc tctttcttta
    11161 agtataactg atgataggat cttaaaaaat aatccaaacg ataggatctt tactcctttg
    11221 attttaccgt aaaatgacgg ttcttggagt ccttatctac tgtttggatt acttttttat
    11281 ttacaggtct ccagtactac tgcagaccta ttgcaatggc aatcatgatg aaaatagttc
    11341 ctaaaacaaa caggaatcct tgcatcttga tttggaatct tgcccatttt gcccattcga
    11401 tccgtgcaac acctagcaca ccaatcaagc tggctgatac gggtgtaaaa gcgtccataa
    11461 aacctgaacc aagctgataa gcaagaactg ctacttgtct ggatacacct actaaatctg
    11521 ccagtggagc catgattggc atagtcaaag ctgcttgtcc tgaatttgat gtaacaacca
    11581 agttgaacaa actttggaag atgtacatcg cccaagcacc aatgacagct ggaacaccgg
    11641 tcaacgccgt cccgatccca tgcaaaatcg tattcaaagc tgaaggaaca gatgcatcag
    11701 atcctcccaa gacaagaaga atccctttgg ccatacctac tacgattgct gtaccagcta
    11761 aatcagctgc accagattgg aaagaagaag cgatgtcatt gactttcatt ccatctaatt
    11821 tgaaaagaac agcaatgatt ccagcaacta atcccatcac aaagaattga gaagcgattt
    11881 ctggaatgta atagcctttt tgagtcacgc cccaaacgat ccagacaagg acaactaaca
    11941 tctctaataa gatcaattta tgcccaagca tgaatttttt attttcatca gatgtctttt
    12001 tgatatgaga acggaaataa tcatctgatt tgtaagtcaa cgaactttct ggttttttac
    12061 ggattttttc tgcatatatc atcatataac cagcggctaa tgcagtaaca actacccaca
    12121 taatcaatcg gaatgtcgca ccagacaaca cggggattcc agcaatccct tgagccaccg
    12181 ctacgctaaa cgggctcatc caagaggtcg catttccgac ttgagatgcg acataagtca
    12241 cggttactgc tacgatcgag tcatatccta acgcaatgac aaatggaacc atcaccattg
    12301 agaatggaat tacctcttct gccatcccga atgtagcgcc accgaatgaa aaagcaaaga
    12361 ataatagcgg taaagctaaa cgctccagtc ccttagtttt actgataaac gcatagatac
    12421 ctgcgtcgat cgctccggta cgcataataa taccaaaagc acctccaaca acaaggatca
    12481 atgcagcgat ccctacagca gatccatact tatctccaga tactagtcct tcaaaaacaa
    12541 aatttaagaa accaaagccg ccaaaatcat ctgttcccca tattttggct gttttatgca
    12601 attttttaga agtatcatag atattttcgc cgtactgatc gtaaagcaca tcatctgtca
    12661 aggagatttc atctagtttt tcctgcgtaa gatttttttc tccgtcagca atgacttttt
    12721 ccaatccttt ttccggaaca tccagttttt cacgttctgc tggatgatct tgcagttctt
    12781 ctaattgatc aaacacatac gacttatcca gctcgtaagc ataacggaag gaatcttgac
    12841 gtaaaacagt acgagtactt gtttcgccat ttgcatcttt gtattcaata tcttgcgtgc
    12901 tgaactttcc tgctggaacg acaaaagtca gtatccatgc aaagatgact acaccaaaaa
    12961 taatgacata cgtatggggt gtcgataatt tagtgaagtc gcgtttcggt tttcctgaag
    13021 aatttgtttt cttcaaattt atttcttcct ttcttttttc agtaattttg atgaataaaa
    13081 aatggataat caaatgaatt atacagtaaa aatgacattt ccacatgaat aatttattag
    13141 aaaatcactt ttttctattt tgtttttttt ctcctatgta tgattatttt tttctctttc
    13201 catttatatt aagtatttta tttctctgtt tttccttaaa attctttttt tatttttaga
    13261 ttagagaaaa tctcgtcctg tttttttcat tttacaatca ttgaaaaatt gaatttcatt
    13321 gcaaaaaaaa taataaacac ttataaatat ccattatcta tccttcaatt tttgtgtatt
    13381 ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg taaacttcat
    13441 tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg gaagactctt
    13501 tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt
    13561 ccactacttt ttatttttct gacactacat attcttataa ctcatttctc ataactgtga
    13621 aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat
    13681 cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct
    13741 ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat
    13801 gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact
    13861 tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt
    13921 ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat
    13981 tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca
    14041 gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc
    14101 gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga
    14161 tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca
    14221 atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt
    14281 agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa
    14341 gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa
    14401 tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga catgaataga
    14461 aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg
    14521 gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata
    14581 gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct
    14641 agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca
    14701 gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc
    14761 ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca
    14821 agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa cataaaaaaa
    14881 ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga
    14941 aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt
    15001 atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt
    15061 cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact
    15121 ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata
    15181 cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg
    15241 ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa
    15301 atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact
    15361 tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc
    15421 tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt
    15481 catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca
    15541 taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa
    15601 ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt
    15661 taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct
    15721 tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg
    15781 aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt
    15841 tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt
    15901 tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg
    15961 agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt
    16021 ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat
    16081 tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt
    16141 ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc
    16201 ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc
    16261 ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc
    16321 aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt
    16381 tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc
    16441 gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg
    16501 aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc
    16561 aggaatgact gcttttttga ccttcactt
//
LOCUS       DACEDO010000063        16365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEDO010000063 DACEDO010000000
VERSION     DACEDO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.063"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..723)
                     /locus_tag="IWO52_002150"
     CDS             complement(85..723)
                     /locus_tag="IWO52_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0356401.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(846..1478)
                     /locus_tag="IWO52_002151"
     CDS             complement(846..1478)
                     /locus_tag="IWO52_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0356402.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1829..2128
                     /locus_tag="IWO52_002152"
     CDS             1829..2128
                     /locus_tag="IWO52_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0356403.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            2623..4788
                     /gene="acm"
                     /locus_tag="IWO52_002153"
     CDS             2623..4788
                     /gene="acm"
                     /locus_tag="IWO52_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0356404.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(5060..5545)
                     /locus_tag="IWO52_002154"
     CDS             complement(5060..5545)
                     /locus_tag="IWO52_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0356405.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            6025..6666
                     /locus_tag="IWO52_002155"
     CDS             6025..6666
                     /locus_tag="IWO52_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0356406.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            6685..7320
                     /locus_tag="IWO52_002156"
     CDS             6685..7320
                     /locus_tag="IWO52_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356407.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            7338..8153
                     /locus_tag="IWO52_002157"
     CDS             7338..8153
                     /locus_tag="IWO52_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0356408.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            8472..9908
                     /locus_tag="IWO52_002158"
     CDS             8472..9908
                     /locus_tag="IWO52_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0356409.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            10097..12544
                     /locus_tag="IWO52_002159"
     CDS             10097..12544
                     /locus_tag="IWO52_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0356410.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            12774..13841
                     /gene="rlmN"
                     /locus_tag="IWO52_002160"
     CDS             12774..13841
                     /gene="rlmN"
                     /locus_tag="IWO52_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0356411.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(14034..15137)
                     /locus_tag="IWO52_002161"
     CDS             complement(14034..15137)
                     /locus_tag="IWO52_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0356412.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            15690..16238
                     /gene="aac(6')"
                     /locus_tag="IWO52_002162"
     CDS             15690..16238
                     /gene="aac(6')"
                     /locus_tag="IWO52_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0356413.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         5673 a         2647 c         3328 g         4717 t
ORIGIN      
        1 atccattgat acatctgtct ttttgtttaa aggaaataaa gtatgctttc ttagcaatag
       61 agcatgtatt atttctataa aaatttagga gaataaaacg actagcaata tagctgtgaa
      121 atcaaatatc acatgaactg cccaacttgt aatgatcgag ttgcttttaa tggcagcttg
      181 gttgaataac aatcttgcca ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat
      241 atttccatta tcataagtac tgtaatggat aaatccaaag atgattgctg acaagaaact
      301 agcgacagga atgctgaact tactagaaaa aatagcgaga aaatacactg agaacaattc
      361 ttctccaagt atcatgatag gcaaaataat aaataataac gggctgaaag cctcatttat
      421 tggattgcct gttaaacccc atttcaaaat ttgttgcaaa aataccgaga ctgcggcact
      481 gattactaca ttgataaaaa aatatttggc tatatttttg acaggagcga caggtttcga
      541 gaacattttt ttcgtatctt ttttaccaaa aacaagtgta aatgctataa atccagtgat
      601 agttaaagaa aaaatgctaa taatggatgg cgcaaatagc aaccctaaca aaccgagtac
      661 aacgacaata gtgcccaata tccctttttt aagttctatt ttttttccta aaatgttatt
      721 catgataata actcctacta ttcagttttt gaagatagag tcagtataca tgaagagtta
      781 aaataggcaa tgttttttta tggatatttg tattgctaaa aatttatctt atatattcat
      841 tttgattata ctacttttat ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga
      901 ttaagcgtac aattttttag atgttccgag cgtacttcaa tcatttcaaa ggagttgcta
      961 cttaaatcga tattttttaa atcagtatca aaaaagctag cattttctaa atcattagta
     1021 gaaaacatta ttttagtgag ggaacagtct acaaaatctg cgcttgataa gacagagtgc
     1081 tcaaaggtac tttcttttat gtttgaacca gataaattta catagtccat tttacaattc
     1141 tcgaataacg tgttcccaat ataactttcc atcaatttag tacctgttaa tttacaattg
     1201 acaaatttca cgcgatgcaa catgcttttt gtcaatatac agcctgaaaa atcacagttt
     1261 tcgaatatac agtcggtaat ctccattctt tgaaaatcat tggaagcaaa aaatgcatac
     1321 ttgaaaatag atttgtaaat aattggatgg cagattattt ggttttcgat gacctctttt
     1381 tgtgaaagga tattatacag ttcaaaatct tcgttttcat agatatgaaa aaaggatcct
     1441 ttattcaagc ttgcttcatt aactttagga aaaatcatat ttaattacct ccattttcat
     1501 aaagtcatat taacaaaagc taaaccaact tacattagca gaaaatgaga taggttttcg
     1561 cctttttcga taacgatagt gtcgctatct cttgaaacgt tcttagaatt gccctgagag
     1621 acacaaatta ggcgtaattc taataaaaat cttagaataa aaatttttac tgatttaatt
     1681 tagaatgttt ttaaaataaa aatcgttgcc ataacagtgt attgaaaggt tttaacaatc
     1741 cagtaatgat ttaaaaaaat ctagatgtag cgtatacttt gtttatagaa tgtagtggga
     1801 agaaatatat ttttgggagg aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt
     1861 actaaaatta tatgatctcg ttttaaatcc agagatcaaa gaaaaggaac gtatactttt
     1921 gatagacgca aagacaggct tagaaaaagg tcagtattat cctaaagtca taaataattt
     1981 ggaacgtagt ttacgtccgt tagcaatcag gggagaattg tctaaaccag tatcaccgtt
     2041 ttatatggag atatcaacaa taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc
     2101 ggcaccgatt acttttgggc atttataaaa acgagttatt catctcaatg aattaaaaag
     2161 tatagtcaat ctcataatgc aggctaggtt catttatttg aacagagtct gtattttttt
     2221 attctaaata aagaaacaga tgtttcttta tattgtattt tatcctttaa ttaacacaaa
     2281 aacagctagt ataaactaat aatgttaatt aaaagtgtat tttatgaaac tatcgttatt
     2341 tattaatgtt gcatttttgt aagttaaaaa ttggaaatat agattgaaaa aatgaaagca
     2401 ataagtaaaa taaaatgaat atatgcgaaa aaataattta cattaggaaa aaccttgtaa
     2461 aaacagtttt ttggcatatt tattttttat aaatatttgt aaaataacta tcaatttttt
     2521 agttaaaaga ttggaaatga gtgggggagg aattgaagaa aggatttttg agagatgata
     2581 tagtaggttt tatttttagt taaaataggg gagatagaaa aaatgaaaaa gtgtagaaat
     2641 attattttat caatgttatt tattattact aatataacat cactgattcc tgttcatgtc
     2701 tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt ggacccaacg
     2761 aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat
     2821 atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa
     2881 ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac tttaacggtc
     2941 agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa
     3001 ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga agaaaatacg
     3061 ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac aaagccagct
     3121 tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc tgaagatact
     3181 aagcacattc gatggttttt gaatattaac aataacggca cttatgttga acaaccagtt
     3241 aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt tgaaattaat
     3301 caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca acaattttta
     3361 caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat tgaaataact
     3421 ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac gatcatagaa
     3481 aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga atttaataaa
     3541 cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc ttctggtgga
     3601 gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga cacagagatt
     3661 ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat tcaaggacag
     3721 tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg cctgcaagta
     3781 ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga tccactaagt
     3841 tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag tttacctatt
     3901 tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg tggaaccact
     3961 ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg ggaaccagtt
     4021 cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg ggaagatatc
     4081 caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt agatcaaaac
     4141 ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt cgttacaaat
     4201 gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa tgagaaccaa
     4261 gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca accgatccaa
     4321 cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct gccaaaatac
     4381 agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga atacagtacg
     4441 acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa tatatctata
     4501 ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag agagaaaatt
     4561 agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc cagcgaacaa
     4621 agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa atcaaaaagc
     4681 attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg tttattgata
     4741 gtaactatct gtgctgggat attcttacaa aaatataaaa aagtttagtt agaatataat
     4801 attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa
     4861 gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat ttggctaact
     4921 atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt gtttgattgg
     4981 ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg aattgggaaa
     5041 taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct aagtaaacaa
     5101 gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata tttggatgaa
     5161 agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt tttttctctt
     5221 tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg aggtcaggaa
     5281 gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct agttgtttta
     5341 aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct tttcttgatt
     5401 tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt ttgactgacg
     5461 taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt agttcataag
     5521 cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt agtgtatcat
     5581 aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt aaaacaaatg
     5641 acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg gtggaaagag
     5701 gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat agtaggaaaa
     5761 tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc tggcttttcc
     5821 gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat cttcggatca
     5881 aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa
     5941 aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca agaggatttt
     6001 tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc gcaattatta
     6061 gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc attaccatta
     6121 gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta cttgcttcaa
     6181 acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt tattttttat
     6241 gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat tgcctttatc
     6301 ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc gattcctcaa
     6361 ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc
     6421 gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt tatcaacttg
     6481 gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa
     6541 atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat catctatctt
     6601 acattgactg gtattctaac aatcataatg aaacaagttg aaggaaaatt aaattattac
     6661 cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa agcatttggt
     6721 gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac attagctatc
     6781 gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg cttagaaagt
     6841 tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga gcttaaaatg
     6901 aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca tctgaatatt
     6961 tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga agaatacacc
     7021 aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa tatctatcct
     7081 tatcagcttt ctggcggaca aaaacagcgg gtagcgattg ccagagcatt ggctatgaat
     7141 ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt acgccagcaa
     7201 gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat tgtcacccat
     7261 gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc aattcattaa
     7321 gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc gcaggtcttc
     7381 ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg actatcaacg
     7441 aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga tttagagata
     7501 aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt gaacaatatg
     7561 ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg
     7621 gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc
     7681 aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa
     7741 tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg tcatctggtt
     7801 atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac caagatgctg
     7861 ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg gtcgatggtc
     7921 tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa ctagaagact
     7981 tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca cgcaagtcag
     8041 atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa acaggtgaat
     8101 tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat taacaaatag
     8161 gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg ttctcctgcc
     8221 aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa
     8281 aagccagttt ttcggtatta agccaaattt atgcaaaaat gtagaaagcc attttcacaa
     8341 aatttttctt actactcgaa actaagcgac tttttcacaa cctcctttac ataattttat
     8401 tttgttgtgg aaggggtttc gtgttattct aaaagaggaa cgattgaagg ttataggaaa
     8461 ggaagagatg aatggaacac aaagaactta cctcatttcc aaaagatttt ctgtggggct
     8521 ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga agatgggaaa ggagaatcag
     8581 tctgggatcg atttgtacgg atacctggaa agacatttaa aggaacgaac ggtgatttgg
     8641 cagtagacca ctatcatcgt tataaagaag atattgcttt aatgaaagaa caaggtctga
     8701 aagcttaccg cttctctgta gcttggacac ggatttttcc taatggacga ggagaagtca
     8761 atcaagctgg gctcgctttt tatgagagat tgatcgacga attgattgaa aacgatattg
     8821 aacctatgtt gactttatat cattgggatc ttccacaagc attgcaagat gaatataatg
     8881 gttgggaatc acgtcagatc attgaagatt tcactcatta tgcagaaaca ttatttgagg
     8941 cattccgcgg gaaagtccat tactggatta gtttgaatga acaaaatatc ttcacttcat
     9001 taggttattt gttagcagca catccgccag gtgtgacaga cccgaaacga atgtatgaag
     9061 ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa taaatttcac gagttagaaa
     9121 tgcctggaaa aatcggacca agctttgcgt atacgccaaa ttatccaatt gacagtaatc
     9181 ctgaaaatgt tctggctgct gaaaatgcag aagacctgat ggccaattat tggatggatg
     9241 tctatatgtg gggaaaatat ccgattgctg ccatgaggtt cttagaagaa aaaggatggg
     9301 caccaacgat tgaagctggc gatgcagaat tgctagaatc tgctaaacca gattttcttg
     9361 ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc attaaacgga gttggtgctg
     9421 gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga aactggagtt cctggaatgt
     9481 ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg ggactgggag attgacccag
     9541 aaggattaag gatcggactg cgtcgtatca caagccgtta ccgcatgccg gtactgatta
     9601 cggaaaatgg cttaggagag tatgacaagt tgacagaaga taaacaaatc catgacgact
     9661 atcgaatcaa ttacttacaa agccacgtca aagccatcaa agaagcaatt tcggatggag
     9721 cagaagtcct aggctattgc acctggtctt atacagattt gcttagctgg ctgaacggtt
     9781 atcaaaagcg atatggattt gtatatgttg atcaagacga aacacaagaa ggatctttgg
     9841 aacgatataa gaaagacagt tattactggt accaaaaact gattgaagaa aatggagaga
     9901 acctttagtt cacatactta aaacaagaag gtacggctgg atatggccgt gccttttctt
     9961 gataaaatca tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg aaagaaagcg
    10021 tcagttcttt caaacaaaac tttagttccc ttactttttt ttgtataatg aaacttgatg
    10081 aaatgaggga taataaatgg cacaaaagaa acggcctact aaaaaaagga aaaagacgaa
    10141 aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg acgattggtg ttgggtttgc
    10201 cttgatttta tttgctgtct tcggcttttt aaaattaggt tttttaggaa tattgattgc
    10261 aaacggcttt cggatcattg caggaaatac ttaccaaatc ctctgtcttt tattagccgt
    10321 attaggattt tggattgtca ttaagaatac agagttttca attggtaaaa accgacgctg
    10381 gtttggcggc atactctttt atttcggtat cttattattg ttacatgccc atttatttgg
    10441 caaactccat actggggaac caaatatcat ggggacgaca tgggatttgt tagccagtga
    10501 tataaaacaa agtcaagtag acaataacgt tggtggtggg atgattggag caatccttta
    10561 tcattttact tacttcttga ttgcacagcc tggaagttat ttagcagcag tgctattact
    10621 ggcaggagga gcattcctgt tcagtaatct ggaaggctat cagctgttaa atggtatcca
    10681 atcaattgga gaacgagtac aggaattatt ggaaggtgat cctgccaagc aagctcgaaa
    10741 gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag caaggcgctt
    10801 agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat gagaagaaaa aagcggtgag
    10861 ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca gaaaatacag agccggagca
    10921 gctaagtttt gttccaatcg acagttttca agaaaatatc catcctgcga atctagagaa
    10981 accagtacca gatacaccaa aacagaccaa tacagcagaa ggtttttcag atgagttgcc
    11041 agaggatgac ggtacctcat tagaatttga gattgaagca gaacaggaaa atcaagacta
    11101 tgaattaccg acagttgatt tgcttgattc tataccaact gtggatcaaa gtgatgagta
    11161 taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt tcggtgtcga
    11221 tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca aaattcgaag tccagccggc
    11281 tgtaggggta aaagtaagta aaattgtgaa cttaacagat gatatcgcgc ttgctttagc
    11341 ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa tcactgattg gtattgaagt
    11401 accaaatgga aagatcagta tggtttcctt tagagaaatc atcgaagcac aaccaaacca
    11461 cccagataaa ttactagaag tacctttagg acgtgatgtt tctggacggg tacaaacggc
    11521 tgatctttcg aagatgcctc acttactcgt ggctggttcg actggtagtg ggaagtcagt
    11581 agcaattaat gggattatca caagtatatt aatgcgtgct aagccccatg aagtaaaact
    11641 gatgatgatc gatccgaaaa tggttgaatt gaatatgtac aacggcatac ctcatctgtt
    11701 gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag tagtacaaga
    11761 aatggaagaa cgatatgaaa aattcgcggc aaccggagta agaaatattt ctggttacaa
    11821 tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa catccaacct taccattcat
    11881 cgtagtgatc gtagatgaat tagctgattt gatgatggta gccagcaatg aagttgaaga
    11941 tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg attcatatga tcctagctac
    12001 tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac cttccagaat
    12061 ggcatttgcg gtatcaagcg gtacagattc tcggacgatc attgattcta atggggcaga
    12121 aaagctctta gggcgaggag atatgctatt cttgccaatg ggtgagaata aaccgatccg
    12181 tgtgcaagga gcattcattt ctgaccacga agtagaacgg gtcgttcaat ttgttacaga
    12241 tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg aaacgacagg
    12301 tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg tcgttgaaat
    12361 gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca accgagcagc
    12421 acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag gaagcaagcc
    12481 tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg ataatgacat
    12541 gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac aaggcatgat
    12601 gataccaaaa gaaaaaatgg tttttttcca tcttttctaa tacggtatga tgtcttgtct
    12661 ttttagtttt tgtgagatta aagttctatt cgatttattt gtgaagtatt gcattaagga
    12721 aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat actttggaaa
    12781 aacagtccat ttatggcttg acgaatgaag aattaatcaa ttggtttata gaaaacggcg
    12841 agaaaaaatt ccgagctgcc caagtttggg aatggttgta tcaaaaacgt gtaagtaatt
    12901 tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa attatcggag cattttatca
    12961 tcaatcctct aaaacagatg gtcgtccaag aagcttcgga tggtacagta aaatatttgt
    13021 ttgaactccc tgataaaaac atgattgaga ctgtactgat gagacaagaa tatggcttgt
    13081 cagtttgtgt gacgacacaa gtaggatgta atatcggctg tacgttttgt gcaagcgggc
    13141 tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt tgcacagatc atgatggtcc
    13201 aacattattt tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt gtaatgggga
    13261 tcggtgagcc atttgataat tacgacaatg taatggactt tttgcatatc atcaatgatg
    13321 ccaaaggatt ggcaattggt gctcggcaca ttacagtttc cactagtgga ttagcacaca
    13381 aaatcaagga attcgctaat aacggcttgc aagtcaatct ggctatttcc ttgcacgcgc
    13441 cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca attgagaaat
    13501 tgatggaagc tgtggatgag tatttagaga aaacaaaccg tcgaatcacg tttgaatata
    13561 tcatgttgaa tcaagtaaat gatagaccag agcatgcgca acaattagca gatctattaa
    13621 aagataagaa aaaattagca tatgtcaact tgattccata taatccagtc agtgaacatg
    13681 atcaatattc tcgcagtcca aaagctgatg tattgagatt ttatgatgta ttgaagaaaa
    13741 acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca gcttgcggac
    13801 aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct actcgttttt
    13861 tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt aaataagaga
    13921 aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct catcaagcaa
    13981 agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat tatttaactg
    14041 attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac gtcaagaatg
    14101 gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc atttgttgcc
    14161 atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat gtcggttaac
    14221 gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa atcgaactgc
    14281 tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg aaggtatccc
    14341 tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc agaattatag
    14401 ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga aacgacttgt
    14461 tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt accataagtc
    14521 aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt gtagcctaga
    14581 tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc gccaatacct
    14641 gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa ggtgtgtaca
    14701 ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata gatgacacat
    14761 accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac tggtttagct
    14821 agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac tgcaaacagg
    14881 acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc ataagttgaa
    14941 ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc tacttttatt
    15001 ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt tgtcccatat
    15061 tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata atttggtaag
    15121 aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt aaaattttaa
    15181 agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag acaggataag
    15241 tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa aaacgaacaa
    15301 gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat ttctaccgat
    15361 tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt atatgaaaac
    15421 aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat ctatatcatg
    15481 atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc tattttgaat
    15541 aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa agaatagaga
    15601 tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt
    15661 agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc
    15721 cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag
    15781 acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg
    15841 tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt
    15901 gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat
    15961 tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta
    16021 cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg
    16081 ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag
    16141 gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga
    16201 tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag
    16261 taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa
    16321 ttccaactta tttccgaaga agctactttt ggaacaccgt gtacc
//
LOCUS       DACEDO010000064        16102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEDO010000064 DACEDO010000000
VERSION     DACEDO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.064"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(103..1521)
                     /gene="nhaC"
                     /locus_tag="IWO52_002163"
     CDS             complement(103..1521)
                     /gene="nhaC"
                     /locus_tag="IWO52_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0356414.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1925..3148)
                     /locus_tag="IWO52_002164"
     CDS             complement(1925..3148)
                     /locus_tag="IWO52_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0356415.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3180..4019)
                     /locus_tag="IWO52_002165"
     CDS             complement(3180..4019)
                     /locus_tag="IWO52_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0356416.1"
                     /translation="MKKNIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4036..4716)
                     /locus_tag="IWO52_002166"
     CDS             complement(4036..4716)
                     /locus_tag="IWO52_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0356417.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(4718..5791)
                     /locus_tag="IWO52_002167"
     CDS             complement(4718..5791)
                     /locus_tag="IWO52_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0356418.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(6441..7112)
                     /locus_tag="IWO52_002168"
     CDS             complement(6441..7112)
                     /locus_tag="IWO52_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0356419.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(7200..9878)
                     /gene="mgtA"
                     /locus_tag="IWO52_002169"
     CDS             complement(7200..9878)
                     /gene="mgtA"
                     /locus_tag="IWO52_002169"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0356420.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            10538..10975
                     /gene="ndk"
                     /locus_tag="IWO52_002170"
     CDS             10538..10975
                     /gene="ndk"
                     /locus_tag="IWO52_002170"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0356421.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(10997..11863)
                     /locus_tag="IWO52_002171"
     CDS             complement(10997..11863)
                     /locus_tag="IWO52_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0356422.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(12097..14175)
                     /locus_tag="IWO52_002172"
     CDS             complement(12097..14175)
                     /locus_tag="IWO52_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0356423.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            14668..15885
                     /locus_tag="IWO52_002173"
     CDS             14668..15885
                     /locus_tag="IWO52_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0356424.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
BASE COUNT         4701 a         3470 c         2780 g         5151 t
ORIGIN      
        1 ttcgtttggt taggcttatt accgcaaatg caagcttttg aacaccgttt attcgtaaaa
       61 gggagtgtga caaaagtctt gtcacactcc ctttttacgt ttttattgtt ctaattgcgc
      121 tgtcttgctt actgcttctt gcggtttcac aaatttcttt gagatccccc aacctgtaaa
      181 ggcactgacc atcgaaaaca caggtgaaag caaactgaaa aacacgaatg gcaagtaagt
      241 gagtgtagga atgccaaatg tacttgctac gaaacttccg gcgattcccc aaggcactaa
      301 gtagttgatt actgttccac catcttctag tgttctccct aatacagttg gatcaaatcc
      361 tgctttttcg taagtttctt taaatgcatt accaggcagg ataacagata gaaattgttc
      421 tccaataaag atatttactc cgatgcttgt catcaatgtt gtaaaaatca attttggggt
      481 gttcgtcatc tttcttgata ctcgttccat aacggttgag atcaacccaa attcgatcaa
      541 caaaccacct aatgacaatg ttagaataat caatgacaca gttggcatca tgctttcgat
      601 tcctccacgg ctaagtagtg aatcgatttg ttggtttcca gtatttgaaa caaatcccga
      661 ttcgatgacc cctgccaact tcgttaagga agtttgtggg ttttgaataa agatcattcc
      721 tactgctaca gcaatattca acaaaatcgt caaaattgcc ggtacctttt tccatgcaca
      781 caagagcatt aaaaatagag gaatcagtga ccagatcgaa atagtaaagt ttgcttctaa
      841 aatatcagtc acttcacgaa tagatgaaag acttgcggct ttacttgtgt gtcctaaaat
      901 catgaataaa aagaaagaaa caacaaatgc cggaacagtt gaccacatca tatgcttgat
      961 gtgtttgaac aaatcggcat caacaatagc tgctgctaaa tttgttgatt cggataatgg
     1021 agacatcttg tctccaaaaa cggcacctga aacaatggct ccaacaacaa gggctggatt
     1081 tagtcctagt gttgtcccaa taccgaaaaa tgcaatccca atcgttgaca taacagtaaa
     1141 ggcactccct acagcgctac ctacgattgc acaaacaaca aatacagaag ggacgaacca
     1201 ctggacacta atcaatttaa atccaatgac catgagggtt ggaataattc ctgcttgtat
     1261 ccatacagca atcaatgaac cgactaaaat aaaaataaag attggaatga tccccggttt
     1321 gatgccattg atgatccctt cgtgcagcca atctgtcggg tattttttta tcactgcgta
     1381 caacatgacc attccgatag ctgccaaaat cgtgatatta ggtgaaacct ttaatccgac
     1441 aacgccaatc gcaatgccta aaacaatgac catcaataca agtgttgctt ctaaaaaact
     1501 tggtgttcct tttttttcca tgataatctc tcctttttta gttgttctta tgttctaaaa
     1561 aagttaagat atcggattac tccgccacaa ttcatccgcg attcctcctt tataaaaata
     1621 aaaagacctg ctttcaacta aggacgaatg actccgcggt accaccttaa ttaaaaacag
     1681 atctgttttt cactttcaaa cgattattca aatgtatgga ctttccatgt aattcgtcaa
     1741 ttaggcttga tcggttcaca gcgaccaccg actttctgaa caaaacacct aaccactact
     1801 gcgtgaaaag ttgacttgta tgaagtttac cttccataag atcatttgtc aatgaaaaag
     1861 aaaatatttt ttcatttttc tgaaaattat ttttataact ttcgcgcaca actttatctt
     1921 tttactagaa agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgtt
     1981 tgattttttc taatagcttg ggttctctca ataaatctag agctgtattg gcaagtaatt
     2041 ccgctgcaat cgcaatagaa gcaagtcctt tttcactttt agcagcagct ttgaattctt
     2101 ctgtatgtcc ggcaatatat gtctcagaga ttgctacagt aggctgtatc gttggaataa
     2161 cttggctaac atttcctaca tcagaagaac ctagacttgt tctttgttcc gtttctattt
     2221 cttcatcagg aacccctgct tcttttgcat gttggaagaa taagtcatcg aaagaaggcg
     2281 ttacgatgac atcatcgact gcattttgga acaatccaaa ctcaaaagtc gtacccgtgc
     2341 ttaatgcagc acctcgtacg atattttcca ctttttcata tactttgtct aatgtttgtc
     2401 gatttcctgc acgtaaatag aaacgtccag cagcgtagtc tggtatgaca ttggccgcca
     2461 cgcctccatc tgtaatgatt ccatggatat tgacatcttt tggcaattgc aagcgtagag
     2521 cgttgattcc gttaaacact tggatcaagg cttccaaagc atttactcct ttttcaggtg
     2581 ctgctgctgc atgagaagcg acaccgtaaa atttgatatc gactggatca tttgctaaag
     2641 attctgtcgt tttcccataa cgaaaagctg gatggacaca taatgctgca tcgacatcct
     2701 caaaaaaacc ttcccgaacg aagcttccct tagctgatcc attttcgcct ccttcttccc
     2761 ccggagtacc atacacacga atttctccgc caatctcatc aatgaattgt ttcactatgc
     2821 tggctgctaa tacggagtaa gtaccaaata aattatgtcc gcatgcatga ccaatacctg
     2881 gtaaagcatc aaattctgcc aaaaaggcaa ttgtcggacc tggtttattg gaaatataac
     2941 gtgcatcaaa gcctgttcgg tgcccagcta cttctttttc tacccagaac ccttcttttt
     3001 ccaattgttt gattaaagct gctgaggaat aaaattcata attacttact tcaggatggt
     3061 cgtggatagc tagtgcaagt gcttgatatt ctggtaaacg gtattgaatc agatccttca
     3121 attgatttgt tgagagtgtg gttgtcataa aaatttcctc ctatgctagt cgttttttct
     3181 tattttctcc cgaatgtttc ccacgcaggt acgcttgaac cttttgatgt tttttcgatt
     3241 actttggctg tttcttctgt ttgataggca tctactattt tttgatacgt tttattgtct
     3301 tcatcttctt ttcttgcaac gatgatattg acataaggac gtgcatgctc atcgactggt
     3361 tctaagaaga ttgcatcaga agcgggatta aatccagcat ctacagctac accgctattg
     3421 atgactgaaa tatctacatc tgacaaagca cgtgctgttt gggaagcatc taactcagaa
     3481 atgtctagat tttttttatt ctcggtaata tcagaaacgg taggcgcttg gccttttgcc
     3541 ttgtccactt tgatcaatcc agcagtttgc aataacagaa gagctctgcc gccattcgtg
     3601 acatcattag ggattgcaac ttttgctcca tctttgatat cagccacatc ttttaacgta
     3661 ttggaataaa ttcccaacgg tgcattaacc gtattcccaa tcgatactaa atcagttccg
     3721 tgttcttcat tgtaattgtc taaaaagaat tgatgttgga aggaattcaa atcgatcgat
     3781 ccatctgcta aagccgcatt tggctgattg taatctgtaa atttcacata ttccaaatgg
     3841 atgttttcct ttttcaaacg gtctttcaca tcgtcccata catctgtatc ttccccaatg
     3901 atccctagtt ttacggtcgt ttcttttttt tcagttgtag ctgcttttcc ttggctgcat
     3961 cctgccaacc aaataattgc tacaaatgta atgactgcaa aacgaatatt ttttttcatt
     4021 ttttctcctc cattttcaat gtgtgacttt tttaatcagt tgattactga taaattgact
     4081 caaaaaaact aagaccagaa tcaataatgt agcgacaatc gttacatctg tttggaatcg
     4141 attatatcct ctcgatatag caagattccc taatcctccg gcacctactg ctccagccat
     4201 ggtagtcagt ccgatcacat tgatgatcgt caacgctgag acccgaatga taccaggtaa
     4261 cccttctaac aaataaacac gtaaaatgat gccgattggg ctcgtgccca tcgcttctgc
     4321 tgcttcgatc accccaggat caacttctag cagtgcattc tcgatttgac gggcaaaaaa
     4381 cgggacaacg ccaaaaatca aagggactaa agctgctttg gcaccaatcg tcgttccaac
     4441 taagaaacga gtagtcaaag ctaaaagaga aagcaagata atgaaaggga tggaacgaat
     4501 gatattgatc aacttatcaa gtatttcgaa cagcagcctg ttagctagta atccatttgg
     4561 acgtgtcact actaacgtca cacctaagat gattccgatg atcccaccaa tgaatgccgt
     4621 ccaaaaagac atatatagtg tttgctttgt cgcttcgatg aattcatccg gaatcaaata
     4681 gacattcggc aaatatgttt taaaaaattc agtcatttta gtttcctcct tcgatgactt
     4741 tgatacggtt atctaattca tttttttgta aaatagttac ttttacacca gatttaatca
     4801 aataatccaa tgctttttgc cgctgtttgg tttctccagc taaagtaacg atcaaacttc
     4861 ctaagggtgt ttcttgtaat agctctacat taccgtataa gatattcgct gttacttggt
     4921 acttgctgta aagatgagcg attagaggtt cgttcgtctg gttgccgata taacttaatt
     4981 ccaccaacca tttatttgca atctgatcgg caaaagcagc atgggcactg attgtttcca
     5041 atgcttggtc aaggtgtgtc gctgtccgaa taaaatcttt agtcaattct tcttcaggat
     5101 gactgaaaat ctggatgctg ctaccttttt caatgatttt ccccgcttcc ataaccgcta
     5161 ccttgttgca gatttctttg acaacttgca tctcatgagt gatcaggacg attgttagac
     5221 ctagctgacg atttagtttt tttaacagtg caaggatctg aagggtcgtt ttcggatcaa
     5281 gtgcactagt tgcttcatcg cataacaaca ctttaggttc atttgccaga gctcgtgcga
     5341 ttgccactcg ttgtttttgc ccccccgaca gttgtttagg atacgcagat gctttttctt
     5401 ccagaccgac aagttccagt agttcattca ctttttgacg acgttcctgt tttgattttc
     5461 cagaatactt tagagaaaaa tctacgttgt caaaaatcgt tcgactttcc attagattga
     5521 aatgctgaaa gatcataccg atggcctttc tttcttttcg caattctttt ggagagagct
     5581 ggcttaaatt cgtctgattg acgatgactt ctcctgctgt tggtttctgc aacaaattca
     5641 tcacgcggac taacgtactc tttcctgcac ctgagtaacc gacgatccca taaacatccc
     5701 ctttttcaat cgttaaatct acagaatcta ctgcctgtat ctctttttct ttttgtttga
     5761 aacatacaga aatatctcgc aattcaatca ttaacattcc tccttgtcta taaaaaaagc
     5821 atcctcaaga aaatgaaaat agtcattttc ttgaggacgc ctttttgcgt gttaccacct
     5881 catgttcaag ctgtgttcac acacaactcc tcttcaagta ccatcatact ctagcaagat
     5941 atcggtcgca gccgtcaccg ccttacatct atgctcggga tgaattgctc gatggccatt
     6001 ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa aggttcttct
     6061 atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aaatcctttt actaacataa
     6121 actatgtcag taaaaggacg acgtatcgtg gtaccacctt ttttcggaag tttccttccc
     6181 tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga acatttccga
     6241 atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc ttacttgact
     6301 cttcacacca gccagagcct ctctgcaaag tttccaagct tactttcctt ctcaacgaaa
     6361 tcaatatttt gttgttggga ataatctaca ggaggaaaac tgaaaagtca agcatgaaat
     6421 ccaaattatt ttttctatat ttatttttct gtttttttat ctaacatttc aataacagtt
     6481 cgaagatctt ttccttccgc aagaaggttc aatttttcct tctcttcgac gctataattt
     6541 cgaacaaaac tggaatcggg aatcaaaaca acgcctagtc ctgcacgtga agcggctgtc
     6601 gctcctacta aagaatcttc cactgcaaaa gctgaaagag cttctaatcc tgttcgttcc
     6661 aaagctgtca gataagggac tggtgaaggc ttgttctccg atacttcatc tccgaacgta
     6721 aacgtatcaa agtaatcggc taattgaaag tgatccaaga tatctgttgc tcttttcgta
     6781 acagttgtcg tagcaatccc tagtttcata tgacgttctt ttaagtctgt caatatttct
     6841 tttgcatagg gtttcaattg gatagctcca cttgtcagct gttgataaaa atattcttca
     6901 cgctttgctc ggacttcctg cactttttca ggactgcctg ctgcctgcac taaaatatca
     6961 aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttctttcgg gatatgaata
     7021 tcaaattgct ggaatcccca aagccaacca tcccgatacg ttctttccgt atccactaac
     7081 aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc caaaacaata
     7141 cacctagttt agcatgactg atccaaaatg aacagaagca tagagcccag ccgcatcact
     7201 tataagagtt cttggaatcg acggatatag attttcttca cagctgtcac aagcgccaga
     7261 taagcgacga ctgttataaa caaccataac cagtaattgc cagggagcgg catcaagcct
     7321 aactcagcac caaatcctgt gaatggaagg actgaaccga caatgattcc taatgtagtg
     7381 ataaccagca tggcaaatgt tgcattgctt tggataaatg gtattttcgg tgtacgcaat
     7441 gcatggagta ctaatgtttg tgtccacagg gattcgacaa accatcctgc atggaataat
     7501 gcgataaaaa tcacttgttg tgagctatcc aatgtatgga aacttccacc aactacagca
     7561 ggacaaataa caaaatacat caacaagtaa gtagtgatat caaaaatcga acttgttgga
     7621 ccaaaataaa ccatgaattt tccaatacta gatgcattcc atttctttgg ctcatccaaa
     7681 tattctttat ccatccgatc ccatggtaag gaaatgcaag aaacgtcata gatcagattc
     7741 aaaaatagaa gttgcaaagg caacattggt aaaaatggta agaacgtact ggcaatcagt
     7801 acggaaaaca tattgccaaa attagaactt gctgttgcct tgatatattt catgatgttt
     7861 ccaaaagttt ctcttccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa
     7921 aggatcacgt ctgctgattc ttttgcgata tccacagctg tatccacgga aataccgata
     7981 tcagccactt tcattgctgg agcatcattg ataccatctc ctaaaaatcc gacagtatgc
     8041 ccatttttac gtaaagctgt cgtcaatcgt gctttttgct gaggattcaa ttttacgaat
     8101 acttcgtgtt cttcagctac ttgcgctaat tcatgatcat tcatttccga gattttctca
     8161 ccagtaatca attcattgat ttctaggcct acttgacggc acacagaccg tgtgactaat
     8221 tcattgtccc cagtcagaac ttttacagct acaccatgtt cttttaaagc tttcaacgct
     8281 tgttttgttg tttcttttgg tggatcaagg aaagcaagat aaccaatcag aaccatgtct
     8341 gactcatctt tgactgaaaa ttctccaaca acgcttggat tggtcttttg tgcgacacca
     8401 atcactcgta agccgtcttc attaagtgat tgtacttttg caagcaccgc ttcttttcgt
     8461 tcttttgtta atggtgcgac agtaccacca agatcaataa atttagaaat gttcaacatt
     8521 tcttcaatcg cgccttttgt gatcatctgt gttttgccag ccggatcagc gactacaaca
     8581 ctcatacggc gacgttcaaa atcaaaagga atttcatcta ctttttgata attgattgta
     8641 tctgtatcta aagtttctgc tgcttcatca atgatagcca catctaacaa atttttcagc
     8701 cctgtttgat agtagctgtt tagataagca tggcgcaaaa cacgatcatc ttcatttcct
     8761 tcacaatcca tatgatattc aagaatgatt ttgtcttgtg tcaatgttcc cgttttatcc
     8821 gtacataaca catcgatcgc accaaaattc tggatagcat ttaaattttt gatgactgtt
     8881 ccttttttgg ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc
     8941 atttcaggcg ttaaaccaac tgccacagat aatccaaaaa gaaaagcttc taaccaatct
     9001 ccttttgtaa gcccattgat aacgataact gctggagcca tcaatgacat aaatttgatc
     9061 agcagcatgg atgttttgtg gatgcctaat tcaaaacttg attggatggg tttttcagat
     9121 agcgtttctg ctacatgccc aaatagtgtg tcatttccga cagaaacaat gattccttcc
     9181 gcgcttccac tgatgacatt gcttcccata aagacaagat tttcataact tgtttcactt
     9241 tgctgatcgt cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat
     9301 gctgcttgag aaatgaatag atcctttgcg gacaataaac gcatatccgc tggtatcata
     9361 tctcctgctg aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgta
     9421 tactcgccat ttcgtttgac agctgccgtt actttcacaa gtgatttcag tttttcagct
     9481 gcttgattag aacgcacaga ctggatcaac gtcatcgtcc cactgacgat aaccatagtg
     9541 aagataatgg ctacaccaaa gagatcttta tctcctggag cggctaatac gtattccgta
     9601 atgaatgaaa taattcctaa tgcaatcaac actaatgtaa agggtgtgat gtatgctttc
     9661 aatatttcta taatgaatgg tgtttttttg ccatatgaaa tcgcattttc accataagtt
     9721 tctcttgaac gctcgatttg ttcttctgac aatccatttg gtgatgcatg gaacaattca
     9781 aataattctt gcttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt
     9841 ttttccatct cgatctctcc tttgtgtcca aaagacagct gttctcgctg tttttggtct
     9901 tttgctattc atcggatcga tagagaaaat gctgaaaggg gctgagaaca gatagataat
     9961 agccctggcc tttcctctaa atccttgttt ttcgaatgac tgtcatccat ttgttctcat
    10021 ctcctttact aagtaatttt aattcatgac ttccaatcat ttttcgaata gttattttcc
    10081 caactcgctc acctcccttt ttgttttttc acctatggga tttgcggcaa caaaaaaacc
    10141 tctcgccaaa tactcagcga aaggtttgaa aaagcaaatc aaaaaaagct tctcagatct
    10201 catcgttgag ttttgacact gtacaacgta aaaagcagat atctgctttt agctacctta
    10261 tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga cgtttttggg
    10321 cagtagccta tgtttgtata ggagcctcac ctaacaaatc ctattcggtt ttccctcgtt
    10381 aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca tactttttta
    10441 aaatacacgc gttatatttc ctgtctcacg cagttttatt tgtctcattt tccacctccc
    10501 gctataatag aattatagta ggaaacgagg tgctgttatg gaacgaacac ttgttattat
    10561 caagccagac ggcgtaagac gccatttggt cggaagcatc atccaacgtt ttgaagcaaa
    10621 gggactggcc attgctgaaa tgaaatttgc aacgatgaca cctgaactag caaaagaaca
    10681 ttatcagcac ttgactgaac gtagtttttt tgatgagctt atcgattaca tgacttctgg
    10741 tccggtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt
    10801 cggagcaaca aaaggggcgg atgccgttcc aggtacgatc cgaggagatt atgcactgcc
    10861 ggggactgaa aacatcatcc atgcttctga ttcccgtgat gctgcaacaa aagagatcgc
    10921 ccgctttttc ccgccattcg atacaaacaa accgtctgaa aaagcggcga agtgatacaa
    10981 atcctaaact gttggtctaa ctgctgggtt tagcaatggc cagcagtttt ttctctaaag
    11041 aattcctttg attcttccct aatgtatatt cgatcactgc gaaaaactct tggtgatttt
    11101 ccgatttttc ttcccctaaa gcagcagaaa tcagtcgttg gtaataatcc gccatcaatg
    11161 tttcataaat cactgtactt ctttctttat gcgctaaact ccgtttcata tcttcattca
    11221 cgctacgtgc cttttttaag cgaccatttt ctatataaaa caaagtcaag ttcgtcaaaa
    11281 aagtaaagag atactgctcg tgattattca gttgcgcgat ccgtcgcatt ctttttttga
    11341 ttttcaaata attattctca ataaaacgat cactgaatat ccaaggaaaa tctgctagaa
    11401 tagatacttc aaattgccca tactctggta cttgcatcaa atattcttca atgatacgac
    11461 attcataacg caaaaattca aattccgtct cagatacttc tgtttttttg gacatgtgca
    11521 gattcaaaac caaatagtac agatcttctg ttttcttata gtgcctgcgg ttattagcaa
    11581 tttctttgta aggatcaaag ctgtcttctt gaatccgatc tagcattcgt ctttttttct
    11641 ttaatttttt tggctcatca agctgatttc gtatccattc atattcggca agtgtcatgt
    11701 tcattcgctc taaaaatgat ttcaatgttc caaacgatac ttttttcact gaatttgtct
    11761 cgatcccgac tagcgaagtt ttagaacaga ttccttccgc taattgggtc tgtgtcagct
    11821 ttttgttgac tcgaagcttc ttaatcaatt ctccatcata cataatagtt ctcctttttt
    11881 aattgaaatt aacccttggc ttttccagaa tgcttgccaa taagaggaat gattatttca
    11941 atttaaattt tccgcattca ataaatagat acgttaaaaa taaaaaatac acgaaaaaaa
    12001 taaaaaaatg tttttctcat aaaaaatacc aaccttcaag catgagactg ctgtctaatc
    12061 cctgaaggtt ggtttgattg tttattgatc atttttttac tgctccgcat tggttcctgt
    12121 tactggatat aggatagtcg ttgttttatt gccgtttttt gaaaagacac tggtcgaatg
    12181 ttcacctttt tgcttttcga ttgcttcatt acgttccgct tcgtttttat agtcatattt
    12241 tttcggatca atcgtctcaa atccgtttgg cacataaaac cgcaagagat tttcctgatt
    12301 cagcttatct gaaagactta acgcagtctt gactttttcc tgatctttgg agatttcctc
    12361 gtcgattcct gctgctttcg tatcgatgat ttctcctgtc tgattattgt aagcttttcc
    12421 tcctaggacg gtgtatttgg gcgtcacgaa gttcccattt cgaaaagcaa ctacttgatc
    12481 gtgctgagaa gataataagt ctgtaccaaa atgaagataa tctttatcat cgattcccaa
    12541 tagatgtagt aatgtcggca aaacatcgat ttctccacca tattcatgat tgactgtccc
    12601 tttagtgtaa ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa
    12661 ttcatcccat gtatctggat ctttaccaag agcacttgct aaatctttat tatctgtatt
    12721 cgagatccca aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa taccagattt
    12781 tttcaaatac gtaaaaaatt gttccaatga ttgatccaga taatgggccg ttcgaacata
    12841 attatctact gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt
    12901 ataataaggc gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg
    12961 ttcaagatat tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt
    13021 ttcatcggat tcatcaaaat aagatcgatc aaagaaattg tcatacccta aatttttata
    13081 tacatgatct ctattccaga aactagctac attcccatgg aaaacagcac ttgtatagcc
    13141 ttctgtttga ccaagggtcg ctggtgctgc ttgaaagaca ttatctgacc caagttctgt
    13201 gaataatgag ccttgaggta aaccaaatgt gccagtttcc aacatgtttt cagcatcact
    13261 tgtctttccc tgtcctactt catgaaagaa attgtcaaaa ctgatcgttg cttgattctg
    13321 gaaaatacta tttaaaaacg gcgtgacttc ttgtccgtct accttcatat tgatcaaaaa
    13381 ctgttgaaaa ctttctaaat gaaggacaat cacatttttt cctttagcaa tcccaaatgt
    13441 ttcaggattt ggctctgcat aatgtttctt tgtatagtct aaaacttcat caattccatt
    13501 actgcttgca tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt
    13561 gtacgcatcg attcctagat atttgacaat ataggaacgg tcaaatgtcc gctgtagcaa
    13621 ttggggacgg ttggcttcgc tcaacgctaa atttcctgaa aaaatcaatc ctgaaagaca
    13681 ggtaacagcg atagccatcg gtttgctgac agggttgctt ttgataggta ttttcttgac
    13741 cttcaaccaa accagtagcc caatgaacac agcgatatcc aaccaataga tgacatcatg
    13801 gggtttcatc aaagaaaagg agcttcctga caacccttgt gacactttcg aaaagcctaa
    13861 tacagatttg actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa
    13921 caaaagcgta tttaaaatat caatgatcaa aagaatggct aacgctggct ttggcttttt
    13981 aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca atcgggttga tccacaagat
    14041 aaagtactgg atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt
    14101 cttcagccag aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt
    14161 tttaagttgt gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg
    14221 aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat
    14281 tattccattc atttttcaca gcataaaatc gtttcggtgt aagcggttaa aatagtaagg
    14341 agataaaaat cttgtggtga ccgatttact ctatagtatt caaaagtagt ttctcggtat
    14401 tcgtacaaaa aaaacatact aagattagag gggaacatca ttgacgaatg atgttctcct
    14461 ctattttcta tttttagatt atagtgaaga gtgaaagaaa gttcgaacaa agccttgagg
    14521 actacgagtc cctatcaaaa accgaacttc tttactgtca ttttagaatc aggtttacgt
    14581 atgttggggc catgagcccg tgtataggaa attggaatcg aaatgaagca gtaaagttct
    14641 ttcaacactt aaaaaggagg aaaacaaatg ccttacattc ttgcattaga tgtttcaatg
    14701 ggacacagct acagtgtcct atacaaagac gacacctgct tatttgaaga cgagatcgaa
    14761 cacactcaaa gaggatttca tgcattactc gaagagatcc agcacttgcc tgaacgtcca
    14821 ttgattgttt ttgaagcaac gggtatttat tccagaccag tcgaaaagtt ctttcaagat
    14881 catcactttt cttactgttt gttaaacccg ttagaagcga aaaaacagat ggaagaaaac
    14941 accttacgca gttggaaaac agataaatcg gatgcccatc ggctcgcaca gactcatctt
    15001 aaaaatcaac gtcaaccaaa agaagtacaa agcaattttt acttagagat gcgcgattta
    15061 gcccgttttt atcaagaaat tgagaagaaa atcactcgac tgcgcatgga tttacacaac
    15121 tgccttcagt tgacttttcc agagcttgaa cagttctttt caaacaggct gaccccttac
    15181 gcattgacat tgatccgtct ttttccccat ccagattttg tattggcatc tactcggaca
    15241 aaaataaaaa ataaactgat caacgaaaca cgggaaaaaa tctccgcgaa tcgtgccgag
    15301 caaaaggcag atcaaatcat ccactatgca cagtgtgcct atccagctgt tgaaaaagac
    15361 agtattcact gtcaaaaaac gatctattac gccgaacttc tccaagattt actcgaacaa
    15421 aaaaaagcac tggcgacaca aatgatcaaa aaagccgaag cctctccttg ctttttgttg
    15481 taccaaacgt tccctgggat tggcgcactc acagccgctt tattgctcgg tgaattagga
    15541 gatatcacac gatttaaaac ccataaacaa ttgaatgcct ttgtcggaat cgacatccga
    15601 cggtatcatt caggtaagta tactggccaa gaccgcatca ataagcgtgg taatcccaaa
    15661 gcacggaaaa ttattttctt caccattcga aatatgattc gccagcaacg cgcagctcct
    15721 aatcatattg tcgattatta ctacaaatta aaaaagcaac ctatccctaa gaaagataag
    15781 gttgccacag ttgcttgtat gaacaagctt ctgaaatgta tgcacgctat ggttagggca
    15841 catacagagt acgactatgc gtacgcggtc tcagtggacc attaactaaa ccacttacag
    15901 tataccactt ctttgcccag atcgaaaatt ttttacgaac ggggctttat ttagtgtgcc
    15961 ttgattttgt cgctttcgtt tacgagctat ttttcttgca ccttttactt gactaatcgt
    16021 aggaaagagg ctgtgacaaa attcttgtcg cagctcccta tttcttgatg caggacagct
    16081 ttttcacaac ctctccacta aa
//
LOCUS       DACEDO010000065        15299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEDO010000065 DACEDO010000000
VERSION     DACEDO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.065"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(123..647)
                     /locus_tag="IWO52_002174"
     CDS             complement(123..647)
                     /locus_tag="IWO52_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0356425.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IWO52_002175"
     CDS             complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IWO52_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0356426.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IWO52_002176"
     CDS             complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IWO52_002176"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0356427.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2369..3049)
                     /locus_tag="IWO52_002177"
     CDS             complement(2369..3049)
                     /locus_tag="IWO52_002177"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0356428.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3175..4044
                     /locus_tag="IWO52_002178"
     CDS             3175..4044
                     /locus_tag="IWO52_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0356429.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4070..4585
                     /locus_tag="IWO52_002179"
     CDS             4070..4585
                     /locus_tag="IWO52_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356430.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4632..5195)
                     /locus_tag="IWO52_002180"
     CDS             complement(4632..5195)
                     /locus_tag="IWO52_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0356431.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5250..5429)
                     /locus_tag="IWO52_002181"
     CDS             complement(5250..5429)
                     /locus_tag="IWO52_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356432.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5506..6015)
                     /locus_tag="IWO52_002182"
     CDS             complement(5506..6015)
                     /locus_tag="IWO52_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356433.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6111..6491)
                     /locus_tag="IWO52_002183"
     CDS             complement(6111..6491)
                     /locus_tag="IWO52_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0356434.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6821..7570
                     /locus_tag="IWO52_002184"
     CDS             6821..7570
                     /locus_tag="IWO52_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0356435.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSRGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7754..7921)
                     /locus_tag="IWO52_002185"
     CDS             complement(7754..7921)
                     /locus_tag="IWO52_002185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356436.1"
                     /translation="MKDIQSGNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRFN
                     DEARKYLKELL"
     gene            complement(7937..9031)
                     /locus_tag="IWO52_002186"
     CDS             complement(7937..9031)
                     /locus_tag="IWO52_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356437.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSK"
     gene            complement(9110..9754)
                     /gene="pcp"
                     /locus_tag="IWO52_002187"
     CDS             complement(9110..9754)
                     /gene="pcp"
                     /locus_tag="IWO52_002187"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0356438.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9953..10897)
                     /locus_tag="IWO52_002188"
     CDS             complement(9953..10897)
                     /locus_tag="IWO52_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0356439.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10899..11585)
                     /locus_tag="IWO52_002189"
     CDS             complement(10899..11585)
                     /locus_tag="IWO52_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0356440.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12058..12378
                     /locus_tag="IWO52_002190"
     CDS             12058..12378
                     /locus_tag="IWO52_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0356441.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            12539..13444
                     /locus_tag="IWO52_002191"
     CDS             12539..13444
                     /locus_tag="IWO52_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0356442.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13441..14214
                     /locus_tag="IWO52_002192"
     CDS             13441..14214
                     /locus_tag="IWO52_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0356443.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(14391..>15299)
                     /locus_tag="IWO52_002193"
     CDS             complement(14391..>15299)
                     /locus_tag="IWO52_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0356444.1"
                     /translation="AISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVF
                     VGIEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPA
                     MGYVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNK
                     NGAPTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQR
                     KEWVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHL
                     DKILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         4475 a         3160 c         2721 g         4943 t
ORIGIN      
        1 agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc
       61 ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc
      121 ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag atctgttctt
      181 gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct
      241 ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc
      301 caactgatga tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga
      361 atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat
      421 gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg
      481 tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc
      541 aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca
      601 tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca
      661 tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac
      721 ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg
      781 cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata
      841 ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa
      901 cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg
      961 cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc
     1021 accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg
     1081 ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct
     1141 ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca
     1201 gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct
     1261 gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc
     1321 ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca
     1381 attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca
     1441 gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat
     1501 tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca
     1561 actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct
     1621 acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc
     1681 aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga
     1741 tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg
     1801 atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt
     1861 ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc
     1921 ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt
     1981 tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct
     2041 ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt
     2101 tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa
     2161 acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga
     2221 tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt
     2281 attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac
     2341 tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg
     2401 gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa
     2461 agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct
     2521 tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt
     2581 tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc
     2641 gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct
     2701 gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa
     2761 gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag
     2821 ataagccatg agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa
     2881 cttcttcata aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct
     2941 tttgggacgc atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa
     3001 attctgagga caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct
     3061 cctaataaaa agaataactc acattttctt caatcacaag ggacaatctt caccctcagc
     3121 acttgattaa tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc
     3181 aaattaattg catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct
     3241 gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact
     3301 ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg
     3361 atcactttga acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg
     3421 attccaaata atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat
     3481 gaagtagcaa ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt
     3541 gcttccatgg tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc
     3601 tcagctaagc tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa
     3661 gaaaagaatc tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta
     3721 ggtccagttg gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa
     3781 aacaatatcg aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct
     3841 cacgagcgtg ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc
     3901 agcatgctgc aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa
     3961 gattatgcca aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg
     4021 cgcgcgattg cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt
     4081 tttcattcag gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg
     4141 acattccctg tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta
     4201 tgccatgaat ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg
     4261 ttttgagctg tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc
     4321 cggtgacttt tattatattc gtagacttca ctggacagtc caaggtgata tctataatca
     4381 tcaatatcaa atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt
     4441 ttcaggagat tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg
     4501 tattgctgcg gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt
     4561 ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca
     4621 ggtacatctt tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc
     4681 tgaccgacaa ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca
     4741 tccacactta gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct
     4801 aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa
     4861 taccattcgt tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga
     4921 tatttctcta agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa
     4981 ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg
     5041 taacgataat ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct
     5101 gtctgcttca aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt
     5161 tctggagcat cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct
     5221 aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta
     5281 atgaaataac catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact
     5341 ggcatttctt tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt
     5401 ccgcaattag ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc
     5461 ttagcttact cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca
     5521 tacctgacgg tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt
     5581 gactagaagt gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt
     5641 catcgctgct tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc
     5701 ggccttttcc gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac
     5761 ttgtccttca caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt
     5821 ttcaactgca aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc
     5881 tgctgctttt ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc
     5941 ggcattgagc agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa
     6001 catcattttt ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga
     6061 acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc
     6121 agttctaatg gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta
     6181 tcttctctaa tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca
     6241 atatcgtcta ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt
     6301 tgtggcgcat tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat
     6361 ttatagtcat ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta
     6421 acataaaaat cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt
     6481 gtaaggttca tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag
     6541 ttccattgta aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa
     6601 ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat
     6661 gaaagtaaga aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt
     6721 tataattgaa agactagttt tttttcaaga aaagcataca atagattgtg ttagtaaata
     6781 cagaacacac tacatttagt gtcttaagga gtcctcatac atgaagtcat attttcagtt
     6841 tttagctcat cagctaaaag gctggccgca acaaaattat tatcttttct ttttcagtcg
     6901 tggctgccag gtcatgactc ttgtcaatag tcccatcact tggctgtccg ttattacatt
     6961 tatcggtacc acgcttggtg tattgtgtat tttatccatc aatgcggcaa aatcagtcaa
     7021 cggtatttta ggtattttgt ctgcgatttg ttttattatc gttgggtttt cagcaaagaa
     7081 ctatttaagt atcggtgaac agattgctta tatgatcaca ttggatatcc ccgtattgtt
     7141 aagtaaagaa tggaatcata atatggcttc aaaaatccga aaattaactg gaaaaagctg
     7201 gtttatcgca atcatcagta ctttccttgt ctatcttgtt tcaggattcc tgattggtcg
     7261 ttatacagat gatcctcggc cgtgggtaga tgccattgct ttttctatta gcttgactgc
     7321 tggtatcatc agtttcttgc gttacaacaa ccaatattac tggtggttag cttcagggct
     7381 tgctcaaatg gtcctttggt atatttcatt tacgcatgga tctgcaagtt tggcgatgtt
     7441 cgtgaacagc tctgtttact tgctgaatga tgtccttgca tttaccgttt caccttggta
     7501 taaccaaaaa gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca acgcacaaca
     7561 acacgcataa aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
     7621 aacacgtata aacggcggaa tcagaagcaa ctccttcgga aataagctga aattcacaaa
     7681 aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc
     7741 cagcctctat tttttatagt aattccttca gatactttct tgcctcatca ttgaaacgca
     7801 gtgattcttc taacttctga taatattcca acgcttgttt ctgagcttca gtaaaacgat
     7861 aatgttttcc tttccatttg tcgattgtct cttgcacatt tccagattgg atatctttca
     7921 ctactagttg ccatttttat tttgagactg tacggtcttt cttatcagca gattgtatag
     7981 cttctcgttc tttacgcaat acttttaaaa aaccatttct gactatccga ggaatgctgt
     8041 cgaagaaccc ttctgtaaaa ccatagcgac aatattgctt ccaaataaat agatagattt
     8101 ttggttggat gaatcgttgg tttttcgcaa aatattccat ctgagcagtg acttctggtg
     8161 ttcgttgacg ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa gcattaggat
     8221 gtactggcac ttcttcatat acgatggatt gccaatcttt acgtaaaaat gcgcgatagt
     8281 tgatcaagaa ttcttctaca tcctttacta attcttctgc aaagtcgtca aatagttctt
     8341 tttttatctg tcgctttttt gactgcccat tgatgtgttc acttattttc ttgatttcat
     8401 tttccaatgc ttgataatcc gctatgtaaa tcggcatttc tacttggcga aattctgcga
     8461 gccgctgagc taattttata tccggtaaat atggctgttt catccgacga ttttggtaca
     8521 agacaaaaca gtatttttgc catagtcctt caggaaaatc gatattcttc tgcttaagcc
     8581 ataataattc gtctaaggcc agatcaactt ttgtcattcc ctgatggatc gaagctgcca
     8641 ctgtcggaga gacatgcagg tgatcggcaa agtggttgat gcctagtttt aggatctttc
     8701 cggtttttgg cgatttaacg atgtattgca cttttaattc tctaccacac tcacaataca
     8761 atttttgctg gcttcgtttt gttcgaaaaa acggattgat cttgaagtca gaaaacaccc
     8821 aatctgttgc agctaaataa tgttcttcca taaaacgaga gctgattaga tatcggcggt
     8881 gcttatcgac tagttcccgc tgttttttcg ttaattttgc atagatctcc caacgttgac
     8941 tgcttgtgta ttgtttagaa attttgatgg gatagctttt gctgatttct tttggaaacc
     9001 attcttcaaa gacggattgc cattcttcca cggctgtttc tttcctttct aattacaaaa
     9061 agctggctac taacataacg ttagcaacca gccagtgttt cttgctaaat tagtgagttg
     9121 ttccggcatt tccggaaata tcttctttat tttctactgc tgcttcaact gcataagtca
     9181 aagaagaagc aatgtcttct aaagacatag aaggcgtacc cactggacga tcgatcactt
     9241 gatcgggtga gaatggcacg tggatgaacc cacctcgaat tgccgggaat tgtgtttcga
     9301 tcaaatgaag caaacgatac atgatatcat tgcagacaaa tgttcctgct gtataagaga
     9361 tgtacgctgg tagaccatga tcttgtacat tttttgtcat tgctttgatt ggcaatgagg
     9421 taaagtaagc ggctggaccg ttttcttcta aagccgtatc gaatggctga tttccttcat
     9481 tatcagggat tcttgcttct gctagattga ttgcgacacg ttcgaacgag actgctgacc
     9541 gcccacctgc ttgtccgaca cagatcacga catctggttg gtgctgttgg atggcttctt
     9601 tgactacttg actgcttttt tcgaatacgg tcggtacttc cactttgatg atttcagcac
     9661 cgctgatttc atctggcatt ttttttactg cttcataggc tggattcact ttgtccccac
     9721 caaatggatc aaaacctgtt actagtactt tcatctgttg ttcctccgtc cgattgattt
     9781 aacatatggt tatcttttat cataccattt tccttgaaca gcgtaaataa aggaaacaat
     9841 agtttaaata acataacaga tcaaacttaa tatattctta taaaaaaata gtgttccact
     9901 acaatgttag aagaggttgc gacaattatt ctgtcacaac ccctctcttc tattatttaa
     9961 atagaatcat ataagcaatt tggaaaacta gcataaataa cgcaataaac aactggtttt
    10021 tgatcactcc gtatttgtct ttcatctcca gcatcgctac aggcacgata ttgaagttag
    10081 cagccatcgg tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc attcctatta
    10141 acgctggatt cgctccatga ctaaatacga atggtgcacc gatcccaacc gtcatcacgg
    10201 tgatggccgc aaatgcattc cccatgatta ccgtaaatat caccatgcca agagcgtaaa
    10261 tgatgatgcc gatgttcaca tttccttctg gaacgatcgt acctactgca ttggaaatca
    10321 ctgtcccaac tccagcgctt gtaaataccg cacctagaga agctaaaagc attggcaaca
    10381 tgcttaacgg ccctactgtg cccagcatat ctgctgcatc atctaaaaat gtacttggtc
    10441 gattatctcg agaaaagaac atcaagatta gcatggctgc aaaaacaccg acgcccacgc
    10501 cgactaaagc acctaaattg gtaaatagag caaacaagat cgcaaagaca ccgatactca
    10561 aagctggaat aaaaattttc attcctagtt tgtctgacat tttttccata tattcttttg
    10621 atggaagccg gctttctcct ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa
    10681 agatcaagcc accgttcacg attgccggaa gccatcttcc tcctgcaata actaccccta
    10741 agaccgtcca gaaaaaggct gacccataac gatgaggatt ggttttatct gttaaatttc
    10801 ttactccagt gtaaatcaat acgagtccca tcacgatata aaggatctcc agtaatttat
    10861 cgcctaataa aatatcagga tttgtaaaaa agtccatctt attgcccctt tcctggataa
    10921 tatttcttcg ctaatttctt ttctttaaga tagaagtaga cacttgccac aactaaagcc
    10981 gtgatagcca ctggaacttc cactaaagca agctgtccca atgttacgtc atatcctaga
    11041 tctttcagtg ttgattggac tagcaacgcc cctgctcctc cgatgaatag tacttggcca
    11101 aagaaccagc agacattttc catagctgaa gccatacctt ttagttcttc ttcatgctta
    11161 ggatggactt ctaacccttt tgattcgatg gtccccaatg ccatcggtaa aatgattgga
    11221 cgaacaaatc cagcaactcc accaaaactc acgttaaaag ccgcaaagat cattcgaaag
    11281 atcccataaa tatctacaat cataccagca gatactttct tgaatcgctt gatcaaggaa
    11341 gccgctgatt ctttcagacc atttctttca agcgttcctg ttgccaacat aatgatgata
    11401 aaaatcgcca tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa cattccttga
    11461 atgccaagac cgccagtcaa agctgtaaca actaaagcaa ccattacgat caatatcgag
    11521 tctaatttaa atgcaaaccc gagaataaca atcaagacgc ctaaaaggct aagataattt
    11581 tccatatatt ttcctccata aaatgacaat atttttttaa ttataaagca aaacacataa
    11641 aaaatatagt tgttttctca cgaaatagac actattttta aatatttttc gttatttttt
    11701 tattatataa ttcaccttac ttattgtaag ataaagcttt ttttaattta ttgcctgcta
    11761 atatataaaa ataaggtcta gttactgttt ttttaaatga ttaataaata acaactcctt
    11821 tctatgaaat tgcaaagcat tcatcttctt ttctgctaac gatatatccg tcaatgcttg
    11881 ttacacttta ctaaaaagta tgagattgcc gatatctgga taggtgtgct atactacaga
    11941 aaaagatata cgatctgcca ccgggtagta aaaaagagga tgatcattcc attaggtctt
    12001 tgaagggatg attctcctct ttttgtgtat tttttatatt gaaggaggaa cgtatgtatg
    12061 gcatggatgg aattaatcat agcaggaatt ttggaagtat tctggtcaac gatgatgaaa
    12121 tggagtgatg gtttttctaa aatcaattat tctttttaca cagtgattgg aatgattgct
    12181 agtttttact ttttgtcaaa ggcaatcaaa agtttgccta tgagtctctc ttatcctatt
    12241 tggacaggga ttggcgcagt tggttcggtc attattggtg tggtcttgtt tcatgataag
    12301 ctgaatcttt ccacttggtt ctttgtcggg ttgctgctca tcagcatcat tggcatcaaa
    12361 atcactagcg gtcactagat acttagacaa gcagacaagg actgatcaga aaaagcgggg
    12421 ctttttattt gatattcctc tttctatgaa aaaacaagaa aataagacta atgacctagc
    12481 gacttctcta ctcatcaggt gtatgcttgt actatctata gaaaaggagt gaatattttt
    12541 gaaaagtctt tcggtccaag aattatccaa gaactttggt cacaaacaag ccctcagcga
    12601 tgtcagtttt agcttatctc ctggcaaaat cgttggatta gtaggtccta atggcgctgg
    12661 aaaaacaacg atcatgaaag ctattttagg actgatccat tactcagaag gaacgatccg
    12721 tattggtgaa aatgagattt ctccctctag tcataaagga ttagaacaag taggagcatt
    12781 gatcgaatat ccgggtatct atccattctt aacaggttat gatcatctga agttattctc
    12841 tgaaacaaac gatgtatctg ctatcgacac tatcgtgaaa cagctcaaga tggaaaaata
    12901 cattcacaaa aaagcgaaat cctactcgct gggaatgaaa caaaaattgg gaattgctct
    12961 tgccttactg aaccatccgt ctatcgttat tttagatgag ccgatgaacg gtttggatcc
    13021 acaagcaaca agagatgtgc gaaaaatcat taccgacttg gctactcagg gcacctcttt
    13081 cttgatctcc agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc tgatcctgat
    13141 caatcaagga aaaatcatca aacaatgtac aatggctgaa ttgaaccaaa cagcgcaaga
    13201 tttcatcgtt atctctacga caaatgatca agctgctcga acgatcttga cagacgctgg
    13261 atatcccttg gaagatcctg ataaaatcaa gcttctaaaa aaaaacgaag agttattagg
    13321 aactgttatc cagttattgt ctgcagaaaa tgtccatgtg acagatgtcc agcatctgac
    13381 gaatgatttg gaatcttccc tattggaaat tctggattca gtaaaggggg aagaagaaaa
    13441 atgacgttgc ttataaaaca agaattattt aaattgatca agaaaaagtc aactgctgtt
    13501 ttatctgttc tattagttgc tttgctcata ggaacagcac ttctagccaa aaaatacacg
    13561 acgatcattg atccagttga aatgactgct cagcttttca gtgccacatc ttggattgtc
    13621 tttatcatga ttgctgctgc aagtacgatc atttcaatgg aatcacaata tggtacattg
    13681 aaaaatctcc tttatcgaaa gtattctcga ggagaaatat tagtaagtaa atggatcaca
    13741 ttagttattt attctattta tttatacctt ttagctatta ttgtcaccgt gctgatgaaa
    13801 ctgatccttt tcccttcgat ttcctttacg gagaagattt ctactggtca gacactgatc
    13861 caaagcttat tcacttatac tttgggtagt tatatcggtt tatggctaat tttaagttta
    13921 gtgttgatga ttgcttgttt tatcaatagt tccggtgcct ccatctcagc agggatcgtc
    13981 ttttattttg cttcatcaat catatctggt atcctcatcg cactcattca aaaatgggag
    14041 tggatcaaat ggaatccaat cagtatgctg aatctgcaaa accaaattgg caacgaagag
    14101 attatgaagc aattgactca tctttctact aatcaaatgc tgtttggcaa tttagcttac
    14161 attgctttgt tcttagcatt gggatacctc gtattcaagc gaaaaaatgt atgatggttc
    14221 attaacaaag aagttgtgac agaagtggcc agtttcaaga aatttcggct tatttttaga
    14281 agaagctact tcttgaacac catttattca aaaaaagaag cgatgacacg aatgttgtca
    14341 ccgcttcttt tttgaataaa ccacaggctt cacagcctct ctttatactt ctatacgtga
    14401 agcatcccag tggcaaataa aaccattgcg attactccga ccatcgtcac accgataagt
    14461 aaaatcttat ctaagtggat atgttgtaca ggttcgtttt gttctttacg tgctttgagg
    14521 taaaaataaa tacctggtaa gaaaagaaca agaacaagca tcacttcatt aaaggctaca
    14581 actagcatgg cccatacttg aaaagcactt gctaaaaacc ctactaaaaa ttggacccac
    14641 tctttccgct gataagaaag cttcatttgg tacagcccta agaaaagcca cgtgaagaaa
    14701 atcgctgctg taccaagtgt ataagcaaag ttatatgcac tgtcagtgaa taataatgaa
    14761 agtaaaaaga cattcgtaca taacgtcgtc aatacgatag cataagttgg tgccccattt
    14821 ttgttgagtt ttccccatgc tttaggcaaa agtccgtcct cagccatatt ccccattgtt
    14881 tcaatcggca agattgtcca tgagagccaa accccaacta aagaaatgat caaaccgccg
    14941 ttgatcacga ccgcccccca tttaccaacc atcatttcaa atacatatcc catagcaggt
    15001 tgcgctttga tcgtttgcag ctgattcatc gtcattgtgc catatggaag catggaaacc
    15061 aagacgtaaa taatgatcaa accgaatatt cctagaatcg ttgctttccc aacatccgat
    15121 tgtttttttg ctcgtgaacc tacgacagaa gctccttcaa taccaacgaa tacccagagc
    15181 atcaccatga tgcaactcaa tacttgacta gaaaacggga tcggctgccc attcgcattc
    15241 aatatgatat cgctagaaaa gttattaatg aattgttgga tcgaaaaggt gctgatagc
//
LOCUS       DACEDO010000066        15285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEDO010000066 DACEDO010000000
VERSION     DACEDO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.066"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(42..408)
                     /gene="ssrA"
                     /locus_tag="IWO52_002194"
     tmRNA           complement(42..408)
                     /gene="ssrA"
                     /locus_tag="IWO52_002194"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(579..1352)
                     /locus_tag="IWO52_002195"
     CDS             complement(579..1352)
                     /locus_tag="IWO52_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0356445.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1496..1846
                     /locus_tag="IWO52_002196"
     CDS             1496..1846
                     /locus_tag="IWO52_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0356446.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1827..2543
                     /locus_tag="IWO52_002197"
     CDS             1827..2543
                     /locus_tag="IWO52_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0356447.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2543..3991
                     /locus_tag="IWO52_002198"
     CDS             2543..3991
                     /locus_tag="IWO52_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0356448.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4061..4570)
                     /locus_tag="IWO52_002199"
     CDS             complement(4061..4570)
                     /locus_tag="IWO52_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356449.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4560..5285)
                     /locus_tag="IWO52_002200"
     CDS             complement(4560..5285)
                     /locus_tag="IWO52_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356450.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5508..7628)
                     /locus_tag="IWO52_002201"
     CDS             complement(5508..7628)
                     /locus_tag="IWO52_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0356451.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7858..9036
                     /locus_tag="IWO52_002202"
     CDS             7858..9036
                     /locus_tag="IWO52_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0356452.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9052..9810
                     /gene="aroD"
                     /locus_tag="IWO52_002203"
     CDS             9052..9810
                     /gene="aroD"
                     /locus_tag="IWO52_002203"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0356453.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9833..10318
                     /gene="ybaK"
                     /locus_tag="IWO52_002204"
     CDS             9833..10318
                     /gene="ybaK"
                     /locus_tag="IWO52_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0356454.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10389..11642)
                     /locus_tag="IWO52_002205"
     CDS             complement(10389..11642)
                     /locus_tag="IWO52_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0356455.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11759..13108
                     /gene="gdhA"
                     /locus_tag="IWO52_002206"
     CDS             11759..13108
                     /gene="gdhA"
                     /locus_tag="IWO52_002206"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0356456.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13220..14566
                     /locus_tag="IWO52_002207"
     CDS             13220..14566
                     /locus_tag="IWO52_002207"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0356457.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14874..15260)
                     /locus_tag="IWO52_002208"
     CDS             complement(14874..15260)
                     /locus_tag="IWO52_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0356458.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4989 a         2737 c         3104 g         4455 t
ORIGIN      
        1 aataaggatt taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt
       61 cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa
      121 ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct
      181 ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga
      241 gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga
      301 gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac
      361 gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa
      421 gggttacgtg tatttttcat acaaaattag tatagcatat attgggataa attcaataaa
      481 aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc
      541 gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc
      601 aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt
      661 aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa
      721 tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc
      781 agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga
      841 ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga
      901 gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga
      961 acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag
     1021 attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt
     1081 ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg
     1141 aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt
     1201 tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc
     1261 atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga
     1321 tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa
     1381 gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat
     1441 tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat
     1501 ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc
     1561 aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca
     1621 ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc
     1681 attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct
     1741 tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata
     1801 tgacctggaa actggaggga acgataatga atattttaat gattgaagat aatcaatctg
     1861 tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg
     1921 atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat atgattacac
     1981 tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt
     2041 caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg
     2101 gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg
     2161 ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag
     2221 tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag
     2281 aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt
     2341 ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct
     2401 gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac
     2461 aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat
     2521 tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg
     2581 aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac
     2641 gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac
     2701 gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt
     2761 gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa
     2821 cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca
     2881 atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg
     2941 acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg
     3001 agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact
     3061 gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt
     3121 tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa
     3181 tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga
     3241 tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga
     3301 aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac
     3361 aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa
     3421 cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt
     3481 ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac
     3541 agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac
     3601 attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca
     3661 aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat
     3721 tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat
     3781 gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa
     3841 aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt
     3901 gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg
     3961 tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc
     4021 gatcttctat accgcttgtt tttactataa aatgattgat ttagcccgcg ggctctaaac
     4081 gatcaatata aggactttga gcaatagtag catcatgagt aaagagtaag ataccttgat
     4141 tttcttcatg agctaaataa gaaagcaagt ggagtagctc ttggatctcc aatacactaa
     4201 gttcatcaaa tgtatcttca ataacaatat tgtttcgctt ggaaagcaag gcatgaagta
     4261 attgcagctt tactttttca aaagacgtca actcagctgc cgcacaatta agcaaggaag
     4321 gagagatatc aagtttttct tgataggcca aacgatcttg tttgatttct ttttccggta
     4381 catccaacag gacatggtct ttaagattca gataaggaaa catcggccaa tttttttgga
     4441 tgatcgcaaa attatccagt tttgcaacaa gaacctcttt tttcaagtgc tccaatattt
     4501 tatgctgttt ttcgagagga cacaaaataa cggtcaagtg ttcagcatga atcaaatatt
     4561 caggtttcat tacgtccagg cctcctactt tgcaaatgat ggataaaaaa agtgaaaggg
     4621 aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta
     4681 agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc
     4741 atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta
     4801 gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt
     4861 acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa
     4921 ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat caattcctgc
     4981 ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat
     5041 aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct
     5101 gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat
     5161 tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa
     5221 caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt
     5281 ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc
     5341 ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa
     5401 gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga
     5461 ggagcagcga caataatttt gtcgctgctc cttttaaatt tattttatca ttcggaagaa
     5521 cttgtggtcg ttccgtgaag ttcttggtac gtttgcgttt tataaagatc aacagttgat
     5581 tgtccgttgt gttcactaaa gacactagtt gacttgtcac caagagattt ttcagcttct
     5641 tttaatttct ctaagccatt cttgtagtca taatcactgg tatcaattgg gtctaatcca
     5701 ctattcgtat agaaccgaag aagatcacca ttattgattt ggtcagaaac agctaactgt
     5761 ttatcaactt tttctttcag tgaatcaatt tcattctgta cttcctcagt aggttcagta
     5821 atagcttcac cagtttcgtt actatagatc gtaccaccgt agtaagtata ttttggtgta
     5881 acaaaatcac catcacggaa agaaacaagc tgttcatgat ctttcgagaa tagatcttgt
     5941 cctaactgaa tataattttt tgtatctaca cctagtaagt gaagtaatgt aggcaaggca
     6001 tcaacttgtc cgccgtaagt gtgattgatt ccgccctgtg tttgtccagg gatatggatc
     6061 atataaggga cacgctgcat ttgtgcattg tcgtagtttg tccaagtaga acttgttttt
     6121 ccaactaatt cagctagatt tttatttctt gaattcgaga caccgtaatg gtcaccgtaa
     6181 aggacgatga ctgaattatc atacaagccg ctttctttca aataattgaa gaattcttgt
     6241 acggcggtat ctaaataatt ggctgtcgca aagtaaccgt tgatcgtttc atcatttgta
     6301 ttggctaacg ggaatcctgc ttcgtcattg gtgaattttg aataaggata atggttagat
     6361 acagcaatga attttgaata aaatggttgt tgcagacgtt ccaagtattg tacggattgt
     6421 tggaaaaatg gtttatcatg caacccgtat tggaacgaat tgtcatcatt aacatcataa
     6481 taagaagaat caaagaaata atcatatcct aaattcttgt atgtctcgtt tcgattccag
     6541 aatgtaccag cgttcccatg gaataccgca ctaatgtaac cttgtgtttg acttaaaata
     6601 tcaggtgcag cttggaaagt attttttccg cctaattgtg tgaacaagga gccttgattc
     6661 aagccaaaca atgaattttc catcagcgtt tccgcatcgc ttgtttttcc tgcttttact
     6721 tgatggaaaa agttgtcaaa actgaacgtg ctgttgctgt ggtaaagact attgatgaaa
     6781 ggtgtaactt catgctggag accattctca tctgtcaatt tataatcgat caggaattgc
     6841 tggaagcttt ctaaatggat ataaatcaca ttttttcctc tagcgagccc gaacaaatcg
     6901 tcatttggtg ctgcataatg ttcttttaca taattttcta cttctttcat atcattggga
     6961 cttgcttctg cccgcacttg gtttgtctgg taagtttgaa caccgtcata aaccatgaaa
     7021 gcattcaagc caagatattt gaccaaataa tcacgagaga atgttcgagt cagcaattct
     7081 ggacgatcag cttccgccat aaaaagattt cctgagaaaa tcataactgc taatgtagag
     7141 atcgcaaaag ccattcttgc tcggatcggg cgagggtcca acttgatttt ttttgcaatc
     7201 agcagaacag tcaagataat aaaatcgatc caatagatca catcatatgg gcgaaacagt
     7261 cgaatggcac tttctcccag accactcgct actttcccag cacctaaaat cgtgttgatc
     7321 gtaatgaagt ccgtgaattc tcgataataa gtgacattcg aaaaaacaag caatgtcaaa
     7381 agaaaataaa tgaccatcat cgtgatataa gcaactctag ggcgtctgat ataaagagca
     7441 attccaagta ataacaatgt agttgcaata gggttgataa acaagataaa gtattgaatg
     7501 ccactttcga ttccgagatg gaaatcaact aaataggcaa aaatcgtttt caaccaaagt
     7561 aaaacgacta gtaaagaaaa aaatccgagc ctcttattca aaaaggcagg tgtttttata
     7621 catttcaaga taatcttcct ttctgcactt cttaatcaca gaagtgtcct gaaaccatgt
     7681 tacacagctc acttattctc atttatttta ctagtatgac aatatagtgt caattgaaac
     7741 cagaagttta tgctgtttca ttaaggtttt ctttaagctt catgaaaaaa tgagcagtaa
     7801 gacaagtcct atgttttttg ttatacttgg gaagagaaag ctaaagcagg tgaaggaatg
     7861 aaagtaaaag taaaaaaaag agcaagcaat cgattgaaaa aaggatatcc actgatccaa
     7921 aaagaagacc ttgctcatcc ccaaaaagaa accacgcaat gggtggaatt agttgattcg
     7981 caaaaccaat ttatcgcaaa aggatatcta ggaaagcaga ataagggaat cggttgggta
     8041 ttgagccagc agaacatccc atttgatcaa tctttttttg aagaattttt tgtgaagtca
     8101 aaagaaaaaa gaactttctt tttttcagat gattctacta ccgcttttcg tatattcaat
     8161 ggagaaggag acgggatcgg cggtctgatt gttgattatt atgcaggtta tgctgttttt
     8221 tcttggtata acgaaacgtt atatgcacat cgtgaattat tgattcaagc ttttcgtgaa
     8281 atctacccgg aaatcatagg aggctatgag aaaattcgat ttgaatcagt aaatctgcct
     8341 gagagccagt tcatttacgg aaaagaggca ccggaaccct tgatcgtaaa agaaaacgga
     8401 gtaaaatttg ccgtttattt aaatgaagga ttaatgactg ggatcttttt agaccaaaaa
     8461 gaagtacggg gatcattagc agaaggtttc gcttctggga aaacagtttt gaacatgttt
     8521 agttataccg gcgccttttc tgttgctgcc gcaatgggag gggcaatcga aacaacaagt
     8581 gtcgatctag caaaacgcag tttaaaaaaa acacaggaac agtttgaagt gaatggtttg
     8641 gatgtcgatc agcaaaaaat cattgttatg gacgtattcg agtatttcaa atacgccaaa
     8701 agaaaacaat ttttatatga catgattgta ttggatccac caagttttgc cagaaataag
     8761 aaaaaagtct ttcgcgtagc aaaagattat ggggaactgg tcaaggatag tctggatatt
     8821 ttagcagaag acgggatact tattgcgtct acaaatgctg cgaatgtctc gatagaacaa
     8881 ttccaaacaa tgattgaaga ggaattccat gccaaaaatg tttcttttga acaacttgct
     8941 ttatatcgtc tgccaaaaga tttccagacg acggatacct ttttagaagg caattatctg
     9001 aaagtgtttg tttatcaaat taaaaataac cattagaagg agcgatgcag aatggttcgg
     9061 gtaaagggat tgaatatagg aagcggtaga ccaaagattt gtgcgcctct tatcggggcc
     9121 gtccgagagg aagtattacg tgaagcagtt cttgcacgtg aagcaggggc cgatttagtc
     9181 gaatggcgag tcgatcatta tcgtgaagtt ttttcttttg atgaagtagc agaaacattg
     9241 gttcttatcc atgagatgct tgatggactg ccgttattat ttacgtttcg taccttagct
     9301 gaaggcggag aacaagacat atcgatccgt cagtatcgcg acctttatga acatgtgata
     9361 cacacaggat tagtagatat ggtcgatatc gagttgttca aagtagaaag tctaggaaaa
     9421 agactgatag aggaaatcaa gtctttaaac attcctttaa ttatcagtag tcatgatttt
     9481 aaagaaacac cagcagaccc tgtcttgctt taccgattga atgttatgga acattttgga
     9541 gccgatatcg gaaaactggc agtaactccc aataatgaac gagatgtttt gcggttgatg
     9601 gagttgacta gacgggcaaa tgcttttttc tccatgccta ttattacgat gtccatggga
     9661 aatcttggta aaatttctcg tctggttggc ggaacgacgg gttccgttat gacatttggt
     9721 tccttaagca gcgattctac ttctgctcca ggacaattac cggtcaaagc attgaagcaa
     9781 attattcaat tattaaatga accgttgtag aacaagtttg aggtgatatt ttttggcaaa
     9841 gaaaaaaata gtaaaaacga atgcaatccg tatggtcgaa caaaaaaaga ttccctatac
     9901 agagcacgaa tatgagtggg acgaaagcca cctaagtgcg tcatcggtcg ctgaacagct
     9961 tccagaaagc caatctcgta tatttaaaac actggttgct gtgggcaatg tgacggggcc
    10021 tcttgttgct gtgataccgg gagaagccga attgaatcta aaaaaattag caaaagtttc
    10081 agggaataaa aaagtagaaa tgctccattt aaaagatttg gaagcaacga ccggatacat
    10141 acgtggtggc tgctcgccta taggtatgaa aaaactattt cctacttatt tagatcaaat
    10201 tgcagaaagc tatgaacaaa tcattgtctc ggctggcaga agagggctac agatggagtt
    10261 agcacctcaa gatatctgtg cattaacgag cggacaattt gcagatataa aacaataaat
    10321 catacgtata aaagaaagaa ggaggccggg atgttgtttg tccagtctcc ttctttcttt
    10381 tttatacatt atgctctttg cccggcattc attcctgcta catgccctgt agcgaaagcc
    10441 gctgtgatat taaagccgcc agtataacca ttgacatcca gtaattctcc cgcaaaatac
    10501 agaccatcaa tgattttact ctccaatgtt ttaggataaa tttcctttag agaaacccct
    10561 ccgccagtta caaatgattt ctcaatagga aaagttttgt tgattgatag cttaaactct
    10621 ttgcatagtt gaacaaaatc ttgaatttgt ttttgagagg cttgttgtcc tgtcagatga
    10681 tccatttcaa gccgctcaag aaagaaaact aacaatcgct caggcaatat accatgccaa
    10741 gcattgacca agttcttttt ggttgcttta cttttttctg tcaatatatg aataagctct
    10801 tgtttggttt gtgtagggaa acaatccaga gaaagtgtca ctggttctcc cgttttctct
    10861 aattctttat tgacaaaact tgaacaacgc agtgcagccg gaccagaaat accgaaatgg
    10921 gtgaaaagta aatccatcgt atgttcagtc aatacacgac cttttttatt taaaacgcgt
    10981 aaagtgatgt cttgtaaaga aagcccttga agtgttttct cctgaatata cggttcatcc
    11041 gatatcaagg gggactctgt cgcataaaga ggcgtcaccg tgtgacccac tcgcttcacg
    11101 atcttgtacc catctccagt agaaccagta gatggataag tccgtccacc agttgttaag
    11161 ataatggcag gagcatggaa ctcttcgaat tccgtgcgta taccgtaaat tttatgttct
    11221 ttgtggatca atttctctac tcgagtagag aatagaagcg tcacatctag ttctttcaga
    11281 cgattgagca atgcttcgat aatcgttttt gatttatttg ttacaggaaa catccgtcca
    11341 tgatcctctt cttttaaatg tacgccttgt gattcaaaga agttcatgat atcgaaatta
    11401 ttccattgcg caaacgtact atataaaaac tttccatttc caggaatatg tgcaatcaaa
    11461 tcatcaacag gacgattgtt cgtaacattg caccgaccac cacctgtcat cagtagtttt
    11521 tttcctgctt ttttgttttt ttcgatcaag agtacgcgtg cgccttgttc agctgcactg
    11581 atcgctgcca tcatcccgct tgttccagcg ccgactacga tcacatcata cagtttttcc
    11641 attccctcac ctcaaggaaa gtgtagcata tctgaagaaa gaaaacagaa aaaaatgttt
    11701 tgagccaaaa gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat
    11761 gacaaacgca acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg
    11821 gcaagaagaa tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga
    11881 cactcatccc gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt
    11941 tattcaattt cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta
    12001 ccgtatccaa tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag
    12061 tgtcaatcta agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac
    12121 cggtctgcca atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga
    12181 taatgagatc atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc
    12241 atcgattgat gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt
    12301 cggcgaatat aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga
    12361 tttttgggga agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa
    12421 acatctattg aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag
    12481 cgggaatgta gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac
    12541 atgttcagat tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa
    12601 agaagtcaaa gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc
    12661 tatttatcat gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc
    12721 atgtgctact caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat
    12781 caaaatcgta gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca
    12841 tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc
    12901 tgctttagaa atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga
    12961 gcagctggat caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta
    13021 cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa
    13081 agcaatggca gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca
    13141 ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa
    13201 cctataagga ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt
    13261 tgtcaacgaa caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt
    13321 acgggaaggt actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta
    13381 tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga
    13441 agtattagtt gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt
    13501 cttgaatcac acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt
    13561 ctttgccgga aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga
    13621 tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc
    13681 gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg
    13741 tatctacgct acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg
    13801 ttgggaaact ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt
    13861 tggtttgtta ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc
    13921 tgatgcaagc aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc
    13981 agcaatgcgc aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga
    14041 accaggaatg caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa
    14101 agaccaaaaa ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg
    14161 acaattcatc caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc
    14221 tcgtaaatca atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca
    14281 aggaaaagaa gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac
    14341 agacggtgac gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg
    14401 ttatacaatg tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa
    14461 cccattcgac caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa
    14521 accaggcttt gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc
    14581 gctagattta aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca
    14641 tttaggtact caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt
    14701 gtcacagtcc cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag
    14761 gactttaaga acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat
    14821 cagtaagcaa aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat
    14881 aggcctaatt tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct
    14941 atgatccgct tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca
    15001 ttggcagagt caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca
    15061 gcataagcat ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg
    15121 atatcagttt ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa
    15181 gtagactgat agtagtccca acctttccat cctataaaag caatcaatat gataacgatc
    15241 aaccctaaaa tttttttcat gaaagatctc cttttttatt tttat
//
LOCUS       DACEDO010000067        15217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEDO010000067 DACEDO010000000
VERSION     DACEDO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.067"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(24..722)
                     /locus_tag="IWO52_002209"
     CDS             complement(24..722)
                     /locus_tag="IWO52_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0356459.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IWO52_002210"
     CDS             complement(715..2067)
                     /locus_tag="IWO52_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0356460.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IWO52_002211"
     CDS             complement(2290..3690)
                     /locus_tag="IWO52_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0356461.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IWO52_002212"
     CDS             3851..4588
                     /locus_tag="IWO52_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0356462.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IWO52_002213"
     CDS             4713..5192
                     /locus_tag="IWO52_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0356463.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IWO52_002214"
     CDS             5189..5956
                     /locus_tag="IWO52_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356464.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IWO52_002215"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IWO52_002215"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0356465.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IWO52_002216"
     CDS             complement(6859..7461)
                     /locus_tag="IWO52_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0356466.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IWO52_002217"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IWO52_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IWO52_002218"
     CDS             8142..8726
                     /locus_tag="IWO52_002218"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0356467.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IWO52_002219"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IWO52_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0356468.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IWO52_002220"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IWO52_002220"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0356469.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IWO52_002221"
     CDS             10627..10806
                     /locus_tag="IWO52_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356470.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IWO52_002222"
     CDS             10828..11853
                     /locus_tag="IWO52_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0356471.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IWO52_002223"
     CDS             12028..13272
                     /locus_tag="IWO52_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0356472.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IWO52_002224"
     CDS             13425..14372
                     /locus_tag="IWO52_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0356473.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IWO52_002225"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IWO52_002225"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0356474.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
BASE COUNT         5188 a         2720 c         3111 g         4198 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gcttttt
//
LOCUS       DACEDO010000068        14727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEDO010000068 DACEDO010000000
VERSION     DACEDO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.068"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(537..899)
                     /locus_tag="IWO52_002226"
     CDS             complement(537..899)
                     /locus_tag="IWO52_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356475.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(973..1353)
                     /locus_tag="IWO52_002227"
     CDS             complement(973..1353)
                     /locus_tag="IWO52_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0356476.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1435..2610
                     /locus_tag="IWO52_002228"
     CDS             1435..2610
                     /locus_tag="IWO52_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0356477.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2686..3270)
                     /locus_tag="IWO52_002229"
     CDS             complement(2686..3270)
                     /locus_tag="IWO52_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0356478.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3471..4469
                     /locus_tag="IWO52_002230"
     CDS             3471..4469
                     /locus_tag="IWO52_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0356479.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4581..5090
                     /locus_tag="IWO52_002231"
     CDS             4581..5090
                     /locus_tag="IWO52_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0356480.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5120..5779)
                     /locus_tag="IWO52_002232"
     CDS             complement(5120..5779)
                     /locus_tag="IWO52_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0356481.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5781..6545)
                     /locus_tag="IWO52_002233"
     CDS             complement(5781..6545)
                     /locus_tag="IWO52_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0356482.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6553..7230)
                     /locus_tag="IWO52_002234"
     CDS             complement(6553..7230)
                     /locus_tag="IWO52_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0356483.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7267..8652)
                     /locus_tag="IWO52_002235"
     CDS             complement(7267..8652)
                     /locus_tag="IWO52_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0356484.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8862..8946)
                     /gene="ffs"
                     /locus_tag="IWO52_002236"
     ncRNA           complement(8862..8946)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWO52_002236"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8969..9484)
                     /gene="tadA"
                     /locus_tag="IWO52_002237"
     CDS             complement(8969..9484)
                     /gene="tadA"
                     /locus_tag="IWO52_002237"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0356485.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9591..10448
                     /locus_tag="IWO52_002238"
     CDS             9591..10448
                     /locus_tag="IWO52_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0356486.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10529..10864)
                     /locus_tag="IWO52_002239"
     CDS             complement(10529..10864)
                     /locus_tag="IWO52_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356487.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10931..12301)
                     /locus_tag="IWO52_002240"
     CDS             complement(10931..12301)
                     /locus_tag="IWO52_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0356488.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12379..12915)
                     /locus_tag="IWO52_002241"
     CDS             complement(12379..12915)
                     /locus_tag="IWO52_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0356489.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13224..14705
                     /locus_tag="IWO52_002242"
     CDS             13224..14705
                     /locus_tag="IWO52_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0356490.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4525 a         2923 c         2391 g         4888 t
ORIGIN      
        1 cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca agcgattgtc
       61 aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc aactcattta
      121 tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc gatagaatca
      181 agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt tagtaacatg
      241 tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg
      301 ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga
      361 aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa
      421 ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac
      481 aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag
      541 aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct
      601 tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca
      661 aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg
      721 ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt
      781 ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat
      841 tttttagtac catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa
      901 atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa
      961 aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat
     1021 ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa
     1081 ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa
     1141 actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac
     1201 ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag
     1261 tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt
     1321 tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat
     1381 tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc
     1441 gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct
     1501 gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct
     1561 cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc
     1621 actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg
     1681 gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca
     1741 gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca
     1801 ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa
     1861 aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta
     1921 aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa
     1981 ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc
     2041 catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat
     2101 ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc
     2161 aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta
     2221 gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat
     2281 gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca
     2341 gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact
     2401 tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact
     2461 cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat
     2521 gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga
     2581 atcaagtatg ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct
     2641 tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc
     2701 aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc
     2761 gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc
     2821 tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc
     2881 ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct
     2941 tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc
     3001 acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc
     3061 agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc
     3121 ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg
     3181 gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc
     3241 actttttaaa tctagttgtg ggaatgccat attgtttcct cctaatattt gcatacttca
     3301 taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt ttcttttatt
     3361 tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa acatttttga
     3421 gaagcattct caattaaatt ctgctatagt gtgaaaaggg aggaattttt atggaaatct
     3481 atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa
     3541 tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt caattagcaa
     3601 ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata aaagcaagtg
     3661 aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc catttaaaag
     3721 aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca aataaaggca
     3781 tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag ccaagaaaat
     3841 tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc gtgaatgatt
     3901 tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct gctatcgatt
     3961 ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt cgcccggaat
     4021 ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac ttgcttactc
     4081 cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc cggttaaaaa
     4141 aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc aattatggat
     4201 tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga aatgccatta
     4261 ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt gacatctgta
     4321 cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct actgcagtga
     4381 acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac agcacaagct
     4441 tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt tcaagttaaa
     4501 cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag agaaagaaaa
     4561 aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa aaggcaagag
     4621 aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc atgtttttac
     4681 aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc
     4741 taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat atattagcag
     4801 aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa ggcctttacg
     4861 gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc ggctttacga
     4921 attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac agccacgatg
     4981 gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct gctgctgcca
     5041 gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa
     5101 aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc aagctatttt
     5161 ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat aagataaagc
     5221 tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg ggatcgaata
     5281 accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta
     5341 tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac ggacgaatca
     5401 accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc gggatcaaag
     5461 tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa aagtgatggg
     5521 ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag ggattattga
     5581 gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa gaagtataat
     5641 ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg atcgtcacac
     5701 caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat tccatccact
     5761 gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt cacataggta
     5821 gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa aacgagcaaa
     5881 gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc tccaaccatg
     5941 attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat gatggcttct
     6001 ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga gatgactgac
     6061 cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt cgttccaatg
     6121 aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata ggtcagataa
     6181 ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa tccggcagct
     6241 aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc ttttccatct
     6301 gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg agctacatga
     6361 atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt cgtgttattg
     6421 gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg tttaccggcc
     6481 ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata tcctttatag
     6541 tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg ttttttctac
     6601 ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact ctgtttttcg
     6661 atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct catcgatata
     6721 cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc
     6781 gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc
     6841 caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt
     6901 taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg ctagtacatc
     6961 actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg acacaagccg
     7021 ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta gctcgccttt
     7081 gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca gttcttcggt
     7141 gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt tatgctcaac
     7201 gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt ttattatacc
     7261 ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac ttcggataaa
     7321 ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa atggaacaaa
     7381 catcaaatga tctacggtag taaaagtata atttttattc agatcgattg gcttgttctt
     7441 ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca tctgcccaaa
     7501 aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt tacgattttt
     7561 ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat ttaacaagtg
     7621 catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt ttggaatttc
     7681 agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc
     7741 ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca gagaaaatgg
     7801 caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat aactatttat
     7861 ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt tggctgaatg
     7921 cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat actgcccgta
     7981 ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga ataaatgatg
     8041 ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt ggaaatcatc
     8101 ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta aatcttcgac
     8161 taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat tataagtaag
     8221 cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat
     8281 tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag
     8341 gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac
     8401 gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt
     8461 ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc
     8521 tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa
     8581 ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac
     8641 tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt
     8701 cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt
     8761 acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat
     8821 aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc
     8881 ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc
     8941 ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta
     9001 atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt
     9061 cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat
     9121 tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta
     9181 cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg
     9241 cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt
     9301 ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt
     9361 ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg
     9421 cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta
     9481 ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc
     9541 tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg
     9601 ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg
     9661 atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc
     9721 aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc
     9781 catggtatcg ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga
     9841 tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt
     9901 tggaattatt tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag
     9961 tcgaacaaat aaaccgtgat tatcttgatg gtactgaaat caacggtatt tttctatctt
    10021 tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca
    10081 ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc
    10141 tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag
    10201 atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa
    10261 tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa
    10321 cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg
    10381 gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac
    10441 ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga
    10501 atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc
    10561 ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt
    10621 ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac
    10681 catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta
    10741 ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg
    10801 ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt
    10861 tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga
    10921 actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta
    10981 cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc
    11041 catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca
    11101 agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc
    11161 gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag
    11221 aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag
    11281 cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt
    11341 gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag
    11401 tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt
    11461 tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag
    11521 gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt
    11581 cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta
    11641 ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc
    11701 actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa
    11761 ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg
    11821 tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt
    11881 tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg
    11941 ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag
    12001 cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga
    12061 ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta
    12121 attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc
    12181 gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc
    12241 ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca
    12301 ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct
    12361 actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc
    12421 cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc
    12481 atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt
    12541 gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc
    12601 taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa
    12661 taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata
    12721 aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata
    12781 aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat
    12841 tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt
    12901 tactctcccc atcattctta cctccgtctc ttttcatcta cccataaata aataacataa
    12961 ataattctaa aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat
    13021 caatttttcc gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata
    13081 gaataaacaa ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg
    13141 tgagaataac acagactgaa atgactttcc actcacagtt tttgtctaac tatatacaaa
    13201 aacagaataa aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc
    13261 tacaaatggt cattcaaaaa aataattgtt ctttacaaga attatccaat gacttacata
    13321 ttccaaaacg gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc aagcaatttt
    13381 tattgattga tgaatttatt tattctaatc ataaaggaga gatccaaacc aaagaaaccg
    13441 atcaaactaa aaagttagct gttttttcta agttaaaatt atgctaccta aaagaatcca
    13501 atcgttttaa ctatttgatg ttactaatca attatccaca aactgctgta ccaaaaaaat
    13561 atttacttga acaactttat atatcgccct cctatctcga aaaactgaca aaacagctca
    13621 atcattcctt aaaaaaattc catttacgga tcctatctgt aaaaaattgc tgtactcttg
    13681 aaggaaatga gttatctatc cgtctctatc tttacttttt tctttcagat acatttggag
    13741 gtaacgattg gccattttcc tcacttgaat taaatgcact aaaaatgaac agtacagatg
    13801 agcaaataga aaaaatttca aaacaagaaa aaaatatcta cttgctgtta actttattca
    13861 tattacgaaa caaacaacag gcatattttc caaaaacttc ttctgaaatc actgaattga
    13921 tggacttatt gcagcataca catgattatt cagaatactt tacttctttt tcagtgagtc
    13981 gcccttgtga agagacagaa acagagagag cctactttaa ttattttttc tattgttttt
    14041 ttccaaatca aatctctttt cgacaaaaac aggagattgg ttttttattt tccacaagca
    14101 aaaaccttat ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa cagtttcctc
    14161 tcaaccattt agaaaataaa ataccaattt atttttattt tcttgtgttg gcagttgttt
    14221 ttttcgaact gttgggtgaa tccagcagta gtttttctac actttgcttt ccagaagcca
    14281 cgtccaatca ttctagttct tttatacagg aaaagaaatt aagagaaaaa atcgatacaa
    14341 ttttttcaaa aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac
    14401 acttactaca aacagaaacg aaagagcagt tactcatcca tttgcagata accaaacagt
    14461 ttacaggaaa ttatcatctt caaagccatt tgcagcagtt atttcgcaaa gatgctgtta
    14521 aaattaccgc atattcagaa catgctgatt tgatcattac agatcagcat gagaaaattt
    14581 cagatgaaac ggagactttc tatctggata caacgcacaa tacagtgtta tttggcaagt
    14641 taataaaagc ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa acaaagagga
    14701 tgtgacaaaa ttcttgtcac atcctct
//
LOCUS       DACEDO010000069        14421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEDO010000069 DACEDO010000000
VERSION     DACEDO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.069"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1150
                     /locus_tag="IWO52_002243"
     CDS             join(<1..264,264..1150)
                     /locus_tag="IWO52_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0356491.1"
                     /translation="RRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKWVKQAQ
                     TTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNKHKYSI
                     SAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLANLGLQV
                     SQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIVTDLTY
                     VRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFHTDRGK
                     EFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLANLRLE
                     LFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            1430..2410
                     /locus_tag="IWO52_002244"
     CDS             1430..2410
                     /locus_tag="IWO52_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0356492.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            2532..3950
                     /locus_tag="IWO52_002245"
     CDS             2532..3950
                     /locus_tag="IWO52_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0356493.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4021..5490
                     /locus_tag="IWO52_002246"
     CDS             4021..5490
                     /locus_tag="IWO52_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0356494.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            5500..5967
                     /locus_tag="IWO52_002247"
     CDS             5500..5967
                     /locus_tag="IWO52_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0356495.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6003..6875
                     /locus_tag="IWO52_002248"
     CDS             6003..6875
                     /locus_tag="IWO52_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0356496.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            6957..9491
                     /locus_tag="IWO52_002249"
     CDS             6957..9491
                     /locus_tag="IWO52_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0356497.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            9647..10420
                     /locus_tag="IWO52_002250"
     CDS             9647..10420
                     /locus_tag="IWO52_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0356498.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            10413..11204
                     /locus_tag="IWO52_002251"
     CDS             10413..11204
                     /locus_tag="IWO52_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0356499.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            11214..11705
                     /locus_tag="IWO52_002252"
     CDS             11214..11705
                     /locus_tag="IWO52_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0356500.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            11723..12133
                     /locus_tag="IWO52_002253"
     CDS             11723..12133
                     /locus_tag="IWO52_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0356501.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            12137..13537
                     /locus_tag="IWO52_002254"
     CDS             12137..13537
                     /locus_tag="IWO52_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0356502.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            13560..13934
                     /locus_tag="IWO52_002255"
     CDS             13560..13934
                     /locus_tag="IWO52_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0356503.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         4972 a         2038 c         2918 g         4493 t
ORIGIN      
        1 aacgtagaac ctattcaaaa gaattcaaac aacaagtcgt cgatctttat ctcgcaggaa
       61 agccccgagc tgaaatcatt cgagattatg agctgactac ctcggccttt gataagtggg
      121 tcaagcaagc gcaaactact ggctcgtttc aggaaaaaga taacttgacc cctgaacaag
      181 tagaactaat tgcattacga aagaaaaata aacagcttga aatggagaat gacattttaa
      241 agcaagcggc gctgatattg ggacgaaaag acaagtaatt gataccaata agcacaaata
      301 ctcaatatca gcgatgtgta aagtgttaaa catctcttgc caaacctatt attatcaagc
      361 caaaccagtt gaagatgaat cagaactgga agaactcgtt caagaagaat ttattcgcaa
      421 tcgaaaagct tatggtacca ggaagctgaa gcagtgcttg gccaatctag gtcttcaagt
      481 gagtcaacgc agaatcggcc gtatgatgaa acagcgtggc ttgcgttcaa catacacatt
      541 ggctcatttc aaattccatt cttctacttg taacgaagcc aagaccgcca atattctaga
      601 cggaaccttc ctacaggaaa agtcattaga agcgattgta accgacctta cctatgttcg
      661 tgttgggaag aaatggcagt atatctgcct gattcttgat ttgtttaatc gagaaatcat
      721 cggctattca tgcggggaga agaaagatgc gacgttagtc aaggaagctt ttgcaaagat
      781 tccgtactcg ttgaataatg tacaactttt tcacacggat cgtggaaaag aattcgataa
      841 ccagacgatt gacgaaattt tatgggcctt taatatcacg cgttccttaa gcaaaaaagg
      901 ctgcccctat gataacgccg ttgcagaatc cacctataaa tctgtcaaag tagaattcgt
      961 tcatcaatac cgctttgaga cattggctaa tttgcgatta gagctatttg attacgtcca
     1021 ttggtggaat accatccggc tgcacggcac gttaggctat gagacaccga tcagttttcg
     1081 acagcagaga ttggcgcagc gaatccttga taatgagctc ggatgtgata ccggtggcga
     1141 ggcaccttaa ttatttgaat gagcttctgc cttagagaat cagattcgag gaggctcgtt
     1201 gtcaagggca gtcggaacac cagcagcaag cttacgcaat accttataat ttttgtcaaa
     1261 aaaagtgttg ccattccaca tatatcttta aacagctatt gccagataaa aataaatgat
     1321 attatatata taaatactta tccgaaaagg ggaatgagag agtcaaggtt cttggaatgt
     1381 cgaatagtcg agtaggtaga ttttctatct acaaccttgt tgtttaaaaa tgaaaaaaat
     1441 tggaattaga gaagttgcga aagaggcaaa tgtttctcct acgactgtgt cgagggtatt
     1501 gaatgatcgt ggatatttga gtgaggaaac aaaaagaaaa gtttttgatg ccatgaaaaa
     1561 attagaatac tatcctaatg aactggcgag agcacttttt aaaaacgaaa catataccgt
     1621 aggtttgatt tttcctacaa ttactaatcc atttcacgct gagttaattc aagatatcga
     1681 gttcgagctt tccagcaggg gatataaagt gttgctgtgc aatagcttaa acaatccaac
     1741 aaaagaaaaa gagtacttga cgatgttgag aaagaatcag gttgatggaa ttatagtagg
     1801 aacgcacaat aaagatattg cggattatga tattccaggg ctacctattg tagcgattga
     1861 tagaagtctt ggtgaaaata cagttacagt atcttgtgat aattacaggg gtggggaatt
     1921 agctactcaa ctacttgtaa atcatggctg tcagaatata ctgtgtattc ggggagatag
     1981 taagataaag ctacccgcga atagtaggac tttagcatat aaagatgttt tgaataaatt
     2041 catgctggaa ccgttagtcc tagaagtacc atttgtaaaa gaaatcaatg aaaaaaatga
     2101 aataattaag acatatctgg aaactcatcc aaatattgac ggggtttttg caggagatga
     2161 tttattggct tctttggcga ttaacaactt aggaagttcg ggcaaaagag ttccaaaaga
     2221 ggttaaagtg attggatttg atggagctca acaaacattg atttataatc cagaattatc
     2281 aactattcaa cagcctatag atcaaatatc taaagtagca gtaaaaaaat tattgaatat
     2341 gattaagggc gaaaaggaga cggatgaact ttcacttcca gtaaagctca taaaaaaaaa
     2401 tacagcataa gttaaatcgg cgaaaaattc cttcgtcgat ttaaaaatat ttttatgaaa
     2461 cgggttgaca tatgaaacgg gttgacatat attagttgtg aagataaaaa ccttttcact
     2521 ggaggaagaa aatggagaaa ttattaatta aaagcaataa tcatcaactt tactaccaac
     2581 ctaaggatgt ttgggtcgga gatgttatgc cttacgggaa agaaggaaaa ttctttattt
     2641 atcatcaacg tgatactaga aatccagttc cttttggaga accgtttggc tgggctttag
     2701 caactacgaa agattttgtt cattttgaag actttgggga atctctttta agaggggatg
     2761 acaatgagag agaccagttt atctacgctg gttctgtatt tgaggcaaat ggaaaggttc
     2821 acgcatttta tacagggtat aatcgtgaat tcttagcgca gggaaagaca tctcaagtgt
     2881 tgcttcatgc tgagagtgat gattttgttc attgggaaaa atcagaaaca gcattggagt
     2941 tagaaccaca agaagggtat gatattcgaa actggagaga tccatttgtc gtttgggatg
     3001 atgaaaaaga agaatatttg ctaattttag gggcgcgaaa gggtcaggat aaacatctac
     3061 aaaccggacg tttagtggaa tttacatcaa aagatttaac taattgggaa ttcaaaggtg
     3121 atttttgggc tccgggattg tataccatgt ttgaaatgcc agaacttttc aagattggtg
     3181 actggtggta tcttgtctat tcagaatact cagatggaaa taaaatactc tatcgaatga
     3241 gtcaaagttt ggaaggtcct tggattaagc ctaaagatga agcttttgat ggacgggctt
     3301 attatgcagg tagaacagct tatgatggaa aacgtcgagt cctatttgga tgggtaccaa
     3361 caaaagatca agaaaatgat aaaaacaatt atttatgggg aggtactttg gttcctcatg
     3421 aagtctatca aaaagaggat ttttcacttg gggtaaaacc catagacgaa gtgtggaatg
     3481 cattcataaa tttacatgat attgccgatg tagagataga aacagcagac acaagagaag
     3541 aagtaacgct tgtcaaaaat agtggaactt tgtttaattt agaaacaaca atttcatttg
     3601 atgagaatct tagtgacttt tcactgagat tatataaaaa ccttgagaca gatgaatctt
     3661 acgaatatcg cttcaattta gatgaggggc gtcttacatt tgataaaaac ccttgttacc
     3721 cttggtttca atatatggat aaaggactta ttagacctat atctttaaag cctaaccaat
     3781 cttataaact aaaagtgatt gttgatgaca atataatgac actttatatt gatggaatcg
     3841 ctcttaacgc tcgtgtacat ggttgttttg gtcaagcaat tactattaca gcaacaaatg
     3901 gatcaatgac tgcaataaat accaagtttt caaatgaaat aaagaaataa taaacgtcaa
     3961 tcatgtggca ggtctgaaaa tcaaacatga cctgtcacat ttaaaaggga ggataaatga
     4021 atggactaca aaaaaattgc cgaagatgta ttggaaaacg ttggaggaaa agagaatatc
     4081 tcaagcgcta cacactgcat tactcgttta cgtttaattc ttaaagacaa aagcaaagcg
     4141 aatatggaaa tgcttgagga aatagaaggt gcaaaaggtg ttcttttcaa tagtggacag
     4201 ttacaagtaa ttttcggaac aggtataatt gaaaaggttt acaaagagtt cgttgaattg
     4261 acaggagcta aggaagtatc cgtttctgaa atgaaagacg aaggagtcaa taattcagga
     4321 aatagacttc aacgagcttt taaagttttc tcggatatct ttattccgat cattccagct
     4381 tttattgggg cagcaatggt tttaggaatt cgttcgttga ttaccactcc tggaatgttt
     4441 ggaatggatg gctcattcgc tgatcacaat gtattcctaa atgatttatc caaatttctg
     4501 ggtattattg caaccacatt tgattattta ccagttttag ttatgtattc tgcaacaaaa
     4561 cgttttggcg gaaaccctat tttaggatta cttgtaggat ttgttatgat tcatccagga
     4621 ctagccaatc ggaatgattt cgtcatggga actataaagc ctgattattg gcatttattt
     4681 ggattaagca ttccagcagt tgcgtttcaa gggggtgttt ttccagcaat tttgacttct
     4741 tggtttttag caaaagttga gaaaattaca aataaatatt ctcctcaagt acttgcttac
     4801 attttagtcc caactgtgac aattgttctt gccaactttg cactattttt agtgtttggt
     4861 ccattgggga attttatcgg aaatgtttta ggtggaatca ttgatttcct atatatgaag
     4921 atgggtgcct ttggtgcttt tgtttttgca ggcgcgttac aaccattagt tgtaacagga
     4981 acacaacatg ctattcaagg tattgaggca aacttagtcg caacaactgg attcaattat
     5041 atccaaccta tttggtcagt ttcaattatt gcccaaggtg gcggttgtat tggtatgtat
     5101 ttactcttta agaaaaaatc taaagataga gatattgcga tttcaagctt tattccaaca
     5161 ctggtaggta ttactgaacc ggctatcttt gctgttaatt tgaaatattc aatcgttcct
     5221 tttgtttgct cgtttattgg tgctggtttt ggtggagcat ttatgaaaat ttttgatgta
     5281 aaagccattg gacaaggatt gactgtattg ccaggtctga ctattgctaa tccagttctt
     5341 ccttatatat ttggtaatct catagcattc attttaccaa tcatttttat tctcgcttat
     5401 gataaatata aagtgtttat ccctgaagct aaacaccgta aatttgcaga aaaaactgac
     5461 tcaaaaaaat tgccagttca agtaaagtga gatgagagca tgttaaattt ttttagaaaa
     5521 aatgaaataa gcgttgtagc acctgcaacc ggtgtttttg aaaaactgga gaaattaagt
     5581 gatccagtct tttcaaaagg aatgatggga caaggatttg caattgatgc aaaaaacgaa
     5641 acgattgtat ctccaatcga tggttttgtt tcaagtattt ttcctacaaa acatgcctta
     5701 ggaataaaaa caaaatcagg aattgagata cttgttcatg taggaataga tacagttgag
     5761 ctaaatggtg agggatttga tattaaagtt caagaaggac aaaaagttaa ggctggggat
     5821 attcttatga ccattaattt tgtagttttt gaaaagaatc acaagactaa agatgtcatt
     5881 gttgtttttc cagactacca agacgaaatt ttcattccga aacttgaaga agtaaaacaa
     5941 ggacaagaat tgtttgttct tggctaatga taagtaatta ataatagaat cgaggaaaat
     6001 gtatggtttt tggggctatt gaagctggtg gaacaaaatt tgtttgtgcc attggagata
     6061 aagatttgaa catatttgaa aaagtcagct ttcccacgac tacacccaaa gaaacaatga
     6121 atttagtttt agattttttt aatcatcata aagatgattt agaaggtatc ggagtgggat
     6181 cgttcggtcc tattgatatt cattgtgaat ctaaaacgta tggcttcatt acatcaacgc
     6241 caaaacttgc ttggcaggat tttgatttta ttggaagttt aaaaaagcaa cttaatattc
     6301 caactgcttg gacgactgat gtaaatgcct cttgctatgg tgaatatgtt tctggaaatg
     6361 gcagaggcct ttcaagtgtt gtttactata ccgtaggaac gggcatagga ggtggcgcta
     6421 tccaaaacgg aaacttcatt gaaggtttta gtcatccaga aatgggacat atgcttgtaa
     6481 aacgacacaa taaagacaac ttttctggga catgtccatt ccacggagac tgtttagagg
     6541 gcttagctgc tggtccggca atcgaaagaa gatcagggaa aaagggacaa gaagtgactg
     6601 aaaatgacgt tacttgggaa atagaagctt attacattgc tcagtgtgca tataatacaa
     6661 cattgatgtt atcacctgat gtcattatat ttggtggcgg agtaatgaaa caaaaacact
     6721 taaaaaagaa agtacaagat aagtttattg agttgatcaa cggatatgtc gctattcccg
     6781 atgttgacaa ttatattatg acaccaaaat tgttagataa ttctggtatt gtaggttgtt
     6841 tagctttagc aagagacgtt aaagaaaaat cttaatttgg aaaaaagatc gtctcgacta
     6901 tttgtttggt tgatgcgatc ttttttttca attagtaatt gaagggacgc ctaaaagtga
     6961 aaaaaaaaat gaaagatgaa gtattagagt atatcaaaaa taatttaaag tattacgatt
     7021 tttctgctca agatattgca atgaaatttt gtattaaaag aaatgtcgct tcccactatt
     7081 tgaatcagtt attttcagat ggaaaattac taaagaatga ttctgttaga ccagtaatgt
     7141 tcaagtataa tcaacaaaag ccaaaagact gcttttctaa gtttattgga gcagacatat
     7201 ctttgaaatc cactatagat aaatgcaaag ctactgtaat gtatccccct aatggcctcc
     7261 cattaataat aaaaggaaac agtggcgttg gaaaaagttt tttagcaagt ttgatctatc
     7321 agtatgctct tgatagaaaa gtaattcata atgacgcaaa atttgttgtt gtgaattgtg
     7381 cagattatgc aaataatcca gagctattgt cagcagtttt atttggatat aaaaaaggag
     7441 cctttactgg ggctgaaaaa gatactgcag ggatgctatc ttctgctaat ggaggatacc
     7501 tttttttaga tgaggttcac aatctaagtg cagaaaacca agagaaattg tttcttctta
     7561 tggattctca aaagtatcgt aaacttggtg aaagtgtgca atgggagtat gcaaatgttc
     7621 gccttatctt agccacaaca gaagataaaa atacctcttt gttagcaact tttagaagac
     7681 gcatcccagc tgaaatcact ctgccagatt acaataatag aagtacatct gaaaaaattc
     7741 aattattatt tgaatttctt aaagatgaag caattacaat tgattcaaaa atattttgtt
     7801 cgattgaact ttttactgat cttttatcga aaacatttga aggaaatgtt ggagaattga
     7861 ggaatgaaat caagcttctt tgtgcagaag gttatttagg aaataagaga aagtcttcaa
     7921 tttatttagg ggacaaacta gacagcggtt tttggataga tccagaaatt agtatagatt
     7981 taaaaaaaat gtttcttaaa tcactttctg aaattgattt gattgaactt tttcaaaatc
     8041 acatttcaat tgaagaaagc atgagtcaat taagagatag aatacttaaa gagtgtgcag
     8101 gttcttctga gtataattat ttagaaagtg acgaatttat agcattaaga aactatgttg
     8161 ttaataaaat atcaccaatt attgattcga caggtctatg tttattagat gaattcttgg
     8221 cggatatcag tctatttata atgtttattg atgtaattga agaaacatca agaaattttt
     8281 tgctgtctaa gttcagaaaa tttcgtatga aaaatgataa acaaaaatta ttggcaaatg
     8341 agatttggag tagtttagaa gtcgaagaaa gtaaacaaga acttttgttg aaatggatac
     8401 tttttcttgt aaagaagttc tatgtgaaaa taccagaaac ccattgtctg atagttatgc
     8461 acggtaaaat aacagcttct gcgatcgcaa gggaaacaaa taagttgctg aatacatatg
     8521 tttatgaggc tttcgatatg ccaattgaag gtgagactag tgacttaatt aacctaatca
     8581 acaatttttg taaaagtata gatacgacga atggactcat tctgctagtt gatatgggat
     8641 cattggaaca aatgtatgaa aaaattgaat ccaatgtaat aggtgatttg gtaattttaa
     8701 acaatgtttc tacagcttta gcgattgaat gtgggattca agtttgccaa aaaaagccaa
     8761 ttagtcattt ttaccagatg gattttgatt cgtttcaagt taaagttcag tattataaag
     8821 gattatcaca aaagaaaaat gtaatagtat cttgtctctc tggtgagggg atttctgaaa
     8881 aagttaagga tattttaaaa cggtatctcg ataatcaagt ggagattttg atttttgatt
     8941 ttaacgcatt aaagaaaata gcaaaggaaa aagatactat gatttttaag aatacaattt
     9001 gtgttctttc aaccactgaa ttttacattc aaggaatcga ttgtttaaat ttagaaaatc
     9061 taattaatgg gaatcaaacc ttagaaaaat taaataagta tatggactcg gatagctgtg
     9121 agttctgctt aaatgaatta gttaaactct ttactattga aggcgctagt actaagcttc
     9181 gctttttaga ttccaaaaaa gtattcaatg agatagaaaa agtactatat ttgtatgaaa
     9241 aatattacga tgtcaaaatc ccaagttttc tgagaattaa tctatttcta catctttctg
     9301 gaatgattga acgaattatg attggtgatg gaataacaaa tgaacatttg aaagagggag
     9361 cgcaactatt tgatactttt ttaaatgtat caaaagattt tttttcagaa atcaaagata
     9421 tttactgtat tgaaataccc aaaggagagt atgagttaat ctatacaatt tttgagcaga
     9481 ctattttcta aatagcatac tagcgtctaa aaaaatgaaa gggtgtccaa agtatattta
     9541 gacgctcatt taattaatat ttactgtatt ttcccgcttt attggataat gttagttggc
     9601 acagtaattg cttatataaa agtataagaa gaaaggagag aacaaaatgg cgttttggca
     9661 aacattttta gttgtgttgt atggattttt cattaactgg gataaggaag ggcccaaaat
     9721 cggaacctcc caacctgtcg ttgcaggttt cttgaccgga cttattatgg gagatattca
     9781 aactggactt tatatcggtg ggactttgca attaatgaca ctaggtatta ctagttttgg
     9841 tggtgcatca gtaccggatt atcaaacagc agcattgatc ggtacttatt tagcaatctc
     9901 aactaaccag gatgcttcta taggaataac tttagcaata ccggtagcaa tgttaattgt
     9961 acaactagat gttctaaagt ggtcaacaaa catttttttt caacaaaagg cagaaaaatt
    10021 cgcacaaaaa gaagattatc aaaaattaga gtggatgcaa tatttggcat caattaatac
    10081 aagctttgta tctggaattc ctgttttgtt aactgtgata ttaggcccaa cgttagtggg
    10141 aagtgcaatc aattatattc ctgaatgggt taccggtggt ttaaaggtag cagggggact
    10201 attacctgct gttgggatag gaatgctact taaatattta cctacaaaag attactttgc
    10261 ctatttgtta gttggtttcg ttttggcagt ttatttccaa ataccaattt tagggatttc
    10321 gttacttggc gctgcaatcg cactaatcgt atataaaaat agatcaaata atactcaagt
    10381 agcaccagct gcaacaggag gttttgacga agatgaataa gaaactgaca aaaatcgaca
    10441 aacaagtaat tttccggtgg attctcaatg gtgcaacttc attaaattat gaaaaaatgg
    10501 aagggttggg ttatgcgtat tctatgcttc cttttatcaa ggacacatat aaagatgatc
    10561 ctgaaggaat gaaacaatcc gttttagctc acctacaatt ttttaacact acgccgtata
    10621 cagcgcctta cattattggt atgaatgttg gaattgaaaa agaagaaaaa aataagtctt
    10681 tggaagcagt aagtgctctg aaaacaggtc taatgggtcc tttagcggga attggagata
    10741 gtgtatttgt agttattcca tggactattt ttggagcaat tgcagctaat atggcattgg
    10801 aaggtagttt tgttggaatt attttgtgga tactggcatc aattttatta aggattactg
    10861 cttatccttt ataccttgct gggaaagaat cgggtgcgag cctactaact caaattgaaa
    10921 caaaattaaa gtctattact gatgcagtat caattttagg gttaatggtg gtcggtgcac
    10981 tcattgccac agttgtaaaa gtgaatgttg gctttgaatt taaacaaggc gacttaacaa
    11041 tgaccggaaa tgaaatcttt gatcaagtta tgcctggttt attacctgca tgtgtagtag
    11101 caattgtgta ttatttgtta ggacgaaagg ttaaaccagt ttatgtaatt ctattaatct
    11161 tagtagtttc tattgcgtta agagcgttaa atattttgaa gtaggtgttg aatatggatg
    11221 aaaaaatgaa aggtattata catgttcgta ttgatgatcg aatgattcat ggacaagtag
    11281 caacacaatg gacaaatcaa ttgagtgcta acagaattat ggttattaat aatgaagtag
    11341 ccaatgatga cgtaaaaaaa gctgtagttc gcctcgctgc accacctaat gttagaactt
    11401 caataattac aagggaagta gcagttaaaa atattctttc tggtaaatat gaaggacaaa
    11461 aagttcttat tgttgcagtt tctccaatcg atgttaaatt tttgattgat aacggtttac
    11521 caataacaag tgtaaacgta ggaaatttat caagaaggaa tggaacagaa cgcatccgtc
    11581 cgacgataaa tattactgaa gaagagagag aggcttttaa agaactaatt agtgatggag
    11641 ttgaagtgac agtgatacaa actcctagag attcaaaaga atttttaaag gattttattt
    11701 attaagaaca gaggatgagt ggatggagtt tataattaca tctcatggaa atattgccat
    11761 tggatttgta gatacattaa aaatgttttt tggcgataat ttaaattgtc atgtggtaac
    11821 acttggagat gcaggcattg aagaattccg tgaaaacatt gaaaaaatag tttctcaagt
    11881 aaaaaatcaa gaagttatgg tatttgctga cttgattggt ggaacacctt tcaacgaatt
    11941 tgctaaaaga agtgaagtat ttcaaaatgg gtttcaactt cttggtggca tgaatttagg
    12001 aattttgatt gaggctgtca atttgagatt acaaggaaaa aaaagcgatg atatcatcga
    12061 aaatctaaga aatatgaata caattgcgtg tcttgcagat atgcaaagac atactaacga
    12121 agaggatgag tgataaatgc aaaaaatttt ttacaagcct aaagattttt ggtttgggga
    12181 ttgcatgcct ttttataaag atggaacttt ttatctattt catcaacgag atacgagaaa
    12241 tcctaaacct ttaactgatc cttttgggtg gtcactagtt acaaccactg attttgtgaa
    12301 atttgaagaa tacggggaag taattcatcg tggtggcaac gatgacattg atcaatacat
    12361 ttacgctgga agtattgtcc aaggaatcaa tgagtattta gctttgtata ctggacataa
    12421 tcgcaaagct aagttggatg gaaaaacttc agaagtgtta atgattgcaa gttctaaaga
    12481 tttaattcat tggaaaaagg atggcgaagc agcaaaactt gttcctcaag aaggatatga
    12541 taagaatgat tggcgcgatc cttttgcaat ttttgatcaa gatcatggaa aatacgtttt
    12601 gatactagga gctagaaaac cagcaagtaa gaaaaagccg acggggagat tggtctattt
    12661 tgaatccgaa gatttaaaag attggaattt caaaggagac tactgggcta ataatgaatt
    12721 taacatgatt gaaatgcctg atatatttga aattgatgga acgtggtatt tacttttttc
    12781 agagtattgt gaagataaaa agactaaata tcgagtcgga gcaggattat tttcagattg
    12841 gaaatcacca gtagacgaag ctttcgacgg aaaagcctat tatgcagcaa gaacagtcgg
    12901 aactaatgaa actggccgtt ttctaactgg ctgggtggca acaaaagaag gggagaacga
    12961 tcaaaataac tgggattggg gcggtgcact tgttccacat caaattattc aaaagacaga
    13021 caaaactctt ggtgttaaac tgcctgaaaa aataattgag aattttaaag aaatcactcc
    13081 attttcaagt tttaagttag agaaagaatt tggaaaaaaa gaaagagtaa ttgttcctaa
    13141 aatcgaagat tattttctat ttgaaacaac tttgactatt acatcgttaa caaaggagtt
    13201 tgctttaaaa ttatttgtag atagtgatac tcaagaagga tacgaatttc gtatttcatt
    13261 gattaataat cagcttactt tcggaaaaac tccgaactat ccttggcctc aaatgtttga
    13321 taaggggctg caacgaccac tatttttgga aataggcaaa gaatatgaac taaagttaat
    13381 tgttgatgat acgatagctg ttttatatat tgataatgtt gctttaagta ctagaatgta
    13441 tgataaagtc ggaacagcat taagtatgac tgttacggag ggagcaatca aggtagataa
    13501 actagactat tcaataaatt atgaatgtgg attttaaaac aattgaaggg atgatgtaag
    13561 tgaaattaaa tggtttacaa catattggaa ttccaacaaa agattttgga gcttcaaaaa
    13621 tcttttatga atccttaggg tttagtctga ttaatgaaga aatgaatgtg ggaagtaaag
    13681 tcggcttttt cgagttgaat ggaacgataa tagaaatatg ggaagataaa actactgagt
    13741 gtagaggagc cattgatcat ctagcattag atacggatga catagatgag gtattcaatt
    13801 caattcaaga tttaggatat gaattattag ataaaaatat tatggaatta ccgttctggg
    13861 aaaatggaat ccgttacttt aatttctacg gaccaaatca tgagattata gagataagcc
    13921 aaatgaagaa ataaatagat aacgaagtgc tctaagttgg tatgaacgaa aagtacttca
    13981 ttttttggta tcagcatttc aatagtttaa tgagaattta ctatttcaaa ttaattcaaa
    14041 gtgacagtat tttttactta tttggtttca aattttgtaa tccttcctat ttattggaat
    14101 acactccaca ttttgtatac aagtcatatc atgttttagc aaagctctta ccaagcaaag
    14161 ttataatgat gttttccaaa gtttgatctt tcgatggagg ctaacacata ttttttgcag
    14221 gacagccaaa attagatgta tagaatagag tctgtgaggc gtatagacag tttattgtct
    14281 gtatgccttt ttttattttg aaaaattttt agtatttttc gaaaaatgct cactcctaga
    14341 gtaagcgccc catagaacag taccttacta acaaatgcac ctcaagatat acttgagcta
    14401 tcaagaagtc tggaggtttt c
//
LOCUS       DACEDO010000070        13783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEDO010000070 DACEDO010000000
VERSION     DACEDO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.070"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(67..723)
                     /locus_tag="IWO52_002256"
     CDS             complement(67..723)
                     /locus_tag="IWO52_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0356504.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            916..1194
                     /locus_tag="IWO52_002257"
     CDS             916..1194
                     /locus_tag="IWO52_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356505.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1213..2421
                     /locus_tag="IWO52_002258"
     CDS             1213..2421
                     /locus_tag="IWO52_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0356506.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2569..2934)
                     /locus_tag="IWO52_002259"
     CDS             complement(2569..2934)
                     /locus_tag="IWO52_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0356507.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3081..3854)
                     /locus_tag="IWO52_002260"
     CDS             complement(3081..3854)
                     /locus_tag="IWO52_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0356508.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4028..4888)
                     /locus_tag="IWO52_002261"
     CDS             complement(4028..4888)
                     /locus_tag="IWO52_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0356509.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4888..6306)
                     /locus_tag="IWO52_002262"
     CDS             complement(4888..6306)
                     /locus_tag="IWO52_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0356510.1"
                     /translation="MKIVGVTACPTGIAHTYMSVEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6363..6812)
                     /locus_tag="IWO52_002263"
     CDS             complement(6363..6812)
                     /locus_tag="IWO52_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0356511.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7257..7520)
                     /locus_tag="IWO52_002264"
     CDS             complement(7257..7520)
                     /locus_tag="IWO52_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0356512.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7619..8914)
                     /locus_tag="IWO52_002265"
     CDS             complement(7619..8914)
                     /locus_tag="IWO52_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0356513.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8911..10182)
                     /locus_tag="IWO52_002266"
     CDS             complement(8911..10182)
                     /locus_tag="IWO52_002266"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0356514.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10347..11216)
                     /locus_tag="IWO52_002267"
     CDS             complement(10347..11216)
                     /locus_tag="IWO52_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0356515.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11508..13118)
                     /locus_tag="IWO52_002268"
     CDS             complement(11508..13118)
                     /locus_tag="IWO52_002268"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0356516.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3923 a         2902 c         2346 g         4612 t
ORIGIN      
        1 aactattttt ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt
       61 tgttcatcaa aagataatat tgtcttctaa caagtccata ttcaaaataa cgatatgatg
      121 gtctttaacg gtgataatac catctttttt cagttgtttt aaaattctgt ttacactgct
      181 agcagttgag atgccacaaa atttggcgat ttcttcattc gtgatcgaaa aatcgatcag
      241 catcccttct tctgtccgca caccgaataa atcatataat tcgtaaagtt gcgtacaaac
      301 agctcccagc tttccgttta atagcatttg ctgcattttt ttcatcgaat aaagcagacg
      361 atagcggtaa tactctttta catagtgttg tagctttggg tcttggttga tattttgcca
      421 aaagcgcaca cgattgattc gataaaactc tgcatattct gattcaactc gaatattata
      481 cggcgaatcg atgaattgtg aatattcgtc ttttaaaaga gagacgattt caagatcttt
      541 gatatagcga aggttaaatt ctctcccatc atttgaaata acacttgctt ttaccgttcc
      601 ctctttcaag atatacgtat gggaatcttc catcccttcg aatgtcaaat atttatgata
      661 tcctttgcgt ataatcggaa aatcattttt atccaaatat tcctttaaga ctctactatc
      721 caatgatgct cccccatcca aactttcatc taaaaagatc atacagtact ttaaaacttt
      781 ttaaagtcca aaggtatttt actaatgata ttaacatttg ttaagtacct ttttccatca
      841 cttatgcgat ttttttcctt ttttttattc gttatactgc agaagtaaaa ttttttaaga
      901 atagagggaa aaaacatgaa ttactggatg aacacaatca ttaatcgatt agaaacagca
      961 tatcaaacaa gatttgatat gaaagcttct ttagtctttt taaatgatgc gtatcaaaac
     1021 tcgatcgaat tgatcaaagc agtggatgag aatccaacaa acgaatgcga agaattttta
     1081 aatctgttta tgtctacaag agatttattt attcgccaac ttgttgatcg ctatccatcc
     1141 aattatcacg atgtagaagt tcaaatacaa aaattaaaag catattcagc ttaaggggaa
     1201 tgtataacat acatggaaaa gaaattattt aataaatatt ttattggaat cacattgatc
     1261 aattttattg tctacctgat ttattatctg ttgatggtca tcatggcagt cattgcgcaa
     1321 gacaccttac acgccagctt gagccaagca ggttttgctt ctgggatcta tatcatcgga
     1381 acattgctgg cacgcttatt catggggaaa cagttagaac tatttggtcg gaagtttacc
     1441 ttaagaggcg gcgcgatctt ctatctcctt tcaacattag catatcttta tacgccaact
     1501 atcggggcat tgtatttagt tcgattcttg aatggattcg gctacggtac agtttctaca
     1561 gctacaaatg ctatcgttac tgcatatatc cctgcatcta aaaaagggga aggcatcaat
     1621 tattatggat taagtactag tttagctgcc gctgttgggc catttttagg aatgattttg
     1681 ctgaatatga ctgattttcg tttcattatc tggttttcaa ttgtactggt cttctttgtt
     1741 acgattgcct gccttgtctt tcctgtaaaa aatattccat tatctgaaga acaaaaaata
     1801 tctcttcgct catggaatat caatagtttt attgaaaaga aagcactttt cattacagga
     1861 attgcctttt tgatgggtct ttcttattca agtgttttat catttttatc ttcttataca
     1921 aaagtaatcc acttagttgc tgctgggtca tttttcttcg tcgtatatgc tttggtcatc
     1981 acatttacac gtccattgac tggaaggatt ttcgatgtca aaggagaaca atacgtgatg
     2041 tatcctagct atttattctt aacagcagga ttatttttac tgagtgtaac gacgaatagt
     2101 gtgacattgc ttatctctgg agcattagtc ggcctaggtt atggtacgtt tatgtcaaat
     2161 ggacaggctg tttgtttaaa aatcgtccgc gaacatcgaa tcagtattgc tttatctacc
     2221 tactttattg gtttagacct aggcttaggc gttggtcctt atttgatggg gatgctaaaa
     2281 tctcttactt catttagagg attgtatgtg attgccggag tcattccatt gatatgtaca
     2341 gcactctatc ttgttaatgg ccgaaaacat agtcatgaag cagagggcga attgattgaa
     2401 tcattgaaaa atgaaatttg atcttgatga atagataaaa aaaaccgttc agatctgaaa
     2461 ataatctgag cggttttttg agtaaaaata gtttatgatt attcttaatt aaacatctat
     2521 ttatttttaa agctttcatt tatcgataag aatagtttaa ctgttgattt attccatttt
     2581 ccgttcatct ggtacgatgc cttgacttag caaccacttg tggaagaacc attctgccac
     2641 accgatcaat agagcaggaa tcaatacttt catccaaata tcgactgtat cattcataaa
     2701 atatagatag agccacaaga taactgcagc tgaaccagca tctgcgatcg tcgctgcgat
     2761 gttccctact ttacgtaaga tcaacagatc ccctagcaca tagatcatga ctacaataat
     2821 taccccaaca atcgttgaat ccataaagct aacaccgtat aaacctgtca gcacgaccca
     2881 cagaatcgcc cagatcatga ttgccttcac taccaacgcc ttcacatgtt tcattttctg
     2941 cactcctttc tcctggataa aaaagtaaga ttatctaaca agataacctt ttcattatta
     3001 taccatcggc tgagagaagc gagcaaagat aaggtcctat ctaaaaatga ggaagccact
     3061 tagaacattc acgtaataat ttatccgtac agacattcga tatggtgttc ctttactttc
     3121 gcatactgtt cttttgtcag ttctttactt gtcaccagat aatcgatttc atgaaaagca
     3181 atggcttgat gcatgaattt atgtgcaatt ttttgctgtg taactaaaac ggcaagccgg
     3241 tctgattgtt ttctaaaagc ttttttgacc attgcctctc cttcatgagt ttctgttact
     3301 ccttggctga aatctagccc gcgacaactc attatcgcta aatccgcata atatgacaat
     3361 gcatcgttat aggcttttga accgttgatt gttgctcgtt tcggaacaat tgcccctccc
     3421 aatacagata cttttgccgt tgtatattca ctcaacatag cagctgtgac gatgccattc
     3481 gtaatgatct gcaaaccatc aaatcttgct aggtttctga ctaagtgtaa agaggttgta
     3541 ctggaatcca agaataagac catatcatct cgaatcaaag aagaagctgt atctgcaatc
     3601 ttacgttttt ctgtttcttc ttctaatttg gtgatcttca tcaggtaagg cacgtcaatt
     3661 tttcgattgt caataagcat ggcgccaccg cgaactctac ggatcaatcc acttttttcc
     3721 agaaaaacaa ggtcacgttt aatcgtggct tcgctataat ttactaactc gatcaaatcg
     3781 gtgactcttg caaattggtt gttttttaga taattcacaa tctcctgctg tctattctcc
     3841 acttcattca acataaaatc accccattta ctttatatag taacaggaaa aaagaggttg
     3901 tgacagaagt gattggcttc gagaaataag aagaaatttt tcgaaaaaac tacttctgaa
     3961 catcgtgtgt ggttaagagg gtatgacaag actattgtca taccctcttg accacacatt
     4021 tttattttta gtataaacta gccttgccta ctgtgttcaa aatttccatt ttatgtttga
     4081 ccacttcttg taccttttta ttagctgctg gataaattgc attcggctcg tacatcgatg
     4141 gattttcact caatatttct ctcaaagcat tgaaaaagac gcttttaaga tctgagctca
     4201 aattcacttt ggcaactccg tattttactg cttcgctgac ttctgcgtcc ggattacctg
     4261 aaccgccatg taaaacaaat ggaagatcga tttgttggtt caattctttc agtaagtcga
     4321 tatttaattt tggagttctg tttttcggat acaagccatg cgctgttcca attgctacag
     4381 caagtgtatc gattcctgtt tctttcacaa aaatcttcgc ttgatcagga tctgtataaa
     4441 tgattgtttc agcacctcct tcagcagaac cattattccc aatcgttcca agctcagcct
     4501 ccactgagac atttaccgca tgagctaacg caaccacttt tttcgtaatc tgtacattgt
     4561 cgtcgaatga tttcaaagac gcatcgatca tcacggaaga atagccgttt cgaatcgccc
     4621 gtaagacatc ttctatcgta ccgccatgat ccaagtggat aacgatcggc agtttacttg
     4681 tataagcata ggctcttact gccgcaacaa actcatcact taaataggcg atttcatcag
     4741 gatgaatttc taagattact ggtgcttcta atctctctgc ttcttcgatg attgctttca
     4801 tcatatcata gctgcaaata ttgaaggctg gtactgcaaa ttgattctct ttagccactt
     4861 tcaataactc tttcattgta tataacatta cgcttccacc tcatttgtta gattttcatt
     4921 tttcgtccac ttgtcttgtt ttttctcttt cactgttttt ttcaatagtc caagcataaa
     4981 ggctgtagtg actgtacctg atgcaattgc caagacatac ataggtaaat tgttcataac
     5041 atttggaatt agcatcaccc aaatccctcc atgaggtgcc attgaagtaa ctcctactgc
     5101 catggaaatc cctgcagcga ctgctgaacc tgtcatcaaa ctaggtatca cgcggatcgg
     5161 gtcagatacc gcaaacggta tagcaccttc tgtaatatac gaagcgccta aaatccagca
     5221 tgattgacct gaagtccgtt cttctttggt aaatttatgt ttgaataata aagtggctaa
     5281 agcaatacct aggggaggtg tcattcctgc agccatacaa gcagcaatcg gtgcatttac
     5341 tccagctgac atcaaaccga tcgagaaagt cgaagtcgat ttattgatcg gcccacccat
     5401 atcagctgcc atcatgatcc cgataagtaa tccgaacaat agcccggatg tatttcccaa
     5461 gctattcagc caatttgtca aagcatccaa cacccatgtg atcggttgtt caatcacaaa
     5521 taccatcaaa agcccaataa ttaagatcga taaaaagggg acgacaatca gctgataaat
     5581 agcagaaaat tcttttttta cgctaatttt atttgccaga aaatcacaga tataccctgc
     5641 tagcacgcca ccaatcattc cgccaagaaa acctgaacca cctgtagagg ccagtaatcc
     5701 ggcaacaatt cctggggcaa aacctgcttt tccagcaatc gatgtagcaa tccccgcgcc
     5761 taacgcagga accatcatgg caaacgctgt atctcctccg atccttgaaa atgcagctgc
     5821 taacacatta tagtctgctg agttgggatc agccgctgta atcccaaagg caaaactcag
     5881 tgcgatgatg attccgcctg cgatcacaaa tggaagcatg taattcacac cattcatgaa
     5941 atgattatag attgtcggct ttttctcatt tgcagctggt tctttttcat tagacacaac
     6001 gaatacgcca atatctctca atgcttcgtc tattactact tgcggttctt tgattgctcg
     6061 gcttgtagag acacgcttta cttttttccc ggcaaatcgg tccaattcga tttctttatc
     6121 tactgcaaga atgattgcat ctgcctcttt gatttcttgt tcagataact gattttctgt
     6181 ttttacacct tgtgtttcga attttgcttg atggtttaga tttcttgctg ttacttctaa
     6241 tttttctacc gacatataag tatgagcaat tccagtcggg caagcagtta caccaacgat
     6301 tttcatgatt attttcctcc tttttcttca ctatcgatac tcggacaaag actctactat
     6361 tttcattctt gactaatctg tctgatgata tctgctggcg tagtggcttc ttttattccc
     6421 tttctaaaat ctgcattcat caatcgtctg gagagtgtgc tgagaatctc tagatgcata
     6481 tctgctgatt tttcaggaac tacgatggca aaaatatatt caattggcat ttcatctatc
     6541 ccgctatagt ccgttaattt ggctgtatga aaaaaacaca gtgctggttt tgtcacatat
     6601 ttgctttttg catggggaat cccaaatcca tcttctattc ctgtggagat ctcctcctct
     6661 cgtttgaata gtgccttaac gactttttga acatttctta ccttatttgt ttttttcaac
     6721 acatccccta ttcgctcaaa taatgatcct tttgaatcta tcgatggatc gaaaacaatc
     6781 agttcttctg cgatcaattg atcaaacctc ataaaaaacc tctcccccac aaaaaatgtt
     6841 aacgttttct tggaactgag gtcagtatag catcgctagc tgtaccgttc aatattatga
     6901 atgaacagag gaaaaagacc catcatccgc gtgtataagt cattaaaatg aacgatctag
     6961 ctcacaaaca atcgcaagtc tataacaaaa caaaaagcct tccttgaaag gaaagcttag
     7021 cttctaattt taggtaaatg gttttttact tcgaaataac ccctcttcat ttcgttaata
     7081 taaggtgttt ttatcaagtg gataattaaa actatatact agtttttcat tgttttgtgc
     7141 attatgatcc tacgactttt tcagcatcaa acgaagtttt aaattggcaa aaaacataaa
     7201 aaaacagact atccctatca gatagtctgc taaacgttga gttatcagcg tttttattat
     7261 tccgcagcgt ttgcgtcttt gagaccgtat tttttgttga aacggtccac acgtccgtct
     7321 gcttgtgtga atttttgacg tccagtgtag aatggatgtg agtcagaagt tacttcgaca
     7381 cggatgactg ggtatgtatt accgtcttcc cattcaactg tttcttgtga agtttttgtt
     7441 gaacctgaca agaatttgaa accagttgtt gaatccatga atactactgg gtggtaatct
     7501 ggatggatgt tttctttcat gattttttcg ctcctttgcc ctgctccatt tgctgaacca
     7561 gagttgattt gtcgatttac aacattaaca tcttaacatg tttcagtcaa ggttgcaatt
     7621 atcttttcag atttctggtc ggattttttt taccaaagga gacatcttga aattctttga
     7681 aaaattgctg attgtttttt gttttacgca aaaagcggat cagttgttcc gtatattcta
     7741 atgaatctcc agtcatattt tttcgaagtt tccaaatttc ttctaattct tcacttggca
     7801 tcaataattc ttcttttcgg gtacctgatt ttttgatatc gatggctggg aagatccgac
     7861 gttcagataa ttcacgtgaa agttgtaatt ctagatttcc agttcctttg aattcttcat
     7921 agatcacgtc atccatacgg cttcctgtat cgactaaagc ggtcgctaaa atcgtcaaac
     7981 tgccaccttc ttcgatgttt cttgctgccc cgaaaaattt cttaggttta tacaatgcgg
     8041 ctgggtcaat cccgccactc aatgtacgtc cgcttggtgg aacgactaag ttataggccc
     8101 gagcaagtct agtaatacta tccatcaaga taacgacatc tcgtttatct tcgactaaac
     8161 gcattgcgcg ttccaatact aattcagaaa ctcgagtatg gttttgcggc tgcagatcaa
     8221 atgtagaaga taccacgtcg cctttcacgc ttcgttctag atccgttact tcttccggtc
     8281 gttcatcaat cagtagaacg attaattcta cttccggata gttttcggtg ataccatttg
     8341 cgatttcttt taatacagaa gttttccctg cttttggcgg tgcgacgatc aatccacgct
     8401 gaccaaaacc gattggagaa aagatatcga tcattcgggt agatagtctt tgcggtgttg
     8461 tttccagttg aagctgtctt tgcggataaa gaggtgttaa ggctggaaaa tgcggacgtt
     8521 cttttgcttc ttccggatct tttccattaa cgctttccac gtgcatcaaa ccataataac
     8581 gttccgattc tttaggtgga cgggcttttc ctgccacttt gtccccattt ctcaaactga
     8641 atcgtctgat ttgtgaggca gaaatataga tatcttctgc acttggtcca taattgattg
     8701 gccgtaaaaa accgtaacca tcttgaccaa cgatatccaa tattccttcc atataataaa
     8761 atccttgttt ctctgcttgt gcacgaatca cagctagaga taattccttt ttattcatct
     8821 tactgtaata aggaatttta aattctttgg cataactata aacttctttt aacgttttgt
     8881 tctctagttc tgccattgtc aaataatcgc tcattcagct cccacttcct ctatcatttc
     8941 aattgatgcc cctaaggctg ttagtttctc gataatatga tcataaccgc gtaagatata
     9001 atctacattg taaatcgtcg ttgtcccttc tgccaatagt ccagcaatca ctaaacaagc
     9061 acctgcccga agatcagaag ccacgacttc tgtgccgtga agtttatttg gaccattcac
     9121 gatgatcata tttccttcca cggtaatatc agcgcccatt cgtgctagtt ccggcacatg
     9181 gttcacacgt ttcgcataga tcgtatcgat gatttctgaa gtgcctgtag tcatcaatag
     9241 taacgcagta agtggctgct gcagatctgt ggcaaatcct ggataaggat aggtcatgat
     9301 gtttgcgggt tttaattctt tcgaggggta aacttcgatt tcatcttcgc tgattttcat
     9361 atggacacca atttcctgca attttgctat aaagctttcc aaatgttcaa agatcacatt
     9421 tttcactttg ataccatttc ctactgcagc tgctaatgct agataggttc ctgcttcgat
     9481 gcggtcagga atcatgaaat gccgacagcc atgtaatgtt tcgactcctt cgatacggat
     9541 cacatctgta cccgctcctc gaacttttgc ccccatatta ttcagtaaag ttgccacatc
     9601 tatgatttcc ggttctcgtg cagcgttttc aattactgtc tgtccttttg cttttacagc
     9661 agctaacatc acattgatcg ttgctccgac tgaaaccacg tccatgaaaa tccgattgcc
     9721 acgcaatcct ttattttctg tccgtaaata catggcacca tgttcatttg tcacttcagc
     9781 acctagagct tcaaatcctt tgacgtgtag gtcaatcggt ctagggccta aataacagcc
     9841 acctggcagg cctactaccg cttcgccaaa tttaccaagc agtgttccca taaaataata
     9901 agaagcacgc aaactattga tttttccgtg cggcatcgga atcgatacga cttctcgagg
     9961 atcgatttct agtatgttgt tggaaaaatg gacagttgcg cccatgatct ctaaaatttc
    10021 aattaatgaa tgaacatctt gtatgtccgg cacaccttcc aagatcactg gggaatcagc
    10081 taagattgca gctggaatca atgcgactgc actatttttt gcaccgctga tcgttacttc
    10141 accagctaaa ggtcggtttc ctttgattac gatttttttc atctgtttcc accttgttta
    10201 ttaatctttt ttcatggata tgcttgtatt ttatcataga taaaaaaaag aaacgagcat
    10261 ggtctgccgt tttaagtaaa aaagaatgga gagcgacttc aaaagtcgtt ctccattcct
    10321 atatatcgta tctgttatgt ggatgcttac gctttgttag ctgaaccgaa ccagtcgata
    10381 tgagatttag catcttcaac gattgctgct ttacctggag ctaataattt acgagggtcg
    10441 aatcctttac cttctttatc tttaccagct tcgatatatt tacgagtagc tgcagcaaat
    10501 gataattgga attcagtgtt aacatttact tttgagatac ccattgagat agctttttcg
    10561 atttgatctt gaggaatacc tgatccgcca tgtaatacta aaggcatgtc tggacctaca
    10621 gcatcagcaa ttgcttgtaa gtggtcaaaa gctaatcctt tccagttttc tggatattga
    10681 ccatggatgt taccgatacc gcaagctaag tagtcgatac ctgtagcaac catttgtttg
    10741 cattcttcaa tatcagctaa ttcgccgtta ccgatgattc cgtcttcttc tccgccgatt
    10801 gaaccaactt cacattcaac tgagatacct tttgcatgtg ctttttcaac aacgtcttta
    10861 gctaatttca agttttcttc aaatggtaga tgtgaaccat cgaacattac tgaagtatag
    10921 cctaattcga tacattccaa agcagcttcg taatcaccat ggtctaagtg gatagcaact
    10981 ggaacagtga tgttcattgc ttcaactaaa tcagtgatta aatctttaca gattttgtaa
    11041 ccacccatgt atttagctgc acccattgat gtttggataa gtactggtgc ttgttttgct
    11101 tcagccgctt caaggattgc ttgagtccat tccaagttgt ttgtgttgaa tccgccgaca
    11161 gcgtaaccgc ctttacgagc agcttttaaa aattcagctc ctgatactac tggcataata
    11221 aaattcctcc taagttttgt aaaactaaat ttgttaagac aattcttaac caacgaccct
    11281 attttaccaa acatttcggt agttttctac taaaatcaat gaaaatgaaa attttcaaga
    11341 agttttttgc aaaaaataaa atatccaata gcgctttaat cgaaaaaagt cttttgcttt
    11401 ctatcttttt tcctacaaat aataacaaat cagaaaaaga gaaaagtgac aagaaaattg
    11461 cttatcccct tctcttgcta cttttctctt tctatcaagt tcaaaaatta ttttgtttcg
    11521 tgatctgcta atgatgctcc gacaaatgct ttgattaatt tttgcgggcg atttggacga
    11581 gaaatcaatt ctgggtggaa ttggcatccc acaaagaatt ttttctctgg cagctctacg
    11641 atttctacta aacggttatc tggtgagaca cctgaaaaga ctagcccttg ttcttcaaac
    11701 aattgacgat atttgttatt gaactcatag cggtgacggt gacgttcttg cacaacatcg
    11761 gctccatcat atgcttcagc cgtcttcgtt ccttttttga ttttacatgg gtataatcca
    11821 aggcgtaaag ttccacccaa attctcgatg ttttcctgat cagccattaa atcaataata
    11881 ttgtttgctg tatctggatt cgtttcagct gatgacgcat cttctaatcc aacgacgtta
    11941 cgtgcaaatt cgacacaagc catctgcata ccaagacaaa tacctaaaaa cggaacgtca
    12001 ttttcgcgag cataacgaat cgcttcgatt ttcccttcta ctccgcgatc accaaatcca
    12061 ccaggaacca agattccatc tgcgtctttc aaacgttctt ctacattttc tctcgttaat
    12121 tcttgtgctt tgatccaatc gatctcgatg tctgaattat aggcgaaacc agaatgtttc
    12181 aatgcttcaa ctactgagat atatgcatct ggtaactcga catatttacc gacaagcgca
    12241 atccgtactt ttttcttcaa gttcaagaca cgttcttcta attctttcca ttctgtcatg
    12301 tctgcttctg gtgtatctag ttttaaatgg tcgcaaacaa tttgatccat tccttgtgct
    12361 tgtaaaacta atgggatgga gtaaagtgta tctacatctg gcgactcgat aactgcttct
    12421 ggttcgacat cacaaaattg tgccaatttg tttttcacgc tttgagaaac aggttgctct
    12481 gtacgtacca ccaaaatatt cggttggata cctaaaccac gtagctcttt tacactgtgc
    12541 tgcgttggtt tagttttcat ttctccagca gctttcaagt aaggaatcaa tgttgtatgg
    12601 atatacataa cattatctgt accgacatct gctttcattt gacgaagtgc ttcaaggaat
    12661 ggcaaagatt ctatatcccc tactgttccc cctacttctg taatgatcac atctgaatca
    12721 gtcattttag ctgctcgcat gattttttct ttgatctcat ttgtaatatg aggaataact
    12781 tgaacagttg ctcccaagta ttcgccctta cgttctttac gcaaaacttc agaatagatt
    12841 tttcctgtcg tcacgttgga gtatttgttc aaattgatgt caataaaacg ttcgtagtgg
    12901 cccagatcca aatctgtttc agcaccatca tctgttacga atacttctcc gtgctgatat
    12961 gggctcatcg tccccggatc cacgttgatg tatggatcaa atttttggat agttactttt
    13021 aagccgcggt ttttcaataa gcgtcctaaa gaagctgcaa caatcccttt tccgattgat
    13081 gaaacgacac cgccggtcac aaaaatatat tttgtcataa gttcaaaaac tcctttttaa
    13141 tttatgtgca ggaaaagtct cagacaataa acaaagctcc ctattcttca ataagaatag
    13201 ggagccgaaa ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa
    13261 gctgactaag ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt
    13321 tcgatatcct ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc
    13381 acataataag cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt
    13441 actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa
    13501 tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc
    13561 gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca
    13621 cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc
    13681 actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa
    13741 ggtaacgaat cacataagta aaaaataagg agatgcgaca aaa
//
LOCUS       DACEDO010000071        13404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEDO010000071 DACEDO010000000
VERSION     DACEDO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.071"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..422)
                     /locus_tag="IWO52_002269"
     CDS             complement(<1..422)
                     /locus_tag="IWO52_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0356517.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIA"
     gene            417..683
                     /locus_tag="IWO52_002270"
     CDS             417..683
                     /locus_tag="IWO52_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0356518.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(859..1428)
                     /locus_tag="IWO52_002271"
     CDS             complement(859..1428)
                     /locus_tag="IWO52_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0356519.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1502..1825)
                     /locus_tag="IWO52_002272"
     CDS             complement(1502..1825)
                     /locus_tag="IWO52_002272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356520.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(1979..2791)
                     /locus_tag="IWO52_002273"
     CDS             complement(1979..2791)
                     /locus_tag="IWO52_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356521.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3145..3993
                     /locus_tag="IWO52_002274"
     CDS             3145..3993
                     /locus_tag="IWO52_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0356522.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPRPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4138..5079
                     /locus_tag="IWO52_002275"
     CDS             4138..5079
                     /locus_tag="IWO52_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0356523.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLKKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFTHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5069..7240
                     /locus_tag="IWO52_002276"
                     /pseudo
     CDS             5069..7240
                     /locus_tag="IWO52_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7352..9523)
                     /gene="copB"
                     /locus_tag="IWO52_002277"
     CDS             complement(7352..9523)
                     /gene="copB"
                     /locus_tag="IWO52_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0356524.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9543..11729)
                     /locus_tag="IWO52_002278"
     CDS             complement(9543..11729)
                     /locus_tag="IWO52_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0356525.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11729..11938)
                     /gene="copZ"
                     /locus_tag="IWO52_002279"
     CDS             complement(11729..11938)
                     /gene="copZ"
                     /locus_tag="IWO52_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0356526.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11951..12391)
                     /locus_tag="IWO52_002280"
     CDS             complement(11951..12391)
                     /locus_tag="IWO52_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0356527.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12465..13004)
                     /locus_tag="IWO52_002281"
     CDS             complement(12465..13004)
                     /locus_tag="IWO52_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0356528.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3760 a         2823 c         2388 g         4433 t
ORIGIN      
        1 ttagcaatgt gccatccagc tacatgcaag tttgcgtttt cgatttctcc gaactgatca
       61 atgtgggcgt aatttgttcc atctgtcagc gtagaatttg cagcacctgc tcgagttgga
      121 tcaattactg gtttatcctc cgaagtagtt ggattatcat ccgtaaaacc atgagccaaa
      181 tcataggcta gtttttcttt acttacaccc atttcagaaa gataaccgta aggatctgta
      241 tgatcgcccc aaatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa
      301 ggagtgtcta atgttaatgg aataccatat ttcattgctg aatctcttgc caattcaacg
      361 tatactttat agttcttttc aaaagtttct ttatcatgtg tgtgttgtaa ctcgatttgc
      421 acaggacttt tggctcacga ggttgtccta ctctacatct tgctgataga tatgctgaaa
      481 ggaaaacacc aacattatgt tttgcttttt cagctagaac gagacaagca ggaatacctt
      541 tagaagatat caaagatttt ttaggtcata aagatgtttc cactacacaa gtctatgccc
      601 atatttctcc cgaagtgaaa aaaagatcaa tgaatcaact agaaaactat atagaagagc
      661 aaataaaaaa gcactcaaat tgagtgcttt tttaagctca ctatcactaa aactatcact
      721 aattttattt ataagcgaac atgtttctat cactgatttt ttaaatctag cgaaaaaccc
      781 ttatcttaaa ggcgttcgtt caattctttg gcaaggtctt caaagcctgg cttgcctaga
      841 agtgcgaaca tggttttctt atagctcatg tagaaaaatt ttcttgatga tgattttatc
      901 catcaattga gattgactgt tttgctctcc tatgatttca gctatgattg tccgattttg
      961 cggttttctt cttaactggt agttgatttt cccagtttcc atcaactcgc taagcatatc
     1021 cacgactccg ccaaatccgg tctttttcgt aataaaacct agtgctacca ttcgttcatc
     1081 tagatttttt gctgtaatcg tcacaccagt aatgagcgag tgctgtaatg tttgggccag
     1141 ctcttccatc ccttcagcat tggatacgac ggaagctgtt ttgttaatag gttcttcgtt
     1201 tatttctttt ggttctttcc tctccacttt cactgtttga cgaaggacat ctagttcttg
     1261 caagagcgtt tcgtattgtt cttgggtcag catcacgcca gctacttttt cacgattaaa
     1321 tatatatacg ccagttcccg cgtctctcgc ttgagcaaat acatccatcg gcgatcgttt
     1381 cacttctgtg atcgaagatg tgggaacttc taatttcttt aattccaatg gatttcctcc
     1441 tttactaatc gctttttatt atagcacaga ctgacttgtt ttgatctttt ttccaaaggt
     1501 tttatcggtt tttgcctaac cagtaattga tatcggtaag aagtctttcg ataccaaatg
     1561 aaggatggta cagatacgtt ttttgatctt cgttatggtc tagtggaaaa aaatgatcgg
     1621 tgatcactag atctgcatac cgaggcggct tatttcgagt acatacatgt atagagatcc
     1681 gttttcgatg tcttagcgtt ttgttcagta ccccagcaag tcttcttgct tcttggtgtt
     1741 catagcgaaa aaaaaagacg attttcactt caggagggtg gacatctaaa taccctcgtt
     1801 cctggagtgt tttcagtaaa agcatccccc actctactgc ggggtgtgca agtgcttgtt
     1861 ggatcgctag ccgaaattga tatagtttat ctttggaatt gggaaatttt tctaatgttt
     1921 cctgataata acgaacttct tctatcagcc ggtcatggat accaattacg taacgaagtc
     1981 attcttggta aagtactttt gtcagctttg atttgctttc gctctcaaat tcgctattca
     2041 gccaaaacag cgaatcaagc ttttcagtca caatcgtcaa tttccgatag tcttgtgtct
     2101 cttctatccg tttccaccgc ttttcagtaa tctggttaca gctcaagcgc aaaattgcta
     2161 gataattttt ttcttttatt ggcaaaatct gctcctctct aaatagcaat tcaggaaaca
     2221 attcaaagaa tagtgttcga ctgtctccta aaatctcgat ttctgccgga tcctttcttt
     2281 gcttcaataa ttggatgaac atctcagaaa aggactgctc tccatcatct gcaaaaaaat
     2341 gcggaaaatc tttcaaaaag caaaacagca gatatcttac ttttctttct gaaccaaaga
     2401 tctgtgcttt tttataagaa aaagaaaggt ggtactcgcc aaaccacttt ctcaatcgct
     2461 cttgcgtaga acgatcgtac ttctgctcgt aaggttcatt caatagcaga tttttcagta
     2521 aacaaaacgg gggtgattgg atcagaaaaa caaacaagag ataaggttcg ctatatcctt
     2581 gatcataaac taactgaacg gttttcttat gaagaatcag ctgaatggga cagtcgcttt
     2641 ccgccaaacg tttcagatgc cgatccactg ttttaacatc gattttcagt tcttcagcta
     2701 aatcagcttt tcgccagatc aaatctttca ttagattgat tagtgaaaat aaattctgtg
     2761 cattttttga taatagtttt tgccatctca tcgatcattt ctcacctgat ttctaaacat
     2821 attgtttgat caaaatgggt tcactcctaa aaagataaaa aactcccttt ctatttattt
     2881 aaatacgcaa atcttcctca ttatttttta ttttactcaa tttaatcgta ttctcatttc
     2941 aaaacgaatg tactttaaaa aaacataata agatcacttt gcttgtagga tgtattccct
     3001 tccattacaa tgaaataaat agtgatttta tgagaaggtg atgtgaaaat ggaagaaaaa
     3061 atagaagaat ccaaacaaaa aatcgatcag ttgatataag aattagaaaa agcggtcatc
     3121 ggcaaacgtt caactatcca attgatgatc gtggccctat tagcaggtgg tcatgtctta
     3181 ttcgaagata ttcccggtgt aggtaaaacg ttactggtca aagcactagc aaaagctgta
     3241 cagggaacct tcagcagagt ccaatgtact cctgatctgc tgcctagtga tattcttggt
     3301 ttttccgtat acaattcaca atcaaaagag tttgaatttc gtccgagacc gatctttacc
     3361 acgatcttgt tggcagatga gatcaatcgt acgactcctc gtactcagtc agcactttta
     3421 gaagcaatgg ccgagaatca tgcaacgatc gacaacaata cataccctct agacaatcat
     3481 ttttttgtct tagctacgca aaatccaatc gagtacgaag gaacatatcc tttgccggaa
     3541 gcacagctcg atcgtttttt attccgacta caaatcggtt atccttcttt tgatgacgaa
     3601 cttttgctac ttttggataa attcgaaaaa gtgttggata acttaaatga agtattgaac
     3661 agttatgaaa tagaagaaat aaaaacaaat gtggcgaatg tctttgtcag tcccgaagtg
     3721 gcatcttatg ccttgcagtt agtgtcagca acaagaagtc atgctgccat ccagcttggc
     3781 atcagtccac gaggaagcct ttcctttatt caggcagcaa aagcttatgc tttaatccat
     3841 gggagaaatt acgtcacgcc caaagatttg caggatttag ttccttacgt gttttcccat
     3901 cgcctgattt tttacgatcg ttcattgaag gaagacgaaa agaaacaaat cattcatact
     3961 cttgttgcac aaattcctat tcctgtgagg tagattgagg tagacaatgg aaaaaatcaa
     4021 agattatttc attaattttg tgttatttct ggtatatttc atcattgtgc tttttgctct
     4081 tgtattcaac aattctttag gctggttctt gttggccttt tttactcttt atgtatcatg
     4141 gagcttgatt tcattaagca gctttctaaa gaaactttcg gttacttgta cattgcagcc
     4201 actcgtttat cgcaaaaaaa gtatgcaggt tattttttcc atagcaaaaa agagaacttt
     4261 ttctttactt ctccctcgtt tacaaatcgg cttcccagca atctttgcag aaaaaaacaa
     4321 gagattttga ttcttacaaa taaaccgcaa gaagtccaat tgctttggca gccaaaagaa
     4381 agaggatgcc atgaaacatt aactgtcatt tttacagcct atgatagtct aggacttttc
     4441 agaaaaagtg caaaaagaga gatctctttt cctgtgactg ttctgcctgc tttttgtaag
     4501 attcaagcag aaaaattaca gagagttctg gaaaaaaaac agcagctgaa cgcgtaccaa
     4561 agaggtcttg attttcggga acatcgcgct tatcgtccag gtgattcttt caaccgaatc
     4621 gattggaaat tgtctgctca tactcaagag tggttatacc gtgaatacga ttatcaagaa
     4681 gaagaatgtt ctcctctttt ttgcttttgg ggttcgccca gtccaaaatt tgaacctctg
     4741 ctagacttgt atttttcttt atggcatttg aaaaaagaaa agcagccgga acttctgata
     4801 ttaggcaacc gcgcctttca tggcaaagac cccggtcata cctattttgc ttctttggaa
     4861 gctggcgatg agaaagcttt tttcacacac ttgaaaaaat atgcaaaaaa acaaatcgtc
     4921 ctctttgttc ctgagaattc tcctgaagtc tcagaagcta ttgaaacatt gtcaaaagat
     4981 cgaacggttt atcttgttta tttcgtagaa aaacaactca tggtccaaga aaaagacaag
     5041 atatgttcat tgccaggagg tgaatggatt gacgattaaa cgggaaaaat ggctgcgtgg
     5101 cgtttactcc tttgtgatac taagtgtagg tgctccgata tttgtagtag ctaatcaatt
     5161 gcaaaacagc taccttctta tcctaactat tggtgtccta tgccttattg tctgctgtat
     5221 tcctaaaaaa tgggtacgtt tgctgctttc tttgccagta atgataggtt gtttgttttt
     5281 atattttcct tcgaagacat tttccatatt atggatctta cagttttttt cgtcagttac
     5341 agaggaattc ttccatctat cccaaggtaa actttattat ttgccagaaa agacagcttt
     5401 ttttctcata atgttgctga tctatttatt tattacattg accgtggatt acgactattg
     5461 gtacgcaccc tttctttctt tgatggctta cttcatgatg cttaccgttt ttcggaaaaa
     5521 agatttactg tacgaaatga ttgcggtagt aactgttttg ttcgtgtatc tggcagcaag
     5581 gcaatttttc tccatccggt tgttgtcggg aaacactcga ttccagtcat tgactacagt
     5641 tctgcttatt tgtttcttaa cttttagcgc tatcttgcct ttatatcttc cgaaagtaca
     5701 ccaatctctc gaggaaacga gtgaaccgat ccgagaatat cttaatgatg aagggcttta
     5761 tgacacactc catgagtatc aattaaccgg aagtgcaaga acaggcttca gtgagaatga
     5821 tgaacagctt ggtggacctt tatatgataa tggcgaagtt gtttttacag cgaaccaaga
     5881 aggaaatggc tattggaaga tcgaaaataa aaatcattac accggttctg gatggataga
     5941 agatcaggac gcacccatcc aaccaatcca agaattccct gttgacatca cgagctacca
     6001 aaccgttata gaagacaaag aaaacattac attgacccta aaaaaacaag ttttcttacc
     6061 ttatccttat ggaaatacta ccttgactga tttttcatta gctagtggac aaacgcttta
     6121 ctatttgcct gaaagccagc gattcattcc tgtcccgaat gaatcgacac gttctgcaac
     6181 tttacaaaca gaaaggttag tttttacgag agaagaatta aaaatcagtc agttaaaagc
     6241 tgatgattta gaaaatcagc agacaaatct tcaattgcca aaaagtttgc ctaaacgtat
     6301 tgaggacttg gcggtcgaat tgaccaaaaa tgaagcaact ttaattcgta aagtcgaagc
     6361 tgtctaacag tatttaaaaa caagcggaaa tttccgctat tccaaaacgg ataccgggca
     6421 tactccaaaa gaacacgatt atgtggatta tttcctcttt gattcaaaag tcggctattg
     6481 tgacaacttt tcaacttcta tggtcgtcct tcttcgaaca cttggtatcc cagctcgctg
     6541 gacaaaaggt tttacaaatg ggctcagaac aggcacagat aatcaaggga atgaggaata
     6601 cagcattctc aatagtcatg ctcattcttg gccagaggta tactttcctg gatttggctg
     6661 gcttccattt gaaccaaccc cttcatttgc caatccaatg ggttcacaac cagttgctga
     6721 aacaaccgaa accgtccagc ctgctatcga acaaccaaat gaaagcgaaa atatggcaga
     6781 aactatttcc agtgagagat ctgaggtcat tccatcagaa gaaacaacac tcacaccagc
     6841 ttataaaatc gtcttattca gccttggagt gtgtttatcc gtcttgttgt tctttctatt
     6901 gttattcaga aaatctctgc gctggacaag ttatcttatc tatcttcatt tttttgcttc
     6961 tactaatacg attgcatatc aaaaacttct taaccctttg gaaacattgg taccgcgcaa
     7021 acccgcagaa tctttgcagg aatatagcct gcgaatagaa caagaattca agctagatct
     7081 gcgtttcatt cgattaacta ctcaatatga agcagaaata tatggacgac aagcagatcc
     7141 ggtaaaattc gataaaaaaa cctatttaga aataacgcat ataataagaa aaatggtata
     7201 caaaaaaatt ctgacaaaag ggagccgctt caaaaaataa gataaaattt ctggagaatc
     7261 tacttctgga atgccgagaa ccagctgatt gagtatcgat tactcgaaaa aaaggttgtg
     7321 acacgttttt tgtcacaacc ttttttcatt attatctgat tttcaatgtc aaagcattta
     7381 gagctacaac taccgtactt aacgacatca agacagcacc aactgctgga cttaatatga
     7441 taccaattgg cgcaagaatt ccggctgcta gcggaattgc aagaatattg taacctgccc
     7501 cccaccagag gttctggatc attttctttc gcgtttgctt ggctagatca agaaaatgca
     7561 aaatatcttt tggatcgctg tttgtcaata cgacatccgc agaatcgatt gcgatatctg
     7621 tccctgcacc gattgccatc ccgattgaag cacgggctaa acttggcgca tcattgatcc
     7681 catcacccac catgattact tttttccctt ggtcaaggta tttctggaca acagcttctt
     7741 tatcatctgg taataaaccg ccataatatt cttcgatccc taaataatct gctactgctt
     7801 gggcagcgtt tttattgtca ccagtaagca tgacaggagt gatttttctt tctttcaatg
     7861 ttcggataaa ttcttttgct tctgttttta ctacgtcacc taaagcaacg attgcagcta
     7921 gatgattttc taaaatcagg aagctgatgg tatttccttg ttcttggtaa ggtttcaata
     7981 atgcttgttc tacgtttaat ccgagacgct caacttcttt ttcattaact attttcactt
     8041 gttgtttttt gactgttgcc tctagcccga ctcctgaaag attttttagg ttttcagctt
     8101 gataaggctt gatttcatgt tctttcaaat aattcataat gccaacagcc aatggatgat
     8161 tggcattcgc ttccaaggct cctagatatt gaaggatttc atttttattg aacgcatcat
     8221 caagtacttc gacacccgtt acagtaaatt ttccttctgt tagtgttcct gttttgtcca
     8281 gcatgatcac atctagatca ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc
     8341 cgtttttagc tgctattgat gtggaacgag caacaactaa tgggatagct aaacctagag
     8401 catgaggaca ggcgatgata aagactgtta ccatacgttc taatgcatta ggaagatctg
     8461 tcaagaacaa ccaggcgatg aatgccagga tacccacaat taatgcgaca taaaacagcc
     8521 attttgccac tttgtcagat aatgactcta gcttggattt ttctccttgt gcttttcgca
     8581 ccatttccat aaccttcgct agataaccat tttcaccagt tccagttact tcgatttcga
     8641 tcgttccatc cccattgata gaaccgccaa tcactttgtc attggcttgt tttttgattc
     8701 ctcttgattc gccagtcaca gctgattcgt caacaattgt ttcacctttc aagattacac
     8761 catctgttgg cattttatca ccagaccgga cgatcaaacg atcaccttcg tttacttctt
     8821 tcaatgaaac tgtctcttct tttccatctt tcgtcaagcg tttcactgat tctggcagta
     8881 attctgctag tttttgtaaa gcattgctgg cattcgacac agcattcatt tcaatccagt
     8941 gacctagtag cataataacg atcaaagtag ccaattccca gaagaaatcc atgacgtgtg
     9001 tatgtggatt gagcagattt gcgataaacg aatagacact ataaatatat gcaactgtaa
     9061 tccccatcgc gatcagtgtc atcatagcag gacttttttg tttcaattcc atcttagctc
     9121 cacttaaaaa tggctgaccg ccatagataa atagtatagt tgctaagatc aatacgagcc
     9181 aatcagaacc aggaaacgtt acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa
     9241 gaatgattgg gatagctagg ataagcgata gccaaaattt ttgtttgaaa ttccccatgt
     9301 gcatggaatg atccatgccg ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt
     9361 tgctgtttgc tatatctcca tggttatgtc ccatgtgcat ggaatgatcc atctgactat
     9421 ggtcatgttt catctccatc tgtttttcat gagattctgt actttctttg tttactactt
     9481 cttttttatg ctcatgtttt ttctccatat cgttcccatt catttttact ccatcccttc
     9541 tcttactttt tcatgtgtcg atttaagctc agggagttca acaatacact gacggaacta
     9601 aacgccatcg ctcctcctgc aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata
     9661 ccaatcgtat tatagacaaa agcccagaaa agattttgtt tgatttttct caatgtcaac
     9721 ctagacaact cgattgtttg tgcaatggat gccaaactgc tattcatcag cgtcacatcg
     9781 gctgtttcca tcgcaatgtc cgttccactt cccattgcca taccgacatc agctaatgct
     9841 aaggccggtg catcattgat tccatcgcct gccataccga cttttttgcc atctttctga
     9901 agtttttcta cgtaggctgc tttatcctct ggaagtactt cagcaaagat atgctttgga
     9961 tcaataccaa cttgtttacc aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata
    10021 aaaacatcta atcctttatt ctggagctgc ttgattgctt gtttgacttc tagtttgatt
    10081 tgatcagcca cggcaatcaa cccgatcact tgcttttcat cagctaaaaa catgactgtt
    10141 tttccctgct gttctaattc taacgcgtgt tcttggtatt catcaaatga taggttcaat
    10201 tcaattaatc gtttacgtgt acccgcaaaa tagcgtactc cgttaatcgt accgctgatt
    10261 ccagcacctg ggtgggctgt aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt
    10321 ccgtattcca cgattgcttt acctagtgga tgttcagaag cttgttctaa agtataaaaa
    10381 atagatagga cttctttaga accgactaga tctgtaactt tgggctttcc ttctgtaatc
    10441 gttccagttt tatccaaaac aatgctgtct aagtgagcag ctgcttctaa tgcttctccg
    10501 cctttaatta aaattccatt tcgtgcacca acacccgttc ctaccataat agctgttgga
    10561 gtcgctaaac ctaatgcaca tggacacgca atgactaata cagagacact atggatcaat
    10621 gcgagttgcc aatcaccagt gatcaaacct gtagctatga gcgtgataaa cgcgatgact
    10681 aatacgatcg gaacaaaaat ccctgagatc cgatctgcga ttttttggat tggggctttg
    10741 ctcccttgag catcttccac catttgaatg atttgcgcta atactgtctg cttgccgact
    10801 tgtgtaactt ccatgtgaag tagaccattc gtattgattg tcccgccaaa gagcgtgtca
    10861 tccggattct tttctacagg aagattttca ccagtaagca tactttcatc tatcgcactg
    10921 cttcctgaaa taattcgacc atctgctgga atctgctcac caggacggat gactagttga
    10981 tcaccaacga ctacttcttc aatcgctaac gtttcttctt tcccgtttct tatcacttgg
    11041 gcggtttttg tttgaagaga catcaattgt ttgattgcat tacctgtttt cgtttttgct
    11101 gtatgttcca aatatttgcc gagcaaaatc agcgtgatga tcatactgct gctttcaaaa
    11161 taaagatctt gggggtgtcc acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc
    11221 gatgttccaa ttgctaccaa tacatccata tttggtgctt tcgtcttgat tgcatggtac
    11281 gcacctttat aaaaacgagc accgacataa aattggacag gagcagacag cactaactgg
    11341 acgattggaa aatgaaaaaa gtgaacaatc gccgcatggc ttcctaacat catagcaatc
    11401 attgacaaca tcaacggcaa agtcaaaacc gtactcaata taagatcgaa tagcattttt
    11461 ttcaaatacg cttgcttttc ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt
    11521 gcaccatagc cgatgttttc tacgctttgg attaattttt cagtggtcgt cccttcatat
    11581 ttgacacttg ctttttcagt ggccaagttg actgtggcat tgaccacacc aggttgtccg
    11641 ttcagctctt tttcgatcct agcagaacaa tttgcacaag tcatacctgt aatgacgaac
    11701 gtatccattt tggtttctgc tgttgccatc aaatcacctc tgctttataa cccaatccat
    11761 tgattgcttg acaaatcttt tcggattgaa cttttgtttc atcaaacttg accgtaccgt
    11821 ttgctttttt caaatttact ttcacttttt gcacgccgtc aagtgcgctt actgtttctt
    11881 ctactcttgc tgcacaatga ttgcagctca taccttcgat tgaaaattgt tgtttcattt
    11941 tttcttcctc ctattgatgt tgtttacatt cacattgacc tgggatacag ttacattcga
    12001 tcgtatctac tggttgcttt tgttccagtt gtttcaaaat ctcattgata tcttcttgag
    12061 tcaaagtggc ttcttctact agatccgcaa tcgtctctcc aatccttttt gcacagatat
    12121 gggaaaaaag attttccgtc gcactgcgaa ctgtttccat ctccgagaca gctggatgat
    12181 aaatgaattt ttttccttct tgttctgtcc agatcacttc tttttttacc aaacgaccca
    12241 gcaaagtttt gatcgttgct ggtttccaat ccatcgaatc agctaagatc tgcgtgattt
    12301 cttgggctgt cgtctgtcca agtgtccaaa taacacgcat gatctcccac tctgaatcac
    12361 tgatctttac tggcgttttg actttttcca tttatttcac acctttcgtt tacaaatgta
    12421 atcattatct tatgaataca gattacactt gtaaacaaaa actgtcaatt atttttgctc
    12481 tctattttca atttagttaa aaattcttgt ttctctcgtc ttgttttaag gatgatcagt
    12541 ttatttcttg cattagctac ttggatcagc tgctgtattt tcggttcatt gttttcttca
    12601 aaatgccaga taaaagataa aaattctaag tattccctgt ttagtttctc cgaaaatgat
    12661 tcaggcatat ctggccgaga ttttttgttt atctttcttt ttagagaacg ccaaactact
    12721 cggtagatgg cgagataacg cggcacttta agccaaatga tcttatctgc ttctttaatc
    12781 ctttcctcta atgtagaagt ataatttcct tcaacgatcc atgacggatt agataccata
    12841 aacaattgct gttcttgcaa aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc
    12901 cataaaaggt ctagcggcag tagcggaaag tcggtgattt ctttgatctt ccttgcgagg
    12961 gtcgattttc ccgacccact ttgtccaatc acgatgatct tcattcttct ccctcacttc
    13021 tctttggaat ttggttcgat cataccactt ataacgccgc aaaaaaacaa gggactgtga
    13081 caaaagtcgt gtcaaagtcc cttgtttcga ataaatggtg ttcaatcagc tgaccttcag
    13141 cattagctca aaaagcggca aaatatgagg agctattttt ccgtttgttt ctctaatact
    13201 caggtcaaat cgctgatttt acaacctctt ccttaaacgg tgttctcctg ccaacacctc
    13261 ggtcacaagt cacaatagtc aacaaacatg agaagttgtt ttttgaaatt gctccttgtg
    13321 actcggagtt aaacggcagg ctcactacct ctttcttaaa cggtgttcca aagcttgcat
    13381 ctgcggtaat aagcccagcc aaac
//
LOCUS       DACEDO010000072        13345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEDO010000072 DACEDO010000000
VERSION     DACEDO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.072"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(91..534)
                     /locus_tag="IWO52_002282"
     CDS             complement(91..534)
                     /locus_tag="IWO52_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0356529.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(729..2069)
                     /locus_tag="IWO52_002283"
     CDS             complement(729..2069)
                     /locus_tag="IWO52_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0356530.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2351..3247
                     /locus_tag="IWO52_002284"
     CDS             2351..3247
                     /locus_tag="IWO52_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0356531.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3390..4331)
                     /locus_tag="IWO52_002285"
     CDS             complement(3390..4331)
                     /locus_tag="IWO52_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0356532.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4457..5578
                     /locus_tag="IWO52_002286"
     CDS             4457..5578
                     /locus_tag="IWO52_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0356533.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5836..7338
                     /gene="lsa"
                     /locus_tag="IWO52_002287"
     CDS             5836..7338
                     /gene="lsa"
                     /locus_tag="IWO52_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0356534.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7405..7932
                     /locus_tag="IWO52_002288"
     CDS             7405..7932
                     /locus_tag="IWO52_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0356535.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            8144..9205
                     /locus_tag="IWO52_002289"
     CDS             8144..9205
                     /locus_tag="IWO52_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0356536.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9208..10104
                     /gene="murQ"
                     /locus_tag="IWO52_002290"
     CDS             9208..10104
                     /gene="murQ"
                     /locus_tag="IWO52_002290"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0356537.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            10118..12100
                     /locus_tag="IWO52_002291"
     CDS             10118..12100
                     /locus_tag="IWO52_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0356538.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12170..12616)
                     /gene="nrdI"
                     /locus_tag="IWO52_002292"
     CDS             complement(12170..12616)
                     /gene="nrdI"
                     /locus_tag="IWO52_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0356539.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            12827..>13345
                     /locus_tag="IWO52_002293"
     CDS             12827..>13345
                     /locus_tag="IWO52_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0356540.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVA"
BASE COUNT         4354 a         2418 c         2805 g         3768 t
ORIGIN      
        1 gattcacggt gttccagaag tagcttcttc ttatttcttg aagttgatca cttctggaac
       61 atcctctttt tttatttgga tggataatgg tcattccatt tctttgatca tttttttcat
      121 tttgtcgatt tcttcttctt gtgcctgaat gatttcttcc gctaattttt ttacttcagg
      181 atcttttaaa tcagctcgtt tacttgtcaa gatagcaata gagtgatgag ggatcatcgc
      241 cttcatccac gccgtatcgt tgacagttgt ttggcttcgt accataaata aggaaccggc
      301 aaaaacaaga agactagcgg ctaaaatcat agcatttatc tttttgtttt catacatttt
      361 ccacatgaac aacaacatga tgactgccat gactgcaccc atcatcaacg ccatgaatac
      421 acgtgtgcga ctgaagaaga tatgatcaag cgcataaaca ttcaaataca tcataccaaa
      481 catgactacc gtcgaaacac caatcatcaa caaaaacttc caatactttt tcatttaaaa
      541 caaactcctt tttcttcatt cctttcttct atcatgtcat gagcgtggag aacaatcaat
      601 taatctgcac agatggtttt tgaacaccgt ttattcggaa aaaagaactg caacaatttt
      661 tgtcgcagct cctttttttc aatagctcca gatattcaat ataaatagaa taaaattctc
      721 tattggtttc attcattcgt caagacgaaa gcatcattct ttttttgata actgaaataa
      781 atgaacaggt aaagcaaacc ggtcaataca gttaccccaa tggagttcgt gaataggata
      841 ccaaggaaaa tcaaccataa gactgctaat gcaaaaaagc tgattccttt gtcccataac
      901 gttcctttcc ttagtgtcag tacaaaggac gcagcactga taatgatcca gatcaataat
      961 actgtatagg aaagagaacc tgccagatag ccaaataatt tgtcaccgac aaaataagaa
     1021 agagccacac ctagataaag aacactggta caaaacagca cagcacgttg cggtacttga
     1081 tgcttgttta aaacagctag ttttctagag gaacctttcc gatctttcaa gcggaagaac
     1141 aaaattctcg aagaagcata tacacctgaa ttgatagacg agaacagagc aagaacaatc
     1201 acgaaattga cgatatctgc cgcaaatgga atattcattt tttcaaatac catcacaaaa
     1261 ggactaacgt tcgtgcctgc taaagtattc caaggaaaaa taatcaaaag caaaaataaa
     1321 ggaataatgt aaaaggaaat gattcttccc atgacgcctt tgattgcttt aggaatcgca
     1381 cgtttaggat catctgcttc gcttactgtg atagcaatca actcagaacc gccgtaagaa
     1441 tagataacta caagcaaaga actgatgatt cccttcatac cgtggggagt aaaaccacca
     1501 tgatcagtta attgttgaag ggtagggaaa acaccagtac ccaacatttg ctgaccgaca
     1561 agataagcag caaaaatgat caataggata atgacactga ttttagcaaa ggccagccaa
     1621 tattctgtct ctgcgaataa tcgtaccgaa taaagattga tgatagtagt taaaacagcc
     1681 aacataaaaa caaataccca agctgggatg tgggggaacc acaactgtaa aaaggaagca
     1741 gctgcaacag cttcagcaat gatattgata atccacatgg accagtagac ccaatcggta
     1801 aaatcggcag catgagcacc tagatatgga cgaatcaatc cagataatcc gtggtgcgta
     1861 tcaccaccac ttaaaacaat ttttcccaga cctttcatca cgatatataa aattatgccg
     1921 ccaatcaaaa aagcgatcaa tacagaaggg ccggcgattc caatcgcttc gccacttcct
     1981 ttaaatagac ctgcaccaat cgctccacct aaagctagca tggtgatatg tcgggagcta
     2041 agttgttttt tcaatttttt atcttccata aataacctct ttcttttaaa ttcacaatca
     2101 aaatgcatag taagaataaa tagtaagaat aaatagtaat aataataatg actaatgata
     2161 acaaattttc agataattac aactattcag aaaacttttg ggcatatttt aatggaagaa
     2221 tgatttattt catcgtattt tcatagaaat gttaaatttt tataaaaata ataggaagcg
     2281 ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca gagaaagtga
     2341 ggcggaacag atgaacaaaa acgacgtatt gctaattgcg aaaaataaat cactttattt
     2401 ttttccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac agtcggaatt
     2461 cattaaatca gctcgacaac agttggcaaa ttatcagctg atcgtttttt tagattatgg
     2521 atttgatatg gagatggctc gacgcgtgcg tccatttacc aatgcaaaaa tcgtgttatt
     2581 tttttggaac cactttaaag cagaacacga ggcgatgctg aagcaggcac aagatgaacc
     2641 ggcaatcgat gagatttatc attttgattt tttagaagcg aaagaacttg gactaaaaca
     2701 taatagttcc ttttattcga aacatatgga tttttcttct agcgaaccaa agacggatct
     2761 attttttggt gcaacaaata acggcagaaa agaacgggca gaaaaatata agcaggaatt
     2821 tacaaaaaga ggtgttacga ccaattactt tattcttccg cttcgaggaa atgaacaacc
     2881 tggatacctt acttatgaag aatatctgaa aaaaaccggg gaatcgaggg ggattctaga
     2941 actgttgcgg gaagggcagc aaggagtgac tttgcgaacg tttgaatcta tgtattttca
     3001 aaagaaacta gtgacagata atcaagccat agctcattat cagttttatc attctgaaaa
     3061 tatttttttg cttcaagaac gtgatttaga tgaattaccg gaatttttgc atacgccatt
     3121 tcaaccaatc ccatcagaac tgttggaatt ttttgatgca gaaaattggg caaaacgttt
     3181 tttcactact gactccgcta tttatgaaca atatgaatac gatctttcct ctgttaaaaa
     3241 taaataaaag tgccaaccaa tcatagatga aaataaacaa gcgcaattct tatggcaaca
     3301 tgagaattgt gtttttcttt ttgaactttc atgggggtaa aaaaatgaga accagctgct
     3361 gatccagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3421 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3481 cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3541 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3601 tggtacactt cttcttgatc taatgtgatt ccttcttttg ctgcgataga agcaaactca
     3661 gatacaatcg ttcgaaccat gccctctgcc gctttttgcg cacctaattc agcaatatta
     3721 caatccaata tcgtgcataa tccatttaat gttccgttta cacaagcttt tcgccaaatg
     3781 gaataacgaa cattatgact atagattgga ttcaattttg cttcttgaaa gacttctaca
     3841 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3901 cctaataatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     3961 aagatatttt tctttggtac atatttttcc agtacatctt catgacctaa tccattcagt
     4021 aaacacaaaa cataagtatt ctcagtaata atggattgga tcgaacgaaa catatcatct
     4081 aattgattcg cttttgtcaa ggctacgatc aaatccacgt gtttgttgct ttcgatgatt
     4141 tcttctggtg aatagattgg cagttttgca actacttctt tgccattaaa gtcagcaatc
     4201 aatccatttg tacgaatggc ttcaatgtga gcaggccagc gatcgatcaa gacgacctca
     4261 tttccgcttt gatgcaacat taatccgatt cggctgccca ttgctccggc tccagctatt
     4321 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4381 cagaagaaaa tagcattgtt ttgtttgaaa aaagtgaaaa aaagtgtctt acttaagaag
     4441 aaagtgaggg atatagatgg atattttggc ggcaatcgat tcatttaaag gaagtgcttc
     4501 ttcatttgaa ttaaatcagg cagttctaaa tggttgggaa gaccgtaaag gaagaaaaat
     4561 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcgg
     4621 gggaacctgg cgtacgattg atgggatgga tctcttattt cgtcagcgaa aaatcagtta
     4681 tttattgacg aactggcaag gaaaaaatac cgcagtcatt gaatctgcac aagtattagg
     4741 attagattta cttgataaac caacggatca aaccatccga cttgcttcta gttatgcctt
     4801 gggagagctt atcaaagata cactgcttca agaagtggaa caaatcattg tcactttagg
     4861 aggaagcgga tgtagcgatg gtggtctagg attactccaa tctttgggtg cttctttaga
     4921 agggtacaca gaaggaaatc ctttgttatc tactagaaaa cttcatttgg atcgaaggaa
     4981 cgttcttgtt tctaaagtaa aagtgatcat cgctgcagac gtgaccaacc catatacagg
     5041 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaacggata cgacgttagc
     5101 tttcttggat aaacaagctg aaaagattgc tcagcagata aaagatgttt gggaggtgga
     5161 tttagatgac atcccaggaa gcggagcagc aggcggtatt ggcggagccc tttttttgtt
     5221 aggagcgaag atggtctctg gttttgagtt ggtcagccaa atcactggat tagaaaaaca
     5281 gatcgaacaa gcggatgtca tcattacagg agaagggcgg attgatcagc aaacaatcca
     5341 cggaaaagtt ccatatggcg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5401 tatttgcgga agtagagacc gagagctgga aggactcttt gattttctcc ccattgtctt
     5461 aagcattcaa ttaggaccaa tcgatttaga ggaagcaatg gaacacaggc aaacattaga
     5521 aaaaacagaa atcttaggac agacactcag cagatttttc aacattctcc cgaagtagtt
     5581 gacaaaaaca atttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5641 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5701 agggagcagt gtgcagattt tcgggattgg cttggttctt tcgatgtatg ggtctttttg
     5761 tgtgccccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
     5821 cgaggtgatg gaataatgtc taaaatcgaa ataaaaaatc tgacattcgg ctatgacagc
     5881 caaggcacat tattatttga acaagccaat ctaaactttg acacacaatg gaaactagga
     5941 cttatcggac gaaacggtcg aggaaagaca actttactga atatcctgca aaacaaactc
     6001 ccttatcaag ggcaagtgat ccatcagcaa gcgtttgtct atttcccgca gcaaacaaaa
     6061 gataaagagc gtttgaccta ttatgtgtta aatgagatta tggattttga gatatgggaa
     6121 attgaaagag agctccaact gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6181 acactatcag gaggagagaa gacaaaagtc ctactggcac tcttatttgt ggatgacact
     6241 catttcccgt taatcgatga accaacgaat catttggata tctctggcag aaaacaagta
     6301 gcggcttact tgaaaaagaa aaaacaagga ttcatcgtgg tgagtcatga tcggggattt
     6361 atcgatgaag tagtggacca tgttttagca atcgaaaaga gccaactgga actttatcaa
     6421 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatagctaaa
     6481 aatgaaaaat tgaaaaaaga agtcagcagg ctaaagaaaa cagcagctga aaaagcagaa
     6541 tggtctcgtt cccgagaagg ggacaaaaca aagaaaaaag tcggattcat cgatacggaa
     6601 tctaggcgag tgaataaagg agcagtaggc gctgatgctg cacggacgat gaaacgatct
     6661 aaagcaatcg tgaatcggat ggaaacccag atcagtgaga aagaaaaatt attaaaagat
     6721 atcgagtata tcgatgcttt gacgatgaat agccaagtgt ctcatcataa acggctgtta
     6781 agcgtagaaa atcttcaatt agggtatgaa aatctgttat tcgagccagt tcattttacg
     6841 atcgaacctc atcagcggat ggcaatttca ggtcctaacg gcgcgggaaa gtcatccatt
     6901 atccattatc ttctggggac attcaacggc aaggttgtag gagaaaaatc ccagcccaaa
     6961 catttgacca ttagttacgt acgccaaaat tatgaagaca atcgagggac attggcggaa
     7021 ttcgcgaaga aaaaccgagt agactatcaa gcatttttga ataatctccg aaagcttggg
     7081 atggaaagag atgtttttca taataaaatc gaacagatga gtatgggaca acggaaaaaa
     7141 gtggaattgg ctaaatcttt atcccaacca gctgagctct atatatggga tgaaccattg
     7201 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgattttgaa cgtgaaacct
     7261 gctatgttac tagtggaaca tgatcaaacc tttctagata aagtatctac tgagattatt
     7321 tctcttgaga aaatctaaac cttgtaatct caaagacaac aaggtatggt taaggagaac
     7381 tttgtcaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7441 aacgcacaag gattagtttg cgggcgagca gatgtgccgc tgactgaaaa aggacagatg
     7501 caggcacagg cactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7561 cattcccctc ttcaacgagc aagagataca gcacaagcag tagttgatcg tctcaatttg
     7621 ccgatgaccg ctgatgagcg attggtagaa atggattttg gggattatga cggactgcca
     7681 agcaaagatg aaaactttca aaaagcgcgt ttagcgtttg ctgtacgttt cccaaatgga
     7741 gagtcggtct tagatgtgta cgcacggatc gttcctttat tgaaagaatg catggaagac
     7801 gaagaaaatg tctacttgtt agtctgtcat aatgccttga taagagtgat caatgcttat
     7861 tttcatccaa tgcctaatga agggttcttt acattcatgg tagacaatac agaattaatt
     7921 tcttatgagt aattagactt ttgtgttatt taaataaaat gtttcaaggc actgcttttg
     7981 cttagcagtg ccttttttgt ggctgtgtca attcttttgg cattataaat ccaattttta
     8041 gaaataaaat ttaattattt taacaaaagt attgaaattt tgtttcttta gatatatact
     8101 ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg gcttttcaat
     8161 atttatgaat gattctttgt cagaagagaa gaaacagtat atccgccaaa tggcatccaa
     8221 tggctttgtc ggtattttca cttccatgca tataccggaa gatgatgttt cggcttatca
     8281 aaatcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagagt tgatggtcga
     8341 tatttcagga gaggcattga aacgagctgg cttttcatta gaagatctaa cacccttgaa
     8401 agcaattggt gtaacagggt taagaatgga ttatcatatt tctaatcagc agatcgccga
     8461 ctggtcacat caattgaaaa tcagtttgaa tgccagcacg atcacgccta atgatatcga
     8521 tgagttgaaa gaagcagcgg ctgatttctc acagattgaa gcttggcata attattatcc
     8581 acgtcttgaa acagggttgg acaaagaatg gtatcaggaa aaaaatcaat ggctcaaaac
     8641 tcaaggattc cttgtacaag gctttgtacc aggagataca gagttgagag gtccactgta
     8701 tcaaggatta ccaactttgg aagaacatcg agggatccat cctctggcag gtgctcttga
     8761 tctattagca agtaacacgg atattgttta tatcggagat gcaggtctgt cagaacacgt
     8821 tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata cggaacctgt
     8881 agatgaagtt ttttacgaat atatattagg aaaacatacg aatcggcaag atgatgcaag
     8941 agatgtcata cgaagcgcag atgcgcgttt tcgagagatt ccgccaatcc cagtaagaaa
     9001 tacagctaag cgtatgaaag gatctattac tttagataac gaaaagtatt tgcgctatat
     9061 gggtgagatc caattaacta aatatgattt gccagcggat gaaaaagtga atgtagtagc
     9121 caaagtcatc aaagaagaac taccgctgat caaccaaatc aaagctggta tgaactatca
     9181 atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa cctaacaacg
     9241 gaacgcagaa accaagaaac ttttggatta gatgaaatga gtatttctca agcagtccaa
     9301 ctcatgaaca aagaagatca gaaagtggcc gctgctgttg aaaaagaact gccaagtatc
     9361 gagccagtga tcgccaacac gattgaatcc tttaaaaaag gtggtcgctt gatttatatg
     9421 ggagctggaa cgagtggtag acttggtgta ttagatgctg ctgaatgtgt accgacattc
     9481 ggtgtagaac cggaaatggt cataggccta attgctggtg gagaagaagc aatgactgta
     9541 gccgtcgaag gagccgaaga ttcttatgaa ttagggcaag aagatctaat caagctgaat
     9601 ttaacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc atatgtgatt
     9661 ggtggattag attacgcaaa atcgattggt gctgttacag gaacgatttc ttgcaataaa
     9721 caagcagaaa tcagcgagca tgcggatttc ccaatcgaag tcgatgcagg accagaattt
     9781 ttgacaggct ctactcgatt gaagtcagga acagcacaaa aactgatttt gaatatgatt
     9841 tctactatca gtatgatcgg tatcggtaaa gtattcaata acttgatggt cgacgtcaaa
     9901 ccaacaaatg aaaaactaat tgaacgctca aaacgaatca tcatgcaagc gacagaagct
     9961 gactacgaaa cagctgaaaa gtactttgag gaagcagatc aaaatgtcaa actggctatc
    10021 gtcatgattt tgacaaatag tgataaagag gcagctcaag caaaattagc tgaagcaaac
    10081 ggttttgtaa aaggaacctt atgaggggga aaagaccatg gcaaaaaacg aattgacttt
    10141 ggaagaactg gcaaaacaaa tttatcaagg agcaggcggt atgggcaatg ttgaaagcat
    10201 tgtccactgc atgactcgtg tacgtatgaa ggtcatcaat gatgatcttg taaatgtctc
    10261 ggaattaaaa gcgattccag gagtacttgg cgtagtagag gatgaacagc tccaagtcat
    10321 catcggacct gggaaagtga acaaagtagc gcaggaaatg gttgacttag caggcgtgaa
    10381 attaggagaa aaacttcctt ctgctagtgc aactacagca agttcttctg catctggaaa
    10441 agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga aacaaaaacc
    10501 ctctaaaatc aaatcggtat taaaagacat ctctaatatc tttgtaccaa tgatccctgc
    10561 atttgtcggc actgggatcg tggcagggat tgccgcagtt ttagcaaact tagtcacagc
    10621 tggaacacta gacgcaggaa catggcaaca atatatcgat gtgatgaaca tcttgaaaaa
    10681 cgggatgttt agctatctag ttatctatac aggtatcaat gctgcacaag tttttggtgc
    10741 aacgccaacg cttggtggag ttatcggggc tgttgtgatg ctgacaggga tgaatccaga
    10801 agcaccactg aaaaacttgt ttaccggtga agctcttgca actggacaag gcgggatctt
    10861 aggtgttatc ttcgcagttt ggttattgtc aatcgttgag aaaaaactgc ataaagtagt
    10921 acctgatgcg gtagatatca tcgttacgcc aactctttcc ctgattgcca tcggtctgat
    10981 cgaaattttc ttcatcatgc ctttagcagg gttcatttct gacggcatgg tcggtgggat
    11041 caactgggta cttggtgttg gaggagcatt tgccggcttc gttctaggta cattattctt
    11101 gccaatggtc atgttcggac tacatcagat cttgacgcca atccatgtac aaatgattga
    11161 tgaaactggt cgtaccttat tgcttccaat ccttgcgatg gccggagcag gacaagtggg
    11221 tgcagcattg gcattatgga tccgctgcaa aaaagacaaa gaattaacag aaatgatcaa
    11281 aggggcttta ccagtcggaa tcttaggaat cggcgaacca ttgatttacg gagtaacatt
    11341 acctctagga cgtccattta ttactgcttg tatcggtgga ggtatcggtg gggcagtgat
    11401 cggaatgatc ggaaatgtcg gagcaatcgc tattggacct tcaggagccg ctttgattcc
    11461 attgatctct gatggtaaat ggttaggcta tatccttggt ttattggctg cttacgctgg
    11521 tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa aagaagaact
    11581 agcgaccgaa acagccacag taaatgaagt acctgaaaaa gcagtacctc aagcttcagc
    11641 tgaaagtgtg actatcttat catcagttgc tgacggaaca gttgtttctt tagataaagc
    11701 aagtgaccca gtttttgcac aaaaaatgat gggtgaaggt tactttgtag aaccagaaaa
    11761 cggacatatc tattccccgg ttactggagt aataagttct gttttcccaa caaaacatgc
    11821 tatcggcatt acgacaccaa atggcttaga aatcttgctt cacatgggca tcaacacagt
    11881 tgacttaggc ggtacgccat tcgatgtgaa agtaacagaa ggacagcaag tgacacctga
    11941 tacgcttgta gcagaagctg acttagaagc aatcaaagca gcagacaaag aaacctccat
    12001 gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa aaacaggtaa
    12061 agcgaaagca aaagcacctg tgatggatgt caaagcttaa taagaataga aacaatagag
    12121 aacataaaaa gaagaagccg aaatttattt ttcagcttct tcttttgtgt tatttatttt
    12181 tttctgccaa aatttgataa acgcgtgtca catcagatgg tgttccccgt aattcataat
    12241 ctgccaagaa aggaaagccg aatctttgtg cgtattgttt ggctgtcaga caatattgat
    12301 tgttgaagtt tttattaccg ctaccgacta caccaaggca aagtttatga ttgtcttcgt
    12361 attccagata ttcgcgcatc gtttcggtca agatttctgt gtcaccgttg tctaacccat
    12421 tcccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat
    12481 cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca
    12541 ttttttcagc atattctgct agatgtttgg caaaagcacg cgtatttcct gaaatcgata
    12601 tatataaaat gttcatggat catcccccaa ttctttccct ttagtatagc atctttttaa
    12661 gtttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata atgagaaaaa
    12721 agtcggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat aaaaaaggtt
    12781 tgttatactt tttgataaaa gcggttttac tttggaggat gcttacatgg atacagagca
    12841 acagaaaaaa ggaattggtt tgattccact tgctgcctta gtgattggtt cagcgattgg
    12901 aggtggcgta tttggtatta tgacggacat ctccggatcg gctggtggcc cagctatctt
    12961 cagctgggta ctcgtaggaa cagcaatgtt gatgctctcc ttatcgatca ataatatcaa
    13021 taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg
    13081 tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag gaaacgtggc
    13141 gttcgcaaca ttgttgatga gtgcattagg ttatttcttc cccgtattcg aaggtggtca
    13201 aaatgtggca tcgatcgtga tgagtacgat cttcttatgg ggatatgcat ggctagtgaa
    13261 tcgtggagtt gaaaatgcta gctttgtcaa tgctatcgtc acattttgta aattagtgcc
    13321 attattttta tttattgtag tagct
//
LOCUS       DACEDO010000073        13250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEDO010000073 DACEDO010000000
VERSION     DACEDO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.073"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            186..1055
                     /locus_tag="IWO52_002294"
     CDS             186..1055
                     /locus_tag="IWO52_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0356541.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1264..2289
                     /locus_tag="IWO52_002295"
     CDS             1264..2289
                     /locus_tag="IWO52_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0356542.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2328..3278
                     /gene="manA"
                     /locus_tag="IWO52_002296"
     CDS             2328..3278
                     /gene="manA"
                     /locus_tag="IWO52_002296"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0356543.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3372..5075
                     /locus_tag="IWO52_002297"
     CDS             3372..5075
                     /locus_tag="IWO52_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0356544.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5077..7110
                     /locus_tag="IWO52_002298"
     CDS             5077..7110
                     /locus_tag="IWO52_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0356545.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7121..7561
                     /locus_tag="IWO52_002299"
     CDS             7121..7561
                     /locus_tag="IWO52_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0356546.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7761..8888)
                     /locus_tag="IWO52_002300"
     CDS             complement(7761..8888)
                     /locus_tag="IWO52_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0356547.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8891..9568)
                     /locus_tag="IWO52_002301"
     CDS             complement(8891..9568)
                     /locus_tag="IWO52_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356548.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9832..11598
                     /locus_tag="IWO52_002302"
     CDS             9832..11598
                     /locus_tag="IWO52_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0356549.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11613..12050
                     /locus_tag="IWO52_002303"
     CDS             11613..12050
                     /locus_tag="IWO52_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0356550.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12063..13214
                     /locus_tag="IWO52_002304"
     CDS             12063..13214
                     /locus_tag="IWO52_002304"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0356551.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4498 a         2303 c         2735 g         3714 t
ORIGIN      
        1 cttattactt gatgcagaac agctttttca caacctcttt ttataagagt ttttactcat
       61 taatttacaa aaaacaagca aaatgcgaat tttctcacat tttttttaac aaaatcaaag
      121 ttattgcttg aaagaagaag aatccatcgt taaaataaag cttgacgtag aggggagcaa
      181 gaaatatgaa agattttcaa ttgggggctg ccacctattt aaaaagtgta gccattcggg
      241 tgaaagaccg agataaaatg attgattttt acaagaacgt tgttggcttt gatttgaaaa
      301 gagaagaaaa cgaattagcc attttaggaa cagaagaagc tggaagtgaa atgttgctgc
      361 tggaagaaag tcctagagcg aatgatcaca tgggggtgat caaaaagctg aaccgttttt
      421 cattgattat ccctagcaca gaagaaatgg cagatatcct ttgtcgtatc cgtaaaatga
      481 attatccaat caaagaagct ttagaagatc atggacgtat ggggattttg ctcgtagacc
      541 cagaagagaa tgaattggaa atttaccatg aagagaataa gaacgaccaa agagaaattc
      601 cagaaacaat ggatcaagaa gcattattaa gaaaatctga agaaaaattc agtaaactat
      661 caacaggttc ttattttgaa aaaatccatt taaatgttac taacttaaca aaagaacgcc
      721 aatttcttaa tcatgtactt ggtttgaagg tacataatga agaagagggc atccatgtat
      781 tgaatgaagg agatttccat gttggtttga cagaagctag cggaggcgca attgatttgc
      841 ctacagatga agtgattgga cttgattttc tgaaattcat gatcgatact gaaagcatgg
      901 aaggactgat tgctcacttg cagcaactat cagttgattt cttcttagac aaaaagcaca
      961 cagtactcac aatctatgat gctataggaa tcgaatggtg gttcgtcagt gagccaaaag
     1021 aaaagaataa aactgacatt cgatgcgacg cttaactttt tctttcttaa cgattgtcgc
     1081 atgtctcgtc attttatgaa cggtcatact atcgcttatt ctagagatag aaagagaaaa
     1141 tagtataccg ggggaaactg cgcggttttt cgcgcagttt ttttgtgtaa aaaaggttga
     1201 ttcctcatag attaagcttt aagcttggac tgtaagaatc tgatagaaaa gcaggagaaa
     1261 aagatgtatc cacacacgaa acagcttatt ttgaaaaaga tggaagaagg attttatcca
     1321 ggtgtagcag tcgccttcat aaaagaaaat caagtagaaa catttacagc cggctttgcc
     1381 caagtcgttc ctgaaaagca gctgatgaca gaagatctgt tgtttgatgc agcctcactg
     1441 acgaaagtcg ttgcaaccac ttcattagtt ttacgtctat tagaagatgg aaagatagaa
     1501 ctcgatcaac cgcttcataa atatcttcct gaatttgaaa atcagacaat cactttgcgt
     1561 catttactga cacatacttc tgatatcaaa acatggatac caaacagaga taagctatct
     1621 caggaagaat tgatcgccgc ttatttgaag ctagaaccag gtgagcaact aggaaaagaa
     1681 gtaaaatata cagatgccgg tacgattctt cttgggttga tgctagagga aatttaccaa
     1741 gaaactgcag tggaaatctt tcaagaacaa gtattggaac cattaggaat gatgaacagc
     1801 ttatttcttc cttttccttc caaaaaaatc gttcccacag aacaactgcc ttcgggtgag
     1861 gttttgcgtg gacaaactca tgatccgaaa gcaagagttt tggctgaaca tgcaggtaac
     1921 gcgggtctct ttattgattt gaaagacagc attacttttg tccaaatgta tttgaacaag
     1981 ggaaaaatcc gtaatggtcg ttttttagaa gaagagacga ttgattcttt actgcaagat
     2041 caaacaccga aaaaaaatgg ctatcgttct ttaggatggg acttaaaaaa ggatgtagaa
     2101 gacggtcatg cgattctttt tcatactggt tatacaggta cgtttttctt gattgatata
     2161 aaaacacaag aagcgttgat atttttgtca aatcgcgtac atcctgtcga tcatcgtgcg
     2221 gagtatctca tatgtcgaga tgaaattgtt gaaaactttt tgaaagagaa agcggtatta
     2281 ccgaaataat gctataatag ctatgaaaaa tgagaggagt ttgaattatg caggaaccta
     2341 tgtttttaaa accagttttt caagaaaaaa tttggggagg aagtcgttta cgttcagtct
     2401 ttggttttga tatccctaac gataaaatcg gagaagattg ggcgatcagt gcgcatcctc
     2461 atggtgtcag tgtagtagaa aatggcgaat tcaaagggaa aagattagat gaactatggt
     2521 cagaacacaa ggaattgttt ggccatcctt cagaaccagt gtttccatta ttgatcaaga
     2581 tcttagacgc agaagatgat ctatctgtac aagtacatcc agatgatgcc tatgggatga
     2641 aacatgaagg tgaattagga aaaactgaat gctggtatat catcgatgct gaacctggtg
     2701 cagaaatcat ctatggtcat catgcaaaga caagagaaga actagctgag atgatcaaag
     2761 atgggcgctg ggatgacctt ttgaagaaag taccagttaa aaaaggtgat ttcttttatg
     2821 taccaagcgg aacgatccat gctattggta aaggaatcat gattttagaa acacagcaaa
     2881 gttctgacac gacttaccgc gtgtatgact acgacagaaa agatgcgcaa ggacaaacaa
     2941 gagagctgca tatccaacaa tcgatcgatg taacaactgt ccctgcgaaa acaccagaat
     3001 tacaaatcaa agaagtccga aaagggaatt catcgatcgt cacttattta gaaactgagt
     3061 ttttcaatgt atatgagtgg gatattaaag gaatcacatc tttcaaaaaa caagcccctt
     3121 atacattgat gacggtaatc gaaggagcag gagaccttgt cgttgacgga aaaacatatc
     3181 cattagaaaa aggaacaagc tgtattattc caaatggcgt ttctgagtgg gcggtccaag
     3241 gtgaactagc aatcattgcg actgaaccag gcaaatagtt cccagatagc tgaaagcagc
     3301 agacggtttt tcgtttgctg ctttttctac ataaaatccg tccatcaata aatagaagta
     3361 tggtgaaaaa gatggaatac aaatggaaag caaacaacag cagtcgcgtg tctatgaaac
     3421 aactgagaaa agctggacat cacttaaatg aaatgatcat gccaaatcta agtctgttta
     3481 tcgcttgggg aatcttgtca ttagcaacaa aaggtgtgtc tggcgatttg cacaccacat
     3541 tagaagaggt atgccattgg atgatccagc tgcttttacc catcttgatc agttattcag
     3601 gcgggaaatc ggtaggggga caaaaagggg cagtcgccgg tgcaattgct tctttagggt
     3661 tgatcgctcg ttccaatgct ccacaaatcg taggagcgat gattatcggg ccgcttgccg
     3721 gcatcgtata tgaacaattt gtttctagat tccacgtgaa attcaaggct ggatatgaaa
     3781 tgcttgtcag caacctacta gtaggactga ctggtagcgt tatttgtata attggaatcg
     3841 tttttattga accagtttta aatagtgttc acttgttgct cgcctccgtt gtgtcttggc
     3901 tagtcacaat gaatctgtta ccacttgtca gtctgatctt agaacctcta aaagtactat
     3961 ttttcaataa tgcaatcaat catggtgtat tgacaccttt aggaatcgaa ttctcacaaa
     4021 cagtcgggta ctcttcctta tttttgatgg aggcaaatcc tggacctggt ttagggatct
     4081 tactttctat cctttgtttt gcagaaaaac aggaaaaagt aaatgcgagt ggagcattga
     4141 tgatccatgc gattggcggg attcacgaaa tctattttcc atttgtcttg ctgcgaccgt
     4201 atctattttt agctgtaatg gctggtgggg caagcggcac gttgatattt caatggttag
     4261 atacgggatt agctgcacct gtatcgcctg ggtcactact cgtgattttt gccaatgcgc
     4321 cttatccaca actagctggg gtaagtatag ggatagcaat cagtacattt gtgaccttca
     4381 tttgtgcagc gatactattg aaattaccaa ataaagaaaa agagaacaaa acggtcaaag
     4441 aaaggggaga aatcttggaa ccatctatca ttcattccgt catttttgct tgcgactcag
     4501 gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa gaaattagca gcagaaaaaa
     4561 tcgatctttc tgtcggatac agctctatct atgaattgaa taaccaagta aatcagctgg
     4621 ttatcgtgca acatgaacta gcgggtcttg caagagaaaa agtacctcaa agtcaaatct
     4681 atgtactgga tcactttttg gaaacagata tcttgctgtc acgaataaaa gaagcgatcc
     4741 aatttccaaa gcgggaagaa aaaaaagaga tagcagatat cacagaacaa aaaaagtcgc
     4801 ctgctaatat tgtatttttg tataaaaaca acagacgcgg ctcacaaact atgggcatga
     4861 cgattttaag tcaattagca aaaaaagcga aaaaacagtt tgttatctca aaagaatcaa
     4921 tagaaaaaat caaaatccaa gaagaaacga tttatatcgc acaaagagaa ctagctgtaa
     4981 ctgaacagct agaagaaaag atccctcggc tagttctagt tgaacattgg gtagctacga
     5041 atgaatacga aaaactcgtg aagggagaaa tataagatgt atttatcgcc cagagaaaaa
     5101 caattattag cagaatttct cagtagcccg tcacctgtat ctatccaaaa aatgatgaat
     5161 ttattgaagg tcagtaaacg gactatttac cgcgaattag ataatcttga agtctcattg
     5221 aaatcggtaa atgcttcttt gaaaaaagca gctagaggct catttgtcat tgaggcatca
     5281 gaagaagtat tagagcaatt aaaagaaaat acagatggga aaagtaatga cttatccact
     5341 gctaaacgac aacacgcgat tttagccgag ttgttactga cgacagagcc gttgagtctt
     5401 gctcatttca tggaatcata tcgcatcagt aataccactt tttatgcaga tatcaaacag
     5461 ttggaagaaa gtattcgcca actgcctttg gaaatcattc ggaaccaggg atacgagatc
     5521 gaaggaccgg aaaaataccg ccggcttctg acagcgaatg tattagaatt agaaatcaat
     5581 gaatatgaac tttttcatag tatttctttc gatgcatcac tgaattattt ttttcgattc
     5641 gttgatccgc aacatctatc tcttgcacgc aaagtaatcg gggaagaatt gatccaaaaa
     5701 aagaccaatc taagtgatag aaagcttgaa catttggttt tgatgctgac attgacgatg
     5761 gagcgtgtga ctaaaaatca tttactgaca aatgagacat ataatagtct ggcaaacaaa
     5821 gaattactac agacagctaa acgattgttt tcacggatcg cttctgagac aaaacaactt
     5881 tatccagtaa atgagatcgt cttttttgca ggattattaa atgacttcac gaactcattt
     5941 gatcagaatt tctttgaaga gacgttcgat acacagcttg cttatttagt aaaacaactg
     6001 attcaagaag taagtgaaga gacgaatgtc catttttatg aagatgaaac tttgtacaaa
     6061 atgctgttga ctcatttatc tggtgtgttt tcaagagccg ttttacaaga agagacactg
     6121 accaatccga ttttagaaaa aatcatggtt caatacgagg aagttgcgaa tgcaatccgt
     6181 aaagcagtag agcggatttt tcaagaaaaa aaattatccg aagaagagat cgcttatatg
     6241 gtccttcatt ttgcgaattc attggaacga agtccgaaag aaagcaatat cagtattgca
     6301 ggattttcgc caagtggatt agcttctact agcatgttgg aactacgatt gaaaaggtat
     6361 tttcccttca tccaacaaat tcatttctat cggattgctg atttaaaaaa aatcgattta
     6421 caagaagaat atgatgttgt tatttctaca tctattttac caggatacgg tggaaaatat
     6481 attttagtct ccccgctact gctagaagat gaagtcaagc agctaaaaga agctttttcg
     6541 catattgaga agaaaaaaag gcaaaaaatt acgtataaca gcaataacga gtggatgata
     6601 gaagatactt atgaggaaac aatgcatttc atggatcaaa tcaatcaatt actcggtcgt
     6661 ttttttatcc aagaactgga taatcctgag acgatttccg aaacgattcg cagcttattt
     6721 ccttattttc ctgaaggact agtaacggat gaagaggtgg ttcatcgccg gctgcttaag
     6781 cgatatgaac aagcacctat cggtattcct catacaaata tggggttatt ccatacatcc
     6841 agctcattga tttctaaacc gatcttttgt attttcaatt tgaaacatcc gttgaccatt
     6901 gaagggatgg atagacagcc agtcgatttg tcccgtatgt tagttatgct ggcgccttcg
     6961 ccaatccaag aaaaagacgg gattctttta ggaaagatca gtggagcaat catcatgaat
     7021 gacttgaaca ccgagatctt ccgctcagga aataaagaaa tcgtttatca gttgttagcg
     7081 aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag atgatacagc taccagcaga
     7141 aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa gcgttgaccg agatcggcca
     7201 gtggatgacc caacaagggg gaatcaaacc ggcatatatc caatcaatga tcgaaagaca
     7261 agaaaaagcg agtgtctata tcgggaattt cgtagccttg ccacatggga ctgacaaaga
     7321 tgaagaagta gtagaagaag gaatctattt agtccaagtg cctgatggtg tggactttgg
     7381 aacaaaagag gaaccaaaaa tcgcgacact cttatttgta gtggctttaa aaaaagaaca
     7441 tcagttagcg gtgctccaag atttagcctt tttctgttca gacatcgacc aagtaatggc
     7501 gctcagtgat gcgaaaacca ttgaagaagt ccaaatgatt tttgaacatg gacatatata
     7561 accaatgttc acagtaaaaa aagctgcctt gcatcaagta ataacttcaa ccaagataaa
     7621 aataagagga gctatttttg ttgggaggtt cttattactg cggatgcaag cttttgaaaa
     7681 ctatttattc tagaaaaccg tttatttgaa ataggggacg gtgacaaggt ttttgtcacc
     7741 gcccctatta tttttgtgtt ttaatagctc ttttttatat agatagtgcc catacctatg
     7801 aataataaaa cgatactcca tataccaagt gtcaagatac cattcatgaa agtaacctgt
     7861 tctgaagcta gtggcgagaa aatatctcct cctatgatca cattttgaaa atcaacataa
     7921 gaagttggca agtaatgact gattgattct atccatccaa tcgaagaaaa caagcgatac
     7981 tctgctaaga aaagacaaag aagtgtacct aaaagattga ctagagaatg ttcaaataaa
     8041 gcagatagaa gaaaactgat tgtacttaaa aagagcaccc acaacaacag gaacagaaag
     8101 ctctggaaaa gatactcata catcggaagg attcgtatct cctgaccaat gatcgttcct
     8161 acaggataac ggaagtctcc caaaccattt ttgattatta ctagtataga gacgatcaaa
     8221 aatggcatca cgagacttag catagctgca ccataataaa tggtaaaacg gatgctatgt
     8281 ttctttacca tactatccgg taatagattg actagagaga ttacctgctt tcttttttca
     8341 taagagatga tggaagaaag aaacaaggcg gttatacata aaatcaaaaa actagaaatc
     8401 attcctgaaa aaatattttc gtagtaattt gctaaaggga gatgcgctgg caagtcaaaa
     8461 atagaatatc tttggatctg atgttctttc atataacgga gcaactcgac acgtttttgt
     8521 tgttcaatga ttggaattcc ttgtctttga ccagaaatca gtcgatccag tatatctttt
     8581 tcataatgat aagttgcttc gaccatttgc tggacattcc ctgtgttttt tgcatgtaag
     8641 attgcttcga tcagtttgtt gcttttctct aaactttcca caaattcttt ctcattctca
     8701 tcttctttca acgtttcaat caaacgttgc atctcttcat agttatgatt cagttcatcg
     8761 aattctgtat aagcaagttt atgatcaaaa aaaagtggga atgcaaagag cacgaccaac
     8821 aatagaggaa ataaaaagtt tactttgttt tttagtaaga gattgcgttc aaatgtaagt
     8881 aaagacatag ttacacctca tataatttac gataaatagc taaaatctct tccatatttg
     8941 aggattctgt gacgatttct ccatttttta gaaaacatgc tttagaacaa atcgattgga
     9001 tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc ctctttctgc cattgtctga
     9061 tgaggctatt caagatatca atacttcctg ggtctaatcc gttcaacggt tcatcgaata
     9121 acatgacaga tgaatcactg atcaagtaag cagcaagcaa gacatgttgt ttcattccta
     9181 gagacatttt tccaatcggc aaagtaaggt actcagtcat cttcaatcgt tggaaaacag
     9241 tttggcattc tactttgctc tgccaatagt atttgatcaa agtcaaataa tcaacagccg
     9301 atagatagtc ggataactga ttcgttgatt ctagaaaaaa gaaagaccgg cgataatctg
     9361 tataagcagt aggagagatg ttgtgaaggg acaattgatc ataagttgtt ggcagcagtt
     9421 gggcaatgcc attcagcaaa gttgttttgc caaatccgtt cggggcaatc aaagcagcta
     9481 cttctcctga ttctactaaa agattcaatt gtcgaatcac acgtttttta ccacgtttca
     9541 tgttcatgtt tttgatcagt aatgtcatag ataggcctcc aatataaaat ttaataacat
     9601 aatagaatgg aaaacatcta aattaaatgc taacaaaagt tattgtttgc atattataaa
     9661 aaaattaaaa taacagtttt atttaaaatt gaagttttct gataccgaaa tctttttatt
     9721 ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc aaagtacgac tatttctctt
     9781 tttagtaagc ggatacaatt agagtatcaa aaggagagag ggagagacaa aatggaacgg
     9841 acgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg cagtacttta
     9901 tcaagcatgg tcatgccaaa tattggagca ctgattgcct ggggtgtatt aacggctcta
     9961 tttatcccag atggttattt accaaatgag gctttggcta ccatggtaag cccaatgcta
    10021 acttatctga ttcctttatt gatcggttat actggcggaa aagtaattgc tggtgaccgc
    10081 ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg tcggaacaga tattccaatg
    10141 atgctaggcg cgatgatcat gggacctctt ggtggatatg ccatcaagaa atttgatcag
    10201 ctattccaaa aacggatcaa atccggtttt gaaatgctag tcaataattt ttcagcaggt
    10261 cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt tgtcgatgca
    10321 ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt gattccattg
    10381 acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat caatcatggg
    10441 attttgacac cattgggaac tgaacaagta gcagaggctg gaaaatctat tctattcttg
    10501 cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt gtttggcaaa
    10561 ggagcagcaa aatcttcagc accaggagcc atgatcattc actttttagg cgggatccat
    10621 gaaatctatt ttccttacgt tatgatgaaa ccgttattat tcttatcagt tataggcgga
    10681 ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc gccagcttct
    10741 ccagggtcga tcattgcgat tctagcgatg acaccgttca attcttacct tcctgttatt
    10801 ttgggcgtga ttgttgccgc agctgtttcg tttggtattt ctgcagtaat tttgaaagcg
    10861 gatgcgaaag atacaaatga agacttcgaa aaatctgttc aagagacaaa ggcagctaag
    10921 caagcttcca aaggtgccca aggtattcct caaggagcta cattctctgg cgtacaaaaa
    10981 atcatttttg cttgtgatgc aggaatggga tcaagtgcga tgggcgcttc gatcttgaga
    11041 aaaaaagtac aggaagcaga cttgcctcaa acggtgacca atcaagcaat cagccaactg
    11101 acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag agcaaaacaa
    11161 aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt tgaattcacc tcgatatgat
    11221 gaaatcattc aagaattaag cagcaagcag aaaacatcag tttctgaaat tgagaatgag
    11281 ccagcagaag agtttctttc agcaaacatc aaacaaatca aagagattct acttgtccac
    11341 gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc tcaaagatat attagacaag
    11401 aaccatttaa ctatcccgtt aagaaaagtc aatatccaag atttaaaaga tgaacctgat
    11461 tcactgatcg tagccaaaga aagtttagcg gaaaaagtac agatggctgc acctcaatcc
    11521 attcatttat cagtcaacag catggtcaat ccgcaaaaat acgaatcgat tgcacaatat
    11581 ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc gacatgatcg
    11641 agctcaatcg atcttttaaa accaaagaag aagcgatccg ctattgcggt cagatgcttg
    11701 tagaagcagg gtgcgttgat gaggaatata tcgatgccat ggtagataga gacaaaatgt
    11761 tatctgttta tatggggaat tttatcgcga ttcctcatgg aacagatgaa gcaaaaaaat
    11821 atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga tggtgtagat tttggtaccg
    11881 aagaagaaga aaagattgcg actgttttgt tcggtattgc aggagtcgga gatgagcatt
    11941 tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt gtccagctag
    12001 cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa ggagagagaa
    12061 aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt ggagaggttt
    12121 tggcagctaa tggatttgag attactttcg tagatgtgaa tcacacgatc attgatgctt
    12181 taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa aaaatcactg
    12241 taaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc caagaaatcg
    12301 ctgaagcaga tttagttact accgccattg gcccgaatat cttacctttt attgctgagt
    12361 tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag cacaacatct ttagatatca
    12421 ttgcctgtga aaatatgatt ggcggttcag catttctaga aaaagaagtg tataaatatc
    12481 ttccggaaac atcatttact gacaaatata tcggctttcc aaatgcagca gtcgatcgaa
    12541 tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtggaacct ttcaaagaat
    12601 gggtcattga cgatagccaa agaaaaaata aaaaaatcca attaaaaggg gttttatatg
    12661 tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact ggtcatgcga
    12721 ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca atgcaagatg
    12781 cgttagttgt ggcacaatta aaatcagttt tgcaggaaac aggaagtttg ctgattgcca
    12841 aatgggggtt tgatgcagag cagcatcatg cgtatattga aaaaatcatt catcgcttcc
    12901 aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg gacaccatta cgtaagttag
    12961 gttatcaaga aagatttaca cgtccagtga gagaactaca agaacataac ttgacttgcc
    13021 cacatttgac ggcaacaatg gggattattt ttaattacta tgatcctgaa gatgaacaaa
    13081 gccgtcaatt gcatgaaatg aagatccatg aaaacttgga acaattgatt caagaagtca
    13141 caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac cgttatgcca
    13201 agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgaaatttt
//
LOCUS       DACEDO010000074        13207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEDO010000074 DACEDO010000000
VERSION     DACEDO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.074"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWO52_002305"
     CDS             complement(<1..914)
                     /locus_tag="IWO52_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0356552.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWO52_002306"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWO52_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0356553.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWO52_002307"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWO52_002307"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0356554.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWO52_002308"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWO52_002308"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0356555.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWO52_002309"
     CDS             4440..5321
                     /locus_tag="IWO52_002309"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0356556.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWO52_002310"
     CDS             5318..6034
                     /locus_tag="IWO52_002310"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0356557.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWO52_002311"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWO52_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0356558.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWO52_002312"
     CDS             6751..7752
                     /locus_tag="IWO52_002312"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0356559.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWO52_002313"
     CDS             7749..9110
                     /locus_tag="IWO52_002313"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0356560.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWO52_002314"
     CDS             9070..10221
                     /locus_tag="IWO52_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0356561.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWO52_002315"
     CDS             10196..11365
                     /locus_tag="IWO52_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0356562.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWO52_002316"
     CDS             11540..12919
                     /locus_tag="IWO52_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0356563.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         4400 a         2300 c         2873 g         3634 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaag
//
LOCUS       DACEDO010000075        12926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEDO010000075 DACEDO010000000
VERSION     DACEDO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.075"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..292)
                     /locus_tag="IWO52_002317"
     CDS             complement(<1..292)
                     /locus_tag="IWO52_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0356564.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFE"
     gene            complement(289..1230)
                     /locus_tag="IWO52_002318"
     CDS             complement(289..1230)
                     /locus_tag="IWO52_002318"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0356565.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(1407..1994)
                     /locus_tag="IWO52_002319"
     CDS             complement(1407..1994)
                     /locus_tag="IWO52_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0356566.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(2215..3528)
                     /gene="obgE"
                     /locus_tag="IWO52_002320"
     CDS             complement(2215..3528)
                     /gene="obgE"
                     /locus_tag="IWO52_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0356567.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            3816..4826
                     /gene="gap"
                     /locus_tag="IWO52_002321"
     CDS             3816..4826
                     /gene="gap"
                     /locus_tag="IWO52_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0356568.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(4911..5846)
                     /locus_tag="IWO52_002322"
     CDS             complement(4911..5846)
                     /locus_tag="IWO52_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0356569.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            6032..6991
                     /locus_tag="IWO52_002323"
     CDS             6032..6991
                     /locus_tag="IWO52_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0356570.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(7077..8171)
                     /locus_tag="IWO52_002324"
     CDS             complement(7077..8171)
                     /locus_tag="IWO52_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0356571.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            8360..9121
                     /locus_tag="IWO52_002325"
     CDS             8360..9121
                     /locus_tag="IWO52_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0356572.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(9236..9676)
                     /gene="fabZ"
                     /locus_tag="IWO52_002326"
     CDS             complement(9236..9676)
                     /gene="fabZ"
                     /locus_tag="IWO52_002326"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0356573.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            9896..10651
                     /gene="fabI"
                     /locus_tag="IWO52_002327"
     CDS             9896..10651
                     /gene="fabI"
                     /locus_tag="IWO52_002327"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0356574.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(10761..11774)
                     /locus_tag="IWO52_002328"
     CDS             complement(10761..11774)
                     /locus_tag="IWO52_002328"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0356575.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            11939..12859
                     /locus_tag="IWO52_002329"
     CDS             11939..12859
                     /locus_tag="IWO52_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0356576.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         3823 a         2743 c         2267 g         4093 t
ORIGIN      
        1 cttcgaaaat cccaaaaccc gcattgttta ccagaatatc gacgcgcccg acttcttcca
       61 tcaccttttc atataattcg tccacgcttt caggattgct gacgtctacc ttgaaagcat
      121 aagcctcttt gccgctcaag cgtctgcact cgtctctgac accttctaca aatgccattc
      181 ttcgtgcgca acttataacg attgctcctt gctttgctgc ttcataacag atttgtgcgc
      241 ccagaccgcc tgaactacct gttactaaaa caactttatc ttctaaattc atgcctcttc
      301 ctcctcgcga aatggaatct caatgctatc aaaatctttc ataatcgtcg tgttttcaaa
      361 cacttcttgt gcttcttttt caagctctaa agctgctttt cctaaatatc tcgcactgat
      421 atgagtaagt agcagccgtt ttgcctgcgc ttcttttgca acttcagcag cttgatgagt
      481 cgtggaatgg aaatagttgt gcgccattct tgcttcatct ttattaaatg tgctttcatg
      541 taccagaata tcgctatttt ccgcaagcac aacggaatta tgtgtttttc tcgtatcgcc
      601 taaaatagtc acgattcgtc cttttttatc tggacctacg aaatcttttc cattgatcgt
      661 ccgcccatct tctagttgga tcgtttcacc ttgcttcagt ttgccgtaca gtggtccagc
      721 tggaatacct aattctttta gacgatccac ctgcaattct cctttatgat catgttcaac
      781 cacacgataa ccaaaactgt cgatcccatg atttagtttt tttgtataaa cagaaaattg
      841 ctgatcagta aaaatcgggg ctgtttcatt tagctcaatg aatttcagcg gataacttaa
      901 acgcgtttgc gaaataccta aagagacttt tatgtattcc tctatccctt ttggcccgta
      961 aatctctaat ggcgtatccc caccttgaaa tgaccggctg ctgatgagcc cgggaagtcc
     1021 aaaaatatga tcgccgtgta aatgtgtaat gaaaatttta ccgatttttc tcggacgaat
     1081 agtcgttcgc aaaatctgca tttgcgtgcc ttcgccacag tcgaacaacc aaacttcatt
     1141 gcgctcgtct aaaagtttca atgctatact tgtcacattt ctatgttttg caggaacacc
     1201 tgcacctgtt cctaaaaatt gtaattccat aatctacctg actttcttta agttcctctt
     1261 taattttagg gtaaaaagaa gggataatca accgtaagtc gagaatgggt tcaaattaaa
     1321 aataacgttc taaagcgata gcgttttctt tgaaaattac tataaaaaca gcagcgaacc
     1381 cgtttgagat cctctgctgt tttttattaa tttgaagatc cagaactttc attgtcgttg
     1441 gaagtttgcg gatgtttgtc cttccaagtc ttttgtgcag cttctttcgc atgattttca
     1501 gcttctttcg catcatgcac atctttttcg acatcttcat aatccaagcg agtaatttct
     1561 ccgcctaatt ccatcattac caattgattc agtgggcgat tgtcctcttc atcagcaatc
     1621 aagatcagtc ctgtagctcc ttcaccgatt ttatttccta caaagtcgaa cacggtctgg
     1681 gcatctttta tttctttggc atcttgtgtc gcaccgatca ttcctccggc aaaccatcct
     1741 aataaaatcc ctagcggacc acctaataca ccaaccaaca ttccgattaa tccacccgta
     1801 gaagatttgt tattaccggt aaagtcgata aaatcttcta ttttgaattg atgtgtccca
     1861 ttaggatcat ggctgacaac tgccatctgt tctcctttaa tcgaacgaac agcatgtaac
     1921 tttttgattt ctgaaaatgc ctgatatgcc tggctttcta cttgaaaatt taaaatgatt
     1981 actcgtttgc tcattaaaaa cgcccctttc ataccttctt gttatttatt ttacctttaa
     2041 ttgaaaaata agaaaagaaa agcactcttt ttttccaata aaatagggct gtgacaaaag
     2101 tcgtatcaca gctccttttt ccgaataaac ggcgttcttg aagctgatca cttctgtcac
     2161 aacctccctt aaatctgaat ctatattttt tggtatttgt ttttatttgc ttgcctattc
     2221 gacaaattca aattcaaact caccgatacg tacaaggtcg ccatctttcg caccgcgtgc
     2281 ccgaagagct tcatccacac ccataccacg taattgacga gcaaaacgca tcactgtttc
     2341 atcatgatcg aaattcgtca tttggaataa tttttcgatc ttctcaccag ataagatcca
     2401 cgttgcatct gaatcacgat cgatttggaa ctcaggacct tctggattga atccatagtg
     2461 tacagtttct tcttccagtt cttcttcgta caatgggaat tctggcgtta cttctaacaa
     2521 atctgctgta gcattcaata acgcgtccaa tccttgtcga gtcactccag aaattggaaa
     2581 tactgggatg tcatcagcaa attcatcttc tttttccttg ttcagctgtt ctttgaattt
     2641 cactagattt tccggtgcat ccggcatatc cattttgttt gccacgatga tttgcggacg
     2701 ctctagcaag cgtaagttat aagtagataa ttctttgttg atggcaagat agtcttcata
     2761 aggatctcgt ccttccatcc cactcatatc gatcacatgt aaaattactc gtgttctttc
     2821 gatatgacga aggaattgtg tacccagccc aacaccttgc gaagctcctt caatcaagcc
     2881 tggcaagtct gctgcggcaa aacttctgcc atcacttgta gtaaccattc ctagattagg
     2941 tacgagcgtc gtgaaatgat aagcgccgat ttttggacga gcagatgaaa taacggatag
     3001 taaagtcgat ttcccgacag atggaaaacc tacaagtcca acgtctgcca atactttcaa
     3061 ttctagttcg atttttcgct cttgtcctgg ttcaccgttt tcagcaattt ccggtgcagg
     3121 attccgagga gaggcaaaac ggatattccc tcgtccaccg cgtccacctt ttgccacaac
     3181 tagtgtttga ccattttcaa ttaaatctcc taaaagagca ccagtctccg catctcggac
     3241 agtggttcct tgtggtactt taacataggt atcttctgaa ccacgaccgt gcatcccttt
     3301 actcatccca ttttcacctg gctgcgcttt gaaatggcga ttgaaccgaa aatccatcaa
     3361 tgtacgtaac ccttcatcga cgatcaagat gacatcgccg ccacgaccac cgtctccgcc
     3421 agccggtccg ccgtcaggaa cgtatttttc gcgacgaaat gcaaccattc cgtctccgcc
     3481 cttgccagct ttcacatcga tcgttacttg atctaaaaac atggacataa ttaagtcctc
     3541 ctattattca tttatttatt tgactgcctg aatttccaca ataaacaaaa gtcttactta
     3601 ttttatcggt gaaatctgat tttgtctatc atttcagcaa aaagattgtt taccttcttc
     3661 tttttttatc atttggtaag gtttccaacc ttttttctgc taccaagtat aattttttga
     3721 caaaatattc tttgcgtagc agaataacag tgtattaatt gtattaattg aggatgccgt
     3781 ttcacagcac ccctaataaa ggaggaactt ttgatatgac agtaaaagta ggtattaacg
     3841 gatttgggcg tattggccgt ctggctttcc gccgcatcaa agaagtctca gatgatattg
     3901 aagtagtagc aatcaacgac ttaacaagtc caactatgtt agcacaatta ttgcaatttg
     3961 actctactca tggtacttat cctggcacag ttactgcaac agaagactca atcgtagtgg
     4021 atggcgaagc aactcgtgtt tatgcagaac ccgacgcaag caaaatccct tgggcaaaag
     4081 aaaacggtgt agatatcgta ctagaatgta ctggttttta tacatctgaa gaaaaagcaa
     4141 aagcgcattt agatgctggt gtcaaacgag tagttatttc tgccccagct ggtgctatga
     4201 aaaccatcgt ctataacgtc aatgatgata cattagatga aaacgacaaa atcatctcag
     4261 caggttcatg tacaacaaac tgtttagcac ctatggctta tttcttaaat aatgaatttg
     4321 ggatcgaagt tggtacaatg acaacagttc atgcctacac ttccacacaa atgctcttag
     4381 atggacctgt aaaaggcggt aatttgcgtg ctgctcgttc agctgctgac aatactatcc
     4441 cacattctac tggtgctgcg aaagcaatcg gcttagttat ccctgagcta caaggaaaat
     4501 tacaaggaca cgcacaacgt gttccagtcg ttgatggttc attaactgaa ttggtttcag
     4561 tattgaaaac aaaagtaact gctgatcaag taaacgaagc aatcaaaaaa cacacagttg
     4621 acaatccttc attcgggtat gatgaccgtc aaatcgtttc tagcgatgtc attggaacaa
     4681 ctcaaggttc gatctttgac ccaacgcaaa cagaagtaac aactgctggt gatttccaac
     4741 ttgttaaaac tgttgcatgg tatgacaatg agtatggatt tacttgccaa atgattcgct
     4801 tactagaaaa attcgctaac ttgtaatttg atagatcaat cgataaaaga agcctttgac
     4861 tggatccagt caaaggcttc tttcttttca tcccctactt ttaaactcgt tcaatgaaca
     4921 tattggctta aaaattgttg gactacttgc tgatattttt caggatctgt cttgtatgct
     4981 tccgcatgct cggctccttt tacaacatac ttttcttttg gtcccttcgt tgcacgatag
     5041 accttatcta acatttcaaa tggtacaaat gtatcttcgt ctccatgtat aaagagcatt
     5101 gggcgctggt ttttcttcaa ttgttccact gcatttgctt caccgaaaaa gtagccagca
     5161 cggacttttg tcactagact cgttacttgc ataagcggaa atgctggaag actaaacata
     5221 tcgtctagct gataagcaag ctcatcatga acggaagaat aaccgcaatc ttcaatgatt
     5281 gctttcacat tatctggtag tttttctccg ctagtcatca taactgttgc tgctcccatg
     5341 ctgacaccgt atagaacgat ttcttgcgat tttccattgt tttctagcac tttgtttatc
     5401 cactgtacat agtctttacg ctctggccat ccaaatccaa tgtaatcccc ttcgcttttt
     5461 ccatgtcctc ttgcatcagg aacaaggaca ttgtaaccca tatcatgaaa catctttgcg
     5521 taattggtca tcgtttcagc gtttcccata tagccatgag ccacaagagc tgttttttca
     5581 cttttcgttt cagcaggaag ataaatcgct gataatttca gaccgtcttt tgaagtgatg
     5641 gtccaatcgc ttcggttttc ttttgaacta aaccatttat gtgcatccgt ttctttggat
     5701 gtctgtgttg taccaggtgt atcttcgtct aaaaagtctt tctcagaagg cacaacagca
     5761 taattataaa aataattccc tgcaaacact aacgcaacaa tcagagctaa agcgacgcct
     5821 aaaaggattt tcatccaaag tttcataaat catccatctc atttctcttt tttcatataa
     5881 ccttgaatta gtataatcaa agaatctcta ctatgcaatg ttcattttta taacccgcga
     5941 attgtaaaat taagttagaa aaataaaaag gcatttatgg tacactcaaa ttgtatttcc
     6001 gacgaaagaa aacaaaggag tgtaaccata aatgacctac aaacatctta ccatagacga
     6061 actgacaatg atagaatcat attatcttca acataataag ccggttgaaa tcgctaaccg
     6121 aatgggacgt gctatacaaa ctatttataa tgtagtcaat aagttcaagc aaggcaagac
     6181 tgctcttgat tattggcacc agtataaaga aaataagaaa aaatgtggta gaaaagtcat
     6241 tcaattacct gctcatgaag tagattacat taaagagaaa gtcactcttg gttggacgcc
     6301 tgacgtcatt atcgggcgaa aagaaaggtc tgtttcatgc ggtatgagaa cactttatcg
     6361 tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta aaagaaaacc
     6421 caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc atgatcgacc
     6481 tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata cgatcgttgg
     6541 cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag tcattatcac
     6601 gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc aatggttttc
     6661 tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag aattttctaa
     6721 ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc ctggaacacc
     6781 ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg gactgccgaa
     6841 atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca atcaacgtaa
     6901 tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt tgagctatgt
     6961 acaagaagca ttttattcta gcttaatttg acaaatcata acctataaaa aaacaaaata
     7021 aaaaagcaga aataactggc tatttctagc ggattatttc tgcaataact aattatctat
     7081 tttttatttt caattaattt aaagcttgac catggtttca aaaagtcaag gaggaataat
     7141 tcgtcatcgg agattcttcc tacaacattg acacgtccgt cgtttttcat atccttcaag
     7201 gcgatttgtg tttctccttt gtattgaccg tacccttcat tgtcaatcag tacatcccca
     7261 tgatgcatat ctcgcgtatt atgaggcggg aaatctttat ctttgtagta tacacgagtc
     7321 atcgttgaac gaaggatata ttctgagcga tcccctcgat aactatgaag gttatcaaac
     7381 aagcagattc gttcgttttc tgtaatacct tcttccgtat ctactttcaa tgttggataa
     7441 tccgcgttaa atgcttctgc catttctttc aattcttctt cacttgcgta cgcatttcca
     7501 atagaaatat catcaatcaa tcctgtaagg attaaatgct tcacttgtgt cgcaatttca
     7561 agatctcggt ggtcttccag cgtgcacaag ccatcttgcg taggccaagg gccaaactca
     7621 gcagattgcg aattgacgaa tgccatcgta ttcaaattat attttctgaa tttttctgaa
     7681 caaaaaacaa agtgttcgta tcctaaacca gagtatcgat gaggataaaa attatgagaa
     7741 cctaataaat tatccgtgtt tggcgaataa ctcatgatgt tgtcaacgta attcgtgccc
     7801 gaactcatat tgatctcgat tttgatacca aaaggattgc gcgtcatttt tgcttcttct
     7861 gctcctgtga atccgatatc taagcggacc ccgtaggcac ccatcttatg gaaaaaagat
     7921 aagtcatcgt aagaaatctc caactgttca aataacgctg gattgatatc aaccattact
     7981 tccatgccta ggctattcgc gtaatcaact acttctttaa attctgaaag aactttttct
     8041 ttatcatcat tgatctgcag caaactggta aaaacccgtt tatacccata cttatgagct
     8101 aaatccaagt acgctttgtc cttttcaaat gttgaccgtt ctggataaat cgagattcct
     8161 aattttccca tattaccatt tcctcctgca tcttttcgtt tttgtatcca cttacattat
     8221 agcaaacaca aagtaaatgg tctacacctt ttctttttct tttaaaataa atgataaatt
     8281 ccaataattt aagtaggtat tttccctcta ttttgttatc attaaagata acaaaatagt
     8341 aaattggaaa ggaacagtta tgaagaaaaa taaagttatt ttaagtatta gtttagcagc
     8401 agcattgctg tttgctgctg gttgttctgc aaacaacacg acttcttcta gttcgtctac
     8461 agaaagttca aaaagtacat ctactgcgga aagcacgact gccagtagcg tcgatttgaa
     8521 cgcattggaa ttaccacaat tgacgaaaga tgtcgccgaa aatgaagatc tggtcgaaat
     8581 ggtgacgaat aaaggcacaa tcaaaatcaa attatttccc gaacacgcac caaaagcggt
     8641 tgaaaacttt atgacacatg caaaagacgg ttactacaac ggactgactt tccatcgcgt
     8701 catcaaagat ttcatgatcc aaggtggcga tcctaaaggc gatggcactg gaggggaaag
     8761 catttgggga gaaggatttg aaacagagat caacaaccac ctttataata taagaggtgc
     8821 gttatctatg gctcgctcac aagatcctaa cagtaacggc agccagttct ttatcgttca
     8881 aaatacagat gatatgcatg atggtctttt aaaagacgac tatcctcaag caatcatcga
     8941 cgcttataaa aacggcggtt atccttcact agatggccat tatactgtct tcggccaagt
     9001 gattgaagga atggatgtcg tggatgctat tgcaaatatc gaaacagata gttcagacaa
     9061 accaaaagaa gaagtaaaaa tcgataaaat caatatactg caagaagcaa aacaaaaata
     9121 aaaaaataac gtctgaatcc tgcatagatg acaacgtatg ctaggattca ggcgtcttct
     9181 ttttacataa aaagcaagta agcttccttt tgttttgcta tatcaggttt tatagtcaag
     9241 cattttggtt gacgatgaac gtgaactcgc actcacagac ttttttatca tctacatatg
     9301 ctgcagataa cgctgtaccg actgatccct tcattttggt gatttcaaaa tgacatttca
     9361 gcacatctcc tggaacaact ttttgacgga atttggcctt attgatccca ccgatataag
     9421 ctctttttcc tagaaactct tctgatttta ggataagtat cgaacctgct tgtgccagtg
     9481 tttctagaat caacgcaccc ggcatcactg gattgtcagg aaagtgacct tggaaaaaat
     9541 cttcattgat tgttacattt tttgtcgcaa cgatcttttt tcctggttct aaagtatcta
     9601 cataatcaat ataacaaata ggataacgat taggaatcaa ttccattact tcagtagcag
     9661 ataatacgta agacataatt aaactccttc tttcaggcaa ttgccatttt cattactttg
     9721 actatcaaag tatcatattc ctaaatattt agcaatcttt ttttaattta agaaaaaata
     9781 gtttgacaat caaaacattt tgactaataa ttaagaaatc aagaagttat tttagctatt
     9841 attctctatt tttttacgac tatcaaattt ttatcagaaa caaaggagag tcattatgtc
     9901 atttctagaa ggaaaaaaaa tcgtcattat gggcgtcgct aacaaaaaaa gcattgcttg
     9961 gggttgtgct gaagcaatgt tgaaacaagg ggcagaaatt atctatactt accaaaatga
    10021 acgaatgaaa aaatcattag tcaaattggt cggcgaagaa gcattcactg tagaatgtga
    10081 tgtttctagc gacgaaagca tcgaaaaagc aatgaatgcc atcggtgaat atgctgggga
    10141 gatccacggc cttgtccatg cagttgctta cgccaacaaa gaagaattgt ccggaaatgt
    10201 gtctgatatt tccagagacg gctatcaatt agctcaagat atcagcagct actctttaat
    10261 tgctgtatca aaatacgcac aaccgtttct tgcaccaaac agcgggatcg ttagcatgac
    10321 ttatctaggt tcagaacgcg caatcccgaa ttataacatg atgggtattg cgaaagcttc
    10381 tcttgaagct gctatccgtt atttggcagc tgagttctcg cctaaaggaa ttcgggtcaa
    10441 tggaatctca gctggggcaa tcaaaacatt agctgtcaca ggtgttaaag actatcaaaa
    10501 actgatccag ttatctgaat ctcgtacgcc tgatcagcaa ggtgtgacga tcgaagaagt
    10561 cggtaatgct tgtgctttct taatcagccc actttccagc ggaatcatcg gtgatattat
    10621 ctacgttgac aaaggtgtcc atctttcata aacttttgtt gcattcttta aagtaaaaca
    10681 tataaaaatc aggaaaaaac tttttaaagt ctcttcctga ttttttcttt ttacgttttt
    10741 ttcaactcat tactttgaaa ttattccacg acttcaatcc agccttctgg tgcttctatg
    10801 tctccaaatt gaatacctgt caattcatca tacaaccgtt ttgttactgg tcctacttcc
    10861 gtttcactat aaaatacatg aagattgtta ttgtgataaa tcccaccgat tggagaaata
    10921 acagccgctg taccacatgc accagcttct gcaaattgat ctagatcatc aatgaaaata
    10981 tctccttcaa ttgcttgcag tcctagtcgt tcttcagcta actgcaacaa ggaatatttc
    11041 gtgatacttg gtaagataga gggcgattgc ggagtgatga attttccgtc ttttgtaatt
    11101 ccaaagaaat ttgctgcacc tacttcttcg attttcgtat gtgtggctgg atcaagataa
    11161 acacaatctg agtaatccag ttcttttgct tctgttcctg gaagtaaact cgctgcgtaa
    11221 tttccgccga cttttgctgc accagtcccc ttaggagcag cacgatcgta atcagataca
    11281 ataaaattcg ttggttttag tccacctttg aaatacgctc ctactggcgt acagaagacg
    11341 acaaataaat attctggagc tggatggaca ccaatgtttt caccgacccc aatcagtaaa
    11401 gggcgcaagt ataatgtagc gcctgttcca tagggcggca caaaatcttc atttgctttt
    11461 accacttgct tgacagcatc aacgaacatc ttttcaggga ccattggcat cagcaatcgt
    11521 ctagcacttt gattcatccg tcgactattt tgttcaggtc gaaagagatt gaccttgccg
    11581 tcttttcgac gataagcttt tagaccttca aaacattgct gaccataatg aagcgctgga
    11641 gagccttcat gaatcgtcag gtaattatct tctgtcaatt ctccttcttc ccattgtccg
    11701 tctttccatt tggcaataaa acgccaagga gttttgatat aagaaaaacc tagattgttc
    11761 caatcgattt gcatatactt accaccttcg ttattttctc cacattttat cattatccat
    11821 attatttgtc attttatttt cagctttttt ccttcattat agttattttt gctatttcct
    11881 tttttgaaaa cttcttggta caatacagaa agaatcaaat tttagaaaag aggattttat
    11941 gttttcatta ttagcaactg ccggtacgac agactcttct ggcgagacat tgacagatca
    12001 agctgtcagc aaagtaaatg cgtttcagcg gttttgggac gggattgatt gggacgccat
    12061 tttggctgcc tttatccaaa aaagtttata tctgattttt ttgatcgtcc ttttttggat
    12121 tcttcaccgt gtgggaaaat atctgattga taagtctttt aatcagtatt ctaaaaaagc
    12181 cattctaaac gagagccgct taaagacttt acatagtctt ttaaatacta tttttcatta
    12241 tacccttggt ttctttttta tctatgcaat tttatctgct atcggtgtgc cgatcggatc
    12301 acttttggct ggtgccggaa ttgcaggtgt ggcaatcggt cttggtgccc aagggttcat
    12361 gagtgatgtt attaccggat tttttattat catggaacaa cagatggatg ttggggacta
    12421 catcaagcta gcaaacttaa cgattgaagg aaccgtcgta tctgttggta tccgtaccct
    12481 tcagctgaaa gcaacggatg gtactgtcca tttcatcccg aatcgaaaca tcaccacgat
    12541 cagcaatctc tctcgtgcaa atatgcaagt acttttagat attcggattg ttcctgaaga
    12601 aggatatgac aaaatctatg agatcattga tcgagtaaat cgtacattag ctgaaaaata
    12661 ccaagaagag ctccaaactg aaccttctat ctttggatta gtggatcttg gaaacagcaa
    12721 ttttgcgatt cgtacggtct gctacgcatt gaacggaaaa caatatgcct taaaagaaga
    12781 gttcttgagc tcctatgtca aagaattgac agcttctggt ttcacgattc cgaacagtcc
    12841 aattgctgtc ggaaaataat ttggacaaac gatgcaacga atctcactaa aaagagcgtg
    12901 ggacaaaagt aaagaacact tttgtc
//
LOCUS       DACEDO010000076        12904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEDO010000076 DACEDO010000000
VERSION     DACEDO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.076"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(260..979)
                     /locus_tag="IWO52_002330"
     CDS             complement(260..979)
                     /locus_tag="IWO52_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0356577.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="IWO52_002331"
     CDS             complement(1028..2674)
                     /locus_tag="IWO52_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0356578.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="IWO52_002332"
     CDS             2853..3443
                     /locus_tag="IWO52_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0356579.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="IWO52_002333"
     CDS             3535..5040
                     /locus_tag="IWO52_002333"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0356580.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IWO52_002334"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IWO52_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0356581.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="IWO52_002335"
     CDS             complement(6476..6994)
                     /locus_tag="IWO52_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356582.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="IWO52_002336"
     CDS             complement(7010..7336)
                     /locus_tag="IWO52_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0356583.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="IWO52_002337"
     CDS             complement(7349..9328)
                     /locus_tag="IWO52_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0356584.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="IWO52_002338"
     CDS             complement(9349..9663)
                     /locus_tag="IWO52_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0356585.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="IWO52_002339"
     CDS             9831..10124
                     /locus_tag="IWO52_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0356586.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="IWO52_002340"
     CDS             complement(10202..11314)
                     /locus_tag="IWO52_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0356587.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11701..11880
                     /locus_tag="IWO52_002341"
     CDS             11701..11880
                     /locus_tag="IWO52_002341"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356588.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="IWO52_002342"
     CDS             12074..12244
                     /locus_tag="IWO52_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0356589.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>12904)
                     /locus_tag="IWO52_002343"
     CDS             complement(12668..>12904)
                     /locus_tag="IWO52_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0356590.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4076 a         2590 c         2191 g         4047 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg
    12901 catc
//
LOCUS       DACEDO010000077        12738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEDO010000077 DACEDO010000000
VERSION     DACEDO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.077"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO52_002344"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO52_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0356591.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWO52_002345"
     CDS             complement(322..891)
                     /locus_tag="IWO52_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0356592.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWO52_002346"
     CDS             1077..1979
                     /locus_tag="IWO52_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0356593.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO52_002347"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO52_002347"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0356594.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO52_002348"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO52_002348"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0356595.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO52_002349"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO52_002349"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0356596.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWO52_002350"
     CDS             8547..8732
                     /locus_tag="IWO52_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0356597.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWO52_002351"
     CDS             complement(8794..9990)
                     /locus_tag="IWO52_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0356598.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWO52_002352"
     CDS             complement(10160..11596)
                     /locus_tag="IWO52_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0356599.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWO52_002353"
     CDS             11889..12698
                     /locus_tag="IWO52_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0356600.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattt
//
LOCUS       DACEDO010000078        12689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEDO010000078 DACEDO010000000
VERSION     DACEDO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.078"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWO52_002354"
     CDS             6..305
                     /locus_tag="IWO52_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0356601.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWO52_002355"
     CDS             378..1739
                     /locus_tag="IWO52_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0356602.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWO52_002356"
     CDS             1761..2021
                     /locus_tag="IWO52_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356603.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWO52_002357"
     CDS             2035..2787
                     /locus_tag="IWO52_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0356604.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWO52_002358"
     CDS             complement(2881..3486)
                     /locus_tag="IWO52_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0356605.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWO52_002359"
     CDS             complement(3539..4513)
                     /locus_tag="IWO52_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0356606.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWO52_002360"
     CDS             complement(4891..5670)
                     /locus_tag="IWO52_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0356607.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO52_002361"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO52_002361"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0356608.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWO52_002362"
     CDS             7161..8558
                     /locus_tag="IWO52_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0356609.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWO52_002363"
     CDS             8639..9079
                     /locus_tag="IWO52_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0356610.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWO52_002364"
     CDS             9227..9553
                     /locus_tag="IWO52_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0356611.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWO52_002365"
     CDS             9554..10303
                     /locus_tag="IWO52_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356612.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWO52_002366"
     CDS             complement(10383..10838)
                     /locus_tag="IWO52_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0356613.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWO52_002367"
     CDS             complement(11265..11540)
                     /locus_tag="IWO52_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356614.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWO52_002368"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWO52_002368"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0356615.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4060 a         2180 c         2590 g         3859 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaat
//
LOCUS       DACEDO010000079        12590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEDO010000079 DACEDO010000000
VERSION     DACEDO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.079"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..899
                     /locus_tag="IWO52_002369"
                     /pseudo
     CDS             47..899
                     /locus_tag="IWO52_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            996..2882
                     /locus_tag="IWO52_002370"
     CDS             996..2882
                     /locus_tag="IWO52_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0356616.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFETPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(2923..3825)
                     /locus_tag="IWO52_002371"
     CDS             complement(2923..3825)
                     /locus_tag="IWO52_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0356617.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            4055..5458
                     /locus_tag="IWO52_002372"
     CDS             4055..5458
                     /locus_tag="IWO52_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0356618.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            5458..6891
                     /gene="ascB"
                     /locus_tag="IWO52_002373"
     CDS             5458..6891
                     /gene="ascB"
                     /locus_tag="IWO52_002373"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0356619.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7036..7962
                     /gene="trxB"
                     /locus_tag="IWO52_002374"
     CDS             7036..7962
                     /gene="trxB"
                     /locus_tag="IWO52_002374"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0356620.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8176..9903
                     /locus_tag="IWO52_002375"
     CDS             8176..9903
                     /locus_tag="IWO52_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0356621.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            9976..10269
                     /locus_tag="IWO52_002376"
     CDS             9976..10269
                     /locus_tag="IWO52_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0356622.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(10364..11224)
                     /locus_tag="IWO52_002377"
     CDS             complement(10364..11224)
                     /locus_tag="IWO52_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0356623.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            11316..11744
                     /locus_tag="IWO52_002378"
     CDS             11316..11744
                     /locus_tag="IWO52_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0356624.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11906..12082
                     /locus_tag="IWO52_002379"
     CDS             11906..12082
                     /locus_tag="IWO52_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0356625.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12109..12555
                     /locus_tag="IWO52_002380"
     CDS             12109..12555
                     /locus_tag="IWO52_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0356626.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4119 a         2170 c         2775 g         3526 t
ORIGIN      
        1 tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc tgattgagaa
       61 agtactgaat aacaacgtcg taataaccag ggatgaaaga aatcaggaaa tgatcgtgat
      121 gggaaaaggt ttggcattca aaaagaaagt gggtgacccg atttttgact catgcatcga
      181 caaaaaattt cagttgacaa atgacgggct ttcacaacag ttccaagaat tggcacaagc
      241 gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa aagaggctct
      301 acaagtccag ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt atacggctat
      361 cgaacgtcac aaaatgggaa tatttgtacc aaatgtattg ctgtatgata tcaaacaact
      421 atttcctaaa gagtatgcca tcgggaaaaa aaacagtgaa gaaaatctcg gataaatacc
      481 acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc aatgcacaag
      541 gcattcaaag ccaacaagac aatacctacc tgttcactat aacaatacag gatattctaa
      601 atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat ttttttcgat
      661 tcgtcacaca cttacgtttt tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg
      721 gagaagtttc tgaggaattg tttcaggtta ttcaaaaaaa atataaggat acgtttggtt
      781 gcgttgagaa aatagcagac tttttagccg agacgttcaa ctaccagatg tccacggacg
      841 aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt tgtaaataaa
      901 taaaaaaagg aggataaatt aacaagaacg tatgaaataa gggtagtgac attcagttgg
      961 ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaataccaa gaattagcga
     1021 gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca
     1081 caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata
     1141 tggacggtat tgtaacggta atgcgagcgg gagggcagta tcaagttgtc atcgggaatc
     1201 atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg
     1261 tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc
     1321 aaccattcct tgctatccta gctgctgacg ggatgattcg tggactgaca gcattccttg
     1381 tcttcttagg cgcgtttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg
     1441 attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag
     1501 taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg
     1561 aagctttgag tggtgcagca gaagcaccac taacgaccat attttctggc actatttttg
     1621 aaacacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
     1681 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa
     1741 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
     1801 tgcctttagg ttttttactc gttggaccag tattcacatt cgcaactgat attttaatgg
     1861 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
     1921 tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
     1981 aattttctgt gaatggttgg caaaatatta tgacacctgt tgcagtagtg tcttttggac
     2041 aaactgcagc acttaccgca ctctacttta agcttagaaa tccaaaagac aaagcaattg
     2101 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
     2161 cattgccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
     2221 atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
     2281 ccaactatat tagaccagat ggtgatttaa cagatgtgat taatgtattg attgggatcg
     2341 ccattgcgat ggtggtatcc tttgcgctga cttttttctt ttggaaggag gaagcagatg
     2401 aaacagatga tagacagaaa agatcaggta aagaaattgt aaaaacaccg atacaaggac
     2461 aaatcgctcc actgaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag
     2521 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatgt
     2581 ccctgtttcc gacgaaacat gcaatcggga tggtttctga agcaggtctg gaattgctga
     2641 ttcatgtcgg attggatact gttcagttgg aagggaaata ttttgaaagc ttggtgcagc
     2701 aaggagaaag agtgaaacaa ggcgatctgt tgcttcgctt cgacaaaaag aaaatcgaag
     2761 aggaaggata cagtttagaa acacctgtga ttgtcatcaa ttacatggat tatttggata
     2821 ttttggaaaa tcaaggggaa acggtcgggc cagacgatgc acttatcacc gcactgacct
     2881 gaacggttag gtgaaagaat ggactaggct taactattct ttttagtcca cttctttatt
     2941 ttcaaaattg aaaggctctt tcatgatggc tgagctgata gggcgtttat caaatttttg
     3001 tcctacagaa attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata
     3061 taacacgtct agaagataac aaatcgatgt attgatcgta aaattaccaa ttttcgaata
     3121 cagtcgttcg cgtgtcgtaa gtcgcaaagc ggctgtactc attgaagata gcgttgaatc
     3181 accgatactg gtcaaagcta aaattggcac atgattttgt ttcaatattt ctgcggtacg
     3241 aaggatcaca ttgttttcac ccgtataaga aatcagaatc ccacacatat cacttgtgca
     3301 attgtacgct tcatagaacg attcaccaaa agtggtgaca atcgtgacat ccattttgat
     3361 gcgcttcatt ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaaaat
     3421 cacgattttt tttgcttgaa gcattagttg tttggctttt tgcagttggt catgatgaat
     3481 gagagaaagt gtatcttgaa tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt
     3541 cataataccg tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc
     3601 aagataggct gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat
     3661 tttttttgct acacggatga gtgtggacgg gtggacataa catcgctcag caatctcttg
     3721 gatggtcatt tcgccaagtg ctggagaatc gcttaagaga aactgaatca cttcattttc
     3781 tgaaggagaa aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc
     3841 ttgttttttt acgtaaatta taactttttt gaaaatgatt tgtaaacgga tttatgttat
     3901 atagcttaat aaagacattt tttgacaaga tatttacaaa aatccagaag aaaacactaa
     3961 cttttgtaaa tgttactgga aagaaacgaa tttctattgt ttgttttgtt tacaaaaaag
     4021 ataataaaag tgtaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac
     4081 gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga
     4141 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
     4201 gatcgtcagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaaaatcta
     4261 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
     4321 tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat
     4381 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtggt
     4441 tttggcaatc gttgaagaca tgaagcgttt atgtccaaat gcttggctga ttaatttcgc
     4501 gaacccaagc gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
     4561 cggattatgc aatgtgccag taatggcgat gatgacagaa ccaacaatgc ttggcaagaa
     4621 aaaagaagag ctgatctaca agtttgccgg tctaaatcac ttccattggc acaaagtagc
     4681 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagacaa
     4741 tggattgccg aaaaatatct ttgacatccc gtttttcaaa gaacaattaa aacaaatgaa
     4801 catgatccct tgtggctacc accgctatta ctaccgtgaa gaagaaatgc tggaacatgc
     4861 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
     4921 gctatttgaa ttatacaaag atccagaatt agaccataaa cctgaacaat tgcaaaaacg
     4981 aggtggcgct tattattcag acgctgcctg tgaaacgatt gcttcaatct atggaaataa
     5041 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgacc tgccggcaga
     5101 ttgtgtagta gaagtcactg cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg
     5161 ttctcttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
     5221 gatcgaagca gctgtgactg gtgattatgg gacagcttta caggcgttta cgatcaatcc
     5281 gcttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacacca
     5341 gcagtattta ccgcaattca aggaaacaat cagccggtta aaagcagaag gaatcgaagt
     5401 ccaagatgaa ctggcaagag aattatcaag caatacacat gtaatggagg aagtatgatg
     5461 ggattcagaa aagatttttt atggggcgga gctacagcag ccaatcaatg cgagggcggt
     5521 tatgatgaag gcggacgtgg gttagcgaac gtagacgttg tgccaatcgg agaagaccgt
     5581 tttcctgtca ttactggaaa aaagaaaatg tttgaatttg atgaaaatca tttttatcca
     5641 gcaaaaattg cgatcgatat gtatcatcat tataaggaag atattgcttt atttgcagaa
     5701 atggggttca agacttatcg gatgtccatt gcttggagtc gtatttttcc aatgggcgat
     5761 gaaaaagagc caaatgaatc tgggttgaaa ttttatgagg atgtctttaa agaatgtcaa
     5821 aagtacggga tcgagccgct tgtgacgatt acacattttg attgtccgat gcatttgatc
     5881 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgccga
     5941 gtcatcttca atcgctataa aggacttgtc aaatattggt tgacattcaa tgagatcaat
     6001 atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaagaagg agaaaatgaa
     6061 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
     6121 attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
     6181 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat
     6241 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
     6301 gaacgaaatg gcattcagat cgaaatggaa cctggggatg ccgcattgct gaaagaacat
     6361 actgttgatt ttatctcctt ttcttattat tcttctcgag tttcgactac tgacaacgaa
     6421 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctggaagca
     6481 agtgaatggg gttggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
     6541 gatcgttatc aaaaaccact atttattgta gaaaacggct taggtgcagt cgatacgccc
     6601 gatgagaatg gttatgtgga agatgattat cgaatcgact atctggcagc acatatccaa
     6661 gcaatgaaag atgcagtaga attagatgga gtcgatctat taggctatac gacttgggga
     6721 tgtattgatt tagtatcagc tggtacaggt gagatgaaga aaaggtacgg attcatttat
     6781 gtcgatcgtg acaacgaggg aaatggcaca ttagcccgtt cgaagaagaa atcattcaac
     6841 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaatgattg atcaatgatt
     6901 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
     6961 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
     7021 aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact
     7081 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggcgcccct
     7141 ggcggccaaa tgaacaatac ggcagaaatc gaaaactatc ctggtttcga ttcaatcatg
     7201 ggtcctgatc tagcttataa gatgtatgaa ggtgtttcaa gatttgggac agaaaatgct
     7261 tatggaatcg tgcaagatat caaggatcac ggcagccaca aagaagttat ttgtgaagat
     7321 aaaacttacg aaggaaaaac agtcattatc gcaacaggct gtatccaccg taaattaggc
     7381 gtacctggcg aggaagaatt tgctggacgt ggcgtttctt actgtgcagt ttgtgatggt
     7441 gcgtttttcc gtaacaaacg tctagtagtg atcggaggag gagactcagc agtagaagaa
     7501 gccatctatt tgacacaatt tgcttctgaa gtagtcatcg tccaccgtag agatgaatta
     7561 cgtgcacaaa agatcatcca agaccgtgca tttgcgaatg aaaaaatttc ctttatgtgg
     7621 gatacagtcg ttgacgaaat cgttgggaat gaaatggtag tgacaggtgt gaaagcacgt
     7681 aatgtcaaaa ccggcaatga gtttgatttg gcagcagacg gagtattcat ctatgttgga
     7741 ctggatccat taactgcacc atttagagaa gcagggttga cgaacgaagc tggctggatt
     7801 ccaacagaca acgaaatgaa gacgaatata ccaggcgttt ttgccatcgg agatgttcgt
     7861 gaaaaagatt tgcgacaaat cactactgct gttggtgatg gtgggatcgc tggacaacaa
     7921 gtgtataaat acttggaatc tttagaagaa acaactgcat aagtttaaaa agagtggaag
     7981 aacggtttca attgttcttt tactcttttt tttcgcgtga aatctactga atccgattta
     8041 tgcgaatact tgcaaccgtg cttgataaaa gaatcctttc ttgatttaag cgttttttgg
     8101 tcctcatact tttatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa
     8161 agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat
     8221 ttagaagaaa acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa
     8281 gatgcttttt atgcaccgtt ggaattcggt actgcaggca tgcggggaat cttagggcct
     8341 gggatcaatc gaatgaacat ctatacggta agacaagcaa cagaaggact tgcacgtttc
     8401 atggatacac aaggcgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc
     8461 catcaatcac cagaatttgc catggaagca gccaaaacat tagcacatca caatatccca
     8521 tcttatgtat tcgaaagtct acgtccaaca ccagagcttt cttttgcagt ccgtcatttg
     8581 aagacgtttg cgggaatcat ggtaactgca tcccacaatc cagcagctta caacggatat
     8641 aaagtctacg gcgaagatgg gggacaaatg cctcctgcag acgcagatgc attgacgaaa
     8701 tatgtccgtg aagtcagcaa tccgttaaaa gttgaagtat tatctgacga agaagctgaa
     8761 catagcggat tgatcacgat catcggagaa gaagtggatg ccgcttatct agaagaaatc
     8821 aaagcagtaa cgattgatca tgaattggtg gaaacaatgg ggaaagattt gaaacttgtg
     8881 tacacaccac ttcacggaac aggaaaaatg ttgggcgaac gtgcgttgaa acaagctgga
     8941 ttcgaacaat tcgtactagt tccagaacaa gctgtcgcag acccagattt cactacagtc
     9001 aaatcaccaa atccagaaga acactctgct tttgagtatg cgatacgact tggcgagaaa
     9061 gaaggcgctg atttgctgat tgcaacagat cctgatgcag accgtttagg tgcagccgtt
     9121 cgtttgccaa atggctccta tgaagtattg accggaaacc aaatcggagc actgtttgct
     9181 caatatattt tggaagcaca ccaatcgcga ggcactttac ctgaaaacgc agcggtattg
     9241 aaatcgatcg tttctagcga attacctaca gcaattgcgg aacactatgg ggctaccatg
     9301 ttcaatgtat tgacaggatt caaattcatt gctgaaaaaa ttcaacaatt tgaagaagat
     9361 cattcccata cttttatgtt cggattcgaa gaaagttacg gttatttagt taaacctttc
     9421 gttcgcgaca aagacgcgat ccaagcattg ctcttattag cagaagtagc agcttactat
     9481 aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac ctatggctac
     9541 tacgcagaaa aaacgatttc tgtgacaatg agcggacaag aaggttctgc gaaaatctct
     9601 gctttgatgg aaaaattccg tgaagaagca ccaacagaat ttgccggagc aaaagtcatc
     9661 caaacagaag acttcaaaga gttgacgaga acagaccgtg aaggaaacgt agaaaaacta
     9721 acgacaccgc catctgatgt attgaagtat acgctggaag aaaatggctg gatcgctatc
     9781 cgtccttcag gaactgagcc gaaaatcaaa ttttacatcg gtgtaaaagg aaactcttca
     9841 gaagacgcag ctgaaaaagt agccgcttat gaagcgatta tccaaaagat gacagaagaa
     9901 taaaaaaact tgagactgat ggaaaaagtg gtaaacttta gaagtcagtc ttttttcaag
     9961 ttggaggtga atggattgcc agacacgcaa aaaacaaaag tagagaaagt cagccaacta
    10021 ttcaaaatgt tgagtgaccc cactcgtctc aaaattctgc tgtatctgaa agacggggaa
    10081 caaaatgtga cagcaatcac ccaagcagtg gaaatggaac aatctgctgt atcgcatcaa
    10141 ctgcgtttat taagagaaaa ccatgtcgtc aagtcccatc gtgagggaaa agcgattttg
    10201 tatagtcttg atgattctca cgtactggat attttgaacc agacactcaa acatgtggaa
    10261 catcaataac gataggtgca aatagagaca aactggactg tgtcaacgaa tcttcttcgc
    10321 tgacacagtc cagttttttt taagaaaaag acaagaaaaa ctgttagtct gtttcttcgg
    10381 tggtttcagt tccataataa ctatgatctt ctaggtctaa agcattcaaa ataagcgaag
    10441 ccgtttcttc aatagataaa gatgccacat tgatcactac acatcctagt ttttcataaa
    10501 gatcattcgc aaaatcgagt tcagctctaa ttttttcgat atcggaataa gcagtatcag
    10561 ggtttaatcc gtaagagcgc atccgttctt tacggatatt atttaaaata tctgggttat
    10621 tcgttagtcc cacgattttt ttaggatcga tttcccacag ctgtttggga atatgtgctt
    10681 ggggaatcaa aggtaaatta gcaactttta aatttttatt tgctaaaaac aagctcaaag
    10741 gcgttttgga agttcgggat acgcctagca aaacgacatc tgcttctaaa aaaccacgag
    10801 gatctttccc atcatcgtat tttacagcaa attccattgc tttgatccgt ttgaaatagt
    10861 tctcattcaa taaatggagg gcaccaggtt gccgaattgg cttgatacca gttaaacgtt
    10921 caatttctga tatcggtggc gtcatcacat cgaaactaaa taattgatgt tcttcacaaa
    10981 aagatcgtgt tatctcgatc agttcttgat tgattaatgt atgcaggaca agtgcttttt
    11041 cttctaatgc atcttgcaat gcctgcctta atgttttttc atctgtcacg aacgttctgc
    11101 ggaataggtt gaactcaacg gtaggatact gagccatagt agcttgcgct agttttgtcg
    11161 cagtttctcc agcagagtcg gagatgacaa aaaaagtaac gatttctgta gaatcggaag
    11221 acatggataa aacctcactt gcattttttt tatgtttatt ataccatact ctagaatgcg
    11281 tacttcgtaa tcattttgat ttggaggaat aagtcatgaa aaaacagaca ttgatcgaac
    11341 aatcattaga aacattgaaa aataacggtt ttaaatatac taaaaaaaga gaagcattgt
    11401 taacttattt agtgaagcgt aaccgctacg tatctgctaa agaagttttt gactatatga
    11461 atcaggaatt caaaggggta agttatgata cgatttatcg gaatctccat gattttgcag
    11521 agatcgattt actggaagaa accgatttaa atggagaaat gaagtttcgt tttcattgct
    11581 gtcaggggga aatcggccac caccatcatt ttatctgtac agtgtgcgga acaacgaaag
    11641 aaatccatat gtgtccgatg aattatttcg aagaacagct aggtggatgc acgatcgaag
    11701 ggcatagatt cgagattttc ggaagatgtg aaaattgtca ataaggattt aaataaaata
    11761 acatttgata atttaaaaaa aaagcaagat ttccttgacg gtcaaaacag cattagatat
    11821 aatatttaca ggacagtctt gttttttgag atatatttca ataacaatta cggttacttt
    11881 ttaagctcgg agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact
    11941 tgacgatgct cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc
    12001 tcgtaaacgt gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc
    12061 tagaaaacgc aaaaaattct agttctgtga tgaattggag ttggtagtgt gtcactacta
    12121 agtagattaa acgatgacat gaaagcagcg atgaaagcga aagataagga atctttacaa
    12181 gtcatccgaa tgatcaagtc atctatacaa aacgagcaga ttaaagaagg tcacgacttg
    12241 acagaagaag aagaactaac ggtgttgtct cgcgaaatga aacagcgacg tgattctttg
    12301 catgagtttg aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag tgaaattgta
    12361 atagtcgaaa aatatatgcc ggaacaactt tccgacgaag aaattcgaca acttgttcaa
    12421 gaagcaatca ctcaaactgg tgcttcatca atgaaagaat ttggcaaagt tatgggtgca
    12481 attatgccta aagtcaaagg aaaagcagat ggaaaccaag tgaatgcgat tgtgaaagaa
    12541 ctattacaat cttaatttta tcatttaaac tgcggacagt gattgtccgc
//
LOCUS       DACEDO010000080        12289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEDO010000080 DACEDO010000000
VERSION     DACEDO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.080"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..154
                     /locus_tag="IWO52_002381"
     CDS             <1..154
                     /locus_tag="IWO52_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0356627.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /locus_tag="IWO52_002382"
                     /pseudo
     CDS             complement(235..1218)
                     /locus_tag="IWO52_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="IWO52_002383"
     CDS             complement(1613..2455)
                     /locus_tag="IWO52_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0356628.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="IWO52_002384"
     CDS             2780..3196
                     /locus_tag="IWO52_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0356629.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="IWO52_002385"
     CDS             3193..3663
                     /locus_tag="IWO52_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0356630.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="IWO52_002386"
     CDS             3677..4450
                     /locus_tag="IWO52_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0356631.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="IWO52_002387"
     CDS             4464..5276
                     /locus_tag="IWO52_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0356632.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="IWO52_002388"
     CDS             5330..6295
                     /locus_tag="IWO52_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0356633.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="IWO52_002389"
     CDS             6432..9155
                     /locus_tag="IWO52_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0356634.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="IWO52_002390"
     CDS             9168..9800
                     /locus_tag="IWO52_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0356635.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10005..10232
                     /locus_tag="IWO52_002391"
     CDS             10005..10232
                     /locus_tag="IWO52_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAQ0356636.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10301..10567
                     /locus_tag="IWO52_002392"
     CDS             10301..10567
                     /locus_tag="IWO52_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0356637.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="IWO52_002393"
     CDS             10557..10826
                     /locus_tag="IWO52_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0356638.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11005..>12289)
                     /locus_tag="IWO52_002394"
     CDS             complement(11005..>12289)
                     /locus_tag="IWO52_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0356639.1"
                     /translation="IKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEIRRCPLC
                     KQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTYIVDRNC
                     FIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWLPRCLLM
                     DEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKVKVIVTD
                     MNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQAKYYRQL
                     KALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFYYQEILQ
                     AFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKIENLHTH
                     IKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4019 a         1991 c         2408 g         3871 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc ttttttatgg ctctttgtca aataggactg atagagtgag ataagaacat
    10981 ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa tcagattatt
    11041 tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag cgactcgttt
    11101 caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag gcaattcgag
    11161 ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat gcagttcttt
    11221 tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct ctttgtcgtg
    11281 aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt cagagatcga
    11341 aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat tgcgccattt
    11401 ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag acaacgcctt
    11461 taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt tcatgattcg
    11521 tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac gatccacaac
    11581 gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat tcatgtccgt
    11641 cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat aagaaacgag
    11701 agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc catccataca
    11761 agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca ttaataaaca
    11821 acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga gtgtccgaga
    11881 aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt gtttttcagt
    11941 tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc tatcgacaat
    12001 gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt gtttcgctaa
    12061 agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt ttttctttcc
    12121 gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc gacgaatttc
    12181 tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct tcgattccac
    12241 atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgata
//
LOCUS       DACEDO010000081        12171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEDO010000081 DACEDO010000000
VERSION     DACEDO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.081"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            592..1920
                     /locus_tag="IWO52_002395"
     CDS             592..1920
                     /locus_tag="IWO52_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0356640.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="IWO52_002396"
     CDS             2122..2955
                     /locus_tag="IWO52_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0356641.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="IWO52_002397"
     CDS             2971..3708
                     /locus_tag="IWO52_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0356642.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="IWO52_002398"
     CDS             complement(3863..4831)
                     /locus_tag="IWO52_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0356643.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="IWO52_002399"
     CDS             5063..5896
                     /locus_tag="IWO52_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0356644.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="IWO52_002400"
     CDS             5930..6769
                     /locus_tag="IWO52_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0356645.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="IWO52_002401"
     CDS             6795..7355
                     /locus_tag="IWO52_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0356646.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="IWO52_002402"
     CDS             7554..9275
                     /locus_tag="IWO52_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0356647.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="IWO52_002403"
     CDS             9440..10582
                     /locus_tag="IWO52_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0356648.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="IWO52_002404"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="IWO52_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0356649.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2527 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DACEDO010000082        11757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEDO010000082 DACEDO010000000
VERSION     DACEDO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.082"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(258..2333)
                     /locus_tag="IWO52_002405"
     CDS             complement(258..2333)
                     /locus_tag="IWO52_002405"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0356650.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2335..3252)
                     /gene="glyQ"
                     /locus_tag="IWO52_002406"
     CDS             complement(2335..3252)
                     /gene="glyQ"
                     /locus_tag="IWO52_002406"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0356651.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3636..4448)
                     /gene="recO"
                     /locus_tag="IWO52_002407"
     CDS             complement(3636..4448)
                     /gene="recO"
                     /locus_tag="IWO52_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0356652.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4591..5490)
                     /locus_tag="IWO52_002408"
     CDS             complement(4591..5490)
                     /locus_tag="IWO52_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0356653.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5505..5906)
                     /locus_tag="IWO52_002409"
     CDS             complement(5505..5906)
                     /locus_tag="IWO52_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0356654.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5884..6360)
                     /gene="ybeY"
                     /locus_tag="IWO52_002410"
     CDS             complement(5884..6360)
                     /gene="ybeY"
                     /locus_tag="IWO52_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0356655.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6377..8569)
                     /locus_tag="IWO52_002411"
     CDS             complement(6377..8569)
                     /locus_tag="IWO52_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0356656.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8593..9564)
                     /locus_tag="IWO52_002412"
     CDS             complement(8593..9564)
                     /locus_tag="IWO52_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0356657.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10269..11648
                     /gene="brnQ"
                     /locus_tag="IWO52_002413"
     CDS             10269..11648
                     /gene="brnQ"
                     /locus_tag="IWO52_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0356658.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3196 a         2451 c         2138 g         3972 t
ORIGIN      
        1 aattcttgac gcaaccactg ttttcttgac tagggcaatt tacgattaaa aagtaaataa
       61 aacattaata agataacaat taatcgattt tatttttatt tccttaattt tcataggtat
      121 tttcaaaagt tacacaaaaa ataaaataac aaaaaaaatt aacattgaat taataatagt
      181 tatttatatt tataacaaat aaaccagaga aacagcctat tttgtgcctg ttctctggtt
      241 tatttacata ttagaaatta ttttgtgatg atttgatcta aacttgctag tgacaaagcc
      301 atggcagcaa tttgacttaa ttgcgttaat cggttcttac gtactgctgg ttcttctgcc
      361 atgaccatcg tctgatcaaa ataattttcg atcagtgggc gaaggcttac aagctgatca
      421 taattttcgt tgatcgtttg gtcagcaaag cttgttttca ttgcctctac tgctttgtgc
      481 agttctttct ccgcttcgtt ttcgaacagt tcagggtcta cttctgattg tttttcaagc
      541 tccgcttttt tcgctaagtt cacgacacgt gtcaaagctt ccatcgatgg tttgaagtct
      601 tcatctgcaa gatgctgctt gaacaattgt gctgctgcaa atacttttgt cagatctttt
      661 tgttctgcgt tcaaaacagc ttcgataaca tcatgacgga tattcttcgt tgtcaataat
      721 tggcgcagac ggcctttgat aaagtcgatc acctgttgtt gtcccgctga caagccaatg
      781 ccaaaacgat ccgtatcttt actgataact tcactaatcg tttcttgcag aacagacaaa
      841 ggaaaggccc attttttcga ttcgatgatt cgaacgacac catatgcttg acggcgcaaa
      901 gcatatggat cgtttgagcc acttgggatc atcccaactg caaagaaaga aaagatacta
      961 tctaatttat cagcaatcgc taaaacagaa cctactgtag tctcaggtag atctccttca
     1021 ctggatatag gcatgtaatg ttcacggatt gctgttgcta cggctggttt ctcgccttta
     1081 agcagagcat atttttctcc cataatccct tgtagttcag ggaattctcc taccatgttg
     1141 gttactagat caaatttata gatttcagca gcgcgttgca gatccatcaa ttcttcctca
     1201 gatagtccta cttgttcgcc tatgatgcga gcgatgactt gtacccgttg cattttctca
     1261 tagatgctac cgattttttc atggaaagta acagttttta atttttcaac gtattggtcg
     1321 attgaagctt tcttgtcttc attataaaag aattccgcat cttctaaacg agcagtcaat
     1381 actttttgat tgcctcgtac cacattttcc aattttaccc gatcgccatt acgagcagaa
     1441 ataaaatgag gaagcaacat accttgatcg ttccgtactt cgaaataacg ttgatgttct
     1501 ttcatagaag tgactagtac ttcttcagga acagaaagat atttttcctc gaagtctcca
     1561 gaaaagacag ttggatattc gactagattg ttgacttctt ctagtaaatc ttcatctaga
     1621 tcaatgatcc agtgattttt ttctgctaat tcgtttgctt gtgtaacgat catttcttgt
     1681 cttttttgcg gtgaagcaat aacaaattgt tcatttaatt tttcttcgta ttcttttgca
     1741 tgcgcaaatg ttacatcttc gcctaagaaa cgatgacctc ttgaagctct gtctgttttg
     1801 acatccaaga tctcaaacgg gattatttcg tcatctaaca atgccacgat ccagtgaata
     1861 gggcggatat attcaaaatc gtattctgcc caatgcatcg ttactgggaa agtgaggctt
     1921 ttaatgacat ctgctaaacc tgtcaatact tcttttgctt ctttgccgtg agtgtatttc
     1981 gttacataga catattccac gccttttaat tctctaaaag tgatagcatc tgtcgtcaga
     2041 ccttgtccgc ggacaaatcc ttcagctgct ttcgtccagt ttccatctgc gtccaaagca
     2101 atttttttgg ctggtccttt gacttcttct tcgctgtccg cttgtcgttc tggaatattt
     2161 gttactttga ttgctaaacg gcgcggtgtg gaaaatgttt cgatcgtatc ataagtcaat
     2221 tggttttcat ctaaaaattt tgctgttttt tcttctaatt gtttcatgct tggtgtgact
     2281 acatgagcag gcatttcttc cagcccgatt tcaagtaata agtcttttgc catcttattc
     2341 agcctccttc acgttttctg gatttgctgt gtttttattc aataaaggga atcctaattt
     2401 ttcgcgttct gccacaaaga ttttggctac agcacgagcc atgttgcgga tacgggaaag
     2461 ataacctgct cgttctgtta cagatactgc accgcgagca tctaataaat tgaatgtatg
     2521 gctgcatttc aaaatataat catacgctgg atgaacaaga ttttcatcga tacagcgttt
     2581 agcttctttt tcaaaacggt caaaattgct tagtagtaag tcttggtcgc ttacttcaaa
     2641 agaatatttg gaatgttcat attctggttg tttgaagatc tcgccatatt tcacgccatc
     2701 agcccattct aaatcataga cactttcaac ttcttggata taagaagcaa gacgttctaa
     2761 tccataagtg atctcagaag taactggttt acaaggtagt cctccgactt gctggaagta
     2821 cgtgaattgt gtgatctcca tcccatcaag ccatacttcc cagcccacac cggcacaacc
     2881 catcgatggg ttctcccagt tgtcttcaac aaaacgaata tcgtgttcta atggatcaat
     2941 tcctaaaagg cgcaagcttt ctaaataaag ctcttggata ttttcaggtg aaggtttcat
     3001 tacgacttga aattgatgat gctgatacaa acggtttggg ttttctccat aacgcccgtc
     3061 agctggtcgg cgggatggct ctacataagc agcattccat ggttccggtc cgattgcccg
     3121 taagaatgta tatgggctca tcgtacctgc tcctttttct gtatcataag cttgcatcag
     3181 catgcagccg tttgatgacc aaaatttttg taatgttaaa atcatatctt ggacggtcaa
     3241 ttttttactc atctgttttg cccctttcaa tttttaagcg ttgctgtcaa taagaataca
     3301 ggaaagaaaa cgacaaaaag tccctatgtc tgattacaga catagggacg aataatttcg
     3361 cggttccacc ctacttccga ataaagattc ggcagcttca tttatcgtac tcgcaagtgc
     3421 ctcttcaaaa atcgtttggg tccggcttcc actctcccga actcgctaaa ccacgtgttt
     3481 tttactgatc ttggtcaatg tacgtattca ttttgcagtt ttcttgtttg tataatagat
     3541 cctctcagtg aacgatctat ttaatctacg gttaataata gacggaagtc cctgtatttg
     3601 tcaaatagtt ttgatttttt tgttgtatct ttcatttatt cttctttttc aggtatctct
     3661 ttttcaggca tatttttctc tggcgtcttt aatacgtgtg cccaagactt catttgatca
     3721 atgaactttt tgctttttag atgaatacct acatattcat catacaactg gtcaatgagg
     3781 tcacggattg cctgcttcgt ttcttctttc aagtggattc cactgatctt atcataagaa
     3841 atagccgaaa acattcgaac gaaataaaca gcgcgaggat ctgcatgata acggtgttcg
     3901 tccattggcc agtgtttctc gcatatcaca ccgccatatt tggaagaata gtcaaatttt
     3961 ccttttgttt ccccgcatac gacacagtgc gtccaaattg gcgcgattcc gaagcgttgc
     4021 agtatttgaa cttcaaagat attcgtgatg atttctgcat catctccttg gtccatccgc
     4081 tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat attgatgatc ttcgattgct
     4141 gcatctacta gatttagcaa atacgtacca taaccgctca aaaagatatc ttcttggatg
     4201 ttccgaaacg catggacgtc tttgacactg cttaaaaacg acaaaccttc acttcgaaaa
     4261 ttaccgatat agactgcttc ggtaaagggg agcaaagccg gagctaatgg ctgattttgc
     4321 cgatggattc ctttgatata aaacatcatt ttgccagaag attccgtaaa gattttgact
     4381 aatttatctt tttctttgta attacggcta gataaaatca agcctttcga ttctgcaagc
     4441 tggctcatat tctccctcct aaaaaataaa tctggaaaag cagattctgc cgatccagat
     4501 tatttatgtt acgatcttcc gctgtttttc tcaacgtacg gatttttatc cagtttattt
     4561 ctatacctaa cctataagaa gtccagaata tcagtactcg tcttttcggt agccgaagtc
     4621 ttgcagatag acttttttgt ctcgccagtc tttttggact ttgacccaaa gttctagaaa
     4681 tactttgtct cctaaaagat tttcgatatc ctttcttgct ttcgttccta cttgcttcag
     4741 catttttccg cctttgccga taatgattcc tttttggcta tctctttcga cgatgatcgt
     4801 ggcctggata tggattttat cattctcgtt tcgcttcatc gaatcaacaa cgactgcgac
     4861 agaatgaggc acttcgtctc gtgttaaaaa caagactttc tcacggatca attcagaaac
     4921 gatgaagtat tcaggatgat cagtcacttg atcatctgga aaatattgtg ggccctctgg
     4981 catttcactg actaatacat ccattaagcg ttcgacattg tttccttctg tagcagagat
     5041 cggaatcact tgtgcaaaag gcatctgcgt cgaataatct tcgatgattc ccagcaactc
     5101 gtctggatgg atcttatcga ttttattgat aatcagataa actggagaat gcacgttttt
     5161 tagacgttca ataataaaat cgtctccccg gccgcgtttt tgatcagcac tgatcataaa
     5221 taagactgca tccacttctt tcaacgcact gtaagctgct tctaccataa aatctcctag
     5281 acgatgcttt ggtttatgga ttcctggtgt atcgataaaa atcagctgag cttctggtgt
     5341 agtatatact ccttggattt tatttctagt tgtctgtgcc ttgtcactca taatggcaat
     5401 tttttgaccc acgattcggt tcaataatgt ggattttcct acattcgggc ggccgacgat
     5461 tgcgacaaac cctgatttat gttctggcat atacgttctc ctttttaatt aaacaacaaa
     5521 tgatatattt tagggacaaa taaaatcaac ccaatgatga cagcaaaaca agcggtcact
     5581 aaaactgctc cagctgccat atctttgatt tttttcccaa tcggatgaaa atgaaaatcc
     5641 gtaaacatgt ccactacatt ttcaaaaaca gtattgatga tctctactat ccaaacgaga
     5701 aaaacagcta aaagcagcca taaccactct atccgatcaa gctggaaaat cacgcccata
     5761 aataaagcaa ggataccgaa cgcaacatgt ttcctcatat tgcgttcttc ctcaaaaacg
     5821 gttttgactc cttggacagc aaattccacg gacatcagga aatgtttgtt tttctctacg
     5881 tttttatctt ttgagtccat aggcatccaa gatctctttc tgtaaaccaa acatttcttt
     5941 ctcatcttct ggtgtcatat ggtcataccc gttgatatgc aagaatccat gtaccgctaa
     6001 aaagccaagt tcccgatcat atgaatggcc gtaagcttct gcttgttcag cagcacgttc
     6061 aatagagatc atgatgtctc ctagtacacg aggcaatgga ttctctccat cttcaaaaat
     6121 cactgggatt tcgtcttcgc cttcttcttc cagagcaaaa ctgatgacgt ctgtcggtgc
     6181 atcttttcca cggtaatcac gattgattac ttggattgcc gcattgtcca tgaaagtaac
     6241 agacatttct gtttcctctg gcagctttaa ataagatgct gcaaattgca gcaagtcatc
     6301 gatttctttc attttctctt ctgagacttg gtttgtttca tccataaagg taatatccat
     6361 tatacatcgc ttctttctat tcttcttgtt cttcctttat cttctcaact tctgttttca
     6421 ttttaggata tttgatccga gaatggaagg tgctacttaa accgctgatc aatgattttt
     6481 ccacttttcg gatttctttc aaggtcagtc cactatcgtc taattgacca tcggaaatac
     6541 gctcttcgat aagattatgg acaaaacttt cgattttttc gctcgtcgga tgatccattg
     6601 cccgtactgc agcctcacaa ctgtcagcaa tattgacgat ccctgcttca cgactttgcg
     6661 gttttggtcc aggataacgg aattgctctt ctgttacttc cggattgcgt tctttcgctt
     6721 tgatgtagaa ataacgcatt agcgtggttc catgatgctg tttgcaaata tcgatcacca
     6781 tttgcggcat attataatct tccaatattt tggctccatc tgtcacatgt ccaaagataa
     6841 tatgtttact gtcttctggc aataagaaat tgtgcgggtt ttccgctcct gaaggcaagt
     6901 tctcaacgaa aaagctagca tgtttgatct tgccgatatc atgataatag caagctactc
     6961 gagttagcaa agaacgtccg ccgatttctg ctacagcgtt agcacttaga ttagctacca
     7021 tcatactatg atgataagta cctggtgctt cttccagcaa ctgtttcaat aaaggatgat
     7081 ttggattgct gagttcattt aatacaatta cactgtcatc attgaccatc aattcaatat
     7141 atgggtgtag accgatcgtc agcaagaatg agaagagtgc accagtgaac ccgcagacga
     7201 gcattaacca agtcgttgca tcaccaaagc tcattccttg atagacgatc aaaatgacat
     7261 ccatcatcac aggaaaaata atgacccaca tggcagcaga taatccttgt tctgagatcc
     7321 gtttgcgtct gacaatcgtt gccatgagcc cagagaaaac ataagacatc aagataatcg
     7381 ttagaaagtt cgtgccaatc gaatcataaa aaatgaaaag tgcggatact gcttggaaaa
     7441 taccagccat gatccctgct cttcgattca agaaaaagct taaaacaagt ggtgcaaaag
     7501 cagcgggata aaacagcggg atataagtag cttgttccgt ttggaataat tggaaaaatt
     7561 tcatcagcac tatgctgact gacatagctg tcacatagaa aagcacgtat tttgttcttt
     7621 ctcctgcatt ttggaattga agtgaattat aaatcagtac gccgatttga agcaaaacag
     7681 ctaaaaccat ggcaattagc ggaaaaatcg aagttgtttg attagtcagt cctagtaatt
     7741 ccaatttttt catagctgaa gcatcgattt gattaccttc acgaacaatg atttctcctt
     7801 ggaagatcat cactggttgt acagcttctc gtgcggattg tttcaattct tctgtctttt
     7861 tctcattcaa tacgtcattc actacgatac cttgatccac taagtagcga atcatttgtt
     7921 gttgtgctgg tgtgacattc aaatattgga tttgctcttc tgcttcttgt ttaaaagctt
     7981 ccaattcact ttcacgaact tgtttgctca tctgttcgtc aatgagcttc agactctcat
     8041 ccctcacagt atggatctca gtttcagtca tttcaaatat cgtcgaataa aaattgttcg
     8101 gtagtttttg ataaaacgca acattttctg cattcacatt ttcaaaattt ttcttcagcg
     8161 cggcgatccg ctcttctgta gtaggctttg ggatattttc atcttccttt gccttatcaa
     8221 tcttttcgtc ataagatttg ttgatggcat cattcgtctt atcgatcaat tcaaatagtt
     8281 gcttgatccg attgttctgg tcatctgcaa gatctttttg gtaagtgtat tctggagtga
     8341 ctgcttctgc tgccagtttt cttttttgtt cggtttcttc cgtgttctcg atcgttttgt
     8401 tcgcacggat actttcttct gctacttggc cttctctgaa atcattggcc ttttgtttga
     8461 cactactaaa tgtaacgccg cagataatga taaaaaacaa gaacagcagg aaaggcaatg
     8521 ctgcctttcc aagtttttct tgtagtttct gcagtgtttt attcaacatg tgcttatcct
     8581 cctaagcaaa gcttattctt gatggagatc tgccttttca taagctttga tgatttcggc
     8641 aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga ttttcttgat
     8701 ttgctttaat gttcgttctg catgaacaag accacttgtt gtccctcgag gcagatcaat
     8761 ctgactcgta tctccattga caatcatttt tgattgataa ccaagacgtg tcaggaacat
     8821 tttcatctga gcaactgtcg tgttctgtgc ttcatccaaa ataacgaatg catcatccaa
     8881 tgtccgcccc cgcatgtaag ctaaaggagc aatttcgatc acgcctcgtt ccatcaatcg
     8941 gttcgtatga tctaatccaa aaatttggta taaagcatca taaacaggac gcagataagg
     9001 atctactttt tcttttagat ccccgggaag aaagcctaga ttttcccctg cttccacagc
     9061 aggccgcgtc aaaatgattt tttgcacttc accttttttc aaggcagcga ttgctaatac
     9121 tactgcaaga aaagtttttc ctgtcccggc tggtcctacg ccaaatacga tatcatgttt
     9181 ggctaccgat tcaacatatt tcttttggcc gctgtttttt acccgtatag gttttccatt
     9241 acggtctttg acgatctctt cctcatacat ctcaacaaaa taatctaatg tgcctttttg
     9301 ccccattttc agtgccgtca cgacatctgg tgtacttaca tggattcctc gtttgatcaa
     9361 ttcctgcaat gtccgaatga ctgaggcagc caaggaaatc tgggattgtt caccaatgat
     9421 ctgaatgatt tctccacgtg tatggatggt tgtttctgtt gtttcttcaa tcagttttat
     9481 atgcttgtcg tgtgcaccta aaagcatgct gacatcatca gcagccgtca atctaatctc
     9541 taaagaacta gactcttccg tcaaaaatag acatcccctt tttttcgatt cattctttcc
     9601 tattttacca tactcagaag aaactatata gcaaataaga aaaggttcta aaagagttgc
     9661 ttaaccataa gtaccaagaa acgttaaaaa atagctgctt ccattctcta aaggtctcgt
     9721 ttgaataaaa aaagctgatc atgtaagcta tgcataagat agaggacaag tattccgtct
     9781 aacttagcaa gtattctctg ggtatttttc ttgtttcaaa aaaagaaaac aaaatatttg
     9841 aaatattctt gcatttcaaa aaaaacaggt gtattataac gtcaacaaat taatatacag
     9901 caaagcgtcg aggaaaacag taggctattc agcgatttat tcccagtgag tcatgtaagt
     9961 gagaaatgac aataaatcga atagacgaac atcatttccg agtcaatgtc gaaaacgtag
    10021 atcattgtgg ctgcctccat tatcaggcta gcggataact ttatcgaaaa gcgtccgtga
    10081 atgaagaagc ttttttaagc gaaaaaaggt ggtaccgttc tagaatctag agcccttttc
    10141 ccaagtggaa aagggctttt tttatatttt aaggtggtgg ttttcatgga atctgatatt
    10201 agtggtattt ctagtttctg caacgtttat tttgttactc aaaatgataa ttattggagg
    10261 aaaaatttat ggaagaaaca aaaacttatt catggaagca gttattgatg gtcagttcac
    10321 ttatcttcgg gatgttcttc gggtcaggta atctgatctt tcctgtccac ttaggtcaaa
    10381 tggctggatc aaactggatc agcgctggtg taggatttgc catctcaggc gctcttttcc
    10441 cattattagc tatcttagct gtagtagtga caaaaagtga tggtctgtat gacttagcta
    10501 aacctgtcgg aaaatggtat gctgccttgt tcttgatcct tgttcattta acgatcggac
    10561 cattctttgg tacaccgcgg acagcagcaa ctgcttttga aatggctgct aaaccctttt
    10621 tgcctgaaaa atatgattca ttaggtatgt ttttattctc tgctcttttc tttttacttg
    10681 cttatttcct tactgtacat ccaacacgtt tggtcaaata tgtcggaaag tatttaaact
    10741 ccttgtttct tgcattatta gctatcattt ttgtcgtttc tttattccaa cccatgggga
    10801 atctaaacca atctgctgca gattcttatc agacgaacgc tttgacaaat ggcgttttag
    10861 aaggatacaa cacggttgat gcagtagctt tacttgcttt aagtgtcact tttgtccatg
    10921 cagttcgtga tcttggtttt aaagataaaa aagtcacaga attaacagca aaagctggta
    10981 gcctgagcgt tgcgttagaa gtcatgattt attttggtct ggtccttctc ggtgcgatga
    11041 gtttgaataa gatgtcttta tcagaaaatg gcggaactgc actttctcag atcactaatt
    11101 tttatttagg taacttcggt gctagcttct taggggtaat ggtcacatta ggtgtcttca
    11161 ctactgctat gggactcgtt gtttcttttg cacaagattt ccataaactg ttccctaaag
    11221 tcagttatat gacatggctg cggttaacga cttttgtctc ttttgtcgtt gcgaatgccg
    11281 gattggataa tatcatccaa tggtctttac cagtattgat gcttttgtat ccattgtcac
    11341 ttgcgttaat cttactttcc ttaacagcaa aattcttcca aaagagtcca tttgtctatc
    11401 aagtaacgat gttcttttct gccgttccag ccgtattgga tatgttggca aattcgcctg
    11461 cattagtgag tcagcaaact gtcgtagcaa gcttactagg attttatcat cattatgttc
    11521 cttttgctgc attaggtctt ggctgggtcg tcccaacgtt gcttggttac gctggcagct
    11581 tgctctttta ttatgtatat cgcgtatcag gatataaaca agaagcaagt gagttaccag
    11641 aagaataacc aaatcaaaca caatttaaca taagagatat taaacaggaa aaaaaacagc
    11701 tagaagaaag aataagattc tttcttctag ctgtttttta gttctttttt tacgaac
//
LOCUS       DACEDO010000083        11736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEDO010000083 DACEDO010000000
VERSION     DACEDO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.083"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(289..2073)
                     /locus_tag="IWO52_002414"
     CDS             complement(289..2073)
                     /locus_tag="IWO52_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356659.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(2073..3824)
                     /locus_tag="IWO52_002415"
     CDS             complement(2073..3824)
                     /locus_tag="IWO52_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0356660.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(3966..4190)
                     /locus_tag="IWO52_002416"
     CDS             complement(3966..4190)
                     /locus_tag="IWO52_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0356661.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4441..6027
                     /locus_tag="IWO52_002417"
     CDS             4441..6027
                     /locus_tag="IWO52_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0356662.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6093..7868)
                     /locus_tag="IWO52_002418"
     CDS             complement(6093..7868)
                     /locus_tag="IWO52_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0356663.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8079..8981)
                     /locus_tag="IWO52_002419"
     CDS             complement(8079..8981)
                     /locus_tag="IWO52_002419"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0356664.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            9260..11605
                     /locus_tag="IWO52_002420"
     CDS             9260..11605
                     /locus_tag="IWO52_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0356665.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         3561 a         2403 c         2187 g         3585 t
ORIGIN      
        1 gaaaaaccca aaaagccgtc catggaacca tgggagactc atcaggttta gcttgcatca
       61 ggcaatcact atccaagttg tatcgttttc tttatattat cgaaagcaaa cgcttttgtc
      121 aacgctttct tagtggttat tagccgcttt tgactagctg tcgtcgtctt ttttaaatcg
      181 ggtgactacg ccactaacgc tcacagatga agaatctgcg aagctctgtt ttattttcac
      241 tctagcgctt agggcaaaat gctactatgc tactgtcaca acctcttttt acacaccact
      301 gctttgcagt ttgtacatat ctgcatacaa accattttgc gctagaagtg tttcatgatt
      361 cccacgctct actatatgac ctttgtctaa aactaaaatc aattctgcat cccgaatcgt
      421 ggacagccga tgggcaatcg ctatcgttgt ccgtccttgt cgcatttttg ccaatccttc
      481 ttggatcaat ccttcggttt cagtatcgat atttgctgtt gcttcatcca atactagtat
      541 tttaggatct gtcacgattg ttcgcgcaaa agtgatcagc tgtcgttgtc cactagaata
      601 gctggcaccg tgttcgatca cttttgagtg atatcctttt ggtaattcat cgatgaactt
      661 gtttgcttga acaaactcag ctgcatggcg aatctgttcg tctgtgatag ctggattcat
      721 catccggata tttcctgcaa tatctccata aaacaagaag gcatcttgca agaccaatcc
      781 cattttctta cgtaactctt ccattggata ttctcgaatg tcatgatcat caatcaagat
      841 ttggccttca taaaattcgt aaaaccgcat caatacgttg atgatcgagc tttttccgct
      901 tccggtgtgc cccactaatg caacggtctc acccggatta gcgacaaaag agatattgtt
      961 caatacattg tgctctccat cataagaaaa gcttacattg cgaaactcga tcttacctga
     1021 agtaatcgct tgatctgctc caggattttg ttgcggtgca tattcttctg tatccatgat
     1081 tttcaaaata cgactaccgg caaccatccc atctgtaaag gtgctcaaaa agtccatcat
     1141 ttgagacatt ggattaaaaa attcctggac ataggtcgta aatgcataga tcagtccaac
     1201 ttctacggga gaatgcaacg catcgatccc aaacaaagtc aacgccaacg caatcgccaa
     1261 tgcatagaga aaattgatga ttggacttaa cagaatcgaa ttcgttttga tcatcgcata
     1321 gcgcgtgtct aaatactcgt tgttcacttg ttcaaattct ttagctaaac gagcttcttg
     1381 gcgaaactgc tggataatac gcatgccgga aatcgattca ttcaatttcg tgttcagctg
     1441 acttaatttt tcccgcatat ttcggtaaat acgagaactg aatttctggt aataccagat
     1501 gacgattcct aaaatcggca agaacaccaa agtccacaag gcaatttctc cattgatttg
     1561 aaacatggca acgaaagaag aaacgactgc aaagatccca gtcaatacca tcaaaaagac
     1621 ataccaaaat tcaaatagcg tttctgtgtc attcgtcact cgagaaacgg tcgatcctgc
     1681 cggtgtctgg tcaaaataac gcatacctag tgtatgcagc ttttcaaata atcttacccg
     1741 aacatactga taggtcttca atgaagccat ggaatacaaa taccattgga aaaaccaaat
     1801 gatacttttg actatgactc caaacaagta cacaccagca aaaaacaaaa taacttgtgt
     1861 cgtagctgac tgtttagcca gatgatcatc cataaaagtt tggatgatcc ttggcaatag
     1921 gacattgatc acagacaggg caaacgcaaa taagatagca acgatgaacg ttcttctaaa
     1981 aggtttggcg aacttcatca gtcgtttgat gatcttcttc tgttctttaa gtggaatcga
     2041 ttttgtccat tccgattgat aattttcatc catcaggcct cgcctccttc tatcttcgct
     2101 tctaattgct gtttttccca cattcgttca taccagccac ctaatgcaag cagctcgtgg
     2161 tgggttcctc gttcaatgat tttcccttga tcgacaacaa tgatttcttt tgcatgcatc
     2221 acgctgctta aacggtgagc tgcaatgatg gttgttttct cttttcttat ttcttttaaa
     2281 ttatgcaaga tagcttcttc tgttttggca tctactgcag aaagtgcatc atctaagatc
     2341 agcaattcag gatttttgat caatgctcga gcaatcgaaa tcctctgttt ctgtccgcca
     2401 gataaagaga ctccacgttc tccaactaag gtatcataac cttttggcaa ttccttgatt
     2461 tcattgttga taaatgctaa agaagccgca tgctctactt cttcttctga aagttttgga
     2521 ttagaaaaac ggatattatc tcgaatggtc atcgaaaaca ggaagtgatc ctgaggcaca
     2581 taaccgattg atcctaataa agcattcaat gaatattctt tgatcgaacg tccgccaaat
     2641 tggattttcc cttgataatc atcgtattca cgcatcagta atttgatgat tgtcgttttt
     2701 cccgcaccag ttttcccaac aatacctaat gtttctcctt cttggatcgc aaatttgatt
     2761 tgttctaatg ctggtgattc ttctttcgga tagttaaaag aggaaatagc tacattcagc
     2821 tcgccttttg ccggggtctg cactgcaccc agttgttcca cgatatgcgt cttttcatgc
     2881 aaaagttcat tgacgcgatc ataagaagca tttcctcttt ccaatacgtt gaacaatcga
     2941 ccaatcgcaa acatcggcca aacaagcata ccgatatagc taataaatga gaccatctgc
     3001 ccgattgaga tcgtgccttc caagacatac tttccaccta aaataattgt tgcaacataa
     3061 gacagaccga taatcaaggt aatgaatgga tcaaataagg agtctaaaaa attgactcgt
     3121 ttgtttttaa taattgcatc ttcaatcttt tcttcaaaat ccgcgagatc ttctttttct
     3181 tgtccaaatg ttttaatgac tttgatccca gtgatacttt cttgtgtctt atcattgata
     3241 ctagaaaaag cttcttgcga atcacgaaaa gcatcgtgga gttttgaccc cagcactcgt
     3301 gaagtaacgg ctagcaaagg cagaggaatc agcgcaatca gtgttaagcg ccaatcaata
     3361 aacaaaatca tcgcaatgat cgttgttccg cccgtaatca ctgaatctgc aaaggtcaag
     3421 attccagctc ctgctacatt ctggattgca ttcaaatcat tcgttgcatg agccatcaaa
     3481 tctccagtcc gatatttttg gtaaaagaga ttgtccattt ctgtaaaatg tttgaatagc
     3541 tgctgccgaa gttctctctc caaccgagct gcacttcccc aaatattcgt ccgccagata
     3601 taacgaaaaa gatattgtaa gaaagctgca aggatcaata ataagatcca ccaggaaatg
     3661 atctttattg ataaattctt atcggcgatc tcatcaatga tgatcccaat cacttttgga
     3721 ggaactaact gtaccaaggc cactgcgaat agggagaaaa tcccaattaa ataatgtttc
     3781 ttttcctgac gaaaaaacca acctaatttt ctaaaaatcg acattttact cctcctttgt
     3841 ctttatcaag agcataggat tgtctgtgct tgtctttttt gctgaatagt ctgaatagtc
     3901 taacaaatta cgaaataaaa aacggactga aaaaatcagc ccgcaaaaag tctttgggtc
     3961 agaacctatt ttttcccttg gttcttcatt tgctgcatca tctggcgaat tttcttctca
     4021 gatggttttt gccccattga catcatcatt gatcgcaaca tgtcctcatt aacgggagga
     4081 tttttttcta aatagtcttt catatattta cgcgctagga aaaagccacc tactgcgccg
     4141 attagcaaag caataatagc aataagtacg acaatacctg ttgataccat ataaatctct
     4201 cctttctatt tccgaattgc tctcaatgat ttattttact aaagtttatt gaaaaaagaa
     4261 aggcgtattc tttagtttca tgcaaaaaat agtggttttt ttagagaaga acaaaatttc
     4321 tttcattaac cagcaagaca caccacgaaa aaaaattagg cataccgaat ctttttcttt
     4381 aattttttct ttctgaggtg tagaatgtaa tcgtagataa ataggaaagg tggttttttc
     4441 ttgaataatc aatccaacaa acaatttcat aagcatcgac ttattgaaca tacaaatggt
     4501 ttatctttag ctgaagtaaa tggaacagtg gatgtaccaa agggaaaaag tttttttaaa
     4561 acacttcttg cttactctgg accaggtgca ttagtagcag ttggttatat ggatccaggt
     4621 aactggtcca cttcgatcac tggtggacaa aatttccaat atttattgat gtcagttatt
     4681 ttgatgtcta gtctaattgc tatgctttta cagtatatgg cagcaaaatt aggaatcgtg
     4741 acccaaatgg atttggccca agcaatccga gcacggacaa gtaagacatt gggtgtcatt
     4801 ttatggatct tgactgaatt agccattatg gcaacagaca tcgcagaagt cattggtgca
     4861 gcaatcgccc tctacttact ttttgatatt ccattggtga ttgccgtctt tattactgta
     4921 ttagatgttt tcgttctctt actactgaca aaagtcgggt ttcgtaagat cgaagcactg
     4981 gttgtctgct taattttcgt cattttattc gtttttgtct atcaagttgg cttgtctaac
     5041 ccagattggc acagtttatt tgctggcttt atccctaaca gtcatacatt ctcagactca
     5101 ccggtcatcg gcggcgaaac tccgctgact ggagctttag ggattattgg tgcaacagtc
     5161 atgccgcata acttatatct tcattcagca gtttcacaaa ctcggaagat cgatcaccaa
     5221 aacgaagaag atgtagcaca agctgtacgt ttttctactt gggattctaa tatccagttg
     5281 tcaatggctt ttctagtcaa tgcactgcta ttgatcatgg gtgtcgctgt cttcaaaacc
     5341 ggttctgtcc aagatccttc cttttttggt ctgtatgaag ctttatctga tccatcagct
     5401 atgagcaatg gtattttgat tgctgtagca aaatctggta tcttgtccac tttatttgct
     5461 gtagcacttc ttgcttctgg ccagaactct actattactg gtacgttgac cggtcaagtc
     5521 atcatggaag gattcatcca tatgcgtatc ccgatttggc taagaagact ggtcacacga
     5581 ttgctttctg tgattcctgt attgatttgt gttttgttta caagtacaaa aggaacgatc
     5641 gaagaacatg ccgctttgaa taacttaatg aatgaatccc aggtgttctt ggcttttgcg
     5701 ttgccatttt cgatgattcc tttactgatg atgaccaaca gccaagcgga gatgggtagc
     5761 cgatttaaaa attcattcat cgtaaaactt cttggctggt tctctgtgat tagtctaact
     5821 tacttgaact tacgaggatt accaggacag atcgaaagtt tcttcggcga gcaagccact
     5881 tccgctcaac tagcattggc tgatcacatc gcttatgtaa tcattgttct tgtccttctc
     5941 ctgttggcat ggacgattgg tgatttatat aaaggaaaca aaaaatatga acagcttttg
     6001 caggaaacgg tagaagaaat cgaataaaaa ctgatttttc cacacaaaga cccttgatag
     6061 ttaggttaaa aatctagcta ccaagggtct ttttatttag aagataaagc ttttttcaga
     6121 tttaatttac tgaaaactag catgcttgtt actacgatga attgcgttaa tggcacgcct
     6181 gcaccagtgt agacacttaa aaaatagtcc tttttttctg gcgcataaga acaaaaccat
     6241 gagacaccga aaagaaaaac caattgtgtc agtattaatc cggtgaccac aatcaaagag
     6301 gaactataat aggcacgatc agcttttgca aatacggttc ctagtatcat aagtaatatc
     6361 cctgctattg aagcaatcag cacgggaata gccagaactt ggtaaatttt tatgatgaca
     6421 ttcgcgactt ttgctgattt tgttaattgg aaattaggtg ttctcggttc attactatcg
     6481 atccaattct ccattagcgg aaggtttaat aattcttgag cctgtttgac ttgttcttgc
     6541 gttccgaaac tataatttcc tccttgatgg aaatctttat atcgccatgc ataagcgtat
     6601 cctgattgaa ggaaatcgaa tacgattggc agatcactca tcgtctttcc atctcctgat
     6661 cccatcagat agaactcttt tttctttttg atggttccct tgtgataggc ttcttctaat
     6721 tcttggttga cttttttcca aaaatcattg gtttcttttg cattgttttg gtaataacca
     6781 gattcgctta ccacatctcg caatgcccaa aagatgatat ctccgggtaa ttcttttact
     6841 tttccacctt gagtccacgc gtgagtcgta tataattcgt caatacgggt agaaacagta
     6901 tggaatgtag gagatgcggc taatgcttta tttagtgcag catgtgagac ccatatctca
     6961 ctgttttctt tattcaagtt cgtttcatca tctattcgga tcagttgttt catcaaacgt
     7021 ccaaattctc cactggaacg gtcattgatg gttgcaatac cgtatgttgt ctcattttta
     7081 tattcgatcc actgatagac gatggaaaag ccaactatag gaagtaaaaa caatgaaagt
     7141 ttcagccatt cccgtttcga gagaagaaag agtcgttttt ttaacttctt tacttctttg
     7201 aaagaaaagt cttgccctag caagatctgg acgaaactga tacccaaagc tactacagta
     7261 aaaggaagaa gccagataga atcttcgcga atgaaccaaa agaaaggaaa agcgacagaa
     7321 agccctaaaa accatggcag caattttctg ctagaaccgt atttgcggta ataaatagct
     7381 aataaacaag cgataatgat ctcaacagcc ggaatcacga gggcattacg atatgttcga
     7441 gtggaatatt catgtgtaaa actaatcgga gaaaacaaaa aataaaaata gatacttaaa
     7501 agaagatact tgttcttcac aatcggttta aacgcgagtg caaccaatat actggcaaaa
     7561 atactgaaca tgccgaacaa aactgcataa gaaagatgca gtttatgtgc aagtactaaa
     7621 aataagctgt aggagattcc tttggaaagt gttgtagtcg tatactcccc tagccattta
     7681 ccatccaaaa tattttgagc atagtgaatc agcagctgat catcataggt cgccgacata
     7741 tctgttccat agattgcttt actatataaa tacaaccgaa taattgtaaa taatacggct
     7801 atgccaagaa taaagtatgt atgtttcttt tttagttggt cgatcaaggg gtttaaaatg
     7861 ttcagcatct tttcgtcctt tcaatcacgt tttgctactt gtttgtgaaa cttttcgttt
     7921 tttacttttc tatcagtata ccctgtttgt ctgaatttaa aaagaggggg caaaaggagc
     7981 atggcgaaaa cgaatgcaaa aagcactcct ttgtttctta aacaaaaaag gacaaggcag
     8041 actgcccaat ccttttttgt tgctgtcagg tagaaaactt aaagtgtgac ttcttctgct
     8101 aataactcag ggaacacttc gggatcgaat gcttgcgctt tgaacatgga aatttctttt
     8161 ccatatggtg cgtaagcttt ctttttatct ttaccgacat atggggtttc taatattttc
     8221 gaaatgttgt caaacattgg atcatggacg attttgttca atgtgtcaaa tccaattgtt
     8281 ccaaaaccga tattggcatg ccgatcttta tgagaacctt gtggattttt agaatcattg
     8341 atatgcagta ctttaagccg atctaatccg atgaccttat caaactcttc cattacacct
     8401 gaaaaatctt cttttatatt atatccggca tcattcgtat gacatgtatc aaaagtaacg
     8461 gataattttt cattcaattt tactccatcg atgatttgcg ctaattcctc aaatgttcga
     8521 ccgatttccg tacctttccc tgccatcgtc tccaaagcga tctgtgccgt ctgttctttt
     8581 gtcaaaactt cattcaaccc ctcaataatt ctagcaatcc cagcttctgc ccctgctcct
     8641 acatgagcgc ctgggtgaag ggtgatttga gtagcaccta aagcttccgc acggacaatc
     8701 tcttctctca gaaattgtac agcaaatgga aacatttctg gtttgatcgt atttcccaaa
     8761 ttaatgatat aaggcgcatg aacgacgata tttgttagtc cgttttctgc cataaatgct
     8821 tttccggcat caatattcat ctcttcgata gcttttcttc tagtattttg aggagcgcct
     8881 gtatagatca taaaggtcga agatccgtaa ctggctgctt cttctgccgc gcctaacagc
     8941 attttctttc cgctcatgct gacatgtgag cctaataaca tggatggatt cctccttgaa
     9001 aggcaattgt ttcgaacaat tcctgtatat tgataagatg atgtacaaag aaaatggttt
     9061 gtttttcttt gccctgttct ccatagtaac tgaaactttc cctaaagaaa agcttaagaa
     9121 ctctttcata aaaaagatca tttcctcacg ttttatctca ttcgaaaaag aaattcctgt
     9181 caaaaacagc cttgtctgtt tttttgtcct attatttgct ataattagcg aaataaaacc
     9241 attagttgag gtgacccttt tgccacgcaa aaatactcga aaaaaacaga atagaagaaa
     9301 aaaagaaaaa tggtttgttc ctaagattat ttttagagtg tttcaatcat tgactgtttt
     9361 tattactgtt cttcttttgt tgttcgcagc tctaggtatc ggaataggtg ctggctactt
     9421 tgcctatctt gtcgaagaca cacaattgcc gactaaaaaa gaactgcaga cagaactagg
     9481 aaacattacg gaaacgtcaa agattgttta tgcagataat acagagatat caaaaatcca
     9541 gacagatctg atgcggacga ctatttcatc tgataagatt tcgcctttat tgaaaaccgc
     9601 gattatcagt acagaagatg aatacttcga taaacatcaa ggttatgtcc ctaaagctgt
     9661 tttacgagca ctagtttcag aagcgactgg catcggctcg tctggaggat ccactttgac
     9721 acagcaatta gtcaaacaac agatcctaac cgatgaaaca acattcaaac gtaaagcaaa
     9781 cgagatcgtt ctttctgcac aagtagaaaa acacttttca aaaaatgaga tcattactac
     9841 gtatttgaat gtctcaccat ttggtcggaa taataagggg caaaatattg ctggagtcca
     9901 ggaagctgct agaggaatct ttggtgtaga tgcaaaggat gtgactctgc cacaagctgc
     9961 ttatatcgct ggtttgcctc aaagccctat cacgtatagt ccatacacga atacaggcgc
    10021 gctaaaagaa gatctttctg ccggtcttgc cagaaaagat tttgttttat tcagtatgta
    10081 ccgtgaagga caaattacga aagaacaata cgaagaagcg aaagcctatg atcttacaaa
    10141 agactttctc ccacaacaaa tcgcggaaca gactgacaga gaattcttgt attataccgt
    10201 gatgaacgaa gcaacaagga ttattgcaca acaattggca gaaaaagata atgctgatat
    10261 gtcagattca tcggtcagtg aagcttatta tcaaaaagca cagcaaacga tccaaaacaa
    10321 aggatatacg atccattcga cgatcgataa agatatctat gctgcattac aaaccggcgt
    10381 agaaaattat ggctacatgt tagatgatgg tgctggtagc caagtggaga ctggaaatgt
    10441 attgatggat aacagaaccg gacgtatcta tggcttcgtg ggtggtcgga attacagcca
    10501 gaatcaaaat aaccacgcct ttgatacaga acgtcaagct ggttcatcca tcaaaccggt
    10561 tcttgtttac ggtcctgcaa tcgatatggg acttgtcgga tcagaatccc gtgtatcaga
    10621 ttatgcaacg acttggcaag aaggaaaaaa tgctggtgag aaaatcgtca atgcgaccaa
    10681 tgaagggtcg aacaccttcc aaaccgtccg tgagtcactg gaatggtcca acaatatccc
    10741 agcttatcac ttatatcaag atgtgttgaa ttccggtgga tcgaaacaat acgcttacga
    10801 gcactacttg gcgaagatga actacccagc aaatgataat tggggcgttg aatcggctcc
    10861 tttagggaca gtagatgtaa cgacgcttca acagacaaat ggattccagg ccttagcaaa
    10921 tggtggagtc tacgaagaag gctatatcat tgattccatc acagataatg caggtaatgt
    10981 gatttataaa catgaaagta attctgtccg tatttattcc gaagcaacgg catcgatcat
    11041 gaatgacatg atgcgtagtg tgatcaatga aaaaatcact acaccattca aagatgtcat
    11101 ctcaagctta aacggaaatc taggaaatgc agattgggtc ggaaaaactg gttctaccaa
    11161 tgaataccga gattcatggc tgatcgtctc aactccatct atcactatca gtagttgggc
    11221 aggacacgat gacaatactg gaatggacag ccaagcaaga atacgttcgg ctaattatct
    11281 tgccaatcta atcaatcaag cttaccaagc aaaccctgag attttcggca ctgatgagaa
    11341 atttgactta tcatccgatg tgatgaagaa aaaagtatct gggttcacag gacaaacagc
    11401 tggaaaagtt actgtcaata aaaaatccat ccaaacgcca ggaaaaacag tggagtcact
    11461 ttgggcaaaa aatggtccga agaaaagcgc ctttaaattc ggcgtaggcg gtactgatga
    11521 aaactataca gattactgga acaccgcttc agcttatgct agagcgaatc cagagaccaa
    11581 agaatcagac gataaagatg attgatctaa tagattaatc agaattaaag ggagctgcga
    11641 caaaaatctt gtcacagctc cctttttccg aataaatggt gttcaaaagc ttacatctac
    11701 gatattaaga caaatttatg taaaaatgtg aaaaac
//
LOCUS       DACEDO010000084        11465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEDO010000084 DACEDO010000000
VERSION     DACEDO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.084"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..203
                     /locus_tag="IWO52_002421"
     CDS             <1..203
                     /locus_tag="IWO52_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356666.1"
                     /translation="QQSAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTY
                     TPATLSEAQARGLTPCSKCYGN"
     gene            complement(365..1537)
                     /locus_tag="IWO52_002422"
     CDS             complement(365..1537)
                     /locus_tag="IWO52_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0356667.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(1743..2558)
                     /locus_tag="IWO52_002423"
     CDS             complement(1743..2558)
                     /locus_tag="IWO52_002423"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0356668.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(2708..3154)
                     /locus_tag="IWO52_002424"
     CDS             complement(2708..3154)
                     /locus_tag="IWO52_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0356669.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3151..4155)
                     /locus_tag="IWO52_002425"
     CDS             complement(3151..4155)
                     /locus_tag="IWO52_002425"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0356670.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(4195..4524)
                     /locus_tag="IWO52_002426"
     CDS             complement(4195..4524)
                     /locus_tag="IWO52_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0356671.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(4650..6977)
                     /locus_tag="IWO52_002427"
     CDS             complement(4650..6977)
                     /locus_tag="IWO52_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0356672.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(7820..8113)
                     /locus_tag="IWO52_002428"
     CDS             complement(7820..8113)
                     /locus_tag="IWO52_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0356673.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(8516..9694)
                     /locus_tag="IWO52_002429"
     CDS             complement(8516..9694)
                     /locus_tag="IWO52_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0356674.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(9798..10112)
                     /locus_tag="IWO52_002430"
     CDS             complement(9798..10112)
                     /locus_tag="IWO52_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0356675.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10219..10434
                     /locus_tag="IWO52_002431"
     CDS             10219..10434
                     /locus_tag="IWO52_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356676.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(10734..10976)
                     /locus_tag="IWO52_002432"
     CDS             complement(10734..10976)
                     /locus_tag="IWO52_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0356677.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(10963..11391)
                     /locus_tag="IWO52_002433"
     CDS             complement(10963..11391)
                     /locus_tag="IWO52_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0356678.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         3249 a         2240 c         1973 g         4003 t
ORIGIN      
        1 gacagcaatc agcagctgaa caggcctctc agcaggcaca agccgcagct cctgagcaaa
       61 ataatgaaca aactgtctat gtcacaccga ccggttcaaa ataccataca cataaatgtg
      121 gaaatggtac gtatacccct gccactttaa gcgaagctca agctcgtggt ttaacccctt
      181 gctccaaatg ttatggaaac taattcaaaa gttatactat aataataaaa ttcttctgaa
      241 acataaaaaa atagaagatg gcgttccaga tagtaaacca aaaataacag tttacacgtc
      301 catttcgtca gcttctctct tttttcattt agtctgttcc ctctgctttt ttggacatct
      361 tcattcattc tttctcaatc aaatagtaca cctctttttc ttttcgcagt ttatatcttc
      421 cttttttcaa tttcattgaa aactgtttcg aatgtttatg ttttacatgg attttaagca
      481 ggagctcctc atgttgcaag cgatagttcc tgccttttaa atcagaaaat ctcactagtc
      541 cgtattcatc agaagctttc acaaatggaa ctttttctct agtaatatag tgtttgccgt
      601 tgagcgtttt tacagaaaag accattcctt cgattggtcg tccgtttgtg ttctttacat
      661 aaaaagataa aggatagttt cgcttagcga tggccaaaga atctaaataa gcgatgagta
      721 gccaaactaa gccgattgat ccaagtagta taccgataaa ccaaataaaa aatttccatt
      781 ttttccattg atcaattcct tggatcgctt tttttgcctc ttttgcttga taaggaatac
      841 gatgccccgt cacaagcagt cgatgtgtat tgaccatata aggagtacag gtaagcaaag
      901 tcacgagatc tctgcctttt tggataccca aagaatctac ttcatcaggt aagaccactt
      961 ggattttttc tacttgatac gccaatgtct ttccattgat ctggatgaaa aactggtctc
     1021 cttttttcaa cttaggcaaa tctgtaaata attttgcttc tgggagtcca cgatgcccag
     1081 aaagaacggc atgggtgttt ttcccgccag tcggataaga actttcttcc aataatgacg
     1141 ttcctttttg caaaaatatt tccgttgtct gatcgaatat aggtaagctg acattgattt
     1201 ttgggatttc gatgactcca agcatatgtt ccataaaaaa ggcattacta ggtaaatctt
     1261 ttaatgcttt tgcatcaact gcttggttga aactgcctat ccctggtgat acgttttgct
     1321 ctgccaactg ttggtttttc tttgtcattt tttcctgctg tttttttatt tcctctgaat
     1381 tcttattcga tgcttgcttt tgataatgga tgatcatttg ctgggcaaga aaatcattca
     1441 gtgaatcttc tacaaaagga taagaaaaga tcccgatccc aacgactaaa atgattgcca
     1501 tgaagatgtc cagtattctt attctgcttt tattcatcat gtccacctat tttctaatat
     1561 gtatatgaag aggttgtgaa atcagcgcct tgacctgagt attagcgaag caaacggcaa
     1621 aatagttcct cctattttgc cgtttgttga gcgaatacca agagaccgac cacttgtcac
     1681 aacctttctc cccatttgaa tcactatcgt ttatttatcg tatgattttt gtttccaata
     1741 gtttaagctt ctgtttcttg atggcgtttt ttgaaataga tcactgctcc tccaactaaa
     1801 gcagtaccta ccaagatgaa tacgatgatc cccataccac cagttgaagg caagattcct
     1861 ttaggttggt tatcgatcgt tgtttcatca gaagttgttg gatcatcatc tactgtaaat
     1921 tcagtgtacg cagcttcatt cttaggtaat gcgtagcctt ctggtgcttt tgtttcttga
     1981 aggcagtatg ccccttgttt cagcccttcg attgaaaatt tcccttctga atcagaaatc
     2041 aatttcactt cttcccagtc agaacctgtg acttcatctg accaagcata tgggtattct
     2101 cctgaggcaa ggtttgctat ttttttcatt aatttaacag ttggatcttt tgctcttaga
     2161 atagaaaatt ctgcatcggc taatttttct tgcgtgttca catcttgttt gatgaagtgg
     2221 aaatcaccgt agttttggac attttctaca gtaatcgtgt tatccgtagt tgtattttcc
     2281 cgattgacta caccgtttgg attcccaaga ttggcttcat ctgcgccatg gatgataccg
     2341 gttgtttgtt gcaattttcc gttttttact ataaaagtag tttctttttg tggattggtt
     2401 aattgataac catctggtgc ttttgtttca actaaagtat aattgccgtc tattaatcca
     2461 ttgacactga tggatccttc agtatcagtt gtgaatgtca acgctgactc agcaggatca
     2521 tagagagcga caccagtgtt ttcatcaaaa ccggtcacga attgattctc tgaatttttg
     2581 atctggaatt gcgcccctgc taacttatct acccctgaaa agttcccagc tttctggatt
     2641 ttgatattgc tttctttgat gacattttta gcatataggt gaatcgtgtc taaaggtttg
     2701 tcagggtcta gggtaagctt aacacgatat ttggcgacgt ttcgacaccg ggctttggtg
     2761 tttctacaaa gacgtataca gcatctttcc catcagattt acctggaaga gtcaggttag
     2821 ctaatccttc accagaagtc gttacacttg cgaagggtgt agcgtctgct ggaactgctg
     2881 tgtctttact tggatagtac gtagcagtga tgtcatatac gttgaattca acgccgttca
     2941 acggttcgct attcggaaat gtcaattccg ttccgttgtt tatttgattt tctggtgctg
     3001 aagtgaattt caatttatgc aaattaaccg taacattttc tggtgtaggt gatacggctg
     3061 cagaaacctc tgcagttcct aggccgagaa cgaaaggaag cagcatgata gcagttccta
     3121 aaaacgtcca tatttttgtt ttgatgtttt tcattttatt tccctcctat tgtttccttt
     3181 ttgttgtttt ttataaaacc aagctgtccc tgttgaaagg actagcaata accctaaaat
     3241 tgagaaacct ccaagcatct cttcgcttgt ttttggaaag cttcctccat ttttttcatt
     3301 tgatggacca tttgtctgca tctgaactgg cgtttttcca ttattcgtcg gatttgcttt
     3361 tggttcgttg atgatttcca atggtatagg atgaagcgga tcataactgc ctttttcaac
     3421 ttcaaagaga atggttgatt gactcaatat atatccttta ggcggtttaa tctccactag
     3481 ttcgtaactt ccaaaagaca atccatggac agaaaatgtt ccatctttac tggaagtaaa
     3541 aatcgttgct cttgaatcat ctttggctac ccaatgattt atcccgtttt cttgcgctaa
     3601 gtattggtca ttttttttga ctagaaattg agcatctgct agtttttgtt tcgtttgccg
     3661 atccactttg acaaattgtt tgcctcctgt ttggatggca agatgtgttt ctactgtttg
     3721 attgtcagtc gtcaaacgta catcgttatc aaatgtcgtt ttttcactgt tttctttcaa
     3781 tgtcatttgg taacggatcg ttaatttttt tccagcaaac gaactaagat cttttccaga
     3841 atctgataag acaaaaccat gttttatttt ttgtgtgtga aatgtatgaa tcttgtctcc
     3901 accaatcaaa aaatcgatcg tttctggttc tagccataac tgaggatcag ctgtatcttt
     3961 gatcgtatag gttcctatct cgttgatgtt ctcaggaatt gttacggtga tgagataagg
     4021 gactttatcc ccatctgcaa aatctgtctg cttattctct actgtttttg ttaatgtgag
     4081 tgcagcaggt attttttgtt cgctctttgg atacaagtgg atggtcgtca atttatgatt
     4141 agatgaatct gtcacaggaa gcacaacgac aaatggtgct gatttttctt ttacttaatc
     4201 tgaagtcttt atttccgtaa atcggtaaac agcgtcacga ccttgctcat ctttgtccga
     4261 tgcactaaaa gaagcaatgc cctcttcctc gtctactgtt tttgtcacat tttctgccag
     4321 tattttttca ctatcacttt tttgtgctag ttttctttgt gcgtcttcca cactacttcc
     4381 ttctgaccgc agcttgtaaa attcatccga gacatcatat atagcgaaag taacgccgtt
     4441 caaccctttt actccttgaa agttatccgg gatatttatt ccgtcgtttt gactgatact
     4501 tggcatctta tcgctttcga acattcgatt atgcaaagta aatgtaatag gtgattctct
     4561 agctgacaca gtcggagaac ctataaaaaa gaccagtagg ataaaaaaga gaacagcaaa
     4621 tatatccaca cgtacatttt ttgttttttt catcgctgtt cacctgcttt tttcttacga
     4681 tggataaaat aaaagaagcc agaaaaaata aagatcagtc ctaaaaagac aaagggcagt
     4741 agacctatcc cacctgtcga aggaagcaag agagtcggat tgatcgtcaa cttagcggta
     4801 tctgttgcga taatcgtata attattgttt acacttcctt cgcctaaact aatcgttact
     4861 atttgtgatc caatatctgt ccggctgtta actgttaggg tataatccaa tgattcgcct
     4921 aagacaacac cggtaacgtg gagatttcca accgaaatcg agtgactttg ggcatcataa
     4981 ttaaaggcag cgtcatcgac agtaatcact gcgttagctt tatagatggt ataagttcct
     5041 gatgtgttgg ctatatcagt aaatgacaac ttgggatttg ctgctttgac tttgtctatt
     5101 ccgctattcg ttagtttaat tgtatagctg ccagcattaa ccatattttt tgctgattgt
     5161 gcatctgctg tgtaatccat actgctaagt gccacttgta ctggagtaag cgaactagtg
     5221 acaccctctg ctagcttgac atggattgac gggacacctg cttttccatc atatgtctta
     5281 ctgccactga gctctaccgc accgctgatt tgcttaatcg tatagacatc agatgtctgc
     5341 acgatagtaa ataaatagtt tgttctggca gctagtttag ctagtccgct ttttgtcaat
     5401 tgccaagacg tataggttcc aatttcagta ggcgcagtta cttcaactcc cttttcattc
     5461 atcgctttga aatcagttgg attccatgtg atttgatcaa tactgagatc attgtttaaa
     5521 agggatgttg attctgtttt atcattaaag gcatatttaa cgatcggtac aacagaaaga
     5581 ccatcatagt acttactgcc atttgcattg attgatactg ttgcatttgt cttataatat
     5641 aggatgactt cagaaatctg ttttccattt gcgtccttta cgattacatt tccatttgta
     5701 tctaaactag ctaaaacact cattgctgat tggttctttg tttctatcgc tgaaacagct
     5761 gtaaaggtat atccgttgat agtaggcgta ttagtcgtca ggtcttgtgc agcacttccg
     5821 taagtaagat tcattatttt agaaggagca atcatctgtc caaaatagtc ttgatactta
     5881 atagtgattg gtgtttgttt gcctgtatag gtcaccgtat cgacgttact ttctagggct
     5941 gtgaaagaac cgtttgtttg tgcaaaagaa tagcccgtta ctgctgttgg agtgataacc
     6001 actttgtcac ccacatggat tccagataat gtagtcccgt acgttccgat ttttgctgct
     6061 ccaccattga ctgtataaga aaaagttcct gtccgattcg cattgacctg aaccgttgct
     6121 gcttgatagg ttggaaaatt tgaaacattt cccttaccat caacgatctg cccatttttg
     6181 atcgagtacc ccgtgaatgg aatatcattt gctaactgta taccgattga aaaagtactc
     6241 atacctcctc cggcaggaag ccctccccat gttgagggtg taaaagaata acttttatcc
     6301 gcattgggtg taactgaatc gatcgtattt ccagcggtag ccacccccat atagatactt
     6361 tttgggaatg tgatagtagt accatttact gtggagccaa taaacagatt atggatatct
     6421 ggacggttat caataaaagc gattgctttg gttacgcctt gtaacgcagt tttatccgca
     6481 ctcatcgatc cgttaaccgc aacaatgact ccatctttgg catatatcac tgtatatcca
     6541 tctggtatgg tatgtccgtt gaaattcgca gtggccgcaa cagtactaaa tgtctggctt
     6601 ctacctgttt ctgaataagc tttctcccct gctgcttttg ctgctgtgat ttctttttct
     6661 gtcggatcaa tcagttgagt cgaggatgtg gttgaggttg tctcgttgtt gctagactga
     6721 ttggtgcttt ctatggactg tatttcagaa tagggtggac tatttgtctc ctctgtcgtt
     6781 tggttagcaa aatttgacgt ttctgtataa acgtttacat aaatacttga taagctccct
     6841 aagaaagtaa aaatcaagat gatcgaaatc ataatgggat gtaagtattt tcttttagat
     6901 acagctttga gtctagaaac aaaatgtttt tgacatatac gttggatttt attttgagtt
     6961 ttcttaatca ttttcatccc tcctctcaat aaaaaatcat caaataatcc tttacacgtt
     7021 attctaaatt taatttttat tattttattg atgtatagaa agtttggggc tttttttctc
     7081 tttttattct aaggttattt tagtcttgtc ctttaataca aagattttat ttttaaataa
     7141 aaaatactct ttttttacga ttattgaaaa aaaaagataa aacttgatta attgaagtat
     7201 tgcttaaatt tggaagctat gagggaagaa taaaagagtt aggacgggat tatctactat
     7261 ttttctttgt tttaaatatt tgttatcttt gaacataaaa gagaaatgtg tcgactgcag
     7321 tgttgtgtcc ctcatcacga ataaacggtg ttcaactagc tgatcttgta ttcacttaaa
     7381 aaatggtaaa gtacgagttg ctattcccca ttttcaattt tctaacgctc aggacaaaat
     7441 actgttttca caacctcatt ggaataaaag catatatatt gtttcaagaa cgaaatcttg
     7501 ttatcttttt aaagggcatc tgtccaaaat ctaattttag gaggtacgtt ttggtattta
     7561 ttcgtacaaa taaaagagaa ttatctttat tcatgtaacg catccaattg attttttcat
     7621 gtacgctcct ggaacgattt tactgacgat tgcttattta actgcgaatc gctcattagc
     7681 ttttatcata gctatccata ttttgaataa cgttcttggc tttgtattat agatacagat
     7741 gacaaaaaaa gttcaagtat gcggtgtaga accaatatcg catacttgaa cttttttcaa
     7801 tgagtataaa ttaaaacttc taattttcaa ttcccatcat ctgatatgcc cgaccgtctg
     7861 aacttctttt gaaaaagcca aatgtgatta gataacgtct ttgttcgaca taatcgccac
     7921 tagaaaatac gttcagcagt atcgaattaa cctctgtttc tgtataagtc actccatggt
     7981 caaagcgttt caagacttct ttcaatagga tgtatttctt ttttgttgaa cttggtactt
     8041 gttttacttt tccatcattc atgaatgtgt ctaaaacttt catcttttct tgctccgtat
     8101 aagtataaac catcttcttt ctccttttga ttctaagaat actccattta tgttagaata
     8161 ttctggattt tttattaaag cggttactct taagaaagat attatcatag tgctcttttt
     8221 tttactacta cttataggta gatttattct aatccattat tctcccctac caataattaa
     8281 agaaaaaact ggatcagggt gttcaaatta catgctaaga aaaaagaacc cttgaatacc
     8341 aagggttctt tctcagtctg ataacaatga ttaacgacgg atttctttga tacttgtgaa
     8401 aagtcgttaa agtattgata taaaaggatt tttattacct ctgactacat attgactaat
     8461 ttgttaaaaa atcgtttttc aagaatcctg taataccagt gctttcaccg tttttttaac
     8521 tctctaaaga actaaaagca atcagaggtt ttaccttttc atttgacgtt aagtggcgat
     8581 aaattttcga agttgtttgg tcagtatggc ctaagcgatc tttcaagact tcccagctac
     8641 agtgttccgc tactaagctt gccattgtat gacgagctaa atgagtatga aatggcttat
     8701 taattcctga gcgattcacg caccgtttga cgtgatcatt tacatactct cgacgataag
     8761 gaacaccacg tttactagaa tcaagataca caaacaaata tgtttcttct ataataaacc
     8821 tgtaatgttg cctcatctca cccaaagcct taatcaactg ttgtagcttt tccataacta
     8881 gaggagttac tagaactcgt ctagcagaac taactgtctt agtagtttca attcgaaaat
     8941 ccttactctt acctttaact gccgtttgct tatagaacag aatttcattt cttttgaaat
     9001 ctatatctcc aggctgtaga gcaagcgcct cgctgactcg gcagccagtg taaaatagaa
     9061 catggaaaag tacagacatc ggtaattcct catgaacaat gccataattc aaaaaagttc
     9121 tcaattcatc gacagtaaga aacttgtcct caatattatt tatggcttct tttaaatcag
     9181 ttaccgttaa ctgatatttt ggcattctca cagacttcat tggattttca cttatcaaat
     9241 tctttctttg acaatattta aaaatcatat ttagaacttg aataagtgat tgtaaactat
     9301 ttttggcata gccaacattt gttccattaa cgccacattc ctttaaggta aaaagcaatt
     9361 cttgaaggca ctctggtgtc aaatcagaca actttttatc accaatccat cgaacaataa
     9421 tttttaattg ttcctctcgc ttatcatagg ttatttcctt ttgttcattt aacgaacggt
     9481 aatattggaa ccattgctca cctacaacac taaacttgtc attgcttgca acaatttttt
     9541 tcttaagcct ttgttgtttc tccatttcca gataagcgag ttgcgcctca cgttttgaat
     9601 taaagccacg tcttgttgtt cgatatgctt gaaagtacca tgtgatccta ccagtagaac
     9661 tgatttgctt cttgaatcga tctgtttttt ccatttattg attcctccta ttttgaagga
     9721 acccaacaag cagaaaccct ttggaaatct ttctatctat gtcatcattg taccgcatct
     9781 tagaaagaaa atgtaactta taaagatcga agtagtcgca atttttctcg atagtcattc
     9841 gaatcactct ttctcatcca ttctaaaaca tcatcacgaa aatagtgtcc tcgaatcccg
     9901 taccaacagg gaggaaactc accaagtttt actaaacggt tcagtgtact atcacttatc
     9961 tgaaatagtc gctttaaatc agtatttgtt aataccagag gtaaatgact gactgcaagc
    10021 aatttttccg tgtaatcttc cagttttttt tgtaaaattg aatagaactc tatttccaga
    10081 gaattaacat ttgttttaac ttccaactcc atcacccttc tctctcaaaa tcaaaacgac
    10141 caccacggta ccccttaaaa ttgtaaaaaa acactccagt actccatcaa aacggactag
    10201 aaaagaggaa tttttaatat gaaacaagga attgcagaca taaaaataat taaagaaatt
    10261 cttgaaaaat ctacggctaa tgcaatagca ttcggaacag gaatcaactt gagtacagta
    10321 aaaaaactta aaagtggaga gagagctgaa gaaaaattaa atcttgccga tgctattaaa
    10381 atcacagaat ttggcatgaa aaatatgcca accaaaatcg aaatatggaa atagtagttt
    10441 ttctgatcta tcagctgttt tgccaaagca cataggaatc tcacctcccc ccagtaatgg
    10501 cgggccgatt tggttacgga agtatcatta tcgtagtcgc gattctcata ttaaaatttg
    10561 tcgcttaaaa gctaaccttg tcatggcgta actagagaat caaaatctac atgtactgat
    10621 agatttgcta ttcatttgtc aaagagcgct aggctatttt cagcctatgg aaatccttta
    10681 ttttgacccg ataaaaaact tccatagaat gaaaaggaaa gggaaaaaat actttaacga
    10741 gttgtatcaa attcaagtat cttctcaatc aattttgttt ccagttgtct acgcatatat
    10801 tcatctacat acataaccat tcctttttct tcttcataaa ctggtttcat ggctaaccgt
    10861 gaaatataat tttgatattt tgacagaacg atggacatgg caattgaatc accagaagct
    10921 gcggctttga ttgtcgttag tggtatgaat ggtatatatc gtttattttg cttcatattt
    10981 gacacccaaa tactttccta gctcttccaa agccatttgt cgttggtacc aaacactgcc
    11041 gagacttttc ccaataatat cgccaatttc acgatcatta agaccgatga agaaggacat
    11101 aaaaatgatt tcttgtcttt gactaggtaa tgctgcaatc gcatttgcca actcctcatt
    11161 ggaaataaag attttcatac caaggacaat aaaagtacca ctactattca aatttggttc
    11221 caaataatag aactctgtac ctaacccttt ttgttccaat atttccattg atatttgccg
    11281 ttggcgaaat ctagcgtact tcttttgaat atttaacgcc tcattgcaga ctactttctt
    11341 acaaaaactg tcaaacgtat gttgaatgtg ggtttcctct gagtcgccca taatattctc
    11401 cccctcaaaa aattcattca actcgtttta cttctatccc tttccattat tactacaatc
    11461 tagga
//
LOCUS       DACEDO010000085        10980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEDO010000085 DACEDO010000000
VERSION     DACEDO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.085"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWO52_002434"
     CDS             949..1422
                     /locus_tag="IWO52_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0356679.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWO52_002435"
     CDS             1431..1658
                     /locus_tag="IWO52_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0356680.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IWO52_002436"
     CDS             2294..2665
                     /locus_tag="IWO52_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356681.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IWO52_002437"
     CDS             complement(2921..3163)
                     /locus_tag="IWO52_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0356682.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IWO52_002438"
     CDS             complement(3195..4097)
                     /locus_tag="IWO52_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0356683.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IWO52_002439"
     CDS             complement(4110..4298)
                     /locus_tag="IWO52_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0356684.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWO52_002440"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWO52_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0356685.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IWO52_002441"
     CDS             complement(4913..5806)
                     /locus_tag="IWO52_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0356686.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="IWO52_002442"
     CDS             complement(5884..6822)
                     /locus_tag="IWO52_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0356687.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IWO52_002443"
     CDS             complement(6856..7206)
                     /locus_tag="IWO52_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0356688.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IWO52_002444"
     CDS             complement(7239..8141)
                     /locus_tag="IWO52_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0356689.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IWO52_002445"
     CDS             complement(8134..8991)
                     /locus_tag="IWO52_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0356690.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IWO52_002446"
     CDS             9325..10134
                     /locus_tag="IWO52_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0356691.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IWO52_002447"
     CDS             10174..10671
                     /locus_tag="IWO52_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0356692.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         3216 a         2051 c         1841 g         3872 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
//
LOCUS       DACEDO010000086        10661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEDO010000086 DACEDO010000000
VERSION     DACEDO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.086"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            333..2279
                     /locus_tag="IWO52_002448"
     CDS             333..2279
                     /locus_tag="IWO52_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0356693.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2282..2737
                     /locus_tag="IWO52_002449"
     CDS             2282..2737
                     /locus_tag="IWO52_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0356694.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2751..4079
                     /locus_tag="IWO52_002450"
     CDS             2751..4079
                     /locus_tag="IWO52_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0356695.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4113..4397
                     /locus_tag="IWO52_002451"
     CDS             4113..4397
                     /locus_tag="IWO52_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0356696.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4399..4914
                     /locus_tag="IWO52_002452"
     CDS             4399..4914
                     /locus_tag="IWO52_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ0356697.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4930..5592
                     /gene="rpe"
                     /locus_tag="IWO52_002453"
     CDS             4930..5592
                     /gene="rpe"
                     /locus_tag="IWO52_002453"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0356698.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5599..6171
                     /locus_tag="IWO52_002454"
     CDS             5599..6171
                     /locus_tag="IWO52_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0356699.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6358..7878)
                     /locus_tag="IWO52_002455"
     CDS             complement(6358..7878)
                     /locus_tag="IWO52_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0356700.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8121..8306)
                     /locus_tag="IWO52_002456"
     CDS             complement(8121..8306)
                     /locus_tag="IWO52_002456"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ0356701.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8290..8472)
                     /locus_tag="IWO52_002457"
     CDS             complement(8290..8472)
                     /locus_tag="IWO52_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356702.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8713..8931
                     /locus_tag="IWO52_002458"
                     /pseudo
     CDS             8713..8931
                     /locus_tag="IWO52_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9426..10025
                     /locus_tag="IWO52_002459"
     CDS             9426..10025
                     /locus_tag="IWO52_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0356703.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10089..>10661
                     /locus_tag="IWO52_002460"
     CDS             10089..>10661
                     /locus_tag="IWO52_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356704.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3525 a         1674 c         1990 g         3472 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aattattaag tcgacaagaa gactttaaac ccgcttcttt ttttagtgat aaattggcag
      421 tgtcgaccaa gaccatttat caagatattg aattgttgga agattatgta gttaattacc
      481 aagtaacaat tgagcgatta ccttctttgg gtatcaaact cataggtgat aggaaaaacg
      541 ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa agatatttat tctccagaaa
      601 aaagacgtgt cgagatcatt aaagaagttt tgtttggtga taagcctgtt tctttaactc
      661 atttgtctca aagttttatg gttagtaaaa catcgttata caatgacctg agaattatca
      721 ataaaattat tggcagtgga gaagttaagt tgcagtcatc tgcagaagga attagtttta
      781 ctggtaatga acaagcaatt caacgagcaa tcaagcaact tgtattctat tatgcgaatg
      841 acatggaatc aaaaacattt tttcaagttt taaatatgtt gtttgattca aaagttatta
      901 gtgtcattca tgatttgcta ttcaatgatt acagtgaact gactgaaaag gtttctgatt
      961 actatgttcg gtcattgatt tctactttag ttatccaatg taaccgttta ctgaaaggtc
     1021 atcatttaac tgttgaagaa gaattcctct tcaatagcat tcgctatatg gaaacgtaca
     1081 ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt gaatcttcca ttttatgacg
     1141 cagataaaga atatctatgt cgtcaactat ttgcacatcg tgtgacaaat aatttaaaaa
     1201 ccagcaatga agagtacgct atgagcgttc ataaattgat tgaaagaatg agtgagattg
     1261 aaaaaattga tttgactcaa aatgatcgat tatatcaatc cttattgtat catgtgccag
     1321 caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa tccgttatta gaaaatatca
     1381 aatctcaata ttcagaactc tttagcatag tatggtacgc catgtccatc cttgaagaag
     1441 aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat tttcagattg
     1501 ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac ggtatttctt
     1561 ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat aatgtagaaa
     1621 tatcaacttt agataagttt catttagctg aaattgggga agttgatttg attatttcct
     1681 ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg gtaagtaaaa
     1741 cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa ccttcgcgat
     1801 ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct aaatttgttc
     1861 aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt ttggatttga
     1921 tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct tctatcttct
     1981 ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg catgcgaatc
     2041 catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg attaattggg
     2101 gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat gtatctgaga
     2161 ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaagaaatg gtcgatcgct
     2221 tgttacaagt tcatgatagc gaaaaattac taagaatttt tcaacaaaaa ggagaataaa
     2281 aatgtatcta gataaagaat tatgtttatt tcaattagaa gtgaaagatc aaaaagaatt
     2341 gtttcaagta atgtctgaac aattaaaaaa cgctggttgc gtgaaagata gtttcttaga
     2401 aggtatcacc aaccgtgaac aagaatttcc aactggatta gaagtaaatc aaattgggtt
     2461 tgctatccca catacagatt ccgcacatgt aaatagctct caaatctgtt ttgcctcatt
     2521 aaaggaacca ctggtatttt cagatatgac agataaatct catgaaatac ctgtacgttt
     2581 agtctttatg ttggcaatgt cacaacctca tgaacaaatt gatacactac aaaacttagt
     2641 tagcctattt caaaatgaag aaaaagtaaa tgaattactt gcatgtacca cgaaagaagc
     2701 atttattgag ttagtacatt ctgcaggagt ttattaggag gaatagagag atggatattg
     2761 tattaaatgc ctttgaatgg tttattggat tagggtcaaa cgtcttttta ccactgatta
     2821 tttttgtaat cgggatatta tttggattaa aacctggtaa agcatttgtt tccggcgtta
     2881 ctgttggtat cggttctatt ggtttaggat tagttttaag tttattatca gatggtttag
     2941 gaacagcaat tcaacaaatg ggggaacaat acggtggtgg cctgaatatc ctagatattg
     3001 gcgtaggtgt tggtggacct ttggcttact ctacatcatt gggaatttta atgattccaa
     3061 tttcattaat tcttaacttc atatttgtgg ttttgggttg gacaaaaaca ttgaatgttg
     3121 atatttggaa cttttggttc gcaattttcc taggtatgat tactcaatct gttacgggaa
     3181 acttttggtt tggcttaatt ggcgcgattg tcgcagtaat tttacaatgg ttcttagcag
     3241 atcgtacaca aaaaactgtt agcgaatttt tcggctatcc aggaatctct atcacacata
     3301 tgatgggatt atcaggagta ctatttgcca aaccagtaaa ttgggttttt gatcgtattc
     3361 ctggatttaa taaaatagat gcagatgcag aaacattaac aaaacgtttc ggtgttttag
     3421 gtgacacagt tgttattggc ctaattatcg gtattgtagt gggtattatg gcaggctatg
     3481 acgtagcagg aattggaaac ttaggaatta tgactgcggc agtgatgaag attatgccta
     3541 aaatggtagc aatgtttatg gaaggtttaa tgccaattgc agaagcggcg aaagaattta
     3601 cgaataaaaa gttaaatggt cgccaagtaa acattggaat ggatgcagcg ttaactgtgg
     3661 gtcatccaac tattatgtca acttctctat tgcttgtacc aatctcatta ttgctagcac
     3721 taattcttcc aggaaataaa gtattacctt ttggagattt agcattcttt gctttcgcca
     3781 tctgtttaat gattccttat tttaaaggaa atattgtgag atcattagta ggatgttcgt
     3841 tgtatgttgt tacaacatta tatatggcaa catggttggc accaattatc actgatgtgt
     3901 ttacattagc aagtttcgat gtggggacag caggattagt aactatgttg ttatgtggat
     3961 tatggccagt agggttgttt gttttattag catcttactt tggtgtaatt ggaataggtg
     4021 gattaggagt tgttttagta ggtgcttcta tctacatgaa caaagtgaag aaagcttaat
     4081 cataaaatta aaataagaaa gaagagatca atatgaaaaa attattagtt atttgtggag
     4141 caggtcatgc tacttctaca attgcagtag caaaattatc aaaatggttg gaagaagaag
     4201 gattaacaga tcaagtgaaa atttatcaat ctaaaattgc tgatgagcta aataaaattg
     4261 atgaatatga tgctgtagta agtacaacaa ttgtccctga tgtaattaaa gctaaagtaa
     4321 cgaatggctt gggcttatta acaggtattg gtacagatgc catttatgca gatgtaaaag
     4381 aaagattagg tgtttaatat gcgttttcat catgacactt tgactattga aacagtttca
     4441 gggcgtcctt cgtatcatga tattcgaaaa gaacttttag atacagttga tacagcaggt
     4501 attgagaatg gaatgttagt aatttcgagc ccgcatacaa cttgctcact cttttttgaa
     4561 gaaacgatgc atgacacaaa cttttttggt gatgattatt tacatgtaga catcaacaat
     4621 gtaatggaaa cgattattcc aaaaatgacg agtgaaaatc aatacaacag tccggggcca
     4681 aaacacattg attttggaac aagattatct gatcccaatt accctgcaga aaaatgggtc
     4741 atgctaaata ccgatgcaca tttacgttca tctatttatg gaaccaactc aatgacttta
     4801 attgttcaag aaggtaaact tttattagga gaaatgggcc gtgtctattt tgtcgattgg
     4861 gatcaactaa gagagcgaac tagaacaatc aatctcatga ttatgggaga ataaacgggg
     4921 gaatagagaa tgacaattgc tccatcattg ttaaatgcac ctatcgcaga agttaaagag
     4981 ttatttgaaa tcttaaaaga aaagaatgtt gattatttgc atattgatgt aatggatggc
     5041 tgttttgtac cagatatggc ttttggacca agtgttatta aagatattcg tccactaaca
     5101 gacgttgtgt ttgatgttca tttgatgatt gatacaccag aaaaaattgt cacaagattt
     5161 gtagaagcag gagcagatat tgttaccttc cacattgaag ctaccaaaca cgcaatgaag
     5221 gtcattcagt tagtcaagga agcaggagca aaagtaggga ttacttttaa tcctgcaaca
     5281 ccagtcagtt ttattgaacc agtattatct ttagtcgatc aagtattagt aatgacaatc
     5341 aatccaggaa cagagaataa gcattttatt caagagacag tagttaaaat cgaacagtta
     5401 gatgtaatac gcaaacaaaa tgattatacg tatgatattg aagtagatgg caagattgat
     5461 aatcaaacga ttaaagtttg ttctaaagca ggggcggata tttttgtatc aggcggtttc
     5521 atcttcaatg ggaaaaatac cccgcaagaa caaattgata aactaaatca atcattagag
     5581 gaagtgaagt aaaagatcat gaccaataaa gaccaaaata aaaactattt agaagcgatt
     5641 atcttagaat tacaaaataa ctcaaaacgt attaattctg tagagttaga acaatttagt
     5701 gagaaaattg ttcaagctaa acacatcttt ctagctggtg caggacgctc tggaattgcc
     5761 atgcaagcat ttacgaatcg cttaatgcat ttaggttttt cagtcagcct tgtcggtgaa
     5821 attagttcac cacattcgca acctggagat ttattaatta tctgttctgg ctcaggtgaa
     5881 acaggtagtt taaaaaattt agcggccaaa gcaaatcaaa gtggtgtaag cattgcttta
     5941 gttaccatga aaaaagactc gtcgattggt caattagcag attctgttct tgtattacca
     6001 ggtattacca aagatgaaaa tcaacgagaa gaagatactt tttcacaacc tatgggttca
     6061 agttttgaac aattagcatt tctcaccttt gatagtttag tgttatcttt aatggataaa
     6121 acaggtgaaa gctcagaaac gatgtttgct cgacatgcag attttgagta gtagatttta
     6181 aagataaaaa accattctct aaatgctaat tattcagcaa atatgagaat ggttttttta
     6241 tttgattaga cataacttaa taattcagta gttttgagcg taagcgtgtc atagaatggt
     6301 acctaaacac aaatagcccg atttgataca ctcaaatcag gttatttgtg gcttatatta
     6361 tttacagatt tcttgtatct gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt
     6421 catcgaagac agattgggaa tctctgtaaa aagatgattt aaatatttgc ggatgtttaa
     6481 cccattcgca acagctgtct ggataatact caatataatt ccatttgatc tagcgccttt
     6541 taagctttta gaaaatagcc agttttttcg accgatcact aactctttga tggcacgctc
     6601 tgctcggtta tttgaaagtt ccaagcgtcc atcaagcaac acaatctgaa gtccgtcttt
     6661 atgattgact gcgtattgaa ccgcctttcc aagctgcgat tttggcaaat aagcaaaact
     6721 ctcacaccaa tgaaaaaatt cagtcatcac aggctgcaac gtttccttcc tcgctacaaa
     6781 cctttcttct gcgctaagct tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa
     6841 gtctaatcct attttggcat gactttttgg ggacggttgt ttacccaatg cttcaaaaaa
     6901 ctttctgcga acatgcgccc aacaataaac attgtttacc ggcagctttc cataggccgc
     6961 gtaaccatca gtatgcaaga aaccgctaaa tgattctaaa aaactttttg gctcatcaac
     7021 ggaacgcgaa tttgcgtgct cgtagagcac aacttgtttt tcttcgcttt tacctgaagc
     7081 atataaccag taaaaggtct tttctttatg agtattcaat acccgatagc ttgtctcatc
     7141 cgcgtgtaag acctcctgat tgcttaattc ttgatgaagg caattgtaga ttggctctaa
     7201 aaccatttca cttaccttga tatgccaatt agtgattttt ttacgatcaa gagggaaacc
     7261 aagtgcatgc cattcttttt cttgtcgata tgccggcacc tttttaacaa atttttccca
     7321 aatactctgt gtcacaattg aagaggaacc tagactatta ttgatcaacg gtttaggaac
     7381 aggcgctttt ttgatttttg tttctcctaa tgcttcacat tctctgcaat gataggcatg
     7441 ttggtagatg acttgttttt ctatatgggc aggaacatac aatggctctt ctctaagcct
     7501 tgtcgcaccg atatcatgta aagtatgacc acagtcttca cacaaacaat cggcaccttt
     7561 taattcataa tgaataggaa cttcaggcag accttctaat gaaagtgctt tgagtccctt
     7621 gcgttttctg cgtttaaaag aagataccgt aatgggttcc tggctttctt gtccagtgtg
     7681 ctctggttca ttaaaagttg agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt
     7741 ttcttttttt cgtccaaaca agcgtcgatt catttcagca atttgttctc taagaagagt
     7801 attttcctgt tgctggattt caagcaactg tgttaattgc ttgatttgta caagaagttc
     7861 agattccatt tttgtcattt ttacaccacc attctagcga ttttcttaca tctattatac
     7921 cattttatga caacgaaaaa cagacaaaat ccttgattta acaaggtttt cgtctaatat
     7981 atcgcacctt ttttcgtttc ttttagcgta catttttctt cgattgaaag gccagtcagt
     8041 aagacttctt ttctggaacg cggccattga aggtacccac tttcaaatct tttgtacatt
     8101 aatacaaatc cattttgatc ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa
     8161 aataagaaaa gcgcttgatc gtaaacatca aggtcatatt cttctataac gaaagctgct
     8221 agtccatcaa ttccttttcg aagatctgtt tttccacaaa cgataaaaat atgtttcaat
     8281 gcagttgcat caatgagcat tggcattcaa ctcttttaac aaggtataca aaagatattt
     8341 atccattcca ttataaataa atatttcctt atcttccgat ttgattctgc aagctagttt
     8401 tggcttggtg tcaatttgcg atttttgagc cgcacttgaa aattgaactg gtacaatatc
     8461 ttttttattc attcgttttt ctccttctat atttgatagt ttaattatag aagaaagata
     8521 atcggcgtac gagatactat cctatgacac gcttacattt aatttaatat tttgcctcaa
     8581 attagccaca ctttttatag aactaagagg aaaaatgaag ttttcttcct attaatatag
     8641 ttgtttgaaa tcttaaatat agcgttttac gatgtaatgg gaagtatggt gaataaatca
     8701 actatgttcg tacgaagcat ttactcaact aatttaatcg aatctttcaa taaacaaatc
     8761 aagaaataca gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta
     8821 gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa
     8881 caggccgaag gcgagctcga acaaatgctg agtcaaccga tggagaatta gaaacaatct
     8941 acagtaggga agaaccattg acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat
     9001 aatgatgaaa tcatttagtt cttataaatt agaaaagaga aaataaatat tccatatcta
     9061 ctttattgga tccgtaacat gactaaattc tgaatacttt ttaaagctta tcatttggta
     9121 aaactgaata taagttaatt atatagactg aaaattttga attttcagaa tagaataact
     9181 taaaagtact gacaaaattt tttaaagggg caatattaat gttctgagat aatcaaatcc
     9241 taccgaaaat atcaaaaatc caatgtgaca caatcagatt gtgtctcatt cgagggaatc
     9301 acacaatccc acgtggctct aggcagaatt gaatttttgc ttggggcttt ttccttatat
     9361 tgattttgaa tgagacttat tatatgttat aatgcaatta agtatcattg gagagggcga
     9421 agaaaatgaa gacggtagaa cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa
     9481 tcttggcttc gggaaaatat ggccaacgaa atttggttct tttttcgatt gggatcaaca
     9541 ccgcctatcg catttctgat ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac
     9601 gcggtcgcgt ggtggtcaaa gatcgattag cgatgaagga acaaaaaaca gctaaacata
     9661 attcggtcta tatttccaac aaattgcgaa aaacgatttt ggagtacgtt caacaggaat
     9721 ttcctgatca actccttcag caagattttg acggttattt gttccctagt cgaaaaggga
     9781 cgaatgaacc gctcaaccgc caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa
     9841 tcggattgaa agacattggc ccacattcca tgcgaaaaac gttcggttac tttctctata
     9901 aacaggggac caaaacagaa atcattcagt ccttgttgaa ccattcttct cagcgcgaaa
     9961 cattgcggta tattggtatt acacaagaag acaaagatac agctgtaaaa agcttagatt
    10021 tataatataa aagttaagtt taaacatgga tagttttaaa ttagtagatt gagtgttggg
    10081 aggaaagaat gagtacatta tatattatag gaaacggttt tgatttaagt catggtttaa
    10141 aaactagata cactgatttt tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata
    10201 aaatagacac aatgataaat gaaaataatg aggtgagaag tacagctgat gataagatag
    10261 gttggtttaa ttttgaaagt agtttgcatg agttaattca aagtccagtc tttgaagatt
    10321 atgtagaaga gtctaaggat tatctcatag gttatggaca tgaagattgg tcagattctg
    10381 cccatcatga ttttcaaaat atggtttatg attatgttgt ttctctagaa gaattacaac
    10441 aacttttcaa ggaatggata cgcaaagaaa tacagccgac aatagaacat aaaattccaa
    10501 aataccaatt ttcgtccgat gtactattct ttacattcaa ttatacagat gttttggaaa
    10561 ttctatacaa gcaaccgaaa gaaaacatat atcatattca tggcagttat gacgatataa
    10621 taatcggcca ctccgagaat tttgacaact ttataggaga t
//
LOCUS       DACEDO010000087        10401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEDO010000087 DACEDO010000000
VERSION     DACEDO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.087"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            283..522
                     /locus_tag="IWO52_002461"
     CDS             283..522
                     /locus_tag="IWO52_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356705.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="IWO52_002462"
     CDS             complement(884..1144)
                     /locus_tag="IWO52_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356706.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="IWO52_002463"
     CDS             complement(1329..1826)
                     /locus_tag="IWO52_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0356707.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IWO52_002464"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IWO52_002464"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0356708.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IWO52_002465"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IWO52_002465"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0356709.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="IWO52_002466"
     CDS             complement(4548..5210)
                     /locus_tag="IWO52_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0356710.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="IWO52_002467"
     CDS             complement(5220..6035)
                     /locus_tag="IWO52_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0356711.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="IWO52_002468"
     CDS             complement(6297..6740)
                     /locus_tag="IWO52_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0356712.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="IWO52_002469"
     CDS             complement(6874..7212)
                     /locus_tag="IWO52_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0356713.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="IWO52_002470"
     CDS             complement(7200..7577)
                     /locus_tag="IWO52_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0356714.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="IWO52_002471"
     CDS             7801..8994
                     /locus_tag="IWO52_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0356715.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="IWO52_002472"
     CDS             complement(9160..9582)
                     /locus_tag="IWO52_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0356716.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9977..10171
                     /locus_tag="IWO52_002473"
     CDS             9977..10171
                     /locus_tag="IWO52_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356717.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10168..>10401
                     /locus_tag="IWO52_002474"
     CDS             10168..>10401
                     /locus_tag="IWO52_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356718.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQ"
BASE COUNT         3180 a         2058 c         1717 g         3446 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta ttgatatgaa
     9721 tcatttaatc gtttattata taaatgtcaa taatctaata ataaaataac gtacatattt
     9781 tattattgca tacttaataa cagaactata gcaaaaaatc ccgtgtatca acacgggatt
     9841 agattaagtt tcctcaatca ctattcataa cacaattatt ataacaagta agtaaacaat
     9901 tgaaaaggac atttattgaa aacggttgtt ttttacttaa ggaggaaatt tagcgaaaaa
     9961 attagttgca ttgtttttgg tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc
    10021 tactactcaa agcacaagtt cagatcaggc ttccagcagc agtaccacga tctccgaaag
    10081 tacggcagat tcatcgaaag aacaagaaca aattgaaaag aaaaaagaag agaaacaaaa
    10141 agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct
    10201 gaagcaaatc ctacaaaaga aaactatgca actgcttcag cagctatcca agcgctacct
    10261 gaaaacaaac aagagttgac aagccgtctt gctgcaattg attccacaat caaagcaaaa
    10321 gaagcagaag aagaacggaa aaagcaagaa gaaattgcac gacaacaagc agaacaagcg
    10381 caacaagaag caatcgcaca g
//
LOCUS       DACEDO010000088        10209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEDO010000088 DACEDO010000000
VERSION     DACEDO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.088"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            161..391
                     /locus_tag="IWO52_002475"
     CDS             161..391
                     /locus_tag="IWO52_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0356719.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IWO52_002476"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IWO52_002476"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0356720.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IWO52_002477"
     CDS             1802..1978
                     /locus_tag="IWO52_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0356721.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IWO52_002478"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IWO52_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0356722.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IWO52_002479"
     CDS             2593..3060
                     /locus_tag="IWO52_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0356723.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IWO52_002480"
     CDS             3145..4029
                     /locus_tag="IWO52_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0356724.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IWO52_002481"
     CDS             4146..4379
                     /locus_tag="IWO52_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0356725.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IWO52_002482"
     tRNA            4467..4547
                     /locus_tag="IWO52_002482"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IWO52_002483"
     tRNA            4554..4625
                     /locus_tag="IWO52_002483"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IWO52_002484"
     CDS             5140..5847
                     /locus_tag="IWO52_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0356726.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IWO52_002485"
     CDS             complement(5907..6551)
                     /locus_tag="IWO52_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0356727.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IWO52_002486"
     CDS             6678..6965
                     /locus_tag="IWO52_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356728.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IWO52_002487"
     CDS             7073..7393
                     /locus_tag="IWO52_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0356729.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="IWO52_002488"
     CDS             7474..8787
                     /locus_tag="IWO52_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0356730.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATIQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="IWO52_002489"
     CDS             8784..9503
                     /locus_tag="IWO52_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0356731.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="IWO52_002490"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="IWO52_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0356732.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3408 a         1682 c         2179 g         2940 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca caattcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaa
//
LOCUS       DACEDO010000089        10095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEDO010000089 DACEDO010000000
VERSION     DACEDO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.089"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO52_002491"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO52_002491"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0356733.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWO52_002492"
     CDS             complement(1172..1867)
                     /locus_tag="IWO52_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0356734.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWO52_002493"
     CDS             complement(1880..2596)
                     /locus_tag="IWO52_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0356735.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWO52_002494"
     CDS             2926..3534
                     /locus_tag="IWO52_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0356736.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWO52_002495"
     CDS             3859..4182
                     /locus_tag="IWO52_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0356737.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWO52_002496"
     CDS             4364..5734
                     /locus_tag="IWO52_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0356738.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWO52_002497"
     CDS             complement(5864..7165)
                     /locus_tag="IWO52_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0356739.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWO52_002498"
     CDS             complement(7519..9510)
                     /locus_tag="IWO52_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0356740.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWO52_002499"
     CDS             complement(9654..9920)
                     /locus_tag="IWO52_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0356741.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         2959 a         2096 c         1832 g         3208 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta acccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcg
//
LOCUS       DACEDO010000090         9439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEDO010000090 DACEDO010000000
VERSION     DACEDO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.090"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1294)
                     /gene="mgtE"
                     /locus_tag="IWO52_002500"
     CDS             complement(<1..1294)
                     /gene="mgtE"
                     /locus_tag="IWO52_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0356742.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITT"
     gene            complement(1312..2211)
                     /locus_tag="IWO52_002501"
     CDS             complement(1312..2211)
                     /locus_tag="IWO52_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0356743.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(2213..3010)
                     /locus_tag="IWO52_002502"
     CDS             complement(2213..3010)
                     /locus_tag="IWO52_002502"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0356744.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(2988..3692)
                     /locus_tag="IWO52_002503"
     CDS             complement(2988..3692)
                     /locus_tag="IWO52_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0356745.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            3823..4398
                     /locus_tag="IWO52_002504"
     CDS             3823..4398
                     /locus_tag="IWO52_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0356746.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            4516..5193
                     /locus_tag="IWO52_002505"
     CDS             4516..5193
                     /locus_tag="IWO52_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0356747.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(5272..7080)
                     /gene="pepF"
                     /locus_tag="IWO52_002506"
     CDS             complement(5272..7080)
                     /gene="pepF"
                     /locus_tag="IWO52_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0356748.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(7256..8254)
                     /locus_tag="IWO52_002507"
     CDS             complement(7256..8254)
                     /locus_tag="IWO52_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0356749.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(8480..9133)
                     /locus_tag="IWO52_002508"
     CDS             complement(8480..9133)
                     /locus_tag="IWO52_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0356750.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         2781 a         2052 c         1578 g         3028 t
ORIGIN      
        1 aagtcgtgat aaagggaccg cttgctactg ctggatcaaa tcctagacga tccattagca
       61 tcgggattaa actacctgca agattcgcca ctgtgattgc aaatagcata gccatcccta
      121 tgacaaaccc caagataaaa ttatgtttcc aaaaacctac gattagaaaa atcgtgatcc
      181 ccgtaacagc tcctgtgaca acgccggtca agacttcagc caaaacaagt cgaacaaatc
      241 catgatcttt ctcatcagaa atcgcaagtc gacgaacagc cacagctagc gactgggttc
      301 cagcattacc ggcagttcct gtaatcagcg aaatgaagac tgctaaaata ctcgcttcac
      361 ttaccaagtc ttcatagtga ctgattaaag aagcggtcga catacctaaa aacaacagag
      421 tgatcagcca aggcaaacgt cgagacgctg cctttactgg attttcatta atttcttcca
      481 cattgacccc ggcaagtcct gaataatcgc tagcagcttc atcatcaata acgtcgatga
      541 tatcatcgac tgtgacgatt cctaacaaat gatcatcata atcggtaaca ggaagagcta
      601 agaagtcata atctctaaat gtttgtgcca catcttcttg gtcgtcgcct acatgaactg
      661 aaagcactcg ttcgctcatc agatcagaaa tcattgcgtc gtcatcattc acgattaggt
      721 cgcgcagaga aataacccct actagctgac cttcttgatt aacgacatag atgtaataga
      781 ttgtttcagc tacatctgct tcgttcttca gcacgtacat cgcagagcga acagtttgat
      841 tggctacgat cgaaacaaat tcagttgtca tgatcgcgcc agccgtttca tcctcgtaat
      901 ggagaagttc cttgatttca ctggcatcgt ctgccgacat caaacttaaa tatttagcaa
      961 tttgcttttt atccagcgta ttaagcaagt caacagcatt atctgtatac atctcagaaa
     1021 gcatatcagc tgcatagcta ggacgcattt catctaggta ttctttcata tgttcgttgt
     1081 cttcttcaat cacatcgaac atatccgcta attctttagg cgacagatag gaatagatca
     1141 gctgcctgtt ctcttctgta agagattggt agaattgccc ctgctcatag atatgcatct
     1201 ctaaaaaact gtttctgaat gcctgaatat tattttccct taaatcttga gtcaactggg
     1261 caaatctttc ctctaattct tgattttcat ccaaaccaat ctcacctcgc cttattcttc
     1321 aaaccgtctc acaactgcct ccatgtctgc ggctaaaggc tgcttgaact ccagcaactc
     1381 ttctgtaaat ggatggtaaa aacgcagatc ataacaatga agggcttgtc tttttattcc
     1441 cagatccatt cgcccgccat acatatcatc accgagcagg gcacagccaa ttgcggaaaa
     1501 atgaactctt atttgatgtg tccttcctgt atgcagctgg atatcaacta acgcgatttc
     1561 tgttagtcgc ttctctatcc agtactcagt ctgtgccgaa cgtccttcat cagagaccat
     1621 tcgcttcaat agcgaagaat aatctcgggc gatcggcaaa tcgatatgtc catgtgccga
     1681 taaatctgaa agccttccac tgactaaggc ctgatatttt ttcacaaact tcttttcacg
     1741 aagttgcata tctaattttg cgtgcgcaaa tccatgcttt gcaaaaagca tcaaaccaga
     1801 tgtatcccga tctagtcgcg tcaccacatg gattacttgg ttttcgtaac cttgtttttt
     1861 ataataagcc tttacacgat tagccattgt cccatttgga tggtattggg caggaataga
     1921 cgaaacgcct gctggtttat ttactaccag aacgtgttca tcttcataca cgatatcgat
     1981 cggtgtttca tccaaaagta cggtttcatg ttctccttca gcaggaatca caatggttac
     2041 cttatcatcc ttagctagag aaaacaagac attttctact tgatcattaa ctttgatttg
     2101 accgccttga aattttattt tcgctaacag accctttgag atcccttttt cttttaaaaa
     2161 atattttact tgctgagaat gatctttctc ataatcccat gtaaactgca tatcaatcct
     2221 cactaataaa ggcatctttc acacgatgcc aaaaatgcat atgtcgataa gaagcaaaat
     2281 ggatccgttc ttctgctatt cgataataaa ttgagcggat atttgctttt gcgacattca
     2341 attgatcaac cgttacaaga taatcttctg tattttccaa ttttatttct agccattcat
     2401 catgggcaat cacgatcgga gaacccaaag tacgaaacac tcgattattc agtgaagcaa
     2461 tctctgccaa ctgaaaggca ttgatactag gatggatcac agcaccgccg acacttttat
     2521 tatatgctgt cgaaccagta ggcgtagaaa tcgtcaatcc atctcctcta aaactttcaa
     2581 acagctcgtt tttgatgtat acatcggcta ccatcgtacg attggcccgt ttaatcgttg
     2641 attcattcag tgctaaaaaa tgctggtccg gtgtttcatc caaataactg atccgaacat
     2701 ccagcaaggg ataactaaca ctttgttccc ggttcgttcg taagctgtct actagttctt
     2761 ccagctcgta atcccgccaa tccgtgtaaa accctaaatg acctgtatgc acgcccaaga
     2821 aacgcacatc attcagacaa tgactgtagt gatggaacgc cgacaataat gtgccgtctc
     2881 cgccgactga gatgaccaat tctggctcat gatcatctcg ttgaatccct gcttgttcta
     2941 aaagtgcgtt caaacgttct gtcacttcta tagtctttgc ttctttatta tgaacgatcg
     3001 ctactttcat cccctgcctc ctctctttcg ctttctttct tcaaaagcgt tcgatagtag
     3061 acatcatctg attttcctct tccatgggag aataaccgct gtgcttcttg gatttcttca
     3121 cggatactgg acatctcttc atccaactga taggcagctt ctgccgcacg ctgaagtcgt
     3181 tcactcattt cttctggaaa aacgccttga tatttataat tcagcgaatg ctcaatcgtt
     3241 gcccagaaat tcatcgcaag agtccgaatc tggatttccg ccaaaatttt cttctcaccg
     3301 ctgatcagct gtacaggata ctcgatcacc agatgataag aacggtagcc acttgctttt
     3361 ttatttgtga tgtaatctcg ttcttggaga actttcatgt cttttctttt acggatgatc
     3421 tcaacaactt gatggatatc ttctacaaac tgacacatga tccttaaccc ggcaatatca
     3481 gacatttctt cttctaaacg tcccattgga atatgccgta attgtgattt tgttacaata
     3541 ctatcgactg gcttgactct cccagtgaca aattcaatcg ggacatgtcg attttgttca
     3601 cggaactgtt tgcggatccc ccgcagtttc actttcaatt ctgatacagt ttgttcatat
     3661 ggtgctaaaa atgcttccca atctcgttcc atagctgata cctctacttc attaacattc
     3721 attgtttatt ctaccatata tgcaaagttt tttcgtactc ttgatttatg cattgaccaa
     3781 aaaaagaaaa ttaggcaaaa tagacaaaaa ggagtcgatc aaatgacaca aatgcttgaa
     3841 atcgaatata aatctatgct gacaaagcaa gagtacgaga tattcatcgc tcactatcaa
     3901 ttaacagata aagatttccg tgtacagact aatatttatt tcgataccgt tgatgaacag
     3961 ctgaagaaac aaaattgtgg cctacggatc cgtttcgttg gtcatcaagc tgaatataca
     4021 ttaaaaacac ctgctgaaaa agggcgtctc gaaacaacgg atacctttat aacagaagaa
     4081 gcagaagcat ttatcttcga aaaacgtctg cctcgttcag gcgcagtttt tcaaaaactt
     4141 tctgatttga atatcgatcc tgcttcatta atccaaaccg gaaagcttac aacaaaacgg
     4201 gctgaattcc ctattgaaga aggactttta gctattgatg aaagctgggg agacaatcta
     4261 cacgattttg aactggaact agaggtaggg gatgcatctg tgggaaaaat tgcttttata
     4321 aattttttaa aacgtttttc tttaccttat cgtcctgcta agaacaagat ccaacgtatg
     4381 cttgaagcta aaaattagaa acctttcatt ttcacaaggc acttgagttc atttttttac
     4441 ttttttctgt taagttatag gatgtgaaag aatctagtca ctagttgtat agaatctatt
     4501 ttgggggaga ttcatatgat cgaaatctat ttatttgtta atcctttagg ggaatactgt
     4561 ttcgaaatgg aacaacagtt attacaattt atcgaagaag agtacgggac accatcaaaa
     4621 gaaaagatgc aatttcgctt tcttcctttg gtcaatttgc agacaattgg ggatgtcatg
     4681 caacaaaaag gaatttctca gaacaatctt gaggaacgta atcacttatt ctcaatgact
     4741 tattctgcag cattggactg caaagcggcg cagtttcaag gaaagaaaaa aggccgccgt
     4801 tttttgatca aattgcaaga agcagtcggc tgtaatgaca taccatattc tcaagaactt
     4861 gctgaatcca tatttgaaga aatcggcggc gatttgaata tgtttaaaga agatcgtcag
     4921 tctgacttcg ttaaagaagt attcttttct gatcaaaaaa ttgctcggga aatgggcatc
     4981 acaaaacacc cttctgcagt cgtttacaat tatgcctgcg accgtgattt tggtgtttta
     5041 gtagaagtaa aaggtacaag tagcctttgg agaatcacag agctgtgtaa aacaacagat
     5101 aactcatatc aagtttttaa tggtgaacat ttacatacaa atgattctca gttccattca
     5161 agaactagcc atttacactt gctttctaat taaaaaaata cgtcgactcc cttacaagag
     5221 agccgacgta tttttttcgt ttctattcga gaaaatcaaa aatagaaagc cttataaacg
     5281 atcgatcaat tcttctaatt catttagacg ttgttcaaac atcgacattg catcttcaat
     5341 atatgcagcc tgtgtcatat caactcctgc ttttttcatt acttccacag gataatcgct
     5401 gttacctgct tttaggtaag ccaagtagtt ctctagtgcc tctggttcct ggtttagtat
     5461 tttcttcgcc agtgctgaag cagcagaaaa gccagtcgag tattggaaaa catagtaatt
     5521 ataataaaaa tgcggtatcc gtgaccattc gaatttaatt tctgggtctt cttcgactgc
     5581 tggaccataa tattttgcat tcaatttacc ataactatca cttaggtatt cactagtcaa
     5641 tggcacacct ttttcatctt ccgtatgcat gaaatgctcg aattcagcga attgtgtctg
     5701 gcggaaaact gttcctttaa acccatccaa gtaatggttg agtacataag cccgtacacg
     5761 agggtctttt tctgtttcca ataaatactc cgttaggata ttttcattcg ttgtcgaagc
     5821 aatttctgct aaaaagatgg agtagtcgcc gtacacataa ggctggttcg aacgagtgaa
     5881 ataactatga acactatgtc ccatttcgtg gacaagcgta aatagctgat ccagcgtatc
     5941 atgccaattc aataaaatat atggatttgt gtcatagctt cccgaagaat aagcatcgct
     6001 tcgtttccct ttattttcga caacatcgat ccaacgttca gagaatgctt tttctacgat
     6061 ggccatgtat tcttcaccca ttggtttcag tgcttctaaa gctttttctt ttgcttcttc
     6121 gtacgtaaag gtaattggag cttcccctaa gaccggtgta taaagatcat acatgtgcag
     6181 tttttccact tctaataaac gtttccgtaa ttccatgtat cgatgtaaca aaggcaaatg
     6241 cttgtttacc acgtctacca aagtatcgta tacactttca ggaatatgat tattgctcaa
     6301 agacgcttct ctggcagagc tgtaattacg gactttcgct ttaaaattat gtccttttat
     6361 atgtgtgcct aaagtagaag caaatgtatt tctaaattgt tcgtaaacac tgtacaatcc
     6421 cttaaatgct gcttcgcgca cccttcgatc cgtgctttct agcaactgac catacacgcc
     6481 atgagataat tggactattt caccattttc tccttcaatc gttggaaaaa ctagatctgc
     6541 attattcaaa acagcaaatg tatcacttga agcatcaaag atttcacctg ctccagcaag
     6601 aagagattcc tgctcagcag ataagacatg ggctcgatta tctactattt gctggatata
     6661 atggcgatag acttccaatt ttggttcttc tttgaaatat tgccaaatct ggtcatctga
     6721 caattgcaat atttctggtt caaaccagga aacggcttca ctaactttcg aaaacagact
     6781 gcttgctctt gcataaagcg cttggtaatc tgtatttcca gtgtcttgat cgtttttaag
     6841 gtgcgcatat acataaatga cttcagtttg gcgataaacc ctcaatacga attcaatcgc
     6901 atttaaaaat tgagaagcgc cttgatcaag tgttcctttg tgtttttcag attgttttaa
     6961 ctcttctgat aattccaaat atttctcatc aaactcttgg tcgcttgaaa agatcttggt
     7021 caagtcccaa gttaaatttt caggcaattc ttctcgtttt ggcaactgct ttacttccat
     7081 gaaatccact ccttttcttc ccttagctct atctatcaaa atagatcata gtctaacatc
     7141 cacatgtcac agcatctaac ttttctactc attcatgtgt ctgcttccat caattttagc
     7201 ataactaaat cttgttttac atagaaaacc gattcctagc ctatttttat acttactatt
     7261 ttttcaaaga agacaagtgt atgcattcgt taaaaaactg ctgatagacc atctcttcat
     7321 caatcatagg aaaaagccat tcccttttat gttcttttat ttggcaaagc catgagttga
     7381 aatcaggaac tttctggttt tccaaggctt cttcgtacaa cattcgataa agaaaaacct
     7441 gttcttgtaa ataaaagaaa aaccgacttt tttgatagat ccacgatgat agatgcaaaa
     7501 gatgtttatg acgcaagtag cattgttctt gtatggatac tgtattcttt tgcttgtgaa
     7561 taagttggcg tgtcagccat ttcttcccat cttcactcac tttttctatt tttttcattt
     7621 ggttcatttt aatttcgtta ctagtgaata tttctttaag gtccctacta gaaaaagtcc
     7681 agcagctact gcaaaaggaa atatgccctg atacagtctc tgtaatatga tgatacaaat
     7741 aaagcttttg tttcttgaga tcagctttcc agcaatgcac tcctcttttt ctattatagt
     7801 agcagcaact tttttctatc aatgagaatt gcgattgtcc aagaaaagaa ttaccaagta
     7861 tccaccatac ttggtaactg ttattcaaat aattttgggt tctttcaata aatctctggt
     7921 gagacagtct agaacattgg atttctattg ctagattcgc caacaaaata tccggccttt
     7981 gccgcaacct cggcaaatag gcttccaaaa atactggttc tactgactgt cccagccaat
     8041 ccatgaagca ttcctttaac tgtaagtgct cttttgattc tctttcacta aagaccgaac
     8101 agttatctga tttttgatgg gcaaaatgag cacatttttt ctttcccttt ttcaaaatca
     8161 ctctttttcg acagccacga caataatatt tctttccttt ttctgcatgt ttagcaaaac
     8221 agcacttatt atattcattc attgcaagca gcatttttct ccctctgaac ttatttacgt
     8281 ctaagaattt atttacacga aaaaagaggg cgtgaaaaag ctattctgca tcaagtaata
     8341 agaacagcta aacaaaaata gttcccaata tttttcgttt ggttgggctt gttaccgcag
     8401 atgcaagctt ttgaacacat ttatacgaaa aaaagactgc gacacaattt ttgtcacagt
     8461 ctcttactat aatgctgatt tatacaaaat aaaatttcgt tcgctcgata gcattacgcg
     8521 ccatcagaca ttgtccatat tcattcaatg tttctggtgt acgatcgctc ttttcagcaa
     8581 actccaaaat tcgagcaatc tgattctcta catccgcttg gttgagattt tccatccaga
     8641 agtgaacgac taggtaatag cgctcttcga ataaatacaa atcagtccaa gcagactcta
     8701 gttcgacatc tgttgcaagt tggatcactt gttcaaaatc ttcaaaagca aaagttgctt
     8761 gtctttcctg ttcttctagg gcgcttccag ataattcttc ctccggatcg ctgccaataa
     8821 tctgctgacg aagaaagtca ctcacatctt ctgaatcaac agtagaatct tctgattgag
     8881 aaatttcttc atccggaaga tttttactga taaacagttc taatccgtct ccttttggca
     8941 aaacttggaa agtgacagct tcgcttcctc ggaactcctc ttcgatatca acttcttcca
     9001 aaatactata aaagaaattt tcgatttctt tgtgattgcc aagtaaatcc aaaaatgaaa
     9061 cgccccgttc ctctaaatct tcatgaccaa ttagtacacg gatcgtattt tcattaatat
     9121 gttccatttc cattttgcta cacctcactt tgccacaatg tttaatatag attcattgta
     9181 gcgaaatcag cttaaatgta aagaaaaaaa atctcgctgt taagcgaatt taataaaacc
     9241 agcggatttt tcaacctttc aagctatctt atttaactat aacatttttg gcagacgaat
     9301 actatatagc cgacttttca gctaattttc cctctctatt tttcgtgcaa tcatcatctt
     9361 ctactacctt tttatacatc taatcaagaa tactttttgt aaaaacaaaa aaacttggaa
     9421 aaactatctt tgtaatagt
//
LOCUS       DACEDO010000091         9044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEDO010000091 DACEDO010000000
VERSION     DACEDO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.091"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..260
                     /locus_tag="IWO52_002509"
     CDS             51..260
                     /locus_tag="IWO52_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356751.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IWO52_002510"
                     /pseudo
     CDS             467..667
                     /locus_tag="IWO52_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="IWO52_002511"
     CDS             complement(1379..1984)
                     /locus_tag="IWO52_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0356752.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IWO52_002512"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IWO52_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IWO52_002513"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IWO52_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IWO52_002514"
     CDS             3019..3990
                     /locus_tag="IWO52_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0356753.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IWO52_002515"
     CDS             4130..5443
                     /locus_tag="IWO52_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0356754.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IWO52_002516"
     CDS             5486..6352
                     /locus_tag="IWO52_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0356755.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IWO52_002517"
     CDS             6349..7197
                     /locus_tag="IWO52_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0356756.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IWO52_002518"
     CDS             7211..8683
                     /locus_tag="IWO52_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0356757.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>9044
                     /locus_tag="IWO52_002519"
     CDS             8700..>9044
                     /locus_tag="IWO52_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0356758.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEY"
BASE COUNT         2859 a         1534 c         1855 g         2796 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtat
//
LOCUS       DACEDO010000092         8732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEDO010000092 DACEDO010000000
VERSION     DACEDO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.093"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..74
                     /locus_tag="IWO52_002520"
     tRNA            2..74
                     /locus_tag="IWO52_002520"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IWO52_002521"
     tRNA            78..150
                     /locus_tag="IWO52_002521"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IWO52_002522"
     tRNA            155..225
                     /locus_tag="IWO52_002522"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IWO52_002523"
     tRNA            238..311
                     /locus_tag="IWO52_002523"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IWO52_002524"
     tRNA            323..394
                     /locus_tag="IWO52_002524"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IWO52_002525"
     CDS             660..1853
                     /locus_tag="IWO52_002525"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0356759.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3456
                     /locus_tag="IWO52_002526"
                     /pseudo
     CDS             1983..3456
                     /locus_tag="IWO52_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3539..4327
                     /locus_tag="IWO52_002527"
     CDS             3539..4327
                     /locus_tag="IWO52_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0356760.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4394..5062)
                     /locus_tag="IWO52_002528"
     CDS             complement(4394..5062)
                     /locus_tag="IWO52_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0356761.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5177..6889
                     /locus_tag="IWO52_002529"
     CDS             5177..6889
                     /locus_tag="IWO52_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356762.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6892..8664
                     /locus_tag="IWO52_002530"
     CDS             6892..8664
                     /locus_tag="IWO52_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0356763.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1945 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatgggccat gcttgtctca ttgctgttag ggttccaatt attaggagac
     2641 gaaggaatga gtccgcttgt ttctgctttg ctgctaggta gtatcgtaat gttaggtatt
     2701 tttgtttttg ccgaaaaaag agctaaagat cctgtcatca tgatgcattt attcaaaaat
     2761 ccactatttg ttgtggtcaa cttggctgca gcattggcta gtggtttttt gatgggaatc
     2821 gatgtttata tccctatgtg gatgcaaggt gtattagggc tgaatgcagc aattggtggg
     2881 ctggtattag caccattatc actagtctgg atggtcggtt ctttccttgc aagcaagtgg
     2941 atgactcaaa tgaaaactca ttgggtcttg agaattggac tactaatcgc agctattggg
     3001 ggcgcttggt tgtatcttat gcctttcaga agtaactatt tatggttttt tgctatatca
     3061 acggtcatcg gtataggctt aggtgtgacc atgactacct cgactgtcag tgcacaaaat
     3121 agtgtatctc gtgaccagct tggtgtagca acttctttta atacgttagt tcgaacaatt
     3181 ggtcaaacag tcatggtttc tatttttggt ttattgataa atggggtaac taaacgagaa
     3241 cttgcagcag caaatttaac aaatgatgca gatatcatga ataaactggt gaatccacaa
     3301 acggctaaat atattgatgc ggatattttg ttaccattaa gaaaaattct ttatgatggg
     3361 ttacattccg tttatctagt tggcttgtta ctcgtgatca ttgctttggt cttaacgctt
     3421 ttcgttcatg aaaaaagtgg taaaataaaa cagtagtttc ggttaagata tgtacgtagc
     3481 cgaatcattc aaatttaaag ttattcaaaa ataacttctt actaaggaag gcgaaaatat
     3541 ggcatttatt caagcaaata tttattcgaa tgtgttagaa atggaagtca atctaaatgt
     3601 gatactaccg caaacaacaa aaaaggtgat tggcaccagt tcagagcaag agacaacgga
     3661 tatacctgtt ttatatttac ttcatggaat gggtggcaat catagtgtat gggaaagacg
     3721 tacgtcgatt gaacgttatg tagcaaacta cggattagct gtcatcatgc catctactga
     3781 tcttggctgg tacacagata cagtttatga catgaagtat tggacattca ttgcagaaga
     3841 gctacctgag atttgtcatg aattattccc gcaattaaca agaaaaagag aaaaaacata
     3901 tgccgctgga ttatcgatgg gcggatatgg cgcgttaaaa ctaggccttg caaaatcaga
     3961 aaatttcgca gcagtaatct ctctttcagg cgctgtttct gtaggaaatc gaatggatga
     4021 tcttttgatg gttcgtaaag catctttttg ggaagggatc tttggaccat tggatcaaat
     4081 cgaaggttca aaaaatgatc cgcaatactt attgaaagaa ctagtaaaaa gcggaaaacc
     4141 agttccaaga ttttatatgg cttgtgggga atctgatttt ctttatcagg caaatcagga
     4201 aatggtatct gctatgcagg aacaaggctt ggcggtcacc tttgaacaag gacctggcga
     4261 tcataactgg atcttctggg accaatggat tcaacgtgca ttggcgtggc taatggaaga
     4321 aaaataaaaa aagagtctta aaaagatagc tcctatagag atcacaaaca tagtgatctc
     4381 tataggagct tttttactgc tcgttttctc ttcttttatt ttgttggcat aagccttcaa
     4441 tataggtttg tgcactcgtt agaaagagtg ctttttcatt caattctgca tgggactgca
     4501 tactttcttt aaatccctct aattgatttt caactacata attacgcata aatagaactg
     4561 tttctttcaa aaattcattt ccgtccataa ttttttgtcg aagctgctgt tcatgctgct
     4621 gatccattaa ctgtccaatc aataaattgt ggagagaggc aatggcctga tgactttggg
     4681 tgactgaaaa tccttcacga acaagtaatt ggatcatttg ctccaaatgt tctaatcgac
     4741 tcggaaaagc gggcacagta ttgatttcta attctgcccc acaagggaaa cgtgtataaa
     4801 ggtcatagta attttccata tactgcagca actgttcatt ccaaggcaaa gttggatctg
     4861 gaagcgctaa ttcttcttcc attgtttcag ccattttttg caagagctgt tgtttgtttt
     4921 tgaaatacca atataaagca ggtgcttgaa catgaattct gtttgcgatg tttcgcatgg
     4981 aaagctgcga aagagcttgt gtttcagcca ggatatcaaa ggcggcctga atgatgattg
     5041 cttgagaaag ctttatttcc attttatcaa cctcgtgtat tgcattatct ttttaaacat
     5101 ggtaaattgt tagtcggtct aattttacag tgttaaatta gtcctgtcaa tttaaacaaa
     5161 ggggtgtaat caacgaatga aattaatctt acgacatgcg aaaaaatata aagtagctgt
     5221 ttttatttcc ttgctgtccg tagcagtcat ggttgcagca gcattatggc aacctaagtt
     5281 actacagcaa gtattggaag ctattattac agaagataat gatgaaatga aaacaattgg
     5341 tatttacctg atcagtctgg ctttgctagg attagccgca ggtgtaacga acacgatttt
     5401 ctctgcaaaa gtagctcaag gcgtaagtgc agatatccga gaagaagcat ttcgaaagat
     5461 ccagacattt tcatttggaa atatcgaaca gttctctgct ggaaatcttg ttgttcgtct
     5521 aacaaatgat atcacgcaaa tccaaaattt agtgatgatt tctttgcaat cactttttcg
     5581 aattccattc ttatttattg gtgccttcat tttagctatg atcacgatgc cgcaattatg
     5641 gtggatcatc gtagcgctta tcgttacggt atttctgatt acggcacttt catttacaag
     5701 gatgggcaag cacttcatga tcattcaaaa actgattgat cgtgtgaata gcattgcaaa
     5761 agaaaactta ctagggattc gtgtagtgaa atcttttgta caagaagaaa accaattgaa
     5821 gtcattcagc aaagtaagtg aggacttaac aaaacacaac atcatagtcg gtacattatt
     5881 ttctgtgatg atcccgtcct tcatgctggc tgccaatttg gctgtggttg gggcgatctt
     5941 ttttgtgagc gatcttgtta aagatgatcc tacgttaatt ggtggaattg cttcattcat
     6001 gaactacttg atgcagatta tgatggcaat cattattggc ggaatgatga tgatgatgac
     6061 ttcaagagca gccgtatcgc tgaagcggat tgcggaaatt ttagatgctg aaccagatct
     6121 tacttattta gacgttccag aacaagagtt gacgggttct gtcacttttg accatgtttc
     6181 ttttcgttat cctggtgagg atacagatac gcttaaagat atcagctttt ctatcaaacc
     6241 tggagaaatg gttggtatcg tcggggcgac tggtgccggg aagtcaacac tagcacaatt
     6301 gatccctcgt ctatttgatc cgacagaagg caaagttgaa gtaggtggtg tagatcttcg
     6361 tcaagtaaat gaaaagagtt taagacaaac ggtttccttt gttttgcaaa aagcaatttt
     6421 attctcagga acgattgctc aaaacttgcg ccaagggaaa aagaatgcaa cagaaaaaga
     6481 gatggaacat gcttcttcta ttgctcaagc aaaagaattc atcgaaaagt tatctgatcg
     6541 ttacgaagca ccaatagaag aacgtagcag taacttttct ggtggtcaaa agcaacgttt
     6601 atccattact cgtggcgtta tcggtaatcc aaagatcttg atcctagatg acagtacgag
     6661 tgcactggat gctcgctctg aaaagctggt ccgagaagca ctcgatcgag atttgaaggg
     6721 aacaacgacg attgtcatcg cacaaaaaat tgcttctgtt gtcaaagcag accgcatctt
     6781 agtattggat gaaggcagat tagtcggcga aggaacccat gaggaattgg tagctggaaa
     6841 ccgtgtctat caggaaatct ttgaaactca aaaaggaacg gaggagtaag tatgactgat
     6901 ctaataaaag caagtaaatt tttctatcac tatttgaagc ggtacaaact ctcctttttc
     6961 tttatcttta tcacagtgat cattgctacc tatctacaag taaaggcacc tcaatacatc
     7021 ggggaagctt ttcaagaatt agctaattac gtaggaggct tgatgcaagg cgtagacgat
     7081 aaaagcaaat ttgtttctgt aatatggaaa ctcttgctgt tttatgtact agctagtgca
     7141 gccaacttta tctatagtat tttgttcaca caagtagtag ggaagtcaac gaaccgaatg
     7201 cgtatcggtc tattcaacaa attggaaaaa ttgacgatcc gtttctttga ttctcatcaa
     7261 gacggtgaaa ttttaagtcg ttttaccagt gacttagata atattcaaaa tagtttgaac
     7321 caagctttgc ttcaagtcat cacgaatgct gttttacttg tcgggatctt gatcatgatg
     7381 ttccgccaaa atgtagagtt agcttgggct acaattgctt ccactccagt agccgttttg
     7441 atagctgtct tgattatccg caaagcacgg aaatatgtgg atcttcagca agatgaagtg
     7501 gggaaactaa atggttatat ggatgaaaaa atcagcggac aacgtgtcat catcacaaat
     7561 ggcttacaag aagaaacgat tgaaggattc ttagagcata atcaaagtgt tcgcgaagca
     7621 accttcaaag gacaagctta ttctggtcta ctatttccga tgatgcaagg aatgtcactg
     7681 gtcaatacag cgatagttat ctttttcggc ggctggttag cattaaatgg tgatttggaa
     7741 cgttcggtgg cattaggctt agttgtgaca ttcgtccagt attcacaaca atattaccaa
     7801 ccactgatgc agatttcatc tggatacagc atgatccaat tagcgattac aggggcaaga
     7861 cgattgaatg aaatatttga tgaaaaagat gaagtcaaac cagaaaacgg taaagcattt
     7921 gatggtgttg aaaaaggtct tgcgttgcag cacgtagatt ttggttattc tccagaaaaa
     7981 ctagtcttga aagatgtctc gatcaatgtg gacaaaggag aaatggttgc tcttgttggt
     8041 ccaacaggat cggggaaaac gacgatcatg aatctgctaa accgttttta cgatgtcaat
     8101 gatggttcag tagtcatcga tggaatagat atccgagatt atgatcttga tgcattacgt
     8161 agacatgtag gaatcgtttt gcaagaatct gtattgttct ccggaacgat caaagacaat
     8221 atcacattcg gcaaaccgga cgcttcagat gaagaagtca tcaatgcagc aaaacaagcg
     8281 aatattcatg acttcatcat gacgttagaa aaaggttatg atacagagat atccgaagaa
     8341 aataacttat tcagtacagg tcaaaagcag ttgatcagta ttgctcgaac gatcatcaca
     8401 aatccatctt tattgatttt ggatgaagca acaagtaatg tagacacggt aacagaagca
     8461 aggatccaaa aagcaatgga tgaagcaatc aaaggaagaa ccagctttgt gattgctcac
     8521 cggctgaaaa caatcttgaa tgcagataga atcgttgtct tgcgtgatgg ggaagtcatt
     8581 gaagaaggca gtcatcatga gttattagca gaaaatggat tttatgcgga actttatcat
     8641 aatcaatttg tatttgaaag ttaaattaaa agaacctcgt tataacgggg ttctttttat
     8701 atattttatg ttgtttgttt ttataagggg ca
//
LOCUS       DACEDO010000093         8539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEDO010000093 DACEDO010000000
VERSION     DACEDO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.094"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(44..1510)
                     /locus_tag="IWO52_002531"
     CDS             complement(44..1510)
                     /locus_tag="IWO52_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0356764.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1897..2493
                     /locus_tag="IWO52_002532"
     CDS             1897..2493
                     /locus_tag="IWO52_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0356765.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2551..3033)
                     /locus_tag="IWO52_002533"
     CDS             complement(2551..3033)
                     /locus_tag="IWO52_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356766.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3139..3336)
                     /locus_tag="IWO52_002534"
     CDS             complement(3139..3336)
                     /locus_tag="IWO52_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356767.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3631..4389
                     /locus_tag="IWO52_002535"
     CDS             3631..4389
                     /locus_tag="IWO52_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0356768.1"
                     /translation="MKKKTFKKLLFLGGTTALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4449..4877
                     /locus_tag="IWO52_002536"
     CDS             4449..4877
                     /locus_tag="IWO52_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356769.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4923..4995
                     /locus_tag="IWO52_002537"
     tRNA            4923..4995
                     /locus_tag="IWO52_002537"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4956..4958,aa:Gln,seq:ctg)
     gene            complement(5145..5516)
                     /locus_tag="IWO52_002538"
     CDS             complement(5145..5516)
                     /locus_tag="IWO52_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0356770.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5731..5916
                     /locus_tag="IWO52_002539"
     CDS             5731..5916
                     /locus_tag="IWO52_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356771.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(5999..6187)
                     /locus_tag="IWO52_002540"
     CDS             complement(5999..6187)
                     /locus_tag="IWO52_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356772.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6211..6474)
                     /locus_tag="IWO52_002541"
     CDS             complement(6211..6474)
                     /locus_tag="IWO52_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356773.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6632..6889
                     /locus_tag="IWO52_002542"
     CDS             6632..6889
                     /locus_tag="IWO52_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0356774.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6960..7151)
                     /locus_tag="IWO52_002543"
     CDS             complement(6960..7151)
                     /locus_tag="IWO52_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356775.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7292..7612)
                     /locus_tag="IWO52_002544"
     CDS             complement(7292..7612)
                     /locus_tag="IWO52_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356776.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7635..8069)
                     /locus_tag="IWO52_002545"
     CDS             complement(7635..8069)
                     /locus_tag="IWO52_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0356777.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8404..>8539
                     /locus_tag="IWO52_002546"
     CDS             8404..>8539
                     /locus_tag="IWO52_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0356778.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     K"
BASE COUNT         2704 a         1684 c         1353 g         2798 t
ORIGIN      
        1 ggaactgctg caaataagca attcctgttt ttatgttcat tgattaatga atagagccca
       61 ttagtttttt gattggttct ttcacagtaa atagcaagat accaccgacg attgctactg
      121 caccgataat accgaagtaa gcagattctg ttcccggtgt gtagaagcgt gcaatttgtg
      181 cattgattgc ttgtgaagaa gcatcagcta agaaccaaat agcgattgtt tgtgattcaa
      241 atgctcgtgg tgctaatttt gttgtcacag acaaaccaac tggtgaaaga cacaattcag
      301 caataaccac gatgaagaaa cttcccaaca accacaatgg acttactttt ccacttgtac
      361 cataaagcat acccggcaac atcaatagca agtacgataa tcctgcaaat aaaagtccaa
      421 gagagaattt cactactgta gatggttgac gttcaccaag tttcgtccac atcgttacga
      481 atactggtgt cagaataaag atgaaaattg gattcaatga ttgataccag cttgcttgga
      541 tcgttacacc aaatagcgta gattgtgtac gttcactagc aaataaagct aagatagatg
      601 aaccttgttc ttgtaatgac cagaaaatga ttgctgcaag gaataatggg atataagccc
      661 atactcttgg tttttcttca gcagttacat cttttgaagt cagcattttg ataaagtaat
      721 acaacggtaa gataatcccc aatacactga ttgtattgac aaaaaagtca attgtcagat
      781 gaccagttag ttgtgccccg ccgaatactg cagcgataac aacaagtgcg atgatcaagt
      841 tacggatgaa tcctttacgt tcttcagttg tcaaaggatt cggcggtgtt gtacccactc
      901 cgtctaatgt cttgcgtcct tggatgatgt attgtaataa tccaaagaac atcccaatag
      961 cagctaaaga gaaccctaaa tgatagttat attcttgacc gattgtacca acgattagtg
     1021 gtgctaataa tgcccctaag ttgacaccca tgtaaaaaat cgaaaatcct gcatctcttc
     1081 gcaagtcagt ctttgaataa agatgtccga ccatgccaga aacatttggt ttcaaaaagg
     1141 cggttccgat aataatcaat gccatcgaaa caaacaatgt acttacacca aatggtaaag
     1201 ccaagacgat gtggccaagc ataatgaaaa ctccaccgaa gaatacagtg cgttttgctc
     1261 ctaacactcg gtcagatacc catccgccga cgatactaga catgtagatc aaagatccgt
     1321 agatcgacat gatagctaat gctgttgctc gtggtaatcc aagtcccccg ttggcaactg
     1381 tatcgtacat ataatagata agtaatgcac gcataccgta ataactaaat cgttcccaca
     1441 tctcagtgaa gaacaaagtt gacaatccct tcggctgccc gaagaagccc ttgtcttgct
     1501 gtttttccat aatctatacc ccaattcttt aaattttcag aaaatattat acatctttac
     1561 cgaaaaattt aactaaaaaa cttatttaag aaatataaac taattttcta taaaagcaag
     1621 taatttttaa tggcaatatc ataaaaaaaa gaagatagag atttaataat tatatatcag
     1681 ctgttttttt caaataagca aaaattccta aaacacaaca atgaaagcga taactaagaa
     1741 aatccttaca taacgatttt tcttattaaa attgtttttt gaacttcgct tattttttgt
     1801 gagcatatct gtttacttac tttttgtaag gtgctatact tcatttcgta atcaattaaa
     1861 aaaatgcaaa acgcaaatca aaggagaaat tttattatga caacttttac tgacactttg
     1921 aaaaaccgcc gttcaatcta tcatctagga cgtaatgttt cactttctaa tgaagaatta
     1981 acaactttga tcaaagaagc aattaaagaa agcccaactg ctttcaatgc tcaatcaaca
     2041 cgcgcagtta tccttttcgg agatgcacat gaaaaacttt gggaaatcac tgaagaagca
     2101 cttcgcccat tgactccagc agaagctttc ccaaatacac aaaacaaatt agctggtttc
     2161 aaaaatggtt atggtacagt attattcttc aaagatactg acgtggtaaa aggtctacaa
     2221 gaacaatttg aattatatgc agacaacttc ccagattggt cagaacaatc aaacggaatc
     2281 gcaacagcta atacttgggt agcattagtt gataaaggat tgggcgcaaa cttgcaacat
     2341 tacaacccag ttattgacga agcagttgca aaagaatgga atatcccttc aaactggaaa
     2401 ctacgttcgc aattagtatt cggttcacca gaaacaccgg ctggggaaaa agaatacatg
     2461 aacgatgccg atcgcttccg cgtatttgga taattttatt tgaaataaaa aacagaggtt
     2521 gtgacaaaca tcttgtcgca acctctgttt ttattctgga tcaatcataa tatcaatctc
     2581 tgcctcatcg tttaagcgtt gggacaaaaa tggaactaat tcttctaatg atgcagaaaa
     2641 tttcagcatc ggtcctcgcc atccgtttcg gaaaaaattc atttttacga ttttagaacc
     2701 ttttggctga tataatacaa ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa
     2761 gctattcaaa acaagtcctt cttctgtgat tcctctactg tccaacaaaa aactaataaa
     2821 aatcattgct gacaagatcc cttttgccaa ttcatctgct gtaggatgac tgatgagtgt
     2881 ataccaaatg actagtgaca ctaagaaggg tatcaccaag tagacccatc gatttcgcac
     2941 agtccgataa ataatttctt ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa
     3001 taaaaacaat agaaccgtaa agaatgttac catgacaact cgcctcgctt tttcttctcc
     3061 agaaaatagg aaaagaatac tgatttacct tctcaaagaa attggacttg cagcacgccg
     3121 cacgtccaat tgttttcttt attcaaatgt atcatatttt gcaacaacca tgcttgcttt
     3181 ttcatctaat aattcttggt cctctgcagc acattgggca actttgacca cggcgcagtt
     3241 tgtcattttt gatacaactt caccaatgac cgtttttgta aaaccaattg gtttgcatgc
     3301 atattgttct cctaaattaa tcatttcttc cgtcatcaaa atcctcctaa actgctggaa
     3361 cttttctcca cattaccact gctatttctt tggagattta actcattgat ttttatttac
     3421 agttaaaaat caaccttaca ggcattatag cgtactgttt tcgttttcac aattaaaaca
     3481 gctgttgtat ttagaagaat ttttttcaaa aaattaaaac atattcctta aaaactaatt
     3541 atttcctaca tattaaaata taatacttcc atttccaata aaaatacatt tataatagga
     3601 aagataaaca gctgaaaagg gagatttcaa atgaagaaaa aaacgttcaa aaaactattg
     3661 tttctagggg gcacaactgc tcttttatta agcggtacaa gttattacct acatatccat
     3721 tctccagaac atacaatgaa cactgcacaa aaaagttcaa cacaaaaaac gaaaaagaca
     3781 aaagagaaag cagaagtttt actagatgtt ccattagaga gccagtttga ttctccttct
     3841 ttagaaaatg gctgtgaagt cacttcgctt tctatgcttt tatcttttta tggttatgaa
     3901 acaactaaaa accagttagc tgagcaattg gattacgtcc ctgttttcaa tgcagatggg
     3961 actcatggcg atcctaatga aggatttgtt ggtgatatca gtggcggaga ctgggctatg
     4021 gtcgtctatg ttcccccagt tgcctccctt gctcagcaaa tcgtccaaac ggattatcac
     4081 acttttccaa aaaccgatgc tcaactcgat gatatcaaaa aagcactatc taaaggaaat
     4141 cctgtctgga cttctgtgac tatcaatttt gaagtcccag atgaaactga ttttatgact
     4201 tggaccacaa ataatggaga agtaagagtc actcctcttg ttcatgcttg tgtagtcact
     4261 ggatatgaca aagaaaatat ttatgtcaac gatccctatg gtgtaaaaaa tcgagcagta
     4321 ccgatcaacg atttttcggc gattttcact gcaatgggtg gacagatgct aactttggag
     4381 aaaagctagt aattcaaaca aaaatccctt atactcaaga taccaaacct ttgaagggag
     4441 cgtagataat ggaaaaattc acacataaaa aaatggatcc aaatgaaatt ccgattattt
     4501 ttgtccgtga ctgcaaggga aatgttcaag gtaaagtctc tattaatgag tggaacgagc
     4561 gtcgcagacc tgctaccctc aatgaactgg aaatcaaatt gtatcgtcaa tctcttgttt
     4621 attacgcaga tcaagagtac gagaaagcca ccgatttatt gaaatttcta attgctcgaa
     4681 cagagtatac tcgctttgaa tatattgaac gtttagcaaa catctatcat attatgaatg
     4741 aacctgtcaa agaatatcag ttattggaca ccgtcttgtc agtagcagaa ctgattgccc
     4801 ttcctgctgg attagaaaag aaactagtac gccgcctttt acgagtaaag caacaactat
     4861 ccgatcaaga aaaataactt gcgttttttt aggaaagtgc tatgattatt ttgttgaata
     4921 aatgggatgt agccaaactg gtaaggcact tgactctgaa tcaagcaatt gtaggttcga
     4981 accctgccat cccaatttga tgtgcttgct gaaaaaatga accccctgta aaagaacaat
     5041 ctttttgcag ggggattatt gtattatttt tactacttgt atttagttta cataattaaa
     5101 ttatgaataa taaaatcggg cgtattctta atacaaaaac tgatttaccc aataattgct
     5161 aatgctttct ccggaacttt ttctctagga acttcctcat acgtttctat gtgtgctcct
     5221 ttattgctaa ttttcaaata atgattttct tttaacacag aaagaccatt aaattcaacc
     5281 gttcgttctt tcccattggc atcagcagct ttctgcacat accttgctgt accatattca
     5341 tttacttcct ctggtttttg tgtctttaca tagacctctc ctttttcaac taatggattt
     5401 agctgatcaa gtataccagg gatttctcca taacttcctt cagtataata tttcagtcca
     5461 aatagcaaaa taccactaat cgccgccagt ccaatcaaat atttaatcac tttcatcttt
     5521 ctacctcctt gttatatatt cagtatatgg gtcagttaaa gaaattccat ccgtcccaag
     5581 ttggagattt ttctcctact ttagaagcga gaatcgagtg tttttttagt aaaaatgatc
     5641 gttatttgat attattgacg tgtggataaa tcagtttttc tcgttttgtc catgtaaaaa
     5701 atcatgttga tagcaaagga tgtgttcaaa atgaatcaag aagaattatt tcaaaaagtc
     5761 aaggaaatga ttaagaacgg caatttcgat ggcgcaaaac gatttatcga agaacataaa
     5821 gaacaattag gaccttataa agagaaagcc caaaatcttt taaaagatgt aaatatcgac
     5881 agtgtcaaaa acaaatttaa aaatctattc aaataagtaa aactttcact tttgtaaaat
     5941 caaaggagga caatgctgct gcatcgtcct cctttggttc ttttctgaat acttcatact
     6001 aaaataaacg tgtcggatcg taatgataag tcacattgcc gttcagtgag gcttttccca
     6061 ccacatattt acgattctcc aacttcagcc acgcttctcg aaaagccatt aattcttctc
     6121 tttttacatc aatcttttcg atttttccat cgatcaaatc ttgaataagt tgttcatatg
     6181 gattcatttt tttcctccat ctgctgtcaa tcaataagtt gtaaaaatag gttcatatgc
     6241 ttttttggta tctagatctt ctctgacttt gactgtcgta taaataattt tttctactgg
     6301 gatactataa taacttcctt caccattatt gaatcggtga acgtctttct ttgctaaaat
     6361 accatttatt ttattcgtca gtacacgttg ttcgacactg ttacttacaa catagtaaat
     6421 cacccgaata ttattgatca aatgcaaatc gatttctaac actacatgat ccatgaaaat
     6481 cccactcctt ctttcacttt gattatagca agtttctcct ctaatttcaa aatcgataca
     6541 gattttaccg gataaatcca caaaactgct tgcgccattt cgcacaaagt gttattttgt
     6601 atcatggact ttatttatcg gaggcaatag tatgatcgcg atcggtcaat ttgtttttta
     6661 tattcctttt tttattatga taagcattct tttctattac ataaaatgga caaaaaagaa
     6721 gttttcagtt ttactggcct ctttacctgc tgtatatttt acttatcaaa ttttttcttt
     6781 taggcattgg gaaacaacgt ctgtattaat aacacacatc atcgaattga cgctttcagt
     6841 aatttttctt attatttgga tctatttttt atataagaat cagaattgaa tccctcgcat
     6901 gtttaaaaag aaaacgatta aagatgcagc aaatttctgc cgatctttaa tcgttttttc
     6961 tattctgtta taccaaaaaa atgtttgaat cggtgaagga tcgttccttg ttttaattct
     7021 ataaattctt gtgcgatcgg gtacttttct tccaaatttt ctttgttctt ttcgatcagg
     7081 cttttcgctt cttcatattt tttatctgca catagtgctt ttacttcctg taatactttt
     7141 cctgcttcca tttgcctcac cctcttgcca ttttattgct ttaagtttta tttaagttat
     7201 acgcattatt ctagtttcat cccaaataac cttttttcat ttttactcct cgaactaatc
     7261 agttttctaa aggctgattg gttcgtcttt tttatttttc ctccatttca ttctgctctt
     7321 ctgttaattt atctaatgct ttgattgatg ctgcggctct tgcataatca tagctgtata
     7381 attccataaa tttatcaggc agaacaaacc acgctggatg ataaccgtta gctgtgacag
     7441 ggaatccttt atcttgttgg ttatctccat cccatctcag agcaacacct ttttttcctt
     7501 tccactttac taaagcgatc gagtatccct tctttgtttg tgtgccgtct tcaataattg
     7561 ctaaaatttc tattttttct tttggcgcac ggacttcttg aggacgaacc attttctatt
     7621 cctcccttat ctcattactt cacgaccaac acactgcaag gggcatgtga tacgacataa
     7681 gaagcggtcg atccaactaa tgcttgttgt atagcacctt ttcctgtcgc tcccataacg
     7741 atcaaatcaa tattttcttg tccagggatg acattggcaa tcaaatttct tggatctcca
     7801 gtttcaacta ttgcgtggat ctcagcaatg ccgtactctt tggcatcatt gatttttttc
     7861 agcatggcta cttctgttct ttgtttttca tctgcataga tttttgaaaa ggcataggca
     7921 ctatgggtca gctcaacttt gttgatgatt gacacgataa ataagtgggc ttcattgtcc
     7981 tttgctaagt caagggcttc agcgaacgct ttgatcgatt gtcttgatcc gtcaactgct
     8041 accatgatgt tctggtatct tttactcatt ggaaccgcct cctttttctt ttattgtaac
     8101 atctcaagca aaaatacaaa aaatatacgc aatatgtaaa taaaatgatg aacaaaagca
     8161 atttctttca agttatttat acatttcttt tctcatcaga acacctgttc taatcccatt
     8221 atttctgcat tatgtgaaat tcctcatcat ttttcacatc gagcgaacat cattctgtta
     8281 caaaaaacgt ggatttgtca taaattgtca tgcttctttt acctatgaaa tattttcacg
     8341 tgttaaagta ttcctcgttg aacacaacaa ataaaacttt ttaacgaata aaggagattt
     8401 ttcatgacat cacttaaaac tttacttttt ggaacaactt tggctgccgg cgctgcattc
     8461 ttcatgggaa cgactgctca tgcggacgaa gcttataccg ttcaatcagg tgatactcta
     8521 tcaacgattt ctcaaaaat
//
LOCUS       DACEDO010000094         8492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEDO010000094 DACEDO010000000
VERSION     DACEDO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.095"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWO52_002547"
     CDS             complement(<1..173)
                     /locus_tag="IWO52_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0356779.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWO52_002548"
     CDS             complement(593..1714)
                     /locus_tag="IWO52_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ0356780.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IWO52_002549"
     CDS             2042..3451
                     /locus_tag="IWO52_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0356781.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWO52_002550"
     CDS             3448..4176
                     /locus_tag="IWO52_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0356782.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWO52_002551"
     CDS             complement(4304..5215)
                     /locus_tag="IWO52_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0356783.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWO52_002552"
     CDS             complement(5232..6239)
                     /locus_tag="IWO52_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0356784.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWO52_002553"
     CDS             complement(6236..8365)
                     /locus_tag="IWO52_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0356785.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWO52_002554"
     CDS             complement(8370..8477)
                     /locus_tag="IWO52_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356786.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tt
//
LOCUS       DACEDO010000095         8428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEDO010000095 DACEDO010000000
VERSION     DACEDO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.096"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(71..1024)
                     /gene="menA"
                     /locus_tag="IWO52_002555"
     CDS             complement(71..1024)
                     /gene="menA"
                     /locus_tag="IWO52_002555"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0356787.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1037..2140)
                     /locus_tag="IWO52_002556"
     CDS             complement(1037..2140)
                     /locus_tag="IWO52_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0356788.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2230..3165)
                     /locus_tag="IWO52_002557"
     CDS             complement(2230..3165)
                     /locus_tag="IWO52_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0356789.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3467..5401
                     /locus_tag="IWO52_002558"
     CDS             3467..5401
                     /locus_tag="IWO52_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0356790.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5858..6274
                     /locus_tag="IWO52_002559"
     CDS             5858..6274
                     /locus_tag="IWO52_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0356791.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6288..6854
                     /locus_tag="IWO52_002560"
     CDS             6288..6854
                     /locus_tag="IWO52_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0356792.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6877..7863
                     /locus_tag="IWO52_002561"
     CDS             6877..7863
                     /locus_tag="IWO52_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0356793.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8018..>8428)
                     /gene="scm"
                     /locus_tag="IWO52_002562"
     CDS             complement(8018..>8428)
                     /gene="scm"
                     /locus_tag="IWO52_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0356794.1"
                     /translation="TSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTS
                     DSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALP
                     KTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2482 a         1622 c         1709 g         2615 t
ORIGIN      
        1 aggagctatt tttatttggc tgttcttatt acttgatgca gggcagtttt ttcacaacct
       61 cttcatgctg ttaaatggct tgcataatga ttgttagtaa tagccccaat acataactgg
      121 cattgaataa gaccatattt ttgatggaat agctgaaact gataggctgg ggtaacgttt
      181 gcttgaattg atccaaatgc ttcttcacaa ttggcaaggt caagaaggtg accaagatcg
      241 gccattcata gataccgctc aacaacccca ttaaaatcac gaaatagcaa gcatacatca
      301 atagctggaa taagactgtt cccgcaggac gtccgatata aaaaactagt gtatagcgat
      361 gattttcaat atcacggtca agatcccgca aattattagc caacatgata ttggcaatcg
      421 taaacaccaa gggtaaggaa gctaatacga ttgctgctat agcccaaaga tttcctacca
      481 aagcaaattg tccagatgaa aagttgactg tcagatcata gacatctgaa ccgaatgtat
      541 tgacataaac gaccatggca aaaattccaa ggcccatagt caccccactg aaaatttccc
      601 ctaaaggcat tcgagataaa ggaatcggtc cgaatgtata gaatactcca ataaaacaac
      661 agataaatcc catcaaaagt aaaagccaac ccgtattgat cgttagaata acgccgataa
      721 caaaaacaaa tgcaatcata gctaaaatca tgtttcgaat caatacagga gatagatttc
      781 tgcggccaat gacattttct tcatacttat atacttcaga atgagccttt tcataatcca
      841 tgtaattatt gatagcggta gtcgctaaat caaaaactag cattccaata aaaaataaaa
      901 aagtgtttag ccaatgtatc tcatgaaaaa aagcgataga gaataatacc cctaccgcaa
      961 aaggaaatac actagccacc tttgttcgta tttcaactac ttctaaaaag atttttagtg
     1021 tcatggttcg tactccttat tttagttcag aacggtcacc catcgtgtaa tgggagtcct
     1081 ccgataattt gaaggtgtct ttgattggat cggtcacata tactttatcg tcttttgtca
     1141 cgaagatcgc ttccgtcccg ttattcaatt cgctttcgac gtattccagt ccttttttca
     1201 cacccattga aaagactgct gtagacaacc cgtcaccatc aattgattta tctgttatga
     1261 tcgtgacact tgcgatatca ttgtcgaacg gataacccgt ttttggatca aacaaatgat
     1321 ggtatttttt gccatctact tctaaataac gctcgtaaat tccagaggta actaatgttt
     1381 tgttccgttc tttaatagaa cctaaaacag atccgcgagc catgttcgga tcttggatgc
     1441 cgactgtcca gtcttggttt tcgcctcttg ggctgtggcc taagacatag acattaccgc
     1501 ccaagtcaac gatggcagtg gtcactcctt gttttttcaa gactttcact acttcgtccg
     1561 taatgtaacc tttggcgatt gcacctaaat cgatgatcat tcctttttct tttagataaa
     1621 cagttttttc tttgtcattt agttcgatct tatgataatc cactaatttc aatgcctgat
     1681 cgatttcttc ttgggaaggt tttctggcgt catcaaaacc gattcgccat aattgtgtga
     1741 ttgcaccaat cgccatatcg aatccgcctt gagaatcttg gctatattcg taggcccgct
     1801 tgactaacgt gtagacgtca tcagaaactt ttactggttt gatcccagct tgctcattca
     1861 cttcatcgat ctcagaacct ttttgattga tcgtgatttt atcaccaagt tcttttactc
     1921 gatcgaaggc aggtttcaat gctgattctt tgccttcatc atacacacgg atacgaacgt
     1981 aagtccctaa caagaactgt tcatcagaat atggatcttt caatatcttg gtagctgctg
     2041 aactttcagt ggttgtttta tttgctgagt tgctgcagcc agttagcacc ataaaagaga
     2101 tgcccagtgt gattaaggaa aatagtagtt ttttccgcat ctttttcctc tcttcatttt
     2161 gaaaggtgag acgactgcct aatctggcta tcgtcccacc ctccaacttt tattagtatg
     2221 attatgtgat tatttttctg ttacgatatt atctacttcg attgtttgag tgtcgccttt
     2281 ttcagctgca ttcactaatt gttgtgcgta catgatgaat gaatgtgagc tgtgtgtagc
     2341 acctgtaaca acttctacat tagcaggtga gccatcttct tcgcccattg ctttgacgaa
     2401 ttcgtcgttc agagtaggga tgtaagtttt tggttctgta ccgctttttg ctttcatatt
     2461 ttcattatat tctgtatcat cttgttttga tttaccgtct ttgtcgatat aatcaaagtt
     2521 tgattcagtt actttgccgc cagcaactgt catttcgaat tgcacacggt agccgttaga
     2581 gtagttcttt tcttcaagct tgtaagtgcc gtctttcaaa tcagcaccat tgtcgatttc
     2641 gattgtatct gtgttaccag cttgagcagc ttggatcaat tgttgtgcgt agttttggaa
     2701 gctttcagat gagtgagtag ctccagttac gacttccaca ccgcttgcgc tttgtgcttt
     2761 taagaattgg tcattcaact caggaatgta tttttctggg tttgtgccag attttgcttc
     2821 catgtttttg ttgtattctg catcttttgt ttttgattcg ccgttttcat tgacgttatc
     2881 atattttgat tcagtgattt tgccgtcttt tacaaccatt tcaaagactg cacggtaacc
     2941 atttgaataa tttttttctt ctaatttata tgtgccgtct tgcaaatctc cgccagctac
     3001 aacttttgca gatgattctg ttgtgctttt tgcagtcgaa gattgtgctg tagatgattc
     3061 agaagaagag tttgagcttc cgttgtcatc tgcgccgcaa gcgcctaaca ctagagctga
     3121 aaatgctaat acggcaaatc cagttacaaa acgttttttc ttcattttaa taccccacca
     3181 ttccatttct agtccacaat ttttctttgt gattcattat acattctaat ctttttagcc
     3241 tataagtcaa ctatttttga taaataaccc atgtaatatt tcaaacgttt tctttcagca
     3301 aatttcgtga aaaaacgctt tattttaaaa tttgaaaaaa atccaaaagg agttagacaa
     3361 aagaacagaa catctatata atatatggag attgtgtaat aagtatcaaa ctaaaagaat
     3421 tatcatatat tcattatata gatagtctat aaggagcgga tgatcaatgg caaagcaaaa
     3481 tattgtcgtt gtaggagcag gatatgccgg tgtttcagct acaaaatact tggcaaagaa
     3541 actgaaaaaa gaagatgtag caattactct gatcgatcgt cattcgtacc atactatgat
     3601 gaccgagttg catgaagtag ctggcggacg tgtagaacca gaagcaatcc aatacgatct
     3661 gcagcatttg tttgctcgtc aaaagaatgt acaacttgtc acagacactg taatcggtat
     3721 cgataaagaa cgaaaaatcg tcaagaccaa attaggttcc tatccttttg atcagctgat
     3781 ccttggaatg ggcggagagc cgaatgactt tggtacacct ggtgtcaaag cacacggatt
     3841 caccctttgg tcatttgaag attctgtaag gattcgggaa catattgaga agacagtagc
     3901 aaaagctgca ttggaaccag atgatgaact gcgtaaagcc atgctgactt tcgttgtctg
     3961 tggttctgga tttaccggta ttgaaatgat tggggaactg atcgactgga aaaatcgcct
     4021 agccaaagat tataaattag atccaaacga aatcacactg atggtcgtag aggcaatgcc
     4081 gacgatcttg aatatgttgg atcgaaatga tgctgcaaaa gctgaacgct atttgcgtaa
     4141 gcacaacgtg gaattattat tgaatgcacc aatcgtagaa gtggctgctg atcatatcaa
     4201 attgaaagac ggacgcaaca ttccgactca cacactgatt tggacagctg gtgtcaaagg
     4261 aacttctgac gctgctgact ttggattaga atcagcacgc ggacagcggt tgatcgccaa
     4321 tgaatatatg caggcaaaag gctacgaaga taaaaacatc tatatcattg gtgatttagt
     4381 cttttatgaa gaattcccag gaacaccgac accgcagatc gttcaagctg ctgaacaaac
     4441 aggacacaca gccgcagcga atatcgtggc ttcgatcaaa ggcggagaaa agcataaatt
     4501 taaaggaaac tatcaaggat ttatggtttc tattggtgcc aaatgggggg tagctaacct
     4561 atttgataag atccatttaa gcggattttt agctatcgta atgaaacata tcgtgaattt
     4621 gaaatacttc tttgatattc gttctggtta ttatatgttc caatatttaa tgcatgagtt
     4681 tttccatatc aaagatgatc gcaacgtgac tcgaggacat tcttctcgtt acggcaatgt
     4741 cttatggagt gtgccacttc gtattttcta tggtttagtt tggttcgttg aagcttccaa
     4801 aaaaatcatt gggaatggtg aagtgttcaa accaagtact tggtttggtg aaggatcatg
     4861 gttcactgga aacagtgtgt tcccttggac atgggaatac aatgtaacag atgttgccag
     4921 tggtgcttct gctgctggtg atgcaaccac agctgccagc ggagcagggg caagtgccgg
     4981 agaagcagcg actcaagctg cacattttgg tttgagttat gcatacggtg aagaaccaat
     5041 ggctgtgatt gagaagtcac cagactggtt cacaaagatc atgcaagtga tcatgccaaa
     5101 caatgatgta gcaatgttct tccaaaaatt catggtattc tttgaaattg ccttggcatt
     5161 agcattgatt gctggattat tcacatggct atgcagtgcg acaacgattg tattggtgat
     5221 cacgttctgc ttatcaggca tgttttactg ggtaaacatt tggttcattt ttgttgcttt
     5281 cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa ttccatggct
     5341 acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg gcaagaaata
     5401 aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg agacaaaccg
     5461 tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa gcctccatga
     5521 gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt gttccagaag
     5581 tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat tttcgtaaat
     5641 tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata aacggtgttc
     5701 cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag ctgttttcta
     5761 ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt ttttatgcta
     5821 aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag aatttgctga
     5881 aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg ttctttcttt
     5941 tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa aagaagcggt
     6001 actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag aagagagcaa
     6061 cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag atggcgatcg
     6121 agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag gctgggcaac
     6181 gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag tccgttcaac
     6241 agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg agtaaattac
     6301 aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt ttattggaaa
     6361 atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta gccaatctaa
     6421 tcacgatcgt tgccattttt accatgaaaa aacgagacag cttcttacta ctttggctac
     6481 gactattcct gactactttg ctaggcggaa cgatttccac attcctttat agtatgagtg
     6541 gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca aaacgtgtca
     6601 gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag cttttgatgg
     6661 cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt tcagttttag
     6721 ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga catgtgcaga
     6781 cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg agcccaatgt
     6841 ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc aatgtgggaa
     6901 aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga agaaactgtc
     6961 cgcctgaaaa acaaacctgt cgaacaagca atcaaagaaa cgatccatgc cggcggaaag
     7021 ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca agatcgagac
     7081 aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact gatccatgat
     7141 gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag tcagtttggt
     7201 aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa actattgtct
     7261 gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga aaaagtattg
     7321 aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt aaaagaatat
     7381 ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc tgttggcgct
     7441 tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg actttctatc
     7501 gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag ccaggaaatc
     7561 ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt gatctactct
     7621 ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc aatgacagaa
     7681 gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga agaagctagg
     7741 aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt acctgccacc
     7801 tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga tcgcaaagac
     7861 tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta cgtgtttttt
     7921 gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag agcaacgtcc
     7981 gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat ttcttataac
     8041 tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt gttatccaat
     8101 tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca ttgtcattgc
     8161 ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt gagaaggaga
     8221 cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg cttgaagttg
     8281 atgaatctga gctatcactt gtggtagagg atgtatctgt actgtcactt gaagctgacg
     8341 tgtccgtgct atcacttgta gtggtagatg aatctgagct atcacttgtg gtagaggtag
     8401 tatctgtact gtcacttgaa gctgatgt
//
LOCUS       DACEDO010000096         7975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEDO010000096 DACEDO010000000
VERSION     DACEDO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.098"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(2..1846)
                     /locus_tag="IWO52_002563"
     CDS             complement(2..1846)
                     /locus_tag="IWO52_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356795.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            2058..2576
                     /locus_tag="IWO52_002564"
     CDS             2058..2576
                     /locus_tag="IWO52_002564"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0356796.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            2704..3387
                     /locus_tag="IWO52_002565"
     CDS             2704..3387
                     /locus_tag="IWO52_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0356797.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            3696..3769
                     /locus_tag="IWO52_002566"
     tRNA            3696..3769
                     /locus_tag="IWO52_002566"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3730..3732,aa:Arg,seq:tct)
     gene            3928..4287
                     /locus_tag="IWO52_002567"
     CDS             3928..4287
                     /locus_tag="IWO52_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0356798.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            4410..6041
                     /locus_tag="IWO52_002568"
     CDS             4410..6041
                     /locus_tag="IWO52_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0356799.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6103..6744)
                     /locus_tag="IWO52_002569"
     CDS             complement(6103..6744)
                     /locus_tag="IWO52_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0356800.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7026..7334
                     /gene="rplU"
                     /locus_tag="IWO52_002570"
     CDS             7026..7334
                     /gene="rplU"
                     /locus_tag="IWO52_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0356801.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            7361..7705
                     /locus_tag="IWO52_002571"
     CDS             7361..7705
                     /locus_tag="IWO52_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0356802.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            7718..>7975
                     /gene="rpmA"
                     /locus_tag="IWO52_002572"
     CDS             7718..>7975
                     /gene="rpmA"
                     /locus_tag="IWO52_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0356803.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKK"
BASE COUNT         2529 a         1320 c         1638 g         2488 t
ORIGIN      
        1 tttatacagg attcgggtaa tttcgagaga gttgaacagt gatccgatca atgaaagtat
       61 ctttttcctg cgttagtgtt cctgtgtaac gatccgctac gtctttcatc atcattaagt
      121 caaaacgttc ctgatcatct aattcttctg tgatgcctcg actaaatagg tttgtatcgt
      181 aatgtgcact atgttcgcaa caaatgacga aatgttcctc ttgttcgtgt atcgacaaat
      241 aaatttttcg atacagtgga ttttcgatgg catgagtttc tctaaaggaa tgctctaaag
      301 cgtggatcaa aagttgagcc agatcgtcat cgttgatagg aagttcttcg ggaagatctg
      361 cttggaaggt tatctgaata ttccgtttgg cagctaactt ctggtaacgc gctaagataa
      421 gatttgtcac ctggtggttg caaaaagaac acagcatttc gaaatttttt gcttcttcta
      481 atatcttttc cacatagttt ttagctttcg tttggtccgt atgcattaaa tcttgcagat
      541 tctccatctc ttggacaaat tccaggcgaa gtgtatttac ttcttggata tgctgtcgaa
      601 gctgctcata atgttgctcc aacaaatcat ttttgatttt taagaaatct acttgtctcc
      661 gatctttgtc ggtatttaag atgtgatcga ttagattgta gctgataata acgattagaa
      721 tgataatata taaaatcgta gaccaattga tcgttgtttg atagatacct aatgccgttt
      781 ggatgtaaat caagcaatgg ataatggcta ccaagacgat ccaaggtgta caagctacat
      841 aaaaacggtt attcttatat gcttcgccaa tgcatgcaag tgaagtgact aaagtgctta
      901 taacactagc aatattagca atcggcatta attcaggaag ttctgcagga gtaaaagttg
      961 cataaagcag aacgccgata aaaatcaaaa gttggaacgt acaaaaaatt ccgtaccatt
     1021 ttttatagta aaacattcgg ctccaatagt agcagatgat gaaagctgac gttaagataa
     1081 taaataaatg agataaaaaa gaatgaacga atggttcaaa aagaatacca cttaaaatat
     1141 cttctgatac agcttcaaat cctaatgcca aagcaaagca acttaagaga ataagtgagt
     1201 attcaagacg tttttgttta tacatcaagt agatcgtata gaaaaaggtc cctatagcga
     1261 taaaaacagc gattatcatc gtcaatactt gcggcactga ttgtgaataa ataaaactaa
     1321 tcaatggtcc agttgttcct agataaactt tcggtggcag tccagcatag ttctgataag
     1381 gagatgagac tgtaagggtc aatttttttc cgatataatt tgctggtaaa tctatcacag
     1441 ccaatgagag tccgggattg ctttcttcct gataatcttt ccgttggtat aaaatctttt
     1501 cgcctagata tacagtaatc cattgatggt ttcctttgat tagtaaattt gcttcgtcca
     1561 acggtttttc aagtgtcgtt tccataatga atgtttcgtc tttatccacg gtaggaatcg
     1621 tacgagtgta cttgctgtca aacttaaaat caggatcatc ttttgtatag aaaacccacg
     1681 aatcttttat ttctttcatt tgattgctgt ccgcaggaga aaaaataaaa taaaagaaat
     1741 aagcaattcc tgttaaaaat aacaatgata atataatgat tatgctccac tttatacgta
     1801 aataaaaaga ttctttttta ttatatatgt tattaaaaaa agacataaag gtattccttt
     1861 ctaaaacttg taatgttagg gtaaacgttt tttaaactaa cgtcaattat ttataataaa
     1921 aaatgagata aagatgagga aatagaatta gaaacatata attattaaag ggaaacattt
     1981 ttacagctat aatttgaaaa aaatttatcg ttattatatg attaagaagg attgaataaa
     2041 gaaagaagtt gaaaacgata cctgattaca aaaaaataac ttttataagt tctataacgt
     2101 tgattatatt tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga
     2161 ctccttatat tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat
     2221 atgtacgacc gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa
     2281 attcaggaaa aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt
     2341 tttatacaaa agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata
     2401 ttatcggtag tcccatcttt cagattccat atattggact agtcctttca aaaacggcat
     2461 ttgcatggat tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat
     2521 ggaatacctt tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat
     2581 gttaaaaaca taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa
     2641 aacactcatg cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt
     2701 agtatgcgca atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg
     2761 cgtacccagc aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta
     2821 gcagaagtac cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag
     2881 cttcagcctg aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg
     2941 gccacgacaa tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc
     3001 gagaaagcta aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa
     3061 ggtcaagcag cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat
     3121 agtgcaaaat caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt
     3181 ggcgttttga tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag
     3241 attccccgtg gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg
     3301 gatcatcccg atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca
     3361 aaggaatcag aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt
     3421 gaatcgaggg ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag
     3481 gatacaggaa atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat
     3541 tgataacctt agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg
     3601 ggccaaaata attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt
     3661 aattatctat aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag
     3721 catccgcctt ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg
     3781 ttagaaactg aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc
     3841 aaaggcaaat aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata
     3901 aaactcatca gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta
     3961 gttgcaattg aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg
     4021 acaagtaggg tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg
     4081 tttaagaacc aaggagtgta caatggacta ttaggagtgg gactacttta tggagcttat
     4141 ctttcttccg catctaaaga aatcacaagt atgctattaa tatctatttt ttttgttgca
     4201 ttatatggca gtttgactag cgatcgttgg attttactca aacaaggtgg gctagctctg
     4261 ttggcattgc tttctatttt cttataaata tagaaaaaat aagaatttat ctaacaggaa
     4321 actttgtgaa aaatattact ttatctcatg aaatatgata ttctaatgaa tggtatttta
     4381 gaaaatttca tgaaaaagga gttagatagg tgttcaaaaa ttatgtttcc tcactaaaaa
     4441 aagaattttc aggttatgat ctttcaagat ttcagaaaga tcttttggct gggatcacag
     4501 tagctgctgt agcattgcct ttagccttgg cttttggggt atcaagcgga gctgatgcgg
     4561 ccgcaggttt gattacggct atcattgccg gtatcgttat tggcggttta tcaggcggtt
     4621 tttatcaaat atcaggtccg acaggtgcga tggcagctat cttgatgtct attgcggcaa
     4681 caaagggaat gcaaggtgta ttgcttgcta cttttttagc gggtctattt ttacttgcag
     4741 caggactttt ccgattagga acactgactt cttttatccc agcacccgta attacggggt
     4801 ttacttcagg tattgctatc attattgcat taggtcagat cgataattta tttggtgttc
     4861 actcggaagg tacaaacgtt attgaaaaac taagtagtta tcaaacgata ggatttccga
     4921 taaacacagc ctctttgatc atgggagggc tagtcatttt agggatgttt atctatccaa
     4981 aaaaatgggc gaaacgaatg cctagttcgc tgctggctat cattttggca acagcagtta
     5041 tgctattgtt ccatttacca gtagcaactg tgggtaagat tccacaaaca ttgatcagta
     5101 gtaatcgttt gaatatggga gattttagct tttctgcctt gcaacaagtg gcagttccag
     5161 ctattagcat tgctttatta ggaatgattg aaagcttgct atgtggtgct tcggctggcc
     5221 gaatggcaaa taagccctta gatagcaatc aagaattaat ggcgcaagga attggaaatc
     5281 tgttgcttcc tttctttggc gggattcctg ctactgcggc catcgcccgt acaagcgtgg
     5341 ccatcaaatc tggtgcacaa actagattag ctggtatgat acatgcagtt gtcttactgc
     5401 tctccatgct tgtttttgcg ccagtgatgt cacagattcc aatgccagca cttgcaggtg
     5461 ttttgattgt gacggcttgg cgtatgaatg aatgggaaac gatcaaggag ctatttacta
     5521 ataaatattg gtcagcagtt ctattgttta tccttacgat gatttgtaca gttatctttg
     5581 atttaagtat tgctatcgtg atcggggtga tcggcggctg tgtatttttt gttgtcaaaa
     5641 gcgctgctat cacgatttca ttagaaacga tcgattggca aagaatgggg ctgccagaaa
     5701 cggaaaagct gaataactgg acagtagtgt atattagtgg tccactgttt tttatgtctt
     5761 cagaaaaact caaaaataaa ttggaagaac taaatcatca cgatggtgtc attttctcca
     5821 tgcgaggcgt accaagtatt gatttgacgg cacgtgcagt ttttgatgaa tttcaagaaa
     5881 aagcagagca aagaggacaa acgattatct atacttcctt gcagccagaa gtagaaaaac
     5941 aactttctca cttgtgggaa catacattca cgcatcaaca tcaaacagtt gctcatgcgt
     6001 tgacagcttt acatgcgaaa tatactgcaa ttagccatta aaacaatgaa aaagaacaga
     6061 caagactgta aactgaagat tttatggtct tgtctgttct ttttaatatt gctgtaaaag
     6121 tgaagctagt acttgccagt cttgatggat aacaggtaca agggagcgat tgtagaaaat
     6181 agctgctgga tgaaaaagtg gaagaattcg atagtttttt tctgaccatt cgtaggcatc
     6241 cttttgttct gttaattgta agacaggccc tgtatagagt tgaccatgat ttttggtaac
     6301 cgtatattct ttgccaagta aacgttgaag accaatgttt ccaagtgtcg caattaattt
     6361 tggctggacc ttcatcagtt cgtaatccaa tattggtgca tgcgcaaata cttcggaacg
     6421 agtaggtgtc cgatttggat aaacagtttc tgactgttgt gtccgtttat tgatccgatg
     6481 agtcacgcga tacgggcggc tccttacagc actggtgata tacacctctt ctcgtgttaa
     6541 gccagttgaa gaaagtgcct gcatcaattc ttttccagct tgaccgctaa aaggaacatg
     6601 cgtatcaatc tctgttttcc ctggggcttc tcccacgagc atcagtttag gatgaagagg
     6661 tccttgccca gcgataaatc cttctacagg acggccgttc attcttaaag aagcttcttg
     6721 tatcagttct tttgggtatt ccatttatgc tcctcctttt tcttttatta taagaataaa
     6781 agaaacacaa tgcgactatt ttatttggta gaactgtgag gatagaaaag cagaagtatt
     6841 gcttgaattt ttgttttact ttttgatttt aaatttagtt gactaacggt tgctgttctg
     6901 gtaagctagt actgttgtaa ttgtggactc cacagctaca accgctcaga acaagtaata
     6961 agcacagcat atgctgtcac ttcggatggc gagtctaagt atatgagaag gaggtgcttt
     7021 atagcatgta cgcaattatt aaaactggtg gtaaacaagt aaaagttgaa gtgggacaag
     7081 caatctatgt tgaaaaactt gacgttgaag caggagaaaa agttgttttt gacgaagtta
     7141 tcttagtagg cggagaatct acaaaagtag gcgctccaac tgttgcaggt gcaactgttg
     7201 aaggtactgt tgaaaaacac ggcaaacaaa agaaagtcgt tactttcaaa tacaaaccta
     7261 aaaaacattc acaccgtaaa caaggtcatc gtcaaccata tacaaaagtg gttatcgacg
     7321 cgatcaacgc ttaatagcgt ttgagaaaga aggcctatat atgatcaaag gaacatttaa
     7381 gcgaaatgac gcaggtcaga tcgtttcatt cactttgacc gggcatgctg atgctggtcc
     7441 ttatggaagt gatatcgttt gtgcgggtgt ttctgcgtta gcgatcagca ctgtcaacgg
     7501 gattgcctca ttagccggtt ttgagccgat tgttgaaatg aatgaagaag aaggcggtta
     7561 tctttatacc gaagtcactt caggaatgac acaagaacaa aacaatattg ctgagattct
     7621 attagaaaat cttttattag gattacaatc gatagaagct gaaaactcag aatatatcca
     7681 aattaaaaca attaatgata aataggaggt gcagaccatg ttgttatcta tgaatctaca
     7741 attatttgcc cacaaaaaag gtggcggttc tacatctaac ggccgtgact cagaatcaaa
     7801 acgtctaggt gctaaacgtg ctgatggaca aacagttaca ggtggatcaa ttctttaccg
     7861 tcaacgcggt acaaaaatct atccaggtgt aaacgtgggt atcggtggag acgacacttt
     7921 atttgcaaaa gttgatggcg tagtacgttt cgaacgtaaa ggccgtaaca aaaaa
//
LOCUS       DACEDO010000097         7965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEDO010000097 DACEDO010000000
VERSION     DACEDO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.099"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..135
                     /locus_tag="IWO52_002573"
     CDS             <1..135
                     /locus_tag="IWO52_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0356804.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IWO52_002574"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IWO52_002574"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0356805.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="IWO52_002575"
     CDS             complement(2412..2663)
                     /locus_tag="IWO52_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0356806.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="IWO52_002576"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="IWO52_002576"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0356807.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="IWO52_002577"
     CDS             3613..3831
                     /locus_tag="IWO52_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356808.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="IWO52_002578"
     CDS             complement(4120..4632)
                     /locus_tag="IWO52_002578"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0356809.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="IWO52_002579"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="IWO52_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0356810.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7519)
                     /locus_tag="IWO52_002580"
     CDS             complement(7085..7519)
                     /locus_tag="IWO52_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0356811.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..>7965)
                     /locus_tag="IWO52_002581"
     CDS             complement(7529..>7965)
                     /locus_tag="IWO52_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0356812.1"
                     /translation="MVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKFAV
                     LGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAKMI
                     VRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
BASE COUNT         2215 a         1745 c         1425 g         2580 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catca
//
LOCUS       DACEDO010000098         7879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEDO010000098 DACEDO010000000
VERSION     DACEDO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.100"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..107)
                     /locus_tag="IWO52_002582"
     CDS             complement(<1..107)
                     /locus_tag="IWO52_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0356813.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSR"
     gene            complement(258..2960)
                     /locus_tag="IWO52_002583"
     CDS             complement(258..2960)
                     /locus_tag="IWO52_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0356814.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(2973..4262)
                     /locus_tag="IWO52_002584"
     CDS             complement(2973..4262)
                     /locus_tag="IWO52_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0356815.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            4412..5458
                     /locus_tag="IWO52_002585"
     CDS             4412..5458
                     /locus_tag="IWO52_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0356816.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            5460..7613
                     /locus_tag="IWO52_002586"
     CDS             5460..7613
                     /locus_tag="IWO52_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0356817.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2213 a         1601 c         1439 g         2626 t
ORIGIN      
        1 tgtcttgaac gattcaagca atgggcagtt ttagcaacag attgattcat tttgaaataa
       61 gactctatta ttacaagttc atccgttgta agatgggtat aggtcatttg tactcactcc
      121 ttataattct ttggtcggaa ctattttgag tgtagcacaa atgacttttt tagttgtcta
      181 gcttaatttt acaatcggcg tttctaaaat ttctccacca tctgaaaccg cacacacaaa
      241 ttttgttccg cctgcttcta ttccgccata cattcgtttt cctcctcgaa tccgattgtc
      301 cgaatctcat aaggttggat tattgggaca accgcttgct tcgttggttc ttcaagcaga
      361 ttcaatatga cagtttttcc attgtcttta gttatttcca atttttctaa atgactagac
      421 atattgaaac ctcttactac tactttatca ctaaacttgc tgcgtttcaa agctgtaatt
      481 gcaaacgttt cactttttat cgttaaatat tgttgttttg aaatcaatgt tccttcatga
      541 tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt atgcatgcaa ataagtagag
      601 aatttttctt ctggtccatg tagttcaatg ctgtaattga accgatgctc acctagacac
      661 tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt ctcccacact gcgaagtagc
      721 gtcactgcga tttgtccgtc ttctgttact tcatattcat tcaaaccaaa gtttccgact
      781 gttacaccgt actcttcatc atgaatattg acaaatgctt gctgatgttg cggattcgtg
      841 gggttttccc aacttttaga cacatggttt gggcgcttca ccacttcaaa aatactatct
      901 gcttcgtgat gttccacatg taactttgta ggaaacaaga cacgtaagcg atgatctttc
      961 atctgattgt cgagagtcgt ttcaaagtcg atttttttac tatcttttct gattgtcatg
     1021 attgttttta tttgaaggat tcgtttttct ttggatcgtt ctgcttttcg ataacgaaat
     1081 tccacaacca tctgctgttc tttttctaaa cgctcgtctg cagaaacagg gatttccatt
     1141 tcctgtagaa tacttatttt tgctatttcc ggtgtatcta ctaatacttt tacttcagag
     1201 ttttctttgt tactggatag gattggctga tcacagaaag gttgtttaaa gatatactca
     1261 tttcctatat cgccgatatc ttcaaaaata tgcagattat tcaactttcg tttgtttttg
     1321 cgatcttcca ttgtaatggt accatttttt tcaatcgtca aatgtagagg gccgttttcg
     1381 atgattcgtc cactctgatg aatcattgtt tccttttctt ctgctttcgc ttccccttct
     1441 acaagcgcga atgtttccca agaaaatgct ggcatgtttt ctaatggtaa agtcactttg
     1501 atcattcttg ccatatatgg cacacggaaa cggtctttcg gcaaatcata accaaattgg
     1561 acagtttctt cagataaaat agctggtata gtggccccac tttttgtttc tacatgatac
     1621 ttacgtttag gcaaattttc taactcttga tatagttgtt caggtattcc ttcagcgaat
     1681 ctttttcttt ctaattcgat ggttatttct gcttcaccag ttttatccat tcctgcagta
     1741 tttacaatga caaatggaaa gctgtctttt gggaatccgg tcgtatcgat tgctgcggtc
     1801 agctgttcta aggaatcttg tgcaagatac tttccaactt catctgcttt ttcaaaacga
     1861 ggtatcattt cccgatggac agaatcgact gaacaaccgc aaatactgtc gtgaggatga
     1921 ttttgcatta atgtcttcca agcataatct aattgatcat ggggatagtt tccactaact
     1981 tcataagcca tcgtcgcaag tggttctgta atattttcta gctgtcgctg gaccttggta
     2041 ttccattgtt ttaagtaaac ccgagcagat gctgtattgg ctaaagtata ccagccatcc
     2101 gtttcttgac ttgtcaattc tccttctact gagcctaagt cttctggcac atctttttga
     2161 actgcttcca gatagtcagt aaaattagaa tgaataaact cgtaatctgg aaacagttca
     2221 tttgcgagat ggattgcttt tgatatatct ttttgtaccg gttgatgatc aacaccattc
     2281 atcatcaata agtggttcgt agaggcatat tgttctgcat ccgctaattt ttgcttccaa
     2341 aaggcaagag ctgcttcttt ttctactgga atctcattcc cgttgctata ccaattggca
     2401 aatagcagac caaagattgc tgtttgatca ggtcctttcc accacatctc tgagtattgg
     2461 gaagaatagt tttctgcttc caaaacttga ttatcaaagc caattggttt tacaccacgg
     2521 ccaaacgctg ctgctgaaat accagcttgt ttcattaatt gaggcgtctg tcccatattt
     2581 ccaaatgtat cgggaaaata gcctaacatg acaggggttc cccatttacg gctttcttcc
     2641 atgccaatca gcatatttct cacgttcgat tccgagctga taagaaaatc atcttgcaag
     2701 atataaaatg gtccaattct caattttcct tcattgattg cttgttggat tgcctgtctt
     2761 ttttctggcc ttacttgaag atagtcatct aaaataatcg tttgcccatc caaatgaaaa
     2821 ctgttgaagg acggatcaac ttcaaatagc tctagtagat cgtctatcaa ttcaatcagt
     2881 cgcatgtgat gctgttcgta agccatgtac cattcacgat cccagtggct atgagaaatg
     2941 atatatactt tctttttcat ttacgatacc tcctactttt ctactctaat atcaaagtaa
     3001 tccatgacta attcgcaaaa catcatattt gcccaagaaa accattctct cgtataattt
     3061 tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg ttcctgcgtc agttgctaca
     3121 agtaaattca atatccgctc tttttcagat ttgtctgacg ttgtcattcc ttccatagct
     3181 aatgcaatcg gccaaacgta tttttctggc gtatgggagg atccaattcc ttttgcgtac
     3241 tttccttcat agaaatatgg attttcttta ctcaacaatg ttcttcttgt ttgcagataa
     3301 tcttcctctt gttcagaaca atatcccaag taaggtgcgg cgatcagatt gggcacatta
     3361 ctgtcatcca tgactgtggc gtttcccatt ccatctactt catatgcata aatcgtttgc
     3421 cctttttgat taaccgttcg tccgtgattc tgaatcccct cttggatctc tttcttcaat
     3481 ttctgtgttt gctgtacaat ttctttatca tcgagtatcg tttgaaaaat ttcttccaca
     3541 taacctaaca cgaccaccgc aaacatattg gatggtacca gatatccata acggcaagta
     3601 tcatcgctag gacggaaacc agaccatgtc attcctgtaa aagctgtttt tgcacctcgc
     3661 ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc gtacaaatgt ataaggagag
     3721 tgttcatgat cttgctctgt tttgaacacg tccaagattt ttttgatacc ttcaataaaa
     3781 gattgattga attgatcagt ttttcctgta tttttgtaaa gaagataggc caactgtatt
     3841 gggtagcata aagaatcaat ttcatatttt ctttcccaga tccaatcgtt catttttgta
     3901 tgatctgttt ggtggccagc accgttagct tcttcgttga aggcattcgc atatggatcg
     3961 atattgatat aaaaaaattg ctttttgact aacccgctga tcatgtcagc taattcctca
     4021 tcttcttttg caatcactaa ataaggacga acttgtgcag tagagtctct taaccacatc
     4081 gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat tttgcttttt gactgttgtc
     4141 aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc agtccgcatg gatatcccca
     4201 catttttgtg tgatatcttc cataaatttc tgcaccgatt ttggtatatc tttataagcc
     4261 actaataaaa ccttctttct atttgatata tctttaaaga ttatacttca tacagccaat
     4321 tatgtctacg ctttcaaaaa atatcttttt atttactttt tcatccatta tggtattatt
     4381 tgaatatgat atatcattaa gaggtgttta tatgaatcaa ccattgtacc agacaatatt
     4441 taatgatttg caaacggcta ttttaactgg tgaattacct gtagacagcc aactaccaac
     4501 tgaaaaagaa ctttcacgta cctataaagt aagcagaatc acttccaaac gtgcattgac
     4561 tgaattagag caattaggac ttatttatcg cgttcgcggg aaaggcagtt tcgttaaatc
     4621 cgcttctccg gaacagattc ttgcttctcc tacaaaaaca cgacggattc tgtttcttct
     4681 tccatatgtc gctgatttgt ctgttggtga ttttagcaag ggattgaacc ctgtgatgca
     4741 agataaacag attgatgtca tgatgacgac gcttgacttt ctacaaaata aaacagcaaa
     4801 agagctaatc aatgaatttg acggattgat ttattatgct cttgatacag aacaacatct
     4861 tgacttactt tttgagcttt cattgaaaga attccctgtc gtcatattag ataaaaaaat
     4921 ctatgagtta ccttttccta ccgttctttc cgataatttc caaggcggtg cgcttgctgc
     4981 ctcttatttg atcgaacagg gacattcaaa aatcgcttat ttgtttggta cgcagactca
     5041 tccacaatcc gttcgacagc gttaccttgg ttaccttgaa gcattggaca aagctggttt
     5101 gactttccac acttcactgg atgacaaaag cgcgacatta gatgaattgc tttcttatat
     5161 cactaagtat gaagtaactg cttttgtttg tgaaaacgat gttgtcgcta tccagaccat
     5221 gaatctcttg agacagcatg gttatcatat ccctgaagat ttttcaatta tcggatttga
     5281 tgatatccaa gcagcagctc ttgttgaccc gccactgaca accatcgcac aagattttga
     5341 acagttaggt aagcttgcgg gagatgcttt ggtcacttgg ttagagacaa aagatcgacc
     5401 atccgatagt aaatttccag tttcattcat caaaagacaa tcaacaaagg agaaataaaa
     5461 tgaatatcgg tcaaatagat actagacatg caacagcgaa tcagccaaat ttctctaatg
     5521 ggaactgcca gccttatact ggcgttcctt ttgggatgaa ttattttgca ccgcaaacta
     5581 cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg cgtttttcaa ggctaccgtc
     5641 ttacccatca accaagtcct tggatgggtg atttcagtca tatgctgttt acgccgatca
     5701 atggaaaact acaggagaac acccttttcc atgctcaaag ttcttaccgc ccagaagaaa
     5761 gtatattttg cccgactcat ctgtcgatct gtcaattacg ttatggcatc tgttctacat
     5821 tgatacctag catgtatggc ggaatcttat ctattgatta cagcaaaaat gacagcggtt
     5881 tacttttatc ctttccggga cgccatcagc tctttgttat agatccttac acagtggaag
     5941 gaaaaatcac acattttgct ggttcagaag atcctgattt ttctttttat tttatattga
     6001 gatttgaata tcctttaaca attgatgagt tagctgtttc caacaaaaat agcgactcga
     6061 tcccgttgta ttttgaacag acaaaaaaac aaactatccg atttgggaca tcttttatca
     6121 gctcggaaca agcagcttat aacctggaac tagaacaaaa ccaaagcaaa gagacttacc
     6181 ttgctcacag tcaagaacaa tggcagtcct attttgatcg tatacagctc acacaccatg
     6241 atcaacaaca attgcggacg ttttatcata atttatatcg ggtgttttta tttccacaaa
     6301 cattttatga aattgatcct agtgggcaaa aaatccacta tgatacgact agtcgatcta
     6361 taaaacctgg tgttctctat acaaataatg ggttttggga tacgtataaa acggtttatc
     6421 ctctttattc tttgattgct gtagaaaaat acgaagaaat gctggaaggt tttttgaaca
     6481 gctatagaga aagtggctat ttgccaaaat ggctgtctcc tgatgaacgt ggacttatgc
     6541 ctgggacatt gatcgatgcg gtcattgcag atgctgctgt aaaagatatc cgacgagact
     6601 tgatgcctga atttctaacg gcaatggaaa aaggtgctac ggttcaaagc aaacatccaa
     6661 attatggacg ccaaggaaca gaagattact tgacatatgg ctatgttcct aatcactacc
     6721 acgaatcagt caatcacaca ttagactatg cttatagcga tttttgtatt agccaagttg
     6781 ccaaggtact agaagaagag gaaatgacga agaaatatca gcagcaatca ttgaactatc
     6841 aaaatatctt tgactatgag tcaggattta tgcgttcaaa agataaagat ggcaagttca
     6901 gagaaccatt ttcagctaat cgttggggac aagattatgc agaaggaagt gcttggcaaa
     6961 gcagctttgc tgtttatcac gatttttcag gactgatcgg aaaatatggt ggcagtgaga
     7021 gatataaaga aaaactcatt gagctgtgca atcagccccc aacgtttgac ccaggaggat
     7081 atggttttga gatccacgaa atgagtgaga tggccgcgat agaatttgga caattagcta
     7141 tttcaaacca gccaagtttt cactatcctt acttgttcag ttatatagga caacctgaaa
     7201 tggcacagcc actgcttaaa cagttgatga ctcagacctt cgacgattct ccaatgggtt
     7261 accctggaga tgaggacaat ggaagtatgg ccgcttggta tatcttcaat agtctgggat
     7321 tttatcctgt cacgcctgga accggtgaat atgttatcgg tatgccatta attgacaagg
     7381 ctgttttgct tttgtcaaat ggaaaggaat tgacgattga agcaacgcct aatcggccgc
     7441 agcaacaatt tatccataaa atcaaacgga tggacaaaga atatacgaaa ttatatttta
     7501 cccatcaaga tttgctgaaa ggcggcacga tctcttacca attagggatc atccctcacc
     7561 ctaagaaata tcaagcaaaa gatttgcctt tttccttatc aaactataat taacaagagg
     7621 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat agatcctcat
     7681 atttttcgtt tggttggtct tattaccgca gatgcaagct tttgaacacc gtttattgag
     7741 aggttgtgaa atcagcgttc tgacctgagt attagagaga caaatggcaa aatagttcct
     7801 catattttgc cattttttga gctaataccg agggtcagct gattgaacac cgtttagtgg
     7861 agaggtcgtg aaaaagctg
//
LOCUS       DACEDO010000099         7689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEDO010000099 DACEDO010000000
VERSION     DACEDO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.101"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(22..3171)
                     /locus_tag="IWO52_002587"
     CDS             complement(22..3171)
                     /locus_tag="IWO52_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0356818.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3185..4402)
                     /locus_tag="IWO52_002588"
     CDS             complement(3185..4402)
                     /locus_tag="IWO52_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0356819.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4392..5987)
                     /locus_tag="IWO52_002589"
     CDS             complement(4392..5987)
                     /locus_tag="IWO52_002589"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0356820.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6112..6897
                     /locus_tag="IWO52_002590"
     CDS             6112..6897
                     /locus_tag="IWO52_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAQ0356821.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         2229 a         1570 c         1287 g         2603 t
ORIGIN      
        1 aattttctta actcttctcg tttacctcaa ttcattttca agtggaatca actcatcatc
       61 taacatttta cgccatgctc gcttcatttc ttgtgggtat ttaaatggtt tcgcttctgg
      121 atgtttggct ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat
      181 tggatcttca cctggcatat attgcagatt agaagcatgt aattctttct cagaaacaaa
      241 ccactcattg gaaaaggcat gaatcaaatt atcacttgct tgtgtaaaat agcgacgttt
      301 aatgacaaag atatcttcat tactatctaa agtccccaca tcaaatgcat ttaaaatttg
      361 tcggtaaact tctttgactc cttctgtttt attcaattta gccaaagctt tttcaatttc
      421 ttctcgtgca tctgaactat tcgcagcata agttcctagt aattgatcaa tataagtaga
      481 atcaatatca tacaacttag tggtattttc actataaaat tcgatcgttg aaaaatcttc
      541 tccctctttt tctggttctt cctccatcaa tgaacccttc acggtctcgt acacgccaat
      601 ttgttcttcc aaaagttgaa tttgattgtt caaagttgaa gattgcacca tgtcatcatt
      661 gtaatcatca taagtcacta acgattcata cgcacgattc aattcttgaa atgctttgac
      721 gtactcgata cggatttcaa gtggcgtatg ttcatcaatg gattccatgg atgaaacctc
      781 ttttaactta ccatatgctt gcttgaatct ttttttggat tcttggtaac ttggataaat
      841 caaacccgat tcttctttag cttctgaata cagttttgtc gcatcctcga cattcttctc
      901 cattgtcgct ggctttcgga aagtaacaat tagtcctttt gttttttcag gatacgtacg
      961 gttcgtacgt gaaaaggctt ggatcaatcc tgcatactct agattacgat ccacaaatag
     1021 ggtttgaatc gtaggggcgt caaaacctgt caatagacga tccactacga ttactaaatc
     1081 gatttgtttc ccaaattgtt tgaattccgc tctttttcgt gccaaacgat tattgatgtc
     1141 tccgttataa cgctcaatgt ctgctagtga ccaggcagta tcataatact gattatattc
     1201 ctcaataatt ttttgcattt cttcttgttg ggcacttgaa tctcggctat tttcttctaa
     1261 tgaataggta atagcgattc gtgggaaatc aggatcctcc atcgttcgtc cttcacggat
     1321 aggctgattg ggaaactctt ttattaacca atcagattct ttcgtcatct ctttgatggc
     1381 ccgataatac tttttggcca tatcaatgga actcgtggtt aagatcgcag acttcgttgg
     1441 acggccattt cgaaaatcaa attttgtata ggcattgtct ggacggaaca ttttatgaat
     1501 gactttttgg atatgttcct ctgcttcaaa aacagtaggg tccaaataac tttctttttt
     1561 gacagactcc atttgatcga tcatacgatt gatctgttcc ggtgaatact ctgcatatgt
     1621 ttctacttct tttaatttcc gatagatttt attctctaat gaagtcggct ccacggtgtt
     1681 ttcatgctct acttgaaatc ctaaaaccga gccatcttct aatgcattct tgatggtata
     1741 ggtgtgcaag acctctccat actgatcatg ggtcgtacgt gccaattgcc ctttggcttg
     1801 tttccgattc tcagggaaaa tcggtgtacc tgtaaagcca aaccaagtgg atttcggaaa
     1861 catttttttg atttcttcca tattttcagc acttaacgct cgatgacact catcaactac
     1921 aaacacgata tgttgcccca ttaatttttg gaagcgattg gttccttttt tctcttcctg
     1981 tttctttgca tatttcaacg ccgcatctag tttttgacgg gttgtaatga tcacaacatt
     2041 cgcattggca tctgccaaca gagtttcact taactctttg gcacttcctg ttcccacgat
     2101 caagctattc gctttggcat taccagaaga aaccccagta ttaaactccg aagcaaattt
     2161 cgtaaactct gtggtggtct gattatctaa atctttgcgg tccactaaca tgatcgttcg
     2221 atcgatcccc gattttcgtg ctaaaagctt agtagataca aaactagtca acgtctttcc
     2281 tgaaccagtc gcatgccaca cataccctga ttgatgttta tttgcggcaa taaacaaggc
     2341 ttgaatcgca tgcacttgat aaggatgtaa caccatgagg gttttattgt cttgatcttc
     2401 actgacaatc gtataatccg caatcaagcg atgggcatct gggatattta gcacttgttt
     2461 gcaaaactca tataagtttt ctaccttttt attatctttc gttcgccaac taaataaaaa
     2521 cttaggatgc aaatccttcg gtaaggcatt tgcaaaatac cgagtcgttt gttcattgga
     2581 aaccacaaac aactgtaagg tcgaaaagat attgttccga aacatgcctt cttcagcata
     2641 ttttttgatt tgattaaacg cctgataaaa accatctttt gcagttactt gttttagctc
     2701 gatttggata atcggtaagc cattgatcaa taaggtgacg tcaaaacgtc gatcccgcgc
     2761 ttctatgtta cttccacgtt ttgcaatttg atggaccact tcataagtag agattcctcc
     2821 accaatgtct tgattggaat aaaggataag tgagacagaa cccaattgcg gattttcccg
     2881 ttcaatcgtg atccgagcca tcccattttc accttttaac catttcgcag catcaaatgg
     2941 ggttttcgtc cgtagcaata attcggtttt gatcgtttca aactctttat cggtcaaagg
     3001 agaatcattc agttctgctt gattatttga gatgattttt tgacgtaaat tgctccaaag
     3061 atcttcttct gattttagat ccggacgata cgtccactga ttatgtcctt ccccgaggac
     3121 acgaatcaac tgttcttcga cttccgcttc ctcacggtga ctaattttca ttcttcttcc
     3181 ctccctaaac aaacatcttt tgcaagaagc cttttttcgt ttccttcagc aaatctaact
     3241 tacgttgatg aagagcgatc gtatcatcta gctgcttgaa gaaggcacca attttttgtt
     3301 gttcatttat atccggtgga aactgaaatg tcctttgttc tataccacct ttagtcacat
     3361 gtaccattgt cgttccgtta gttgattctt taattttctc tttatcagtc aatagccaag
     3421 tatatagata ctgtttgtcc aattccttag ataaaaattt aagtttccaa atatgataat
     3481 ggtagattac acgttcttca ttccacattt caggaccgaa gcttgttgcc cataaatata
     3541 agagatctcc tttattagca tacttgtctt cctctaattc taagtttgaa taataccatc
     3601 tatcattagt attaaaatta cctactctta aaaccctata ttttccagaa tcaagtaatt
     3661 cttgttgttt aaaagctcta ccattcaaaa aaactaccac ttcccccaac ttccgctctt
     3721 cccaatctcc cgtaaatcct ggaaaacgaa cttctggcac ttttgctccg tttttaggga
     3781 acattttttg taagaatcct tttttcgttt ccttcagcaa atctaattcg tgctggtgaa
     3841 gcatgatggt gtgatcaagt tgtttgaaaa atgaaccgat cttgatctgt tcgtcataag
     3901 ttggaagtga gataggtgtc tttttaccac tatcaattgt ataggttccg acggttccaa
     3961 tgcctgcaac gtcacggata tacttctgaa ttttgttatt tatgaacgag tagtaaaaga
     4021 agtttccgtc aattttatgc tcattcttga accaaatgat gtttccatcc ttaaagtaaa
     4081 ttgggttatc atcagtgatt aataagggga cacctattga tccgacacca gttacaagca
     4141 agtctccttg agaaacttta cctgaatttt tcgaatattc attatatttt tcttctgaaa
     4201 tgtaaagata atcggatggt tcctcatttt ttgcagctga gacaatatca cgagcacgta
     4261 aaaatctaat acctgagtta gtccaatctg attgatgaat tcgcttcaca gaagtaacat
     4321 ccatcatatc tcctaactta cgctcttccc aatcttccgt aaaaccagga aaccggattt
     4381 ctggtcgtgt atcattgctc atgtttgaac acctccagcg caccttgtaa ccacgcttca
     4441 ttttcaggtg ttgtttgcag ggaagagata gcttctaaaa gttctttttc tagcacttgc
     4501 tgttcttgtc gtatcttctt gatgtcattt ccgacatgaa ccatatcaat tggttcttct
     4561 tcttcgaaag tatcgacata gcgtgggata tttaagttat agtcattttc tttgatttca
     4621 tcaaaggttg ccaaatgagc gtatttctcg acatcttttc tttcagcgta ggtttcgagg
     4681 attttttgaa tgttttcttc cgataattta ttctgatttt tccctttaac aaattcacga
     4741 ctagcatcaa tgaataatac atctcgagtt tgacgattct tcttcaatac aatcaccgtc
     4801 gttggaatcg atgtaccaaa gaacaagttc gcaggcatac cgatcacagc ataaatactg
     4861 ccgtcttcta ataatttttg acgaatgacc ccttctgcag caccacggaa taaaaccccg
     4921 tgtggcaaga cgatagccat agtccctgtt tccttcaaat gatagaaacc atgaagaaga
     4981 aacgcaaaat ctgctttgga ttttggcgct aatttcccgt agcgattaaa ccgagaatca
     5041 tctaagaagg tcgtatctgc tgaccaatta gccgaataag gtggattcat cactactgca
     5101 tcaaatgtgt aaggttcatc tgttggccag tccttattca acgtatcccc attgcggaga
     5161 ttcatttctt cggcatctac cccatgtaaa atcaagttca tttttgctag attgtatgtc
     5221 gttgtattta gttcttgccc atgatattta acgttatctg gatgagttaa gtagttacgt
     5281 acatttaaca tcaaggaacc tgaacccatg gttgggtcaa agacactaaa gaatcgtcgt
     5341 tctttttgat ccaacgtcac gatttgtgcc atcatatcag aaaccatatg aggcgtgtaa
     5401 aattctccgg ctttcttccc tgcttctgaa gcaaattgac tgatcagaaa ttcatacgca
     5461 tccccgatca catccccatc gtgacctaaa acttccacgt cattcaattt cttgataact
     5521 tcggtaatcg tcacatttcg ttgttgctca tctgttccta gtttttttga ttggagatcc
     5581 acatcatcaa ataacccatt aaattgattg taggtagatg ccaattgcac aaaggctttg
     5641 tttagatcgt tcaattggaa cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga
     5701 tactctggtg agatcgaata ccccaaaata tcaacgattg tggcaatcag gtcttctttt
     5761 gattcttcat ctgaaagtaa ttcacgatac aacattgttt gtttgcttac tgtgtcatat
     5821 tcctctaagg attcatctgc taaaagaacc acttgttcca acaaacggtc ggataaatat
     5881 ttatagaaaa tcagtcccaa taaataattt ttatattctg acgcatccat tttactacgg
     5941 agattatctg ccgcactaaa taatttttga tttaattctg cacccaaaaa gatacactcc
     6001 tatctcttat ttaaagttgc tatataccct cttattttag catactccct tttagaaaaa
     6061 gcaaaaaaaa aagaaaaaat cttattcttg ataacaagct acgtgacgta tatgcctaac
     6121 aaaaatagaa cttttgaatc aatcttaaat gaaggtttaa ccacagaacc ggcatccatt
     6181 atttttgatg aagttatcga tgacgttgaa gatagtaagt atcataaaaa tttaaaagaa
     6241 cttttttatt ttgcactcaa caatggacca agtttgttca ctggtatatc agtacctgat
     6301 agtgataatc cattaattat ggcaaaaatt tacttaacaa aatggtgtaa taaatacatc
     6361 agggatagga ataacccagc attaaaaaaa cctcttaaaa catttggcga aaaagatgct
     6421 gctttaacta ctagagtagc agcaaatgct aatattgatg atcaaaaagt attgaatgac
     6481 tacttgcgtg gacatttttt atatatgagc gctgagaaca tgaacggaag tattttagag
     6541 gaatatttag ccgaagtgtt agaacctgaa ggttggattt ggtgcgccgg ctctgtatat
     6601 cgagctgttg atttctgtta tttaggaacc tcaccaattc ttttacaagt gaaaaataaa
     6661 tacaatacag aaagcagttc gagttcagct ataagagtag gaacaacaat tagaaaatgg
     6721 aatcgtttaa ataagtcgac aaaaatttct ggtttggata gccctatccc caattggaaa
     6781 gcattaatag aaatgactga agctagtaag gaactggcag ctaagttaac tgaaaatagt
     6841 tatttagctt atattaatga aaaatccaca cgagaattat ggacactcga tgactaacaa
     6901 tatccttatt agtatctatc cacgatacat ggaaaaaatt ataaacaaac aaaaaaactt
     6961 tgaatttaga aatttctatc ttacctcaga tccttataat atcttttgga tttacgaaac
     7021 aatccccacc aaagctttaa agtataaatt gattgtaaaa aatccaatta ctaaacttaa
     7081 gaaaaatcaa cactactttt taggagacga tgagtttgaa caattagttt aaaagggaaa
     7141 atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca tttatgttca
     7201 ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg gcttttttat
     7261 ttgtctcgct taattataca aacggcgaat tataaataac tcataaaaaa tattttgact
     7321 ttcaaaatca cttttgctac cagaagttga ggcgaagtgt tttaagcata gttgtaccct
     7381 agtttgaccg ttaaaaataa ataacgatcg ctagagtgca actatgtttg tttgccctac
     7441 cgtcagcgtc ggcttggcct ctcacatcca ttaccgtcgg gattatctcc gttacatacc
     7501 cttacaaaat acgccctgta agcattgcat gaaaccaccg tgcatttgca ccgtatttcc
     7561 gccctagccg ttcaaatcgg tcgctaccac cattcagaaa cacgtgcggt aatggaaggt
     7621 ttggaggaag ctatccagac cactaacgat tgtccctatc gtcgtacgtt tcactcagat
     7681 caaggttgg
//
LOCUS       DACEDO010000100         7598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEDO010000100 DACEDO010000000
VERSION     DACEDO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.102"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(136..426)
                     /locus_tag="IWO52_002591"
     CDS             complement(136..426)
                     /locus_tag="IWO52_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356822.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="IWO52_002592"
     CDS             complement(544..804)
                     /locus_tag="IWO52_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356823.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="IWO52_002593"
     CDS             complement(941..1354)
                     /locus_tag="IWO52_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0356824.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="IWO52_002594"
     CDS             complement(1810..2295)
                     /locus_tag="IWO52_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356825.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="IWO52_002595"
     CDS             complement(2434..2649)
                     /locus_tag="IWO52_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0356826.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IWO52_002596"
     CDS             2873..3673
                     /locus_tag="IWO52_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0356827.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IWO52_002597"
     CDS             3692..5560
                     /locus_tag="IWO52_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0356828.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IWO52_002598"
     CDS             5553..6044
                     /locus_tag="IWO52_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0356829.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IWO52_002599"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IWO52_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0356830.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="IWO52_002600"
     CDS             6604..>7598
                     /locus_tag="IWO52_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0356831.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DACEDO010000101         7507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEDO010000101 DACEDO010000000
VERSION     DACEDO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.104"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(525..1871)
                     /gene="gor"
                     /locus_tag="IWO52_002601"
     CDS             complement(525..1871)
                     /gene="gor"
                     /locus_tag="IWO52_002601"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0356832.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2005..3246)
                     /locus_tag="IWO52_002602"
     CDS             complement(2005..3246)
                     /locus_tag="IWO52_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0356833.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3264..4187)
                     /locus_tag="IWO52_002603"
     CDS             complement(3264..4187)
                     /locus_tag="IWO52_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0356834.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4408..6900)
                     /locus_tag="IWO52_002604"
     CDS             complement(4408..6900)
                     /locus_tag="IWO52_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0356835.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6907..7374)
                     /locus_tag="IWO52_002605"
     CDS             complement(6907..7374)
                     /locus_tag="IWO52_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0356836.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2069 a         1635 c         1201 g         2602 t
ORIGIN      
        1 agaaacaaat ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgaagga
       61 cagctgattg aacactattt gttcggaaaa agtgactaac tatgacacaa tttttttcac
      121 agtccctttt ttcacttgat ttgattcgta ccagcgaact tgacgagaac tttcttggtt
      181 tggtaactat atggtattct tttaacatcc tcgtttgctt cttctaattt atttatacaa
      241 agtcacactt ttttaaacat ctgttcttcg atacatttct atttctttta aacatcgata
      301 ccaatatttt gaacttaaga gtgcatacca aatggattca tttactcttc gtcagttacg
      361 aagataatca aaatctccaa cttccctact tatttcgttt tgagaattat cgaagcaatc
      421 ttacataaat aactaaaaaa caaaaaacca gaaaatcagt atactgattt tctggtttta
      481 catggtttgt tcaataaaaa gctgttggaa ctgttaatat taaattatcg catcgttacg
      541 aattcttccg ctcctgttgg atggatggct actgtattgt caaagtcttc tttagttgca
      601 cccatcttga tagccacggc aaaaccttgc agcatctcat cgacaccaac accaatagca
      661 tgtaagccga cgattctttc ttcttttccg acacagatca atttcatttc gcatttttgg
      721 cgataatcat ttaatgcaaa gtacattggg gtaaagcgtg aacgataaac tttcaattca
      781 ttttcgccat atttttccaa tgcttcttct tctgtcaaac caattgttgc aattggtgga
      841 tgcgtaaaga cgactgtcgg aacaagttta tagtcaagat aagaatcttt ctttccatta
      901 aataaacgtt ctgataaacg tctaccagct gcgattgcaa caggagtcag atctaattta
      961 ccgatcacat caccaacagc aaaaatatgt tcatccgttg tattttggta tttatctact
     1021 ttgataaacc cttttttgtc caattccaca tttgtatttt ccagacctaa tttttcggta
     1081 ttcggtacac gtccaccggc aaaaagtaca caatcaccag ttaaggtttc accattttca
     1141 aatagcaccg tatattcgtc attgttttta gtaatttctt tagcaatatg atgtgcgtaa
     1201 gtttttattc cagcttcttg gtacatttgg atcaaattgt ccgaaagcat tttatcaaac
     1261 gttctaagtg gtcgttcttt tcgatatgcc cacatgactt cactacctaa accacaaaat
     1321 acacctgaaa gttcagccgc aatgtatcca gcacctaaaa cgattgctct cttaggtaac
     1381 tctgttaatt caaaaaatcc atttgaatca attgcgtatt ctccaccagg aatggtcatt
     1441 ttacttgcac ggcctccagt agcgatcaag attttaggag cacgataggt ttcaccgttt
     1501 acttcgatcg tttgactatc gataaaagta gcgtaacctt ttatagcctc gacatgattg
     1561 ctatctaatc ctctttgata agcaccgtgt agaaaatcaa tatatttttc tcgtctttct
     1621 actagaccgg cgaaatcaac attttttaaa tctgcttgga taccgtaact tgaggcatct
     1681 cgttcaattg tttccaaaat cgtacttgct tgccacataa cttttttagg tacacaaccg
     1741 acgttgacac atgttccgcc caattcattc gcttcaatca gcaaaacttt tgcgccatgc
     1801 atccctgctc tgtttgctga agcaataccg ccagagccgc caccaattac gatgtaatca
     1861 tatatttgca ttttgttcct ccttattgaa gagatcaatc aaaaccttta tttaacgatc
     1921 ctttttctac agaaaacaag tgtaacactt ttcttgaatt ttgtatgcga agatgcttaa
     1981 aacagggata tatcagcttt ttccttattt acgttttcgg atcatatctc caggtttcac
     2041 cggctgtgcg actggcatag ttaaaatcat cattggattt ggtgctcggt caatggattc
     2101 gccttcttca ttatgcataa cttcaacggt ttgactgaaa tgactgaatc ctggaccata
     2161 aaattcgatt tcatctccta cagaaaacaa gtttctctga cgtatcgtag caatctgtgt
     2221 tgcctcatca taagataata cttcaccgac aaatttatat actgggattt tacgacgttc
     2281 accgaataat tgttcgtttt cagttggtgt attgtagtaa aaacctgtag ctaattctcg
     2341 ttgtgctact ttccataact cgtcgatcca ttcttgcttg cagacatagt tttcaggatc
     2401 ttccatataa ctgtctacag ccgctttata gacatttgac accgtagata cataatggat
     2461 cgatttcatt cttccttcga tcttgaagct gtctacgccg ttttcaatca attctggaat
     2521 atgttcgatc attgacatat ccactgcact catcgagaat tcttcttcca cttcgcctgt
     2581 atcagttaaa ctgtttcgtt cttgaccaaa aggcatatcg aacaatccat acttccagcg
     2641 acaagattga gaacatccgc cacggtttgc atcccgcatc gacatatggt tggacaatgt
     2701 gcatcgacca gaataagaga tacacatggc accatggata aatgcttcga tttcggtatc
     2761 cgtattttta cgaatctctg ccacttcctc cattgaaact tcacgagcta aaacgacacg
     2821 ttccaaccct tcttctttcc agaattccaa tgtctcaaaa ttagttgctg aagcttgagt
     2881 ggataaatgg atcggcagac ctggggcttc tgtcgcacaa atttcgatca aagctggatc
     2941 agaaacaatc actgcagaaa taccgacatc acgcaaactg cggaaaaatt cccctgctcc
     3001 atcttcattt ccttcatggg tgaccatatt agcagcaacg tataccttcg catcatgttc
     3061 ttttgcaaaa gcaactcctt cagccatttc ttcataagta aaattacctg cacggcttct
     3121 taaaccatat gcattcccgc caatatatac tgcatctgct ccataatgga tcgcggtttt
     3181 taacttttcc agtgttcctg ctggcgctag gatttctggt cgtttcaatt ttctttttac
     3241 tgtcatttct ttcatcatac tccttatttg atagcagaag gatcaatata aaagaatcct
     3301 gtatctaatt cacggttttc aggatgcaat ttactgattt cgctagtcat tttttccgct
     3361 ttttcttgat cccagctacc ttctaaaatt tgtttttttg cttcggcgaa tagtcgagtg
     3421 atgtttacga atttttcgcc tggagtaaat aatccgtcca atttccatgt acagtaatga
     3481 tgctgataaa gttcattcaa ttcattcgag agattcagat cattgctggc aaaaatatgt
     3541 gtgccatgac tatcttcata aatagaataa tgtgtctctt cttttttagg ttccgagata
     3601 aaaagacctc tttcacgatc tttttgttcg tcttgtttcg tatagttata gtaattctgt
     3661 agcagtggac gtttggattg atggatgcat gtagcaccat acacaagggt ttctacagga
     3721 atatcgagtt tttcttccat cgccttcatc tcttcaaaag gaacttcacg agcaagtact
     3781 gctccaactg cgcctttttt cgcccagaaa ttgatttgac gtgcgctcgt tactaatgtt
     3841 tcagcatcat aaatatacgg tatacgcaat tcaggatttt ttttcatcgt ataaataata
     3901 cctgtatcac caactgtgat cacatctacg tttatttctt ttaaaaaagc caaatattca
     3961 ggtacatttt tcattttttc aggatgaatg atcccattta cagcaatatt cgctttttta
     4021 ccatacttgt gtgctaattc taccaattgt cgctgttctt ctcgtgtaaa tgaagcaggc
     4081 agacgcaatc caaattgctc ttcaccgaaa tatagtgtat ctgcacctgc agcaagcaac
     4141 tgttctgctt gttcaatagt ttctactgtg gttatgagtt cgatcataat tttctcctcc
     4201 tacaacttca taatagtagc atagctctaa aaactttcca atacttcttc tatttaaagt
     4261 cggatttttg aaaattattt cacaaaaaat caaaataaag ttcgatatat tctgtccaaa
     4321 tataaaaaga gattgtgaca aaaatcttgt cacaacctct taatcaagtc ttactattat
     4381 aaaaattcag ctacacaggc caagtaacta tagggaacct actgtttcct ttttaggtcc
     4441 gcgaacattc aatgtgatct taccttttga agcacctact gttacgtggt cacctaaacg
     4501 aatttctccg ccaagcaaag cctcgctcaa tcgatcttca atttcttttt gaagtgcgcg
     4561 acggattgga cgtgcaccat attcaggatc aaatccagct ttcccgatca catcgattgc
     4621 agatggtgtg attttcactt gaatatcttg ttctttcaat cgtttgataa tcgctttgct
     4681 catgatttgg acgattgtat ggatttcatc tttactcaat gaatggaaga cgacagtctc
     4741 atcgattcga ttcaagaatt caggtcggaa agctttcttt aactcttcta agatacgttt
     4801 ttgcatggct ttgtgatctt tagtaacatc ttggacattg aatccgacat ttttttcttc
     4861 tcgaatttgt gtcgctccga tatttgaagt cataatcatg attgtatttc taaagtccac
     4921 ttttctacct ttagaatctg ttaaatgacc atcatccaat acttgcagaa gtaaattgaa
     4981 aacgtctgga tgtgctttct ccacttcatc taagagaatc actgaatatg gtttttgacg
     5041 aattttttct gtcaactgtc caccttcatc ataccctacg tatcctggag gagacccgat
     5101 taatcggctt gtactgtatt tttccataaa ttctgacata tcaacacgga tcagggcatc
     5161 ttcacttcca aacatgactt ctgaaagtgc tttggccaat tctgtttttc cgacaccagt
     5221 tggtccaagg aacatgaatg aaccaattgg acgatcagga tctttcaatc cgcttcgtgc
     5281 acggcggatc gcacgtgaaa ctgctttgac agcctcatct tgaccaacga cacgttcatg
     5341 cagaagtcct tccagttcta acaagcgttc gctttctttt ttctccaact gctgtagagg
     5401 gacaccagtc cactccgata caactgtagc aacatcttct tctgttacac gatcagaata
     5461 accttgctcc tgttttgctt caattgcaga aactttttgc agctttttac gtagtgcttt
     5521 ttcttgtctg cgtaattgag cagcattttc aaagtcttgt ttttggatcg ctgcttcctt
     5581 ttcttgtact agttcatcga tttctagttt gatcacagtt gattttgtta agtgatcggt
     5641 ttgatccaaa cggactttcg ctgctgattc atcgatcaaa tcaattgctt tatcgggtag
     5701 ttgacgagaa gtgatataac gaacagaaag attgactgct gctcgtacag cttcatctgt
     5761 gatctccact ccatggtgct cttcatatct tgtacgtaat ccttgaagaa tcacttctgc
     5821 ttcttcagga gttggctcat ctacttgaat gcgagcaaaa cgtcgctcta atgcagaatc
     5881 tttttcgata tatttttgat attcatctag ggtcgttgct ccgatcgttt gtaattctcc
     5941 tcgagctaac gccggtttca agatattcga agcatcaatt gctccttctg ctccacctgc
     6001 accaattaag gtatgcaatt catcaatgaa taagattact tgaccatcat tatagatttc
     6061 gtcgatgacc tttttcattc gatcttcaaa ttcaccgcga tatttcgtac cagcaaccaa
     6121 agatcccata tcaagcatca tcaggcgctt ttcttgcatg tcttctggca cttcacctaa
     6181 aatgattttt tgagctaacc cttcagcaat ggctgttttg cctacaccag gttcacccac
     6241 cagtacaggg ttattctttg tccgtcggct aagaatctgg ataagccgtt tcacttctct
     6301 cgaacgacca acaactgggt ccagcttgtt ttcttttgct aatttcgtca aatcacgtgc
     6361 taatgaatct agcgtaggtg ttccttgcgg tgtttgccgt ttatcttgta catttacatt
     6421 tctacgacga ttcattcctt ttccagcaga atcaccagct agccccattt tttgtttcag
     6481 aacttgacgg gcttttgaaa ggctgatacc taagttgatc atgattcgag tagccatgat
     6541 actttcatct tttaataagc tcatcaaaat atgttctgtt ccaacttttt gagaaccagt
     6601 tttcttcact tcgctgctag ctaaagcaaa aacttgtttc attcttggtg aatatggaag
     6661 aaagactcct ttagggtaag aacggatacc accataacca atcagatgtt caatttcttc
     6721 ataaatttcc gtttcattca aatccatttc acgtaatact tttccagcaa ttccattttg
     6781 ctcgaccact aaggctagta aaacatgttc tgaactaact gattgatgtt tcaaccgttt
     6841 tgcttcttct tgtgcaattt ctaatactgc ttgggctctt tcagtaaaaa gttcttccat
     6901 ctaatctcac ttcccatctt cataacttaa acgttccaga aatgctcgga gaatccgagc
     6961 acgtgtatga tcttcatatt cattgaaatt caatgtgttt tttgcaattg cactcaacat
     7021 caagtttcct tctttttttg taattatttg gtcttcataa agtttttgaa taatagcaaa
     7081 agcatttttc tcactgattg tatcatcaaa taactgattt atctgctcaa gtaattgttt
     7141 tttatctgaa atccttactt ttgcaattcg tatataacca ccaccaccac gtttgctttc
     7201 tactagataa ccacgttgga tggtaaaacg ggtattaatc acatagttga tttgtgaagg
     7261 aacacagtta aagaggtctg ccatttcaat ccttcgaatc tcgatcattt cactattttc
     7321 cagaattttc tttatatatg attcaattaa atccgaggta ttctgatgtg ccatcttatt
     7381 cattcccttt gactaatatt gaccttaatt ataacgaatt tgataataga aagaaagaag
     7441 aacgcttttt gttaaataaa tcctttagaa tcaacaaaaa aaaatacgga atatactatt
     7501 ccgtatc
//
LOCUS       DACEDO010000102         7454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEDO010000102 DACEDO010000000
VERSION     DACEDO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.105"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..1751
                     /locus_tag="IWO52_002606"
     CDS             273..1751
                     /locus_tag="IWO52_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0356837.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2028..4088
                     /locus_tag="IWO52_002607"
     CDS             2028..4088
                     /locus_tag="IWO52_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356838.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4171..4740
                     /locus_tag="IWO52_002608"
     CDS             4171..4740
                     /locus_tag="IWO52_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0356839.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4915..5721)
                     /locus_tag="IWO52_002609"
     CDS             complement(4915..5721)
                     /locus_tag="IWO52_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356840.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5859..7373
                     /locus_tag="IWO52_002610"
     CDS             5859..7373
                     /locus_tag="IWO52_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0356841.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2674 a         1227 c         1452 g         2101 t
ORIGIN      
        1 aaagagcagg acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa
       61 tcttgtacat tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc
      121 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
      181 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      241 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      301 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      361 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      421 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      481 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      541 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      601 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      661 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      721 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      781 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      841 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      901 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      961 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
     1021 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
     1081 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1141 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1201 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1261 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1321 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1381 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1441 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1501 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1561 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1621 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1681 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1741 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1801 ttttttcaaa gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa
     1861 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
     1921 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
     1981 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
     2041 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
     2101 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
     2161 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
     2221 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
     2281 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
     2341 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
     2401 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
     2461 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
     2521 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
     2581 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
     2641 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
     2701 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
     2761 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
     2821 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     2881 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     2941 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     3001 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     3061 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     3121 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     3181 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     3241 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     3301 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     3361 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     3421 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     3481 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     3541 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     3601 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     3661 cccatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     3721 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     3781 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     3841 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     3901 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     3961 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     4021 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     4081 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     4141 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     4201 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     4261 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     4321 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     4381 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     4441 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     4501 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     4561 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     4621 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     4681 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     4741 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     4801 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     4861 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     4921 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     4981 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     5041 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     5101 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     5161 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     5221 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     5281 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     5341 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     5401 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     5461 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     5521 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     5581 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     5641 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     5701 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     5761 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     5821 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     5881 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     5941 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     6001 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     6061 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     6121 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     6181 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     6241 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     6301 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     6361 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     6421 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     6481 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     6541 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     6601 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     6661 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     6721 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     6781 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     6841 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     6901 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     6961 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     7021 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     7081 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     7141 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     7201 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     7261 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     7321 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     7381 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     7441 gaacactgtt tagt
//
LOCUS       DACEDO010000103         7370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEDO010000103 DACEDO010000000
VERSION     DACEDO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.106"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1152)
                     /locus_tag="IWO52_002611"
     CDS             complement(<1..1152)
                     /locus_tag="IWO52_002611"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0356842.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1337..3778
                     /locus_tag="IWO52_002612"
     CDS             1337..3778
                     /locus_tag="IWO52_002612"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0356843.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4108..4812)
                     /locus_tag="IWO52_002613"
     CDS             complement(4108..4812)
                     /locus_tag="IWO52_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356844.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(4999..5244)
                     /locus_tag="IWO52_002614"
     CDS             complement(4999..5244)
                     /locus_tag="IWO52_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356845.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5261..6112)
                     /locus_tag="IWO52_002615"
     CDS             complement(5261..6112)
                     /locus_tag="IWO52_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0356846.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6278..7282
                     /locus_tag="IWO52_002616"
     CDS             6278..7282
                     /locus_tag="IWO52_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0356847.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         2322 a         1324 c         1523 g         2201 t
ORIGIN      
        1 tattttgtag taacgaatat cattttcgat ctttgtaatc gaaaaaggta catctgaagt
       61 atattctgca cattctccgt gttcaggaag cgtctcactg aaaatcgttt catattcgtc
      121 aacagtgatc cgtgttcgag aatctagcat ctcttgatga gattgtgcga agagatattc
      181 ttgatagttt tcttccagat aacctgtaaa aaattcactt actgcaccag aaccgtagct
      241 gaatagaccg atgcgatctc ctggctgtag tgatttcgaa ttttccagta gagaagttag
      301 ccccaggtat aatgaaccag tgtaaagatt accgattcgt cggctgtaac ggatgctttc
      361 ttcatagcga gccataagac gttcctgatt ttcttcgtct gtttggtcta atacgctttg
      421 caatgccttt tttcccatct tagtatacgg aatatggaaa gcaatcgcct gataatcttc
      481 gagtcctcga cccgacaatt ctttatgtcg attccaaact ttttggaatg attcgatata
      541 cgtagaatta gataaaggac catcaacaac aggaaattcg ctataatctg gacgccagaa
      601 atcatagata tcttctgtca gaaatacgct gtcgtcttca atcgataaaa tacgcgggtt
      661 ttgagtgatc atcatcgcaa cagcaccgac accttgcgtc acttcaccac cgcttgccaa
      721 gccgtaacga gcaatatcac ttgctatgac tagtactttt cgttctggat gatttttgac
      781 atattctttc gccatgtgca aagctgcggt gccaccgtag caagcctctt ttacttcaaa
      841 agaacgagca aaagattgga ttttcagtaa atggtggata attaccgcac tcgcttttga
      901 atgatcaatc ccggattctg ttccgacgat gaccatgtcg attagctcac ggtctttttc
      961 tgttacgatt ttgctggcag catttgctcc tagtgtaatg atgtcttcat ttgcacgatt
     1021 gactgccatt tggtcttgac caatccctat atggtatttt gcaggatcat ccccacggct
     1081 ttctgccaac tctgtcatat ctaaatataa attaggaata aaaaaggaaa gacgatcaat
     1141 ccctattttc atttcaacac ctctttactt atgataaaaa acttacttta ctaaggtatc
     1201 ataaaaagat taaaagaaat ataaaaaaga gaatatttaa ggattgttaa aatttaatct
     1261 tagaaaaaaa taactggaac aagagctatt tgtttttaca gcaaagaatg gtatattttt
     1321 gtaggaggtg gcttacttga aagaagtcgt tatgatagat gctgcaagaa cgccgatagg
     1381 aaagtatcgc ggaagtttaa gtccatttac tgcagttgaa ttaggcacac ttgtgactaa
     1441 aggattatta gataaaacaa aactgaaaaa agataaaatc gatcaagtaa tatttggaaa
     1501 tgtcctgcaa gctggtaatg gtcaaaatgt tgccagacag attgctttga acagcggtct
     1561 ccctgtagat gtcccagcaa tgacgatcaa tgaagtttgc ggatctggta tgaaagcagt
     1621 gatcttagca cgccagctga tccaattagg tgaagcagaa cttgtgatag ccggcggaac
     1681 agagagtatg tctcaagcac ctatgctgaa acagtatcag tcagaaacaa atgaatatgg
     1741 tgaaccaatt tccagtatgg tcaacgacgg attgactgac gcattttcaa atgcacatat
     1801 gggattaacc gcagagaatg ttgcaacaca attttctgtg agcagagaag agcaggatcg
     1861 ctatgccttg tcgtcccagt tgaaagcagc acatgctgtc gaagccggtg tattttctga
     1921 ggagatcatc ccagtcaaga tttctgatga agacgtgtta tctgaggatg aagcagttcg
     1981 tggaaatagt acattggaaa aactgggcac gttacgtaca gtattctcag aagaaggaac
     2041 tgtaacagca ggaaatgctt ccccgttgaa tgacggtgcc tctgtggtga tccttgcatc
     2101 caaagaatac gcagaaaata ataatctgcc ttatttagca accatcaaag aagtagcgga
     2161 ggtcggtatt gacccaagta tcatgggaat cgctccaatc aaagccattc aaaaattgac
     2221 agatagatca ggaatgaatt tatcaacaat cgatcttttt gagatcaatg aagcatttgc
     2281 agcttcttct atcgtagtta gccaagaact gcagttagat gaagaaaagg tcaatatata
     2341 tggaggagca atcgcattag gccatccaat tggtgcaagc ggtgcacgta tcttgactac
     2401 attagcttac ggattgcttc gtgaacagaa gagatacggt attgcttcat tatgtattgg
     2461 tggaggttta ggcttggcag tcttgttaga agcgaatatg gagcaaacac ataaggatgt
     2521 gcaaaaaaaa aagttttatc agcttacgcc atctgaacgg cgcagtcaat taatagaaaa
     2581 aaatgtattg acacaagaaa ctgcgttgat ttttcaggaa caaacacttt ctgaagagct
     2641 ctccgatcac atgatcgaaa accaagtgag tgaagtggaa atcccaatgg ggatcgcgca
     2701 gaattttcaa atcaatggca agaaaaaatg gattccgatg gcaacagagg aaccatctgt
     2761 cattgcagct gcaagtaatg gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca
     2821 gcggctgatg cgagggcaaa tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa
     2881 tgcagttaat catcgaaaag aagagctgat tctttgtgca aatgaaagtt atccttcaat
     2941 cgtgaaacgc gggggcggtg ttcaagatat ctctactcgg gaattcatgg ggagtttcca
     3001 tgcatactta tctattgatt ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat
     3061 caactcgatt cttgagagtg tggcaaacaa gttacgggag tggttccctg aagaagagat
     3121 tctattcagt attttaagta attttgctac agaatcattg gcttctgcct gttgtgaaat
     3181 cccattcgaa cgcttaggga gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca
     3241 acaggcaggg gaatatgcga agcttgaccc ttaccgtgca gcaacgcaca acaaagggat
     3301 catgaatggt atagaagctg tagtggcagc tacaggaaat gatacacgtg cggttagcgc
     3361 ttcgatccac gcctacgcgg caagaaatgg attataccaa ggattaacag actggcagat
     3421 caaaggggat aaactagttg gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg
     3481 agcttccaat atcctgccaa aagcaaaagc atctttagct atgttagata ttgattcagc
     3541 caaggaacta gcacaagtaa ttgctgcagt agggttagct caaaacctgg ctgctttgcg
     3601 tgcattagtt acagaaggaa tccaaaaagg ccacatggga cttcaagctc gttcattagc
     3661 gatctcaatt ggggctattg gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc
     3721 agaaaagatg aaccagcaga cagctataca aatattggaa aaaatccggg aaaaataaaa
     3781 aagcaatatc tttagaagaa taaagcaaga tttacagtta ttttatttac tgcagatctt
     3841 gcttttttct ctatcagaaa atatgtttat tattagaatt ttttatagtt aaggttaggc
     3901 atcttaagaa atcaggcaac tccaagatat cttcaggtag aagagaagtc gcctaacatt
     3961 gtaggatcat aggtgatttg ttggacaggt caagctgata tttgagcctg atgttagaag
     4021 gaatttcttt ttacttgctg tagaaattat tatatgacta aaaaggctgg gattttaaat
     4081 cccagccttt tatgacaaat agaattttta tcgatcagta atcgctgtcg ctgcaaattg
     4141 ttcgatcaaa ggaatcaaag tcaatttgac tttgtatttc cgttcgagtg ctttttcgat
     4201 aacgaactca ttttcagtag tcatagaaat agttgctaat ttacgaggtt catcagcatt
     4261 tttttcatta ataactactt catgttcatt ttgttctgtg taaaatttag gatactcagc
     4321 aacggctttt gaaaaatcag caaaacttaa agcggcattc gtatcagttg agcgtgtatt
     4381 ttcagttgtg tccgcttttg gtttttccgt tttttcttct ggcacactga ctgtttcttc
     4441 tgtaactggt gtcgcaacag cttgcgcagt tggttcatcg tttttcaatc gttgatcatt
     4501 ccagtaagac acgatttcaa taagaagatt gtcacggaat cgttcgaaaa tcccgttttc
     4561 tcctgaacga attctcttta atacagtatt gaagttgtca tcgattggca cgttgatccg
     4621 tgtatcttca tttgttttcc cagctaatcc gatcgttaag atttgacggt ttttgggatc
     4681 aagcatgact tgtaacggca aaccataacg tcgcaagctc tcttcttcta gagaacggaa
     4741 taattgtcct cttacaccat ccaatgaagg acggaattga tttaataatt tttgtttctg
     4801 ttttttcttc atatgttctc tccttcgctc ataccctttc gattataaga aaaaaacagc
     4861 caaatgaaaa ggagtaatgc aaaaaaactt gcgtatacgc aaattacaac agaaaaattt
     4921 gcgtattttc ccgttttttg aaagaaaaat aaaaaatgga acggattatg tatatataca
     4981 taatccgttc cattctttct attttttcgg tatcaaggca aaaacaggta gttcaggtga
     5041 ataaatgact tcatgttcgt gccagggttt gaccattagc gaatgatcga accgtttttt
     5101 cagttcttcg atgatttgat catgtgtcca gtttcttgca ggaaaatgtt ggattttctc
     5161 tgcatcgatc aaaaagacaa aatcggaatg aaaagtctgg gtaacttctt ccgagagaga
     5221 attcaagcgt tcgattggat ccataaaatc acctttcgac ttaaaattgg gtagtatctg
     5281 ggtcttttgc tttgccgact acaccatcta actgatcgat ttgtttcatt tcttcttctg
     5341 ttaattcaaa atcaaataat tcagtatttt ctttgattct gcttggtgtc actgacttag
     5401 gaagaggaag gaattcgtgt tgcagcgacc aacgcaaagc aacttgtgca atcgtcttgt
     5461 catgtgcttc agcgatttgt ttcatttcag gcacatcgaa gattttacca gtgcctaatg
     5521 gactatatgc ttcgaggatc atatcatgtt tttttgcata ctcaactaca gttgtctgta
     5581 attcgcctgg tgcaaggaaa acttgattga ccatcggcat aatttttgct gtttttgcta
     5641 gtgtatctag atggtggggt aagaaattgc tcacaccgat tgctttgatt ttacctgctt
     5701 catacagttc ttcaaatgct cgccatgtat ctgcatttgc ttgttcccaa ttatcacgaa
     5761 aagcaacagg gtttggccag tggatcaaaa acagatctaa ataatctgtt tggagcttct
     5821 tcaatgattc ttcaaaagaa gacatcacaa gttcatagct gtggtttgca ttccataatt
     5881 ttgttgtcaa gaatatctct tctctaggaa ttccactttc cttaatcgct tggccgacgc
     5941 tttcttcatt cttgtatcct tgggcagtat cgatatggcg ataacctgca gccagcgctt
     6001 ctttaacaga ggaaacagcg acatccccat caggtgtttg ccatgtgccg aatcctacta
     6061 ccgggatttc gtaaccattt gatagacgat aagtgcttgt taatgaattc atactttcag
     6121 ctcctttttt attcgttcaa ctataagtgt agcgcatttt tcgaaaaaaa gaatccattt
     6181 tgtttctact cttgaaattt ttgtttaaaa gaaaatgatt ttgattctgg tatttttgat
     6241 tttttttccg taaactaaga cagaagggaa tgagaaaatg gatatctatt taaaaaaagc
     6301 aattctgcat attattgaca gagaaactgg ttctccagtc ttttcacaaa aagagctgga
     6361 tctgacaaaa gaatatattc gtgattttct tcaaaagaag atccaaaaaa tctcttctgc
     6421 tcaaacaaaa actggacaac tagcagaaga aagcatgatc gttgaatcac ttaaaatcgc
     6481 tcaagaaaat tttgtggaag caagtgaagc aatcgttcag cggtggttcg atatctacca
     6541 agaaagtgaa gaagcaccta gtgcagatgt atttgttgtc ttgtatgaat tagatacagt
     6601 gatgtactta tctttattga aagtcaatta tcgtgaggct tacactcact ttgttgaagc
     6661 agacgaagca gggatcgaca accgcttgat tttgaatcga gcaattctag gggcgaaaac
     6721 acaaaaagca gatgaagcat tcgctgtgaa tttgactgat tggacctacg aattaattga
     6781 aaaaaaatat gagttttctg gcaaaaaaga gctttatttt tctggaagag tgatcgaaag
     6841 tgttcctgca ccttctttag aagaaaatgt aaaagtgatc caaaaagtag ccaagcatct
     6901 tggaaaaaaa tttgagacag aagaatttga tatcatggct gatttgaaag aagctgtcta
     6961 tgatacgata gaagaaaaag gtcggttgga tcatgagatg attgcggaaa aagtcttcaa
     7021 ggaaaacatc acggcaaaaa tggcttttaa agaagaagta caagagcaag gatttgtccc
     7081 agaagcacct cctgtaaaag aagtacaaga aatttctgag aaaaaatttg gaaaacaaaa
     7141 attcaagcta tcaaacggga tcgaactgat cgtaccagta gatgtatatc gcaacccaga
     7201 tttgattgaa tttgtgaata atccggatgg tacgatatcc gtgatgatca aaaatgtgga
     7261 tgaagtattg aatcgtttat aaaaatgact agaatattag gtgttcaagt accagtgatc
     7321 agttaaatga attgtgtcaa gagaaggatc ggaaaagacg aatataacaa
//
LOCUS       DACEDO010000104         7261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEDO010000104 DACEDO010000000
VERSION     DACEDO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.107"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..2761
                     /locus_tag="IWO52_002617"
     CDS             <1..2761
                     /locus_tag="IWO52_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0356848.1"
                     /translation="KEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGEFRNFLVS
                     SSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFVLVKEEEP
                     AAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKKSYEEKTI
                     LINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQKSSEQTKK
                     LAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQDAEQLTIS
                     LDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEKQQEIEKQ
                     KAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARLQLFLRPG
                     EPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSEAHENNLQ
                     QQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSLDRRKSKM
                     ANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQDTDSNCLE
                     EEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEKIEKEQER
                     IAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQLENIQLTE
                     TFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQTKLDELT
                     QLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFELADTIGSY
                     RGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGVAIDALFI
                     DEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTEGSGQSKI
                     RYQLG"
     gene            2780..5974
                     /locus_tag="IWO52_002618"
     CDS             2780..5974
                     /locus_tag="IWO52_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0356849.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(6120..6827)
                     /locus_tag="IWO52_002619"
     CDS             complement(6120..6827)
                     /locus_tag="IWO52_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0356850.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         2645 a         1255 c         1471 g         1890 t
ORIGIN      
        1 aaaagaaata aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat
       61 gaatctcgat gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa
      121 ttttttggtt tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca
      181 atttttccaa cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt
      241 ggatcattta gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga
      301 ggaaccagca gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact
      361 cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca
      421 actagaaaat tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat
      481 attaataaac aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg
      541 atggattggg tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga
      601 aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc
      661 gatgactacg cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca
      721 aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt
      781 cgaatcctta cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga
      841 agaattgaat gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat
      901 tcttcaagaa aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca
      961 gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg
     1021 gtcgctcgca cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag
     1081 tcagctattg aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca
     1141 gcaaattgct cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc
     1201 aacggaacac cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa
     1261 agtagaacaa caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc
     1321 caaaagtgaa gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat
     1381 catggcaacg aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa
     1441 ggcatttcct gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc
     1501 tttagataga cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca
     1561 acaagcagaa aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa
     1621 taatcagctc cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac
     1681 ggatagtaac tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc
     1741 gaatcaacta gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct
     1801 tcggacacag cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa
     1861 ggaacaagaa agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga
     1921 aatctgtgca tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc
     1981 ttatcatgaa gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct
     2041 aactgagaca ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat
     2101 gatcgaaata aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa
     2161 aatctacgat gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac
     2221 gcaattacag cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga
     2281 acggtatgtg cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca
     2341 acgtttaacg aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg
     2401 gaaaaccgga ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca
     2461 taccctgtct ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga
     2521 tgtgatccag acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt
     2581 tgggtcatta gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag
     2641 tgaaggaaga atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca
     2701 acaaataaga attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata
     2761 aaaagggaga ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg
     2821 tcaatacttg caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg
     2881 gcatgcagaa gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga
     2941 gatagattac tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc
     3001 tgtggaactt tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat
     3061 gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct
     3121 aaaaacacaa aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac
     3181 gattgagact aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg
     3241 ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc
     3301 ttacacagaa aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg
     3361 ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca
     3421 tcaattatta ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct
     3481 tctcccagta gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca
     3541 tctgcagata aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt
     3601 atcattcaaa gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga
     3661 ttttttcttt atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa
     3721 atatcaacaa acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga
     3781 agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc
     3841 agagatcggc gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt
     3901 cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt
     3961 ctatggtgaa gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt
     4021 tcaggtgtta cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg
     4081 ctaccagaaa aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc
     4141 ctttttccgc acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa
     4201 attgcgagag ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac
     4261 cggttcatgg ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca
     4321 tgtcctgcaa agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct
     4381 ttcttttgat tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc
     4441 gatacgtaca gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa
     4501 tcaattggaa aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct
     4561 cattcatcca gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga
     4621 ttatcaaaag caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat
     4681 tttagccgat gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga
     4741 aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa
     4801 ctggcaggca gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa
     4861 taaaaaagaa cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc
     4921 cagcttaaga caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga
     4981 tgaggcacaa atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc
     5041 tgtacctcag cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg
     5101 gtcattgttt gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact
     5161 tgccgcagaa gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac
     5221 tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga
     5281 acaaaaaact gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga
     5341 ttctgaagca ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca
     5401 aatttgctgc gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga
     5461 acaagtaaaa gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc
     5521 tcaatttaca gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac
     5581 attttattta agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa
     5641 tgctggcgaa aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct
     5701 cacgggagca gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca
     5761 agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat
     5821 ctctgaagga acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt
     5881 ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg
     5941 catgatttta agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg
     6001 ggtaagaaag cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc
     6061 tgggattcca ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt
     6121 tatacaaaga ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg
     6181 caaattggag tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta
     6241 gcactttttg cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct
     6301 ggaagcatat gaaattcttt ctcagccagc tgaaaaatct ctttttctgg tttttggaaa
     6361 cctgttgctt gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc
     6421 ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc
     6481 ttttccttca agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt
     6541 tcttcctcgt aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct
     6601 aaatctttta gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct
     6661 gccattactt ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc
     6721 atatctgcat ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc
     6781 tgatcataca tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc
     6841 tttcggtttt cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta
     6901 aaaaagctga taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt
     6961 ttttatattt cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca
     7021 aattaatcat tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc
     7081 gaaagattta tttttttaat acagtctcaa tataactatt caaacataaa ataacgttat
     7141 ttgatttatt tgtttatagt aaattttatt tagtaatcat tgctttaaaa tactacgctt
     7201 atataacaga gaaaataagc gttataataa agaacagtat tatttaaaat gacagaaaac
     7261 a
//
LOCUS       DACEDO010000105         7178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEDO010000105 DACEDO010000000
VERSION     DACEDO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.108"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..218)
                     /locus_tag="IWO52_002620"
     CDS             complement(<1..218)
                     /locus_tag="IWO52_002620"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0356851.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKP"
     gene            complement(329..583)
                     /locus_tag="IWO52_002621"
     CDS             complement(329..583)
                     /locus_tag="IWO52_002621"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0356852.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(776..1051)
                     /locus_tag="IWO52_002622"
     CDS             complement(776..1051)
                     /locus_tag="IWO52_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ0356853.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1023..1976)
                     /locus_tag="IWO52_002623"
     CDS             complement(1023..1976)
                     /locus_tag="IWO52_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0356854.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2588..4081
                     /locus_tag="IWO52_002624"
     CDS             2588..4081
                     /locus_tag="IWO52_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ0356855.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4216..4512
                     /locus_tag="IWO52_002625"
     CDS             4216..4512
                     /locus_tag="IWO52_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0356856.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4536..5495)
                     /locus_tag="IWO52_002626"
     CDS             complement(4536..5495)
                     /locus_tag="IWO52_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0356857.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5678..6529
                     /locus_tag="IWO52_002627"
     CDS             5678..6529
                     /locus_tag="IWO52_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356858.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6550..6762
                     /locus_tag="IWO52_002628"
     CDS             6550..6762
                     /locus_tag="IWO52_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356859.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2389 a         1156 c         1295 g         2338 t
ORIGIN      
        1 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
       61 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      121 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      181 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      241 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      301 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      361 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      421 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      481 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      541 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      601 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      661 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      721 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      781 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      841 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
      901 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
      961 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1021 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1081 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1141 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1201 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1261 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1321 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1381 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1441 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1501 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1561 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1621 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1681 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1741 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1801 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1861 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     1921 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     1981 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2041 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2101 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2161 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2221 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2281 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2341 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2401 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2461 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2521 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2581 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2641 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2701 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2761 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2821 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     2881 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     2941 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3001 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3061 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3121 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3181 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3241 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3301 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3361 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3421 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3481 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3541 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3601 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3661 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3721 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3781 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3841 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     3901 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     3961 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4021 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4081 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4141 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4201 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4261 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4321 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4381 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4441 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaaa aaggaagaag
     4501 tatatccagt agtagttttt atttactctt aattgtcaaa ttaagttaga caaatcatca
     4561 atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aattcagtga ttttctagga
     4621 atattatttc gtttcgatgc gacagattga ataaaagatt catctactga attgaaatcc
     4681 atagatttca ataaaccatc tctacgtaac aatccgttag aattctcgtt taggcctctt
     4741 tgagacggcg ttcctggatc agcgaaataa atggcaatat cattggcatt actgatctgt
     4801 ttccaatttg aaaattcctt tccacaatca aaagtgatgg atttgaatag atttttcggt
     4861 acagattcaa accaatgatt taatttgttt tcaatatcaa tcgcttgtct accacacggt
     4921 ttcaatgtga tgataacttt tgataatcgc tcaactaagg taattacagc acttttatgt
     4981 ttcaaaccta cgatagtgtc accttcaagg tgaccaaact catttgagaa ttggctataa
     5041 tctttttcac gttcatgaat agagcgacgg aaagcttgtt ttccacgtct ttcttgatgt
     5101 ccatttggtt tacgcttgcc tttcatcggt aagtcagaag gattaaatag cccctttttg
     5161 aacatacgat aaatggtacg agcagaacaa cgaataggaa acgctgcacg accaacaatc
     5221 acatcgggtg tccatccttg aacaaccttt ctttgaatat attctgattg ttcctcaggt
     5281 aaaacaagcg ggcgtctacc acagtttgat ttgtttttct tatactgttg atagtactct
     5341 aaggctgatt ttccttgctt gaagaacagg tatactttat ggatcgtttg tcttgaacga
     5401 ttcaagaaat gcgcagtttt cgcaacagat tgatttattt tgaaataaga ctctatcatt
     5461 acaagttcat ccgttgtaag atgggtatag gtcatttgta ctcactcctt ataattcttt
     5521 ggacggaact attttgagtg tagcacaaat gactttttta gttgtctagc ttaattttac
     5581 aatcggcgta cttaaaaaat tggttcattg agcaacctat cgctgctaaa gtagcaaagg
     5641 aaaacgttaa aggaaagaat ttagggaaga aatcgaaatg aaatcggacg actggattga
     5701 acacacctgg cttcctctga aaagaaatag caaacgaaag aaattttggg tattgcaatt
     5761 atggccatta ggaaaacttt gtagttacgg ggcacgactt attcttgatt taaccgttta
     5821 ctcgattgga gtatttggaa cggcttatta cttgataaat gatcccaata ggattgcagc
     5881 atcagacatt tttacagtgc taatgacgtt gatttttgtc atgctattgt ctatttttac
     5941 tcaattagtc atgcaattta ccaggcaaaa attaacagca ggaaaacttt tcaaaagcct
     6001 attcgattta attgttgtaa atgcaattct agcgacttta ataaaacaaa ttgaacaatc
     6061 tggaagaaca acaaaaggag tgaaggattt tttattgacg tttattcact ttgtaaatgg
     6121 acacatgtat ttgtttgaag tgggtattat tctctttttt gtcgggtatg tatccacgaa
     6181 acttattaaa cgttcaattg aaaaagaaat tgaaattgac ctgattccga cacttcttgg
     6241 ttatgagtac aatgttgatt ttggaagata tgacgaattg atcaatacgg atatcgttca
     6301 cacgagtatc catgaatcta ctgttaaggg catcagatct attttaaaaa atggccagga
     6361 aatgaaagca atagatcgaa gaaaagagat ctacaatttt tatgaagtcg aagcaagcct
     6421 acgatatggc atcggaaaat tgtggttaga tggaagtaat attcgtcaaa acaaaccata
     6481 cattttttgg aacagtagtg atttagaact tccaatgaaa gataaataat tatggagaat
     6541 cgtttgaaaa tgattcctct atacaggatt gtgcttatag ttggcatgga gttaaaaacc
     6601 ttaaagaagg agcagttatt caaagtgaaa gaacgtcata atcagttact atgtagtaaa
     6661 acgggtacga tgaacattga ataccgaaca gaatatttag aattaacgaa tcaaactgat
     6721 tttttgttga ttcgaaagca acaagagcaa gaaaaaaatt aatggctact tatagccaat
     6781 tgattgatga atgggaacaa ttcaaggaat ctaaaattcc agaacgtctc taaaagcctt
     6841 tatttgtaaa gagcataagg aaacgtggga acacttttta aaaaaagtgt tccaataggc
     6901 tgtgcctatt acgtttctca aaaataaatg tgggaacaaa atatttaaaa ataggatagt
     6961 gaaaggatag tgaaggatag taaaaggagg gtagtaggat agcaaaaagg ataggctcct
     7021 tttcgctatc ctttttaaat tggaggaatt aatatggaag taagaataag aaatgtggat
     7081 gagaaaatcg taagagaaat tgatcgaaga tgtgacgaac ggttctgttg caaagtttta
     7141 aatctactat caaataaggt agaataatag aaaaagat
//
LOCUS       DACEDO010000106         6845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEDO010000106 DACEDO010000000
VERSION     DACEDO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.109"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(139..1830)
                     /locus_tag="IWO52_002629"
     CDS             complement(139..1830)
                     /locus_tag="IWO52_002629"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0356860.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(2250..3197)
                     /gene="arcC"
                     /locus_tag="IWO52_002630"
     CDS             complement(2250..3197)
                     /gene="arcC"
                     /locus_tag="IWO52_002630"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0356861.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(3312..4331)
                     /gene="argF"
                     /locus_tag="IWO52_002631"
     CDS             complement(3312..4331)
                     /gene="argF"
                     /locus_tag="IWO52_002631"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0356862.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(4422..5651)
                     /gene="arcA"
                     /locus_tag="IWO52_002632"
     CDS             complement(4422..5651)
                     /gene="arcA"
                     /locus_tag="IWO52_002632"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0356863.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            6112..6813
                     /locus_tag="IWO52_002633"
     CDS             6112..6813
                     /locus_tag="IWO52_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0356864.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         1930 a         1409 c         1171 g         2335 t
ORIGIN      
        1 agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac
       61 gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc agaacgctga
      121 tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca aacgaaggtc
      181 ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt tttgttcatc
      241 atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta atttgattgc
      301 atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg tttcagggta
      361 tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat agtctgctgt
      421 ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat gtgtatattg
      481 cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa agtcaaatgt
      541 attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc
      601 tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt tagcagattg
      661 gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag ttgataattt
      721 tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag accagtcaaa
      781 gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt cattccctac
      841 tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag cagctaagtc
      901 acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat tcaggtcata
      961 agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa
     1021 aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa
     1081 acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat cacggaacca
     1141 ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc aagcacgagc
     1201 ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt ataaacgaag
     1261 caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt tgtaagcgac
     1321 gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga ttggctgata
     1381 accgattttt tccataatga aaccgatcga attaccgata actgtcgaac gcaaatgtcc
     1441 cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg ttccttgatt
     1501 cccgatgttg ctatccccat aatgttcttt ttctttcact acggtttgca aaactttttt
     1561 gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt cgattttttc
     1621 aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg gcgcttttcg
     1681 gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag ctgattttgg
     1741 attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag cgtcatagac
     1801 agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt tcccttgatt
     1861 gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta gagacgagat
     1921 ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga tatcggtaga
     1981 cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc
     2041 ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg ttcaacaaat
     2101 cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt
     2161 cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat agctataaat
     2221 catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt tccttcttca
     2281 gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc gtttggtttt
     2341 gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg agcgaattgc
     2401 ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt cttttgatct
     2461 ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag caaatccgca
     2521 tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc ttctcgtcct
     2581 tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga gactgtgata
     2641 actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat gggtttaggg
     2701 gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac agtggagtca
     2761 gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg tttacttgga
     2821 tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga aaccacatct
     2881 ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca gtaaccgata
     2941 gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt ctcagaatca
     3001 gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg ggagatgatc
     3061 aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat taacgcttct
     3121 tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa agcgacaact
     3181 acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt tctagataac
     3241 aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc gtgagatgaa
     3301 tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc catgatcgct
     3361 ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt gctgcggaag
     3421 acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa
     3481 acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg atctgtgttg
     3541 tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt gatacgttct
     3601 tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac gtctgctccg
     3661 tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt ttcagcaaag
     3721 ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc aacgatacgc
     3781 atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt gtttcgacca
     3841 tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg gatagttaaa
     3901 aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca aactggaaca
     3961 ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa ttcgattccg
     4021 tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt acctaattgg
     4081 atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc agtagtaaat
     4141 gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt tccttctaga
     4201 tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa
     4261 tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc ttggaatact
     4321 gattctttca tattaaacat tctcctttag attgttttta tttaagatta aagaggaaat
     4381 cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa gtcttcacgg
     4441 acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc acttgaattg
     4501 atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata atttcggtta
     4561 taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt ccattgttct
     4621 cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc atctaatcca
     4681 agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat atcatcacca
     4741 tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc tggatggata
     4801 gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat tgcacggttg
     4861 ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa taggtggcgt
     4921 gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc tactacttta
     4981 tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg atccaaccat
     5041 acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc tgtaaacatc
     5101 gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt tcccattgat
     5161 gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa tggatagtca
     5221 tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt tgtacgaacc
     5281 ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa ctcatgaaga
     5341 ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt caagaattgt
     5401 tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta aagagtctcg
     5461 acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc aatcggaaga
     5521 tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt ttccacttct
     5581 tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa aacgtgaata
     5641 ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat atcctccttt
     5701 tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca ttaatattcg
     5761 ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt ttttattctg
     5821 tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa attaaaaaaa
     5881 taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt ttatacacaa
     5941 atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat aataacaaat
     6001 tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact ttacaaattc
     6061 tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt aatgcgtgtt
     6121 aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga acatttctcg
     6181 gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa aaaaggacag
     6241 atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa aggactcgtt
     6301 cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt gaatacaaat
     6361 aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac agctcaagcg
     6421 atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt agcacgaaaa
     6481 aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga aatacatgaa
     6541 ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca aaatttattc
     6601 attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat tccttatccg
     6661 atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc tggacaagta
     6721 atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt tactttctgt
     6781 aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc agtgaccctg
     6841 ataaa
//
LOCUS       DACEDO010000107         6829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEDO010000107 DACEDO010000000
VERSION     DACEDO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.110"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            939..1613
                     /locus_tag="IWO52_002634"
     CDS             939..1613
                     /locus_tag="IWO52_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0356865.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKSLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1610..1951
                     /locus_tag="IWO52_002635"
     CDS             1610..1951
                     /locus_tag="IWO52_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356866.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            1981..2340
                     /locus_tag="IWO52_002636"
     CDS             1981..2340
                     /locus_tag="IWO52_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0356867.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2544..2723
                     /locus_tag="IWO52_002637"
                     /pseudo
     CDS             2544..2723
                     /locus_tag="IWO52_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            2865..4946
                     /gene="cadA"
                     /locus_tag="IWO52_002638"
     CDS             2865..4946
                     /gene="cadA"
                     /locus_tag="IWO52_002638"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0356868.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            5257..6723
                     /locus_tag="IWO52_002639"
     CDS             5257..6723
                     /locus_tag="IWO52_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0356869.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2262 a         1105 c         1461 g         2001 t
ORIGIN      
        1 gtttaaggaa gaggttgtga aatcagtgcc ttgacctgag tattagagaa acaaaacgga
       61 aaaatagctt ctcatatttt accgtttttt tagctaatat cgagggtcag ctgattgaac
      121 accgtttata tcaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt
      181 caaaaaacag tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga
      241 gagcaacgtt tatttaaaaa aaagaactgc gaactctcgc agttcttttt ttaaaatacg
      301 ggataaagga agtttccagc agacaagaga agaaagcaaa agaaattgct atttgattca
      361 caaggttcta taacgaaatc gcaatcaatc aaacatatac aaagaaggtt attcaaatgg
      421 aaatctgatt tgatgatgct tctgctgttg aaataaagca gcatctagta cctgaaaaca
      481 aactaatagt aacattcgaa gatggagtag gtccgtattt tcagcatgca atgatccata
      541 tgtaagttta attcatcctt gaaatcatcg gaccagataa agttatgacc aatctatccc
      601 aagcaatcta gaagagatat tagtaaaaga acatttactg gaagtcttag atgagcctat
      661 aaatatatgt tctgatggcg tcatcagtgg tctgattgac ggaagcttag gtgtgatcga
      721 tttctctgga ccaaacaacg aaggaatcaa ggggaatccg gcacgttaag aaaaaaagta
      781 taaaaaacta gacacttgat gagcgttcat tcaattgatt gaagggacta ggctggtgtc
      841 attttttata catatatttt ctctttcgtc ttgatagggc ttatgggata atataaggaa
      901 gaaaatcatc gttttattca aaaaggagtt tttgacaaat gaaaaaacgt atattattta
      961 tcggtgacag cattacagac gcaggaagag accgagcaga agcaactgat ctaggtaaag
     1021 gttatccact tttgattgcc ggaaaactaa tctcccaata tccaacgatg gaatttgaat
     1081 gcatgaaccg tgggatagga ggagacaaaa ttgcagatct gcaagtacgc tggcaagaag
     1141 attgtttagc tttacaacct gactatgtat cgattttgat cggaatcaat gatacctggc
     1201 ataatactag cgatcaggct atattcggaa ctgagaaatc tgcgcagcaa tttgaatccg
     1261 tttatcgaaa atcactaaat gaacttcaaa cagcggaaat ctgcaacatc atcttgatgg
     1321 agccgtttgt acttcctata accgacgacc gtcatggctg gcgtaaagac ttagacccta
     1381 aaatccaaat tgttcgtaaa cttgctaagg aatatggcgc attgctggtt cctttagatg
     1441 gcatattcaa tgctttaggg attgcttatg gtttttcgaa atatacaaga gaagatgggg
     1501 tccacccaac gattggcgga catgagatca ttgcacaaag ctggatagat gcaattgccg
     1561 cagtcgattt ttttagtgga gataaaaatg agcgaaagaa aagtgagaaa tgatggaaat
     1621 acggtgggca gtaaaagaag atttacctca aatcaatcag ctgattactg atcatccaga
     1681 atcattcgta aaagcaaaaa cggcagctga cctaaatgat aagaataaac tattgctcgt
     1741 tgctgtaaca aaagaaaaac ggattatcgg atttgcgcaa gcggacgtgc aacagtcaga
     1801 agaacgggtg gatcagcgac catcaggaaa actaatccaa cacacattag taacaaaaga
     1861 aaatacggaa gaaatccaaa aaaaatttgc agaatacttg atgctggaag ctaaaaaaag
     1921 aagaataaaa tcaatttttg cccagtttta atcaaaaaca gtaatagaag gagaaataca
     1981 atgagttgtt taggaaatgt catctggttt atattcggcg gactaatcgg tggtcttagc
     2041 tggttagcag cggggatact ttggtgtatc acgatcatag gtatcccaat cggtatgcaa
     2101 tgttttaaga tcgcctccct ttcttttgcg ccttttggca aagaagtaat ttatcaccag
     2161 tctggcatgt cattgatcgt taatatcatc tggctgatcg tttcaggcct accaattgct
     2221 ctcgcacatg tcgtcagcgg catccttcta tgtatcacga tcatagggat accttttgcc
     2281 aagcaatctt tcaaacttgc tcgattggcg ttgatgccat tcggtgcaga aattgtataa
     2341 aaagagaata ataaaggtta gaaaacgcat agagtcaggt gggatacatg tatttcacct
     2401 gactctatgt gttttcctgt ataagttctc ccatgtgact tttacgtctt ttatttatat
     2461 gggattactg tttatggaaa gttgaaataa ttttcattaa agaactcttt ctatattttt
     2521 gaaagcgtag tggagcttcg aatatctggg aaggacattt tgcagggatt gaaaaaagat
     2581 attgtcctat cgaaaaatta atgaatttag acaaattgcc agcaacaggc ttcaaactta
     2641 gttgctatcc tgtcaagatc aagaaggcaa gtgcgggttg gataagagca ggggcaatga
     2701 tcgaagagaa aaagaaagaa taagccaaaa catctgtatg gaagaaattt tccgatgcag
     2761 atgtttttca ttttttcttt cggatacctt gacatatgaa taaccattca tttataatta
     2821 gttatgaaga gatattcata tgttgaggcg aggaggtcat taaaatgaaa agttacagat
     2881 tagaaggttt agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt cagaagatca
     2941 atggagtcaa ggaagcaacg gtaaacttca cttctggaaa actaacgatt gacgcagagg
     3001 aagatcattt ggctacgatt gaacaagaaa caaaaaaagt agtgaaggaa ttagaaccag
     3061 atgtcaaagt aacagaaata gataaggaaa aagtatctga acatggaaat gaaaaagaga
     3121 gaaatacgct gttccggatt ttattttccc ttgcaggtat cgctttattg ctattatttg
     3181 attttaatga accaattcgc ttgatagggt atttattgat ttatctgctg ataggatatg
     3241 atgtagtgaa gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat gagaattttc
     3301 ttatgtctgt tgctacgatt ggcgctatgt tgatcggtga atatccagaa gccgtagcag
     3361 tgatgctatt ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt cattcaagaa
     3421 aatcgattcg cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag acgacggaag
     3481 gattgatgac cgtgaaccct gaagacgtgc agatcggtca gtttgtactt gtgaagcctg
     3541 gtgaacgtgt accgttagat ggaacgatca tcgaaggaga atcattagtc gatacgtcag
     3601 cacttacagg agaatcagtc cctaaatctg tatatgttgg tgaaacagta ttaagcggat
     3661 ttatcaataa aaataaacca tttttagttc aagtagaaaa atcttatgaa aattctacta
     3721 tcagcaaatt attagaatta gtagaaaatg ccagcagtaa aaaagcacca gctgaaaatt
     3781 tcattactaa attcgcgcgc tattacacac cagtcgttgt aggattggcc gttttattag
     3841 ctgttttacc tccacttgtt gtttctggag cggcgttttc tgaatggatc tatcgagcat
     3901 tgacattttt agtgatctca tgtccatgtg cattagtgat ttccgtgcca cttagtttct
     3961 ttggcggtat cggaggcgca agtaaaatcg gtgtattagt aaaaggttca aattacttgg
     4021 agttgcttgc gcaaaccgag acagtggtat tcgataaaac cggcacatta acgaaaggtg
     4081 acttttcgat ccagacgatc gatacaaaaa tcgatccaaa gaaattcatg caatatattg
     4141 cgagtgcaga acagttttct acacatccga ttgctcaatc tgttttagaa ggctacgcag
     4201 gtccgctttt accaacaact aatatccagg aatttgcagg tgaagggatc ttagctgaag
     4261 ttgatggcaa acaagtatta gcaggtaatc acaaattgat ggaaagattt gaaatctctt
     4321 ttcctttaag tcaagaaatc ggtacattgc tttatctagc gatcgatcaa agttacagtg
     4381 gttatcttgt tattgcagat accttgaaag aagatgccgt agacgcattg gttcaattaa
     4441 aacaggctgg tgtgaagaac acagtcatgt tgacgggcga ctcgaaaaaa attgctgatc
     4501 atatcgggaa gcaagtaggc gtggacaaga tatatagcga attgcttcct gaagataaag
     4561 tgcagcggtt agaggaaatc ttacaaagaa acaataaaaa gaccgcattt gtcggagatg
     4621 gaatcaacga tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg ggtggattgg
     4681 gaagcgatgc tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa ccatcgaaga
     4741 ttgcagaagc aatccattta gcaaagaaaa cattgaaaat cgtcaaacaa aacattgtct
     4801 ttgctattgg gataaaaatt ttggtacttg ccttaggtgc ttttggtttt gcatcaatgg
     4861 gagatgcagt atttgcagat gttggggtga cagtattagc tgttttgaat gccatgcgaa
     4921 gcttgcatgt aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt cctctttttt
     4981 ttatatcctt ttcttaatga aaaatgtaaa gaaaatagat aggattataa aataactaat
     5041 gtttttaatt ttgcagaaaa ttcaaaatag cttgaaattc aataaggtca atgaaataaa
     5101 agaaaataac ttattttttt gatggaaaaa atatgaaaaa acgatgtgta ttaaataaaa
     5161 gaatacacac ataaaataac actgtcttgt aagtttgtta aaaattgaat atgattacat
     5221 ctggataaca aatttttatc ataaaggaga gaacgaatga acaacaaaca attacgctgg
     5281 tttactgttg cgttaattgc atttaacatg gtttggggca tggggaatgt agttaataac
     5341 tatgctcagc aaggagtatc agttgtcacc tcttggctat tgattttggc catttatttt
     5401 attccatacg cattgatcgt tggacaatta ggatcagcct tcaaggatag taaaggaggc
     5461 gtcagttcat gggtcgaaaa tacgacatct aataaacgac tggcatacta tgccgcttgg
     5521 acttactggg tcgtccatat tccttatttg gcacaaaagc ctcaagcaat tctgattgct
     5581 ggtggttggg cagtccaagg aaatggaaat atcgtaaata cgatgtcagt acaaatggta
     5641 gcagttattt cacttattat atttcttgcc tttttatatt tatcaacaaa aggactttct
     5701 actttgaaag tgattggcgg actggccgga acagcgatgt tcgtcatgtc gatcttattt
     5761 attcttttag ctgtaacggc accgttcatc aatccaacga tggaatttgc cacaccacat
     5821 atgaatcaag tgaaaacata tattccaaca tttgatttca gttatttcac aaccgtttcg
     5881 atgctagtat ttgcagttgg aggggcggaa aaaatatccc cttatgtcaa ctctacaaga
     5941 aatcctgcaa aggaatttcc taaagggatg atcttcttag cagcaatggt agggatttgt
     6001 gctgtattag gctcagtcgc aatgggtatg ctgtttgcta gtgataatat tcctaaagac
     6061 ttgatggcaa atggggcata cagtgctttt caaattttag gtaattacta tggtatcggt
     6121 aactcattga tgatcattta cgcattgaca aacatgattg gacaaatctc cgcattagcc
     6181 ttttcaattg atgcaccact aaaaatcttg ttagcagatg cggatccaga atttgtacca
     6241 aactggctac gtaaacgtaa tcgtaaaggt acattggtga atggttatct tctgacaggt
     6301 atactagtag gaatcattat tttgttgccg atttttggta tcaaagatat gaatgaatta
     6361 gtaaaatggt tgacgaactt gaactcagtt gtcatgccaa tgcgttacct atgggtgttc
     6421 ttggcatata tgatgttgaa taaagcttat aagaaattca attctgaata taaatttgtt
     6481 aaaaatccaa aaatcggttt tatctttggt gcttggtgct ttgccttcac tgcgtttgca
     6541 tgtatcctag gaatggtgcc aaaagtagat tatactgctg atcctaaagc atggtggttc
     6601 caattattta cgaatatcat cacaccaatc gtcttgatct tattaggtat gatcttgcca
     6661 gcaattgctc gtcgagacaa aaacaaagaa atcactgaac cagaaacagt ggttcagcct
     6721 taaaatagaa atacttgtta caagaggttg tgacagaagt ggtcagcttc aagaaataag
     6781 aaggaattta cgaaaattgc ttctcaaatt tttgtgaagt ttcggcttg
//
LOCUS       DACEDO010000108         6361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEDO010000108 DACEDO010000000
VERSION     DACEDO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.111"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(188..733)
                     /locus_tag="IWO52_002640"
     CDS             complement(188..733)
                     /locus_tag="IWO52_002640"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0356870.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(737..865)
                     /locus_tag="IWO52_002641"
     CDS             complement(737..865)
                     /locus_tag="IWO52_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0356871.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            complement(858..1949)
                     /gene="phnW"
                     /locus_tag="IWO52_002642"
     CDS             complement(858..1949)
                     /gene="phnW"
                     /locus_tag="IWO52_002642"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0356872.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(2166..2645)
                     /locus_tag="IWO52_002643"
     CDS             complement(2166..2645)
                     /locus_tag="IWO52_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0356873.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            3004..3786
                     /gene="rpsB"
                     /locus_tag="IWO52_002644"
     CDS             3004..3786
                     /gene="rpsB"
                     /locus_tag="IWO52_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0356874.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3885..4766
                     /locus_tag="IWO52_002645"
     CDS             3885..4766
                     /locus_tag="IWO52_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0356875.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4902..5624
                     /locus_tag="IWO52_002646"
     CDS             4902..5624
                     /locus_tag="IWO52_002646"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0356876.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            5627..6184
                     /gene="frr"
                     /locus_tag="IWO52_002647"
     CDS             5627..6184
                     /gene="frr"
                     /locus_tag="IWO52_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0356877.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         2128 a         1172 c         1277 g         1784 t
ORIGIN      
        1 atgaggagct atttttgctt ggctgttttt attacttgat acaggacagc tttttcacaa
       61 cctcttattt tgtataaaag tcattctgga ttcttgtcag cgtacaaaat gtattctgtt
      121 aactctttta aattgtttac acaaatatca gcgccggctt gttggaatct aagagcaaca
      181 gactgtttta aacgatcttt ttccccatct ctaatttttg gtactcatca taagaaatcc
      241 ccattagcga actaccttca attacaccta ctgtcaacac tccggcattt ttcccttcct
      301 ggatatctga gaccgtatct ccgactttga ttacttcatc aaccgacttg tttttgaagt
      361 actgcatgtt tttatggatc atataaggaa acggtcgacc atacccttcg acgtcttcaa
      421 aagttgccat gaagtctggt gaataccttt ctttttttgc ggtttctgct acgattgtca
      481 tcataggagc attaaatcca ttcgtggaac caatattgat cccagcgttt ctcaaccgtt
      541 cgatcgtttc caatgtgtcg ggtttcaagg tagaatgctt gaccaaatct ttaaacagat
      601 aatgttcaaa ttgttgatac aattcctcca cctctttttc ctgaggtagt tgtccataaa
      661 tagctatcca ttgttttttg acagaaggga gggaaagaag ctgttggata tgatcccatt
      721 ttaattttcc catcggttag cggatttctt tgtcagtcag ttggattcct ttttctagaa
      781 aagcatttct aaaagcgtgg acaggagcca tacagccaaa gtccacagtt gttcctgccc
      841 aatcaaaaat tacagtttta atcattaacg ttctccttat agtctttgat aattgctgtc
      901 agttccaaaa tatcttccgg atagatctcg ccaatatttc cgatacgaaa acattcatga
      961 tcgctgattt tcccgggata gatcacaaat ccattttgct ttaaatactg ataaaaatga
     1021 ttaaaatcaa atcgccagtc cttagggtaa agaaaagagg tgatgaatgg cccctgatat
     1081 tcttgaatga aaggctggaa ccccatctct gtcatttttt gtcgtagcac atgattattg
     1141 gcgagataac gtgaatatcg agtttcgatt cctccttctg cctctagttc cttcagcgct
     1201 tgttgaaaag ccaaaaccgt atgggtaggg gaagtgaaac gccatttgcc attctcgttt
     1261 tccatcacgc ggtactggtc atatagatca agtgacaaac ttcggctaac attttttcgt
     1321 ttttccagtt ccgctttatt agcaagtaca aaagaaaagc ctggaacacc ttgaatacat
     1381 ttgtttgagc tggaaatcaa aaaatcaatc cctagttcgg ctacacaaat cggtatccca
     1441 ccaaagcttg acatcgcgtc cactataaac aaaagatctc tttcttgtgt gacagcagca
     1501 atctcttcca aattattcaa aataccggaa gtagtctcgc tatggaccat tgctagtgcg
     1561 gagatagacg gattctcttc aatcagctgt gtaatcacat gagcttggag gatttgatgt
     1621 tccgatacct cataaacggt atagttcagt ttcagacatt cgcacatgtt gatcattcgt
     1681 tgtctatatg cgccgtttga acaaatcagt acagtatctt ctggcctgat cacagaactt
     1741 aataccgatt ctaccccgaa ggagctgcta ccttgcatta agaccgttgt gtatattcct
     1801 tccgatactt gagcaagatc caataatttt tttcttatcc attgtgtaat ttctttgtat
     1861 tcttgatccc aagtacaatg atcgatcagc atagcatttt tcactgtttc agaagtcgtc
     1921 aatggtccag aagttaataa tttatacata tgatttcctt tcatttcttt tttgaaatac
     1981 acaaagagca agaagctgct cttgttaaca tccaatataa atgatgatta ttgtgattca
     2041 atcaaggtgt tgaaaaattt gaataaaaaa tgatgtattt ttgtaaagag tgtaaaggtt
     2101 tattcaataa ggaaaatatt gaaaataaaa aaacacgcat tccatattag gaatacgtgc
     2161 tctgattaaa aaagtgatac ttcagttgga tggtgaagaa attcttctac tcgttttgcg
     2221 tcctcctcat tttccgagat gaccatgatc gtatcgaatc cagcaattgt actgacaata
     2281 gagggaggtt tgatctcatc taaaacagat gcaaataaat gagcattgtc tggcaaggta
     2341 ttgacgatcg tcaaaaagtg gactcgttcg actttcaata caatgtcttc aatcatttga
     2401 accagccgat cctcttcctc agtcctagtc tcaggagttc ctttttcgcg aaacaatacg
     2461 aagcgggaaa cgccattttc atcaggtgcc ttcacgattt ttagatcacg gatatctcga
     2521 gaaatcgttg cctgcgtggc ggtaacgcca taatgcccaa gtttgtttaa tagttcttct
     2581 tgcgtacgga tcgggaattc ttcaatgact tgtttaatca gcaaatgtct ttgagctttg
     2641 cgcattttct ttctccttta cgaaagattt cacatgtatc ataacaaaaa aaagaaggat
     2701 tgcccaataa tattcgtctt ttatacctaa aattatttta tggaaaaagg ataataaaga
     2761 aacaaagaga aatctactgt ttttcttgaa ttctttttat ctttcaggta aactattgaa
     2821 tgttgagcag aaaagtatat ttgctcttgc gatacggatc ttttgtctgt ataatagctg
     2881 agggtgcttt atgcatcagc aaatccacat tcttattgga ttgtcggtag gtgcttgaaa
     2941 aagtttgccg gcaaggtaga agataagaag aggaaaaata aaaccaaatc ggaggaaaca
     3001 aacatggcag taatttctat gaaacaatta ctagaagccg gcgtacactt tggtcaccaa
     3061 actcgtcgct ggaatccaaa aatgaagaaa tatatcttca cagaaagaaa cggaatctac
     3121 atcattgact tgcaaaaaac agttaaatta gtagatgcag cttatgatta catgaagagc
     3181 gttgctgaag aaggcggcgt agcattattc gtaggtacaa aaaaacaagc acaagaagca
     3241 atcaaagaag aagcaactcg tgcaggacaa tacttcgtta accatcgttg gttaggcgga
     3301 acattgacaa actgggatac tatccaaaaa cgtatcagcc gtttgaaaca aatcaatgca
     3361 atggaagaag atggaacttt tgaagttcta cctaaaaaag aagttgctgg tttgaacaaa
     3421 gaacgtgaac gtcttgaaaa attcttaggc ggtatcgctg atatgccaag aatcccagat
     3481 gtaatgtaca tcgttgaccc acgtaaagaa cgtattgctg ttcaagaagc acacaaatta
     3541 aatatcccaa tcgtagctat ggttgataca aactgcgatc cagatgaaat cgacgtagta
     3601 atcccttcaa acgacgatgc aattcgtgcc gttaaattga ttacttcaaa aatggctgat
     3661 gctttcatcg aaggaaacca aggtgaagat caagtagttg aagaagattt cacagaagaa
     3721 aacacagcaa catcaatcga agaaatcgta gatgttgttg aaggcgacaa ttcttcagca
     3781 gaataatttt gaatgatgga gctgtttcaa aggctgggcg acaagcctca ctctcctttg
     3841 aagcagcttt ttttaaaaag aaaaacacac aggaggagtt aaacatggca gacgttacag
     3901 ctaaaatggt aaaagaacta cgcgaaatga ctggcgtagg aatgatggat gcgaaaaaag
     3961 cattagttga agtagaaggc gacatggaaa aagcagttga tcttttacgt gaaaaaggaa
     4021 tggcaaaggc tgcgaagaaa aatgaccgca ttgcagctga aggattggct tcagttgctg
     4081 tgaaaggtaa cactgcagca atcgttgaag taaactcaga aacagacttt gtttctaaaa
     4141 acgaaatgtt ccaagattta gtaaaagaaa ttgcagaact tgttgctgaa aacaaaccag
     4201 cagacatgga agctgcaatg aaaatcaaaa cagataaagg tacaattgaa tctgacttga
     4261 tcgaagcaac tcaagttatc ggagaaaaaa tcagcttccg tcgctttgaa gttgtagaaa
     4321 aagacgacaa tgctgcattt ggtggatact tgcatatggg cggacgtatt gccgtattga
     4381 ctgttctaga tggaacaact gacgaaacag ttgcaagaga cgtagctatg cacgtagcag
     4441 ctatcaatcc tcgttatgta aacgaatcac aaattccaga agctgaatta gaacatgaaa
     4501 aaactgttct tacagaacaa gcattgaacg aaggcaaacc agcgaatatc gttgaaaaaa
     4561 tggtagaagg acgcttgaag aaattcaaag cagaaatcgc tttggtggac caaccattcg
     4621 ttaaagatcc tgatatgact gttgaaaaat acgttgcatc aaaaggcgca actgttaaaa
     4681 cattcgtacg tttcgaagtt ggcgaaggta tcgaaaaacg cgaagataac tttgtagaag
     4741 aagttatgag ccaagtgaaa aaataattct gttgaataac agacgggaac gcacggtttg
     4801 tgcgctccct ttttttaagc aaaacaagaa aaaccagtct tagagtgtcg aaatcatcta
     4861 agtatggtaa aatggcaata gaaaatattg gaggaatgaa catggtcaca ccgaaatatc
     4921 agcgtgtagt cttaaaattg agtggagaag ctttagctgg ggacgcagga tttggaatca
     4981 aaccaccagt aatcaaagaa atcatccaag aaatcaaaga agttcacgag ctgggcgttg
     5041 aaatggcaat cgttgtgggc ggcggaaata tctggcgcgg acaaatcgga gcacaaatgg
     5101 gcatggaacg tgctcaagct gattacatgg gtatgttagc tacagttatg aatgccttag
     5161 ctttacagga tacgttggaa aacgtgggtg taccgacacg cgtgcaaact tcgattgaaa
     5221 tgcgtcaaat cgcagagcca tatattcgcc gtaaagcaga acgtcattta gaaaaaggac
     5281 ggatcgttat ctttgcaggc ggtaccggca acccgtactt ctcgacagac acaacagcag
     5341 cattacgcgc ggcagaaatc ggcgcagatg ttatcctgat ggctaaaaac aatgtagacg
     5401 gtgtttattc tgctgatccg aaagtagatg caaatgcagt aaaatttgaa gaactgactc
     5461 atctggaagt tattgccaaa ggattacaag tcatggattc aactgcaagt tcactaagca
     5521 tggataatga tattccactg cttgtcttca atttaaatga acacggaaat attcgccgtg
     5581 caatcttagg cgaaaatatc ggaacaactg taagggggaa ataaaaatgg cagatgcaat
     5641 tatgacagaa gcaaaagaaa aaatgaaaaa agcagcacaa aatcttcaac gtgaacttgg
     5701 gcaaatccgc gcaggccgtg cgaacgctag ccttttggac cgtattactg tgaactacta
     5761 cggtgcgcca acaccattga accaaatggc atcaatccaa attccagaag cacgtgtttt
     5821 aatgatcaca ccttttgaca agagcattct tcaagatgtt gaaaaagcta ttatggcaag
     5881 tgatatcgga atcagtccaa ccaatgatgg aaatgtcatt cgcttagtta tcccgcaact
     5941 gacagaagaa cgcagaaaag aattagctaa agatgtgaaa aaagaagcag aaaatgcaaa
     6001 aatcgcgatt cgtaacatcc gtcgtgatgc gatcgatgaa tataaaaaac aacaaaaaaa
     6061 tggtgatatt accgaagatg atttgcgcgg attagaaaaa gacgtacaaa cattgacaga
     6121 taacagcatc aaagaagtag ataagatcgc tgctgataaa gaaaaagaat tattggaagt
     6181 ttaataaaat cataaaaaca gaggggcgga tcattccgct tcctctgttt ttatttatat
     6241 ttcttttatt taaaaattaa aaaaagaaaa aattattcag tttttaaata aaaaaacaat
     6301 gatttcatag gaaaaacagt tagatattgc tataatactt aagatagtgg gaatctttta
     6361 a
//
LOCUS       DACEDO010000109         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEDO010000109 DACEDO010000000
VERSION     DACEDO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.113"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWO52_002648"
     CDS             725..811
                     /locus_tag="IWO52_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0356878.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWO52_002649"
     CDS             complement(932..1621)
                     /locus_tag="IWO52_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356879.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="IWO52_002650"
     CDS             complement(1866..2780)
                     /locus_tag="IWO52_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0356880.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWO52_002651"
     CDS             complement(2762..3142)
                     /locus_tag="IWO52_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0356881.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWO52_002652"
     CDS             complement(3592..3879)
                     /locus_tag="IWO52_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0356882.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWO52_002653"
     CDS             complement(3899..4123)
                     /locus_tag="IWO52_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ0356883.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWO52_002654"
     CDS             complement(4763..5296)
                     /locus_tag="IWO52_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0356884.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWO52_002655"
     CDS             complement(5317..6159)
                     /locus_tag="IWO52_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0356885.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACEDO010000110         5870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEDO010000110 DACEDO010000000
VERSION     DACEDO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.114"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            329..784
                     /gene="tnpA"
                     /locus_tag="IWO52_002656"
     CDS             329..784
                     /gene="tnpA"
                     /locus_tag="IWO52_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0356886.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1049..1147
                     /locus_tag="IWO52_002657"
                     /pseudo
     CDS             1049..1147
                     /locus_tag="IWO52_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1370..1600)
                     /locus_tag="IWO52_002658"
     CDS             complement(1370..1600)
                     /locus_tag="IWO52_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0356887.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1830..2648)
                     /locus_tag="IWO52_002659"
     CDS             complement(1830..2648)
                     /locus_tag="IWO52_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0356888.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            2809..3498
                     /locus_tag="IWO52_002660"
     CDS             2809..3498
                     /locus_tag="IWO52_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0356889.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            3512..5014
                     /locus_tag="IWO52_002661"
     CDS             3512..5014
                     /locus_tag="IWO52_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0356890.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5027..5503
                     /locus_tag="IWO52_002662"
     CDS             5027..5503
                     /locus_tag="IWO52_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0356891.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(5600..5710)
                     /locus_tag="IWO52_002663"
                     /pseudo
     CDS             complement(5600..5710)
                     /locus_tag="IWO52_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1997 a          867 c         1056 g         1950 t
ORIGIN      
        1 ttctttgcca gataatcact tccgccttta ccatccaagt aatcatgaac gatctttaat
       61 ttaagttcaa aagtatactt agccataaaa aataccgacc tccaaaagtt agattttttg
      121 gtctaacttt tgggggtcgg tacaaaactt aaaagtttta gaccctttta ttttcttaat
      181 gacttcagtc agttttgtgc gtaaagcgtg cgaaacaaga aaaccgcgcg tttctacgcg
      241 tggaagaagt atctttagcg ataaaaactc ctttcgatat actagtgagt ggctaccaac
      301 cgctcatcag atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcatatac
      361 tcgttggaat tgtaagtatc atattatttt tacaccaaaa tatcaacgaa aagtaatcta
      421 tgggcaatta cgaaaagatc ttggaaaaat acttaggaag ttgtgcgaaa tgaaagatgt
      481 ggaaatcata gaagcacatg caatgctaga tcatattcac atgcttgttc gagtaccacc
      541 aaaaatatgt atatccagtt ttatggggta tctgaaaggt cgtagtgcgg tactaatcca
      601 cgagcagcac gcaaatctca aatataaata cggaaacaaa tccttttggt cgaagagata
      661 ctatgtgagt acagtcggat tgaatcaaaa gacaaatgaa aaatacatcc gtgaacaaga
      721 agctgatgat cgcattcgat atattataag taaaagagag tacgtagaac catttaaaaa
      781 ataaagagaa gtaagtgagt gaagatgagc agttggcggt cttttaaaaa tcccctttta
      841 aggggcagcg agtgtcgacc ccttctaggg ggattaaaaa tacacccgtc ctcacgggtg
      901 tatgggttac tgatagactt gattttaact ggtgtcagaa acttatgtcc agaaagggtg
      961 atgaaatttt ggcaatttac ggctattcaa gaattagcac aagcaaccaa gattacaaaa
     1021 cacaaattca aacattagaa aatgctggcg cagaaataat cttcctagaa aatcactgga
     1081 ttatcgaaca cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt
     1141 aatttgacaa ttaagagtat atttacttca gatggtaagg caacaaaata aaatacaata
     1201 ggcagatgaa ctaataaata aaatctgtat taaatacaga taaattaata taaaaatcaa
     1261 aaggatcata aacttttaac aagtgatgaa atgggtggaa attgattctt atagacagtt
     1321 tgtctcataa cattgaagta ttttgcaatt ttagcattga aacacttttt taatcttcaa
     1381 cgttgttctt ataaacaaga tgattgggaa tcttttgaat taacactgta tagcttagaa
     1441 caccctttat agaaaacatt cgcttagcaa tcttaataat tccttgtgcc cgatgtcgtt
     1501 tagattcagt aggttcctgc tcatcgatca tggaaaaaat ttgaaggatc agatccttaa
     1561 taaatttatt taagagagga tgatccattg cttcatccat actcgataaa ctagtgataa
     1621 tgagctacac gtttttcttt caagttgatc aatagcttcc acgataaaaa tatctttaga
     1681 taaaatcaag aaccgtagga actacaggga tatttggtca ataagagcca ccgctattag
     1741 taaaaaataa gctatcaagt atgctctcat cccaagaatt gcctacttca atcgttaaaa
     1801 accgttaggt ttagctaagt gggggtagtt cactcaatac ttttatcaat aatagagcgc
     1861 aaaacttttt ctcttaattc aactgaatca atattattgt tttgttcata accatgaact
     1921 aatagatcac aaatataaag ttgtgatatt tttccagcta aagatccacc attaagaaat
     1981 tcttcaatgg ctgtttgtaa aactaagtct gcagactttc ctataggtga atgaatatag
     2041 ttagttatag caataatttt tgcgccattt tttttagcta ttttcaatga atcataagta
     2101 tctttcgttt tcccagataa agaaaaaatg attactaaat cattaactgt taataaagaa
     2161 gcgacttggg cttgaaaatg agggtctagt acagcttttg cttgaactcc tacgcgaaga
     2221 aacatatttt ctaaatcaag actagtattt ccactagatc ccactccaaa tatatatatg
     2281 ttttttgatt gagtgatcat tttaatggct tcattcaatt tttcttcgtc taaaagccta
     2341 atagtggaat agagagcttc tgttaaacta ttagcaattt catcatagta ctttacacta
     2401 gtcggatgtt cttttttttg actaaagtct tcctttgcaa tttcaatttt taaatcagaa
     2461 aaaccagaaa aaccaatttt ttgacaaaag cgaataatag tagcatcgcc aacttttgtg
     2521 tttttcttaa tatcactcat cgtactataa ataatagttt tcccagaatt taaaataaaa
     2581 tcagcgactt ttttttcaga cttcgttaaa aatggataat tttctttaat taaatagata
     2641 taactcatac aaactcctct acaatttcaa cttttctcca ttatactcca aaataataaa
     2701 aaaaactcct aaatactcca tttttatctt tacttttatg aaagcggatg ctatattgtt
     2761 tttggagttg ttatccaaaa ataataaaaa attggagtgt ttaggataat ggataaaaag
     2821 aggtttttag atcttatttc aaaaggtttg attgtttcct gtcaagcatt acctggtgaa
     2881 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
     2941 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
     3001 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
     3061 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
     3121 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
     3181 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
     3241 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
     3301 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
     3361 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
     3421 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
     3481 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
     3541 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
     3601 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
     3661 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
     3721 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc
     3781 tggtggagta gcttatctag tatatactgc aactattagt gggtttcttg aacttttttc
     3841 agcaaaagat gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt
     3901 tgcattttta cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg
     3961 aggatcaaga tttattccga ttgtttctag tttagctgca atcattattg gttctttctt
     4021 ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca
     4081 aatgggtagt ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg
     4141 actacaccac accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt
     4201 agctggatca acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa
     4261 ccacactggt ctgtttactt atggtactag attttttgca ggaagatttg caacaatgat
     4321 gtttggatta ccagcagcat gttatgcaat gtatcgtgca attcctaaga aaaatcgtaa
     4381 aaagaatggt gggctttatt ttagcggggc ccttacatca tttttaactg gaattactga
     4441 accagttgaa tatatgtttt tatttgttgc tccttggctt tatgtgattc atgcattctt
     4501 agatggatta tctttttatt ttgctgatat cttaaatatt cgtgtaggga attccttttc
     4561 aggtggtttg atcgactttt tattattcgg tgtacttcaa gggaatgaca aaactaattg
     4621 gttaaaatta attccttttg gtattgcatg ggcatttata tatttctttg tttttacatt
     4681 ctttatcaaa aaatttaagg tagctattcc tggaatggaa gaagaatcag aagttcattc
     4741 tgaaaaagta ataaacaaaa atacatcatt acatgaagag tctcttatca ttattgaagc
     4801 tcttggtgga aaagaaaata ttgagtctgt tgaagcttgt gcaactcgtt tgcgtgttgc
     4861 tgttacgaat ggggagatta ttcaaaaaca aacaataaaa gacactggtg caactgctgt
     4921 cttcgaagtg aagggtggca tccaagctgt ttttggaggg aaagctgact tattaagtca
     4981 agaaatcaat caaatattag gaaaagataa ttaggaggtt tttaatatgt ttgaaatttt
     5041 caaaaagaaa aaacaagaaa ctttttatgc accttgttcc ggaaaaatca tttccatcac
     5101 agaagtaaat gatcctgtct tttcacaaaa attactaggt gatggttttg ccgtcatacc
     5161 tgatggttca aaaatttatg ctccattggc tggtcgagta acaaatatat ttcctactaa
     5221 acatgctatt aatataaaaa gcaactcagg cattgattat ttgattcata ttggaattga
     5281 tacagttgaa ttacaaggag agcctttttc gatttgtgtt caagaaaatg atgaggttac
     5341 tcctgaaacc tcgttggttg atgtggattt tgacaaaata ataaaatctg gtaaagatac
     5401 ttctgttata gttgtttttc cggagaaaaa tcagttcaat gatttaataa tagataatgg
     5461 tcgaattgct catgggaatt catgtggaaa aatcactttt taaaaaagtg tatataaaaa
     5521 gctataaaat aagctaaata ggatatttgt caaatgaagt tggtgaacag aaagaactgt
     5581 tttctttctg ttcatttttt tattttatta gtaatgattc cccatgccaa atcatgatcc
     5641 gtaatttaaa gttataaaag cttcgaaatc cattggcatt tcgttggaat accttgatat
     5701 gattattcaa atataaaata tagagagaag agagagaggg aagatattta taaagggcaa
     5761 agttatgttt actcaactta ggaaatagaa atagatcaat ttatattctc ttgtcaatcg
     5821 aatatgaacc ttagcactat ataccaaata tagtaaaatg ctgaaagatg
//
LOCUS       DACEDO010000111         5862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEDO010000111 DACEDO010000000
VERSION     DACEDO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.115"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWO52_002664"
     CDS             complement(99..1229)
                     /locus_tag="IWO52_002664"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0356892.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWO52_002665"
     CDS             complement(1878..3080)
                     /locus_tag="IWO52_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0356893.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWO52_002666"
     CDS             complement(3140..3343)
                     /locus_tag="IWO52_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0356894.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWO52_002667"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWO52_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWO52_002668"
     CDS             4880..5692
                     /locus_tag="IWO52_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0356895.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1735 a         1209 c          938 g         1980 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa tt
//
LOCUS       DACEDO010000112         5774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEDO010000112 DACEDO010000000
VERSION     DACEDO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.116"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(120..872)
                     /locus_tag="IWO52_002669"
     CDS             complement(120..872)
                     /locus_tag="IWO52_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0356896.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1236..3902)
                     /locus_tag="IWO52_002670"
     CDS             complement(1236..3902)
                     /locus_tag="IWO52_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0356897.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4243..5694
                     /gene="cls"
                     /locus_tag="IWO52_002671"
     CDS             4243..5694
                     /gene="cls"
                     /locus_tag="IWO52_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0356898.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1683 a         1212 c         1007 g         1872 t
ORIGIN      
        1 gtaagataac aaaaaatctg agccagaatg aggttcattc tgacccagat ttgattttgt
       61 acaacccatc agtccttatt gacaaagagc caatataata aaatcataaa aagaaccctt
      121 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
      181 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
      241 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
      301 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
      361 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
      421 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
      481 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
      541 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
      601 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
      661 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
      721 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
      781 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
      841 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
      901 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
      961 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     1021 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     1081 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     1141 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     1201 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     1261 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     1321 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     1381 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     1441 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     1501 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     1561 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     1621 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     1681 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     1741 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     1801 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     1861 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     1921 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     1981 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     2041 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     2101 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     2161 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     2221 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     2281 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     2341 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     2401 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     2461 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     2521 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     2581 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     2641 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     2701 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     2761 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     2821 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     2881 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     2941 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     3001 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     3061 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     3121 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     3181 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     3241 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     3301 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     3361 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     3421 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     3481 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     3541 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     3601 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     3661 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     3721 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     3781 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     3841 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     3901 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     3961 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     4021 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     4081 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     4141 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     4201 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     4261 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     4321 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     4381 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     4441 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     4501 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     4561 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     4621 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     4681 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     4741 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     4801 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     4861 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     4921 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     4981 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     5041 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     5101 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     5161 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     5221 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     5281 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     5341 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     5401 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     5461 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     5521 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     5581 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     5641 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     5701 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     5761 tcccttcttc cgaa
//
LOCUS       DACEDO010000113         5408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEDO010000113 DACEDO010000000
VERSION     DACEDO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.117"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..138)
                     /locus_tag="IWO52_002672"
     CDS             complement(<1..138)
                     /locus_tag="IWO52_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0356899.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
     gene            354..965
                     /locus_tag="IWO52_002673"
     CDS             354..965
                     /locus_tag="IWO52_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0356900.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            1038..1967
                     /locus_tag="IWO52_002674"
     CDS             1038..1967
                     /locus_tag="IWO52_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0356901.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            2125..2862
                     /locus_tag="IWO52_002675"
     CDS             2125..2862
                     /locus_tag="IWO52_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ0356902.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2910..4088)
                     /locus_tag="IWO52_002676"
     CDS             complement(2910..4088)
                     /locus_tag="IWO52_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0356903.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            4111..4194
                     /locus_tag="IWO52_002677"
                     /pseudo
     CDS             4111..4194
                     /locus_tag="IWO52_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4315..4716
                     /locus_tag="IWO52_002678"
     CDS             4315..4716
                     /locus_tag="IWO52_002678"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356904.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4923..>5408)
                     /locus_tag="IWO52_002679"
     CDS             complement(4923..>5408)
                     /locus_tag="IWO52_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0356905.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1697 a          909 c         1039 g         1763 t
ORIGIN      
        1 cttcagttga tcagtatttc ttcggttttt aatcttatct ggtacacaat tgtggtatat
       61 gttagaaaca gttctcataa tttccttgaa tgttataaaa gtatctgtat aatgttcagt
      121 atattttgat gaacgcatat gtagtcctca tttcttggga gttttgagac tactatatgc
      181 gtttttttgt tgatttacca acatgtttag acatttccct ctaggaaaaa attaactagc
      241 accacgggtt atgatataat aaaatgtatt ataggcattt ttttacaaga aatagaagct
      301 tgaagtttat atgaagttat aaaatttaat ttatattgga aggaggaaaa taaatgtatt
      361 ttatactacc tacactttat agccttttat gtgtaatggc accatgtatt atttatatgg
      421 tatttttttg caaaaaaaat accctgaaac acaatatcct gattttgatt tttatgctgt
      481 actgttggaa agtttttgaa ttgactggta caggtggact aacagatatt ttttatagac
      541 ctgatacagg taggaaattt tctctagtac agggcagtgt taatcttgta ccattcagtg
      601 gactaaacat tagctttata ttaaatatag taatgtgtgt gcccttagga ttcttactac
      661 caatcatatg gaaaagttat aggaaaatag ttcctgtttt cttggctggg gctctatttt
      721 cactaattat tgaagtctca caaattataa ctacaagagc aacagatatt gatgatttaa
      781 tagcaaatac ctttggtgcg gttataggct ttatactgtg ggctgtttta agtagagtgg
      841 tgtccttgaa ggacaagtca tctacagtag attatttaga accttatatt tatatattga
      901 ttggttttac cggaaaattc ttactgtatt ttcctttctg gtttgctttt gaagtattat
      961 cctgataatt ccatagaaaa taatgagttt gagttgagaa agaatattct tgtacacaac
     1021 tctggaaagg aggaagtatg aaaagattct taaaatgggg gcttgttctt ttagcgtctt
     1081 tgattgtgat tctaatgtgt acagcggttg ttatttctta tgtgaatcgg ccgtatgcaa
     1141 gagctgttta tgataggatt aaacgcattc ttgacaaaga tttttatgac catctatatc
     1201 taggttcttc atttgaaata ataaaccttg atacaatgga tattcatagc tatatgaaaa
     1261 aagaccaaga agaattaccc gcctcattga gcaaattatt tacgattgat tatgcgctta
     1321 gtgtcctaag tttagatgaa cagattacaa ttagtcctac tgcacttgac atgcagaaag
     1381 ctgactcatc tactgcacat cttcaagaag gagatatttt atctgtttat gacattatct
     1441 gtgctatggt cttaccctca ggaaatgatg cagcttatag tttggcaatt tatacaggtg
     1501 gaaaacttga tcctgatgca aagaatgatg aagaacgcta tcaaagcttc atgaaaaact
     1561 tagatcgtta tctaagtgag caaggctata aacatactca ttttactgac gctgctggct
     1621 atgactccaa tgctacaaca tcagcggaag atttgacaaa agtagctgca aagctactaa
     1681 agaatgataa atttgcagaa atagttgcca aggaaactta tagagcaaaa tcaattaagg
     1741 gaaatgttta taactggaat actacgaatc aatttatgca acaagataat agatacttta
     1801 ctgaaggtgt aaaaggtgta aaaactggat cgtttgatgg gagatacaat ctatgtgtgt
     1861 attatacatc tagttctgat aaccactatt tacttgtaag ttttggttca gagagtgata
     1921 gtaggcgtta tgtagatgtt caaaatctat tgcataatct gccttgaaat ttcgtgcctt
     1981 taagcgcgta tctaaagttt ctttgaaaag tttgagaatg aagaaagtga gcagtcgaaa
     2041 aacggttgct cacttttttg ccctaaaatc gaaaaaatat aaaaaagtcc tttacaacac
     2101 gtctcaggaa aaactgctaa gtcaattcta atatcttgtg gagcaagact tgcacctttt
     2161 gatataagag agcttagaga actaatgagc gaagatgaac tagaactcga taaaattgga
     2221 gatagaaaaa ctgccttgtt tgtaataata tcagatacag atgatacatt taattttgta
     2281 gtttcaataa tgtattctca attatttaat ctactatgtg ataaggcaga tgatgtgtat
     2341 ggtggaagac ttccagttca tgttaggtgt ctacttgatg agtttgcaaa tatcggtttg
     2401 attcctaaat ttgaaaaact aatagcgaca attcgttcaa gagaaatatc ggcaagtata
     2461 atactgcaag cacaatctca attaaaagca atttataaag accatgcaga tacaatagtt
     2521 ggtaactgtg actctacact ttttctagga ggaaaagaaa aaacaacagt aaaagaatta
     2581 tcagaaacct taggaaaaga aaccatagac ctatataaca catcagaaac aagatcaaat
     2641 caaaaatctt ttggtctaaa ttatcaaaag actggcaagg aacttatgag tcaagatgaa
     2701 ataactgtaa tggatggtgg taagtgtatt taccagctaa gaggagcaag accttttcta
     2761 tctgataaat ttgatattac aaagcataag aattacaaat tgttggaaga ttatgataag
     2821 aaaaacttgt ttgatgtaga agagtattta aaacaaagat agcgtaaaat attttgtgta
     2881 aataactcgt ccttctgcaa aataatgggt tactcagtaa acattgaagc taatgtatcc
     2941 gtcacttgtt ggaagccttt atggcttcta tttaagaatt tttgattgta tgtatcaaag
     3001 atggatacta ggaaacgctc tagtgattct tcattttgaa actgctcttt tctgtggctg
     3061 tatttcttga tttgcttatt aaaggattca atcaagttcg tagagtaaat agtcttgcgt
     3121 atggctaatg gaaaatcata gaaagtgagc aggtaatcgt tcgaaaggat cgattccacc
     3181 atttttggat aactggtttt ccatttttcc gcaaaagcac cacgtgcttc caatgccgcc
     3241 tttttagatg acgcttgata caccatttta aaatcatcac agacttcctt acgatcatcg
     3301 acacgcactt tgtgactgat attacgggaa acgtgcacac agcaatgttg aaaacgggct
     3361 ttggggtaga accgctgcac ggcccctgcc atccccttta agccatccgt gatgaaaagg
     3421 aggacgtttt tcacgccacg ttcctggagg tctattaaaa tctcttccca aatcgtgata
     3481 gattcagttg gtgcaatcgc atagctcaac acttcctttg aaccgtccgg gcgaatgcca
     3541 actgcgatat gaatggcttc cttggcgacg gttttccgct ttaaagggat ataggttgcg
     3601 tccatataaa tagcggcata acggtcatga agctcacgtt ccttaaatga agtgacttct
     3661 tccgtaaatg atttagtcat attggacatg gtttggggcg tgtagtgatg cccatacatt
     3721 ttctcaatca agtctgcgat ttccgacatg gtaatacctt ttcggaagag gtgaatgacg
     3781 gtctcctcta aggtatcatt cgtccgtcta taagcaggaa cagtctgttg cttgaactcg
     3841 ccgttgcggt cgcgcggaat ctggagatga agttccccgt actcggtctt gaccgtacgg
     3901 tcataggagc cgttacgaga attacccgtg ttaaaaccaa tgcgatcgta cttttcgtaa
     3961 tcgaggaaag ccgttaactc cgttttgaga agcgtattga cagctttttc caaatggaca
     4021 cggaataatt cattcaaatc gcctttattc gctagagtct ttagaatttc tgtagtaaaa
     4081 tcgttcatat ggaagtcctc ttttctgtga atgtgttgtg gtaactctat tctactgaag
     4141 ggacttcctt ttttctattt acacaaaata ttttacactc tcggattgaa agaacgcggt
     4201 ctttcaggag taaagatggt catctcggat gcgcatatag ggctcacaaa agctgtgaaa
     4261 gaataccttc tttgtgcgag ctagcagaga tgccaagcac atctccttcg caacatttcc
     4321 gacaaattct ccaagaaaat ctcaagcgat atcaaagaag actttaaagc catcttcaaa
     4381 acggcaagca tcgagctggc tggagaacgt aaacaactta ttatggataa atatggggaa
     4441 gacaagaaag tcgcaactgc ctgtaaaata ttggatgagg gcttcgaaga tgccatgcaa
     4501 gtaatggttt taccagaaaa tatccgtcaa cgattgagaa caacgaatgt tcttgaaaga
     4561 ctaaatgaag aagtacgccg tcgtgaacgt gttgtccgaa ttttcccaaa ttgtgattct
     4621 gttattcgta tcatcggtgc tttattgatg gaaaaggatg acgaatggac ttctgcacct
     4681 agaaaatatc ttgaatttga tcgtaacgag atttagcaca ttaagccgcc aatagtataa
     4741 tatactggtg agaagtatca agagacgctt cgctttttgc tcttgacact tcttaccagc
     4801 atattggacc gtagctaggc tcaacaaggc aaaacatcgt actttgagat ttccttttta
     4861 cacaagattt tggacttgac acagccccta tggttcttac cttgtttatg cttatttaaa
     4921 tcttacttta ttacagtact ttcaaacttc gttttgtaag ccgggtattt tgatatttca
     4981 taattatgtc cctctttttg aatatattct ttgatatctg cttgttcatc tacactttct
     5041 ttgcgtaaaa tacgttcgat cattgagcag cagtgcatgt aaagcaagaa caattgattg
     5101 tttgaaaatt cgatccccat tagtttttcg agttctttga cggccttttg taccaatctc
     5161 aaaaccttca ttgcatcaag gatcgttaaa ttttctacga tcttgttaat cgagaaatta
     5221 aagtattgag cacaattata ggcgataaat ggagcatttg attctatcac ctgttgttcc
     5281 ttggcgtatt cataaatttt ttctgccaaa aaggtttttc ctgaaccgct tacacccatc
     5341 aataaaatcg gtaatccgtt atgaggatac aagacagccg tcttgatttg atcgatcgct
     5401 tcctttaa
//
LOCUS       DACEDO010000114         5341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEDO010000114 DACEDO010000000
VERSION     DACEDO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.118"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IWO52_002680"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IWO52_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IWO52_002681"
     CDS             complement(546..740)
                     /locus_tag="IWO52_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356906.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="IWO52_002682"
     CDS             2092..3132
                     /locus_tag="IWO52_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0356907.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="IWO52_002683"
     CDS             3956..4288
                     /locus_tag="IWO52_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0356908.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="IWO52_002684"
     CDS             4907..5248
                     /locus_tag="IWO52_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0356909.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtgata tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gcaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttt tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattaa
     4861 tataaagcat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa ggggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 t
//
LOCUS       DACEDO010000115         5298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEDO010000115 DACEDO010000000
VERSION     DACEDO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.119"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(899..4048)
                     /locus_tag="IWO52_002685"
     CDS             complement(899..4048)
                     /locus_tag="IWO52_002685"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ0356910.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(4061..5278)
                     /locus_tag="IWO52_002686"
     CDS             complement(4061..5278)
                     /locus_tag="IWO52_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0356911.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
BASE COUNT         1523 a         1071 c          847 g         1857 t
ORIGIN      
        1 atgttatcag gttcctagcc tttatttaag tattacccca cagacccttc catatagatt
       61 tttaaattac acactagttt aaactaaaac aaacaaggaa aaacttacca tttaatcaaa
      121 aaattagctc atatgaatat attagaatgt aaattagact ggtcaaaatc tagtcaaaaa
      181 agaggacgaa tacaggtagt cctctttttt gttattttga tattctaaat cattttttat
      241 tggattattc ccttgttcga tatagaccca aattgatcta tcaataatta tcaaactgag
      301 tcatatagag acaaaaattt caaaaatgac ctgttaaatg gtcatcgaat atcatttaaa
      361 atcaatacga cggagtcaga cacggagtca aaacaagccc gctgagagta aaaaatatac
      421 agtcccatag gtaaagtgcc acaggactgt atattttttt atttgagatt acctgaaatg
      481 attccattta atttttatct cattttttcc tactagttag tatcaaatac tctcctattt
      541 tctcaataaa ctattttggg ttacatggta tctgaaacag ctaaaacctt gtcattatag
      601 caaaaaatca ctttatatcc gtttcatttc agatacattt cacttaagcg gattccaaaa
      661 aaacaaaaga aaaaggaacc cctagttagc ctatgctttt tattggttcc tctcaaaaca
      721 cggtcatata acaacggggt ggggtaaatg aatgaaaaag gtggaggaaa cattattcaa
      781 tagctgtctt aaatacttcg ttataaaatg tgaacaaata aaagacatct atccctattt
      841 tctacacttc cacttgacta ccatctttga atcgtacaat tttcttaact cttctcgttt
      901 acctcaattc attctcaagt ggaatcaact catcatctaa cattttacgc catgctcgct
      961 tcatttcttg tgggtattta aatggcttcg cttctggatg tttggcttta tatccttcgt
     1021 aatccttact gttgataatt gctttcatat taggaattgg atcttcacct ggtagatatt
     1081 gcagattaga agcatgtaat tctttctcag aaacaaacca ctcattggaa aagacatgaa
     1141 tcaaatcatc actcgcttgt gtaaaatagc gacgtttaat gacaaagata tcttcattac
     1201 tatctaaagt ccccacatca aatgcattta aaatttgtcg gtaaacttct ttgactcctt
     1261 ctgttttatt caatttagcc aaagcttttt caatttcttc tcgtgcatct gaactattcg
     1321 cagcataagt tcctagtaat tgatcaatat aagttgaatc aatatcatac aacttagtgg
     1381 tattttcact ataaaattcg atcgttgaaa aatcttctcc ttctttttct ggttcttcct
     1441 ctattaatga acccttcacg gtctcgtaca cgccaatttg ttcttccaaa agttgaattt
     1501 gattgttcaa agttggagat ttcaccatgt catcattgta atcgtcataa gtcactaacg
     1561 attcataagc acgattcaat tcttgaaatg ctttgacgta ctcgatacgg atttcaagtg
     1621 gcgtatgttc atcaatggat tccatggatg aaacctcttt taacttacca tatgcttgtt
     1681 tgaatctttt tttggattcc tgataacttg gataaatcaa acctgattct tcttttgctt
     1741 ttgaatacag ttttgtcgca tcctcgacat tcttctccat tgtcgctggc ttacgaaacg
     1801 tcacaatcaa tccttttgtc ttatcaggat acgtacgatt cgtacgtgaa aaagcttgga
     1861 tcaatcctgc atactctaga ttacgatcca caaatagggt ttgaatcgta ggggcgtcaa
     1921 aacctgttaa tagacgatcc actacaatcg ctaaatcgat ttgtttccca aattgtttga
     1981 attccgctct ttttcgtgcc aaacgattat tgatgtctcc gttataacgc tcaatgtctg
     2041 ctaatgacca ggaagtatca taatactgat tatattcctc aataattttt tgcatttctt
     2101 cttgttgggc acttgaatct cggctatttt cttctaatga ataggtaata gcgattcgtg
     2161 gaaaatcagg atcctccatc gttcgtcctt cacgaatgag ctgattggga aactcttttg
     2221 ttaaccaatc aggctctttc gtcatctctt tgatggcccg ataatacttt ttggccatgt
     2281 caatggaact cgtggttaag attgcagatt tcgttggcct gccatttcga aaatcaaatt
     2341 ttgtataggc attatctgga cggaacattt tatgaatgac tttttggata tgttcatctg
     2401 cttcaaaaac agctgggtcc aaataacttt cttttttgac aggttccatt tgatcaatca
     2461 tacgattgat ctgttctgat gaatactctg catatgtttc tacttctttt aatttccgat
     2521 agattttatt ctctagtgaa gtcggctcca cggtgttttc atgctctact tgaaatccta
     2581 agacagagcc atcttctagc gcattcttaa tggtataggt atgcaagacc tctccatact
     2641 gatcatgggt tgtacgtgcc aattgccctt tggcttgttt ccgattctca ggaaaaatcg
     2701 gtgtacctgt aaagccaaac caagtggatt tcggaaacat ttttttgatt tcttccatat
     2761 tttcagcact taacgctcga tgacactcat ctactacaaa cacgatatgt tggcccatta
     2821 atttttggaa gcgattggtt ccttttttct cttcttgttt ctttgcatat ttcaacgccg
     2881 catctagttt ttgacgggtt gtaatgatta caacatttgc attggcatct gccaacaggg
     2941 tttcacttaa ttctttggca cttcccgttc caacgatcaa gctattcgct ttggcgttcc
     3001 cggaagaaat cccagtatta aattccgatg caaattttgt aaactctgtg gtggtttgat
     3061 tatctaaatc tttgcggtcc actaacatga tcgttcgatc gatccccgat tttcgtgcta
     3121 aaagcttagt agatacaaaa ctagtcaacg tctttcctga accagtcgca tgccacacat
     3181 accctgattg atgtttattt gcggcaataa acaaggcttg aatcgcatgt acttgataag
     3241 gatgtaacac catgagggtt ttattgtctt gatcttcact gacgattgta taatccgcaa
     3301 tcaaacgatg agcatccggg atatttaaca cttgtttaca aaactcatat aagttttcta
     3361 ctttttcatt gtctttcgtt cgccaactaa ataaaaactt agggtgcaag tctttcggta
     3421 aggcatttgc aaaataccga gtggtttgtt cattggaaac cacaaacaac tgtaaggtcg
     3481 aaaagatatt gttccgaaac ataccttctt ctgcatattt tttgatttgg ttaaatgcct
     3541 gataaacacc atcttttgca gttacttgtt tcagctcgat ttggacaatc ggtaatccat
     3601 tgattaataa ggtgacgtca aaacgtcgat cccgtgcttc tatgttactt ccacgttttg
     3661 caatttgatg gaccacttca taagtagaga ttcctccacc aatgtcttga ttggaataaa
     3721 ggataagtga gacagaaccc aattgcgaat cttcccgttc aatcgtgatc cgagccatcc
     3781 cattttcacc ttttaaccat ttcgcagcat caaatggggt tttcgttcgc agcagtaatt
     3841 ccgttttgat cgtttcaaat tctttatcgg tcaaaggaaa atcattcagt tctgcttgat
     3901 tatttgagat aattttttgg cgtaaattga cccaaaggtc ttcttctgat tttagatccg
     3961 gacgatacgt ccactgatta tgcccttctc caagtacacg aatcaactgt tcttcgactt
     4021 ctgcttcctc acggtgacta attttcattc ttcttccctc ctagacaaac atcttttgta
     4081 agaagctttt tttcgtttct tttagtaaat ctaacttacg ttgatgaaga gcgatcgtat
     4141 catctagctg tttgaagaaa gtaccgattt tttgttgttc atcctttaca ggaaccttaa
     4201 cactgatatt ctcaatatca gaactgttat aagaaggcaa tgcccctact tgggagtatc
     4261 ttggcaaatt tatagtttca aacaatattt taccaaaata gatatcccaa gtattattaa
     4321 ataaataagc catggtattg ttatctatta agaaagggct tgtgaccaaa cgttttctat
     4381 taagcattat tgctgcgcca actttagcaa atataatcgc tggcacatct tttattggct
     4441 tccatttttt acgctcaatt tgctcattag atacataatg atttgcattt ctcatttcat
     4501 actcattacc aatgttattc atgtcggaaa ctttaaaaaa tggagtacct tcagaaccac
     4561 cttgctcggc atcaggaaat ccaattcccg attgagtttt acctaattct cccaacttac
     4621 gctcttccca atcttccgta aaaccaggaa accggatttc tggcactttt gctccgtttt
     4681 cagggaacat tttttgtaag aatccttttt tcgtttcctt cagcaaatct aacttacgct
     4741 gatgaagagc gatcgtgtcg tcaagttgtt tgaagaaggt gccgattttt tgttgttctt
     4801 cctcactagg aaacattgct actatatttt taaacttttt tagagacaga acatgttgac
     4861 ctgttccctc tgcttgaata gataattgtc gttggattcc attgttaata cgtcttaaga
     4921 agaagtcgga atcagcagat tttaaagtaa atacaggata aaaatagctt ataatccctc
     4981 tatcaataat gtcattacgg ttaaacacaa acacatcatt atcacttcga ctacggaacg
     5041 tgaaatatcc tctaggtagt acaaagtaac caatattatt ttctgtagtt acttgccgat
     5101 ttgcaaaata gtcttcttgc aaaactatcc ctttttgttg agatgaagtt agcataggaa
     5161 attgattttg tacacttgtc ttttcaacat aatctacaac gaagtccccc aacttcctct
     5221 gttcccaatc atctgtaaat cctgggaaac ggatttctgg ttgtgtatca ttgctcatgt
     5281 ttgaacacct ccagcgca
//
LOCUS       DACEDO010000116         5157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEDO010000116 DACEDO010000000
VERSION     DACEDO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.120"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(117..1079)
                     /locus_tag="IWO52_002687"
     CDS             complement(117..1079)
                     /locus_tag="IWO52_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0356912.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1081..2520)
                     /locus_tag="IWO52_002688"
     CDS             complement(1081..2520)
                     /locus_tag="IWO52_002688"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0356913.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2705..4651
                     /locus_tag="IWO52_002689"
     CDS             2705..4651
                     /locus_tag="IWO52_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0356914.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4915..>5157
                     /locus_tag="IWO52_002690"
     CDS             4915..>5157
                     /locus_tag="IWO52_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0356915.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1660 a          872 c          853 g         1772 t
ORIGIN      
        1 tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat
       61 atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa
      121 atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct
      181 ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt
      241 attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt
      301 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
      361 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      421 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      481 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      541 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      601 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      661 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      721 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      781 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      841 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      901 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
      961 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
     1021 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
     1081 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
     1141 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
     1201 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
     1261 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1321 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1381 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1441 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1501 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1561 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1621 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1681 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1741 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1801 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1861 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1921 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     1981 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     2041 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     2101 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     2161 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     2221 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     2281 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2341 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2401 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2461 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2521 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2581 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2641 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2701 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2761 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2821 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
     2881 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     2941 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     3001 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     3061 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     3121 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     3181 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     3241 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3301 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3361 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3421 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3481 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3541 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3601 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3661 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3721 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3781 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3841 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3901 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     3961 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     4021 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     4081 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     4141 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     4201 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     4261 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4321 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4381 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4441 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4501 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4561 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4621 tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt
     4681 tctttccaaa atcaaataat atagattaat caaatatttg agaataacat tttatttaat
     4741 cttttcatat atcaattgta tgaaaaatta ttagaatttt aaaaaatgat aaaaattcaa
     4801 aaagaaaaaa tagtagttat ttacttgtca tgttactaaa taaaaaaata atttaaatat
     4861 gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta
     4921 tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga tgaagaaatg
     4981 acgatcaagg aaagagtgag ctttccgaca acgacacctg aggaaacaat gcctttagtc
     5041 attgacttct tcaaacaata ccaagcagat ttagcaggga ttgggattgg ctcatttggg
     5101 cctatcgata tccatcgcga ttccgccact tatgggtaca ttacctcgac cccgaaa
//
LOCUS       DACEDO010000117         5137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEDO010000117 DACEDO010000000
VERSION     DACEDO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.121"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..447)
                     /locus_tag="IWO52_002691"
     CDS             complement(<1..447)
                     /locus_tag="IWO52_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0356916.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(754..1851)
                     /locus_tag="IWO52_002692"
     CDS             complement(754..1851)
                     /locus_tag="IWO52_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0356917.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1959..3986)
                     /locus_tag="IWO52_002693"
     CDS             complement(1959..3986)
                     /locus_tag="IWO52_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0356918.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4131..4862)
                     /locus_tag="IWO52_002694"
     CDS             complement(4131..4862)
                     /locus_tag="IWO52_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0356919.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1040 c          813 g         1828 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat attgttataa aaagagccca accatcctac
      721 aataataaaa atttactatt tgttatctta accttacttt tctttctctt ctttttttct
      781 caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag ctactaagat
      841 ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt ccttggctac
      901 ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt tgatcgtagt
      961 atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa cagtcattgt
     1021 aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa aagaaaaatt
     1081 tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt cagaatgtcc
     1141 tttttttgta atttttgttt caataactac ttgattgata taggtttttt gatcattttg
     1201 aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt catgaagagt
     1261 aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa tataggtgta
     1321 ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt ccgtctggat
     1381 ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg gcatcctgac
     1441 atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg gatagctcac
     1501 ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt ttccgtattc
     1561 aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt tcacttcttt
     1621 ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat caagttgaag
     1681 atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg tcactgaaaa
     1741 attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa taactaggag
     1801 tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca tatgtaagag
     1861 aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc tgtgcccttc
     1921 ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg gacatctgca
     1981 agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac aggaatattt
     2041 attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct ttcacccttt
     2101 tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt ttcagaggta
     2161 acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt ctcttgtttc
     2221 aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc acctgaaaac
     2281 tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt gacatattga
     2341 ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc ctctgtattt
     2401 tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc ttcaatatca
     2461 ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat ttcagcaata
     2521 tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt cagctttgcg
     2581 atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag gtattcaaaa
     2641 tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa ttcgattatt
     2701 aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt tccggttatc
     2761 aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg aatagttaat
     2821 tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc cccctcgacc
     2881 ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat ggccccataa
     2941 tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca
     3001 gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca atttacttct
     3061 gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagcgacc gacaccttcc
     3121 aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg
     3181 ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga ataattatta
     3241 ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc agcagttatc
     3301 tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc aatcgagagc
     3361 gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc catacctggc
     3421 gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa tgtaaaggtc
     3481 atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt aaatgtaaaa
     3541 tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga cctttctgct
     3601 gggttcaact gcagtaactt cccattttcc aatatggacg tgctagtacc gataggtgtt
     3661 tgaaaaattt tatcgataga aataatattg ggatcgtcag gcagctcaac cgcagaaatt
     3721 ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt ctacgttgtc agtggtctct
     3781 ccactactcg aaatacttaa atcctcactg ctttcttgtg tctggttgga ttctattaaa
     3841 ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc tatttccatg ctccaactga
     3901 ttcaattcca aagagtaccc tatgatcgga aagatattag tcactaagag actgcttaaa
     3961 atgagcacga tcctcctctt tttcaatata tcttttcctc ctgatcgtta aattcctaga
     4021 tgaactattc gtatccaaac aaatttagcc tagcctttga caatttttgc tcgattacct
     4081 cgcaagacaa tatgactacg tatttatttt taacagttct tttcagtaaa ctagttgtta
     4141 ggtgtatctt ctaatgtcca aactaattta gtggaatatt gttgcgctaa cttgacagat
     4201 ttaccgggta ccgaaagctg taccgcatct tgattttcat tttcatttgc accaaatcga
     4261 tagacccaag tccccacacc tgttcctgat ttagcattaa ttgctaacgt attgtttcct
     4321 cccggtgtca acgttaattc agaattcacc gtttgcggcg tatatgttgg atcaaccaaa
     4381 gaaacagcct gccctttcgt aaatttcagc tgtgctccaa ctagttcatc cccactggca
     4441 gttttaaact gttctggttg agagacactt aatttccacc cagataaggt acttctccta
     4501 tctgtcactt ggacatatgt cggttttcta gacccatcgt tcatcacatc agcagctgca
     4561 taatacgttt tatccgcagt agaaattttt tgagtcccaa atttgaattt tgatccataa
     4621 tcaatcgata atgcccctcc tgttccaggg cttggaggat ctgatggatc gacaggttta
     4681 ttgggatcag gattctctgg atcaacagga ggagttacac cctcatctcc tgcttcaaat
     4741 gtaatcgttc cttctgtgtt atattccttt gggtatactt cagttgcaga aacaaaagtc
     4801 gtacttgcta ccatgctgaa taacatcatt gctgctgtta atacgatttc ttttttcatc
     4861 attgacattc ctctttcttt atttactaaa catatcagtc gattggatta atccatagca
     4921 aaattctgtt ttacgatact tatgaaataa aagaacatag acctatattt ggtcctatgt
     4981 atcgattagt tataaatgat tatcagcccg atttgatttc tagtttttat tgcaacttga
     5041 tgcaaacaca atctagaaat acagctgtca tagcctttaa aataattcgt aagtccccct
     5101 cctatctatc aaaatgtgac ttgacgagag tgtaaaa
//
LOCUS       DACEDO010000118         5063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEDO010000118 DACEDO010000000
VERSION     DACEDO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.122"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            34..1175
                     /locus_tag="IWO52_002695"
     CDS             join(34..262,262..1175)
                     /locus_tag="IWO52_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0356920.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1354..2640
                     /locus_tag="IWO52_002696"
                     /pseudo
     CDS             1354..2640
                     /locus_tag="IWO52_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
     gene            2720..3115
                     /locus_tag="IWO52_002697"
     CDS             2720..3115
                     /locus_tag="IWO52_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356921.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3440..4057
                     /locus_tag="IWO52_002698"
     CDS             3440..4057
                     /locus_tag="IWO52_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0356922.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4098..4397
                     /locus_tag="IWO52_002699"
     CDS             4098..4397
                     /locus_tag="IWO52_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356923.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4431..4598
                     /locus_tag="IWO52_002700"
     CDS             4431..4598
                     /locus_tag="IWO52_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0356924.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4643..>5063)
                     /locus_tag="IWO52_002701"
     CDS             complement(4643..>5063)
                     /locus_tag="IWO52_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0356925.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1738 a          808 c          991 g         1526 t
ORIGIN      
        1 gaatctaact actaagaaaa ggacgtgtcc acgatggcaa aagccaagaa aaaatttgat
       61 gaagacttta agaagatgat cttagattta aaccagtctg gccaatcggt cgaagaactg
      121 gcagcgcaat atggtatcgc tacacaaacg atttatcgtt ggaagaagct tcatacaaag
      181 aatgtagcaa ccggcatgac tgaggcagaa atcctagcga tgaaaaagga aatggcccgc
      241 atgcaggagg aaaacactat cctaaaaaag gctttaacca tattcgctca aaagtaaaaa
      301 tgagtgatgt cttcgaattt attcaatcaa atgttcatga tcatgacgta aaacaaatgt
      361 gtaccgtttt ggaagcaccg aaatccagct actatgactg gaagaaaaag aaaccttcca
      421 agcgccaatc agaaaacaaa cgcttaaacc gcttgatttc aggaatctac tttgaaaaca
      481 agggtatcta tggtgcgcct aaaattcata agattcttgt tggccgtggc gagaccttgt
      541 ccttaaagaa agtacaaata ctgatgagaa aattaggctt gcgttcagtc accctcaaga
      601 agtacaagcc tggcaaacaa gcgaaagtta aaagtgaagg acgtaaaaac ctgttaaacc
      661 aagattttac cactacgtcg atcaatcaaa aatgggtgac agatattacc tatattcata
      721 ccctaaaaga tggctggacc tacctctcca ctattcaaga cctccacaca aagaaagtca
      781 tcggttggaa gtttggcaag cagatgacga aagaattggt tatcgaaacc cttgacaatg
      841 cgctcttaaa ccaaacaccc tccgagaact tgatcatcca ctcagatttg ggttctcagt
      901 acactagcga agcctatgag gcaaagctga atgaattgaa cattcggcac tccttcagtc
      961 gtaagggctg tccctacgat aatgccggta tcgaatcgtt tcacgcctca ctcaaaaaag
     1021 aagaagtcta tccatcaaaa gcgtatgaaa actttgaagc tgcttattta gcgttatttc
     1081 aatacattga aggattttac aatcgcaagc gaatccatag tagtatcaac tatttaacac
     1141 caaatcagat ggaggagttg gcattgcaag cttagttcct agctaccact tacatttttt
     1201 tggcgagatt tccacagggt ttcaacttca tcgcttcgat cagcggtcaa acggttttgg
     1261 tttgaccgct ggtcgaaacg atgagatact cacccgctgg aatctcgaaa aaattctcac
     1321 aaatcgtgtc cgaggtattg actcaaatcc agtgcagcat tagcttattt tacttatgat
     1381 cgtggcttat cagatggtaa gaaggctata gatgcagcaa aaaatctaaa tataccatta
     1441 acaacagtta tttattttgc tgttgatcta gacatgttag gggaagaaat tcttgcatat
     1501 gctggagaat attttaaggg ggtatctgct gtaatgtcat atagcgggta tcaaacgggt
     1561 gtttacggaa caagaaatgt ttgttctcaa ataatcaata acgggtatgc ttcgtttgca
     1621 tttgtttcag atatgtcgac tggttatagt gggaatttag gttttcctat gcctagacaa
     1681 tggtcttttg atcaatttgt tgaatttacc ataggaagtg gcaacggagc tgttggtatc
     1741 gatttaatcg ctacatcggg tagagatagc ggatttaatg agttatcaaa tgatactaat
     1801 aatgataatt acattgccaa atacaatcag aaggttataa atcagatggt acgtgcgtat
     1861 caatatctat cacaagctgg tcttgataat ccttggaacc ctcatttgac attttataga
     1921 tatgtaaatt attctggttt gtcatgggac atcatttctt ctcctgtaac agagcatgat
     1981 agaaaaattt atgatgaata taggaataaa ttaactaata gcgagggcct gtataattat
     2041 tttatagatc ctaatactgg ttcaataata ggtttgcctc acttaattgt gacgttacaa
     2101 tcacagatgt ttctagccga cacaatcaat gatagtgttt ctgactttgc aggttggtta
     2161 ggagatttaa tgacttgctg gggtgaagtt aagaaattag gaatacaaat ggagcaaggt
     2221 gtatttgcat tagtaggtac ggctgcaggt ggaacattta gtctagaaga tttataccaa
     2281 gatgttgacg cggtaaatgt ttatacctta gcaaaaagaa attctgattc aacagcttta
     2341 tcggctttaa aaacgtacta ttgtgataag ggtgctattg atagatttaa tcaatttgtt
     2401 aagaacagat ttggttcttc agataaaatc gcttcagtaa caaatagttt attagaaaat
     2461 gacgaaacag ttgatatctt aggtgtaggg ataatttcat ttttcattga taaaatagtt
     2521 aataattcac ttgggacaga ttttacttat cttgatttta ttggagatga ggggaaagtt
     2581 gcaaatggat tttctgaaaa aattattcaa tttcaagaaa atagaagtaa acttaattaa
     2641 gaatcgttta ctatttaaac agtgtccttt tcctttttaa atatatacga taaaataagt
     2701 atatgaattg taaaaggaca tgatgattat ggaagaaata gaatggaata atctgactca
     2761 aaataataaa aataattctg gatgtggaat tatctttatg tttgttatag ttttgggaat
     2821 agtagtactt ttaataactg tcaagatagt agattacttt gcgaatactc ctgaagaaca
     2881 aatacagaga aaacttcctg agtttgaaag agtaccacct tatgatccaa gagataattc
     2941 atttcaagta aaaaaattaa aagagtctcc aaatgaaatg tggtttgaag ttacagttga
     3001 atcaagcgac ttcaaagaaa aaaagaaaga atacaattat attgtaaagg caaaagtatc
     3061 aaaaagattt atttttaacc ctaaattaga aatatatagt gttgtaatag aataatagtg
     3121 ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg tgaaaaataa aatataccat
     3181 ggaagacata aaagaaaaac tggataacta gtttatttaa tggcagaccg ttaaggcctg
     3241 ccatttttat gtattttagc ggaataaata taatcatgcg ggagtgtaaa cttactgttt
     3301 caaaaagtat actttttgac atgccttgat tttctttgtt tatgtatggc ataataacca
     3361 cataatacaa tttgaaacgt ttctgattta atctgattgt tttgaaacaa ttttagtgaa
     3421 aaaagggtag ataaaaacga tgagaaaatt tttttatatc cgggtcagta gttatgatca
     3481 atcgatcgaa cgacaactga tttccgcgaa ggaattagga atccctgaag aatatatatt
     3541 tgtagaaaaa gccagcggca aagattttaa acgccctgaa tatcaattaa tgaaacggat
     3601 gtttcgaaaa ggagatttac tctatctaca atcattagat agactaggca gaaataaaca
     3661 aatgatttta gaagaatggc gagaattaac taaggaaaag cagattgata tttctgtgtt
     3721 agacatgcct ctgttagata ctgctagtta caagcaacta gatgggttag agacgcttat
     3781 ctctgactta attctgcaat tactcagcta tatggccgaa gacgagcgga aacgaattaa
     3841 tagccggcaa agagaaggcg tagaagctgc gaaacaaaag ggtgtaaaat tcggcagaaa
     3901 gaaaattgct ttacctcctg aatttcctgc gatctattct gactggaaag caggaaagat
     3961 aactgcagtc tatgctatgg atcaattggg catgaaacga aatacctttt atcgacgggt
     4021 gaaagaatat gaacatacct tgcaccactt ttcttgatag atataacagc tattagacgg
     4081 gaagggagag tttgaaaatg gacttaacga atcaaagggt cgtttttact ggaacgctgc
     4141 agcaaatgac acgaacccaa gccatcggtt tggttcactc tttaaacggc cattgtcaat
     4201 caagtgttac ttcaaaaaca aatttcctgg tttgtggtca gtattctaag tcgttgtttg
     4261 atgattctct ctatacaaaa aaggaacaaa ccgcgcggga cttgattgcg ttaggtcatg
     4321 agattaccat tttatcagaa gtcgaatttt atgcgttaat tagtcaacag ttgaaagaat
     4381 ggcaacggta tttataatcg ttagtaaaat ttacaaggag gaaaattttt atgaagcgaa
     4441 aatcttatga tcgttggggc attgaaataa ctagttttaa tcgaaatatt gcttttcacg
     4501 tggagtgtga ctgtggggaa ttagcaaaaa gtgtttatca aaaaaattac ttttactgtc
     4561 ctaaatgcca tcgccgatac ggggtacatg gaacatagaa acgaaatagt gcggtagaaa
     4621 agcgaatgaa tgtagtatat ttctacaaat cattcgcttt ctttatttta taaatagttg
     4681 gtcgagagat cccgttttct tttgcaatct ctgcaactgg aatgccttgc tttaacatgt
     4741 ccaccacggt tttaaaaact aagcgttttt gcggatctgt tgagttaata gaatactcga
     4801 ttggtcgtcc cttatatcgg ccggcttttt gagccaataa tactccttgc cgctgacgtt
     4861 cacgaatttt ctcacgctcg ttttgtgcaa tataacttag cagtgataaa aacatatcga
     4921 acatagcggt atccaggagt tcatttccag tattaaaatc taaaaactgt gcatctaata
     4981 tttttaacgc tacttttttc tgtttcataa aagcaactgt attttttata tcttcataat
     5041 ctcttcctaa acgatctagt gat
//
LOCUS       DACEDO010000119         4980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEDO010000119 DACEDO010000000
VERSION     DACEDO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.123"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..259
                     /locus_tag="IWO52_002702"
     CDS             <1..259
                     /locus_tag="IWO52_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0356926.1"
                     /translation="GITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            341..1195
                     /locus_tag="IWO52_002703"
     CDS             341..1195
                     /locus_tag="IWO52_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0356927.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1292..3541
                     /locus_tag="IWO52_002704"
     CDS             1292..3541
                     /locus_tag="IWO52_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0356928.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3544..4719
                     /locus_tag="IWO52_002705"
     CDS             3544..4719
                     /locus_tag="IWO52_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0356929.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4722..>4980)
                     /locus_tag="IWO52_002706"
     CDS             complement(4722..>4980)
                     /locus_tag="IWO52_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0356930.1"
                     /translation="GITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1737 a          725 c          907 g         1611 t
ORIGIN      
        1 gggcattact atatccgttc cgccacgtaa aaacatggat aaatcagaaa aactagacca
       61 cagcttactt ggaaaacaaa gaaaaacagt tgagactgtt ttttcttcct tagaaaaatt
      121 aggttgtcaa aatttcaact cacgttctgt taaagggcta gagagtagat ttgaaagcat
      181 attactggct tacagtgttc tattaagtcg agcacaacga cgttttgaag gaactttgag
      241 atattcttta ggatattaaa attaactagc accacgggtt atgtaataat ataaacataa
      301 taaaacaaaa attatccaaa tctaacatag gagggtggat atggaaaatt ctatatattt
      361 agtaacagaa cctaacactc agctgaaaga ttgttacttt aatttttgtg ggtattcaaa
      421 aacagaacct agacatagtt ttggaccagc tattcgagaa caatacttaa tacacatagt
      481 gctagaaggt gaaggctatt attcaattaa tgatcaaaaa tattttctga agaaaggcca
      541 aggatttgtt atcccaccag gagtatctac tttttatcaa gcagatgaaa aaaatccgtg
      601 gtcatatgta tggttgggtt taagtggcaa tcttgtagaa tactatctaa aagaaattgg
      661 tgttggaaag aatcatctta cctttaaagt cgaaaattta aattcattta aaggattaat
      721 ttttgaaact ttatcgtatg atcaggacaa tctagtcagt gaattgtcca gacaaaaaca
      781 agtttataaa tttttagaac tcctcttaaa ttcagctagt tatctgaacg aagggataat
      841 tactaaaaag atgaatcctt ttgttcgtca aacaattgaa ataatacgtg agaatgcgta
      901 tcaaaaatta tctgttaaag atattgctgt cagtctatcg atacacgaaa attatttatc
      961 acgattgttt aagaatgata tgggaatatc aataaaagag tatattagtg aaatcaaaat
     1021 ttctattgca aacgatctgt tatcatcaac tgaatattca atccagcaaa tcagtgacaa
     1081 gataggattc tcaacagtac aatctttttc taaagccttt aagaaagcaa atggtgtttc
     1141 accgggagta tatcgaaaac gtagattagg tctaggaatc attaatggtg aataaactaa
     1201 acatttgatt ttgataaata ttcgttactt taaacgctta ctatttatgt gagcgttttt
     1261 tataatgcaa taaagaaacg gaggagagct tatgtataag gataatgaaa tgaatattat
     1321 ttatcgatca gaaaaccaac aatttcattt gtttaatcag gagataagtt acatttttaa
     1381 gatatcggaa gatggtaaat tattgcattt atattatggt gagaacttac cagataatga
     1441 ctatagtcat ctaattgaga tgcatcatcg gcctatgaca acctatagaa atgaaaacga
     1501 tttgctatat tcgttagagc acttaaaaca agaatttcct gaatatggta caacagattt
     1561 tagacatcct gctatttcat tactacagaa aaatggctct cgcttatcag attttgtcta
     1621 tcaagggtac gaagtggaag aaggaaaacc taaactagaa ggtttacctg ctacctatgt
     1681 tgataataat gatgaaagca aaacattgaa agtttatatt ttagattcag ttacaaatgt
     1741 tcaagtagaa ttattatata caatttatag agattatcct gttattactc gtagcgcgcg
     1801 agttatgaat aaaggaaaag atattcaaat aatcgagaat attagcagtt tatcattgga
     1861 tttacccgat gcaaattatg aatggcaaca attatctggt gcttggggta gagaaagaac
     1921 tattaagtcg cgtactttac aacaaggaat tcaatcaatc gaatctactc gaggtatttc
     1981 aagccatcag cataacccat ttgttacttt aaaaagaaaa aatactgatg aaaatcaagg
     2041 agaagctatt ggtgcagcac tagtttactc aggaaacttc ttaattcaag cagaagtaga
     2101 tacatgggat gttactagac tacaagttgg catcaatcca tttggattta attggaaact
     2161 caatccagga gaaatattta cagcaccaga agcgattctg gtttattcaa gtaagggttt
     2221 aaatgccatg agtcaaactt ttcatcaact atttagaaaa cgcttagctc gtggtaagtg
     2281 gcgagaaaaa gaccgtcctg tcttaattaa taattgggaa gcaacatatt ttgattttga
     2341 cgaagaaaag ttggtaaata ttgctaaaaa agcatttgat gttggtattg aattatttgt
     2401 attagatgat ggctggtttg gagaaagaga aaacgattgt gcaggattag gagattggca
     2461 tgtaaatcct aaaagactac ctagaggaat cggcagcctt tcagaaaaaa ttcgtagttt
     2521 tggtatgaaa tttggtttat ggtttgaacc tgaaatggtg aataaggata gtgagttata
     2581 tcgcacacat ccgaattgga ttatttctac acctaatcga aaacctaatc atggtaggaa
     2641 ccaatatgta ttaaattttg ggttagatga ggttgttgaa aatattttcg atcaaatgtg
     2701 taaaatcatt gatgaatcaa acttagatta cattaagtgg gatatgaatc gtccattgac
     2761 tgatgtattt gacagtcact tgtcagcaga tcaacaagga gaagtattcc acagatatgt
     2821 actaggcgta tatcgcttat atgaaaaact tataacaaaa tatcctaaca tactatttga
     2881 gtcttgttca tcaggtggtg ggcgctttga tcctggaatg cttcactatg cacctcaagc
     2941 ttgggctagt gatgattcag atgcaattga aaggttaaaa attcaatatg gaactagtat
     3001 gctgtatcct ctttcgtcta ttggtgctca tgtatcaatt gttccaaatc atcaaacaaa
     3061 ccgtataaca cctataaaaa ctagaggaaa tgtagctttt tttggtgcat ttggatatga
     3121 gcttgactta aatgaattaa gtgaaggcga tttgaggatt gtaaaagaac aaattgcctt
     3181 ttataaaaaa tatagaacca taattcataa tggtactttt tatagactat tatcaccatt
     3241 cgatgatgat aatcaaaccg cgtggatggt tgttagtcat gataagaaaa cggctattgt
     3301 tgctcattat aaaacattaa atgaggtaaa tgctccttac cgccgactga aattaaaagg
     3361 gttagataat gacggacttt ataatgtagg acaaaatagt ttgcgatctg gaatggaatt
     3421 gatgagagca ggactagtct gttcagatgc atctagtgga caaataaatg atcagaatcg
     3481 acgcccagaa acatttgatt ttgattccaa gatttgggtt ataacacagg aggaaaagta
     3541 gagatgaata accaaaaaag cttacttcaa aaaggagcaa tcttaagtat ttcactaatg
     3601 ttaacaagct ctcaagcaat taatggtgtt attccacaaa tgaaacagtc tttggggata
     3661 tcacaaagtc aaagtgaact tttaggaact gcaccaagta taactgttat tttgtttatc
     3721 ttattatcta gttactttgc caaaagaata ggaatgaaaa agacaataat tttaggttta
     3781 ttattagctg gtataggagg aattattcca gtatttacag ctaccttcca aaccgttctt
     3841 attagtcgag taatattagg tgccggttta ggagtatata attcattggc tgtgacatat
     3901 atcagctctt tatactctgg caatactaga gcaacgctat taggtattag aaattcaatg
     3961 gaagctttag gtcagacaat ccttattttt cttgctggag ttttagtgaa cattaattgg
     4021 acggcttcgt ttttagtcta tgcaattgcg tttcctgtag ctctcttatt cgcgctaaaa
     4081 gttcctgaaa ttagagatga aaatagtgac atacctgaaa atcatgtaaa agaaaagatg
     4141 aatccaatgg tttttgccct agttctattt gcaatcttac tcgtaatgaa tagtattgct
     4201 atttctgtac gatttgcatc aattgcaact gaaattaaag gggtaaactt taatgctagt
     4261 aactatttag ctctaatgcc tatcttagga atactagcag gatttatgtt tggaccaata
     4321 aataagtgga taggaaaagg aaccctatac ttgggaatta tattctttat aatttcaaat
     4381 ttgttaattg ctgtgtcaaa cggtaatatg acgttcctgc taacgggttt atttttatca
     4441 agtataacag gatcatggtg cttcccattt atctttagta acttagataa agtcactaca
     4501 aagaatacaa ttaattttgc tacttcattg atttttattg gctgtaatat tggtaatttt
     4561 atagctccta ttgctatgca attagcacaa tttctaacaa gaagtacaaa tttgactgct
     4621 ccattttttg tattcgcagg aatatttatt gtgatcctat ttgtaacatt ttttgcgaca
     4681 aataaaaaaa atactagagt aacccgtggt gctagttaat tttaatatcc taaagaatat
     4741 ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata gaacactgta agccagtaat
     4801 atgctttcaa atctactctc tagcccttta acagaacgtg agttgaaatt ttgacaacct
     4861 aatttttcta aggaagaaaa aacagtctca actgtttttc tttgttttcc aagtaagctg
     4921 tggtctagtt tttctgattt atccatgttt ttacgtggcg gaacggatat agtaatgccc
//
LOCUS       DACEDO010000120         4798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEDO010000120 DACEDO010000000
VERSION     DACEDO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.124"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..104
                     /locus_tag="IWO52_002707"
     CDS             <1..104
                     /locus_tag="IWO52_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAQ0356931.1"
                     /translation="LLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            792..1613
                     /locus_tag="IWO52_002708"
                     /pseudo
     CDS             792..1613
                     /locus_tag="IWO52_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1756..1980
                     /locus_tag="IWO52_002709"
     CDS             1756..1980
                     /locus_tag="IWO52_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0356932.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            1995..2864
                     /locus_tag="IWO52_002710"
     CDS             1995..2864
                     /locus_tag="IWO52_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0356933.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2845..3579
                     /locus_tag="IWO52_002711"
     CDS             2845..3579
                     /locus_tag="IWO52_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0356934.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3612..4520
                     /locus_tag="IWO52_002712"
     CDS             3612..4520
                     /locus_tag="IWO52_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0356935.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4517..4765
                     /locus_tag="IWO52_002713"
     CDS             4517..4765
                     /locus_tag="IWO52_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAQ0356936.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1632 a          718 c         1050 g         1398 t
ORIGIN      
        1 ctttgctatt agttatggat ttgcaaagtc accttattga acaatacaac ttaaaagtta
       61 cacatgacat tttagaaaga ttgttgaatt acattagtga ataggagagt aacgttattc
      121 acagtattgg gtactgaaaa ttaacagcat acctcagttt ttcaaaaaac agcgaagcct
      181 atggatatgt tcccgtctac cgatagtgca aaaagtgtcg tggatatgtt tccgagaacg
      241 gacatacaca aatgacattc attctgtcct ctcaatccat gtggaagcag tatgtctgtt
      301 aacaagaaaa taattattgt tcgtcctgac gagttgttag actcgaaaag gataaacatc
      361 atttttagaa ccggtagccg atttttggct agcggttttt tgggctcttt ggcaaatagt
      421 ggtgattgaa tctcgcgcta tcctatggaa aaccgccttg tgtgaggggc tgcgagaccg
      481 cttcgatcct agaattgctg aagcacgaat ctagcgtcac tcacatttca tcttttgacg
      541 tagctgagac aaggtgtttt tcttcgatcc gctagtggtt aatctctttt tggtgcaaat
      601 atggtttaga atttctttgc atggcttaag ggagtggaga atcggatatt agtaggattt
      661 acatccacag ataaaaatag gcttgatttt aacagcgaaa ctaaaatacc ctacttaaat
      721 gaagtgatag attagatgtt gggttgttag gagatgctta aaaatcctat gcaatcaata
      781 gtttttagtg tttgttgact tgatccctaa atcgcctgag tactaaacgt tgagtgtgtc
      841 tgcttactgt cactagccag taagtgagac tgtagttaat ctctcatttt ccccagtatt
      901 atgttgaaaa tagccatgaa gcgattattt ctaaagagtt atttttgcag gctcaagaag
      961 aaatttatcg gagaagtaat atctacacag gagaaggcaa gaacaaaaga atttatagta
     1021 gcaagtacgc tttaagtgcc atcaccttct gtggatattg tggcgatatt tataggagaa
     1081 cctattggaa tattcatggc agaaaagaat ttgtctggcg atgcgtgact agaatcgagc
     1141 agggtcccga ggtatgtaag aaccgaaccg taaaagaaga tgaactctat ggtgcggtaa
     1201 tgaccgcaat taataagcta cttgctggtg gcaacaatat gataaagaca ctggaagaaa
     1261 atattcatgc tgtgattgga gaaaccacag aataccaaat ttcagagatt aataccctac
     1321 tagatgaaaa acaaaaagaa ctaatcaagc tggccaataa aggtcaagat tatgaatacc
     1381 tggtagatga gatcgatgag atgcgagata agcgtcagac tcttttagta gaagatgcct
     1441 ccctcagtgg cgagaacgag cgcatcaatg agttgattga atttatccgc aagaacaaat
     1501 tccgcacctt agaatatgat gataagcttg taaggaagat aatccagaac gttaaggtct
     1561 atgaagacca cttcgtcata gccttcaaac caggtatcga aatggaaata tgaaaaccag
     1621 aagtaaatag cccatgactc tgaagtagag ttgtgggttt tcttttaatt gctgattata
     1681 ataatatttt aatgactatt gcatttatca accaaatggt ttatgttatt cttagcataa
     1741 acttggaggt gctgtatgac tacggcagaa atgattaaag aactgtgtga gcaaatgaat
     1801 ataagtgttt ccgaacttgc tagacgtatt ggccagactc cacagaattt taataaaaaa
     1861 ttacaacgag aaacagtaac cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc
     1921 aagtttgtgc aggcatttat tttaccagat ggagatgaaa taaaaatatc taacgaataa
     1981 aggaggatgt ttatatggta ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt
     2041 tgccttttat agaaggcatt gtattagggg gctcacgtgc aagaagcacc catacagaaa
     2101 attctgatat agatatcgga atctattaca attcagactc atttgacctg acagcgatta
     2161 atcaaattgc tacagagttg gatgatgaga atagaaacaa ccttgttgta cctcccggag
     2221 cgtggggtga ttgggttaat ggcggtggat ggttagttat aaacgggtgt catgttgact
     2281 tgatattacg tgatataaaa cgggtggaac aaataatcaa agatacggag caaggaattg
     2341 ttactgccaa ttatcagacc gggcatcccc atggttatat aagtgctatg tatcgtggag
     2401 aattagcgat tagtaaaata ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc
     2461 aggcagaaat ttaccctact gctctaaaga aaagcttgat gaactttttt atatttgaag
     2521 ctgaattctc tttaatgttt gtaaaagcaa atgcgggggc agaggataaa tattatattg
     2581 caggccatgt ttttcgtatt atttcatgct taaatcaagt actatttgca tgtaataatg
     2641 cttattgcat taacgaaaag aaagctataa aactgcttga aacttttgaa tataaacctg
     2701 aaaaatatgc cgaaagagtt aatcatattt ttgaagtact cggtctttct ctttttgaat
     2761 gctacgacat gaccgagaaa ctttataaag aagtgaatga aattgtatcg gagataaata
     2821 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     2881 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     2941 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3001 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3061 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3121 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3181 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3241 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3301 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     3361 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     3421 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     3481 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     3541 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     3601 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     3661 aggatgaacg tattcgaatt gtgacccttg aggggtcacg cgcaaatatt aatataccta
     3721 aagatgaatt tcaggattat gatattacat attttgtaag tgatatagaa ccgtttatat
     3781 ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag ccggaggata
     3841 tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt gatgattaca
     3901 ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta aagggcgata
     3961 aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata gttccgactg
     4021 atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc tgcaatgaat
     4081 tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt ttatttgcta
     4141 ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca tggaaggtcg
     4201 gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt gaaaggtata
     4261 tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc tatgaaaaca
     4321 tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt gaggtggcgg
     4381 aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat accagggaca
     4441 tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc gctgatatag
     4501 aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag atatcgataa
     4561 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
     4621 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
     4681 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
     4741 tcttttaacc cgtggtgcta gttaatttta atatcctaaa gaatatctca aagttcct
//
LOCUS       DACEDO010000121         4709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEDO010000121 DACEDO010000000
VERSION     DACEDO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.125"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IWO52_002714"
                     /pseudo
     CDS             106..357
                     /locus_tag="IWO52_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IWO52_002715"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IWO52_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0356937.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IWO52_002716"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IWO52_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IWO52_002717"
     CDS             1786..1869
                     /locus_tag="IWO52_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0356938.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWO52_002718"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWO52_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0356939.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IWO52_002719"
     CDS             complement(2994..3857)
                     /locus_tag="IWO52_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ0356940.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IWO52_002720"
     CDS             complement(3859..4131)
                     /locus_tag="IWO52_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0356941.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IWO52_002721"
     CDS             complement(4148..4363)
                     /locus_tag="IWO52_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ0356942.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4709)
                     /locus_tag="IWO52_002722"
     CDS             complement(4455..>4709)
                     /locus_tag="IWO52_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAQ0356943.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          795 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgat
//
LOCUS       DACEDO010000122         4590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEDO010000122 DACEDO010000000
VERSION     DACEDO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.126"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            148..1137
                     /locus_tag="IWO52_002723"
     CDS             148..1137
                     /locus_tag="IWO52_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0356944.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELIESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IWO52_002724"
     CDS             1134..1553
                     /locus_tag="IWO52_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356945.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IWO52_002725"
     CDS             1562..1870
                     /locus_tag="IWO52_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356946.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IWO52_002726"
     CDS             1874..2236
                     /locus_tag="IWO52_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356947.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IWO52_002727"
     CDS             2458..3864
                     /locus_tag="IWO52_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0356948.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            4313..4432
                     /locus_tag="IWO52_002728"
                     /pseudo
     CDS             4313..4432
                     /locus_tag="IWO52_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT         1598 a          651 c         1042 g         1299 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgatcgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagcttaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggccagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttca
     4021 aagggtgcgg ttttatgttt ttggatattt gcatagctaa agagaagtgc tattattgag
     4081 ttatcaaata aataaactga aaaaagagca aaaaaataaa gtcgagcaac gctggacacg
     4141 ttctcgacct ttacggaaac taatcacact agctccgtaa ctcaaattca ttatacgaat
     4201 aatttcaggt ttcttcaaga ctagagattg ctggttatga attttttaga gttacgtgaa
     4261 ctctggaaaa ttctttccac caatctcttt tttctttgag gaggaactaa atttgagtaa
     4321 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
     4381 tgtaaaaact ttttacaccc ccttaaaaat gcagtggtcg attgatcgaa ttttatgtct
     4441 ttgggttttg gggttttaaa acgaaaaaaa gggggcgatt ttcgccctct tttttgatgt
     4501 tttttcttat cttgatacta taggcaacta tttctgcgaa gaaatcgggg ctatagtatg
     4561 ggtctatccc ttgtggctct aaggaagaac
//
LOCUS       DACEDO010000123         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEDO010000123 DACEDO010000000
VERSION     DACEDO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.127"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWO52_002729"
     CDS             complement(543..2048)
                     /locus_tag="IWO52_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0356949.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWO52_002730"
     CDS             complement(2045..2731)
                     /locus_tag="IWO52_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0356950.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO52_002731"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO52_002731"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0356951.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACEDO010000124         4426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEDO010000124 DACEDO010000000
VERSION     DACEDO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.128"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(279..809)
                     /locus_tag="IWO52_002732"
     CDS             complement(279..809)
                     /locus_tag="IWO52_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0356952.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            914..1465
                     /locus_tag="IWO52_002733"
     CDS             914..1465
                     /locus_tag="IWO52_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0356953.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1618..2091)
                     /locus_tag="IWO52_002734"
     CDS             complement(1618..2091)
                     /locus_tag="IWO52_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0356954.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2229..3701
                     /locus_tag="IWO52_002735"
     CDS             2229..3701
                     /locus_tag="IWO52_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0356955.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3698..4408
                     /locus_tag="IWO52_002736"
     CDS             3698..4408
                     /locus_tag="IWO52_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0356956.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1390 a          807 c          851 g         1378 t
ORIGIN      
        1 caaaacgctg atttcacaac ctcttgataa acggtgttca aacggctgct tctcggtaat
       61 aagcccacta gcctagaaaa tatgaaaagc tattttctaa ctagcgttct tattactcaa
      121 agcataacgc tgatttcaca acctcttgat aaacggtgtt caatcagctg accctcggta
      181 ttagctcaaa aaacggcaaa acatgaggaa ctatttttcc gtttctttct ctaatactca
      241 ggtcagaacg ctgatttcac aacctctctt tcacaacctt attttactcg tttataattt
      301 tcatcgaagt attttccgct acggatatca tctggcattc catcgtatgg ttcttcgcta
      361 ataacatctt cattgtctat acctgcatca cccatataag tgatcgtagc aaattcagga
      421 acaagtaatt ttggataagt ttcatatttt tcacgagctg cttccatttc tgcgatatgt
      481 tcgttttgga aagtaacagt gatttctgga tgttcttcaa cccatttagg gaaaaagatc
      541 attccgtgaa gtttcttttc atttggcata aagtccaatg ccacgtctgt ccctgttggc
      601 tctgtccaag tgattttgaa tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct
      661 tttacccaac ggcctttgac cattccgcca tggatacggt aatcaacagt atggtcattt
      721 ttggcatacc attcatattc ccaaccattg tcatacgtat agataaagtg agttccaata
      781 aaatcttcca attctttaaa ttctttcatg tttaagcatc tccttataaa ttaaaacatg
      841 taataaacta catgttttcg attactcatt tatgatagaa tggatttaaa gatttgtcaa
      901 ggagggaaaa ctgatggcaa gaaaaaatac acttcgatat attttgttag ggttattaag
      961 taagaagaag atgacaggat atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt
     1021 ttggcaagca aaacatagtc agatttatcc tgaacttgca aaaatggaag agcaaggaat
     1081 cattcagcat caagtagaga tcacaggtga aaaattggaa aagaaagttt atgaattaac
     1141 agaagaaggg aaggaacaat tggcagagtg gattcataca cccaccaatg aactaccggt
     1201 taatcgagac gaatttgttc tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt
     1261 agtagaaatt attcagcagc agcgtgacct tcatgaagaa aaacgacttc atctattaga
     1321 gcgaaaaaaa acgatctttc ctactgaaaa agaaaaacat gaaaattacg gacactacct
     1381 gattttagcg catgcgatca atcgggaaac agaatatgca aattggctaa cagatgtatt
     1441 agaagaaagt aaccgaaaaa aataaaaaaa tatccgaaca gtgatggaga attcctttct
     1501 atatgaaaga aacgcagatc accgttcgga tattcttatg ttcaaacagg ccttttttca
     1561 agtatttatg tagcatctac ttattaaaaa gcttggtact taggagatta gtggcgttta
     1621 gagttctttt aacaactgtt cgtagtcagc tttttcatcg acaggaagga gcttgtaagt
     1681 taattcgtct gtctcgttgt catgaatgat gtacatctta gcattctctt ctttgaagtt
     1741 accgatttgt aaaatcgtat agcgttcgtc gatcaattcg atatctttga ttgcttttga
     1801 aaaaacttta cggacatggt gcattttctt ttggcggatt tctgcctttt ctgcagcttt
     1861 tgacaaagta aatccgtcat ctaaatattt tttgatcagt tctactttta tgactgtagg
     1921 taaacgatat tttctaggac aattcgcatc agcttcgatc gaacgaatga atcccttttg
     1981 ttcccaatac cttaattgac gtggagacac acctacgata tcacttaatt cactgatgcc
     2041 aacaaccaat tgatcatttt ccaacaaacg tttaagggaa gccccaacca tttttgtcac
     2101 tccttttgtc ctcttgcttc ccttaattat atccgtatta acttatgtgt caagtaggtt
     2161 gacaaatagc gaatttagga ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa
     2221 ggagaaatat gagtaacaac caacccgtag atatttacgg aaaaccatat aatcggacac
     2281 ttttagttgt tgtcttatta attggtacat tttgtaccgt tttgaatcag accttattga
     2341 cgacagcttt tcctacattg atgaaagctt tcgatatttc agcgtctgat gtgcaatggt
     2401 taacgactgg tttcttgcta gtcaacggga tcatgatccc aataacagca tggctgatca
     2461 ataaattcag ttcaagaaat ttatatcttt ctgccatgac gatctttttg atcggaacga
     2521 ttacttgctt tacagcacct aattttagta cattactaat cggtcgtttg atccaagctt
     2581 gcggtgtggg agtatcgatg ccattattgc aaaatatcat gctatctatt ttcccgccag
     2641 aaaaaagagg atcagcaatg gggatgtcag ggatcgttat tggacttgct cctgctttag
     2701 gccctacatt atctggttat atcattgata actaccattg gcgtgacttg tttggtatgg
     2761 tactgccaat cgttgttctt gtcttggttt tagcattctt cctaatgaaa agtgttattc
     2821 cattgtcaaa tcctagcatt gatattttat ccgctatttt atcaacgatt ggattcggta
     2881 gtttacttta cggattttct agtgtaggag atgacggctg gggaagcttg aaagtaattg
     2941 gtttcttagt tgttggtgtg attttcatcg ccttgttctc atggcgtcaa ttacatttgg
     3001 aacatccatt cttggaactt cgtgtattca aatcatctac attcacgatt gcagctgttt
     3061 tagctggtgt aacaaatatg gcgatggtag gggcagaaat ggtcgtacca ttgtatattc
     3121 aaaatattcg tggagaatca gcattccatt ctggcttgat gttgttgccg ggtgcattga
     3181 tcatgggggc tatgatgcct gtgacgggtg cgattttcga taaatacggc gcgaaacgtt
     3241 tagccatcac agggatgttc attttgacag cggcaactgc accatttgct ttcttgacaa
     3301 aaacgactcc agttttatat atcgtgatcc tttatgcgat ccgtatgttt gggatctcca
     3361 tggtcatgat gccggtaacg acttctggta tgaatgcatt gccaaataac ttgatcagtc
     3421 atgggacagc ggtaaataat acattccgtc aaatcgcaag ctcgattggt acagctattt
     3481 tgatcagtgt gttgacgaat gtgacaaaag caaatatccc aagcaaatct gtattggata
     3541 atacgccatt agcttataaa gatcaggcaa tcaatgccac actgtcaggt taccatgccg
     3601 cgtttatcgt agcgatcatc tttgggattc ttggctttat tattgccttt ttattgaaga
     3661 aaaaagaacc tgttctaatg aaagcggggg ataatgcatg attattggag ttttgattgt
     3721 cagcttgctt gcttttgctt taacgaacgt gttcgctaaa aaaacatggc aaaccttttt
     3781 atctttgatt ttcggactaa tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc
     3841 caatcacttt ggaatggaaa aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc
     3901 tgattcccaa ggagcagata tgcttttgta taaagcttta gggaatggaa aagaaaaagt
     3961 gtatctttac cgaacaaatg aaaaacaaga aaaaccaaaa gcaactggta cggataacga
     4021 aacaaataaa gtagaaaaga cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg
     4081 ggaatacaaa aacgacatgt ataaattctg gttcaatatt gctgataaca atcacgaata
     4141 tgacagccga gtgaatacat tcaaaatccc agagacatgg gtagaattaa gtacggacca
     4201 agcagccaag ttagctgaat tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa
     4261 agcttatgtt caagacggta tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc
     4321 agagcaagaa caacgtacga aagccttagc tgctgaattc caacaacaag cattcgcaaa
     4381 attagtgaaa gaagcaaaag gcgaataata ttttgaacac catgta
//
LOCUS       DACEDO010000125         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEDO010000125 DACEDO010000000
VERSION     DACEDO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.129"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWO52_002737"
     CDS             690..1658
                     /locus_tag="IWO52_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0356957.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWO52_002738"
     CDS             1669..2484
                     /locus_tag="IWO52_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ0356958.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWO52_002739"
     CDS             2552..3262
                     /locus_tag="IWO52_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0356959.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWO52_002740"
     CDS             3298..>4425
                     /locus_tag="IWO52_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0356960.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACEDO010000126         4396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEDO010000126 DACEDO010000000
VERSION     DACEDO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.130"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..93
                     /locus_tag="IWO52_002741"
     CDS             <1..93
                     /locus_tag="IWO52_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0356961.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="IWO52_002742"
     CDS             complement(151..1371)
                     /locus_tag="IWO52_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0356962.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..1996)
                     /locus_tag="IWO52_002743"
     CDS             complement(1391..1996)
                     /locus_tag="IWO52_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0356963.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="IWO52_002744"
     CDS             complement(2045..2977)
                     /locus_tag="IWO52_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0356964.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="IWO52_002745"
     CDS             complement(3398..4258)
                     /locus_tag="IWO52_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0356965.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DACEDO010000127         4343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEDO010000127 DACEDO010000000
VERSION     DACEDO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.131"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..978
                     /locus_tag="IWO52_002746"
     CDS             4..978
                     /locus_tag="IWO52_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0356966.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1708..2025
                     /locus_tag="IWO52_002747"
     CDS             1708..2025
                     /locus_tag="IWO52_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356967.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2116..2481)
                     /locus_tag="IWO52_002748"
     CDS             complement(2116..2481)
                     /locus_tag="IWO52_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356968.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2689..3171
                     /locus_tag="IWO52_002749"
     CDS             2689..3171
                     /locus_tag="IWO52_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356969.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3368..4258
                     /gene="pstS"
                     /locus_tag="IWO52_002750"
     CDS             3368..4258
                     /gene="pstS"
                     /locus_tag="IWO52_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0356970.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1507 a          633 c          782 g         1421 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcctgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactgt gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatcttttta
     1621 cgaaaaacat cacaaggaaa aacttaacta tggtattatc taaacataat tttttcggta
     1681 tttaatattt aattaggagg taatattttg cttttttata gtaattttat cttaatagta
     1741 gcgatattat tacttctgaa tatatggatt tttgatagga gtagaaatgc aagtataggt
     1801 tttagaacta aacgaagtct gtcttctaaa aaaaattggg tatactcaca aactattttt
     1861 tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt gaatattata
     1921 gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc aattattaca
     1981 caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa ctagtgatag
     2041 ttttggtgat ataagaaaaa catatatgta taaaaagaag aaagtagctt ctctttgtac
     2101 atatatggtt tgtttttatg gatgccgagc ttttttataa gataaggcta ccgcccataa
     2161 cgacccaagg atttgtggaa ctaagtaaat acctgcaaga agcaagatca gctgggtcgg
     2221 ttttaaagga tgatagaagt aacgccattc agtaaaattg gcgcaaaata aataggaagt
     2281 gacagtagat ataatattgg caatgaataa agatataata cgcctgttcc tttgaaaata
     2341 atatccagca aaaagagata aaaatatcaa tgtgatgaac ccgcttacgg aatgataacg
     2401 aaaatcaagc agtaaggcga aatgcacata aggaacaaaa gataaaagca acggaaggtt
     2461 tttccagcaa gtttttatca tctttcatca ccacctaaca agtgttatta ttttgttagt
     2521 tttattgtaa attgaatcct gcaataatca attacttttt cattatttct agattaaatg
     2581 aataaactct catgccatta tctctaaaaa aaaagttttt ccccctaatt tttttagata
     2641 gaaaattgta tagtatgatg agagaaaaag tagtttgggg gaacgttaat gagaagaaaa
     2701 gcagtaatcg atcggcttta tttatttttt tggttttttc ctatcgtttt gctacctttt
     2761 acatgggtaa atgctgttgt acaaggtgcg gagatattag gcctgatcca aggaagcgga
     2821 tttgtggttt ctaggaattt tccagctttg attgttttct atttagtgat aagtgtaggt
     2881 ctcttattac caaaatctct gatcaatcac tacaattggc agctgcttta cttttcttgc
     2941 ttattagtat ttacatctat gttttctttg ctgcctcgct tgattatcgg ccctaatggt
     3001 ttgagcgaga gtggattgaa ggatttagct tattcctcca tgtatttctt gacgatcaaa
     3061 ccatggttct atttagtaat tgcctcattt attcttgcaa cattttttta cttctggaca
     3121 gaaatcagga taaaaagtga acgatctgta tctataaaaa ataactattg atggtggatc
     3181 atcagtaaaa aaacatatga gctgcgtaaa atgcatgttt ctcatatgct ttttttatta
     3241 ttatgtaaag aatgcgaaaa acgtttacat gatttttaca taacttaact atatttttac
     3301 agcaaccata cgagggattt acagtcgact gataaacttt agttgtaaat aagaaaggag
     3361 caataaaatg aaaaaagtat tgtttactct aggtatgatg ggaattcttt tagccggatg
     3421 cggctcagga tcaggaaata cagatggctc tgccacacaa aataattcaa gtgactcaaa
     3481 tgaacaagta aaaatcgttg cggtaggttc gacagcatta cagccgttag tcgatgcagc
     3541 acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag
     3601 cggaactggt ttgagccaag tcgaagctgg agcagttact atcggaaact ctgatgtatt
     3661 tgcagaagaa agagacggag ttgatgcttc aaaattagtg gatcataaag tagcagtggt
     3721 cgggatggca ccaatcgtca ataaagatac agatgtcaaa gatatcacca aacaggaatt
     3781 gattgatatc tttacaggaa aaatcactaa ctggaaggaa gttggcggga aagatcaaaa
     3841 aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc gcgacattcg aaaaatgggg
     3901 actggatggt gctacccctg tgcagtccca agaacaagat tcatcaggaa cagttcggca
     3961 gctggtaagt caaacaccag gagcaatcag ctatttagct ttctcatatc tcgatgattc
     4021 cacacaagca ctaagtatcg acggtgtaga accaaaagaa gagaatgtag cagataacag
     4081 ctggggaatc tggtcatatg aacatatgta cacgaacgga aaaccttccc ctgaagtcca
     4141 aaaattctta gactacatga tgacagaaga aatccaagaa ggacctgtca aagaattggg
     4201 ctatttacca atcacaatga tggaagtaga acgtgatcac gaaggaaata tcaaataact
     4261 agcagataaa catgccacgg tagtcgttac tattaaggaa aaatcaacta tgtttcccca
     4321 catatacatt tttcctttaa ttg
//
LOCUS       DACEDO010000128         4332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEDO010000128 DACEDO010000000
VERSION     DACEDO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.132"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            63..296
                     /locus_tag="IWO52_002751"
     CDS             63..296
                     /locus_tag="IWO52_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356971.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(455..577)
                     /locus_tag="IWO52_002752"
     CDS             complement(455..577)
                     /locus_tag="IWO52_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0356972.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            767..991
                     /locus_tag="IWO52_002753"
     CDS             767..991
                     /locus_tag="IWO52_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356973.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1434..1640
                     /locus_tag="IWO52_002754"
     CDS             1434..1640
                     /locus_tag="IWO52_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0356974.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1640..1891
                     /locus_tag="IWO52_002755"
     CDS             1640..1891
                     /locus_tag="IWO52_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356975.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            2072..2344
                     /locus_tag="IWO52_002756"
     CDS             2072..2344
                     /locus_tag="IWO52_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356976.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2789..2968
                     /locus_tag="IWO52_002757"
     CDS             2789..2968
                     /locus_tag="IWO52_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356977.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3027..3383
                     /locus_tag="IWO52_002758"
     CDS             3027..3383
                     /locus_tag="IWO52_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356978.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1526 a          675 c          836 g         1295 t
ORIGIN      
        1 gatgatctac cgttttagtt tgagaaagaa ggagtaagca tggcaaaaaa attagtctga
       61 cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg
      121 aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg
      181 tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag
      241 tcacagtagc agcgacagct gttatactta cggctattct accgctttat atgtagagtt
      301 aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt
      361 tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat
      421 ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca
      481 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
      541 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
      601 agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat
      661 agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg
      721 aggtgatcgt gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca
      781 gccattgctt gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc
      841 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
      901 gacaggtcac ttcaataaac gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc
      961 tgatcatttt acgcgatcga tgagcatata aaagaagagc tgcagtccta aatgaggatt
     1021 gtagctcttt ttgttttaca tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac
     1081 aagcgccttc tcaattgaaa gacctttctc tcactccaac cgccaccgcc gttcgagaag
     1141 aaaggaagtg aacgctacgc gttcactaag ataaagggtc ttgcatagca agacaaaata
     1201 gaaaaaattt cctctcacca aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc
     1261 agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg taaatggcac
     1321 gctgcgcgcc cttgtcttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     1381 aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca atcatgcgaa
     1441 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     1501 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat tgtttgtatc
     1561 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt ttaattgatg
     1621 agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
     1681 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagttt tacttattcc
     1741 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
     1801 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacttggaaa cttatgaaaa
     1861 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
     1921 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
     1981 gttcctatac aacgaaacac gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc
     2041 tgtggaagaa atgattgaat aaggtgtcga aatgaatctt attgcgacgt actatcgaac
     2101 actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac tatgcttgga
     2161 agtaggtgtc aaaccaagca ctattaaacc ttctgaatac caagctttag aactaacgta
     2221 tgcgaaattt atcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
     2281 tgaaaatatc aataagtatg gcgcatatta caccatgaaa aaggaagaca acgaaaatga
     2341 ataaggtgat ttatcaacta acaacggatt ccatatcaac gatttgaaag aaaaaaaggt
     2401 caataatcat ttataccgaa aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta
     2461 actaaggaaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
     2521 tgcgaaataa acgggcaaag cccgataact taagagaaag aaggtgatcc atttgaacaa
     2581 gattattccc ttattgttag tactgtttgt tggcgaaata cttatgcgag atagtgcagc
     2641 tgtacagtta aacctcgctg tatttttggt aatactggtg ttttgtttga tcttttggcg
     2701 gtttgtatta gtcctcgtga gtctactatt caagatgatt gcagccatat ttttcatagg
     2761 gttatttcga tgaaaggagt gagtaccaat gccaattgca ataggaaata aacggttacc
     2821 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa
     2881 aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg
     2941 atttgatgta ccagcgctaa aaaagtgacg tcacttttta atttgatcta tataagtgat
     3001 atcactttta ccaaggaggg aattaaatgt taaaaactaa aaagaattcg tacaaattaa
     3061 agcttagtat actatctatt atttgtcttt taggcatgat aattagtggt catttgtata
     3121 gagtggcata tacgcagttt attgagtaca aggttcactc tttaggatta gctggggatc
     3181 gatacgttga atatgtccat tattcattaa aatgttcttc atatattctt ctttggctta
     3241 ttttttcatt gtttgaattg ctaaatttaa tagcaatatc ttcatatcac ttagataagc
     3301 taaataccaa atctagccgt tataacgaag tgtttaagga aaatgtgaag ttagaaagtg
     3361 acttaaacaa gctaaaaaaa taaaaagaag aaaacataag ttaactaaaa ttattttatt
     3421 aagaatgatg gaatataagg ataaggttac caacataacg atcattactt ctctgatatg
     3481 ctatggactt ctatcaatca gtattttgtc tatagattat ggagtaacta tactgcaagc
     3541 cttagttttg gctatgccta tgtatctatc tttgtttatc atgcatgaaa tatataagct
     3601 atttgtttta tcaaaaaatg tagaaatacg ggtaaatatt ttggtgaata gttatcaaaa
     3661 agaactcaaa gaaagaagac ggtggcggta tttattgaaa ggatgaaaaa gtaggatttg
     3721 acatactggt attcagaaag tgccgtcact tttaaaacaa tggaaaaggt ggagataaga
     3781 tgaagaaaaa acaaactaaa ctatcattag cagatattta acacagttaa ccgcaactga
     3841 agatggtgtt gttgaatttg aacaatctga aattgcggta gtagatgatc gttaccttaa
     3901 aatgccttac ttctttgacc aagcaaaggt catttgcctt tgtcactacg atggggtacg
     3961 tgattttagg cgttaaaaag tagtgattat atcaacttta cgagtaaata taaatttaga
     4021 catgaaactg aattatccaa tggaaaatca tggtttataa tctagaaaaa ttatagtaat
     4081 gattggatta tatatattct ttcgtatttt cgtacaactg tagcttgtca aaattgagaa
     4141 atttaaccga ttaggtactt tacatcgtta tttgggtaat ttctaaaaca tgaagcttac
     4201 caacgaataa actgagacta tcttattaat tgatcaatat aagaaaatat ctttgaggag
     4261 atgatgtact atgttttcgt ctatccacga ttggttaggt ggtcttttcg gcggtggagg
     4321 taaaggtttt ta
//
LOCUS       DACEDO010000129         4174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEDO010000129 DACEDO010000000
VERSION     DACEDO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.133"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            147..1094
                     /locus_tag="IWO52_002759"
     CDS             147..1094
                     /locus_tag="IWO52_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0356979.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1106..2026
                     /locus_tag="IWO52_002760"
     CDS             1106..2026
                     /locus_tag="IWO52_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0356980.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2067..3524
                     /locus_tag="IWO52_002761"
     CDS             2067..3524
                     /locus_tag="IWO52_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0356981.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3869..>4174
                     /locus_tag="IWO52_002762"
     CDS             3869..>4174
                     /locus_tag="IWO52_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0356982.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     "
BASE COUNT         1315 a          711 c          834 g         1314 t
ORIGIN      
        1 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
       61 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
      121 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
      181 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
      241 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
      301 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
      361 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
      421 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      481 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      541 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      601 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      661 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      721 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      781 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      841 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      901 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      961 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
     1021 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
     1081 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
     1141 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
     1201 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
     1261 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
     1321 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1381 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1441 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
     1501 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1561 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1621 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1681 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1741 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1801 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1861 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1921 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1981 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     2041 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     2101 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     2161 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     2221 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     2281 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     2341 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2401 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2461 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2521 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2581 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2641 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2701 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2761 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2821 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2881 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2941 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     3001 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     3061 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     3121 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     3181 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     3241 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     3301 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3361 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3421 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3481 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3541 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3601 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3661 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3721 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3781 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3841 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3901 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3961 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     4021 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     4081 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     4141 atatttatct gttcaaatca aagggctcat tttt
//
LOCUS       DACEDO010000130         4041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEDO010000130 DACEDO010000000
VERSION     DACEDO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.134"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IWO52_002763"
     CDS             179..775
                     /locus_tag="IWO52_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0356983.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IWO52_002764"
     CDS             899..2698
                     /locus_tag="IWO52_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0356984.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IWO52_002765"
     CDS             complement(2976..3188)
                     /locus_tag="IWO52_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356985.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1188 a          689 c          712 g         1452 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DACEDO010000131         3925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEDO010000131 DACEDO010000000
VERSION     DACEDO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.135"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(573..1130)
                     /locus_tag="IWO52_002766"
     CDS             complement(573..1130)
                     /locus_tag="IWO52_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356986.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IWO52_002767"
     CDS             complement(1309..1920)
                     /locus_tag="IWO52_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356987.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IWO52_002768"
     CDS             complement(2033..2290)
                     /locus_tag="IWO52_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356988.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IWO52_002769"
     CDS             complement(2887..3165)
                     /locus_tag="IWO52_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356989.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IWO52_002770"
     CDS             complement(3199..3450)
                     /locus_tag="IWO52_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356990.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DACEDO010000132         3904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEDO010000132 DACEDO010000000
VERSION     DACEDO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.136"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(287..508)
                     /locus_tag="IWO52_002771"
     CDS             complement(287..508)
                     /locus_tag="IWO52_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356991.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(606..851)
                     /locus_tag="IWO52_002772"
     CDS             complement(606..851)
                     /locus_tag="IWO52_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0356992.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1091..1705
                     /locus_tag="IWO52_002773"
                     /pseudo
     CDS             1091..1705
                     /locus_tag="IWO52_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2075..3076)
                     /locus_tag="IWO52_002774"
     CDS             complement(2075..3076)
                     /locus_tag="IWO52_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAQ0356993.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3235..3501)
                     /locus_tag="IWO52_002775"
     CDS             complement(3235..3501)
                     /locus_tag="IWO52_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0356994.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3491..3847)
                     /locus_tag="IWO52_002776"
     CDS             complement(3491..3847)
                     /locus_tag="IWO52_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0356995.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1239 a          704 c          517 g         1444 t
ORIGIN      
        1 actaaaatag attttttgga aaactttgca acagaaccgt ttttactata tacttcttcc
       61 cagaacttgt ccgtctcctt caaattatct ctacctctcg agttcattgg gtggtttgcc
      121 catttattaa acatggaatc acctttctca tactcatata tttatgtcct atttcaatta
      181 tatttatata tgcttaaata aatttgtatc ctgaaattta ttgtaatatg aagctgtatt
      241 ttacatatag taaccaaaga gttaataaaa taaaaagagc gaaccattat agatcagctc
      301 ttttttcatt gctgttttta atcttatcta gaatcacttc aaatgtttca aaggtattta
      361 catctaactg acataaccaa agcaagtact tcttttcttt gtaagtccaa tcaatattac
      421 ggtaagagga cattagatta tggtaccttg ctctttgttc aactgaagcc aattcccagc
      481 gtttttcaaa tattttttct tcttccatta ttgatccctc gcaagacgct acctcaaact
      541 taaataatgg gcagcgaaag tctaattcct tagatctaag tatagcaaac aaatatgaaa
      601 aaaacttaga aggtagtaaa agttcttccg tcttttgctt tgcttctatt cttaatgtgt
      661 ttcgttatcg gtgccctaca aagattttca ttaattacac actcatttat tgttgtgtat
      721 tttctgacat ttctctcttg atcaattatt ttaattccat gggcatcctg atagttcata
      781 ggagaggagc ggctatcaga aatagttgca cctatctctt gaacttcttt tacaacttct
      841 ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata atctataata tttatctttc
      901 cttttcaatc taagaaatcg tcaccccatt ctttttcttc tattataaac tatttaactt
      961 ttagaattca aaaaccgttt gtctatttag agtttaccct aaatagacaa aaatataaca
     1021 ttgaaaaatt tagagttcta acgtatgatt agggtgtatt ataaacgtta tagaaaagta
     1081 gggaataaat ttgaaaattg cttatattag agtttcatcc atcgatcaaa atgaacaacg
     1141 tcaaattgaa gaaatgaaaa agtttggcgc tgaaaaaatt tttatcgaaa aacaatctgg
     1201 agcgacgatt agtcatcggc cgattttcca ggaagcttta gattttgtca gagagcaaga
     1261 tatttttatt gtagaagcca ttgatcgact tggacgcaat tatgacgaga ttatcgattc
     1321 agtgaattat ctaaagaaga agaatgtgcg acttattatc accagtttac cgatcatggc
     1381 tgaagcaatt ggaaatcctc tcctagataa attcatcaaa gacttaatca tttaaatctc
     1441 agccatgatt gctgaacaag aacgtactga atcgaaacga cgccaggcac aaggaattaa
     1501 aatcgctaaa gcaaacggcg tctataaagg tcgtcctaag ttatacagtg cagacaccaa
     1561 agatcctcaa cgtcgccttg tctataggag tatcgttcag gatcttgaga atggggttgc
     1621 tatctcaaag attgcaacag actacaatgt gactaggcaa acaatttacc gaataaaaaa
     1681 agaaatcgac caactaattg tatgatttct tttttgaatc acgtaattat taattagctt
     1741 tgtcatcaat acagacaatc attttagatt catctagcta agttgattac atttatgtta
     1801 aaaagaattt agttaacata aatataattg atgcataaat cgtccttatt taaaggcatt
     1861 taacattgac aacttagtat aatatatctt atgtaaacta ataagatttg aattttattt
     1921 tatacacact cgataataga tcaattttat ggtcccccgc atttacgatt gcacaagaga
     1981 aacataaagt cgacctatta gcatttcttt tcaattcaaa cattaaaaaa gtttatacct
     2041 ctgctactaa aaaatgaaat tatctattta aaatttaaaa cggtaaatct gattcatcca
     2101 aattggaaaa tggatcagcc tgtattggag ctttgtattt ctccataata acagatatct
     2161 ttttttcatt ttttaaaaaa taccttttaa atttcttata gtcctctgaa atcaaaatat
     2221 agcgtgatgg atactcatca aaagaagaat cgtattcttc ctttaaaatt cccgatttac
     2281 ttaatttttc tagtacatca aatactctat cactcaagta actccttaaa ttgttatcac
     2341 tttcccattg aactactttt ctataagttg aattatcagt tgaaaagata gaattttcat
     2401 ccataaacgc atatattagc agcaatcttt catcattgtc aaattctatt ggatcaaaaa
     2461 tatctaagtt gtcatcaaaa tctttatcag ttccggtaac ttttttgtta attactgaag
     2521 gtgtcttgta agaaatagaa tctaaatcaa agcgttttga ttctaaatta ttttgtgctt
     2581 tctcaattaa ataattcact ttttttgtga gaaaccttct gatattgtct ttgctatatg
     2641 ttccataaaa acccttatct tctaaagagt ccctgtactt cttaacttct atatactctt
     2701 cagccaaaac ttgtggaact ggttcatcta agaaaaaaac ccctacctcg cctttgctat
     2761 ttttataaaa gtaatcaatt tctgctatag ttcccgatct ataattatca actggagaac
     2821 ccaacttatg gccaaaaatc gctaataata tatcactatt ttctacaatt tgtttattaa
     2881 taacactttg cccagagcta ttatttatat aagtaggaat cgcattcgtt tcccatcgaa
     2941 ttggcatcaa aattactttt gtattatctg tattttctat attccatcta tatatggttt
     3001 cttctacttc attcctcttt gctgctatat cacctggtga tgctataaat acttttatta
     3061 gatttatggt tttcatttat tgtacctttc tctttatgtc tctttaaagt aatttttgta
     3121 taaaatttat tgacttttac actataggac aacttttatc tatttagtac aaaaaggccc
     3181 actaaactaa ttgattagtt aggagcctat tacaggtata ttttcaactc gcttttaagc
     3241 aaattctact accagctttt tgcctaatgc cctagcgata tcatcaagtt tatcgaaaga
     3301 tacgttatcc cctctctcaa ttcgagcaat agtagattga gttgtatgtg ctttttgtgc
     3361 aagctccgct tgagttaggc ctgcttcctc ccgtgcatga taaagagcaa ccgcagtttc
     3421 gagttttctt ccttcacggt catatgcttc tgcaaaatct ttgtcttttt gcttttcttc
     3481 aatcaatttt ttaatcatca tttgtgaacc actcctctct aagttctttt gcatgtttaa
     3541 gttcacttat tggtgttttc tccgtttttt tagtaaatcc atgggtaatg acataccttc
     3601 cattgaccac atggaaatag atagctcttt ggatgtttga tcctacctta cttcttaatt
     3661 cgtacaaatt agtgtctaat tttttcaccc atttcaaccg ttgagctact agcatcccat
     3721 tcttttctgt ttcttctatt gtacgtaaaa gcttcgcact atcttttttt ggtaatgttt
     3781 ccaaccattc taaaaattca tcatgaccat taggtctttt aaatgtttca aaagatattt
     3841 tttccatacc caattatatc atatattgat tttatattat agtatataac ataaaaattc
     3901 gaat
//
LOCUS       DACEDO010000133         3645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEDO010000133 DACEDO010000000
VERSION     DACEDO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.138"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..312
                     /locus_tag="IWO52_002777"
     CDS             <1..312
                     /locus_tag="IWO52_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0356996.1"
                     /translation="FLAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISV
                     FSSFSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESY
                     E"
     gene            305..2029
                     /locus_tag="IWO52_002778"
     CDS             305..2029
                     /locus_tag="IWO52_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0356997.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            2026..3477
                     /locus_tag="IWO52_002779"
     CDS             2026..3477
                     /locus_tag="IWO52_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0356998.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         1324 a          588 c          720 g         1013 t
ORIGIN      
        1 tttttagcta ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa
       61 tacagtatga ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt
      121 tttatttcgg ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata
      181 attttaggcg tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg
      241 acggtttgga atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa
      301 agttatgaat aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg
      361 gaaatacgcg gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca
      421 tacttgggaa caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac
      481 aagcagaatg ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg
      541 gaattccgaa aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct
      601 gatgtatgta tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat
      661 caaagatctc tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact
      721 accggagtat tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc
      781 aaaattacta gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg
      841 aagtattttt attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc
      901 attcaatgga ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca
      961 tgataataaa atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct
     1021 gaagaaaaat tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca
     1081 agaactgtct gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga
     1141 aacgggaaaa aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct
     1201 tatctttctt tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc
     1261 catggatcaa acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata
     1321 tgaactgaag gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt
     1381 agaagacatc tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc
     1441 acagatcaat ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag
     1501 cgaaggaagt gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa
     1561 tacgaatcaa gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt
     1621 ctatttgtat caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga
     1681 tttggaaaag atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa
     1741 acacggtatt gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg
     1801 caaacgggta aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa
     1861 acagattgaa gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca
     1921 aaacgtgaat gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa
     1981 taatgagaca ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa
     2041 gtgttattgg tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg
     2101 caaagtttag gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta
     2161 cttgaacagc gacagattga tttagtagta acggatgtga caatgccaga aataaatgga
     2221 ctggaattta ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca
     2281 ggataccaag aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg
     2341 atgaaacccg tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta
     2401 gatcagcaaa atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa
     2461 tggttgaatg aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag
     2521 aagcagcata ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga
     2581 tggttgcaag aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg
     2641 atcacgttgt tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac
     2701 tttcctgatc aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc
     2761 cctgaaacat tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa
     2821 gacgagcatg ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca
     2881 agtttcagac gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat
     2941 ttttttgaac agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt
     3001 ttactgttta tggagattca acgggaattg attgatctgg aagatgagga atatttacaa
     3061 ggaatcgaaa aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca
     3121 ttcatcaaaa agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta
     3181 atcctgcacc agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat
     3241 ttaaatgtca tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct
     3301 tatttgaacc atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac
     3361 atcaatgaga tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc
     3421 aagaaaatcg tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag
     3481 aaaaagccaa tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg
     3541 ttcttattac ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag
     3601 ccagtttctc ggtattaagc caaatttatg caaaaatgtg gaaag
//
LOCUS       DACEDO010000134         3416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEDO010000134 DACEDO010000000
VERSION     DACEDO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.139"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(22..306)
                     /locus_tag="IWO52_002780"
     CDS             complement(22..306)
                     /locus_tag="IWO52_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0356999.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(296..1030)
                     /locus_tag="IWO52_002781"
     CDS             complement(296..1030)
                     /locus_tag="IWO52_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0357000.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            1159..1782
                     /locus_tag="IWO52_002782"
     CDS             1159..1782
                     /locus_tag="IWO52_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0357001.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            1974..3095
                     /locus_tag="IWO52_002783"
     CDS             1974..3095
                     /locus_tag="IWO52_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0357002.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            3102..>3416
                     /locus_tag="IWO52_002784"
     CDS             3102..>3416
                     /locus_tag="IWO52_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0357003.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGN"
BASE COUNT         1085 a          609 c          726 g          996 t
ORIGIN      
        1 tatttaccgt gtttttaaca ttcatataga ttcaggaagc aaaacggttg agtgtgtctg
       61 taaataacgt tctttttgtt ttcgggtgag ttttttgaat gcagcttctg cttttgttgc
      121 ttcgcttctt gaagaaaata cttctgcatg gatcatctta acaggtcttc ttttttgaag
      181 acgagtatat ttagcaccag taccattgtt atgttcctct aatcgtctag aaagatcggt
      241 tgtatatcca ccataaaatg tctgatcttt acagaataat acgtaaaaat aatgttcatt
      301 tgccatagag catctcacga atctctgcag tgtattctcc atccatattg taagtgatca
      361 atggtggagc gactttgaat ccgtctaatt tgccttcttt gattccttca actaacaaaa
      421 tatttgcctc ttttccaggt tttgggtaaa caaattggat ccttttaggt gcaattcgat
      481 agtcttccat gacctgaatg atttctaaga accgatcagg acgatggacc atagtaaagc
      541 gtccatttgt ttttagtagc ttacttgaaa tatcgatcac ttcttctaac gtcgtatgaa
      601 tctcatgacg ggcgattgcc aggtgtttat tagggttcgt tttatttgtt ggcagccctt
      661 tgaaataagg tggattacag acgactaagt cacaagaatt atgtcgaacg acagataagc
      721 tgtctttcag atcgatggtg tggacagtca tttgttcatt taaatggtta agcgcgatac
      781 tacgtcttgc catgtctgct agacgaggtt gaagttcgat agcgtcgata gccgcctggg
      841 ttcgtttact taaaaaaagt ccaactgcgc catttcctgc acaaaggtca acaatcttcc
      901 cttttttagg tacacgggaa aagtgagcaa gcagcacagc atctaaagaa aaagaaaaca
      961 ctttggaact ttggataatc ttgatatctt ctgcgtataa ttggtcgatt cgttcgtctt
     1021 cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg tctgtattat actattttta
     1081 ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt atgcatacta tgaccataag
     1141 aagggaaagg aagagactat gttttatcga tttatgcgcg gggtagttcg agtactatta
     1201 gccttagtta atggaaatgc ccgctatgag aataaagaag ttttaccgca agatgagaat
     1261 tacatactag ttgctcccca tcgtacttgg tgggacccat tatatctggc ggtagctgca
     1321 gcacccaagg aattcagctt tatggcaaaa gaagaactgt tcaaaaatcc agttctgcga
     1381 tttatattaa ctcgatccaa tgcttttcca gttaaaagag aaaatccagg accaagcgtc
     1441 attaaaacac ctgtaaagat tttgaaatcg acaaatttga gtttgatcat gtttcccagt
     1501 gggacaagac atgctacaga acttaaaggc ggaatggcat tgatttccag aatgtctaaa
     1561 gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac ttggggatct gttcaagcgt
     1621 cagagagtga cagtgcgttt cggcgaacca atcgacttat cagatatcaa gaaaacggat
     1681 aaagaaggta tggcggagat cgaacgacga acacaagcag cttttgacaa attggacaaa
     1741 gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat aaaatgataa gtttatgcgt
     1801 gggcggtctt ttgaatctac tgtcctgttc agttcagact ttttcaatga aaacgaggga
     1861 gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta aaatagaaga caggtgtcat
     1921 gtaatggatc aaagtgttat aataattaga aggacatttt ttgaggtgat tgaatgcgtt
     1981 ttttacatac agccgattgg catatcggaa aaaaactaca aggttatgac ttgattgaag
     2041 accagcgtca tgtactatct tctatactag atatcgcgaa agaagaaaaa gtagaggcaa
     2101 tcgtgattgc cggagactta tatgatagga gtgtcccagc tgttgatgcc gtagaattat
     2161 acaatgaatt gatggtcgat tggaatttaa aggaaaagat cccgattttt gctatatcag
     2221 gaaatcacga tagcagtacg agactttcag cgggtacgcc atggttcagt tattcgaact
     2281 attttttgcg aacacagcta gcagaagcat ttgaaccagt cgagtttaaa aacgtacaat
     2341 ttttctttct tccttacttt gaaccaattg cagcgagatt atattttgaa gatgacaata
     2401 tccgaacgat caagcaagcg gtagccaagg tgatagataa aatgcaggag ctatttgatt
     2461 cagaaaaaaa acaagtattg attagccatt tttttgtaat gggaagtgta aaatctgatt
     2521 cggaaacgaa aatcgaagta ggcgggttgg attcgatccc tggtgaacta cttacaccgt
     2581 ttgattatgt agcactagga catttgcata atcaagctgc tttgagacag gataatgctc
     2641 gctatagcgg ttccccattg aaattctctt tatctgagat caaccaaaaa aaaggcgtat
     2701 ggatcgttga cctgacaaca ggcctggagt tgaattttag ggagatcgaa cccctgcatg
     2761 atattgaaga aataacagcg agtttcaaag aattgctgga tcctgccttt tatcagtccg
     2821 tcaatcggga gaattacatc tatatctatc tcactgatag aacgatcatc ccgaatatga
     2881 tgaaccagct tcggcaaata tatcctcgta ttttggggat tgaacgggca aacggtcgag
     2941 cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa agatcctcaa aaattagcag
     3001 aagcattttt tagtgaaatg acggaagagc agctaactgc aaaccaagaa aaatggctcg
     3061 gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag aatgaaaccg aaaaaattac
     3121 aacttaaaaa ttttggtcct tttatcaatg agaccgtaga tttttcacgt ttgacagaag
     3181 caccgctttt tttgatcagc gggaagacag gagctggcaa aacgacgatt tttgatggga
     3241 tcaccttcgc actttttggt gaaacttccg gaagacttcg ctctggaaaa gagatgcgtt
     3301 ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt ttcttttgaa catcagcaga
     3361 tgagctatga aatcgaacga aaaccagaac aagttttagc aaaacgaaaa ggaaac
//
LOCUS       DACEDO010000135         3399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEDO010000135 DACEDO010000000
VERSION     DACEDO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.140"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..277
                     /locus_tag="IWO52_002785"
     CDS             <1..277
                     /locus_tag="IWO52_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ0357004.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="IWO52_002786"
     CDS             420..641
                     /locus_tag="IWO52_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0357005.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="IWO52_002787"
     CDS             657..1499
                     /locus_tag="IWO52_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357006.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="IWO52_002788"
     CDS             1562..3364
                     /locus_tag="IWO52_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ0357007.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1230 a          469 c          702 g          998 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaata
//
LOCUS       DACEDO010000136         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEDO010000136 DACEDO010000000
VERSION     DACEDO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.142"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(106..1053)
                     /locus_tag="IWO52_002789"
     CDS             complement(106..1053)
                     /locus_tag="IWO52_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0357008.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IWO52_002790"
     CDS             1299..1763
                     /locus_tag="IWO52_002790"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0357009.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IWO52_002791"
     CDS             complement(1825..2868)
                     /locus_tag="IWO52_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0357010.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          996 a          597 c          577 g          843 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gct
//
LOCUS       DACEDO010000137         2981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEDO010000137 DACEDO010000000
VERSION     DACEDO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.143"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(20..955)
                     /gene="murB"
                     /locus_tag="IWO52_002792"
     CDS             complement(20..955)
                     /gene="murB"
                     /locus_tag="IWO52_002792"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0357011.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1028..1948)
                     /locus_tag="IWO52_002793"
     CDS             complement(1028..1948)
                     /locus_tag="IWO52_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0357012.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2061..2813
                     /gene="xth"
                     /locus_tag="IWO52_002794"
     CDS             2061..2813
                     /gene="xth"
                     /locus_tag="IWO52_002794"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0357013.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          841 a          602 c          517 g         1021 t
ORIGIN      
        1 ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat attgcttgtt
       61 tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct ttgattactt
      121 cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg atattgacga
      181 tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct tgcaatcctg
      241 cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca cttccgcatg
      301 aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc attcttgctt
      361 tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg atagcacgta
      421 attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc aaagttttca
      481 atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc tctccgccat
      541 acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg caagcaaatt
      601 caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt gaggcacctg
      661 cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc agcatgatga
      721 ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct aataccatcc
      781 aaggtatatc atggattcgg caataatcga tgatttgctt tacttcttct tgttttttcg
      841 ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa cgtatagttc atcaatggtt
      901 catcaaacaa tatagtaatc gtggctaagc tttcaactat ttcttcttta ttcactgagg
      961 attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac aatcttgcca
     1021 gtcttgttca aagttgatga cggtagtgat ctttaagcac ttcctcacat aattcatgac
     1081 ttcttacaac ttctttttgt ttaagcaatt ctgtttccga tttacctaaa cggatctgag
     1141 aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt gtccagtcgc
     1201 caaattcttt cacttttttt ccatcagcat cttgaatgac aagctgggtc agatcgttta
     1261 cttcataagt accattttta cttgttactt gatatttttc tatattagcg ccacttgcta
     1321 aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg gcatattcca
     1381 aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca tctaataaat
     1441 acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaactct gtcgtttctt
     1501 ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctgggata cgtttcaatt
     1561 cttctaccat aggagcaaag cgccggttga aaccgatcat caaaattatt tggttcttat
     1621 cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt ttgtcaatat
     1681 aaacatggat accattttct agacattttt tggctaattc ataatgtact tttgttgctg
     1741 cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt tcatatagat
     1801 gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt gttgcaaaat
     1861 aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt tctgcgatat
     1921 tccctacacc gattacacca atcttcatct actttcctcc tggatcattt agttactttc
     1981 atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta aggaaattaa
     2041 ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg cttcgtgcca
     2101 tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt ttctgtctgc
     2161 aggaaacgaa actccaagca ggacaaattg atttagatct tccaggttat tatcaatatt
     2221 ggaattacgc ggaacgaaaa ggctattccg gcacagcgat ttttgctaaa aaaccagcat
     2281 tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt ctgattactt
     2341 tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa agcgaattaa
     2401 aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg gaaaatctaa
     2461 aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgctcatcaa aagattgatt
     2521 tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac tccagaagaa agagcggctc
     2581 tttctcgact gttggataac gggtttatcg atacattccg ttatttttat ccaacccagg
     2641 aaggtgtcta ctcttggtgg aactaccgat tcaattcaag gaaaaacaat gctggttggc
     2701 ggatcgatta ctttttgacg agcaaagatt tagagccagg actagctgat gcaaagatcc
     2761 atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa taaagaggtt
     2821 gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     2881 tttgcataaa tttggcataa taccgagaaa ctggcttttg aacactgttt atcgagagat
     2941 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca a
//
LOCUS       DACEDO010000138         2958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEDO010000138 DACEDO010000000
VERSION     DACEDO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.144"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IWO52_002795"
     CDS             complement(<1..302)
                     /locus_tag="IWO52_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0357014.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            661..915
                     /locus_tag="IWO52_002796"
     CDS             661..915
                     /locus_tag="IWO52_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0357015.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            916..1233
                     /locus_tag="IWO52_002797"
     CDS             916..1233
                     /locus_tag="IWO52_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0357016.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1792..2727
                     /locus_tag="IWO52_002798"
     CDS             1792..2727
                     /locus_tag="IWO52_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357017.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2821..>2958
                     /locus_tag="IWO52_002799"
     CDS             2821..>2958
                     /locus_tag="IWO52_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0357018.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT         1065 a          426 c          540 g          927 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatg gatgttaacg
      481 gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt tttcagcgtt
      541 ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga aacaccgttg
      601 gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga gtgtgataac
      661 atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat ccttccaagc
      721 tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa cttattcata
      781 ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga aagtttccaa
      841 gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt cggaaaatac
      901 ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt tcagaccagc
      961 cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc cagcataaaa
     1021 agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc aactatgcat
     1081 gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt gatcggcaaa
     1141 agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt aggacgtatt
     1201 ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag tcgctcatcc
     1261 tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt actcgaagat
     1321 tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa ggtctaaaga
     1381 tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat cgcttggtga
     1441 ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat tcccaaaaat
     1501 ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat tgacgtaact
     1561 tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa gaggaagtcc
     1621 aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat acatccctgc
     1681 tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa tgggataaaa
     1741 tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat agtgaacata
     1801 cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac tttcaaaaga
     1861 ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata caagtttaat
     1921 tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc tgagagtagg
     1981 ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat atatacgttc
     2041 cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga atatctcaag
     2101 gggaatagct atctcaagat tcgattttca catatattaa ttttactacc atatttgaat
     2161 gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa tcaaggaaat
     2221 atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg ttacggaatt
     2281 ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg ttcattatta
     2341 atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga aaacatgcgt
     2401 ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt tttgaaaatt
     2461 caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc acattttggt
     2521 gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa aatcaatgct
     2581 ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat aacagatatc
     2641 tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact tgttaataaa
     2701 aataaaggaa cccgtggtgc tagttaattt tttcctagag aaaaatgtct aaacatgttg
     2761 gtaaatcaac aaaaaaacgc atagagtagt ctcaaaactc ccaagaaatg aggactacat
     2821 atgcgttcat caaaatatac tgaacattat acagatactt ttataacatt caaggaaatt
     2881 atgagaactg tttctaacat ataccacaat tgtgtaccag ataagattaa aaaccgaaga
     2941 aatactgatc aactgaag
//
LOCUS       DACEDO010000139         2821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEDO010000139 DACEDO010000000
VERSION     DACEDO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.145"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            188..511
                     /locus_tag="IWO52_002800"
     CDS             188..511
                     /locus_tag="IWO52_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357019.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            675..929
                     /locus_tag="IWO52_002801"
     CDS             675..929
                     /locus_tag="IWO52_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357020.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            999..1244
                     /locus_tag="IWO52_002802"
     CDS             999..1244
                     /locus_tag="IWO52_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0357021.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1320..1685
                     /locus_tag="IWO52_002803"
     CDS             1320..1685
                     /locus_tag="IWO52_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357022.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1746..2309)
                     /locus_tag="IWO52_002804"
     CDS             complement(1746..2309)
                     /locus_tag="IWO52_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357023.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          835 a          493 c          432 g         1061 t
ORIGIN      
        1 catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca
       61 caagtttcaa aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata
      121 actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt
      181 attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc
      241 ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat
      301 gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa
      361 aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat
      421 catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt
      481 agatatcaat ccattgattt tttctcgata gataagacac attcagcgga acagttcctg
      541 cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt
      601 tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt
      661 gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac
      721 tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt
      781 tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg
      841 atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt
      901 atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata
      961 gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt
     1021 tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag
     1081 tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag
     1141 aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg
     1201 attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg
     1261 tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta
     1321 tgcgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac
     1381 atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt
     1441 atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa
     1501 tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat
     1561 catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac
     1621 acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta
     1681 cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc
     1741 ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta
     1801 aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg
     1861 acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt
     1921 cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat
     1981 cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag
     2041 tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc
     2101 agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt
     2161 aaaaatagtt tttttatcat aaggttcccc ccctgaaaat agactaaaat aataacgctt
     2221 tctttttttg ttttcagacg aaaattaata atttctaaat attctttaaa aaagcttata
     2281 actattatgc cttttttaat atataacaca gttattttta aaaataagaa tgctaaatca
     2341 ctctacaata acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag
     2401 atgaaagatc aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact
     2461 actttttcct ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct
     2521 ttcaatgctt gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc
     2581 tagaacatat tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa
     2641 tttcgatagg ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc
     2701 aagataggaa tctttttatc aacatcactt tattcgtatt ttcctctacc ataaaagctt
     2761 cacgctccac aaaattgttt tatacactac tattcagccc attcaacgac caatgaattt
     2821 t
//
LOCUS       DACEDO010000140         2532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEDO010000140 DACEDO010000000
VERSION     DACEDO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.147"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..1150
                     /locus_tag="IWO52_002805"
                     /pseudo
     CDS             6..1150
                     /locus_tag="IWO52_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="IWO52_002806"
     CDS             1321..1779
                     /locus_tag="IWO52_002806"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0357024.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IWO52_002807"
     CDS             1938..2408
                     /locus_tag="IWO52_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0357025.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          840 a          483 c          477 g          732 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc aacccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tg
//
LOCUS       DACEDO010000141         2484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEDO010000141 DACEDO010000000
VERSION     DACEDO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.148"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1015..1305)
                     /locus_tag="IWO52_002808"
     CDS             complement(1015..1305)
                     /locus_tag="IWO52_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357026.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2204)
                     /locus_tag="IWO52_002809"
                     /pseudo
     CDS             complement(1513..2204)
                     /locus_tag="IWO52_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          459 c          348 g          943 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgatttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacg
//
LOCUS       DACEDO010000142         2405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEDO010000142 DACEDO010000000
VERSION     DACEDO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.149"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..102)
                     /locus_tag="IWO52_002810"
     CDS             complement(<1..102)
                     /locus_tag="IWO52_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0357027.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(176..457)
                     /locus_tag="IWO52_002811"
                     /pseudo
     CDS             complement(176..457)
                     /locus_tag="IWO52_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            919..2301
                     /locus_tag="IWO52_002812"
                     /pseudo
     CDS             919..2301
                     /locus_tag="IWO52_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          874 a          412 c          400 g          719 t
ORIGIN      
        1 caattcctga acttcacgat agcttagatt gtaacgcagg tagtaaccaa cagcgacaat
       61 aatgacgtct tttttgaatt gtttgccttt aaaatgattc attactctgt cctctctgtc
      121 ttttttctca attttacact aaaatagatt ttttggaaaa ctttgcaaca gaaccgaaat
      181 gagtatagta tgaattagta atttggtttt catctaagtt tagctttgat ttactaaaat
      241 tgatatctcg atacatgttc aatacatcta atacaaatcg agaatcttct ttcggaaatg
      301 gctcagaaag atcactccaa atttcatcat cataatgata ctcgtaacct tctctcaata
      361 tttcgatttt tctttcataa taagatctgt tttggtcctc aaatttagcc aataactcaa
      421 attgatttac tagtgacaat ctctgaaaac tgtccattaa atgtttcttc ctatcttgtt
      481 cattaacaaa aaattataag cccttacaac gaatattata tattaaattt aattgtaggt
      541 gaacacgaat tttataaata tttatggtgt taatgacaaa tgatatccga ctcctcttca
      601 tacgttctgg actatttgct aatagattaa aatttcagca tctatttctg accataaata
      661 aatgagctgc cgaaaagctg ccaaatcaga cttgaaaata aaggatcaaa aacttttaag
      721 tttctgaccc cgtaactcac acttcaaaat cttacttaaa aaggagtctg aaagaaaaaa
      781 agagtattca aaattttttt ctaactagct acatcacatc tatgcattga cactcaaaga
      841 accagaataa gtcatcagaa agcaaggggg tgcggacata atcctggacg aaaaaataaa
      901 cttaggagga atgtccgtgt gttttcccac aaagagcgtg taagggcaat tcagttattt
      961 ttaaaatacg attgctctta tgctgcaacc attcgagaat tagggtatcc gtcgattggt
     1021 gcactacgga aatggtataa agagtatttg gaatccggag agctccatca ggagcaccga
     1081 aaaaaatcaa agtattctga agaacaaaaa cgaatagcgg tgaatcatta ttttgaatat
     1141 ggtcaatgtt atgcacgaac cattcgcatg cttggttatc ctaatagaga gtccttgcgg
     1201 cagcggtgtg aagaacttgc accaggagct agaaaactcc gaaaaagtgc ggtaaagtta
     1261 acacaagatc aaaaagaagc tgtgttgaaa aaattctata aaccgcaaac taataggaaa
     1321 aatttagccg aatctggagg aattagtaga gttactctat atcaatggaa aaacacggtt
     1381 cttggaaagg attttccatt gtgtatgact ttaaaaaatc aggaaaaaca aaaagagcta
     1441 cttttaaagg aagtagaaga cttacaaaaa caagtacacc aattacagct cgaaaaagca
     1501 ttactcgaag gagcggctga actgctaaaa aaagaaaaag gcgtcaatct tctttgtcta
     1561 tcaaatcaag aaaaaacgat attgattgat gccttacgaa atcagttcac cttgaaagag
     1621 cttcttcaac agctccagtt tcctaaaagc agctattttt atcaaaaaca agcacttgag
     1681 aaaccagata agtattacaa agaacgtcaa attaattatc acgattttca atcataactt
     1741 ctgtgcgtat ggttaccgta gaattcacca agctttaaaa aatatgggta aaaagctttc
     1801 tgaaaaagtt gtacgtagat tgatgactga ggaaaatcta tttgttaaat tttctagacg
     1861 aaaaaagtat tcttcgtatg ctggggaaat ttctcctgca caccccaatt tattaaatcg
     1921 gaattttaaa gcaaagaagc caaatgaaaa atggttaaca gatattactg aatttagaat
     1981 tctcgcaggg aaaatctact tatcaccact tgtcgattgt tacgatggtg ctattgttag
     2041 ttggactatt ggaacaaagc caacttgctt cactctatgt ctaaaaagag ttattcacca
     2101 gataattcag cgtgcgaagg tttctttgga cgattaaaaa acgagatgtt ttatcaacgt
     2161 gactggaaga atacaacaat caatcagttt ataaacaaat tggatgatta tattcattgg
     2221 tataataatc aacgaattaa gttatcttta ggaaggctaa gccctcttca atatcggaag
     2281 aagcaaagat atatttgtta aaaaggtcca aaaaaaatat ccgcaccccc ttttttttgt
     2341 aaaaaactat tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc
     2401 aagaa
//
LOCUS       DACEDO010000143         2397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEDO010000143 DACEDO010000000
VERSION     DACEDO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.150"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            206..2068
                     /locus_tag="IWO52_002813"
     CDS             206..2068
                     /locus_tag="IWO52_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0357028.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="IWO52_002814"
     CDS             2068..>2397
                     /locus_tag="IWO52_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0357029.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACEDO010000144         2350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEDO010000144 DACEDO010000000
VERSION     DACEDO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.151"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..93
                     /locus_tag="IWO52_002815"
     CDS             <1..93
                     /locus_tag="IWO52_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0357030.1"
                     /translation="TKAVMVNETARKAIDLHIENLRREIHRLEE"
     gene            90..395
                     /locus_tag="IWO52_002816"
     CDS             90..395
                     /locus_tag="IWO52_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357031.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTK
                     RRTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            395..709
                     /locus_tag="IWO52_002817"
     CDS             395..709
                     /locus_tag="IWO52_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357032.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            702..1232
                     /locus_tag="IWO52_002818"
     CDS             702..1232
                     /locus_tag="IWO52_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0357033.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1229..1498
                     /locus_tag="IWO52_002819"
     CDS             1229..1498
                     /locus_tag="IWO52_002819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357034.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            1628..1915
                     /locus_tag="IWO52_002820"
     CDS             1628..1915
                     /locus_tag="IWO52_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357035.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
BASE COUNT          909 a          338 c          514 g          589 t
ORIGIN      
        1 acaaaggcag tgatggtcaa tgaaacggcg aggaaggcaa tcgatttgca catagagaat
       61 ttaagaaggg aaatccatcg attggaggaa tgagcgtgga taagaaagca gcaatgaaac
      121 gaatcatcga actgacacat tctgagaatt ggcaagaaga caaagaaata gttgcagaag
      181 tccaaaagct cggtaaatca atgtggagtg aaaaaaccaa acggagaaca ccgagaaaaa
      241 ttgcaatctg gcatggtgac cgaattctag taacaggtac tgctgaacag ttatctgaaa
      301 ttactggatt aagcaaaaac attatctggg atagagctaa acgcgagaac gttgattcga
      361 aaggtcgtca atttaaacat tgggagaaga aataatggag gaactaatca caaaagtaga
      421 gcagtgggca aaagataagg gattggatca agctgattcc agcaagcaaa tgttaaaaac
      481 gatcgaagag attggggaag ttgccgcttc tctagctaga aaagatgaat atggtttaag
      541 agacggaatt ggagacgtag tagtaacctt gattatttta gctatgcaaa atgatatgga
      601 tttgtacgag tgtctgaacc aagcatacaa cgaaatcaaa ggacgcacag gaaaaatggt
      661 agatggtgta ttcgtgaagt cgagtgattt ggaggacagc gatgaataaa aaagtattaa
      721 ttgataaaca agcattgatt gatgaattaa tgaaaatacc tggtgtggga tctaatagtg
      781 acgctttaga aacgattaaa cgtttcccat cgtatgaacc gcagaagcca gttgtgccta
      841 agtttgtggc ggaactactc gactattatc gtcagtcaac agatgttgat ttattagctt
      901 tattgataac tttccatgat tggtattatc gcaaaactaa agacggtgag catgaagaag
      961 caattgattg gctagttgat catcctgaaa tttatatgcg tgcatggctg tttggctacg
     1021 agctcgagaa agagccgttg tattgcgtaa taatcaatgg tagatatctt gtcaaagtat
     1081 tcagcaatac aaacgttgtc atattagttc cagcagatga attttcacag tacgttactc
     1141 aaaaatttca attaactgaa aaacaaatca aagcaattga tgaaagatac tggccgtttg
     1201 ctgtgccagt ggatgaggtg gtagaaggat gagcgaccca tacattacta aattagaaaa
     1261 gttgtccgaa gattttgaga agaataaaaa taaactaact aaagataaac ttatagaact
     1321 ggttaggttg gttatcagag gagctattac aagagaaaag gcgcagcaaa acatagaaat
     1381 ttttgctcgt agtgtccgag atggattaga gaagaaagtt aaacaaaatg aacaacagac
     1441 accgcagaat aacaaaacta agaaaacggg aactgaatgt actaaagaca aagtttgaaa
     1501 aagaatatgg aatttcagca gaagaaacat ataaagtggc aagtcagtgt gttgctgatg
     1561 cgagcggcgc tattcgtaag tttggaattt cgatattaag tgatgatcgt aaatgggagg
     1621 aaaaagaatg aaactaaaag acggatttta ctccagcagt catggtatcg gcggtttaat
     1681 gctagatatg ccgacaaaga accctaaaac acgtgagaaa ccaaaattca aagtcggtga
     1741 catggttcgc tgtgaagcag aaggattcat ctatccattt tgtggatatg tagaacatct
     1801 ctataatcac tcagcaatca ttcgtattga aaacacgatg gaatgtgaca agtggttagc
     1861 gaaaagcaaa gagaatttag cagtagcgag attggtggat atggaggtta tataaacagc
     1921 gaaaaaatgt acttctaaga gaaaaattta ttttttataa aaataggaaa gtatcattat
     1981 gaaaaagacc acagagataa ataaacctac cacataaaaa atgaatattt cttgcgattc
     2041 aatataggta tatttaacaa tttgtacaat aaaaaatact ataggtaaaa aagataacat
     2101 gaggagctta tttgttctag agtgtttgac aaagtaaaaa aatgcgacga taccaataca
     2161 aacgggaatc caaaataaca gcatgctcca catagtcact caaccatctc ctatacgctt
     2221 tattatcaat agataatatc atttttcgta ataaagataa aaaaataagt aaggcttctc
     2281 attaaaatat aaaaaagaca gccgaccact gactgcctat ataagagtat tgaaataaaa
     2341 agctgctgat
//
LOCUS       DACEDO010000145         2350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEDO010000145 DACEDO010000000
VERSION     DACEDO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.152"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..543
                     /locus_tag="IWO52_002821"
     CDS             <1..543
                     /locus_tag="IWO52_002821"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0357036.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1016..1588)
                     /locus_tag="IWO52_002822"
     CDS             complement(1016..1588)
                     /locus_tag="IWO52_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0357037.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1604..2209)
                     /locus_tag="IWO52_002823"
     CDS             complement(1604..2209)
                     /locus_tag="IWO52_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ0357038.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          467 c          359 g          783 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaaatg tgtccaagat attgactcaa atccagtaat atcacaatag
      601 gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt tggttcttaa
      661 atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca atagagtcaa
      721 tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga cttacgctga
      781 acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg atggtatcct
      841 aatatagtaa gcgtggctag cataccttta attactttac cttttacagt taccatacac
      901 attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca tgctaataga
      961 ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt cttttttaac
     1021 tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg gttatagctt
     1081 caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg
     1141 tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt gcaaacattt
     1201 ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca ctcaacaacg
     1261 tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc aaaaccttga
     1321 tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc gtgttacgag
     1381 caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca gccaacagct
     1441 gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa atattcttct
     1501 tctctacgca gttctcaagc aactgttgaa tttgttcttt tgtactttga gaagcggttg
     1561 agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa tcatctatcg
     1621 tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata tataactcgc
     1681 gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc aaatcgttta
     1741 tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc caatctacaa
     1801 ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt cggccatttc
     1861 cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc tcttcaaaag
     1921 aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata actggtgcaa
     1981 atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa tcgtaaatat
     2041 ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact tcgatttcat
     2101 ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt tcttcaacct
     2161 tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg cgaaatctcc
     2221 ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc gattttgttt
     2281 atgtgtcaaa aactattaag tttctgaccc ccgatagatt tttcaagtgt ctctattttg
     2341 cacagtctag
//
LOCUS       DACEDO010000146         2340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEDO010000146 DACEDO010000000
VERSION     DACEDO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.153"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            134..346
                     /locus_tag="IWO52_002824"
     CDS             134..346
                     /locus_tag="IWO52_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357039.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            508..1026
                     /locus_tag="IWO52_002825"
     CDS             508..1026
                     /locus_tag="IWO52_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAQ0357040.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1033..1545
                     /locus_tag="IWO52_002826"
     CDS             1033..1545
                     /locus_tag="IWO52_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357041.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1816..>2340
                     /locus_tag="IWO52_002827"
     CDS             1816..>2340
                     /locus_tag="IWO52_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0357042.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          892 a          298 c          384 g          766 t
ORIGIN      
        1 taaaaacact atatattccg aaaagtgagt atctccattc ataaaataat caacatattt
       61 aaaaaatcta attagctttt ttagtcaatc taaagttctt tattctatga tataatgtta
      121 ctgtagaaag tttgtggcgg tttggcaaat ccaattgaag gagcgtggtg cctatgggct
      181 tattagctta tcggacgtac tctgtagaaa ggaaatgcct gatttgttct cattggatca
      241 aatcattgct attatcggag cagcagcagt agttattact gccgtagcga aagctagcga
      301 ttcgatttta gcttggttaa agttcaaaaa agaacaaaaa aaatagagcc gtctcaactt
      361 ttggcgaggt agacgagctc taaagcttta tttacatgcc atccgctttg aacggactac
      421 actgggcatg tgacagcatg ccctttcatt tatattatat ctcattcttt tatataatga
      481 aagtaaataa atcatggagg ataaaatatg gataaattta atagattaac attaataaat
      541 cagtttgaaa ttttaaaagc tttaaatcct aacgaagaaa aatactatgc tgaaaaaata
      601 gaaatactaa cagaaggtta cgaatatcac tatagtgaaa tatttgaaaa tatttctgat
      661 ccattgccag aagaagattc gcggtttgtt ctagacatat taaatatgta tagagatatt
      721 acttttagta aaaataaatt aaaagatata aattcaattg ataattatta tatacaattt
      781 aaaggttttg actttaatag ctctaccgaa tttaagttag cactttatgc acaatttttt
      841 attgaaaaat taaataggtt tcaagaaata gttgaagata gtgattttaa cacttttaat
      901 agccataaac cgatgagagg tacttatata aaatttctcg aaaattataa tgatttaaaa
      961 tctaccaata attatcaatt tggaaacttg tcttatgaga tgataagtaa agttctaagt
     1021 ttgtgatgat aaatgaaatt aaatagtata aatgatttat ttgctattat aggtgttatt
     1081 gctaacatac ttttttcgtg tttgaatttt agatttactc aaaagaacaa aaagaacaaa
     1141 ctcagtatag aacaaatgaa aaaagacatt gaaactgatt taaaaaatga ccataaagaa
     1201 agaagaaaaa ttgaagaata tacttcttta atggaaaagt gtaaagcaac tgatgcaatt
     1261 tttagctttt taagaaaaag tgatgagata atcacacgtg aaaatataga tttagaaaat
     1321 gagattagta aacttgaatc tatcttttcc gatataaatt ctcttaagta tttaatcgtc
     1381 gaagaaaatt cggatagctt tttagaattt gaaaaatatc taacagatct tatagataat
     1441 agaaataatg aattaatgag acaaaacatt gcaagattcc ttactaaatt agtttctgaa
     1501 tggaaaagta ttatttctca aaatatggat aaattaagat attaaggatg ttagttttaa
     1561 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
     1621 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
     1681 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
     1741 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
     1801 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
     1861 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
     1921 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
     1981 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
     2041 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
     2101 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
     2161 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
     2221 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
     2281 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
//
LOCUS       DACEDO010000147         2310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEDO010000147 DACEDO010000000
VERSION     DACEDO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.154"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..702
                     /locus_tag="IWO52_002828"
     CDS             25..702
                     /locus_tag="IWO52_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0357043.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
     gene            complement(839..1255)
                     /locus_tag="IWO52_002829"
     CDS             complement(839..1255)
                     /locus_tag="IWO52_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357044.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1258..1542)
                     /locus_tag="IWO52_002830"
     CDS             complement(1258..1542)
                     /locus_tag="IWO52_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357045.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(2242..>2310)
                     /locus_tag="IWO52_002831"
     CDS             complement(2242..>2310)
                     /locus_tag="IWO52_002831"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0357046.1"
                     /translation="CSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          747 a          355 c          416 g          792 t
ORIGIN      
        1 ttaacagtca aatatcaaaa tgaattgaat ttagtaccgc tgaaaaattt caatgctaag
       61 cagatggatt tattttttgc attatgcgct cgtatgaaag atcgtggttt gaaaaaagtt
      121 agattttctt tcgaggaatt aaaagagtta agtgattata agatgactgc tacaaaagcc
      181 tttgtcggtg atttagaaaa gctctataaa gatatgctta atcttactta ccgtactgaa
      241 gatgaggatt ctattcgcta ctttgttttg tttactggtt ttgaaatcaa taaaacaaaa
      301 aagtttgttg aagtcagtgt taatccggac ctagaatata ttattaatgg tttaacagct
      361 gaatttagta gatttgaatt atctgctttc acgtcgttgc gttctacgta tgcaaaaact
      421 ctattccgac tgcttatgca gtaccgttct actggttatt atgtagttcg tattgaagag
      481 tttaggagct taatggatat cccagaatac tatcaaatgg gtaacattga tcaaaaagta
      541 ttaaatcctg cattacgaga actaaaaaaa tacttcaatg atcttaaagt cacaaaaata
      601 aaagcaaaaa agggcaataa aatcgccaaa ttagaattta ctttttcaag cctaacgtca
      661 gatttgccaa agatttctct gcgtaactgg ttagaagaat aaataaaaaa gaactcgccc
      721 cccttacatt aagcaagtag acaagtccct tacaaccgac taagattgct agtacattct
      781 agcatattgt atggggcttc taaataaaaa acagatagga ataactccta tctgtttttt
      841 atcgttttag aaaatttgtt gggtcgaaat ttctagaagt gactgtttga ctggtttctt
      901 tttgatgatt ctttggtcga tttcgatttg atggatttag tttagcttcg attttttctt
      961 tcacttcttg atatacttta gaatttgtgc caataagaac gcctgtatct tttagatata
     1021 aatcagcaaa ttctgcgatg atttcagtca tgatggaaat ttcttttccc atagcattta
     1081 gttttgcctg attgctggta gtggtcgtgt tggtatcggg ttcataactc atacataaaa
     1141 agcgaatggc ttccgagtag ttattgatat ctggattttt ttcgatgatg gaatctaaaa
     1201 cgtcaacatg actagggtac aaagtaacat gtttttggac agaagttttt ctcatggtta
     1261 tatcattcct ccgataagca tttcaatgat ttgattattt gtgtagacat actcgcttaa
     1321 tatttgagta ttttctgttt ggatagcaat aaatttatcg agcaataaat cgaattggtc
     1381 ttttttgtaa gtcgtaagtt gacggtcaaa atcactttcg attaaaactt ttagatattt
     1441 atttcgactc catttttctc ctgttttatt cgtgagttct tggcaacgtt tgtctattgt
     1501 tgcaacaaac tttggatcaa tattccgaac taaaatgtcc ataaaaaatc tccttttgca
     1561 attatgtata atttatgtat accatacaaa tactatattt gtatagctgt tttttgccaa
     1621 aatgctatac ataagctacc cacgatggcg aaaaagtgat atcaaatttt tttctgagaa
     1681 acgtgatagg cacagcctat ctacccactt tttttagaaa gtgggtacac gtttccttat
     1741 gctctttaaa attcgggggg gagtgctgca cgtgtaatcg ataaccaaat ttaacagacg
     1801 aaaaattgtt ggaatataaa aatatgacac ccgaaagaaa acgatcgttg gaaaaattaa
     1861 tgcaactagt gaacgaagac ggcggaattt cagacgcagc ttttgacgca tggagcgaat
     1921 ttcatggaga atataattaa gtgggtttaa atggctctaa aatcgcttct aagagttttt
     1981 tagcttggac caatgaaata caccaaaaac taaataaaac gtcttaaatc gaaaaatacg
     2041 agctctcaga gcgactttat ttgtaaatgt tgcatataag aaataattac ggattttaga
     2101 cttttgtagg atatttttga aataaatatt tatttctgtt ataatttgat tgcaaataag
     2161 tgaacacgaa acacacgaaa tccccagaga ggtcggattc tctggggctt tttttgtggg
     2221 ctgagtcgcc ttaattaagt tttacttctt gtttcggtta ttcaaccagt aactgaacaa
     2281 agcgcttaaa agcccaacaa gaagggagca
//
LOCUS       DACEDO010000148         2064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEDO010000148 DACEDO010000000
VERSION     DACEDO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.155"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(51..1598)
                     /locus_tag="IWO52_002832"
     CDS             complement(51..1598)
                     /locus_tag="IWO52_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0357047.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IWO52_002833"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IWO52_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0357048.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          581 a          431 c          371 g          681 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagt
//
LOCUS       DACEDO010000149         2046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEDO010000149 DACEDO010000000
VERSION     DACEDO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.156"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..605
                     /locus_tag="IWO52_002834"
     CDS             <1..605
                     /locus_tag="IWO52_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0357049.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..>2046
                     /locus_tag="IWO52_002835"
     CDS             739..>2046
                     /locus_tag="IWO52_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0357050.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT          798 a          254 c          375 g          619 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 gattatatag aaatagaaga tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta
     1861 aaattaagaa ttagctttga aaaaaataca aagagaatag taggtgtaca actttgtagt
     1921 caagaagatt tatctggact attatcaatg ttttctcttg ctatagaaga acaaattaca
     1981 ttagacaaat tgaaaatgtt agattattta tttcatccta gttttagcca accctataat
     2041 tttatt
//
LOCUS       DACEDO010000150         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEDO010000150 DACEDO010000000
VERSION     DACEDO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.157"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IWO52_002836"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IWO52_002836"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0357051.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IWO52_002837"
     CDS             complement(1431..1823)
                     /locus_tag="IWO52_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0357052.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          452 c          342 g          662 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tact
//
LOCUS       DACEDO010000151         2001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEDO010000151 DACEDO010000000
VERSION     DACEDO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.158"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..376
                     /locus_tag="IWO52_002838"
     CDS             <1..376
                     /locus_tag="IWO52_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0357053.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="IWO52_002839"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="IWO52_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0357054.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2001
                     /locus_tag="IWO52_002840"
     CDS             1857..>2001
                     /locus_tag="IWO52_002840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0357055.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DACEDO010000152         1993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEDO010000152 DACEDO010000000
VERSION     DACEDO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.159"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            74..403
                     /locus_tag="IWO52_002841"
     CDS             74..403
                     /locus_tag="IWO52_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0357056.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            393..680
                     /locus_tag="IWO52_002842"
     CDS             393..680
                     /locus_tag="IWO52_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0357057.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            964..1821
                     /locus_tag="IWO52_002843"
     CDS             964..1821
                     /locus_tag="IWO52_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0357058.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          741 a          266 c          393 g          593 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
      421 atgtcattaa gtatttaaaa aagctaaaag agaagccatt gaaagaaaaa tttttgaatt
      481 taatttatga tgaaattgcg attgatccat ataagggtga acaaaaaact ggggatttat
      541 caggtatttg gtctagtggg ttcaaatatg cgggcacaac atatagagta gcttacgaga
      601 ttatagaaaa caaggttatt ccagtattgt tatgtggaac gcatgaaaac ttttatgaac
      661 agttgaaaaa attaatctag tgatttgttg tcgttcttaa gaaggacggc ttctttttat
      721 cgaaaatctg attcgtctac atgactaaag tcgcgagtat tctctgcttc tcgataaatt
      781 aaataacgtc attactttga ggaaagctat aaatcaatat atttcagctt ttccttattt
      841 atgtactacc ttttacttat taattataag gtagtacata tatatctttt tttggtagta
      901 actaaaaata aatatgttat aatagtatca aatgaatatt gctaaaagaa aagaggtagg
      961 aacgtggtag cgaagaaata tgtaatcggt aattttaaag gtggcgtagg taaaagtacg
     1021 tgcgcgcaaa tgttagggtt tgaatcagca gttaatgaag gaaaaagaac cttgataatt
     1081 gacttggata tgcaaggaaa tacttctgat gtgatgaact taacgcatat gaacttcact
     1141 aacgaagaag gaggcggaga aggtgaaccg attgtttacg aaaatactgt gactgacgtg
     1201 ttgataaaca atcttccacc taaagaggct gtctataaag taatcaataa cctctatata
     1261 cttccagcag acatgtcctt tgaaatgtat gatgattgga tcaaagacca atataatgat
     1321 tcattatccc aattcaaata tatgaaagaa aagctagaac cattatttga tgagtttgac
     1381 gtcatctatt tagatgtccc accttctatt tctgtatata gtaaatccgc gatgtacatt
     1441 gcggattggg caatcgtcgt cttacagacc caagtaaaat cgatgagaaa tgcgatgcaa
     1501 tatcttgagt atatggactt ttttgtgaaa gaatttgata caaatttata tgttgctggt
     1561 gttattcctt tcatgttgga aagcggtgac gcagtagatc aggaaatgta taaacaagcc
     1621 caagagattt atggggaaca tttgattaaa aatgttgtac tcaaaaatgc gcgcttaaaa
     1681 cgatacgacg gatcaggtat tacgatggag aagacaaaga aaggtctctt aaaacaatgg
     1741 gatcgcaaaa gccacgaatt atttatcagt attttgaaag aattggaaga acacgagaat
     1801 tggtatgcgg aggtggagta attggctgac atcaaaggac ttgatatttt tgggaacaac
     1861 aaaggactcg ccaaaataaa aaaagaagca gaagcaaaga aaaagcagca agcaattaca
     1921 ccatctgaaa ataagcaatt agaagtgcac caagttcaag aaataaaaac ttcctccaat
     1981 gttgaaagac aga
//
LOCUS       DACEDO010000153         1736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEDO010000153 DACEDO010000000
VERSION     DACEDO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.160"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(192..1484)
                     /locus_tag="IWO52_002844"
     CDS             complement(192..1484)
                     /locus_tag="IWO52_002844"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0357059.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          498 a          366 c          303 g          569 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga actttatctt tttagaatgg
     1621 ttattaaaaa agaggttgta acagaagtgg tcagcttcaa gaaataagaa ggaatgtacg
     1681 aaaattgctt ctcaaatttt tgtgaaattt cgacttattt ttcgaagaag ctactt
//
LOCUS       DACEDO010000154         1699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEDO010000154 DACEDO010000000
VERSION     DACEDO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.161"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            84..1292
                     /locus_tag="IWO52_002845"
     CDS             84..1292
                     /locus_tag="IWO52_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357060.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
BASE COUNT          645 a          210 c          277 g          567 t
ORIGIN      
        1 aattgaaaat taacgttaaa tcaaaaaaat atttacaaga tgtttattta agtagataat
       61 aaaagaaaaa agcaggtgaa attgtgacag cagaaattgg tattatgaat aaaagtggaa
      121 tcgtgttagc ttcagattca gcatctacga ttggagatag caaagtatat aatactgcaa
      181 aaaaattatt tactttggat tccatgcatt ctgtaggtat catgatatat ggtaatgctg
      241 aatttaatgg tattccttgg gaaataatca ttactcaata taagaaaagc attggtagtt
      301 ctgtttttaa tactttggaa gaatacgcgg ataattttat agaatttgtc aaaacagcat
      361 cttttattag aagtgaacag accgagcaag aacaaatgat tggggttttt caaaaaataa
      421 tttcaggttt atttgagagt atagaagtag atattaactt tttaataagc caaggtacac
      481 aaatagacaa agatgtccta gtgaagttat tacaaacaaa aatgaatacc aatttgtctc
      541 aacagtccca gacattcatt ttagatattg aaaaggcgtt atttcttagt aactatggag
      601 aaatattaaa agatattctt aatagtattt ctacaatgga aggtgtttca gaagctatat
      661 cagaggaaat tcaaagttac gtttatgaaa ttataattag agacgatgtt tattcttccc
      721 ctacaggaat agttatagca ggatatggaa gaatggatat tttccctaaa ctatattctt
      781 ataatatgtt tggatttgtt atgaatattc tgaaatactc agaatatgaa agtgctcaaa
      841 taggaaatga caatggttca ttaagatcta ctatattacc atttgctcag tctgatgtgg
      901 taaatactgt agttcaaggg gtagatccac aaattactaa ttatctgtcg agtcaagtcg
      961 atagttttga cgataacgga aaaaatacct atattaatat tataaagaat atttcagagt
     1021 ttcaacaaaa tcaattcata tttccgttac taaacatgat tgctttactc ccagttgaag
     1081 aaacagcaat tatcgcagag actttattaa atttaaccag ttttaaacga aaatatacta
     1141 cttctgtaga aacagtaggt ggcccaattg atgttttagc tattacacct aatgatggcc
     1201 ctatttggat taaaagaaaa cattattttg atattgataa taatattgga tacagattgc
     1261 gaaaggagag agcgaatgat tacaataatt aaacctcagg aaaaatcccc tgttttgaaa
     1321 tttaatgaga gcaatctagc ccataataaa atgagtataa ctgatacagt atttgatgta
     1381 atggttgagc aacaaaaaaa aattaataat aacaatagac aaagaaaaga aattaaccaa
     1441 tattaatgtt ttttctgttg cgttttttgc agtcataatt taaaacctcg ctcaatcttg
     1501 ggcgaggtct ttttttattt ttctgaaaag ttaaagtaaa taatagcata aaataatata
     1561 ttttacaaag aataagtaca atctagtttt ttgctattaa atgtgtaata ataaatatgc
     1621 catcacaaca tgaagaatga aatccattat tatctagtct agtccattct ttttgtttgc
     1681 agtatttgtg atggctttc
//
LOCUS       DACEDO010000155         1671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEDO010000155 DACEDO010000000
VERSION     DACEDO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.162"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWO52_002846"
     CDS             7..624
                     /locus_tag="IWO52_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357061.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          634 a          206 c          257 g          574 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg g
//
LOCUS       DACEDO010000156         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEDO010000156 DACEDO010000000
VERSION     DACEDO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.163"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWO52_002847"
     CDS             131..505
                     /locus_tag="IWO52_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0357062.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWO52_002848"
     CDS             complement(619..1248)
                     /locus_tag="IWO52_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0357063.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWO52_002849"
     tRNA            complement(1382..1454)
                     /locus_tag="IWO52_002849"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACEDO010000157         1637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEDO010000157 DACEDO010000000
VERSION     DACEDO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.164"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(183..1502)
                     /locus_tag="IWO52_002850"
     CDS             complement(183..1502)
                     /locus_tag="IWO52_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ0357064.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          526 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaatt
//
LOCUS       DACEDO010000158         1559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEDO010000158 DACEDO010000000
VERSION     DACEDO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.165"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..451
                     /locus_tag="IWO52_002851"
     CDS             <1..451
                     /locus_tag="IWO52_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAQ0357065.1"
                     /translation="NLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSG
                     LVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVF
                     KGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            696..>1559
                     /locus_tag="IWO52_002852"
     CDS             696..>1559
                     /locus_tag="IWO52_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0357066.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNL"
BASE COUNT          614 a          184 c          316 g          445 t
ORIGIN      
        1 aaacttagtg actgctgcga gagataacga taggttaaaa cagcatgtta gaaatctcat
       61 gagtactgat atggcgagag aatataaaaa attaagtaaa gaacatgggc aagttaaaga
      121 aaaatatagt ggtcttgtag agcgatttaa tgaaaatgta aatgattata atgagttgct
      181 tgaagaaaac aagtctttaa agtctaaaat aagcgattta aagcgtgatg tgagtttaat
      241 ctatgaaagc actaaggaat tccttaagga acgtacagac ggcttaaaag cctttaaaaa
      301 cgtttttaag gggtttgtag acaaggtaaa ggataaaaca gcacaattcc aagaaaaaca
      361 cgatttagaa cctaaaaaga acgaatttga actaactcat aaccgagagg taaaaaaaga
      421 acgaagtcga gatcagggaa tgagtttata aaataaaaaa agcacctgaa aaggtgtctt
      481 tttttgatgg ttttgaactt gttctttctt atcttgatac atatagaaat aacgtcattt
      541 ttattttagt tgctgaaagg tgcgttgaag tgttggtatg tatgtgtttt aaagtattga
      601 aaacccttaa aattggttgc acagaaaaac cccatctgtt aaagttataa gtgaccaaac
      661 aaataactaa atagatgggg gtttctttta atattatgtg tcctaatagt agcatttatt
      721 cagatgaaaa atcaagggtt ttagtggaca agacaaaaag tggaaaagtg agaccatgga
      781 gagaaaagaa aatcgctaat gttgattact ttgaacttct gcatattctt gaatttaaaa
      841 aggctgaaag agtaaaagat tgtgctgaaa tattagagta taaacaaaat cgtgaaacag
      901 gcgaaagaaa gttgtatcga gtgtggtttt gtaaatccag gctttgtcca atgtgcaact
      961 ggaggagagc aatgaaacat ggcattcagt cacaaaaggt tgttgctgaa gttattaaac
     1021 aaaagccaac agttcgttgg ttgtttctca cattaacagt taaaaatgtt tatgatggcg
     1081 aagaattaaa taagagtttg tcagatatgg ctcaaggatt tcgccgaatg atgcaatata
     1141 aaaaaattaa taaaaatctt gttggtttta tgcgtgcaac ggaagtgaca ataaataata
     1201 aagataattc ttataatcag cacatgcatg tattggtatg tgtggaacca acttatttta
     1261 agaatacaga aaactacgtg aatcaaaaac aatggattca attttggaaa aaggcaatga
     1321 aattagacta tgatccaaat gtaaaagttc aaatgattcg accgaaaaat aaatataaat
     1381 cggatataca atcggcaatt gacgaaactg caaaatatcc tgtaaaggat acggatttta
     1441 tgaccgatga tgaagaaaag aatttgaaac gtttgtctga tttggaggaa ggtttacacc
     1501 gtaaaaggtt aatctcctat ggtggtttgt taaaagaaat acataaaaaa ttaaacctt
//
LOCUS       DACEDO010000159         1489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEDO010000159 DACEDO010000000
VERSION     DACEDO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.168"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            225..1100
                     /locus_tag="IWO52_002853"
                     /note="aph(2'')-Ia"
     CDS             225..1100
                     /locus_tag="IWO52_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0357067.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1230..>1489)
                     /locus_tag="IWO52_002854"
     CDS             complement(1230..>1489)
                     /locus_tag="IWO52_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0357068.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          596 a          139 c          252 g          502 t
ORIGIN      
        1 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
       61 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      121 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
      181 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
      241 agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata
      301 gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga
      361 aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat ttagaaacta
      421 atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct atactaggtt
      481 ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg tcagaagaag
      541 aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac ggtttagatt
      601 atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa gagtatatat
      661 tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat atagaaagtt
      721 ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta tgccataatg
      781 attttagttg taatcatcta ttgttagatg gcaataatag attaactgga ataattgatt
      841 ttggagattc tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg
      901 aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata
      961 ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg
     1021 tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata
     1081 aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa gacattagta
     1141 ttaaatagtt taaaaaaatg aaaaataata aaggaagtga gtcaattcca gactcctgtg
     1201 taaaatgcta tacaatgttt ttaccatttc tacttatcaa aattgatgta ttttcttgaa
     1261 gaataaatcc attcatcatg taggtccata agaacggctc caattaagcg attggctgat
     1321 gtttgattgg ggaagatgcg aataatcttt tctcttctgc gtacttcttg attcagtcgt
     1381 tcaattagat tggtactctt tagtcgattg tgggaatttc cttgtacggt atattgaaag
     1441 gcgtcttcga atccatcatc caatgatgcg caagcttttg aatattttg
//
LOCUS       DACEDO010000160         1457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEDO010000160 DACEDO010000000
VERSION     DACEDO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.169"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..119)
                     /locus_tag="IWO52_002855"
     CDS             complement(<1..119)
                     /locus_tag="IWO52_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0357069.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
     gene            complement(130..327)
                     /locus_tag="IWO52_002856"
     CDS             complement(130..327)
                     /locus_tag="IWO52_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0357070.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(343..585)
                     /locus_tag="IWO52_002857"
     CDS             complement(343..585)
                     /locus_tag="IWO52_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0357071.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(620..757)
                     /locus_tag="IWO52_002858"
     CDS             complement(620..757)
                     /locus_tag="IWO52_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0357072.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            complement(759..1205)
                     /locus_tag="IWO52_002859"
     CDS             complement(759..1205)
                     /locus_tag="IWO52_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357073.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
BASE COUNT          385 a          319 c          230 g          523 t
ORIGIN      
        1 gagctacctt catttacacc taaattaaat tcattgttga tcgtataggc gaacccgtta
       61 attggcaata aaaaaagagc cattattagg ctcaatgata aaatgatttt cttcttcatt
      121 tttttcctcc tattttttta agttatatgc agacacacca gtgataacgc ctaaaaatgt
      181 tgctactgca ttgatagtga gtactgtcat atctgttcca ttccatccat acgcttttcc
      241 taacgtggct actaaaacag atgcagctgg taatactgtt aaaaccgtcc atttaatgac
      301 ttgataatac ttatcgggta aaatcatttc ttcttaactc ctttacaatt tagtcaagaa
      361 atagccaatg atcgtaatgc ctaaaccgat catgtaaccc cacgaccatt tattattggc
      421 tttcatttct ttgatatctt ctgcgttgtt tagcgctact gagtaggctt tgtctgccaa
      481 atcttttgca ctatcagctt tttctctaag tgattcgtag ttgtctaatt ttgtttcaat
      541 tcttactaat ctctccacaa cgtcttggag cgcttcttct ttcatgttcc accaactttc
      601 caacaaaaaa acgcatcact taagatgcgc tctcttcttt gctaataatt ctatctgctt
      661 cttcatctgt gatacatagt ggaacgaata gacgaacttg atcgtcagta aaacagcccc
      721 aatcatacat cattttcaca tcgctaaaac taaacatact actcacctcc ctttgaagct
      781 ggatttagtt gctctttaat ttctgaaatg tctttgctat tttgtaacga agcaagcatc
      841 atttttgaat tgatttgtgc taaactgtca gctttttctt tcaatgcagt attttcctgt
      901 ttaatcgcta catcgcttag catgagtttg gcatttagct gttttaggtt gccgttttca
      961 tgttcaagag cctcgtacat cgctttgaga ttgtttaaat cgttgtgatc taacgcgttc
     1021 gctaaaataa tccattggtt caatttagga tcaaacatct gatcagcaat cgttagcggt
     1081 tcgccatctg aacgaacccc ttcgagcggt ggctgatctg tgtaaggaac ggatacaagc
     1141 atgtcgtcca atacttttcc tgcatactct ccgccagtac ggccatattt ccaaatgttt
     1201 ttcatttatt tccctcccag taattgaatt tcggtttcca atttggaatc ggtggttcga
     1261 cttctgtgca ttcttccggt aaatgttctt catcattcac aatgatttgc tcgaatccgt
     1321 aaggttcaat tggtctatat gctgccttca tatcgattca cgcctttcta aatcgtgtaa
     1381 gtaattgcaa aggtataatc cgatccataa cttgagtttc ttcgccattt aatggctcca
     1441 tctgcaccaa tagataa
//
LOCUS       DACEDO010000161         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEDO010000161 DACEDO010000000
VERSION     DACEDO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.170"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            108..1040
                     /locus_tag="IWO52_002860"
     CDS             108..1040
                     /locus_tag="IWO52_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0357074.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IWO52_002861"
     CDS             1112..>1456
                     /locus_tag="IWO52_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357075.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACEDO010000162         1371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEDO010000162 DACEDO010000000
VERSION     DACEDO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.171"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..923)
                     /locus_tag="IWO52_002862"
     CDS             complement(<1..923)
                     /locus_tag="IWO52_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0357076.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYV"
     gene            complement(934..1140)
                     /locus_tag="IWO52_002863"
     CDS             complement(934..1140)
                     /locus_tag="IWO52_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0357077.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(1273..>1371)
                     /locus_tag="IWO52_002864"
     CDS             complement(1273..>1371)
                     /locus_tag="IWO52_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0357078.1"
                     /translation="KRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          389 a          286 c          222 g          474 t
ORIGIN      
        1 acatagactt tcttattagg gaagttacgc atgttaaaag atacatgata gccaacattt
       61 ccagaagtat tataagcttg atttacatct gatctataaa ttccatcagc tcttactcga
      121 gctaattctt tcccagtatt gtaatccata atgaagatat actcgtattt atagttagca
      181 atgtgccatc cagctacatg caaatttgca ttttcgattt ctccaaactg atcaatgtgg
      241 gcgtaattcg ttccatctgt cagtgtagga ttagctgcac cagctcgtgt tggatcaatg
      301 acaggcttgt tttcagaagt tgttggattt tcatcggtaa atccatgagc taaatcatat
      361 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      421 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      481 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      541 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      601 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      661 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      721 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
      781 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
      841 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      901 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      961 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
     1021 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
     1081 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
     1141 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
     1201 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
     1261 ctttcttaat tgtcaaatta agttagacag atcatcaata cttacgtaac tcaaaaatac
     1321 ttccaaaggt gttcgataat ccagtgattt tctaggaata ttatttcgtt t
//
LOCUS       DACEDO010000163         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEDO010000163 DACEDO010000000
VERSION     DACEDO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.173"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWO52_002865"
     CDS             141..398
                     /locus_tag="IWO52_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357079.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWO52_002866"
     CDS             complement(630..1043)
                     /locus_tag="IWO52_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ0357080.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          487 a          159 c          159 g          471 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatccc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaat
//
LOCUS       DACEDO010000164         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEDO010000164 DACEDO010000000
VERSION     DACEDO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.174"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWO52_002867"
     CDS             200..742
                     /locus_tag="IWO52_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357081.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IWO52_002868"
     CDS             832..>1246
                     /locus_tag="IWO52_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ0357082.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACEDO010000165         1244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEDO010000165 DACEDO010000000
VERSION     DACEDO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.175"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            52..>1244
                     /locus_tag="IWO52_002869"
     CDS             52..>1244
                     /locus_tag="IWO52_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0357083.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLN"
BASE COUNT          382 a          246 c          245 g          371 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaa
//
LOCUS       DACEDO010000166         1242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEDO010000166 DACEDO010000000
VERSION     DACEDO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.176"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>1242
                     /locus_tag="IWO52_002870"
     CDS             82..>1242
                     /locus_tag="IWO52_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0357084.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIY"
BASE COUNT          413 a          243 c          239 g          347 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct at
//
LOCUS       DACEDO010000167         1235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEDO010000167 DACEDO010000000
VERSION     DACEDO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.177"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IWO52_002871"
     CDS             414..947
                     /locus_tag="IWO52_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0357085.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          396 a          228 c          232 g          379 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcg
//
LOCUS       DACEDO010000168         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEDO010000168 DACEDO010000000
VERSION     DACEDO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.178"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1231
                     /locus_tag="IWO52_002872"
     rRNA            <1..>1231
                     /locus_tag="IWO52_002872"
                     /product="23S ribosomal RNA"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DACEDO010000169         1179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEDO010000169 DACEDO010000000
VERSION     DACEDO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.179"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            32..303
                     /locus_tag="IWO52_002873"
                     /pseudo
     CDS             32..303
                     /locus_tag="IWO52_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            471..>1179
                     /locus_tag="IWO52_002874"
     CDS             471..>1179
                     /locus_tag="IWO52_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0357086.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT          423 a          195 c          218 g          343 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttaaatcta tattttgata tacaaattac
       61 agcttcagat actaagttgc ataaaccttt tccggacccc atacaaaagg caattgatga
      121 attgggcata aaaaataaga aacgatttat attggtgatt cattatatga catgcaaagt
      181 gcgaaagctt gtgggatagc atttggttta gctaaatggg gagccaaaaa tatagaaaat
      241 tttcaaagtg tagatataat tattgattct cctcaagaaa tacttgattt gttagaaaaa
      301 tagacaaaga gcgtgagata aggatagggt ctcacgctct ttgtttttcg ccatttaaag
      361 attgaaattt ttgccatcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg
      421 tgctacactc aaaatagttc cgaccaaaga attataagga gtgagtacaa atgacctata
      481 cccatcttac aacggatgaa cttgtaataa tagagtctta tttcaaaatt aatcaatctg
      541 ttgctaaaac tgcccattgc ttgaatcgtt caagacaaac gatccataaa gtatatctgt
      601 tcttcaagca aggaaaatca gccttagaat attatcaaca gtataagaaa aacaaatcaa
      661 actgtggtag acgtccgctt gttttacccg aggaacaatc agaatatatt caaagaaagg
      721 ttgttcaagg atggacaccc gatgtgattg ttggtcgtgc agcgtttcct attagttgtt
      781 ctgctcgtac catttatcgt atgttcaaaa aggggctatt tgattcttct gacttaccga
      841 tgaaaggcaa gcgtaaaccg aatggacatc aagaaagacg tggaaaacaa actttccgcc
      901 gctctattca tgaacgtgaa aaggattata gccaattctc aaatgagttt ggtcaccttg
      961 aaggtgacac tatcgtaggt ctgaaacata aaagtgctgt aattacctta gttgaacgat
     1021 tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca agcgattgat attgaaaaaa
     1081 aattaaatca atggtttgaa tctgtaccga aaaacctatt caaatccatc acttttgatt
     1141 gtggaaagga attttcaaat tggaaacaga tcagtaatg
//
LOCUS       DACEDO010000170         1138 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEDO010000170 DACEDO010000000
VERSION     DACEDO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.180"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..231)
                     /locus_tag="IWO52_002875"
     CDS             complement(<1..231)
                     /locus_tag="IWO52_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0357087.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEK"
     gene            570..1079
                     /locus_tag="IWO52_002876"
     CDS             570..1079
                     /locus_tag="IWO52_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0357088.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
BASE COUNT          400 a          164 c          191 g          383 t
ORIGIN      
        1 tttttcctca gcaggaaaaa tgatttcggt tctcccacaa cgtcgcttat tttctttgta
       61 ttgttcaaag tattcaagtg ctgttccacc acatttgaga aagttataga cattgtaaat
      121 tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt cttgatgaaa
      181 atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca tttatgttca
      241 ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg gtttttttat
      301 ttgtctcgct taattataca aacggcgtga taaaaaacga gaaatgaatt ataggtttag
      361 acctttatca tttctcgttt tattttattg tatcaaggct ctatcataag tggtaaaatc
      421 tacttttaaa tcacatgtaa tcattgataa gtccagtgtt ttagcggata atcagctttt
      481 tatttatttt ttcttataaa tttgataaat agaaattagt atagccctaa acaaattatt
      541 tgtgttttat tgtttagggc tatactatat tggtacaagg aatagaaaga ggtgtttatg
      601 tggttgataa aattcagttt aatcaagtta tagaagaatt catcaaagta aataatttaa
      661 tgacccatat tcaaaaacga ccaatcagaa taactaatga tctaaaactc agtacgagta
      721 tgattcattt gattgatgtg attggaaatt atcctgaaac tagtattacg gaattagctg
      781 atcgattagg cgttaccaaa ggagctgtat cccaacagat tcccacttta gagaaaatga
      841 atcttataag catttctcaa aaaaaaggaa ataagaaaac taaacttgta tcgttaacaa
      901 aatatggtcg aaaggtgtac gaatcacata attatttaca tgaagagctt tatagctcaa
      961 taaatgaagc gttagaatca ttctcaccag accaaatttc aaccattaaa cagattctag
     1021 aaaaagtatc ttatacaatc aatgaatatc aagtaagtct gacaagaaag gaagattgac
     1081 atgaaaaaaa tgatttttga atcggctaca ctagactaga cagaaaaaat aaggtgtg
//
LOCUS       DACEDO010000171         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEDO010000171 DACEDO010000000
VERSION     DACEDO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.181"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWO52_002877"
     CDS             complement(36..992)
                     /locus_tag="IWO52_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357089.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACEDO010000172         1020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEDO010000172 DACEDO010000000
VERSION     DACEDO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.182"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..>1020
                     /locus_tag="IWO52_002878"
     rRNA            2..>1020
                     /locus_tag="IWO52_002878"
                     /product="23S ribosomal RNA"
BASE COUNT          316 a          204 c          293 g          207 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
//
LOCUS       DACEDO010000173         1001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEDO010000173 DACEDO010000000
VERSION     DACEDO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.183"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            369..1001
                     /locus_tag="IWO52_002879"
                     /pseudo
     CDS             369..1001
                     /locus_tag="IWO52_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac c
//
LOCUS       DACEDO010000174          951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEDO010000174 DACEDO010000000
VERSION     DACEDO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.184"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            579..>951
                     /locus_tag="IWO52_002880"
     CDS             579..>951
                     /locus_tag="IWO52_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0357090.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSM"
BASE COUNT          303 a          138 c          220 g          290 t
ORIGIN      
        1 tttgtctggt agaaaataat tagcagattt acaagtggga aataaaaagc tgaaagaaga
       61 aaattgcatt tcaaactaca ttttctacaa tagctctaaa atggcatatt ttgcgttctg
      121 agcggtttta gcgtttattt cgtttagtta tcggcacaat cggtgaaaca cgttagagga
      181 gcgaaaatgg agcctgtaag gcgactggta gcggaacggt cgtgttagat tgtgtagccg
      241 atagctgaac gaaataaatg ctggcagcgc cattctattt cggcaggagg aggctcaagg
      301 ggttcgggga atgaaattcc ctgaggtctt tttatttttt ctagaggagt gctataatca
      361 aagaaaatga gaaaaaagag taaaaaaata gtcgagcagt gttgacgcac tctcgacctt
      421 tacggaaact acttcaccta gttccgtaac tcaagttcat tatacgaata tttttaggtt
      481 tcttcaagct agagattttt ggatgtggat ttttagagtt acgtgaactc tggaaattct
      541 ttccaagaat ctcttttttc gttgaggagg agctattttt gagtaaaaaa ggcataatcg
      601 taaatgagtg ggaaaaactt aaccgtattt ctaacaggcg gttaagtgtt gacgataagg
      661 tacaccccct aaaattaagt tggtcaatcg atcgaattac aatcgtcgga aaactgaatg
      721 ataccatagc ttattggacg caaaatggag ttgtgtttgt cgattttgag caattaatgc
      781 gtttgaatga aaatcaaggg tatttgaagt cggttggtac aaatggttgg caattatttg
      841 accaatatgg agaagatatc gcctatatcg aaatgttaaa atttcaccct gacaaaggtc
      901 gtattgattt taatccaaat aaaattggcg agtttttaaa ggggtcgatg a
//
LOCUS       DACEDO010000175          910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEDO010000175 DACEDO010000000
VERSION     DACEDO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.185"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..355
                     /locus_tag="IWO52_002881"
     CDS             <1..355
                     /locus_tag="IWO52_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357091.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="IWO52_002882"
                     /pseudo
     CDS             615..910
                     /locus_tag="IWO52_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DACEDO010000176          876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEDO010000176 DACEDO010000000
VERSION     DACEDO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.186"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(555..869)
                     /locus_tag="IWO52_002883"
     CDS             complement(555..869)
                     /locus_tag="IWO52_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357092.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          333 a          127 c          129 g          287 t
ORIGIN      
        1 tggttgttgc cacataaagc cataaacaat caaccagtag aatgcgtggc aacaaacctg
       61 ttatcgcata tcttggagtg tgactattta tgagtgatag tgaagatatg cgtctccata
      121 tatgtatggt tgagacagat aaacatgtat gaaaaacgtt gttaaatcaa cgttttttta
      181 ttttggtcaa ggagagaaaa gtagagaaat acaaaatttt ttgcattgat tttgcattca
      241 aaaaaacatt caaacattta gaaatcccca atacgtattc aggaaaatct attttaaggt
      301 ttacaaaaag aacttttgtt cgtacactct ttttgaggtg aacttttatg ctgatggaag
      361 gaaaaacaca attatggttt aagcatgttc cttcaaataa gttttttaat tataaactct
      421 agagaaatta attcatccta acataaaata attgttcact caaaagtttt tctagaacaa
      481 ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt cctgtactaa
      541 aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag tagttgtgta
      601 ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga aacgtctata
      661 acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc tccgtaaaaa
      721 atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt tctaaaacct
      781 atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa tattgctatt
      841 attaagataa aattactgta aaaatccaaa agctat
//
LOCUS       DACEDO010000177          854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEDO010000177 DACEDO010000000
VERSION     DACEDO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.187"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>854
                     /locus_tag="IWO52_002884"
     CDS             82..>854
                     /locus_tag="IWO52_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ0357093.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQA"
BASE COUNT          282 a          157 c          163 g          252 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctt
//
LOCUS       DACEDO010000178          807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEDO010000178 DACEDO010000000
VERSION     DACEDO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.188"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWO52_002885"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWO52_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0357094.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          297 a          126 c          152 g          232 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcagg
//
LOCUS       DACEDO010000179          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEDO010000179 DACEDO010000000
VERSION     DACEDO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.189"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IWO52_002886"
     CDS             <1..782
                     /locus_tag="IWO52_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0357095.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          244 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc ttt
//
LOCUS       DACEDO010000180          774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEDO010000180 DACEDO010000000
VERSION     DACEDO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.190"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            29..>774
                     /locus_tag="IWO52_002887"
     CDS             29..>774
                     /locus_tag="IWO52_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0357096.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT          280 a          145 c          133 g          216 t
ORIGIN      
        1 accaattcac agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac
       61 tctagtcact aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa
      121 cacactccta cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg
      181 ttttaattca ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg
      241 tgaattaaca ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc
      301 agcctacaaa agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg
      361 tgttacgatg tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc
      421 acaaaccatg tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag
      481 agccttaaat gataagtatg tcgctatttt tatggacgct acttacattc cactaaaacg
      541 tcaaaccgta tccaaagaag cgatttatat tgccattggt atacgagaag acggcactaa
      601 agaagtactg agttatgcga ttgctccaac tgaatcaaca tacgtttgga atgagctgct
      661 acaggatatt aactccagag gagttcaaga agtcttgctt tttattacgg acggcttaaa
      721 aggcatgaaa gatactatcc atcaaattta tcctaaagca aaatatcagc attg
//
LOCUS       DACEDO010000181          736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEDO010000181 DACEDO010000000
VERSION     DACEDO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.191"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..734
                     /locus_tag="IWO52_002888"
     CDS             <1..734
                     /locus_tag="IWO52_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0357097.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          318 a           94 c          122 g          202 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagtt
//
LOCUS       DACEDO010000182          725 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEDO010000182 DACEDO010000000
VERSION     DACEDO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.192"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..725
                     /locus_tag="IWO52_002889"
                     /pseudo
     CDS             1..725
                     /locus_tag="IWO52_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          250 a          146 c          156 g          173 t
ORIGIN      
        1 gaacgcatcg aggagttgga gaaacgagtc cgttcccttc aaattgaaaa tgccttttta
       61 aaagaattga ggaagctgcg aaaacaggaa gcccaacaac gacgaatgaa gcaatcgcac
      121 gaatcatcgc aagcctccga ggaccgttta aattagttga gcttctagaa actttaaagt
      181 tccctaaggc aatctttatg tactggcaaa aacgattgga ccgtaaaaat ccagatcaag
      241 agattgaagc ggaaatgctt aaagttcggg agaagcacaa ggattatggc tgtttacgga
      301 tgaccaatga actaagaaac cgcggtttct ctatcaataa aaagaaagtt cagcgcctga
      361 tcaagaagct aggaataaaa gtaacgacat acacacgtaa atcacggcgc tacaattctt
      421 atcgaggaca agtcggagca gtagccaaaa atcggatcca tcgtcgtttc tacaccagta
      481 tctgccatca gaaaatcacg acggatacaa ctgaatttaa ataccatgaa gtggatgcca
      541 aaggcattat ccgtcaaaag aaattgtatc tagatccttt tatggatatg ttcaatagtg
      601 agattttatc ctatcgtatc tctgaaaagc ccaatgcatt agcggtaatg gaaggtttgg
      661 aggaagctat ccagaccact aacgattgtc cctatcgtcg tacgtttcac tcagatcaag
      721 gttgg
//
LOCUS       DACEDO010000183          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEDO010000183 DACEDO010000000
VERSION     DACEDO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.193"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            271..669
                     /gene="spxA"
                     /locus_tag="IWO52_002890"
     CDS             271..669
                     /gene="spxA"
                     /locus_tag="IWO52_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0357098.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          250 a           99 c          130 g          216 t
ORIGIN      
        1 gagataaaat aatagtacag ttttatacgc tgtttcatat gtttagaaaa tttgaattag
       61 atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt tactgtatat
      121 taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag ctatttaaaa
      181 gttgattatg tataatagaa acagatggga gattattatg atcttctgtt acaaaaaagt
      241 gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc cccaagttgt
      301 acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt tgttgaacgt
      361 aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca aatgacagaa
      421 gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact gaacatggat
      481 ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc tggattgctt
      541 cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga agatgaaatt
      601 cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca attgatggct
      661 ggattataga aggataatac gaaacttgga aaaga
//
LOCUS       DACEDO010000184          680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEDO010000184 DACEDO010000000
VERSION     DACEDO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.194"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..596
                     /locus_tag="IWO52_002891"
     CDS             <1..596
                     /locus_tag="IWO52_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0357099.1"
                     /translation="KQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPVSFKPI
                     YGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRGKATD
                     WHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEE
                     SQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
BASE COUNT          225 a          104 c          166 g          185 t
ORIGIN      
        1 tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca gatattgctt
       61 gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt gaccctgtgt
      121 cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg ggtagtcgtg
      181 ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacga aaaaaacaga
      241 ttgtaccaaa agaaatcgaa acatggtggc gtatagaaat gcagctaaga cgtggcaaag
      301 caacagattg gcatgcgatg gttcgggaaa gtttagatag ctttgcaagt cctcattact
      361 tgcctggaga tgtaaaacct gttgatagaa ttatgataaa agggctgaat caagatcatt
      421 cagagtgggc gtatattagc agaaatttga aatatagatt gcgcaaattg ttgaaagaag
      481 aaagccaaaa cgatgagttg acgaatcatt tacgagagac gtttaaggaa tctgcaaacg
      541 acttgaaaat agaattagac acgtggcttt tgggtttgga cgtgacagag aagtgatacc
      601 gaataagaaa agtcagatga gtgagagtaa atttacgtca atgtttaatg acgtgagttt
      661 acgataagcg attattttgc
//
LOCUS       DACEDO010000185          670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEDO010000185 DACEDO010000000
VERSION     DACEDO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.195"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            154..417
                     /locus_tag="IWO52_002892"
     CDS             154..417
                     /locus_tag="IWO52_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0357100.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="IWO52_002893"
     CDS             417..>670
                     /locus_tag="IWO52_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0357101.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DACEDO010000186          634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEDO010000186 DACEDO010000000
VERSION     DACEDO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.196"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(82..171)
                     /locus_tag="IWO52_002894"
     tRNA            complement(82..171)
                     /locus_tag="IWO52_002894"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Ser,seq:tga)
     gene            complement(194..265)
                     /locus_tag="IWO52_002895"
     tRNA            complement(194..265)
                     /locus_tag="IWO52_002895"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(230..232),aa:Glu,seq:ttc)
     gene            complement(287..374)
                     /locus_tag="IWO52_002896"
     tRNA            complement(287..374)
                     /locus_tag="IWO52_002896"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Ser,seq:gct)
     gene            complement(399..472)
                     /locus_tag="IWO52_002897"
     tRNA            complement(399..472)
                     /locus_tag="IWO52_002897"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(436..438),aa:Ile,seq:gat)
     gene            complement(486..556)
                     /locus_tag="IWO52_002898"
     tRNA            complement(486..556)
                     /locus_tag="IWO52_002898"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Gly,seq:tcc)
     gene            complement(558..633)
                     /locus_tag="IWO52_002899"
     tRNA            complement(558..633)
                     /locus_tag="IWO52_002899"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(598..600),aa:Asp,seq:gtc)
BASE COUNT          174 a          176 c          152 g          132 t
ORIGIN      
        1 ggagcaggat ttgaacctac gaccttcggg ttatgagccc gacgagctac cagactgctc
       61 catcccgcga taatatgaaa aaggaggata agggattcga acccttgcac ggttttaccc
      121 gcctgacggt tttcaagacc gttcccttca gccggacttg ggtaatcctc catgtaaaac
      181 tatattaaat taatgacccg tacgggattc gaacccgtgt taccgccgtg aaagggcggt
      241 gtcttaaccg cttgaccaac gggccaggta aaacaaaata ttgaaacgga gaaggaggga
      301 tttgaaccct cgcgccggtt tcccgaccta cacccttagc aggggcgcct cttcagccac
      361 ttgagtactt ccccaaaacc aataagattt tgtactaatg ggcctaaatg gactcgaacc
      421 atcgacctca cgcttatcag gcgtgcgctc taaccagctg agctataggc ccataaaaaa
      481 ctaaaagcgg gtgacgagaa tcgaactcgc gacaacagct tggaaggctg tggttttacc
      541 actaaactac acccgcatgg cggttccgac gggaatcgaa cccgcgatct cctgcgtgac
      601 aggcaggcat gttaacccct acaccacgga acca
//
LOCUS       DACEDO010000187          630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEDO010000187 DACEDO010000000
VERSION     DACEDO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.197"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>630
                     /locus_tag="IWO52_002900"
     rRNA            <1..>630
                     /locus_tag="IWO52_002900"
                     /product="16S ribosomal RNA"
BASE COUNT          156 a          144 c          196 g          134 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct
//
LOCUS       DACEDO010000188          618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEDO010000188 DACEDO010000000
VERSION     DACEDO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.198"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>618
                     /locus_tag="IWO52_002901"
     CDS             <1..>618
                     /locus_tag="IWO52_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357102.1"
                     /translation="SGKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVI
                     TNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTV
                     EPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQM"
BASE COUNT          223 a          100 c          123 g          172 t
ORIGIN      
        1 tccggtaaat cattagaagc attagaactc gaacaagagg aaatcagaag atttcagaat
       61 tgctccttgt accctgttta tcatggaagc gcaaaaagca acatagggat tgagcagctt
      121 atagaagtga taacgaataa attttattca tcaacataca gaaagaagtc tgaactttgc
      181 ggaaatgtct tcaaaattga atattcggaa gaaagacaac gtcttgcata tgtacgcctt
      241 tatggcggaa tcctgcattt gcgggattcg gttagaatat cggaaaagga aaaaataaaa
      301 attacagaaa tgtatacttc aataaatggt gaattatgta aaattgataa ggcttattcc
      361 ggggaaattg ttattttgca aaatgagttt ttgaagctaa atagtgttct tggagataca
      421 aagctattgc cacagagaga gagaattgaa aatccgctcc ctctgctgca aacaactgtt
      481 gaaccgagca aacctcaaca aagggaaatg ttacttgatg cacttttaga aatctccgac
      541 agtgacccgc ttctacaata ttatgtggat tctacgacac atgaaattat actttctttc
      601 ttagggaaag tacaaatg
//
LOCUS       DACEDO010000189          614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEDO010000189 DACEDO010000000
VERSION     DACEDO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.199"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            194..424
                     /locus_tag="IWO52_002902"
     CDS             194..424
                     /locus_tag="IWO52_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357103.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          235 a           94 c           91 g          194 t
ORIGIN      
        1 acaaaaccac tatattctat tatgtatcta ataccctcaa aacagctata aatcaacaat
       61 accgacatat aataaacact atgttcaatc tgagatatat caaaggtaaa cgtatttttg
      121 cccccttttt gcccctcaaa tatcgcttta caacgagaac gtaagttcgt ataatcgttt
      181 tgaggtgatt tttatgatgg aagactttat tagaaaaaat atcagtgatg aatatgcaga
      241 cttttatgag caaagcagca aaaaagataa attccagatg gatgtttcaa tcttagctat
      301 attagctttt tccgaaaata accaacctgt aattgcgaaa aaagaaacag tattatctga
      361 aggcaaaata aaaactcgat atatattaga ggtagaaact aagtttaaaa atagatcgga
      421 gtaatggcta tgctttttaa tgagacacaa ttatggttta aatttgatcc ttcgaataga
      481 tttatcaaag atttttataa ggtgtgggat tcagaagttt tctttctagc aatcgaagat
      541 agcttattaa tcaatctcta ctattctaat aagaactact ttaaaatccc tgctgcaaaa
      601 actagaatga agaa
//
LOCUS       DACEDO010000190          591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEDO010000190 DACEDO010000000
VERSION     DACEDO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.200"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(316..579)
                     /locus_tag="IWO52_002903"
     CDS             complement(316..579)
                     /locus_tag="IWO52_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ0357104.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          103 c           78 g          186 t
ORIGIN      
        1 cttccttttt tgtatggatt ttttcattta cacaaaatat tttatactct cctgaatttt
       61 ttcatgttga aaacatcact aatcaaaggc aaattataat ttagaaatag aatgaaaaca
      121 ctaaatgaaa atagaacatt agaaaaaata gaatcaaaaa aacccaagac tagtgccgaa
      181 ataagggaaa caatcgaagc tacaaaaaat aatgatttcg ttgaacagga ccaatttatt
      241 tgtttccgat atttctcaaa aagaactttc tttgggaaag ttaaaggcga ctgtaaagtc
      301 gcctttagtc cttttttacc aagctgctgc atatttagct ccatattttt taattaatga
      361 tttggctgta gcaacaatac cagctgtaat taatccgcct ccaacaactg cagcaacaat
      421 agaaatgatt gtagcaaccg tgctaccagt attaattatg tcaataactt ttttagctgc
      481 ataagaagaa attccaatgt tagcacttag cattggtaca ccaacaaaga atcccgcaaa
      541 acctaaaatt ggtaaaacta ataataaatt ctttttcata ataattcctc t
//
LOCUS       DACEDO010000191          573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEDO010000191 DACEDO010000000
VERSION     DACEDO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.201"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..573
                     /locus_tag="IWO52_002904"
                     /pseudo
     CDS             1..573
                     /locus_tag="IWO52_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          227 a           98 c          108 g          140 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggc
//
LOCUS       DACEDO010000192          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEDO010000192 DACEDO010000000
VERSION     DACEDO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.202"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..>536
                     /locus_tag="IWO52_002905"
     CDS             51..>536
                     /locus_tag="IWO52_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0357105.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YL"
BASE COUNT          194 a           84 c          108 g          150 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctg
//
LOCUS       DACEDO010000193          529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEDO010000193 DACEDO010000000
VERSION     DACEDO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.203"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWO52_002906"
     tRNA            complement(5..90)
                     /locus_tag="IWO52_002906"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWO52_002907"
     tRNA            complement(107..178)
                     /locus_tag="IWO52_002907"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWO52_002908"
     tRNA            complement(190..262)
                     /locus_tag="IWO52_002908"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWO52_002909"
     tRNA            complement(280..361)
                     /locus_tag="IWO52_002909"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWO52_002910"
     tRNA            complement(369..441)
                     /locus_tag="IWO52_002910"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWO52_002911"
     tRNA            complement(445..517)
                     /locus_tag="IWO52_002911"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          136 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactta
//
LOCUS       DACEDO010000194          511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEDO010000194 DACEDO010000000
VERSION     DACEDO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.204"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..404
                     /locus_tag="IWO52_002912"
     CDS             <1..404
                     /locus_tag="IWO52_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0357106.1"
                     /translation="AVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDIN
                     RSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTL
                     GYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          156 a           99 c          109 g          147 t
ORIGIN      
        1 atgccgttct agtaaaaaaa gcatttagcc gtatcaaaca acctctgaca gaggttgaga
       61 tttttcatac tgatcgtgga aaagagtttg ataaccaagc tattgatgaa ttattaacaa
      121 cttttgacat caatcgatca ttgagtcata aaggctgtcc ttttgataat gccgtagctg
      181 aatcaactta taagtcgttg aaagtagaat ttgtctatca atacacattt gaaaccttac
      241 aacaattgga tttggagtta tttgactatg tcaattggtg gaaccacctt cggttgcacg
      301 gtacacttgg ctacgagaca ccggttggtt accgtaacca gagattggcg cagcgaatcc
      361 ttgataatga gctcggatgt gctaacgcta gcgaggcagt ctaactttaa ctgttagctc
      421 ctgccgaaga tcgtcacatc cgaggaggct cattgtcaag gacaatcgga atagcatacg
      481 gaaagaagtt ctgcacctta taaaatttgt c
//
LOCUS       DACEDO010000195          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEDO010000195 DACEDO010000000
VERSION     DACEDO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.205"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..413)
                     /locus_tag="IWO52_002913"
     CDS             complement(<1..413)
                     /locus_tag="IWO52_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357107.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPF"
BASE COUNT          184 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaacggct ttccaaatga tgaaatacct aaatctaact tattaagaaa gttgaaattt
       61 ttatattcag gaaaccattt attaatttca tgtgcagcac gtaatgcatt ccctggagtt
      121 gtcaaagaga aaattaattc tgctaagctt attattgaaa atactgataa cttataatta
      181 tatcgtccat tgacaatcaa atataaacta actatacttg ttagaacaaa aaaacaggcg
      241 ttgacttgtt cttgattagt taaaaatatc aataaaggaa tacttaccca atatattttt
      301 ctatttattt cttctcctct taagagttga aaaaacggat aaaaagttag taaactaaat
      361 gctactggcc actgatagtt caaggtggtt gctctccaac cggtttcccc cattactgtc
      421 aatggtatta aacagtatat agagcaaacc aataataaat ttttaacatt tattttctca
      481 aaaacat
//
LOCUS       DACEDO010000196          473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEDO010000196 DACEDO010000000
VERSION     DACEDO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.206"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>473
                     /locus_tag="IWO52_002914"
     CDS             <1..>473
                     /locus_tag="IWO52_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0357108.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          161 a           92 c           95 g          125 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DACEDO010000197          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEDO010000197 DACEDO010000000
VERSION     DACEDO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.207"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>468
                     /locus_tag="IWO52_002915"
     CDS             <1..>468
                     /locus_tag="IWO52_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0357109.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DACEDO010000198          461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEDO010000198 DACEDO010000000
VERSION     DACEDO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.208"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            237..>461
                     /locus_tag="IWO52_002916"
     CDS             237..>461
                     /locus_tag="IWO52_002916"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734812.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0357110.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLF"
BASE COUNT          157 a           86 c           59 g          159 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt t
//
LOCUS       DACEDO010000199          425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEDO010000199 DACEDO010000000
VERSION     DACEDO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.209"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(87..>425)
                     /locus_tag="IWO52_002917"
     CDS             complement(87..>425)
                     /locus_tag="IWO52_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0357111.1"
                     /translation="LERMAGRSLIDIKSPIITDMPKAPKHGNKAEDAIIQMMDIEAER
                     DAILAALMALSLISRQILYYSFCVPDSFSNYRISREVGYSERSIQRMKSEALIEFAEA
                     YKHGRIIAYK"
BASE COUNT          118 a          113 c           67 g          127 t
ORIGIN      
        1 agacctatca ctaatctttc gacactacca taatatcact ggtaaatagc taaaaaccgc
       61 catcattccg ccaaaaagcc gccaaattat ttataagcaa ttattcttcc gtgtttatat
      121 gcttctgcaa actctattag agcttccgac ttcatccgtt gtatacttct ttctgaataa
      181 cccacttcac ggctaattct gtagtttgag aagctatctg gcacacaaaa gctatagtag
      241 agtatctgac gactaatcag actaagagcc atcaaggctg ctagaatcgc gtctctctcc
      301 gcttctatat ccatcatctg aatgattgcg tcttctgcct tattgccgtg cttcggcgcc
      361 ttcggcatat ccgtaataat cggcgactta atatctatca aagagcgacc tgccatccgc
      421 tccaa
//
LOCUS       DACEDO010000200          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEDO010000200 DACEDO010000000
VERSION     DACEDO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.210"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWO52_002918"
     CDS             complement(<1..339)
                     /locus_tag="IWO52_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0357112.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACEDO010000201          399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEDO010000201 DACEDO010000000
VERSION     DACEDO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.211"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>399
                     /locus_tag="IWO52_002919"
     CDS             <1..>399
                     /locus_tag="IWO52_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0357113.1"
                     /translation="QETPVAIVAKQLKRGRQTIYNVYNFLKCGGTALEYFEQYKENKR
                     RCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFSCSVSTLYRRFKTGEFNVLH
                     LPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          147 a           73 c           83 g           96 t
ORIGIN      
        1 tcaagaaact ccggttgcta tcgttgcgaa gcagcttaaa cgtggacgcc aaacaattta
       61 caatgtctat aactttctca aatgtggtgg aacagcactt gaatactttg aacaatacaa
      121 agaaaataag cgacgttgtg ggagaaccga aatcattttt cctgctgagg aaaaagaata
      181 cattgaaaaa agatcaactg aaggttggac cccagacgtc attattggcc gtgcagagcg
      241 aaccttctct tgttcagtaa gtaccctcta tcgccggttt aagacgggag aattcaatgt
      301 tttacattta ccgatgcaag gaaaacgaaa accaaatggt tataaggaaa aacgtggaaa
      361 acaggcattc aaaagaaata tttctgaacg taaaaaaga
//
LOCUS       DACEDO010000202          394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEDO010000202 DACEDO010000000
VERSION     DACEDO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.212"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..285
                     /locus_tag="IWO52_002920"
     CDS             <1..285
                     /locus_tag="IWO52_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0357114.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DACEDO010000203          367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEDO010000203 DACEDO010000000
VERSION     DACEDO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.214"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          113 a           74 c           61 g          119 t
ORIGIN      
        1 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
       61 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
      121 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
      181 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      241 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
      301 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
      361 cagggca
//
LOCUS       DACEDO010000204          362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEDO010000204 DACEDO010000000
VERSION     DACEDO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.denovo.215"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>362)
                     /locus_tag="IWO52_002921"
     rRNA            complement(<1..>362)
                     /locus_tag="IWO52_002921"
                     /product="16S ribosomal RNA"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DACEDO010000205         9012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.guided.092, whole genome shotgun sequence.
ACCESSION   DACEDO010000205 DACEDO010000000
VERSION     DACEDO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.guided.092"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(106..777)
                     /locus_tag="IWO52_002922"
     CDS             complement(106..777)
                     /locus_tag="IWO52_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357115.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(770..1264)
                     /locus_tag="IWO52_002923"
     CDS             complement(770..1264)
                     /locus_tag="IWO52_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0357116.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1284..2504)
                     /locus_tag="IWO52_002924"
     CDS             complement(1284..2504)
                     /locus_tag="IWO52_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0357117.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2651..4486
                     /gene="lepA"
                     /locus_tag="IWO52_002925"
     CDS             2651..4486
                     /gene="lepA"
                     /locus_tag="IWO52_002925"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0357118.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            4682..5248
                     /locus_tag="IWO52_002926"
                     /pseudo
     CDS             4682..5248
                     /locus_tag="IWO52_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            5667..6140
                     /gene="dfrF"
                     /locus_tag="IWO52_002927"
     CDS             5667..6140
                     /gene="dfrF"
                     /locus_tag="IWO52_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0357119.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6267..6923
                     /locus_tag="IWO52_002928"
     CDS             6267..6923
                     /locus_tag="IWO52_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0357120.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7105..7635
                     /locus_tag="IWO52_002929"
     CDS             7105..7635
                     /locus_tag="IWO52_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0357121.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            7998..>9012
                     /locus_tag="IWO52_002930"
     CDS             7998..>9012
                     /locus_tag="IWO52_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0357122.1"
                     /translation="MNDIKFIETLKQKRNACDYSQSRLALELQISRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSKYFIHEDY
                     GMFGYEEQYIYGDISVNASKDSSMGVLLELRGMGCRNLEYVLQARGIDWYSFLSCCID
                     YQGVFKRIDLAINDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTNGDTAKT
                     FYIGSKSSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAEQTIEQLVFSRNPEQ
                     TIANLILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKTKRWLERQVMPSLLMI
                     KEIE"
BASE COUNT         3096 a         1496 c         1771 g         2649 t
ORIGIN      
        1 gtgtcagacc tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa
       61 gccgaaattt cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac
      121 tttttctaat ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa
      181 caatcccatt aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa
      241 tgctgctcgt ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc
      301 taagaactgg tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa
      361 cgcaaagccg aataagagga acaaccaagg actggttttt gatatctcag catctttcat
      421 caagtaacca atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa
      481 gattttgtcc caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga
      541 gatgaacaag aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac
      601 tttcaaaatg acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa
      661 tgtgattcct tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac
      721 aacgaataca ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga
      781 aagtccctcc atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca
      841 cttttccgtt caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt
      901 cagctgtttc ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat
      961 cagtatataa gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta
     1021 ccccggcatc gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt
     1081 tttctgtgtc agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg
     1141 cttcattttc ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa
     1201 ctaactgttg gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt
     1261 tcatctaaac atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga
     1321 atgatacgct ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc
     1381 aaataagaag cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga
     1441 acggattttc ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa
     1501 gcgaagacac cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca
     1561 gctgttccaa caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata
     1621 aaatatggtt cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca
     1681 tttttataaa tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc
     1741 cataagccat ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct
     1801 tcagctaccc agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg
     1861 gctgtgcgct catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg
     1921 aataaagcac catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga
     1981 tcaactggta atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct
     2041 ctgattttaa aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg
     2101 atactgtaag gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca
     2161 acgataatta gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca
     2221 gtcacaccac ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg
     2281 atttgagaga tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc
     2341 ggttttttgg aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct
     2401 tcagtcactt ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg
     2461 accggtttta attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt
     2521 attagcttga taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta
     2581 aaaactcttt aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag
     2641 aggagacgct atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc
     2701 gatcatcgct catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac
     2761 gcacactgtg acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg
     2821 tgaacgtgga atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg
     2881 agaaacatat atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt
     2941 ctctcgaagc cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat
     3001 cgaagcacaa acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc
     3061 agtcatcaat aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga
     3121 agacgtaatc ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg
     3181 gattgaagat attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga
     3241 tgcaccgcta aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct
     3301 aaatgtccgg atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa
     3361 tggcaaaaca tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga
     3421 ctttttgatg gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac
     3481 acgagtaggg gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta
     3541 ccgaaaaatg aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa
     3601 cgatttgcgg gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc
     3661 agaaacctct caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat
     3721 ggatgtcgtc caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc
     3781 atccgttatt taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc
     3841 agattttcct gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat
     3901 catggtccca aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga
     3961 attcattacg atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct
     4021 ttctgaaatt gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc
     4081 attggattat gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct
     4141 gaatggagaa aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg
     4201 tggaaaagca atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc
     4261 aatccaagca gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa
     4321 aaacgtcttg gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa
     4381 acaaaaagaa gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc
     4441 cttcatggcc gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg
     4501 aaaaacctat taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa
     4561 gattttaaac tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt
     4621 ttcttgaaga tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt
     4681 catggttgat aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc
     4741 tcaaaaaaag ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa
     4801 agggtcaaga aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga
     4861 aactctaata aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata
     4921 cagagatatg aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt
     4981 attactcagt tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat
     5041 cgatattttt ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga
     5101 gatatttagt ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg
     5161 aaagaaccgg gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa
     5221 aaatgttata agtagatggg aaaaaggtgt ttcccaaaca aaaagatatt aagaaacagc
     5281 ccatgtttac ctgcttgcaa acaaaagaat attgctacta gactaagaga tactacataa
     5341 gtgtgttggt atctcttttt tgttgccgtg aaacagcaga ctatttcatt tctgcttgat
     5401 taccttaaaa cttattagtc tatggtttgt caagggcttg ttgcgtgaga aatgctgaat
     5461 atcacccttt acaaaacata ggctgataag taacttttca aaagcagaaa agaaatggca
     5521 atgcttataa caatatgata taatgcagat gaagacaaaa aaggaatttg tcgagctggt
     5581 tagttcgaga taattttgag tttgttaaga tgtggtgcac ttgatggaat cacaggtacg
     5641 acatcatatt atggaggatg aaatagatga taggtttgat tgttgcgagg tcaaagaata
     5701 atgttatagg caagaatggt aatataccat ggaaaataaa gggagaacaa aagcaattta
     5761 gagagttaac aacgggtaat gtggttatta tggggcgaaa gtcttatgaa gaaatcggtc
     5821 atccgttgcc taatagaatg aatattgttg tttccaccac aacagagtat caaggagata
     5881 atttagtttc agttaaatca ttagaagatg cattattatt ggctaaagga cgagatgtat
     5941 acatatctgg tggatatgga ctatttaagg aagctttgca aatagtagat aaaatgtata
     6001 tcacagaagt agatttaaat attgaagatg gagatacatt ctttccagaa tttgatatca
     6061 atgattttga agttttgata ggggaaacac ttggtgagga agtgaaatat acgagaacat
     6121 tttatgtaag gaaaaaatga attgagtaaa ttttggattt agggtgtttt cataaatata
     6181 ttgcttttta tgcatatata aattgttgta agacaaattc cagtttgtat ggggcgataa
     6241 aatcagaatt tgagagagga ttgaatatga ataaggaatg gtctgaactt aataaaacaa
     6301 tgcaggcaca aataaaaaag aaagatactt ataagagggg tattgatact ttacttactt
     6361 tacgaagtca gttaatacaa accttagtat ctttcaaaga agaattatgc agagaagatt
     6421 ttaactcaat ccccttcata aacgctgatg gttatcatag taagacgatt gcttattcta
     6481 tttggcatat tttccgcatt gaagatatag ttgtgcatac agtgataaat gaagatgaac
     6541 aagtattttt tgcaggcaat tatcaagagc gtatcaattc acctatcatt acaacaggaa
     6601 atgaactcat gaaacagcag attgcagact tttcaaaaca gcttaatctg gaagaattat
     6661 atttatatat tttcgaagta tgggaaagca ctgagaaaat gcttgaacgg ttatcttatg
     6721 atgaattgaa aagaaaaata ccgaaagaaa gaaaaggata cttagaatcg ttgaatgtag
     6781 taaacgacaa tgaaaaagca atttggctga ttgattattg gtgtaataaa gatatttgtg
     6841 gactaattca aatgccgttt tcaagacatt ggattatgca cacagaagcc tgtttgcgta
     6901 taaagaacaa gatacattca taggcaaaaa tttcagtttg tagaactaag gaaattggaa
     6961 tttaaggagg aataaagtat ggttagtgat tttataagag cagcatttcc atggatttta
     7021 atgggtttat ttgtagcaat cagttgttcc cttatgagta aaaaagagaa ataaattcct
     7081 gtttgtattt ataaaagaga aaaaatgaga aaagtagttt tatttattgc catgagcctt
     7141 gacggatata ttgcggacgg taatggtgga gtagcttggc taaatggtca tggaaatgac
     7201 aatgaaaaca tcgacactta tacagaattt accaaagata tagatactgt cttaatgggg
     7261 tggaatactt ctcatcaggt tgttactgaa ctttctccgc aagaatgggt atataataaa
     7321 tttacaactt atgtattaac acataaagag tgcaattctt cccaacaaat tcattttaca
     7381 agtgaaaatc ctgttttatt attggaacgg ctaaaacaag aagcaggaaa ggatatttgg
     7441 atttgtggcg gagcaaatct tgttcagcag ttggtaagca aaaatatgat tgataaatat
     7501 tacatttctg ttattcctac tctgttagga aatggggttc gtcttttggg caacatagac
     7561 agggaaataa agttaaggct ttgtaaaaca cagacttata acggaataac cgatttgatt
     7621 tatatgcgta gataactaga gagaaacaga aaagctgtta aaccgacgga gcgatccgac
     7681 cacgttggcg ggaaaatcct taagattttt ccgccatgct tgcaaggggg cggttcgctt
     7741 cggcggttcg gcgagcttgc gagccgttca aagcccccgt tatctaacag gggggtacta
     7801 ttacaaaaga caagagcaaa aacaaataca aaactgctga tatgcaacga tttataaagt
     7861 tataagaact tatttcactt cacaccctat tctttttacc cgacaggtct aaaacagcat
     7921 atcaatagaa tataaatata agatacaagg tgtatgctcc tatgagtata tgcctttttt
     7981 gattggagga ataaagcatg aatgatataa aatttattga aacattaaaa caaaagagaa
     8041 atgcctgtga ttattcgcaa tcacgactgg cactggaact acaaatcagc aggcagaact
     8101 taaatgaaat tgaaaacgga aaaacaaaag ccagtaaaga aatgaaacat atacttctac
     8161 attatcttga ttattgtaat tgtacacagc ctttcacttt aacgattgac tatctgcgtg
     8221 ttcgttttcc taccacagac gcattggaga ttataaaaaa tgtactggca atgaaaagca
     8281 aatattttat acatgaagat tatggaatgt ttggttatga agaacagtat atctatgggg
     8341 atattagtgt aaatgcttct aaagatagtt ctatgggtgt tttattagaa ttaagaggta
     8401 tggggtgtcg gaatttggaa tatgttttac aggcaagagg gatagactgg tattcctttt
     8461 taagctgttg tatagattat caaggcgttt ttaaacgtat agatttagca atcaatgata
     8521 tgggcggatt gctggatata gaaattctaa gggaacgcta ttatgccaac aaggtatgga
     8581 aacgttcaag aacccatgaa gcagtagaca gcggaaaatt atcgggtaca aatggagata
     8641 ctgcaaaaac tttttatatt ggttcaaaga gtagttctat ttacttttgc ttgtatgaaa
     8701 aagaaaagga acaaaaaagc aagggcataa aaacagacat taagaaccgc tttgaaattc
     8761 gtttgaaaaa tggaaaagca gaacaaacga tagaacaact tgttttttca agaaatcccg
     8821 aacagaccat agcaaatctt atcctcacac aaatagattt tcccgattat attttatggg
     8881 atatattttt agataatgta acgacttcac ttccatttat tatgacacct gttgctgtca
     8941 atatggataa aacaaaacgc tggttagaaa gacaggtcat gccctcttta ttgatgataa
     9001 aagagattga aa
//
LOCUS       DACEDO010000206         8291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.guided.097, whole genome shotgun sequence.
ACCESSION   DACEDO010000206 DACEDO010000000
VERSION     DACEDO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.guided.097"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            258..2144
                     /locus_tag="IWO52_002931"
     CDS             258..2144
                     /locus_tag="IWO52_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0357123.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2156..3103
                     /locus_tag="IWO52_002932"
     CDS             2156..3103
                     /locus_tag="IWO52_002932"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0357124.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3123..3650
                     /locus_tag="IWO52_002933"
     CDS             3123..3650
                     /locus_tag="IWO52_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0357125.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3713..4366)
                     /locus_tag="IWO52_002934"
     CDS             complement(3713..4366)
                     /locus_tag="IWO52_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0357126.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4498..5340
                     /locus_tag="IWO52_002935"
     CDS             4498..5340
                     /locus_tag="IWO52_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0357127.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5498..6394
                     /locus_tag="IWO52_002936"
     CDS             5498..6394
                     /locus_tag="IWO52_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0357128.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6397..7110
                     /locus_tag="IWO52_002937"
     CDS             6397..7110
                     /locus_tag="IWO52_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0357129.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            7130..7648
                     /gene="msrA"
                     /locus_tag="IWO52_002938"
     CDS             7130..7648
                     /gene="msrA"
                     /locus_tag="IWO52_002938"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0357130.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7645..7872
                     /locus_tag="IWO52_002939"
     CDS             7645..7872
                     /locus_tag="IWO52_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0357131.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            8024..>8291
                     /gene="lepB"
                     /locus_tag="IWO52_002940"
     CDS             8024..>8291
                     /gene="lepB"
                     /locus_tag="IWO52_002940"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0357132.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENV"
BASE COUNT         2981 a         1433 c         1683 g         2194 t
ORIGIN      
        1 atttgttaga atcttatacg atgaacaaac catctgataa cttttctttc cgatttgcgt
       61 ttcgagcaaa gtcaaaaaaa acgagtccaa taaaataaaa aggtaaaaat tttcaagttt
      121 tataaaaaac aaaacatatc agaacaggat cgaatcggtc ctgttctttt tttgagaaca
      181 caaaaagaac aaataccaaa aaaattttcc tgaagttcac tatttttatg atacaatatc
      241 caagataagg agggaacatg ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg
      301 gtgaaaaaga tttgtttgac cagatttctt ttttggtcca cgaaaaagac cgtatcgggt
      361 tgattgggac gaacgggacg ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata
      421 gcagcgacgg tgatcggcaa acagtgtttt atccaactga ttaccgtatt ggttatcttt
      481 ctcaagcagc tgaattttcc gatgaattaa ctgttctcca agcagtcttt caaggaaaca
      541 gccccttgat ccaaactgtt cgagcatatg aggaagcatt gatcgaacta ggggaaaacg
      601 gagaagaccc tgatgtgcaa aaacgttatg ctaaagcaga agaacaaatg aataaagaag
      661 atgcttggac tacagatacc aacgcaaaga ttattttgca aaaattagga atcagtgaat
      721 tagataaaaa aatcagtact ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag
      781 tgttgattga tgaacccgat ttactattac tagacgaacc aacaaaccat ttagattatg
      841 aagcgatcga gtggttggaa aactatttga aacaataccg tggcgcacta ttgatggtta
      901 cccacgatcg ttattttcta gatagagtag caaaccgaat cttcgaactt tcatttggaa
      961 aattatacga gtataaagga aattatgaag cttacgtctt agaaaaagca gaacgtgatc
     1021 gtgtagaagt agaacaagaa gaaaagcgca agcggcttta taaacaggaa ttagcttgga
     1081 tgagggctgg cgtacaggca cgtggcacca agcagcaagc acggatcaac cgtttcgaag
     1141 atctaaagga gaacttgtac caagtaaacc aagaagatga tttagagctg aacctagcga
     1201 cacaacgttt agggaaaaaa gtactggaaa tcaaagatgg cagttaccgt ataaatgacc
     1261 aaacactgct agaacattta gatttattga ttcaatctcg ggaaagacta ggtattactg
     1321 gtaaaaatgg tgcgggaaaa tcgacattgc ttaatattct agctggcaga attcccctgg
     1381 atagcggtac aatgtctatc ggtgaaacgg ttcatcttgc gtattataca caagaaaatg
     1441 aagaaatggc accagataaa cggatgattg cttacttaca agaagctgct gaagaagcaa
     1501 aaacagcaga tggcagtcaa atcggagtag cagaattact tgaacgattt ttatttccaa
     1561 gatttatgca tgggacactg ataggcaaac tttcaggcgg agaaaaacgc cggttatttt
     1621 tgttgaaact gctgattcag caacctaatg ttttattatt ggatgaaccg accaatgatt
     1681 tggacatagc aacattgacg attttggaag attattttag aagttttcca ggtgctgtga
     1741 tcactgtttc ccatgaccgt tatttcttgg acaaggtagc agataagtta ttagtattcc
     1801 aaggaaacgg aaaacaagaa ctttattacg gaaacatgtc cagttattta ctgaagcaaa
     1861 aagagaccca gcagcctgct gaaaaagcga aaccaaaaat acagagcaag gaacctgctg
     1921 aaaagaagaa attatcttac atggaacaaa aagaatggga aacgattgaa gatgagattg
     1981 cagaacttga agaaaaaatc tcattgctcc aagaagagat gaatcatcaa ggagataatt
     2041 tcactcgatt acaagaattg caaaacgacg tatctgaaac agaagcacaa cttgaagaaa
     2101 aaatggcacg gtgggaatac ttgagtgaat gggtagaaga ttaggaggaa aaaggatgga
     2161 acaagcgtat cttgacttag gaagaaaatt gttggaagaa gggcatcgta aagaagacag
     2221 aacaggcaca ggcacattaa gcttattcgg ataccaaatg cggtttgatc tgcaaaaagg
     2281 gtttcctttg ttaacaacga aacgagtacc ttttggattg atcaaaagcg aattgctttg
     2341 gtttttaaaa ggagatacga atatccgcta tctattacaa aataaaaacc atatttggga
     2401 tgaatgggca tttgaacgct acatcaaaag tccagactat aatggaccag atatgactga
     2461 ttttggccat agatgtttga cagatcctga attcaatgaa gaatataaaa agcagagcaa
     2521 gcttttttgc gagaagatcc taaccgatga ttcctttgct gaaacatacg gcgatcttgg
     2581 acacatctac ggctatcaat ggcgtcactg ggaaacaaaa gacggtggct ttatcgatca
     2641 aatcaaacaa gtgatcgaag caatcaaaaa gactcctgat tctagaagat tgatcgtttc
     2701 tgcatggaat ccggaagatg ttcctagcat ggctttgcct ccttgtcata cgatgtttca
     2761 attttatgtt caagagggac ggctaagctg ccaattgtat caacgcagtg gggatgtgtt
     2821 tctaggcgtt ccgttcaaca ttgcaagtta tgcgttgctg actcacctaa ttgcacatga
     2881 aacaggttta gcggttggcg aatttgttca cacattagga gatgctcatc tttatatcaa
     2941 tcatatcgaa caaatgaaag aacaattgag ccgagaaata cgcgcatttc cgactttagt
     3001 tttgaatcag gaaaagcagt cagtatttga ttttgagatg gaagatatcc aattagaagg
     3061 atatgaccca catccggcga tcaaagctcc tatcgctgtt tagaatcaga aaggatgaga
     3121 aaatgttagc tgcaatttgg gcacaggatg aaaacggatt gattggaaaa gaagaccgtc
     3181 ttccttggcg tttgccaaat gatctgaaat tcttcaaaca aatgacagaa gcaaatacac
     3241 tagtgatggg acgaaaaaca ttcgaaggga tgggtagccg tccgttgccg aatcgtcaga
     3301 cgattgtttt gacgagagat tcttcctata aagcagaagg cgtgcatgtc atgcatagtg
     3361 tggaagacgt gcttgctctg gaaaaagaaa cagacggtat ctttttcatc gctggagggt
     3421 cagctgttta tcaagaattt ctgccatttt gtacgatatt ataccgtaca gtcatccatc
     3481 atgtattcga tggagatgct tattttccac ctgttgactg gtctgattgg tcactgatca
     3541 atactagtca aggagaaatg gatgaaaaaa atcgttatcc gcatcaattt gaaacgtatc
     3601 agcgtaatga gaatcctgta aaaagcggga caggcgaatt gacgatataa aaaagctatg
     3661 gatagactag ctgttattta gagctgtccc atccatagct ttattttttt tattaagtaa
     3721 agaataaaac agagaagtac ataaaacctg cacctaacat aacaaacaaa tgccaaacaa
     3781 catgcatata gcggatagaa ttcaagctat aaaagaatgc gcctatcgtg taagaaagcc
     3841 cgcctgctac catcaaaata acaccagtca ttccaagaga gtgataaaga ggaacagcag
     3901 ccacgacaca gagccacccc ataaaaatat agatgatcgt agatactttt gatactctct
     3961 cccgtttatg caacgtcaac gctttatata taatgccaag aatcgcaaac aaccagatta
     4021 atacaaataa agtccaacct agccatcctc ggatactcag caaacaaaaa ggagtataac
     4081 ttcctgcaat caaaagaaaa atcgaactat gatcgaatac ttggaacacc ttttttgctt
     4141 ttgtaaaaat caatgaatga aacaaagtag acgttaaaaa taaaagaatc atcatcgatc
     4201 catagatagc ataagagaca atatggaggg aggaatgcaa gtgggcacct tttactaaaa
     4261 gaaccactag tcccgcgata ctcaatccag tcccaattcc atgggtgact gcattaaaca
     4321 cttcatttaa tatttcatac gttcgattaa atttcgtttt ttccatgaaa acggctcctt
     4381 tactaggtgt aattaatgaa aaatcattat ttgtctgtta tactaaaaat agaaattgtc
     4441 ttacatgtag tttaacatga ataaggcatc ttgttaaaga aagagagaga gaaaataatg
     4501 acaaatgtaa aaatcgtaac agattcttca tgtacgatgg aaaaaaatgt acgcgatgct
     4561 ttaggcattc gtgtcatgcc gctatccatc atgatcgacg gaacagtata tcccgatgat
     4621 gaccaactag acggcgaaag atttatggaa atgatggcga aagcaaaaaa tctgcccaaa
     4681 acaagccaac caccaattgg agaattcatc tcgatgtatg atgagctagg agaagacggc
     4741 agtgaagtga tttcgatcca tatgacaaaa ggcttgagtg gaacagtaga agcagcaaga
     4801 caagccagta atctaacaaa aacgaaagta acggttatcg acagtgatac cactgaccaa
     4861 ggattatctt ttcaggtaat tcgagcagct gaattagcaa aagaaggtaa aacagtagag
     4921 aaaatccttg aagaagtcaa aaaagtacaa gacaatacaa aattatacat cggaatatcc
     4981 acactagata acttggtaaa aggtggaaga atcagccgaa caactggttt gctgtctagt
     5041 gttttgaaca tacgcgtggt gatgaatttc gcccattctg aattaatccc tgtaactaag
     5101 gggcgaggaa agaaaacgtt cgataaatgg tttaataatt tgaaaaatga actgaaagaa
     5161 ttgccaaatg tgcgacaaat cggtatttca catgcggatg caaaagaatt ggcgcaagaa
     5221 ttcaaagagg gtttgcaaga attattcccg gacatgcata tccctgtact gcatacgaat
     5281 ccggttatcg ctacgcatac tggtaaagac gcgtttgcaa tcatgtatta tgtagattag
     5341 tcgaaaagag gataagccct cagcctgaaa ggcttgcagc ttattctctt tttctttata
     5401 aaattaggaa aataattgtt ttcttttttc ataaagacta cctttttgat aaaatttttg
     5461 ataacattag aaccaatcga atacaagagg tgaaaaaatg aaattaacga aacgacattt
     5521 gatcctcgtt ttgttcccat tattaattgc gatcatcatc tatgcaggtt tagctgcttc
     5581 tataccgaaa gctggcccac taatgaaacc agtaactact gtgagagaag atccaaataa
     5641 aaaagaaaaa cttcactaca tagctatcgg agattcgctg actgaaggag taggagatca
     5701 aaccaagcaa ggaggattcg ttccccttgt cgcaaatgat ctaaaagacc gttataattt
     5761 gaccgcggtt gaaacagaaa actatggtgt aaatggtgaa cgaagtgacc agatcttaaa
     5821 gcgagtaaaa acaaaagaag aaattcgaaa caatatcaaa acagctgatt ttatcacact
     5881 gactgttggc ggtaatgatt tgatgaaagt tatccaaaat aatttattcg ggttaacggt
     5941 caattccttt aaaaagccac tgaaaaacta tcaggaaaat gtcacagaac tattggaaga
     6001 atttcgatcc ctcaataaaa ccgcacctat ttacatttta ggcatctaca atccatttta
     6061 tttgaacttt cctgagatca ctgacatgca gacaattgtt aacaattgga acaaaggaac
     6121 agaaacggtg acaaaaaaaa tgccgaactg ctactttatc ccaatcaatg atttgcttta
     6181 tcaaggattg aatcaggaag taggtgtcac agaagaagga acagatcaaa caactagcac
     6241 gacagaaaat ctaaatggca tcaaaaataa tgtgctctat gaagaagatc atttccatcc
     6301 aaataattta ggttatcaat tgatggcaaa tgcagtgaga gatcagctga tcgagacaca
     6361 aaaccaatgg ttagagaagg aggatgatca ataaacatga aaacaagatc tgacgtatct
     6421 tcaaacaaaa caaagaaaac aaattactgg aaattcgcct tccttatcct tcttggggta
     6481 gtacttggtt cgaccgtatt tttaggcagc cgtatttttg ctaatcggga accagaactc
     6541 ccagaaatac cagctcttac ggaaagacaa ggagatcctg tcttaacgat caattcaaat
     6601 aaagaaaaag tcaatcaaat catcagcttt ttccttagtg agtatcaaaa agatagtgat
     6661 attgaatata aattttattt ggaaaatgaa gcactactca atggtacttt cgaagtacta
     6721 ggttttccta tcgatttcta tttatatttt gatccgtatg tgatggaaaa cggaaatgtc
     6781 caactaaaag ctagaagtct ttctatcgga acattgagtt tgccaatcaa agatgtgatg
     6841 aatatgatca agagaaacta taaattacca gattggatcg aagtcaatac agatgatctg
     6901 actatcatgc ttaggttgga tcaattccga atgcaaaacg ggatgtatat taaagcagat
     6961 aaaatcgact tagtaaatga tgacattcgg tttagtctat atttgccaaa agatacctct
     7021 gatgcttcaa acaactcaaa taccaacaaa actagcacca gcagtactga aagcaagaca
     7081 aaagaaaata aaaaatcatc ggatgagtag tattagaggg ggaatcataa tggaacgagc
     7141 aatatttgca ggaggctgct tctggtgtat gatccagcct ttcgacacgc ttcctggtat
     7201 ccatacagtg atgtcaggat atactggagg gcatgtacca gatccgactt atgaacaagt
     7261 aaaaagccaa acgactggac atacagaagc agtggagatt atctatgatc caacattgat
     7321 ttcatatgaa caattagtag aactttactg gcagcaaact gatccaactg acgctttcgg
     7381 acaattcgag gatcgcggtg acaattatcg cccggtgatc ttttatttga cagaagaaca
     7441 gcggatcatt gctgaaaaaa gtaagaaaca gttagctgaa agtggacgtt tcgaacagcc
     7501 aatcgttaca aaaatcgaac cagcccaacc attttatcta gcagaagaag accatcaaga
     7561 ctattataaa aagcatcctg aaaattttaa aagaaaccat gcacgccggg ctgcctttat
     7621 cgaagaaaat tggaggaacg aagggtgaat cgaagctttt ttcattattt aatgacactg
     7681 cgaggcggtg gcacacatga tgctttatct gttttcgcta cagaagccgg taaagacatc
     7741 caatttccta agcacagctc tagctacgaa gaaatttcta attatctgga actgaatgtc
     7801 gactatttgc cgtccatgga tatctttgac gaagcatggg aaaaatattt agaaaataat
     7861 catgacatat aagagatagt tttgtacttt atgatcaaaa aagttttttg tccaatagcc
     7921 taattgaaag aattattttt atttcagcta tctagtcgca tttttttgat atttttctta
     7981 taataaagaa agaatgaaaa aaagagggat ggattgatag agattgacaa aaaaacaacg
     8041 ctatattaat cgcttctggc ttgtattcaa atacttactt gtttctgtat tccttgcgtt
     8101 catgttgcgg ggatttttat tcatcccggt accagtggaa ggcaattcaa tggaaaacgt
     8161 gttgaagcaa ggtgatatgg ttgtgatgga aaaattttcg gagatcagac gatttgatat
     8221 tgttgttttt caattagcag atggcacgat ttatatcaaa cgagtaatcg gtcttccagg
     8281 agaaaatgtc a
//
LOCUS       DACEDO010000207         7577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.guided.103, whole genome shotgun sequence.
ACCESSION   DACEDO010000207 DACEDO010000000
VERSION     DACEDO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.guided.103"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..272)
                     /locus_tag="IWO52_002941"
     CDS             complement(<1..272)
                     /locus_tag="IWO52_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0357133.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFT"
     gene            554..1450
                     /locus_tag="IWO52_002942"
     CDS             554..1450
                     /locus_tag="IWO52_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0357134.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(1593..2534)
                     /locus_tag="IWO52_002943"
     CDS             complement(1593..2534)
                     /locus_tag="IWO52_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0357135.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2660..3781
                     /locus_tag="IWO52_002944"
     CDS             2660..3781
                     /locus_tag="IWO52_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0357136.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            4039..5541
                     /gene="lsa"
                     /locus_tag="IWO52_002945"
     CDS             4039..5541
                     /gene="lsa"
                     /locus_tag="IWO52_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0357137.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5608..6135
                     /locus_tag="IWO52_002946"
     CDS             5608..6135
                     /locus_tag="IWO52_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0357138.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            6347..7408
                     /locus_tag="IWO52_002947"
     CDS             6347..7408
                     /locus_tag="IWO52_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0357139.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7411..>7577
                     /gene="murQ"
                     /locus_tag="IWO52_002948"
                     /gene_synonym="yfeU"
     CDS             7411..>7577
                     /gene="murQ"
                     /locus_tag="IWO52_002948"
                     /gene_synonym="yfeU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="catalyzes the cleavage of the lactyl ether moiety
                     of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form
                     N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate;
                     involved in MurNAc dissimilation pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0357140.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIA"
BASE COUNT         2531 a         1337 c         1577 g         2132 t
ORIGIN      
        1 gtaaaatcgg cagcatgagc acctagatat ggacgaatca atccagataa tccgtggtgc
       61 gtatcaccac cacttaaaac aatttttccc agacctttca tcacgatata taaaattatg
      121 ccgccaatca aaaaagcgat caatacagaa gggccggcga ttccaatcgc ttcgccactt
      181 cctttaaata gacctgcacc aatcgctcca cctaaagcta gcatggtgat atgtcgggag
      241 ctaagttgtt ttttcaattt tttatcttcc ataaataacc tctttctttt aaattcacaa
      301 tcaaaatgca tagtaagaat aaatagtaag aataaatagt aataataata atgactaatg
      361 ataacaaatt ttcagataat tacaactatt cagaaaactt ttgggcatat tttaatggaa
      421 gaatgattta tttcatcgta ttttcataga aatgttaaat ttttataaaa ataataggaa
      481 gcgttttact ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag
      541 tgaggcggaa cagatgaaca aaaacgacgt attgctaatt gcgaaaaata aatcacttta
      601 tttttttcct tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga
      661 attcattaaa tcagctcgac aacagttggc aaattatcag ctgatcgttt ttttagatta
      721 tggatttgat atggagatgg ctcgacgcgt gcgtccattt accaatgcaa aaatcgtgtt
      781 atttttttgg aaccacttta aagcagaaca cgaggcgatg ctgaagcagg cacaagatga
      841 accggcaatc gatgagattt atcattttga ttttttagaa gcgaaagaac ttggactaaa
      901 acataatagt tccttttatt cgaaacatat ggatttttct tctagcgaac caaagacgga
      961 tctatttttt ggtgcaacaa ataacggcag aaaagaacgg gcagaaaaat ataagcagga
     1021 atttacaaaa agaggtgtta cgaccaatta ctttattctt ccgcttcgag gaaatgaaca
     1081 acctggatac cttacttatg aagaatatct gaaaaaaacc ggggaatcga gggggattct
     1141 agaactgttg cgggaagggc agcaaggagt gactttgcga acgtttgaat ctatgtattt
     1201 tcaaaagaaa ctagtgacag ataatcaagc catagctcat tatcagtttt atcattctga
     1261 aaatattttt ttgcttcaag aacgtgattt agatgaatta ccggaatttt tgcatacgcc
     1321 atttcaacca atcccatcag aactgttgga attttttgat gcagaaaatt gggcaaaacg
     1381 ttttttcact actgactccg ctatttatga acaatatgaa tacgatcttt cctctgttaa
     1441 aaataaataa aagtgccaac caatcataga tgaaaataaa caagcgcaat tcttatggca
     1501 acatgagaat tgtgtttttc tttttgaact ttcatggggg taaaaaaatg agaaccagct
     1561 gctgatccag ctgattctca tcagagagcc ttttattctg cgccaaggat tccttctttt
     1621 gcatggacta attgggtcaa aaaggcacaa tatggtgtag caatatcata tttttgtcct
     1681 tttcgccaaa cggcaccatt gatgtagtcg atttcagtca aacgatggtt tttgatcagg
     1741 tcttggtaca tagatgggta atgcaacccg atgttgtcag gatcataagt agaagcaata
     1801 tgttggtaca cttcttcttg atctaatgtg attccttctt ttgctgcgat agaagcaaac
     1861 tcagatacaa tcgttcgaac catgccctct gccgcttttt gcgcacctaa ttcagcaata
     1921 ttacaatcca atatcgtgca taatccattt aatgttccgt ttacacaagc ttttcgccaa
     1981 atggaataac gaacattatg actatagatt ggattcaatt ttgcttcttg aaagacttct
     2041 acaacttttt ttgtaaatgc ttcgccttct ggttccaagt tctctagttc gatttctcca
     2101 tcacctaata atgtgacttt tccgggacca gctagaccag ctgtccacat cgtgatccca
     2161 aataagatat ttttctttgg tacatatttt tccagtacat cttcatgacc taatccattc
     2221 agtaaacaca aaacataagt attctcagta ataatggatt ggatcgaacg aaacatatca
     2281 tctaattgat tcgcttttgt caaggctacg atcaaatcca cgtgtttgtt gctttcgatg
     2341 atttcttctg gtgaatagat tggcagtttt gcaactactt ctttgccatt aaagtcagca
     2401 atcaatccat ttgtacgaat ggcttcaatg tgagcaggcc agcgatcgat caagacgacc
     2461 tcatttccgc tttgatgcaa cattaatccg attcggctgc ccattgctcc ggctccagct
     2521 attgcgattt tcatgttttt tctctccttt attcaatgag ttccctttta gtatatagaa
     2581 aaacagaaga aaatagcatt gttttgtttg aaaaaagtga aaaaaagtgt cttacttaag
     2641 aagaaagtga gggatataga tggatatttt ggcggcaatc gattcattta aaggaagtgc
     2701 ttcttcattt gaattaaatc aggcagttct aaatggttgg gaagaccgta aaggaagaaa
     2761 aatcaatgtg ccgattgcag atggcggaga aaatactgca gaagcaatct attacgcgct
     2821 cgggggaacc tggcgtacga ttgatgggat ggatctctta tttcgtcagc gaaaaatcag
     2881 ttatttattg acgaactggc aaggaaaaaa taccgcagtc attgaatctg cacaagtatt
     2941 aggattagat ttacttgata aaccaacgga tcaaaccatc cgacttgctt ctagttatgc
     3001 cttgggagag cttatcaaag atacactgct tcaagaagtg gaacaaatca ttgtcacttt
     3061 aggaggaagc ggatgtagcg atggtggtct aggattactc caatctttgg gtgcttcttt
     3121 agaagggtac acagaaggaa atcctttgtt atctactaga aaacttcatt tggatcgaag
     3181 gaacgttctt gtttctaaag taaaagtgat catcgctgca gacgtgacca acccatatac
     3241 aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa ggaggaacgg atacgacgtt
     3301 agctttcttg gataaacaag ctgaaaagat tgctcagcag ataaaagatg tttgggaggt
     3361 ggatttagat gacatcccag gaagcggagc agcaggcggt attggcggag cccttttttt
     3421 gttaggagcg aagatggtct ctggttttga gttggtcagc caaatcactg gattagaaaa
     3481 acagatcgaa caagcggatg tcatcattac aggagaaggg cggattgatc agcaaacaat
     3541 ccacggaaaa gttccatatg gcgtagcagt gcttgcaaaa aaatatcgaa aaccaataat
     3601 cgctatttgc ggaagtagag accgagagct ggaaggactc tttgattttc tccccattgt
     3661 cttaagcatt caattaggac caatcgattt agaggaagca atggaacaca ggcaaacatt
     3721 agaaaaaaca gaaatcttag gacagacact cagcagattt ttcaacattc tcccgaagta
     3781 gttgacaaaa acaatttctt tatgctatcg taatttcaaa tataggacaa agtgagggat
     3841 ccgtgcattg aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag
     3901 acaagggagc agtgtgcaga ttttcgggat tggcttggtt ctttcgatgt atgggtcttt
     3961 ttgtgtgccc caacccttgc atcctcttta gccagttctg aaaataggac tggtcttttt
     4021 ttacgaggtg atggaataat gtctaaaatc gaaataaaaa atctgacatt cggctatgac
     4081 agccaaggca cattattatt tgaacaagcc aatctaaact ttgacacaca atggaaacta
     4141 ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatatcct gcaaaacaaa
     4201 ctcccttatc aagggcaagt gatccatcag caagcgtttg tctatttccc gcagcaaaca
     4261 aaagataaag agcgtttgac ctattatgtg ttaaatgaga ttatggattt tgagatatgg
     4321 gaaattgaaa gagagctcca actgatgcaa acagatcctg aaatcttatg gagagaattc
     4381 agcacactat caggaggaga gaagacaaaa gtcctactgg cactcttatt tgtggatgac
     4441 actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg cagaaaacaa
     4501 gtagcggctt acttgaaaaa gaaaaaacaa ggattcatcg tggtgagtca tgatcgggga
     4561 tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa agagccaact ggaactttat
     4621 caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatagct
     4681 aaaaatgaaa aattgaaaaa agaagtcagc aggctaaaga aaacagcagc tgaaaaagca
     4741 gaatggtctc gttcccgaga aggggacaaa acaaagaaaa aagtcggatt catcgatacg
     4801 gaatctaggc gagtgaataa aggagcagta ggcgctgatg ctgcacggac gatgaaacga
     4861 tctaaagcaa tcgtgaatcg gatggaaacc cagatcagtg agaaagaaaa attattaaaa
     4921 gatatcgagt atatcgatgc tttgacgatg aatagccaag tgtctcatca taaacggctg
     4981 ttaagcgtag aaaatcttca attagggtat gaaaatctgt tattcgagcc agttcatttt
     5041 acgatcgaac ctcatcagcg gatggcaatt tcaggtccta acggcgcggg aaagtcatcc
     5101 attatccatt atcttctggg gacattcaac ggcaaggttg taggagaaaa atcccagccc
     5161 aaacatttga ccattagtta cgtacgccaa aattatgaag acaatcgagg gacattggcg
     5221 gaattcgcga agaaaaaccg agtagactat caagcatttt tgaataatct ccgaaagctt
     5281 gggatggaaa gagatgtttt tcataataaa atcgaacaga tgagtatggg acaacggaaa
     5341 aaagtggaat tggctaaatc tttatcccaa ccagctgagc tctatatatg ggatgaacca
     5401 ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatttt gaacgtgaaa
     5461 cctgctatgt tactagtgga acatgatcaa acctttctag ataaagtatc tactgagatt
     5521 atttctcttg agaaaatcta aaccttgtaa tctcaaagac aacaaggtat ggttaaggag
     5581 aactttgtca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact
     5641 tggaacgcac aaggattagt ttgcgggcga gcagatgtgc cgctgactga aaaaggacag
     5701 atgcaggcac aggcactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc
     5761 atccattccc ctcttcaacg agcaagagat acagcacaag cagtagttga tcgtctcaat
     5821 ttgccgatga ccgctgatga gcgattggta gaaatggatt ttggggatta tgacggactg
     5881 ccaagcaaag atgaaaactt tcaaaaagcg cgtttagcgt ttgctgtacg tttcccaaat
     5941 ggagagtcgg tcttagatgt gtacgcacgg atcgttcctt tattgaaaga atgcatggaa
     6001 gacgaagaaa atgtctactt gttagtctgt cataatgcct tgataagagt gatcaatgct
     6061 tattttcatc caatgcctaa tgaagggttc tttacattca tggtagacaa tacagaatta
     6121 atttcttatg agtaattaga cttttgtgtt atttaaataa aatgtttcaa ggcactgctt
     6181 ttgcttagca gtgccttttt tgtggctgtg tcaattcttt tggcattata aatccaattt
     6241 ttagaaataa aatttaatta ttttaacaaa agtattgaaa ttttgtttct ttagatatat
     6301 actctagtca tagaaatgaa gacggataca gaaaaagagg gataatatgt ttggcttttc
     6361 aatatttatg aatgattctt tgtcagaaga gaagaaacag tatatccgcc aaatggcatc
     6421 caatggcttt gtcggtattt tcacttccat gcatataccg gaagatgatg tttcggctta
     6481 tcaaaatcga ttgatcgaac taggaagcga agcgaaaaag aataaactag agttgatggt
     6541 cgatatttca ggagaggcat tgaaacgagc tggcttttca ttagaagatc taacaccctt
     6601 gaaagcaatt ggtgtaacag ggttaagaat ggattatcat atttctaatc agcagatcgc
     6661 cgactggtca catcaattga aaatcagttt gaatgccagc acgatcacgc ctaatgatat
     6721 cgatgagttg aaagaagcag cggctgattt ctcacagatt gaagcttggc ataattatta
     6781 tccacgtctt gaaacagggt tggacaaaga atggtatcag gaaaaaaatc aatggctcaa
     6841 aactcaagga ttccttgtac aaggctttgt accaggagat acagagttga gaggtccact
     6901 gtatcaagga ttaccaactt tggaagaaca tcgagggatc catcctctgg caggtgctct
     6961 tgatctatta gcaagtaaca cggatattgt ttatatcgga gatgcaggtc tgtcagaaca
     7021 cgttcaggag caatttgctt ctttccaaaa agaacagaca gtcttgctcc atacggaacc
     7081 tgtagatgaa gttttttacg aatatatatt aggaaaacat acgaatcggc aagatgatgc
     7141 aagagatgtc atacgaagcg cagatgcgcg ttttcgagag attccgccaa tcccagtaag
     7201 aaatacagct aagcgtatga aaggatctat tactttagat aacgaaaagt atttgcgcta
     7261 tatgggtgag atccaattaa ctaaatatga tttgccagcg gatgaaaaag tgaatgtagt
     7321 agccaaagtc atcaaagaag aactaccgct gatcaaccaa atcaaagctg gtatgaacta
     7381 tcaatttata agaaaagaag gaagataaaa atgagcaaaa tcaatttaga gaacctaaca
     7441 acggaacgca gaaaccaaga aacttttgga ttagatgaaa tgagtatttc tcaagcagtc
     7501 caactcatga acaaagaaga tcagaaagtg gccgctgctg ttgaaaaaga actgccaagt
     7561 atcgagccag tgatcgc
//
LOCUS       DACEDO010000208         6257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.guided.112, whole genome shotgun sequence.
ACCESSION   DACEDO010000208 DACEDO010000000
VERSION     DACEDO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.guided.112"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..2498
                     /locus_tag="IWO52_002949"
     CDS             51..2498
                     /locus_tag="IWO52_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0357141.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            2728..3795
                     /gene="rlmN"
                     /locus_tag="IWO52_002950"
     CDS             2728..3795
                     /gene="rlmN"
                     /locus_tag="IWO52_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0357142.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3988..5091)
                     /locus_tag="IWO52_002951"
     CDS             complement(3988..5091)
                     /locus_tag="IWO52_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0357143.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5644..6192
                     /gene="aac(6')"
                     /locus_tag="IWO52_002952"
     CDS             5644..6192
                     /gene="aac(6')"
                     /locus_tag="IWO52_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0357144.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2083 a         1077 c         1347 g         1750 t
ORIGIN      
        1 tcccttactt ttttttgtat aatgaaactt gatgaaatga gggataataa atggcacaaa
       61 agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc
      121 aggaacaaat gctgacgatt ggtgttgggt ttgccttgat tttatttgct gtcttcggct
      181 ttttaaaatt aggtttttta ggaatattga ttgcaaacgg ctttcggatc attgcaggaa
      241 atacttacca aatcctctgt cttttattag ccgtattagg attttggatt gtcattaaga
      301 atacagagtt ttcaattggt aaaaaccgac gctggtttgg cggcatactc ttttatttcg
      361 gtatcttatt attgttacat gcccatttat ttggcaaact ccatactggg gaaccaaata
      421 tcatggggac gacatgggat ttgttagcca gtgatataaa acaaagtcaa gtagacaata
      481 acgttggtgg tgggatgatt ggagcaatcc tttatcattt tacttacttc ttgattgcac
      541 agcctggaag ttatttagca gcagtgctat tactggcagg aggagcattc ctgttcagta
      601 atctggaagg ctatcagctg ttaaatggta tccaatcaat tggagaacga gtacaggaat
      661 tattggaagg tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga
      721 aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag
      781 aagctgcaga atatgagaag aaaaaagcgg tgagccagca aagggaaaat actgctgatg
      841 aatggcagga accagaaaat acagagccgg agcagctaag ttttgttcca atcgacagtt
      901 ttcaagaaaa tatccatcct gcgaatctag agaaaccagt accagataca ccaaaacaga
      961 ccaatacagc agaaggtttt tcagatgagt tgccagagga tgacggtacc tcattagaat
     1021 ttgagattga agcagaacag gaaaatcaag actatgaatt accgacagtt gatttgcttg
     1081 attctatacc aactgtggat caaagtgatg agtataaaaa aattgaaaaa aatatcggcg
     1141 tattagaaca aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag
     1201 gaccatccgt cacaaaattc gaagtccagc cggctgtagg ggtaaaagta agtaaaattg
     1261 tgaacttaac agatgatatc gcgcttgctt tagcggcgaa agatgtccgg atggaagcac
     1321 cgattccagg taaatcactg attggtattg aagtaccaaa tggaaagatc agtatggttt
     1381 cctttagaga aatcatcgaa gcacaaccaa accacccaga taaattacta gaagtacctt
     1441 taggacgtga tgtttctgga cgggtacaaa cggctgatct ttcgaagatg cctcacttac
     1501 tcgtggctgg ttcgactggt agtgggaagt cagtagcaat taatgggatt atcacaagta
     1561 tattaatgcg tgctaagccc catgaagtaa aactgatgat gatcgatccg aaaatggttg
     1621 aattgaatat gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa
     1681 aagcagcgca agccttacaa aaagtagtac aagaaatgga agaacgatat gaaaaattcg
     1741 cggcaaccgg agtaagaaat atttctggtt acaatgaatt tgttcaacag aaaaacttgg
     1801 aaaacggaac gaaacatcca accttaccat tcatcgtagt gatcgtagat gaattagctg
     1861 atttgatgat ggtagccagc aatgaagttg aagatgcgat tattcgttta gcgcaaatgg
     1921 ctcgggcagc ggggattcat atgatcctag ctactcaacg tccaagtgtg gatgttatca
     1981 caggaatcat caaagcaaat gtaccttcca gaatggcatt tgcggtatca agcggtacag
     2041 attctcggac gatcattgat tctaatgggg cagaaaagct cttagggcga ggagatatgc
     2101 tattcttgcc aatgggtgag aataaaccga tccgtgtgca aggagcattc atttctgacc
     2161 acgaagtaga acgggtcgtt caatttgtta cagatcagca agaagctcat tatgaagaga
     2221 aaatgatgcc gacagatgaa gttgaaacga caggtgctcc agaacagcca caagatgagc
     2281 tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc
     2341 aaagaagatt tcggatcggt tacaaccgag cagcacgttt agtagatgaa ctggaagctc
     2401 atggagttgt tggcccgtca gaaggaagca agcctcggaa agtattcatt gagcaggaag
     2461 aaacatcaga aacgttgccg gacgataatg acatgtaaaa cttgtgtgat gaagggttga
     2521 aaaacgaata accaataaaa agacaaggca tgatgatacc aaaagaaaaa atggtttttt
     2581 tccatctttt ctaatacggt atgatgtctt gtctttttag tttttgtgag attaaagttc
     2641 tattcgattt atttgtgaag tattgcatta aggaaatgaa tcatgtatag tatgtagcga
     2701 aactttgaga aaaagaggga aaatactttg gaaaaacagt ccatttatgg cttgacgaat
     2761 gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt
     2821 tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa
     2881 caattgatcg aaaaattatc ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc
     2941 caagaagctt cggatggtac agtaaaatat ttgtttgaac tccctgataa aaacatgatt
     3001 gagactgtac tgatgagaca agaatatggc ttgtcagttt gtgtgacgac acaagtagga
     3061 tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca aaaagaaccg tgatctgact
     3121 gctggagaaa ttgttgcaca gatcatgatg gtccaacatt attttgatga gcgcaatctt
     3181 ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg agccatttga taattacgac
     3241 aatgtaatgg actttttgca tatcatcaat gatgccaaag gattggcaat tggtgctcgg
     3301 cacattacag tttccactag tggattagca cacaaaatca aggaattcgc taataacggc
     3361 ttgcaagtca atctggctat ttccttgcac gcgccgaata atgaggtgcg aacatcgatc
     3421 atgcggatca accgaagttt cccaattgag aaattgatgg aagctgtgga tgagtattta
     3481 gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt tgaatcaagt aaatgataga
     3541 ccagagcatg cgcaacaatt agcagatcta ttaaaagata agaaaaaatt agcatatgtc
     3601 aacttgattc catataatcc agtcagtgaa catgatcaat attctcgcag tccaaaagct
     3661 gatgtattga gattttatga tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa
     3721 gaacatggta cagatattga tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa
     3781 acagttgcca aataatcgta tcctactcgt tttttcaatt accataaaag gagcagtttt
     3841 cttaattaag gaactgttca acttaaataa gagaaaagat tctatagcga aagctataaa
     3901 gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc
     3961 ctgcttgatg ggggttttag caattattta actgattttc ttatatcctt gctggctttc
     4021 ttgtgtagca tgataagttt ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat
     4081 ttcataaggc acatgtttaa tatcatttgt tgccatggct ttatctaaga ttgcttctaa
     4141 atcggtcgtg acattgagat tcatgtcggt taacgatact ggaagttggt tctttttcat
     4201 aaacacatag attttatcaa acaaatcgaa ctgctgatcc atcatcaata aggtcagtac
     4261 accataactg acagatgctc cgtgaaggta tccctccatt ttttctagaa cagtcgtacc
     4321 gtaatagaat gagtgtgcca tagcagaatt atagttattg ttaactaaaa cagaaaccaa
     4381 tccggtagta acaatgatat ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc
     4441 ggcactatct ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc acatttttcc
     4501 gatttggatt cctaatctat ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga
     4561 ttcgacttct ttggataaag catcgccaat acctgcccat aagtatttat caggtgcttc
     4621 ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc
     4681 gtccattacg ccttcttcgt tatagatgac acataccgca gtgacgggcg aacagttcga
     4741 accaatagtg ggaaaggtga agactggttt agctagtttt tcaccaatga cttttacagt
     4801 atcgcaagca cggccaccac cgactgcaaa caggacgtct gctttttgga cactttcatg
     4861 ctggatcaac tgttcaatat ttccataagt tgaattaccc ccgtaccaga gactggcgac
     4921 aacctctatt tcctgtgtca aagctacttt tattttgttt tctgcctttg aaagtgcagt
     4981 cttccctcca atcagcacta cttttgtccc atattctttg acgattccat ttgctttttc
     5041 atacgcatct gttccaatgg tataatttgg taagaaaaca ctttgactca tattcatttc
     5101 cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt
     5161 agatggagta gaggtgaacc taagacagga taagtgatga ataaggtggc atgaacacct
     5221 tgaaatcatc aataagtatc ctaaaaacga acaagtacaa aaacattgaa aacgacaata
     5281 atgaattttt gttcctctta gtatttctac cgattccctt tgcttttttg taattatcta
     5341 aaaattctaa aaattaagat tattatatga aaacaggttt tatttgtcaa ttcataattg
     5401 gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca
     5461 attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat
     5521 tttataagta agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg
     5581 agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga
     5641 cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat
     5701 ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa
     5761 atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt
     5821 attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa
     5881 agctcccgac gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta
     5941 gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg
     6001 ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt
     6061 cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca
     6121 aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca
     6181 aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga
     6241 atttacgaaa attactt
//
LOCUS       DACEDO010000209         3738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.guided.137, whole genome shotgun sequence.
ACCESSION   DACEDO010000209 DACEDO010000000
VERSION     DACEDO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.guided.137"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            408..1886
                     /gene="msr"
                     /locus_tag="IWO52_002953"
     CDS             408..1886
                     /gene="msr"
                     /locus_tag="IWO52_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0357145.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1933..2379
                     /locus_tag="IWO52_002954"
     CDS             1933..2379
                     /locus_tag="IWO52_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0357146.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2578..3093)
                     /locus_tag="IWO52_002955"
     CDS             complement(2578..3093)
                     /locus_tag="IWO52_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0357147.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3210..>3738
                     /locus_tag="IWO52_002956"
     CDS             3210..>3738
                     /locus_tag="IWO52_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0357148.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYP"
BASE COUNT         1165 a          627 c          803 g         1143 t
ORIGIN      
        1 gaaggttacg agtctgttcg tttagcacca gaaaagatca ctatgttcga ataaaaggtt
       61 ttcagttact attcagtagc tattgctttt tttgaatcgt gtgtggtaac ctaataaaaa
      121 aggaggtcgg aaaaatgact gcatcgatga aattacgttt cgaacttttg aataacaact
      181 aaataaattc aaaagacatg tataaacaac aatttcatgg atggagtatg tccatttttt
      241 cgaacgacag tagaataggt gaaagctatg acagatttgg tgatttagtc atagttattt
      301 cgttcagagt aggaaaagat gtgatgcagt ttttttgcaa acagattttt ctgctctttt
      361 ttagtatcga taattgttgt tttacatgaa ataggagtgt aaaacatatg gaaaatttag
      421 cagtaaatat aacaaatcta caagtcagct ttggcaacca gctagaacta tctattgatt
      481 ctcttcgtgt ctatcagcaa gatcgtatag ggatcatagg agaaaatggt gtcggtaaat
      541 cgactttgct caaactaata gccggtgaac tttttcccga tcatgggaaa atccaaacag
      601 agatcacctt caactacctg cctcaattaa cctatctcgc tgaagcaaag gacctaaatt
      661 tggaattagc cagtcatttc cagttaagac tggaagaaac ttcagagcgg aaatggagcg
      721 gaggggaaga acgaaagatc gagttgatac gtcttctttc ttcttatgaa caagggatgc
      781 ttctagacga gccaacaacc catctagata gaaaaagtat tgatcgactg attgaagagc
      841 ttcgttatta ttatggtacg ctggtttttg ttagtcatga tcgctatttt ctagatgagc
      901 tagcatcgaa aatctgggaa gtaaaagacg gagaaatccg agagttttcg gggaattata
      961 gtgcctatct cactcaaaag gaattggaga aaaagactca gctacgagaa gcagagtcga
     1021 tcatgaaaga gaaaaaacga ttggaaaaat cgatccaaga aaagaaaaaa caagcggaaa
     1081 agttggaaaa agtgtccagt aaaaagaaaa agcaacaaat cagactggat cggttgtctt
     1141 cctctaaaca aaaagacagt gtacaaaaag ccatccaaaa gaatgcgaaa acattagaga
     1201 gaagactcca aaaaatagga gaaacaacca agccgcagca gatgaaacaa atccgttttc
     1261 cagtaccaaa atctcttgaa ctccacagcc gctatcccat catgggacaa aatgtccaat
     1321 tggaaagaag tgggagaaca ttactggtaa atggtgattt tcagttttct ttaggtaaaa
     1381 aaatcgcgat tgtcggtgaa aatggttcag gtaagacaac cttattagaa catatccgca
     1441 aacaaggaga aggaatcctt ctctctccga aagtaagctt tcaagtatat cagcaaaagg
     1501 gttatcaaat gacatctgaa gaatccatca ttcgttttgt catgagacaa acagagtttt
     1561 cagaatcgct tgtccgtagt ttgctgaatc acttagggtt tgctcaggaa actttgacga
     1621 aaccgttatg tacattaagt gggggagaag cgacccgtct gacgattgct ttgcttttta
     1681 ctaagccaag taatgtgttg ctgttagatg aaccgactaa ttttattgat atggcaacga
     1741 tcgaagcttt agagaagcta atgcaaatat atccgggaac gattttgttt acttctcatg
     1801 attcttactt tgtcgagcgt acggctgatg aagtttatga aataaaaggg cagaaaataa
     1861 aaaaagtact tacgagaaat ttttaaatgt gctacgatga ttttcgtgac aaatttaaag
     1921 gagtggtaca gattgcctac aaataaaaaa gaaggaataa tttttacaac aatgatgtgt
     1981 tttttgatgg tcttggggat gagcgcgtat aatttatggc tgcatcaaga tcttcattta
     2041 tcaagtttgt tgattggtct agtgcctgga ttcgtggtgg ctttcatttt ggatgtcttc
     2101 atcatcggtg tgctggcgaa aaaaattgcc tttaaactac cagtaaatcc agaaagcaag
     2161 gtccaaatga tcttaactat ttcatgcttg atggttttag gaatggtgac atgtatgtct
     2221 ttattcggtg tgatcatgga acaaggaata cctgatcaat tattctctgc ttatatgaat
     2281 gcttggaaaa tgaatgtgat tgccgcttta ccgttgcagt tgatcatcgt tggcccgctt
     2341 tcaagaagtg tgctaaaagc aattcaaagt aatgaatgat ggaacactgt tatttgaaaa
     2401 aaggccttgg catgcttttt gtcatagtct cttgaatgca gttctagaaa aatagtgatc
     2461 cgaacgatag gttttggcct gtcgttcgga tcgctttttc ttttccaact gtattgctct
     2521 tttattgaag ataatttgct cttcaataga tatttctaga aaggttctgt ttatccctta
     2581 ctcaaaaaac tgctgcattt gttctcttac ttgctgacac cattggagtt cctgctgaca
     2641 aagcataatt ctgcgctgga tcacaacata acgtccaaat tcttgtttct tgtctagcga
     2701 gaggtcattc aatttttttt gatttttttg catgattttg ttcagctgtt gttctctagt
     2761 gaatagcagt ccagtagctg ttgttttatc gagcgtcgaa aaagcataga tttttgctag
     2821 aaattcatct ttttgcgtag gagcgggagt ttcctctttg atccattcct ctacaagagc
     2881 acgtccttca tcagtcaagt ggtagatctt tttttgagag tgctcgtctt tgtccaaaac
     2941 ggtcacataa ccctgctggt ctaacttggc aagcgttgta tagatctgac tgtgatgggc
     3001 ttcccaaaaa attgtgatca gctgcttcaa ttcataccca gaacgtggtt tggataaaag
     3061 cacgcataat aatatatagg ctaaagtatt cataaaaatc ctacctctcg tcttattttc
     3121 tttattatat cacagtaaag tttccacgtt ttacatgtgc atattgacat gtaaaataaa
     3181 tcatgtatga tgctaataga gagaaaggga tggaaatatt ggagaatcaa atagatatac
     3241 aagaacaaac aaatgaagaa acaaagggga aaagttcagc gctagttgta gcagctacct
     3301 gtatgatcgc ttttatggga gtgggtctag tagacccaat cttaaaaacg attgctgcac
     3361 agttaaacgc tacgccagct gaaacgaccc tgttatttac tagttacatg ttagtcactg
     3421 gtgtggtgat gctatttaca ggtttcttat ctagtcggat cggtttgaaa aaaacattga
     3481 tgacaggatt gtttatcatc gttgtattcg ctggtcttgg cggattatct gggaatatcg
     3541 gaatgctgat tgggttacgt gccggctggg gtgtcgggaa tgctttattt gtctctactg
     3601 ctttagcaac gatcgtcagt attttagttg gtaacacaga aaaagcaatc atgatgtatg
     3661 aagcagcact tggctgcgga atggctgttg gccctctagt cggtggggtc ttgggcagtg
     3721 gttcatggcg ttatccat
//
LOCUS       DACEDO010000210         3235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.guided.141, whole genome shotgun sequence.
ACCESSION   DACEDO010000210 DACEDO010000000
VERSION     DACEDO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.guided.141"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..202
                     /locus_tag="IWO52_002957"
     CDS             <1..202
                     /locus_tag="IWO52_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0357149.1"
                     /translation="IQHLKFQKLLDRYDDLYSMDMFGESIEGHKYKTPYQVVKMINPN
                     SKEDMELLREVLRNNNFDDEDE"
     gene            356..433
                     /locus_tag="IWO52_002958"
                     /pseudo
     CDS             356..433
                     /locus_tag="IWO52_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            678..794
                     /locus_tag="IWO52_002959"
     CDS             678..794
                     /locus_tag="IWO52_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0357150.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            810..2729
                     /gene="tet(M)"
                     /locus_tag="IWO52_002960"
     CDS             810..2729
                     /gene="tet(M)"
                     /locus_tag="IWO52_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0357151.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2848..3015
                     /locus_tag="IWO52_002961"
     CDS             2848..3015
                     /locus_tag="IWO52_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0357152.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(3075..>3235)
                     /locus_tag="IWO52_002962"
     CDS             complement(3075..>3235)
                     /locus_tag="IWO52_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0357153.1"
                     /translation="VSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLMESLASGI
                     NEARNIED"
BASE COUNT         1111 a          481 c          650 g          993 t
ORIGIN      
        1 tatccagcat ttgaagtttc aaaaattatt agaccgctat gatgatttat acagtatgga
       61 tatgtttggg gaaagcatag agggacataa gtataagaca ccttatcaag tcgtgaaaat
      121 gataaacccg aactcaaaag aagatatgga actgttacga gaagtattaa ggaataacaa
      181 ttttgatgat gaggacgagt aacttttttc ctcttgcgag aagatacagc aaacaaaaag
      241 cccgatcaag agtgcaaagc accaccaatg gtggcaaagc tcttgacagg gctttttctt
      301 ttctgttgtg ggcaatcaag aggaagaaag ggataaaatc tgatttgtgg taaatcaggc
      361 aaaggcaacg caggtatctc aatttgattt ggtacttgaa aagaacggga gtaattggaa
      421 gattatagaa taacaaatat tggtacatta ttacagctat tttgtaatca cgtactctct
      481 ttgataaaaa attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtatc
      541 tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg tcaatttgat
      601 agtgggaaca aataattgga tgtccttttt taggagggct tagttttttg tacccagttt
      661 aagaatacct ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc
      721 ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat ttttatgccc
      781 ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc
      841 atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta
      901 cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc
      961 agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga
     1021 acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag
     1081 ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta
     1141 tattatttca tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg
     1201 accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg
     1261 aaattgtaat caaacagaag gtagaactgt atcctaatat gtgtgtgacg aactttaccg
     1321 aatctgaaca atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt
     1381 ccggtaaatc attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt
     1441 gttctctgtt ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta
     1501 tagaagttat tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg
     1561 gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt
     1621 atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag
     1681 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
     1741 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
     1801 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
     1861 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
     1921 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
     1981 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
     2041 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
     2101 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
     2161 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
     2221 aatcgtttca aaatgcagtt atggagggga tacgctatgg ctgtgaacaa ggattgtatg
     2281 gttggaatgt gacggactgt aaaatctgtt ttaagtatgg cttatactat agccctgtta
     2341 gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag
     2401 ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca caggaatatc
     2461 tttcacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac actcaattga
     2521 aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa gaatatcgta
     2581 gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta aaagggtacc
     2641 atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg atagataaag
     2701 tacgatatat gttcaataaa ataacttagt gtattttatg ttgttatata aatatggttt
     2761 cttgttaaat aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggag
     2821 atagttatta taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta
     2881 aagataaggg aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa
     2941 gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag
     3001 acgcagagcc gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt
     3061 atggattctt ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat
     3121 tccattaagg atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt
     3181 cgggtgcttt ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacat
//
LOCUS       DACEDO010000211         2634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.guided.146, whole genome shotgun sequence.
ACCESSION   DACEDO010000211 DACEDO010000000
VERSION     DACEDO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.guided.146"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..713
                     /locus_tag="IWO52_002963"
     CDS             <1..713
                     /locus_tag="IWO52_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0357154.1"
                     /translation="AINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLI
                     LRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKK
                     QAEIYPTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFAC
                     NNAYCINEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIV
                     SEINNFLNEESSDERKQI"
     gene            694..1428
                     /locus_tag="IWO52_002964"
     CDS             694..1428
                     /locus_tag="IWO52_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0357155.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1461..2369
                     /locus_tag="IWO52_002965"
     CDS             1461..2369
                     /locus_tag="IWO52_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ0357156.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2366..2614
                     /locus_tag="IWO52_002966"
     CDS             2366..2614
                     /locus_tag="IWO52_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAQ0357157.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT          920 a          368 c          588 g          758 t
ORIGIN      
        1 cagcgattaa tcaaattgct acagagttgg atgatgagaa tagaaacaac cttgttgtac
       61 ctcccggagc gtggggtgat tgggttaatg gcggtggatg gttagttata aacgggtgtc
      121 atgttgactt gatattacgt gatataaaac gggtggaaca aataatcaaa gatacggagc
      181 aaggaattgt tactgccaat tatcagaccg ggcatcccca tggttatata agtgctatgt
      241 atcgtggaga attagcgatt agtaaaatac tatatgctaa gaacgaaagc ttatgcgaat
      301 taaaaaagca ggcagaaatt taccctactg ctctaaagaa aagcttgatg aactttttta
      361 tatttgaagc tgaattctct ttaatgtttg taaaagcaaa tgcgggggca gaggataaat
      421 attatattgc aggccatgtt tttcgtatta tttcatgctt aaatcaagta ctatttgcat
      481 gtaataatgc ttattgcatt aacgaaaaga aagctataaa actgcttgaa acttttgaat
      541 ataaacctga aaaatatgcc gaaagagtta atcatatttt tgaagtactc ggtctttctc
      601 tttttgaatg ctacgacatg accgagaaac tttataaaga agtgaatgaa attgtatcgg
      661 agataaataa ctttttaaac gaggagagtt cagatgaaag aaaacaaata tgatgataat
      721 atattttttc aaaaatacag tcaaatgagt cgctcgcaga aaggactggc tggtgcggga
      781 gaatgggaga ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt gcttgattta
      841 ggatgcggct atggatggca ctgtatatat gcgatggaaa acggtgcttc ctctgtagta
      901 ggtgttgata tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca ttttccacag
      961 attgaatatg aatgctgtgc catagaagat gtggatttcc cagaggagag ctttgatgta
     1021 atactaagtt cgcttgcgtt tcattatgta gcagactatg agaatttaat aaaaaagata
     1081 tataggatgc tgaaggctgg tggcaattta gtttttacag ttgaacatcc tgtttttact
     1141 gctcatggaa cacaagactg gtattataac gaaaaaggag aaatactgca tttcccggtg
     1201 gacaattatt attatgaggg caaacggaca gctatgtttt tggaagaaaa ggttacaaaa
     1261 tatcatagaa cactgaccac atatctaaat acactgcttt caaatagttt tataataaat
     1321 cagattgtgg agccacagcc gccagagaac atgatggata ttccggggat ggcggatgaa
     1381 atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga agatgtaata atatagaaaa
     1441 aataaacgag gagtatgtaa atgagatcag aaaaagaaat gatggattta gtactttctt
     1501 tagcagaaca ggatgaacgt attcgaattg tgacccttga ggggtcacgc gcaaatatta
     1561 atatacctaa agatgaattt caggattatg atattacata ttttgtaagt gatatagaac
     1621 cgtttatatc taatgatgac tggcttaatc aatttgggaa tataataatg atgcaaaagc
     1681 cggaggatat ggaattattc ccacctgaag aaaagggatt ttcctatctt atgctatttg
     1741 atgattacaa taaaattgat cttaccttat tgcccttgga agagttagat aattacctaa
     1801 agggcgataa attaataaag gttctaattg ataaagattg tagaattaaa agggacatag
     1861 ttccgactga tatagattat catgtaagaa agccaagcgc aagggagtat gatgattgct
     1921 gcaatgaatt ttggaatgta acaccttatg ttattaaagg attgtgccgt aaggaaattt
     1981 tatttgctat tgatcatttt aatcagattg ttcgccatga gctgctgaga atgatatcat
     2041 ggaaggtcgg catcgaaaca ggctttaaat taagtgtagg caagaactat aagtttattg
     2101 aaaggtatat atccgaggat ttgtgggaga aacttttgtc cacctaccgg atggattcct
     2161 atgaaaacat atgggaagca ttatttctat gccatcaatt gttcagggcg gtatccggtg
     2221 aggtggcgga aaggcttcat tatgcctatc cggagtatga taggaatata acaaaatata
     2281 ccagggacat gtataaaaaa tacactggta aaaccggctg cctggatagc acatatgccg
     2341 ctgatataga agagaggcgg gaacagtgat tacagaaatg aaagcagggc acctgaaaga
     2401 tatcgataaa cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa
     2461 cgagaattgg acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga
     2521 cgaagaggat gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa
     2581 gataatatat cttttaaccc gtggtgctag ttaattttaa tatcctaaag aata
//
LOCUS       DACEDO010000212         1552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.guided.166, whole genome shotgun sequence.
ACCESSION   DACEDO010000212 DACEDO010000000
VERSION     DACEDO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.guided.166"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            247..1122
                     /locus_tag="IWO52_002967"
                     /note="aph(2'')-Ia"
     CDS             247..1122
                     /locus_tag="IWO52_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ0357158.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1252..>1552)
                     /locus_tag="IWO52_002968"
     CDS             complement(1252..>1552)
                     /locus_tag="IWO52_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0357159.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          619 a          149 c          262 g          522 t
ORIGIN      
        1 aaaactttgc aacagaaccg tacaagatat attaaattga tttttgaatt tttgaaaaaa
       61 gaaagaaatg ctaatgcagt tattttagac cctcataaaa ataatccaag agcaataagg
      121 gcataccaaa aatctggttt tagaattatt gaagatttgc cagaacatga attacacgag
      181 ggcaaaaaag aagattgtta tttaatggaa tatagatatg atgataatgc cacaaatgtt
      241 aaggcaatga aatatttaat tgagcattac tttgataatt tcaaagtaga tagtattgaa
      301 ataatcggta gtggttatga tagtgtggca tatttagtta ataatgaata catttttaaa
      361 acaaaattta gtactaataa gaaaaaaggt tatgcaaaag aaaaagcaat atataatttt
      421 ttaaatacaa atttagaaac taatgtaaaa attcctaata ttgaatattc gtatattagt
      481 gatgaattat ctatactagg ttataaagaa attaaaggaa cttttttaac accagaaatt
      541 tattctacta tgtcagaaga agaacaaaat ttgttaaaac gagatattgc cagtttttta
      601 agacaaatgc acggtttaga ttatacagat attagtgaat gtactattga taataaacaa
      661 aatgtattag aagagtatat attgttgcgt gaaactattt ataatgattt aactgatata
      721 gaaaaagatt atatagaaag ttttatggaa agactaaatg caacaacagt ttttgagggt
      781 aaaaagtgtt tatgccataa tgattttagt tgtaatcatc tattgttaga tggcaataat
      841 agattaactg gaataattga ttttggagat tctggaatta tagatgaata ttgtgatttt
      901 atatacttac ttgaagatag tgaagaagaa ataggaacaa attttggaga agatatatta
      961 agaatgtatg gaaatataga tattgagaaa gcaaaagaat atcaagatat agttgaagaa
     1021 tattatccta ttgaaactat tgtttatgga attaaaaata ttaaacagga atttatcgaa
     1081 aatggtagaa aagaaattta taaaaggact tataaagatt gattatataa tatatgaaaa
     1141 gctattataa aagacattag tattaaatag tttaaaaaaa tgaaaaataa taaaggaagt
     1201 gagtcaattc cagactcctg tgtaaaatgc tatacaatgt ttttaccatt tctacttatc
     1261 aaaattgatg tattttcttg aagaataaat ccattcatca tgtaggtcca taagaacggc
     1321 tccaattaag cgattggctg atgtttgatt ggggaagatg cgaataatct tttctcttct
     1381 gcgtacttct tgattcagtc gttcaattag attggtactc tttagtcgat tgtgggaatt
     1441 tccttgtacg gtatattgaa aggcgtcttc gaatccatca tccaatgatg cgcaagcttt
     1501 tgaatatttt ggttggttct gttgcaaagt tttaaatcta ctatcaaata ag
//
LOCUS       DACEDO010000213         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.guided.167, whole genome shotgun sequence.
ACCESSION   DACEDO010000213 DACEDO010000000
VERSION     DACEDO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.guided.167"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWO52_002969"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWO52_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWO52_002970"
     CDS             394..477
                     /locus_tag="IWO52_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0357160.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO52_002971"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO52_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0357161.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACEDO010000214         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E51507_2017
            SAMN12639820-rid9879663.guided.172, whole genome shotgun sequence.
ACCESSION   DACEDO010000214 DACEDO010000000
VERSION     DACEDO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639820
            Sequence Read Archive: SRR10023204
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741729.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741729.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:33:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,860
            CDSs (with protein)               :: 2,860
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 12 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639820-rid9879663.guided.172"
                     /isolate="E51507_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            45..296
                     /locus_tag="IWO52_002972"
                     /pseudo
     CDS             45..296
                     /locus_tag="IWO52_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            389..1183
                     /locus_tag="IWO52_002973"
                     /note="aph(3')-IIIa"
     CDS             389..1183
                     /locus_tag="IWO52_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0357162.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          233 c          322 g          369 t
ORIGIN      
        1 attttcctct aggaaaaaat taactagcac cacgggttat cttttatata gaagatatcg
       61 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
      121 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
      181 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
      241 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
      301 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
      361 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
      421 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      481 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      541 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      601 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      661 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      721 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      781 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      841 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
      901 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
      961 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     1021 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1081 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1141 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     1201 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
     1261 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagatagg ggtcccgagc
     1321 gcctacgagg aatttgtatc gataagaa
//
